LOCUS       DAVHIU010000001        50469 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVHIU010000001 DAVHIU010000000
VERSION     DAVHIU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.001"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     regulatory      32..120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            336..1004
                     /locus_tag="VVN06_000001"
     CDS             336..1004
                     /locus_tag="VVN06_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU5561625.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1241..2023
                     /gene="thiM"
                     /locus_tag="VVN06_000002"
     CDS             1241..2023
                     /gene="thiM"
                     /locus_tag="VVN06_000002"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU5561626.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            2025..2654
                     /locus_tag="VVN06_000003"
     CDS             2025..2654
                     /locus_tag="VVN06_000003"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5561627.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2863..2951
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3213..3773
                     /locus_tag="VVN06_000004"
     CDS             3213..3773
                     /locus_tag="VVN06_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5561628.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3774..5159
                     /locus_tag="VVN06_000005"
     CDS             3774..5159
                     /locus_tag="VVN06_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561629.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLSHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5161..5811
                     /locus_tag="VVN06_000006"
     CDS             5161..5811
                     /locus_tag="VVN06_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU5561630.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSLANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5822..6514
                     /gene="tenA"
                     /locus_tag="VVN06_000007"
     CDS             5822..6514
                     /gene="tenA"
                     /locus_tag="VVN06_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU5561631.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFYISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6519..7043
                     /gene="thiW"
                     /locus_tag="VVN06_000008"
     CDS             6519..7043
                     /gene="thiW"
                     /locus_tag="VVN06_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU5561632.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            7043..7846
                     /locus_tag="VVN06_000009"
     CDS             7043..7846
                     /locus_tag="VVN06_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU5561633.1"
                     /translation="MQEFTNPFPIDSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            7839..8471
                     /locus_tag="VVN06_000010"
     CDS             7839..8471
                     /locus_tag="VVN06_000010"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5561634.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8680..9471)
                     /gene="thiD"
                     /locus_tag="VVN06_000011"
     CDS             complement(8680..9471)
                     /gene="thiD"
                     /locus_tag="VVN06_000011"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5561635.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9555..9653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9837..10232
                     /locus_tag="VVN06_000012"
     CDS             9837..10232
                     /locus_tag="VVN06_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU5561636.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKFFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10243..10614
                     /locus_tag="VVN06_000013"
     CDS             10243..10614
                     /locus_tag="VVN06_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5561637.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEIMPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            10624..12867
                     /locus_tag="VVN06_000014"
     CDS             10624..12867
                     /locus_tag="VVN06_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5561638.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13074..14849
                     /gene="spxB"
                     /locus_tag="VVN06_000015"
     CDS             13074..14849
                     /gene="spxB"
                     /locus_tag="VVN06_000015"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU5561639.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            14960..15307
                     /locus_tag="VVN06_000016"
     CDS             14960..15307
                     /locus_tag="VVN06_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5561640.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(15424..16470)
                     /locus_tag="VVN06_000017"
                     /pseudo
     CDS             complement(15424..16470)
                     /locus_tag="VVN06_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16535..16831
                     /locus_tag="VVN06_000018"
     CDS             16535..16831
                     /locus_tag="VVN06_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU5561641.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGVLKRSKKKSFYWYKDVIDSNGASIE"
     gene            16952..17896
                     /gene="manA"
                     /locus_tag="VVN06_000019"
     CDS             16952..17896
                     /gene="manA"
                     /locus_tag="VVN06_000019"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU5561642.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(17917..19248)
                     /locus_tag="VVN06_000020"
     CDS             complement(17917..19248)
                     /locus_tag="VVN06_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU5561643.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMMP
                     LLFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILG
                     ILTFVFGIPSALSYGVMADVHIFGKIFFDAMDFLASNLLMPFGALYLSLFTGYIFKKA
                     LAMEELHLDERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            19331..20085
                     /locus_tag="VVN06_000021"
                     /pseudo
     CDS             19331..20085
                     /locus_tag="VVN06_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(20105..20560)
                     /locus_tag="VVN06_000022"
                     /pseudo
     CDS             complement(20105..20560)
                     /locus_tag="VVN06_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(20580..21755)
                     /locus_tag="VVN06_000023"
     CDS             complement(20580..21755)
                     /locus_tag="VVN06_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5561644.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(21814..22659)
                     /locus_tag="VVN06_000024"
     CDS             complement(21814..22659)
                     /locus_tag="VVN06_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5561645.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            22784..23341
                     /locus_tag="VVN06_000025"
     CDS             22784..23341
                     /locus_tag="VVN06_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5561646.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            23360..23827
                     /locus_tag="VVN06_000026"
     CDS             23360..23827
                     /locus_tag="VVN06_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU5561647.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            23916..24545
                     /gene="upp"
                     /locus_tag="VVN06_000027"
     CDS             23916..24545
                     /gene="upp"
                     /locus_tag="VVN06_000027"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5561648.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            24719..25309
                     /gene="clpP"
                     /locus_tag="VVN06_000028"
     CDS             24719..25309
                     /gene="clpP"
                     /locus_tag="VVN06_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU5561649.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            25388..25636
                     /locus_tag="VVN06_000029"
     CDS             25388..25636
                     /locus_tag="VVN06_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU5561650.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            25738..26898
                     /locus_tag="VVN06_000030"
     CDS             25738..26898
                     /locus_tag="VVN06_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5561651.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            27166..28035
                     /locus_tag="VVN06_000031"
     CDS             27166..28035
                     /locus_tag="VVN06_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5561652.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            28039..28995
                     /locus_tag="VVN06_000032"
     CDS             28039..28995
                     /locus_tag="VVN06_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5561653.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            28995..29759
                     /locus_tag="VVN06_000033"
     CDS             28995..29759
                     /locus_tag="VVN06_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561654.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            29759..30469
                     /locus_tag="VVN06_000034"
     CDS             29759..30469
                     /locus_tag="VVN06_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561655.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISNRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            30883..31539
                     /locus_tag="VVN06_000035"
     CDS             30883..31539
                     /locus_tag="VVN06_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5561656.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNYQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            31647..32742
                     /gene="prfB"
                     /locus_tag="VVN06_000036"
     CDS             join(31647..31718,31720..32742)
                     /gene="prfB"
                     /locus_tag="VVN06_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5561657.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            32760..33452
                     /gene="ftsE"
                     /locus_tag="VVN06_000037"
     CDS             32760..33452
                     /gene="ftsE"
                     /locus_tag="VVN06_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5561658.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            33445..34371
                     /locus_tag="VVN06_000038"
     CDS             33445..34371
                     /locus_tag="VVN06_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5561659.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLRI"
     gene            34657..36837
                     /locus_tag="VVN06_000039"
     CDS             34657..36837
                     /locus_tag="VVN06_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5561660.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTLFGGSIKVADYFISVMEAPALNMGVFVGIISGFIGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSIIAAIVLAAFWPVVQTGINSFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVLTGAAKGSQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVVVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFVATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEESSNEVKVAAGSQAVNIINLLGGRANIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADVLKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKGLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHALGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVVAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            36889..37704
                     /locus_tag="VVN06_000040"
     CDS             36889..37704
                     /locus_tag="VVN06_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5561661.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEI
                     TSPEVEVNDLYQALPAAELIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            37844..39187
                     /locus_tag="VVN06_000041"
     CDS             37844..39187
                     /locus_tag="VVN06_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5561662.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKIVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            39564..40754
                     /locus_tag="VVN06_000042"
     CDS             39564..40754
                     /locus_tag="VVN06_000042"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5561663.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            41307..42242
                     /locus_tag="VVN06_000043"
     CDS             41307..42242
                     /locus_tag="VVN06_000043"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU5561664.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            42276..43313
                     /locus_tag="VVN06_000044"
     CDS             42276..43313
                     /locus_tag="VVN06_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5561665.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            43486..44091
                     /locus_tag="VVN06_000045"
     CDS             43486..44091
                     /locus_tag="VVN06_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU5561666.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            44248..44778
                     /locus_tag="VVN06_000046"
     CDS             44248..44778
                     /locus_tag="VVN06_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU5561667.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            44799..45701
                     /locus_tag="VVN06_000047"
     CDS             44799..45701
                     /locus_tag="VVN06_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEU5561668.1"
                     /translation="MKPSIHSLAHQTMQKWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            45701..46183
                     /locus_tag="VVN06_000048"
     CDS             45701..46183
                     /locus_tag="VVN06_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5561669.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VFLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            46185..47726
                     /locus_tag="VVN06_000049"
     CDS             46185..47726
                     /locus_tag="VVN06_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5561670.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(47782..48585)
                     /locus_tag="VVN06_000050"
     CDS             complement(47782..48585)
                     /locus_tag="VVN06_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5561671.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDTLTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(48669..48881)
                     /locus_tag="VVN06_000051"
     CDS             complement(48669..48881)
                     /locus_tag="VVN06_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561672.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(49360..49569)
                     /locus_tag="VVN06_000052"
     CDS             complement(49360..49569)
                     /locus_tag="VVN06_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561673.1"
                     /translation="MLSVNTILEKFYKKHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            49739..50011
                     /gene="rpsP"
                     /locus_tag="VVN06_000053"
     CDS             49739..50011
                     /gene="rpsP"
                     /locus_tag="VVN06_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5561674.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            50031..50270
                     /locus_tag="VVN06_000054"
     CDS             50031..50270
                     /locus_tag="VVN06_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU5561675.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT        15204 a         9359 c        10979 g        14927 t
ORIGIN      
        1 tttttgctat actggttcag taattaaata atagagacat ttggggtgct ttaagctgag
       61 atgataccca ttgaacctga tacagttaag actggcgaag ggaaatgtga aacgtttttt
      121 cgtatggcga aatgaacgat ctaatcttgt aagtcaactc taattcttga ttgtaattgg
      181 agttttttgt ttatataaac tggataggct tgtttagtta gcttctctgc aaatgctctc
      241 gatgtgcttt aaaaggatca agtggaatta cgattttgct tgatatttct tggtgtttta
      301 ttaattacta acaagtaaag agaggagaag taaatatgga aacacaagac tatgcatttc
      361 agccaggttt gactgttgga gaattattaa aaagcagtca gaaggattgg caggctgcaa
      421 tcaatcatcg ttttgttaag gaactttttg cggggacaat tgagaataag gtcttaaagg
      481 actatctgat tcaagattat cacttctttg atgccttctt atccatgctg ggtgcctgcg
      541 tagctcacgc agataaactt gaatctaagc ttcgttttgc caagcaacta ggctttcttg
      601 aagcagatga agatggttat ttccaaaagg cttttaaaga gttaaaagta gcagagaatg
      661 actatctaga agtgactttg caccctgtaa caaaagcctt tcaggatcta atgtattcgg
      721 cggtggcttc atcagactat gcccatcttt tggtcatgct ggtcattgca gaaggtctct
      781 atttagactg gggttctaaa gatttggctt tacctgaagt ctatattcat tcggaatgga
      841 tcaatctcca cagaggtcct ttctttgcag agtgggttca atttctggtt gacgaactca
      901 atcgtgtcgg taaaaatcga gaagatttga cagaacttca gcaacgctgg aatcaagcag
      961 tcgctttaga attagctttt tttgatattg gttacgatgt ctagagaagg ctcatgatat
     1021 ttacaaattt gatgaggtaa gactcttcga aaatctcttc gaaccacgtc agttctatcc
     1081 acaacctcaa aacagtgttt tgagcaacct gcggttagcc tcctagtttg ctctttgatt
     1141 ttcattgagt ataaatcatt caatgtaaat gaaaaaaatt ctcaactaaa gataaatcga
     1201 aatcagtaga tcgtataaag tgaaaaggaa ggatttcaat atgacaagtt taaaattatt
     1261 aaaagaaaaa gcaccattgg tcatttgtat aaccaatgat gtagtaaaaa atttcacagc
     1321 aaatggatta gtagcactgg gcgcctcacc agccatgagt gagtttccag cagatttaga
     1381 ggatttgtta aagtatgctg gtggtttatt aataaacata ggaacattga cagatgaaaa
     1441 ttggaaatta taccaagctg ctctgaaaat tgcggagaaa tataatgtcc cagcagtttt
     1501 agatcctgta gcctgtggag caggagaata tagaaaaaaa gtagcagatg atctaatcaa
     1561 caattataaa ctagcagcga ttagaggaaa tgctggcgag attgcctctt tagtaggaat
     1621 agatgtggca tctaaaggag tagatagtgc gggcgtagat aatattgacg aaattgctct
     1681 agcagcaaat gagaagttca atattccaat agtagtaaca ggtgaagtgg atgccattgc
     1741 tgttaatgga gaagtggtaa cgattcataa tggtagtgct atgatgccga aagtcattgg
     1801 gacaggatgc ttattaggag ctgtagtagc aagctttatc ggactggaaa aaggtcaaga
     1861 attgaaatca ttagaaacag caatgttggt ttacaatatc gctggagaaa tagcagaaaa
     1921 acgtcccaat ggacatcttc ctggaacatt taaagctgaa tttataaatg ccttatacga
     1981 gattacagat gaagatgtaa aggaattcaa aagagtgaag taagatgttt cataaagaat
     2041 tactaaaact atattttatt tgtggaacga ctacttgcca aggaaaaaat ctatatacag
     2101 tcgttgagga agccttaaaa ggtggtataa ccttatttca atttcgagaa aaaggtgagg
     2161 gtgctttaga aggcttagaa aaacttgagc tagctatcca aattaaagaa ctttgtaaaa
     2221 aatacaatgt tccatttatc gttaatgacg atatagattt agcaatggaa attgacgccg
     2281 atggcgtaca tgtaggtcaa gatgatattg gtgttgatga aattagaaaa ttgatgccag
     2341 ataaaataat tggtctttct ataagaaacg aagaagaatt tcaacaatca aaagttgaat
     2401 atgtagatta tgtaggtgtt gggcctgtat ttgataccca gtcaaaagat gatgctggtg
     2461 gtgctatagg ttatgaaggt cttgaattga tgagaaaact attgccacaa atgcctttag
     2521 ttgcaattgg tgggatacag acgaaacata ttaaagacat tataaagacc aatgttgacg
     2581 gcgtttcaat catttcggca atatcgtatg caaaaaatat agaaaaaact gttcgggaaa
     2641 tgagtgaaca atagatatct atccttggat tctaagtaag taggattttc cttttttaaa
     2701 aagctatgtc tagattcagc ttgaatggct aattcatcaa acaggtcatt tgttttttgc
     2761 aaaagaaaga tctgctgtct ttgtgagtgc aaggctgtat ttccagttca atttcttgaa
     2821 ctagtataaa atgtgtgtta taataatcac ataattaaat aataaaggca tttggggtgc
     2881 tttaggctga gatgataccc attgaacctg atacagttaa gactggcgaa gggaaatgtg
     2941 aaatgttttt ttcgtatgtc gaaaagaact gtctaatctt gtaagctgaa ctctaattcg
     3001 tatctgtaat tagagttttt tcttttacga aactggatga agatgatata gtagattgaa
     3061 ataagatgcg aacaaatcaa ttagaaaatt caaattaatt tctagaaata ttttagcatt
     3121 cctgtgtact gttctaaatt caatctgcta tacaaagtat ttaggtcggt cttcttgagt
     3181 gccccacttt tcttttaaaa aaggagtttt ttatgttgaa aaaatggcag ttaaaagatg
     3241 ttatcttgct tgctttcttg tctatctttt ttggtggggt tttcgttggt tcaggatatg
     3301 tgtataatat tctcagtcta ctcttaacac ctcttggttt gcaggccttt gccaatgaaa
     3361 tcctcttcgg tctctggtgt atggctgcgc ccattgctgc catctttgtt ccgagagtcg
     3421 gaagtgcaac gattggagaa gtgctagctg cgcttgctga agtcctttat ggtagccaat
     3481 ttggtctagg agctcttttg tctggcttag ttcaaggttt gggaagtgaa tttggtttta
     3541 tcgtaactaa gaatcgctat gaaagttggc tctctctaac tgctaatagt attgggatta
     3601 cgcttgttag ctttgtctat gaatacatta agttaggtta ctacgccttt tcccttccgt
     3661 ttgtcctttc cttacttgtg gtacgtttta tttctgttta tttcttctgt accatcttgg
     3721 ttcgtgccat tgtcaaactc tatcatcagt ttgcaactgg aggaaaagca tagatgggtc
     3781 tggaactacg agcgattcag tccccaatct tctctgagcc gtttgatttt acttttcatg
     3841 cgcaagcctt taccttgtta gttgggagca gtggttcagg aaaatccagc ctttttcaag
     3901 tcattgccca agtcagttct cttccctata gtggtcaagt cctgatagat gggagcgagg
     3961 tcagtcagct ttctatcatc gcacgtgtcc agaaggttgg cattctcttt caaaatccca
     4021 atcatcaatt taccatggag aacttgtttg aggagctgat tttcaccttg gagaatatcg
     4081 gctatcacct tcaggaaatt gattctaaaa tagcagaggt tgtccagcaa tgtcgttgcg
     4141 aggcaatctt acaccgtccc attcatcacc tatcaggtgg ggaaaagcaa aaagcagcgc
     4201 tggctgtcct ctttgccatg aatcctaggg tctatctctt ggatgagccc ttcgcttcta
     4261 ttgaccgcaa gagcagaatc gagatattgg agattctaaa agagttggct cttgatggga
     4321 agacagttat tttgtgcgac catgatttat ctgactataa agcctatatc gaccatatgg
     4381 ttgagctaag agacggaaaa ctaagggaag tgtttcaaat cccttcctat gagatgacac
     4441 aggttgcttc aaaggaagtt gcttctagcc cggaactatt ccatatgaac cgtgtgactg
     4501 gtgagcttgg taatcgcccc ctcttttcaa ttgctgattt cacattctat caagggattt
     4561 cctgtatcct gggtgacaat ggtgtcggga aatcaaccct ctttcgctct attcttcaat
     4621 ttcaaaagta taaggggcgc attgcatgga aggggacagt cctgaaaaag aaaaagagtt
     4681 tgtaccgtga tctgacgggt gttgttcagg aagctgagaa gcagtttatc cgagtcagtc
     4741 tgcgagagga gcttcaatta gatggacctg attctgaaag aaatcagcgg atttttcaag
     4801 ctttacgata ttttgatttg gagcaggcag tcgataagag tccctatcaa ttaagtggtg
     4861 gtcagcaaaa aattcttcag ctcctgacca tcttgaccag taaggcttcc gtgatcttgc
     4921 tagatgaacc ttttgcaggt ttggatgata gagcctgcca ttatttttgc aagtggattg
     4981 tggaggagag gaatcaagga agaagttttc tgctcattag tcatcgttta gaccctttga
     5041 tttctgtggt tgattattgg attgagatga ctagtcaggg gctcagtcat gtgaaagaag
     5101 tgaccattac caaaccactt acatctcaga gtagcaatac ccaaggggag gtgagatagt
     5161 atggtcaaag tagcaaccca gacaccgatt atcagtctct tcttgctgat tttatccttg
     5221 gaaacatctt tcattccttc gattgctctg aatctttcgg tagtcgcatt ttgtattctc
     5281 tttatgctct attaccgtcg atttaaaatg ttagcttgga tgatcatact tgccatttta
     5341 ccatctttgg ccaactactg ggcagttcag ttacacggag atgcttcaca ggcagtcatg
     5401 cttggaacga gggcctttgt gacagtttgt atcggccttg tctttgtttc ctctatttca
     5461 ctaaaagagc ttctcttgta cttggctcaa aaggggctat cacgctcttg gtcctatgcc
     5521 ttgattgtgg tattcaattc ctttcctctc attcagcaag aaatcaagtc cctcaaagaa
     5581 gcttgcctat tacgtggtca agaactgcat ttttggtcgc ccttgattta cagtaaggtt
     5641 ctgatgacag tctttaggtg gcgccatctt tacctgagag ctctgtctgc tcatggatat
     5701 gacgaacatg cacagttgaa gaatagctat cggacttttt atattcctaa aaaaacaaaa
     5761 ttaatctacc tgcttttctt tttattgctt caaaccagtc tatttttata aaggagttat
     5821 tatggaattt acagatattg cgatggaatt atccaagaaa gcttggcagg cttcctttca
     5881 tcatcccttt attttacaat tacaagaggg gaatttagaa cctgccattt tccgctatta
     5941 cctgattcag gatgcctact atctgaaggc cttctcagaa atctatcatc ttttggctga
     6001 taagacttca aaccaagaga tgaaaagact cttgaaacaa aatgctcagg gtttagtgga
     6061 gggtgagtta tttattcgcc aacaattttt caaggaaatg gaaatcagcg accaggaaat
     6121 ggagcaacat ccaattgctc caacctgtta tcattacatt tctcatattt ataggcaatt
     6181 tgcagaaccg aacttagcca tcgcttttgc aagcttgctt ccttgtcctt ggttatacca
     6241 tgatataggc aaatcactta atcttaaacc atcaccaaat cctctctatc aacaatggat
     6301 tgaaacttat attacggatg agttagagca acagatcaga gaggagggag cactggtcaa
     6361 tcagctctat cgagaaagtg acgagacaga caagcaaaaa atgctagatg ccttctacat
     6421 cagtgttcat atggaagcca agttttggga aatggcctac caacaccaga catggaagag
     6481 cgatttacag tctttagaaa aaggagaaga atagaagcat gagaaagcac caattacaag
     6541 ttcacaaatt aaccatttta tctatgatga ttgcccttga tgtagtcctt acacctatct
     6601 ttcgaattga gggaatggca ccgatgtcca gtgtagtcaa tattctagcg ggaatcatga
     6661 tgggacctgt ttatgccttg gccatggcta cagtcacagc ctttatccgt atgacgactc
     6721 aagggattcc gcctttagct ctcacaggag cgacttttgg agcccttctg gcaggtctct
     6781 tttataagta cggtcgaaaa tttcactatt ctgctctagg agagattttg ggaacaggta
     6841 ttattggttc tattgtttcc tatcctgtta tggtgctctt tacaggatca gctgctaagc
     6901 ttagctggtt tatctacacg cctcgatttt tcggagcaac cttgattggt acagcgattt
     6961 cctttattgc ctttcgattt ttaatcaagc aggaattctt taaaaaagtg cagggatatt
     7021 tctttagtga aaggatagac tgatgcagga atttacaaat ccctttccta tagactctag
     7081 ttccctcatt cactgtatga ccaatgagat ttcttgtgag atgctggcaa atgggatttt
     7141 ggctctggga tgcaaacctg tcatggcaga tgatccccgt gaggttcttg attttactaa
     7201 gcaaagtcag gctctcttca tcaatttggg gcatttgtca gctgagaagg agaaagcaat
     7261 ccgtatagca gcttcttatg cagctcaagt ttgtctccca atggtagtag atgcggttgg
     7321 cgtaacgact tcatccattc gtaagagctt agttaaagac cttttagact atagacctac
     7381 ggtccttaaa ggaaacatgt cagaaattcg aagtcttgtt ggattaaagc accacggcgt
     7441 tggggtcgat gcgagtgcta aagatcaaga aacggaggat ttgcttcaag tcttgaaaga
     7501 ctggtgtcag acctatcctg gtatgtcatt cttagttact ggccccaagg acctcatcgt
     7561 ttcggagaat caagttgctg tactggagaa tggctgtact gaattagact ggataacagg
     7621 gacaggagat ttggttggag ccttaacagc cgtttttctc agccaaggaa agactggttt
     7681 tgaagcttct tgcttagcag tctcttatct caatatcgct gctgagaaaa tagttgttca
     7741 aggaatggga ttggaagaat ttcgttacca agtactcaat cagctttcgc tcctaagaag
     7801 agatgaaaat tggctagata ccatcaaagg agaggcttat gaatagagaa gcacttagac
     7861 tatatctggt aaccaatcgc tatcaagatt ccgtggaaag ctttcttgca aaagttgaga
     7921 cggcctgccg ttcaggggtt accatagtcc aattgcgaga aaaaaatctc acaaccaatc
     7981 aatattatca actggcaaaa caagtcaagg aaataacaga tgcttatcag gtacccttga
     8041 taatcgatga tcggttggat gtttgtcttg cggttgatgc tgctggtctg catattggag
     8101 atgacgaact cccagtttcg gttgcccgac aagtcttggg tcctgaaaaa atcctcggtg
     8161 tcaccgctaa aacggttaaa agagctctgg aagcggagaa atcaggtgca gattacttgg
     8221 ggacaggagc catttttccg acaaccacca aggaaaatgc acccatcacc ctgatttcaa
     8281 ccttgaaaac aatttgccaa acggttgcca ttccaatagt tgctattggc ggcttgactt
     8341 cggagaatat tgatcagctt atgggcactg gcatagctgg tgtagctgtt gtacgtgatt
     8401 tgatgcaagc agaagatatt gaggcaaaaa cgcaagcctt tttaacaaag ttgcatgaca
     8461 ttctttccta attaatactc aatgaaaatc aaagagtaaa ctagaaagct agtcgcaggt
     8521 tgctcaaagt actgccttga ggttttagat aagactgacg aagtcagctc aaaacagtgt
     8581 tttgaagttg cagatggaag ctggcgtagt ttgaagagat tttcgaagag tataaaaact
     8641 ctctaattcc cttaaagtgg gaactagaga gtttttttct taatctttaa aagttgtatg
     8701 gttgactgga ccagaaccat gaccgagttg aggggcatct tgaatagctt ttgtgataaa
     8761 ggccttggcc ttatcaactg cctggtaaag actcttgccc ttggctagtt cagcagtaat
     8821 cactgcagca aaggtacatc cagtaccatg ggtgtgacag gtttgaattc gtgggctttc
     8881 ccagacaaat tgttcattct tggtaaagag gaaatcttta gcaccacctt tgagatgtcc
     8941 gcctttgata accacagact gaggaccaaa ttcttttaaa atcaggcgac cagcacgctg
     9001 catgtcttcg gggtcatgga ttgaaaaacc aacaatctct tctgcttcag gaagatttgg
     9061 cgtaataata gttgctagag gtagtaagtt tgttttgaga tagtctctag catttgagtc
     9121 aatcaaggca tctccacttg tagcaaccat aacaggatca aggacatagg gacaatccag
     9181 ttttttaaga tagggttgga tgatttccat gatttcagta gtagccaaca ttccagtttt
     9241 tacagcctga ggtggaatat cagaaaagac actctccaat tgggctttca acatttgagg
     9301 agaaacgtgc tcgattagct gaacacctct ggtattttga gcgacaagac tggttacaac
     9361 agccattcca tagacatctc tcgcttggaa tgactttaaa tctgccataa tgccagcacc
     9421 accactaggg tcagtccctg caatggtcaa agcaacgggt aaataagtca tctaaaacct
     9481 cccaaccaac tgaagtttgt attcttaaga acaagctagt ctgttttaga aaggcaatag
     9541 aagactgcta gtccccatta tcatttccac ttccatcagc tagcattacc tagattgagt
     9601 cagagggatc taacagtcgt taatctcagc tttacgcacc cctagtggtt gttttctttt
     9661 ttctttagta tagcatattt ttattgttta tatagtaata tttgactgca aatcgttttt
     9721 caaatgatta aaattctatt tttcgaagaa aatattattt ctataattga caaatgtaga
     9781 ttttgggagt atactgttga gtgtaattga caaatgtaga ttttggaggt atggtaatgc
     9841 agatttcaga tgcagaatgg caggtcatga agattatttg gatgcagggg gagcagacca
     9901 gtacagattt aattagggtt ttggcagagc ggtttgactg gtccaagtcc acggttcaga
     9961 cacttttggc tcgtctagtt gagaaagagt gtttgactcg gaaaaaagaa ggcaagttct
    10021 ttgtttattc agcgctttta actctagacc aaagtcggga tttacttgtc caagatatca
    10081 aagacaaggt ttgttcccgt aggattagga atttgttggc tgacttgatt gtagaatgtg
    10141 aatttactca gactgacttg gaagacttgg aagctgtgat ttcagagaag aaatcaagtg
    10201 ctgtaacaga agtaagatgt aattgtatgt aaaggagacg ccatgttaaa tagtattgta
    10261 accattattt gtattgccct tatcgcgttt atcttgtttt ggtttttcaa aaagcctgaa
    10321 aaatctggac aaaaagccca gcaaaaaaac ggataccaag agattcgagt ggaagtcatg
    10381 ggaggctata ctcctgagtt gattgtcctc aagaaatcag tgccagcccg cattgtcttt
    10441 gaccgcaagg atccttcacc atgtctggat caaattgttt ttccagattt tggtgtacat
    10501 gcgaacctgc caatggggga agagtatgta gtggaaatca tgcctgaaca ggctggagag
    10561 tttggctttg cttgtggtat gaacatgatg cacggcaaga tgattgtaga gtaggtggag
    10621 actatgacag aaattgtgaa agcaagctta gaaaatggca ttcaaaaaat ccgtatccga
    10681 gctgaaaaag gctatcatcc agcccatatc cagcttcaaa agggaattcc agctgagatt
    10741 acctttcatc gtgctactcc ttcaaactgt tataaggaaa ttctgtttga agaagaaggt
    10801 atcttggaac caatcggcgt agatgaggag aaagtcattc gttttacgcc tcaagaatta
    10861 gggcgacatg aattttcttg tggcatgaag atgcaaaagg gaagctatac agtcgttgag
    10921 aagactcgaa aatctctatc tctcctgcaa cgtttttgga ttactagtat ctttactgtg
    10981 cctcttgtga ttctcatgat tgggatgttg acaggtagca ttagtcatca agtcatgcat
    11041 tggggaacct ttttagcaac aacgcctatt atgttagttg caggtaagcc atatatccag
    11101 agtgcttggg ccagttttaa aaagcacaat gccaacatgg ataccttggt tgcgctggga
    11161 actctagtgg cttatttcta tagcctagtt gctctctttg ctggtctccc tgtttacttc
    11221 gaaagtgctg gatttatcct ctttttcgtt cttttgggag cagtttttga ggaaaaaatg
    11281 agaaaaaata cgtcccaagc tgtggagaaa ttactggact tgcaagctaa aaccgcagaa
    11341 gtcttgagtg atgatagtta tgtccaagtt cctttggaac aagtcaaggt aggcgacctg
    11401 attcgagtgc gtcccggtga aaagattgct gttgatggtg tcgtagtaga aggtgtctct
    11461 agtattgacg aatccatggt gacaggtgag agtctgcctg tggacaagac agttggagat
    11521 actgtcattg gctcaaccat caatcatagt ggaacgcttg tctttagagc agaaaaagtt
    11581 ggctcagaga ctgttttggc tcagattgtg gattttgtga agaaagctca gacaagtcgt
    11641 gcgccgattc aggacttgac ggataagatt tcagggattt ttgtcccagt agttgtcatt
    11701 ttaggaatca tgaccttttg ggtttggttc gtcttgctca gggatagtgt ggttgtgctt
    11761 ggagctagct ttgtgtcctc tcttctctac ggagtggcgg ttttgattat cgcctgtcct
    11821 tgtgccttgg gacttgcaac accgacagcc cttatggtgg ggacaggacg tagtgccaag
    11881 atgggggttc tcctcaaaaa tggaactgtc ttacaggaaa tccagaaagt tcaaactctt
    11941 gtctttgata agaccgggac tttgacggaa gggaaacctg tggtaacaga tgtcatcggc
    12001 gacgaagtag aagtgtttgg gttggcagcc tccttggaag atgcttctca acacccactg
    12061 gctgaggcta tcgttaagcg agcgagtgaa gctggacttg agtttcaaac tgttgaaaat
    12121 tttcagacct tgcacgggaa aggtgtttca gggcgaatca atggaaaaca agttttactt
    12181 ggaaatgcta aaatgctgga tggcatggat atttctaata cttatcaaga taaactagaa
    12241 gaactagaaa aagaagctaa gacagttgtg ttcttagctg ttgacaatga aatcaaaggc
    12301 ttgcttgctt tgcaagatat tcctaaggaa aatgctaagc tagtcatcag tcagctaaaa
    12361 aaacgtggtc tccgaacagt catgctgaca ggagacaatg ctggtgtggc gcgtgctatt
    12421 gcagatcaaa tcggaattga agaggtcatt gcaggcgtct tgccagaaga aaaagcccat
    12481 gaaatccata aactgcaaca gtcagggaaa gtagcctttg ttggggacgg tatcaatgac
    12541 gctcctgccc ttagtgtagc agatgtgggg attgctatgg gagctggaac agatatcgcc
    12601 atcgaatcag cagatttggt gttgacaacc aataatcttt taggagtggt tcgtgccttt
    12661 gatatgagta agaaaacctt tcatcgaatt ctactcaatc ttttctgggc ttttatctac
    12721 aatgttgttg gaattccgat tgcagcagga gtcttttcag gtgttggact ggctctcaac
    12781 ccagaattgg caggtctagc catggccttt agttctgtat ctgttctgac cagttccctt
    12841 ctgttaaatt ttagtaaaat agactaaaag taatgactag atttctttgt tataaaacag
    12901 aaatgacaac actttcaaaa ctgtattata atagagaata gagagttttg agcagatttt
    12961 tagaaaagtc agcataaata tgatacagtg gaatagtaaa aatttggaga acgtttccaa
    13021 ttctatgtaa tcgtattctc caagtctaaa aaaattgaag gagagttatc attatgactc
    13081 aagggaaaat tactgcatct gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata
    13141 caatctacgg tatcccatca ggaacactca gctcattgat ggatgctttg gctgaagaca
    13201 aagatatccg tttcttacaa gttcgccacg aagagacagg tgctcttgca gcggttatgc
    13261 aagctaaatt cggcggctca atcggggttg cagttggttc aggtggtcca ggtgcgactc
    13321 acttgattaa cggtgtttac gatgcagcta tggataacac tccattccta gcgatccttg
    13381 gatcacgtcc agttaacgaa ttgaacatgg atgctttcca agagcttaac caaaacccaa
    13441 tgtacaacgg tatcgctgtt tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag
    13501 taattgacga agcctgccgt gctgcagttt ctaaaaaagg tccagctgtt gttgaaattc
    13561 cagtaaactt cggtttccaa gaaatcgatg aaaactcata ctacggttca ggttcatacg
    13621 aacgctcatt catcgctcct gctttgaacg aagttgaaat cgacaaagct gttgaaatct
    13681 tgaacaatgc tgaacgccca gttatctatg ctggatttgg tggtgttaaa gctggtgaag
    13741 tgattactga attgtcacgt aaaatcaaag caccaatcat cacaactggt aaaaactttg
    13801 aagctttcga atggaactat gaaggtttga caggttctgc ttaccgtgtt ggttggaaac
    13861 cagccaacga agtggtcttt gaagcagaca cagttctttt ccttggttca aacttcccat
    13921 ttgctgaagt ttacgaagca ttcaagaaca ctgaaaaatt catccaagtc gatatcgacc
    13981 cttacaaact tggtaaacgt catgcccttg acgcttcaat ccttggtgat gctggtcaag
    14041 cagctaaagc tatccttgac aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa
    14101 acgttaagaa caaccaaaac tggcgtgatt acatgaacaa actcgaaggt aaaactgagg
    14161 gtgaattgca attgtatcaa gtttacaatg caatcaacaa acatgctgat caagacgcta
    14221 tctactcaat cgacgtaggt aacactactc aaacatctac tcgtcacctt cacatgacac
    14281 ctaagaacat gtggcgtaca tctccactct ttgcgacaat gggtattgcc cttcctggtg
    14341 gtatcgctgc taagaaagac aatccagatc gccaagtatg gaacatcatg ggtgacggag
    14401 cattcaacat gtgctaccca gacgttatca caaacgttca atacgacctt ccagttatca
    14461 accttgtctt ctcaaatgct gagtacggct tcatcaagaa caaatacgaa gatacaaaca
    14521 aacacttgtt tggtgtagac ttcacaaacg ctgactacgc taaaattgcg gaagctcaag
    14581 gagctgttgg attcacagtt gaccgtatcg aagacatcga tgcagttgtt gcagaagctg
    14641 ttaaattgaa caaagaaggt aaaactgttg tcatcgatgc tcgcatcact caacaccgtc
    14701 cacttccagt agaagtactt gaattggatc caaaacttca ctcagaagaa gctatcaaag
    14761 ccttcaagga aaaatacgaa gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag
    14821 aaggattgca atcacgcgca attaaataat tcctctcgcc gaaaatcaaa tatgaaactt
    14881 gtagacttta ttctttgatt ttcatagagc agaactttaa aagatcggag caatccggtc
    14941 tttttatgga ggagagtaaa tgaatttaaa tcaattagat attatcgttt caaatgttcc
    15001 ccaagtctgt gctgacttgg agcatatctt ggataaaaag gcagattatg ctgatgatgg
    15061 ttttgctcag tttacgattg gtagtcactg ccttatgttg tcacaaaatc atttggttcc
    15121 tttggaaaac tttcagtcag gaatcattat tcatatcgag gttgaggatg tagaccagaa
    15181 ctacaaacgg ttgaacgagc ttggtataaa ggttttacac ggaccaactg taaccgattg
    15241 gggaacagag tccttattag ttcaaggacc tgctggtcta gtgcttgatt tttatcgtat
    15301 gaaataggtc gcgtagtcat ttgacgttaa ccttagctat ttttaaaata gaaattgaga
    15361 atatgaacta ttgaaagatc ggagcaatcc ggtcttttta tataaagaaa ttgcatataa
    15421 tactcatctg ctttcaaaaa gcattctagt ccatctccga ttaacgatgg actttatcac
    15481 ctccttctcc aatccttgta tgacatcttg gagttgattc atgacatctt ccaaagttcg
    15541 aaaggcttta ttcttaaatc cacgtttacg aatctctttc cacacttggt caatggggtt
    15601 catctctggt gtgtatggag gaataaatgc aaagccaata ttagtcggaa tctttaaggt
    15661 acttgattta tgccatatag cattgtctat aacgagtaaa aaataatcat atggataagc
    15721 ttgtgaaagc tcttctaaaa aggcgttcat ccactcagta ttacatctac cagctattaa
    15781 gaaaaatgat tcgcctgtat gggcatcaac agctccataa caatagcgaa attctcgtat
    15841 atagtgacta tggacatgtg gacctactcc tattggagac caacaagatc ccagtttact
    15901 gattctaccg aaaccagctt catcttggta catcaagcga accttatgaa aaggtctact
    15961 ggttttaaag cgctttcttg tcttcttgaa ttgagatttt atttttagat gcgacaatgg
    16021 tttgagcgtc tgctttctta ggatgttctg gacgtggcgt aatatttcgc caaccatggc
    16081 gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg acctaactcc tttttataag
    16141 cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc tgtagccttc aaatggcgag
    16201 caagaaaggc tttctcttcc tcaactgtca tatatgcatg gttacgacca ccacgtgttt
    16261 cttgaaggag agagtcgagt ccgaactcct catatttttt acatttcgcc aaatcgttgt
    16321 ttgattacag tctaaaagct ctataatctc tttataagat ttgcccatca gacgaaatag
    16381 aacgatttga aggcgtttat gatatttagc tgtacgagag tcttttaaaa gtgttttgat
    16441 ggtttggatt tcttctttag tcaatttcat atcactatta tataatgctt tttgatttta
    16501 gtttagtatt atcgtagaaa atggactcgg tactatggat acaccggatg agaatggtta
    16561 tgtagcagat gactatcgga ttacttactt agaggcccac atcaaggcca tgcgagatgc
    16621 catttaccaa gacggggttg acttgcttgg ttatacgact tggggctgta tcgatccggt
    16681 ttcagctggc acaggggaaa tgaataagcg ctatggcttt atctatgtag atcgagataa
    16741 tgtaggaaac ggagttctca aacgtagcaa gaagaagtcc ttctattggt acaaggatgt
    16801 cattgatagc aatggtgcaa gcatcgagta gagcacattt gtttactatt tttataaaat
    16861 cttctccctg ctttataaga tgtaaaaaag agttcaaatg gaactctttt tttgctataa
    16921 tgaggggaga aaaatcagac aggagattaa gatgtcagaa ccattatttt tacaatcagt
    16981 tatgcaagaa aaaatctggg gtggtaccaa gctacgtgat gagtttggct acgacatccc
    17041 aagtgaaaaa gtcggagaat actgggctat ctcagctcat ctaaatggtg tttctaaagt
    17101 tgccaatggt cgttacgagg gaacagatct tgctactttg tatgcggaac accgtgaatt
    17161 atttggcaat cgtccagaac ctgtatttcc acttttgacc aagatcctcg atgccaacga
    17221 ctggctcagt gtccaagttc acccagacga tgcttatgga ctcgagcatg aaggcgaact
    17281 cggaaaaaca gaatgctggt acatcatcgc tgcggatgaa ggttcagaga ttatctacgg
    17341 tcacaatgcc aagtcaaaag aagaactccg ccagcaaatc gaggacaaga actgggatac
    17401 cttgcttacc aaagtccctg tcaaggctgg agatttcttc tatgtaccaa gtggcaccat
    17461 gcatgctatc ggtactggca tcttgattct tgaaacccaa cagtctagtg ataccactta
    17521 ccgtgtctat gactttgacc gtaaggatga caaaggcaac ttgcgtgaac ttcatcttga
    17581 aaaatccatc gatgtcttga acattggtga gccagcaaat agccgtcctg taactgttaa
    17641 agcagatgat ttgcgttcca ctctccttgt atctaatgat ttcttcgcag tttacaagtg
    17701 ggaaattact ggaaaagttg actttgaaaa gacagctgac tacagcttat tgagtgtctt
    17761 agctggtcaa ggtcagctaa ctgttgacgg gaaaaattat ccaattcaaa aagggagaca
    17821 ctttatccta ccaagtgatg ttgaagcttg gactctggaa gggcaaggtt tggaattgat
    17881 tgttagccat ccataaaaag aaagggcctg agttcactac tcaagtcctt tttgattaca
    17941 taaattgggc aatgaagacc acaatgatga tgattggaat gacgaaacga agaaacgaag
    18001 aaggaagagc cagacttgga acagtccttg tttccatgct ctttcatcga gatggagttc
    18061 ctccattgca agagcctttt taaagatata gcctgtaaaa agtgaaaggt agagagctcc
    18121 aaatggcatg aggagattgg aagccaagaa gtccatagcg tcaaagaaga tcttaccaaa
    18181 aatgtgaaca tccgccatga caccgtaaga tagggctgaa ggaatgccaa agacaaaggt
    18241 caaaattcct aaaataacac tccatttggc acgtttgctg ttatcctggt tggtgatatt
    18301 gcccacattg atctccagca tcacgacaga agaagtgaca gtcgcaaaga ggaagaggac
    18361 gtagaaaatg gttccaaaag gcatcttgtc aaagagttga ggtaagacga taaagagcag
    18421 gggcatcatg actgtaaccc ctagtgagag ggcaaagaaa gattgtccca gagcatagag
    18481 gagaccagcg ctagtcagtt ttgaaaagtc tggtttgagg aagtaaagaa ccccttccat
    18541 ggcatttggc aaactgagag agcgaccgat gataacaaca aagatgataa agagcagggg
    18601 catcatgact ttcgaagctc tttcaatccc tttttgaacc ccacgtgata caataaagat
    18661 atttaataag ataaaggctg cttgagctac tagggcaatg gctggatttg aaatgattga
    18721 agtaaataac tgagcataat cacccgttcc accaagttgg aacaatttcc caaactcaat
    18781 acctagatag actagaatcc atcctccgat aacactgtaa aaagataaga ggataaagag
    18841 ggcaaaggcg ccaatccaac cgataaagtt gtacttgtta ttcttgccca gttttccaaa
    18901 ggttttgata gcggaaacgc cagcactacg gccaagggca aactcagcca gcaggagagg
    18961 gaaaccgatt aaaatagtgg aaatgagaaa gattagtaaa aagcctccac cgccattagc
    19021 agcagtcatg tagggaaact tccaaacgga accaagcccg atgaccgagc cagcagatgc
    19081 tagaataaag ccaagtttcg acccccattg tgatttttct gacatagtta aactctgatc
    19141 tcctttttca aaataagaaa aagctacttg agttgtcaaa tagccttctg tttgattgat
    19201 agacttgact atcctattat gttttctaag gtctgtcaag aaaaccattt tcaagttttc
    19261 acacctttct caaaaacgct taactctgac ctaaggggag gggttatact atcattgtaa
    19321 ggaggaaatc atgtaccata taaaagaagc tgcgcagctt tcaggtgtct ctgtcaagac
    19381 cctgccctgc atcactatga caagatagga ctcttggtcc ccttaaagtc ggaaaacggc
    19441 tatcgaacct atagtcaaga ggatttggaa cgccttcagg tcattcttta ctacaaatat
    19501 ctaggctttt ctttagagaa aatagcagag ctgttaaagg aagaaaggac agatttattg
    19561 ccccatttaa ctaggcagtt ggactatcta actcgcgaaa ggcaacatct ggataccttg
    19621 atttccacct tgcaaaaaac tattcaagaa caaaaaggag aaagaaaaat gaccattgag
    19681 gaaaaattca cgggatttag ctatcaagac aatcaaaaat accaccaaga agcggtagag
    19741 aaatatggtc aagaagtcat gggacaagcg cttgaacgcc aaaaaggtca cgaaaacgag
    19801 gctacggccg cctttaacca agtctttcaa actttggcac aaaatcttca agctggttta
    19861 cctgcaacag caaccgaaaa ccaagagcaa gcagccaagc tcttgcaagc cattcgcact
    19921 tatggatttg actgctctat tgaggtattc ggtcatatcg gtaaaggtta cgtctacaac
    19981 ccagagttta aggaaaacat tgacaagttt ggttctggaa cagcccagta cacgtcagat
    20041 gccattgcgg cttacgttca gacaaatgca gaaaaaatag gctaggaatt tcctagccta
    20101 ttttttactt caaatcataa agccagtcgt caccgttttt gtagtaaaag aattcactga
    20161 gatcttcttc tagaaacaca cgaagcatat cagacatatc atcggttgca agttttagat
    20221 gagaaagatt ttcaaagtcc tcccactaaa ctttcccttc gtctgaagac tggagttcac
    20281 cagtaaagtg ttctgtcttg taaaaaagga cgacataacg ataatccttg tcgtcatacc
    20341 agtttttgat accacagagt tggggtttgg aaatgatcag accagtttct tctttcactt
    20401 cacgaatgac agcatcgaca aaggattcgc cacgttcaac atgaccacca ggaaaagtaa
    20461 tgccagacca gtcgggacta actcggtctt ggaccaggac cttatctccg tttttaatca
    20521 tacacatgtt aacaaattcg actgcctctc ttctgttcat tcttcacaac ctttaatctt
    20581 taatcataat gcagacttcc cgccacccag ccggtacaga gggcagaagt gatgttaaag
    20641 ccacccgtgt gggcattgat atccataact tcgcctgcaa agtggaggcc aggtaccagc
    20701 ttactttcaa gggttttagg attgatttcc ttgagactga ctccaccctt ggtaacaaag
    20761 gactttgcaa gggacatttt tccagttaca ggaattttaa gttctttaat ggactggaca
    20821 agttgttctc gttccttttc agtcagctgt ttgacttttt caggatatcc ttgtacaaaa
    20881 aattcggcca agcgttctgg taacaaggtt tttaaagcgt ttttcaagga tttttcccga
    20941 ttttcttcta gaaatgtaac caagtccttc tcagaaagtt gaggcaaaac atcgagtgag
    21001 agaacctccc cacctttgac aaagctagac atgcgtaggg cagcaggacc tgacaaacca
    21061 aagtgggtaa agagtaaatc atgagtgatg acatgcttac cataacttag ggtcacatcg
    21121 tccaaagaaa tcccttgtaa ggctttatgt ggaaaatctg ttaataaagg actttcagca
    21181 gcctcaagct cggtgatggt atgcttaaaa tggcgagcaa tctcgtgacc aaaaccagtc
    21241 gaaccagtcg aaggataaga cttaccacct gttgtgacaa tgagtttctc acaagtgaag
    21301 gtttgatccg ctgacttaag gacaaactgg tcatctactt ttttaacaga aacgatttct
    21361 atttgagtag caacttgacc acctagttca gtgattttct tttccaaagc ttcgataata
    21421 gtccgagact tgtcactggc tggaaagacg cgtccgtggt cttcgacctt aagtttaaca
    21481 ccattttctg taaaaaagtt gatgatgtca tgattatcga actgggagaa aacactgtaa
    21541 agaaagcgtc cgtttccagg aattccagct agcaggttgt ctaagctacc attgttggtc
    21601 acattgcaac gtcccccacc agtcccagct aatttttttc caagtttccg atttttttcg
    21661 atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg tagccatcat accagcaggt
    21721 cccccaccga tgacaatagt atcaaaatgt ttcatagctc tattgtacca caaaaaaaca
    21781 agagatgatg gtcacctctt gtcaagaatg caattaatca atttcatagc ccatcagcaa
    21841 accgccctct tctgcataga aactgcagag accagaggtt ggtagaattt taatatccgc
    21901 ttgtgggaag gtttcacgga ttcgctctga gagctgttga caacattttt cgttattgcg
    21961 ttgggccatg acaatacggc caccagcata tccagctttt actaactcat cataggcagc
    22021 ttgaactgat ttctttgatc cccttgcttt ttgtagcaat tcgagagttc cagtttcact
    22081 agcttttccg accatacgaa tgttgagaag gccaacgacc gtaccgataa gcttgctcaa
    22141 acggccgttc ttcaccaagt tatcgacttt ggctaggaca aagagcaact tagttttttc
    22201 ttgataggcg gtgatagctt caaccacttc ttcaaaagac aagccctggt caatcaagtc
    22261 attcaatttt tctacgagta ggtcaacttc accaccagca gataaactat caatcacatg
    22321 aatcttagtg tcaggatggt cttccagata aatattcttt gctagttgag cactattgtg
    22381 actgccagaa agggtacctg tgatggttac taggaaaatg tttttggcac cttcaaatgc
    22441 tcgcaaatag tcatctgggc ttggacaagc cgattttgaa gcttctgcag ttgcatacat
    22501 ggtttccatc atttggtcaa tatcgagact ggcgtcatca acaaagacct gatcagctac
    22561 ttgaatggtt aaggggacac ttacaaaggt tgtgttaata gctggtgttg gcagttgacg
    22621 ataatcacaa ccagagtcag caataatctt ccaagtcata gaaattctcc atctttgtca
    22681 gttatacatt gacaaaggtt ctgtcttttt ttacaattat atcatgaaaa cccttgaaac
    22741 aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat gttatgtctg aacgtagaat
    22801 ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa gaatccaatt tattaaccag
    22861 agaagccatt gaaacagccc tcttgcaact cttggaaaaa aaggaactga ccaagattag
    22921 tatttctgaa ttggtcaaac gtgcaggtgt ttcgcgtgcg gccttttatc gcaattatga
    22981 ctccaaagag gaaattttag agagcgtctt taaacgaact gtccacaata ttatggaaca
    23041 gctgcatcat tacgatttaa agacagacct ttatttggtt tgggttcacc ttttccggga
    23101 ggccagaaag gaagctagag tgattcaatt ggccttggat taccatctgg aaaaaatctt
    23161 tgtccaagcc atgcaggaat ttctagaaaa ataccatggg aaatcaaaag gtgtcagctc
    23221 ttatcttcat tccttctgga gctcagccat cgtctctgtc cttctaaaat ggatcaagga
    23281 tggcatgaag gtaccagctg aaaagattgc agatttaggt ttaccatttt ttaaaaaata
    23341 gagaaaaagg agaaaagaga tgactgaaaa aagactggcc tgggatgagt attttgcagc
    23401 ccaagcccta ctaattgcga atcgttccac ttgtaaacgt gctaaagtgg gcgcgattct
    23461 ggtaaaagat aataaggtta tttccactgg ttacaatggt tcggtgtcag ggactgagca
    23521 ttgtattgat cacgaatgtt tggtcattga aggccactgt gttcgcaccc ttcacgctga
    23581 ggtcaatgct atccttcaag gtgctgagcg tggtgttccc aaaggcttta cagcctatgt
    23641 aacccatttt ccttgtctca actgtacaaa acaattgctt caggtcggct gcaagcgtgt
    23701 ggtttatatc aaccagtacc gaatggacga ctacgcccaa tacctttatc aagaaaaagg
    23761 gacagaattg acccatttac cacttgagac agtacagaca gctcttaaag aggctgatct
    23821 aatgtaaaaa ttatcaaaaa taaatggttt agaaagattt ttaaaccgtt ttttggtata
    23881 ataagaagaa taaattgaaa gaaggaattc caaaaatggg aaaaattgaa gttattaatc
    23941 acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac agatacttct acaaaagctt
    24001 ttcgtgagct agtagatgag attgcaatgt tgatggggta tgaagtactt cgtgatcttc
    24061 cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac agttcaaaaa caattggcag
    24121 gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat cgggatggtt gatggtctct
    24181 tgagcttggt tccagctgct aaagttggcc acatcggtat gtaccgtgat gaagaaacac
    24241 ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat tgaccaacgt caaatttttg
    24301 tagtagaccc aatgttggca acaggtggct cagcaatctt ggctgttgat tctcttaaaa
    24361 aacgtggcgc atcaaatatc aaatttgtct gccttgtatc tgctccagag ggtgtaaaag
    24421 cccttcaaga agctcatcca gatgtagaaa tctttacagc agccttggat gaacgtttga
    24481 acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg agaccgcttg ttcggtacaa
    24541 aataagatcg aaaagaagaa tgacttggaa aagtatttcc agtcacgaaa ggaggttggg
    24601 tttttgtttc tgtctaatga aagcagagca aaaatttgac cttttttgac caagatatta
    24661 taatagtctt atcttcagtc atttgaccaa caaaattaaa actcaaaagg agaaatgaat
    24721 gattcctgta gttattgaac aaacaagccg tggagaacgt tcttacgata tttactcacg
    24781 tcttctcaaa gaccgcatca ttatgctgac aggtccggtt gaagacaata tggctaactc
    24841 tgttattgcc caattgcttt tcttggatgc ccaagatagt acaaaagata tttaccttta
    24901 tgtcaataca ccaggtggtt ctgtttcagc tggtttggca atcgtagata ccatgaactt
    24961 tatcaaggca gatgtccaaa ccattgttat gggaatggct gcatctatgg ggactgtcat
    25021 cgcatcaagt ggggcaaaag gcaaacgttt catgcttcca aatgctgaat acatgattca
    25081 ccaaccaatg ggcggtacag gtggtggtac ccaacaaact gatatggcta tcgctgcaga
    25141 acacttgctc aaaactcgta ataccttgga aaaaatcttg gctgaaaatt caggtcagtc
    25201 aatggaaaaa gtccatgcag atgcagaacg tgataactgg atgagcgccc aggaaacact
    25261 tgaatatggc tttattgatg aaattatggc caacaattca ttgaactaat gatgatagaa
    25321 ggcaaactcg actgggtttg ctttttttgg tataataggg agagatttct tagaaagagg
    25381 atttatcatg tttgaaaaag tcaatcgctc tggcttgatt atctatcttt actataatcg
    25441 tgatgccaaa aaactgcagg attatggaga tattacctat cattccaaga aacatcgtta
    25501 cttacaactc tatgttccaa ctcaagaagt ggagcaattg gtcggacgct tgagcaagga
    25561 aaaatttatt aaaaaagtta gggtttgtca tatccaagag ttggaaacac cctttgtggg
    25621 caatctttat cgataggaaa acgttatcat cgaaaaaagc tcaaggaaaa cattgacaat
    25681 tttctgataa ttcggtatat tcttaacaga ctatttaaga aataaggaga caaaaagatg
    25741 aagaaaaaat ttgccctatc gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt
    25801 ggagaagtga agtctggagc agtcaacact gctggtaact cagtagagga aaagacaatt
    25861 aaaatcgggt ttaactttga agaatcaggt tctttagctg catacggaac agctgaacaa
    25921 aaaggtgccc aattggctgt tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa
    25981 atcgaagtag tcgataaaga taataagtct gaaacagctg aggctgcttc agttacaact
    26041 aaccttgtaa cccaatctaa agtatcagca gtcgtaggac ctgcgacatc tggtgcgact
    26101 gcagctgcgg tagcgaacgc tacaaaagca ggtgttccat tgatctcacc aagtgcgact
    26161 caagatggat tgactaaagg tcaagattac ctctttattg gaactttcca agatagcttc
    26221 caaggaaaaa ttatctcaaa ctatgtttct gaaaaattaa atgctaagaa agttgttctt
    26281 tacactgaca atgccagtga ctatgctaaa gggattgcca aatctttccg cgagtcatac
    26341 aagggtgaaa tcgttgcaga tgaaactttc gtagcaggtg acacagactt ccaagcagcc
    26401 cttacaaaaa tgaaagggaa agactttgat gctatcgttg ttcctggtta ctataatgag
    26461 gctggtaaga ttgtaaacca agcgcgtggc atgggaattg acaaaccaat cgttggtggt
    26521 gatggattca acggtgagga gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc
    26581 tactttatct caggcttctc aactactgta gaagtttcag ctaaagctaa agccttcctt
    26641 gacgcttacc gtgctaagta caatgaagag ccttcaacat ttgcagcctt ggcttatgat
    26701 tcagttcacc ttgtagcaaa cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat
    26761 aaccttgcta aaacaaaaga ttttgaaggt gtaactggtc aaacaagctt cgatgcagac
    26821 cacaacacag tcaaaactgc ttacatgatg accatgaaca atggtaaagt tgaagcagca
    26881 gaagttgtaa aaccataata gaaaaatgtt gaaataggga atgagccttt gactcactcc
    26941 ctgtttcgat atttaatact cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga
    27001 ttatatatgt gactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa
    27061 cctgcggcta gtttcctagt ttgctctttg attttcattg agtataagaa cctatcaaaa
    27121 agtgagggaa acccctcgga attataaata gaaagagtga atcttatgct ccaacaactc
    27181 gtaaatggtt tgattctagg tagtgtttac gcgctgttag ccctaggata taccatggtt
    27241 tacggaatta tcaagctcat caacttcgcc catggtgata tttatatgat gggagccttt
    27301 atcggttatt tcttgatcaa ttctttccaa atgaatttct ttgtagcgct tattgtagct
    27361 atgctagcga cagctattct tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc
    27421 cactctactc gtattgctgt tttgattacg gctattgggg tttctttcct attggagtat
    27481 ggaatggtct atctggttgg tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt
    27541 cgatatgatt tgggaccaat tagcttaaca aatgtgcagt taatgatttt gggcatttcc
    27601 ttgattttga tgattttgtt acaagtcatt gtccaaaaga ctaagatggg gaaagccatg
    27661 cgtgcagtat cagtagatag cgacgcggcg caattgatgg ggatcaatgt aaaccgtacg
    27721 attagcttta ccttcgcttt gggttctgct cttgcgggtg cggctggtgt tctgattgct
    27781 ctttattata actctcttga gcctttgatg ggggttactc caggtcttaa atctttcgtt
    27841 gccgcagtac ttggtggtat cggaattatt cctggtgctg ctcttggtgg ctttgtgatt
    27901 ggtctattgg aaacctttgc gacagccttt gggatgtcag atttccgtga tgccattgtt
    27961 tatggaatct tgttgttgat cttgattgtc cgcccagctg gtatccttgg taagaatgtg
    28021 aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta atattctatg gttactcctt
    28081 ttgttagctg gctatagctt gattagtgta ctggtttcag tcggagtact taatctattc
    28141 tatgtacaga ttttacaaca aattggaatt aatattattt tggctgttgg tctcaactta
    28201 atcgttggtt tttcaggaca attttcactt ggtcatgctg gtttcatggc gattggtgcc
    28261 tatgcagcag ctattattgg ttctaaatca ccaacctacg gtgccttctt tggagctatg
    28321 cttgtagggg ctttgctttc aggagcagtt gccttacttg tcggcattcc aaccttgcgc
    28381 ttgaaggggg actatcttgc ggtagcaact ctaggtgttt ctgaaattat ccgtatcttt
    28441 atcatcaatg gtggaagcct tacaaatggt gcggcaggta tcttagggat tcctaacttt
    28501 acaacttggc aaatggttta cttctttgtc gtgattacaa ccattgcaac cttgaacttc
    28561 ttgcgtagcc caattggtcg ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag
    28621 tcagttgggg ttaatacgac taaaattaaa atcatcgctt ttgtctttgg tgccattact
    28681 gcaagtattg ctgggtcact tcaggcagga tttatcgggt ctgttgtacc gaaagattac
    28741 accttcatca actcaatcaa cgttttgatt attgttgtat ttggtggact cggttccatt
    28801 acaggtgcga ttgtttcggc tattgttctg ggaattttga atatgcttct ccaagatgtt
    28861 gctagtgtgc gtatgattat ttacgctttg gccttggtat tggtaatgat tttcagacca
    28921 ggtggactcc ttggaacgtg ggaactgagc ctatcacgtt tctttaaaaa atctaagaag
    28981 gaggaacaaa actaatggca ttacttgaag taaaacagtt aaccaaacat tttggtggtc
    29041 taacagctgt tggagatgtg actcttgaat tgaacgaagg ggaactggtt ggattaatcg
    29101 gtccaaacgg agctgggaaa accacccttt tcaacctttt gaccggtgtt tatgaaccaa
    29161 gcgagggaac agtaacccta gatggtcacc ttttgaatgg gaaatcacct tataagattg
    29221 cctctttggg acttggacgt actttccaaa atatccgtct ttttaaagat ttaacagttt
    29281 tagacaatgt tttgattgct tttggaaacc atcacaaaca gcatgttttt actagtttct
    29341 tacgcttacc agctttttac aagagtgaaa aagaattaaa ggctaaagct ttggaattgt
    29401 tgaaaatctt tgatttagat ggtgatgcag agactcttgc taaaaatctt tcctacggac
    29461 aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga acctaaaatt ctcttcttag
    29521 atgaaccagc agcaggtatg aacccacagg aaacagccga attgactgag ttaattcgtc
    29581 gtatcaaaga tgaatttaag attacaatca tgttgattga acacgatatg aatctggtca
    29641 tggaagtaac agaacgtatc tacgtacttg aatatggccg tttaattgct caaggaactc
    29701 cagacgaaat taagaccaat aaacgcgtta tcgaagctta tctaggaggt gaagcctaat
    29761 gtctatgtta aaagttgaaa atctttctgt gcattacggt atgatccaag cagtccgtga
    29821 tgtaagcttt gaagttaatg aaggagaagt tgtttccctt atcggtgcca acggtgcagg
    29881 taagacaact attcttcgca ccttgtcagg tttggttcga ccaagttcag gaaagattga
    29941 atttttaggt caagaaatcc aaaaaatgcc agctcagaaa attgtggcag gtggtctttc
    30001 acaagttcca gaaggacgcc acgtctttcc tggcttgact gttatggaaa atcttgaaat
    30061 gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga aggttttctc
    30121 acgctttcct cgtcttgaag aacgtaagaa ccaagatgca gctactcttt caggagggga
    30181 acaacaaatg cttgccatgg gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga
    30241 tgaaccatca atgggacttg ccccaatctt catccaagag atttttgata tcattcaaga
    30301 tattcagaag caaggaacaa ccgtcctctt gattgaacaa aatgccaata aagcacttgc
    30361 aatctctaac cgaggatatg tactggaaac agggaaaatc gtcctatcag gaacaggaaa
    30421 agaactcgcc tcatcagaag aagtcagaaa agcatatcta ggtggctaaa acaatccagt
    30481 ggattgtttt agtcggcaga tgaagattac gaagtaatca tcattatagt ctgggagact
    30541 agtttaggtt gcagataaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
    30601 ggcagatgga gattacgaag taatcatcaa tatagtccgg gggacctttt tagtcggtgg
    30661 atagagattg caaacaaata tgcatctaca ttgaaagctt aatttctaat aattgaaaaa
    30721 atcgaatgaa aaatttctta ccttcattca cagagctcta tttcagagct ctttttgcta
    30781 gcttattcat acttttttga atttcgaaaa agaaatgtaa gcgtttgata gatttacaaa
    30841 aagattgtat aatggggata agaatagaaa aggagaagtc tcatggcagt taaagatttt
    30901 atgacccgca aggtagttta tattagtcca gatacaacag tatctcatgc agcagatttg
    30961 atgagagagc aaggtttgca ccgtctgcct gttatcgaaa atgatcaatt agttggtttg
    31021 gtgactgagg gaaccattgc acaagcaagt ccatctaaag caacaagtct ttctatctat
    31081 gagatgaatt atcttctgaa taagacaaaa gtaaaagatg tcatgattcg cgatgttgtc
    31141 actgtctcag gctatgctag tctagaagat gcaacttatc tgatgttgaa aaataagatt
    31201 ggtattctcc ctgtcgtaga taactatcaa gtatacggag ttattactga ccgtgatgtt
    31261 ttccaagcct ttcttgaaat tgcaggttat ggcgaagaag ggattcgtgt acgctttgtt
    31321 acagaagatg aagttggtgt tcttggaaaa attgtttctt tgattgtaga agaaaatttg
    31381 aatatctccc atacagtcaa tattccgcgt aaggatggta aggtgattat cgaagtgcaa
    31441 atcgatgaat caattgattt accagccttg aaagaaaaat ttgaagcaaa tggtattcaa
    31501 gtggaagaaa tcgctcgcac ttcagcaaaa gtcttgtaag aagggaagtc caaaggcttc
    31561 ttttttcatg aaaaggggat tagagcaaaa gatggaaaga aatgataaaa tatgctataa
    31621 tgaaataatg taaaaaagga gtatttatgg acatttcagt aattcgtcag aaaattgacg
    31681 caaatcgtga aaaattagct tctttcaggg ggtctctttg acctcgaagg gctagaggaa
    31741 gagattgcca tcttggaaaa caagatgaca gaacctgatt tttggaacga taatattgcg
    31801 gcccaaaaaa cgtcgcaaga attgaatgaa ttaaaaaaca cttacaatac cttccataag
    31861 atggaagagt tgcaggatga agtcgaaatt ttattggatt ttttggctga agacgagtca
    31921 gtgcatgatg aactggtagc gcagttagcc gaacttgata agataatgac cagctacgag
    31981 atgactctac tcttgtcaga accttatgac cacaacaatg ccatcttgga aatccatcca
    32041 ggttctggtg gtactgaggc gcaggactgg ggtgatatgt tgcttcgtat gtatactcgt
    32101 tatggtaatg ctaaaggctt taaagtggaa gtgttggatt accaagcagg tgatgaggct
    32161 ggtattaagt cggtaacttt atcatttgaa gggcctaatg cctatggtct cctcaagtca
    32221 gaaatgggtg ttcaccgctt agtgcgaatc tcaccatttg actctgccaa acgtcgccat
    32281 acctctttca catctgtaga agtgatgcca gaattggatg atactattga agtggaaatc
    32341 cgtgaagatg atatcaagat ggataccttc cgttcaggtg gtgccggtgg acaaaacgtc
    32401 aataaggttt caacaggtgt acgtttaacc cacattccaa ctggaattgt tgtccaatca
    32461 acagtggatc gtacccagta tggaaataga gatcgtgcca tgaagatgtt gcaggctaag
    32521 ctctatcaaa tggagcaaga taagaaggct gcggaggtag attctctcaa aggtgagaaa
    32581 aaggagatca cttggggaag ccaaatccgt tcttatgtct tcacgcctta tactatggta
    32641 aaagatcacc gaactagctt tgaggttgct caggtagata aggttatgga tggggaccta
    32701 gatggtttta tcgatgctta tctcaagtgg cgaattagct aagatagaaa ggaactcaca
    32761 tgtcaattat tgaaatgaga gatgtcgtta aaaaatacga caacggaacg actgctctac
    32821 gcggtgtttc ggtcagcgtt caaccggggg aatttgctta catcgtagga ccttcaggag
    32881 cagggaagtc aacttttatt cgttctctgt atcgtgaagt aaaaatcgat aaaggaagtc
    32941 tatcagttgc tggttttaat ctggttaaga tcaaaaagaa agatgtcccg cttctacgtc
    33001 gtagtgttgg ggttgtcttc caagattata aattgttacc aaagaaaact gtctatgaaa
    33061 atattgctta cgctatggaa gtaatcgggg aaaatcgccg taatatcaaa agacgagtga
    33121 tggaagtttt ggacttggtt ggattgaagc ataaggttcg ttctttccca aatgaactct
    33181 caggtgggga gcaacagcga attgcgattg cgcgtgcaat tgtaaataat cccaaagtat
    33241 tgatagctga tgagccaaca ggaaatctgg atccggataa ttcatgggaa attatgaatc
    33301 tcttggaacg gattaaccta caagggacaa ctattttgat ggcgactcat aatagccaga
    33361 ttgtaaatac cttgcgccac cgtgtcattg ccattgaaaa tggccgtgtc gttcgtgacg
    33421 aatcaaaagg agagtatgga tacgatgatt agtagatttt ttcgccattt atttgaagcc
    33481 ttaaaaagtt tgaaacgaaa tggttggatg acagtagctg ctgtcagttc agtcatgatt
    33541 actttgacct tggtggcaat atttgcatct gttattttca atacagcgaa actagctaca
    33601 gatattgaaa ataatgtccg tgtagtagtt tatatccgaa aggatgtgga agataatagt
    33661 cagacaattg aaaaagaagg tcaaactgtt acaaataatg actaccacaa ggtatatgat
    33721 tctttgaaga acatgtctac ggttaaaagt gttacctttt caagtaaaga agaacaatat
    33781 gaaaaattaa ccgagataat gggagataac tggaaaatct ttgaaggaga tgccaatcct
    33841 ctctatgatg cctatattgt agaggcaaac actccaaatg atgtaaaaac tatagccgaa
    33901 gatgctaaaa aaattgaagg tgtctctgag gttcaagatg gcggtgccaa tacagaaaga
    33961 cttttcaagt tagcttcatt tatccgtgtt tggggactag ggattgctgc tttgttaatt
    34021 tttatcgcag ttttcttgat ttcaaatacc attcgtatta ccattatttc ccgcagtcgc
    34081 gaaattcaaa tcatgcgctt ggtcggagct aaaaacagtt atatccgtgg accgttcttg
    34141 ttagaaggag cctttatcgg tttattggga gctatcgcac catctgtttt ggtctttatt
    34201 gtttatcaaa ttgtttacca atctgtcaac aaatcgttgg tagggcaaaa tctatccatg
    34261 attagtccag atttatttag tccgttgatg attgccctac tatttgtgat tggggttttc
    34321 attggttcat tgggatcagg aatatccatg cgccgattct tgaggattta ggtaaaatag
    34381 ctgcttttat gaggagattg taaaatctcc ttttttgcta caagagtttt tgaaaagaga
    34441 tgcgtagaag aaaagagctt ccaaagaagt cccccagaga agacttcttg atggctaatt
    34501 tgaacttgaa atttggcgca ctagaaattt tttagaaaaa agtgtaattt ttttataaaa
    34561 gcctttacaa aaaaaattaa agtggtataa ttaaatcata gaaggtgcaa acgttttcgt
    34621 aaaacgtttg cctaaataaa aataaaggag atttgaatga tgaaagatac attcaaaaat
    34681 gtcttgtctt tcgaattttg gcaaaaattc ggtaaggctt tgatggtagt tatcgcggtt
    34741 atgccggctg ctggtttgat gatttcaatc ggtaagtcta tcgtgatgat taacccaacc
    34801 tttacaccac ttgtcatcac aggtggaatt cttgagcaaa tcggttgggg ggttatcggt
    34861 aaccttcata tcttgttcgc cctcgctatt ggaggaagct gggctaaaga acgcgctggt
    34921 ggtgctttcg ccgctggtct tgccttcatc ttgattaacc gtatcactgg tacaatcttt
    34981 ggtgtatcag gcgatatgtt gaaaaatcca gatgctatgg taactactct cttcggtgga
    35041 tcaatcaaag ttgctgatta cttcattagt gttatggaag ctccagcctt gaacatgggt
    35101 gtgttcgtag ggattatttc tggttttatt ggagcaactg cttacaataa atactataac
    35161 ttccgtaagc ttcctgatgc actttcattc tttaacggaa aacgttttgt accgtttgta
    35221 gttattcttc gttcaataat tgctgcaatt gtacttgctg ctttctggcc agtagttcaa
    35281 acaggtatca atagctttgg tatctggatt gccaactcac aagaaactgc tccaatcctt
    35341 gcaccattct tgtatggtac attggaacgt ttgctcttgc catttggtct tcaccacatg
    35401 ttgactatcc caatgaacta cacagctctt ggtggtactt atgaggtctt aactggtgca
    35461 gcaaaaggta gccaagtatt tggtcaagat ccactttggc ttgcatgggt aacagacctt
    35521 gtaaacctta aaggtactga tgctagtcaa taccaacact tgttagatac tgttcaccca
    35581 gctcgtttca aagttggaca aatgattggt tcattcggta tcttgatggg tgtggttgtg
    35641 gctatctacc gtaatgttga tgctgacaag aaacataaat acaaaggtat gatgattgcg
    35701 acagctcttg caacattctt gacaggggtt actgaaccaa tcgaatacat gttcatgttc
    35761 gtcgcaacac ctatgtatct tgtttactca cttgttcaag gtgctgcctt cgctatggct
    35821 gacgtcgtaa acctacgtat gcactcattc ggttcaatcg agttcttgac tcgtacacct
    35881 attgcaatca gtgctggtat cggtatggat atcgttaact tcgtttgggt aactgttctc
    35941 tttgctgtaa tcatgtactt tattgcaaac ttcatgattc aaaaattcaa ctacgcaact
    36001 ccagggcgta acggaaacta cgaaactgct gaaggttcag aagaatctag caacgaagtg
    36061 aaagttgcag caggctctca agctgtaaac attattaacc ttcttggtgg acgtgcaaac
    36121 atcgttgatg ttgatgcatg tatgactcgt cttcgtgtaa ctgttaaaga tgcagataaa
    36181 gtaggaaatg cagagcaatg gaaagcagaa ggagctatgg gtcttgtcat gaaaggacaa
    36241 ggggttcaag ctatctacgg accaaaagct gacgtattga aatctgatat ccaagatatc
    36301 cttgattcag gtgaaattat tcctgaaact cttccaagcc aaatgactga agcacaacaa
    36361 aacactgttc acttcaaagg tcttactgag gaagtttatt cagtagcaga cggtcaagtt
    36421 gttgctttgg aacaagtaaa agatccagta tttgctcaaa aaatgatggg tgatggattt
    36481 gctgtagaac ctgcaaatgg aaacattgta tctccagttt caggtactgt atcaagcatc
    36541 ttcccaacaa aacatgctct tggtattgtg actgaagcag gtcttgaagt attggttcac
    36601 attggtttgg acacagtaag tcttgaaggt aaaccattta cagttcatgt tgctgaagga
    36661 caaaaagttg tagcaggtga tctccttgtc acagctgact tggatgctat ccgtgcagca
    36721 ggacgtgaaa cttcaacagt agttgtcttc acaaatggtg atgcaattaa atcagttaaa
    36781 ttagaaaaaa caggttctct tgcagctaaa acagcagttg ctaaagtaga attgtaatat
    36841 acttgaggtt ggaagctgta ttccaacctc ttattttggg agaaaagaat gaaattttta
    36901 acactcaata ctcacagttg gatggagaaa gaagcagagg aaaaattcca gattttgctt
    36961 gaggatattc ttgaaaagga ctatgatttg atttgtttcc aagaaatcaa tcaggagatc
    37021 acttcgccag aggtggaggt taatgacctt tatcaagctt tgccagcagc tgagcttatt
    37081 caccaagatc actatgttag actcttggtt gaaaagttgt tagagcaagg gaaaaattac
    37141 tactggacct gggcttataa ccatatcggc tatgaccgct accacgaagg tgtggctatc
    37201 ttgtctaaaa cacctattga agccagagaa attttggttt cagatgtgga tgatccaaca
    37261 gactatcata ctcgccgtgt tgccctagct gaaactgtag tcgatggcaa ggagctagca
    37321 gttgccagtg ttcatctctc ttggtgggat aaaggtttcc aagaagaatg ggcacgattt
    37381 gaggctgtct tgaaaaaatt gaacaagcca cttttactag ctggagattt taacaaccca
    37441 gcaggtcagg aaggttacca agctatttta gctagtccat taggcttaca agacgcattt
    37501 gaagttgctc aagagaaaag tggtagctat actgttccgc ctgaaattga tggctggaaa
    37561 gggaacactg aaccccttcg aatcgattat gtctttacta ccaaagagtt agcggtggaa
    37621 aatttacatg tcgtatttga tggtaacaag agtccacaag tgagtgatca ctatggcttg
    37681 aatgctatgt taaactggaa ataataactg aaaagaggtt ggaactataa aattccagcc
    37741 ttttcttact agagaagcta ctggaaatag cctaaataag tgagactact gtaatggaat
    37801 aaaatatggt ataattgata agatagatag aatcgaggat gttatgtcat ttacgaaatt
    37861 tcaatttaaa aactatatta gagaagcctt gaaggagtta aaatttacaa ctccaacaga
    37921 ggtgcaagac aagttgattc ctattgtttt ggcaggtcgt gacctagtag gagaatcaaa
    37981 aacaggttca ggtaagactc atactttctt gttaccgatt tttcagcaat tagatgaagc
    38041 tagcgatagt gtacaagcag tgattactgc accgagtcgt gagttggcta ctcaaattta
    38101 ccaagtagcg cgtcagattt cagctcactc agatgtcgaa gttcgtgtgg ttaattatgt
    38161 gggtggtacg gataaggctc gccagattga gaaattggca agcaatcagc ctcatattgt
    38221 tattggaaca ccaggccgta tctacgactt ggttaaatct ggtgatttag ctattcataa
    38281 agccaagaca tttgttgttg atgaagcgga tatgaccttg gatatgggat tcttggaaac
    38341 tgttgataag attgctggca gtcttccaaa agacttgcaa ttcatggtct tctcagcgac
    38401 tatcccacaa aaactgcaac cattcttgaa aaaatactta tccaatcctg ttatggagaa
    38461 aattaagacc aaaacggtta tttctgacac cattgataat tggttgattt cgaccaaggg
    38521 acatgataag aatgctcaaa tttaccagtt gactcagttg atgcagccgt atttggcaat
    38581 gatttttgtt aacactaaaa cgcgtgctga tgaattgcat tcatatctga ctgctcaagg
    38641 cttgaaggtt gcaaaaatcc atggcgatat tgcccctcgt gaacgcaagc gaatcatgaa
    38701 tcaggtgcaa aatctggatt ttgagtatat tgtcgcaaca gatttagcag cgcgtgggat
    38761 tgacattgaa ggtgtcagcc atgtcatcaa tgatgccatt ccgcaagact tatctttttt
    38821 tgttcaccgt gttggtcgta ctggacgaaa tggcctacca ggtacagcta ttacccttta
    38881 tcagccaagt gatgactcgg atatccgtga gttggagaaa ttgggaatca agtttagtcc
    38941 taagatagtc aaagatgggg aatttcaaga tacctatgac cgtgatcgtc gtgccaaccg
    39001 tgagaaaaaa caagataaac ttgatatcga aatgattggt ttggttaaaa agaaaaagaa
    39061 aaaagtcaaa ccgggttata agaagaaaat tcaatgggcg gttgatgaaa agcgccgtaa
    39121 aaccaagcgt gctgaaaatc gcgctcgcgg tcgtgcagag cgtaaagcta aacgccaaac
    39181 attttaatag aaattgttgg agtactgagc tccaactttt ttatttatga gaacgaacta
    39241 tctaaaccga aacactacat taaagactgc aaattgcgat taaaaatggt ataatgataa
    39301 agttatatag tcccgataag atggtaggta tttattacga agagttttcc tatcagtagt
    39361 ttgcaattcg caaagaatga cggcttcgag gtcgctcgtc tttctacggt tgctgccata
    39421 ctgcggtcac cctatgcgcc ttactagctt cagaaataaa aaagtatcgt ttttatttct
    39481 gaagctagta actcttagat aatggaaatt cctatcagta ctttgtaact ctataacaat
    39541 attttttaag gggggacatt tttatgtcag aacgtaaatt attcacgtct gaatctgtat
    39601 ctgaggggca tccggataag attgcagacc aaatttcaga tgcgattttg gatgctattt
    39661 tagcaaagga tccagaggcg cacgttgctg ctgaaacagc tgtatatact ggttctgtcc
    39721 acgtttttgg tgaaatttct acaaatgcct atgtggatat taaccgtgtg gttcgtgata
    39781 ccattgcaga gattggttat accaatacag aatatggatt ttctgctgag acggtgggag
    39841 tacacccatc tttggtggaa caatctcctg acatcgctca aggtgttaac gaagccttgg
    39901 aggttcgtgg aaatgctgat caagatccac tggacttgat tggagcaggt gaccaagggc
    39961 tcatgtttgg atttgcagta gatgaaacag aagagctcat gccattgcca attgcactca
    40021 gtcataaatt ggttcgtcgt ctggcagaac ttcgtaagtc tggagaaatt agctatctcc
    40081 gtccagatgc aaaatcacaa gttacagttg agtacgatga aaatgaccgt ccggtacgtg
    40141 tagatacagt cgttatttct actcagcatg atccagaggc cactaatgaa caaatccatc
    40201 aagatgtgat tgacaaggtc atcaaagaag ttattccatc ttcttatctt gatgataaga
    40261 caaaattctt tatcaatccg acaggtcgtt ttgtaatcgg tggtcctcaa ggggactcag
    40321 gtttgactgg tcgtaagatt attgtagata cttatggtgg ctactctcgt catggtggtg
    40381 gtgccttctc tggtaaagat gcgactaagg tggatcgttc agcctcttat gcggctcgct
    40441 atattgccaa gaatatcgtt gcagcagacc ttgctaagaa ggcagaagtg cagttggcct
    40501 atgctatcgg tgttgcgcaa cctgtttctg ttcgtatcga tactttcggt acaggaacag
    40561 tagctgaaag tcaacttgaa aaagcggctc gtcaaatctt tgaccttcgc cctgcaggga
    40621 ttatccaaat gctggacctt aagcgtccaa tttaccgtca aacatcggct tacggtcaca
    40681 tgggacgtac agatattgat cttccatggg aacgtttgga taaggtagat gctttgaaag
    40741 aagcagtaaa ataagatttt aagaggggaa cgtcctctct tttttatagt ttttaactgt
    40801 actgggatac tgttctgaaa atccattttg agaaagtaga gatttacatg tatagtagat
    40861 tgaaactaga atagtacacc tcaacttcta aaacattgtt agaaatcgat ttgactgtcc
    40921 tgatcgattt ctcctgttct tgtttcattt tactatattt ctttaaaaat gataaaggtt
    40981 gagatttctc ctcgtaatag ataatcatgg ggatatttca agccaaagtt ttattcgtta
    41041 tcacttgact attgcaaggt tttctagagc aacagagtca tggaatggac tcatggttga
    41101 gatttctcct tgttgcttgg acttcattca aaagtctgtt acccaagcct tgttcaaact
    41161 tccaatacac tagctgtttc catagcatga cttctgtact agactttctt ttccgaataa
    41221 atagatagaa ccacagaatc tagtaaacct agaattaaaa ttatggtata atattagcaa
    41281 taaaagaaat ctggaggatt agaatcatgg tatcaacgaa aacacaaatt gctggttttg
    41341 agtttgacaa ttgcttgatg aatgcagcag gtgtggcttg tatgacgata gaggagttag
    41401 aagaggtcaa aaactcagcg gcaggaacct ttgttactaa gacagcgacc ttggacttcc
    41461 gtcaggggaa tcctgagcca cgctaccaag atgttccact tggttccatc aactctatgg
    41521 gcttgccaaa taatggctta gactattatt tggattatct tttagatttg caggaaaaag
    41581 agtcgaaccg aactttcttc ttatctctgg tcggcatgtc tccagaggaa acccatacta
    41641 ttttgaaaaa agtccaagag agtgattttc gtggtctgac tgagctaaat ctttcctgtc
    41701 caaatgttcc aggtaaacct cagattgcct atgattttga gacaacagac cggattttgg
    41761 cagaagtgtt tgcttacttc accaaacctc ttggaattaa attgccacct tattttgata
    41821 ttgttcactt tgatcaagcg gcagctattt tcaacaaata tccgctcaag tttgtcaact
    41881 gcgttaactc tatcggaaac ggcctctata tagaagatga atctgtcgtt attcggccta
    41941 agaatggttt tggtggaatt ggtggagaat acatcaaacc gactgctcta gccaatgttc
    42001 acgcctttta tcaacgttta aatcctcaaa tccaaattat cggaacaggt ggcgttctga
    42061 ctggtcgaga tgcctttgaa cacatcctct gtggagcaag tatggtgcag gtgggaacga
    42121 cccttcacaa agaaggcgtc agtgcttttg accgcattac caatgaactg aaagcaatca
    42181 tggtggaaaa aggctacgag agcttagaag atttccgtgg gaaattgcgc tatattgact
    42241 aaattaaatc gaaaaatctg aagaaaggag agacgatgct agccattgaa gaaagtcaga
    42301 agttgacttt atcaaattta ccgagcctga gcctatttac agggacagat cagggtcagt
    42361 ttgaagtgat gaagagtcaa gtgttgaaac agattgggta tgattctgct gacctcaact
    42421 ttgcctactt tgatatgaaa gaagtagttt acaaggatgt ggaactggag ttggtcagcc
    42481 ttcctttctt tgcggatgag aaaatcgtga tattagatta ttttatggat atcacgactg
    42541 ctaagaaacg ctttttgaca gatgatgagc ttaagtcatt tgaggaatac cttgacaatc
    42601 cttctccaac aaccaagttg ataatctttg cagaaggaaa gctggatagc aaaagacggt
    42661 tagtcaaatt acttaagcgt gatgccaagg tcttcgatgc agtagaagta aaagaacaag
    42721 aattgcgcca gtatttccaa aagtggagtc agaaacaagg tctgcagttt accaatcatt
    42781 cttttgaaaa tctcctcatc aagtcggggt ttcaatttag cgaaatccag aaaaatcttc
    42841 tctttttaca gtcctataag gcgaattctg ttatcgagga agaggatatt gttaacgcaa
    42901 ttcccaagac cttgcaggac aatatttttg atttaactca gtttattctg actaaaaaga
    42961 tggatcaggc gcgcgatttg gtgagagact tgaccttgca aggggaagat gaaatcaaac
    43021 tgattgcagt catgctggga caatttcgga cttttactca ggtgaagatt ttggcggagt
    43081 ctggccaaac agaatcgcag attgcaagta gtttaggtag ttatctggga cgtaacccaa
    43141 atccttatca aatcaagttt gcattaagag attcgagagg actttctttg agctttctga
    43201 agcaagctat ttcctatttg attgagacag actatcagat taagacaggt ctttatgaaa
    43261 aaggtttcct ttttgaaaag gcactcttac agattgctag tcaggtcaat tgacatttgt
    43321 tgaaactaca acccacgtca ggattatctt gtcgtgggtt tcttgctgat ttgcttattt
    43381 tattagaaag tatgtcagtg atttccttat ctataagcaa aaaacttgaa aaaagtgaat
    43441 gtttgcgtta tcattttcat atagaaagaa aaagaggtat tacagatggc tattatctta
    43501 ccagaacttc catatgcata cgacgctttg gaaccataca tcgatgcgga aacaatgcat
    43561 ttgcaccatg acaaacacca tcaaacttat gtcaacaatg ccaatgcagc tttagaaaaa
    43621 caccctgaaa tcggtgaaga ccttgaagcc ttgcttgctg atgtagaatc tatcccagct
    43681 gatatccgtc aagcacttat caacaatggt ggcggacact tgaaccacgc tcttttctgg
    43741 gaattgatga ctcccgagaa aacagctcct tcagcagaac tagcagcagc aatcgatgca
    43801 acatttggtt catttgaaga atttcaagca gccttcactg cagcagcaac aactcgtttt
    43861 ggttcaggtt gggcatggtt ggttgtcaac aaagaaggga aacttgaagt gacttcaaca
    43921 gcaaaccaag acacaccaat ctcagaaggt aaaaaaccaa tcttgggctt ggacgtttgg
    43981 gaacatgctt actacgtgaa ataccgcaac gtgcgtcctg actacatcaa agctttcttt
    44041 tcagtaatca actggaataa agtagatgaa ttgtacgcag ctgctaaata ataatagttg
    44101 gagggaagaa ttgttcttct cttttttagg ttataagatt ctggtatgac aaaatcgtca
    44161 gacttttttc atttttatga gaaacgtgct aactgctaga aattatggta gaataaagta
    44221 ttaagtaatc gtggaaaaag gatatctatg cgaaaagaaa ttgcacctga attatacaac
    44281 tataacaagt ttcctggtcc ggagttccat ttacacgggg acaaggtcga aacggaaggg
    44341 atagcttttt ccttggttga aaatatcaag gatgcctttg atgtgacgac ttttaatcag
    44401 cgtttttcag aagtcttaac caagtttgat tatatcgtgg gggactggag caacgaacag
    44461 cttcgcctac gaggttttta caaggatgac cgaacagaag aaaaacttga aaaaatcagt
    44521 cgtttacaag actacctttt agagtattgt agttatggtt gtgcctattt tgtcttagaa
    44581 aatgaagccc ctaagcgagc atcatttgac aagaaaatgc gtaagacgga agaagaaaca
    44641 ccttctagaa aaggaaagaa accgactcaa actaaacgaa agtcgaatgc agataagaaa
    44701 aatagacgtc gtcagaaaga ccagcattct cagaaagagg acaagggaca acgtcatttt
    44761 gtcattcgtc agaagtgagt agagaataag gagaagaaat gaaaccctca attcatagtt
    44821 tggcacatca aaccatgcag aaatgggtat tggagcaggg agaaaagaaa ttccgtgcag
    44881 atcaaatctg ggaatggctc taccgtaaac gtgtgcagtc atttgaagaa atgaccaacc
    44941 tttccaagga tttgattgct aagctcaatg accagtttgt ggtcaatccc ttgaaacaac
    45001 gtattgtgca agagtctgct gatggtacag tcaaatatct ctttgaattg cctgacggta
    45061 tgttaattga gactgtactc atgcgtcaac actatggttt atctgtctgt gtgaccactc
    45121 aggtcggctg taatatcggt tgtaccttct gtgcatctgg tttgattaag aagcagcgtg
    45181 acctcaataa cggtgaaatc gtagcgcaaa tcatgctggt tcagaaatac tttgctgatg
    45241 aaggggttca ggtcaatctt gccgtgtctc ttcacgcacc caacaatgaa cttcgttcaa
    45301 gtatcatgaa gattaaccgt gcctttccga ttgaaaaact ctttgctgct attgaatact
    45361 acattgagac aacaaaccgt cgtgtaacct ttgaatatat catgcttaat gaagtcaacg
    45421 acggtgtaga acaagccttg gaattggctg aattgctcaa gaacatcaag aaattgtctt
    45481 atgtaaactt gattccttat aacccagtta gtgagcatga ccaatatagc cgtagtccca
    45541 aagagcgcgt gttggctttc tatgatacgc ttaagaaaaa agggggcaac tgtgtggttc
    45601 gtcaagagca tggtacagat attgacgcag cttgtggaca attgcgcttt aacacaatga
    45661 aacgtgaccg ccagaaggca gtcgcagcgg taaatcctta atgatgaaga agacttataa
    45721 tcatattttg gtctggggag tcattttcta tagcatttgc attgtctgtt tttgctttac
    45781 tcctcaagaa caatctaccg tgggagtggg aactccaggt attcagcatc ttggacgcct
    45841 ggtttttctt ttgactcctt tcaattctct ctggaaactg ggcgaagtga gtgacattgg
    45901 acaattatgt tggatttttt tacaaaatat cctcaatgtc ttcttgtttt ttcctctgat
    45961 tttccaactc ctttatctat ttccaaattt gcggaaaaca aaaaaggtct ttctttttag
    46021 ttttcttgtg agtcttggaa tcgagtgtac gcaattaatc ttggactttt tctttgattt
    46081 caatcgcgtc tttgagattg atgatttgtg gaccaacact ttgggtggct atctggcttg
    46141 gctcctttat aaacgattac ataaaaacaa ggtaaggaat taaaatgagt attttagaag
    46201 ttaaaaatct gagtcacggt tttggtgacc gtgcaatttt tgaagatgtg tccttccgtc
    46261 tcctcaaggg agaacatatc ggcctggtcg gtgccaatgg tgaaggaaaa tcaaccttta
    46321 tgagtatcgt gactggtaaa atgctgccag atgaaggaaa ggttgagtgg tccaaatatg
    46381 tgacggctgg ttacttggat cagcactctg tccttgctga aagacagtcg gtgcgtgatg
    46441 ttctccgtac ggcttttgat gagcttttca aagctgaagc tcgtatcaat gacctctata
    46501 tgaaaatggc tgaagacggc gcggatgttg atgctctcat ggaagaagta ggagaacttc
    46561 aagaccgtct ggagagtcgt gatttctata ccttggatgc taagattgac gaagtagcgc
    46621 gtgctcttgg tgttatggac tttggcatgg atacggatgt aacttctttg tcaggtgggc
    46681 aaagaaccaa ggtgcttttg gcaaaacttc tccttgaaaa gcctgatatc ttgctgttgg
    46741 acgagccgac caactacttg gatgctgagc atattgattg gctcaagcgc tatctccaaa
    46801 actatgagaa tgcctttgtt ctcatttcgc acgatattcc attcctcaat gacgttatta
    46861 atattgtcta tcatgtggaa aatcaacagc tgacgcgtta ctctggtgac tactaccagt
    46921 tccaagaagt ttatgctatg aagaaatctc agctagaggc agcctacgaa cgccagcaga
    46981 aagagattgc agacctcaag gactttgtgg ctcgtaataa agcccgtgtt gcaactcgta
    47041 atatggctat gtctcgtcaa aagaaattgg ataagatgga tattatcgaa ctccaaagtg
    47101 agaaaccaaa accatccttt gatttcaaac cagctcgtac accagggcgc tttatcttcc
    47161 aagccaagaa cttgcaaatt ggttacgacc gtcctcttac taagccttta aatcttacct
    47221 tcgaacgcaa tcaaaaggtt gcgattattg gtgctaatgg tattggaaaa acaactctct
    47281 tgaagagtct cttgggcatt atctcgccaa tcgctgggga agtggagcgt ggagattatt
    47341 tagaacttgg ttattttgag caggaagtag aaggcggtaa tcgccaaact cctcttgaag
    47401 ctgtctggaa tgcctttcct gcccttaatc aagcagaagt ccgtgcagcc cttgcccgtt
    47461 gtggtttgac aaccaaacat attgaaagcc agattcaagt attatcaggg ggagagcaag
    47521 ccaaggttcg tttctgtctc ttgatgaatc gtgaaaacaa cgttttagtg ctggacgagc
    47581 cgaccaacca tttggatgtg gatgcaaagg atgagctcaa acgcgctctc aaagaatata
    47641 ggggatctat ccttatggtc tgccacgagc cagactttta tgaaggctgg atagaccaaa
    47701 tatgggattt taataattta acttaagaat gagagtaaaa gaaatacagt accgaatgtg
    47761 gatactgtat ttttttggtt tttaagattt aaaatcgtag tctttcacca cttcgatgct
    47821 gtcgattgtg atagcagtag ttggcttgtc tttttcatct ttttcggcct tagcaatctt
    47881 atccacaaca tccataccgt caatcacttg accaaagact gggtgtttgc catctagact
    47941 agggtttcca ccttctttgt aggcttcgat aattttctgt ggatacttgc ttgtagggag
    48001 tttagaagag gtatctgtag agctttggtt gatgaagaac tggctgccat tggtgtttgg
    48061 ttgaccagta ttagccatag caagagcacc acggatgtta tacaaataag gagtaatctc
    48121 gttcttgaaa ccagttcctt tgtctttagt cttatccttg tcatgccaga tggactgacc
    48181 acctgtaccg tccccttttg gatctccagt ttggaccata aagccatcga tgacacggtg
    48241 gaaggtaata ccgttatagt agccttcttt ggcatgagtg aggaaatttt caaccgctag
    48301 aggagcgagt ttagggaaga gtttaatgcg aatatcacct tggcttgtgt ggagaatcac
    48361 ttcggcttca tcttcagcaa cttccttaga tagttgtggg aaattggcgt tttcgtttgt
    48421 taatgtatcg tttaactcct tggcagattg ggcagctact ttggaacttt cttcagcagc
    48481 aagactggaa tcaacataat catcaccacg cagactgcgt tggacgctgc tacacccagc
    48541 tagggctaca gtagacagta aaagaagggt tgctagtttt ttcattctga tcctctcaaa
    48601 aattcattta taaccattgt accctaaaag gaagtggatt tcaaatatta gtagtttaca
    48661 gaactggctt agataatagt tgggatttca tcatcatctt gaaaatcttc aacttcttcc
    48721 ccagtaaatg gtttctgaaa agaacttagt ataaaacggg caattggttc aacgattagg
    48781 aaatttagag gaagagctgc aataaagttt acgaaccagg cgcttagata ggtattgggt
    48841 gtgatagcaa tgccattgta aaaaagtcca tagaaggaca tgaaagtgac cagctaattc
    48901 ttcttctgaa aaatgctcat gaagggcttg atccaggtcg caagatttca ttttagaaag
    48961 tccagtaact cccttcttct tcaggatatt ctttttcatg gtattattta gatggtcaag
    49021 tgagtcaaaa gaagtttcga caagagcata ccctttttct accagagttt ttagatgttt
    49081 aggggcatca ataccataag tgtactcaaa gtattttgga aaccagatct taagaaagct
    49141 cgtaaagcta gccagttctc taaacgctaa gaactagcta caacacagca tttacaacac
    49201 ttcatggttc acaccatggg gtgtttttta atgattttaa gcaaaattca cacttaattg
    49261 aaagttattt aaattagatg aaaatttatt ttttgaaaaa gtcggaaaac attgattcta
    49321 agcgaatttt gaatcatatt cgaatgggag tttttaaaat catgtttcag tggtaaaagt
    49381 accaaactgg attctccata gtaaaatgat atctcctgct agtgaatcat ctactaataa
    49441 gtccatattt cgcttgggaa cgggcttggg acttagaagc caagtatcca actctcgttc
    49501 aggcgatatg aagggtttga cttggtgttt tttataaaat ttttctaaaa tagtattcac
    49561 ggatagcatc tttcaaacat ttatttccta ttctaacaaa tttctaaata ctttaagaaa
    49621 tttatagtct gtcaagtttt tttcttgaca catgccagaa atctgctata atagaacatg
    49681 tgctaaatag ctcagctatt tcaccgaaat aaaaaattaa agaaatgaga ctttaaaaat
    49741 ggcagttaaa atccgtttga ctcgtatggg ttctaagaaa aaacctttct accgtatcaa
    49801 cgtagcagat tcacgttcac cacgtgacgg acgtttcatc gaaacagttg gaacttacaa
    49861 cccacttgtt gctgaaaacc aagtaacttt gaaagaagat cgcgttcttg catggttggc
    49921 taatggagct caaccttcag acacagtacg taacatcctt tcaaaagaag gcgtattgaa
    49981 aaaattccac gattctaaat tttcaaaata agtttaaagt aggttgacag atggatacga
    50041 ttgaaaatct cattattgcg attgtgaaac ccttaatttc acaaccagat gccttaacta
    50101 tcaagattga ggatacacca gaatttttgg aatatcattt gaatcttgat caaagcgatg
    50161 tgggtcgtgt aatcggtcgt aagggtcgca ctatttctgc gataagaacg attgtctact
    50221 ctgtcccaac tgaatacaaa aaagtaagaa tcgttattga cgaaaaataa gaagggcggg
    50281 acggatgtct cgcttttttg tgtggaatag aagatatagt aaaatgaaac aagaacagga
    50341 caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtaa aggtgtacta
    50401 ttctagtttc aatatactat aaaagattga acgtttaggc aattccaaga ctacttaatc
    50461 gaacattaa
//
LOCUS       DAVHIU010000002        47214 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVHIU010000002 DAVHIU010000000
VERSION     DAVHIU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.002"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            177..407
                     /locus_tag="VVN06_000055"
     CDS             177..407
                     /locus_tag="VVN06_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5561676.1"
                     /translation="MAQWTLRACRVNAGFTLRQVAKKVNKNFQTISKYEKDSTLIPFE
                     LLKELSELYQVKLDDIFLGDSTKKIELNQKEI"
     gene            423..1049
                     /locus_tag="VVN06_000056"
     CDS             423..1049
                     /locus_tag="VVN06_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU5561677.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIETWEAKPKHAHSTMNNLLLKAVTDMNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            1421..1873
                     /locus_tag="VVN06_000057"
     CDS             1421..1873
                     /locus_tag="VVN06_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561678.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            1870..2070
                     /locus_tag="VVN06_000058"
     CDS             1870..2070
                     /locus_tag="VVN06_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561679.1"
                     /translation="MTLNLDNMTQAEFDEVMAEIKEKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKNYQARA"
     gene            2072..2287
                     /locus_tag="VVN06_000059"
     CDS             2072..2287
                     /locus_tag="VVN06_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561680.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSAQFERENQQRI
                     ETPSEDLSPDYGRYIQLAGVRVYGGTE"
     gene            2284..2625
                     /locus_tag="VVN06_000060"
     CDS             2284..2625
                     /locus_tag="VVN06_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5561681.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRHSVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTVLLNADLESY
                     KETLKEVGGYV"
     gene            2618..2899
                     /locus_tag="VVN06_000061"
     CDS             2618..2899
                     /locus_tag="VVN06_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5561682.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEARDTVKPRLTGLISAQEVM
                     DELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYKISDIWKFLGVENGKV"
     gene            3034..3900
                     /locus_tag="VVN06_000062"
     CDS             3034..3900
                     /locus_tag="VVN06_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU5561683.1"
                     /translation="MTIYEAKGFQSNLVYPFDKIEPFQYIERFKPLVVPEGADIEEYK
                     RTQAPYCISGKVTPEKHGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKAISSALFGY
                     SYILYPTIKHSLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPASYQKIVEHGLDYPVPQTTAEPVKQNAASLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            3953..5422
                     /locus_tag="VVN06_000063"
     CDS             3953..5422
                     /locus_tag="VVN06_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU5561684.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNENTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAIDPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVSPEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            5775..5942
                     /locus_tag="VVN06_000064"
     CDS             5775..5942
                     /locus_tag="VVN06_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561685.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEIDDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            6026..6556
                     /locus_tag="VVN06_000065"
     CDS             6026..6556
                     /locus_tag="VVN06_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561686.1"
                     /translation="MTLKTFSSKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPV
                     ISLTYKDKGTLVAEYVEDHKLNKTFKRICDSFKDYHKQPGEAEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFATLEMLGDESISLLQKLD
                     VEGEYKGLAGYTEHLK"
     gene            6627..7136
                     /locus_tag="VVN06_000066"
     CDS             6627..7136
                     /locus_tag="VVN06_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561687.1"
                     /translation="MKQLAIEAITKPMKLRGISKGIAELDGQRLEIDLDSLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKHTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKQKRYYKHYQKFINYTRKGDSF
                     WLNGLSSLR"
     gene            8332..8751
                     /locus_tag="VVN06_000067"
     CDS             8332..8751
                     /locus_tag="VVN06_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU5561688.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELETLEGGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMKISELIDKLANPLERSVLRLFYL
                     NDLVVSEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
     gene            9240..12071
                     /gene="uvrA"
                     /locus_tag="VVN06_000068"
     CDS             9240..12071
                     /gene="uvrA"
                     /locus_tag="VVN06_000068"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5561689.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            12064..13125
                     /locus_tag="VVN06_000069"
     CDS             12064..13125
                     /locus_tag="VVN06_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5561690.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            13257..13655
                     /gene="spx"
                     /locus_tag="VVN06_000070"
     CDS             13257..13655
                     /gene="spx"
                     /locus_tag="VVN06_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU5561691.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            13721..14290
                     /locus_tag="VVN06_000071"
     CDS             13721..14290
                     /locus_tag="VVN06_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5561692.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            14377..14643
                     /locus_tag="VVN06_000072"
     CDS             14377..14643
                     /locus_tag="VVN06_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU5561693.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            14647..15066
                     /gene="ruvX"
                     /locus_tag="VVN06_000073"
     CDS             14647..15066
                     /gene="ruvX"
                     /locus_tag="VVN06_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5561694.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            15082..15387
                     /locus_tag="VVN06_000074"
     CDS             15082..15387
                     /locus_tag="VVN06_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU5561695.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            15645..16895
                     /locus_tag="VVN06_000075"
     CDS             15645..16895
                     /locus_tag="VVN06_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU5561696.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            16892..17434
                     /locus_tag="VVN06_000076"
     CDS             16892..17434
                     /locus_tag="VVN06_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU5561697.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            17580..19112
                     /gene="cls"
                     /locus_tag="VVN06_000077"
     CDS             17580..19112
                     /gene="cls"
                     /locus_tag="VVN06_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU5561698.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            19190..19333
                     /locus_tag="VVN06_000078"
     CDS             19190..19333
                     /locus_tag="VVN06_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561699.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            19370..20908
                     /locus_tag="VVN06_000079"
     CDS             19370..20908
                     /locus_tag="VVN06_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU5561700.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTNQGDQAGQLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            21028..23235
                     /gene="nrdD"
                     /locus_tag="VVN06_000080"
     CDS             21028..23235
                     /gene="nrdD"
                     /locus_tag="VVN06_000080"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU5561701.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            23267..23407
                     /locus_tag="VVN06_000081"
     CDS             23267..23407
                     /locus_tag="VVN06_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase"
                     /protein_id="HEU5561702.1"
                     /translation="MVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQV
                     TN"
     gene            23419..23559
                     /locus_tag="VVN06_000082"
     CDS             23419..23559
                     /locus_tag="VVN06_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561703.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            23603..24109
                     /locus_tag="VVN06_000083"
     CDS             23603..24109
                     /locus_tag="VVN06_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5561704.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            24102..24692
                     /gene="nrdG"
                     /locus_tag="VVN06_000084"
     CDS             24102..24692
                     /gene="nrdG"
                     /locus_tag="VVN06_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU5561705.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGILYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            24689..25306
                     /locus_tag="VVN06_000085"
     CDS             24689..25306
                     /locus_tag="VVN06_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU5561706.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            25572..25880
                     /gene="rpsJ"
                     /locus_tag="VVN06_000086"
     CDS             25572..25880
                     /gene="rpsJ"
                     /locus_tag="VVN06_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5561707.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            26097..26723
                     /gene="rplC"
                     /locus_tag="VVN06_000087"
     CDS             26097..26723
                     /gene="rplC"
                     /locus_tag="VVN06_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5561708.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            26748..27371
                     /gene="rplD"
                     /locus_tag="VVN06_000088"
     CDS             26748..27371
                     /gene="rplD"
                     /locus_tag="VVN06_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5561709.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            27371..27667
                     /locus_tag="VVN06_000089"
     CDS             27371..27667
                     /locus_tag="VVN06_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5561710.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            27685..28518
                     /gene="rplB"
                     /locus_tag="VVN06_000090"
     CDS             27685..28518
                     /gene="rplB"
                     /locus_tag="VVN06_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5561711.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            28622..28903
                     /gene="rpsS"
                     /locus_tag="VVN06_000091"
     CDS             28622..28903
                     /gene="rpsS"
                     /locus_tag="VVN06_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5561712.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            28915..29259
                     /gene="rplV"
                     /locus_tag="VVN06_000092"
     CDS             28915..29259
                     /gene="rplV"
                     /locus_tag="VVN06_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5561713.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            29272..29925
                     /gene="rpsC"
                     /locus_tag="VVN06_000093"
     CDS             29272..29925
                     /gene="rpsC"
                     /locus_tag="VVN06_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5561714.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            29929..30342
                     /gene="rplP"
                     /locus_tag="VVN06_000094"
     CDS             29929..30342
                     /gene="rplP"
                     /locus_tag="VVN06_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5561715.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            30352..30558
                     /locus_tag="VVN06_000095"
     CDS             30352..30558
                     /locus_tag="VVN06_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5561716.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            30583..30843
                     /gene="rpsQ"
                     /locus_tag="VVN06_000096"
     CDS             30583..30843
                     /gene="rpsQ"
                     /locus_tag="VVN06_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5561717.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            30869..31237
                     /gene="rplN"
                     /locus_tag="VVN06_000097"
     CDS             30869..31237
                     /gene="rplN"
                     /locus_tag="VVN06_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5561718.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            31315..31620
                     /gene="rplX"
                     /locus_tag="VVN06_000098"
     CDS             31315..31620
                     /gene="rplX"
                     /locus_tag="VVN06_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5561719.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            31644..32186
                     /gene="rplE"
                     /locus_tag="VVN06_000099"
     CDS             31644..32186
                     /gene="rplE"
                     /locus_tag="VVN06_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5561720.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            32204..32473
                     /gene="rpsN"
                     /locus_tag="VVN06_000100"
     CDS             32204..32473
                     /gene="rpsN"
                     /locus_tag="VVN06_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU5561721.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            32758..33156
                     /gene="rpsH"
                     /locus_tag="VVN06_000101"
     CDS             32758..33156
                     /gene="rpsH"
                     /locus_tag="VVN06_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5561722.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            33348..33884
                     /gene="rplF"
                     /locus_tag="VVN06_000102"
     CDS             33348..33884
                     /gene="rplF"
                     /locus_tag="VVN06_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5561723.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            33968..34324
                     /gene="rplR"
                     /locus_tag="VVN06_000103"
     CDS             33968..34324
                     /gene="rplR"
                     /locus_tag="VVN06_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5561724.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            34342..34836
                     /gene="rpsE"
                     /locus_tag="VVN06_000104"
     CDS             34342..34836
                     /gene="rpsE"
                     /locus_tag="VVN06_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU5561725.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            34808..35032
                     /gene="rpmD"
                     /locus_tag="VVN06_000105"
     CDS             34808..35032
                     /gene="rpmD"
                     /locus_tag="VVN06_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5561726.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            35177..35617
                     /gene="rplO"
                     /locus_tag="VVN06_000106"
     CDS             35177..35617
                     /gene="rplO"
                     /locus_tag="VVN06_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU5561727.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            35630..36940
                     /gene="secY"
                     /locus_tag="VVN06_000107"
     CDS             35630..36940
                     /gene="secY"
                     /locus_tag="VVN06_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5561728.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            37091..37729
                     /locus_tag="VVN06_000108"
     CDS             37091..37729
                     /locus_tag="VVN06_000108"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5561729.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            37846..38064
                     /gene="infA"
                     /locus_tag="VVN06_000109"
     CDS             37846..38064
                     /gene="infA"
                     /locus_tag="VVN06_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5561730.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            38089..38205
                     /gene="rpmJ"
                     /locus_tag="VVN06_000110"
     CDS             38089..38205
                     /gene="rpmJ"
                     /locus_tag="VVN06_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU5561731.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            38223..38588
                     /gene="rpsM"
                     /locus_tag="VVN06_000111"
     CDS             38223..38588
                     /gene="rpsM"
                     /locus_tag="VVN06_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5561732.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            38606..38989
                     /gene="rpsK"
                     /locus_tag="VVN06_000112"
     CDS             38606..38989
                     /gene="rpsK"
                     /locus_tag="VVN06_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5561733.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39032..39967
                     /locus_tag="VVN06_000113"
     CDS             39032..39967
                     /locus_tag="VVN06_000113"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5561734.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            39979..40365
                     /gene="rplQ"
                     /locus_tag="VVN06_000114"
     CDS             39979..40365
                     /gene="rplQ"
                     /locus_tag="VVN06_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5561735.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            40630..40896
                     /locus_tag="VVN06_000115"
     CDS             40630..40896
                     /locus_tag="VVN06_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5561736.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            40906..42243
                     /locus_tag="VVN06_000116"
     CDS             40906..42243
                     /locus_tag="VVN06_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU5561737.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            42487..43179
                     /locus_tag="VVN06_000117"
     CDS             42487..43179
                     /locus_tag="VVN06_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5561738.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(43282..44927)
                     /locus_tag="VVN06_000118"
                     /pseudo
     CDS             complement(43282..44927)
                     /locus_tag="VVN06_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(44917..46008)
                     /locus_tag="VVN06_000119"
     CDS             complement(44917..46008)
                     /locus_tag="VVN06_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561739.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLGLMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(46021..47142)
                     /locus_tag="VVN06_000120"
                     /pseudo
     CDS             complement(46021..47142)
                     /locus_tag="VVN06_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        14939 a         8718 c        10492 g        13065 t
ORIGIN      
        1 atcctgaggg ctattttttg ccattgttaa aaactccctt actttaattc tacaacctga
       61 ttataacaca aaaaagacaa agtacgaaaa aaataaaatt tttcaacaac agcgttgata
      121 aagtacgaaa aaaatagtac catgatttta tcaatcttga aaggagctat aagaatatgg
      181 cacaatggac gctaagagcg tgtcgagtaa atgcaggttt tactttgcgc caagtggcta
      241 agaaggtcaa caagaatttt cagactatct caaagtacga aaaagatagt acgctaatcc
      301 cttttgagct acttaaagag ttatcggaac tgtatcaggt aaaactggac gatatttttt
      361 taggagatag tacgaaaaaa atagaactaa atcaaaagga aatctaagaa aggagcaaac
      421 caatggaatt agtctacatg gacggtaaga aagagccgta tacactgagc agtattgttg
      481 cggagtgtgc agaagttaag cacagacatt tgaagatttt gctgaataaa catcgagagg
      541 acttcgagag tttcggaaag gtgcaattta aaatttcacc ttcagaaagt gggcaaaatg
      601 tacgggacta tattttgaat gagcaacaag caaccttgct gatcacttac ttacgaaata
      661 cagaacccgt aaaagagttt aagaagaacc tggtcaaagc attctttgaa atgcgtgagg
      721 agctttctaa gttccgtatg cagagggcgc tagaaaagcc aaaaagaaaa accttgcatg
      781 acagtataga gacatgggag gcaaaaccca agcacgcgca tagcaccatg aacaacctgc
      841 tactaaaagc agtaactgac atgaacgcta agcagttaag ggaagaacgt gggggctata
      901 acggtattga tagcttgacc agtgacgagc tggagcaata ccaggcattt gaggatatgg
      961 taatagccat gatcggcttg aatatgagtt accaagaaat caaggctatg gcattcagaa
     1021 ataaaaaaac acgccaaaga agcgcgtgag agcaacaaaa aaggcttagc aataaccaca
     1081 ccgcagagcc tttcacacta acactaaaac gaatttaaca aagcaggcaa gctattatca
     1141 aagaggtttt agtaaagatt tatacctaga ttatagcata tttagggcaa tttgaccata
     1201 cggagagcgc caactcttta aactggtact ttctcatgct ctcaatttgg cgactcagag
     1261 cgtgaggcta tgaattgcaa gaaaaactgt ataagaaaca accattaaaa acccccacac
     1321 tctcggtcgg caaacttctg agcgtgagga tatccagtat agtaaaaggc attaaaagct
     1381 cttagagcaa ttacacaaca tagaaacaaa ggtaaaaaac atgacagaaa caacatacga
     1441 gaatttaact agacatatag acagaattag cgcagaaatg cgagaaattg gcgaaaaaaa
     1501 tgggcttagt agactttcat tattggcaaa tcagacaaaa ttcatcaaag aaaatttaca
     1561 ccgtttactt tggattgaac ttcctgaatt gaatgacagc cataaaatcg aggcagtttc
     1621 taaaagcact acgggaatgt ttttccacgc tggtattttt gaaatggacg ctatgcgaca
     1681 agcattcttt aaacgccaag ctatgcactt ttttgacaac gaagcagagc aacaggcgta
     1741 tatagaacat gctgaaaagg agtatttaga ggctactata accttaaaag atattctttt
     1801 taactctaaa aatggaactc agagagtaaa taaaagttgt cttatagaga agtttgagga
     1861 ggcaatgcaa tgacactaaa cctagacaac atgacacaag cagagtttga tgaagtaatg
     1921 gctgaaatta aggaaaaacg ccccaacctc ttccagttta ttgctgattt tgtagatcga
     1981 aaagtaagca ctgaggaggt agacgaatac ctgaagatga aacatgaaaa acaagtggaa
     2041 tttatcaaga attatcaagc gagggcataa catgaatgag ttagatttga ccaatacaca
     2101 atcggtaatc tttatggtgg tattgattgg cttactgctt tacctaaacc accgagaccg
     2161 caaaaaaagc gcccaatttg agcgagaaaa ccaacagagg atagaaacac ctagcgagga
     2221 tttaagccct gattatgggc gttatatcca gcttgcaggg gtaagagttt acggaggaac
     2281 tgaatgagtc tatcagagaa tgacaaacga gtattaagac taatcaaggt aggggctgag
     2341 aactccataa caggggcaga aatcagcctt ataactaagc tggcggaaag aaccgtacaa
     2401 gatattatca gccgtctaat cacgcgtcat agtgttccta ttgttggggt tcgccatggg
     2461 acatttagag gatattttat cccagctaac aaagaggagc tactggacgg agcaaaggca
     2521 ttctataacc aagtacagga agaacagaaa cgcctaactg tccttttgaa tgctgattta
     2581 gagagttaca aagaaacctt gaaggaggtg ggcggatatg tttagcctaa gccgagaaag
     2641 tgagcaagac ctagcgcatg gcattctgga ggtagtagaa agatacctgg aagcgcgtga
     2701 tacagtcaag ccacgactga caggtttaat atcagcccag gaagtcatgg acgagttaga
     2761 tataaaatac atgacactcc aaaagtggga aaaagcaggg ctaagacggt atcagccacc
     2821 gctagaagat actaggaaaa tctattataa aatatctgac atctggaagt ttttgggggt
     2881 ggaaaatggc aaggtatagt attcatccgg cagataataa agggcattat cacaatatta
     2941 aattttacaa agagcgcagg ccaaccctag accagctaaa ggaacagcaa aggcttaaaa
     3001 agctgaagaa aaaacgcaaa aaggggcgat agtatgacaa tttatgaggc taaaggcttt
     3061 cagagtaatc ttgtctatcc atttgataaa attgagcctt tccagtatat tgaacgattc
     3121 aagcccttag tagttcctga aggggcagat attgaggaat acaagcgaac tcaagcgcct
     3181 tactgtataa gtggcaaagt gacgccagaa aagcatggca gttacaagcg aaacaactcc
     3241 agtctaatct atcgggattt gattttcctt gattatgacg atatacaggg gacgtctgag
     3301 ggctttataa aagccatttc tagcgcttta tttggctact cttacatctt atacccgact
     3361 atcaaacata gcttagaaaa gccccgtttt cgccttgtag tgaagcctga caacgtgatg
     3421 aatgaggcga cctataagca ggtagtgaaa gagattgctg acaagattgg actccccttt
     3481 gatatggcca gtttaacatg gtcacaactc caagggctac cagtcacaac aggcgaccca
     3541 gcaagttatc aaaagattgt agagcatgga cttgattatc cagtaccaca gacaacggct
     3601 gaaccagtca aacagaatgc ggcaagcctt ccatacactc caaggccaag cgggcagaaa
     3661 tctatgacca tgaggatcat tgacacgctt ttcaacggat ttggagacga gggagggcgc
     3721 aacgtagcac tgacacgatt tgtaggctta ctctttaata agtgggttga ttgtgaccta
     3781 gagacagcct atgaacttac aaagatagcc aatagcgtga cagttgaacc attgccgatt
     3841 gaggagctag accgcacttt tagcagtata gcacgggctg aatatagaaa gagaggttag
     3901 aaccatagaa ctagaagaat tagcaaacct aaaaagtgaa atcttggagg taatggagca
     3961 agaacaccct cctagaacga tgaaggagct tgaaaatcgt atctttaaag ctggagaaga
     4021 atggcgggca gaacatactg aaactaaggt aaatgagaac acaggggacg ttaccgaaaa
     4081 ggttgccatg ccccaaacct tcacagtagc taaaatactg agcgagattg tcacatttac
     4141 ttttatcagc aagagcaata tacctgatta tagcctactc tatatctatg atttagacga
     4201 gggtatctat accgctagta atgatctatt caatcttttg tgtaagacct ttgacgttag
     4261 aattaaaccc agagagtggc cacagattaa gttaatggtt agaacattgg caaagatacg
     4321 aaaaccttta gaaagtgcta acctcattcc cgtacagaac ggcattatca acttagaaac
     4381 taaggaacta ctccccttta gtcctaagta tgtaattaca agtaagataa gcacggccta
     4441 ccacgcgcct aaacgagtcc caacagatag agagggaaaa acttttgatg attggttgaa
     4501 ctcaatcgct tgtaatgata gtgaactggt aacactgttt tggcagatta tccttgaagc
     4561 tattgaccct aaccatactc gaaataagtt tgctatcttc tacggggacg gtaacaatgg
     4621 taaagggaca tttcagcgct tccttatcaa cctgataggg gaaagcaata tatcagcctt
     4681 aaagcctgca cagtttgctg aaaagcataa cttggagacc ttggtaggta aggtttgtaa
     4741 tattggggat gaagcaccta acgaatactt aaaaaatccg tctgatttaa tgagtatcac
     4801 tagtggcgac actgttctag tcaatccaaa aggacgccca gcctttgaag ctactttcaa
     4861 gttattcaat atcttttcag ggaattatat ccccaatggt ggcaacaaga caaaaggctg
     4921 gtatcgtaga attatgattg tcccttttaa tgctgacttt aacggggaga aggaaaaacc
     4981 ttggataaaa aatgagtttt tagcaaacaa ggaagtccta gaatatgccc tctacaaagc
     5041 tatcaatcaa gagccattta ctcactttat cgaacctaaa gcagttaaag gactcctaga
     5101 agaataccag gaagataatg actacttact ttcatgggtt aagcatgaat acatggaaaa
     5161 aggttggcat gagctggacg tagtacctgt ttttatcgtt acaagatcac taaagcacta
     5221 tgccgaagat atgggaatac ccaagcccaa tgtttacggg gctggtaaag aaactatcaa
     5281 gcacttacaa cagttgacac ctaatcggta ttctctaaga aagaaaagag tttctccaga
     5341 agattttgaa aaactagagg catgggacgt tgacaagata aaaatgcgga cacctcaaca
     5401 ctctatcact aaacaagaat aagtgtgacg ttcttttttt gcttaaaccc ttgataatac
     5461 tgattttctt gaaaaaagtg tgacgttgtg tgacgttctc aaaaaagaag gtcacacccc
     5521 ttgaagcctt gatatatcta actttagggg aataggtgtg acgttgtgac attctttcca
     5581 actcttatat atagagaaaa atacagtatt tatatatata ggaacgataa aacaaaagaa
     5641 cgtcacaggg tcacaggggt ggtgaaacac ctagagcctc aagggtttga acgtgtgacg
     5701 ttctttgtca caaaaggtca cagaaaacga aaaatattac ataatttacc ttaaaacaaa
     5761 gaaatgacaa caacatgaaa gttaaactat ttgaatatac tagacgatgg gaaaaagaag
     5821 actttgagaa agagatcgat gacttcatgg caactgttga agtgctggac gttaaatttt
     5881 ctggtacacc atctagtgaa tcctttaatt cggtggctat cgtattggta ttatacagat
     5941 aacaaaatgg ggtagatagt accgaccccc ttgcaatggt gtaactacta gtgacaccct
     6001 taaaaacaga aagaggaaaa acaacatgac attaaaaaca ttttcatcta aagccaaaac
     6061 atttactttc acttacgaat ttaaagacct agatacagct ctggtagcag gtcacgcgtt
     6121 actgggatat atgactggaa catattgcca gcctgtaatc tcattgacct acaaggataa
     6181 aggcacgcta gtagctgagt atgtggaaga tcacaagcta aacaagacct ttaagcgtat
     6241 ttgtgacagt tttaaagact accacaaaca accaggagag gctgaagcat ttgaagaacg
     6301 ttacaaacgg gagcgcgtgc tccaactcaa agagtcagag gactttgaga gtttactgaa
     6361 taaggtcaca gactacgagc tggaactatt agactatgct gaccgcctgc tttctgataa
     6421 gcctatccct atggactcta tgactgcttt tgctacgttg gaaatgctgg gcgatgaaag
     6481 tattagccta ctccaaaagt tggacgtaga gggcgaatat aaaggccttg ctggttatac
     6541 agagcatttg aagtagaata caggggagca atcccctttt tgctatcaag tcaatagttt
     6601 tgggtttttc catatagggg gaaataatga agcagttagc aatagaagca atcactaaac
     6661 caatgaagct gagaggaata tctaagggta tagccgagtt ggatggtcaa cgtttagaga
     6721 ttgatttgga tagtttaatg attgattttg gtggagagtc atttgaatta gacagaatag
     6781 caggaactaa gggaggtaat cgatattttt ttctttgccc tgattgtggt agacggtgca
     6841 ggttgcttta taaacggtat ttgtatttta gttgcggtac ttgtctagat atccacaagc
     6901 acaccctgaa ccgcagtaaa acggattgcc aatactactg ggagcttgca ctaaaagaag
     6961 ctagaaaggt agaaccaggg tggagtccta gacgtggcgg atatatgttt gatagttttc
     7021 ctgaaagacc aaaatacatg aaacagaaac gatattacaa gcattatcaa aagtttatta
     7081 actacaccag aaaaggggat agcttttggc tgaatggttt gagttctcta aggtaatttg
     7141 tagtagtgtg caatttacaa aacaaacaaa tactacatat tagacggttt gaatgttaaa
     7201 aaagctagat atatcaagga tttaattaag tatagcgttc caaaaaaagg gtgcagtaag
     7261 cgttccaaaa aagggtgagg ttgaatattt aaaaaaggct aagattaggc taaggtcaca
     7321 tacttcaaaa tagcaaggtt ttagcaaggt acagggcttt tttctagtga tagaacccca
     7381 aggttaccac aaggtatagc tactttttta gaaccataga acggtaaggt tacggtaagg
     7441 tatcgaagtt ttccaatggt aaggttttgg taaggtagca tatataaaaa gtacaaggtt
     7501 agtacaaggt tacatactta aaaatactaa ggttttacaa aggtagagag gtttaccgag
     7561 ggttagaaaa ggtgagattt tgcttaggta gtggcagtaa atatgcctga caaatggcag
     7621 ggttatgtgt gttgaactcc gagcgaacgc cgagaaagtg gctatatccc ccctatcatt
     7681 tatcaagtag ataagagcaa taaaaaccct ccttaacccc ctgagaaacg acaagcagat
     7741 aagagaagtt tttagccctc ttatttcgga tctactcctt gcagaatgac aagcagataa
     7801 ggacgacaat aagccttctt aactgcttac aaaacgatag ggagttaaga cgaatttaaa
     7861 agccccttat tttcgttttt ctccttgtca aacgggaagg aaataagaca ggaaataaca
     7921 tcgcttattt tgattttgct ccctgaaaaa ctgtaaggag ataaggagac ttattagctt
     7981 ccttaatttg aattttctcc ttacaaaatg acagggaaat aagaactaaa ataactcccc
     8041 ttattttcct accaaatgat aaggagataa gagcagttta aaagtctgtt attttggatt
     8101 tgttgcttac aaaatgataa gcagataagc aaagaaataa cttgccttaa ttgcttgcag
     8161 aatgacaagg aaataagaga gataaaaaca tcccttaatt gcttatcaaa tggtatggag
     8221 aatggacttg aacctagagc gaagctagag aaaatgaaaa aaagccagca cagggctgac
     8281 tcctttatga attattccat aaaactatta tatcacaaag gaggccgagg catgacacca
     8341 gagcaagtaa aagaaaaact agagggcgtt aagtggataa acaaggagat caaaggctta
     8401 tatttggaat tggaaaccct ggaaggtggc attatcaaaa aaccaacact aagccatagc
     8461 agggtgcaga caagtagaga gaataagaca gagaacaatc ttataagtgt tctgaagcta
     8521 aaagaggaca ctttacagag aattgagcga cttactgaag agagaatgaa aatatctgag
     8581 ctaatcgata aactggccaa tccgcttgag cgttctgttc taagactttt ttacttgaat
     8641 gatcttgtag tttcggaggt ggctgaagaa atagatagat caaatactac agtatacatt
     8701 ataaggcaaa aagctataga acacttaaca ggtattgtaa acggggattg atttaacctt
     8761 accacggaat taggagagta tgggggagtc ctaagccaca tattcgcccg tatttcgctt
     8821 tgtttcgtac tggacaacta aaacaccatg gataaactac aaaccctcag aaacgaatct
     8881 gggggctttt gtgaggtcat aatcttataa accacgcgct tcatggtata atatatctat
     8941 cagcaaccag caataaaaaa gcacgtttga ccgtgctagt ttcttgcctg ctgaactcgt
     9001 caaatcttag cccttttgtg gggctttttt gctccccttt ttgcgtactt tgggggagca
     9061 agtagtaaaa agtaatgtta gtaattttta ttaaaaagcg cgtgatataa gggttaggaa
     9121 gccctaagga aacataaaat aagcgattgt tgattatact acaaaatcgg ccttttgggt
     9181 aatagaatct cacttttttt ggtataatgg taagcaataa tggactagaa agaacaaaga
     9241 tgcaagataa aattgtcatt catggggcgc gtgcccataa tttaaaaaat attgatgtgg
     9301 agattccgcg agacaagttg gttgtcgtga ctggcttgtc aggttcaggg aagtccagtc
     9361 tggcttttga tactctctat gcggagggac aacgtcgcta tgtagagagt ttgtctgcct
     9421 acgctcgtca gttcttggga aatatggaaa aaccagatgt agatgccatt gatggtctca
     9481 gcccagctat ttccatcgac cagaaaacga ctagtaaaaa tcctcgctcg acggtgggaa
     9541 cgacgactga aatcaatgac tatctgcgtc tcctctacgc acgtgtgggg acgccttact
     9601 gtatcaacgg acatggagct atcaatgctt cttctgtgga gcaaatcgtt gataaggttt
     9661 tggagttacc tgaacgccag cgcttgcaga tcttggctcc tgtcatccgc aagaaaaaag
     9721 gccaacataa gagtgttatc gagaaggttc agaaagacgg gtatgttcgt gttcgtgtgg
     9781 atggggaagt ctatgatgtg accgaagtgc cagagttgtc taagagcaag caacacaata
     9841 tcgatgtcgt ggttgaccgt attgttatca aggagggcat tcgtagccgt ctctttgatt
     9901 ccattgaagc tgcccttcgt atcgcagaag gttatgtgat tatcgacacc atggacgact
     9961 ctgagttgtt gttctctgag cattatgcct gtccagtttg tggatttact gtcccagagt
    10021 tagagcctcg tctcttctct ttcaatgctc cctttggctc ttgtagtgag tgtgacggct
    10081 tggggattaa gctggaggtg gatactgatt tggtagtgcc agatgccagc aaaaccttac
    10141 atgagggagc gctggcacct tggaatccta tctcatccaa ctactatcca aacatgttag
    10201 agcaggccat gaaagtcttt ggagtggcta tggataagcc ttttgaggac ctgtcagaag
    10261 aagataagaa cttgattctc tacggctcag atggtaagga attccatttc cattatgaaa
    10321 atgaatttgg tggtgtgcgc gatatcgaca ttccgtttga gggagttatc aataatatca
    10381 aacgtcgtta ccatgaaacc aatagcgatt acactcgcac tcagatgcgt ctctacatga
    10441 atgagctgac ctgcggaacc tgtcaaggct atcgtctcaa tgaccaggcc ttgtctgtcc
    10501 gtgtgggcgg ccagcaaggg ccacatattg gagaaatctc agacctgtct atcgctgacc
    10561 acttggactt ggtgagccag ttaactttgt ctgaaaatga agccatcatt gctcgtccca
    10621 ttctcaagga aatcaaggat cgtttgacct tccttaataa tgtgggtctt aactatctaa
    10681 ccctgtcacg ttcagcagga accctttcag gtggggaaag tcagcgcatt cgtttggcaa
    10741 cccagattgg ttccaaccta tcaggtgtcc tctatatcct agacgagccg tcaatcggtc
    10801 ttcaccagag ggacaatgac cgcctgattg ccagtctgaa aaagatgcgt gacttgggca
    10861 atactcttat cgtggtggaa cacgacgaag ataccatgcg tgaagctgat tatctgattg
    10921 acgttggtcc tggtgccggt gtttttggtg gtgagattgt tgcagcaggt acacctaagc
    10981 aagtggctcg caacagcaag tctatcacag gtcagtactt gtcaggcaaa cgtgtcatcc
    11041 cagtaccaga agagcgccgt gtcggaaatg gtcgttttat tgaagtgaca ggagcgcgtg
    11101 agaacaactt gcaaaatgtc acggctcgct ttccactagg aaaatttatc gcagtgacag
    11161 gtgtgtcggg ttcagggaaa tcgactctaa tcaacagcat tctcaaaaaa gccattgccc
    11221 agaagctcaa ccgtaattca gacaaacctg gtaagtttaa gactattaca gggattgagc
    11281 atgtcgaccg tttgattgat attgaccaga gccctatcgg acggacgccg aggtctaacc
    11341 cagctaccta tacaggagtt tttgacgata tacgtgacct ttttgctcag acaaatgaag
    11401 ccaagattcg aggctacaag aaggggcgct tcagtttcaa cgtcaaggga ggtcgctgtg
    11461 aagcttgctc aggtgacggg attatcaaga ttgagatgca cttcttgccg gatgtttatg
    11521 tggcttgtga agtttgccac ggaacccgct ataacagtga aaccctagaa gttcactaca
    11581 aggaaaagaa tatttcgcag gtcttggata tgacggtcaa cgatgcggta gaatttttcc
    11641 aacacattcc gaaaattcaa cgcaaactcc agaccatcaa ggatgtaggt ctaggctatg
    11701 tgaccttggg acagccagct accacccttt ctgggggaga agcccaacgt atgaagttag
    11761 ctagcgaact ccacaaacgc tcgacaggaa aatctttcta cattctggat gagccgacga
    11821 cagggcttca tacagaggac attgctcgcc tgctcaaggt tttagctcgc tttgtagacg
    11881 acggcaatac agtcctcgtc atcgagcaca atctggatgt tatcaagacg gcagaccata
    11941 tcattgactt gggacctgag ggcggtgtcg gtggtggaac catcatcgta acaggaactc
    12001 cagaagaagt agctgccaac gaagccagct atacaggaca ctatttgaaa ggaaagttac
    12061 atcatgaata aacgcgtaca agcatttcta gctaaaatgc aagaaaaaga actagatggt
    12121 atcatcatca acaatcttaa aaacgtctat tatttgactg gtttttgggg ctcaaacgga
    12181 acagtcttta tcagccgtga ccgtcaggtc ttagtgacag actctcgcta tatcatcgca
    12241 gctaagcaag aaaccagtgg ttttgagatt gtggctgatc gtgatgaatt ggctgtcatt
    12301 gcaggaattg ttaaggacat gaacttgact cgtatcggtt ttgaagatga gatttcagtg
    12361 tcttattacc accgtatgca ggcagctttt gcaggtttga acttgcttcc acaaactcag
    12421 tttgtggaag gtcttcgtat gattaaggat gaggcagaga ttgcagcgat tcgcaaggct
    12481 tgttctatct cagaccaagc tttccgcgat gcgcttgact ttatcaaacc aggaaaaact
    12541 gaaattgaga ttgccaactt ccttgatttc cgcatgcgtg agttgggagc atctggctta
    12601 tcttttgata cgatcctagc tagcggtatc aattcttcta aaccccatgc ccatccaatg
    12661 cacaaaccag tggagttggg agaagccatt accatggact tcggctgtct ctatgaccac
    12721 tatgtcagtg atatgacccg gactatctat ctagggcatg ttagcgatga gcaggcagag
    12781 atttacaata cggttctaaa agctaaccaa gccttgattg accaagctaa ggcaggctta
    12841 ggtttccgtg actttgacaa aatccctcgt gatattatca ttgaggcagg ttatggtgac
    12901 tactttactc acggcattgg ccacggtatt ggtctggata tccatgagga accctacttt
    12961 agtcagactt ctacagaaac tattaagaca ggtatggcct tgaccgatga accaggtatc
    13021 tatatcgaag gcaaatatgg cgttcgtatc gaggatgata tcctgattac agagacaggt
    13081 tgtgaattat tgaccctagc tccaaaagag ttgatagtca tttagttgtt tgtcaagaaa
    13141 aaagtacgaa aatgacgaaa aaagtcaaaa aaaattaaaa ataggtcgca agtcggatgt
    13201 tttttatggt ataatagact aaactgatag taacatgtag cgaaaggggt aggtacatga
    13261 ttaaaattta tacagtctca agttgtacta gttgtaaaaa agcaaaaacc tggctcaatg
    13321 cccaccagtt aagttataaa gaacaaaacc ttggtaaaga aggaattacg agagaagaat
    13381 tactggatat tctaaccaaa acagataacg gaatagccag catcgtttcg tctaaaaatc
    13441 gctatgccaa agcccttgga gtggatattg aagatttgag tgtcaatgaa gttctcaatc
    13501 tgattatgga aacaccgaga attttaaaga gcccaatcct tgtagatgaa aaacgcctgc
    13561 aagttggcta caaggaagac gatattcgtg ccttcctacc acgctctgtc cgtaatgtag
    13621 aaaatgcaga agcacgtttg cgtgcagctc tataaacatc aaggctggga gcacttccca
    13681 gtcttattct attttaattt caaaaagaaa gaagaaagaa atgaaaaaaa tagttcttgt
    13741 tagtctagct ttcctttttg tcctggttgg ttgcggacag aaaaaagaaa ctggaccagc
    13801 tacaaaaaca gaaaaagata cgcttcagtc ggcattgcca gttattgaaa atgctgagaa
    13861 gaatacagtt gtaactaaga ctttggtctt gcccaagtca gatgatggta gccagcagac
    13921 acaaacaatt acttacaaag acaagacttt tttgagtcta gctatccaac aaaaacgtcc
    13981 agtctctgat gagttgaaga cttatattga ccaacatgga gtggaggaaa ctcaaaaagc
    14041 tcttcttgaa gcggaggaga aggataagtc tatcattgaa gctcgtaaat tggcaggttt
    14101 caaacttgaa acaaaactat tgagcgcaac ggaacttcaa acaacgacta gttttgattt
    14161 tcaagttctg gatgtcaaga aggcttccca gttggaacat ctgaagaata ttggtttgga
    14221 aaatcttttg aaaaatgaac caagcaaata tatttcagat agattggcaa atggcgcgac
    14281 agaacaatag aaaatccaaa taaatagact gcctgaattg tagaaggtaa ggaattatgc
    14341 tataatggta ctattctaag gaaagagggt gttagaatgg gatttactga agaaacagta
    14401 cgttttaaat tggacgattc caataaaaaa gaaattagcg aaactttgac agatgtttat
    14461 gcttcgttga acgataaggg ttacaaccca attaaccaaa tcgtaggtta cgtattgagt
    14521 ggagaccctg cctacgttcc tcgttataat aatgcacgaa atcaaatccg taagtatgag
    14581 cgtgatgaaa tcgttgagga attggttcgc tactacctca aaggacaagg agtcgatcta
    14641 taacctatga gaattatggg attggacgtc ggttcaaaaa cggtaggggt ggcgattagc
    14701 gatccgcttg gttttacagc tcaagggctt gaaatcatcc agataaatga agaacaaggc
    14761 caatttggtt ttgaccgcgt taaggaattg gttgatactt acaaggtgga acgatttgta
    14821 gtgggcttgc ctaaaaacat gaacaataca agtggaccgc gcgtagaagc tagtcaagcc
    14881 tacggagcaa agctagaaga gttttttggt ttaccagtag actatcagga tgaacgcttg
    14941 acaacagtgg ctgctgagcg catgttgatt gaacaagcag atatcagtcg caataagcgc
    15001 aagaaagtca ttgataagtt agcagctcag ctgattttac aaaattattt agatagaaaa
    15061 ttttaatata aaggagaggc tatgtcacac gatcataacc acgaccacga agaacgtgaa
    15121 ctaatcacac tagtagatga gcaaggaaat gaaaccttgt ttgaaatcct tttgacaatt
    15181 gatggaaaag aagaatttgg taaaaactat gttcttctag taccagttaa cgcagaagaa
    15241 gacgaagacg gacaagttga aatccaagct tactcattca ttgaaaatga agatggaaca
    15301 gaaggcgaat tgcaaccaat cccagaagac tcagaagacg aatggaacat gattgaagaa
    15361 gtcttcaaca gctttatgga ggagtgatac agtctgggag actgtatcag cccaactcca
    15421 gaaatgggga agagttggaa cgtccagtga acatttttac tccaactctt agaaattgag
    15481 aagagttgga acatctagtg gatgttttta gcccatattc acattcatag tagctagtta
    15541 ttagctaacc aggtaagagg tcgggacgaa aatcctggcc ttgtttttat tagtaaccat
    15601 actttaatgc gataattgat tggacttttg tcaaggagat gtgtatgttt gaagtagaag
    15661 aatggctcca tagtcggatt ggtttgaatt ttcgatcagg tttgggccga atgcagcaag
    15721 cggtggattt gttaggaaat cctgagcagt cttaccctat tatccacgta acagggacta
    15781 atgggaaagg atctaccatt gcttttatga gggaattatt tatggggcat ggcaaaaaag
    15841 ttgcgacctt tacctcccct catatcgtct ctatcaatga ccgaatctgc attaatgggc
    15901 aacctatagc agacgcagac tttatccgtt tgactgatca ggtcaaggag atggagaaaa
    15961 cgcttctgca aactcctgcc cagttgtcct tttttgaatt gctgaccttg gttgcttttc
    16021 tttattttag ggagcaagag gtggatttgg ttttattaga agtgggaatt ggtggcttac
    16081 ttgacacgac caatgtggta actggagagc ttgctgtcat cacctccatt gggcttgacc
    16141 atcaagaaac cttgggtgat agtctagaag caattgcaga gcagaaagct ggtattttca
    16201 aggctggtaa aaaggcagtg attgcgaaat tgcctccaga agctagactt gtctgtcaga
    16261 aaaaagccga atctttagct gttgaccttt atcaggcagg tcaagatttt tcagtgctga
    16321 atggtgattt ttcaagctct ttactaaatc tttcacagct gaacataggc ttagaaggag
    16381 cctatcagca ggagaatgcg gccttggcgt tgcaaacttt tcttcttttt atgggagaaa
    16441 gaaaggaagc tattgatgaa caggctgtaa gaaaggcctt ggaacagacc cattgggctg
    16501 gtcgcttgga gcgtattcgc ccacagattt acttggatgg tgctcataac ctccctgcct
    16561 tgactcgctt ggttgagttt atcaaagaaa aagagcagga aggctatcga cctcaaatcc
    16621 tctttggatc cttgaaacgt aaggattatc aagggatgtt gggttatctg actgaaaatt
    16681 tacctcaagt ggaactcaaa atgaccggct ttgactatca agggtctttg gacgagacag
    16741 atgtaacagg ttaccatgta attccctctt accgagaatt tatcagcagt ttcgaagaaa
    16801 gggcagacgc tcaagacttg ctgttcgtta cagggtctct ctattttatc tcagaagtac
    16861 ggggctacct gctggaccgt gagcagataa attgaccccc ttttttgaac ttgttatact
    16921 aggggaattg ctggttagag aaaatttctc taaattggta gcagaaagga aattcatcat
    16981 gaaattaaaa agattcacac tttctcttgc ttctctagca agttttagtc tcttagtagc
    17041 ttgttcacaa agagctcaac aggttcaaca gcctgttgct cagcaggtcc aacagcctgc
    17101 tcaacagaat accaatactg caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca
    17161 aaaccaacct gttgctcaac cgaccgatat tgatgggact tatactggtc aggatgacgg
    17221 agaccgtatc actttagtgg taactggaac gactggtaca tggactgagc tcgaatctga
    17281 cggggatcag aaagtcaaac aggttacatt tgattcagca aatcaacgca tgattattgg
    17341 cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc gtcgtagatg atatggatag
    17401 agacccatcg gaccaaatcg ttttaactaa ataagactat gcaagtagaa agggctggat
    17461 ttttcagcct ttttactttt acctagaaaa taatcataaa tacttgcctt ctaccgaata
    17521 cctgcgttat actagtatca attacttgtt tttagcattc aaactatcaa ggaggggata
    17581 tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg ctttagtctt tcgattatgc
    17641 tacttgaact ttgtcttgtt tttggtctct ttctttattt aggacgcatg gctcccattt
    17701 tatggattac tgtcctcatt ctactgagta tcatcacaat catttcgata gtcaaccgta
    17761 atacgactcc tgagaataag gtaacctggt tgttagtagc ctttgtgcca gtatttggtc
    17821 ccttgctcta tctgatgttt ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga
    17881 agaagctagg ctctatgcat ttccaagaag caaatagcca gctactaaaa gagaaattaa
    17941 aagaaagtga caaggcagct tatggagtca tcaagtcctt attgagtatg gataccaatg
    18001 ctgacatcta tgatcaaact gcctctacat tttttcctaa cggagaagct atgtggaaaa
    18061 agatggtaga agatcttaaa aaggctgaga aatttatttt cttggaatat tacattatag
    18121 aagaaggttt gatgtggaat cgcatactag atatactaga gcaaaaggta gctcagggtg
    18181 tagaggttaa gatgctctat gatgatatcg gctgtatggc tactttaaca ggagattatg
    18241 cacatcgact tcgtcagctg ggcatcgagg cccataaatt caataaagtt attcctcgtt
    18301 tgacagtggc ttataataac agagatcata gaaaaatatt gattgttgat ggtcagatag
    18361 cctatactgg tggggtcaat ctggcagatg agtacattaa ccacgtcgag agatttggtt
    18421 attggaagga tagtggaatt cgcttagacg gactagcagt aaaagctctg acacgcttat
    18481 ttttgaccac ttggtacatt aatcgaggag aaattagtga ttttgatcaa tatcatttag
    18541 aaaatcattc tatcccgagt gacggtttaa ccattccata cggaagtgga cccaagccaa
    18601 tttttcgagc gcaggtaggg aaaaaagttt atcagagttt aatcaatcaa gcaacagaat
    18661 cggtctatat tacgacacct tatttgatta tagattatga tttaacagag acaatcaaaa
    18721 atgcagctat gagaggggtc gatgttcgaa ttatcacccc ttacatacca gataagaagt
    18781 tcattcagtt agtcacgaga ggagcttatc ccgaccttct ttctgctggt gttcggattt
    18841 atgagtatag tccaggtttt attcatagta agcagatgtt ggtagacgaa gattttgcgg
    18901 tggtggggac aatcaatctc gactaccgga gcttggtaca ccattatgaa aatgcagtct
    18961 tactctataa aactccttct ataagggaaa tcgcccgaga ttttcgaaat atatttgcag
    19021 attctcagga agtctatcct cattctatca aaacgagctg gtatcaaaag cttgtaaaag
    19081 aaatcgccca actattcgcc cctatcttat aagaaatcta gaacttagga tacaaaccag
    19141 acctattttt cttgtctttt acgaatagaa agaaagagga ggggaattca tgctaactaa
    19201 ggaagaagtg aatgatatga ttgagttcaa acagacacat ttacaccgct ttcgggaaat
    19261 tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc
    19321 taggactttt tgatatacta agacagataa attgaacagg agaaacgata tgagtgtgta
    19381 tggtagagta gaagaagttc ataaggagaa tcgtgaacct ctagaatacc aaattgaaca
    19441 agaatcgcat catcgtgaat caagtcgtct tcctttggtg aaaattttac tatggagtac
    19501 gcttgtaacg gggataactc taggagtacc gctattactc gacttaatga gtgcacaaga
    19561 agtgcaggat ttttatgcag gttgggcgct tcatcagaca gggaagattt acagcgacta
    19621 ttatggaagt caaggtttgc tttattattt gctgacttac gtgagtcagg gcggattttt
    19681 ctttgccatc tttgagtggt tagccttggt agcaggcgga tttttccttt ttagatcagc
    19741 ggacaccttg acaaatcaag gagaccaagc tggacaactg gtgactattt tttacatgct
    19801 agttacaggt cttgcttttg gtggaggcta tgcgactctt ttagcgcttc ctttcttatt
    19861 cgcagccttt agtttagttg cggcttacct aagcaatcca agccatgata agggatttgt
    19921 acggattggg ctagctttgg caggcggatt tttctttgct cccttatcat cgctcctgtt
    19981 tattgctgta gtgagtttag gcttgttggt ctttaacctt gggcatagac gctttgcgca
    20041 tgggttttat cagtttcttg cagtggcttt aggtttttca cttgtctttt atccaactgc
    20101 ctactatagt gctgcaacag gaagttttgg ggatgcgatt agtggtattc gttatcctat
    20161 tgacagtatt cgctttgatt ttacttctaa aattttagag aatatgtttt tttatggctt
    20221 gttgtccctt ggtttgggat ttgtggtttg tatcttttta ggtctctttc aatccaaacc
    20281 ctttaaacta tacgtcattt cggttcctgc aagtcttgtg gtaattttag gtttgatttt
    20341 acttttcttt tcacaagagc ctctgcacgc ttcttatttg atggtcgtct tccctgtttt
    20401 cctactttta ttggtaacca atattaagag tcaacagagg gggcgtagtg ctagaagaag
    20461 ccgaagagaa acgccagtta gcctatggag tcgtttcttc aaaggaaatc tatatctgct
    20521 agtttttggg tttgtctatc ttttgtctgt tccttttttg atgaagtttg tcctttatcc
    20581 agtaccttat caagaacgta atcgtcttgc tgatttggta aaagaggaga caaatacgga
    20641 agatgctatc tatgcatggg atgatactgc gactctttat cgtaagagtg agcgcttgtc
    20701 gccatcggcg attttgtccc cgttgcacta tacagcaact gaggaaaatc gtaataagtt
    20761 acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg gtaaatgaca aggtggaagt
    20821 ctggtctgaa gtggaaacac tcttaaaaga aaattaccaa caagtaaaga ctgattactc
    20881 agagtttaaa gtctataaaa ttaaataacc aaatcaatat cttgtgtatt tttaaaaatt
    20941 ttaagatttt taacacaaga tattgatttt tctttttaga gtggtataat actttttaga
    21001 aagaacattt tagaaaagag catgcatatg attgcactag aagaaaaaat tacaattttg
    21061 ccaactctct tcgtcgagaa acgagatggg agacgtgttg tatttgatgt ggacaagatt
    21121 gacaaggctc tccacaaggc agctgacaag gttatggatg tgacacccct ggttgaaaaa
    21181 cgcctcaatg acctgactga gagaattatt acagaaattc atagtcgctt tccacaggga
    21241 attaagattt acgaaattca aaatatcgta gaacatgaac tccttgaagc caaagaatat
    21301 gcgctggctg aggagtatat tacttatcgg acacagaggg attttgagcg ctcaaaagcg
    21361 acggatatca actttagtat tcataaactt ctcaacaaag accagacagt tgtcaatgaa
    21421 aatgctaata aagacagtga tgtctttaac actcagcgtg atttgacagc agggattgtt
    21481 gggaaatcaa tcggactgca aatgcttcct aagcacgtag ccaatgccca ccaaaagggg
    21541 gatatccact atcacgattt ggactacagt ccctataccc ctatgaccaa ctgctgtttg
    21601 attgatttta agggtatgtt ggaaaatggt tttaagattg gaaatgcaga ggtagagagt
    21661 cccaagtcta tccagactgc gacagcacag atttctcaaa tcattgccaa cgttgcttct
    21721 agccagtacg gtggctgttc agctgaccgt atcgatgaaa ttttggcgcc ttatgcagag
    21781 aagaactatc aaaaacatct caaagatgca gaagagtggg tattgcctga aaaacgggaa
    21841 gattacgctt ggaagaaaac gcaaaaggac atctacgatg ccatgcaatc tcttgagtat
    21901 gaaatcaata ctctcttcac ttcaaatgga caaacacctt ttacttcgct aggttttggt
    21961 ctgggaacca gtcgttttga acgagaaatt caaaaagcta ttttaaacat tcgcatcaag
    22021 ggtcttggtt cagaacaccg tacggctatc tttcctaaac ttattttcac tctgaaaaga
    22081 ggccttaatt tagaagaagg aactcccaac tacgacatca agcagttggc tcttgagtgt
    22141 gcaaccaaac ggatgtatcc agatgtttta tcttacgata agattgttga cttgacagga
    22201 tccttcaagg tgcctatggg ttgccgttct ttcctccaag gatggaagga tgaaaatggt
    22261 gtagaagtca attcaggtcg catgaatctg ggtgttgtga cggttaatct gcctcgtatt
    22321 gctcttgagt ctgaaggtga tatgaataag ttctgggaaa tcttcaacga gcgaatgaat
    22381 atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta aagaggcgac accagcgaat
    22441 gctcctatcc tttatcagta cggtgctttt ggccatcgtc taggtaaaga agaaagtgtt
    22501 gaccagctct ttaagaatcg tcgtgcgacc gtttcgctgg gctatatcgg cttgtatgaa
    22561 gtagcgacag ttttctttgg taacagctgg gaaagtaatc cagatgctaa ggaattcacg
    22621 ctagacatca ttcacgatat gaaacgccgt gtagaagagt ggtcagacca atatggctac
    22681 catttctcta tctactcaac accatccgaa agtctgacag accgtttctg ccgactagat
    22741 atagacaagt ttggctctat tcctgatatc acagacaagg aatactacac caactctttc
    22801 cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat tggactttga gaaagtctat
    22861 ccagaagcag gtgcgtcagg tggtttcatc cattattgtg agtatccagt ccttcagcaa
    22921 aatccaaagg ccttggaagc tgtctgggat tatgcttatg accgtgtagg ctatctaggc
    22981 accaatactc cgattgaccg ttgctacaag tgtgactttg aaggggattt tgaaccaact
    23041 gagagagggt ttgcttgtcc aaactgtggc aatagcgacc ctaaaacagt agatgtggtg
    23101 aagcgaactt gtggctacct aggtaatcct caagcaagac cgatggtcaa cgggcgtcac
    23161 aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa cgattaaaat agctgggcat
    23221 caagtaacaa attagaaaga aatgaaatgg gaaaatatca acagatgtgg tgaagcgaac
    23281 ttgtggctac ctaggtaatc ctcaagcaag accgatggtc aacgggcgtc acaaggaaat
    23341 cgctgcgcgt gtcaaacata tgaatggttc aacgattaaa atagctgggc atcaagtaac
    23401 aaattagaaa gaaatgaaat gggaaaatat caactagacg ataaggggcg cgcacaagtg
    23461 acccgttatc acgagaaaca ctctaaaggt ggagctggta aaaaagaacg cttgcttagc
    23521 ttcagagaac aatttttaaa caagaacaag aaaaaataaa agtgagagcc agctctcgct
    23581 tttctcatag tgggaggtaa ggatggaatt acgcagacca agattagcgg ataagaaagc
    23641 tgttttagat atgatgacag agtttgaaaa gagccaatca gcccatgatg gaggattttg
    23701 ggatacagag aattttgtgt atgaagagtg gttggaaagc aatcaggaac aggaaatggg
    23761 gattaatctg cctgaaggat gggttcctgc aattcagtta gtggcttttt ctgagaaagg
    23821 tcaagcagtt ggatttctta atctccggtt gcgcctcagt aactttctac tagaagaagg
    23881 tgggcacatt ggctactcca tccgtccatc tgaaagaggc aagggttatg caaaagagac
    23941 tctccgtcag ggcttgcaag ttgctaagga aaagaacatc aagaaagctc tggtgacctg
    24001 tagcgtgaat aatcctgcta gcagagcagt cattctagca aatggtggaa tatttgagga
    24061 tgctcacaat ggagtcgagc gttattggat agaggtagcg aatgaataat ccaaaaccac
    24121 aagaatggaa aagcgaggaa cttagtcaag gtcgtatcat tgactacaag gcctttaact
    24181 ttgttgatgg cgaaggcgtg cgtaactctc tctatgtatc aggctgtatg tttcactgcg
    24241 agggatgtta taatgttgcg acttggtctt ttaatgctgg cattctctat acagcagaat
    24301 tagaagagca gattatggca gaccttgccc aaccctatgt tcaaggcttg actttgctgg
    24361 gaggggagcc ttttcttaat actggcattc tcttgcctct agttaaacgc atccgaaagg
    24421 aattgccaga caaggacatt tggtcctgga ccggctacac ttgggaagaa atgatgttgg
    24481 aaactccaga taaactggaa ctcttatcat tgattgacat tcttgtcgat ggacgatacg
    24541 atcgaactaa gagaaatctc atgctccagt ttcgaggttc gtccaaccaa cgaattatcg
    24601 atgtgcaaaa atcgctcaaa agtgggcaag tagtgatttg ggacaagctc aatgacggaa
    24661 aagaaagcta tgaacaggtg aagagagaat gaagaaaaaa gacttagtag accaactagt
    24721 ctcagagatc gagacgggga aagtcaggac actgggaata tacggtcatg gagcttcagg
    24781 taaatcaacc tttgcacagg aattgtacca agctttagat tctactacag taaatttgct
    24841 agagacagat ccttatatca cctcaggacg ccatctggta gtacccaagg acgcgccgaa
    24901 tcaaaaggtg acagccagtc tgccagtggc gcatgaactg gagagtttgc agagagatat
    24961 ccttgccttg caggcgggta tggatgtctt gacaattgaa gaaccttgga aggctagtga
    25021 ggtcttgtct ggagccaaac caattttgat tgtcgaaggg atgtctgttg gctttctacc
    25081 caaggaactc tttgaaaaaa ccatctgttt ctacacggat gaggagaccg aattaaagcg
    25141 acgccttgct agagatacga ctgtgagaaa tcgcgatgca tcctttatat tggctagcca
    25201 tcagatgaga cgggagcagt atctgcgata ctataaagaa actgagtcta aggcggatat
    25261 cttagtggac caatcagaag ataaatttga tgtcaagagg acttaactta tgtatagaag
    25321 aaatatagaa gaaaatccct aattttagaa agaaaaagta ggaaaacagt ttcatcccaa
    25381 aaaaacgaaa aaacctaaca aatcccttgc aatcgcaggg gatttgtgtt attctattat
    25441 ggtgctgtaa attacagcct tagttttgat gcaagaggtt gcgacacgct cggttgcatt
    25501 gccacgcaac accgcgtcgg ttttcttgtg gagctagcct attatcttaa atagacgaaa
    25561 aggagaaaaa gatggcaaac aaaaaaatcc gtatccgttt gaaagcttac gaacaccgta
    25621 cgcttgacac agcggctgca aaaatcgtag aatcagctac tcgtacaggt gcacaagttg
    25681 cgggtccaat cccacttcca actgaacgta gcctctacac aatcattcgt gcgactcaca
    25741 aatacaaaga ctctcgcgaa caatttgaaa tgcgtacaca caaacgtttg atcgatatcg
    25801 ttaacccaac tcaaaaaaca gttgatgcct tgatgaaatt ggatcttcca agtggtgtaa
    25861 acgtagaaat caaactttaa tctaaaatat aaaagagcag aggctggtgt ttcaatctaa
    25921 ttgaacacgg actaaactcg gtgtgaaaaa gataaacttc ctagtgtctg ctagacactg
    25981 cgtcagtttc ctattttcac tttgagtttg acgccctttg tatcttagac ttgagcataa
    26041 aaaacgctcg ttaaaaactt tttgaataaa aaatatagaa aaggaactat tttctcatga
    26101 caaaaggaat cttagggaaa aaagtgggaa tgactcaaat cttcactgaa gctggcgaat
    26161 tgatccctgt aacagttatt gaagcaactc caaacgttgt tcttcaagtt aaaactgttg
    26221 aaacagacgg atacaacgct atccaagttg gtttcgatga caaacgcgaa gtattgagca
    26281 acaaacctgc taaaggacat gtagcgaaag ctaacacggc tcctaagcgc ttcattcgtg
    26341 aattcaaaaa cgttgaaggc ttggaagttg gtgctgaaat tacagttgaa acattcgcag
    26401 ctggagacgt tgttgacgta actggtactt ctaaaggtaa aggtttccaa ggtgttatca
    26461 aacgccacgg acaatcacgt ggaccaatgg ctcacggttc tcgttaccac cgtcgtccag
    26521 gttctatggg acctgttgca cctaaccgcg tattcaaagg taaaaacctt gcagggcgta
    26581 tgggtggcga ccgcgtaaca attcaaaacc ttgaagttgt acaagttgtt ccagaaaaga
    26641 acgttatcct tatcaaaggt aacgtaccag gtgctaagaa atctcttatc actatcaaat
    26701 cagcagttaa agctggtaaa taataaagaa aggggaaatc agtcacaatg gcaaacgtaa
    26761 cattatttga ccaaactggt aaagaagctg gccaagttgt tcttagcgat gcagtatttg
    26821 gtatcgaacc aaatgaatca gttgtgtttg atgtaatcat cagccaacgc gcaagccttc
    26881 gtcaaggaac acacgctgtt aaaaaccgct ctgcagtatc aggtggtgga cgcaaaccat
    26941 ggcgtcaaaa aggaactgga cgtgctcgtc aaggttctat ccgctcacca caatggcgtg
    27001 gtggtggtgt tgtcttcgga ccaactccac gttcatacgg ctacaaactt ccacaaaaag
    27061 ttcgtcgcct agctcttaaa tcagtttact ctgaaaaagt tgctgaaaac aaattcgtag
    27121 ctgtagacgc tctttcattt acagctccaa aaactgctga atttgcaaaa gttcttgcag
    27181 cattgagcat cgattctaaa gttcttgtta tccttgaaga aggaaatgaa ttcgcagctc
    27241 tttcagctcg taaccttcca aacgtgaaag ttgcaactgc tacaactgca agtgttcttg
    27301 acatcgcaaa tagcgacaaa cttcttgtca cacaagcagc tatctctaaa atcgaggagg
    27361 ttcttgcata atgaatttgt atgatgttat caaaaaacct gtcatcactg aaagctcaat
    27421 ggctcaactt gaagcaggga aatatgtatt tgaagttgac actcgtgcac acaaactttt
    27481 gatcaagcaa gctgttgaag ctgctttcga aggtgttaaa gttgccaatg ttaacacaat
    27541 caacgtaaaa ccaaaagcta aacgtgttgg acgttacact ggttttacta acaaaactaa
    27601 aaaagctatc atcacactta cagctgattc taaagcaatc gagttgtttg ctgctgaagc
    27661 tgaataatct aaggaggaaa tatcgtggga attcgtgttt ataaaccaac aacaaacggt
    27721 cgccgtaata tgacttcttt ggatttcgct gaaatcacaa caagcactcc tgaaaaatca
    27781 ttgcttgttg cattgaagag caaggctggt cgtaacaaca acggtcgtat cacagttcgt
    27841 caccaaggtg gtggacacaa acgtttctac cgtttggttg acttcaaacg taataaagac
    27901 aacgttgaag cagttgttaa aacaatcgag tacgatccaa accgttctgc aaacatcgct
    27961 cttgtacact acactgacgg tgtgaaagca tacatcatcg ctccaaaagg tcttgaagta
    28021 ggtcaacgta tcgtttcagg tccagaagca gatatcaaag tcggaaacgc tcttccactt
    28081 gctaacatcc cagttggtac tttgattcac aacatcgagt tgaaaccagg tcgtggtggt
    28141 gaattggtac gtgctgctgg tgcatctgct caagtattgg gttctgaagg taaatatgtt
    28201 cttgttcgtc ttcaatcagg tgaagttcgt atgattcttg gaacttgccg tgctacagtt
    28261 ggtgttgtcg gaaacgaaca acatggactt gtaaaccttg gtaaagcagg acgtagccgt
    28321 tggaaaggta tccgcccaac agttcgtggt tctgtaatga accctaacga tcacccacac
    28381 ggtggtggtg aaggtaaagc accagttggt cgtaaagcac catctactcc atggggcaaa
    28441 cctgctcttg gtcttaaaac tcgtaacaag aaagcgaaat ctgacaaact tatcgttcgt
    28501 cgtcgcaacg agaaataata ttaaactagt cgcttaagca actagtaaat ccgccagctc
    28561 ggtagcgctc cataggagtg caagccgctg tggtacaaca tttaaaggag aaaatataaa
    28621 aatgggacgc agtcttaaaa aaggaccttt cgtcgatgag catttgatga aaaaagttga
    28681 agctcaagct aacgacgaaa agaaaaaagt tattaaaact tggtcacgtc gttcaacgat
    28741 cttcccaagt ttcattggtt acactattgc agtttatgac ggacgtaaac acgtacctgt
    28801 ttacatccaa gaagacatgg taggccacaa acttggtgaa tttgcaccaa ctcgtactta
    28861 caaaggtcac gctgcagacg acaagaaaac acgtagaaaa taaggagaac ataaatggca
    28921 gaaattactt cagctaaagc aatggctcgt acagtacgtg tttcacctcg taaatcacgt
    28981 cttgttcttg ataacatccg tggtaaaagc gtagccgatg caatcgcaat cttgacattc
    29041 actccaaaca aagctgctga aatcatcttg aaagttttga actcagctgt agctaacgct
    29101 gaaaacaact ttggtttgga taaagctaac ttggtagtat ctgaagcatt cgcaaacgaa
    29161 ggaccaacta tgaaacgttt ccgtccacgt gcgaaaggtt cagcttcacc aatcaacaaa
    29221 cgtacagctc acatcactgt agctgttgca gaaaaataag gaggtaaaat cgtgggtcaa
    29281 aaagtacatc caattggtat gcgtgtcggc atcatccgtg attgggatgc caaatggtat
    29341 gctgaaaaag aatacgcgga ttaccttcat gaagatcttg caatccgtaa attcgttcaa
    29401 aaagaacttg ctgacgcagc agtttcaact attgaaatcg aacgcgcagt aaacaaagtt
    29461 aacgtttcac ttcacactgc taaaccaggt atggttatcg gtaaaggtgg tgctaacgtt
    29521 gatgcactcc gtgcaaaact taacaaattg actggaaaac aagtacacat caacatcatc
    29581 gaaatcaaac aacctgattt ggatgctcac cttgtaggtg aaggaattgc tcgtcaattg
    29641 gagcaacgtg ttgctttccg tcgtgcacaa aaacaagcaa tccaacgtgc aatgcgtgct
    29701 ggagctaaag gaatcaaaac tcaagtatca ggtcgtttga acggtgcaga tatcgcccgt
    29761 gctgaaggat actctgaagg aactgttccg cttcacacac ttcgtgcaga tatcgattac
    29821 gcttgggaag aagcagatac tacatacggt aaacttggtg ttaaagtatg gatctaccgt
    29881 ggtgaagttc ttccagctcg taaaaacact aaaggaggta aataaccaat gttagtacct
    29941 aaacgtgtta aacaccgtcg tgagttccgt ggaaaaatgc gcggtgaagc aaaaggtgga
    30001 aaagaagtag cattcggtga atacggtctt caagctacaa ctagccactg gatcactaac
    30061 cgccaaatcg aagctgctcg tatcgccatg actcgttaca tgaaacgtgg tggtaaagtt
    30121 tggattaaaa tcttcccaca caaatcatac actgctaaag ctatcggtgt gcgtatggga
    30181 tctggtaaag gggcacctga aggttgggta gcaccagtta aacgtggtaa agtgatgttc
    30241 gaaatcgctg gtgtatctga agagattgca cgtgaagcgc ttcgacttgc tagccacaaa
    30301 ttgccagtta aatgtaaatt cgtaaaacgt gaagcagaat aaggagaagg catgaaactt
    30361 aatgaagtaa aagaatttgt taaagaactt cgtggtcttt ctcaagaaga actcgcgaag
    30421 cgcgaaaacg aattgaaaaa agaattgttt gaacttcgtt tccaagctgc tactggtcaa
    30481 ttggaacaaa cagctcgctt gaaagaagtt aaaaaacaaa tcgctcgtat caaaacagtt
    30541 caatctgaag cgaaataata gactagggaa ggagaaattt caatggaacg caataatcgt
    30601 aaagttcttg ttggacgtgt tgtatctgac aaaatggaca agacaatcac agttgtagtt
    30661 gaaacaaaac gtaaccaccc agtctatggt aaacgtatta actactctaa aaaatacaaa
    30721 gctcatgatg aaaacaatgt tgccaaagaa ggcgatatcg tacgtatcat ggaaactcgc
    30781 ccgctttcag ctacaaaacg tttccgtctt gtagaagttg ttgaagaagc ggtcatcatc
    30841 taatcaaacc tgaaaggaga aaactgaaat gattcaaaca gaaactcgtt tgaaagtcgc
    30901 agacaacagc ggtgctcgcg aaatcttgac tatcaaagtt cttggtggtt caggacgtaa
    30961 atttgcaaac atcggtgatg ttatcgtggc atctgtaaaa caagctactc ctggtggtgc
    31021 ggttaaaaaa ggtgacgttg ttaaagcagt tatcgttcgt actaaatcag gtgctcgtcg
    31081 tgctgatggt tcatacatca aatttgacga aaacgcagca gttatcatcc gtgaagacaa
    31141 aactcctcgc ggaacacgta tctttggccc agttgcacgt gaattgcgtg aaggtggctt
    31201 catgaagatc gtgtcacttg ctccagaagt actttaattt ttaggaacaa actagtcccc
    31261 tagcttcaag ctagggtgcc cttatgggcg taagaaaaat caaggagaaa cctaatgttt
    31321 gtaaaaaaag gcgacaaagt tcgcgtaatc gctggtaaag ataagggaac agaagctgtt
    31381 gtccttactg cccttccaaa agtaaacaaa gttatcgttg aaggtgttaa cattgttaag
    31441 aaacaccaac gtccaactaa cgagcttcct caaggtggta tcatcgagaa agaagcagct
    31501 atccacgtat caaacgttca agttttggac aaaaatggtg tagctggtcg tgttggatac
    31561 aaatttgtag acggtaaaaa agttcgctac aacaaaaaat caggcgaagt gcttgattaa
    31621 tcacgaagga aaggagaagt ataatggcaa atcgtttaaa agaaaaatat cttaatgaag
    31681 tagttcctgc tttgacagaa caattcaact actcatcagt gatggctgtg cctaaagtag
    31741 ataagattgt tttgaacatg ggtgttggtg aagctgtatc aaacgctaaa agccttgaaa
    31801 aagctgctga agaattggca cttatctcag gtcaaaaacc acttatcact aaagctaaaa
    31861 aatcaatcgc cggcttccgt cttcgtgaag gtgttgcgat cggtgcaaaa gttacccttc
    31921 gtggtgaacg tatgtacgaa ttcttggata aattggtatc agtttcactt ccgcgtgtac
    31981 gtgacttcca cggtgttcca acaaaatcat ttgatggacg cgggaactac acacttggtg
    32041 tgaaagaaca attaatcttc ccagaaatta acttcgatga cgttgacaaa actcgtggtc
    32101 ttgacatcgt tatcgtaaca actgctaaca ctgacgaaga gtcacgtgca ttgcttacag
    32161 gccttggaat gccttttgca aaataatata ggaggtaaat ctaatggcta aaaaatcaat
    32221 ggtagctaga gaggctaaac gccaaaaaat tgttgaccgt tatgctgaaa aacgtgctgc
    32281 attaaaggcg gcaggggact acgaaggttt atctaaatta cctcgcaacg cctcaccgac
    32341 tcgtttacat aatcgttgta gagttacggg gcgcccacat tcagtttacc gcaaatttgg
    32401 tctgagtcgt atcgcttttc gcgaacttgc gcataaaggt caaattcctg gtgtaacaaa
    32461 agcatcttgg taatttaaga tatcaagagc gtcaaaactc caagtaaaaa taggaaactt
    32521 gacgaagaaa ctaaagtttc taggaaagtt tatctttttc acacagagtt tagcccgggt
    32581 tcaattgggc ttgccaattt gaacacgagc tacagctttg gcaaaaaaga ccaatttgct
    32641 ttggagcatt gcttctgcat taaattgtct atttttgctc gtgctgttac gctctttgta
    32701 tcatgtatta actagcaagt gcaacttgca aactactagt aagaggagaa aaacaaaatg
    32761 gttatgactg acccaatcgc agacttccta actcgtattc gtaatgctaa ccaagctaaa
    32821 cacgaagtac ttgaagtacc tgcatcaaac atcaaaaaag ggattgctga aatccttaaa
    32881 cgcgaaggtt ttgtaaaaaa cgttgaaatc attgaagatg acaaacaagg cgtcatccgt
    32941 gtatttctta aatacggacc aaatggtgag aaagttatca ctaacttgaa acgtgtttct
    33001 aaaccaggac ttcgtgtcta caaaaaacgt gaagaccttc caaaagttct taacggactt
    33061 ggaattgcca tcctttcaac ttctgaaggt ttgcttactg ataaagaagc acgccaaaag
    33121 aatgttggtg gtgaggttat cgcttacgtt tggtaaaatc aagatacaaa gctcgtaaag
    33181 aacaaagcaa aattaggaag ttggagaagt ttgtttacaa acaagccaac ttatctattt
    33241 tgcacagttc ttagagcgtg ttcagttcag ctcttgaact aaataagtat ctgaaccccg
    33301 tgaaaactgg ccgttttggc ctgacaattt aacaggagaa aataaacatg tcacgtattg
    33361 gtaataaagt tatcgtgttg cctgctggtg ttgaactcgc taacaatgac aacgttgtaa
    33421 ctgtaaaagg acctaaagga gaacttactc gtgagttctc aaaagatatt gaaatccgtg
    33481 tggaaggtac tgaagtaact cttcaccgtc caaacgattc aaaagaaatg aaaactatcc
    33541 acggaactac tcgtgccctt ttgaacaaca tggttgttgg tgtatcagaa ggattcaaga
    33601 aagaacttga aatgcgtggg gttggttacc gtgcacagct tcaaggatct aaacttgttt
    33661 tggctgttgg taaatctcat ccagacgaag ttgaagctcc agaaggaatt acttttgaac
    33721 ttccaaaccc aacaacaatc gttgttagcg gaatttcaaa agaagtagtt ggtcaaacag
    33781 ctgcttacgt acgtagcctt cgttcaccag aaccatataa aggtaaaggt atccgttacg
    33841 ttggtgaatt cgttcgccgt aaagaaggta aaacaggtaa ataatgttga gtggttgatc
    33901 atcaaccacc aacctatttt ccaactttgt gcatagcaca cgatttaaaa ctaaagaggt
    33961 gaaaactgtg atttcaaaac cagataaaaa caaactccgc caaaaacgcc accgtcgcgt
    34021 tcgcggaaaa ctctctggaa ctgctgatcg cccacgtttg aacgtattcc gttctaatac
    34081 aggcatctac gctcaagtga ttgatgacgt agcgggtgta acgctcgcaa gtgcttcaac
    34141 tcttgataaa gaagtttcaa aaggaactaa aactgaacaa gccgttgctg tcggtaaact
    34201 cgttgcagaa cgtgcaaacg ctaaaggtat ttcagaagtg gtgttcgacc gcggtggata
    34261 tctatatcac ggacgtgtga aagctttggc tgatgcagct cgtgaaaacg gattgaaatt
    34321 ctaataggag gacactagaa aatggcattt aaagacaatg cagttgaatt agaagaacgc
    34381 gtagttgctg tcaaccgtgt tacaaaagtt gttaaaggtg gacgtcgtct tcgtttcgca
    34441 gctcttgttg ttgttggtga ccacaatggt cgcgtaggat ttggtactgg taaagctcaa
    34501 gaagttccag aagcaatccg taaagcagta gatgatgcta agaaaaactt gatcgaagtt
    34561 cctatggttg gaacaacaat cccacacgaa gttctttcag aattcggtgg agctaaagta
    34621 ttgttgaaac ctgctgtaga aggttctgga gttgccgctg gtggtgcagt tcgtgccgtt
    34681 gtggaattgg caggtgtggc agatattaca tctaaatcac ttggttctaa cactccaatc
    34741 aacattgttc gtgcaactgt tgaaggtttg aaacaattga aacgcgctga agaaattgct
    34801 gcccttcgtg gtatttcagt ttctgatttg gcataagaaa ggggataaaa tggctcaaat
    34861 taaaattact ttgactaagt ctccaatcgg acgcattcca tcacaacgta aaactgttgt
    34921 agcacttgga cttggcaaat tgaacagctc tgttattaaa gaagataacg ctgctatccg
    34981 tggtatgatc acagcagtat ctcacttagt aacagttgaa gaagtaaact aatgaagttt
    35041 taggggatgt gcactgtacc atcccctaaa actagatata gtcatctatg atgacatcgt
    35101 ataggcgagt tgatggggga gacaaccttt tctcccttat cggcgctagc attttacaaa
    35161 agaggagaaa ataaaaatga aacttcatga attgaaacct gcagaaggtt ctcgtaaagt
    35221 acgtaaccgc gttggtcgtg gtacttcatc aggtaacggt aaaacatctg gtcgtggtca
    35281 aaaaggtcaa aaagctcgta gcggtggcgg agttcgcctt ggttttgaag gtggacaaac
    35341 tccattgttc cgtcgtcttc caaaacgtgg attcactaac atcaacgcta aagaatacgc
    35401 aattgtgaac cttgaccaat tgaacgtctt tgaagatggt gctgaagtaa ctccagttgt
    35461 tcttatcgaa gcaggaattg ttaaagctga aaagtcaggt attaaaattc ttggtaacgg
    35521 tgagttgact aagaaattga ctgtgaaagc agctaaattc tctaaatcag ctgaagaagc
    35581 tatcactgct aaaggtggtt cagtagaagt catctaagag aggtgaccta tgttttttaa
    35641 attattaaga gaagctctta aagtcaagca ggttcgatca aaaattttat ttacaatttt
    35701 tatcgttttg gtctttcgta tcggaactag cattacagtt cctggtgtga atgccaatag
    35761 cttgaatgct ttaagtggat tatccttctt aaacatgttg agcttggtgt cggggaatgc
    35821 cctaaaaaac ttttcgattt ttgccctagg agttagtccc tatatcaccg cttctattgt
    35881 tgtccaactc ttgcaaatgg atattttacc caagtttgta gagtggggta aacaagggga
    35941 agtaggtcga agaaaattga atcaagctac tcgttatatt gctctagttc tcgcttttgt
    36001 gcaatctatc gggattacag ctggttttaa taccttggct ggagctcaat tgattaaaac
    36061 tgctttaact ccacaagttt ttctgacgat tggtatcatc ttaacagctg gtagtatgat
    36121 tgtcacttgg ttgggtgagc aaattacaga taagggatac ggaaacggtg tttccatgat
    36181 tatctttgcc gggattgttt cctcaattcc agagatgatt cagggcatct atgtggacta
    36241 ctttgtgaac gtcccaagta gccgtatcac ttcatctatc attttcgtaa tcattttgat
    36301 tattactgta ttgttgatta tttactttac aacttatgtt caacaagcag aatacaaaat
    36361 tccaatccaa tatactaagg ttgcacaagg tgctccatct agctcttacc ttccgttaaa
    36421 ggtaaatcct gctggagtta tccctgttat ctttgccagt tcgattactg cagcgcctgc
    36481 ggctattctt cagtttttga gtgccacagg tcatgattgg gcttgggtaa gggtagcaca
    36541 agagatgttg gcaactactt ctccaactgg tattgccatg tatgctttgt tgattattct
    36601 ctttacattc ttctatacgt ttgtacagat taatcctgaa aaagcagcag agagcctaca
    36661 aaagagtggt gcctatatcc atggagttcg tcctggtaaa ggtacagaag aatatatgtc
    36721 taaacttctt cgtcgtcttg caactgttgg ttccctcttc cttggtgtga tttccatttt
    36781 accgattgca gctaaagatg tatttggtct ttctgatgtt gttgcctttg gtggaacaag
    36841 tctcttgatc attatctcta caggtatcga aggaatcaag caattggaag gttacctatt
    36901 gaaacgtaag tatgttggtt tcatggacag aacagaataa aagtatttac tgaatcagta
    36961 aatactgagg gagtggaggt ttaaactctg acatttgtaa gagttggatc tcccctcttc
    37021 tattttgttt ttaaatcggg gtgaaaaaac tttttgcttc tatttaaaaa caaaataagg
    37081 agatcaaatt atgaatcttt tgattatggg cttacctggt gcaggtaagg gaactcaagc
    37141 agcaaaaatc gtagaacaat tccatgttgc acatatctca acaggtgata tgttccgcgc
    37201 tgcaatggca aatcaaactg aaatgggtgt tcttgctaag tcatatattg acaagggtga
    37261 attggttcct gacgaagtta caaatggaat cgtaaaagaa cgcctttcac aagatgatat
    37321 taaagaaaca ggattcttat tggatggtta cccacgtaca attgaacaag ctcatgcctt
    37381 ggacaaaaca ttggctgaac ttggcattga actagaaggt attatcaata ttgaagttaa
    37441 ccctgacagc ctcttggaac gtttgagtgg ccgtatcatc caccgcgtaa ctggagaaac
    37501 tttccacaag gtctttaacc caccagttga ctataaagaa gaagattact accaacgtga
    37561 agatgataag cctgagacag taaaacgtcg tttggatgtt aatattgctc aaggagaacc
    37621 aatcattgct cactaccgtg ccaaaggttt ggttcatgac atcgaaggta atcaagatat
    37681 caatgatgtc ttctcagata ttgaaaaagt attgacaaat ttgaaataaa gcgtttttca
    37741 cacttgcaaa aatccgctac aaatgttata ctgaaatagt ctgacttata attgttgtct
    37801 ctgtgtctag aggcatcgaa tcgaaattta tggaggtgct tttgcgtggc aaaagacgat
    37861 gtgattgaag ttgaaggcaa agtagttgat acaatgccga atgcaatgtt tacggttgaa
    37921 cttgaaaatg gacatcagat tttagcaaca gtttctggta aaattcgtaa aaactatatt
    37981 cgtattttag cgggagatcg tgttactgtc gaaatgagtc catatgactt gacacgtgga
    38041 cgtatcactt accgctttaa ataatcgaaa aacttggagg gataagaaat gaaagtaaga
    38101 ccatcggtca aaccaatttg cgaatactgt aaagttattc gtcgtaatgg tcgtgttatg
    38161 gtaatttgcc cagcaaatcc aaaacacaaa caacgtcaag gataagatag aaaggagaaa
    38221 acatggctcg tattgctgga gttgatattc caaatgacaa acgcgtagta atctcattga
    38281 cttatgttta tggtatcgga cttgcaacat ctaagaaaat tttggctgct gctggaatct
    38341 cagaagatgt tcgtgtacgt gatcttacat cagatcaaga agatgctatc cgtcgtgaag
    38401 tggatgcaat caaagttgaa ggtgaccttc gtcgtgaagt aaacttgaac atcaaacgtt
    38461 tgatggaaat cggttcatac cgtggtatcc gtcaccgtcg tggacttcct gtccgtggac
    38521 aaaatactaa aaacaacgct cgcactcgta aaggtaaagc tgttgcgatt gctggtaaga
    38581 aaaaataata taggaggtaa aagtcttggc taaaccaaca cgtaaacgtc gtgtgaaaaa
    38641 gaatatcgaa tctggtattg ctcatattca cgctacattt aataacacta ttgttatgat
    38701 tactgatgtg catggtaatg caattgcttg gtcatcagct ggtgctcttg gtttcaaagg
    38761 ttctcgtaaa tctacaccat tcgctgctca aatggcttct gaagctgctg ctaaatctgc
    38821 acaagaacac ggtcttaaat cagttgaagt tactgtaaaa ggtccaggtt ctggtcgtga
    38881 gtcagctatt cgtgcgcttg ctgccgctgg tcttgaagta acagcaattc gtgatgtgac
    38941 tccagtgcca cacaatggtg ctcgtcctcc aaaacgtcgc cgtgtataat catcgcatta
    39001 cactgctttt cgtttaagag ggagtaacta aatgatcgag tttgaaaaac caaatataac
    39061 aaaaattgat gaaaataaag attatggcaa gtttgtaatc gaaccacttg aacgtggcta
    39121 cggtacaact cttggtaact ctcttcgtcg tgtacttcta gcttctctac caggagcagc
    39181 tgtgacatct atcaacattg atggtgtgtt acatgagttt gacacagttc caggtgttcg
    39241 tgaagacgtg atgcaaatca ttctgaacat taagggaatt gcagtgaaat cgtacgttga
    39301 agacgaaaaa atcatcgaac tggatgttga aggtcctgct gaagtaacag ctggtgacat
    39361 tttgacagat agcgatattg aaattgtaaa tccagatcat tatctcttta caatcggtga
    39421 aggttcttct ctaaaagcga ctatgactgt taacagtggt cgtggatatg tacctgctga
    39481 cgaaaataaa aaggataatg caccagttgg aacacttgct gtagattcta tttatacacc
    39541 agttacaaaa gtcaactatc aagtggaacc tgctcgtgta ggtagcaatg atggtttcga
    39601 caaattaacc cttgaaatct tgacaaatgg aacaattatt ccagaagatg ctttagggct
    39661 ttcagcacgt attttgacag aacatcttga tttgtttaca aatcttactg agattgctaa
    39721 gtcaactgaa gtgatgaaag aagctgatac tgaatctgac gaccgtattt tagatcgtac
    39781 gattgaggaa ctggacttgt ctgtgcgttc atacaactgt ttaaaacgtg ccggtatcaa
    39841 tactgtgcat gatttgacag aaaaatctga agcagagatg atgaaagtac gaaatcttgg
    39901 acgcaagagt ttggaagaag tgaaactcaa actcattgat ttgggtcttg gattaaaaga
    39961 taaataaagg aggaatacat ggcttaccgt aaactaggac gcactagctc acaacgtaaa
    40021 gcaatgcttc gcgatttgac aactgacctt ttgatcaacg aatcaatcgt gacaactgaa
    40081 gctcgtgcta aagaaatccg taaaactgtt gaaaaaatga ttactctagg taaacgtggt
    40141 gatttgcatg cacgtcgtca agcagctgct ttcgtacgta atgaaatcgc atctgaaaac
    40201 tatgatgaag caactgataa gtacacttct actacagcac ttcaaaaatt gttctcagaa
    40261 atcgcacctc gttatgctga acgtaacggt ggatacactc gtatccttaa aactgaacca
    40321 cgtcgtggtg atgcagcgcc aatggcgatc atcgaattag tataaaatca tcaattttgt
    40381 tgagtgttat gatgatggag tcttgtgctc ttagtctagc tctggtctac cgctaggatt
    40441 tcggtcctag cgggaacact catcataagt tgggatagta gacgcttgtt tacgaaattg
    40501 tttttttctt aagaacaact tcgtaagcag gcgtttttga gtattttcgt tagaattatg
    40561 ctatactatt tgaaaagaat cctgtttaat gttaaggttt cttattttaa gaagaattgg
    40621 agtttactta tgaaagccat tataactgtt gttggtaaag ataaatctgg aattgttgca
    40681 ggtgtttctg gtaaaattgc agaattagga ttgaatattg acgatatctc tcaaactgta
    40741 ttggatgaat attttacgat gatggctgtt gtatctagtg atgaaaagca agattttacc
    40801 tatcttcgta atgaatttga agcttttggg caaactttga atgtaaaaat caatattcag
    40861 agtgcagcga ttttcgaagc tatgtataat atctaggagg tcatcatgga tattagacaa
    40921 gttactgaaa ccatcgccat gattgaggag caaaacttcg atattagaac cattaccatg
    40981 gggatttctc ttttggactg tatcgatcca aatatcaatc gtgctgcgga gaaaatctat
    41041 caaaaaatta cgacaaaggc ggctaattta gtagctgttg gtgatgaaat tgcggctgag
    41101 ttgggaattc ctatcgttaa taagcgtgta tcggtgacac ctatttctct gattggggca
    41161 gcgacagatg cgacggacta cgtggttctg gcaaaagcgc ttgataaggc tgcgaaagag
    41221 attggtgtgg actttattgg tggtttttct gccttagtac aaaaaggtta tcaaaaggga
    41281 gatgagattc tcatcaattc cattcctcgc gctttggctg agacggataa ggtctgctcg
    41341 tcagtcaata tcggctcaac caagtctggt attaatatga cggctgtggc agatatggga
    41401 cgaattatca aggaaacagc aaatctttca gatatgggag tggccaagtt ggttgtattc
    41461 gctaatgctg ttgaggacaa tccatttatg gcgggtgcct ttcatggtgt tggggaagca
    41521 gatgttatca tcaatgtcgg agtttctggt cctggtgtgg tgaaacgtgc tttggaaaaa
    41581 gttcgtggac agagctttga tgtagtagcc gaaacagtta agaaaactgc ctttaaaatc
    41641 actcgtatcg gtcaattggt tggtcaaatg gccagtgaga gactgggtgt ggagtttggt
    41701 attgtggact tgagtttggc accaacccct gcggttggag actctgtagc acgtgtcctt
    41761 gaggaaatgg ggctagaaac agttggcacg catggaacga cggctgcctt ggccctcttg
    41821 aacgaccaag ttaaaaaggg tggagtgatg gcctgcaacc aagtcggtgg tttatctggt
    41881 gcctttatcc ctgtttctga ggatgaagga atgattgctg cagtgcaaaa tggctctctt
    41941 aatttagaaa aactagaagc tatgacggct atctgttctg ttggattgga tatgattgcc
    42001 atcccagaag atacgcctgc tgaaaccatt gcggctatga ttgcggatga agcagcaatc
    42061 ggtgttatca acatgaaaac aacagctgtt cgtatcattc ccaaaggaag agaaggcgat
    42121 atgattgagt ttggtggtct attaggaact gcacctgtta tgaaggttaa tggggcttcg
    42181 tctgtcgact tcatctctcg cggtggacaa atcccagcac caattcatag ttttaaaaat
    42241 taagaaaata ggagaaattt taagttctat ttaagattag acgtgtatac tataatcatt
    42301 aaataaagac ctcctaatat tatttgaaac agataacact gaattagttt gaatttgatt
    42361 ttcatctaat atctttattt aatgaactcc taaacttttt cataataatc tccttcaaaa
    42421 gtcgcctgta tgggtggctt ttattttatc attcatgata taatagaagc aaacggagga
    42481 cggaaaatgg taaaagtacg attgtatttg gtacgtcatg gcaagaccat gtttaacacg
    42541 attggtcgcg cgcaaggttg gagcgatact cccttaactg ctgaaggtga acgagggatt
    42601 caagagttag gaatcggttt gcgagaatct gatctacagt ttgagcgcgc ttattcgagt
    42661 gattctggtc gtaccattca gaccatggga attatccttg aagaacttgg cttgcagggg
    42721 aaaatccctt atcgcatgga caagcgtatc agagaatggt gtttcggtag ttttgatgga
    42781 gcctatgatg gcgatctttt catgggcatt attcctcgta tctttaatgt ggaccacgtt
    42841 caccaattgt cttatgctga actggctgag ggcttggtag aggtcgatac agctggttgg
    42901 gctgaaggct gggaaaaact cagtggccga atcaaggaag gctttgaaat gattgcaaaa
    42961 gaaatggaag atcaaggtgg aggtaacgcc cttgttgtca gccatggaat gactattgga
    43021 accattgttt atctgattaa tggcatgcat ccgcatggtc tagataatgg tagcgtgacg
    43081 attcttgaat atgaggacgg tcagtttagg gttgaagttg tcggtgaccg tagttaccga
    43141 gagctaggac gtgagaagat ggaagaaggc tctatttaat cagtctagac ttgcttgcca
    43201 tgagctaggg atttgataag aatatcaaga taagaaaaaa cagccgaggg tactcctttc
    43261 ggctgttttt gatgtggaaa actaaagtgt aatgctattg cttttagaga ttttcataaa
    43321 caagagcaag gaacctactg ttagaacagt caggatagtt gacaaggctg cggctacacc
    43381 gtaatttcct ctgagaacct ctgtataaat agctacagtc attgttcttg ttttgacatt
    43441 gtagaggagg atagaagtag agagttttga aatcattgtg acccaagata agatggctcc
    43501 agaaatgata ccagatagca tcattggagt tgtaatctta gcaaaggtat tgagacgact
    43561 acttcctaag ctttcagcag cttcttcaat acttggtgct atttgttgta agctagcaac
    43621 agatgagcga atagtatacg gtaatcttct gacagataga gacataatca agatgaaagc
    43681 agtccctgta atcataagaa atccacttcc aaatagacca gtattgaagg aagaaatgaa
    43741 ggcaatccct agaacggttc ctggtacaat ataaggtacc atactgaggc tgtcaattaa
    43801 gtttgtaaac aaattccgtt ttctaacggc taggtaggag ataaatgtcg caaatagaac
    43861 aactagaact aaggcaatca aagggatacg aatggtattg aaaatagcag ctcccatacg
    43921 attgaaagct agcttgtaac tgtttggaga ataaccttta aaaaatacca tacctgatgt
    43981 ttttaggaaa gaggtataaa ttaagtagat ttgaggtaaa acagagataa agataattcc
    44041 gtagactgtt gcataaatgg cagtcatttt tccttttgta gtttttttag gctcaattgg
    44101 atggagcaga ttcatgctga aactgtagcg gtttgcaatg tgtttttgga taaggaaaat
    44161 tgccaaggca atgataatcg ccataattgc aaaagcagaa tttcctccaa cctcgctaat
    44221 aaattgggta taaatcagga cagggaaagt ccgatatcct tcgccaatca acataggcgt
    44281 tccaaagtct gagaatgctc tcataaatac aagcaaggag ctgctagtaa ggttggaact
    44341 aggagaggta aaacaaccgt tacgataggt ttaaatccga aggaccccat gctttcagct
    44401 gcttcaagta gagaattgtc aatactgttc attgttccag caacatatag aaataccagt
    44461 gggaatagtt gcagtgtaaa gacaagtata attcctttga atccataaat atcgatagct
    44521 ggaagataaa gggcatttgt caaaaattta gtgatgacct catttcgtcc cagcaagaga
    44581 atccaggagt aggctcctac gaaaggagct gacatggaag caatgataat caatatttgt
    44641 agaaatttct tccccttgaa gtcatacata gagaagagat aagctaatag ggttcctaca
    44701 actaaggaag tgacagtagc ggtaatggaa accctgaaac tgttgactag tgtctcagag
    44761 tagtaggctt tactaaagaa agtgacaaaa ttagctagtg agaattatcc ttcatgtata
    44821 agtgcttact tgagcacggt aacgatagga taaacgagaa agacaagata ggtaagaaag
    44881 atgaagaaag aggaggctgt ccaaatattt agttttttac gttccatggt tgactccttt
    44941 tatcaggttt tgggaaccat ctgcagaaaa gatgtttaat ttttgcgtat tgattcgtag
    45001 acgaatacga ttgccttttt gtagttcttc ttcaaaagtt gattcttcac taacttgaat
    45061 ttttgaggca aaacctgtct caatgaaata ctccgtattt agtccaagat agacgctatc
    45121 gctaatagtt ccttcaatat ctccagattc atctttgata aactcttcgg gacgaatgct
    45181 tacatgaata gcttgctcct caacctgatc aagagctagc attcgaaggg catagccatc
    45241 tgaaaagacg atataagcgc cgtcgctccg tttttcaaga ttggcaggga taatatttgt
    45301 gcgtccgata aaggttgcca caaactcatt agctggttta tgatagagtt cttttggtcg
    45361 gccgatttgt tggatcaccc catctttcat aacagcaatt tggtctgaaa tagccatggc
    45421 ttcttcttgg tcgtgggtta cataaacagt tgtaattccc acttcgtgtt ggatttctcg
    45481 gatggcttga cgcatatcca agcgaagttt ggcctccaga ttactaagtg gctcgtccat
    45541 gaggagaaca cttggattaa ccgctaaggc gcatgccaag gtgacacgtt gttgttgtcc
    45601 accactgagt ttatcgggct ttcgatccgc atattgagca atttgcatga gtccaagata
    45661 cttgttggtc tgttgaatca attcttcttt tggaaccttc ttttgcataa gaccaaaagc
    45721 aacgttgtct cggacagtca aatgtgggaa aatagcgtag ttttggaaaa ccagcccgat
    45781 attgcgtttg ctgggttcca tattattgat ttttgtatca tcgaagtaaa attctccatc
    45841 tttgatactg ttgaaacctg caatcatacg aagaagggtc gttttcccac atcctgaagc
    45901 tccaagaagg gtaaagagac ttccttttgg aattgtaatg ttcaaattct caataacagg
    45961 gacatcgtgg tagatttttt tggcgttaat aatttcgatc tcactcatag tgaacctctt
    46021 ttactgttta gattggatat ctgtaaagac ttcgttgtat ttcttaacga tatctgattt
    46081 attcttgatg acataatcat aatcttcagt gagtgttttg attttgtcaa ttggtttcat
    46141 gttttcgctt gttttagcat ttttacgaac aggacggtta gtagtggttg taccaagtgt
    46201 atcttgtact tcttgagaga taataaaatc gataaatttc ttggcatttt ccatattttt
    46261 agatttttta acgatagcag cactagcagg taggaagacg gttccttctt ttggatagac
    46321 taccttaatg ttagctccgt catttaagag tttaactgct ggatcttcat aagagagacc
    46381 aacagtcatt tctacatcag cgactgcttt atagacacca gatgaactta aaccgatttt
    46441 atagtatatt gaaactagaa tagtacacat ctgcttctaa aacattgtta gaaatcgatt
    46501 tgactgtcct gattaatttg tcctattctt atttcatttt actataccat caataagtgt
    46561 gaaaagatct tttacataag accaagcctt atcatctttg taaccacctt gagcttgtag
    46621 catatttgtt aattgagcaa aggcgctaga agagtttgct gggtcagcag ttgcgatttt
    46681 tccttttagt tcaggtttga gaaggtcgct atatccttcg atgttcatgc ctttagttaa
    46741 atcagggtgg acgattaaaa cactaccatc tagtgtataa ggagtagaga agccagttgt
    46801 gttttgatat tctttgataa cattatcatt ttcttttgaa atatagtttt caaagagttc
    46861 tccgtgggta gcatattgtg tataagaacc accaaagata acatcagcta caggaacttc
    46921 tttttctgac tctagttttt tgaaaagttc tccagtacca gcttgaatca gttctatttt
    46981 gataccatat ttttcttcaa aggcaggaat agttgctcca attaagcctt ctgagtttgg
    47041 tgaataaacg actagcgaac caccgtctcc tttatcagat gaactgtcat cggcagattc
    47101 attagaagaa caagcagcat aatacatcca tttctttttc atgatggata cctccgttgt
    47161 gttatttaag tttattttaa aacaatgtaa gcgtttttaa aacctacaat tcta
//
LOCUS       DAVHIU010000003        46273 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVHIU010000003 DAVHIU010000000
VERSION     DAVHIU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.003"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..213
                     /locus_tag="VVN06_000121"
     CDS             1..213
                     /locus_tag="VVN06_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU5561740.1"
                     /translation="MTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLPVHG
                     FCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            267..938
                     /locus_tag="VVN06_000122"
     CDS             267..938
                     /locus_tag="VVN06_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5561741.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(990..1187)
                     /locus_tag="VVN06_000123"
     CDS             complement(990..1187)
                     /locus_tag="VVN06_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5561742.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1180..2088
                     /locus_tag="VVN06_000124"
     CDS             1180..2088
                     /locus_tag="VVN06_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5561743.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2085..2546
                     /locus_tag="VVN06_000125"
     CDS             2085..2546
                     /locus_tag="VVN06_000125"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5561744.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2536..3423
                     /locus_tag="VVN06_000126"
     CDS             2536..3423
                     /locus_tag="VVN06_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5561745.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3426..5309
                     /gene="cbpE"
                     /locus_tag="VVN06_000127"
                     /gene_synonym="pce"
     CDS             3426..5309
                     /gene="cbpE"
                     /locus_tag="VVN06_000127"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU5561746.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5389..6519
                     /locus_tag="VVN06_000128"
     CDS             5389..6519
                     /locus_tag="VVN06_000128"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU5561747.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6529..7791
                     /locus_tag="VVN06_000129"
     CDS             6529..7791
                     /locus_tag="VVN06_000129"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU5561748.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7795..8592
                     /gene="proC"
                     /locus_tag="VVN06_000130"
     CDS             7795..8592
                     /gene="proC"
                     /locus_tag="VVN06_000130"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU5561749.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8812..9450
                     /locus_tag="VVN06_000131"
     CDS             8812..9450
                     /locus_tag="VVN06_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5561750.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK"
     gene            9447..10337
                     /locus_tag="VVN06_000132"
     CDS             9447..10337
                     /locus_tag="VVN06_000132"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU5561751.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10377..10694
                     /gene="yabA"
                     /locus_tag="VVN06_000133"
     CDS             10377..10694
                     /gene="yabA"
                     /locus_tag="VVN06_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU5561752.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10697..11575
                     /gene="rsmI"
                     /locus_tag="VVN06_000134"
     CDS             10697..11575
                     /gene="rsmI"
                     /locus_tag="VVN06_000134"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5561753.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQHGMKKNQAIKEVAKYYQWNKS
                     QLYAAYHEWEENQEND"
     gene            11762..13096
                     /gene="trmFO"
                     /locus_tag="VVN06_000135"
     CDS             11762..13096
                     /gene="trmFO"
                     /locus_tag="VVN06_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU5561754.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            13180..13917
                     /locus_tag="VVN06_000136"
     CDS             13180..13917
                     /locus_tag="VVN06_000136"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5561755.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            13926..14483
                     /gene="frr"
                     /locus_tag="VVN06_000137"
     CDS             13926..14483
                     /gene="frr"
                     /locus_tag="VVN06_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5561756.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            14543..15397
                     /gene="cvfB"
                     /locus_tag="VVN06_000138"
     CDS             14543..15397
                     /gene="cvfB"
                     /locus_tag="VVN06_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU5561757.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDLTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            15406..15621
                     /locus_tag="VVN06_000139"
     CDS             15406..15621
                     /locus_tag="VVN06_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU5561758.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            15707..16675
                     /locus_tag="VVN06_000140"
     CDS             15707..16675
                     /locus_tag="VVN06_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5561759.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEETACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(16653..16832)
                     /locus_tag="VVN06_000141"
     CDS             complement(16653..16832)
                     /locus_tag="VVN06_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561760.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            16868..17368
                     /locus_tag="VVN06_000142"
     CDS             16868..17368
                     /locus_tag="VVN06_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5561761.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNCRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            17371..17631
                     /locus_tag="VVN06_000143"
                     /pseudo
     CDS             17371..17631
                     /locus_tag="VVN06_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(17998..18324)
                     /locus_tag="VVN06_000144"
     CDS             complement(17998..18324)
                     /locus_tag="VVN06_000144"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561762.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(18270..18683)
                     /locus_tag="VVN06_000145"
     CDS             complement(18270..18683)
                     /locus_tag="VVN06_000145"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5561763.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(18684..19109)
                     /locus_tag="VVN06_000146"
     CDS             complement(18684..19109)
                     /locus_tag="VVN06_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5561764.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVADRMAV"
     gene            19286..19855
                     /locus_tag="VVN06_000147"
     CDS             19286..19855
                     /locus_tag="VVN06_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5561765.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            19923..20573
                     /locus_tag="VVN06_000148"
     CDS             19923..20573
                     /locus_tag="VVN06_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU5561766.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            20557..22797
                     /locus_tag="VVN06_000149"
     CDS             20557..22797
                     /locus_tag="VVN06_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU5561767.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GYLNTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLIGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            22939..23163
                     /locus_tag="VVN06_000150"
     CDS             22939..23163
                     /locus_tag="VVN06_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561768.1"
                     /translation="MIIGLFQSSISAVTATKSYKYDWNTVLEYSINYHDHQYAWIPEW
                     SRYYSYSEYKVGGGWNYARYEVINYYSGGY"
     gene            23196..23783
                     /locus_tag="VVN06_000151"
     CDS             23196..23783
                     /locus_tag="VVN06_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561769.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            23787..24968
                     /locus_tag="VVN06_000152"
     CDS             23787..24968
                     /locus_tag="VVN06_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561770.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    25128..25259
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            25275..25805
                     /gene="infC"
                     /locus_tag="VVN06_000153"
     CDS             25275..25805
                     /gene="infC"
                     /locus_tag="VVN06_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5561771.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            25838..26038
                     /gene="rpmI"
                     /locus_tag="VVN06_000154"
     CDS             25838..26038
                     /gene="rpmI"
                     /locus_tag="VVN06_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5561772.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            26090..26449
                     /gene="rplT"
                     /locus_tag="VVN06_000155"
     CDS             26090..26449
                     /gene="rplT"
                     /locus_tag="VVN06_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5561773.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            26507..26887
                     /locus_tag="VVN06_000156"
     CDS             26507..26887
                     /locus_tag="VVN06_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5561774.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            27137..27937
                     /locus_tag="VVN06_000157"
     CDS             27137..27937
                     /locus_tag="VVN06_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU5561775.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            27948..28886
                     /locus_tag="VVN06_000158"
     CDS             27948..28886
                     /locus_tag="VVN06_000158"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU5561776.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSTEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            28928..31093
                     /gene="lytB"
                     /locus_tag="VVN06_000159"
     CDS             28928..31093
                     /gene="lytB"
                     /locus_tag="VVN06_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU5561777.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYNSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVAFHLSDMEVGKKYYSADGLHFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(31154..32809)
                     /gene="pavA"
                     /locus_tag="VVN06_000160"
     CDS             complement(31154..32809)
                     /gene="pavA"
                     /locus_tag="VVN06_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU5561778.1"
                     /translation="MSFDGFFLHHIVEELRNELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            32914..33411
                     /gene="ybeY"
                     /locus_tag="VVN06_000161"
     CDS             32914..33411
                     /gene="ybeY"
                     /locus_tag="VVN06_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5561779.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            33392..33787
                     /locus_tag="VVN06_000162"
     CDS             33392..33787
                     /locus_tag="VVN06_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU5561780.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            33804..34703
                     /gene="era"
                     /locus_tag="VVN06_000163"
     CDS             33804..34703
                     /gene="era"
                     /locus_tag="VVN06_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5561781.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            34752..35576
                     /gene="mutM"
                     /locus_tag="VVN06_000164"
     CDS             34752..35576
                     /gene="mutM"
                     /locus_tag="VVN06_000164"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU5561782.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            35576..36181
                     /locus_tag="VVN06_000165"
     CDS             35576..36181
                     /locus_tag="VVN06_000165"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5561783.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            36168..37367
                     /locus_tag="VVN06_000166"
     CDS             36168..37367
                     /locus_tag="VVN06_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU5561784.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEVFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            37367..37516
                     /gene="rpmG"
                     /locus_tag="VVN06_000167"
     CDS             37367..37516
                     /gene="rpmG"
                     /locus_tag="VVN06_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5561785.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            37556..37789
                     /gene="secG"
                     /locus_tag="VVN06_000168"
     CDS             37556..37789
                     /gene="secG"
                     /locus_tag="VVN06_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5561786.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            37891..40245
                     /gene="rnr"
                     /locus_tag="VVN06_000169"
     CDS             37891..40245
                     /gene="rnr"
                     /locus_tag="VVN06_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU5561787.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            40208..40675
                     /gene="smpB"
                     /locus_tag="VVN06_000170"
     CDS             40208..40675
                     /gene="smpB"
                     /locus_tag="VVN06_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5561788.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            40691..41551
                     /gene="tehB"
                     /locus_tag="VVN06_000171"
     CDS             40691..41551
                     /gene="tehB"
                     /locus_tag="VVN06_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU5561789.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTKPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDIPVDLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            41624..42577
                     /gene="coiA"
                     /locus_tag="VVN06_000172"
     CDS             41624..42577
                     /gene="coiA"
                     /locus_tag="VVN06_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU5561790.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            42596..44407
                     /gene="pepF"
                     /locus_tag="VVN06_000173"
     CDS             42596..44407
                     /gene="pepF"
                     /locus_tag="VVN06_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU5561791.1"
                     /translation="MVLQRNERNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVA
                     QYEGHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLY
                     SQLDQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELL
                     AGAGEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAY
                     QALYATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAA
                     VRKHLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGE
                     DYLSRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETG
                     HSMHSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLD
                     GFRGTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQY
                     EWARIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVM
                     RKAGVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            44409..45122
                     /locus_tag="VVN06_000174"
     CDS             44409..45122
                     /locus_tag="VVN06_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU5561792.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFYATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            45189..46130
                     /gene="prsA"
                     /locus_tag="VVN06_000175"
     CDS             45189..46130
                     /gene="prsA"
                     /locus_tag="VVN06_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU5561793.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        13875 a         8479 c        10613 g        13306 t
ORIGIN      
        1 atgacaaaaa atataaatct aacaaatgaa gaattagagc tgatacaagg tggagcagat
       61 ccatatggta aaaatcctaa tggtaggtac gattgggaaa tagaaccagt attaactctg
      121 ccggttcatg gattttgtcc cagaggcacc tatgatttag gatatattgg aggaggcaat
      181 catctttgca aaggaagtgc tgcgagattt taagtaaaat ttattagtaa tatgaagaaa
      241 caagtggaga aagcagagaa tttaatatga aaaaacgggc tattcaaatt ttactagcat
      301 tgtccttaat tttttacaaa tcaacttggt tttggagagt tttcaatcac ctcgcaaagc
      361 cctatctacc agcaagtcgt gaattttttc agattctgct tttgatggag agcggagttc
      421 ttttcttagc ggtcatctat ctactggttt ttgcaggaaa gaaaattttt catttcaagt
      481 ggcagctgag gtacttcatc taccttttac tgggctacat catttcatat atgtctgact
      541 tcctcttttc gtatttcata tccctgtctt caaatcagat ttctttgaat gaaacggtag
      601 aaatgatggg gagacaggag ttcccttatt tcttgctcat cgtttgcttc atcgccccta
      661 ttgctgagga attgatttat cgaggtgtgc ttatgacaac ctttttcaaa aactcacctt
      721 ggtacggaga tgttttgctt tctgctatta ttttcggtta tattcatatc aattttgctt
      781 taactcctct tgcttttttc atttatgcta gtggaggtct tattttagct ctattgtatc
      841 gcatgactaa aaatctctac tatccaatac tagttcatat tctcatcaat atcactgcct
      901 tctgggatgt ttggttactc ctattttcag gaagttagct tactaaaaaa atgtcggaat
      961 tttccggcat tttctttttt cacaaatagt caacgttttt ctttccgata ctgaagtggt
     1021 gtgtagccac ttattttttt gaattgattt tgaaaataag attggcgtga gaaaggcaga
     1081 tagtgaagat agttaagaag aataggatgt tcttttttcc tttttggaaa acttctaaaa
     1141 tatggtataa tgaaaagata aagaagttgg gggtagaaga tgaacattca acaattacgc
     1201 tatgttgtgg ctattgccaa tagtggtact tttcgtgaag ctgctgaaaa gatgtatgtt
     1261 agtcagccga gtctgtctat ttctgttcgt gatttggaaa aagagttggg ctttaagatt
     1321 ttccgtcgga ccagctcagg gactttcttg acccgtcgtg ggatggaatt ttatgaaaaa
     1381 gcgcaagaat tggttaaagg atttgatatt tttcaaaatc agtatgccaa tcctgaagaa
     1441 gaaaaagatg aattttccgt tgctagccag cactatgact tcttaccacc aactattacg
     1501 gccttttcag agcgctatcc tgactataag aacttccgta tttttgaatc aactactgtt
     1561 caaatattag atgaagtggc gcaagggcat agtgagattg ggattatcta cctcaacaat
     1621 caaaataaaa aggggattat gcaacgggtt gaaaagttag gtctggaggt catcgaattg
     1681 attcctttcc atacccatat ttatctctgt gagggtcatc ctttagctca gaaagaggaa
     1741 ttagtcatgg aggatttagc ggatttacca acggttcgtt tcactcaaga gaaagacgag
     1801 tacctttatt attcagagaa ctttgtcgat accagcgcta gctcacagat gtttaatgtg
     1861 acagaccgtg ccaccttgaa tggtattttg gagcggacgg acgcctatgc gacaggttct
     1921 ggatttttag atagtgacag tgttaatggc attacagtta ttcgtctcaa ggataaccta
     1981 gataaccgca tggtctatgt taaacgtgaa gaagtggagc ttagtcaagc tgggactctc
     2041 ttcgtagaag tcatgcaaga atattttgat caaaagagga aatcatgaaa aaaagagcaa
     2101 tagtggcagt cattgtactg cttttaattg ggctggatca gttggtcaaa tcctatatcg
     2161 tccagcagat tccactgggt gaagtgcgct cctggattcc caatttcgtt agcttgacct
     2221 acctgcaaaa tcgaggtgca gccttttcta tcttacaaga tcagcagctg ttattcgctg
     2281 tcattactct ggttgtcgtg ataggtgcca tttggtattt acataaacac atggaggact
     2341 cattctggat ggtcttgggt ttgactctaa taatcgcggg tggtcttgga aactttattg
     2401 acagggtcag tcagggcttt gttgtggata tgttccacct tgactttatc aactttgcaa
     2461 ttttcaatgt ggcagatagc tatctgacgg ttggagtgat tattttattg attgcaatgc
     2521 taaaagagga aataaatgga aattaaaatt gaaactggtg gtctgcgttt ggataaggct
     2581 ttgtcagatt tgtcagaatt atcacgtagt ctcgcgaatg aacaaattaa atcaggccag
     2641 gtcttggtca atggtcaagt caagaaagct aaatacacag tccaagaggg tgatgtcgtc
     2701 acttaccatg tgccagaacc agaggtatta gagtatgtgg ctgaggatct tccgctagaa
     2761 atagtctacc aagatgagga tgtggctgtc gttaacaaac ctcagggaat ggttgtgcac
     2821 ccgagtgctg gtcataccag tggaacccta gtaaatgccc tcatgtatca tattaaggac
     2881 ttgtcgggta tcaatggggt tctgcgtcca gggattgttc accgtattga taaggatacg
     2941 tcaggtcttc tcatgattgc taaaaacgat gatgcgcatc tagcacttgc ccaagaactc
     3001 aaggataaaa agtctctccg caaatattgg gcgattgttc atggaaatct gcctaatgat
     3061 cgtggtgtaa ttgaagcgcc gattggccgg agtgaaaaag accgtaagaa acaggctgta
     3121 actgctaaag ggaagcctgc agtgacgcgt tttcacgtct tggaacgctt tggcgattat
     3181 agcttagtag agttgcaact ggagacaggg cgcactcatc aaatccgtgt ccacatggct
     3241 tatatcggcc attcagtcgc tggtgatgag gtctatggtc ctcgcaagac tttgaaagga
     3301 catggacaat ttcttcatgc caagacttta ggttttactc atccgagaac aggtaagacc
     3361 ttggaattta aagcagatat cccagagatt tttaaggaaa ccttggagag attgagaaag
     3421 taagaatgaa aaagaaatta actagtttag cacttgtagg cgctttttta ggtttgtcat
     3481 ggtatgggaa tgttcaggct caagaaagtt caggaaataa aatccacttt atcaatgttc
     3541 aagaaggtgg cagtgatgcg attattcttg aaagcaatgg acattttgcc atggtggata
     3601 caggagaaga ttatgatttc ccagatggaa gtgattctcg ctatccatgg agagaaggaa
     3661 ttgaaacgtc ttataagcat gttctaacag accgtgtctt tcgtcgtttg aaggaattgg
     3721 gtgtccaaaa acttgatttt attttggtga cccataccca cagtgatcat attggaaatg
     3781 ttgatgaatt actgtctacc tatccagttg accgagtcta tcttaaaaaa tatagtgata
     3841 atcgtattac taattctgaa cgtctatggg ataatctgta cggttatgat aaggttttac
     3901 agactgcttc agaaaaaggt gtttcagtta ttcaaaatat cacacaaggg gatgctcatt
     3961 ttcagtttgg ggacatggat attgagctct ataactatga aaatgaaacc gattcatcgg
     4021 gtgaattaaa gaaaatttgg gatgacaatt ccaattcctt gattagcgtg gtgaaagtca
     4081 atggcaagaa aatttacctt gggggcgatt tagataatgt tcatggagca gaagacaagt
     4141 atggtcctct cattggaaaa gttgatttga tgaagtttaa tcatcaccat gataccaaca
     4201 aatcaaatac caaggatttc attaaaaatt tgagtccgag tttgattgtt caaacttcgg
     4261 atagtctacc ttggaaaaat ggtgttgata gtgagtatgt taattggctc aaagaacgag
     4321 gaattgagag aatcaacgca gccagcaaag actatgatgc aacagttttt gatattcgac
     4381 aagacggttt agtaaacatt tcaacttcct acaagccgat tccaagtttt caagctggtt
     4441 ggcataagag ttcatatgga aactggtggt atcaagcgcc tgattctaca ggagaatatg
     4501 ctgtcggttg gaatgaaatt aagggtgaat ggtattactt taaccaaacg ggtatcttat
     4561 tacagaatca atggaaaaaa tggaacaatc gttggttcta tttgacagac tctggtgttg
     4621 ctgctaaaaa ttggaagaaa atcgctggaa tctggtatta ttttaacaaa gaaaaccaga
     4681 tggaaattgg ttggattcaa gataaagagc agtggtatta tttggatgtt gatggctcta
     4741 tgaagacagg ttggcttcaa tataaggggc aatggtatta ctttgctcca tcaggggaaa
     4801 tgaaaacggg ctgggtaaaa gataaagaag cctggtacta tatggattct actggtatca
     4861 tgaagacagg tgagatagaa gttgctggac atcattacta tctagaagaa tcaggagcta
     4921 tgaagcaagg ctggcttaaa aaggcaaatg attggtattt ctacaagaca gatggttcac
     4981 gagctatagg ttggattaaa gacaaggata aatggtactt cttgaaagaa aatggtcaat
     5041 tacttgtgaa tggtaagaca ccagaaggct atactgtgga ttcaagtggt gcctggttag
     5101 tggatgttcc gatcgagaaa tctgctacaa ttaaaactac aagtcattca gaaataaaag
     5161 aatccaaaga agtagtgaaa aaggatcttg aaaataaaga aacgagtcaa catgaaagtg
     5221 ttacaaattc ttcaactagt caagatttga catcctcaac ttcacaaagc tctgaaacga
     5281 gtgtaaacaa atcggaatca gaacagtagt agaaaagaag gttttagggc cttctttttc
     5341 ctatcaactc ttttctattt cctgttattc atgttataat ggataaatat gaataatcgg
     5401 agtgagacta tgaaatacaa acggattgtc tttaaggtgg gtacttcttc tctgacaaat
     5461 gaggatggaa gtttatcacg tagtaaggta aaggatatta cccagcagtt ggctatgctg
     5521 cacgaggctg gtcatgagtt gattttggtg tcttcaggtg ccattgcggc tggttttgga
     5581 gccttaggat ttaaaaagcg tccgactaaa attgctgata aacaggcttc agcagcggta
     5641 gggcagggac ttttgttgga agaatatacg accaatcttc tcttgcgtca aatcgtttct
     5701 gcacaaatct tgctgaccca agatgacttt gtggataagc gtcgttataa aaatgcccat
     5761 caggctttgt cggttttgct caaccgtggg gcaattccta tcatcaatga gaatgatagt
     5821 gtcgttattg atgagctcaa ggttggggac aatgacactc taagtgctca agtagcggcg
     5881 atggtccaag cagacctttt agttttcttg acagatgtgg acggtctcta tactggaaat
     5941 cctaattcag atccaagagc caaacgcttg gagagaatcg agaccatcaa tcgtgagatt
     6001 attgatatgg ctggtggagc aggttcgtca aacggaactg ggggtatgtt aaccaaaatc
     6061 aaggctgcaa ctatcgcgac ggaatcagga gttcctgttt atatctgctc atccttgaaa
     6121 tcagattcca tgattgaggc ggcagaggag accgaggatg gttcttactt tgttgctcaa
     6181 gagaaggggc ttcgtaccca gaaacaatgg cttgccttct atgctcagag tcaaggttct
     6241 atttgggttg ataaaggggc tgcggaagct ctctctcaac atggaaagag tcttctctta
     6301 tctggtatcg ttgaagcaga aggagccttt tcttacggtg atatcgtgac agtatttgac
     6361 aaggaaagtg gaaaatcact tggaaaagga cgcgtgcaat ttggagcatc tgctttggag
     6421 gatatgttgc gttctcaaaa agccaagggt gtcttgattt accgtgacga ctggatttcc
     6481 attactcctg aaatccaact actttttaca gaattttaga ggtaaactat ggtgagtaga
     6541 caagaacaat ttgaacaggt acaggctgtt aaaaaatcga ttaacacagc tagtgaagaa
     6601 gtgaaaaacc aagccttgct agccatggct gatcacttag tggctgctac tgaggaaatt
     6661 ttagcggcta atgccctcga tatggcagcg gctaagggga aaatctcaga tgtgatgttg
     6721 gatcgtcttt atttggatgc agatcgtata gaagcgatgg caagaggaat tcgtgaagtg
     6781 gttgccttac cagatccaat cggtgaagtt ttagaaacaa gtcagcttga aaatggtttg
     6841 gttatcacaa aaaaacgtgt agctatgggt gtcatcggta ttatctatga aagccgtcca
     6901 aatgtgacgt ctgatgcggc tgctttgact cttaagagtg gaaatgcggt tgttcttcgt
     6961 agtggtaagg atgcctatca aacaacccat gccattgtca cagccttgaa gaagggcttg
     7021 gagacgacta ctattcatcc aaatgtgatt caactggtgg aggatactag ccgtgaaagt
     7081 agttatgcta tgatgaaggc caagggctat ctagaccttc tcattcctcg tggaggagct
     7141 ggcttgatca atgcagtggt tgagaatgcg attgtacctg ttatcgagac agggactggg
     7201 attgtccatg tctatgtgga taaggatgca gacgaagaca aggcgctgtc tatcatcaac
     7261 aatgctaaaa ccagtcgtcc ttctgtttgt aatgccatgg aggttctgct ggttcatgaa
     7321 aacaaggcag caagcatcct tcctcgcttg gatcaaatgc tggttgcaga tcgaaaagaa
     7381 gctgggttgg aaccaattca attccgccta gatagcaaag caagccagtt tgtttcaggt
     7441 caagctgctc aagcacaaga ctttgatacc gagtttttag actatattct agctgttaag
     7501 gttgtgagca gtttagaaga agcggttgcg catattgaat cccacagtac ccatcattcg
     7561 gatgctattg tgacggaaaa tgctgaagct gcagcatact ttacagatca agtggactct
     7621 gcagcggtgt atgttaatgc ctcaactcgt ttcacagatg gaggacaatt tggtcttggt
     7681 tgtgaaatgg ggatttctac tcagaaattg cacgcgcgtg gtccaatggg cttgaaagag
     7741 ttgaccagct acaagtatgt ggttgctggt gatgggcaga taagggagta agagatgaag
     7801 attggattta tcggtttggg gaatatgggt gctagcttgg caaaatctgt cttgcagact
     7861 aggacgtcag atgagattct ccttgccaat cgtagtcaag ctaaggtaga tgctttcatt
     7921 gcagactttg gtggtcaggc ttccagcaat gaagaaatgt ttgcagaagc agatgtgatt
     7981 tttctaggag ttaagcctgc tcagttttct gaactgcttt ctcaatatca gaccatcctt
     8041 gaaaaaagag aaagtcttct tttgatttcg atggcagctg gattgacctt agaaaaacta
     8101 gcaagtctta tcccaagcca acaccgaatt attcgtatga tgcctaatac ccctgcttct
     8161 atcgggcaag gagtgattag ttatgccttg tctcctaatt gcagggctga ggacagtgag
     8221 atcttttgtc agcttttagc caaggctggt ctcttggttg aattaggaga aagcttaatc
     8281 aatgcagcga caggtcttgc aggttgtgga ccagcctttg tctatctctt tattgaggcc
     8341 ttggcagatg caggtgttca gacgggatta ccacgagaaa cagccttgaa aatggcagcc
     8401 caaactgtgg taggagctgg gcaattggtc ctagaaagcc agcaacatcc tggagttttg
     8461 aaagatcaag tttgtagtcc aggcggttcg actattgctg gcgtagcaag cctagaagcg
     8521 catgcttttc gaggcacagt catggaggca gttcatcaag cctataaacg aacacaagaa
     8581 ctaggtaaat aagaggtagc tttatctgcc tcttttatgg tggttgatac tcttcgaaaa
     8641 tcaaattcaa actgcgtcag cttcaccttg ccgtactcaa gtacagcctg cggctagctt
     8701 cctagtttgc tctttgattt tcattgagta tgaaatgaga agacaaaaag attgtcacaa
     8761 acccctattt ttttgataga atagaagtag taaaaaagaa atgagttaga catgtcaaaa
     8821 ggatttttag tctctcttga gggaccagag ggagcaggca agaccagtgt tttagaggct
     8881 ctgctaccaa ttttagagga aaaaggagta gaggtgttga cgacccgtga acctggcgga
     8941 gtcttgattg gggagaagat tcgggaagtg attttggatc caagtcatac tcagatggat
     9001 gctaaaacag agctacttct ctatattgcc agtcgcagac agcatttggt ggaaaaagtt
     9061 cttccagccc ttgaagctgg caagttggtc atcatggatc gttttatcga tagttctgtt
     9121 gcctatcagg gatttggtcg tggcttagat attgaagcca ttgactggct caatcagttt
     9181 gcgacagatg gcctcaaacc cgatttgaca ctctattttg acatcgaggt ggaagaaggg
     9241 ctggctcgta ttgctgctaa tagtgaccgc gaggttaatc gtttggattt ggaagggttg
     9301 gacttgcata aaaaagttcg tcaaggctac ctttctcttc tggataaaga gggaaatcgc
     9361 attgtcaaga ttgatgctag tctccctttg gagcaagttg tggaaactac caagtctgtc
     9421 ttgtttgacg gaatgggctt ggccaaatga aacaagatca actaaaggct tggcaaccag
     9481 ctcagtttga ccgttttgtc cgtatcttag aacaagacca gctcaatcac gcctatctct
     9541 tttcaggttt ctttggaagc ttggaaatag cgcaattttt agctaagagc ctcttttgta
     9601 cggataaagt tggcgtctta ccatgtgaga aatgccgaag ttgcaagctg attgaacagg
     9661 aagagtttcc agatgtcacc ttgattaagc cagtcaatca ggtcatcaag acagaacgca
     9721 ttcgggaatt ggtgggacag ttttctcaag cagggattga aagccagcaa caggtcttta
     9781 tcatcgagca agcggataaa atgcatccca acgcagccaa ttctctgctc aaggtcatcg
     9841 aagaacccca gagtgaagtt tatattttct tcttgactag cgatgaggaa aagatgttac
     9901 cgacaatccg aagtcggacc cagatcttcc actttaaaaa gcaagaagaa aaacttatct
     9961 tactcttaga acaaatggga cttgttaaga aaaaagcgac tcttttagct aagtttagtc
    10021 aatcgcgagc tgaagcagaa aagttggcta atcaggcaag tttttggacc ttggtcgatg
    10081 aaagtgaacg cctgctgact tggttagtag ctaagaaaaa agaaagttat ctacaggttg
    10141 ccaaattagc caacttggca gatgataagg aaaaacagga tcaggtttta cggattcttg
    10201 aagttctctg tgggcaggac ctcttgcagg taagagtaag agtgattcta caagatttac
    10261 tagaagctag aaaaatgtgg caagctaatg tcagctttca aaatgccatg gaatatctgg
    10321 tcttgaaaga aatataaact caaaaatgaa tgataaagaa aggaaagggc tgttttatgg
    10381 acaaaaaaga attatttgac gcgctggatg atttttccca acaattattg gtaaccttag
    10441 ccgatgtgga agccatcaag aaaaatctca agagcttggt agaagaaaat acagctcttc
    10501 gcttggaaaa tagtaagttg cgagaacgct tgggtgaggt ggaagcagac gctcccgtca
    10561 aggccaagca tgttcgcgaa agtgtccgtc gtatttaccg tgatggattt cacgtatgta
    10621 atgattttta tggacaacgt cgagagcagg acgaagaatg tatgttctgt gatgagttgt
    10681 tatacaggga gtaagcatgc aaattcaaaa aagttttaag gggcagtctc cctatggcaa
    10741 gctgtatcta gtggcaacgc cgattggcaa tctagatgat atgacttttc gtgctatcca
    10801 gaccttgaaa gaagtggact ggattgctgc tgaggatacg cgcaatacag ggcttttgct
    10861 caagcatttt gacatttcca ccaagcagat cagttttcat gagcacaatg ccaaggaaaa
    10921 aattcctgat ttgattggtt tcttgaaagc agggcaaagt attgctcagg tctctgatgc
    10981 cggtttgcct agcatttcag accctggtca tgatttggtt aaggcagcta ttgaggaaga
    11041 aattgcagtt gttacagttc caggtgcctc tgcaggaatt tccgccttga ttgccagtgg
    11101 tttagcgccg cagccacata tcttttacgg ctttttaccg agaaaatcag gccagcaaaa
    11161 gcaatttttc gactcgaaaa aagactatcc tgaaactcag attttttatg aatcgcccca
    11221 tcgtgtggca gatacattgg aaaatatgct agaagtctac ggtgaccgct cggtagtctt
    11281 ggtcagggaa ttgactaaaa tctatgaaga ataccaaaga ggtaccattt ctgaattgct
    11341 ggaaagcatc tctgaaacgt ctctcaaggg tgaatgcctt cttattgttg aaggtgccag
    11401 tcaggatgtg gaggaaaaag acgaggagga cttgttctta gaaatccaag cccgcatcca
    11461 acatggtatg aagaaaaatc aagccattaa ggaagttgcg aaatactacc agtggaataa
    11521 aagtcagctt tatgctgcct accacgaatg ggaagaaaat caagaaaatg actaaacagg
    11581 gaagtttgcc ttcttccctt tttttgttat actagtagaa gaaaaaaata gaaagatttg
    11641 tgggagtgaa aaagtcctgt ggactttttc agcctgagcc aagaaactcg aaagctcgta
    11701 agtctgattg gctttttcaa tgtgaatctt aacttcacac tcccaaagag gtattagtgt
    11761 cgtgtctcaa tcttatatca atgttatcgg tgctggtttg gcaggttctg aagcagctta
    11821 ccaaatcgca gagcgtggta ttccagttaa actttatgaa atgcgtggtg tcaagtcgac
    11881 tcctcagcat aaaacagaca attttgctga gttggtttgt tccaattctt tgcgtgggga
    11941 tgctttgaca aatgcagttg gtcttctcaa ggaagaaatg cgtcgcttgg gttctgttat
    12001 cttggaatct gctgaggcta cacgtgttcc tgccggtgga gcccttgcgg tggatcgtga
    12061 tggtttctct caaatggtga ccgaaaaagt ggtcaaccac cccttgattg aagtggtgcg
    12121 tgatgaaatt acagaattgc caacagatgt tattacagtt gtggcgactg gtcccttgac
    12181 tagcgatgcc ctggctgaaa agattcatgc ccttaataat ggcgatggct tctatttcta
    12241 cgatgcagca gcgcctatta tcgatgtcaa cactatcgat atgagtaagg tctatctcaa
    12301 atctcgttat gataagggag aagcagccta tctcaatgct ccaatgacca agcaagagtt
    12361 tatggatttc catgaagcct tggtcaatgc ggaagaagca ccgctcaatt cttttgaaaa
    12421 agaaaagtac tttgaaggat gtatgccaat cgaagttatg gccaaacgtg gcattaaaac
    12481 tatgctttat ggccctatga agccagtcgg tcttgagtat ccggacgact acacaggacc
    12541 tcgtgatgga gaatttaaaa caccttatgc ggttgtgcaa cttcgtcagg ataatgcagc
    12601 tggtagccta tacaatattg ttggtttcca gacccacctc aaatggggag aacaaaagcg
    12661 tgtcttccaa atgattccgg gtcttgaaaa tgcggagttt gtccgttatg gtgtcatgca
    12721 tcgcaattct tacatggatt caccaaatct tcttgagcag acttaccgtt ccaagaaaca
    12781 accaaatctc ttctttgctg gtcagatgac gggggtggaa ggctatgttg agtcggcggc
    12841 ttcgggctta gttgcgggaa ttaacgcagc tcgtctcttc aaggaagaaa gcgaggttat
    12901 tttccccgag acgacagcga ttggaagctt agctcattac attacccatg ccgacagcaa
    12961 acatttccaa ccaatgaatg tcaattttgg gatcatcaag gagttggaag gcgagcgtat
    13021 ccgtgataag aaagctcgtt atgaaaaaat tgcagagcgt gcccttgccg acttagagga
    13081 atttttgact gtctaatttt tttgaaagaa ttgttcatga tactataaaa atcttagaaa
    13141 ttgtgataaa ataggtagga tgaaagaagg agagtgaaaa tggcgaatcc caagtataaa
    13201 cgtattttaa tcaagttatc aggtgaagcc cttgccggtg aacgtggcgt agggattgat
    13261 atccaaacag ttcaaacaat cgcaaaagag attcaagaag ttcatagctt aggtatcgaa
    13321 attgcccttg ttattggtgg aggaaatctc tggcgtggag aacctgcagc agaagcaggt
    13381 atggaccgtg ttcaggcaga ttacactgga atgcttggga ctgttatgaa tgctcttgtg
    13441 atggcagatt cattgcaaca agttggggtt gatacgcgtg tacaaacagc tattgccatg
    13501 caacaagtgg cagagcctta tgtccgtgga cgtgcccttc gtcaccttga aaaaggccgt
    13561 atcgttatct ttggtgctgg aattggttca ccatacttct cgacagatac aacagcggcc
    13621 cttcgtgcag ctgaaatcga agcagatgcc atcctcatgg ctaaaaatgg cgtcgatggt
    13681 gtgtacaatg ccgatcctaa gaaggacaag acagccgtta agtttgaaga attgacccac
    13741 cgtgatgtta tcaacaaagg tcttcgtatc atggactcaa cagcctcaac cctctcaatg
    13801 gacaacgaca ttgacttggt tgtcttcaac atgaaccaat caggcaacat caaacgtgtc
    13861 gtatttggtg aaaatatcgg aacaacagtt tcaaataata tcgaagaaaa ggaataagaa
    13921 agaatatggc taacgtaatt attgaaaaag ctaaagagag aatgacccag tctcaccaat
    13981 cacttgctcg tgaatttggt ggtatccgtg ctggtcgtgc caatgcaagc ttgcttgacc
    14041 gtgtacatgt agaatactat ggagtcgaaa ctcctcttaa ccaaatcgct tcaattacga
    14101 ttccagaagc gcgtgttttg ttggtaacac catttgacaa gtcttcattg aaagacatcg
    14161 aacgtgcctt gaacgcttct gatcttggta tcacaccggc taatgacggt tctgtgattc
    14221 gcttggttat cccagctctt acagaagaaa ctcgtcgtga ccttgctaaa gaagtgaaga
    14281 aggtcggcga aaatgctaaa gtggctgtcc gcaatatccg tcgcgatgct atggacgaag
    14341 ctaagaaaca agaaaaagca aaagaaatca ctgaagacga attgaagact cttgaaaaag
    14401 atattcaaaa agtaacagac gatgctgtta aacacatcga cgacatgact gctaacaaag
    14461 agaaagaact tttggaagtc taaaaataaa cagaaaaact cagttggcat tgctggctga
    14521 gttttattcg aaagaaggaa atatgaatac aaatcttgca agttttatcg ttggactgat
    14581 catcgatgaa aatgaccgtt tttactttgt gcaaaaggat ggtcaaacct atgctcttgc
    14641 taaggaagag ggccaacata cagtagggga tacggtcaaa ggttttgcat acacggatat
    14701 gaagcaaaaa ctccgcctga caaccttaga agtgactgcc actcaggacc aatttggttg
    14761 gggacgtgtc acagaggttc gtaaggactt gggtgtcttt gtggatacag gccttcctga
    14821 caaggaaatc gttgtgtcac tcgatattct ccctgagctc aaggaactct ggcctaagaa
    14881 gggcgaccaa ctctacatcc gtcttgaagt ggataagaaa gaccgtatct ggggcctctt
    14941 ggcttatcaa gaagactttc aacgtctcgc tcgtcctgct tacaacaaca tgcagaacca
    15001 aaactggcca gccattgttt accgtctcaa gctgtcagga acttttgttt acctaccaga
    15061 aaataatatg cttggtttta ttcatcctag cgagcgttac gcagagccac gtttggggca
    15121 agtattagat gcgcgcgtta ttggtttccg tgaagtggac ctcactctga acctctccct
    15181 caaaccacgc tcctttgaaa tgttggaaaa cgatgctcag atgattttga cttatttgga
    15241 aagcaatggc ggtttcatga ccttaaatga taagtcatct ccagacgaca tcaaggcaac
    15301 ctttggcatt tctaaaggtc agttcaagaa agctttaggt ggtcttatga aggctggtaa
    15361 aatcaagcaa gaccagtttg ggacagagtt gatttaggga gatttatgag aaaatcattt
    15421 tacacttggc tcatgaccga gcgcaatcct aaaagtaaca gtcccaaagc tatcttggca
    15481 gacctcgctt ttgaagaggc agcttttcca aaacacacag atgattttga tgaggtcagt
    15541 cgctttttgg aggagcatgc cagtttctct tttaacctag gagactttga cgctatctgg
    15601 caagaatact tagaacacta gcatttattc actaggtttg ggctagtaat ttctccatcc
    15661 ctctgctata ataaaaagaa ataaaaggat tagagaggtt ctttatttga aggaacattc
    15721 aatagacatt caactgagtc atccagatga cttgtttcat ctttttggtt ccaatgaacg
    15781 ccatcttcgt ttgatggaag aagagctgga tgtggtgatt catgctcgta cggagattgt
    15841 ccaggttttg ggagaagaga ctgcctgtga ggaagcccgt caggttatcc aagctttgat
    15901 ggtcttggtg aaccgtggga tgaccgttgg tacgccagat gtggtgactg caattagcat
    15961 ggtcaaaaac gatgaaattg ataagtttgt cgccctttac gaagaagaaa ttatcaagga
    16021 taatactggg aaaccgatcc gcgttaaaac gttaggtcaa aaactttatg tggacagtgt
    16081 caaacagcat gatgtgacct ttggaattgg gcctgcaggg acggggaaga cctttcttgc
    16141 agttactttg gcagtgactg cccttaaacg tgggcaagtc aagcggatta tcttgacacg
    16201 tccagcggtg gaagcaggag agagtctagg ttttcttccg ggtgatctta aggagaaggt
    16261 agatccttac cttagacctg tttatgatgc cttgtatcag attctgggga aagaccaaac
    16321 aactcgactc atggagcgtg aaattatcga gattgcgccc cttgcctata tgcgtggacg
    16381 gaccttggat gatgcctttg tcattctcga tgaggcgcaa aatacgacca tcatgcagat
    16441 gaagatgttc ttgactcgtt taggttttca ttctaagatg attgtcaatg gagatattag
    16501 tcagattgac ctgccacgta atgtcaagtc cggtttgatt gatgctcaag agaaactcaa
    16561 gaacatccat cagattgact ttgttcattt ttcagccaag gatgtggttc gccatccagt
    16621 tgtcgctcag attatccgag cctatgaata ttctactgaa gttgcacacg actgattttg
    16681 aggaagttcg cctgcaaaag aatatacttt tgagagagga aaatccagtt gtataggcta
    16741 aaggttttat ccaaaggttt gagacaacga ttaggcacga tggaaagaac ttttatgtgg
    16801 ctgatgacga tcagtgcatc ttcctgtgtc ataatcacag ggcacaagaa agtaggaatt
    16861 tgaaaagatg attgaccaac tatctaagta ttacagttgt aggatactaa ctgaaaagga
    16921 tattccaagt attttatctt tatatgaaag taatcctctg tattttcagc attgtccacc
    16981 agagccaaat tttgcaactg taaaagagga catgctttgt ctacctgaag gtaaagctaa
    17041 ggctgataag ttttttgttg gattttggaa tggatctgac cttgtggctg ttatggattt
    17101 tgtctatgca tatcctgatg aggagactgt ttttattggt ttgtttatgg ttgatcaagc
    17161 ctatcagagg aaagggattg gtagtcatat tgtgacagaa gcactagctt attttgctaa
    17221 gaactgtcga aaggcacgtt tggcttatgt taagggaaat ccgcaatctc agcatttttg
    17281 ggaaaagcag ggctttaaat caattggatg cgaggttaag caagaactct atacggttgt
    17341 tatcgctgaa cagagcctag aagattagaa atggcatcaa gtaagaacta tttggaattt
    17401 gttttggaac aattatcagg attagatgat gtgacttacc gttccatgat gggggagtat
    17461 attctttact tccgcggcaa gattattggc ggcatttatg acgatcgctt tttagttaaa
    17521 cccgtgcaag cagtcttaga taagattgac caatcttctt ttgagtttcc atacaaaggt
    17581 gccaaagaaa tgatttgagt ggaagaactt gataataaga tgtttctata agacctgatt
    17641 ttagctatgt ataaccaact gccaacgccc aaacctaaaa agaaaaagca agggtgaacg
    17701 aagtaaaaaa gaagtctgct aaggccctgt ctttgcacgg gtaaaatttt atatataaaa
    17761 agaagctggg actaaagagc tcagcttcct ttggtttata taattgtcat tacaagacga
    17821 agtggttggg cgaaactctg ttgattttat tcaatttaga gtttcttatg cacaattgag
    17881 tctggaacga aagtctccag ttgcaaagta tacagtacaa taaaccaacg atgtaatagc
    17941 tgatgacaca aagcacagtg ggtaggactt gcgaagtcac ccttttcttt tcaaaattta
    18001 tactaaatca ttgatatcag tgtagtcacg attaagtcct tgagcaactg gtaggctagt
    18061 taagtaacct tgataagtgg tcacaccttg acgcaagcct tcatcttcag agattgcttg
    18121 tgcgaatcct ttgccagcca aagcttcgat ataaggaaga gtgacattgg ttagggcgat
    18181 ggttgaagtg cgagcaaccg caccagggat attggcaacg gcatagtgga gaacaccgtg
    18241 tttttcatag acgggttcat cgtgcgttgt cacacggtca gctgtttcga taacgccacc
    18301 ttggtcaaca gcaacgtcaa cgatacagag cctggacgca tttgtttgac catctcatct
    18361 gtcaccaatt ccggtgcttt tgcaccaggg atgagaatgg ctccaatcac cacatcagca
    18421 tctctcacac ttgcttcaat gttgaatgaa ttagatataa gagtttgaat ttgacttcca
    18481 aagacttctt ctagaactga gagacgcttg gcactaatat ctaaaatagt cacttgagca
    18541 ccaagaccaa gggcgatgcg ggcagcatgt gtaccgacga caccatcacc gatgatagtt
    18601 acttttcctt ttggaacacc tggtacacca ccaggtagaa caccagatcc accagcttgc
    18661 ttagtaagga agtgagctcc gatttaaaca gccatacgat ctgcaacctc actcatagga
    18721 acgaggagcg gtagttgtcc ttgattgtca cgaacagttt cataggcaat tcctgttgtt
    18781 tttgctgcta acatagcatc tgctaattct ggagcagcgg ccatgtgcaa gtaggtgaag
    18841 agaagaagat cgtcgcgcaa gtaaccgtat tcagaactta aaggttcttt tactttcaca
    18901 accaactctg ctgcccaagc ttcaccagca gtagcgacaa tctcagctcc ttgcttttga
    18961 tagtcagcat cagtaaagcc agaaccgaga ccagcatttg tttcgataag gacacgatga
    19021 ccacgactaa ctaagctatg aacacctgca ggtgtgaggg cgacacggtt ttcgttattt
    19081 ttaatttctt ttgggattcc gattaacatt gagataacct acctttcaat tgacggtctt
    19141 gttttggttg tcacattcca gttcataaat caaaaatgtg acggtttcat tgtatatgaa
    19201 accgcttcaa aaatcaagaa aaacttgtca tccaaatttt ttatgctaga ctagtgaaaa
    19261 tcaagctttg attggttgga aaagtatgga atcaattttt gtaaaacttg cccagtatcc
    19321 gtctatagaa acggagcgtt tattgcttag acctgtaact ttggatgatg cggaagcaat
    19381 gtttgactat gcctcggaca agggtaatac acgttacact tttccaacca atcaaagctt
    19441 ggaagaaacc aagaataaca ttgctcagtt ctatttggct aatcccttgg gacgttgggg
    19501 aatagaacta aaaagcaatg gtcagtttat tggaaccatt gacttgcaca agattgattc
    19561 tgttcttaag aaggcagcta ttggctacat tatcaataaa aagtattgga atcaaggatt
    19621 aacgacagaa gccaatcgtg ctgtgattga gctagctttt gagaagatag ggatgaataa
    19681 gttgactgcc cttcacgata aggacaatcc tgcgtcagga aaggtcatgg agaaatcagg
    19741 catgcgtttt tcccatgcag aaccatatgc ttgtatggac cagcatgaaa aaggccgaat
    19801 cgtgacaaga gttcattatg tcttgaccaa ggaagactat tttgcaaata aataagcagt
    19861 tgaaaagaaa tttttcgact gttttttctt cctcttacga ataatctaag agaggagaaa
    19921 atatggaagc aattatcgag aaaatcaaag agtataaaat catcgtcatc tgtactggtc
    19981 tgggcttgct tgtaggagga tttttcctgc taaaaccagc tccacaaaca cctgtcaaag
    20041 agacgaattt gcaggctgaa gtcgcagctg tttccaagga tttggtatcc gaagaggaag
    20101 tgaacaagga agaaaaggaa gaaccccttg aacaagatct aatcacagta gatgtcaaag
    20161 gtgctgtcaa atcgccaggg atttatgact tgcctgtagg tagtcgagtc aatgatgctg
    20221 ttcagaaggc tggtggcttg acagagcaag cagacagcaa gtcgctcaat ctagctcaga
    20281 aagttagtga tgaggctctg gtttacgttc ctactaaggg agaagaagca gttagccaac
    20341 agactggttt ggggacagct tcttcaataa gcaaggaaaa gaaggtcaat ctcaacaagg
    20401 ccagtctgga agaactcaag caggtcaagg gactgggagg aaaacgagct caggacatta
    20461 tcgaccatcg tgaggcaaat ggcaagttca agtcagtaga cgagctcaag aaggtctctg
    20521 gcattggtgg caaaacaata gaaaagctta aagactatgt tacagtggat taagaatttc
    20581 tctattcccc taatttacct gagttttcta ttactttggc tttattacgc tattttctca
    20641 gcatcttatc ttgctttgtt gggctttgtt tttctgctag tctgtctctt tatccaattt
    20701 ccgtggaaat ctgctggtaa agttctaata atttgcggaa tctttggatt ttggtttgtt
    20761 tttcaaaatt ggcaacagag tcaagcgagt caaaatctgg cggattctgt tgaaagggta
    20821 cggattttac ctgatactat taaggttaat ggtgatagtc tatcctttcg tggcaagtct
    20881 aacggtcgtg ctttccaagt ctattataaa ctccagtccg aggaggagaa agaagccttt
    20941 caagctttaa ccgacctgca tgagatagga ctagaaggga agctttcgga gccagaaggg
    21001 cagagaaatt ttggtggctt taattaccaa gcctatctga agactcaggg aatttaccag
    21061 actctcaata tcaaaaaaat ccagtcactt caaaagattg gcagttggga tataggagaa
    21121 aacttgtcca gtttacgtcg aaaggctgtg gtttggatta agacgcactt tccagaccct
    21181 atgcgcaatt acatgacagg actcttgctg ggatatctga acaccgactt tgaggagatg
    21241 aatgagcttt attccagtct aggaattatc cacctctttg ccctatctgg catgcaggta
    21301 ggttttttca tgaatggatt taagaaactt ctcttgcgat tgggcttgac ccaagaaaag
    21361 ttgaaatggc tgacttatcc cttttccctt atctatgcgg gactaattgg attttcagca
    21421 tcggttattc gcagtctctt gcaaaagcta ctggctcaac atggggttaa gggcttggat
    21481 aattttgcct tgacggtgct tgtcctcttt attgtcatgc caaacttttt cttgacagca
    21541 ggaggagtct tgtcctgcgc ttatgctttt atcctgacca tgaccagcaa agaaggggag
    21601 gggctcaagg ctgttgctag tgaaagtcta gtcatctcct tgggcatatt gcccattcta
    21661 tccttctatt ttgcggaatt tcaaccttgg tctatccttt tgacctttgt cttttccttt
    21721 ctatttgact tgaccttctt accgctcttg tctattttat ttgtcctttc ctttctctat
    21781 ccagtcattc agctgaactt tatctttgaa tggttagagg gcattattcg cttggtctcg
    21841 caggtggcaa ggagaccgct tgtctttggt caacccaacg catggctttt aatcttattg
    21901 ttaatttcct tggctttggt ctatgatttg aggaaaaaca ttaaaggatt aacagtattg
    21961 agtttattga ttacaggtct ctttttcctt accaagtatc cactggaaaa tgaaatcacc
    22021 atgctggatg tggggcaagg agaaagtatt ttcctacggg atgtaactgg gaaaaccatt
    22081 ctcatagatg taggtggtaa ggcagaatct tataagaaaa tcgaaaaatg gcaagaaaag
    22141 atgacgacca gcaatgcaca gagaagcttg ataccctatc ttaaaagtcg aggagtagct
    22201 aagattgacc agctgatttt gacaaatacg gacaaggaac atgtcggaga tttgttagag
    22261 gtgaccaagg ctttccatgt aggggagatt ttagtatcaa aaggcagttt gaagcagaag
    22321 caatttgtgg tagaactaca ggcgactcaa acaaaggtgc gtagtatgac agtaggggag
    22381 aacttgccca tttttggaag tcagttagaa gttctatccc caaggaaaat gggagatgga
    22441 ggacacgatg ataccctagt tctgtatggg aaattcttgg ataagcaatt tctcttcacg
    22501 ggaaatttgg aggagaaagg agagaaggac ttgctgaagc actatccaga cttgaaagta
    22561 aatgttttga aagctagcca acatggcaat aaaaaatcat caagtccagc ctttctagaa
    22621 aaactcaaac cagagcttac tcttatctca gttggaaaga gcaatcgaat gaaactcccc
    22681 catcaggaaa cattgacacg actggaaggt atcaatagca aagtttaccg aactgaccag
    22741 caaggagcta tacggtttaa aggttggaat agttggaaaa tcgaaagtgt tcgataggaa
    22801 agataaatgt tgtagattag tgaaataaac taaaaatttg ttgcataata atgataaaaa
    22861 tggtataatg aaaacgtatt caatactgag gatataaaat cattaaaaat tagcaaaaat
    22921 cgtttaatta gttagtttat gattattggt ctttttcagt ccagtatatc tgctgtgaca
    22981 gccactaaaa gttacaagta tgattggaat acggttttgg aatatagtat caactatcac
    23041 gaccatcagt atgcttggat tccggaatgg tctcgttatt acagctattc tgagtataaa
    23101 gttggcggag gctggaacta cgctcgttat gaggtcataa actattacag cggaggctat
    23161 taattcttaa agagtgagaa aaaggagggc tagatatgtt gcagcttact catgtgacct
    23221 taaaaacgcg acaagtcatc ttgcaagatg cggatttcac ctttaaaaag ggtaggtttt
    23281 atggtcttct tgctatcaat ggctctggaa agacgaccct gttccgtgcc attagcaatt
    23341 taattcccat aagtagtgga aatatcgcag ttcctccttc tttgttttat tatgagagcg
    23401 ttgaatggct ggatggaaac ttaagtggga tggactacct tcgtctcata aaaaacatct
    23461 ggaagtcaga cctaaacttg agagatgaaa tcgcctactg ggaaatggct gactatatca
    23521 gtcttcccat ccgcaagtat tccttaggca tgaagcaacg cttggtgatt gctatgtatt
    23581 ttctcagtca ggccaaatgc tggctcatgg atgagattac aaatggctta gacgagtatt
    23641 atcgacagaa gttttttgat aggctagcac aaatcgatag acaagaacag ctggttcttt
    23701 taagttccca ctataaggaa gagttggttg atatctgcga tagagtagta accattcatc
    23761 aggggcagat agaagaggtt tagtttatga aagatgttag tctattttta ttgaaaaaag
    23821 ttttcaaaag tcgtttaaac tggattatct tacttttatt tgcatctgta ctcggtgtta
    23881 ccttttattt aaatagtcag actgcaaact cacacagctt ggagagcagg ttggaaagtc
    23941 gcattgcagc caacgagagg gctatcaatg aaaatgaaga gaaactctcc caaatgtctg
    24001 ataccagctc ggaggaatac cagtttgcta aaaataattt agacgtgcaa aaaaatcttt
    24061 tgacgcgaaa gacagaaatt ctgactttat taaaagaagg gcgctggaaa gaggcctact
    24121 atctgcagtg gcaagatgaa gagaagaatt atgaatttgt atcaaatgac ccgactgcta
    24181 gctctggctt aaaaatgggg gttgaccgcg aacggaagat ttaccaagcc ctgtatccct
    24241 tgaacataaa agcacatact ttggagtttc cgacccacgg gattgatcag attgtctgga
    24301 ttttagaggt tatcatccca agcttgtttg tggttgcaat tatttttatg ctaacacaac
    24361 tatttgcaga aagatatcaa aatcatctgg acacagctca cttatatcct gtttcaaaag
    24421 tgacatttgc aatatcctct cttggagttg gagtgggata tgtaactgtg ctgtttatcg
    24481 gaatctgtgg cttttctttt ctagtgggaa gtctgataag tggttttgga cagttagatt
    24541 atccctaccc aatttatagc ttagtgaatc aagaagtaac tattgggaaa atacaagatg
    24601 tattatttcc tggcttgctc ttagctttct tagcctttat cgtcattgtg gaagttgtgt
    24661 acttgattgc ttactttttc aagcaaaaaa tgcctgtcct ctttctttca ctcattggga
    24721 ttgttggctt attgtttggc atccaaacaa ttcagcctct tcaaaggatt gcacatctga
    24781 ttccctttac ttacttgcgt tcagtggaga ttttatctgg aagattatct aagcagattg
    24841 ataatgtcga tctaaattgg agcatgggga tggtcttact tccttgcctg attatccttt
    24901 tgttagtggg gattctattt atcgaaagat ggggaagttc acgtaaaaag gaagttttta
    24961 aagtttagtt ttcctatagg gaagctaaag aaaaactaac atagggagag aatcaacttg
    25021 gttctctctt tttgattcga aaaccaaacc aaaatataaa cacaaacttt tcaaaaaata
    25081 actttttatc ttgacaagag ctagaaaact tggtatcata taaaagttga gaaaagcaga
    25141 agtgagagct tctcgccttg tgacattaag ttgcctggcc ctacggatga aaagtttcga
    25201 agaaacgcta tcataacgtg cgggcttgta tatttacgag tccgctattg tttttctcta
    25261 ataaaacaaa agaggtgaaa accatagcaa agcaagactt attcatcaat gatgagattc
    25321 gtgtacgtga agttcgcttg attggtcttg agggagaaca gctaggcatc aagccactca
    25381 gtgaagcgca agctttggct gataacgcta atgttgacct agtattgatt caaccccaag
    25441 ccaaaccgcc tgttgcaaaa attatggact acggtaagtt caaatttgag taccagaaga
    25501 agcaaaaaga acaacgtaaa aaacaaagcg ttgttactgt gaaagaagtt cgtctaagtc
    25561 caactattga caagggtgac tttgatacaa aacttcgtaa tgcacgtaag ttccttgaaa
    25621 aaggaaataa agttaaggta tctattcgct ttaagggtcg tatgattacc cataaagaga
    25681 ttggtgcaaa agttttagcc gagtttgctg aagcaactca agatattgcc atcatcgaac
    25741 aacgtgctaa aatggatgga cgtcaaatgt tcatgcaatt ggcgccagcg actgacaaaa
    25801 aataattgtc agaaagttaa ataaaggaga aaaaatcatg ccaaaacaaa aaacacaccg
    25861 cgcatcagct aaacgtttca aacgtacagg ttctggtgga cttaaacgtt tccgtgctta
    25921 cacttctcac cgtttccacg gaaaaactaa gaaacaacgt cgtcatcttc gtaaagcatc
    25981 tatggtgcat tcaggagatt acaaacgtat caaagcaatg cttactcgct tgaaataata
    26041 gcttgactgt aagtaaacaa ttctagaaaa tatttggagg aaataaaaca tggcacgtgt
    26101 taaaggtggc gttgtatcac gcaaacgtcg taaacgtatt cttaaattag caaaaggtta
    26161 ctatggagct aaacacatct tgttccgtac tgcaaaagaa caagtaatga actcttacta
    26221 ctatgcatac cgtgaccgtc gtcagaaaaa acgtgacttc cgcaaattgt ggattactcg
    26281 tatcaatgcg gcggctcgta tgaacggact ttcatactca caattgatgc atggtttgaa
    26341 attggctgag atcgaagtta accgtaaaat gcttgctgac ttggctgtta acgatgcagt
    26401 agctttcaca gctcttgcag atgcagctaa agcaaaactt ggtaaataac aactgatgag
    26461 actggaacag gattgtccag tctttttaaa aataaaggag aaagatatgg cttcaaaaat
    26521 gctacacact tgcttacgag tagaaaatct tgaaaaatca attgcatttt atcaagatgc
    26581 ttttggtttt aaagaattgc gtcgcagaga ttttccagac catgccttca cgattgtcta
    26641 tctaggtctt gagggtgatg attatgagtt ggagttgact tataactacg atcacggtcc
    26701 gtatgtggtt ggtgatgggt ttgcccatat cgccctcagt acacctgatc ttgaggcact
    26761 tcatcaagaa cacagtgcaa aaggctatga agtgactgag ccaaatggtc taccaggaac
    26821 tacaccaaac tattactttg ttaaagaccc tgatggatat aaggtcgaag tcattcgtga
    26881 aaaataattt cgtgcaagtt aagtagaata ttcgttttct acttgacttt ttttagctga
    26941 aagagtatac taataaaaat tcaaccttta aggggagtcc agagagactc acaaggtgtc
    27001 agataaaaga atagtgcaat tttctagagg agaccttttg agtgtgctct cttgtgttgt
    27061 acgattttaa ctgaggcctt gcactagcaa ggtcttttct ttatctggtc cccttaaaat
    27121 ttaaggagga aaagttatga atctcacatg taagaagcgt ttgggtgtca ttcggttgga
    27181 aaccatgaag gtggttgcac aagaggaaat cgcgccagca atctttgaat tagtcctaga
    27241 aggagaaatg gttgaagcca tgcgagcagg ccaatttctt catctgcgtg taccggacga
    27301 tgcccatctc ttacgtcgtc ctatttcaat ttcgtctatt gacaaggcaa acaagcagtg
    27361 tcacctcatt tatcggattg agggagctgg gactgcaatt ttttcaacct taagtcaggg
    27421 agacactctt gatgtgatgg ggcctcaggg aaatggtttt gacttgtctg accttgatga
    27481 gcagaatcag gttctccttg ttggtggtgg gattggtgtt ccacccttgc ttgaggtggc
    27541 caaggaattg catgaacgtg gagtgaaagt agtgacagtc ctcggttttg ctaataagga
    27601 tgctgttatt ttgaaaacgg aattggctca gtatggtcag gtctttgtaa cgacagatga
    27661 tggttcttat ggcatcaagg gaaatgtttc cgttgttatc aatgatttag atagtcagtt
    27721 tgatgctgtt tactcgtgtg gggctccagg aatgatgaag tatatcaatc aaacctttga
    27781 tgatcaccca agagcctatt tatctctgga atctcgtatg gcttgtggga tgggagcttg
    27841 ctatgcctgt gttctaaaag taccagaaag cgagacggtc agccaacgcg tctgtgaaga
    27901 tggtcctgtt ttccgcacag gaacagttgt attataagga gaaaattatg actacaaatc
    27961 gattacaagt gtctctacct ggtttggatt tgaaaaatcc gattattcca gcatcaggct
    28021 gttttggctt tggacaagag tatgccaagt actatgattt agacctttta ggttctatta
    28081 tgatcaaggc gacaaccctt gaaccacgtt ttgggaatcc aactccaaga gtggcagaga
    28141 cgcctgctgg tatgctcaat gcaattggct tgcaaaatcc tggtttagag gttgttttgg
    28201 ctgaaaagct accttggctg gaaagagaat atccaaatct tcctattatt gccaatgtag
    28261 ctggtttttc aaaacaagag tatgcagctg tttctcatgg gatttccaag gcaactaatg
    28321 taaaagctat cgagctcaat atttcttgtc ccaatgttga ccactgtaat catggacttt
    28381 tgattggtca agatccagat ttggcttatg atgtggtgaa agcagctgtg gaagcctcag
    28441 aagtgccagt ttatgtcaaa ttaaccccga gtgtgaccga tatcgttact gtcgcaaaag
    28501 ctgcagaaga tgcgggagca agtggcttga ccatgatcaa tactctggtt ggaatgcgct
    28561 ttgacctcaa aaccagaaaa ccaatcttgg ccaatggaac aggtggaatg tcaggtccag
    28621 cagtctttcc agtagccctc aaactcatcc gccaagtagc ccaaacaaca gacctgccta
    28681 tcattggaat ggggggagtg gattcgactg aagctgccct agaaatgtat ctagctgggg
    28741 catctgctat cggagttgga acagctaact ttaccaatcc ttatgcctgc cctgacatca
    28801 tcgaaaattt accaaaagtc atggataaat acggtattag cagtctggaa gaactccgtc
    28861 aggaagtaaa agagtctctg aggtaaactg caatcaatct gttcttgatt ttttattagt
    28921 ttgtaatatg aatttaggag aattttggta caataaaata aataagaaca gaggaagaag
    28981 gttaatgaag aaagtaagat ttattttttt agctctgcta tttttcttag ctagtccaga
    29041 gggtgcaatg gctagtgatg gtacttggca aggaaaacag tatctgaaag aagatggcag
    29101 tcaagcagcg aatgagtggg tttttgatac tcattatcaa tcttggttct atataaaagc
    29161 agatgctaac tatgctgaaa atgaatggct aaagcaaggt gacgactatt tttacctcaa
    29221 atctggtggc tatatggcca aatcagaatg ggtagaagac aagggagcct tttattatct
    29281 tgaccaagat ggaaagatga aaagaaatgc ttgggtagga acttcctatg ttggtgcaac
    29341 aggtgccaaa gtaatagaag actgggtcta tgattctcaa tacgatgctt ggttttatat
    29401 caaagcagat ggacagcacg cagagaaaga atggctccaa attaaaggga aggactatta
    29461 tttcaaatcc ggtggttatc tactgacaag tcagtggatt aatcaagctt atgtgaatgc
    29521 tagtggtgcc aaagtacagc aaggttggct ttttgacaaa caataccaat cttggtttta
    29581 catcaaagaa aatggaaact atgctgataa agaatggatt ttcgagaatg gtcactatta
    29641 ttatctaaaa tccggtggct acatggcagc caatgaatgg atttgggata aggaatcttg
    29701 gttttatctc aaatttgatg ggaaaatagc tgaaaaagaa tgggtctacg attctcatag
    29761 tcaagcttgg tactacttca aatccggtgg ctacatggca gccaatgaat ggatttggga
    29821 taaggaatct tggttttacc tcaagtttga tggcaaaatg gctgaaaaag aatgggttta
    29881 cgattctcat agtcaagcct ggtactactt caaatccggt ggttacatga cagccaatga
    29941 atggatttgg gataaggaat cttggtttta cctcaaatct gatgggaaaa tagctgaaaa
    30001 agaatgggtc tacaattctc atagtcaagc ttggtactac ttcaaatccg gtggttacat
    30061 gacagccaat gaatggattt gggataagga atcttggttt tacctcaagt ctgatggcaa
    30121 aatggctgaa aaagaatggg tctacgattc tcatagtcaa gcctggtact acttcaaatc
    30181 tggtggctac atggcgaaaa atgagacagt agatggttat cagcttggaa gcgatggtaa
    30241 atggcttgga ggaaaagcta caaatgaaaa tgctgcttac tatcaagtag tgcctgttac
    30301 agccaatgtt tatgattcag atggtgaaaa gctttcctat atatcgcaag gtagtgtcgt
    30361 atggctagat aaggatagaa aaagtgatga caagcgcttg gctattacta tttctggttt
    30421 gtcaggctat atgaaaacag aagatttaca agcgctagat gctagtaagg actttatccc
    30481 ttattatgag agtgatggcc accgttttta tcactatgtg gctcagaatg ctagtatccc
    30541 agtagctttt catctttctg atatggaagt aggcaagaaa tattattcgg cagatggcct
    30601 gcattttgat ggttttaagc ttgagaatcc cttccttttc aaagatttaa cagaggctac
    30661 aaactacagt gctgaagaat tggataaggt atttagtttg ctaaacatta acaatagcct
    30721 tttggagaac aagggcgcta cttttaagga agccgaagaa cattaccata tcaatgctct
    30781 ttatctcctt gcccatagtg ccctagaaag taactgggga agaagtaaaa ttgccaaaga
    30841 taagaataat ttctttggca ttacagccta tgatacgacc ccttaccttt ctgctaagac
    30901 atttgatgat gtggataagg gaattttagg tgcaaccaag tggattaagg aaaattatat
    30961 cgatagggga agaacttttc ttggaaacaa ggcttctggt atgaatgtgg aatatgcttc
    31021 agacccttat tggggcgaaa aaattgctag tgtgatgatg aaaatcaatg agaagctagg
    31081 tggcaaggat tagtactata agtgaatatg atttgagtga atagtaagtt aaaaatcctg
    31141 atttcaagta aaatcaggat tttttcatgg atgcaatttt tttggagtct ggtgtgacgc
    31201 ggagggtctt ttgtcctgtg taagtgacaa agccgggttt tccaccagtt ggtttattga
    31261 gttttttgac ttcaatcata tctacctgca ccagattcga caggcgccct tgagagaagt
    31321 aggcagctaa ctctgctgcg tctgtcttga ctgcatcaga tgggtcaaga tttcctgaga
    31381 tgacaacatg gcttccagga atgtccttag catggaacca aagttcctcc ttgcgggcca
    31441 ttttaaaggt caattcctca ttttgaagat tgtttcgtcc gacatagatg atggttttgc
    31501 catcgcttgc tagatattgt tctagttttt tgcgtttctg gattttctcc cgttgtcttc
    31561 tgcggataaa acctgtttga atcaattctt cacggatttc agcgatttct tccagtccag
    31621 cttggttgag gacggtttct acactttcca gatagagaat agtggctttg gtttcttcaa
    31681 tcaaatcagt caagtatttg acagcttctt tgagtttctg ataccgttta aaatagcgtt
    31741 gggcattctg gttgggagtc agagccttat caagcgcaat catgataggt tggttggtat
    31801 agtagttgtc taggataacc tggtcttggt cgttaggcac ttggtgtagg aaggttgtca
    31861 gcaattctcc tttttgacga aattcttcag cgttgtctgt cgccagtaac tctttttcct
    31921 gttttttgag tttgtgtcgg tttttctgaa gttcattttc aacacgacga atcagttcac
    31981 tggcctgctg tttgacgcgg tcgcgctcag ccttatcctt atagtaggtg tccaacaaat
    32041 cagaaagatt tgcaaaaggc tctcccacct gatttgcaaa aggaactgga ctgaaggaag
    32101 tctcagtcaa gcatggcttg gtttcttgat tgaaaaaatt tcggaaagcg gaaagttttt
    32161 cactaaccag tatcctttcc aattcatttg ccgtatcgcg tcccagacct tgaaagaggc
    32221 tttgaagatt ttttgctgtt agttcttggg tttgcaggat ttcaaagagc ttttcatcct
    32281 tgatagtaaa aggattgaga gattctgtac ttggcggagc gatataggtc gatcctggaa
    32341 gtaaggtgcg gtagctattt tgtgaaaaac cgacgtgttt gataacttcg aggattttat
    32401 gactgctttt atcgaccagt agaatattac tgtgtttccc cataatttca ataatcaagg
    32461 tagcctggat atggtctcca atctcgtttt tattggaaac tgtcatttcc acaatacggt
    32521 cattttccac ttgctcaatc gactcaatca gggccccctg caaatacttt ctcaaaacca
    32581 tgataaaggt agaaggttga gctggatttt caaaagtcgt ttgggtcagc tgaatgcgtc
    32641 caaaaactgg atgggcagaa aggagcaggc gatggctttg gcgattgctg cggatttgca
    32701 agaccaactc ttgttcaaaa ggctgattga ttttctggat gcgaccattc actaattcgt
    32761 ttcgcaattc ctcaactatg tggtgtaaaa aaaatccatc aaatgacatc gttctctcct
    32821 tgtgattgta ttccatagta ttatatcaaa aaggtagaat aaaatcatgg aaatgtggta
    32881 taataaagcc aagtaaagag aaatgagaag cacatgtata ttgaaatggt agatgaaact
    32941 ggtcaagttt caaaagaaat gttgcaacaa acccaagaaa ttttggaatt tgcagcccaa
    33001 aaattaggaa aagaagacaa ggagatggca gtcacttttg tgaccaatga gcgtagtcat
    33061 gaacttaatc tggagtaccg tgacaccgac cgtccgacag atgtcatcag ccttgagtat
    33121 aaaccagaat tggaaattgc ctttgacgaa gaggatttgc ttgaaaatcc agaattggca
    33181 gagatgatgt ctgagtttga tgcctatatt ggggaattgt tcatctctat cgataaggct
    33241 catgagcagg ccgaagaata tggtcacagc tttgagcgtg agatgggctt cttggcagta
    33301 cacggctttt tacatattaa cggctatgat cactacactc cggaagaaga agcggagatg
    33361 ttcggtttac aagaagaaat tttgacagcc tatggactca caagacaata aacgaaaatg
    33421 gaaaaatcgt gacctgatat ccagtttaga atttgccatc acagggattt ttactgctat
    33481 caaggaagaa cgaaatatgc gaaaacatgc agtgacggct ctagtggtca tccttgcagg
    33541 ttttgttttt caggtgtcac gaatcgaatg gctctttctc ctattgagca ttttcttggt
    33601 agtagccttt gagattatca actctgctat tgaaaatatg gtggatttgg ccagtcacta
    33661 tcacttttcc atgctggcta aaaatgccaa ggatatggcg gctggcgcgg tattagtggt
    33721 ttctcttttc gcagccttaa caggtgcatt gatttttctc ccacgaatct gggatttatt
    33781 attttaaaca gtaagaggaa attatgactt ttaaatcagg ctttgtagcc attttaggac
    33841 gtcccaatgt tgggaagtca acctttttaa atcacgttat ggggcaaaag attgccatca
    33901 tgagtgacaa ggcgcagaca acgcgcaata aaatcatggg aatttacacg actgataagg
    33961 agcaaattgt ctttatcgac acaccaggga ttcacaaacc taaaacagct ctcggagatt
    34021 tcatggttga gtctgcctac agtacccttc gcgaagtgga cactgttctt ttcatggtgc
    34081 ctgctgatga agcgcgtggt aagggggacg atatgattat tgagcgtctc aaggctgcca
    34141 aggttcctgt gattttggtg gtgaataaaa tcgataaggt tcatccagac cagctcttgt
    34201 ctcagattga tgacttccgt aatcaaatgg actttaagga aattgttcca atctcagccc
    34261 ttcagggaaa taacgtgtct cgtctagtgg atattttgag tgaaaatctg gatgaaggtt
    34321 tccaatattt cccgtctgat caaatcacag accatccaga acgtttcttg gtttcagaaa
    34381 tggttcgcga gaaagtcttg catctaactc gtgaagagat tccgcattct gtagcagtag
    34441 ttgttgactc tatgaaacga gacgaagaga cagacaaggt tcacatccgt gcaaccatca
    34501 tggtcgagcg cgatagccaa aaagggatta tcatcggtaa aggtggcgct atgcttaaga
    34561 aaatcggtag catggcccgt cgtgatatcg aactcatgct aggagacaag gtcttcctag
    34621 aaacctgggt caaggtcaag aaaaactggc gcgataaaaa gctagatttg gctgactttg
    34681 gctataatga aagagaatac taagtagagg taggctcatg cctgcttctt gtttttacag
    34741 aaggaggact tatgcctgaa ttacctgagg ttgaaaccgt ttgtcgtggc ttagaaaaat
    34801 tgattatagg aaagaagatt tcgagtatag aaattcgcta ccccaagatg attaagacgg
    34861 atttggaaga gtttcaaagg gaattgccta gtcagattat cgagtcaatg ggacgtcgtg
    34921 gaaaatattt gcttttttat ctgacagaca aggtcttgat ttcccatttg cggatggagg
    34981 gcaagtattt ttactatcca gaccaaggac ctgaacgcaa gcatgcccat gttttctttc
    35041 attttgaaga tggtggcacg cttgtttatg aggatgttcg caagtttgga accatggaac
    35101 tcttggtgcc tgacctttta gacgcctact ttatttctaa aaaattaggt cctgaaccaa
    35161 gcgaacaaga ctttgattta caggtctttc aatctgccct tgccaagtcc aaaaagccta
    35221 tcaaatccca tctcctagac cagaccttgg tagctggact tggcaatatc tatgtggatg
    35281 aggttctctg gcgagctcag gttcatccag ctagaccttc ccagactttg acagcagaag
    35341 aagcgactgc cattcatgac cagaccattg ctgttttggg ccaggctgtt gaaaaaggtg
    35401 gctccaccat tcggacttat accaatgcct ttggggaaga tggaagcatg caggactttc
    35461 atcaggtcta tgataagact ggtcaagaat gtgtacgctg tggtaccatc attgagaaaa
    35521 ttcaactagg cggacgaggc acccactttt gtccaaactg tcaaaggagg gactgatgag
    35581 aaaaatcatc ggaatcactg ggggaattgc ctctggtaag tcaactgtga caaattttct
    35641 aagacagcaa ggctttcaag tagtggatgc cgacgcagtc gtccaccaac tacagaaatc
    35701 tggtggtcgt ctgtttgagg ctctagtaca gcactttggg caagaaatca ttcttgaaaa
    35761 cggagaactc aatcgccctc tcctagctag tctcatcttt tcaaatcctg aagagcaaaa
    35821 atggtctaag caaattcaag gggagattat ccgtgaggaa ctggctactt tgagagaaca
    35881 gttggctcag acagaagaga ttttcttcat ggatattccc ctactttttg agcaggacta
    35941 cagcgattgg tttgctgaga cttggttggt ctatgtggac cgagataccc aagtagaacg
    36001 cttaatgaaa agggaccagt tgtccaaaga tgaagctgag tctcgtctgg cagcccagtg
    36061 gcctttagaa aaaaagaaag atttggccag ccaggttctt gataataatg gcaatcagaa
    36121 ccagcttctt aatcaagtgc atatccttct tgagggaggt aggcaagatg acagagatta
    36181 actggaagga taatctgcgc attgcctggt ttggtaattt tctgacagga gccagtattt
    36241 ctttggttgt accttttatg cccatcttcg tggaaaatct aggtgtaggg agtgagcaag
    36301 tcgcttttta tgcaggctta gcaatttctg tctctgctat ttccgcggcg ctcttttctc
    36361 ctatttgggg tattcttgct gacaaatacg gccgaaaacc catgatgatt cgggcaggtc
    36421 ttgctatgac tatcactatg ggaggcttgg cctttgtccc aaatatctat tggttaatct
    36481 ttcttcgttt actaaacggt gtatttgcag gttttgttcc taatgcaacg gcactgatag
    36541 ccagtcaggt tccaaaggag aaatcaggct ctgccttagg tactttgtct acaggcgtag
    36601 ttgcaggtac tctaactggt ccctttattg gtggctttat tgcagaatta tttggcattc
    36661 gtacagtttt cttactggtt ggtagttttc tatttttagc tgctattttg actatttgct
    36721 ttatcaagga agtttttcaa ccagtagcca aggaaaaggc cattccaaca aaggaattat
    36781 ttacctcggt taaatatccc tatcttttgc tcaatctctt tttaaccagt tttgtcatcc
    36841 aattttcagc tcaatcgatc ggccctattt tggctcttta tgtacgcgac ttagggcaga
    36901 cagagaatct tctttttgtc tctggtttga ttgtgtccag tatgggcttt tccagtatga
    36961 tgagtgcagg agttatggga aagctaggtg acaaggtggg caatcatcgt ctcttggttg
    37021 tcgcccaatt ttattcagtc atcatctatc tcctctgtgc caatgcctct agcccccttc
    37081 aactaggact ctatcgattc ctctttggct tgggaaccgg tgccttgatt cccggggtta
    37141 atgccctact cagcaaaatg actcccaaag ccggcatttc gagggtcttt gccttcaatc
    37201 aggtattctt ttatctggga ggtgttgttg gtcccatggc aggttctgca gtagcaggtc
    37261 aatttggcta ccatgctgtc ttttatgcga caagcctttg tgttgccttt agttgtctct
    37321 ttaacctgat tcaatttcga acattattaa aagtaaagga aatctagtgc gagtaaaaat
    37381 taatctcaaa tgctcctctt gtgacagtat caattaccta accagtaaaa attcaaaaac
    37441 ccatccagac aagattgagg ttttaaagta ttgtcccaag gaaagaaaag taaccctaca
    37501 tcttgaatct aagtagattt tatggtaaaa taatagggat tttaaggagt ttgatatgta
    37561 taacctatta ttaaccattt tattagtatt atctgttgtg attgtgattg caattttcat
    37621 gcaaccaacc aaaaaccaat ccagcaatgt atttgatgcc agttcaggtg atttgtttga
    37681 acgcagtaaa gctcgcggtt ttgaagctgt aatgcagcgt ttgacaggga ttttagtctt
    37741 tttctggcta gccattgcct tagcattgac ggtattatca agtagataag aaaataatgg
    37801 gcaggactag gtctttgcct ctttttattt ttaaaggatg tttgagaagg ttttacagta
    37861 aaagaaaatt aaaaaatcta gaaagaaaat atgaaagata gaataaaaga atatttacaa
    37921 gacaagggaa aggtgactgt taatgatttg gctcaggctt tgggaaaaga cagttccaag
    37981 gattttcgtg agttgattaa aaccttgtcc ttaatggaaa gaaagcacca aattcgtttt
    38041 gaagaagatg gcagtctgac cttggaagcc aagaaaaaac atgagattac cctcaagggg
    38101 atttttcatg cccataaaaa tggttttggt tttgtcagcc tagaagggga agaggacgac
    38161 ctttttgtag ggaaaaatga tgtcaactat gctattgatg gtgataccgt cgaggtagtg
    38221 attaagaaag tcgctgaccg caataaggga acagcagcag aagccaaaat tattgatatc
    38281 ctagaacaca gtttgacaac agttgtcggg caaatcgttc tggatcagga aaagcctaag
    38341 tatgctggct atattcgttc aaaaaatcag aaaatcagtc aaccgattta tgttaagaaa
    38401 ccagccctaa agttagaagg aacagaagtt ctcaaggtct ttatcgataa atacccaagc
    38461 aagaaacatg atttctttgt cgcgagtgtt ctcgatgtgg tgggacactc aacggatgtc
    38521 ggaattgatg ttcttgaggt cttggaatca atggacattg tatccgagtt tccagaagct
    38581 gttgtcaagg aagcagaaag tgtgcctgat gctccgtctc aaaaggatat ggaaggtcgt
    38641 ctggatctaa gagatgaaat tacctttacc attgacggtg cggatgccaa ggacttggac
    38701 gatgcagtgc atatcaaggc tctgaaaaat ggcaatctgg agcttggggt tcacatcgca
    38761 gatgtttcct attatgtgac cgagggttct gcccttgaca aggaagccct taaccgtgcg
    38821 acttctgttt acgtgacaga ccgagtggtg ccaatgcttc cagaacgact atcaaatggc
    38881 atctgctctc tcaatcccca agttgaccgc ctgacccagt ctgctattat ggagattgat
    38941 aaacatggtc gtgtggtcaa ctataccatt acacaaacag ttatcaagac cagttttcgt
    39001 atgacctata gcgatgtcaa tgatatccta gctggcgatg aagaaaagag aaaagaatac
    39061 cataaaattg taccaagtat cgaactcatg gccaagcttc atgaaacttt agaaaacatg
    39121 cgtgtgaaac gtggagctct caattttgat accaatgaag cgaagatttt agtggataaa
    39181 caaggtaagc ctgttgatat cgttcttcgg cagcgtggca ttgccgagcg gatgattgag
    39241 tcttttatgt tgatggctaa tgaaacagtt gccgaacatt tcagcaagtt ggatttgcct
    39301 tttatctacc gaattcacga ggagcctaag gctgaaaagg ttcagaagtt tattgattat
    39361 gcttcgagtt ttggcttgcg catttatggg actgccagtg agattagcca ggaggcactt
    39421 caagacatca tgcgtgctgt tgagggagaa ccttatgcag atgtattgtc catgatgctt
    39481 cttcgctcta tgcagcaggc tcgttattcg gagcacaatc acggccacta tggactagct
    39541 gctgactatt atactcactt taccagtcca attcgtcgtt atccagacct tcttgttcac
    39601 cgtatgattc gggattacgg ccgttctaag gaaatagcag agcattttga acaagtgatt
    39661 ccagagattg cgacccagtc ttccaaccgt gaacgtcgtg ccatagaagc tgagcgtgaa
    39721 gtcgaagcca tgaaaaaggc tgagtatatg gaagaatacg tgggtgaaga gtatgatgca
    39781 gttgtatcaa gtattgtcaa attcggtctc tttgtcgaat tgccaaacac agttgaaggc
    39841 ttgattcaca tcactaatct acctgaattt tatcatttca atgagcgtga tttgactctt
    39901 cgtggagaaa aatcaggtat cactttccga gtgggtcagc agatccgtat ccgtgttgaa
    39961 agagcggata aaatgactgg agagattgat ttttcattcg tacctagtga gtttgatgtg
    40021 attgaaaaag gcttgaaaca gtctagtcgt agtggcagag ggcgtggttc aaatcgtcgt
    40081 tcggataaga aggaagacaa gagaaaatca ggacgctcaa atgataagcg taaacattca
    40141 caaaaagaca agaagaaaaa aggaaagaaa cctttttaca aggaagtagc taagaaagga
    40201 gccaagcatg gcaaagggcg agggaaaggt cgtcgcacaa aataaaaagg cacgccacga
    40261 ctatacaatc gtagatacgc tagaggcagg gatggtcctg actggaactg aaatcaagag
    40321 tgtacgagct gctcgaatta atctcaagga tggctttgct caagtgaaaa atggagaagt
    40381 ttggctgagc aatgttcata tcgcacctta cgaagagggc aatatctgga accaggaacc
    40441 agaacgtcgt cgtaaactcc tgctccataa aaagcaaatt caaaaattgg aacaagagac
    40501 caaagggaca ggaatgacct tagttcccct taaggtctat ataaaagatg gctacgctaa
    40561 gcttctttta ggacttgcca aagggaagca tgactatgac aaacgggagt ctatcaaacg
    40621 tcgtgagcaa aatcgagata tcgcgcgtgt gatgaaagct gttaatcagc gataaaaaga
    40681 ggaattgaaa atggaaaaat tagttgccta taaacgcatg cctttgtgga ataaacaaac
    40741 aatgcctgaa gctgttcagc aaaagcacaa tacaaaagtt gggacttggg ggaaaattac
    40801 tgtcttgaag ggagctctca agtttattga attgacagaa gaaggggaag ttctagctga
    40861 acacctcttt gaagcagggg cagacaatcc aatggcccag cctcaagcct ggcaccgagt
    40921 ggaagctgcc acagatgatg tggaatggta cttggaattt tattgtaaac ctgaggatta
    40981 ttttgctaaa aaatacaata ccaaacctgt tcattcagag gtcctagagg ccatgcagac
    41041 agtgaaacaa gggaaagctt tggatttggg ttgtggtcag gggcgtaatt ctctttttct
    41101 agcccagcaa gattttgatg tgacggctgt agatcaaaat ggactggctc ttgaaatctt
    41161 gcaaagcatt gtggagcagg aagatttgga catacctgtt gacctttacg atatcaattc
    41221 agctagcatt ggacaagaat atgattttat cgtttcaaca gttgttctca tgtttctaca
    41281 agcggaccgc attccagcta ttattcaaaa tatgcaggag aaaaccagtg ttggtggtta
    41341 caaccttatc gtttgtgcca tggacacgga ggactatcct tgctcggtta acttcccatt
    41401 cacctttaaa gaaggagaac tggcagacta ttacaaggat tgggaattgg ttaagtacaa
    41461 tgaaaatcca ggccatttgc atcgtcgcga tgagaatggc aatcgtattc aactacgctt
    41521 tgcgacctta ctagctaaga aaatcaagta aacacacatg aagattagga attttcctga
    41581 tcttttttct tttttacgaa tgatatagaa aaggagggaa ttcatgtttg ttgcgagaga
    41641 tgctagggga gaattggtaa atgtgttaga ggataaactt gagaagcaag catacacctg
    41701 cccagcttgt ggaggccagc tccatttgcg tcaaggacca agtgtacgga cgcattttgc
    41761 ccataaatcc ttaaaagact gtgatttttt ctttgaaaat gaaagtccag aacacctggc
    41821 caataaggaa tccctctatc actggttaaa aaaagagaca aaggttcaat tagagtaccc
    41881 gctttcagaa cttaaacaga ttgcggatgt atttgtaaat ggcaatctag ctctagaagt
    41941 tcagtgtagt cccttgcctc agaaagtcct taaagagcga agtgagggat atcgtagtca
    42001 gggttaccaa gtactgtggt tgctgggtca aaaactgtgg ctcaaggagc gtttgactcg
    42061 tctacagcaa ggttttcttt atttcagtca aaacatgggc ttttatgttt gggaattaga
    42121 caaggaaaaa caagttttaa gactcaaata cctgatttac caggatctcc gcggtaaact
    42181 ccattatcaa atcaaggaat tttcctatgg tcaaggtagt ttattggaaa tattgcgtct
    42241 tccctataag agacaaaaaa tatctcattt tacagtttct gaggacaagg acatctgtcg
    42301 ctatatccgg caacaacttt attatcaaaa tctcttttgg atgaaagaac aagcagaagc
    42361 ctatcaaaag ggagaaaata tcctgactta tggactgaaa gaatggtatc cacaaattcg
    42421 accaatagtg ggcaaatttt tccagattga acaagacttg actagctatt atcagcactt
    42481 ttatacctat taccaaaaaa atcctcaaaa tgattggcaa aagctttatc caccagcctt
    42541 ttatcagcaa tatttcttga aaaatatggt agaatagaaa ggatggagga atctaatggt
    42601 attacaaaga aatgaaagaa atgaaataaa tgaaaaagat acatgggatc tatcaacgat
    42661 ctacccaact gaccaggctt gggaagaagc cttaaaagat ttaacagaac aattggagac
    42721 agtagcccag tatgaaggcc atctcttgga tagtgcggat aacctactag aaatcactga
    42781 attttctctt gaaatggaac gccagatgga gaagctttac gtttatgctc atatgaagaa
    42841 tgaccaggat acacgtgaag ctaagtatca agagtactat gccaaggcca tgacactcta
    42901 cagccagtta gaccaagcct tttcattcta tgatcctgaa tttatggaga ttagcgaaaa
    42961 gcagtatgct gactttttag aagctcaacc aaagctgcag gtttatcaac actattttga
    43021 caagctcttg caaggcaagg atcacgttct ttcacaacgt gaagaagaat tattggctgg
    43081 agctggagaa atctttggtt cagcaagtga aaccttcgct atcttggaca atgcggatat
    43141 tgtgttccct tatgtcctag acgatgatgg taaagaagtt cagctatctc atgggactta
    43201 cacacgtttg atggagtcta aaaaacgtga ggttcgccgt ggtgcctatc aagctcttta
    43261 tgcgacttac gaacaattcc aacacaccta tgccaaaacc ttgcaaacca atgttaaggt
    43321 gcaaaattac cgtgctaaag ttcgtaacta caagagtgct cgtcatgcag ccctcgcagc
    43381 gaattttgtt ccagaaagtg tttatgacaa tttggtagca gcagttcgca agcatttgcc
    43441 actcttacat cgctatcttg agcttcgttc aaaaatcttg gggatttcag atctcaagat
    43501 gtacgatgtc tacacaccgc tttcatctgt tgaatacaat tttacctacc aagaagcctt
    43561 gaaaaaagca gaagatgctt tggcagtctt gggtgaggat tacttgagcc gtgtcaaacg
    43621 tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa ggcaagcgtt caggtgccta
    43681 ctctggtggt tcttacgata ccaatgcctt tatgcttctc aactggcagg acaatctgga
    43741 caatctcttt actcttgttc atgaaacagg tcacagtatg cattcaagct atactcgtga
    43801 aactcagcct tatgtttacg gagattactc tatctttttg gctgagattg cctcaactac
    43861 caatgaaaat atcttgacgg agaaattatt ggaagaagtg gaagacgacg caacacgctt
    43921 tgctattctc aataacttcc tagatggttt ccgtggaaca gttttccgcc aaactcaatt
    43981 tgctgagttt gaacacgcca ttcaccaagc agatcaaaat ggggaggtct tgacaagcga
    44041 tttcctaaat aaactctacg cagacttgaa ccaagagtat tatggtttga gtaaggaaga
    44101 caatcctgaa atccaatacg agtgggctcg cattccacac ttctactata actactatgt
    44161 atatcaatat tcaactggct ttgcggccgc ctcagccttg gctgaaaaaa ttgtccatgg
    44221 tagtcaagaa gaccgtgacc gctatatcga ctacctcaag gcaggtaagt cggactatcc
    44281 acttaatgtc atgagaaaag ctggtgttga tatggagaag gaagactacc tcaacgatgc
    44341 ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc cttgttgaaa aattaggatt
    44401 ggcataaaat ggttgaatcg tatagtaaga atgctaacca taacatgcgt cgtcctgtcg
    44461 tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa gcaggtcaca ggtttcttga
    44521 aagaattgga agactttgcc cgcaaggaaa atattcctat tattccccat gaaacggttg
    44581 cttatttccg ttttcttatg gaaaccatgc agcctaaaaa tattctggaa attgggacgg
    44641 ctatcggttt ttcagctctc ttgatggctg aacatgcgcc aaatgctaag attacaacta
    44701 ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa ttttgcccag tttgacagtc
    44761 gcaagcaaat cactctccta gagggagatg cggtggatgt cttatctaca ctgacagagt
    44821 cttatgattt cgtctttatg gattctgcca agtctaaata catcgtcttt ctgccagaaa
    44881 tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga tgatattttt caaggtggtg
    44941 atgttgccaa ggatattatg gaagtccgtc gtggtcagcg aaccatttat cgaggccttc
    45001 aaaaattatt ttatgcaacc ttagacaatc cagaactcac cgcaacatta gtgcctttag
    45061 gagatggtat tctcatgctt cgtaaaaatg tagcagatgt tcaactgtct gaaagcgaat
    45121 gattttcaga aaaatttaag aaaaaatagt aaaatagata gagtaacact tatctcaaag
    45181 gagtagacat gaagaaaaaa ttattggcag gtgccatcac actattatca gtagcaactt
    45241 tagcagcttg ttcgaaaggg tcagaagggg cagaccttat cagcatgaaa ggggatgtca
    45301 tcacagaaca tcaattttat gagcaagtga aaaacaaccc ttcagcccaa caagtcttgt
    45361 taaatatgac catccaaaaa gtttttgaaa aacaatatgg ctcagagctt gatgataaag
    45421 aggttgatga tactattgcc gaagaaaaaa aacaatatgg cgaaaactac caacgtgtct
    45481 tgtcacaagc aggtatgact cttgaaacac gtaaagctca aattcgtaca agtaaattag
    45541 ttgagttggc agttaagaag gtagcagaag ctgaattgac agatgaagcc tataagaaag
    45601 cctttgatga gtacactcca gatgtaacgg ctcaaatcat ccgtcttaat aatgaagata
    45661 aggccaaaga agttctcgaa aaagccaagg cagaaggtgc tgattttgct caattagcca
    45721 aagataattc aactgatgaa aaaacaaaag aaaatggtgg agaaattacc tttgattctg
    45781 cttcaacaga agtacctgag caagtcaaaa aagccgcttt cgctttagat gtggatggtg
    45841 tttctgatgt gattacagca actggtacac aagcttacag tagccaatat tacattgtaa
    45901 aactcactaa gaaaacagaa aaatcatcta atattgatga ctacaaagaa aaattaaaaa
    45961 ctgttatctt gactcaaaaa caaaatgatt caacatttgt tcaaagcatt atcggaaaag
    46021 aattgcaagc agccaatatc aaggttaagg accaagcctt ccaaaatatc tttacccaat
    46081 atatcggtgg tggagattca agctcaagca gtagtacatc aaacgaatag tccaaatcaa
    46141 tgagtcaggg aaaaaaactc gacttcagga aaaaatgaag caaacattcc cacaataaaa
    46201 cgcatagtac aaggtttgta ctgcccccaa aagttagaca attaatttat ctgaaggatt
    46261 tagttctgta ttg
//
LOCUS       DAVHIU010000004        39320 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVHIU010000004 DAVHIU010000000
VERSION     DAVHIU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.004"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..71
                     /locus_tag="VVN06_000176"
     CDS             <1..71
                     /locus_tag="VVN06_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU5561794.1"
                     /translation="IPAMSAPVFDNIEAAIKEHGLS"
     gene            119..1594
                     /locus_tag="VVN06_000177"
     CDS             119..1594
                     /locus_tag="VVN06_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU5561795.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            1989..8690
                     /locus_tag="VVN06_000178"
     CDS             1989..8690
                     /locus_tag="VVN06_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5561796.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8938..9795)
                     /locus_tag="VVN06_000179"
                     /pseudo
     CDS             complement(8938..9795)
                     /locus_tag="VVN06_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10006..10365)
                     /locus_tag="VVN06_000180"
     CDS             complement(10006..10365)
                     /locus_tag="VVN06_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU5561797.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMVVELFLMK"
     gene            10457..11414
                     /locus_tag="VVN06_000181"
                     /pseudo
     CDS             10457..11414
                     /locus_tag="VVN06_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11398..11955
                     /locus_tag="VVN06_000182"
     CDS             11398..11955
                     /locus_tag="VVN06_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5561798.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11948..12202
                     /locus_tag="VVN06_000183"
     CDS             11948..12202
                     /locus_tag="VVN06_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561799.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12214..14268
                     /locus_tag="VVN06_000184"
     CDS             12214..14268
                     /locus_tag="VVN06_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5561800.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14447..15307
                     /locus_tag="VVN06_000185"
     CDS             14447..15307
                     /locus_tag="VVN06_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5561801.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            15518..16228
                     /gene="ccdA2"
                     /locus_tag="VVN06_000186"
     CDS             15518..16228
                     /gene="ccdA2"
                     /locus_tag="VVN06_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU5561802.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16248..16814
                     /locus_tag="VVN06_000187"
     CDS             16248..16814
                     /locus_tag="VVN06_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU5561803.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16825..17937
                     /gene="msrB"
                     /locus_tag="VVN06_000188"
     CDS             16825..17937
                     /gene="msrB"
                     /locus_tag="VVN06_000188"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5561804.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18295..19032
                     /locus_tag="VVN06_000189"
     CDS             18295..19032
                     /locus_tag="VVN06_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5561805.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPYLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19029..20720
                     /locus_tag="VVN06_000190"
     CDS             19029..20720
                     /locus_tag="VVN06_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5561806.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20836..21399
                     /locus_tag="VVN06_000191"
     CDS             20836..21399
                     /locus_tag="VVN06_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU5561807.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21421..27129
                     /locus_tag="VVN06_000192"
     CDS             21421..27129
                     /locus_tag="VVN06_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5561808.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVEAEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKGPEQPVQPTQAEQPRIPKDSSQPEDPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEV
                     VPDDTKYVDEEVVERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPAVPKVVKKG
                     TKPVKGTAVETREEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKT
                     NEAPTVEETVLQAPQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVE
                     IKSIKVALKDNTGTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETETL
                     EDKEVQLDLKKVEIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKT
                     TRLAVSSVEEVVVDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYS
                     FNELITDMQKNPTGTFKLGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTAR
                     PLFNNIVGGTVKNINLNNVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVS
                     GFIDKIDSGGRLVNVAFIGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAK
                     AAGISYWSQNSGDNNAVGRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVA
                     MMKVKNGEIFYGSHDIDTDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQE
                     EADKKIKELGITADKFTIIDPIVNKLNNIKQKADTYKDTQDYKADRELTYRNIEKLQP
                     FYNKEWIVDQGNKVPSNSKLLTTEVLSVTGIKSGQFVTDLSDIDKIMIHYSDGAKEEL
                     NVTRQESNVQQVREYSITDLDILYTPNMVEKDRAQLMTDVKSKLSSVELESDGVRQLL
                     VKRDTKKDANANSVGRQNGYIRDLFLEESFSEVKANLDKLVKQILENEDHQLNDNELA
                     ERALLKKVEDNKAKIMMGLAYLNQYYAFKYDELSIKDIMMFKPDFYGKTASVIDRLIN
                     IGSAENNLKGDRTQDAYRGIISGATGKGSLHDFLTYNMKLFTNETDINVWFKKAIEKN
                     AYVVEQPSTNPAFANKKYRLYEGINNGQHGRMILPLLNLKNAHLFMISTYNTISFSSF
                     EKYGKDTDEKREKFKSEINKRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDN
                     HNSPNGWASRHGHIDGKPDYAPIREFFGRINKYHGYKYGYGAYAYIFAAPQPMDAVYF
                     VMTDLISDFGTSAFTHETTHVNDRMAYYGGHWHREGTDLEAFAQGMLQTPSVSNPNGE
                     YGALGLNMAYERQNDGNQWYNTNPNDLTTRAEIDNYMKGFNDTLMLLDYLEGEAVLNK
                     ANQDLNNAWFKKVDKQLRGASTKNQYDKVRDLTADEKNITLNSVDDLVDNNFMTKHGP
                     GNNVYDPTGFGTAYVTVPITAGIYGGNTSEGAPGSMSFKHNTFRMWGYYGYEKGFLNY
                     ASNMLKNESRQAGHNTLGDDFIIKKVSDNKFSTLEDWKKAYFKEVVDKAKAGFNPVTI
                     DGTTYSSYDDLKNAFAAAVEKDKATLKNGSVKSDNTVALKEKIYKKLLQQTDSFKTSI
                     FR"
     gene            27190..28911
                     /gene="pabB"
                     /locus_tag="VVN06_000193"
     CDS             27190..28911
                     /gene="pabB"
                     /locus_tag="VVN06_000193"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU5561809.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLTVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKDQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            28912..29529
                     /locus_tag="VVN06_000194"
     CDS             28912..29529
                     /locus_tag="VVN06_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5561810.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29579..30577
                     /locus_tag="VVN06_000195"
     CDS             29579..30577
                     /locus_tag="VVN06_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5561811.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYARNAWQGN
                     YYLKSDGKMAKGEWVYDATYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKTYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            30704..31663
                     /locus_tag="VVN06_000196"
     CDS             30704..31663
                     /locus_tag="VVN06_000196"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU5561812.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31760..32599
                     /locus_tag="VVN06_000197"
     CDS             31760..32599
                     /locus_tag="VVN06_000197"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU5561813.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32646..32816)
                     /locus_tag="VVN06_000198"
     CDS             complement(32646..32816)
                     /locus_tag="VVN06_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU5561814.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            32937..33821
                     /gene="miaA"
                     /locus_tag="VVN06_000199"
     CDS             32937..33821
                     /gene="miaA"
                     /locus_tag="VVN06_000199"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5561815.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33814..35052
                     /gene="hflX"
                     /locus_tag="VVN06_000200"
     CDS             33814..35052
                     /gene="hflX"
                     /locus_tag="VVN06_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5561816.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35045..35668
                     /locus_tag="VVN06_000201"
     CDS             35045..35668
                     /locus_tag="VVN06_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU5561817.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35683..36612
                     /locus_tag="VVN06_000202"
     CDS             35683..36612
                     /locus_tag="VVN06_000202"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU5561818.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36633..37388
                     /locus_tag="VVN06_000203"
     CDS             36633..37388
                     /locus_tag="VVN06_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5561819.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37442..38350)
                     /locus_tag="VVN06_000204"
     CDS             complement(37442..38350)
                     /locus_tag="VVN06_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5561820.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38414..38683
                     /locus_tag="VVN06_000205"
     CDS             38414..38683
                     /locus_tag="VVN06_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU5561821.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38683..39063
                     /locus_tag="VVN06_000206"
     CDS             38683..39063
                     /locus_tag="VVN06_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5561822.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39152..>39320
                     /locus_tag="VVN06_000207"
     CDS             39152..>39320
                     /locus_tag="VVN06_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561823.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNI"
BASE COUNT        12109 a         7317 c         8777 g        11117 t
ORIGIN      
        1 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacatg
       61 gattaagcta attctaattt gttaacattc atataacata aaaaagggag gaactgttat
      121 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
      181 tgtcatcatg ttgatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
      241 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
      301 tatgctaaat ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca
      361 attgagtatt acagacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg
      421 gacactatac ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa
      481 aaccgataca cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt
      541 gattaaatgg tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac
      601 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
      661 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
      721 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
      781 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
      841 attcttcatc ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat
      901 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
      961 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     1021 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc gaggacgtaa
     1081 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     1141 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     1201 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     1261 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     1321 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     1381 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     1441 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     1501 agtagtcttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     1561 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     1621 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctaaaaacac agttttgagg
     1681 ttgtagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
     1741 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagctcaa
     1801 aacatggttt tgaggttgta gatgaaactg acgaagtcag taatcatatc tacggcaagg
     1861 cgacgctgac gaagtttgaa ttggattttc gcagagtgta aattgttgaa actaggttgt
     1921 cataccatgt ataccacttg ggctttggta gtagctaact gcactaaata taatataagg
     1981 agggttttat ggggaaaggc cattggaatc ggaaaagagt ttatagcatt cgtaagtttg
     2041 ctgtgggagc ttgctcagta atgattggga cttgtgcagt tctattagga ggaaatatag
     2101 ctggagaatc tgtagtttat gcggatgaaa cacttattac tcatactgct gagaaaccta
     2161 aagaggaaaa aatgatagta gaagaaaagg ctgataaagc tttggaaact aaaaatgtag
     2221 ttgaaaggac agaacaaagt gaacctagtt caactgaggc tattgcatct gagaagaaag
     2281 aagatgaagc cgtaactcca aaagaggaaa aagtgtctgc taaaccggaa gaaaaagctc
     2341 caaggataga atcacaagct tcaagtcaag aaaaaccgct caaggaagat gctaaagctg
     2401 taacaaatga agaaatgaat caaatgattg aagacaggaa agtggatttt aatcaaaatt
     2461 ggcactttaa actcaatgca aattctaagg aagccattaa acctgatgca gacgtatcta
     2521 cgtggaaaaa attagattta ccgtatgact ggagtatctt taacaatttc gatcatgaat
     2581 ctcctgcaca aaatgaaggt ggacaactca acggtgggga agcttggtat cgcaagactt
     2641 tcaaactaga tgaaaaagac ctcaagaaaa atgttcgcct tacttttgat ggcgtctaca
     2701 tggattctca agtttatgtc aatggtcagt tagtggggca ttatccaaat ggttataacc
     2761 agttctcata tgatatcacc aaataccttc acaaagatgg tcgtgagaat gtgattgctg
     2821 tccatgcagt caacaaacag ccaagtagcc gttggtattc aggaagtggt atctatcgtg
     2881 atgtgacttt acaagtgaca gataaggtgc atgttgagaa aaatgggaca actattttaa
     2941 caccaaaact tgaagaacaa caacatggca aggttgaaac tcatgtgacc agcaaaatcg
     3001 tcaatacgga cgacaaagac catgaacttg tagccgaata tcaaatcgtt gaacgaggtg
     3061 gtcatgctgt aacaggctta gttcgtacag cgagtcgtac cttaaaagca catgaatcaa
     3121 caagcctaga tgcgatttta gaagttgaaa gaccaaaact ctggaccgtt ttaaatgaca
     3181 aacctgcctt gtacgaattg attacgcgtg tttaccgtga cggtcaattg gttgatgcta
     3241 agaaggattt gtttggttac cgttactatc actggactcc aaatgaaggt ttctctttga
     3301 atggtgaacg tattaaattc catggagtat ccttgcacca cgaccatggg gcgcttggag
     3361 cagaagaaaa ctataaagca gaatatcgcc gtctcaaaca aatgaaggag atgggagtta
     3421 actctatccg tacaacccac aaccctgcta gtgagcaaac cttgcaaatc gcagcagaac
     3481 taggtttact cgttcaggaa gaggcctttg atacgtggta tggtggcaag aaaccttatg
     3541 actatggacg tttctttgaa aaagatgcca ctcacccaga agctcgaaaa ggtgaaaaat
     3601 ggtctgattt tgacctacgt accatggtcg aaagaggcaa aaacaaccct gctatcttca
     3661 tgtggtcaat tggtaatgaa ataggtgaag ctaatggtga tgcccactct ttagcaactg
     3721 ttaaacgttt ggtcaaggtt atcaaggatg ttgataagac tcgctatgtt accatgggag
     3781 cagataaatt ccgtttcggc aatggtagcg gagggcatga gaaaattgct gatgaactcg
     3841 atgctgttgg atttaactat tctgaagata attacaaagc ccttagagct aagcatccaa
     3901 aatggttgat ttacggttca gaaacatcat cagcaacccg tacacgagga agttactatc
     3961 gccctgaacg tgaattgaaa catagcaatg gacctgagcg taattatgaa cagtcagatt
     4021 atggaaatga tcgtgtgggt tgggggaaaa cagcaaccgc ttcatggact tttgaccgtg
     4081 acaacgctgg ctatgctgga cagtttatct ggacaggtac ggactatatt ggtgaaccta
     4141 caccatggca caaccaaaat caaactcctg ttaagagctc ttactttggt atcgtagata
     4201 cagccggcat tccaaaacat gacttctatc tctaccaaag ccaatgggtt tctgttaaga
     4261 agaaaccgat ggtacacctt cttcctcact ggaactggga aaacaaagaa ttagcatcca
     4321 aagtagctga ctcagaaggt aagattccag ttcgtgctta ttcgaatgct tctagtgtag
     4381 aattgttctt gaatggaaaa tctcttggtc ttaagacttt caataaaaaa caaaccagcg
     4441 atgggcggac ttaccaagaa ggtgcaaatg ctaatgaact ttatcttgaa tggaaagttg
     4501 cctatcaacc aggtaccttg gaagcaattg ctcgtgatga atctggcaag gaaattgctc
     4561 gagataagat tacgactgct ggtaagccag cggcagttcg tcttattaag gaagaccatg
     4621 cgattgcagc agatggaaaa gacttgactt acatctacta tgaaattgtt gacagccagg
     4681 ggaatgtggt tccaactgct aataatctgg ttcgcttcca attgcatggc caaggtcaac
     4741 tggtcggtgt agataacgga gaacaagcca gccgtgaacg ctataaggcg caagcagatg
     4801 gttcttggat tcgtaaagca tttaatggta aaggtgttgc cattgtcaaa tcaactgaac
     4861 aagcagggaa attcacactg acagcccact ctgatctctt gaaatcgaac caagtcactg
     4921 tctttactgg taagaaagaa ggacaagaga agactgtttt ggggacagaa gtgccaaaag
     4981 tacagaccat tattggagag gcacctgaaa tgcctaccac tgttccgttt gtatacagtg
     5041 atggtagccg tgcagaacgt cctgtaacct ggtcttcagt agatgtgagc aagcctggta
     5101 ttgtaacggt gaaaggtatg gctgacggac gagaagtaga agctcgtgta gaagtgattg
     5161 ctcttaaatc agagctacca gttgtgaaac gtattgctcc aaatactgac ttgaattctg
     5221 tagacaaatc tgtttcctat gttttgactg atggaagtgt acaagagtat gaagtagata
     5281 gctgggagat tgccgaagaa gataaagcta agttagcaat tccaggttct cgtattcaag
     5341 cgaccggtta tttagaaggt caaccaattc atgcaaccct tgtggtagaa gaaggcaatc
     5401 ctgcagcacc tgtagtgcca actgttactg ttggaggtga agcagtaaca ggtcttacta
     5461 gtcgacaacc aatgcaatat cgtactctat cttatggtgc ccaattgcca gaagtcacag
     5521 caagtgctga aaatgctgat gtgacagttc ttcaagcaag cgcagcaaac ggcatgcgtg
     5581 cgagcatctt tattcagcct aaagatggtg gccctcttca aacctatgca attcaattcc
     5641 ttgaagaagc gccaaaaatt gctcacttga gtttgcaagt ggaaaaagct gacagtctca
     5701 aagaagacca aactgtcaaa ttgtcggttc gagctcacta tcaagatgga acgcaagctg
     5761 tattaccagc tgataaagta accttctcta caagtggtga aggggaagtc gcaattcgta
     5821 aaggaatgct tgagttgcat aagccaggag cagtcactct gaacgctgaa tatgagggag
     5881 ctaaaggcca agttgaactc actatccaag ccaatactga gaagaagatt gcgcaatcta
     5941 tccgtcctgt aaatgtagtc acagatttgc atcaaaaacc tactcttcca acaacagtaa
     6001 cggttgagta tgacaaaggt ttccctaaag ctcacaaagt cacttggcaa gctattccga
     6061 aagaaaaact agactcctat caaacatttg aagtactagg taaagttgaa ggaattgacc
     6121 ttgaagcgcg tgcaaaagtc tctgtagaag gtatcgtttc agttgaagaa gtcagtgtga
     6181 caactccaat cgcagaagca ccacaattac cagaaagcgt tcggacatat gattcaaatg
     6241 gtcacgtttc atcagctaag gttgcatggg atgcgattcg tatagagcaa tacgctaagg
     6301 aaggtgtctt tacagttaat ggtcgcttag aaggtactca attaacaact aaacttcatg
     6361 ttcgcgtatc tgctcaaact gagcaaggtg caaacatttc tgaccaatgg accggttcag
     6421 aattgccact tgcctttgct tcagattcaa atccaagcga cccagtttca aatgttaatg
     6481 acaagctcat ttcctacaat aaccaaccag ccaatcgttg gacaaactgg aatcgtagta
     6541 atccagaagc ttcagtcggt gttctgtttg gagattcagg tatcttgagc aaacgctccg
     6601 ttgataatct aagtgtcgga ttccacgaag accatggagt tggtgcaccg aagtcttatg
     6661 tgattgagta ttatgttggt aagactgtcc caacagctcc taaaaaccct agttttgttg
     6721 gtaatgagga ccatgtcttt aatgattctg ccaactggaa accagttact aatctaaaag
     6781 cccctgctca actcaaggct ggagaaatga accactttag ctttgataaa gttgaaacct
     6841 atgctgttcg tattcgcatg gttaaagcag ataacaagcg tggaacgtct atcacagagg
     6901 tacaaatctt tgcgaaacaa gttgcggcag ccaaacaagg acaaacaaga atccaagttg
     6961 acggcaaaga cttagcaaac ttcaaccctg atttgacaga ctactacctt gagtctgtag
     7021 atggaaaagt tccggcagtc acagcaaatg ttagcaacaa tggtctcgct accgtcgttc
     7081 caagcgttcg tgaaggtgag ccagttcgtg tcatcgcgaa agctgaaaat ggcgacatct
     7141 taggagaata ccgtctgcac ttcactaagg ataagaactt actttctcat aaaccagttg
     7201 ctgcggttaa acaagctcgc ttgctacaag taggtcaagc acttgaattg ccgactaagg
     7261 ttccagttta cttcacaggt aaagacggct acgaaacaaa agacctgaca gttgaatggg
     7321 aagaagttcc agcggaaaat ctgacaaaag caggtcaatt tactgttcga ggccgtgtcc
     7381 ttggtagtga ccttgttgct gaggtcactg tacgagtgac agacaaactt ggtgaggctc
     7441 tttcagataa ccctaactat gatgaaaaca gtaaccaggc ctttgcttca gcaaccaatg
     7501 atattgacaa aaactctcat gaccgcgttg actatctcaa tgacggagat cattcagaaa
     7561 atcgtcgttg gacaaactgg tcaccaacac catcttctaa tccagaagta tcagcgggtg
     7621 tgatcttccg tgaaaatggt aagattgtag aacggactgt tgcgcaagcc aaacttcact
     7681 tctttgcaga tagtggtacg gatgcaccaa ctaaactcgt tttagaacgc tatgtcggtc
     7741 cagagtttga agtgccaacc tactattcaa actaccaagc ctacgacgca gaccatccat
     7801 tcaacaatcc agaaaattgg gaagctgtgc cttatcgtgc ggataaagac attgaagccg
     7861 gtgatgaaat taacgttacc tttaaagctg tcaaagccaa agccatgaga tggcgtatgg
     7921 agcgtaaagc agataagagc ggtgttgcga tgattgagat gaccttcctt gcaccgagtg
     7981 aattgcctca agaaagcact caatcgaaga ttcttgtaga tggaaaagag cttgctgatt
     8041 tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg tcaacggcca aaagtctcag
     8101 ttgaagaaaa taatcaagta gcttcaactg tggtagatag tggagaagat agccttccag
     8161 tacttgttcg cctcgtttca gaaagtggaa aacaagttaa ggaataccgt atccagttga
     8221 ctaaggaaaa accagtttct gagaagacag ttgctgctgt acaagaagat cttccaaaac
     8281 tcgaatttgt tgaaaaagat ttggcctaca agacagttga gaaaaaagat tcaacactgt
     8341 atctaggtga aactcgtgta gaacaagaag gaaaaactgg taaagaacgt atctttacag
     8401 cgattaatcc tgatggaagt aaggaagaaa aactccgtga agtggtagaa gctccgacag
     8461 accgcatcgt cttggttgga accaaaccag tagctcaaga agctaaaaaa ccacaagtgt
     8521 cagaaaaagc agatacaaaa ccaattgatt caagtgaagc tagtcaaact aataaagccc
     8581 agttaccaaa tacaggtagt gcggcaagcc aagcagcagt agcagcaggt ttagctcttc
     8641 taggtttgag tgcaggatta gtagttacta aaggtaaaaa agaagactag aaattttgat
     8701 accttcttta tcattaaaga ccaaagaaaa actcttgtcc taaatagaag ataaagagtc
     8761 tgggacaaaa tagttctcaa ccacaaaaaa gctagatatt tccaattgcg gaactctagc
     8821 tttttaattt tgagtcgttc tcttattgta tagtagattg aaactagagt agtacacttc
     8881 tacttctaaa acattgttag aaatcgattt gactgtcctt attgatttgt cctgttctta
     8941 tttcatttca ctatgtttta ggaggttatt ggccataagt accaatccca tgtcaattct
     9001 cacttgacgc ttgcctctca gattacatct cttgtaaccc aaacaagcct ttaactgccc
     9061 aaagacaggt tccacatcaa tcttgcgttg agcgaaaatc tgtctacctt ggggagataa
     9121 aagcgcctga cattctttag ctttcaagtt ttgatagcgt tcgttcatat atagccccct
     9181 tttgaggggc tgattcaggt tcgtcggcat agtaaacctt gatttcctgt tgaaagtctg
     9241 tctgtgtttt ctgatgtttg gtatggtgaa aacgataata ccagccatca ggatgtgtgt
     9301 agctatcctc cttgtcatta tagtgccaat tcgctaagtt tttagctgac tgtttatacc
     9361 ctctcttttg ttccttatca aacatggcat atttaatcag aaggggagtg atttcttctt
     9421 gaaaatagac ttgtatctgt tcttgaagtt tggcggagaa tttctctgtc gttttcttcc
     9481 acacaaaact atacttgttg gcattggatt caatcttagt tccgtcaata aacaaacaat
     9541 ctaaggtcac taactcttcc atttttcaat ttttcgacca gagaaaatcc cttgtgaata
     9601 gacaaataga agagtagata caagcatttt agggtgataa gacgggcgat caaaggcatg
     9661 atagaaggcg tagaagtgat gttcctccaa ggtattcacc actttttcaa tagtaaagac
     9721 gagatggtct tgtggtaaga aggaactgat ttctagtggt aaagttgttt gatttgtgtt
     9781 atagtgaata tgcatgggat agcctttcta aatggtttat tgtgcaattc tattttacca
     9841 agactatcgc aaccatgcaa aaaagcaacg gcaaatccgt tgcttttttt ggagataaga
     9901 gactattgtc ccagactctt ttctttgtat aaaattgtat agtgttattg tctatactat
     9961 tttgtagaga aagcaaaaat ccctagaaaa tgcttaggga ttcgactatt tcattaagaa
    10021 caattcaaca accatctcta tatacatgat aagggttaat aaaaatcctg tacgccagaa
    10081 gaatttgatg aatttaggat agtaaaagct gcgttttttc aaaaggaaga aaaagacgag
    10141 aataatggct aggatcgaga gggctagccc cagtctaggg aggaaattat gggtaaaggt
    10201 tttagctgta atcaggtaat attcaaatac caagaccgga aaagccaaat ccgcaaagtt
    10261 tcgtcctagt ttttttaatc taaaaagttt ggttaggata atgcagacga ctaaggtcag
    10321 tatcaataat aaaatagatg ctaatttcat taaaatcata cccatattgt atcataaaaa
    10381 aaggctaatg gaaacgagaa acagaggaat ttataggaaa gtcaggcttt tgagattgtg
    10441 ggtgattttt gttataatga aagttataaa atcttataat accttgaatg attattatcg
    10501 aaaactcttt ggagaaaaga cttttaaagt ccctattgat gcgggatttg actgtcccaa
    10561 tcgtgatgga actgtggctc atggaggctg tactttttgt acggtttcag gttctggaga
    10621 tactattgta gccccagatg cgcctatccg tgagcaattt tataaggaaa ttggctttat
    10681 gcaccgtaag tggccggatg ttcagaagta tctggtttat tttcaaaatt ttaccaacac
    10741 ccatgaaaag gtggaagtta ttcgagagcg ttatgaacag gctatcaatg agccaggtgt
    10801 ggtaggaatc aatattggaa cgcgcccaga ctgtttacca gacgaaacca tcgaatattt
    10861 ggctgagtta tcggagtgca tgcatgtgac ggttgaatta ggcttgcaga ccacttatga
    10921 agcaacctct gacctgatta accgtgtgca ttcttatgaa ttgtaggtgg aaacggtcaa
    10981 gcgtttgaga aagtatccaa aaattgagat tgtttcccca tttgatcaat ggtttgcctg
    11041 gtgaaaccca tgagatgatg gttgaaaatg tccgccgttg tgtcacggat aacgatattc
    11101 aagggattaa actgcatttg ctccatctta tgacaaatac tcgtatgcaa cgagattacc
    11161 atgagggacg tttacaattg atgagtcagg acgaatatgt cagggtcatc tgtgaccaaa
    11221 tggaaattat tcccaaacat atcgtcatcc atcgaattac aggagatgcg cctagggata
    11281 tgctgattgg gcttatgtgg agcctcaata aatgggaagc tctcaacagc attgagatgg
    11341 agatgcgacg tcgtggaagt gttcaaggat gcaaggctgt aaaacaggag tttgaaaatg
    11401 aaaagaccac ttgagatggc acatgatttt ttggctgagg tcgtgacaaa agaggatgtc
    11461 gtagtggatg cgactatggg aaatggtcat gacacgcttt ttttagccaa gctagccaag
    11521 caagtctatg cctttgatat tcagaagcaa gccttggaaa agacccaaga gcgtttgcat
    11581 caggctggct tgacaaatgc ccagttaatc ttgcaaggcc atgagacact ggaccagttt
    11641 gtgacaaaag ctaaggcagg gatttttaat ctgggctatt tgccgtcagc tgataagtct
    11701 gtcatcaccc gaccgcagac aacgattgag gcattagaaa agctatgtgg cttacttgtc
    11761 aaaggtggac gaattgctat tatgatttac tatggtcatg aaggaggcga cctcgagaga
    11821 gatgctgtct tggattttgt aatccagttg aaccaacaag agtacacagc tgccatttac
    11881 cgaactttaa atcaagtcaa caacccgccg tttttagtga tgattgaaaa attagagaga
    11941 tatagacatg gataaacaat acctacgtga aaagctggat gccatgcgcc agaattttgt
    12001 tgaatcaacc caccacgaac gagcggtggg cgtgctagac caagcgcata tgagcaaaaa
    12061 aatgcttaaa atcaagaaaa aattagttgc tcttgaaatg gaacggtgcc agagaaaaat
    12121 tgagcacaaa gactgttcca agattgacca aaaaatcaaa gagcagaagg agatatttga
    12181 atcctgttgt aaaaaagatt aaggaggacg tgcgtggaat tactgattta cctcatccta
    12241 tttttactgg tcttgattgt ctcaagtaca accaataagc tcctgccctt tttgcctctc
    12301 cctttggtgc agattctttt gggaattgtg attggtctct ttttacccaa tactgacttt
    12361 catcttaata cggagttgtt tttggccctg gttatcggac ccttgctttt ccgagaggct
    12421 gaagaagcag atgttacggc tattttaaaa cactggcgaa tcattgttta tctcatattt
    12481 ccagtgattt ttatctcgac cctgagtttg ggtggcttgg cccatcttct ttggttcagc
    12541 cttcccttgg cagcttgctt ggctgttggg gcagcccttg gtcctacgga cttggtggcc
    12601 tttgcctctc tttcggagcg ttttagcttt cctaagcgcg tgtccaatat tcttaagggc
    12661 gaaggactct tgaatgatgc ttctggtttg gtggcttttc aggtagcttt gacagcttgg
    12721 acaactggag ctttttctct ggggcaagct agcagttcgc tcatcttttc aatcctaggc
    12781 ggttttttaa ttggcttttt aacagccatg accaatcgtt tcctccatag cttcttgcta
    12841 agtgtgagag caacggatat tgctagtgag cttttattag aattgagttt gcctctagtg
    12901 accttctttc tggcagaaga agtccatgtt tcagggatta ttgccgtcgt agttgctgga
    12961 attttaaagg caagtcgctt caagaaaatc acgctcctcg aagcccaagt ggatacggtg
    13021 accgagacgg tctggcatac agtgaccttt atgctcaacg gttctgtctt tgtgatttta
    13081 gggatggagt tggaaatgat agcagaacct atcttgacca atccaatcta taatcctcta
    13141 cttttattgc tatctctcat cgcccttacc tttgtcctct ttgtcattcg ttttattatg
    13201 atctatggct attatgccta tagaacccga cgcctcaaga aaaagctaaa taagtatatg
    13261 aaggacatgt ttctcttgac cttttcaggt gttaagggaa cggtgtccat tgctacgatt
    13321 ctcttgatac caagtaatct agaacaggag tatcctctct tgcttttcct tgttgcaggt
    13381 gtgacgcttg tcagcttttt aacaggtctc ttggtcttgc ctcatctttc tgatgaagag
    13441 gaagaaagca aggattatct catgcatatc gccattttga atgaagtaac gctagagttg
    13501 gaaaaagagt tggaagacac tagaaataaa cttcccctct atgcggctat tgacaattat
    13561 catggacgta ttgaaaatct cattttaagc caagaaaacc aggatgatca agaagactgg
    13621 gctgctttga aactcttgat tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa
    13681 gaggggaaca ttagcaatcg tgcttaccga gtttaccaac gttatctgaa aaatatagaa
    13741 caaggaatca atcgtaaact tgcctcaaga ctgacctatt attttcttgt ttccttgagg
    13801 attttacgtt ttctccttca tgaagttttt actctcggaa agaccttccg tagctggaag
    13861 aacaaggagc aaagccgtct ccgtgctctt gattatgacc aaattgcaga gctctatctt
    13921 gccaatacag agatgattat tgaaagtttg gaaaacctga agggagtcta cagacgctct
    13981 ttgattagtt ttatgcagga gtctcgtctt cgagaaacag ctattatcag cagtggtgcc
    14041 tttgtcgaac gggttatcaa tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc
    14101 tattatctgg agcgcaagtt gattttcgaa tacgaagaaa aacgattaat tacaactaag
    14161 tatgccaaga aattacgaca aaatgtaaat aacttagaga actattcctt gaaggaagct
    14221 gccaataccc tgccgtatga tatggtggaa ttggtaagaa gaaattagtt aatactcttc
    14281 gaaaatctct tcaaaccacg tcagcgtcgc cttggattat atatgtgact gacttcgtca
    14341 gtttcatcta caacctcaaa gcagggcttt gagcaacctg cggctagctt cctagtttgc
    14401 tctttgattt tcattgagta taagattata aatgaaggag tatgacatga aaaagtggtg
    14461 gaaagagctg atagacaaac ctttattaaa agcttttttg cattattatc aagcatcaga
    14521 tagtgagttg accagtgtcg cagtagccta ctattggttg atttcgattt tccccctgct
    14581 tttggtggtg gtcaatatcc tcccctattt tcagattcct gtgggagaat ttctgtgctt
    14641 tatgaaagac gttctgcccc caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt
    14701 tttgacgcaa ccttcaacag gtttattgag tttttctgtt ttatcggcgt tatggagttt
    14761 ttcaaaatct atgaatttct tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag
    14821 tcgtggactt atttcccatc agatgctaag tctcctagtc agttttggct tgcagctcct
    14881 ctttgcgttt gccttgtttt tgatcttatt tggtcagatg attttggctt tacttgctca
    14941 ttattggaca aaagatggca ttatctatca ggctttacaa gggttggcag gtcctctgat
    15001 ttacgccctc ctctttgcta tcttggtcat gctttattat tttcttccca atatatctaa
    15061 caggaaaatt agctataccc taccaggcag tgcctttgtt ctcttagtga ttttaggttt
    15121 actaactctg ttctctagtt atctcaatta ctatgtccat catttggtgg atgtccgaat
    15181 tttaggttcc gttcttcttg ttgttatgat gttttggttt atcttgattg ctaaaattgt
    15241 tatcctaggc gcggttatca atgccagtat gcagagtttg agagatccgg tctttaaaaa
    15301 agactaacta tttatccatt acaaaagcgc atagtatcaa aactttatag aaccttgata
    15361 ctatgcgctt ttgtgctttg acagtttttt gtatagtttt tgtccaagat ttctaaatca
    15421 tctgattaaa ttcctatttt cccctatcat tgtccctgtt ttttcgaaaa tggaaggagt
    15481 ataataaatc tatcaataaa atgataggag gaactttatg ggtcatattt tcttttttct
    15541 aagtgtcttt ttggcaggga ttctatcctt cttttctcct tgtatcttac ctttgttacc
    15601 ggtctataca ggagtgttac tagatgataa ggatggtgct caggcttcta gcggcaaatt
    15661 ttcaatctca gttactagtt tattacgaac tctggccttt atagcaggaa tttcctttat
    15721 atttattttg ttgggctatg gagctggttt tttaggcgat ttgctttatg cttcttggtt
    15781 ccaatatctt actggggcaa ttattatcct tcttggtttg caccaaatgg agattctaca
    15841 ctttaagggg ctttataagg aaaagaggct acaactgcaa ggacaggggc aaaatggtaa
    15901 gggctatagt caggcatttt tattgggctt gacctttagt tttgcttgga cgccttgcgt
    15961 ggggccggtt ctggggtctg ttttggcctt ggcggcttca ggtggttcag gagcttggca
    16021 gggagctggt ctcatgttgg tgtatacgct gggcttggcg ctaccattct tgcttctagc
    16081 tctgacctct agttatgttt tgaaacattt ccgaaaactt catccctatc tcggaatcct
    16141 caaaaaagtg ggtggttttc tcattattgt gatgggactc ttggttctgt ttggaaatgc
    16201 ttcaatttta agtcaattat ttgaataaaa tggaaaggaa tatcaatatg aaaaaatggc
    16261 aaacatgtgt tcttggagca ggttcgctcc tttgtttgac ggcttgttca ggcaagtccg
    16321 tgactagtga acaccaaacg aaagatgaaa tgaagacgga gcagacagct agtaaaacaa
    16381 gcgcagctaa agggaaagag gtggctgatt ttgaattgat gggagtagat ggcaagacct
    16441 accgtttatc tgattacaag ggcaagaaag tctatctcaa attctgggct tcttggtgtt
    16501 ccatctgtct ggctagtctt ccagatacgg atgagattgc taaagaagct ggtgatgact
    16561 atgtggtctt gacagtagtg tcaccaggac ataagggaga gcaatctgaa gcggacttta
    16621 agaattggta taagggattg gattataaaa atctcccagt cctagttgac ccatcaggca
    16681 aacttttgga aacttatggt gtccgttctt acccaaccca agcctttata gacaaagaag
    16741 gcaagctggt caaaacacat ccaggattca tggaaaaaga tgcaattttg caaactttga
    16801 aggaattagc ctaggaggtg tcttatgaat gataagttaa aaatcttctt gttgctagga
    16861 gtattttttc tagccataac cggtttctat gttctattga tacgaaatgc agggcagaca
    16921 gatgcctcgc aaattgaaaa ggcggcagtt agccaaggag gaaaagcagt gaaaaaaaca
    16981 gaaattagtg aagacgcaga cttgcacgaa atttatctag ctggaggttg tttctgggga
    17041 gtggaggaat atttctcacg cgttcccggg gtgacggatg ccgtttcagg ctatgcaaat
    17101 ggtagaggag aaacaaccaa gtacgaatta attaaccaaa caggtcatgc agaaaccgtc
    17161 catgtcacct atgatgccaa gcaaatttct ctcaaggaaa tcctgcttca ctatttccgc
    17221 attatcaatc caaccagcaa aaataaacaa ggaaatgatg tggggaccca gtaccgtact
    17281 ggtgtttatt acacagatga caaggatttg gaagtgatta accaagtctt tgatgaggtg
    17341 gctaagaaat acgatcaacc tctagcagtt gaaaaggaaa acttgaagaa ttttgtggtg
    17401 gctgaggatt accatcaaga ctatctcaag aaaaatccaa atggctactg ccatatcaat
    17461 gttaatcagg cggcctatcc tgtcattgat gccagcaaat atccaaaacc aagtgatgag
    17521 gaattgaaaa agaccctgtc acctgaggag tatgcagtta cccaggaaaa tcaaacagaa
    17581 cgagctttct caaaccgtta ctgggataaa tttgaatccg gtatctatgt ggatatagca
    17641 actggggaac ctctcttttc atcaaaagac aaatttgagt ctggttgtgg ctggcctagt
    17701 tttacccaac ccatcagtcc agatgttgtc acctacaagg aagataagtc ctacaatatg
    17761 acgcgtatgg aagtgcggag ccgagtagga gattctcacc ttgggcatgt ctttacggat
    17821 ggtccacagg acaagggcgg cttacgttac tgtatcaata gcctctctat ccgctttatt
    17881 cccaaagacc aaatggaaga aaaaggctac gcttatttac tagattatgt tgattaatac
    17941 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac cgtatgtagg gttactgact
    18001 tcgtcagttt tatctacaac ctcaaagcta tactttgagc tgacttcgtc agttttatct
    18061 acaacctcaa agcggtactt tgagctgact tcgtcagttt tatctacaac ctcaaagcgg
    18121 tactttgagc tgacttcgtc agttttatct acaacctcaa agcggtactt tgagaaacct
    18181 gtggctatct tcctagttta ctttttgatt tttattgagt ataagaggtc tttcctaagc
    18241 agttagagga gaattttgct atactgatac tagtaagtgg taaaggagca gagcatgacc
    18301 tacacaatct taatcgtaga agatgaatat ctggtaagac aaggtttgac taaactggtc
    18361 aatgtagcag cctacgatat ggaaatcatc ggtcaggctg aaaatggaag gcaggcttgg
    18421 gaattgatcc aaaagcaggt gccagatatc attttaaccg atatcaacat gccttatcta
    18481 aatggcatcc agttggccag tctggtacga gaaacctatc ctcaggttca tttggtcttt
    18541 ttaacaggtt acgatgattt tgattatgcc ttgtctgctg tcaaactagg tgtggacgac
    18601 tacctgctca aacccttttc tcgtcaggat attgaggaaa tgttggggaa aatcaaacaa
    18661 aaactagaca aggaagagaa agaagagcag ttacaagatt tattgactaa taggtttgaa
    18721 ggaaacatgg cccagaaaat ccagtctcat ctggctgata gccaatttag tttaaagtct
    18781 ttagccagtg acttaggttt tagtccgacc tatctgagtt ccttgattaa gaaagagttg
    18841 ggcttgcctt ttcaggatta tctggtgaga gaacgtgtta aacaagccaa gctcttgctt
    18901 ttaactacag atctgaagat ttatgagatc gcagagaagg ttggttttga agatatgaac
    18961 tattttaccc aacgttttaa gcagattgca ggtgtgacac ctcgtcagtt taagaaggga
    19021 gaagaccgat gaagcgttct tctcttttag ttagaatggt tatttccatc tttctggtct
    19081 ttctcattct cctagctctg gttggaactt tctactatca atcaagttct tcagccattg
    19141 aggccaccat tgagggcaac agccaaacga ccatcagcca gactagccac tttattcagt
    19201 cttatatcaa aaaactagaa accacctcga ctggtttgac ccagcagacg gatgttctgg
    19261 cctatgctga gaatcccagt caagacaagg tcgagggaat ccgagatttg tttttgacca
    19321 tcttgaagtc agataaggac ttgaaaactg ttgtgctggt gaccaaatct ggtcaggtca
    19381 tttctacaga tgacagtgtg cagatgaaaa cttcctctga tatgatggct gaggattggt
    19441 accaaaaggc cattcatcag ggagctatgc ctgttttgac tccagctcgt aaatcagata
    19501 gtcagtgggt catttctgtc actcaagaac ttgttgatgc aaagggagcc aatcttggtg
    19561 tgcttcgttt ggatatttct tatgaaactc tggaagccta tctcaatcaa ctccagttgg
    19621 ggcagcaggg ctttgccttc attatcaatg aaaaccatga atttgtctac catcctcaac
    19681 acacagttta tagttcgtct agcaaaatgg aggctatgaa accctacatc gatacaggtc
    19741 agggttatac tcctggtcac aaatcctacg tcagtcaaga gaagattgca ggaactgatt
    19801 ggacggtgct tggcgtgtca tcattggaaa agttagacca ggttcggagt cagctcttgt
    19861 ggaccttgct tggggccagt gtcacatctc ttcttgtctg tctctgctta gtgtggttca
    19921 gtcttaaacg ctggattgct cctttgaagg atttgagaga aaccatgttg gaaattgctt
    19981 ctggtgctca aaatcttcgt gccaaggaag ttggtgccta tgaactgaga gaagtaactc
    20041 gccaatttaa tgctatgttg gatcagattg atcagttgat ggtagctatt cgtagccagg
    20101 aagaaacgac ccgtcagtac caacttcaag ccctttcgag ccagattaat ccacatttcc
    20161 tctataacac tttggacacc atcatctgga tggctgaatt tcatgatagt cagcgagtgg
    20221 tgcaggtgac caagtccttg gcaacctatt tccgcttggc gctcaatcaa ggcaaggact
    20281 tgatttgtct ctctgacgaa atcaatcatg tccgccagta tctctttatc cagaaacaac
    20341 gctatggaga taagctggaa tacgaaatta atgaaaatgt tgcctttgat aatttagtct
    20401 tacccaagct ggtcctacaa ccccttgtag aaaatgctct ttaccatggc attaaggaaa
    20461 aggaaggtca gggccatatt aaactttctg tccagaaaca ggattcggga ttggtcatcc
    20521 gtattgagga tgatggcgtt ggcttccaag atgctggtga tagtagtcaa agtcaactca
    20581 aacgtggggg agttggtctt caaaatgtcg atcaacggct caaacttcat tttggagcca
    20641 attaccagat gaagattgat tctagacccc aaaaaggaac gaaagttgaa atatatataa
    20701 atagaataga aactagctaa ctcccagtca attctgggag ttttatgcgt aattgggcaa
    20761 actttctagg ttgtctatct tgctaaaaac aagaaaaaac ggtatgatag aagagtgaca
    20821 acagaggtaa cagagatgaa cgagacacag attcaaagag aaacacgtca ggtagtggaa
    20881 gatgttttag aaaagaccaa tttgaagcag ggggcccttt ttgttttagg tctatcttct
    20941 agtgaggtgc taggtggtca gattgggaaa gaatccagtc aagagattgg tgagctgata
    21001 gtggagacta tcttgggtat tctaggtagt agaggtatcc atttagcagt tcaaggttgt
    21061 gagcatgtca atcgggctct cgttgttgaa cgtcaggtgg cagagcagtt tggtttggaa
    21121 attgtcagtg tccatcctac tcttcatgca ggaggttcgg ggcagttggc agcctttaag
    21181 tttatgcagg atccagttga ggttgaattt atcaaggctc atgctggatt ggatatcgga
    21241 gacactgcaa ttggcatgca tgtcaagcat gttcaggttc cgattcgccc tattttgaga
    21301 gagattggcc atgcccatgt aacggcactg gctagtcgtc caaaattaat cggaggtgcg
    21361 cgtgcgcact atccgcaaga tgctattagg aagtcttgag aatgaataaa ggagataaaa
    21421 gtgtttaaaa aagaccgttt ttcaattcgt aagattaagg gagttgtagg ctctgtattt
    21481 cttggaagcc ttttgatggc tccttctgta gtggacgcag ccacctatca ctatgtaaat
    21541 aaagagatta tttcacaaga agctaaagat ttaattcaga caggaaagcc tgacaggaat
    21601 gaagttgtat atggtttggt gtatcaaaaa gatcagttgc ctcaaacagg gacagaagca
    21661 tctgttttga cagcttttgg tttgctgact gttgggagct tgcttttaat ctacaagaga
    21721 aagaaaattg ctagcgtctt tctagttgga gctatgggat tggtagttct tcctagtgca
    21781 ggggctgtag acccagttgc gaccctagca ctggctagtc gagagggtgt tgttgaaatg
    21841 gagggctatc gctatgttgg ttatctatca ggtgacatcc tcaaaacgct tggcttggac
    21901 actgttttag aagaaacctc agcaaaacct ggagaggtga ctgtagtcga agttgagact
    21961 cctcaatcaa caacaaatca ggagcaagct aggacagaaa accaagtagt agaggcagag
    22021 gaagctccaa aagaagaagc acctaaaaca gaagaaagtc caaaggaaga accaaaatcg
    22081 gaggtaaaac ctactgacga cacccttcct aaagtagaag aggggaaaga agattcagca
    22141 gaaccagctc cagttgaaga agtaggtgga gaagttgagt caaaaccaga ggaaaaagta
    22201 gcagttaagc cagaaagtca accatcagac aaaccagctg aggaatcaaa agttgaacca
    22261 ccagtagaac aagcaaaagg cccagaacaa cccgtgcaac ctacacaagc tgagcaacca
    22321 aggataccaa aagattcatc acaaccagaa gatcctaaag aagatagggg agcggaagag
    22381 acaccgaaac aagaagatga acagccagca gaagccccag aaatcaaggt tgaagaacca
    22441 gtagaatcaa aagaggagac taaaacagct aaggggacgc aagaagaagg caaagaaggt
    22501 caagcaccag tacaagaagt aaacccagaa tataaagtaa caacaggtac agttgagaag
    22561 tctaccgaaa gtgaattaga tttcacaaca gaagtagttc cggatgacac aaaatatgta
    22621 gatgaagaag ttgttgaacg tcaagggtct aaaggagttc aagttacgaa aacaacttac
    22681 gaaacagtgg aagttgtaga gacagacaaa gtcctttcta caactacaga agtgaaaact
    22741 cctgctgttc ctaaagtcgt taaaaaagga acaaaaccag ttaaaggaac agcagtcgaa
    22801 acaagagaag aagtcattcc ttttgctacg aaagaacaag aagatgacac tttaaaacgc
    22861 ggaacaagac aagtagctca agaaggtgta aatggtaaga aacaaattac tgaaacctat
    22921 aaaacaattc gcggagaaaa aacaaacgaa gctccaacag tagaagaaac agttctccaa
    22981 gcacctcaag atgagattat caaaaaaggc acaaaaggtt tagaaaaacc aaccttagaa
    23041 tgggttaaaa cagataaaga tgtcttgaag aagagtgcga cagccagtta tacattgaat
    23101 aaaccagatg gggtagaaat caaatccatc aaagtagctc taaaagataa tactggaaca
    23161 gttattaaag aagtaacggt agaagaaaat aacttaaacg ctactttaga caacttgaaa
    23221 tattatcaag gttatacact atccacaacc atggtctata atcgaggcga aggcgaagaa
    23281 actgaaacgt tagaagataa agaagttcag ttagacctta aaaaagttga aatcaaaaac
    23341 atcaaagaaa ctagcttgat gagtgtggat gatgctggtg ttgaaacaga taagagctta
    23401 cttactgaaa aaccgacaga cgttgctcca ctttacctac gagtgacaac tcatgataac
    23461 aaaacaacaa gactggcagt aagttcagtt gaagaagttg ttgttgatgg aaaaacattg
    23521 tacaaagtgg tagctaaagc tcctgactta gttcaacgta gagaagatga tacactaagc
    23581 gaagagtatg tacattactt tgaaaaacaa ttaccaaaag tagataatgt gtactatagc
    23641 tttaacgagt taattacaga tatgcaaaaa aatcctactg gtacattcaa actaggtgca
    23701 gatcttaatg cagcgaatac tccaactcca agtaaatcat atgtaacagg tgaatttaaa
    23761 ggtaaattat caagtgttga tggtcaacat tatacgattc acaatactgc gcgcccatta
    23821 tttaacaata ttgttggtgg taccgttaaa aatattaatc ttaacaatgt aaatatcgat
    23881 atgccttggg ctgatcaaat tgcatctgta gcaaatgtga tcaaaggtgg tagccttatt
    23941 gagaatgtta aagtcaaagg taatgtgctc ggcagaaact gggtatctgg tttcattgat
    24001 aaaattgaca gtggtggtag attagttaat gttgcattta ttggtaatgt aacttctgtt
    24061 ggagatgggg ataaattctt aacaggtatt gttggagaaa actggaaagg atatgtcgaa
    24121 aaagcatacg ttgaagctaa tattaaaggt aagcgagcta aagctgctgg tatatcttat
    24181 tggtcgcaaa atagcggaga taataatgct gtcggtagag atggagcaat taagaaatct
    24241 gtcgttaaag gttcaataga tgtagaaaaa ccaattgaag ttggtggcgc tgttggaagt
    24301 ttaaattatc ttggttatat agaagacact gttgctatga tgaaagttaa gaacggtgaa
    24361 attttctacg gttcgcatga tatcgatacc gatccatatt acacaggaga acgagtaaat
    24421 agaaatttca ttgttgacgg tgtaagtgaa ggtaaatctt cgtatacata ctctaaacaa
    24481 caaaagcgta ttaaatcaat atctcaagag gaagcagata agaaaattaa agaattgggt
    24541 atcacagcag ataaatttac aattatcgat ccaattgtaa ataaacttaa caacatcaaa
    24601 caaaaagctg atacttataa agatactcaa gattacaaag cagaccgtga acttacttat
    24661 cgtaatatcg aaaaacttca accattctac aataaagagt ggattgtaga ccaaggtaat
    24721 aaagttccat caaattctaa attattaact acagaagtat tatctgttac aggtataaag
    24781 agcggtcaat tcgtaactga tttgtcagat attgataaaa ttatgattca ctattctgat
    24841 ggagcaaagg aagaattgaa tgttactcgt caagaatcaa acgttcaaca agtgcgtgag
    24901 tacagtatca cagatttaga tatactctat acaccaaata tggttgagaa agatcgcgct
    24961 cagttgatga ctgatgtaaa gtctaagcta tctagtgtag aattagagtc tgatggagtg
    25021 cgtcaacttc tcgttaagag agatactaag aaagatgcga atgcaaatag tgtaggtcgt
    25081 caaaatggat acattagaga tttattcctt gaagaaagtt tctctgaagt taaagcaaat
    25141 cttgacaaat tggtaaaaca gatacttgaa aatgaagatc atcaactcaa cgataacgaa
    25201 ttagctgaac gtgctttact gaagaaagtt gaagataata aagctaaaat catgatggga
    25261 ctggcatact taaatcaata ttatgctttt aaatacgatg agttatcaat taaagatatt
    25321 atgatgttta aacctgattt ttatggtaag acagcaagtg ttattgatag attgattaat
    25381 attggttctg cagaaaataa tctaaaagga gatagaacgc aagatgctta tcgtggtata
    25441 atttcagggg caacaggtaa aggtagttta catgacttct tgacctacaa tatgaaatta
    25501 ttcacaaatg aaacagacat aaatgtttgg tttaagaaag ctattgaaaa gaatgcttat
    25561 gtagttgaac aaccatcaac gaaccctgca tttgccaaca aaaaatatcg tctatacgaa
    25621 ggaattaata atgggcaaca tgggcgcatg attttacctc ttcttaactt gaaaaatgca
    25681 catctattta tgatttcaac atacaacact atctcgttta gttcatttga aaaatatggc
    25741 aaagacacag atgaaaaacg tgaaaagttc aagagtgaga tcaataaacg tgctaaagag
    25801 caggttaatt acttagattt ttggtcacgt cttgctactg ataatgtccg tgataaactt
    25861 cttaagagtc aaaatgtagt tccaactcct gtatgggata atcataatag cccgaacgga
    25921 tgggctagta gacatggtca tatagatggg aaaccggatt atgctccaat tcgtgagttc
    25981 tttgggcgaa tcaataagta tcatggttac aaatatggat atggcgcata tgcatatatt
    26041 tttgcagcac ctcaaccgat ggatgcagtt tatttcgtta tgacagattt aattagtgac
    26101 tttggaacat ctgcatttac acatgaaact actcacgtta atgatcgtat ggcttactat
    26161 ggtggtcatt ggcatcgtga aggtacggat cttgaagcat ttgctcaggg gatgttacaa
    26221 acgccatcgg tatcaaatcc aaacggagaa tatggcgcat taggtctaaa tatggcgtat
    26281 gaacgtcaaa atgatggaaa tcaatggtat aacacaaatc caaacgacct tacaactcgt
    26341 gcagaaattg ataactacat gaaaggtttc aacgatacat taatgcttct tgattacctt
    26401 gaaggagaag ctgtcctcaa taaagcaaat caagatttaa acaacgcttg gttcaaaaaa
    26461 gtggataagc aacttcgtgg agctagtacg aagaaccagt atgataaagt tcgtgattta
    26521 acggcagatg agaaaaatat cactctaaat tcagtagatg atttagttga taataatttt
    26581 atgacgaaac acggacctgg aaacaatgta tatgatccaa ctgggttcgg aacggcctac
    26641 gtgactgtac ctattactgc aggtatttat ggaggtaaca caagtgaagg tgctcctggt
    26701 tctatgtcat tcaaacataa cacattcaga atgtgggggt actacggata tgaaaaagga
    26761 ttcttaaact atgcttctaa catgctgaaa aatgagtcta gacaggctgg tcacaatact
    26821 ctaggtgatg atttcatcat taagaaagtt tctgataata aattcagtac attggaagat
    26881 tggaagaaag catacttcaa agaagttgta gataaagcta aagcaggatt taaccctgtt
    26941 acaatcgacg gtactacata tagtagttac gatgacttga agaacgcatt tgctgcagca
    27001 gttgaaaaag ataaagctac tcttaaaaat ggatcagtta aatctgacaa tactgttgca
    27061 cttaaagaga aaatatacaa aaaacttctt caacaaacag atagctttaa aacttcaatc
    27121 tttagataat cgaaatctct catctgcttt gcggatgagg gttttcttat tttatgctat
    27181 acttaaggta tgcatagaaa aacagtgatt gattttagga ctttagggga gagatatacc
    27241 tttacccagc ctattaaaga gttgaaaacg agaaatgtag aagaagtggc agatttgctg
    27301 gcacaagtgg aaagctacca agagcaaggt tattatgtgg tggggtatgt cagctacgag
    27361 gctgcacctg cttttgagga gaaattaaca gttcacaagg ttcctctact gggcgagtac
    27421 ttgctttact ttactgttca cgatagggta gagacctccc ctattcctct gacttatgag
    27481 gatattgatt tgccctcaaa ttggcaggaa gtgacgtctg cagcagacta tgaaaaggcc
    27541 attgcccaga tacaccatca cttgaggcag ggagacacct atcaggtcaa ctacaccgtc
    27601 caactcaagc aaaagttaag tgccaatcct tttgctatct acaatcgtat ggtggtagag
    27661 caggaggcgg gctacaatgc ctacgtggaa catgacgaga tggcagtgat ttccatgagc
    27721 ccagagctct tttttgagca aaatgatcgc gagttgacaa cacgaccaat gaaggggacg
    27781 actcagcgtg gggtaactga ccaagaagat cttgaacagg ccagttggtt ggaacaggat
    27841 cccaaaaatc gctctgaaaa tatgatgatt gtggacctct tgcgcaatga tatgaaccgt
    27901 atttctgaag ttgggagcga gcacgtggag cgtctgtgtc aggtagagca gtattcaact
    27961 gtttggcaga tgacttcgac catcaagagt cagttgcgag aggatgtgga ccttgttgaa
    28021 atcttccgct cactctttcc ttgtggttcc ataacgggtg caccgaaaat tgcgacaatg
    28081 gagattatca aggacttgga gcctcaaccg cgtggagtct actgtggaac gattggtctc
    28141 ttgcttccaa atggacgacg gatttttaat gtggccattc gtaccattca acttcacaaa
    28201 gatcaagcca tttatggagt tggcggaggg attacttggg atagcacatg ggaatctgaa
    28261 taccgagagg ttcatcaaaa ggcagcagtt ctttatcgta aacaagctcg cttccaactg
    28321 attacgacag ggaaaatcag ccagaagaac ctgctgtttg aagatcaaca tctggaaaga
    28381 ctgagaaaag ccagtcgtta ttttgccttt ccttttgatg cagaagactt gggacacaag
    28441 attgaggaag agtgtcagga ttgtgaagct aatcgagatt accgcttgcg aatcagcctt
    28501 agcaaatctg gagagataga agtcaatcgt caagtattaa cccctctcag tacaagcttt
    28561 tgtcaggccc aagtctgtct tcaggaagct gctttgaatc aatcctttac ctactttaaa
    28621 accactcacc gaccgcattt gagcctagga gaacaagaga agatttacca caataagtca
    28681 ggagaactgc ttgaaacctc tataggaaat ttggttctga aaatcgctgg aaaactctac
    28741 acaccgccta tccgacttgg aatcttgcca ggaatttacc gtcagcattt gctagaaaca
    28801 ggacaggtag aagagaaagt cttgaccttg gcagatttag cccaagcaga agctatttac
    28861 ggctgtaatg cagtgagagg cttgtatgaa ctaagccttg aggagaacta gatgaaattg
    28921 atatttttgc atgggttagg gcagtcagca gagtcttgga aagaagttcg gaatctactg
    28981 acagattatc cttctgaggc tatcgagtta tttccttctg gagttagtaa ctatcaacaa
    29041 gctaaagagc gagtttatca gcacctagct caagagacag aaccttttgt tttgattgga
    29101 ttgtccttag gtgctgccct gacactagaa ctttccagtt acgatttacc aaatcttcga
    29161 gctttgattc tgtcaggatg cccgcttaaa ttagcaggga atatcctatt ttatcttcaa
    29221 ttgctgattt ttaaactact tcccaaaaga gtatttgaaa aacagggagc agacaagact
    29281 cttatggtcg gagtttctga ggaattgaag acacttgatt taacggatat agcagggact
    29341 tgctcctatc caaccttact aatttgtggc agcaaagata aaccgaatct tagttctatg
    29401 aaagctctcc atagactgtt aacagattcg cagtttcaga tcattcctga tggccctcat
    29461 gtcttgaata aggcaaaacc aaaggagttt gtagaaaaga ccagaagttt ccttgaattg
    29521 ctgaaataag tttgataaaa taatattgaa atcgatatca taattatggg agaaagaaat
    29581 gaataaacgt ctatttttaa aaatgagtct ggtgacgttg ccaattttag ccttgttttc
    29641 acaaccagtt ttggcggaag aaaacatcca tttttcgagc tgtaaggaag cttgggcgaa
    29701 tggctattcg gatattcacg agggagaacc tggttattct gccaagttag accgtgatca
    29761 tgatggtgtg gcttgcgaat tgaaaaatgc tcctaagggt gcttttaaag caaaacaagc
    29821 ggctacaact caaactgata caacttcatc cacagcaagt ggttgggtta agcaggacgg
    29881 ttcttggtac tattttgatg gaaatggaaa tctagtgaaa aatgcatggc agggaaacta
    29941 ttacctgaaa gctgatggta aaatggcaca gagtgaatgg atttatgact cttcttatca
    30001 agcttggtat tacttgaaat cagatggttc atatgctcgc aatgcatggc aaggaaacta
    30061 ctatttgaaa tcagatggta aaatggctaa aggtgaatgg gtttatgatg ccacctatca
    30121 agcttggtat tacttgaaat cagatggttc atatgctcgc aatgcatggc aaggaaacta
    30181 ctatttgaaa tcagatggta aaatggctaa aggtgaatgg gtttatgatg ccacctatca
    30241 agcttggtat tatttgacat cagatggttc ttatgcttac agtacatggc aaggaaatta
    30301 ctatctaaaa tcggatggta aaatggctgt caatgaatgg gttgatggtg gacgttatta
    30361 tgttggcgct gacggagttt ggaaggaagg tcaagcaagt acagcttctt ctagtaatga
    30421 tagcaatagt gaatattctg ctgctttagg aaaggcaaag acttataact cactattcca
    30481 tatgtcaaaa aaacgcatgt atagacaatt aacttctgat tttgataaat tttcaaatga
    30541 tgcagctcaa tatgccattg atcatttaga tgattaacag atactggtag gcgaaaaaat
    30601 tccttataat tcatttacgc aaacctttgc ataagtgaat ggattttgat atactagtac
    30661 tgtaagcatt ttcaattact acacaatgaa aaaggagaat atgatgagtc aaaagattat
    30721 tgggattgac cttggcggaa cttctatcaa atttgcaatc ttaacaacag caggagaaat
    30781 ccaaggaaaa tggtcaatca agaccaacat tttggatgag ggaagtcata tcgttgatga
    30841 tatgattgag tctattcaac atcgtttgga cttgcttgga ttggcagcag cggacttcca
    30901 aggcattgga atgggatcac caggtgtggt tgaccgtgac aaagggactg ttatcggtgc
    30961 ctacaacttg aactggaaaa cccttcaacc aatcaaacaa aagattgaaa aagctttggg
    31021 cattccattt ttcatcgata atgatgccaa cgtagcagct cttggtgagc gctggatggg
    31081 tgctggtgat aaccaaccag atgttgtctt tatgacactc ggtactggtg ttggtggtgg
    31141 tattgtcgca gaaggcaaat tgcttcatgg tgttgctggt gcagcaggtg aacttggtca
    31201 tatcactgtt gattttgacc aaccaattgc atgtacttgt ggtaaaaaag gctgtcttga
    31261 gacagtcgct tctgcaacag gaattgtcaa tttgactcgt cgctatgccg atgaatacga
    31321 aggcgatgca gccttgaaac gcttgattga taacggagaa gaagtaactg ctaagactgt
    31381 ctttgatctc gcaaaagaag gagacgacct tgctttgatt gtttaccgta acttctcacg
    31441 ttacttggga atcgcttgtg ctaacattgg ttcaatccta aacccatcaa caatcgtcat
    31501 cggtggtggt gtgtcagctg cgggagaatt ccttctacaa ggtgttcaaa aagtttacga
    31561 tgaaaatagt ttcccacaag tacgcacatc tactaaatta gctcttgcaa ctctaggaaa
    31621 tgacgctgga gttatcggag cagcatcact tgtattgcaa taagataatg agagaggaaa
    31681 atgctaacct agagttagta atgttcctct tttatttttt atgctatact agttaggaca
    31741 atgaatgagg tgagagtaaa tgacaaaagc agatacgatt tttaaagaga atattgaacg
    31801 cattctaaag gaaggtgttt tctcagagca ggctcgtcct aagtacaagg atgggactgt
    31861 tgctaattcc aagtatgtaa ctggtgcctt ttccgagtac gacttatcta aaggagaatt
    31921 tcctatcaca accttgcgtc ccattgcaat caaatccgcc atcaaagaag ttctctggat
    31981 ttaccaagac cagtcaaata gcttagaagt gcttaatgac aagtacaatg ttcactactg
    32041 gaatgactgg gaagttggag acacgggaac cattggtgag cgctatggtg ccgttgttaa
    32101 gaaacacgac attatcaata agcttctcaa acagttggaa accaatcctt ggaaccgccg
    32161 caatattatt tctctctggg attaccaagc tttcgaagaa acagatgggc tgctcccgtg
    32221 cgcctttcag accatgtttg atgttcggcg tgttgatggg gaaatctatc tggatgcgac
    32281 cttgacccag cgctccaatg atatgctggt ggcccaccac atcaacgcta tgcagtatgt
    32341 ggctttgcag atgatgattg ccaaacattt tggctggaag gttgggaagt tcttctactt
    32401 catcaacaac ctccatatct atgataatca atttgaacaa gctcaggaat tgctccgtcg
    32461 ggagccgtca aactgccaac cacgcttggt tttaaatgtt cctgatggga ctaatttctt
    32521 tgatatcaaa gcagaagatt ttgagttggt ggattatgac cctgttaagc cacagttgaa
    32581 gtttgaccta gctatttaaa agaatagaaa aagaagttga gaataatccc aacttctttt
    32641 gtttcttaac gtgatacgcg gcgacgagct gcttttttac ggttttcttc gatgaaagct
    32701 gctttttgct cttctggttc gattactttc tttttaaatg cgtatactgc acctgcaacg
    32761 gcagcgacag ttcctgcgac acctgttaca agacctttag cgaatccttt agccatgagt
    32821 cttcctcctt tatattctca atcagccagc ctcctcaaga ggtcacattt ttctgactga
    32881 cctttttgtg ttataataat agtaacgaaa aaatgggaat ttttcaagga aaaaagatga
    32941 aaacaaaaat aattgtgatt gttggaccga ctgctgttgg aaagacggct ctagctattg
    33001 aagttgcaaa gcgttttaat ggcgaagtgg ttagtggaga tagccaacaa gtctatcgag
    33061 gacttgatat tgggacggct aaggctagtc cagaagagca ggcagctgtt cttcatcatt
    33121 taatcgatgt tagagagata accgagtctt actcggcttt tgattttgtt tcagaagcta
    33181 agatgactat tgaggatatt cacagccgtg gcaagctagc cattatcgcc ggtgggactg
    33241 gactttatat ccagagcttg ctagaaggtt accacctagg tggggagact cctcatgagg
    33301 agattttagc ttatcgagct agtttggagc catattcaga tgaggaatta gcccatttgg
    33361 tggagcaagc aggccttgag attccccagt ttaatcgtcg tcgtgctatg cgtgccttag
    33421 aaattgccca ttttggtcag gatttggaaa atcaagagac cttgtatgaa ccgctgatta
    33481 tctgcttgga tgatgaacgt agtcaacttt atgagcgtat caaccaccga gtggatttga
    33541 tgtttgaggc tgggcttttg gatgaggcca agtggctgtt tgaccattcc cctaatgtac
    33601 aggctgctaa aggaattggc tataaggaac tctttcctta tttccgtgga gagcagacct
    33661 ttgaggaagc tagtgagagt cttaaacagg cgacccgtcg ttttgccaag cgtcagctga
    33721 cctggttccg taatcgcatg caggtcacct tttatcagat tggagaatct ggtgtacaag
    33781 accgcatttt aagccagata gaggagtttt tagatgattg aaacggagaa aaaagaggag
    33841 cgagtcctgc tgattggtgt ggaattgcag ggtatggaca gttttgacct ctccatggaa
    33901 gaattggcta gtttagcgaa aacggcaggg gcagtcgttg tagatagcta cagacaaaaa
    33961 cgtgaaaaat atgattccaa gaccttcgtc ggctctggta agttggaaga gattgcgctt
    34021 atggtggatg cagaagaaat cactactgtc atcgtcaaca atcgtctgac cccaaggcag
    34081 aatgtcaatc tagaggaagt tctcggtgtt aaggtcattg accgtatgca gttgattttg
    34141 gatatctttg ccatgcgggc tcgaagccat gaagggaagc tccaagtcca cctagcccaa
    34201 ctcaaatacc ttttgcctcg cttggttggt caggggatta tgctcagccg tcaggcaggg
    34261 ggaattggtt cccgtggtcc tggtgaaagc cagctggagc tgaaccgtcg tagcgttcgc
    34321 aatcaaatca cggatatcga gcgccagctc aaggtggttg agaaaaatcg ggcgactgtc
    34381 agagaaaaac gtttggagtc tagcaccttt aagattggtt tgattggtta taccaatgct
    34441 gggaaatcaa ctatcatgaa catcttgacc agtaagaccc agtatgaagc agatgagctc
    34501 tttgcgactc tggatgcgac aaccaagagt attcatctgg gaggcaatct ccaagtaact
    34561 ttgacagata ccgttggctt tattcaagat ttgccgacag agttggtgtc cagtttcaag
    34621 tcaaccttgg aagaaagcaa gcatgtggac cttctggttc atgttatcga tgctagcaat
    34681 ccttaccacg aggagcatga aaaaacggtt ctctccatca tgaaagacct ggacatggaa
    34741 gatattcctc acttgacgct ttataataaa gcggatttgg tggaggattt cacgcctacc
    34801 caaacgccat ataccctcat ttctgccaag tctgaggaca gtcgtgaaaa cttgcaagca
    34861 ttattgctag ataagattaa ggaaattttt gaagcattta ccctgcgagt gcctttttca
    34921 aagtcctaca agattcatga tttagagagt gttgcaattc tggaagaacg tgattatcag
    34981 gaagacggcg aagtgattac aggctacatt tccgagaaaa ataaatggag gttagaagaa
    35041 ttttatgact gatattaaaa ctttggctct aaagtatgga ggttatacaa gtctggacaa
    35101 ggtctatctg gatcagcttc tagctggtaa aacagagcag gagcagttaa cacttattac
    35161 acctccgcca agtgttgtta atgcctactt tgcagaactc tatcagaaaa aaagtcctca
    35221 ggctgcgacg gattattttg cagaactcag tcaggaactg aatctttaca atactgaacc
    35281 aagtttcacc ctagaaaata agccttttat tcggcttaat ctgtctggta aatcctttgg
    35341 tttttgttat gagagtgacg gctttggtcg gattttctct gaaaatgagg agaaaatctc
    35401 ggatgacttg ctctttgaaa ttgcgcaaat tttctcccat caattagtct ttgaagagtc
    35461 tggtaagatt tacatgaagc ctgtcaagga cgaggaagtt gtcagcctag aaagtctcac
    35521 agctttgact gatttggaaa gattggctga tggtcgaaaa cgtctcaaag gctacagcca
    35581 agaggattta ttgcaagaag ctgctgcttt ttctggcaaa cgctatttcc gatcggaaaa
    35641 ccgcacagcc atgttatata ttgattaatt agaaagtatc gaatggatat tcaattttta
    35701 ggaacggggg ctggtcagcc ctctaaagcc cgcaacgttt caagtctcgc cctgaaactt
    35761 ttggacgaga ttaacgaagt ttggctcttt gactgtggag aaggtacgca aaatcgcatt
    35821 ctggaaacca caattcgacc acgtaaggtc agcaaaatct ttattaccca tctgcatgga
    35881 gaccacattt ttggtttgcc aggtttcctt tctagccgtg cctttcaggc caatgaagag
    35941 cagacagatt tggaaatcta cggacctcaa ggaatcaagt catttgtctt aaccagcctt
    36001 cgtgtgtcag gttctcgtct gccctaccgc attcatttcc atgagtttga ccaagattct
    36061 ctgggtaaaa ttcttgaaac cgataaattc actgtgtatg cagaggagct ggaccacact
    36121 attttctgtg ttggctatcg tgtcatgcaa aaggatctag aagggacgct ggatgctgaa
    36181 aaactcaagg ctgctggtgt tccgttcggc ccgctttttg gtaaaatcaa aaacggccag
    36241 gatcttgttt tggaagacgg aactgaaatc aaggcagcag actatatctc agcgccacgt
    36301 ccaggtaaga ttatcactat tttaggagac actcgaaaaa cggatgccag tgtgcgtctg
    36361 gctgtcaatg cagatgtcct agttcatgag tccacttatg gcaagggtga tgaaaaaatt
    36421 gctcgtaacc atggtcactc aactaatatg caagctgcac aagtagcggt agaagcaggt
    36481 gccaaacgcc tcctactcaa ccatatcagt gcccgtttcc tctcaaaaga tataagcaaa
    36541 ctcaagaagg acgctgccac aatttttgaa aatgtccatg tggtcaaaga cttggaagaa
    36601 gtggaaatct agcagtcaca gaaaggataa gtatgcctac tattctcatt accggagcta
    36661 gcggtggtct agctcaagaa atggtcaaac tcctgcccaa tgaccaactc atcttgcttg
    36721 gtagaaataa ggaaaaatta gcccaactct acggaaatta ttcccatgca gaattgattg
    36781 aaattgatat taccgacgat tcagccctag aagctctggt aactgatctt tatctccgct
    36841 atggcaagat tgatgtcttg attaacaacg ctggttacgg gatttttgag ggatttgacc
    36901 agattgctga taaagatatt caccagatgt ttgaggtcaa tacctttgcc ctgatgaatc
    36961 tgtctcgtca ccttgcggct cgtatgaagg aaagcagcaa agggcatatc atcaacatcg
    37021 tcagcatggc aggtctaata gctactggca agtctagtct ttactcagcg accaagtttg
    37081 cggctattgg tttttcaaat gctctgcgac tcgaacttat gccctatgga gtctatgtga
    37141 caacagtcaa tccaggtcca atccgaacag gattttttga ccaagctgac ccagatggaa
    37201 cttatcttaa atcggttgac cgcttcctct tagaggcaga tgcagtggct aaaaagattg
    37261 tcaagattat aggaaaaaat aaacgagaac tcaatctccc gattttgttg aacctagccc
    37321 ataagtttta tactctcttt cccaagctag ctgataagtt ggcaggggaa acttttaatt
    37381 ataagtaaaa agaaccaatg tgcaggttgt tgctagccta catattggtt cttatcattt
    37441 ctcaactatc aaactgaact tcttctagta gatagtcgat aaagcgttcg cccatcttag
    37501 ataggctggt tttctcatgc tggatataga ccagctcaat cgggtcatca atatccagtg
    37561 ggatagaaac gatattgtct ccgtttaggt tgctgttcaa aatccctgtc gcaatggtat
    37621 aaccatccaa accaatcaag agattaaaga gggtggcacg gtcactgacc acaatggatt
    37681 tcttgtggtg ttcttgagaa agaatctctt ctgaaaagta gaaggagttg tgcgtccctt
    37741 ggtcatagct gaggtaaggg aaattctcca aatcagacag tttcaccttg tctttctttg
    37801 ccagagggtt ggtcttgctg acaaagatat gcggttgcgc tgtgaagaga tggtgggcta
    37861 gtaggtgatt gtcatccagc atcttggtta aaacatcacg gttgtaactg tttaagaaga
    37921 ggaccccgac ctcactgcgg aagttcttga cgtcgtcgat aatctcccaa gtccgagttt
    37981 cacgaaggaa aagctcgtat ttctccatat cgcttttctt gagcaaagag acaaaggcat
    38041 tgaccacaaa ggcatagtgt tgagacgaaa cgctaaagag ttcgcggtgg gcgacaggat
    38101 ttttatagcg ttcctccaga agctgagttt gctcgacaac ctgacgggca taagagagaa
    38161 actccatccc atcacgggtc aaggtgattc ccttgggatt gcggataaag atctcaatgc
    38221 ccatttcatt ttccaaatct ctcacagcat tggaaagact tggctgagtg ataaagagtt
    38281 gcttggctgc ctcattcata gagccagttt cgacgatttt gataatataa tgtaattgtt
    38341 gaattctcat gtttttattg taccacactt gccttagaat cgccaaagaa gataggaaaa
    38401 taaggagcat tgtgtgagtc tcttcttgaa aaaaacacaa aattttgcta gaatgagtct
    38461 tatgaaaaca ttctatgatg tgcagcaatt cctcaaacga tttggtatta ttgtttacat
    38521 gggaaaacgc ttatatgata ttgaactgat gaagttggaa ctatctcgga tttacgatgc
    38581 agggttgatg gataaattag actatctaga ggcagaagca gttcttcgca gagagcacaa
    38641 ggtagaattg gattatattg agaaaaatgg agaaaagaac taatggttac ttggattttg
    38701 tgggcactta tactagcaat gttggcgtgg atgggcttta actatcttcg tattcgtcgt
    38761 gcggctaaaa ttgtggacaa tgaggagttt gaagccttga ttcgtacggg tcaattgatt
    38821 gatttgcgcg acccagcaga attccacaga aaacatatcc ttggtgcacg caatattcct
    38881 tcaagtcagt tgaaaactag tcttgcagcc cttcgtaaag ataaacctgt ccttctctac
    38941 gagaaccaac gtgcgcaacg agttacaaat gcagctcttt acttgaaaaa acaaggtttt
    39001 tctgagattt atatcctttc ttatggcttg gattcttgga aagggaaagt gaagactagc
    39061 taaccttatt tccaaattag tcgaatgata ggatgaagct attgttttat agtataatta
    39121 atttgtataa aatttaaaga gttcttagaa catgaaagaa aagaagaaaa atccactttt
    39181 tgtgggtatc ttgtcgatta ttcttggttt gttatttcca attgtaggct tgattttggg
    39241 tatcatagga ttggtcttgg ctatttcata ccaaaaagag tcacaattag actataaaat
    39301 agaaaagatt cttaatatct
//
LOCUS       DAVHIU010000005        36629 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVHIU010000005 DAVHIU010000000
VERSION     DAVHIU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.005"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(365..1213)
                     /locus_tag="VVN06_000208"
     CDS             complement(365..1213)
                     /locus_tag="VVN06_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5561824.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQSASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1365..1643
                     /locus_tag="VVN06_000209"
     CDS             1365..1643
                     /locus_tag="VVN06_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561825.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1645..3708
                     /locus_tag="VVN06_000210"
     CDS             1645..3708
                     /locus_tag="VVN06_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5561826.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3786..5042)
                     /locus_tag="VVN06_000211"
     CDS             complement(3786..5042)
                     /locus_tag="VVN06_000211"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5561827.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5184..7649
                     /locus_tag="VVN06_000212"
     CDS             5184..7649
                     /locus_tag="VVN06_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU5561828.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEVTQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7714..8625
                     /locus_tag="VVN06_000213"
     CDS             7714..8625
                     /locus_tag="VVN06_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5561829.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8855..9553
                     /gene="dapD"
                     /locus_tag="VVN06_000214"
     CDS             8855..9553
                     /gene="dapD"
                     /locus_tag="VVN06_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU5561830.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVKI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9621..10751
                     /locus_tag="VVN06_000215"
     CDS             9621..10751
                     /locus_tag="VVN06_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU5561831.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10763..11302
                     /locus_tag="VVN06_000216"
     CDS             10763..11302
                     /locus_tag="VVN06_000216"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU5561832.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11286..11963
                     /locus_tag="VVN06_000217"
     CDS             11286..11963
                     /locus_tag="VVN06_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5561833.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12035..12934)
                     /gene="galU"
                     /locus_tag="VVN06_000218"
     CDS             complement(12035..12934)
                     /gene="galU"
                     /locus_tag="VVN06_000218"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU5561834.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12956..13972)
                     /locus_tag="VVN06_000219"
     CDS             complement(12956..13972)
                     /locus_tag="VVN06_000219"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5561835.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14184..14564
                     /locus_tag="VVN06_000220"
     CDS             14184..14564
                     /locus_tag="VVN06_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5561836.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14738..15163)
                     /locus_tag="VVN06_000221"
     CDS             complement(14738..15163)
                     /locus_tag="VVN06_000221"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5561837.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15231..15881)
                     /gene="phoU"
                     /locus_tag="VVN06_000222"
     CDS             complement(15231..15881)
                     /gene="phoU"
                     /locus_tag="VVN06_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5561838.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15896..16648)
                     /gene="pstB"
                     /locus_tag="VVN06_000223"
     CDS             complement(15896..16648)
                     /gene="pstB"
                     /locus_tag="VVN06_000223"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5561839.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16650..17465)
                     /gene="pstA"
                     /locus_tag="VVN06_000224"
     CDS             complement(16650..17465)
                     /gene="pstA"
                     /locus_tag="VVN06_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5561840.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17458..18321)
                     /gene="pstC"
                     /locus_tag="VVN06_000225"
     CDS             complement(17458..18321)
                     /gene="pstC"
                     /locus_tag="VVN06_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5561841.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18439..19314)
                     /locus_tag="VVN06_000226"
     CDS             complement(18439..19314)
                     /locus_tag="VVN06_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU5561842.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19481..20812)
                     /locus_tag="VVN06_000227"
     CDS             complement(19481..20812)
                     /locus_tag="VVN06_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU5561843.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20813..21520)
                     /locus_tag="VVN06_000228"
     CDS             complement(20813..21520)
                     /locus_tag="VVN06_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5561844.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21604..22089)
                     /locus_tag="VVN06_000229"
     CDS             complement(21604..22089)
                     /locus_tag="VVN06_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5561845.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            22075..22632
                     /locus_tag="VVN06_000230"
                     /pseudo
     CDS             22075..22632
                     /locus_tag="VVN06_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22947..24638)
                     /locus_tag="VVN06_000231"
     CDS             complement(22947..24638)
                     /locus_tag="VVN06_000231"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5561846.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            24774..25220
                     /gene="argR"
                     /locus_tag="VVN06_000232"
     CDS             24774..25220
                     /gene="argR"
                     /locus_tag="VVN06_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5561847.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDKWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25271..27805
                     /gene="mutS"
                     /locus_tag="VVN06_000233"
     CDS             25271..27805
                     /gene="mutS"
                     /locus_tag="VVN06_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5561848.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEHISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            27952..29646
                     /gene="patA"
                     /locus_tag="VVN06_000234"
     CDS             27952..29646
                     /gene="patA"
                     /locus_tag="VVN06_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU5561849.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGIMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKVVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(29977..30246)
                     /locus_tag="VVN06_000235"
                     /pseudo
     CDS             complement(29977..30246)
                     /locus_tag="VVN06_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30438..32204
                     /gene="patB"
                     /locus_tag="VVN06_000236"
     CDS             30438..32204
                     /gene="patB"
                     /locus_tag="VVN06_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU5561850.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDISISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32343..33032
                     /locus_tag="VVN06_000237"
     CDS             32343..33032
                     /locus_tag="VVN06_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5561851.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            33036..33215
                     /locus_tag="VVN06_000238"
     CDS             33036..33215
                     /locus_tag="VVN06_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561852.1"
                     /translation="MEYLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            33202..34551
                     /locus_tag="VVN06_000239"
     CDS             33202..34551
                     /locus_tag="VVN06_000239"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5561853.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34675..36135
                     /locus_tag="VVN06_000240"
     CDS             34675..36135
                     /locus_tag="VVN06_000240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5561854.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            36149..36583
                     /locus_tag="VVN06_000241"
     CDS             36149..36583
                     /locus_tag="VVN06_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5561855.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        10692 a         7026 c         8058 g        10853 t
ORIGIN      
        1 aagaactgat ttcaactatt tccccttaat gtgtatggaa actataacaa aacgcataag
       61 attaaggttt gtgtacctgt tcttatacgt ttttgtcata ttgaagattt tcggtaagtt
      121 ttctactttt gcgaagtcta gtttatactc aatgaaaatt aaagagtaaa ctagccgtag
      181 gttgctcaaa gtacagcttt gaggttgcag ataaaactga cgaagtcagc tcaaaacatg
      241 gttttgaggt tgtagatgaa actgacgaag tcagctcaaa acatggtttt gaggttgtag
      301 atgaaactga cgaagtcagt aaccatacgt acggcaaggc gacgttgacg tggtttgaag
      361 agtattagaa tgctttccca accaaaatct ctgcttcgat ggtaatctct gtcagttggc
      421 tccagtcaat cttgctctgg tcaacgtgaa agaggagagg agtcatactg agtagggctt
      481 ggagttgttc tgctgtgata gtcttagtca gagaggcaga ttgactagat aggatggtaa
      541 agtgttcctg gaaatgctcc ttgatatctt ggttagaata ctccttgttt gtcagctggt
      601 cctgtactct ttgacggatt tctttgaggt gattttcagt tgggataacc tttatcaaga
      661 taccatcttt ggataaaacg cgacgaaatt ctccatagtt ggcaggtgag aagatatcaa
      721 gcaggatatc catgttagcg tcttttatag gaaggcgtgc caagtcgcca acgaaccaat
      781 tgactgccca gttgggttca cttttagcag cgatttggac tgaatctttg gagatgtcaa
      841 aggcatagaa agttttttca gagtgacttt cttgtagttt gcgagaatag aatccttcac
      901 cacaaccgat atccaaaatt gttgtggtag tttttgagct tgcaagcaag tcagatacag
      961 catctaagat agcttggtaa aagccggctt ctaggatttg ttgacggttt tgaaaatttt
     1021 ccttgtcata gttagcagat tgcttgattt gaggtgctag attgacatag ccaaatttcg
     1081 ccaagtcaaa agaatgacgg ttgcagcact tgaaattagt ctctaacaga gtcagatttt
     1141 cttgacagat aggacaggca aaggcagtcg cagaagcaaa acgctgaagt ttgggcttga
     1201 gatttgtatt catagtttaa gtgtatcaaa agaggcaaat gaaagcaact ttaggaataa
     1261 gtagatggga gattcagtag aaataaaact aattctccaa tttatagaat gaaattgctt
     1321 ttatatctaa attgctatat aataatgata aggaggaaat aagtatgtta aaagaagtat
     1381 taaccgttgc aaaagttgcg aaaaaatctt cattattttt gggtggtgtc gcatttggta
     1441 cccttggttt gaaaatctta gcaagtaagg aagctaaaaa aggttattct aaagctttgg
     1501 ctaaggctta caagttgaaa gacgagctag atgcatctgt ttctgttgtg aagcaacatg
     1561 gagacgatgt cttgcaagat gccaaatatt tgtacgagca agagaaaaaa gaagagcaat
     1621 tagatagcct tataggtgaa taatatgtct tttaaagtgc tacatagagg ataccaacat
     1681 atccgactat catcttcttt ttcacttacc ttggatattc aagactatct tcgttccttg
     1741 gcgagagatg aaaaggggat tgagtctatc cagttttaca tggatcaaca gcactttact
     1801 ctacgcataa aagaaggctt ttctgtatta gataatgcag aagccttttt aaaaagaatt
     1861 gataaaggga aagtttctga gttgatgact cttcccattc gtagagaaga gagtgcttat
     1921 tctattgttt caggtgcagc ggttaagcgt gtactttttc gtagttttgt gccgtatcct
     1981 attcgctata tatggacttg ttatcaggct ttgggttata ttagagaagc ctatcaaaca
     2041 ctagcgcgta aggaactaac gatggaagtc ttggactgtt cggctatttt attgtctttg
     2101 tttatgaacc aatccaagac agctagcaat atcatgttta tgcttgattt ggggaatcat
     2161 ttagatcagt ggtccttgaa aaaaactgca acagatttag aacagagtct tcttgcaaaa
     2221 gagagcgatg tattcctagt acagggcgat acggttgtta gtatcaagag ttccgatgtt
     2281 caaataggag atgtcttgat cttatctcaa ggaaatgaaa ttctgtttga tggacaagta
     2341 gtttcaggtt taggtatggt caacgaaagt tccttgacag gagagagttt tccagttgaa
     2401 aaaagagagt ctgatttggt ttgtgcaaat acagtattag aaactggaga gttacgcatt
     2461 cgtgtaacag ataatcagat gaacagccgt attttacagc tgattaagtt gatgaagaaa
     2521 tctgaagaaa acaagaaaac gaaacaacgc tatttcatca agatggcgga taaagtcgtc
     2581 aaatataatt tctttggggc tgggctgact tacctattga caggttcttt ttctaaagct
     2641 atttctttcc tattggtcga tttctcctgc gctttgaaaa tctctactcc tgtagcttat
     2701 ttgacagtta tcaaggaagg gttgaaccgt gaaatggtga ttaaggatgg agatgttctg
     2761 gagaaatatc tggaagttga tactttcttg tttgataaga caggaacaat cacaactagt
     2821 tatcctatag ttgaaaaggt gttacctttt ggagactata gtgaggaaga tattctcaga
     2881 atcagtgcct gtcttgagga acacatttat catcctattg ctaatgccat cgtcaagcaa
     2941 gctgagatag agggaattga acatgaggaa atgcatggga aactccaata tatcgcaagc
     3001 aaggggatca aatctcatat agatggccaa ccagttctta ttgggaatta tgtcttgatg
     3061 caggatgagc agattcatat cagttcagaa caaaatgctt taattgaaga gtacaagagt
     3121 cactacaatc tcttattctt ggcttatcaa aatgaattga ttggaatgtt ctgcattcat
     3181 actcctttga gaaaagaagc aaaaacagcc ttggataaac ttaaggcaca ggggaaaaaa
     3241 ttgattctgg caacaggaga caccttgatt aggacagagg aattagtcaa agatttgccc
     3301 tttgatcagg tctatacaga cttgaaacct gatgggaaat ttgagttagt agagaaactg
     3361 cagaaagcag gtcacactat tttgatggtt ggagatggat tgaatgactc agcggcttta
     3421 actctatcag atatcggtgt ggtgatgaat gagagtgcag atatttctaa gcagatgagt
     3481 gatatcttat tgttagataa tcgtttggat ttcttccaag agttggattc gttatcatca
     3541 tctttgcaaa cactcatcaa gaaaaatatt caagataccg ttgtcgtaaa tagtagtttg
     3601 attggcttcg gcttatttaa ttggctcagt ccttcaaatc tctctatctt acataatcta
     3661 acaaccttgc gcattgtcct gcgtagcctg tctattaaaa atagatagat gagagttatt
     3721 aacagcaaaa aggagttacc tttctcgagg ttaactcctt tgtttataga taaatgttga
     3781 atagtttagt aagtcaatac aaagtatttt ttcttcccac gacggataac agtcagttca
     3841 ttctctaact tatcagcgtc actcaagaca tagtcaaggt cttggatgcg gtcgccgttt
     3901 acgtagatgg ctccgttttg gacgtcttca cgggcttggc gttttgagtt aactatacca
     3961 gatgagacga gcagttccac gatattgttg tgttcgtctg cctgtacttg gtagtttggc
     4021 acaccacgaa gtccttgttt gagttctttg acagaaaggt ttttgatgtt tcctgcaaag
     4081 agttgctcag tgatgttaag tgcttctttg taggcttctt ctccgtgaac aagtgtaaca
     4141 acttcacgag ccaagacttt ttgagccaag cgttcgtgtg gcgctgcttc aaattgttta
     4201 cgaatatctt caatctcatc aagtgacaag aaagtaaaga ttttcaagaa gcgaacagcg
     4261 tcagcgtcca tcacgttcat ccagaattgg tacatttcgt atggagaagt cttttcggga
     4321 ttgagccaga cggcatttcc ttctgattta ccaaatttct taccagttgc atctgtgatt
     4381 agtggaacag tgataacgtg accagtcttg tccgccttac gacgaagcaa ttcggtacca
     4441 gctgtcatat ttccccactg gtcagaacca ccgatttgaa gagtgacatt atggtcttgg
     4501 ttaaggacga agaagtcgta cccttgcatg atttggtaag cgaactcagt gtaagaaatt
     4561 cctgtttcga tccgtttttt aacagattcc ttactcatca tgtagttgac cgtgaagtat
     4621 tttccaatat cacggaggaa gtcaatgaag ctgatgctgc caaaccagtc gtagttgttg
     4681 accatgacag ccttgttttc gccattttca aagtcaagaa aacgagaaag ttgtccttgg
     4741 atagacttga cccagccatc tactgtgtct tttgtttgga gactacgttc agcatctttg
     4801 aaggacggat ctccgatgag acctgtagca ccgccaacga gcgcataagg tttgtgacct
     4861 gctagttgca agcgacgact tgtcaagatt gcgacaaggt ggcctaggtg aaggctgtca
     4921 gcagttggat cgtagccagt ataataagaa acttgacctt cttctagggc tttacgcaaa
     4981 gcttcttcat cagtcgtttg aaatatcaaa ccacgctctt ttagctcatc aaaaatgtgc
     5041 atgtgtcttt tctccttttt aaaattattg tttctaccta ttgtatcaca aacttgggca
     5101 atagcctagc agaataggga agaaagtggc tattttattg aaaatttccc ttttgtggta
     5161 taatagataa agtgaggaaa tccatgcaaa atcaattaaa tgaattaaaa cgaaaaatgc
     5221 tggaattttt ccagcaaaaa caaaaaaata aaaaatcagc tagacctggc aagaaaggtt
     5281 caagtaccaa aaaatctaaa accttagata agtcagccat tttcccagct attttactga
     5341 gtataaaagc cttatttaat ttactctttg tactcggttt tctaggagga atgttgggag
     5401 ttgggattgc tttggggtac ggagtggcct tatttgacaa ggttcgggtg cctcagacag
     5461 aagaattggt gaatcaggtc aaggacatct cttctatttc agagattacc tattcggacg
     5521 ggacggtgat tgcttccata gagagtgatt tgttgcgcac ttctatctca tctgagcaaa
     5581 tttcggaaaa tctgaagaag gctatcattg cgacagaaga tgaacacttt aaagaacata
     5641 agggtgtagt acccaaggcg gtgattcgtg cgaccttggg gaaatttgta ggtttgggtt
     5701 cctctagtgg gggttcaacc ttgacccagc aactaattaa acagcaggtt gttggggatg
     5761 cgccgacctt ggctcgtaag gcggcagaga ttgtggatgc tcttgccttg gaacgcgcca
     5821 tgaataaaga tgagatttta acgacctatc tcaatgtggc tccctttggc cgaaataata
     5881 agggacagaa tattgcaggg gctcggcaag cagctgaggg aattttcggt gtagatgcca
     5941 gtcagttgac tgttcctcaa gcagcatttt tagcaggact tccacagagt cccattactt
     6001 actctcctta tgaaaatact ggggagttga agagtgatga agacctagaa attggcttaa
     6061 gacgggctaa ggcagttctt tacagtatgt atcgtacagg tgcattaagc aaagacgagt
     6121 attctcagta caaggattat gaccttaaac aggacttttt accatcgggc acggttacag
     6181 gaatttcacg agactattta tactttacaa ctttggcaga agctcaagaa cgtatgtatg
     6241 actatctagc tcagagagac aatgtctccg ctaaggagtt gaaaaatgag gtaactcaga
     6301 agttttatcg agatttggca gccaaggaaa ttgaaaatgg tggttataag attactacta
     6361 ccatagatca gaaaattcat tctgccatgc aaagtgcggt tgctgattat ggctatcttt
     6421 tagacgatgg aacaggtcgt gtagaagtag ggaatgtctt gatggacaac caaacaggtg
     6481 ctattctagg ctttgtaggt ggtcgtaatt atcaagaaaa tcaaaataat catgcctttg
     6541 ataccaaacg ttcgccagct tctactacca agcccttgct ggcctacggt attgctattg
     6601 accagggctt gatgggaagt gaaacgattc tatctaacta tccaacaaac tttgctaatg
     6661 gcaatccgat tatgtatgct aatagcaagg gaacaggaat gatgaccttg ggagaagctc
     6721 tgaactattc atggaatatc cctgcttact ggacctatcg tatgctccgt gaaaagggtg
     6781 ttgatgtcaa gggttatatg gaaaagatgg gttacgagat tcctgagtac ggtattgaga
     6841 gcttgccaat gggtggtggt attgaagtca cagttgccca gcataccaat ggctatcaga
     6901 ccttagctaa taatggagtt tatcatcaga agcatgtgat ttcaaagatt gaagcagcag
     6961 atggtagagt ggtgtatgag tatcaggata aaccggttca agtctattca aaagctactg
     7021 cgacgattat gcagggattg ctacgagaag ttctatcctc tcgtgtgaca acaaccttca
     7081 agtctaacct gacttcttta aatcctactc tggctaatgc agattggatt gggaagactg
     7141 gtacaaccaa ccaagacgaa aatatgtggc tcatgctttc gacacctaga ttaaccctag
     7201 gtggctggat tgggcatgat gataatcatt cattgtcacg tagagcaggt tattctaata
     7261 actctaatta catggctcat ctggtaaatg cgattcagca agcttcccca agcatttggg
     7321 ggaacgagcg ctttgcttta gatcctagtg tagtgaaatc ggaagtcttg aaatcaacag
     7381 gtcaaaaacc agggaaggtt tctgttgaag gaaaagaagt agaggtcaca ggttcgactg
     7441 ttaccagcta ttgggctaat aagtcaggag cgccagcgac aagttatcgc tttgctattg
     7501 gcggaagtga tgcggattat cagaatgctt ggtctagtat tgtggggagt ctaccaactc
     7561 catccagctc cagcagttca agtagtagtt ctagcgatag cagtaactca agtactacac
     7621 gaccttcttc ttcaagggcg agacgataat tctctaaatg aagtggccaa tcaatggatt
     7681 gccacttttt tcctttcgtg ttacaataag ggtatgaatc agtatcagaa aaagattgtt
     7741 aacggaaaaa tttattcgct cctatccggc ttaatatggg gaatctgtgg aattttagga
     7801 gagtacttct ttactcatta tcaggtgtct tcgggctgga ttacctctat gcgtttgaca
     7861 ctggcaggga gtcttgtact catttggtct gcaatacaat taaaatcgca agtgctagat
     7921 atttggcgag acaagaaaaa ttacctgccc tttttagcct atgctatttt ggggattttt
     7981 tcagttcagt attttttcta tctctgtgta gaatactcaa atgctacgac agcaactatt
     8041 ttacagttta ttagccctgt ttttatcctc ttttacaatc gcttggttta tcaaaaacga
     8101 gcgtcaaaaa gcgctgtttt ctatgttttg gttgccatgc tgggtgtttg cttgatggcg
     8161 acaaagggag atctctctca gttatccatg acgccgctag ctcttataac aggtttgctg
     8221 agtgccatgg gtgttatgtt taatgttatc ttgccccaac cttttgctaa gcgttatggt
     8281 tttgttccta cggttgggtg ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg
     8341 ccggtttatc agctttcctt tactcttgat atttggagta tcttgatttg cctcattatc
     8401 gctttctttg gaacggcttt tgcttttttc atttccatga aggctgtgtc cttggtttct
     8461 cctttggtgg ttgccgttat cagtgccagt gaacctctct cttctgctct cttgagtgtt
     8521 ttgttcttag gattagtagt ggattggtcc ctccttctag ctatagcctt gattatttta
     8581 cccatgatat ttttgtctat agaagaagcg aaagaaagta gataaaaagt cttttcttaa
     8641 ttctaaaagt gtgctatact agaatagtca ataaaacacg gggagatagt tgtgaagagt
     8701 gtttttaggt tgtatcggta ggggcttgct tttaccgaca gcacgtttta tctcacatcc
     8761 ctaaaaaatc atacctaaaa acagacaaaa aggtttcaaa tttgaggcct tttttggtag
     8821 aataggtatc attataacga accaggaggc acctatgact gctacaaaaa tgaacgctca
     8881 agaaattatc caatttatcg ccaatgctga aaagaaaacc agtgtcaaag taacctttga
     8941 ggggcaactc gcaactgctg tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg
     9001 agactggaag gacgtggctc cgcttcttga aggtttggta gaaaatcaag attatgttgt
     9061 cgagcaagat gctcgtaatt ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg
     9121 tatcgagcca ggtgctatta tccgtgacca ggtgaaaatt ggtgacaatg ctgttatcat
     9181 gatgggagct gttatcaata tcggtgctga aatcggtgct ggaaccatga ttgacatggg
     9241 tgccatcctt ggtggccgtg ctatcgttgg aaaaaatagc cacgttggtg caggtgcagt
     9301 tttggcaggt gtgattgagc cagctagtgc tgaaccagtc cgtgtcggag acaatgttct
     9361 tatcggggct aatgcagtgg ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc
     9421 agcaggagct attgttaccc aagatgtccc agaaaacgtg gtagtagcag gtgttccagc
     9481 tcgtattatc aaagaaattg atgcccaaac tcaacaaaaa acagcgttag aggatgcgct
     9541 tcgtaccttg taattgtaaa agtaaaaaag aggcggaacc ctttttccag cctctttctg
     9601 ctatatcgga ggacagatag atgttagatt tgattcagac tagacgagat ttacaccaga
     9661 ttccagagat tggcttggag gagttcaaga ctcaggctta tttgctggat gtgattgaga
     9721 aattgactac gggcaaggat tttgttcaaa ttcgtacttg gcggacaggg attttggtct
     9781 acctgcaggg aagtcagccg gagcgaacca ttggttggcg gacagatatt gatggcctgc
     9841 ctatcgtcga acaaacaggt ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct
     9901 gtggccatga ttttcatatg accattgcct taggctgtct tgagcgcgcc cttgaggagc
     9961 aacctaaaaa taatctgctc tttctatttc agcctgctga agaaaatgaa gctggtggta
    10021 tgctcatgta tgaggctggt gcttttggag attggttgcc agatcaattt tatggcctcc
    10081 atgtccgtcc agatttgaaa gtcggccaga ttgcgactaa tactcataca ctctttgcag
    10141 gaacttgcga ggtgaagatc cgtttcaaag gtaaaggtgg ccacgcggct tttcctcatg
    10201 aagccaatga cgccttggtg gcggctagtt actttgtgac ccaagtgcag tcagttgtta
    10261 gccgcaatgt aaacccaatc gagggagcag tggtgacttt cggcgttttt caagctggaa
    10321 ccaccaacaa tgtcatcaca gacacagctt ttttacatgg aactattcgt gccttgacac
    10381 aggacatgag tctcttggtg caaaagaggg taaaaacagt tgcagaaggg attgcagctg
    10441 cctttgatat ggaagtcgaa gtggaactca aacaaggtgg atacctacct gttgagaaca
    10501 atccagcctt ggcgcgtgaa ctgatggact tctttgacga aaaagacgga atcgagttga
    10561 ttgatatcga acctgctatg acaggcgagg actttggtta tctcctttca aaggttgatg
    10621 gcgtgatgtt ttggctaggt atcgatagtc cctacgctct tcatcaccct caaatgagtc
    10681 ctaaggaaga agtcttagct attggggtgg ctgcggtgtc tagtttcctg aaaaagaagg
    10741 cagcagaata gagggcttgt ctatgaaatc ggaattacgc aagcaagtct tgcatgaaat
    10801 gaaggcttta tctcaagaac aaaaacaggc tatagaccaa gctttaaccg agcgaatttt
    10861 acaacacccc ttttatcaag aagccaaggt catcgcaacc tacctctctt tttctcatga
    10921 gtttcaaacg cgggaactga ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc
    10981 caaaacttat cccaaggggc gcatggactt tgtagtctat gatccgcagc agttggtaaa
    11041 aactgccttt gccttactgg agccacaggg agatttggaa gtggtggatg tatctcagat
    11101 tgatttgatt catgttcctg gcctggcttt tacgacggat ggatatcgga ttggatatgg
    11161 tggaggttat tatgaccgct atctggaaca tttttctggt catactttga gtacggttca
    11221 tccttgtcaa attcaggact ttatacctga aaaccatgat attcctgttc aggaggtatt
    11281 aattgatgaa ggaaatcttt gatagacgtt accctgtgac gagtttcttc ctcttagtga
    11341 cggccttggt atttttacta atgttggtca ctgcaggcgg aaactttgac agggcagata
    11401 cattatttcg atttggagcc atgtatgggc cagctattcg cctctttccc gagcaggttt
    11461 ggcgtctctt gtctgccatt tttgttcata ttgggtggga acatttcatt gttaatatgc
    11521 tttcacttta ttatcttgga aggcaggtag aggagatttt cggctctaag cagttctttt
    11581 ttctctatct cttatcagga atgatgggca atctctttgt ttttgtattt agtcctaaat
    11641 ccttagcagc aggcgcctct acctctcttt atgggctatt tgccgcgatt attgttcttc
    11701 gctatgcaac tcgcaatcct tatatccaac agctagggca atcctatctg acactttttg
    11761 tggttaacat tattggaagt gttctgattc caggaatcag cctagcaggc catatcggtg
    11821 gtgcagttgg tggcgcattt ctagcagtta tctttccagt tagaggagaa aaacggatgt
    11881 ataataccag ccagagatta ggagcggtag tcttgttcgt aggactcgcc attttgcttt
    11941 tctacaaggg aatgggaatg tgacgaagtt gaaagaaaat ttgtgtaatg aaaaaagata
    12001 aggcattttt tgaaccttat ctttttattt tattttattc cttctcagtc aattcttttc
    12061 caagttggat gaggtaattc ttcaaatcat ctttgacttg tgggtgtttg agggcgtagt
    12121 cgatggatgt tttcatgaag ccaaacttgt ctccgacatc gtaacgagcc ccattgaact
    12181 cacgagcaaa tacacgttgt gttttattga gggtgtcgat tgcatctgtc agctgaattt
    12241 catttcctgc acctggagct tggttttcga ggatttggaa aatttcaggc gtgaggaggt
    12301 agcgtccgat aatagcaagg tcgctaggag cgtcctctgg agctggtttt tcaacaaagg
    12361 tttcaacact gtaaagacca tcttttcctt cgccttgcgg agcaataacc ccgtaagcag
    12421 atacttcgtc atgagggact ggcatgacag cgatagtaga cgcgtgggta cgctcgtagt
    12481 catccatgag ttgtttggta agtggaacag ccttttcgtc tgtgatatcc atcaagtcat
    12541 caccaagcat aacgacaaaa ggttcatttc cgacgaaagc cttggcttgc aaaacagcat
    12601 ctccgagacc gcgtggatga gtttggcgga taaaatgcag acgcatgtca gttgttttat
    12661 caactagctt caaaagatct gttttccctt tttctttgag gttatattcc aattcgaagt
    12721 ttgaatcaaa gtggtcctca atagaacgtt ttgatttacc agtgacaact agaatatctt
    12781 caatacctga tttgagagcc tcttccacga taaactggat agttggtttg tctacgattg
    12841 gcaacatttc tttggcaagg gccttggttg ctggtaaaaa tcgagttcct agtccagcag
    12901 cagggatgac tgcctttcta acttttgatg tcataaaaat cctttctata gagggttaag
    12961 accactcatt ttctgcttta aattcattgt tcatgatgtc ataaatggca tctttgatat
    13021 tggttccgtg ataaataact tggtaaatag cctgtgtaat gggcatatag actccaagtt
    13081 cttgggctag ttcataggct gctcgagtcg ttgaaattcc ttcgattacc atgcccatat
    13141 tagcttctat atcagctagg gattctcctc gtccgagagc atctccagct ctccagttac
    13201 gagagtggat ggaagttccc gttacgatca aatctcccac accagataag ccgctatagg
    13261 tcaatggact ggccccgagt gctaccccta ggcgggtgat ttctgctaaa cctcgagcga
    13321 tgatggctgc cttagcatta tcaccaaatc caagaccatg taaagctcca gcaccgacag
    13381 caataatatt tttaagagca ccagcagttt caaccccgat aacatccgta ttggtataaa
    13441 gtcggaagta gtgattacta aatagcttct gaacgtattg agctgtttgt aaatctttag
    13501 aagcagcagt tattaaagtt aggtcacgca caatggtctc ttctgcatga ctaggccctg
    13561 aaacaacgac gatatcacta cggagatgtt caggaatttc ttcttcaaga atggttgata
    13621 atcgtttatg gctatcaggt tctaatccct ttgatgcgtg catgatgata accttatggt
    13681 ccaaggtttg tgcaacttgc tgggcaacaa gtcgtgtcac ttttgttggg acaacaaaca
    13741 aaatcgcatc cacatctttc aatgtttctg ctaagtcggt gtaggcaatg atattttcgt
    13801 ctagaacgac atctttaaag tagtgcttat tagtatggtg tgtattaatt tcattgattt
    13861 gctcgggaag atttccccaa atacgtacct cgtgtccatt gtcatttaag acttgtgaaa
    13921 gggcggttcc ccaagaacca ggccccaaga cggcgacggt ttgtttttcc atgatttcct
    13981 ccttgataga gttctttctc ttattctatc atattctagg ggattttgca cttgcattac
    14041 tggggtatgg tggaaaaata cacaaaaacc gagatgaagg gataaactct tagtctttag
    14101 ttaaagtgag aagaaatctg atagatagtt tgattcagta tatgctataa atagaggtaa
    14161 acaactcagg aggttcttgt aggttgcgag agtttggcga aaaaattaaa agattacgtt
    14221 tggctaaaaa aatcagtcgt tcagaatttt gtggtgatga gtctgaatta agtatccgtc
    14281 aattaattag aattgaaaat ggagaatcca gaccaacact aacaaagtta aaatatatcg
    14341 ctgaacgttt gggggttgaa gattacaagt tgatgccaag ttatatagag ttggataagg
    14401 aatacctaga attgaagtat ttcttgatga ggactcctac atacgaagat gaaactatcg
    14461 cccaaaagaa agagagtgtt ttgataagat ttttgaagag tattatgata ggctacctga
    14521 ggaagaaaga tttatcatcc caaattattc atatctggca ctaacgaact acacagttca
    14581 aaaattacca gaaaagctag ttgaaatact gtccttctgg tgattttgct taatttctga
    14641 tgttcgagac ataattgagg agatttgtct tgattttttc ctaaaaaagg gcagactcct
    14701 cccttggttt gatgctcgat ttttaaaata aggtgactta ttctgttagg catgttttcc
    14761 cctgccctct agctctatat ggaacaggga gtatcagatt tatctggcag attgtatcaa
    14821 ataaggcttg aaactgtttt gaataggcgt atagacttga gaatttgaga atatgtcgtc
    14881 tagcagtaga gacatatttt ccggcaatat gaaggaagag acgtcgaaaa cgcttgatag
    14941 tcaaggcctt tacttcatca ccagctagct gctttaaaaa gaggtagaga ttataagcca
    15001 gacagcccat catcatacgt acttcattct taatcatggt cgaactatct gtcttatccc
    15061 caaagaatcc tgctttcctt tctttgataa agttttcagc attccctcgt tcacgataga
    15121 attgaaacag ctcctcacta tctacactgc ttaaattggt caaatgaact tttttacaac
    15181 cataaaaaag agcacttaat cgtactcttc tcttttaagg attagttgaa ttaattcaaa
    15241 tccactagtt ctcctgtttc taggtagact aggcgttcac aaatgttagc aatgtaatca
    15301 gcgaagcgtt ccagatgccc tatgatataa aggtattgag ttccattggg aattgaggtt
    15361 tcttggtctt tcataagtcc aatgatttcc tttgataagg cataataata ttggtcaatc
    15421 tgttcatctt tttgagcaat actaatagct tttgaggctt ggtgcaaagg aaaggcaacc
    15481 aataaatcgg ctagcatgct gagggataat ttacccattt ggtgtaactg ttcttcgtca
    15541 ggggctagtt gattttcttt tagttgcaaa acagctttgg caatgcctgc catatggtct
    15601 cccatacgtt caaggtctga acaagaagac atgatgctaa ttacaaatcg aaggtcagac
    15661 acttgtggct gctgcaaggc caacaaacgg gcacaggtca attcgatagc gctttgacct
    15721 tggttgatag catgatcctt attgataatt agctctgcca tctccttgtc tttggaggct
    15781 aaggccagta aggcttttga agctgtttca aggacaagtt gccctagtcc taaaaaggat
    15841 tgttctaatt catgcaattc taagtcaaat tgatttctca taggtttttc cttccttatc
    15901 cgaaccgtcc tgaaatatag tcttctgtgc gctgatcttt tggattggta aacacgtcaa
    15961 cggtatctcc aaattcgcaa atttctcctg ttaagaaaaa agcagtttta tctgaaatac
    16021 gtgaagcttg ttgcatgtta tgggtaacaa tgataatcgt ataatccttt tttagttgct
    16081 gaatgaggtc ttcaattttt aaagtggaga tagggtctaa ggctgaagtc ggctcatcca
    16141 ttaacagaac atcaggttct actgctaaag ctcgcgcaat gcaaaggcgt tgctgctgac
    16201 cgccagataa ggacatggca ctctttttaa gatcatcttt gacttcttcc caaatggctg
    16261 ccccttttaa agatttctcc actaaggcat ctaattgttt tttgtctcga ataccatgtg
    16321 tccttggacc ataagccacg ttatcataga tagacatggc aaagggatta ggctgttgaa
    16381 aaaccatccc tacacgcttg cgtagctgat taaggttgaa tttgctacta taaatatctt
    16441 gctcatctaa gaggacttgg ccttcaatat ggcaagaagg aaccaaatcg ttcatccgat
    16501 taagggtttt tagaaaagtt gatttgccac aaccagatgg gcctatcaag gcagtaatct
    16561 gtctttctgg taattgaatc gaaatatttt ttaaggcttg aaaatccccg taaaataaat
    16621 ctaggtgtct aactgaaaat gttcccatac taggaagctc ctttcacaag tttccgagat
    16681 aataagcttg atagagtatt tatcattaaa acagtaataa tcaaaatcac gccggtagca
    16741 taggcttcat tgacatgtag cccctcactt gacagcatat acatatgtag ggctagagaa
    16801 cggcctgaag acatgagact acttggcgta ttggtagagg tacctaatgt atacatgagg
    16861 gcagctgttt caccaacgat acggccaata gctagtatca ctccagctaa aatacctggc
    16921 atggcaactg gtagaacaat tctaaaaaca gtccgtaact tacctgcccc aagtccataa
    16981 cttgcttgac gcatgctatc actaacagat aaaagggctt cttctgttga gcgaataatg
    17041 actggcaaca ccatgataac tgaggttaag attcctgata acagagagta ttgaaaacct
    17101 aagaagacta caaagaagag catgccaaac agaccaaaaa caatggaagg aatcccagac
    17161 aaggtatctg aggccaatcg catgatttta acacaaaggg aatctttttt tgtatattcc
    17221 acaagataaa aaccagcaaa aatccctatg ggcaaggcta aaagaagagc accaaaaacc
    17281 agaataacgg tggaaataat cgctggcata agggaaatgt tctcagaagt ataagtccaa
    17341 gaaaagaggg atagacttag atgaggtaag cctttgatga ggataaaacc aatgattaaa
    17401 aagagagagc caaaggttaa agctgaaaaa caataaacga gaagttttag caggtattta
    17461 ctcataagat gattttcctt tcaagtaggc aaagtaggca ttaatcaaga gaataaggaa
    17521 aaagagaact gctgaggttg caataagggc ttccctatgc tgacctgatg cgtaagccat
    17581 ttccagaaca atattggttg ttaaggttct ggttcctgaa aagagtccac ttggaataat
    17641 cggctggttg cctgccacca aaataactgc catggtttca cctactgcgc gaccgattcc
    17701 taaaataact gctgataaaa taccagatct cgcagctggc aagatgacac taaaaatact
    17761 ccgttcatga ctagctccta gagccaagct accagaataa tacgttttgg gaactgttcg
    17821 gatagcagat tctgacaaac tgataatggt tggcaaaatc attattccta atagtaacga
    17881 agcggttagg acactcatgc catttcctaa aaaggttcta atccaaggca ccaataattg
    17941 taggccgaaa aaaccataaa caatagatgg aatggccgcc atcaagttga tagctgattt
    18001 taagaagcca tagacgggct ttggacaata ataaaccata aacaccgatg tcaagatgcc
    18061 tgttggcacc ccaatcacaa tcgctcctaa ggtaattaat aaggaaccaa cgatcattgg
    18121 taaaatacca tagcttgccg gaatgttcgt tggcgaccaa tcactgccta ataaaaaacg
    18181 ggcaaagccg tagttagcca tgaaaggtaa gccattacta aaaataaaga aacagattag
    18241 caaaatagct acaacagcta ctgttgcact catgaaaaaa attgccctaa aaactgcttc
    18301 tttgaaggct tgttttatca catcttgtcc tttctagtga agaaagtaag ggagatacga
    18361 cacctcccta cttgccttct ttatcttatt gtacgatgaa acgtctgcat ctctttagag
    18421 atttatggag caaacatttt atttaatctt gtcccaggtg gttaatttgc cactaaaaac
    18481 gtctgcaagt tcagccatac tgacttggct tgccttattg tcattattga ccacaacagc
    18541 aataccgtct aaagcaatag catcatgggt gagactctta ccttcttcag gagttaattc
    18601 cctagaaacc ataccaatat cagcggtttt ctccttaaca gcggtaatac ctgctgaaga
    18661 cccattagag gtaatatcaa tcgtaacttc tggattttct tttttataag cttctgctaa
    18721 tttttccatt aaagaagata ctgaagtgga acctacaaca gacaacttgc ctgataagtg
    18781 ttggcttgta tattctgtgg tttcggtttt agcttcaata aatttattat ctgtgaccac
    18841 ttgttgacct tgtttggagt ggataaagct gataaaatct tgacctagct tggaaagatt
    18901 agaagaccaa acaatgttga agggacgttg aagagggtat tcaccatcta aaactgtgtc
    18961 tcgactagcc ttgacaccat caatctctaa agccttgaca gattttgtta aagatcccaa
    19021 ggagatgtag ccgatagcat tagcattccc ttgaactgct gagagaacac cttctgtact
    19081 attttgaatc acagctgttt tggcagtgtt gtcaattttt ttatcaccgt cttttttgag
    19141 aatccctgtg atttctgtga aggcaccccg tgttccagag ccattttctc gtgaaatcac
    19201 ctcaatcgtt cctgaagctg actgtttgga agcagctgac tgattgccac aggcaacaag
    19261 cccaaatcct gataagccaa tggctgcaag agtaagcatt tttttgaatt tcataataat
    19321 cacctttatc tctatgtatt tttcttgtgt aggcttacta catttatagt ctaacaagtc
    19381 tttgtaaagg tttatccctg attcatgtaa agattgtgta aagaatcaaa aaaagccact
    19441 tttgaaaaat ggctgctcct aaaaatagct ttaaaaatta ttagtcctgt gcgaaagatt
    19501 ggttaggaag aaaaatcgtg aagcaactgc ctctgccaag ctgactcgtc accgtgactt
    19561 ggccacctaa taattgactg agttctttga caatggcaag gccaagacca gtgccaccag
    19621 tttgtctgct tcgaccttta ttaactcggt aaaaacgttc aaaaatacga tcctgctcta
    19681 attgactaat accaatccct gtatctgata cagaaatctt aatgccttcg ttcacctttt
    19741 gggtcttgac ctcaattttt cccccttgtt cagtgtaacg gatggcattg gataaaagat
    19801 tgagtaagat ttgggaaagt aattgactat ctgatacgag ggtgacatca tctggcacct
    19861 gcacctttag ctgtaaatcc ttcttcttga gctgaggttg caagctttga gtcaaatcct
    19921 gtacaaattc tgccaaagaa agggtcgtcc attgtatagg catttgttga gccttagata
    19981 aggtaagaag atgctcaaca atatgctcaa gacgcaaact ttctttgtaa ataatgtcta
    20041 gaaagtcatc cttgagcgct tcttcttcag ctgacatccc cttaatggtt tcagcaaagc
    20101 ccttaatcga agtaactggt gtcctcaatt catgggaggc atttgagaca aaggctaaat
    20161 ttaacttttc ataagttcta atcgttgtta aatcatatag caagacgagc acagcttcca
    20221 cagattgggt ggggctaaaa acgggaactg ctgtcacttc taaaatcaag tcaccctcat
    20281 gaaacccact tacttcttgt tttaaccttg ttttttgatc aaaggcttgg tgaactaaat
    20341 tccgaatatc catccgtttg aggtcatcaa gtgaacttat gtcgccgtcc acatcgggaa
    20401 aataatgagg cagagagcga ctggataata acatctgacc ttgagcggaa actaaaaacg
    20461 tccccatggt taggtgcgac agaagaacct ccattgtttc ggctagatcc ttgtattgct
    20521 gatcctgttg ggagactttg gtttttaggc cagacacata ctgagccaaa gactttaagt
    20581 cttcttgccc tttttctaaa aagtattcac tactggtcaa gagaggttgg tgcaaggtct
    20641 caaaagcaac ttcccatttc caaaggcaaa agagccagta gccacctagt cccaaagaaa
    20701 gggctagaag aaagagaccg atgcctttac tgatccaagt taatgccatc cctgcaatca
    20761 gaatgaggct aacacttaga ttgactagcc aaaattgaag gtagcgtttc atctataact
    20821 ccttgaactt ataaccataa ccccgaatgg ttcgaataaa ttgaggggct ttaggattgt
    20881 cttcaatttt ttccctcaac ttaccaatat gaacgtccac caaacgtgtt tcctgcccaa
    20941 agtcataccc ccagatacgt tccaaaagac gctctctagt cagtgtcatg ttgggatgtt
    21001 tcataagata gagcaagagt tcaaattctt ttggggtcaa actcagtaac ttattcgcct
    21061 tgtagacttc atgacgctca gggtatactt tcaaggtccc aaatagccaa gaatcgtcag
    21121 cgatattatc tgaatcatct ccttcttgtt ctcctttagt tcgcctgagg acagccttga
    21181 cacgcgccag caattctcta gggctaaaag gcttggtcag gtagtcatca gcccctaatt
    21241 ccaaggccaa aaccttatca aattcatcac ttttcgcaga aaccatcata attggagttt
    21301 tgacgccttt ggctctcagc cgcttacaaa cttccatgcc atctaattgt ggtaacatga
    21361 tatcaagcaa gataaaatca aagggttctg tttctgccaa agctaaggcc ttccgtccat
    21421 ttgtcaccaa ttgagtagaa aagccttcct tacttaaatg gtagtcaagc aatttcagaa
    21481 tgtgttcttc atcatccact aataagactt gttttgtcat ctattatctc ctattggtaa
    21541 cattataaca caattatcag aaatcctaac attgctaaat cagattaaat ttgcctatca
    21601 agactagtat ctggtcaaac gctcaatcat ctccttgtgc tctggatagg tcgccagtag
    21661 atctaccctt tcaaataatt caaaatcctc aaattcaaaa ccaggagcaa caagacaaga
    21721 aaccagagca tcatccttat caactgttga tccccaaata gtgcccttag gaacacagta
    21781 gtgaagttgt tgccctttgg atatgtccag gcctaaagtg actgcttcgt agtgaccatc
    21841 tgctgtaatc atgtgaacag taagtgggga tcctgcatga aaataccaga tttcatctgc
    21901 tgtcaatcgg tgaaaatgtg aaggattcgt ttcttctaat aagaaataaa tactggtata
    21961 aagcgccctt cccttaccag caaggtttat agtgtctgaa gctttttttg tttgtctaaa
    22021 atagccacct tcaatatggg gagctaactc tagagttctt atcaagtctt ctttatccgt
    22081 cggagccaat gggttgaagt aactcttgtt caaagtggtt ttacgatttc aagaactcct
    22141 ctcagttctg aggacacggt aatgattgat gcgacggaag tacaaatcaa tcgccctaaa
    22201 aaaagaatta gcgaattatt ctggtaaaaa aaatgccacg ctatgaaggc tcaagcgatt
    22261 gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactattg tcatgatatg
    22321 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt
    22381 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
    22441 cttaagccac taacagttga agataaagcc tataaccatg cgctatccaa ggagagaagc
    22501 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgatttcaac aacctatcga
    22561 aatcatcgta aacacttcgg attacgaatg aatttgattg ctggcattat caatcatgaa
    22621 ctaggattct agttttgcag gaagtctatt atttggttag gtgaattagt gaagcgttta
    22681 ggcaagtgtc tctggttacg acgtcatgga ctctaaatcg attatattta ggggtcatga
    22741 ctagtgaagc agttagctag ttcgcatata agcggctagc gtctaacaat taggaacttt
    22801 agttccaata actttaagat tacgacgttt taggacataa atcgatcata tttatgtcct
    22861 aaaactagtg aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg
    22921 ctttgcaatc aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag
    22981 cgaagggctt ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt
    23041 tctgggcttt catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg
    23101 ctaattgcaa atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata
    23161 cgtgggaagt cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag
    23221 ttgccagctg tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag
    23281 gcatattgaa cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag
    23341 tcgtatccat ttgtacggtc ggttttgagg tcatagaatt taatggctcc aatcccaaca
    23401 gcatgtgcta cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg
    23461 gcacggctaa cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta
    23521 gagagtttct tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc
    23581 cagtcgtagc ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct
    23641 tgaccaacga catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca
    23701 gccaagtcac gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt
    23761 tcaattccat atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct
    23821 ttttcagaaa gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta
    23881 tagctgtcaa attcaacctt caattcattg taaaggcggt taaattccac taaactttca
    23941 tcgcggaacc attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac
    24001 cattcgcgcg cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca
    24061 tagagtttaa ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt
    24121 ttgtaggcaa caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg
    24181 accgtttgat aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa
    24241 cgcaggtggc caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca
    24301 ttttcttgtt taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc
    24361 aatacttgag cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg
    24421 acaacttttt caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt
    24481 ggtgctttac gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct
    24541 gagtttttag gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg
    24601 ctagccaatt cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt
    24661 tctactattt tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt
    24721 ttggtataat atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa
    24781 aaagagatcg tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa
    24841 aagaaattca agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc
    24901 gtgatttgcg cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac
    24961 tagtaaatga gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg
    25021 ttgcaagagc agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg
    25081 caaatattgt agatgtaaac aaggataaat ggattttagg aacagttgct ggtgccaata
    25141 ccttattggt tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag
    25201 atttgatgaa agataagtaa ggtcttggga gttactctca agacttattt ttgaaaagga
    25261 gagacagaaa atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa
    25321 aaagcaatat ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta
    25381 tgaggatgcg gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa
    25441 tgccgacaat ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga
    25501 tgtcttgatt gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca
    25561 agcagttggg gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga
    25621 tagcagtaag ccggacagtc agaataattt tttggtttcc atagaccgcg aaggcaatca
    25681 atttggccta gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga
    25741 tttcacgctg gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta
    25801 tgacttgtct gaggaagaag aacaaatcct cagccgccag atgaatctgg tactctctta
    25861 tgaaaaagaa agctttgaag accttcattt attggatttg cgattggcaa cggtggagca
    25921 aacggcatct agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca
    25981 cctcaaacct gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa
    26041 ggctagtctg gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg
    26101 gcttttggat gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg
    26161 ccccttgatt gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga
    26221 ccatttcttt gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg
    26281 cttggctagt cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac
    26341 taccttgtct agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctactct
    26401 agcctatctc atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc
    26461 gattgctcct gaagctcctc atgtgattac agatggggga attatccgga ctggatttga
    26521 tgagacttta gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat
    26581 tgaggctaag gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa
    26641 ggatggctac tattttcatg tgaccaattc gcaactagga aatgtgccag cccacttttt
    26701 ccgcaaggcg acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga
    26761 gggagatatg cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg
    26821 cattcgtgaa gaggtcggca agtacatcca gcgtttacaa gctctagccc aaggaattgc
    26881 gacggttgat gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc
    26941 tgagtttggt gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa
    27001 ggttatgggg gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat
    27061 tcaactgatt acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat
    27121 gacggcggtt atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat
    27181 ttttgatgcg atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcgac
    27241 ctttatggtg gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct
    27301 cattctcttt gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca
    27361 gtccatcatc gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta
    27421 ccatgagttg actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac
    27481 tctggagcag gatgggcagg ttaccttcct tcacaagatt gaaccgggac cagctgataa
    27541 atcctacggt atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc
    27601 ggataagatt ttgactcagc tagagaatca aggaacagag agtcctcctc ccatgagaca
    27661 aactagtgct gtcactgaac atatttcact ctttgatagg gcagaagagc atcctatcct
    27721 agcagaatta gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt
    27781 agtagagtta aaacagaaac tataaaacca agactcacta gttaatctag ctgtatcaag
    27841 gagacttctt tgacaattct ccactttttt gctagaataa catcacacaa acagaatgaa
    27901 aaggagctga cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt
    27961 cagaaaataa aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg
    28021 gttgctagtt cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact
    28081 gggaaatatg aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt
    28141 ctagttgctg gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc
    28201 gaccttcggg aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa
    28261 tttaatgcgg gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt
    28321 gtcatgatga ccttccaaat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc
    28381 ctagcggttc aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt
    28441 tttggtttga ctgctgtcat gatgggaata atggggcctc gttttgccaa gtttcaaacc
    28501 cttcttgagc gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag
    28561 tcctttgtcc aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt
    28621 ggtcaaaacc tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt
    28681 ggttacgggg cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg
    28741 tctgttgttg gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt
    28801 gttatggttg gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt
    28861 cgagaaattc ttgacgcaga gccagctatg accttcaagg atatcccaga tgaagagttg
    28921 gttggaagtc ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg
    28981 ctgaaagttg tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact
    29041 ggtgcaggaa agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggacggg
    29101 gccattaaaa tcggtggcaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca
    29161 gtttccatcg ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga
    29221 caggggaagg ggaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct
    29281 agtgaattca ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc
    29341 aatttctctg gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca
    29401 cgtattctga tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg
    29461 caagaagctt tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt
    29521 agctcggttg tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa
    29581 ggtacgcatg cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag
    29641 aaatgaaaga caaactataa gaaaagtcaa tagttttatc taaactattt cttatttcaa
    29701 tttgatgatt tggcgatgat tttagagcac ggcaaaaggc ccttgaaaaa gtccattttt
    29761 tcaaaggtaa tcctgtgtta atttcagaaa ttacatcact ttttgttcgt caaatggcag
    29821 ctcttttttt aggatataaa acagggttcg gataagtttt tttgcaaggt ggatgatggc
    29881 tacattgtaa tgttttcctt gttctaattt agtcttaaga taggccttaa aagcaggcga
    29941 aaagcgaggg catgctttgg cagcttgtat gagtacctac cgcagatgag gggaactccg
    30001 tttgaccatt cttcctgcta aatcaatctg atctgactga taaatagaag aatccagtcc
    30061 agcgaaagct tgtaattgag caggattatc aaaggcatga atatttcgaa tctcagctaa
    30121 aatgaccgcc cctaaacgat ccccaatccc agtaaccgtc gtgatgaccg agttgaactc
    30181 agccatcaag tcattgacac atgtttccgc cttgtcaatg agcctcttgt aatgtttgat
    30241 gttttcatta cacgagataa aacgtctatg cgttatcaaa ctcattacca attaaaacaa
    30301 aaagctgtgg ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg aactaatcca
    30361 cagcggctta ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata
    30421 atataaggag gagtaaaatg aagacagttc aatttttttg gcattatttt aaggtctaca
    30481 agttctcatt tgtagttgtc atcctgatga ttgttctggc gacttttgcc caagccctct
    30541 ttccagtctt ttctggacaa gcggtgacgc agctagccaa tttagttcaa gcttatcaaa
    30601 atggcaatcc agaacttgta tggcaaagcc tatcaggaat catggtcaat cttggcctgc
    30661 tggttttggt tctatttatc tctagtgtaa tatacatgtg tctcatgacg cgcgtgattg
    30721 cagaatcgac caacgagatg cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt
    30781 ctttctttga ccgtcgacaa gatggcgata tcctgtctca ttttaccagt gatttggata
    30841 atatcctcca agcctttaac gaaagcttga ttcaggtcat gagcaatatt gttttataca
    30901 ttggtctgat tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc accattgcca
    30961 gcaccccatt ggctttcctt atgctgattt tcatcgtgaa aatggcacgc aaatacacca
    31021 acctccagca gaaagaggta gggaagctca acgcctatat ggatgagagc atctcaggcc
    31081 aaaaagccgt gattgtgcaa ggaattcaag aggatatgat ggcaggattt cttgaacaaa
    31141 atgagcgcgt gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt cttttccctg
    31201 tcatgaatgg gatgagcctg attaatacag ccatcgtcat ctttgctggt tcggctgtac
    31261 ttttgaatga taagtctatt gaaacaagta cagccctagg tttgattgtt atgtttgcac
    31321 aattttcaca gcagtactac cagcctatta tccaagttgc agcgagttgg ggaagccttc
    31381 agttggcctt tactggagct gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc
    31441 gacctgaaaa ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg
    31501 atttttcata cttgcctgat aaacctattt tgaaagatat cagcatttct gcccctaaag
    31561 gccagatgac agcagttgtt gggccgacag gttcaggaaa aacgactatt atgaacctca
    31621 tcaatcgctt ttatgatgtt gatgctggtg gtatttattt tgatggtaaa gacattcgtg
    31681 gctatgattt agatagtctt agaagcaagg tgggaattgt attgcaagat tcggtcttgt
    31741 ttagcggaac gattcgagac aatatccgat ttggtgtgcc agatgctagt caggaaatgg
    31801 ttgaggtagc agcaaaagca acccacattc acgactatat cgaaagtttg cctgataagt
    31861 acgatactct tattgatgat gaccagagca tcttttcaac agggcagaag caattgattt
    31921 caatcgctcg aaccctgatg acagatccag aagttctcat tctcgatgaa gcaacttcaa
    31981 acgtagatac ggtgacagaa agcaagattc agcatgccat ggaggtggtt gtagcaggta
    32041 gaactagttt cgtcattgcc caccgcttga aaaccattct caatgcagat cagattattg
    32101 tccttaaaga tggagaagtc attgaacgtg gtaaccacca tgaacttttg aagctaggtg
    32161 gcttttattc agaactctat cacaatcaat ttgttttcga ataagaaaga agttgtccta
    32221 tgtgggcagc tttttcttgt ccataaaaaa tgtttatcac agccttaaaa aaaacatatt
    32281 agacgaaagt cattttgagt gatatgatag gactatcgtt agcattcgaa aggagaggca
    32341 tcatggctag aacggttgta ggagttactg caaatctatg tcccgtagac gcagaaggca
    32401 aaatcattca ttcatctgta tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc
    32461 tccctttagt cattcctgtt ggtgatgagt cagttgtacg tgattatgtg gaaatgattg
    32521 acaaactcat tttgacagga ggccaaaatg ttcatcctca gttttatgga gagaaaaaga
    32581 ccgtcgagag cgatgattac aatctggtcc gtgacgaatt tgaattggca ctcttgaagg
    32641 aagcgcttcg tcagaataaa ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg
    32701 ttgcctttgg tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg
    32761 ggacatctca ctctattgag acagtagaag gaagcgtggt ggctaagcta tttgggaaag
    32821 aaagtcaggt caattcagtc catcgtcaaa gcattaaaga tttggcaccc aatttccgtg
    32881 taactgctat tgattcaaga gaccaaacta ttgaagcgat tgagtctatc gacgagcacc
    32941 gcattatcgg tttacagtgg catccagagt ttctggttaa tgaagaagat ggcaatttag
    33001 aattatttga gtatttattg aatgaactgt aacgattgga atatctggtc gttctttctt
    33061 ttattttttc aaaattttta gaatgtggta ttttacgcaa acgtttgaat tctgataaaa
    33121 attggagaaa ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat gatagaaaag
    33181 aagaaatatt ggaggaagaa catgtcacat attaaatttg attattcaaa agttttagac
    33241 aaatttgttg caccacatga agtggaatac atgcaatcac aagtaacagc agcagatgaa
    33301 ttgatccgta aaggaactgg tgctggtagc gacttcttgg gatggttgga ccttcctgaa
    33361 aaatacgacc gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac
    33421 agcgatgttt tggttgtgat cggtattggt ggatcttacc ttggtgcaaa agcagcaatc
    33481 gacttcttga atcaccattt tgctaacttg caaacaaaag aagaacgcaa agctccacaa
    33541 atcctttacg caggaaactc aatctcatct acttaccttg ctgacttggt agagtatgtt
    33601 gcagacaaag acttctcagt aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg
    33661 attgctttcc gtgtctttaa agaactcttg gttaagaaat acggtcaaga agaagctaac
    33721 aaacgtatct atgcaacaac tgaccgccaa aagggtgctg ttaaggttga agcagacgct
    33781 aacggttggg aaacatttgt tgttccagat gatatcggtg gacgcttctc agtattgaca
    33841 gccgttggtt tgcttccaat cgcagcatca ggagctgaca tcaaagctct tatggaaggt
    33901 gcgaatgcag ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga agcttaccaa
    33961 tacgcagctg ttcgtaacat cctttatcgt aaaggctatg caactgagat cttggtaaac
    34021 tatgagccat cacttcaata cttctcagaa tggtggaaac aattggctgg tgaatcagaa
    34081 ggaaaagacc aaaaaggtat ctacccaact tcagccaact tctcaactga cttgcactca
    34141 cttggtcaat ttatccaaga aggaactcgt atcatgtttg aaacagttgt ccgtgttgac
    34201 aaacctcgta aaaacgtgct tattcctact ttggaagaag accttgacgg acttggttac
    34261 cttcaaggaa aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc
    34321 cacacagatg gtgatgtacc aaacatgtat gtgactcttc cagagcaaga cgctttcact
    34381 cttggttaca ctatctactt cttcgaattg gcaattgccc tttcaggtta cttgaatgct
    34441 atcaacccat ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt tgcccttctt
    34501 ggaaaaccag gatttgaaga attgagcaaa gaacttaacg cacgtctata atagaagaaa
    34561 agagtggttt acccactctt tttactctct ttatccatag aaattggact cagtcaagac
    34621 ttgtgatata atatagaaag caaaaaggca gatgcctaga taataggaga aactatgtca
    34681 aaagatatcc gcgtacgtta cgcaccaagt ccaacaggac tactacacat cggaaatgct
    34741 cgtacagcat tgtttaatta cttgtatgcg cgccatcatg gtggaacatt tctcatccgt
    34801 atcgaagata ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca acttgaaaac
    34861 cttcgctggt taggcatgga ttgggatgaa agtccagaat cacatgagaa ttatcgccag
    34921 tctgagcgtt tagacttgta tcaaaaatac attgaccaac tattagctga aggaaaagcc
    34981 tataaatctt acgttacaga agaagagttg gcagctgaac gcgaacgcca agaagtagct
    35041 ggcgaaacac cacgctacat caatgaatac cttggtatga gtgaagaaga aaaagcagct
    35101 tacatcgcag aacgtgaagc agcagggatc atcccaactg ttcgtttggc tgtcaatgag
    35161 tcaggtatct acaagtggca tgatatggtc aaaggcgata tcgaatttga aggtggcaat
    35221 atcggtggtg actgggttat ccaaaagaaa gacggttacc caacttacaa ctttgccgtt
    35281 gttatcgatg accacgatat gcaaatctct catgttatcc gtggagatga ccatattgct
    35341 aatacaccaa aacagcttat ggtctatgaa gctcttggtt gggaagctcc agagttcggt
    35401 cacatgacct tgattatcaa ctctgagact ggtaagaagt tatctaaacg tgatactaat
    35461 acacttcaat ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc agtctttaac
    35521 tttattgctc ttcttggttg gaacccaggt ggtgaagatg agattttctc tcgtgaagaa
    35581 ctcattaaac tttttgatga aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa
    35641 aaactagact ggatgagcaa tgattatatc aagaatgcag accttgaaac catctttgaa
    35701 atggcaaaac cattcttaga ggaagcaggc cgtttgactg acaaggctga aaaattagtt
    35761 gagctctata aaccacaaat gaaatcagta gatgagatta tcccattgac agatcttttc
    35821 ttctcagatt tcccagaatt gacagaagca gagcgcgaag tcatgacggg tgaaacagtt
    35881 ccaacagttc ttgaagcatt caaagcaaaa cttgaagcga tgacagatga taaatttgtg
    35941 acagaaaata tcttcccaca aattaaagca gttcaaaaag aaacaggtat taaagggaaa
    36001 aatcttttca tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca
    36061 gatacaattt tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa
    36121 gaaatctcta aataaagagg atacaataat ggacatctgg gaaaagatgt acgaagaagc
    36181 acagaaacta tacaatccac atgaagtatc agactttgtt tatgccaatc atgttgtagc
    36241 tgcagtagaa gcagaagatg gacaaatatt tacaggattc tgtatggagg gaacctgtgg
    36301 tgttttccat ctctgcgcag aacgggcggc cctcttcaat atgtaccaat tttcaggaca
    36361 aactaaggtt aagaaagtat tagcctttcg agacaaacca ccttatggtg gaagttcagc
    36421 catgccttgc ggtgcttgta gagaattcct tttagaactt aatgctgaaa ataaagatgc
    36481 agaattcatg atggactata atataagaaa aacagttaaa gtcgcagaac taatccctta
    36541 ttggtgggga gaagaacgtg cttctaagtt taatgagcga tagaagagtc agtataattt
    36601 tggctctttt tactataagt tgaaaaaat
//
LOCUS       DAVHIU010000006        36616 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVHIU010000006 DAVHIU010000000
VERSION     DAVHIU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.006"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            19..1473
                     /locus_tag="VVN06_000242"
     CDS             19..1473
                     /locus_tag="VVN06_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU5561856.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFIEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            1454..2419
                     /locus_tag="VVN06_000243"
     CDS             1454..2419
                     /locus_tag="VVN06_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5561857.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILIIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2454..3728)
                     /locus_tag="VVN06_000244"
     CDS             complement(2454..3728)
                     /locus_tag="VVN06_000244"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU5561858.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3728..4900)
                     /locus_tag="VVN06_000245"
     CDS             complement(3728..4900)
                     /locus_tag="VVN06_000245"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU5561859.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(5031..5234)
                     /locus_tag="VVN06_000246"
     CDS             complement(5031..5234)
                     /locus_tag="VVN06_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561860.1"
                     /translation="MNLIAEKVSKTLDKTFDKDVREIPTSQFYQKFVDEMGRTYSGNL
                     ILQELITVNGAYKATYIGELSSN"
     gene            complement(5220..5783)
                     /locus_tag="VVN06_000247"
                     /pseudo
     CDS             complement(5220..5783)
                     /locus_tag="VVN06_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5845..6294)
                     /locus_tag="VVN06_000248"
     CDS             complement(5845..6294)
                     /locus_tag="VVN06_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5561861.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(6296..7054)
                     /locus_tag="VVN06_000249"
     CDS             complement(6296..7054)
                     /locus_tag="VVN06_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5561862.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7169..9148)
                     /gene="pknB"
                     /locus_tag="VVN06_000250"
     CDS             complement(7169..9148)
                     /gene="pknB"
                     /locus_tag="VVN06_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU5561863.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(9145..9885)
                     /locus_tag="VVN06_000251"
     CDS             complement(9145..9885)
                     /locus_tag="VVN06_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5561864.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(9900..11213)
                     /gene="rsmB"
                     /locus_tag="VVN06_000252"
     CDS             complement(9900..11213)
                     /gene="rsmB"
                     /locus_tag="VVN06_000252"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU5561865.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDISSIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11206..12141)
                     /locus_tag="VVN06_000253"
     CDS             complement(11206..12141)
                     /locus_tag="VVN06_000253"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5561866.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12154..14550)
                     /locus_tag="VVN06_000254"
     CDS             complement(12154..14550)
                     /locus_tag="VVN06_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5561867.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14616..14930)
                     /locus_tag="VVN06_000255"
     CDS             complement(14616..14930)
                     /locus_tag="VVN06_000255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5561868.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(14955..15581)
                     /gene="gmk"
                     /locus_tag="VVN06_000256"
     CDS             complement(14955..15581)
                     /gene="gmk"
                     /locus_tag="VVN06_000256"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5561869.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(15711..17324)
                     /locus_tag="VVN06_000257"
     CDS             complement(15711..17324)
                     /locus_tag="VVN06_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5561870.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLINEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(17593..17823)
                     /locus_tag="VVN06_000258"
     CDS             complement(17593..17823)
                     /locus_tag="VVN06_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU5561871.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
     gene            complement(18061..19158)
                     /locus_tag="VVN06_000259"
     CDS             complement(18061..19158)
                     /locus_tag="VVN06_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU5561872.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(19168..19914)
                     /locus_tag="VVN06_000260"
     CDS             complement(19168..19914)
                     /locus_tag="VVN06_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5561873.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKITFIEGNMLNLSNAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(20017..20370)
                     /gene="rsfS"
                     /locus_tag="VVN06_000261"
     CDS             complement(20017..20370)
                     /gene="rsfS"
                     /locus_tag="VVN06_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5561874.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(20383..20883)
                     /locus_tag="VVN06_000262"
     CDS             complement(20383..20883)
                     /locus_tag="VVN06_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU5561875.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(20884..21477)
                     /gene="yqeK"
                     /locus_tag="VVN06_000263"
     CDS             complement(20884..21477)
                     /gene="yqeK"
                     /locus_tag="VVN06_000263"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU5561876.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(21477..22106)
                     /locus_tag="VVN06_000264"
     CDS             complement(21477..22106)
                     /locus_tag="VVN06_000264"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5561877.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(22158..22469)
                     /gene="yhbY"
                     /locus_tag="VVN06_000265"
     CDS             complement(22158..22469)
                     /gene="yhbY"
                     /locus_tag="VVN06_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU5561878.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(22683..23789)
                     /gene="yqeH"
                     /locus_tag="VVN06_000266"
     CDS             complement(22683..23789)
                     /gene="yqeH"
                     /locus_tag="VVN06_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU5561879.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVIRKAII"
     gene            complement(23792..24382)
                     /locus_tag="VVN06_000267"
     CDS             complement(23792..24382)
                     /locus_tag="VVN06_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU5561880.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(24334..25242)
                     /locus_tag="VVN06_000268"
     CDS             complement(24334..25242)
                     /locus_tag="VVN06_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5561881.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIG
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(25299..25859)
                     /locus_tag="VVN06_000269"
     CDS             complement(25299..25859)
                     /locus_tag="VVN06_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU5561882.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            25998..27206
                     /gene="sstT"
                     /locus_tag="VVN06_000270"
                     /pseudo
     CDS             25998..27206
                     /gene="sstT"
                     /locus_tag="VVN06_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            27274..28227
                     /locus_tag="VVN06_000271"
     CDS             27274..28227
                     /locus_tag="VVN06_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU5561883.1"
                     /translation="MSISQRTTKLILATCLAYLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            28370..28750
                     /locus_tag="VVN06_000272"
     CDS             28370..28750
                     /locus_tag="VVN06_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5561884.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            28849..29070
                     /locus_tag="VVN06_000273"
     CDS             28849..29070
                     /locus_tag="VVN06_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU5561885.1"
                     /translation="MIEITYLDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            29087..29401
                     /gene="trxA"
                     /locus_tag="VVN06_000274"
     CDS             29087..29401
                     /gene="trxA"
                     /locus_tag="VVN06_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5561886.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            29706..30239
                     /gene="queF"
                     /locus_tag="VVN06_000275"
     CDS             29706..30239
                     /gene="queF"
                     /locus_tag="VVN06_000275"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU5561887.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQHHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            30289..30717
                     /locus_tag="VVN06_000276"
     CDS             30289..30717
                     /locus_tag="VVN06_000276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561888.1"
                     /translation="MLKAVFEVIDKTDEVSLKHCFEVADRTDEVSLKHCFEVVDETDE
                     VSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVVDET
                     DEVSLKHCFEVVDETDEVSLKHCFEVADRTDEVSNIYTTR"
     gene            complement(30807..31475)
                     /locus_tag="VVN06_000277"
     CDS             complement(30807..31475)
                     /locus_tag="VVN06_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU5561889.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGYLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(31586..32140)
                     /locus_tag="VVN06_000278"
                     /pseudo
     CDS             complement(31586..32140)
                     /locus_tag="VVN06_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            complement(32386..34182)
                     /gene="pepF"
                     /locus_tag="VVN06_000279"
     CDS             complement(32386..34182)
                     /gene="pepF"
                     /locus_tag="VVN06_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU5561890.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(34193..34936)
                     /locus_tag="VVN06_000280"
     CDS             complement(34193..34936)
                     /locus_tag="VVN06_000280"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU5561891.1"
                     /translation="MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(34938..35888)
                     /locus_tag="VVN06_000281"
     CDS             complement(34938..35888)
                     /locus_tag="VVN06_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5561892.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(35903..>36616)
                     /locus_tag="VVN06_000282"
     CDS             complement(35903..>36616)
                     /locus_tag="VVN06_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5561893.1"
                     /translation="YLRIQQEKNLEKGNAIDFISTLDDMRTSLPVINQPAIDKKYNEN
                     PHQDDIVNIVTLNYTHVIDKLYNGSAKSFRNQLRANLYNFYIEPPIHAHGTVDVCTVL
                     GVSDEIQISNSLEEQKESLIKSLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDR
                     YIWSQIAERSISGSVPVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKK
                     LRDNLIVVIGKTIFDLIER"
BASE COUNT        10147 a         8506 c         7211 g        10752 t
ORIGIN      
        1 agatttttgg aggaatgaat ggaatggaca actgagcgtc gttacagact ttatcaagat
       61 tggacgcaag aagaaattca acatataaag gaaaatatgg cacaatctcc atggcatact
      121 cattaccatg ttgagccaaa aacaggactt ctcaacgacc caaatggctt ttcttacttt
      181 gatggcaagt ggatcctctt ttaccagaat tttccttttg gtgcagccca cggtttaaaa
      241 tcttgggcac agctagaaag tgatgatttg attcacttta aagaaactgg aatcaaagtt
      301 ttaccagata ctccattaga tagccacggt gcctactctg gttctgccat gcaatttggc
      361 gataacttat tcctatttta tacaggaaat gttcgcgatg aaaactggat ccgtcaccca
      421 taccaaatcg gtgctttgat ggacaaggag ggtaagatta caaagattga caagatcttg
      481 attgaccagc cagcagactc tactgaccac ttccgcgatc cacaaatttt taactttaaa
      541 ggtcaatact atgctgtcgt cggtggacag gacttggaga aaaaaggctt cgtccgtctc
      601 tacaaggctg tcaataacga ctacacaaac tggcaagcag ttggcgacct tgactttgct
      661 aacgaccgta ctgcctacat gatggaatgt cctaatctgg tctttataga ggaacaacct
      721 gtccttctct actgtccaca aggattggat aagaaagttc tagactacga taatatcttt
      781 ccaaatatgt ataagatcgg ggcttccttt gaccctaaaa atgccaaaat ggtagatgtg
      841 tctcaacttc aaaacatgga ttacggtttc gaagcctatg caactcaagc cttcaacgct
      901 cctgatgggc gtgctctagc agttagctgg cttggtttgc cagatgtttc ttacccatct
      961 gaccgttttg accaccaagg aaccttctct ttggtcaagg aactcactat caaagacgac
     1021 aagctctacc agtatccagt cgctgctatt aaggaccttc gtgcttctga agaagccttc
     1081 tcaaaccgtt cccaaaccaa gaacacttac gaacttgaac tcaacttgga agctaatagc
     1141 cagagcgaga ttgtcttact tgctgataaa gaaggtaagg gactttcaat caactttgac
     1201 cttgtaaacg gtcaagtgac agtggatcgt agccaggctg gagaacaata tgcccaagaa
     1261 tttgggacaa ctcgttcttg ccctatcgag aatcaggcta ctactgctac aatcttcatc
     1321 gataactctg tctttgaaat tttcatcaat aaaggagaaa aagtattttc tggtcgtgtc
     1381 ttcccacatg cggaccaaaa tggtatcctg atcaaatctg gaaacccaac tggaacttac
     1441 tatgaattag attatggtcg caaaactaac tgatgtcgcc aaacttgcag gcgtcagtcc
     1501 tactaccgtt tctcgggtta tcaataaaaa aggatatcta tctgagaaaa ccatccaaaa
     1561 agtcaatgaa gccatgagag aattaggcta taaacccaac aacctggctc gtagtctgca
     1621 aggaaaatca gctaagttaa tcggcttgat tttccccaat atttccaatg ttttctatgc
     1681 agaattgatt gataaattgg aacaccaact cttcaaaaat ggttacaaga ccatcatctg
     1741 caacagtgaa catgattctg agaaggaacg cgaatacatc gaaatgttgg aagccaatca
     1801 ggtagacggc atcatttctg gtagtcacaa cctaggaatc gaagactaca atcgtgtgac
     1861 agcgccgatt atttcctttg accgaaacct atcgccagac atccctgttg tctcctctga
     1921 caactatgct ggtggggttc ttgctgccca aaccttggtc aagacaggtg cccagtctat
     1981 catcatgatt acagggaatg acaattccaa ttcgccaacc ggactgcgcc acgctggttt
     2041 tgcatccgta ctcccaaaag ctcctattat caatgtttcc agtgactttt ctcccgtcag
     2101 aaaagaaatg gaaatcaaga atatcttgac ccgggaaaaa ccagatgcca tttttgcttc
     2161 ggatgatttg acagctattc tgatcattaa aatcgctcaa gaattgggca tttctgtccc
     2221 aaaagagctc aaggttatcg gctatgatgg gacctacttt atcgaaaatt actaccctca
     2281 attggctact atcaagcaac ctttggaaga gattgcttgt ctcactattg atcttctctt
     2341 gcaaaagatt gaaggcaagg aagtcgccac aactggttac ttcttaccag ttacactatt
     2401 accaggaaaa agtatttaaa cacaagaaaa ctcagaccga ttcgtctgag tttttatgat
     2461 cttaaatttt cgagatagcg ctgggctgtc tctaggttaa aggttttatc tgagatgagg
     2521 cgctctacta ggggagcaac ttcagattca ctagccccag ctaggagagc tagggatttg
     2581 gcctgtagtt tcatgtggcc ttgctggatg cccgtactta ccaaggcttt gagggctgca
     2641 aaattttggg caagaccgat ggacacgata atctgggcta attctctggc agaaggattt
     2701 cctagtagat catgactgag agctacacgt gggttgaggc cgatagagcc acccttagtc
     2761 gctacaggca tgggcagggt catctcaccg accaattctt ctctttcaag gtccagcgtc
     2821 cagcaactaa gaccttgata gcgtccatct cgactggcaa aggcatgggc cccagcttcg
     2881 atggcacgcc agtcattacc agtggcaatc aaaatcgcat caataccatt aaaaattcct
     2941 ttattatgag tagcagctcg gtaaggatca gcctgcgcaa actgactagc caacgcaatt
     3001 ttctccgcaa tctctcgtcc ttgatccttt tggcggctca agtagcgaaa ggcgatgcga
     3061 cagcttgcag tcaccagaga atcggtcgcg tagttggaca ggattcccat gagactctgt
     3121 ccctgactga gttcttctaa gactggtttc aaggcttcca gcatggtgtt gagcatattg
     3181 gcacccatgg cttcctgggt atcgacatga atataaacaa cgagaaagtc tggttcgcct
     3241 tttatctgct cgacatgcag atcacgcgcc ccacctccac gtttaacgat agaaggatag
     3301 gcttgattgg caagctccaa gagctccgct ttcttgctgg caatcttctc ttgcgctagt
     3361 ttaggattag caacttgata aagggctacc tgcccaatca tctgtcgctg atggacttgt
     3421 gcagtaaaac cacctgcacg cttgatgatt ttgctggcat agctggccgc cgcaaccaca
     3481 gagggttctt ctgtcacata gggaacggtg tattcctgac cgttgacaag tacctccgga
     3541 accagtgaat aaggcagaga aaaagttccc actacattct cactcagctg gtctgccaca
     3601 gtcacgctca tctgttcatc cttctccaga ctagcttgtc tctcaggact aaggagcgcc
     3661 tgagctttta acagctcgag gcgctcttgg tatgattttt tagaaaatcc attccaactt
     3721 atcttcatta tttttcaacc ttgctataac ggcgttggtg gtcgagaatt tcaaccaagg
     3781 caaaatcttg attttcatag ccagcaaact gggcagagtt tgtttcatcc aagtttactt
     3841 cctcaaaaaa gaccttttca tagtctgcaa cggataagac agttcgttgg ttgagcttgt
     3901 tcaagcggtc tttatccaaa taagcttcat atccttcaac caattcacca ctgaagaact
     3961 cagccacagc tccacttccg taactataaa gggcgatttt atccccagct ttcaagctat
     4021 ctgtattttc caagagagac aaaagtccaa ggaaaagtga acctgtgtag atattcccca
     4081 ccttttgact gtagagaata gactggtcaa aatgcttttg taagaggtct tttttctctt
     4141 gaggcaggct cttatccatg atttttttca agccttttag cgctaattta ggataaggca
     4201 agtggaaaca aacagccgca aaatcatcca aagtaagctg gtagcgtttt tgatattcaa
     4261 gccaagtcgt tttcaaacta tccaagtatt gttgggtaga atagacacca tttacataag
     4321 gagttgtcga gtaatttggt cgccagaaat ccatgatgtc acgggtctga gctacattgt
     4381 cattattaaa ggccatcatg cgtggatttt gtgtaatcaa catagccaca cttccggcac
     4441 cctgagtggg ttctcccgga gtttcaatac cgtatttggc aatatcactt gcaatgacca
     4501 agaccttgga ctccggagaa ttttccacat gcaatttggc ataatggagg gcagcagtcg
     4561 ccccatagca ggcttcttta atctcgaaac tacgagcaaa gggctggatg cccagcaagc
     4621 catgcacaaa gacggccgca gccttactct ggtcaattcc tgactcggtc gccacaatga
     4681 ccatgtcaac ttcttgtctt tcttgctcag ttaaaataga gtcactagca ctggccgcca
     4741 aggtcacgat gtcctcagtt aggggcgcaa tactcaattc cttgagtaag agtcctttac
     4801 ttaatttttc agggtcaatt cccctcgctt ctgctaagtc ttgtaatttc aagacatatt
     4861 gactggtcgc aaaaccaatc ttatcaatac cgattgtcat atttacctct gttttatcat
     4921 tcatgtaaaa aatcgttcta tactatttta tcacaaatgg cagtaaaaga gagaaaaaag
     4981 acttgattca ccaaatcaag cctcttattg gtcatcattt taaagaatga ttagttgcta
     5041 gagagttcac cgatataagt agctttataa gctccattca cagttatcag ctcctggagg
     5101 atcaaatttc ctgagtaagt ccttcccatc tcatctacaa atttttgata aaactgactg
     5161 gtcggaattt ctctgacatc cttatcaaat gtcttgtcaa gtgttttact aaccttctca
     5221 gcaatcaaat tcattcgtaa tccgaagcgt ttacgatgat ttcgataggt tgttgaaatc
     5281 attttaaacg tttttacttt ggcaaagatg ttctcaacct tgcttctctc cttggatagc
     5341 gcatggttat aggttttatc ttcagctgtt agtggcttga ggatatatct tcatgagccc
     5401 ttgataacca ctgtcagcca agattttacc agcttgtccg atatttctgc aactcatttt
     5461 gaacaacttc atatcatgac aatagttcac agtgatatcc aaagaaacaa ttctcccttg
     5521 acttgtgaca atcgcttgag ccttcatagc gtggcatttc tttttaccag aatcattcgc
     5581 taattctttt tttagggcga ttgatttgta cttccgtcgc atcaatcatt accgtgtcct
     5641 cagaactgag aggagttctt gaaatcgtaa caccaccttt gaacaagagt tacttcaacc
     5701 cattggctcc gacggagtaa gttgctttcg tggataccaa aatcagccgc aatttcttca
     5761 taagtgcggt attctcgcac atatagaaag cgttgttaat ttatttatct catttttcag
     5821 aaaattcttt tatttctgta aagtctacga tactcgatgt gtttttatat aatgatacag
     5881 tctgagaatc actgttccgc tagccattcc aatagagatt accaaagcca acatgacaac
     5941 caaggtcgca cttgccagtg ctttattata gtcccctgtc acaaaaaagg cagttgttcg
     6001 gtaggagaga taacctggaa ccagcggtgc caaaatggcc aagataaaga ccacagcagg
     6061 tgtcttataa agaatactta aaatctggct gacacaagaa ccaataatgg ctgcaatgaa
     6121 ggtagctaca atgacattgg tcggttcctt gagcaagaga tagattagcc agacagtcat
     6181 gcccaaaatc cctccaggta agagcataga ccgttgcaca ttgagtacaa ttaaaaaagt
     6241 gataatggca agaaaacttg ctactgcttg taataaaaag gttgttagtg tcatattagt
     6301 tcatcaatac caaggcgaca gaagttcctg cccctaaagc gagggtaatg agcagggatt
     6361 caaacatctt actcatacca gagtttatgt ggttggtcat aatatcacgg accgcattgg
     6421 tcaaggcaat acctggtaca aacggcatga ccgcaccagc tataatcaaa tctgccgttg
     6481 aaggaaaacc tgtgtagcga gcccaaaact gggcaattat cccaaagaca aaggctccag
     6541 caaaggctgt cacaaaggga attcggataa atttttccac atagagggaa aaggcaaaac
     6601 caaataaggt cgccactcct gccccaagtg cgtcgtagat atttccgcta aacataactg
     6661 aaaagaaagg agcactaaag gtcgcagcca gagttacctg caacttagta tagggaaggg
     6721 gttgagcttg caaggccgtc aattgcttaa aggctgtttc taagtcaatc tgccccccaa
     6781 ctagctgacg agaaatctgg ttcacatcgc agactttttc aatgttataa gaagaggagg
     6841 tcacgcgctt catgcgcgaa atattggtat tttcaataga gaaaaagata gcggcaggca
     6901 tggcaaggac attgcaatcc acaatcccct gcgaatgcgc gattcgaatc atggtatctt
     6961 ctacacgatg gatttctgag ccacttttaa ggagaatagt ccccgctagc ataatcacat
     7021 caatgacggc atttaattct tttgattctt ccatgctttc ctccttttat caactccctc
     7081 tattctatca caaatccgga ctcaaaaaaa atctttgcca tgaaatcatg acaaagattg
     7141 attactcatt ttgattatcc atctgctttt aaggagtagc tgaagttgtt ttaggtttgt
     7201 agattgaaat cttgactcta gtcttattga ggtctacctt ttcacctgct ctaggacttt
     7261 gttcaacaac catgccttct gcactacctg caggcgctgt cgtcacttct acaacttcta
     7321 tattagcttc cttaatccca acaatttgaa tcaaattgtt cttagtaaac tccaagctgg
     7381 aaccaatgta actcggcatg gcaacacttg taactttttt agctactgtc aagacaattt
     7441 gagtaggttt actcacatca taagtcgttc cggcacctgg actttgtttc ataatcgttc
     7501 ctggttcgct ttcgctggac tcttcttcct ctatcttaat caaattctca ggaaccttct
     7561 tctgcttaag ttctgagatt acttctgtag agttccgtcc aatatagttc cctaattgaa
     7621 tcgtcgtagc ttttttagct actgtcaaaa caatttgagt tgccttgctc aagtcatagg
     7681 tcgtaccttc tggtagactt tgcttcaaga ccgttccagc ctcactctca ttcgactctt
     7741 cttcctcaat tttaatcaaa ttatctggaa cttttttctc ttttaattcc gcgataacat
     7801 cagaagattt ccggccgaca taattactaa tttggaagga ttgtttgcct gatgagacaa
     7861 ccaaattgat tttcgttcct tcttttcgac cagttccagc gccaggatct gtacggataa
     7921 tccgcccttc ttccaccttt tcactagcct ctgtcttctc ctcaccaatc tcaaaattgg
     7981 cttttttgag cgttgccttg gcctctgcaa ctgtctgacc tgccacatct ggaatggcaa
     8041 tggttgcagg agttctggat agtatccaaa taagagaagc tgccaccaat acaaggctgg
     8101 ccaacaaaat caggtaacgc atcttaaatc tatgtttttt cggtgcttgt ggttggtaag
     8161 tttcctctgt cacagcctga cttgggtttt tgattgattt gtgttctgtc tgcgcttgaa
     8221 ccttaggaat agatgtcaag gtactctgag aaaccttcgg caaggtcttg gtatctgcct
     8281 tgctcgtttc atcaaagatt aacttacttt catttctacg attgtaggac aagctactag
     8341 acaagtccac atacatctct gaaaccgagc ggtagcgatt ggtcaacttt ttagcagttg
     8401 ccttgataat aacattttct aaagcctgag gtacagatgg attttctgca ataacggacg
     8461 gcaggggttt ctggaaatgc tggagggcaa tggtcaccgc gctatccccg tcataaggga
     8521 tatggcctgt caacatctca tagaaaataa tccccatggc atagatatca ctctgcacag
     8581 tcgccttcga accacgcgcc tgctctggtg acaagtaatg aactgagccc aacatcgagt
     8641 tagtctgggt cagacttgtc tctgcaaagg ctacagcaat cccaaagtct gtgaccttgg
     8701 cagtcccatc tggtgtcaaa aggatatttt gaggtttcaa gtccctgtga acaattcctc
     8761 gagtatgggc caagcgcata gccaagagaa tttgtcccat gatacggact gcttcttcat
     8821 tagaaagagg ataatgttcc ttgatatagc gtttgaggtc cagtccagcc acatactcca
     8881 tagctaggta ctgttgaccg tcttcctcgc caatatctgt tatccgaacg atatgaggat
     8941 ggtctagatc tgccatagct ctcgcttcac gctgaaaacg agctacagct atcgggtccg
     9001 tctggtagtt ggtcctcaga accttcactg ccacttcttc cccatctaag attaagtctt
     9061 tggctaggta gacatccgcc atgcctcctc gaccaatctg tttgacaatc cgatagcgtc
     9121 cggcaaaaat cttgccgatt tggatcattc tgcatcctcc tcgttcatag aaacaagggc
     9181 aaccgtaatg ttgtctaaac ctcctgcatt gttagcaaaa cgaacaagtg tctccgtttt
     9241 atctgctaaa ggaatatcac tggttacaat atcacgaatc tcactgcctg aaatcatgtt
     9301 ggtcaagccg tcactattga gcaagagata gtcacctgac tcaaggataa ctgtcccaaa
     9361 atcaggctga atttcatctt tttgcccaat agactgggtg ataatatttt tttgcggatg
     9421 agcttctgcc tcttctggtg tcaattgacc agccttgagc aattcattaa ccaaggaatg
     9481 atcgctcgtc aactgatggt attcttctcc acgaatcaag ccgatacgcg aatcaccaat
     9541 atgagcatag atagcctgat tatcaataat agcaaggact tccaaagtag ttcccatgcc
     9601 tctgtaagct tcatcctgac caagctggtg aatcttttga ttttcaattt ctaggtaatg
     9661 ggcgaaccat tcacgcactt cattgactgt atcgatctgg gtatcaaccc aagctacacc
     9721 caggtctgtg accgccattt cactagcgat attccctgca cgatgacctc ccatcccatc
     9781 agctaaaata atcatggtac gtccagctct attgacatag tggttgacat agtcttggtt
     9841 atttgttcgt ttctgaccaa catctgttaa taatgaaatt tccatgtgtc agttccttcc
     9901 taatccgata tcttgcgaaa ttgactgatg aagaatccat cacttccata caattcaggt
     9961 gtaatgagga tacagccgtc tttcatgata tccttacatt catgttctag ttttacctgc
    10021 tcgaactcgg gatgactctc taaaaaggcc ttaacgactt gaaaattctc ctctgagacg
    10081 atagtgcagg tgctataagt tattatacca cctttgccta gtatttgaca aacactacct
    10141 aatatttcta actgaatttc ctgcaaggac gcgaaatctg ccgtttcttt attgtatttg
    10201 atatctggtt ttcggcgcaa aagaccgatt cctgaacaag gagcatccac caaaatctta
    10261 tcaaaggaat cctggtcaaa aaactcatgc acctttctgg catccaattt ttgagtttga
    10321 acccgatctt caactcccag acgttgggca ttttcttgaa ttaaatccaa cttgtggtcg
    10381 tacaagtcca gagcagtaac ctgacctgtc gtaagataag aggctatatg ggctgttttc
    10441 ccacctggag ccgcacaggc atcaagcact cgctcatcac cttgtaaatc aagcgtcgga
    10501 gcaaccagct gactggactc gtcttggatg gtaatggctc catccgcaaa caagttatgc
    10561 cctgcaaaat gcccttgctc cttaaccaga ccagtggttg ctaaaaggga attattcgcc
    10621 tccaacaagg cttggatttc ctcttttcga cttaggtctg ttacacgaat actggctttg
    10681 tttcgcacta acaagctttc aaagatggct tttgctctct cctctccgta ttcttccttg
    10741 agtttggcaa ctagccaaac tgggagagaa taggcaatgg agtcacgctt gttttttcgc
    10801 ttgatgctag aaatatctgg ccagccttca cgcaagatac ggcgaaggag ggcattgacc
    10861 aatttttcac tgcctttttt acgaagtttg gccaattcca ctgcttcatt gaccacagca
    10921 tgatctggaa tcttgtccaa atagcggagt tggtaggcac tcatgagaag aaggacatag
    10981 agccagctgt ctaactggtc tctgtcttcg ataaagtggg ataggtacca ttccagagtc
    11041 agtttacggg ctaccgttcc atagaccagc tcggtcacta agcccttgtc tgctgccaaa
    11101 agttgactcc cctttagatg cttatttaag gcgatatttg agtatgcttg gttcacaaaa
    11161 acatcctcta gcactgctag agctaaactt ctagccgttt ctactttagt caccaaatcg
    11221 ttctcctaca gtcaatgtac gtccaactcc gttgaggaag gaagcaatgt ccatcttagg
    11281 cttaccagct ggctgcactt gtttgaggga tagagcccct tcagccgttg cgacaatcaa
    11341 ttctttcttg ccgatagaga gaatctcacc tggatttccc tgaccttcta ctggtagggc
    11401 ttcataaatc ttaaagcggt cgcccttaag gaaagtatgg gcaacaggcc aggggttcat
    11461 tccacgaatt tggttaaaga gttgacgatt ggttttgttc cagtccagtt tttcttcctc
    11521 tggctttata tttggagaga aggtaacctg actcgtatcc tgcggttcag gtttgatatc
    11581 accagcaata taggcaggca gagtgtccaa aagcaaatca cgaccaacta gcgccaattt
    11641 ttcaaacaag gtgccaacat tgtcctcatc tgtgatcgga atgctgcgac gagaaatcat
    11701 atctcctgca tccatttcct taaccatttc catgatggtc acaccagctt cctcatcccc
    11761 ttgaatcaag gcataatgga taggcgcacc accacggtgt ctaggaagga gggaggcatg
    11821 aacgttgaca gcaaagtcca tgctatcaag gagtttgctt gggagaaact gcccaaaagc
    11881 agcagtcaca attccatctg ctcctagctt cataagatct tccatctctg gacttccaga
    11941 taatttttca ggttggtaga tagatagtcc tgcttccttg gcagcctgct tgactggggt
    12001 ttcttggata acttttttac gaccaacagc acggtctggc tgggtcacaa cggctagaat
    12061 ttcgtaacgg tcatctgtca aaagtccttt taagactgtt gctgaaaagt cgggggtccc
    12121 cataaagatt agttttgtca tatcttctcc ttcttataaa aattgctgcg gctcatggtc
    12181 aatgctgaga cggagctcac tattttcccg ttcttgagtc aaggccaaga cctggttgag
    12241 ggtcggcccc agctcatctt ctaaacggta tttaattaaa atctggtaat gatagaggtt
    12301 gtgggtacgg gcaatcggtt ttggcgttgg ccccagaatg ggactggtct ctgacaagcc
    12361 tgaccgcaaa atgttcatga cttcataggc acgtttgaaa acctcttctt ctttcttgtg
    12421 agaaagggta ataccaatcg tgaaatagta aggtggatag ccgagttgtc gtctgattcc
    12481 catttcatag gcataaaagc cttcgtaatc ttgatccttg gcaaatcgaa tagcatagtg
    12541 ctgcgaattg taggactgta tcaagacttg acctgctttt tcagcccgac ctgctcgacc
    12601 tgccacctga gtcaagagct ggaaggttct ctcagaagaa cggaaatcag gcagattcaa
    12661 ggccgtatcc gcatttagaa ctccgactag ggtaacattg ggaaaatcca aaccctttgc
    12721 aatcatctga gtaccaagta aaatatccgc ttcccctcgc ccaaactggt caagcaaggc
    12781 ttggtgactg cctttctttc gagtcgtatc cacatccatc ctcaaaatgc gagcttgggg
    12841 aaagagttct gctagctcat cataagcctt ctgagttccc gtcccatagt aacgaatact
    12901 gcggctctta cagttaggac agacctgagg aatatccttc gagaaaccac aataatggca
    12961 gttcatagtc ttggtatcca tatgcaaggt cagagaaata tcgcagttgg gacaagtatc
    13021 caccgtccca cactcccgac acatgacaaa gctagaataa ccacggcgat tgagcatgag
    13081 aaccacctgc tcttttttaa ccagacggtc ttggatagcc tctagcaaag gaggcgtaaa
    13141 gtttgacgtc tcattttgtc cgatatagtc tcgaaagtca atcacttgaa cctcagggat
    13201 tgtagccaaa ggattggcac gttgggttag acgtaagtgt tgatagacgc ctttgccagc
    13261 acgtgcccgg ctctctaagc tcggcgttgc agatccaagt accagagttg cttgattata
    13321 ctgagcccgt aaaatagcta cctctctggc atggtaacgg ggattgctgt cctgcttata
    13381 agccgcttca tgctcttcat caataatcat gacacccaga tttttcagag gagcaaagat
    13441 agcagatctg gcaccaacaa caacttgggc atcgccacgc tccaccttgc gccattcatc
    13501 atacttttca ccattggata atcctgagtg aagaatggct accttgtccc caaaacgtgc
    13561 tataaaacgc tcggtcatct gaggagtcaa ggaaatctca ggtaccagca aaatagctgt
    13621 cttgccctta tccagggcac cttggataat ctgcaagtaa acctcggtct tcccacttcc
    13681 tgtaatccct tgaagtagaa agggaggttg agaactgcca atagaactca caaccgcatc
    13741 acgcgcctgt ctttgttctg gatttaactc caaaggtcta cttgcttcaa ttccttcaaa
    13801 ataagcagcc gagcgttgaa cttccttttg gactatggta acagcacctt gatccacaaa
    13861 gaagttgact tgctctcgcg agtaggactc taacaagcta gccaaggaag cgctctctgg
    13921 atgagacagc agataatctc tcagttccaa ctttttcttg gcacgtgtag aaatctcaac
    13981 accttctaat tgagcatggt caacctcata ccaagactgg gtcttgacct tcttttgatc
    14041 gactgcctga tattccagaa caagcaggcc ttttctagtc aaacgcatca tttcagcttg
    14101 cttggcaagg tctagtgaag aaaaggctag cgaatcttct gaaccaaaca ggcgcactcg
    14161 ttcttcctga ctcaagcctt ccagaggata gagaatcttg tcatagctag aattcagaaa
    14221 tcctggaagc atggccttga ggatagaaat tttgtaggag aagacagatt tgcgtaactc
    14281 ctcagccagc cagagttgtt ctggcgtgag aacaggagaa aaatccagca cctctgcaat
    14341 atcttttaaa tcttgctcca tctcttctcc atctgattgg gactccaaac caagaacaat
    14401 cccttgaatc aggcgattac ccttaccaaa aggcacatga acccgcatcc caacttccag
    14461 cattccctca aattcctccg gaatcctgta actatagggc tggtccgtct gcatcaaggg
    14521 cacatctacg ataatcttag ctagggccat cttctcacct cctccttgtc agtacattct
    14581 tgcaatagaa aaaataagat tgagtccccc caaccttaaa ttttttcacc atcttctttt
    14641 tctttagcaa tttgctcttt gattttcttt tcttcttctt ctttgcggcg tttttcttct
    14701 tcgatacggc gacgcactgc ttcacgtttt ccttctggat ctgggtgaat tgtaacgttt
    14761 cctgattcga tttcttctaa agcgcgaaga gttgattttt cagacttgaa accttgagtt
    14821 gctggggcac ctgcttccaa ttcgtgggca cgttttgctt ccaagattac gagtgaatat
    14881 tttgaaggaa ccttgtcgag caaggtatca atagagggtt ttaacatcat ttgcttgtac
    14941 ctattttcta aattttatcg ggtagttgga gattttggta acatctcctg atagtgacca
    15001 atgacacgat ccacacagaa gtgttctgct tcaatcacac atttgacacg ttcagcagct
    15061 agggacacct gatcgttgac aatggcataa tcatactcac gcatgagggc aatttcttcc
    15121 ttggcctttt cgattcgttg ggcaatcact tctgcactat ctgttccacg acctaccaag
    15181 cgatcttgca attcatccaa atctggtggt gtcaggaaga taaagacagc atctggaacc
    15241 tttttcttga cctgaagagc accctgaact tcaatttcaa ggaaaacatc gattcccttg
    15301 tccaaggttt cattgacata ggtcagagga gttccatagt agttaccgac atattctgcg
    15361 tattccaaca tctgtccttg acgaatcagc tcttcaaatt cttcacgagt acggaagaaa
    15421 tagtcaacac cgtccacttc tccaggacgt tgtgcgcgtg tcgtcatcga tacagagtat
    15481 tgaaattggt tttcagaact ctcaaaaatc tctcttctaa ccgttccttt tccaacccct
    15541 gaaggaccag aaaaaacgat tagtaagcct cggtctgcca ttgtgtctcc ttttagtcaa
    15601 tctgtgaaat aacatttctc tagaataatg gcaaaaagcc agattatcct ttacagtctt
    15661 tctatctagt gtaacaaaaa agcagtaatt tttcaactgc tctttcttat ttatttagca
    15721 taatctactg cacgaagctc gcgaatcacg gttaccttga tatttcctgg ataatcgaga
    15781 ttgttttcaa ttttcttacg aactttgtga gccaagattg tgactttgtc gtccttgatt
    15841 tttcctggat tgaccatgat acgaatttca cgtcctgctt gaagggcaaa gctagtttgc
    15901 actccttcaa agccgttagc aatttcttcc aaatcatgga gacgcttgat gtagctttca
    15961 agagactcac tacgagcacc tggacgggct gcgctcaagg catctgctgc agcgacgata
    16021 actgctatca cgctctcagc ttcaacatct ccgtggtgac tagcaatcgt attcaccaca
    16081 actgggggtt ccttgtactt acgggccaat tccataccga tttcaacgtg gctaccttca
    16141 acctcatggt caatggcttt cccgatatcg tgaaggaatc cagcacgacg ggcaagagcc
    16201 gcattttcac caagttcgct cgccatgata ccagccaact tagcaacctc aatcgaatgg
    16261 cgcaaaacat tttgtccata tgaagtacgg aactgcaaac gtcccataat cttcatcaag
    16321 tctggatgaa ggtttggcgc accaatttca taggcagcag cctcaccgta ttcacgaatc
    16381 ttattgtcaa tctcttgacg gtttttctca accaactctt cgatacgagc tggatgtata
    16441 cgaccatctt tgagcaacat ttccatagtc atacgggcaa tctcacgacg aatcggatca
    16501 aatcctgaca aggtcaccac ttctggtgta tcgtcgataa tcacatcgac ccctgtcaaa
    16561 ctttcaaagg tacgaatgtt acgaccttca cgaccaataa tgcgtccctt catagtatcg
    16621 tctggcagat gaactgttga gtttgttgac tccgctacat attcaccagc gatacgttgc
    16681 atagcttgaa ccaagatgtc cttggccatt ttgtcagaac gttccttgac ctcttgctca
    16741 gcttcgcgaa tgcgactggc aatctccctg gtcaagtttt cctctgtctg agccaagata
    16801 atatctcgtg cttctgcctg agacagagca ccaatacgct ctagttctgc ttctttttgt
    16861 ctttcgactt cctctaattg ctcttcacgc gcatcaaggt ttttcgctct atcagaaata
    16921 ctttgttctt tttgttcaag tgtttgttct ttactcgtca aattgtcgtc cttacggtca
    16981 aggctagtag ctctctctgt caaacgactt tcgatttgtt tgagttcttg acgttctgat
    17041 ttgaattcag cgtccacttc ttcacggtat tttctggctt cttctttggc ctccaatagt
    17101 gcttcttttt taagagactt gctttcacgt ttggcttcat taataagtaa atccgcttca
    17161 cgctcagctt gtccacgtaa attagttgct tcttgttcag catttaaaag catcaactct
    17221 gcagcttcct gagatgattt catcttagct gagatgctga catatccaat gactaaacca
    17281 atgatgacgg caaaaacagc aatcgcaagc gacatgatct ccatgttttt acctcatttt
    17341 attgttattc cgaatgacat acattctttt acattctacc ataaaaaagt gattttcaca
    17401 aacctaaaat agaatatgtt ttggggaatt tggaacacat ttaccaaaat aaacttgttt
    17461 tttagaaata gtagtttagt agagacttga gaaaaagcct actttgcagt agacttccta
    17521 atgattatga aatacaagaa gtttgtttct aacaacagca agatttcact caggtattct
    17581 ctgtaaatta gactagtgtc ctgcgcgaat tttatccatt cctcgtaaaa ccttgtctga
    17641 taactcctta ttttgggcga ttctcagggt ttcctgaata ctgtcccatt cgctctttga
    17701 aagaactaca atgtcctcat ctggattttt attgaccacc gtcaaaggct caaattcatc
    17761 atttaccttc ttcatgtagt cctttaaatg atttcggaat gttgagtaaa ggactgcttc
    17821 cataaccata cctcgtttta gctcttttcc actattatac acgaaaagaa agaaattgtc
    17881 aggaacttgt acaagatttt cttttctatc tatttatact caatgaaaat caaagagcaa
    17941 actaggaaac tagccgcagg ctgtacttga gtacggcaag gcgacgttga cgcgatttga
    18001 atttgatttt cgaagagtat tattcgtaaa aaatctcaaa aagtccacct ttcggtagac
    18061 ttagtttgtt tctattctaa tcggcactct tccaaaattt tgctctgcta tacttggctt
    18121 tcctagttgg taaatctggt ctgctttttg ggtcatagca tcccaaggtt ctttgccaat
    18181 tcggctgact agactgacct gacctttcaa agacttgaga tgttgcctac ctttttcagt
    18241 aaatccaagg acatgaatgg cttctggcaa gacattttct ctagcctgca tcaaaatata
    18301 ggtcaatagg cgtctgacac gcgccttggt gtaacgtttg gtagtcacta actcgaccaa
    18361 ttcttccaca gactgggctg ttttaatagc ttctttaatg cgcacagcca tttcttgatt
    18421 gacctgatag atggtggtta agtcgggatt tgacaagatt tgatagcgga gcaagggaaa
    18481 atagtcttcc cagatcacct tactggcctg ctcaaatagg gcaacagaag gcataaaacg
    18541 ttctaagaaa tcttggtcct tctgatgttg acggagggct gtcgccgagg caaagtccac
    18601 atctttattc acagaatggt aacctgcccc ctgacgctga atcggatgca gcttgatatt
    18661 ccgtcctgca accgccttgg cataggccag agcaagaaca tgattggggg tattacctga
    18721 aaaatcaaga ccagcaaatt ccttccacat agcttgggtt ttctggggat aggaaaggga
    18781 atcaggcaga ttttccacaa atttctccat ctcagtacct ttctctgtgt ataagtcagc
    18841 gattttctga taatctcgaa cttcctctgt cccaaaaact agactatcaa tgcccaagcg
    18901 atccaagata tccacagctc cttgaccaaa gaaatcagct gcctgaacac tgactaaaaa
    18961 gggcaattcc actaccaagt ctgcgccatt ttccagcgcc atctgggccc ttgtccactt
    19021 atccacaata gcaggctctc cacgctgcat gaaattccct gacatggcaa caattttcag
    19081 tccctctgcc tgatccagca ggtatttatg gccattatga aaaggattga actctgcgat
    19141 aatacctgtg atggtcatga tgttctccta cttctgcgcc acaaaaaacc aacgggtgct
    19201 agtttctgtt ggctccttat cctcaaagtc tgcatagagt ttgaaagact taaatccagc
    19261 ctgttccagc aagatatcat aggtcaagat ctcataggtc ctctcctcat gtacttcatc
    19321 atgacgacta aaggaaccgt cagcctcctt gataaagaaa gtcagctcat gcacgatgga
    19381 gtgaggagct tcgtcctcat atgtatccca aagcatggca aaatcttccg cattttcatg
    19441 gtaggaatag ccagggaata cttcatctgt ctggtaggtc gagtgcacgt caaagatgaa
    19501 aactccctct tcattcagcg cattgtacac gtccttaaag acgtccccta cttccacctc
    19561 atcctgcata tagcagatag agtccgaata acacgtgaca aaatcgtatt tccctgcatt
    19621 ggacaaattc agcatattac cttctataaa agtaatcttt tgcttggctg aagttgctct
    19681 cttctccgca atcttcaaca tatccgcact caagtcaagt ccagtcacat caaaaccagc
    19741 ctgagagaag cgcactgatt gaattcctgt cccacaagcc aattccaaga gtttctttct
    19801 ctccttggtc ttaggcaaat gacgcagaga aaaattcgtc catttgtcgt ataaactatc
    19861 gtccatgacc gcatcataga ccgccgcaaa ggtttcataa gtcgccataa tcatgatact
    19921 aagagctcca tggtaaacac cactagccct ttacctttct atcaattttt ctaaaataag
    19981 caaagaaacc cagttgacta caactgagtt catcttctaa gcaagagctt ctgaaatatc
    20041 tactgaattc gcctcatgcc atagcttctc tagattataa tgggcacgca tttcttctga
    20101 aaagatatgc acgacaacag caccgaggtc cagtaagacc cagcctccag ctgcatcgcc
    20161 ttcgacatgg ctacctttaa agcctgcttg agctactttt tcacggatat tagctgcgat
    20221 agcgtccaac tgacggctat tcattgagct agtgatgaca aagtagtccg tcacactagt
    20281 caaatcttgt acatcaagtg cgaggatatc ctccgcacgt ttctcatcag ccgctttcac
    20341 gactagttct agtaattctt tttcgttcat ttaatcctct ttttagaaaa tatgtttgta
    20401 atctaggaca tctgcaaagc gctcctccca aatgtcctca taaaattcat taatcgtttc
    20461 tgctggaatg tcatccccat caaaggttgt gacagcacct tctggaacaa taagctgata
    20521 gccatattca aaggcaacct tgacagaggt atccacacaa tattctgtct gcataccaca
    20581 taaaactaat ttttcaatcc cctgcttatc caagtattct tttaagccag tttctttgaa
    20641 aatactgtta tatttcttct gaaagacctt ttcagcaggt tttcgattta aaagcgcaga
    20701 taactgccaa tcttctgatg tttgagcttc agagttctca atatgttgaa catagataat
    20761 ttcaatattc ttgcttctag cctggttttg taaataagaa atttttccca atagactttt
    20821 tgtctggaac ccagtttcta ctaaaacatt ctgaacatca atgattataa aagccgtctt
    20881 catttagttc tctttcaaat agtgcacaaa ggcgttatag gtttcaaggg tttggggata
    20941 gatggggaat ccctgatgag ctagatactc cacggtacga gctgtttcgt aggccaccgc
    21001 cttattgagc gatagacttg caatctcacg cgccacatcc actccaggaa atgcccgatt
    21061 gtgctcgata tagtctgcga cgtagatgac cttatctagg tctgtcattt gaccagctcc
    21121 gactgtatga atttcaatgg ctcgcagaat ttcagaatca tgcaaatcca aatcttcctg
    21181 aattttgtaa attccgacca taccatgcca gacattattg ccccagtttt tgaggtcagg
    21241 gtctagctgg taacggtcaa tcaagtctag aaattcctga tctgacaact ttttagcata
    21301 gtcatgaaga agacctgcta gacttgcttt ctcaacatct acaccaaagc gctgagctaa
    21361 ttccatagca gcacgctcca cacctagaca atgggttaga cgtttctcag gtagaagctc
    21421 tgccattttt tccaacaagg cctcacggga gcagttgata tagtcttgat aggacatcag
    21481 tagagcccct ccttctcgat gtagtctagc actggctgag gtaggagaaa gttgggtttc
    21541 cgaccttggg caatgaagtc ccgcaccatg ctggacgaga tatccatgag cggtacgtcc
    21601 acccagataa ctggatagga agtccctgcc ttgtagcgtg gacgctgaac ccccacaaac
    21661 tgaaccatgt caaccagttc atcaattcgg taccacttag gcagatagtc aaccatgtcg
    21721 gcgccgatga taaagtaata atccgtatct ggatgttgct ctgtcaagat tttcatggtg
    21781 tcgtaggtgt aggaaatacc cttgcgctcc aactcaatgg tttcaatgac taggccgtca
    21841 atcccctcaa ttgccaactc aagcatcttg agacgatggt gttcagggat ggtttccttt
    21901 ttatcaacgt gaggaggttg gtattcaggc atgagaagaa cttgatccag tcccaactgt
    21961 tgccgtactt gatccgcaac aatgagatgg gcattgtgaa cagggttaaa attcccccct
    22021 aaaatcccaa cttgtttgcg ttttttctcc ttgatttctg gctccaactc taccttggta
    22081 aagggagtca atagttcgat tgtcataggc tagtcccctt tatttctcta taaaaacagt
    22141 tatttggagt ttcggtctta gatttctttg actttcttag aaatcttgcg attttctttc
    22201 ttgctagatt gtttaaacaa aatcaagatg cgtcctattt tttggactgt atccacaccg
    22261 atttcttctt ccaaaatttc agctacttcg tggatgtttt catctgtgtt ttgtaagaga
    22321 gtaaccttga ttaattcacg cgcatcaaga gcttgacgga cgctggtttt gatttggtcg
    22381 ttgagtccat ttttcccgat ttggatgata ggtttgaggg tgtgtgcctg gctgttgagg
    22441 aaggcacgtt gttttgatgt taatgacata attttcttcc ttgttttttg atagttattt
    22501 taggtggtga gggacggtcg gaactaattt tccaaagatt tcatctttga aaaatggatt
    22561 tcctgacctc acaattgagc cttggtctca attgtgcccc ttgccaatct acagattggc
    22621 aatcacacca cggcaataac tatcgctata tgagctcact ttgttcgctc agcgattttg
    22681 ttttaaataa ttgcttttcg tatgacgacg gcgacgcctt ctggtgccca gacggcgact
    22741 tttgctatgc ctgttacacg aatccagcct aggcctgaga tgactaggtc tgtcttatct
    22801 ttaatggtaa agacatgctg gactagcctt gggaaatctt ctttttcctt gctatttggt
    22861 ggtgtcagaa gagttcccag gtgcttatcg tagaaagcac tagctccttc aagcttgcta
    22921 cgatggagtt tgagttcatt atcaaagaaa gcagtaaatc cttgcttttc tcctgctata
    22981 aagtcaaagc gtcccaaacc acctaaaaat agggtttgct caggattaag ctgataggtc
    23041 ttaggcttga tttccttttt aggactgaca tacttgaggt ttttggccgt caagtagtga
    23101 gccatctggt ggcggtggat aattcccggc gtatcgtaaa tataagatcc gtcgtcaagc
    23161 ggaatctcta ttttgtccaa ggttgtccct gggaagcgtg aagtagtgat gacattctga
    23221 tcacccgtga tttcttggat aatagcattg attagagttg attttccaac gttggtcaca
    23281 ccgaccacat agacatcgcg gcccttacgg tagtgttcaa tcttgtcaat gacttcctta
    23341 atggcatgtt tattttgtgc tgaagttagg accacatcga ctggacgaag accttcttca
    23401 tgggcacgtt tcatgagcca ctggctaatc ttaccagact taactgactt aggaaggata
    23461 tcttttttat ttcctaccaa gaggacatca ttgcccgaga cgaaacgtgg taaacctggg
    23521 atgacagatc cattaaaatc aaagatatca atgacattga ccactaaagc atcactgtct
    23581 cccacctcgt gcaagagctt gaggaaatca tcgttcgtca actggacatc tgtgatttca
    23641 ttgtagtggc ggagacggaa acagcgttgg caatagactt cgccagtctc caaacctttt
    23701 tcaagtgccg actggggggt aaaaccaaga ccagctttat ctgtcgtctg aatggttgct
    23761 ccacaaccaa tacagagaat ttcttccata gttaaattcc ttttttatat gtaatcggtc
    23821 cgtacttttc agtgattttt ctcataacac gacgctcacg agttcggtta atctgcgttt
    23881 tgattgagtc atgttggacc aagggtttga ctaaaattga ccgaatccct gcacggtggg
    23941 ctgctcgtat atctgtcatg agctggtcac caaccatgac cacttccttt ttgtcatagt
    24001 ggaattcctt catagcacgg tcaataccaa atgtgaaggg cttcaaggcc cagtaaacgt
    24061 aatcaatccc aaatttctca actgctcgtt gaacgcgttt tttggtgtta tttgacacta
    24121 cgataatgcc aatacccgcg tcccgaaggt catgtagcca ttgcttcatc tctggcgttc
    24181 catcagggtt gttccaagca atgagggtat tatccaaatc gaccaaaaca gccttgatgc
    24241 cctgcgcctg caggcttggg actgtcagat catagactgc ttccacagca aaatctggta
    24301 tataattttc aatcgccatt ctggctcctt ttcttaactt tttttcgcaa ttttccttag
    24361 taacaaggat aaaacaatcc acaaacctgc actagccaaa ctgatataga gccaagcatg
    24421 gggctcatct gttaaaggta agggaacatt cattccgaaa aagcctgtca cgactgccaa
    24481 aacagctagc aagactgaaa tgatagtcaa ggttgtcaaa ttgtcattca gattattgtt
    24541 tagaatattg ttgtaagagg ctgaaagctg ctgtaaaatc tgagagatta ggtctgtcat
    24601 ggataccagc tgatgagcct caatcatggc atcatcaaac tgttctctct caatctcatc
    24661 aaaactacga tacaaggcat gaccttgaat atgctctaac aaaatccgat tttgtttggc
    24721 agctgccgtc agataaacca taccagtctc caaatcagac agggcaaaga ggtttttctt
    24781 ggtagttcgc tggcgcaaaa ggtcattgac ctcatcccta ctcttgtcca tctgctcaat
    24841 gacaggatag taggcattgc tgatgatttc cagactggca aagagaaact tataaatcga
    24901 aagcgtgtca tggctctcca gataacgagt catctgtcca atgacatagg cgttcttggt
    24961 attactaatg gtaatcaggc gacgatgctc gacgataaag gtcatgggaa aagtctcata
    25021 gtaggccttg tcctttttta cgtctaggac attatagata aaggtaaccg tcccactttc
    25081 acggtggtag tccatgtggg cgcgctcgtt tctatccagt gcgtattcaa tggtttcctt
    25141 gtccaaaccg taaatttcag aaaggtcttc tagtttattc aactttccta ggtctaggtc
    25201 tatccaggta caaccattgc ccaactgctt ttctaaaacc atcttttctc cttattaaac
    25261 tccctctatt gtaccacaaa ttacttaaaa taacaggtct agtctgtgcg tgagaaattg
    25321 ctaacaactg tgatatttcg gttagggaca aagtgaaggg cttgctcctc gctacgaagc
    25381 tgtgtcgtac gaattcccac actgacaacc ttacccgata cagtaatcgg tccatttgtc
    25441 agaacgacct catctcccac atccagttga cgttcaaaga ggataaaaaa gccattgatg
    25501 acatcagaca gaaagccttg ggctcccata ccaatcgcta ccccagcaat accagctcca
    25561 gccagcaaac tagaaactgg caaacctaaa atcgacaaaa tgcagtagag taaaaagaaa
    25621 tatagcgtat aattaaacac attttctagt aaacgtgaga tggttttttg gcgtccgaca
    25681 tcatgacgag acatttttag agaaggtttg acaattctct gcaccatggt atgaagcatt
    25741 tttttagcaa tataaaatac aatcaaaagc agtaaaagag aaatgacctt ggttagaata
    25801 ttctcgataa tggttgtcac atctagcttt tcaatataag cctgaataaa tttttgcata
    25861 taaaactcct ttatttcatt ataccatatt ttcatcagtc tcattctcca taaatattca
    25921 aaaaatagac agaaaattct tgattttgtc tatcatttat actataatca taatcactac
    25981 acaaaaagaa gattattatg aaaagaatca ttcatgcttg gaataaggca agccttgtca
    26041 agcgtatctt gattggtatg ctttatgggg aatcctagga ctgacccttc ctaatctctc
    26101 agggattggg ctactcgggg atttattcgt tggaggtcta aaagctgttg ctcctatcct
    26161 agtctttgcc ctcgttgcca atgccctttc ccaacatcaa aagggacaag atagcaatat
    26221 gaaaactgtt gttttcttgt atatcctatg atagggactt tcgccgctgc tcttgtagct
    26281 gtactagcaa gtttcatcgt ccctattgaa attaccctaa atagtgccaa tactgaaatt
    26341 gcaccaccag atgggattgg gcaggtcctc agcaacctct tgctcaaact ggttgacagc
    26401 ccagtcaacg ccctgcttac tgctaactat attggaatct tatcttgggc agtcattttt
    26461 ggaatcgcta tgagagaagc cagtaaaaat agtaaagaat tgctaaaaac tatcgctgac
    26521 gtgacttcta aaattgtcga atggatcatc aatctggctc catttggaat ccttggtctt
    26581 gtttttaaaa ccatttctga caagggagtc ggaagccttg ccaactacgg tattttattg
    26641 gttctattag taacgactat gctttttgtt gcccttgtgg tcaccctttg attgccttct
    26701 tctttatgag acgtaatcct taccctctag tttggaactg cctccgtgtc agcggtgtga
    26761 cagccttttt cactcgtagt tctgcggcta acattcctgt caacatgaaa ctctgccatg
    26821 accttggact caacccagat acctattctg tttctatccc actcggttct actatcaata
    26881 tggctggagt agcgattacc attaacgttt tgacccttgc tgcagttaac actcttggaa
    26941 ttcctgttga ctttgccaca gcctttgtcc tcagtgtggt agcagctatc tcagcctgtg
    27001 gtgcttcagg tattgccgga ggttccctcc ttcttatccc agttgcttgt agccttttcg
    27061 gtatttctaa cgatattgcc atacaagttg ttggggttgg ttttgtgatt ggtgtcatcc
    27121 aagactcatg tgaaacagcc cttaactctt ctacagatgt cctctttacc gccgttgccg
    27181 aatacgcagc aacccgtaaa aaataactca tcaaggcaag cctgctcatg tcttgtcttt
    27241 tacgctttta ttctaactta ttaggaaatt cttatgtcta ttagccaacg tacgaccaag
    27301 ctcatcttag ctacctgtct tgcctacctg cttgcttatt ttctcaatct ttcatcagca
    27361 gtttcggctg gaattatcgc tctcttgagc ctatctgata cgcgtagaag tactttaaaa
    27421 ctggctcgca atcgtctttt ttctatgctt ctagctctgg ctatcggtgt tctagctttt
    27481 cacttgagcg gatttcatat ctggagtctc ggcctctatc tggccttcta cgttccttta
    27541 gcctacaaga tgggctggga aattggcatc acaccaagca ctgttttggt tagccatctc
    27601 ttggttcaag agtcaacctc tccagacctt ctagtcaatg aattccttct ctttgctatt
    27661 ggtacaggat ttgccttgct tgttaatctc tatatgcctt cacgagaaga ggaaatccag
    27721 cactaccaca cgctggtgga agaaaagtta aaagatatcc tccagcgctt caaatactat
    27781 ttatccagag gagacggacg caaccgagca cagctggtag cagaattaga cacgcttttg
    27841 aaagaagccc tcagactggt ctatttggac cactctgacc acctctttca ccagaccgac
    27901 taccatatcc actactttga gatgagacag cgacaaagtc gtatcctgag aaacatggcc
    27961 caacagatta acacttgtca ccttgccgcc agtgaaagcc tgatcttagc gcaactcttt
    28021 tcaaaaattg caggtcaact gagccagacc aatcctgctt ctgatttgct agatgaaatt
    28081 gaacgttatc tggaagtctt ccggaaccgc agtctgccca agacaagaga agaatttgaa
    28141 acccgcgcca cccttcttca actcctacgt gaagccaaaa ccttcatcca agtaaaagtt
    28201 gatttttacc aaaaatatag acagtaaaaa agaaaaccac taaaaaacag agcttgccag
    28261 cattcttgaa aagtagtata attattgcat gcgcaatcat cttatgacgc agagactgcc
    28321 cgttaatgga ctttcttcta tttatgactc taaaaagaaa ggagatacta tgacctattt
    28381 ggaaaaatgg tttgacttca atcggcgtca gaaagaaatt gaaagtctct tggaagagac
    28441 cattgcccaa cagagcgagc aaagtctgac cttgaaagag ttctacctgc tctactatct
    28501 ggacttagct gaagaaaaat ctctacgcca gattgacctg ccagataagc ttcatctgag
    28561 cccgagcgca gtttctagga tggtggctcg cttggaagct aaaaattgcg gtctactcag
    28621 tcgcatgtgt tgccatcaag atagacgctc tagttttatc tgcctgacaa atgatgggca
    28681 aaagacactg gcctcgctac aaaagactgt cgaagaaagc ttggaagcag gtttggattt
    28741 cttgatttaa gatgatttta aaaaaagttg cgtgcgcaat catttttctt gacattctct
    28801 tttataagga gtaaaataaa gttagcatta aacaaaggag ttttaaatat gattgaaatt
    28861 acctatttag atgccagcaa gaacgaaaga actgtgactt tcgagtctta tgaagacttt
    28921 gaacgttcgc aacaagcttg ccttatcggc gtcgcagact actaccctgt ccaaaaatta
    28981 acttacaagg gtcataattt ggactaccat gggacttatg gagatatctt cttctatctc
    29041 atgaaacaag atttaagcca atataactaa aaaaaggaga aatacaatgg caaaagcaat
    29101 tacagatgca acattcgaac aagaaacaaa agacggtttg gtcttagtag acttctgggc
    29161 aacttggtgt ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt ctgaagaact
    29221 ttcagaagat gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa acacagctcg
    29281 tgcttttgga atcatgtcta ttccaactct tctcttcaaa aaagacggcc aagttgtcaa
    29341 acaagttgca ggtgttcaca cagcagaaca aatcaaggcc atcattgctg aattgagcta
    29401 atcaaacgag agaccaagta ctttctttgg tctctttttt cttgcccttt gccatttttc
    29461 aaaaaatatg ctagactata gtagattgaa actagaatag tacacctcta cttctaaaac
    29521 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttatt tcattttact
    29581 atataagtag aatattacga tgtttggggt atgccatcgc taaaaaaact cgatttggta
    29641 ttttttagtt ccctaatggg agctttttgc gtgctctgaa catttcccat tttggaagga
    29701 gtactgtgaa acgtcaatca gccttggtcg tctttagtgg tggtcaagat tcaacaacct
    29761 gccttttctg ggccatgcaa cactatgaaa cagtcgaagc tgtcaccttt gcctacggcc
    29821 aacatcatca cctcgaaatt caaattgcta gagaaatcgc caaggaacag ggcattcgtc
    29881 accatatcct cgatatgtct ctgctgggac aaatcactgc tcaaccagac tttgcgacga
    29941 ttcatatttc ctacattcct gacaagctct gtgtcgagtc aaaatccctc aaactatatc
    30001 tatttagcta ccgaaaccac ggagatttcc acgaaaactg tatcaacacc atcgggaaag
    30061 acttggtcaa cttgctagac cctcgctatt tagaagtctg gggaaaattc actccgcgcg
    30121 gtggcatttc aatcgacccc tactataact acggtaagca aggaactaag tatgagggct
    30181 tggcagaaca acgcctcttc caacacgacc tttatccaga gaaaattgac aaccgctaaa
    30241 ctcatactca atgaaaatca aagagcaaac taggaagtta gccgcaggtt gctcaaagca
    30301 gtgtttgagg ttatagataa gactgacgaa gtcagcttaa aacactgttt tgaggttgca
    30361 gatagaactg atgaagtcag cttaaaacac tgttttgagg ttgtagatga aactgacgaa
    30421 gtcagcttaa aacactgttt tgaggttgca gatagaactg acgaagtcag cttaaaacac
    30481 tgttttgagg ttgcagatag aactgacgaa gtcagcttaa aacactgttt tgaggttgca
    30541 gatagaactg acgaagtcag cttaaaacac tgttttgagg ttgtagatga aactgacgaa
    30601 gtcagcttaa aacactgttt tgaggttgta gatgaaactg acgaagtcag cttaaaacac
    30661 tgttttgagg ttgcagatag aactgacgaa gtcagtaaca tctatacgac aaggtgaagc
    30721 taacgtggtt tgaagagatt ttcgaagagt atcatacgaa aaagccctgt tcctcaagat
    30781 gaggagcaag gctttttgag tttcaattat tcttctgttc caaggaaatt ttttgcaaca
    30841 agggctgcaa gaactccacc agcgattggt gcaaggatga aaatccaaac ttgttgaagg
    30901 gctgcgccgc ctaccaagac agctggtgcc aagctacgag ctgggtttac tgaaagtcca
    30961 gtaatcttca atccgacaag aatcatcgcc atcaatgaca aaccgattac caaaccagca
    31021 atcgcgccat tgcccttgct ttctgaagtc acagtcatga taaccaatac aaacaagaaa
    31081 gttgcgatga cttcaaacaa gaaaccacca aagacagtga caccgtttgc caaggcattt
    31141 tcaccaagac tagcagttga catacctgag ttagccaaga ggaagaagac agcgccagaa
    31201 gcgatgaaag ctccaacaac ctgaccaagg atgtagttta caagttctga agatgacaaa
    31261 cgtttgttta caaacatagc aatcgaaaca gccgggttca agtgagcacc tgaaacagtt
    31321 ccgattgagt aggctgccac cacgattgcc aaaccaaagg caaaggcgat tccaaggtaa
    31381 ccaaggccat caagaccatt tccaaaaaca acagctcctg tcccgacgaa cacaagcatg
    31441 aacgtaccga ttaactcagc aacaaatttt ttcattttct ttctcctttt ttcaaaaact
    31501 agatactagt ctatcaaaag taggaaaggg tttcaagaaa attgattgga aattttttga
    31561 aaatcataga actattagct aatctctagt attgaaaaga ctggatagct tctttcaact
    31621 catcttgtaa actatttctc tggtcaagtt ggacatagac ttccaccaga caggatctga
    31681 agttggaaaa tttgtaaaaa tcctcccttt cttctatcgg aaaatcaaca gtttttatcc
    31741 aagaagctac ttgttcttgc tccaacttcc cttataaaat aggttcatag atcactcttg
    31801 ctaaacgcca atcctcatca tctgtaaagc gaatcgacat tcttttaaat agttggccaa
    31861 gtatatcaaa tacttcatga actctgtttt taggaaagtc tggatgacaa accacctctg
    31921 tcagtaaatc ggctccatgt gcaaaagcgt gaacccaacc atactgactt gagaaacctg
    31981 ttgtatcctt ttcttttgaa agatagtgca agccttgatt tgaaaggtca tcacgaattt
    32041 ctggagaagg atttcccaaa tgatcaaaca accactggat ttcttcctgg ttataatttg
    32101 gtttttcttc tgctattttt cttagtaaat cttgatacat ggtcaatacc tctacatttc
    32161 tagcaactgt tcaaaaaggc agtcttaaat gactcaatat tgaattctca attaaataca
    32221 atctgatata aaatgacgta aataactatc aataccagtt ctacagtaag ttcaaattta
    32281 acatcacgac cttcaacgac atttttgaaa atagctacaa ctaagacaaa tagaatgacg
    32341 cttaacaagc ccataaacat cattctaaaa aatttttcta tccccttact cccccaactc
    32401 agcactgtag gcgataatct ggtcaactgt gtcagacaag aattggatgg tatcacggag
    32461 tggtttgtct gttgaaatat ccgcaccgat aatcatggct gactcaagtg gtgtcttgct
    32521 accacctgat ttgaggagat tgagccagtc ttcagctcca gtttctgagt ttttaagatg
    32581 aaggtaacca gcagtcgaga taactagtcc tgctgagtaa gtgtaactat acaagcccat
    32641 atagtagtga gcttggcgca tccaagtcaa agctgcatcg tcgtcgattt caatggcatc
    32701 gccccaaaaa tctgtcaaaa cttccttcat aatgctgttg agcttgcttg ctccaaaggt
    32761 ctccccttct tcaatcaatg tatacacctt acgctggaag gcggcttcca agaggtgggt
    32821 gatgaaatta tggaagtagg tgtctgtcaa gcgatgagcc agagcgaagc gtttttgacg
    32881 tgggtcatta gactggttct ctaagtaatc actgagtagc aattcattga aggtcgacgg
    32941 tgcttcaaca taataggtcg acatgtgggc gttgaaatag ctttgatgat tgtctgaaaa
    33001 gatgaattga ccagaatgcc cgatttcatg aatcaaggta tagacatcgc tcaaacggcc
    33061 tgtccagctc atgagtacat aagggtgtac gcgatatggg tccgccgcat aaccaccgga
    33121 atccttgcca ctgttagcag caaagtccac ccagcgctct tcttggtaac gagcaacttc
    33181 ctgacaatat tcttgcccca aaggttctac cgacttcatg accaaatcat aggcatcgtc
    33241 aatagtcact tcaggattca gggcgctgtc caagtccaat ttccaatctg caaaggtcat
    33301 cttttcaaga ccatttacct tggcaacatg cttgaggtat ctctgagcga ctggtgcaaa
    33361 gtccttcatg atgaggtcaa tctggcggtc aaacatgaca cggtccactt cttgttcagc
    33421 tagaagataa tcaaagacag agtcgtatcc cttcatatca gccaagagtt tttcagactt
    33481 gacctgagcc agataggctg ctgcagccgt attttggtgc ttacgaagtc cctctgagaa
    33541 ggaacggaag gatttctcac gaacctcagc atcctcgtga ttttggtaga aattctcata
    33601 ggtgacaaag ctgtttttat aggtcttgcc atgggcttca aagtcagcca tttcaaaatc
    33661 cccagctcgc atcttagtat aaatgtcctg cggactatag aaaacttcac cgagattggt
    33721 caaggccttc tccacatcag cccctagata gtgggctttt ttgattttgg cctgacgaat
    33781 agctgccgtc aagtgaggca attcacccaa acggtccaag acttcctcgt ctgctaccac
    33841 caaggcatcg tcaaagaagg tcaaggctac gctggcatct gtttcaaatt ccatcccagc
    33901 ttgggcaata ttggcaaatt cgtcattgct atagtccgtc gtctgaggca taaaagcata
    33961 gttgccaata tggctcatct gaatgtagat ctgttccaat tccgcaaagg ccttctcgaa
    34021 atcctcaaaa gtgtgaagat tgcccttgta atcacggcta aattggttga tgtcttcacg
    34081 agctttctcg attgcacgca agaaatcctc acggtcttgg tatagggctg ttaagtccca
    34141 gagttccttc tctggaaatt ctgaacggtg tttttgttcc attttcttcc tcttatttct
    34201 ctaattctac taaaacacta agggctgata aggcgtaaag cggtgctgtt tctgctcgca
    34261 aaatacgagg acctaggcct gccaaaactg ctcctttagc ttcaaaactt tcgatttctg
    34321 caggtgagag accaccttct ggaccaaaga taaagagcag tttggctcct gtttcaagac
    34381 cagtgactgc ttgcagaagc gcagcggctt ctccttcttt agctgattct tcataggcta
    34441 ctatgataga gtcaaactgg tccagctgag ctagaaaatc tgcttttttc tcgaaaagtt
    34501 taatacttgg tacaatatta cgcttgcttt gctcggctgc tccaagggca attttttcta
    34561 gtttttcaac ttttttaccc aatttcttgc catcccactt ggcaactgac cagtctgcag
    34621 gaaaggccca gatttggcta gcacccagtt cggttacttt ttgagcgatg aactccagct
    34681 tgtctccctt gggaaatcca gatgcgatgg tcacttggac tggtagttcc acattgtcat
    34741 ttaattcttg gaccaactca aactgacgat tttccatatc cagcacgcgc gccaagcgct
    34801 tgatgccatc atcaaagact aaggtaacct catcctcttc tttcaagcgc ataacctgaa
    34861 acatatgctt actgatttcc ttgtcctcga tagtgacagg agagatagca ctgcctttta
    34921 caaaatactg ctgcatgcta gcctccaatc acccctgaaa tatccttggt cttcttaaag
    34981 acacaggcat tccactcccc ttgaatcatg tgggtttcga ggaaaaatcc agccgattca
    35041 gccgactcac gcaccatgtc ccacttatcc ttgataatgc cactcatgat caggtagcct
    35101 tcgtccttga ccaaacgata ggcatcgtct attagatgaa tgaggatatc cgccaagata
    35161 ttagccacaa tcacatctgc ctcaatttcc acacccttaa gcaaatctcc agccgctaca
    35221 tggatatttt ccatgccagg gttgagctca atattttcct gagccacacg aaccgccaca
    35281 tcatccaggt cataggcgaa aatttcttta gcccccagaa gcgagctggc aatagagaga
    35341 acccctgaac cagtccccac atctagcacc gtttcgccac cacgaagaac ctgttccaag
    35401 gcaaaaaggc tcatcttggt agttgggtgg gttccagtac caaaagccat gccaggatcc
    35461 agcttgataa ttttttctcc cgcagtcgcc tcatagtctg tccaagaggg aacaatggtc
    35521 aaatcatgag tgatacgagc tggctcatag tatttcttcc agttgtctgc ccagtcttcc
    35581 tcagccaagg cagtcgtacc tatttttaac tctcccaaat ccataaaatc tgtcaattct
    35641 gctagacgag cctgcaaatc cgcctcaacc actgtcacat ccaccgtgtc agggtagtag
    35701 gcggtcacta cgatttcttc ttgctgctcc acctctggga aaatctcgcc aaagcggtca
    35761 acatttccca catagtccag actgtcttca atcgcaacac cttgcgctcc cagctcaatc
    35821 aagagattgg aaaccaactc ctccccctca cgcttcactg taacttttaa ctcttgccat
    35881 gtttccattt tgttttttaa aattacctct ctattagatc aaaaatagtc ttaccaataa
    35941 cgacaataag gttgtctctt aattttttct ctaactctaa gtctatacca ctattctgta
    36001 tgaatttatc ttctacattt ctatataggc gttttgctct aatgggattc cccggatcaa
    36061 aatgaggtac atagtggtaa attatgacag gaacagaacc gctgattgat cgttcagcaa
    36121 tctgactcca gatataccta tcagtttctc ccaaagaaac cccatagaga ataatgatgt
    36181 cagaattttt tataatatca ctattcttta catccatatt ttctctatag ttttttaaaa
    36241 ctaaactttt tattagggat tccttttgtt cttctaaact atttgaaatt tgtatttcat
    36301 cactaacccc taaaaccgtg cagacatcca ccgtcccatg tgcatgaatt ggtggctcaa
    36361 tataaaaatt ataaagatta gctcgtaatt gattcctgaa tgattttgct gacccattat
    36421 ataatttatc gattacatgt gtataattaa gtgttactat atttacaatg tcgtcctgat
    36481 gtggattctc attgtatttt ttatcgatag caggttgatt aattacaggg agagaggtcc
    36541 tcatatcatc tagagtgctt ataaagtcaa ttgcattacc tttctcaaga tttttttctt
    36601 gttgtatgcg tagata
//
LOCUS       DAVHIU010000007        34261 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVHIU010000007 DAVHIU010000000
VERSION     DAVHIU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.007"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            440..892
                     /locus_tag="VVN06_000283"
     CDS             440..892
                     /locus_tag="VVN06_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU5561894.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            911..2248
                     /locus_tag="VVN06_000284"
     CDS             911..2248
                     /locus_tag="VVN06_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU5561895.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIGVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2327..3211
                     /locus_tag="VVN06_000285"
     CDS             2327..3211
                     /locus_tag="VVN06_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5561896.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKVVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3211..4044
                     /locus_tag="VVN06_000286"
     CDS             3211..4044
                     /locus_tag="VVN06_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5561897.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4062..6155
                     /gene="nanB"
                     /locus_tag="VVN06_000287"
     CDS             4062..6155
                     /gene="nanB"
                     /locus_tag="VVN06_000287"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU5561898.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGMEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDACYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6168..7271
                     /locus_tag="VVN06_000288"
     CDS             6168..7271
                     /locus_tag="VVN06_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5561899.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQNGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVVHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7445..8143
                     /locus_tag="VVN06_000289"
     CDS             7445..8143
                     /locus_tag="VVN06_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU5561900.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8162..9679
                     /locus_tag="VVN06_000290"
     CDS             8162..9679
                     /locus_tag="VVN06_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5561901.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9804..11132
                     /locus_tag="VVN06_000291"
     CDS             9804..11132
                     /locus_tag="VVN06_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5561902.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11385..12251
                     /locus_tag="VVN06_000292"
     CDS             11385..12251
                     /locus_tag="VVN06_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5561903.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12267..13106
                     /locus_tag="VVN06_000293"
     CDS             12267..13106
                     /locus_tag="VVN06_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5561904.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13379..13831
                     /locus_tag="VVN06_000294"
     CDS             13379..13831
                     /locus_tag="VVN06_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU5561905.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            13973..14281
                     /locus_tag="VVN06_000295"
     CDS             13973..14281
                     /locus_tag="VVN06_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561906.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14568..15212
                     /locus_tag="VVN06_000296"
     CDS             14568..15212
                     /locus_tag="VVN06_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU5561907.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15381..16298
                     /locus_tag="VVN06_000297"
     CDS             15381..16298
                     /locus_tag="VVN06_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5561908.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16316..17200
                     /locus_tag="VVN06_000298"
     CDS             16316..17200
                     /locus_tag="VVN06_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5561909.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17216..18067)
                     /locus_tag="VVN06_000299"
     CDS             complement(17216..18067)
                     /locus_tag="VVN06_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU5561910.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18267..20324
                     /gene="pbp2b"
                     /locus_tag="VVN06_000300"
     CDS             18267..20324
                     /gene="pbp2b"
                     /locus_tag="VVN06_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU5561911.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKILETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGELTPDSLGTVTNVFIPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPYESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVSHGTSPLTTGRAFSDGATVSISGKTGTG
                     ESYVAGGQEANNTNAVAYAPTENPQIAVAVVFPHNTNLTKNVGPAIARDIINLYNQHH
                     PMN"
     gene            20335..20931
                     /gene="recR"
                     /locus_tag="VVN06_000301"
     CDS             20335..20931
                     /gene="recR"
                     /locus_tag="VVN06_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU5561912.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMPDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDEDPCSICTDPSRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21107..22150
                     /locus_tag="VVN06_000302"
     CDS             21107..22150
                     /locus_tag="VVN06_000302"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU5561913.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMSAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSQTPGQEDRLMTNATIDWDKKVVPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLTYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTKWSMYPLLWDNMGISYPDLIERLVNL
                     AKESFDKREAHLL"
     gene            22235..23608
                     /locus_tag="VVN06_000303"
     CDS             22235..23608
                     /locus_tag="VVN06_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5561914.1"
                     /translation="MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKELVNHPYILVDDVLTAFQTLAAYYLEKTGVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELAHPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMPEGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLIERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQNLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDAVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23595..24206
                     /locus_tag="VVN06_000304"
     CDS             23595..24206
                     /locus_tag="VVN06_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5561915.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQGSDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24297..25001
                     /locus_tag="VVN06_000305"
     CDS             24297..25001
                     /locus_tag="VVN06_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU5561916.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25220..26593
                     /gene="ftsA"
                     /locus_tag="VVN06_000306"
     CDS             25220..26593
                     /gene="ftsA"
                     /locus_tag="VVN06_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5561917.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26610..27869
                     /gene="ftsZ"
                     /locus_tag="VVN06_000307"
     CDS             26610..27869
                     /gene="ftsZ"
                     /locus_tag="VVN06_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5561918.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27874..28545
                     /locus_tag="VVN06_000308"
     CDS             27874..28545
                     /locus_tag="VVN06_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5561919.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28555..29094
                     /locus_tag="VVN06_000309"
     CDS             28555..29094
                     /locus_tag="VVN06_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU5561920.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29094..29357
                     /locus_tag="VVN06_000310"
     CDS             29094..29357
                     /locus_tag="VVN06_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5561921.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29354..30139
                     /locus_tag="VVN06_000311"
     CDS             29354..30139
                     /locus_tag="VVN06_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU5561922.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGIACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            30148..30936
                     /gene="divIVA"
                     /locus_tag="VVN06_000312"
     CDS             30148..30936
                     /gene="divIVA"
                     /locus_tag="VVN06_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU5561923.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31284..34076
                     /locus_tag="VVN06_000313"
     CDS             31284..34076
                     /locus_tag="VVN06_000313"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5561924.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTSANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPTPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10527 a         6094 c         7454 g        10186 t
ORIGIN      
        1 attattttag aaaaacgaag tgagcagctt ataaattcaa gtccccaaat agattcatac
       61 tagtatcttt tgcaaaaaat aaagggcgac ttccgtcatg aatatcaatt tcatctataa
      121 ggaaggtagc taattgaact aacttattta ttctgtttgt cgctagaaaa atcagacctc
      181 cttgtgaaga ttgaggagat actgaatgaa aatcaaagaa gaaactagca agctagtagc
      241 agattgccca aaacaccgcc ttgaggttgt agataagact gacctatata atccaaggtg
      301 aagcgactgt ggtttgaaga gattttcaaa gagtataggc tagagagtag tgtttttatg
      361 tccttctagt agaaaatgct agacagaaga atggggaact tggataggaa aaatagattg
      421 agaaaggagg ttagaagaga tgattattac aaaaattagc cgtttaggaa cttatgtggg
      481 agtaaatcca cattttgcaa cattaataga ttttctagaa aaaacaggac tagaaaattt
      541 aacagaaggt tcgattgcta tcgatggtaa tcgattgttt gggaattgct ttacttatct
      601 agcagatggt caagcagggg ctttctttga aacacaccaa aaatatttgg atattcattt
      661 agttttggaa aacgaagaag ccatggctgt tacatcgccg gaaaatgtaa gcgttaccca
      721 agaatatgat gaagagaaag atattgaatt atacacaggg aaagtggaac agttggttca
      781 tttgagagct ggcgaatgcc tcatcacttt tccagaagat ttacatcaac ccaaggttcg
      841 tataaatgat gaacctgtga aaaaagttgt ctttaaagtt gcgatttctt aatgtagaaa
      901 gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta gctggaattg cgctagcggc
      961 tgttgccttg gtagcttgtt caggaaaaaa agaagctaca actagtactg aaccaccaac
     1021 agaattatct ggtgagatta caatgtggca ctcctttact caaggacccc gtttagaaag
     1081 tattcaaaaa tcagcagatg ctttcatgca aaagcatcca aaaacgaaaa tcaagattga
     1141 aacattttct tggaatgact tctatactaa atggactaca ggtttagcaa atggaaatgt
     1201 gccagatatc agtacagctc ttcctaacca agtaatggaa atggtcaact cagatgcttt
     1261 ggttccgcta aatgattcta tcaagcgtat tggacaagat aaatttaacg aaactgcctt
     1321 aaatgaagca aaaatcggag atgattacta ctctgttcct ctttattcac atgcacaagt
     1381 catgtgggtt agaacagatt tgttaaaaga acataatatt ggggttccta aaacttggga
     1441 tcaactctat gaagcttcta aaaaattgaa agaagctgga gtttatggct tgtctgttcc
     1501 gtttggaaca aatgacttaa tggcaacacg tttcttgaac ttctacgtac gtagtggtgg
     1561 aggaagcctc ttaacaaaag atcttaaagc agacttgaca agccaacttg ctcaagatgg
     1621 tattaaatac tgggttaaat tgtataaaga aatctcacct caagattctt tgaattttaa
     1681 tgtccttcaa caagctacct tgttctatca aggaaaaaca gcatttgact ttaactctgg
     1741 cttccatatc ggaggaatta atgccaacag tcctcaattg attgattcga ttgatgctta
     1801 tcctattcca aaaatcaaag agtctgataa agaccaagga attgaaacct caaacattcc
     1861 aatggttgtt tggaaaaatt caaaacatcc agaagttgct aaagcattct tagaagcact
     1921 ttataatgaa gaagactacg ttaaattcct tgattcaact ccagtaggta tgttgccaac
     1981 tattaagggg attagcgatt ctgcagccta taaagaaaat gaaactcgta agaagtttaa
     2041 acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt actgctattg gttatgaaaa
     2101 tggaccaagt gtacaagctg gtatgttgac taaccaacac attattgaac aaatgttcca
     2161 agatatcatt acaaatggaa cagatcctat gaaagcagca aaagaagcag aaaaacaatt
     2221 aaacgattta tttgaggctg ttcagtagat gtaaaagact agaaaatagg tgggctagtg
     2281 agctgaaaag ctctagccca atcttgtaaa agaagggaga aggagaatgg ttaaagaacg
     2341 taatttaact cgctggatat ttgttttgcc agctatgatt atcgtaggat tactctttgt
     2401 ttatccgttt ttctcgagta ttttttatag ctttaccaat aagcatttga ttatgcctaa
     2461 ttataaattt gttggtttgg ctaactataa agttgtgcta tcagatccca acttctttaa
     2521 tgcgttcttt aattcaatta agtggaccgt tttctcatta gttggtcaag ttttagtagg
     2581 gtttgtattg gctttagctc ttcacagagt acgccacttc aagaaattat ataggacctt
     2641 attgattgtt ccttgggcat ttcctaccat cgtcattgcc ttttcttggc agtggattct
     2701 aaacggggtt tatggctact tacctaatct aatcgtaaaa ttaggtttaa tggaacatac
     2761 acctgcattt ttgacagata gtacatgggc attcctatgt ttggtgttta tcaacatttg
     2821 gtttggagca ccaatgatta tggttaatgt gctttcagct ttgcaaacag taccagaaga
     2881 acaatttgag gctgctaaga tagatggtgc ttcaagttgg caggtgttca agtttatcgt
     2941 ctttccacat attaaagtgg ttgtaggact tctagttgtt ttgagaactg tatggatctt
     3001 taataacttt gacattatct acctcattac tggtggtgga ccagccaatg ctacaacgac
     3061 gcttccaatt tttgcttaca acctgggctg gggaactaaa ttgttgggtc gtgcttcagc
     3121 agttacagta ttgctcttta tcttcttggt ggcgatttgc tttatctact ttgctatcat
     3181 cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga aatccagtat ttatttagat
     3241 attctctcac atgtactttt agttggtgcg accatcgttg caattttccc attggtatgg
     3301 attatcatat cttctgtcaa agggaaaggg gaattaactc agtatccaac acgattttgg
     3361 cctgaacagt ttacattaga ttatttcact catgttatca acgatttgca cttcattgat
     3421 aacattcgaa acagtttaat cattgccttg gctacaaccc tcattgcgat tattatttct
     3481 gctatggcag cctatggtat tgttcgattc tttcctaaat tgggagcaat catgtcgaga
     3541 ctactcgtca ttacctacat tttcccacca attttgttag caattcccta ttcaattgcc
     3601 attgctaaag ttgggttaac aaatagttta tttggcttga tgatggttta tctatctttt
     3661 agtgttccat atgcagtttg gctcttagtt ggatttttcc aaacagttcc aattggaatt
     3721 gaagaagcgg ctagaattga tggtgcaaat aaatttgtta cgttttataa agttgtgcta
     3781 ccgattgtag caccaggtat tgtagcaaca gctatttata catttatcaa tgcttggaat
     3841 gaattcctgt atgccttgat tttgattaac aatacaggaa agatgacagt agcagtagcc
     3901 cttcgttcac ttaatggttc agaaatacta gactggggag atatgatggc agcgtctgtt
     3961 attgtagttc ttccatcaat tattttcttc tctatcatcc aaaataagat tgcaagtgga
     4021 ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa aatgaataaa agaggtcttt
     4081 attcaaaact aggaatttct gttgtaggca ttagtctttt aatgggagtc cccactttga
     4141 ttcatgcgaa tgaattaaac tatggtcaac tgtccatatc tcctattttt caaggaggct
     4201 catatcaact gaacaataag agtatagata tcagcccttt gttattagat aaattgtctg
     4261 gagagagtca gacagtagta atgaaattta aagcagatga accaaactct cttcaagctt
     4321 tgtttggcct atctaatagt aaagcaggct ttaaaaataa ttacttttca attttcatga
     4381 gagattctgg tgagataggt gtagaaataa gagacgccca aaagggaata aattatttat
     4441 tttccagacc agcttcatta tggggaaaac ataaaggaca ggcagttgaa aatacactag
     4501 tatttgtatc tgattctaaa gataaaacat acacaatgta tgttaatgga atggaagtgt
     4561 tctctgaaac agttgataca tttttgccaa tttcaaatat aaatggtata gataaggcaa
     4621 cactaggagc tgttaatcgt gaaggtaagg aacattacct cgcaaaagga agtattggtg
     4681 aaatcagtct atttaacaaa gcaattagtg atcaggaagt ttcaactatt cccttgtcaa
     4741 atccatttca gttaattttc caatcaggag attctactca agctaactat tttagaatac
     4801 cgacactata tacattaagt agtggaagag ttctatcaag tattgatgca tgttatggtg
     4861 ggactcatga ttctaaaagt aagattaata ttgccacttc ttatagtgat gataatggga
     4921 aaacgtggag tgagccaatt tttgctatga agtttaatga ctatgaggag cagttagttt
     4981 actggccacg agataataaa ttaaagaata gtcaaattag tggaagtgct tcattcatag
     5041 attcatccat tgttgaagat aaaaaatctg ggaaaacgat attactagct gatgttatgc
     5101 ctgcgggtat tggaaataat aatgcaaata aagccgactc aggttttaaa gaaataaatg
     5161 gtcattatta tttaaaacta aagaagaatg gagataacga tttccgttat acagttagag
     5221 aaaatggtgt cgtttatgat gaaacaacta ataaacctac aaattatact ataaatgata
     5281 agtatgaagt tttggaggga ggaaagtctt taacagtcga acaatattcg gttgattttg
     5341 atagtggctc tttaagagaa aaacataatg gaaaacaggt ccctatgaat gttttctaca
     5401 aagattcgtt atttaaagtg actcctacta attatatagc aatgacaact agtcagaata
     5461 gaggagagag ttgggaacaa tttaagttgt tgcctccgtt cttaggagaa aaacataatg
     5521 gaacttactt atgtcccgga caaggtttag cattaaaatc aagtaacaga ttgatttttg
     5581 caacatatac tagtggagaa ctaacctatc tcatttctga tgatagtggt caaacatgga
     5641 agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc agaagcacaa atggttgaac
     5701 tgagagatgg tgtgattaga acattcttta gaaccactac aggtaagata gcttatatga
     5761 ctagtagaga ttctggagaa acatggtcgg aagtttcgta tattgatgga atccaacaaa
     5821 cttcatatgg cacacaagta tctgcaatta aatactctca attaattgat ggaaaagaag
     5881 cagtcatttt gagtacacca aattctagaa gtggccgtaa gggaggtcaa ttagttgtcg
     5941 gtttagtcaa taaagaagat gatagtattg attggaaata ccactatgat attgatttgc
     6001 cttcgtatgg ttatgcctat tctgcgatta cagaattgcc aaatcatcac ataggtgtac
     6061 tgtttgaaaa atatgattcg tggtcgagaa atgaattgca tttaagcaat gtagttcagt
     6121 atatagattt ggaaattaat gatttaacaa aataaaggag aaaaaacatg gttaaatacg
     6181 gtgttgttgg agcagggtat tttggagctg aattggctcg ctatatgcaa aagaatgatg
     6241 gagcagagat tactcttctc tatgatccag ataatgcaga ggcgattgca gaagaattgg
     6301 gagcaaaagt agcaagttcc ttagatgagt tggtttctag cgatgaagta gattgtgtta
     6361 tcgtcgcaac tccaaataat cttcataagg aaccggttat taaggctgca cagaatggta
     6421 aaaatgtttt ctgtgaaaaa ccaattgcgc tttcttatca agattgtcgc gagatggtag
     6481 atgcatgtaa agaaaacaat gtaaccttta tggcaggaca tattatgaat ttctttaatg
     6541 gtgttcatca tgcaaaagaa ctcattaatc aaggagttat cggagacgtt ctatattgtc
     6601 atacagctcg taatggttgg gaagaacaac aaccgtcagt atcatggaaa aaaattcgtg
     6661 aaaaatcagg tggtcacttg tatcaccaca tccatgaatt ggattgcgtt caattcctta
     6721 tggggggcat gcctgaaact gtaaccatga caggtggaaa tgtggtccat gaaggtgaac
     6781 atttcggtga tgaagatgat atgatttttg tcaatatgga attttctaat aagcgttttg
     6841 ccttgttaga atggggttca gcttatcgtt ggggtgaaca ttatgtctta atccaaggaa
     6901 gcaaaggtgc catccgctta gacttattca actgtaaagg aactcttaag ctagatgggc
     6961 aagaaagcta tttcttgatt cacgaatcgc aagaagaaga tgatgatcgg actcgtatct
     7021 atcatagtac agagatggat ggagcaattg cttatggtaa accaggtaaa cgtactccat
     7081 tatggctatc atctgtcatt gataaagaaa tgcgctatct gcatgagatt atggaaggag
     7141 ctccagtatc agaagaattt gcaaaacttt tgacaggtga agctgcccta gaagcaattg
     7201 ctactgcaga tgcttgtacc cagtctatgt ttgaagatcg caaagtaaaa ttgtcagaaa
     7261 ttgtaaaata aattttggta ttctcctatt tataggtcga cttgctcctc tgaaagtact
     7321 tttagaggag ctgtttgact ttgctagttt ttgaaactga aatctattat actacaaact
     7381 attgaaagcg ttttaatttt aaggtataat aatctcatag aaataaagaa aaggaggaaa
     7441 gaggatgcca cagattagca aagaagcctt gattgagcaa atcaaagatg gaatcatcgt
     7501 ttcttgtcag gctcttcctc atgaaccgct ttatacagaa gcgggagggg tgattccctt
     7561 gctggtcaaa gcggctgagc aaggtggggc agtcggtatc cgagcaaaca gtgttcgcga
     7621 tatcaaggaa attaaggaag tcactaaact tccaatcatt gggattatca aacgtgatta
     7681 tccacctcag gaacccttca tcacggctac tatgaaagaa gttgatgaat tggcagaact
     7741 ggacatcgag gtgattgctc tggattgtac caagcgtgaa cgctacgatg gtttggaaat
     7801 tcaagagttc attcgtcagg ttaaggagaa atatcctaat cagcttttga tggctgatac
     7861 tagtatcttc gaagaagggc tagcagctgt agaagcagga attgactttg tcggaacaac
     7921 cttatcaggc tacacatcct acagtccaaa agtagacggt ccagattttg aattgattaa
     7981 gaaactctgt gatgccggtg tagatgtcat tgcagaagga aaaattcata caccagaaca
     8041 agccaaacaa atccttgaat atggagtgcg aggcatcgtt gttggtggcg ctattactag
     8101 accaaaagag attacagaac gcttcgttgc tagtcttaaa taagatgtga gggggagttt
     8161 tatgtttaaa gttttacaaa aagttggaaa agcttttatg ttacctatag ctatacttcc
     8221 tgcagcaggt ctacttttgg ggattggtgg tgcactttca aacccaacca cgatagcaac
     8281 ttatccaata ctagacaata gtatttttca atcgatattc caagtaatga gctctgcagg
     8341 agaggttgta ttcagtaatt tgtcactact tctctgtgtg ggattatgta ttggcttagc
     8401 gaaacgagat aaaggaaccg ctgcgttagc aggagtaact ggttacttag ttatgactgc
     8461 aacgatcaaa gctttggtaa aactttttat ggcagaagga tctgcaattg atactggagt
     8521 tattggagca ttagttgtcg gaatagttgc cgtatatttg cacaaccgat ataacaatat
     8581 tcaattacct tccgctttag gattctttgg aggttcacgc ttcgttccta ttgttacatc
     8641 gttctcttct atcttgattg gctttgtctt ctttgttatt tggccacctt tccaacaact
     8701 tcttgtttct acaggtggat atatttctca ggcgggtcca attggaactt ttctatatgg
     8761 atttttaatg agactttctg gagcagtagg cttacatcat ataatttacc ctatgttttg
     8821 gtatactgaa cttggtggtg ttgaaactat tgcaggacaa acagtggttg gagctcaaaa
     8881 aatatttttt gctcaattag ccgatccggc ccattctgga ttatttacag aaggaacaag
     8941 gttttttgca ggtcgtttct caacaatgat gttcggttta ccggctgcct gtttagcgat
     9001 gtaccatagt gttcctaaaa atcgtcgtaa aaaatacgcg ggtttgtttt ttggagttgc
     9061 tttaacatct tttattaccg gtattacaga accaattgaa tttatgtttc tattcgtcag
     9121 tccggttcta tatgttgttc acgcattcct tgatggtgtt agcttcttta ttgcagacgt
     9181 cttaaatatt tcaataggaa acacattttc aggaggtgta atcgatttca cattatttgg
     9241 aattttgcag gggaacgcta agacgaattg ggttcttcag attccaattg gacttatttg
     9301 gagtgttttg tattatatta tttttagatg gttcattact caattcaacg ttctaacgcc
     9361 aggacgagga gaagaagtag attctaaaga aatttctgaa tccgcagatt caacttcaaa
     9421 tactgcagat tatttaaaac aggatagcct acaaattatc agagccttgg gtggatcaaa
     9481 taatatagaa gatgtagatg cttgtgtgac acgtttacgt gtagctgtaa aagaagttaa
     9541 tcaagttgat aaagcacttt taaaacaaat tggtgcagtt gatgtcttag aagtgaaggg
     9601 tggcattcaa gcaatctatg gagcaaaagc aatcttatat aaaaatagta ttaatgaaat
     9661 tttaggtgta gatgattaag tacttactga cttaataaaa aacagaggag agtgatggat
     9721 gagtaggatg aaatgaaatc gcatacaaga aataaagaac tcattatcca agttggatac
     9781 gcttattaca taggagaata caaatgaaat ttagaaaatt agcttgtaca gtacttgcgg
     9841 gtgctgcggt tcttggtctt gctgcttgtg gcaattctgg cggaagtaaa gatgctgcca
     9901 aatcaggtgg tgacggtgcc aaaacagaaa tcacttggtg ggcattccca gtatttaccc
     9961 aagaaaaaac tggtgacggt gttggaactt atgaaaaatc aatcatcgaa gcgtttgaaa
    10021 aagcaaaccc agatataaaa gtgaaattgg aaaccatcga cttcaagtca ggtcctgaaa
    10081 aaatcacaac agccatcgaa gcaggaacag ctccagacgt actctttgat gcaccaggac
    10141 gtatcatcca atacggtaaa aacggtaaat tggctgagtt gaacgacctc ttcacagatg
    10201 aatttgttaa agatgtcaac aatgaaaaca tcgtacaagc aagtaaagct ggagacaagg
    10261 cttatatgta tccgattagt tctgccccat tctacatggc aatgaacaag aaaatgttag
    10321 aagatgctgg agtagcaaac cttgtaaaag aaggttggac aactgatgat tttgaaaaag
    10381 tattgaaagc acttaaagac aagggttaca caccaggttc attgttcagt tctggtcaag
    10441 ggggagacca aggaacacgt gcctttatct ctaaccttta tagcggttct gtaacagatg
    10501 aaaaagttag caaatataca actgatgatc ctaaattcgt caaaggtctt gaaaaagcaa
    10561 ctagctggat taaagacaat ttgatcaata atggttcaca atttgacggt ggggcagata
    10621 tccaaaactt tgccaacggt caaacatctt acacaatcct ttgggcacca gctcaaaatg
    10681 gtatccaagc taaactttta gaagcaagta aggtagaagt ggtagaagta ccattcccat
    10741 cagacgaagg taagccagct cttgaatatc ttgtaaacgg gtttgcagta ttcaacaata
    10801 aagacgacaa gaaagtcgct gcatctaaga aattcatcca gtttatcgca gatgacaagg
    10861 agtggggacc taaagacgta gttcgtacag gtgctttccc agtccgtact tcatttggaa
    10921 aactttatga agacaaacgc atggaaacaa tcagtggctg gactcaatac tactcaccat
    10981 actacaacac tattgatgga tttgctgaaa tgagaacact ttggttccca atgttgcaat
    11041 ctgtatcaaa tggtgacgaa aaaccagcgg atgctttgaa agccttcact gaaaaagcaa
    11101 acgaaacaat caaaaaagct atgaaacaat agtccttagt tattctatga aaaagtaatt
    11161 ttttaaagaa cctaagagtg tataccccct tttccctcta cacagatagt gtaagaaaag
    11221 ggggcttttg tttaaaatgt aagaaactgt cacgaaatta aaatgaagtt cttacataag
    11281 cgaaccataa aaaatttcat tttgatttta aaacagttca agaaagtcaa aaaattattc
    11341 tatttgaaag agaggtgccg actgtgaaag tcaataaaat ccgtatgcgg gaaacagtga
    11401 tttcctacgc tttcctagca ccagtattat tcttctttgt catctttgtg ttggctccga
    11461 tggtgatggg cttcattaca agtttcttta actactcaat gactaaattt gagtttgtag
    11521 gcttggataa ctatatccgt atgtttaaag atcctgtctt tacaaaatct ctgattaaca
    11581 cagttatttt ggttattgga tctgtaccag ttgttgttct attctcactc tttgtagcat
    11641 ctcagaccta tcatcaaaat gtcattgcca gatccttcta ccgtttcgtc ttcttccttc
    11701 ctgttgtaac gggtagtgtt gccgtgacag ttgtttggaa atggatttat gacccactat
    11761 cagggattct aaactttgtc cttaagtcaa gccacatcat cagccaaaac atttcttggt
    11821 tgggagataa aaactgggca ttgatggcga ttatgattat tctcttgacc acttcagttg
    11881 gtcagcccat catcctttat atcgctgcca tggggaatat tgacaattca ctggttgaag
    11941 cggcgcgtgt tgatggtgca actgagtttc aagttttttg gaagattaaa tggccaagcc
    12001 ttcttccaac aactctttat attgcaatca tcacaacaat taactcattc cagtgtttcg
    12061 ccttgattca gcttttgaca tctggtggtc caaactactc aacaagtacc ttgatgtact
    12121 acctttacga aaaagccttc caattgacag aatacggcta tgccaacaca attggtgtct
    12181 tcttggcagt catgattgct atcgtaagct ttgttcaatt taaagtactt ggaaacgacg
    12241 tagaatacta aagaaaggag acagctatgc aatctacaga aaaaaaacca ttaacagcct
    12301 ttactgttat ttcaacaatc attttgctct tgttgactgt gctgttcatc tttccattct
    12361 actggatttt gacaggggca ttcaaatcac aacctgatac aattgttatt cctcctcagt
    12421 ggttccctaa aatgccaacc atggaaaact tccaacaact catggtgcag aaccctgcct
    12481 tgcaatggat gtggaactca gtatttatct cattggtaac catgttctta gtttgtgcaa
    12541 cctcatctct agcaggttat gtattggcta aaaaacgttt ctatggtcaa cgcattctat
    12601 ttgctatctt tatcgctgct atggcgcttc caaaacaagt tgtccttgta ccattggtac
    12661 gtatcgtcaa cttcatggga atccacgata ctctctgggc agttatcttg cctttgattg
    12721 gatggccatt cggtgtcttc ctcatgaaac agttcagtga aaatatccct acagagttgc
    12781 ttgaatcagc taaaatcgac ggttgtggtg agattcgtac cttctggagt gtagccttcc
    12841 cgattgtgaa accagggttt gcagcccttg caatctttac cttcatcaat acttggaatg
    12901 actacttcat gcagttggta atgttgactt cacgtaacaa tttgaccatc tcacttgggg
    12961 ttgcgaccat gcaggctgaa atggcaacca actatggttt gattatggca ggagctgccc
    13021 ttgctgctgt tccaatcgtc acagtcttcc tagtcttcca aaaatccttc acacagggta
    13081 ttactatggg agcggtcaaa ggataatact ctgcgaaaat cgaatgcaaa ctacgtcagc
    13141 ttcaccttgc catacttaag tattgcctgt ggttagcttc ctagtttgtt cttcaatttt
    13201 cattgagtat aggaaaatca atctatcaaa atacagaagt atattttata gatttagaga
    13261 atatagaagt tataagtgtc tacaaaatgg agggtatgca gttactttat gaagttttgt
    13321 cagacactta tcaacttaag aatggtttta gttaactatc agaaacgaag gaaagagtat
    13381 gatttttgac gatttgaaaa acatcacctt ttacaaaggg attcatccca atttagacaa
    13441 ggctatcgac tatctctacc aacatcgtaa agattcattc gaattaggaa agtatgagat
    13501 tgatggagat aaagtctttc tagttgttca ggaaaatgtc ctcaatcaag ttgagaataa
    13561 tcaatttgaa caccataaga actatgcaga tttgcatttg ctggtagaag gacatgaata
    13621 ttcgagctac ggttcacgta tcaaagacga ggcagtagca ttcgacgaag cgagtgacat
    13681 tggctttgtt cattgtcatg aacactaccc actcttgttg ggttatcaca attttgcgat
    13741 tttcttccca ggtgagccac atcagccaaa tggttatgca ggcatggaag aaaaggttcg
    13801 aaaatatctc tttaaaattt tgattgatta aaaataggat gaattgtttt tttgtaaagc
    13861 tttgacaata ctctaccatg aaattgatct ttgtgaggta gggaaatgag aataaaatat
    13921 ttaaaaattg gtatcttctt gtcagtctgt gtatgctaca agagctagtt tcttggatgg
    13981 acagggaatt acagttgatg agatggcttg gataattagg ggcattgtga atgcattgat
    14041 tggtagatac ataaaattag gtacttatgc ggctaagtat ggtattagta tggcgcgttc
    14101 tatcttaaat agggcagctg caactgcggc agcaagagta ggattattga ctaagatttt
    14161 tggatggatt ttacgaatag ctgtgctcgc agctgatgta tatggtaatt ttgccaacaa
    14221 tattgctgca gcttgggatg cacatgataa aatccctaac aatggtcgta taaactttta
    14281 aaatgcgaga atgaaagcac tttgtatttt tttattgaat atgttagctt ggacagtgct
    14341 tgcaatgata gtccgtggag ggctagatgg atttaatgag catacttgga gtactatttt
    14401 aattgcttcg ctgtccggga tatatataga ttatatgtcc atagataaaa gtagaaaaaa
    14461 gtccaaaaga aaaaagaaaa aatagatttg ttcatggtag ggacttatga aagctttact
    14521 gacaaaaaag aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag
    14581 gagtaagcct tatcaaggca gcatttgata cagacaactt tctcatgcgt tttagtgaga
    14641 aagtcttgga catcgtgaca gccaatcttc tttttgtcgt ctcttgtttg cccatcgtga
    14701 cgattggagt ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac
    14761 gggtgcctgt ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc
    14821 agctgggttt aatggagtta ggaattgtgt ttcttaccct ttcagatctc tatcttttct
    14881 ggggtcaaac agctctgccc ttccaattgc tgaaagccat ttgtttaggt attctgattt
    14941 ttcttactat cgtgatgctg gctagttacc ctatcgcggc acgttatgac ctatcttgga
    15001 aagaaattct tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca
    15061 tgttagccat tcttgtcctc attgtgatgg ttctttatct gtccgccttc agtctactct
    15121 taggtggctc agtcttctta ctttttgggt ttggactatt ggtctttatc cagactggat
    15181 tgatggagaa aattttcgca aaataccaat aggagcctta tttctgaaac tactttcaaa
    15241 cgctccaaac gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa
    15301 agccttttcg gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata
    15361 aaaataatag gagaaaatct atgtcagatt tgaaaaaata cgaaggtgtc attccagcct
    15421 tctacgcatg ttatgatgat caaggagaag taagcccaga acgtacgcgt gccttggttc
    15481 aatacttcat tgataaaggt gttcaaggtc tttatgtcaa tggttcttct ggtgaatgta
    15541 tctaccaaag cgttgaagat cgcaagttga ttttggaaga agtcatggcg gtagccaaag
    15601 gtaaattgac catcattgct catgttgcct gcaacaatac taaagatagt atggaacttg
    15661 ctcgccatgc tgaaagcttg ggagtagatg ctattgcaac gattccacca atttatttcc
    15721 gcttgccaga atactcagtt gccaaatact ggaacgatat cagttctgca gctccaaaca
    15781 cagactacgt gatttacaac attcctcaat tggcaggggt tgctttgact ccaagccttt
    15841 acacagaaat gttgaaaaat cctcgtgtta tcggtgttaa gaactcttct atgccagttc
    15901 aagatatcca aacctttgtc agccttggtg gggaagacca tatcgtcttt aatggtcctg
    15961 atgagcagtt tctaggagga cgcctcatgg gtgctagggc tggtatcggt ggtacttatg
    16021 gtgctatgcc agaactcttc ttgaaactca atcagttgat tgcggataag gacctagaaa
    16081 cagcgcgtga attgcagtat gctatcaacg caatcattgg taaactcact tctgctcatg
    16141 gaaatatgta cggtgtcatt aaagaagtct tgaaaatcaa tgaaggcttg aatattggat
    16201 ctgttcgttc accattgaca ccagtgactg aagaagatcg tccagttgta gaagcggctg
    16261 ctgccttgat tcgtgaaacc aaggagcgct tcctctaatc taaaaggagg tatttatgac
    16321 atactacgtt gcaattgata tcggtggaac caacatcaag tatggtttgg ttgatcaaga
    16381 ggggcaactt cttgaatcgc atgaaatgcc aactgaggcg cataagggtg gacctcatat
    16441 cttacaaaag accaaagata tcgtagctag ttatttagaa aaaggcccag tagcaggtgt
    16501 tgccatatct tctgctggga tggtggatcc ggataagggt gagattttct atgctgggcc
    16561 tcaaatccct aactacgcag gcacccagtt caaaaaggaa atcgaagaaa gctttactat
    16621 tccttgtgag attgaaaatg atgtcaactg tgcaggtctt gctgaggcag tatctggttc
    16681 aggcaaggga gcaagtgtga cactttgctt gaccattgga accggtatcg gtggttgctt
    16741 gattatggat aggaaagtct tccatggttt tagcaattca gcctgtgaag tcgggtatat
    16801 gcatatgcag gatggagctt ttcaggatct cgcttctaca acagctttag tgaaatatgt
    16861 agctgaagcc catggagaag atgttgatca gtggaatggc cgacgtatct ttaaggaagc
    16921 tactgaagga aacaaaatct gcatggaagg tattgaccgt atggttgact atctaggaaa
    16981 aggtctggca aatatttgtt atgtggccaa tccagaagtg gttattcttg gtggtggtat
    17041 catggggcaa gaggctatcc tcaaacctaa gatccgcaca gccttgaaag aggcattggt
    17101 accaagttta gcagaaaaaa cacgattaga atttgcccat caccaaaata cagcagggat
    17161 gttgggtgca tattatcatt ttaagacaaa acaatcctag tttggctcag ccaaactagg
    17221 attttcttac acgtttttgt ctacgatagc cgttgagttt tttattttcc cagtagctat
    17281 taaagatttt ttccttgctt tcgcgattga tttccaaaaa gtaggcataa atcaaatcga
    17341 taaagaagag cataggaagt tgagcggata ttcgttggat gtaggagggt tggctgtggg
    17401 tggctacaag aacagtctct gtataggtct ggctatcttt attgggaaca cttgtaaaga
    17461 gtacagtctt tgcccccatc tccttggcat ctaatagact atctaaaata gaaggagttg
    17521 agcctgaaag tgagaaaccc agtactagac aattttcatc catgatgctg gttgtccagg
    17581 caaagccgtc ttggtctgtc aaagcttcgc agaccacacc tagtcgcata aaacgtaatt
    17641 tcatttcacg ggcgacgagg ccagaactcc ctgttccaaa gaagtagata cgctcagcat
    17701 cttcgattag ctgggcaatt cgctctagtt ggatttcgtc aatcaagtct tgtgtttgtt
    17761 ccctcatatt gctataactt ctgaggactc gtttggtcag tggactatgc ttggagactt
    17821 ggttggcttg attttctgcc tgatgttggt attggaaaat aaattctcgg tagccagtaa
    17881 agccacactt tttagcaaag cgggtcaaag cagcttgaga aatatgtaat ttttgggtga
    17941 cttgttgaga agataaatca tctgtaatcg tttcagcttg caaaaaatag cgagcgattt
    18001 cttgctctag gtctgtcatt tcttcaaaat gtgaatcaat gatagttgcg atatctggtt
    18061 tgtccatagg gaaagctcct ttacatgggt catactggaa gactagatca gggaatagtc
    18121 acacttcatt ataacacata atataaggat agataaataa aaacgcatct ctgttttaaa
    18181 aacgaaaaaa tcgaaaaagc ttctctcttt tccataattt tctgctcaaa ttgtggtaca
    18241 attaagagta agattttaag ttagaaatga gactgatttg tatgagaaaa tttaacagcc
    18301 attcgattcc gattcggctt aatttattgt tttcaatcgt cattttgctc tttatgacca
    18361 ttattggtcg tttgttgtat atgcaggttt tgaacaagga tttttacgaa aaaaagctag
    18421 cctcagctag tcagaccaag gtcacaacca gttctgctcg tggggaaatt tatgatgcta
    18481 gtggaaaacc tttggtagaa aatacgttaa agcaggttgt ttcctttacg cgtagtaata
    18541 aaatgacggc tacagactta aaagaaatag ctaaaaagtt actgacttat gtgagcatca
    18601 gttcgccaaa tttgacagaa cgccagctgg ctgattacta tttggctgat cctgaaatct
    18661 ataaaaaaac agtggaagct ctcccaagtg agaaacgctt ggattcagat ggcaatcgcc
    18721 tatccgaatc agaactgtat aacaatgcgg tcgatagtgt cccaacaagt caactaaact
    18781 atacagagga tgaaaagaaa gaaatctatc tttttagtca gttaaatgct gttggaaact
    18841 ttgcgacagg aaccattgcg acagatcctc taaatgattc tcaggtggct gttattgcct
    18901 ctatttcaaa ggagatgcct ggcattagta tttctacttc ttgggatcga aagattttgg
    18961 aaacttccct ttcttctata gtagggagtg tatccagtga aaaagccggt ctcccagcgg
    19021 aagaagcaga agcctatctt aaaaagggct attctctaaa tgaccgtgtt ggaacctcct
    19081 atttggaaaa gcaatatgaa gagaccttac aaggaaaacg ctcggtaaaa gaaatccatc
    19141 tggataaata tggcaatatg gaaagcgtgg acacaattga ggaaggtagt aagggaaaca
    19201 atatcaaact gaccattgat ttggccttcc aagatagcgt ggatgctttg ctgaaaagtt
    19261 atttcaattc cgagctagga aatggtggag ccaagtattc tgagggtgtg tatgcagtcg
    19321 cccttaaccc caaaacaggt gctgttttgt ctatgtcagg actcaaacat gacctgaaaa
    19381 cgggagagtt gactcctgat tccttgggaa cggtaaccaa tgtctttatc ccaggttcgg
    19441 ttgttaaggc cgctaccatc agctcaggtt gggaaaatgg tgttttatca ggaaaccaaa
    19501 ccttaacaga tcagcctatt gttttccaag gttcagctcc aatttattct tggtataaat
    19561 tggcatatgg atcttttcct attacagctg tggaagcctt ggagtattca tccaatgctt
    19621 acatggttca aaccgctctt ggaatcatgg gccagaccta tcaaccaaat atgtttgttg
    19681 gaaccagcaa tttggaaaca gctatgggaa aacttcgtgc gacctttggc gaatatggct
    19741 tgggggctgc gaccggaatt gacctaccat atgaatctac tggatttgtt cccaaagagt
    19801 atagctttgc taattacatc accaatgcct ttgggcagtt tgataactat acgcccatgc
    19861 agttggctca gtatgtagca actattgcaa atgatggtgt tcgtgtggct cctcgtattg
    19921 ttgaaggcat ttatggtaat aatgataagg gaggactggg tgacttgatt cagcaactgc
    19981 aaccgacaga gatgaataag gtcaatatat ccgactccga tatgagtatc ttgcaccaag
    20041 gattttacca agtatcgcat ggaactagtc cccttacgac aggacgggcg ttttcagatg
    20101 gcgccactgt ttctatcagt ggtaagaccg gtacaggtga aagctatgta gctggtggtc
    20161 aagaagctaa taataccaat gccgtggcct atgctccaac agaaaatcct caaattgcag
    20221 ttgcagtagt ctttcctcat aataccaatt taaccaaaaa tgttgggcca gcaattgctc
    20281 gcgacattat caatttatat aaccaacacc atccaatgaa ctagaaagga agccatgctt
    20341 tatccaacac ctattgctaa gttgattgac agttattcca aacttcctgg tattgggatt
    20401 aagacagcaa cccgtctagc cttttatact attggaatgc cggatgatga cgtcaacgaa
    20461 tttgctaaaa atctcctttc agctaaaaga gagttgacct attgttctat ctgtggacgt
    20521 ttaaccgatg aagatccctg ttctatctgc acagatccaa gtcgggacca aacaactatc
    20581 ctcgtcctag aggatagtcg agatgtagct gctatggaaa atatccaaga ataccacgga
    20641 ctctatcatg tcctgcatgg tctcatttct cctatgaatg gcattagtcc agatgatatc
    20701 aatctcaaga gtctcatgac ccgtcttatg gatagtgagg tttcagaagt aatcgtagca
    20761 accaatgcca cggcagatgg ggaagcgact tccatgtatc tttcgcgctt actcaagcca
    20821 gctgggatca aggttacgcg cctggcacga ggccttgctg tgggagcaga tatcgagtat
    20881 gcggacgaag tgaccctctt acgggctatt gaaaatcgaa cagagttgta agtgtagaca
    20941 aatttacgaa ctccattcat ttataaaaaa ttaaggaggc tgaaaatcgt tcctatcggc
    21001 ctctttttgt atagtgtgat gaataggatc aggttcaagt tttaaaaaaa caagcaaata
    21061 tgatatacta aagagcgagt attctagtag aattaggaca aataatatga aacaaacgat
    21121 tattctttta tatggtggac ggagtgcgga acgcgaagtc tctgtccttt cagcggaaag
    21181 tgtcatgagt gcggtcaatt acgaccgttt cacagtcaag actttcttta tcagccagtc
    21241 aggtgacttt atcaaaacgc aggaatttag tcagactcca ggtcaagagg accgtctcat
    21301 gaccaatgcg accattgatt gggataagaa agttgtacca agtgccatct acgaagaagg
    21361 tgcagttgtc tttccagttc ttcacggtcc gatgggagaa gatggctctg tccaaggatt
    21421 cttggaagtt ttgaaaatgc cgtatgtcgg ctgcaatatc ttgtcatcga gtcttgccat
    21481 ggataaaatc acgaccaagc gtgtgttaga atctgctggc attgcccaag ttccttatgt
    21541 ggctatcgtt gaaggggatg atgtgactgc taaaatcgct gaagtggaag aaaaattgac
    21601 ttatccagtc ttcactaagc cgtcaaacat ggggtctagt gtcggtattt ctaagtctga
    21661 aaatcaagaa gaactccgtc aggctttgga acttgccttc cgatatgaca gtcgtgtctt
    21721 ggtagagcaa ggggtaaatg cccgtgaaat cgaggttggt ctactgggca actacgatgt
    21781 caaaagcacc cttccaggag aagtagtcaa ggatgttgcc ttttatgact acgatgccaa
    21841 gtatattgac aacaagatta ccatggacat tccagctaaa atcagtgatg atgtggtagc
    21901 tgtcatgcgt cagaatgcag aaacagcctt ccgtgccatt ggtggcctcg gtctctctcg
    21961 ttgcgatttc ttctatacag acaagggaga gattttcctc aacgagctca ataccatgcc
    22021 aggattcacc aagtggtcaa tgtatccact tctttgggac aatatgggaa tcagttaccc
    22081 agacctaatc gagcgtttgg ttaatcttgc taaagaaagt tttgacaagc gcgaagcgca
    22141 tttgctataa aaatgaaaga gagggtagaa gccagaacca tcactgtaag gtgattagag
    22201 ttcttggact tcaacccttt ttaaaggagt agaaatgaaa ttaacaatct atgaagttgc
    22261 ccaagctgtt ggagctaaaa atgatgttag tctttttgcg gacgcccagt tagaaaaggc
    22321 tgagtttgac agtcgattaa ttgggactgg agatttattt gtgcccctca agggtgcgcg
    22381 tgatggccat gactttatcg aaacagcttt tgaaaatggc gctgctgtaa ccttgtctga
    22441 gaaagagctt gtaaatcatc cctatattct agtagatgat gtattgactg cctttcaaac
    22501 cttagctgcc tactatcttg aaaaaacagg agttgatgtc tttgcagtta caggttcaaa
    22561 tggcaagacg acgaccaagg atatgttggc gcatttacta tcaacaacct acaaaaccta
    22621 caaaacacaa ggaaactaca ataacgagat tggccttccc tacaccgttc tccatatgcc
    22681 tgaaggaaca gaaaagttgg tcttggagat ggggcaggat cacttgggag atattcatct
    22741 cttgtcagaa ttggctcatc caaagacagc tatcgttacc ttggtggggg aagctcattt
    22801 ggcctttttc aaagaccgtt cggaaatcgc taaagggaaa atgcaaattg ctgatggcat
    22861 gcctgaaggt tctttgctct tggccccagc agacccgatt gtagaggact acttgcctac
    22921 tgataaaaag gtggtccgtt ttgggcaagg agcagagctg gaaatcacag acttgattga
    22981 gcgcaaggat agtctgacct ttaaggctaa tttcttggaa caagcccttg atttgccagt
    23041 gactggtaag tacaatgcca ctaatgctat gattgcatcc tatgttgccc tgcaagaagg
    23101 agtgtcagag gagcaaattc atcaggcatt ccaaaatctt gaattgacgc gtaaccgtac
    23161 cgagtggaag aaagcagcca atggagcaga tattttgtct gatgtataca atgccaatcc
    23221 aactgctatg aagttgattt tagagacttt ttcggctatt ccagccaacg aaggtggcaa
    23281 aaaaatcgct gtcttggcgg atatgaagga actcggtgac cagtctgttc agctccataa
    23341 ccagatgatt ttgagcctct cgccagatgt gctggatgcc gtgattttct acggagaaga
    23401 cattgctgaa ttagcccaat tggctagtca aatgttccca ataggccacg tttactactt
    23461 caagaaaaca gaagaccaag accaatttga agacctagtc aagcaggtca aagaaagcct
    23521 tggagcctat gaccaaatct tgctaaaagg ttctaactct atgaatctag ccaagttggt
    23581 agaaagttta gaaaatgaag acaagtgatt ttgtcaagta tttgcaaaga atgattgcca
    23641 ttacagatac tggcttaacc tttacaaaag atccgtttga ccgtgagcgc tacgaagact
    23701 tgcgagacct tttatctgaa atgttgaatc agggatcaga ccttgattcc gaagaagtgg
    23761 cagaagtctt gaagccaact tctgcttatg cgactccgtt aatggacgtc cgtgcttgga
    23821 ttgttgagga cgaaaagatt tgtctggtta ggggacaagg agaggatagt tgggctttgc
    23881 caggtggctt tggtgaggtt ggctattctc caacagaaaa tattctcaag gaaattgaag
    23941 aagaaaccgg ttttaaagcc aaagttgaaa gactgctagc tgtttttgat accaatcgtt
    24001 tccaactaca gagcaaacag tatacaaagt ttgtttttgg atgtaagctt cttgatggac
    24061 aattccaaga aaatcaagaa attgctgacc ttcaattttt tgccattgac caactgccga
    24121 acttatctga aaaacgcatt accaaggagc aaatagagct tctttggcag gtttatcaag
    24181 gtcatagggg gcaatatctt gactaagaag atgattatcg tatttctaaa tccattttta
    24241 acaactagca tggtataata atatgcagga aaattttgaa ttatgaggaa gactagatga
    24301 atttatggga tattttcttt acgactcagg caaccgagcc gcccaaattt gacctttttt
    24361 ggtatgttag cctatttacg ctcttagcct taacttttta tacagcccat cgctatcgtg
    24421 aaaagaaggt ttaccaacga tttttccaaa tcttgcagac tgttcagtta atccttcttt
    24481 atggttggta ctgggttaat catatgccac tgtcagaaag cctacccttt taccattgcc
    24541 gtatggctat gtttgtggta ctcttgcttc ctggtcagtc caaatataaa caatactttg
    24601 cattattggg aacatttggg acattagcag cctttgttta tccagtgcca gatgcttacc
    24661 cttttccaca tattaccatt ttatccttta tctttggtca tttagcactc ttggggaact
    24721 ctctagttta tctattgaga cagtataatg cgcgattgct ggatgtgaag ggaatttttc
    24781 tcatgacctt tgccctaaat gccttgattt ttgtggtcaa tttggtgaca ggtggcgatt
    24841 acggattttt gacaaaaccg ccattggttg gggatcacgg tctagtagct aattatttac
    24901 ttgtttcaat tgtgctggta gctactatca gtttgactaa gaaaatctta gaattctttt
    24961 tagctcaaga agcagaaaaa atgattgcaa aggaagctta acacagagct ttcttttttg
    25021 ctcttagaga gtttttacaa gcagcttata aaataagaat ttctgaatag acaaactcaa
    25081 aaaatggctg ggaaatttag gaaaaaagca agcacgatta aattttttgt gttataatat
    25141 tttgtgaata gctatgccta tgtttagcta tggaataata cgaagtgcga aacttggaag
    25201 atagagagga agcgatgtaa tggctagaga aggctttttt acaggtctag atattggaac
    25261 aagctctgtc aaggtgcttg tggccgagca gagaaatggt gaattaaatg taattggcgt
    25321 gagtaatgcc aaaagtaaag gtgtaaagga tggaattatt gttgatattg atgcagcagc
    25381 aactgctatc aagtcagcca tttcccaagc ggaagaaaag gcaggcattt cgattaaatc
    25441 agtgaatgtc ggcttgcctg gtaatctttt gcaggtagaa ccaactcagg ggatgattcc
    25501 agtaacatct gatactaagg aaattacgga tcaagatgtt gaaaatgttg tcaaatcagc
    25561 tttgacaaag agtatgacac ctgaccgtga agtcattacc tttattcctg aagaatttat
    25621 tgtggatggt ttccaaggga ttcgtgaccc acgtggcatg atgggggttc gccttgaaat
    25681 gcgtggtttg ctttatacag gacctcgtac tatcttgcac aatttgcgta agacggttga
    25741 gcgtgcaggt gttcaggttg aaaatgttat catttcacca ctagcaatgg ttcagtctgt
    25801 tttgaacgaa ggggaacgtg aatttggtgc tacagtgatt gatatggggg caggtcaaac
    25861 gactgtcgct acaatccgta atcaagaact ccagttcaca catattctcc aagaaggtgg
    25921 agattatgta actaaagata tctccaaggt tttgaaaacc tctcgcaaat tagcggaagg
    25981 cttgaaactg aattacgggg aagcctatcc gcctcttgca agcaaagaaa ccttccaagt
    26041 agaagttatt ggagaagtag aagcagtcga agtgacggaa gcctacttgt cagaaattat
    26101 ttctgcacga atcaagcaca tccttgaaca aatcaagcaa gaattagata gaaggcgtct
    26161 attggacctc cctggtggta ttgtcttaat cggtgggaat gccattttac caggtatggt
    26221 tgagcttgct caggaagtct ttggcgtccg tgtcaagctt tatgttccaa atcaagttgg
    26281 tatccgtaat ccagcctttg cgcatgtgat tagtttatca gaatttgcgg gtcaattaac
    26341 agaagttaat cttttggctc agggagcgat aaaaggtgag aatgacttaa gtcatcagcc
    26401 aattagtttt ggtgggatgc tgcaaaaaac agctcagttt gtacaatcaa cgcctgttca
    26461 accagctcct gctccagaag tagagccggt ggcgcctaca gaaccaatgg cggatttcca
    26521 acaagcttca caaaataaac cgaaattagc agatcgtttc cgtggcttga tcggaagcat
    26581 gtttgacgaa taaagaggaa aaataaatta tgacattttc atttgataca gctgctgctc
    26641 aaggggcagt gattaaagta attggtgtcg gtggaggtgg tggcaatgcc atcaaccgta
    26701 tggtcgacga aggtgttaca ggcgtagaat ttatcgcagc aaacacagat gtacaagcat
    26761 tgagtagtac aaaagctgag actgttattc agttgggacc taaattgact cgtggtttgg
    26821 gtgcaggagg tcaacctgag gttggtcgta aagccgctga agaaagcgaa gaaacactga
    26881 cggaagctat tagtggtgcc gatatggtct tcatcactgc tggtatggga ggaggctctg
    26941 gaactggagc tgctcctgtt attgctcgta tcgccaaaga tttaggtgcg cttacagttg
    27001 gtgttgtaac acgtcccttt ggttttgaag gaagtaagcg tggacaattt gctgtagaag
    27061 gaatcaatca acttcgtgag catgtagaca ctctattgat tatctcaaac aacaatttgc
    27121 ttgaaattgt tgataagaaa acaccgcttt tggaggctct tagcgaagcg gataacgttc
    27181 ttcgtcaagg tgttcaaggg attaccgatt tgattaccaa tccaggattg attaaccttg
    27241 actttgccga tgtgaaaacg gtaatggcaa acaaagggaa tgctcttatg ggtattggta
    27301 tcggtagtgg agaagaacgt gtggtagaag cggcacgtaa ggcaatctat tcaccacttc
    27361 ttgaaacaac tattgacggt gctgaggatg ttatcgtcaa cgttactggt ggtcttgact
    27421 taaccttgat tgaggcagaa gaggcttcac aaattgtgaa ccaggcagca ggtcaaggag
    27481 tgaacatctg gctcggtact tcaattgatg aaagtatgcg tgatgaaatt cgtgtaacag
    27541 ttgtcgcaac gggtgttcgt caagaccgcg tagaaaaggt tgtggctcca caagctagaa
    27601 ctgctactaa ctaccgtgag acagtgaaac cagctcattc acatggcttt gatcgtcatt
    27661 ttgatatggc agaaacagtt gaattgccaa aacaaaatcc acgtcgtttg gaaccaactc
    27721 aggcatctgc ttttggtgat tgggatcttc gccgtgaatc gattgttcgt acaacagatt
    27781 cagtcgtttc tccagtcgag cgctttgaag ccccaatttc acaagatgaa gatgaattgg
    27841 atacacctcc atttttcaaa aatcgttaag taaatgaatg taaaagaaaa tacagaactt
    27901 gtttttcgag aagttgcaga ggctagtctg agtgctcatc gagagagtgg ttcggtctct
    27961 gtcattgcag ttaccaagta tgtagatgta ccgacagcgg aagccttgct tccgctaggt
    28021 gttcatcata tcggtgaaaa tcgtgtagat aagtttctgg aaaaatatga agctttaaaa
    28081 gatcgagatg tgacttggca tttgattggt accttgcaaa gacgtaaggt gaaagatatc
    28141 attcaatacg ttgattattt ccatgcattg gactcagtaa agctagcagg ggaaattcaa
    28201 aaaagaagtg accgagtcat caagtgtttc cttcaagtaa atatttctaa agaagaaagc
    28261 aaacacggtt tttcgagaga ggaactgctg gaaatcttgc cagagttagc cagactagat
    28321 aagattgaat atgttggttt aatgacgatg gcaccttttg aggctagcag tgagcagttg
    28381 aaagagattt tcaaggcggc ccaagattta caaagagaaa ttcaagagaa acaaattcca
    28441 aatatgccta tgaccgagtt aagtatggga atgagtcgtg attataaaga agcgattcaa
    28501 ttcggttcca cttttgttcg tataggtaca tcatttttta agtaggagag aaccatgtct
    28561 ttaaaagata gattcgatag atttatagat tattttacgg aggatgagga ttcaagtctc
    28621 ccttatgaaa aaagagatga gcctgtgttt acttcagtaa attcttcaca ggaaccggct
    28681 ctcccaatga atcaaccttc acagtcggct ggtacaaaag agaacaatat caccagactt
    28741 catgcaagac aacaggaatt ggcaaatcag agtcagcgtg caacggataa ggtcattata
    28801 gatgttcgtt atcctagaaa atatgaggat gcaacagaaa ttgttgattt attggcagga
    28861 aacgaaagta tcttgattga ttttcagtat atgacagagg tgcaggctcg tcgttgtttg
    28921 gactatttgg atggagcttg tcatgtttta gctggaaatt tgaaaaaggt agcttctacc
    28981 atgtatttgt tgacaccagt gaacgttatt gtaaatgttg aagatatccg tttaccagat
    29041 gaagatcaac agggtgagtt cggttttgat atgaagcgaa atagagtacg ataatgattt
    29101 ttttaattcg tatgatttat aatgcagtgg atatttactc cctgattttg gtagccttcg
    29161 ctgtcatgtc ttggtttcca ggtgcctacg aatccagttt aggtcgttgg attgtagcgt
    29221 tggtgaaacc agtgcttgct cccttgcaac gcctgccttt acagatagcg ggtcttgatt
    29281 tatctgtttg ggtcgcgatt gttttggttc gatttttagg agaaaaccta gtgcgttttc
    29341 tggcgatgat aggatgaata aagggattta tcagcatttc tccatagaag atcgtccatt
    29401 tcttgacaag ggaatggaat ggataaagaa ggtagaagat agctatgctc cttttttaac
    29461 tccttttatc aatcctcatc aggagaagct attaaagatt ttggccaaaa cctatggtat
    29521 tgcttgtagc agtagtgggg aattcgtctc gagtgagtat gttcgagttt tattataccc
    29581 agattatttc caaccagagt tttcagattt tgaaatatct ctccaggaaa ttgtgtattc
    29641 caataaattt gaacatttaa cgcatgctaa gattttaggg acagtcatca atcaattagg
    29701 gattgaacgg aaactttttg gagatatcct agtagatgaa gaacgggcgc agattatgat
    29761 taatcagcag tttcttcttc tctttcaaga tggactaaag aaaattggtc gtatacctgt
    29821 ttcgctggag gaacgtcctt tcaccgagaa aatagataag ctagaacagt atcgagaact
    29881 gaatttatct gtgtctagtt ttcgattaga tgttctttta tcaaatgttt tgaaactatc
    29941 taggaatcaa gcaaaccagt tgattgaaaa gaaacttgtc caagtaaatt atcatgtggt
    30001 agacaaatca gattacactg ttcaagttgg agacttgatt agtgtgagaa aatttggtcg
    30061 cttgagatta tttcaagata agggacaaac gaaaaaagag aagaaaaaaa taaccgtcca
    30121 gttattatta agtaagtgag gaatagaatg ccaattacat cattagaaat aaaggacaag
    30181 acctttggaa ctcgattcag aggttttgat ccagaagaag tcgatgaatt tttagatatt
    30241 gtggttcgtg attacgaaga tcttgtgcgt gcgaatcatg ataaaaattt gcgtattaag
    30301 agtttagaag agcgtttgtc ttactttgat gaaataaaag attcattgag ccagtctgta
    30361 ttgattgccc aggatacagc tgagagagtg aaacaggcgg cgcatgaacg ttcaaacaat
    30421 atcattcatc aagcagagca agatgcgcaa cgcttgttgg aagaagctaa atataaggca
    30481 aacgagattc ttcgtcaagc aactgataat gctaagaaag tcgctgttga aacagaagaa
    30541 ttgaagaaca agagccgtgt cttccaccaa cgtctcaaat ctacaattga gagtcagttg
    30601 gctattgttg aatcttcaga ttgggaagat attctccgtc caacagctac ttatcttcaa
    30661 actagtgatg aagcctttaa agaagtggtt agcgaagtac ttggagaacc gattccagct
    30721 ccaattgaag aagaaccaat tgatatgaca cgtcagttct cccaagcaga aatggaagaa
    30781 ttacaagctc gtattgaggt agccgataaa gaattgtctg aatttgaagc tcagattaaa
    30841 caggaagtgg aaactccaac tcctgtagtg agtcctcaag ttgaagaaga gcctctgctc
    30901 atccagttgg cccaatgtat gaagaaccag aagtagctcc agtgcatccg acaggtccaa
    30961 caccagctac agaaactgtt gattcagcac cgggatttga agcaccgcaa gaatctgtta
    31021 caattttata agaaatattc tgagaacaat atcttatcct tatattccca gcgagcagga
    31081 gatggtgtga gtcctgcatt ccctatcgat aagattatcc tctcaaaaac tcaagtctga
    31141 atctagtaag atttgacgtt ccccacgtta cgggataaga gggagaaaga ctaaaacttt
    31201 ttccgaataa aggtggtacc acgattttcg tcctttttgg aagtcgtggt ttttaatttg
    31261 ttattattta taaaggagat accatgaaac tcaaagacac ccttaatctt gggaaaactg
    31321 aattcccaat gcgtgcaggc cttcctacca aagagccagt ttggcaaaag gaatgggaag
    31381 atgcaaaact ttatcaacgt cgtcaagaat tgaaccaagg aaaacctcat ttcaccttgc
    31441 atgatggccc tccatacgct aacggaaata tccacgttgg acatgctatg aacaagattt
    31501 caaaagatat cattgttcgt tctaagtcta tgtcaggatt ttacgcacca tttattcctg
    31561 gttgggatac tcatggtctg ccaatcgagc aagtattgtc aaaacaaggt gtcaaacgta
    31621 aagaaatgga cttggttgag tacttgaaac tttgccgtga gtacgctctt tctcaagtag
    31681 ataaacaacg tgaagatttt aaacgtttgg gtgtttctgg tgactgggaa aatccatatg
    31741 tgaccttgac tcctgactat gaagcagctc aaattcgtgt atttggtgag atggctaata
    31801 agggttatat ctaccgtggt gccaagccag tttactggtc atggtcatct gagtcagccc
    31861 ttgctgaagc agagattgaa taccatgact tggtttcaac ttccctttac tatgccaaca
    31921 aggtaaaaga tggcaaagga gttctagata cagatactta tatcgttgtc tggacaacga
    31981 ctccatttac catcacagct tctcgtggtt tgacggttgg tgcagatatt gattacgttt
    32041 tggttcaacc tgctggtgaa gctcgtaagt ttgtcgttgc tgctgaatta ttgactagct
    32101 tgtctgagaa atttggctgg gctgatgttc aagttttgga aacttaccgt ggccaagaac
    32161 tcaaccacat cgtaacagaa cacccatggg atacagctgt agaagagttg gtaattcttg
    32221 gtgaccacgt tacgactgac tctggtacag gtattgtcca tacagcccct ggttttggtg
    32281 aggacgatta caatgttggt attgctaata atcttgaagt cgcagtgact gttgatgaac
    32341 gtggtatcat gatgaagaat gctggtcctg aatttgaagg tcaattctat gaaaaggtag
    32401 ttccaactgt tattgaaaaa cttggtaacc tccttcttgc ccaagaagaa atctctcact
    32461 catatccatt tgactggcgt actaagaaac caatcatctg gcgtgcagtt ccacaatggt
    32521 ttgcctcagt ttctaaattc cgtcaagaaa tcttggacga aattgaaaaa gtgaaattcc
    32581 actcagaatg gggtaaagtc cgtctttaca atatgatccg tgaccgtggt gactgggtta
    32641 tctctcgtca acgtgcttgg ggtgttccac ttcctatctt ctacgctgaa gatggtacag
    32701 ctatcatggt agctgaaact attgaacacg tagctcaact ttttgaagaa catggttcaa
    32761 gcatttggtg ggaacgtgat gccaaagacc tcttgccaga aggatttact catccaggtt
    32821 caccaaacgg cgagttcaaa aaagaaactg atatcatgga cgtttggttt gactcaggtt
    32881 catcatggaa tggagtggtg gtaaaccgtc ctgaattgac ttacccagcc gacctttacc
    32941 tagaaggttc tgaccaatac cgtggttggt ttaactcatc acttatcaca tctgttgcca
    33001 accatggcgt agcaccttac aaacaaatct tgtcacaagg ttttgccctt gatggtaaag
    33061 gtgagaagat gtctaaatct cttggaaata ctattgctcc aagcgatgtt gaaaaacaat
    33121 tcggtgctga aatcttgcgt ctctgggtaa caagtgttga ctcaagcaat gacgtgcgta
    33181 tctctatgga tatcttgagc caagtttctg aaacttaccg taagattcgt aacactcttc
    33241 gtttcttgat tgccaataca tctgacttta acccagctca agatacagtc gcttacgatg
    33301 agcttcgttc agttgataag tacatgacga ttcgctttaa ccagcttgtc aagaccattc
    33361 gtgatgccta tgcagacttt gaattcttga cgatctacaa ggccttggtg aactttatca
    33421 acgttgactt gtcagccttc taccttgatt ttgccaaaga tgttgtttac attgaaggtg
    33481 ccaaatcact ggaacgccgt caaatgcaga ctgtcttcta tgacattctt gtcaaaatca
    33541 ccaaactctt gacaccaatc cttcctcaca ctgcggaaga aatttggtca tatcttgagt
    33601 ttgaagccga agacttcgtc caattgtcag aattaccaga ggctcaaact tctgctaacc
    33661 aagaagaaat cttggataca tgggcagcct tcatggactt ccgtggacaa gcacaaaaag
    33721 ccttggaaga agctcgtaat gcaaaagtta tcgggaaatc acttgaagca cacttgacag
    33781 tttatccaaa tgaagtggtg aaaactctac tcgaagcagt aaacagcaat gtagctcaac
    33841 ttttgatcgt gtctgagttg accatcgcag aagaaccaac tccggaagct gcccttagct
    33901 tcgaagatgt agccttcaca gttgaacgtg ctgctggtga agtatgtgac cgttgccgtc
    33961 gtatcgaccc aacaacagca gaacgcagct accaggcagt tatctgtgac cactgtgcaa
    34021 gcatcgtaga agaaaatttt gcggatgcag tcgcagaagg atttgaagag aaataagatt
    34081 gaaaagtcta ggcaaaattc aatttgagaa gaaaagacaa ctaattttat agtctattaa
    34141 acgcattgta tcacgttttt gaacacctga tatgatgcgt ttttatttat tttgaaaatt
    34201 tgtgaggtgt gactttttat actcaacaag aatcaaagag aaacttagca agctaacagt
    34261 a
//
LOCUS       DAVHIU010000008        33501 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVHIU010000008 DAVHIU010000000
VERSION     DAVHIU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.008"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..1035
                     /locus_tag="VVN06_000314"
                     /pseudo
     CDS             1..1035
                     /locus_tag="VVN06_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1206..2768)
                     /gene="guaA"
                     /locus_tag="VVN06_000315"
     CDS             complement(1206..2768)
                     /gene="guaA"
                     /locus_tag="VVN06_000315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5561925.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2910..3608
                     /locus_tag="VVN06_000316"
     CDS             2910..3608
                     /locus_tag="VVN06_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5561926.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPERFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3651..4547)
                     /locus_tag="VVN06_000317"
     CDS             complement(3651..4547)
                     /locus_tag="VVN06_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5561927.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFTIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4556..5491)
                     /locus_tag="VVN06_000318"
     CDS             complement(4556..5491)
                     /locus_tag="VVN06_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU5561928.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5488..6213)
                     /locus_tag="VVN06_000319"
     CDS             complement(5488..6213)
                     /locus_tag="VVN06_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU5561929.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6297..6932)
                     /locus_tag="VVN06_000320"
     CDS             complement(6297..6932)
                     /locus_tag="VVN06_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU5561930.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     ETLNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(6934..7761)
                     /locus_tag="VVN06_000321"
     CDS             complement(6934..7761)
                     /locus_tag="VVN06_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5561931.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7783..8118)
                     /locus_tag="VVN06_000322"
     CDS             complement(7783..8118)
                     /locus_tag="VVN06_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU5561932.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(8119..8835)
                     /locus_tag="VVN06_000323"
     CDS             complement(8119..8835)
                     /locus_tag="VVN06_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5561933.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKAALLAYSYGFNGIEQYISKEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMLQMGVEYQLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            9348..9959
                     /locus_tag="VVN06_000324"
     CDS             9348..9959
                     /locus_tag="VVN06_000324"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5561934.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10004..10732
                     /gene="rlmB"
                     /locus_tag="VVN06_000325"
     CDS             10004..10732
                     /gene="rlmB"
                     /locus_tag="VVN06_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5561935.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10770..11681)
                     /gene="trxB"
                     /locus_tag="VVN06_000326"
     CDS             complement(10770..11681)
                     /gene="trxB"
                     /locus_tag="VVN06_000326"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5561936.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11747..11971)
                     /locus_tag="VVN06_000327"
     CDS             complement(11747..11971)
                     /locus_tag="VVN06_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU5561937.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12049..12792)
                     /locus_tag="VVN06_000328"
     CDS             complement(12049..12792)
                     /locus_tag="VVN06_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5561938.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRRLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12792..13592)
                     /locus_tag="VVN06_000329"
     CDS             complement(12792..13592)
                     /locus_tag="VVN06_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5561939.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13750..14106)
                     /locus_tag="VVN06_000330"
     CDS             complement(13750..14106)
                     /locus_tag="VVN06_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU5561940.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14100..14630)
                     /locus_tag="VVN06_000331"
     CDS             complement(14100..14630)
                     /locus_tag="VVN06_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5561941.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(14630..15124)
                     /locus_tag="VVN06_000332"
     CDS             complement(14630..15124)
                     /locus_tag="VVN06_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5561942.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15259..15705
                     /locus_tag="VVN06_000333"
     CDS             15259..15705
                     /locus_tag="VVN06_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU5561943.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15702..16349
                     /locus_tag="VVN06_000334"
     CDS             15702..16349
                     /locus_tag="VVN06_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU5561944.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(16370..16951)
                     /gene="pdxT"
                     /locus_tag="VVN06_000335"
     CDS             complement(16370..16951)
                     /gene="pdxT"
                     /locus_tag="VVN06_000335"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5561945.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16952..17827)
                     /gene="pdxS"
                     /locus_tag="VVN06_000336"
     CDS             complement(16952..17827)
                     /gene="pdxS"
                     /locus_tag="VVN06_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5561946.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17979..19358)
                     /locus_tag="VVN06_000337"
     CDS             complement(17979..19358)
                     /locus_tag="VVN06_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5561947.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19652..20575
                     /locus_tag="VVN06_000338"
     CDS             19652..20575
                     /locus_tag="VVN06_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5561948.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20635..21240
                     /locus_tag="VVN06_000339"
     CDS             20635..21240
                     /locus_tag="VVN06_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5561949.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
     gene            21258..22502
                     /locus_tag="VVN06_000340"
     CDS             21258..22502
                     /locus_tag="VVN06_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5561950.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGSHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(22619..22876)
                     /locus_tag="VVN06_000341"
     CDS             complement(22619..22876)
                     /locus_tag="VVN06_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU5561951.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22918..24954)
                     /locus_tag="VVN06_000342"
     CDS             complement(22918..24954)
                     /locus_tag="VVN06_000342"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU5561952.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVHIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(25214..26131)
                     /gene="glyQ"
                     /locus_tag="VVN06_000343"
     CDS             complement(25214..26131)
                     /gene="glyQ"
                     /locus_tag="VVN06_000343"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU5561953.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(26324..27085)
                     /locus_tag="VVN06_000344"
     CDS             complement(26324..27085)
                     /locus_tag="VVN06_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5561954.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(27358..28200)
                     /locus_tag="VVN06_000345"
     CDS             complement(27358..28200)
                     /locus_tag="VVN06_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5561955.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEKSVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(28314..29705)
                     /locus_tag="VVN06_000346"
     CDS             complement(28314..29705)
                     /locus_tag="VVN06_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5561956.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29940..30317)
                     /locus_tag="VVN06_000347"
     CDS             complement(29940..30317)
                     /locus_tag="VVN06_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU5561957.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            30532..31509
                     /locus_tag="VVN06_000348"
     CDS             30532..31509
                     /locus_tag="VVN06_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5561958.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            31506..32588
                     /locus_tag="VVN06_000349"
     CDS             31506..32588
                     /locus_tag="VVN06_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5561959.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     TEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(32652..33501)
                     /locus_tag="VVN06_000350"
                     /pseudo
     CDS             complement(32652..33501)
                     /locus_tag="VVN06_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9913 a         7522 c         6028 g        10038 t
ORIGIN      
        1 aaaatcacca aatccctcgt atcatcaacc aaaaaattgc gcaaaagttg attgagaaga
       61 tttctatgac cgatattgct catcagctgg ccatttcaac ttcaactgtc attcgcaagc
      121 tcaatgattc tcactttgag catgattttt cgcgtcttcc tgagattatg tcctgggacg
      181 ttgaaacagt ccggggagtg actgtttcaa tcgggagatg gagatgagct ttattgcgca
      241 agattttgaa aagctcgata tcatcactgt tcttgaaggt agaacacaag ctgtcatccg
      301 agatcacttt cttaaatatg atagagccgt ccgatgtcgc gtcaaaatca ttactatgga
      361 tatgtttagc ccttactatg acttggctaa acagcttcgc tttcaaattt ctaggctcag
      421 gctgaaacag tctcccagac tgtttcactc ccgaatgcta aaatcgttct tgatcgcttt
      481 cacattgtgc aacatcttag ccgtgctatg agtcgtgtgc gtgttcaaat catgaatcag
      541 tttcatcgaa aatcccatga atacaaggct atcaagcgct actggaagct catacaacag
      601 gatagccgta aactgagtga taagcgattt tatcgtccta cttttcgtat gcatttaacc
      661 aataaagaga ttttaaacaa gcttttgagt tattcacaag acttgaaaca ccactatcag
      721 ctctatcagc tcttgctttt tcacttccag aataaggaac cagagaaatt tttcgaactt
      781 atcgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa aaccttcctc
      841 aaagataaag aaaagattat caacgccctt caactacact attctaatgc caaactggaa
      901 gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg aaactttgaa
      961 aacttcaaaa aacggatttt tatcgctttg aacatcaaaa aagaaaggac gaaatttgtc
     1021 ctttctcgag cttagctgac ttcaacccac tacagttgac aaagagccat tttttgtcca
     1081 aatgtaaacc acttggaact attattcaat ttattctttt tagtaaatgt tcccgtttta
     1141 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
     1201 tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
     1261 ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga
     1321 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
     1381 tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg
     1441 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
     1501 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
     1561 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
     1621 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
     1681 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
     1741 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
     1801 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt
     1861 tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
     1921 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
     1981 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     2041 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     2101 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     2161 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     2221 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     2281 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     2341 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     2401 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     2461 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     2521 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     2581 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     2641 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     2701 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     2761 ttgctcatct ttttcctttc tcaaaagaaa tatcttttcc caatattcta tcacaaataa
     2821 gggcgaatta ccaactaaac aaggcaaagt ttgacttttt ctagtttatt ggggtacaat
     2881 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa atccatgatc
     2941 agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt ccaagtgaaa
     3001 gagatttagc tgagcagttt gcggtcagtc gtatgaccct ccgccaagcc gtatctctat
     3061 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     3121 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     3181 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     3241 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     3301 accaagttcc cctagtttat gaagttgctt ctattcctga aagattcatt aaggacttta
     3361 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggt tatcgtatcg
     3421 gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     3481 aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta
     3541 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ttttgaattt tatcttgaaa
     3601 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     3661 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     3721 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaac cgccgatatt
     3781 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     3841 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     3901 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct
     3961 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     4021 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     4081 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     4141 gtgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     4201 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     4261 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     4321 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     4381 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt ggttaagacc
     4441 agagccgaaa ttccgtgtgc catcaggtat tggccactcg ttcctgacaa cccccaagca
     4501 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     4561 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     4621 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     4681 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     4741 aagaacgttc cttgtcatcc aaaggagaaa aattttgact taagtctctt gcaaaatcat
     4801 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcgc
     4861 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     4921 ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc attccccaag
     4981 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     5041 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     5101 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     5161 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtaatgtct ggataatcac
     5221 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     5281 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     5341 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     5401 gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg atttgttctt
     5461 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     5521 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     5581 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     5641 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     5701 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     5761 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     5821 tcctacttct actagacttg ctctgtcatc ttccgcatga atcaaaatca aatcatcttc
     5881 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     5941 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     6001 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     6061 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     6121 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     6181 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     6241 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     6301 taaagatagt ctttcaaggc ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     6361 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     6421 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccaattc
     6481 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctcc ctcattgaca
     6541 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     6601 atagcttcga gattattgag agtctcattc acaggaacat cctttccgat atcatttgtc
     6661 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     6721 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcatctt tgaagttcca
     6781 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     6841 tctacaacag aaatgtgatt taggtgacga tatttggttt gaattttttc ttgttccttt
     6901 agaagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     6961 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     7021 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     7081 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     7141 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     7201 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     7261 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     7321 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     7381 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     7441 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gccaaagcct tgttgacaat
     7501 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     7561 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     7621 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     7681 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     7741 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     7801 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     7861 ctggtcacga tgactgccac cctctataaa atcttccaaa atataagttt ggtaatgata
     7921 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     7981 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     8041 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa tccgttgatt
     8101 ggaaatgaag ttccccattt aatagataat gagcttctta tctccatctt tttcaaccgt
     8161 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc cccaatcgat
     8221 catcttgtga taaagagctt tttgttcttc agttggttcc aattgatact caacacccat
     8281 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt cagccttcat
     8341 cttatcttcg tttaaatctg aaagataacg attatagtct tccttagaaa tatactgctc
     8401 aattccattg aaaccatagg aataggctaa caatgcagcc ttgataccat tcacttcctt
     8461 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     8521 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     8581 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     8641 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     8701 atcaccaata gctaaatctg cttgcttgag ccaaggagtc acatactcaa aattttcatg
     8761 aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt ggtagagcaa
     8821 atccccatgg gccataattc tggcagtttt ttcctcttcc tactcctgag acttttgctt
     8881 agtcccctct tgagaaatag tatctcgttt ttgactagtc aagggattcc ttggaagctt
     8941 tcaaacaaca agatcaagcc cattgataaa ccaactgcta gcaagagtat cgccacaaat
     9001 cccttattgc tccacttgcg ataactccta aaaagtttac caagcccttc ataaaacgaa
     9061 aagctaaacc accctgattt cgattttgtc ttctttgtat cttcgttctc cctactttct
     9121 tatgcaagcc ttttcttttt attatatcac agataagtat ttctttcaca attgaattga
     9181 acttcccatc tattttctat aaatcctaaa tgccataatg ctttcaattc ttgtcatttt
     9241 gtgatatcat gtagaggaaa tgaactaatc cacagtggct tattccaagt ataccacttg
     9301 ggctttggca gtaactaact gcactaaaca taatataagg agagaaaatg tctgcaatag
     9361 aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt gaagggaatc
     9421 ctactctacg cgcgattgct gaggaagtca ctttccccct atctgaccag gaaatcatcc
     9481 taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg gctgaaaaaa
     9541 tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca aaacgcatta
     9601 tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa gcctacgatt
     9661 tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat gctgctcttg
     9721 gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt gttcgccatg
     9781 cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc aaactcaaag
     9841 gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc atgttttacg
     9901 atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt cttgaataaa
     9961 gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa caaatgatat
    10021 tgtctatggc gtccacgccg ttaccgaagc cctccttgca aatacaggaa acaaactcta
    10081 cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag ctacagaaaa
    10141 gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagattactg aaggtgctgt
    10201 tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag attacatcct
    10261 tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc taaccgatcc
    10321 ccataatcta ggttctatct tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat
    10381 tcccaagcac cgtgctgtcg gagtaactcc tgtcgttgcc aaaacagcca caggtgctat
    10441 tgaacacgtt ccaattgccc gagtgaccaa cctcagtcaa accttagata aacttaagga
    10501 tgaaggtttc tggacctttg gaacggatat gaacggtact ccttgccaca agtggaatac
    10561 aaaagggaaa atcgccctca tcattggaaa tgaaggaaaa ggtatctcta gcaacatcaa
    10621 aaaacaagtc gatgagatga ttaccattcc gatgaatgga catgttcaaa gccttaatgc
    10681 cagtgttgct gcggccattc tcatgtacga agttttccga aatagactat aaaaagtttc
    10741 cagtcatctg attggaaact tttttataat taactatgtt ctgtaatgaa tttataggct
    10801 tcttgaccag cgatagctcc atctccaacc gctgttgtta cttggcgaag gtctttcaag
    10861 cgaacatctc caactgcaaa gataccgtcg actgcagttt tcatgtggtt atctgtcaca
    10921 atccatcctg cctgatcttg gatattcaat tctttaacaa aatcgctaag agggtccaaa
    10981 ccaacataga taaagacacc accgaagact tgttctgtca cttgacctgt tttcacattt
    11041 tcaaatacga ctgattctac tcggttttca cccttgattt cccttactac agaatcccag
    11101 ataaagctga ttttttcatt cgcaaaggcg cgatcttgta aaaccttttg ggcacgaagt
    11161 tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac gagtcaagaa gagggcttct
    11221 tcaacagctg aatctccacc accaactacc aataaatctt ggtcacggaa gaaagcacca
    11281 tcacacacag cacagtaaga aacaccacga ctgttcagtt cttcttctcc aggtactccc
    11341 aaaggacggt gtttagaacc agttgctacg ataactgtac gtgtttcata tgtttggtca
    11401 tcagtcatca ctttcttaaa atcaccatgg tcttcgacat tttcaacata accataaatg
    11461 tgctccacac caagattttc aagtggttca aacatctttt cagccaattc aggtccacta
    11521 atattagcgt atcctgggta attttcgata tcagatgtat tattcatctg accacctggc
    11581 agaccacctt caatcaaagc tacttttaga ttgcttcgag cagcatacaa ggccgcagtc
    11641 atccctgcag gtccagcacc gataataata gtatcgtaca tatagattcc ttctttcttg
    11701 ttgtaactat ctttattcta actctttctt gtcaatcacg caaggattat gccttgaaaa
    11761 caagaattaa actcataacc gcaaaggata atactgggac aaccaagact ccaatcataa
    11821 tcatatcata gagatgggct tgacgagccc tggctgtctt atcaactccc gacatggctc
    11881 tcagaccaat ccaaatccct aaaaaaatca gaacaaggat ggtggtcaag atcaaactct
    11941 cgaaatataa agaaaatagt tgcagtagca tgatttctct catttctatc ttttttaaag
    12001 agtaaactca gctagtccaa ctaactgagt tttcctttat ctattatatc aaatataagt
    12061 ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct ttaggggtgt ggataatctc
    12121 atccggagtt ccagactcga tgattttccc cttatctaag aagagaattt tatccgcaac
    12181 ttgggctaca aaggacatgt catgactgac caaaatcatg gtctgacctg acttagcagc
    12241 atctgcaata gacttttcta cttcaccgac caattctggg tcaagggctg aagttggttc
    12301 gtctaagagc aaaacatctg gtttcatagc aagcgcacgc gctagggcaa cccgttgctt
    12361 ctgtccacct gataaacggc gaggataatg gttttcacgg tccgaaagcc caaccttagc
    12421 caactcttcc ttggcaatct tagtcgcttc ttggtcagat aatttcttga caacaaccaa
    12481 gccttctttc acattatcaa gtgctgttcg gcgttcaaac aaattaaact gttggaaaac
    12541 catagacaac ttacggcgta gggcaaggat ttcttcttga gtgattttag aaaaatcaac
    12601 tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt tcaagataat tgagactgcg
    12661 aagaaaggtt gattttccag ctcctgaaga accaatcaag gctacgactt ctcctttttg
    12721 aatatccaag ttcagatgat ccaagacagt ctgtcctgaa aaggatttgc ttaaattcga
    12781 aatcttaatc attaacgaag gtctcctttc acatctgttt gcactgtatc aggtgcagaa
    12841 atagccattt ttctctcgat gaaacgaccg aggctttcaa ttccgatatt gactacccaa
    12901 taaacaaggg caacggagat gaagcgttca aaatagcgat aatcagctcc acctagaatc
    12961 tgagcttggg caaagacttc cacaacaccc gcactaaaag ctagagatgt tcccttggtc
    13021 aaaccgatga gggaattaat caaggttgga gtagctacca ccgctgcatt aggaataatc
    13081 actcgtcgat aaacttgcgc tcgggtcata cccagactgc gtgccgcctc aatctcacca
    13141 ggattaactg agagaatggc tgcacgaatg gtttcactag cataagctgc ctcattaaag
    13201 gcaaaggcga caatcgcaaa agctgcagct ggaatcgcat tgatattgag accagttccc
    13261 cattgctgat tgagggcttt caaagccaaa ggaattccgt agtaggtcaa catgagttgc
    13321 accaaaatcg gtgtcccttt taagaaacta acaaagaagg cctgcaaggg atataaaatc
    13381 ttgacacgat tgatcttcac aatggcaaaa agaagcgcca aaaccaagcc aaaaagggca
    13441 ccgccaattg tcaacataat tgttgttgga agttgttgga caattctagg gattccatca
    13501 aagaccgaac gtaggctaaa cagcttgcca tccggaatca attgcatcaa gttttggtac
    13561 caatctgatg ctaaaatcgt tgtaacattc ataaaaacat cctcttctga taatgattca
    13621 ttctaccatg cttctgccgt caatgaaatt atttgctacg gtcagataca gactttgtct
    13681 acctactagt ttatcatact ttgaaacagg gaagttatat attttatcta tcaaagagat
    13741 agataaaaac tatttcagtc ccagttcttc ataagatttt cgataaccga tttgcttaac
    13801 agttccattt tctactaaaa tgggccgttt taacaacata ccgtcacttg ctagcaactc
    13861 agccgcttct tggtttgaca aacttcctac cttatctttt agccctaatt cacggtattt
    13921 gataccactg gtgttgaaaa attgcttcaa ttcaaatcct gaggtttcta gccaattcaa
    13981 aatgacttct tggctaggtg tttcttccac gatatggacg gctttatagt ccacacctaa
    14041 ttgatttaat tcttgttttg cttttttaca agttgaacat ttggggtatt cgataaattc
    14101 taacatgtgc ttctccttct attgtttcta tctttaaaat ctactccttc atgctccaag
    14161 agccaagctt tcttttccac tcctgcagca taacctgtca gacgcttgcc tgctcccaac
    14221 acacgatgac aaggtactag gatagaccaa ggattgcgtc ccactgctcc accaattgct
    14281 tgagcagaag ccacttgcag gtcttgagca atttgtccat aggtcactgt ctgaccataa
    14341 ggaatgccct gtaaatagtc ccaaactctc ttttcaaaat tcgttccgat tggcgccaag
    14401 agcaagttgg ataaatcctg aggcttgcct ttaaagtaat catctaagca agcaataact
    14461 gggtctaaaa taggatgact aacaacttct tctatcgttt catctcctag tcccctctca
    14521 aaatgcttct gctcctgaac ccaaattcca tacaaataat ggtcatcagc tacaagtgat
    14581 agaataccaa ttggtgagga gtagaggatt tttacatact tcttttgcat tatttcttta
    14641 atttttgata ggccaagcgt tcgccatcta ctggcacctt accaacttgt ttaaaaccaa
    14701 gtttttcaaa aatatgttgc ataaccttgt tttcagcatg cgtatctgag cgaaaatcaa
    14761 gataatcaaa accttcaatc aagccctcta agaaggtttg agcaactcct tttccctgca
    14821 catctgctgc cacagcaata cggtgaaaga ctagatactc tgactctccc gcttgccagt
    14881 ttccctcata aatagcttca taggcctcct ctggactctt ggtcacagca gcataggcta
    14941 gcagttctcc ctcttccaag gctacgtagg cttgccctga gatgatatca tcgataataa
    15001 catctgcatt tggatagcca ttttgccact ggtcactacc agcatctgct aaacattttt
    15061 tggcatcctc catcacctgc atgatggcat ctacttcatt tggaaaagct aaacgaattt
    15121 ccatcttttt tcctcatttc tgactagttt ctcccatcat agcataattt aagtatttct
    15181 acaagcagtt aaaacttgac tttgtgtttt tgttatttta taatctagtt atcaaaacta
    15241 tataaaaagg agttgaaaat gaaaactacc ttttcctacc caaaatgggc agaaattcca
    15301 aacattgacc tctatctgga ccaggttttg ctctatgtca atcaggtctg cgcccctatc
    15361 tctcctaata aagacaaggg cctaacagca tctatggtca acaattatgt gaaaaatggt
    15421 tacctgacaa agcctgacaa aaaaaaatac caacgccaac agattgcccg tttgattgct
    15481 atcacaaccc tcaagtctgt attttctatt caagaaatag cccagacact taatactcta
    15541 caaactcaag caagttcaga ccaactctac gatgcttttg tggactacat gaaccaagga
    15601 attgacccag ctaaccctat tatccaaacc agctgccaaa ccgttaaact ctatcatcaa
    15661 actctagact taatcgatca tactcaagag gaggtaatcc aatgaacact agtcttaaac
    15721 tcagcaaaca actcagtttt ggagaggaga ttgctaatag cgtgacccat gctgtgggtg
    15781 cagtcatcat gcttatcttg ctgcctattt catccatcta tagttatgaa gcacacggat
    15841 ttttatcctc tatcggcgtt tccattttcg tcatcagtct ctttctcatg ttcctatcat
    15901 ccaccattta tcactctatg gcctatggtt cgacccacaa atatgttttg cgaatcattg
    15961 accattctat gatttacgtt gccattgccg gctcatacac gcccgttgtc ttgaccttga
    16021 tgaataactg gtttggctat ctgattattg tcatccaatg gggaacgacc atctttggta
    16081 ttctctataa aatctttgct aaaaaggtca atgagaaatt tagccttgct ctttacctga
    16141 ttatgggctg gttggttctg gctatcattc ctgccattat caatcaaacg acacccgttt
    16201 tctggagtct catggtaact ggcggactct gttatacagt tggagctgga ttttatgcca
    16261 agaaaaaacc ttatttccac atgatttggc atctctttat cctagctgcg tccgtactcc
    16321 aatacatcgc tattgtttat tacatgtaaa aaagttgaga aattcaatct caactttttt
    16381 ctttacacat attgataaag tactggtgca agcgcacatc atcagtcaat tctggatgaa
    16441 aagaacttac caacatattt ttttcttggg ctgcaacaat ttgattgttc actgttgcta
    16501 gaatttctac accctcacca acactactga taatcggacc acggataaag gtcattggaa
    16561 tcttgccaac tcccttacat tctgcttccg tgtagaaact tcctaattgg cgcccataag
    16621 cattacgctc gaccaccata tccatagttc ctagatgact ctctttctga gaagtgattt
    16681 ccttagccag caaaattaag cccgcacagg tcccaaacac tggtaagcca gatagaatgg
    16741 cttctcggat gggaagtagc atgttctggt cacgtaagag cttgcccatg gttgtagact
    16801 caccaccagg caaaatcaaa cccgacaagt cactctgatc ttgctgaaaa tcatctagat
    16861 ttctgagttc tacactctcg acacctaatt gatctagcac ttttgcatgt tctgcaaagg
    16921 ccccttgcaa ggccaatatt ccgattttca tctattttcc tcgttcagcc atgaggattt
    16981 ggatttcatt ttcattaata ccaaccatgg cttctcctaa atcttcagag atttgagcta
    17041 ggatttgagg attacggaag ttagtcacag ccttaacaat ggcactcgct cgtttaacag
    17101 gatctcctga cttgaaaata cctgaaccga caaagacccc ctctgcccct agttgcatca
    17161 ttaacgcagc atctgctggc gttgcaacac ctccagcagc gaaatttaca actggcaatt
    17221 ttccatgttc atgaacatat tggaccaatt ctacagggac ttgcaaatcc ttggcagcaa
    17281 cataaagctc gtcctcacgt aagttttgaa tgcggcgaat ttcctgattc atcatacgca
    17341 tatgacgaac agcttggact atatcccctg tccctggttc tcctttggta cgaatcatgg
    17401 aagcaccttc agcgatacga cgcaaggctt cacccaaatc cttagcacca cagacaaaag
    17461 gaacttggaa ttctttcttg tccacatgga aacggtcatc agctggagat agaacttcac
    17521 tctcgtcgat ataatcaatt tcaatagcct ctaaaatctg agcttcaaca aaatgcccga
    17581 ttctgacctt agccattact ggaatactaa ccgcttcttg gatttcctta atcatctttg
    17641 ggtcgctcat gcgggaaact cctccagctg cacgaatatc agccggaatt cgttccaagg
    17701 ccatcacagc tgccgcacca gcagcttctg cgatacgagc ctgttcagga ttctgcacat
    17761 ccataataac accacccttg agcatctgtg ccaagttttt atttagttca taacgatttt
    17821 cagtcatttg ttttatcctc atcatttgta ttggtagcta ccttttcatt ttaagtcaga
    17881 acagaatgaa gcacaagata aaaaaactat aattgtatgg ggacaaatta gctaaaaact
    17941 atctgacctt aacttaaggc cagatagctc attcattttt atttttcagc cgtaagggca
    18001 gccattgtga tgtagttgta tggtttgttg aagtgtggca agaagaagag gtctgtcaat
    18061 gccaatttat caattgtcac atgctcttgg atagcaagtg agaacatgtg gattcccatg
    18121 ctaattgcaa tatcatgtga aaccatttgg gcaccaagaa tttcacggct atctttgtca
    18181 aagacaatct taattgctac ttcatggttg tcatgtttca tgaattctgg tttttgaaga
    18241 tcgttaaagc ctgtttcagt tgcgttgtaa ccagcagctt tcgctttttc aagagtcaaa
    18301 ccagttgaaa ccatgtgaag accgtagatt gagataccat ttgaaccttg aacaccgatt
    18361 ccttccaatt catgtccaca agcattgtag gcaccaacga taccagtgcg aacagcattt
    18421 gaagcaagag cgatatagct tgtatcttta cgagcattgt cataaacagt cgcacagtca
    18481 ccaacagcgt aaacgcctgg gatagatgtt tcttgtttct tgtctacaag gaaggcaccg
    18541 ttgcggaaga gttcgatctt accatctgca agggctgtgt ttggacggaa accaactgca
    18601 aggataacca tatccacgtc aaagctttct ttgtcagtaa tcaagcgttc aactttaccg
    18661 tcaccttcga ttgctttaac agtttgacct agagccaagc ggatgttgtg atcttccaag
    18721 ttcttcgcca tcatttgtgt gaagtctttg tcatagtaac cgttcaagac agtatcaacg
    18781 atatcaacaa ggacaacttc ttttccaaga cgttcaaagg cttcagcaag ttcaacaccg
    18841 atgtaaccac caccaacaac ggcgatacgg tcgaggtgtt ggctcttgtc agaaagttta
    18901 ttgataactt cttcagcatt ttggtacaat ttcacgaatt gtacgttttc aagagttgct
    18961 ttaaattcgc ggtttccttt aacaatttca acaccttcga ttggtggcaa gattggtgta
    19021 gaacctgtag cgaaaatcaa tttttcgtat gattctttgt gctcttttcc ttcaacttcc
    19081 gctgtaacta ctttgttatc atagtcgatt gaaagaacag gtgagttcat gtaaacttta
    19141 gcacctttag cttccaattt ttctttatca gaatagaaca agccttcagc accgtcaatt
    19201 tgttcaccaa tccaaagagc cattccacat cctaggaaag agatgttaga gttttggtca
    19261 aatacaacaa tttcgttctc atttccaaaa ttatccaaca tggtattgat acatgctgta
    19321 ccagcgtggt tagcaccgac tacaacgatt ttactcataa aaatttcctg ccttcattct
    19381 aaatttacct tactagtata ccatttaccg ttagcgttta caaggggggt actattattt
    19441 cagcattttt tatcaaaaat aattccccat ttatcctgtt ttcaagcctg atgaatttta
    19501 tgagcatatt tcttgacttt ttccaaattt tgtttgtgaa aattctaact ttatggtatt
    19561 ataataacat gaaaatgaaa tgtaagggct tcaatttaga tgattatttg atattacaac
    19621 ttattttcta taagagaaag gagttggtag cttgactctt cgttcacatt ctgaacggct
    19681 aatggggact actatcacta tttcattagt ggatgagcaa gccgatatct ttctccaaaa
    19741 atcctttgac ttgctcaaag aacttgaata ccgcttcaac gccaatagtc aagaatctga
    19801 gttgatggaa atcaattatc aagcaggaat atcccccgtc acggttcatc cagacctgtt
    19861 tgagctgatt tcacttgggt tagagcatag cctagcgctc tctagccacc tcaatatcag
    19921 cattggtccc ttaattcaaa cctggcgtat cggtttttca gatgccaagg tcgcccagcc
    19981 tcaagaaatt gaatcggtgc tgcctctaat caatcctcat ggtatcgagt tagattcttc
    20041 tacttctact gtgtttttaa aacagaaagg aatgaaaatc gatctaggtt gtttagccaa
    20101 aggatacagt gcggataagg ttgcccaatt tcttaggaaa gagggggtga cttctgcctt
    20161 gatcaatctg ggagggaata tcctgaccat tggaaaaaat caggcaagag gggataaccc
    20221 atggcaaatc gggattcaag acccagccaa tcctagggga aatcatttaa tgaccatccc
    20281 tgttgtcaat aaatctgtcg taacttcagg catttatgaa cgtcacctga ccgtcgatgg
    20341 acaagattac catcacattt ttgacagtca aacaggatat cctgttgaaa cggaactagc
    20401 gagtctaaca atcatctctg ataaatcagt cgatggcgaa atctggacga ctcgactctt
    20461 tggagaaaga cctgcttcta tcctctggca agtcgaaagt ttggagggca tcgaagtcat
    20521 cctcatcgat aaagaaggcc acctaagctg ttcttcagga attcctactc tatagagaca
    20581 actgtttcaa tggaatttta aaattgtatt atgtaaaaag agaaaggaaa tctcatgtta
    20641 aaacttattg ctattgttgg aacaaattca aaacgttcta caaaccgtca attgcttcaa
    20701 tacatgcaaa aacactttgc tgacaaagct gaaattgaac ttgttgaaat caaggccatt
    20761 cctgtcttca acaaaccagc tgacaagcaa gtacctgctg aaatattgga aattgctgct
    20821 aaaatcgaag aggcagatgg cgttattatc ggtactcctg agtatgatca ctctattcca
    20881 gctgttttga tgagcgctct tgcttggttg tcttatggta tttacccact tttgaacaaa
    20941 ccaatcatga ttacaggtgc ttcttacggt acgcttggtt catctcgtgc ccaattgcaa
    21001 cttcgtcaaa tcttgaatgc tcctgaaatc aaggcaaatg ttcttccaga tgaattcttg
    21061 ctctcacact ctcttcaagc atttaaccca agtggcgact tggttgacct tgatgttatc
    21121 aagaaattgg atgccatctt tgatgacttc cgtatcttcg tgaagattac tgagaaattg
    21181 cgcaatgcgc aagaattgct tcgcaaagat gcagaagaat ttgactggga aaatttgtaa
    21241 gataggagac cgaaagaatg aaatttgttg gacttgttgg atcaaactac gatcaatcat
    21301 ataaccgcaa actcttggaa tttatccgtc gcaatttcaa attcaaattt gaattagaag
    21361 tacttgaaat cgacgaagtt ccaatgttta accaagacga aaaatgggac gaaagcttcc
    21421 aattgcgttt cttgtataac aagattacac gtgctgatgg tgtcattatc gccactcctg
    21481 agcacaacca cactatctca gcttcactca aatctgtact tgaatggctt tcatacgaag
    21541 ttcatccatt tgaaaacaag cctgttatga ttgtgggtgc atcatactat gaccaaggaa
    21601 catcacgtgc tcaagttcac cttcgtaaaa tccttgatgc tccaggtgtt aatgcctaca
    21661 cgcttccagg taacgaattc cttcttggta aagctaagga agcttttgat aacaacggaa
    21721 atatcaccaa cgaaggcact gttaaattcc ttgaaacttg cttagataac tttgttaaat
    21781 acgtaggagt cgtttcgaaa ttgaaaaaac caaaaccaat cgaatcagaa gacttggatt
    21841 gtggaaaacc aatcgctaca accatcacag aagttgaccc tgacgatcca gaatgggtag
    21901 aaaaagttgc agcaatcact ggtgctgttt ctggtgatac ctatgtcaaa ttggaccacg
    21961 gtatcctaac agttaaccaa attgatatgt tcttgaaagc tatgccattt gaattgacat
    22021 acgctgacga caacaaccaa ttcctctact acaacaacgc tcaccaagat ccagacacta
    22081 tgttcgctaa acgtgtacca cctcaatcag gtagccgtat gtcgactgtt catggttctc
    22141 ttccaccagc acgtatgaag aacgtagagt gggttatcgg aacacttcgc aacggaaacc
    22201 aagaatacgt ccgtacgatc gttccaggtt ctcctgcagg tgttatcaac actcacaact
    22261 accaagcaat gtactatcct gatggatcat acgctggtat caatgaaatc gtctttaact
    22321 tccaaccatg gcttgactgg tacctaaaag agactggtca acgtttggtg ggtggtagcg
    22381 gaccatttgc tcctgctgcc ggaagtcatg gagacgccga tgctacttct ggcgcttctg
    22441 attcagggga tgctggaggc cacggtggtg acgcagacgc tacagccggt gcaagttact
    22501 aatgatagtt tttaggaacc attatggttc cttttttagc gaaaaaagac tagcaaacac
    22561 aaactgctag tctctttgtt atcgtttcat ctatctttgt taaaagaaag aatacatatt
    22621 atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt acttggcgta
    22681 gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct tcaatgtggt
    22741 gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct tgttccactt
    22801 tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg atacgagcaa
    22861 tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta tcaaatttta
    22921 tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta gttgtgacaa
    22981 gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag tattttcaaa
    23041 gaaagcatca atgactgggc taagcgcaaa gagttgtttc aattgctgac ttgcaggtcc
    23101 tgataaaacg agtgtttcta ctgcttctgc caaagctttt tcttggtcat tctcaaatag
    23161 tgctgaatca accgtagcaa ccccttctgc cttctcagcc aggttaaagg cacgagaaag
    23221 tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag cacttgctgc
    23281 ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttctt tgatatcttt
    23341 tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa cctctgcttt
    23401 attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca gctcatccat
    23461 agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg cacgacgaag
    23521 ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac tcaaaatcgt
    23581 atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa gttctccttc
    23641 agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag tttcaccagc
    23701 aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac caaccatacc
    23761 tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg tttcatccac
    23821 tgacaaacca gctttttctg ccaaaagtac agtgatttga cccgtacgaa tcatgtgttc
    23881 acgaagggaa ccaatcttct catggaaggt gacattgttt aatttttcaa caagatctga
    23941 aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc gggctaccaa
    24001 gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt tacgaacaga
    24061 aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac gctggtgttc
    24121 cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat caaaacttcc
    24181 catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat ccgcatcaat
    24241 ttcgatatgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa tcatttgctc
    24301 acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat agctcaatgc
    24361 tgactgaatt ttggtttctt tccccaagaa acgatggcca cggctcacac gacttccctt
    24421 gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta aagtgtgaac
    24481 agggcggatg tattcaaagc tatttccagc ccagtgcatg ctgacaggga aagtcaatga
    24541 cttcaagaca tctacaacac caggaacaat ggcttcaact gattggccaa cttcttcctt
    24601 agtgacatag acatattctt cacccttgat ttcacggaat tcgatatctt caacagtcaa
    24661 acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac tatccaaggc
    24721 aattttcttt gctggtccct tgaaatcttc tgtcaaatca gactgtttgt ctgataaacc
    24781 agtcacacga acagccaaac gacgtggtgt tgagaaggtt tgaatggctt caaaagacag
    24841 gcggttttcc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac ttggtgtgac
    24901 aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg tcatgttttt
    24961 tcctttacat tttcttcaat cttttttgat atgcacctta ggtactgggg acgtcgctaa
    25021 ctaactctgt gagtctgtat ggaaatccaa tgctaaattt atcaagattt ccaacaatct
    25081 catcaaagtg gatttagctg actgattttt gaacctaagg tttcaaaaat ccccacataa
    25141 cagtacggcg gtgcatatcc ctctagcaag tcttcgactt gcctctaacg attttagagt
    25201 acagtattac tcactattct gcgtcttctg ctaaaagttt aactcgtgtt tcttcatcca
    25261 aaagtgggta acctaggcgt ttgcgttctg caacaaaggt tttggctaca acacgggcca
    25321 agttacggat acgagcgata tagcctgcac gctctgttac agatacggca ccacgcgcgt
    25381 caagcagatt aaaggtatgt gaacatttga gaacatagtc ataggcaggg tgtaccaagc
    25441 cttcttctaa tgcacgacca gcttcttttt caaacttatc aaagttttca agcaacattt
    25501 cttggtccga aatttcaaat gaatattttg agtgctcata ctcaggctgg ataaagattt
    25561 ctccgtattt tacaccatca gcccactcga tatcatagac agagtctact tcttgaatgt
    25621 aagaagccaa gcgctccaaa ccataggtaa cttccgcagt cacagggcca gttgccaatc
    25681 caccgacttg ttggaaataa gtgaactgag tgatttccat tccgtcaagc caaacttccc
    25741 aaccaagacc agctgaacca gttgatgggt tttcccagtt gtcctcaaca aaacgaatat
    25801 cgtgctccaa aggattgatt cccaattttt ccaaagattc aaggtaaagt tcttggatat
    25861 ttgatggaga aggcttcatg accacctgga attggtggtg ttggtagaga cggttagggt
    25921 tttccccata acgaccgtca gcaggacgac gtgatggctc tacataagct gcattccatg
    25981 gctcaggtcc gatagcacga aggaaagtgt aaggactcat tgttcccgca cctttttcat
    26041 tatcataagc ctgcataagc atacaacctt ggtcattcca aaattgttgc aaagtaagga
    26101 tgatttcctg gaaagtcaat ttcttagaca ttgtttactc ctttttatat aaattacttg
    26161 cgacatgagt ctgcctcgtc gatcatacga ggcaagacga gttagtactg cgtctagctc
    26221 aggcactcta gtgctgagcg tggggcagtc cgactgtaag gaggtttctg ccactgacgc
    26281 agtgaaatgt caatttctta gacattgttt actcctttag tttttatatt tctttttcaa
    26341 ttaatttctg ctgaacaaac caatctaaca agggaaaagt tttttctgta tttgcccata
    26401 aatgataaga atctaagacc gcttgtacac tacctaaatt ttctaccaac ttatctactg
    26461 tcaaaaagct gcgccagtat tcataaaaaa tacttgcata gttcttatca taagtcgaag
    26521 aaccaaaatc attcaatgaa tgccaaccat acttcttctg aaaaagttct acgagagatt
    26581 gattacaaat tttttccgct tgaaattcct cttctgtcaa gaaatacttg cgactaatat
    26641 attcaaccat cccctcttcg aaccagatat aagagtcata accatcaaaa tcatctaaaa
    26701 aatgctccga ccaatgagct aactcatgtc ctacaatctg taagaggaaa ttttcagata
    26761 aagaatgata gtgactttct attgcttgag tttgctgaga acactcgtag tccatcaatt
    26821 gatacaaata caactctttc caaacagcta aatcaggcgc cataaccatt cgtctattat
    26881 ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta aaatctgacc
    26941 aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa gattgataat
    27001 ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt tgactatcgt
    27061 cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag actgaataag
    27121 caaaagaacc gcgtccgaca atcctaggtt gatttaacct aggggcgtca gaaacgcggt
    27181 tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc cagctcatta
    27241 ttttgcccta cttggctccc actatcccaa gctcgcttga agaacgccat aagactttct
    27301 ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta tgattttcta
    27361 gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt ttaagttttc
    27421 tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa tacgagaggt
    27481 tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccaggcca aggctatctg
    27541 agcaatcgat tttccgtgat ttgctgcgat ttcttgcact tgcttgctat caaatagttc
    27601 tccctgacca aaaggccccc aagcttccaa taaaattccc ttttcacgac agtaagctac
    27661 gacttgatct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag gaacgatagt
    27721 tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc cgatagcacg
    27781 gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat tgcgagtttt
    27841 ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat acaaatccaa
    27901 ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt gctcataggt
    27961 ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa ctccgctatc
    28021 tttgattgct tgaccaacac ttttttcatt ctgataaatc gccgccgtat caatatgacg
    28081 ataaccagcc ttcaaggctt ctaacactgc acgataggct tcttctccat ccttagcctt
    28141 aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat aggtattcat
    28201 atgcttctcc ttctatttta ttcatcaatt atagcataaa acatttttta agtagtacta
    28261 tcttctgtaa aaaataagtc tgtcaaatat ctaacagacc tattttttct tgcttattca
    28321 tcacgactat agtacagctc atgagctttt aggcgagtat tgagcatctc tggtatggtc
    28381 acaaaggtat aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc attgactgtc
    28441 ggactgtgaa tatcatgcat caaaacgata gagccgttag ctacttgatg ctgaatttct
    28501 gtcaaaatag atgcttcatt tttactcttc cagtccagac tatccacatc ccacatgata
    28561 aagctcaaat ccaagctatt gcgaatatca tctgtaatag caccataagg tggacgcatg
    28621 agtttagaac tagaacccag cactttagtt agcacatcct cagtatcagt aatctgcttt
    28681 ttagcttcat caagagagag ttgcgagaga atcggatggc tccaactatg gtttccaaca
    28741 acatgacctt cagattttat cctcttcacc aagtcctcat tcccagaaac atttttccca
    28801 agcacaaaga aagtcgcttt aatatcatat ttagctaggg tctctaatac ctgcggggtc
    28861 gttgctggat ttggaccatc atcaaaggtt agagcgacaa ctttttgata tttcttatca
    28921 aagtaagatt ggtacaaggc cgcatctttt tcgagtaagt acgaagattg gataacatca
    28981 aagaaagcag atactggcaa ggctatctct tctaaatttt caaccacagg acttggataa
    29041 aggataatct gactatcctt gtaatcaaaa ttccatgcag acaagtcttg gtcagagaag
    29101 ttttttacaa tctgctcact ctggtcttgc tctatttttt tatcctctat gaaggaggtc
    29161 aactctttta tcagctgttc cttagcctta ctagcatctg aaaacagttg gtcaagtgta
    29221 aaaggttggc catcttctgt taagtgaacc tttcctagac tggtcttttc agtctcttca
    29281 accttaaaag caactaaatc atagatttgt ttggtcacat tacgattaac gactcccttt
    29341 aaacctgact cttcttgctc tgtatagtag aaaacaagat tgtccttact ttctagcttg
    29401 tccttaacat cttgatttat caactcccta acagaggaaa tcactttctc cccttggaga
    29461 ggataatagg caatcacttc ggcttgcccc tgacgaaaat gctccttctg atttccctca
    29521 ctcaattgat catctttctc ttttttgagc gattcaatct tttgctcaaa acttttttgc
    29581 tggtagatct taaaagcaat aaatccgcct aataggcaaa tagaaattgc taaaatacca
    29641 atcaaagcca ataatacatg tctcgttttc ccgtgtctgc cacgtcctag tctactttta
    29701 ttcatattta tatcataaca aatttgagca ataatttcaa tgatacaatt tggcaatcca
    29761 caattcctct agcaagccaa agcatcttac atcaactcca acatccgtat ctgtagagat
    29821 tatgtagttt tctatctatg gaatttctag tagattgaag ctagaatagt agacctatac
    29881 ttctaaaaca ttgttagaaa tcgatttgat tgtcctgatc gatttgtcct gttctcattt
    29941 cattttacta taaaactatg aaaagctagg tcatctgaga agtaattact atcgacaatt
    30001 gcgatagcgt atactggtgc gatttgtttg tagctctggt gggtatcacc ttcacgttgg
    30061 cgaatttttt ctaggttttg attaacctga ctgcacagat aagcccatag gcgattgatg
    30121 aaaaaattct gatgatgaac ctgaatttcg atgataactt ggattccatt atctaactcc
    30181 gccaagacgt ctatactagt atagaaatct tgcgctgagt agggcatgga aggcaagacg
    30241 tgaatgttac ttccctccaa aatcgtcaca ttttttgctg gcaagtccag catatcgcgg
    30301 ataaattgac aaatcattag ttgggctgat acctggatgt ctgagaatcc tccttttcct
    30361 ccttctatta tagcacattt ttaaatctag gtttgctaga ttctatgctt ctatctattt
    30421 attcggaaaa gaaagctaat agcttatttg aaattttctc ctctctgttg gatagtcccg
    30481 tctatctatg ttataatgaa agaaggattt aaaatcggaa aaggagtatt tatgctaaaa
    30541 ttaggtgtca tcggaactgg cgctatcagc catcatttca tagaagcagc ccataccagt
    30601 ggagaatacc aactggtcgc aatctattct agaaaactag aaactgcagc aacctttgct
    30661 tctcgctatc agaatatcca actattcgat caattagaag tcttcttcaa atcttctttt
    30721 gatttagtct atatcgccag tccaaactcc ttacattttg ctcaggcaaa agctgccttg
    30781 tctgctggta aacatgttat tctcgaaaag ccagctgtca ctcaaccaca agaatggttt
    30841 gatttgattc aaacagctga aaaaaataac tgttttatct ttgaagcagc tcgtaattac
    30901 cacgagaaag cctttactac tatcaaaaac tttttagcag ataagcaagt gttaggagca
    30961 gatttcaact atgccaaata ctcttccaag atgcctgact tgttggctgg acaaacacca
    31021 aatgtctttt cagaccgttt tgctggtgga gccctcatgg atttggggat ttatcccctc
    31081 tatgctgctg ttcgtctttt tgaaaaagct aatgacgcga cttaccatgc tcaacagctt
    31141 gacaatagca ttgacctaaa tggagatggt atcctcttct acccagacta tcaagttcac
    31201 atcaaggctg gaaaaaacat cacttccaat cttccttgcg agatttatac aacagatgga
    31261 accttgactc tcaacacgat tgagcatatt cgctcagcta tttttaccga ccaccaagga
    31321 aatcaagtcc agcttcctat ccaacaggct cctcatacga tgactgagga agtcgctgca
    31381 tttgcacaca tgatccagca accagatctg aatctctacc aaacttggct gtatgatgca
    31441 ggctctgttc atgagctact atataccatg cgccagactg ctggcattag atttgaggca
    31501 gaaaaatgaa aaccaaacta ccgactgaat ggcaagaact gagtgaccaa ctcggtttcc
    31561 aagaattcac ccccattcaa actcaactat ttgagcccct tcttgctgga gaaaacctcc
    31621 taggagtgag ccaaaccgga actggtaaga ccctagctta tctcctacca agtcttctca
    31681 gactacaaaa gaaaaaagcc caacaactct tgattctagc accaaatacg gaacttgctg
    31741 gacagatttt tgatgtatgt aaaacgtggg cagaagctat cggattgact gctcaactct
    31801 tcctttcagg ttcaagtcag aaacgacaaa ttgaacgtct taaaaagggg ccagaaattc
    31861 tgattggaac tcctggccgt atctttgaac tcattaaact taaaaaaatc aagatgatga
    31921 atgtggaaac catcatcctg gatgaatttg accaattact agatgattct cagattcact
    31981 ttgtagaaaa aatcactcac tacgcacctc gtgaccacca actggtctac atgagtgcca
    32041 cgaccaagtt tgatcaagaa aagattgtac cgaatacacg cactattgat ctctctgatc
    32101 aaaaattgga caatatccag catttctaca tgcaggtaga ccaacgtcat cgagtggata
    32161 tactacgaaa actggctcat gtagaggatt tccgtggtct agtcttcttc aatagcctgt
    32221 cagaccttgg gaatgccgag gaaaaactac agtatcgtga tatattggct gtttccctcg
    32281 ctagtgatgt taatgttaaa tttagaaaag tcatcttaga aaagtttaaa gataaccaac
    32341 taaccctgct tcttgcaact gaccttttgg ctcgtggaat tgatatcgat agcctagaat
    32401 gtgtcgtaaa ctttgatatt cctagagata gcgaaaccta cacacaccgt gctggccgta
    32461 ctggccgtat gggcaaggaa ggatatgtta ttactctcgt cactcataca gaagaaatca
    32521 aaaaacttaa aaagtttgca agtatccgag aaattgtcct aaaaaaccaa gaactctata
    32581 tcaaataaag ccccattgct ctatacacat ccagtgtatg gagctttttt acaagcaatt
    32641 caattttata ctcatctgtt ttcaaaaagc attctaatcc atctccgatt aacgatggac
    32701 tttatcacct ccttctccag ttcttgtata atatcttgaa gttgattcat gacatcttcc
    32761 aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttgttca
    32821 acggggttta tctctggtgt gtatggagga ataaatgcaa agccaatatt agtcggaatt
    32881 tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag ataatcatct
    32941 ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc actcagtatt acatccacca
    33001 gctcttaaga aaaatgattc gcctgtatgg gcatcaacag ctccataaca atagcgaaat
    33061 tctcgtatat agtgactatg gacatgtgga cctactccta ttggagacca acaagatccc
    33121 agtttactga ttctaccgaa accagcctca tcttggtaca tcgagcgaac cttatgaaaa
    33181 cgtctactgg ttttaaagcg ctttcttttc ttcttgaatt gagattttat ttttagacgc
    33241 gacaatggtt tgagcgtctg ctttcttagg atgttctgga cgtggcataa tatttcgcca
    33301 accatggcgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgat ataagcctga
    33361 aataaggtat tgtaacaaat tctcctgcct ctgtagcctt caaatggcgg gcaagaaagg
    33421 ctttctcttc ctcaactgtc atatatgcat ggttacgacc accacgtgtt tcttgaagga
    33481 gagagtcgag tccgaactcc t
//
LOCUS       DAVHIU010000009        32367 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVHIU010000009 DAVHIU010000000
VERSION     DAVHIU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.009"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(116..1750)
                     /locus_tag="VVN06_000351"
     CDS             complement(116..1750)
                     /locus_tag="VVN06_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561960.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENLDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1731..2480)
                     /locus_tag="VVN06_000352"
     CDS             complement(1731..2480)
                     /locus_tag="VVN06_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561961.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2659..4113
                     /locus_tag="VVN06_000353"
     CDS             2659..4113
                     /locus_tag="VVN06_000353"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU5561962.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4203..6821)
                     /gene="alaS"
                     /locus_tag="VVN06_000354"
     CDS             complement(4203..6821)
                     /gene="alaS"
                     /locus_tag="VVN06_000354"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5561963.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVTQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6843..7328)
                     /locus_tag="VVN06_000355"
     CDS             complement(6843..7328)
                     /locus_tag="VVN06_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU5561964.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7603..7842)
                     /locus_tag="VVN06_000356"
     CDS             complement(7603..7842)
                     /locus_tag="VVN06_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5561965.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILSHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8087..9157)
                     /locus_tag="VVN06_000357"
     CDS             complement(8087..9157)
                     /locus_tag="VVN06_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5561966.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9154..9927)
                     /locus_tag="VVN06_000358"
     CDS             complement(9154..9927)
                     /locus_tag="VVN06_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5561967.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9924..10730)
                     /locus_tag="VVN06_000359"
     CDS             complement(9924..10730)
                     /locus_tag="VVN06_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5561968.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10711..11868)
                     /locus_tag="VVN06_000360"
     CDS             complement(10711..11868)
                     /locus_tag="VVN06_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5561969.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11927..12877)
                     /gene="murB"
                     /locus_tag="VVN06_000361"
     CDS             complement(11927..12877)
                     /gene="murB"
                     /locus_tag="VVN06_000361"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5561970.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(12989..13741)
                     /locus_tag="VVN06_000362"
     CDS             complement(12989..13741)
                     /locus_tag="VVN06_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU5561971.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLVYIIYGLAMAAFFEETARLVFFKWLEKKI
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLIAYGTKEIFCKKS"
     gene            complement(13751..14482)
                     /gene="budA"
                     /locus_tag="VVN06_000363"
     CDS             complement(13751..14482)
                     /gene="budA"
                     /locus_tag="VVN06_000363"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU5561972.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14624..15895
                     /locus_tag="VVN06_000364"
     CDS             14624..15895
                     /locus_tag="VVN06_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5561973.1"
                     /translation="MKRQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFMAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15915..16730)
                     /locus_tag="VVN06_000365"
     CDS             complement(15915..16730)
                     /locus_tag="VVN06_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5561974.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16887..17540)
                     /gene="phoU"
                     /locus_tag="VVN06_000366"
     CDS             complement(16887..17540)
                     /gene="phoU"
                     /locus_tag="VVN06_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5561975.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17552..18310)
                     /gene="pstB"
                     /locus_tag="VVN06_000367"
     CDS             complement(17552..18310)
                     /gene="pstB"
                     /locus_tag="VVN06_000367"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5561976.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18323..19126)
                     /gene="pstB"
                     /locus_tag="VVN06_000368"
     CDS             complement(18323..19126)
                     /gene="pstB"
                     /locus_tag="VVN06_000368"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5561977.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19137..20021)
                     /gene="pstA"
                     /locus_tag="VVN06_000369"
     CDS             complement(19137..20021)
                     /gene="pstA"
                     /locus_tag="VVN06_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5561978.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20011..20928)
                     /gene="pstC"
                     /locus_tag="VVN06_000370"
     CDS             complement(20011..20928)
                     /gene="pstC"
                     /locus_tag="VVN06_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5561979.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20928..21806)
                     /gene="pstS"
                     /locus_tag="VVN06_000371"
     CDS             complement(20928..21806)
                     /gene="pstS"
                     /locus_tag="VVN06_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5561980.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22380..23684)
                     /locus_tag="VVN06_000372"
     CDS             complement(22380..23684)
                     /locus_tag="VVN06_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU5561981.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCVSLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23685..24458)
                     /locus_tag="VVN06_000373"
     CDS             complement(23685..24458)
                     /locus_tag="VVN06_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5561982.1"
                     /translation="MESKFEFAKGLVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24448..24726)
                     /locus_tag="VVN06_000374"
     CDS             complement(24448..24726)
                     /locus_tag="VVN06_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU5561983.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24726..25127)
                     /locus_tag="VVN06_000375"
     CDS             complement(24726..25127)
                     /locus_tag="VVN06_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU5561984.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25337..25954)
                     /locus_tag="VVN06_000376"
     CDS             complement(25337..25954)
                     /locus_tag="VVN06_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU5561985.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETVQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(25944..26717)
                     /locus_tag="VVN06_000377"
     CDS             complement(25944..26717)
                     /locus_tag="VVN06_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU5561986.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     TGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26728..27465)
                     /locus_tag="VVN06_000378"
     CDS             complement(26728..27465)
                     /locus_tag="VVN06_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU5561987.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27470..28600)
                     /locus_tag="VVN06_000379"
     CDS             complement(27470..28600)
                     /locus_tag="VVN06_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU5561988.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28684..29073)
                     /locus_tag="VVN06_000380"
     CDS             complement(28684..29073)
                     /locus_tag="VVN06_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5561989.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29089..29472)
                     /locus_tag="VVN06_000381"
     CDS             complement(29089..29472)
                     /locus_tag="VVN06_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU5561990.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29473..30261)
                     /locus_tag="VVN06_000382"
     CDS             complement(29473..30261)
                     /locus_tag="VVN06_000382"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5561991.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30254..31189)
                     /locus_tag="VVN06_000383"
     CDS             complement(30254..31189)
                     /locus_tag="VVN06_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU5561992.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31319..31495)
                     /locus_tag="VVN06_000384"
     CDS             complement(31319..31495)
                     /locus_tag="VVN06_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5561993.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31635..32342)
                     /locus_tag="VVN06_000385"
     CDS             complement(31635..32342)
                     /locus_tag="VVN06_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU5561994.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
BASE COUNT         9686 a         7403 c         6205 g         9073 t
ORIGIN      
        1 tcaaaacatt cctccctata tttttgatta ccaatcttaa tcatttacaa ctacattcta
       61 acaaactata aaagcgtttg tcgaattgaa tttatcaagc aagcaaccaa ctatctcatc
      121 ttttttctat ttctgccaat atgcgtgaca ggtaataatg atagccaaaa atagcaagag
      181 caagcaagac gataagagct cctactccca agttgatggc aaggataggg gagagagact
      241 gaaccaagaa tatgctccca attacaaggg ccatcaggat tgcactataa ataaacaata
      301 aaactatggc gactatgcca tttgaacgat tcaccaggtc cgtaatgcta ctccaattgg
      361 ttgacagatt tttaacgtcc ttaaagtaat ggtggcaaga aaggatgaca ctggcaatga
      421 tccagactac aagaaggtaa atcatcgaaa ggatggacaa gcctagatat agagaaagac
      481 cgagcaaagt cagaattggt aaaaaggact gaacagcaaa gataatccaa aatttcactt
      541 tcacataacg agcaaagtca aagggtaaac tcttcagaaa atcaagattt tccctctcca
      601 aggacaaggc aattgaatgc aggctggtga tattgttatt gacaactgct ataaacactc
      661 ctacaaaaaa caagggtaac cagtatggag gatgaatgtc tggaactatc tgagaatctc
      721 ggattttgga aatcagactg atcatcatga gataaggaag gaaagcactt gtaaaaagca
      781 ctgtaatcac gccagtcccc tgtcccaaga gggtgaggtg gtagcgtaaa accatacgga
      841 aaaagccctt tttagtagtt gaaatcctct ccttgctgcg acgttctttt ttgaccttct
      901 cctcactgtt aagcaggatc acgtcataaa aacgaggaag gaccttcttt ttggtcaggt
      961 aaagcaagaa aacagttaaa gctatccaag cgagcagacc caatatggct tctattgaaa
     1021 aaggctccac tgctattttg taaaagatat gaagaggata aaggagaaat ggaatgtctc
     1081 taactttgtc aacaatactt ccaaaagtcg actgtagaaa gaagacaaat attaaaggta
     1141 tgagaactcc tatcccaatc atcacattcg aaaaaataga ctgatacttt ctgaagaccg
     1201 tagtttgagc caagaaatgc actgctacta ccatcactag agccacagag acaaataata
     1261 aggtcaagga cagtagcatc aaaggcaaac ccagccatag agaaggagct agcctaatgt
     1321 agaggactag aaaataagct aggattggta caattccagt tagagctggc aaaaggacag
     1381 acagtccttt agcaattata atctctgatt ctttaaaggc atagggccta tacgatacca
     1441 aatccttact ctcataaaag acattgtaaa aggccgttaa agaagttgaa aaggcaatca
     1501 ctagtaaaat agcaaccatc gagctaaaat aaataggtat ttcctcaaaa ggaaaatgaa
     1561 tggctatatt actaaaacag atgagcatca agagactgga aaaaatgtaa gaacttagga
     1621 ctctagcgga aacatttact tttttcccag gattcttagc ctgcttcttt cgtagattag
     1681 ccagattagc ttcttgagat gaatagagga tattgatatc aactaatttt ttaatgacct
     1741 tgagacgcat ccgcaacctc ctcttttcta ccagcaagac taaggtagat actttccaaa
     1801 gactggtctg ggtggtcttt cctcaagtcc tctaccttac cacaataaat caaatgcccc
     1861 tttttcaaaa tggcaatccg atcacagact tgctctgcca cctctaggac atgagttgaa
     1921 aacaagactg tcttcccttt ttgtgcatgt tccttcatca tctgtttcaa atcaaaggca
     1981 gcctggggat ccaaaccagt caagggttca tccaagaccc aaatatcggg atcagacaag
     2041 agtgctccga tgacaaagac tttctgacgc attccgtgag aaagagtttc aataacctga
     2101 tagcgatttt cagcaaaatc aaaaacgttc aatagcctag ctagactagc ctccaagtca
     2161 gatctactca gatcatagga tgaggcgatc aattcccaaa attcattggc cgttaagcgt
     2221 aaaaataagt caggcgagtc tgctacgtag ccaatctttc gtttaatagc caagcgattt
     2281 tccgataact cctgaccgtc taccaaaata cgaccactgc tgggtgaaat gatactgact
     2341 agggatttta tggtggtcga ttttccagcc ccattatgac caatcaagcc cataatctct
     2401 ccattttcaa tctgcaaatt gagattactc aaggcctctt tatcaccata caacttacta
     2461 acattttgaa attcaatcat gggacaactc ctatctttat ctgtattata catcgctata
     2521 ctagcacttt gatacaaaaa aatcaatact ttattttaag tagttaaaat agtttccttc
     2581 tatcttacgc aaacgtttgc gccaagagat actttatgat agaataaaga acaagattga
     2641 caagtaagag gaaacatcat gcaaaatcaa acactcatgc aatactttga atggtatctg
     2701 ccccacgacg gtcaacactg gacgcgtctg gctgaaaatg ctccacacct agctcatctg
     2761 gggatcagtc acgtctggat gccaccagcc ttcaaggcaa ccaacgaaaa agatgtcggc
     2821 tatggggtct atgacttatt tgacttagga gagttcaacc aaaaagggac tgtccgcacc
     2881 aagtatggtt tcaaagaaga ctatcttcaa gccattcaag cccttaaagc acagggaatt
     2941 caacctatgg ccgatgtagt tctcaaccac aaggctgctg ccgatcacag ggaagccttt
     3001 caggtcatcg aagttgatcc tgtagaccgt acagttgaac ttggagaacc cttcaccatc
     3061 aatggctgga ctagttttac cttcgatggt cgccaagata cctataatgg cttccactgg
     3121 cattggtacc acttcactgg tacagactac gatgccaaac gcagtaaatc tgggatttat
     3181 ctgatccaag gggacaacaa gggctgggct aacgaggaat tggtcgataa cgaaaacgga
     3241 aactacgact acctcatgta tgccgaccta gactttaaac atcctgaagt catccaaaac
     3301 atctatgact gggctgattg gttcatggaa acgactggtg tagctggttt ccgtttggat
     3361 gccgttaagc atattgactc tttctttatg cgcaacttca tccgcgatat gaaggaaaaa
     3421 tacggtgacg atttctatgt ttttggtgaa ttttggaacc cagacaagga agccaatctg
     3481 gactatctcg aaaaaacgga agaacacttt gaccttgtcg atgttcgtct ccaccagaat
     3541 ctctttgaag ccagtcaagc tggcgcaaac tatgaccttc gtggcatttt cacagatagc
     3601 ctggttgaac tcaagcctga caaggctgtg acttttgtcg acaaccacga tacccaacga
     3661 ggacaagccc ttgagtctac cgttgaagaa tggttcaagc cagcagccta tgccctcatt
     3721 ttgttacgcc aagacggcct tccatgtgtc ttttacggag actactatgg gatttcaggc
     3781 cagtatgctc aagaagattt caaagaaatc cttgaccgcc tcctagccat ccgaaaagat
     3841 ttggcctatg gagaacaaaa tgactacttt gaccatgcta actgtatcgg ttgggtacgt
     3901 tcaggtgctg aaaatcaatc cccaatcgca gtccttatct caaatgacca agaaaacagc
     3961 aagtcaatgt ttgtcggtca agaatggact aatcaaacct ttgtagattt acttggtaac
     4021 caccaaggtc aagttacaat tgatgaggaa ggttatggac aattccctgt ctcagctaga
     4081 tccgtaagtg tctgggcagt caataccatc taatagctca taataaccaa gctaggtcca
     4141 agcggatttg gcttttttgt attcacaaaa agacctaccc aaatggatag atctttactt
     4201 gattacaatt tacctgctac tgcatccaac aattcttgga tcttagcttg gttgcttcct
     4261 cctgccatgg ccatgtctgg tttaccacca ccacgtccat cgatgattgg tgctaattct
     4321 ttgacaaggt ttcctgcatg aaggtctttt gtcttgcttg ctacaaggac attgactttg
     4381 tcaccgatag cggcaactag gacaagaaga tcagagtagt ctttttgttt ccagttatct
     4441 gcaaaggtac gaagggcacc tgcatcggat acagacactt gactagcaat gtaacgatga
     4501 ccgttgactt ccttaacatc tttgaagata tcgcctgcgg ctgcagctgc ggctttttct
     4561 ttcaactcag cattttcttt ttgaagttga cgaagttgtt cttgaagtcc ttctaccttg
     4621 tgaggtactt ccttgacttg aggtgctttc aaggttgctg cgatagcttt aagagcatcc
     4681 tcttgttcac gataggcttc aaaggcttcc ttaccagtca ctgccaagat acggcgagtt
     4741 cctgaaccga ttccttcttc tttgacaatt ttgaagagac caatctcaga agtgttgcca
     4801 acatgagtac caccacaaag ttcaatagag tagtcaccga tagtcacgac acgaacttcc
     4861 ttgccgtatt tctcaccaaa gagggccata gctcccattt ctttagcagt gtcaatatcc
     4921 gtttcaactg tcttaacttc aagagcttcc cagatttttt cgttgacttg ctgttcaatc
     4981 gcacgcaatt cttcagcagt tacagcttgg aagtgggtaa agtcaaagcg aaggaattca
     5041 acttcgttaa gagatcctgc ctgtgttgcg tggtttccaa ggatattgtg aagggcagcg
     5101 tgaagcaaat gagtcgcagt gtggtttttc atgacacggt gacggcgatt gctatcaatt
     5161 gccaaggtat attcttggtt caaggcaagc ggtgcaagga cttcaactgt atgaagggct
     5221 tgaccatttg gggctttctg aacattggtc acagtagcca caaccttacc tgactcatcc
     5281 aagatttgtc cgtggtcagc gacctgtcca cccatttcag catagaatgg cgtttccgca
     5341 aagataagtg aggtagttcc ttctgaaaca gcttctactt ctgcattgtc cgccacgata
     5401 gctaccaatt tagaagacaa ttggctagca ttgtagttga agacactttc tacagtgatg
     5461 ttttgaagag tttcattttg catacccatt gagccaccct tgacagctga ggcacgcgcg
     5521 cgttcttgct gttctttcat ggctgcttca aaaccttcac ggtctacagt cataccagct
     5581 tcttcagcga tttcttcagt caattcaact gggaacccat aagtatcata gagtttgaag
     5641 acatctgaac cagcgataac agattgacct ttttctttca agtctgctac aatgccttgg
     5701 gcaaagtgtt gacctgagtg aagggtacgg gcaaatgatt cttcttcgct cttaacgatt
     5761 ttctcaataa agtcacgttt ctcaagcact tctgggtagt agctttccat gatttttcca
     5821 acagttggaa ccaatttgta aaggaaaggt tcgttgatac ccaatttttg accatgcata
     5881 gaagcacgac ggagaagacg acgaagaaca taaccacgac cttcatttcc tggaagggca
     5941 ccatcaccga tagcaaatga aagagaacga atgtggtctg cgataacctt gaagctcatg
     6001 ttgtcgccat cttggtcata aaccttacca gacaatttct cgacttcacg gataatcggc
     6061 atgaagaggt ccgtttcaaa gttggtctta gccccttggg taacggccac caaacgctcc
     6121 aaaccagcgc ccgtatcaat gttcttatgt ggcaattcct tgtattcgct acgaggaaca
     6181 gcagggtctg cgttaaattg tgacaaaacg atgttccaga tttcaatata acggtcgttt
     6241 tcaatatctt ctgcaagcag gcgaagaccg atattttctg ggtcaaaggc ttccccacgg
     6301 tcaaagaaga tttctgtatc tggtccagaa ggtcccgcac cgatttccca gaagttgtcc
     6361 tcaattggaa tcaagtgact tggatccact cccacttcaa tccagcggtt gtaagaatct
     6421 ttatcgtctg gatagtaggt catgtaaagt ttttcagcag ggaaatcaaa ccattcaggg
     6481 cttgtcaaaa gctcataagc ccaagtgata gcttcgtcac ggaagtaatc cccgatagag
     6541 aagttcccca acatttcaaa catggtatgg tgacgcgcag tcttccctac gttttcgatg
     6601 tcgttggtac ggatagcctt ttgggcattg gtaatacgtg gattctcagg gataatggtc
     6661 ccgtcaaagt atttcttgag ggttgctacc ccagagttga tccacaaaag agttgggtcg
     6721 tttacaggaa ccaaacttac tgatggttct actgagtgac ctttggtcgc ccagaaatca
     6781 agccacattt ggcgtacttg tgcactagat agttgtttca tattgtctcc ttatttactt
     6841 gtttaatgtg attggctttc cagcatttcc acatagtcaa tcgcgacaca gagggaaatg
     6901 actaggtctg cataagcgtc ctcaagaacc gttacggtat aggtagaggt cagatggaag
     6961 agttccttct taatttccgc aatcagctga tcgcgatcat ccagcaattt gaaattcaaa
     7021 tcccagatat tgccctcgat acgaagacct agattatcaa actcatactt atctcgccag
     7081 aaggtcaact tcttacgaat gacaaaactc gagccatccc gaagctgaat ctcaaaacga
     7141 ggaagcaagg tcaagatttc tttactaatc tcactgactt gttcaccagc cgcatcatag
     7201 atggtaaagg ttttaggaat cttaaaaaat gatccctcca cctgataggc aatttctccc
     7261 ctgtcatcct tgatagcgaa gcgttcgcct ccaagacgaa acttttgttt gacaagaaat
     7321 gttttcatca acacctccaa aaatcaaaag acaagctcat atcacgaagg gcgaaaaacc
     7381 gcggtaccac cttcattcaa tgaacttgtc attctcttgt tcttatgcaa ttgtatgatt
     7441 gagtagcatg acttcctagc ttagatggct cgcagcaccg ccatttctct ggactaagac
     7501 aagtgaaaat caattctcaa ctttcttatt ataacgtttt tttaagcttg cgtcaactgg
     7561 aaatgatctc cgttgaatta gaccaattcc ctacatctct gattactttt tcaggatata
     7621 ttttttctta ctgccgtttt tctttttatc ccaaattttc atattactaa acacagctac
     7681 tagaatattt ccaaatataa aggtgcctat cacccaatat atggactcag ttgttaggta
     7741 ttgtcgatcc aagccatcct ttaaatggga tagtataaca gtttggttaa caatcataaa
     7801 ggttggccag aaactttttt tgaaaaaagt agacattttc attatttgtt gccgctttct
     7861 gtaaggttaa tactcaataa aaatcaaaaa gcaaactagg aagctagcct caagctgtac
     7921 ttgagtacgg caaggcaacg ctgacgtggt ttgaagaata taggcttagt atactactag
     7981 gcaagcaaat aagcaactag aatagaaaaa gatagggttc taaaaactga cttctattcc
     8041 ttaaaaacga accagcttga ctgattcgtc ttcttacgtt tatctcctac ttccgataca
     8101 ttttaaactg taggaagagg tcgctatatt tccctgtcca tttatggtca aatttctcat
     8161 aaacttctag gtgtttcatg gtttcaacat ctggatagaa ggccttatct tcctttgttt
     8221 cctctgggag caattccttc gctggtaggt ttggtgttga atagccgaca tactccgcat
     8281 tttggagagc gttttcaggt ttcaacataa agttgataaa ggcataggct gagtcttggt
     8341 ttttaactgt tttgggaatg accatattgt caaaccaaag attgctggcc tctgtcggta
     8401 ccacataacg tagattttca tttttttcta acatttggct ggcttcacca gagaaggtca
     8461 cgccgattgc aacattattc tgaatcatgt agcccttcat ctcgtccgca acgatagcct
     8521 tgatatttgg agtcagtttg tagagcttat ccactgtctc ttccaactgc tgcggatcct
     8581 tggagttgag gctgtagccg agggaattga gtcctagtcc cagcacctca cgcgccccat
     8641 caaaaagcat gatagaattc ttatactcca gcttccaaag gtcatcccaa tgctcaggcg
     8701 cttcatctac catggtttcg ttgtagacaa ttcctaaggt tccccagaag taagggatgg
     8761 agaatttatt acctgggtca aaggactggt tgagaaactc tggtccgata ttttcgattc
     8821 cttcaatttt tgaataatca agcggaacca agaggtcttc gtccttcatc ttgttaatca
     8881 tgtattcact tggaatggca atatcgtagg tcgttccacc ctgctttatc ttagtgtaca
     8941 tggcttcgtt ggagtcaaaa gtctcgtact gaacttgaat tcctgtttct tctgtaaact
     9001 gagtcaagag ttcaggatcg atatagtctc cccagttata gataaccaat ttttgactat
     9061 ctcgactatt gattttacta tctaaatgag tcgcaattcc ccacaagaca aggataatcg
     9121 ctgcaattcc tgctaaaaat gaatagattt ttttcatgct tgctcctcct tctcacgaga
     9181 gataaagtaa taacctacaa ctaggataat actaaagaga aagactagag cagacagggc
     9241 attgatttct aaggaaatcc ccttgcgagc acgagagtaa atctcgactg atagggttga
     9301 aaagccattt cctgttacaa agaaggtcac ggcaaagtca tctaacgaat aggtgaaggc
     9361 catgaaataa ccagtaatga tagacggagt caggtaagga agcatgattt ccttgaacat
     9421 ctgaaattga ctagctccca agtcataggc cgcatgaatc atgtcgccat tcatttcctt
     9481 gagtcgaggc aagaccatca agaccacgat aggaatggag aaggccacgt gactagatag
     9541 aacggtcaaa aagccaagtg aaaacttgag ttgggtaaag agaatcaaga agctagcacc
     9601 aatcataacg tcaggcgcaa ccatgaggat attattgagt gatagaaagg cttcttggta
     9661 tttcttacga gactggtaga tgtaaatggc accaaaagtc ccgataatgg tcgctatcaa
     9721 ggctgatagg aaggccaaga aaaatgtctg agccaaaatc agcatgagtc tcccatctcc
     9781 aaacatggtt tcaaagtgag tccagctaaa acctgtaaag ctattcatat catcaccagc
     9841 attaaaggca tagccaatca agtaaaagat aggcaggtag aggaccagaa agaccagtcc
     9901 cagataaagg ttggcaaatt ttttcatcgt tctctccttt ccttagtcac ccacatggtg
     9961 atgaacatgg tcaggatgag aatcacaccg atggttgaac ccataccata gttgtcattg
    10021 gttagaaaat tctgctcaat agccgtcccc aaggtgataa cgcggttccc accaatcaaa
    10081 cgggtcagca tgaagagact caaacttggg ataaagaccg actgaacccc acttcgcaca
    10141 ccgttcatag atagagggaa gatgacatga cggaaggtct cccacttagt tgcaccaagg
    10201 tcataactag cattgatgag attattatcc atgtcgtcta agacattgaa aatcggcaaa
    10261 atcataaagg ggagctcgat gtagcttgcg acaaagataa aggaaaaatc ggtaaaaagc
    10321 aactgttgtg aaccaattcc gataaattcc aagaattggt taatagagcc attttgacca
    10381 aaaatcccga taaaagcata ggctttaagg agcaaattga tccaggtagg aaggataatc
    10441 agcatgagcc agagttgacg gtgcttgaga cgggtcaaaa agagggctgt tggatagctg
    10501 ataagcaagg tcacaaaggt cacaatccct gcataaagca ctgagttgaa actcattttg
    10561 aggtaggtca agttttgtga cgcaaagtaa gatttataat tttctaaact aaactggcct
    10621 tcaatgttga aaaaggattg tccgaaaatc aagaccaagg gtgccaatac aaagagggca
    10681 atccaaagca tgtagggcac tacaaagagt ttagagcttg ttttcttcat ctctttcctc
    10741 ctcgatcgca ttgatcagac ctgcttcttg ctcttcgatt tccacgtatt cttcgatacg
    10801 agcatcgaac tcttcttcgg tttcattgag acgcatgatg tggatgtctt ctgattcaaa
    10861 gtccagaccg atttcctcac ccacgatggc cttacgggtt gagtggatca tccattcatt
    10921 tccaagttcg tcataggcga taatttcata atgaactcca cggaagagct gggtgtcgac
    10981 cttaacttgg agtttgcctt cttcaggaag ggtaatgcgc aagtcctctg gacgaataac
    11041 gacctcaaca ggttcatttg gcttcatccc accatcaacc gcttcaaagc gtttgccgtt
    11101 aaattcgacc aagtagtcct caatcatggt acctggcaag atgtttgact ccccgataaa
    11161 ggtggcaaca aagtggttga ttggctcatc gtagatgtcc acaggggttc cagactggac
    11221 aatctcgcca tcattcataa cgaaaatcca gtcactcatg gcaagagctt cttcctgatc
    11281 gtgagtgaca aagacaaagg taatgcccaa tcgttgttgt aattcacgca attcgtactg
    11341 catgtctgtt ctcaatttca agtccagcgc tgataaaggc tcgtccaaca agaccacacg
    11401 gggttggttg atgatagcac gggcgatggc cacacgctga cgttgtcctc cagaaagttt
    11461 gcggatggaa cgtttttcat aaccttccaa ctgaaccatc ttgagaactt ccgctacacg
    11521 ctgctcgatt tctttcttat caattttacg caagcgaagt ggaaaggcaa cattttcaaa
    11581 cacattcata tgtgggaaca aggcatagga ttggaagacg gtatgtacgt cgcgcttgtt
    11641 ggttggaata tcattgatac gaacaccgtc tagcatgata tctcctgtcg tcgcatccag
    11701 taaacctgca ataatgttta ggatagttga tttccccgaa ccagatgcac ctagaagggt
    11761 gtagaatttc ccttcttcca actcaaagtt gatgtctttg agaaccttgg tgttgctgtc
    11821 ttcaaaaact ttagagacgt ttttgaattc gataattggt tttttcaatt ggcataaatt
    11881 ccttcttttt catagattaa ccgatcgggg ctctgtcagg tccccactac ctcttgcagg
    11941 gagtaaaacc acctgcatac atcttcgcta ccgataggct ttcacccaag atccggactt
    12001 ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat tggatcaagt
    12061 cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca tgcttttctg
    12121 acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt aactgacctg
    12181 caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc aaaggttgct
    12241 tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga tgggttcctg
    12301 gagctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata gctgaatggc
    12361 ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc ttggtcaaga
    12421 ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca ttcataaaga
    12481 cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtt aaactatgac
    12541 ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca atggtatagc
    12601 catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga atcccaccat
    12661 cacgaacgat gatattgctt gcattgccaa gaaccatcca agggatattt tcttggttgg
    12721 caaatttcac aacgcgagcc aactcaaaac gatttcgtgg aaagaccaaa taatcagcct
    12781 ctccacctac ttttgtataa ctatagctat gcaagggttc cttaaaacgg atatcaattc
    12841 cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt ttacaaaatt
    12901 cattccatta taccattttt agagacattt gacgaccata aaaatacctt gtttggattt
    12961 tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt tccttggttc
    13021 cataggcgat cagaacgagc tccagtgcta gaatcacttc aaccaagact ggatttgtca
    13081 accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag ccataggctg
    13141 ttaggagata aatccatttc ttctggcgaa cagcttgata aacccaaact gtcaaaagta
    13201 attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc cagaccgaaa
    13261 gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt ggattctgag
    13321 tttgaactgc cgaaagaaca atgtaaagat tgagtaaact agtaaggcct agaaaaatca
    13381 actccaagcc accatgcccc agtccatagg ccaaggcgtc tgccttttcc aaacttatct
    13441 ttttctccaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat gctgccatgg
    13501 ctaggccata gataatatag acaagcgggt gatcttgcaa gagggcaata ctaccgtctt
    13561 tttgaggatg caaaatcaag atatgcacca gtttttccaa aatctgcgaa gagacaaaga
    13621 aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt ttggcaaacc
    13681 aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata tgaatggtca
    13741 ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca ttgaacttag
    13801 caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca actgctccca
    13861 cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag gtcaaatcat
    13921 ctgatatgaa gtgcagatgg tagcctgcca cactgacccc atggaaaatc tcaggcgtcc
    13981 agaaaccaac aatagtcccc acaacattgt cacgactata ttccggttga tgggttgcga
    14041 catcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc atatgtgaaa
    14101 attctccccg aatcttgata gagcggaaaa gattttcccc atcataataa gactcaattc
    14161 gttcttccaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc tctgcctgat
    14221 gcggtactac tgctgcataa ggaataaggg catctggtga cacttccaca atttctggct
    14281 gttctcctgt tccttttgcc tgataagcct tgccatctaa gacaatcaat tccccatcaa
    14341 tagaatccaa ggttcccaaa ccaaggtcac catgctctag caattctccc actgtcatgg
    14401 taccaccata aaggccagcc atcaaggctc caagagtatt gtattgaaat aatttcactg
    14461 gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat cttactccta
    14521 agtataccac atttgcccct agatgtgaac gagagaaacg ctctagacat tgccaagaag
    14581 gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga gacaaagcaa
    14641 gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat ttccgacggg
    14701 tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca aggacaccat
    14761 tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc ctcagagtgg
    14821 ctactatgta ttagaacaag ggcaacatca agacctagaa atcgaggtta ccgacgaaca
    14881 tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg gccgagaaaa
    14941 ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac agtccattca
    15001 caaactcctc tttgagcaag ctctctactg caaggctaac caactagtac tgacttctgg
    15061 aacccaacaa gccttgttta tcctctctca aatatccttt cctagacaag ccaaggaaat
    15121 cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac aggggctgga
    15181 ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg aaggccactt
    15241 caaaacagga aaaattaaat ttttctacac cattccccga tttcactatc ctctgggaca
    15301 ttcctattct gagcaagaca aacgatctat tcttaactta gctgccaagt atgatgtcta
    15361 tatcgtagag gacgattatc tgggtgattt ggactccaag aagggccaaa ccttccacta
    15421 tcttgataca gaggagcgtg tcatttatat caagtccttc tcgaccagcc tttttcctgc
    15481 ccttcgtatt acagcactca ttcttccaaa tgctatcaaa gaagcattta tggcctacaa
    15541 aaatatccta gactacgaca gcaacctcat tatgcaaaag gccctgtcac tctatattga
    15601 cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat cttaccaaaa
    15661 acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatc ccctacacga
    15721 tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac acagtcaact
    15781 gggcttggac ttctttgaag aggcctattt aagcacctgt ccttatcaat ttgccaaggt
    15841 gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg aatgacatcc
    15901 aactctgctt tttcttattc ttcaacttca gttagacttg ctgctttttc aagatagctt
    15961 tggtaagttc cttcatcctt aagtttttgg atgaccttgt ctaccacttc tttcaaatca
    16021 tcactatttt ttctaagtgc aacggcattg gcgtcgccgt ccttcatctt caagctgaca
    16081 gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacagg ctcatccata
    16141 tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat attagttagg
    16201 gaagttaatt gagcttttgg caattgttcc ttgaccattg attctggaac agtccctttt
    16261 tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc cacatcagcc
    16321 ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc aaagacttct
    16381 tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc agtttgaaga
    16441 ctggtcaaaa cattgtcaaa actcatgctt aagatttcca acttaacccc aagttcatca
    16501 gcgatagctt gagccatgtc gatgtctgca ccgactacct ggttctttcc atcaaccaat
    16561 gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt ccccttttgc
    16621 ttaatggcct caacagctga ctgagaacca ttagaacctg actggcaagc tactaagaag
    16681 aaacttgcaa gaaaattaca taaaacaaat atccattttt ttgatttcat aaataaacaa
    16741 cctttctgtt aattttgtat aattataaca atatccaagt tacttgtcaa gtgtttttta
    16801 aatttttatc tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt gatttcaagc
    16861 tcctttttat acagaattaa actattttat agttcgacaa tcttacctgt ttcaaagtag
    16921 acaacccatt cacagatatt tttagcatag tcaccgatac gctccaagta ggaaataact
    16981 tggaaataat cacgacccgt aacaatggct tctggatttt tcttaatctc ttcagtcgca
    17041 aggtcacgga tagtttcaaa atagtggtta atttgctcat ccatggaggc cacccggtat
    17101 gcgtcgtcaa cagaaccatt aagataaaga tcaagtgctg cttcaacaac gcttttaact
    17161 tcacgtccca tttttttaat ttcttcctct acagctggga tgcgttcttc ccccttcata
    17221 cggatggttg cctgggcaat ggctacagcg tgatccccca tacgctccac atctgataca
    17281 gccttaagga cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc
    17341 atttcaaatg atttcttttc cagtttcact tcgtattcat ttacttctgc atcatcttct
    17401 atgacctctt ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt acgattgatt
    17461 tgtgagagca cttcttgtcc catagcgtag aactggttat gtaatttctc taaatcttct
    17521 tcaaattgag atcgtaacat ctttcatctc cttatccaaa ttttcctgta atatagtctt
    17581 ccgtttcctt gtgttgggga tcaaggaaca tctgcttggt atcattaaat tcaatcaaat
    17641 ctccatctag gaaaaatcct gtcttatcag agatacgtga agcttgctgc atggaacggg
    17701 ttaccagaag catggtgtac ttgtctttta gaccatacaa ggtttcctca attttaccag
    17761 ctgaaatcgg atccaaagcc gaagttggct catccaagag gatgatttta ggactagttg
    17821 ccaagacacg ggccacgcag acacgctgct gttgaccacc tgacaatcca atagctgaat
    17881 catatagacg atccttgacc tcatcccaga tagaggcacc ttgcaaggct ttttctacgg
    17941 cttcatccag aacctgctta tccttaattc cattgatacg aagcccgtag acaacattct
    18001 catagatagt catagggaaa ggattaggtt gttggaaaac cattccgatt tccttacgta
    18061 attcaaccgt atctgtacgc ggactgtaga tgttgtgacc attgtacacc acggatccag
    18121 ttgtggtcac ctctggattg agatctccca tgcggttgag agacttgagg agggttgact
    18181 tccctgatcc agatggacca atcaaggctg taatttcctt aggttggaaa gatagggaaa
    18241 cactattcaa agccttcttt ttattataat aaacggacag gtctgatacc tgtaaaatcg
    18301 catctgtcat acggtttcct ttctaaccaa agtgaccaga tacatagtca ttggtggact
    18361 gtagcttggc attttggaaa atagttgcag tcttgtcata ctcaatcaaa tcacccaagt
    18421 aaaagaagcc tgtatagtca cttgcacgag cagcctgctg catattatgc gttacaatga
    18481 tgatggtaaa gtttttcttg agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg
    18541 gatccaaggc tgaggctggc tcatccatta agaggatatc tggcttaaca gagatggcac
    18601 gagcgataca gagacgttgt tgctgaccac ctgataaggt caaggctgac ttgtggagat
    18661 cgtctttaac ctgatcccag agggcagcct gacgaaggga ggtttctacg atttcatcta
    18721 ggacttgctt atccttaact ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg
    18781 acttagcaaa tggattgggg cgttgaaaaa ccattccaat gtgtttacgc atttcataaa
    18841 cgttgatttc tggacggttg acatcaattc cacgatagag aatctgccca gttactttag
    18901 caatatcaat agtatcattc atgcgattga gactgcgtaa gtaggtagat ttccccgacc
    18961 ccgacggacc aatcaaagct gtaattttat ttctttcaaa ttgcatatca atccccttaa
    19021 tggattcatt tttaccatag taaacatgga catccttagt agaaagggct actttttctt
    19081 caggaaaggt aaggatatgc ttctcatccc agttatatgt tgacatggct tctcctttag
    19141 gcagcggtta atttcttgtg tagatagctt ccgaacttac gagctccaaa gttaaaaatc
    19201 aggataaaga tcaggagcac agcggcagaa cctgctgata caatggttcc atctggaata
    19261 gtgccttcac tattgacttt ccagatatgg acagccaagg tttctgcttg acggaagata
    19321 gagatggggc tagtcacact gaggatattc cagttagacc agtcaagagc tggcgccgat
    19381 tgccctgctg tatagatcag agctgcagct tcgccaaaga tacgaccaga tgccaagacg
    19441 acacccgtta caatacctgg aagcgcttct ggaataacaa catgaaccac tgtctcccag
    19501 cgagaaatcc caagagccag accagcctca cgttgggtat ggtgaacgtg tttcaaacta
    19561 tcctctacat tacgcgtcat ctgaggcaaa ttaaagactg tcaaggccaa ggcacctgaa
    19621 atgattgaaa atccatactc aaactggact acaaagatca agtaaccaaa gagacccacc
    19681 accactgatg gtaaagagga caagatttca atacaagtcc gcacaaagtt ggtaacagga
    19741 ccttttttag catattcagc caagtagatc ccagctccca tagaaagagg tacagaaata
    19801 atcaaggtaa tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct
    19861 gcttgaaaag cagaagacct tccagtcaag aaagaccaag agatatgggg caagccccga
    19921 accaagatat agagaatcaa ggaagccaag attgtcacaa tgatgctagc aatcgtatag
    19981 aggacagctg ttgcaagttt atctaatttc ttagcgcgca taatttttct ttcctctttc
    20041 tttcgtaatc aatttaatca cactgttaaa aactaaactc atcaagagca gtaccaaggc
    20101 cagtgaccag agaacattat tatttacagt tcccatgaca gtgttcccaa ttcccatagt
    20161 taatatagaa gttaaagttg cagctggtgt ggtcaaggaa gttgggataa cagctgagtt
    20221 tccgacaacc atctggatag ctaaagcctc accaaaggca cgcgccatcc caaagaccac
    20281 tgcagtgaaa ataccagaac gggccgcctt caagatcaca cgccagatag tctgccagcg
    20341 agtggctccc atagcgaaac tggcttcacg ataataacga ggaaccgcac gcaagctatc
    20401 cgttgtcata aaggttacgg tcggcaaaat catgacaaag aggacggaaa tccctgacaa
    20461 aatcccaaaa ccagtcccac caaagacact gcgaacaaag ggaacgacga cttgcaagcc
    20521 aataaatccg tacactactg aaggaatccc aaccaggagt tcaatagctg gttgcaaaat
    20581 cttcgcccct tttggtgata cttcggtcat aaaaactgct gcaccaatag caaagggggt
    20641 tgcgataagg gctgagagaa tggtaacgat aaaggaaccc aaaatcatag gaagggcacc
    20701 aaattcttta ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga tattcacacc
    20761 attgacaaag aaggtcgaca agcctttttg cgctacgaaa accaaaatca tggccacaag
    20821 gatgactatc aaagaaagac aggcaaaggt caaacctttt cctaatttct ccagacgaga
    20881 attctttgat ggaagcaaca ttttcttagc taattcttct tgattcatta ttgtctccct
    20941 tccaacactg tcacagttcc ggcagcatct ttttcaacct tcatttcctt aatcggaata
    21001 tacttcaatc ctttgacaat cccttcttgg gtctcatccg agagaacaaa attgagaaat
    21061 tctgcagcca actcattggg ctgccccaat gtatacatat gctcataaga ccacaagggc
    21121 caattattgc tacttatatt ttctggactt aagtcatagc cattcaactt catgcttttg
    21181 accgaatcat ctatataggt aagagataaa taagagatag ctcctggact ttttgatacg
    21241 attgatttta ccgctccatt tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc
    21301 ataatgacag tatcaaaggt agcacgagaa ccagagccgg ctgcccgatt gataacagag
    21361 atgggtaagt ccttaccacc aacctctttc caattggtta cctcacctat gaagatttga
    21421 cgaagttgct ctgtcgttag gttatcaaca tcaacctcct tattgacaat cagagccaag
    21481 ccagctaccg cgaccttgtg gtcaacaaga gcagaagcat caattccgtc tttttcctca
    21541 gcaaatacat ctgagtttcc tatatcaact gccccagact gaacctggga caagcctgta
    21601 ccagaacctc ccccttggac attgaccgtt tttccaacat ggatcgtgcc aaattcatct
    21661 gccgctactt caaccaaggg ttgcaaggca gttgagccaa cagccgttat ggattctcca
    21721 cgatcaatcc agctagcaca gcctactaaa caagccgtca gccaaaaagc gataagagac
    21781 agagcaagct tttttctttt tttcactgtt tttctcctcg aaaataatta tgaatactgt
    21841 gaatttttta agtagttctt tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa
    21901 ttgattattt atataatata ggctatatta ctctttccta acctcctttt ttcatatgtg
    21961 gataaaatct cttgtctatc ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc
    22021 tttttcccct ttttaatgca agggaaatta ctctccttag atgataatcc aaaagctaga
    22081 aaggtatctc aaacctctct actctcccag actagtttac aactaaaagg aaaagattcg
    22141 attttatgag aaatctagtt tacaagcggt aagaacgcta ataactaaac ttcttgtact
    22201 ctttgaaaat ctcttcaaac cagtgttttg agctatctat ggctagcttc ctagtttgct
    22261 ctttgatttt cattgagtag taaaactaca tgtaatggca atcaagatat caagaatcat
    22321 cctactaaaa aaatccatac tttcactata acatagaata agatatttga ctagcatttt
    22381 catttgaatc tgaggccttt tggaaaataa tttttcaaaa catttccagt aacctttgca
    22441 aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag actttctgcc
    22501 agctgaacga tttctccagc cgcatacttg acaaactctt cttggccaat ttcaaccgac
    22561 tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc aaagcgtttc
    22621 ttcttaaaag tacccagatg cagtccattg cgagcaatct tgagcttccc taaatctggc
    22681 aaaagttctg gcaagagata aagctggtct ccaaaaatct gcaagatacc cggtagattg
    22741 accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg gctgaggtta
    22801 ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgtag atgggcaaca
    22861 aactgaccct ctcccttaaa ctgatgaggg tacatccgag ccgtttctgg caggtcaata
    22921 ccagctacca ttccattgat atgctctact ggcaacaagt caaaatcata ctcttccagc
    22981 aaccaattga caatctcttc gttttcctcg ggtgcccagg tacaggtcga ataaaccaga
    23041 cgaccacctt cagctaacat ggtcactgca tcctccagaa tttctctttg caagctaaca
    23101 cattgactcg gataatctaa gctccaatag tccatagcat caggttgctt acgaaacatt
    23161 ccttcaccag agcaaggggc atcaagaacg attaagtcaa aatagccttt aaagaccttg
    23221 accaagcggt cggcagattc attggtcacc acgacatttg tcgctccaaa acgctccatg
    23281 ttttcaacca aaatcttagc ccgtttgctt gaaatttcat tggaaacaag tagcccctcc
    23341 cctgctagat aggctgccag ttgagttgat ttgccccccg gtgcagcagc caagtccaag
    23401 accttcatac caggactggg ttgggctact tgagccacca tttgagcagc aggttcttgc
    23461 gaataaacta aacctgtagc atgctcaggc gatttccctg aaaccttccc atagtggccc
    23521 caaggggttt gagtaatggc atcagaaaag gaaagttgct cttcttttaa gggattgacc
    23581 cgaaaggccg aaaccgcttc ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct
    23641 agtatttctt tatatttttc aacaaatcct tctggaaatt tcatttaagt tcttttcctt
    23701 tcgtaaatat aggactgaat ttcctcctgc atctcaagag gcaccatcat gaccggctgt
    23761 ctggtttgaa aatcaggagc ttcaccaaaa agggtcacaa cccgatagcc cagactttcc
    23821 cctaaaatac tagctgcggc ataatcccat ggttgcagat aggtgagata ggtcaacaaa
    23881 tgccctgaca aaatcttggc aaaactaatg gccgcacttc catagacacg aacaccaaga
    23941 accgctcggc tcaaatcagc cagcccccat tcattggttt ccagcatacc actattccct
    24001 gcaatgagaa aatctccaag tggtttagtt ttaaaaggag ctaggggcct atcatttaga
    24061 caaactggaa attccccacc accgtggtaa caatcccctt tgaccacatc ataaatcaga
    24121 ccaaactgtc cctgaccatt ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg
    24181 gctacaaaat tattggtacc atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc
    24241 gaggctcgca gacaaccttc ttcagcacaa atcttatcct caggataacg agacaaaatc
    24301 tcaccaacca agagttcctg aacttctttg tccagtctgg tcaccaaatc tgttggagag
    24361 gacttggttt caacacgcaa gtcttcctgc atatggtcaa gaatgtactg gcctgctttc
    24421 ttaacaagcc ctttagcaaa ttcaaattta ctttccaaga gaaatctttc cttccccttt
    24481 ttctttggca gcctgaactg ctcggtaaag tgaatagcca ctaaccgttt caaattctcg
    24541 tcccaaacgt ttctcttcgc tcttgcttgg cacgaccgct ttgaatccct tataggagtc
    24601 cagtaacttt ttagcctcta ctttgccttc ataggcagct tcaacatcat taaaaaaaga
    24661 aagcactgaa gcaagttctt cagtgctcca cgacaaatct agtgggtaac tatactgttt
    24721 gttcattaac taataccagc tctcattctt gcttctttta gttcttgctt acgataacta
    24781 cgagggagaa aagcacgaat ctcatcttca ttaaaaccga tttgcatacg cttggcatca
    24841 ataataattg gacgacgcaa aagactagga tactgctcaa tcaaatgaag caattccgat
    24901 accgaaatac tctctacatc aatattcaat ttttgaaaaa tttttgaacg agttgaaatg
    24961 atgtcatcag taccattttc ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa
    25021 ggactggtca taatattgtg ttccacaaag ggaaccttat gtttttctaa ccaggcctta
    25081 gccttacgac atgatgtaca gctcggtgat agaaatagtg taatcatgct tttctcttct
    25141 tatctatact ttgctacttc tattatacaa aaaaataagg cgcttgacta gggattttta
    25201 gaaaaaaagc ctattttttc aagaaaaata ggctttttgc gaacgattga cacaattgga
    25261 tttggttaat tcactcttaa cgatggtttt aaacgatata tatatttata tatgtaaatt
    25321 aaaaacatct ttcctttcac ttcctacgac ttttcagata cagatagcca aagaagtttt
    25381 catagagggc aaaaaagagg aggaaggcgt gaagaaagaa ggtctctggc aaaatcataa
    25441 taacaggatc cttggctgga tcaaaaagcc aggtatcatc tcccacaaag agaatttgat
    25501 ggaaaagagt aaagaattgg tcaaaaccaa tcaaaactcc cccaagtcca atcatcacag
    25561 gtaagactac tagagccagg agactttttc gataaagaga caaaaagtcc tttttcacaa
    25621 tcctattgac aaagacatag aaacttggca gtgtcactag agctactagc tgaaccaaat
    25681 gaaagagatt cttgaccact gcgaaatggt gcagaccagc tgctgacgaa cgaaaatcag
    25741 gcatctgtaa gacctgacta aaaggattgg tcagataatt catcaagata tgaaaattgt
    25801 attgaacggt ttctggtttt agatagactc gattcgttaa gtttagccac tgaatctcca
    25861 taggatagaa aatccaagcc agataaatgg tcagaaggat tgagagggag aggagaaaga
    25921 gcatagagcc ccaaaaggtc aatttagttt tcatcaaaat cccactccgc aaggctagaa
    25981 accacatgtg tcggtgcgat tggcaggcca gctacttctt ctgccttagt aaaacctgtc
    26041 gtcaccaaga gcgttggaat gccattgtca atcccagccc ggatatcagt caaataattg
    26101 tccccaacca tgattaactc ttcacgttcc aaacctaagt gctcaaccgc cttgtccata
    26161 atgatggcat ttggttttcc gatataaacc ggcttcactc gtgtcgctac ttcaagcagc
    26221 gtaatcagtg agccagtacc tggcaaaaga ccacgttccg tcgggatgtt gaggtcagga
    26281 ttggttccga taaaatgggc acccttttga atagcaagag ttgctgtggc aaatttttca
    26341 tagtcgactt gccaatccag accaactacc acgtaggcag ggttttcctt gtcttccaca
    26401 taaccagccg ccttgatggc ttccttgagt cctgcttctc cgacgacata gacagtcttt
    26461 tcaagcccca aatcattcat atagtcgatg gttgccaaag tcgctgtgta gacagtcgat
    26521 agaggcgtat cgatattaaa attctgagcc aacatctcct taacactctc tggagtgcgg
    26581 gttgtattgt tggttacaaa gagataggga atgtcccgct tttgcaattc atgaacaaaa
    26641 gtctctccag cagggattcg gtctttcccc ttataaatgg ttccgtctaa atcaattaaa
    26701 tagcctttat atttcatcta tttctcccta aacctttttt atttcttgcc aagtaatgat
    26761 tgcttgggca ttgataaccc catcacttgt aatttcatgc ttgctttcca gtccagtccg
    26821 ttcaacagcc gatgtaatca ccccacctgg tcgaacttcc ttgacatact tgaggttgat
    26881 tttcttggga atatagtggg tcaaaaaatc cgctcccatg acctcaaaaa tccagtccaa
    26941 gtatttactg ttattgacat gaccattcat atccaagtcg taaaaacgaa catggtaatc
    27001 cttgctgatc ggctcttcca aggattcata cttcggtcca cggataagtt ttttatcaaa
    27061 atcagactgg taaggagcca caatctcagg ttcaacagca tggacttttc gactgtcgcg
    27121 gtccatgaga acaaaggtcg ccatcatatg gatgagctcc tgccccgctt cattataaat
    27181 agtaaagcga cggtagcaaa aaagtcgatt gtagctcaag gcttccgttt cgatggtaat
    27241 ttcttccgca aaacgaggca aacgaaccac ctcaatatca tattctgcga taatccagac
    27301 cagattatag tcttccaaaa tggccttatc actaactccc agttcaatcg attgcatccc
    27361 tgaaacttgc agtgacagca aaatcacatc tggaagtttg atatgaccgt tcatatcagc
    27421 catatcaaaa ggaattttca ttttcatttg ataagttaag cccatgatcc tactccaaaa
    27481 taaatcgttc tgctacagta tctcccaaaa agagacctct ctttgtcatg cgaacgtggt
    27541 caccctcaat ctgcatgagg ccttgttgaa ccaaatctct gacaatttct ccataaagtc
    27601 cagcaaaaga ctgtccaaat ttttcctcaa atcgcgccat ggaaaccccg gatttcttgc
    27661 ggagtcccaa gaacatttct tcttccattt gctccttttg actcaggtga tcttctgtaa
    27721 tacaagcatt gccttcctca accgcactga gataatgacg aatgggacca tgatttttat
    27781 agcgtactcc attgacataa ccagatgccc ctgcaccaat accatagtat tcagcattgt
    27841 cccagtacat gagattatga cgactttcaa agccgggttt ggagaaatta gaaatctcat
    27901 aatgctcaaa acccgctcgc tccagctctg caatgatgta ctcaaacatc tccgcttcta
    27961 gttcctcctt aggcagaggc aatttcccac gtcgcatccg gttcataaag accgtatggt
    28021 tttctaaaat caaactatac aaactcatgt ggggaatatc caatccaatg gctttagcca
    28081 cattttcctt tacttgctcc atggtctgac caggcagagc ataaatcaaa tcaatggaga
    28141 tgttgtcaaa accagccagt ttcaggcgat cgatattttc ataaatatcc ttctccaaat
    28201 gactgcgccc aatctttttc aacatcttat catcaaaggt ctggacacct agcgaaacac
    28261 gattgacagc cgaatttttc aaaacagcta tcttatccgc atccaaatcg cctggattgg
    28321 cttcaatggt caactcttcc aagacagaca aatccaagtt tttagtcaag ccattcagta
    28381 acacctccag ttgcggagcc gacagggctg tcggtgttcc accaccgata taaagggttg
    28441 acaacttttc aatatcataa gaacgaaact cttccagcag atgctctaaa tagctgtcga
    28501 ctggctgatt tttgatgaag acctttgaaa aatcacaata ataacaaatc tgggtacaaa
    28561 atgggatgtg cacataggct gacgttggtt ttttctgcat agtaattatt ataccacaaa
    28621 gactagattc cagataaaaa tcaccatccc cagatacata gtccgtccgg agatggtgat
    28681 ggtttattct tctgttatat caatcacaat ctcttctgag tcatcaagag cttcggcttt
    28741 ttcttgccat tgttccttga gattatttaa ttgatttttt gatgcttctg tcgcttgaaa
    28801 agcataggat ttagcttgag caagtatact gtccacagtg atttcacctg actcaacctg
    28861 ttcttttgtt ttcagaacaa aatctgtagc ctgctcctta acttctgtca gtttttcaca
    28921 gacttgctcc ttggcatact ccggatcttc tctcaaatca tctagaaaat cttgagcctg
    28981 actgcaaact tgtttgccct tatcacttgt taaaaacaag gcaagagctg cacctgaaac
    29041 ggttcctaaa aggattgagg ataatttacc cataaggatt ctcctttttt attttttgaa
    29101 aaatttactt gcaagacgaa gagctgacag acttgcacca gtcttgagtg tttttgaacc
    29161 agctgatgaa gctttcttgc tcaagacacg cgcatggtca ttgaggtctg aaacagatag
    29221 agataaatct gcaacagcac tgaagagtgg atcaatcgta gccaccttga cattgatatc
    29281 atctgccaag acattgacct tagccaacaa ctcattggtg tgatgcaagg tcacatccac
    29341 atctgaagtc aaggttttaa tcgtcttttc tgtttcatcg atgacacgac caagcttttg
    29401 tacagtaatg atcagataga ccaaaaagac aatcacagct agggcaacaa gaatatatgc
    29461 aacttctaac atttagtttt cctcctctgt aatatagtaa ggggccttct ttcgattttg
    29521 ataaataacg atcattatac cgagaccgat aaagacaact gacagccatt gggacactcg
    29581 aaggccgaag aacatgagac tatctgttcg cataccttcg atgaccatac gaccgaaacc
    29641 ataccaaatc aagtaaaagg ccgtgatatg acctcgtctg agactcttcc atttccgtct
    29701 aaaaatcaga atcaaggcaa agccaagcag attccataga gactcataaa ggaaagtcgg
    29761 ttgacggtag ctcccctcaa tatacatctg gtcacggata aagccaggta gataatccag
    29821 attatccact gttgcaccat aagcttcttg gttaaagaaa ttaccccaac gccccaaact
    29881 ttgagcaatc ataacgctag gcgccgcaat atctagaaaa tcccaagtat tgatgagttt
    29941 acggtcagca aagatataga gcacaagagc cccagttatc aaaccaccgt aaatggccaa
    30001 accaccattc caaatggcaa aaatctctcc taaattctga ctatagtaat caaatcggaa
    30061 aataacatag tagagacgag ctcctaaaat agccaaggga aaggctacta agataaaatc
    30121 taaaatatcg tctggtatga tcttctttct aggtgcttct ttcatggtca aataaaccgc
    30181 aagaatcaag cctgtcacaa tacataaggc ataccaacga atggctaggg gtcctagttg
    30241 aatagcaatt ggatcaagca ttttgcacct catttcgagc tatgagactt gtcagtcgtt
    30301 cgtcgaacaa acgggtagca tcaaagccca tttccttggc acgataattc atggcagctg
    30361 cctcaatcac aacagagata ttacgacctg ttttaactgg aatacgaata cgaggaatgg
    30421 ctacgccaga aacttcaagt tcctctgcat tgtttccaag acgatcaaag gtcttatgcg
    30481 tatcgtaatt ttccaaatag acagcaagct gaacctgtga agaatccttg acagcactcg
    30541 caccgtagag actcataaca tcgataatac caaccccacg aatttcaatc aagtgtttca
    30601 aaatttcagc tggttcaccc cagagagtaa tctcatcctt ggcaaagata tcgacacggt
    30661 catcggctac caaacggtga ccacgtttga caagctcaag acctgtctcg ctcttaccaa
    30721 ttccactatc tccctgaatc aagacgccca tcccataaat atccatcaag acaccgtgca
    30781 cactggtacg ttctgccaaa cgagaatcca gatagctaga taactctcca gataaacgac
    30841 tggtagctgc acggctggtt aaaatagcaa tcttacattc tctagcagcc tttaacatct
    30901 cctctggaac caccaaacca cgggcaacaa tgactgctgg tgtctcaggt agaaacattt
    30961 ttttcaaaac ttcataacgg ctgttggaag gcatgctgat cagataagac cactccttca
    31021 tccccaaaag ttgaatccgc tctggtgtat agtagtcaaa atagcctgtc atttcaagac
    31081 caggtcgcgt aatatccgct gtattgattt ccttttcaag caattctggt tcaccataga
    31141 caatatctag tctaagcttt tcaatcactt cttttactaa aaccgacatc atttcctcct
    31201 tcaaatgaat tcttctctct attatatcaa attgtaggag gaagtttcag atttttgcag
    31261 gctttggagt atttatttaa aagaaaaagg actagatttc tctagccttt catttcaatt
    31321 agaatttttt acgtttacga gctacttctg atttacgttt acgttttaca gaaggttttt
    31381 catagaattc acgtttgcgt gtttcttgaa gagtaccagc tttagtaacc gcacgtttga
    31441 aacggcgaag tgcgtcgtca agagattcat tcttacgtac tactgtttta gacatttttt
    31501 tcactccctt caagttcaat atctatttca ttctacacgt aaaaggaata attgtcaagc
    31561 aaaaatgcgg tttaattttg attttttctt ccatttatac aagagttgaa agcgttcaat
    31621 agaacattgc ttttttattt ttcgagtaag ctaagcgctt cagcatctgc gatgatggtt
    31681 acatcagggt gattttggag gctactcgca ggtaggttct cagtcactgg gccagatact
    31741 gttccggcaa tggcttctgc tttcgactca ccataagcaa aaagaataat agacttggca
    31801 tccaaaatgt ttttaatccc cattgaaata gcttgggttg ggacgtcttc aatcttggca
    31861 aagaagcgag cattggcttc gatagtagac tggtcaagtt ctactagatg cgtttgactg
    31921 tcaaatggag taccaggctc attaaagccg atatgcccat tgcgaccgat tcccaagatt
    31981 tggaggtcaa ctggatggtc agccaaaatt tggttgtagc gttcaacttc agcttcagca
    32041 ttgtccttaa caccacgagg caagaaactt tctttaaatg gtttttggtt gaacaagttt
    32101 tcttgcatga agtaacgata agactgtgga ttgtccccat caaggcccac atactcatca
    32161 aggttgacac tggttagatt tgaaaaatca aggtcactct caacaatttc cttgtaaaat
    32221 tcaagtgggc tacttcctgt cgcaagtcct agagtttgag ctccattagc caatttttcc
    32281 ttcaaaatct caaaagcaac ttttccacct tgaacttggt tttcaacttt aataactttc
    32341 atcttttcat cctccatttc tgtctat
//
LOCUS       DAVHIU010000010        31759 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVHIU010000010 DAVHIU010000000
VERSION     DAVHIU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.010"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(504..596)
                     /locus_tag="VVN06_000386"
     CDS             complement(504..596)
                     /locus_tag="VVN06_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU5561995.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            757..1728
                     /gene="ruvB"
                     /locus_tag="VVN06_000387"
     CDS             757..1728
                     /gene="ruvB"
                     /locus_tag="VVN06_000387"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5561996.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1817..2281
                     /locus_tag="VVN06_000388"
     CDS             1817..2281
                     /locus_tag="VVN06_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5561997.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2512..3270
                     /locus_tag="VVN06_000389"
     CDS             2512..3270
                     /locus_tag="VVN06_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU5561998.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3279..4082
                     /locus_tag="VVN06_000390"
     CDS             3279..4082
                     /locus_tag="VVN06_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5561999.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4104..5363
                     /gene="rseP"
                     /locus_tag="VVN06_000391"
     CDS             4104..5363
                     /gene="rseP"
                     /locus_tag="VVN06_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5562000.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5376..7229
                     /locus_tag="VVN06_000392"
     CDS             5376..7229
                     /locus_tag="VVN06_000392"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5562001.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7329..8708
                     /gene="bglA"
                     /locus_tag="VVN06_000393"
     CDS             7329..8708
                     /gene="bglA"
                     /locus_tag="VVN06_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU5562002.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGICLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            8900..10708
                     /gene="glmS"
                     /locus_tag="VVN06_000394"
     CDS             8900..10708
                     /gene="glmS"
                     /locus_tag="VVN06_000394"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5562003.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10827..11876
                     /locus_tag="VVN06_000395"
     CDS             10827..11876
                     /locus_tag="VVN06_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5562004.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIDGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLDQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDCFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(11976..15773)
                     /gene="spuA"
                     /locus_tag="VVN06_000396"
     CDS             complement(11976..15773)
                     /gene="spuA"
                     /locus_tag="VVN06_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU5562005.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVEIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSIPSK
                     PEHQNEASHPAYQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVQNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16190..16603
                     /gene="rpsL"
                     /locus_tag="VVN06_000397"
     CDS             16190..16603
                     /gene="rpsL"
                     /locus_tag="VVN06_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5562006.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16623..17093
                     /gene="rpsG"
                     /locus_tag="VVN06_000398"
     CDS             16623..17093
                     /gene="rpsG"
                     /locus_tag="VVN06_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5562007.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17518..19599
                     /gene="fusA"
                     /locus_tag="VVN06_000399"
     CDS             17518..19599
                     /gene="fusA"
                     /locus_tag="VVN06_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5562008.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19703..24094
                     /locus_tag="VVN06_000400"
     CDS             19703..24094
                     /locus_tag="VVN06_000400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU5562009.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKFLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24196..24459
                     /locus_tag="VVN06_000401"
     CDS             24196..24459
                     /locus_tag="VVN06_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU5562010.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24452..24730
                     /locus_tag="VVN06_000402"
     CDS             24452..24730
                     /locus_tag="VVN06_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU5562011.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLRAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24751..24903
                     /locus_tag="VVN06_000403"
     CDS             24751..24903
                     /locus_tag="VVN06_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562012.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLPGAIALAGIF
                     SALKEK"
     gene            24925..26166
                     /locus_tag="VVN06_000404"
     CDS             24925..26166
                     /locus_tag="VVN06_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU5562013.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     IFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26176..26406
                     /locus_tag="VVN06_000405"
     CDS             26176..26406
                     /locus_tag="VVN06_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5562014.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26629..27351
                     /locus_tag="VVN06_000406"
     CDS             26629..27351
                     /locus_tag="VVN06_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5562015.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27568..28902
                     /gene="pepC"
                     /locus_tag="VVN06_000407"
     CDS             27568..28902
                     /gene="pepC"
                     /locus_tag="VVN06_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU5562016.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(28958..29869)
                     /locus_tag="VVN06_000408"
     CDS             complement(28958..29869)
                     /locus_tag="VVN06_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5562017.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(29893..30696)
                     /locus_tag="VVN06_000409"
     CDS             complement(29893..30696)
                     /locus_tag="VVN06_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU5562018.1"
                     /translation="MSIISMVLVIVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30724..31713)
                     /gene="manX"
                     /locus_tag="VVN06_000410"
     CDS             complement(30724..31713)
                     /gene="manX"
                     /locus_tag="VVN06_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU5562019.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         9029 a         6128 c         7431 g         9171 t
ORIGIN      
        1 aactccaccc aatcaggtgg agttttttag ctctatttca ggcttttggg gactattcta
       61 aaaataattt ttcgatattt ttcggtattt ttcggatttt ggtcggggaa ttggcgggga
      121 cttttttagc gaatataact aagaaatagg tctgtggtcg cttcagctag ttcaaactca
      181 atttgattgt aagtgactgt taattgaaga actggaacta ctgtaataat gattttgctt
      241 gtctagtagt ggcgtattaa atgagaagtg acatgtaaga ttgtctcttc gtttacgaaa
      301 tcaaaatttc tatgaaattg atagaatccc taacgttctc ttaatcttaa ccgtcttgtc
      361 gtacagcggt tgaagaaaat gtaaaccttt gacaaaattc gtgaactatg ggataataaa
      421 aaggaattga gtactagttc tatatcatag gctagaaaag accccaagct cacgaccaaa
      481 taagcttggg gtcttttttg acactatttg tccttgttta gccatttatc gactatacga
      541 agaacgacac cgaccacaat tggtccgatg atagttttga gtactaattc catcatgggc
      601 tatctcacct cctttcgtag gcggtgtagc agtgtcgtag aatattatac cacataagtg
      661 ctaaacttag gagtcaccca atcaggtggc ttttttgttt gtggcttggt tttttgatat
      721 aatagagcca tgagtagaat tttagataat gagataatgg gggatgagga gttagtagaa
      781 cgcacgctcc gtcctcagta tttacgtgaa tatattgggc aggataaggt taaggaccag
      841 cttcaaatct ttatagaagc tgccaaaatg cgagatgaag cgctggatca tgtcctctta
      901 tttgggcctc caggtttggg gaaaacaacc atggcttttg tcattgccaa tgaactggga
      961 gtcaatctca agcaaacgtc tggtcctgtt atcgaaaaag cgggtgattt ggtagcgatt
     1021 ttgaatgagt tagagcctgg agatgtcctc tttattgacg agattcatcg cttacccatg
     1081 tcagtggaag aagtgcttta tagtgccatg gaggacttct acatcgatat tatgattggg
     1141 gctggtgaag gcagtcgcag tgttcatttg gagttgcctc cttttacctt gattggtgcg
     1201 acgactcggg ctggtatgct ctcaaatcca ctacgggcac gttttgggat tacaggtcat
     1261 atggagtatt atgcccatgc tgacttgaca gaaattgtcg agcggacggc agatattttt
     1321 gagatggaaa tcactcatga ggcagcatct gagttggccc tacgtagtcg tggaacccct
     1381 cgtattgcca atcgtctcct caagcgcgtg cgcgattttg cccagataat ggggaatggg
     1441 gtaatcgatg atattattac cgataaggct ttgactatgc tggatgttga ccatgaaggt
     1501 ttggactatg tggatcaaaa aatccttcgt accatgattg agatgtacag tggaggacct
     1561 gttggtctag gaactctttc tgtcaatatc gccgaagagc gtgagacagt tgaagacatg
     1621 tatgagcctt acttgattca aaaaggtttt atcatgcgga cacggtctgg acgggtggcg
     1681 actgctaagg catatgagca cttaggttat gaatacagtg aaaaataagc aagaaatctt
     1741 agaggctttt agagaaaatc cagatatgat ggccattctg acgatcatcc gagaccttgg
     1801 tttgaaagac tcgtagttgg cagcaggttc tgtcagaaat ttcatctgga atcttttgtc
     1861 agacaaatcc ccttttgatc atgaaacaga tatagatgtg attttctttg atccagattt
     1921 ttcttatgag gaaaccttat tactggagaa aaagctgaga gaggattttc ctcagtacca
     1981 gtgggaattg aaaaatcagg tctatatgca tcagcacagt cctcacactg cttcctatac
     2041 cagttctcgt gatgctatga gtaagtatcc agaacggtgt acggcagttg gactgcgctt
     2101 gaatgaagaa tcagattttg aactctatgc accttatggt ttggaggata ttttgaattt
     2161 tcaagttcgt ccaactcctc atttcttaga aaatgaagac cgaatggaac tctatcaaac
     2221 acgtttatcc aagaaaaatt ggcaggagaa atggaaaaat ttgattttta aaaatactta
     2281 aggaaacttt aagctaggga gtgtacacta agttcataag ttaagaagac cttaacttaa
     2341 actcctaaaa ctttttcata ataatctccc tataaaaata aagtcgccca atcaggcggc
     2401 ttattttttt gaaaaatggg catggtgcct gagaataaat agcttagtga tagaagaaaa
     2461 tggggaaata tggtataatg aaacgataga tttttgaata ggaataagat catgtttgga
     2521 ttttttaaga aagataaggc tgtggaagta gaggttccga cacaggttcc tgctcatatc
     2581 ggcatcatca tggatggcaa tggccgttgg gctaaaaaac gtatgcaacc gcgagttttt
     2641 ggacataagg cgggcatgga agcattgcaa accgtgacca aggcagccaa caaactgggc
     2701 gtcaaggtta ttacggtcta tgctttttct acggaaaact ggacccgtcc agatcaggaa
     2761 gtcaagttta tcatgaactt gccagtagag ttttatgata attatgtccc ggaactacat
     2821 gcgaataatg ttaagattca aatgattggg gagacagacc gcctgcctaa gcaaaccttt
     2881 gaagctttaa ccaaggctga ggaattgact aagaacaaca caggattgat tcttaatttt
     2941 gctcttaact atggtggacg tgctgagatt acacaggcgc ttaagttgat ttcccaggat
     3001 gttttagatg ccaaaatcaa cccaggtgac atcacagagg aattgattgg taactatctc
     3061 tttactcagc atttgcctaa ggacttacga gacccagact tgattatccg tactagtgga
     3121 gaattacgtt tgagcaattt ccttccatgg cagggagcct atagtgagct ttattttacg
     3181 gacaccttat ggcctgattt tgacgaagcg gccttgcagg aagctattct tgcctataat
     3241 cgtcgtcatc gccgatttgg aggagtttag gaggaaatat gacccaggat ttacagaaaa
     3301 gaaccttgtt tgcagggatt gccctggcta ttttcctacc aattttaatg attgggggcc
     3361 tcttgcttca gatagcaatc ggaatcatag ccatgctagc catgcatgaa cttttgaaga
     3421 tgagaggtct agagaccatg acgatggagg gcctcttgac cctctttgca acctttgcat
     3481 tgaccattcc cttggagaat tacctgactt ttttgccagt tgatgggaat gtggttgcct
     3541 atagtgtttt gatttcaatc atgttaggaa cgaccgtttt tagcaagtct tatacgattg
     3601 aggatgcggt tttccctctt gctatgagct tctacgtggg ctttggattt aatgctttac
     3661 tagatgctcg tgttgcaggt ttggacaagg ctctcttagc cttgtgtatc gtctgggcga
     3721 cagacagtgg tgcctatctt gttgggatga actatgggaa acgaaagtta gcaccaaggg
     3781 tatcgcctaa taaaaccctt gagggtgcct tgggtggtat tttaggagca attttagtaa
     3841 ccattatctt tatgatagtt gacagtacag ttgctcttcc atatggaatt tacaagatgt
     3901 cagtctttgc tattttcttt agcattgctg gacaatttgg tgatttacta gaaagttcga
     3961 tcaaacgtca ttttggtgtt aaggattctg ggaaatttat ccctggacat ggtggtgttt
     4021 tggatcgttt cgatagtatg ttgcttgtat ttccaatcat gcacttattt ggactctttt
     4081 aatcaaaaga cggaggaaac gctatgctcg gaattttaac ctttattctg gttttcggga
     4141 ttattgtagt ggtgcacgag ttcgggcact tctactttgc caagaaatca gggattttag
     4201 tacgtgaatt tgccatcggt atgggaccta aaatttttgc tcacattggt aaggatggaa
     4261 cggcctatac cattcgaatt ttgcctctgg gtggctatgt ccgcatggcc ggttggggtg
     4321 atgatacaac tgaaatcaag acaggaacgc ctgttagttt gacacttgct gatgatggta
     4381 aggttaaacg catcaatctc tcaggtaaaa aattggatca aacagccctc cctatgcagg
     4441 tgacccagtt tgattttgaa gacaagctct ttatcaaagg attggttctg gaagaagaaa
     4501 aaacatttgc agtggatcac gatgcaacgg ttgtggaagc agatggtact gaggttcgga
     4561 ttgcaccttt agatgttcaa tatcaaaatg cgactatctg gggcaaactg attaccaatt
     4621 ttgcaggtcc tatgaacaat tttatcttag gtgtcgttgt tttttgggtt ttaatcttta
     4681 tgcagggtgg tgtcagagat gttgatacca atcagttcca tatcatgccc caaggtgcct
     4741 tggccaaggt aggagtacca gaaacggcac aaattaccaa gattggctca catgaggtta
     4801 gcaactggga aagcttgatc caagctgtgg aaacagaaac caaagataag acggcaccga
     4861 ctttggatgt gactatttct gaaaagggga gtgacaaaca agtcactgtt acacccgaag
     4921 atagtcaagg tcgttacctt ctaggtgttc aaccgggggt taagtcagat tttctatcca
     4981 tgtttgtagg tggttttaca actgctgctg actcagctct ccgaattctc tcagctctga
     5041 aaaatctgat tttccaaccg gatttgaaca agttgggtgg acctgttgct atctttaagg
     5101 caagtagtga tgctgctaaa aatggaattg aaaatatctt gtacttcttg gcaatgattt
     5161 ccatcaatat tgggattttt aatcttattc cgattccagc cttggatggt ggtaagattg
     5221 tgctcaatat cctagaagcc atccgccgca aaccattgaa acaagaaatt gaaacctatg
     5281 tcaccttggc cggagtggtc atcatggttg tcttgatgat tgctgtgact tggaatgaca
     5341 ttatgcgact cttttttaga taatcgagga atattatgaa acaaagtaaa atgcctatcc
     5401 caacgcttcg cgaaatgcca agcgatgctc aagttatcag ccatgctctt atgttgcgtg
     5461 ctggttatgt tcgccaagtt tcagcaggtg tttattctta tctaccactt gccaaccgtg
     5521 tgattgaaaa agctaaaaac atcatgcgcc aagaattcga aaagattggt gctgttgaga
     5581 tgttggctcc agcccttctt agtgcagaat tgtggcgtga atcaggtcgt tacgaaacct
     5641 atggtgaaga cctttacaaa ctgaaaaacc gtgaaaaatc agactttatc ttaggtccaa
     5701 ctcacgaaga aacctttaca gctattgtcc gtgattctgt taaatcttac aagcaattgc
     5761 cactcaacct ttatcaaatt cagcccaagt atcgtgatga aaaacgccca cgtaatggac
     5821 ttcttcgtac acgtgagttt atcatgaagg atgcttatag tttccacgct aactatgata
     5881 gtttggatag tgtttatgat gagtacaaag cagcctatga gcgtattttc actcgtagtg
     5941 gtttagactt caaggctatt attggtgacg gtggagccat gggtggtaag gatagccaag
     6001 aatttatggc cattacatct gctcgtacag accttgaccg ctgggttgtc ttggacaagt
     6061 cagttgcctc atttgacgaa attcctgcag aagtgcaaga agaaatcaag gcagaattgc
     6121 tcaaatggat agtctctggt gaagatacca ttgcttactc aagtgagtct agctatgcag
     6181 ctaacttaga aatggcaaca aacgagtaca aaccaagcaa ccgtgttgtc gctgaagaag
     6241 aagttactcg tgttgcaacg ccagatgtta aatcaattga tgaagttgca gctttcctca
     6301 atgttccaga agaacaaacg attaaaaccc tcttctacat tgcagatggt gagcttgttg
     6361 cagcccttct agttggaaat gaccaactca acgaagtcaa gttgaaaaac cacttgggag
     6421 cagatttctt tgacgttgct agcgaagaag aagtggcgaa tgttgttcaa gcaggatttg
     6481 gttcacttgg accagttggt ttgccagaga atattaaaat tattgcagat cgtaaggtgc
     6541 aagatgttcg caatgcagtt gtcggtgcta acgaagatgg ctaccacttg actggtgtga
     6601 acccaggccg tgattttact gcagaatatg tggatatccg tgaagttcgt gagggtgaaa
     6661 tttccccaga tggacaaggt gtccttaact ttgcgcgtgg tattgagatc ggtcatattt
     6721 tcaaactcgg aactcgctac tcagcaagca tgggagcaga tgtcttggat gaaaatggtc
     6781 gtgctgtgcc aatcatcatg ggatgttacg gtatcggtgt cagccgtctt ctttcagcag
     6841 tgatggagca acacgctcgc ctctttgtta acaaaacgcc aaaaggtgaa taccgttacg
     6901 cttggggaat caatttccct aaagaattgg caccatttga tgtgcatttg attactgtca
     6961 atgtcaagga tgaagaagcg caagccttga cagaaaaact tgaagcaagc ttgatgggag
     7021 ctggttatga agtcttgaca gatgaccgta acgaacgtgt cggtgttaaa tttagcgata
     7081 gtgacttgat tggtcttcca atccgtatca ctgttggtaa aaaagcggcc gatggcattg
     7141 tagaagttaa gatcaaggca actggtgaca ccattgaggt tcatgcagat aacgtgcttg
     7201 aaacgcttga aatcctaagc aagaaataaa aactataatc agaagaaaaa caaggaaaaa
     7261 atgtaactag tttttacctt gttttttctg tataatggga gaaatgagta gataaagagg
     7321 taaatgttat gctaagattt ccaaaggatt ttgtctgggg atcctctact tctggaccgc
     7381 agacagaagg acgtgtagct ggtgacggta agggagacaa tctctgggat tactggttcc
     7441 aagtggagcc aaatcgttac tataatggga ttggttcaga taagacatcg actttttatg
     7501 aaaattggga gcgggatatt gagcttttgt tagagactgg tcacacagtc tttcggactt
     7561 ctattcagtg gtcacggatt tttccacaag gctgtggaaa agtcaaccct caaggtgtgg
     7621 atttttatcg taaggtcttt gaggctatta aggctaaagg aatttgtctg ttagtcaatc
     7681 tctatcattt tgatttacct tttgcccttc aagaggatgg tgatggttgg gaaaataagg
     7741 cgacagtctc agcctatgaa gactatgctc gtttttgttt tgagacttat ggagatttag
     7801 tggatcaatg gattaccttt aacgagccca tcgttcctgt agaatttggt tatttttatg
     7861 atgcccatta tccacataag gtggatgcag aggcagctgt taaggtagcc tatcatacac
     7921 aattggccag cagccgggct gttaaggctt gccatgaact tttgcctgat tccaagattg
     7981 ggattgtcct caacttgaca ccggcttatc cacgtagcca gcatcctgct gatgtcaagg
     8041 cagctcgtat tgcggccctt tttcaggccc aatctttctt agatccatct gtcttgggga
     8101 cttatccaca ggagttggta gaaatcttgc atgaacacgg tcttttacct gatgctacag
     8161 aggaagagtt ggaactcatt cgtgataata cggtggactt ccttggtgtg aactactatc
     8221 aacctttgcg tgttatggct cctcgatttg ctaagcatcc agagagtcca ctcttaccag
     8281 aacattttta cgagccttat gtgatgcctg gacgtaaaat caattctcat cgtggttggg
     8341 agatttatga gcaagggatt tatgacatcg cccaaaatat caaggaaaat tatggcaata
     8401 ttgagtggat gttgactgag aatggtatgg gtgttgaagg ggaagaaaaa ttccgtcaag
     8461 atggaatgat tcaagatgat taccgtattg actttgtaaa aggtcatctt cgtgaacttc
     8521 accgtgccat tgaagatggt gccaactgta agggctactt gatttggacc tttattgact
     8581 gctggtcatg gctcaacagc tataaaaatc gctatggttt ggtcgaatta gacttggaaa
     8641 cgcaagaacg tcgtctgaag aaatcaggcc actggttcaa gaaactaagc gacaataatg
     8701 gattttaaaa aggactctga ctgattatcc caatcggtca gagttttttg cttacaggag
     8761 cttaatttga actataccaa tttttactct tgacaagtga aagaaacaag tatatactgt
     8821 ttttgctgat tctataaaag atgaattaga ctataccaat tttaaggaga aagcacagct
     8881 tgcctgtgtc gtatatacta tgtgtggaat tgttggtgtt gttggaaaca caaatgcaac
     8941 tgatattttg attcaagggc ttgaaaagct tgaataccgt ggctatgatt ctgcgggaat
     9001 ttttgtccta gatggtgctg ataaccattt ggtgaaggcg gttggtcgta ttgcagaatt
     9061 gtctgccaag acagctggtg ttgagggaac aactggtatc ggacatactc gttgggctac
     9121 tcacggaaaa ccaactgagg acaatgctca cccacaccgc tctgagacag aacgttttgt
     9181 cttggtgcat aatggggtga ttgagaacta tcttgaaatc aaggaagaat accttgcagg
     9241 tcaccacttc aaggggcaga cagatactga aatagccgtt catttgattg gaaaatttgc
     9301 ggaagaagaa gggctctcag ttcttgaagc cttcaaaaaa gcccttcaca tcatccgtgg
     9361 ttcttatgcc tttgccttgg ttgactcaca agatcctgaa gtcatctacg tagctaaaaa
     9421 taaatcacca cttttgattg gtcttggaga aggctataac atggtctgct cagacgccat
     9481 ggctatgatt cgtgaaacta accaatacat ggaaatccat gaccaagagt tggtaatcgt
     9541 caaggctgat agtgtggaag ttcaagacta tgatggaaat cgtcgtgagc gtgctagcta
     9601 cactgctgaa cttgacttgt cagatatcgg taagggaact tacccttact acatgctcaa
     9661 ggaaattgac gagcaaccaa ctgttatgcg taaactcatt caagcctaca cggatgatgc
     9721 tggtcaagta gtggttgatc ctgctatcat taaggctgtt caagacgcag accgcatcta
     9781 catccttgca gctggaacat cttaccatgc aggatttgct tctaagaaaa tgttggaaga
     9841 attgacagat acaccagttg aacttggcat ctcatctgag tggggctacg gtatgccact
     9901 tctcagcaag aaaccactct tcatctttat cagccaatct ggtgaaacag cggatagtcg
     9961 tcaagttttg gtcaaggcta atgaaatggg aattccaagc ttaacagtga caaatgttcc
    10021 aggctcaacc ctctcacgtg aagccaacta taccatgctc cttcacgcag gtcctgaaat
    10081 tgccgtggca tcaactaaag cctatacagc gcaaatcgca gcccttgcct tccttgcaaa
    10141 agcagtcgga gaagcaaatg gtaatgctaa agcgcaagcc tttgacctga ttcatgaatt
    10201 gtcaatcgta gctcagtcta tcgaatcaac tctttcagag aaagaaacca ttgaagccaa
    10261 ggttcgtgaa cttcttgaaa caactcgtaa cgccttttac atcggacgtg gtcaagatta
    10321 ctacgtagcc atggaagcaa gtctcaaact caaagagatt tcttatatcc agtgtgaagg
    10381 ctttgctgca ggagaactca agcacggaac cattgccttg attgaagaag gaacgcctgt
    10441 tttggctctc ttgtcagatc cagttcttgc caatcatact cgtggaaata tccaagaggt
    10501 cgcagcccgt ggtgccaaag tcctcactat cgcagaagag aatgtagcca aagataccga
    10561 cgatatcgtc cttacgaccg tacatccata cctctcacca atttcaatgg tcgtaccaac
    10621 gcaattagtc gcttactttg caaccctcca ccgtggcctc gatgtggata aaccacgtaa
    10681 ccttgctaaa tcagtaacgg tagaataagc taaaaaagtc tagttatcta ggctttttct
    10741 aatgagcaaa ttggttgctt gtacttaaga ttcgtgttag aataattttt caaattgaag
    10801 gacagagata gacaaggaga atcacaatgg tagaattggg aatttcaaca tttggggaaa
    10861 taacggagct tgaagggact ggacaaactt acagtcatgc tgaacgcatt cgccagttgg
    10921 tggcagagat tgagctggct gacaaggttg gtttggacgt atatgggatt ggcgagcacc
    10981 atcgggcgga ttttgcagta tcagccccag agattgtcct ggccgctggg gcagtcaata
    11041 ccaagaaaat ccgtttgacc agcgcagtca gcattctgtc aagcatggac ccgattcgtt
    11101 tgttccaaca gtatgccact atcgatgctt tgtcaaatgg acgagcggag attatggctg
    11161 gaaggggctc tttcacggaa tctttccctc tgtttggtta tgacttgaaa gactacgact
    11221 ctctttttga tgagaaatta gacttgcttc agttagtcaa tgaaaagacc aagttagact
    11281 ggcaaggtcg attgactcaa acgattgctg gtaaagaagt ttatcctcgt ccagttcagg
    11341 acaaattgcc cttgtggata gctacaggtg gtcatgtcga atcaacagtg aagattgctc
    11401 aggctggtct accgattgta tatgctatta ttgatggtaa tccacgttat tttaaaaagt
    11461 tgattcaggc ttatcgtgag attggaagcg aagcgggcca tgctgacaaa gacttgaaag
    11521 tgggagccca ttcttggggg tggattgcgg aagatggtga gcaggcggtg aaagattatt
    11581 tccatccgac taagcaagtg gtggatgcta tttccaaaga ccgtccgcac tggcaggaat
    11641 tgcgttatga gcaatatttg gatcaggtag ggtcaaatgg cgccatgttt gttggcaatc
    11701 cagatcaggt ggccgaaaaa ttgattcgca tgatagagga tttaggattg gactgcttca
    11761 tgcttcatct accgcttggt tctatgcctc atgaccaggt tctgagagct attgaactct
    11821 tcggaacaca agttgcaccc aaagtacgag cttattttgc tatgaaagag gcttaataaa
    11881 aaatcctaat caagtttatt aggacaacaa atattgtacg aaaattaatc ccccgagcta
    11941 taatgcttgg gggatttttc tataggaggg ctagtttagt tctctttttt attttttagt
    12001 aagaaaccga gaccaaggag cgcaaggagg ctgattcctg caaataggag tttgttttcg
    12061 tttttgattc ctgtatttgg aagttcagct tgtctatctg gggttgcagg tatatttttc
    12121 ttgctagagt tttctaccga ttcgttttga accgcttctt ttacagatga tgcttgtgct
    12181 tcttgtgctg gttgttcagc ttgtgaatca gctgtagctt ggctaggttt attttccgca
    12241 tcagctactt tggcatctgg tttagtagaa tcaggtctag cttctggagc tgggtcttga
    12301 tatgcagggt gagaagcttc attttgatgt tcaggcttgg aagggattga gtctggtttc
    12361 tcttctgcag ttgactcatg gctagttcca ttttgagaga ctcgaagaac agtagctgta
    12421 agggcattca atttcaagcc tttttcagtc cattcaagtc ctttcgggtt ggcaatttcg
    12481 actgatcctg cttggttttc atctgccaaa acttccgcat ttcttagatg tgcaaaggca
    12541 gttcccaaat taaattcgcg agctttttca tccgcattga caaagactgc gtagatatcg
    12601 ccgtttggag cagtgatttg gtagccaatc actacatcct ctttttccac accattttgg
    12661 cctgggacag tgatgaggtg gacacggtct ttgatatctt gaagactctt aagtcggaag
    12721 gcatctgtag attgacgaag ggcaatcaaa cctttcatat agtcacggct cttgacattt
    12781 tcaggataag ctttaccatc tgtagcctta gtccagtcaa acttgttgac tgcatcgcta
    12841 gaatcgtaag aatcatggat gaagtaagga tagtcaaatg ggttgccgtc cttatcacgc
    12901 aacaagtgag atttgtttgg aaccttatcc tctgctactg gagtcttgta ggctgggtca
    12961 cggaattgtt tagtacgtcc atattcctga ccggagtgga taaatggagt tccttgagct
    13021 gtcaagacca tgagatttcc aagtcgtaaa cgacggtgga tttcagcata gttctcagcc
    13081 ttgcttgggt cttttttgat agactgggca atgatgtcaa agagggtcaa gttatcatgg
    13141 gctgctatgt attggatgac atctccaggg ctgtcagctt caaagttagt tggttgagca
    13201 atgagatttt taaagatggt gttgacatca cgcttgccac ctgtgataaa ggcaggttga
    13261 ccttcgtttg gataaccaga tttgaggttg ttacggatgt catctgaaaa gacagcgaca
    13321 gtatcggtat gtttcatcca atcttggtca gcagctttag taggcatgtt ttcatcaccg
    13381 gcataggttc tccaaccttc accaagcatg atgaggtttg gattgagggc gcgtgcagcc
    13441 ttgtaagctt cttcgataga agcggcgtca tggtctccca tcatatcgaa gcggaagcca
    13501 tccactttgt aggtatcaac taggtatttg atagagtcaa ctaggagccg tttggtcata
    13561 tggtgggttg tccccaagcg tccaccacca aagctagttc gaggtgtgcc atctgcatcc
    13621 ataaagtggt agtagtttgg ctctaggtct tcaaagatat cgactttggc tgtgtggtta
    13681 taaacgacat ctaggatagc tcccatacca cgtttgtgga tttcgttgat gaggttttta
    13741 aattctgcga ttcgtttttc tggattctta ggatcgcttg agtacatacc agtcaaggag
    13801 aagtagtttt gagggtcata tccccagttg tagttgctgt tgcttgaagc gtagtcagac
    13861 aagcgttcat ggtttttcaa ttcattgaca aagtagtaag acaagactgg aaggagctgg
    13921 atatgggtta cacccaagtc tttgagatag tctagttttt caatgaaggc ttcaaaagtc
    13981 ccaaatggtt tggtcaagtc ttttgcaatg gcaggatctg aagtgaagtc acgcacatga
    14041 gcttcgtaga taacggcgtc ttcacgagtc ttgaaattgt gaatcttacc ataagtcaag
    14101 tcttgaggtc cgagtttagc tggatctaca aaggcggctt tagccacttt atgggcatcg
    14161 tcaatcttgg catcgtcgct attccaagca gcaagagatt tagcgtaagg atcgagtgca
    14221 agaacagttt taccttgacg ctcgatttgg tattgataat agtatccagt gaaatctgtg
    14281 attccgagtt tgtttgtgct gtctagagtt tgtttccaag ttcctctttc ccctttttca
    14341 agagcgacag ttccaactac tttgtcaggg tcattcttgt cgtagacaac aacagaaacc
    14401 ttatcagcac ttggtgacca aagggtcaaa tcaacttgtt ttccttcttc ttttaggtca
    14461 gctcccagtt tgccatcata gctgtatgtc tcatctttca ggcgccagct tgttttggta
    14521 gtgaattggt cggaattgta gctaacagta taaggatgtt ttgtgtcaga gaaatctccg
    14581 ctgtaggtca ctttcttacc agcttcatcg attgcaacat cggtaatagt taccttgttt
    14641 cctaggtgat tagtgatgtt ggagtgtttg aggatatctt cttttttagc accgacaagt
    14701 gttgaaaagc tactttcaat gctagaagtg cctacgtgtt gggctcctgt catacggata
    14761 tcatggacat agtatggatt tgtgtaaatc gattcatcat cgtcttttag gaaaatttgg
    14821 ctatgatttt tcaaatctgt gaacttataa ttttctttac ggattttcac gtcgtctcct
    14881 tgtttgctct catctagtaa taaaaatcca aattctcttg cggcttcatt aagaggaatg
    14941 tcgatatagc ggccatattt gcctgtagcc gtaaagtctg ttccgtcagg ccattgagcg
    15001 ctacttggat tttttacatc tccccagtac cagagagatt tcttgtcata gttgccatct
    15061 gtgcggtagt agttgacgcg aacagttcct gcaggctgtg gctcgtaaga gaaaaccttg
    15121 taatcttggt ctaaccaagc ctcgttcatt tttggaacta gtttttctac agatttatcg
    15181 ccggttagat tttttccagc tgtattgttg atgaggaagc taattttctt ggcttgttct
    15241 ccctttaatt tgacatctag gtaatagccg tagtcatctt tcttggcatc cttgaaggac
    15301 aaagctccgt ttggccagtt ttcagatggt ttttcaacat cgtcccaagt ccatagtcct
    15361 tgagcatcct tgttttcttc aggaagtttt ttgacatgga tacggaagta gttatcttcg
    15421 atgggctctt ctgccttagt ttcagttgtt actggactag tagaagtagt tggctctgtt
    15481 gcaggttttt cttctgtttt ctctagggaa gcgtttgcat ttctaggtga agaaatatca
    15541 ctttcgttct taccagagcc agctgctgta tcagtaagag gttgggcaag ggtggttgta
    15601 tttctgttgt tgggttcgtt agtggttggt gtactttcgt cggatgagat agttggtgta
    15661 gccatggcaa gcaagacaag gcttgcgccg ataaggacag aaccagttcc atttttgagg
    15721 gaacggatgc tgtagaccat ttttttctca gtgtgagatg gtgtttttct cataatgatt
    15781 ctccaatcaa taaatttcta tatcagtata gcatgtagta aaataatatg caagcgtttt
    15841 ctttgatttg tgaaaaaaat tcttactttt gagaagtttt ctgtactatt ttatatatca
    15901 gtataaatga cggagataaa aatgtcacat aagccaagcc gtattattag gaagatgatg
    15961 tgttcccagc tatttttatt cagaaattga tctctaacat taccaataaa aatgaaaaat
    16021 atcttgacaa gcaagggaaa attttgttac aatgatagac ggtacttttt acttttggtc
    16081 tctcaagagt gtacagggac gtgctgacaa atgttgcaaa agtacacaca gatgatatct
    16141 gtcaccaagt gtatcatcac caaaaataaa aaaacacagg agaatgtaga tgcctacaat
    16201 taaccaattg gttcgcaaac cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt
    16261 gaacgttggt tacaatagtc ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg
    16321 tggtgttgca actcgtgttg gaacaatgac acctaaaaaa cctaactcag cccttcgtaa
    16381 attcgctcgt gtacgtttga gcaaccttat cgaagttact gcctacatcc caggtatcgg
    16441 acacaacttg caagagcaca gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc
    16501 aggggtacgt taccatatcg tccgtggtgc acttgatact gcaggtgtta acgatcgtaa
    16561 acaaggccgt tctaaatacg gtactaaacg tccaaaagca taaggaaagg ggataaagag
    16621 aaatgagtcg taaaaataga gctccaaaac gtgacgtatt gccagatccg ctatacaatt
    16681 cacaactagt tactcgtctt atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg
    16741 cttcaatcgt ttacggtgct tttgagcaaa tcaaagaagc tactggcaac gatgcacttg
    16801 aagtatttga aacagctatg gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg
    16861 ttggtggttc taactaccaa gtcccagttg aagttcgtcc agaacgtcgt acaacacttg
    16921 gacttcgttg gttggtaaca atcgctcgtc ttcgtggtga acacacaatg caagaccgtc
    16981 ttgcaaaaga aatcttggat gctgctaaca acactggtgc agctgttaag aaacgtgaag
    17041 atactcaccg tatggctgaa gctaaccgtg cattcgcaca cttccgttgg taagatagga
    17101 tgcgaaagcg ttaagaaagt cccagagaaa atagggaatc gaagcaggtt gcggttgcaa
    17161 ccaatgagat tcatcttttt ctccagactt ttagcttgag ctcaactaaa tcatgatgct
    17221 aggcacggta aggatgcaag gtgaaaatag gaaactgacg cagtattcaa cgaatacaag
    17281 gagttttatc tttttcacgc agcatcccgt tccagctcac atcggctaac taactttagc
    17341 ccgggttcaa attagctaaa tcgattagta ttagctataa ctcagcttac catctagtaa
    17401 gttgaaacca acaatagcat gaaaacattg agaacgggta ggtcctgcct atccgttttt
    17461 attaaaatcg tgttataata gaatagaaat caaaaataaa taggagaaac aaacctcatg
    17521 gcacgcgaat tttcacttga aaaaactcgt aatatcggta tcatggctca cgtcgatgcc
    17581 ggtaaaacaa caactactga gcgtattctt tactacactg gtaaaatcca caaaatcggt
    17641 gaaactcacg aaggtgcgtc acaaatggac tggatggagc aagagcaaga acgtggtatc
    17701 acgatcacat ctgctgcgac aacagctcaa tggaacaacc accgcgtaaa catcatcgac
    17761 acaccaggac acgtggactt cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt
    17821 gcggttaccg ttcttgactc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    17881 caagcaactg agtacggagt tccacgtatc gtatttgcca acaaaatgga caaaatcggt
    17941 gctgacttcc tttactctgt aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca
    18001 atccaattgc caatcggttc tgaagatgac ttccgtggta tcattgactt gatcaagatg
    18061 aaagctgaaa tctatactaa cgaccttggt acggatatcc ttgaagaaga catcccagct
    18121 gaataccttg accaagctca agaataccgt gaaaaattga ttgaagcagt tgctgaaact
    18181 gacgaagaat tgatgatgaa atacctcgaa ggtgaagaaa tcactaacga agaattgaaa
    18241 gctggtatcc gtaaagcgac tatcaacgtt gaattctttc cagtattgtg tggttcagcc
    18301 ttcaaaaaca aaggtgttca attgatgctt gatgcggtta tcgactacct tccaagccca
    18361 cttgacatcc cagcaatcaa aggtattaac ccagatacag acgctgaaga aactcgtcca
    18421 gcatctgacg aagagccatt tgcagctctt gccttcaaga tcatgactga cccattcgta
    18481 ggtcgtttga cattcttccg tgtttactca ggtgttcttc aatcaggttc atacgtattg
    18541 aatacttcta aaggtaaacg tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc
    18601 cgtcaagaaa tcgacactgt ttactcaggt gatatcgctg ctgccgttgg tttgaaagat
    18661 actacaactg gtgactcatt gacagatgaa aaatctaaaa tcatccttga gtcaatcaac
    18721 gttccagaac cagttatcca attgatggtt gagccaaaat ctaaagctga ccaagacaag
    18781 atgggtatcg cccttcaaaa attggctgaa gaagatccaa cattccgcgt tgaaacaaac
    18841 gttgaaactg gtgaaacagt tatctcaggt atgggtgaac ttcaccttga cgtccttgtt
    18901 gatcgtatgc gtcgtgagtt caaagttgaa gcgaacgtag gtgctcctca agtatcttac
    18961 cgtgaaacat tccgcgcttc tacccaagca cgtggattct tcaaacgtca gtctggtggt
    19021 aaaggtcaat tcggtgatgt gtggattgaa tttactccaa acgaagaagg taaaggattc
    19081 gaattcgaaa acgcaatcgt cggtggtgtg gttcctcgtg aatttatccc agcggttgaa
    19141 aaaggtttgg tagaatctat ggctaacggt gttcttgcag gttacccaat ggttgacgtt
    19201 aaagctaagc tttatgatgg ttcatatcac gatgtcgact catctgaaac tgccttcaag
    19261 attgcggctt cactttccct taaagaagct gctaaatcag cacaaccagc tatccttgaa
    19321 ccaatgatgc ttgtaacaat cactgttcca gaagaaaacc ttggtgatgt tatgggtcac
    19381 gtaactgctc gtcgtggacg tgtagatggt atggaagcac acggtaacag ccaaatcgtt
    19441 cgtgcttacg ttccacttgc tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct
    19501 caaggacgtg gtacattcat gatggtattt gaccactacg aagatgtacc taagtcagta
    19561 caagaagaaa ttattaagaa aaataaaggt gaagactaat ccgtcctcac tctagaagga
    19621 agtcacttag tggcttcctt ttgtctttag aaaatacctc taaatatggt aaaatagtag
    19681 aagaataatg tgaggaaaat gaatgtcaaa tagttttgaa attttgatga atcaattggg
    19741 gatgcctgct gaaatgagac aggctcctgc tttagcacag gccaatattg agcgagttgt
    19801 ggttcataaa attagtaagg tatgggagtt tcattttgta ttttctaata ttttaccgat
    19861 tgaaatcttt ttagaattaa agaaaggttt gagcgaagaa ttttctaaga caggcaataa
    19921 agctgttttt gaaattaagg ctcggtctca agaattttca aatcagctct tgcagtccta
    19981 ctatagggag gctttctctg aaggtccatg tgctagtcaa ggttttaagt tcctttatca
    20041 aaatttgcaa gttcgtgctg agggtaatca gctatttatt gaaggatctg aagcgattga
    20101 taaggaacat tttaagaaga atcatcttcc taatttagct aaacaacttg aaaagtttgg
    20161 ttttccaact tttaactgtc aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga
    20221 ggcctttcat gctgaaaatg agcagattgt tcaagctgcc aatgaggaag cgctccgtgc
    20281 tatggaacaa ctggagcaga tggcacctcc tccagcggaa gagaaaccag tctttgattt
    20341 tcaagcgaaa aaagctgcag ctaaacccaa gctggataag gcggagatta ctcctatgat
    20401 cgaagtgacg acagaggaaa atcgtctggt atttgaaggg gttgtttttg atgtggagca
    20461 aaaagtgact agaacaggtc gtgttttaat caactttaaa atgacggact atacttcaag
    20521 tttttctatg caaaagtggg ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa
    20581 gaagaattct tggctccgag ttcgagggaa tgtggagatg aataacttca cacgcgattt
    20641 gactatgaac gtacaggatc tgcaggaagt tgttcactat gagcggaagg atttgatgcc
    20701 agaaggtgag cgtcgggttg agtttcatgc tcatactaac atgtcgacta tggatgcttt
    20761 gccagaggtc gaagagattg ttgcaacagc tgctaagtgg ggacacaagg cggttgctat
    20821 cacagaccat gggaatgtcc agtcctttcc acatggctat aaggcggcta agaaagcggg
    20881 aatccagctg atctatggga tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta
    20941 taacgaagtg gagatggact tatcagaagc aacctacgtg gtctttgacg tggaaacgac
    21001 gggactttca gctatctata atgacttgat tcaggttgcg gcctctaaga tgtacaaggg
    21061 gaatgttatt gctgaatttg atgaatttat caatcctggg catcccttgt cagcttttac
    21121 tacagagtta actggaatta cagatgatca tgtcaaaaat gccaaaccac tagaacaagt
    21181 tttgcaagaa ttccaagaat tttgcaagga tacggtccta gttgcccaca atgctacctt
    21241 tgacgttggc tttatgaatg ctaattatga gcgtcatgat cttccaaaga ttagtcagcc
    21301 agttattgat acgctggagt ttgctagaaa cctctatcct gagtataaac gccatggttt
    21361 ggggcctttg accaagcgtt ttggtgtggc cttggaacat caccacatgg ccaactacga
    21421 tgcggaagcg actggtcgtc tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg
    21481 tgtgaccgat ttagctagac tcaacattga tctaatcagt ccagattctt acaaaaaagc
    21541 tcggatcaag catgcgacca tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa
    21601 gctggtttcc ttgtctaata ccaagtattt tgaaggagtg ccacggattc cgagaacggt
    21661 tctagatgcc catcgagagg gcttgatttt aggttcagcc tgttcagagg gtgaagtttt
    21721 tgacgtggtc gtttctcaag gtgtggatgc ggcggttgag gtggccaagt attatgactt
    21781 tatcgaggtc atgccaccgg ctatctatgc gcccttgatt gccaaagagc aggtcaagga
    21841 tatggaggaa ctccagacca ttatcaagag tttgatagag gttggagatc gccttggcaa
    21901 gcctgttctg gctacgggaa atgttcacta tatcgaaccg gaagaagaga tttatcgtga
    21961 aattatcgtc cgtagtttgg gacagggtgc gatgattaac cgaactatcg gtcatggtga
    22021 acatgcccaa ccagcgccac ttccaaaggc tcattttcga acgactaatg agatgttgga
    22081 tgaatttgcc tttttgggag aggaactggc tcgtaaactg gttattgaaa acaccaatgc
    22141 cttggcagaa atatttgaac ccgttgaagt cgttaagggt gacttgtata cgcctttcat
    22201 cgacaaggct gaagaaacag ttgctgagtt gacctataag aaagcttttg agatttatgg
    22261 aaatccgctg ccagatattg ttgatttgcg gattgaaaaa gaattaacat ccatactggg
    22321 gaatggattt gctgtgattt atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg
    22381 gggttatttg gttggttctc gtgggtctgt cggatctagt ttcgttgcga ccatgattgg
    22441 gattacggag gtcaatcctc tctctcctca ctatgtctgt ggtcagtgtc agtacagtga
    22501 gtttatcaca gatggttcgt acggttcagg atttgatatg ccccataagg actgtccaaa
    22561 ctgtggtcac aaactcagta aaaacggaca ggatattccg tttgagacct tccttggttt
    22621 tgatggggat aaggttcctg atattgactt gaacttctcg ggagaagatc agcctagcgc
    22681 ccacttggat gtgcgtgata tctttggtga agaatatgcc ttccgtgcgg gaacggttgg
    22741 tacggtagct gccaagactg cctatggatt tgtcaagggt tacgagcgag attatggcaa
    22801 gttttatcgt gatgcagaag tagaacgcct cgctcaagga gcggcgggtg tcaagcggac
    22861 aacaggccaa cacccggggg gaatcgttgt tattccgaac tacatggatg tctacgattt
    22921 tacgcctgtc cagtatccag cagatgatgt cacggctgaa tggcagacca ctcactttaa
    22981 cttccacgat atcgatgaga acgtcctcaa actcgatgta ctgggacatg atgatccgac
    23041 catgattcga aaacttcagg atttgtctgg tattgaccct aataaaattc ctatggatga
    23101 cgaaggcgtg atggcactct tttctgggac tgatgtacta ggggtaacac ctgaacaaat
    23161 tggaacgcct acgggtatgt tggggattcc agagtttgga acaaatttcg tacgtggaat
    23221 ggtagacgaa acccatccga caacctttgc ggaattgctt cagctgtctg gtctgtccca
    23281 cggtactgac gtttggttgg ggaatgctca ggatctgatt aagcaaggaa tagcggacct
    23341 atcgactgtt atcggttgtc gggacgacat catggtttac ctcatgcatg cgggtctgga
    23401 acctaagatg gcttttacca ttatggaacg ggtacgtaag ggtttgtggc taaagatttc
    23461 agaagaggag agaaatggct atatcgaagc catgaaggct aataaggtgc cagagtggta
    23521 tatcgaatcc tgtgggaaaa ttaagtacat gttccctaag gcccatgcgg cagcctacgt
    23581 tatgatggcc ttgcgtgtag cttacttcaa ggttcaccat cctatttatt actactgtgc
    23641 ttacttctcc attcgtgcta aggcttttga tatcaagacc atgggtgcgg gcttggaggt
    23701 catcaagcgc agaatggaag aaatctctga aaaacggaag aacaatgaag cctctaatgt
    23761 ggaaatcgat ctctatacaa ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa
    23821 gtttggtaaa ttagatctct accgtagtca ggcgacagag ttcctcatcg acggggatac
    23881 ccttatccca ccatttgtag caatggatgg tctgggagag aacgttgcca agcaactggt
    23941 gcgggcgcgt gaagagggag aattcctctc taaaacagaa ctacgcaagc gtggtggact
    24001 ctcatcaacc ttggttgaaa agatggatga gatgggtatt cttggaaata tgccagagga
    24061 taaccagttg agtttgtttg atgagttgtt ttaaaaaatt gcttaataat ctattaaaag
    24121 aggctaacgt atatccaata gatttacatt agctttcttt tttgttaaaa tagtctatgg
    24181 aaagagggtg agagtatgtc aaagatgagt ataagcatcc gtctggatag tgaggttaag
    24241 gagcaggccc aacaggtgtt tagtaatctg ggaatggata tgacaacagc tattaatatt
    24301 ttccttcgtc aggcaattca atatcaggga ttaccttttg atgttagact agacgaaaat
    24361 cggaagttgc tccaagcgtt aacggattta gaccaaaatc gtaatatgag ccagtctttt
    24421 gaatcagtct cagatttgat ggaggactta cgtgcttaag attcgttatc ataaacagtt
    24481 taaaaaagat tttaagttgg ctatgaagcg tggtttgagg gcagaattat tagaagaagt
    24541 tttgaatttt ctggttcaag aaaaagaaca tcctgccaga tatcgtgatc attcattgac
    24601 ggcatccaag cattttcaag gagttcgtga atgccatacc cagccagatt ggcttttggt
    24661 ttataaagta gacaagtcgg aattgatttt aaatttgctg aggacaggca gtcacagtga
    24721 tttattttaa tctattttaa gggggttctc atgaaactaa gaatatttgc ggaagataag
    24781 ccggctaaga aggtatttga atatcaatta gaacttgctg atcgtacaat tcttctatcg
    24841 acagcactct tgccaggtgc tattgcttta gcaggaatct tttctgcttt gaaagaaaaa
    24901 taaaaataga aaagagaaaa cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa
    24961 tatgcgaaat tgttggttgc gaacggaatt aacgtgcaac ctggtcacac tttggctctc
    25021 tctattgatg tggagcaacg tgaattggca catctaatcg tgaaagaagc ttatgccttg
    25081 ggtgcgcatg aggtcatcgt tcagtggaca gatgatgtga ttaaccgtga gaaattcctc
    25141 catgccccga tggagcgttt ggacaatgtg ccagaataca agattgctga gatgaactat
    25201 cttttggaga acaaggctag ccgtcttggg gttcgttcat ctgatccagg tgccttgaac
    25261 ggagtggacg ctgacaagct ttcagcttct gctaaagcta tgggacttgc catgaagcca
    25321 atgcgaatcg caactcaatc taacaaggtt agctggactg tagcagccgc tgctggactt
    25381 gagtgggcta agaaagtctt cccaaatgct gcgagcgacg aagaagcagt tgatttcctt
    25441 tgggatcaaa tcttcaaaac ttgtcgtgtc tacgaagcag atcctgttaa ggcttgggag
    25501 gaacatgcag ccattctcaa gagcaaggcc gatatgctta ataaggagca attttcagcc
    25561 cttcactaca cagcgccagg aacagattta acacttggtt tgccaaagaa ccacgtttgg
    25621 gaatcagctg gtgctgtcaa tgcacagggc gaagaattct tgccaaatat gccgacagaa
    25681 gaggtcttca cagcgcctga cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg
    25741 cttagctaca acggaaatat cattgaaggc attaaggtga cctttaagga tggacaaatc
    25801 gtagatatca ctgctgagaa gggtgatcag gttatgaaag accttgtctt tgaaaatgcg
    25861 ggtgcgcgtg ccttgggtga atgtgccttg gtaccagatc caagtccaat ttctcagtca
    25921 ggcattatct tctttaacac ccttttcgat gaaaatgcgt caaaccactt ggctatcggt
    25981 gcagcctatg cgactagcgt tgttgatgga gcggagatga gcgaagagga gcttgaagct
    26041 gcagggctta accgttcaga tgttcacgta gactttatga ttggttctaa ccaaatggat
    26101 atcgatggta ttcgtgagga tggaacgcgg gtacctcttt tccgtaatgg gaattgggca
    26161 aattaaggag ataatatgtt aggaagtatg ttcgttggtc tcctagtggg atttttagca
    26221 ggtgctatga ccaatcgtgg agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg
    26281 atcggagcct ttctaggtca cttgctcttt ggaacttggg ggccagtttt atcaggaaca
    26341 gctattatcc cagcgatttt aggagccatg attgttttag ctattttttg gagacgagga
    26401 agttaatttc ctaaatctga tactcaatga aaatcaaaga gcaaactagg aaactagccg
    26461 caggttgctc aaagcactgc tttgaggttg cagatagaac tgacgaagtc agtaacatat
    26521 atacggcaag gcgacgttga cgcggtttga agagattttt gaagagtatg aaacgaaaag
    26581 gaggttggtc attgtaccag cctccttttg atatgatata atagttctat gagattagat
    26641 aaatttttag ttgcctgtgc ggtgggaagt cggactgagg tcaaaaactt gctcaaggct
    26701 gggcgcgtga ctgtaaatgg taaaaaagaa aaatcagcta aattgcagat tgatgaaaaa
    26761 atagatgaga ttcgctttga tgggcaagtg ttggagtatg aagagtttgt ctactacatg
    26821 atgaacaagc ccaaaggagt tatctcagcg actgaggatc ccaagcacag aaccgttctg
    26881 gacttgttgg atgacttggc gcggagtaag gaagttttcc cagtaggacg cttggatatt
    26941 gacacgcatg gtcttttgct cttgaccaat gatggtcagc tggctcatgt tcttctttcg
    27001 cccaagcgtc atgtggataa gacttatctg gcacaagtca agggaatcat gacccaagaa
    27061 gatgtggaga catttgctga gggtattcct ctcaaagact ttacctgtca acccgctata
    27121 ctggagcttg tatccataga tacagaaaag aatcagagcc aaatccgtgt gaccattgca
    27181 gaagggaagt ttcatcagat caagcgtatg gtgggctact gtggcaagga agtggtggac
    27241 ttgcaacgat tgactatggg aactttagta ttagatgaga acctggaacg aggggaatgg
    27301 cgtcgcttga ccaaggaaga attagaaatt ctccgcgcta atattattta atttagttta
    27361 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg
    27421 gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtataaattc gaaaaaggtg
    27481 agggagaatg tccttacctt ttatgttttt tagttgcttc ctttgtgaaa agagttataa
    27541 tagactgtag aataaaagga ggaatctatg aacgcgattc aagaatcatt tactgataaa
    27601 ctatttgcca actatgaagc aaatgtaaaa taccaagcga ttgaaaatgc tgccagccac
    27661 aacggaattt ttgcagctct ggaacgtcgc caaagccatg tagacaacac acctgttttc
    27721 tcattggatt taaccaagga caaggtcact aaccagaaag cgtctggtcg ttgctggatg
    27781 tttgcggctc tcaacacctt ccgccacaaa ctcatctcgc aatacaaatt ggagaacttt
    27841 gagttgtcac aagcccacac tttcttctgg gataagtatg agaaatcaaa ctggttcttg
    27901 gagcaagtca ttgcgacttc agaccaagaa ttgactagcc gcaaggttag cttcttactc
    27961 caaacacctc aacaagatgg tggtcaatgg gatatggtcg tttccctctt tgaaaaatac
    28021 ggtgtcgtgc ctaagtcagt ttatcctgag tctgtttcat ctagcagcag tcgtgagcta
    28081 aatgcgatcc ttaataaatt gcttcgtcaa gatgctcaaa tcttgcgtga tttgcttgtt
    28141 tctggtgcag atcaagtgac tgttcaagct aagaaagaag acctcttgca agaaatcttt
    28201 aactttcttg ctatgtcatt aggacttcca ccacgcaagt ttgactttgc ttatcgcgat
    28261 aaagataaca actacaaaag tgaaaaagga atcacaccac aagagttcta caagaaatat
    28321 gtcaatcttc ctttagaaga ctacgtttct gttatcaatg ctccaactgc tgataaacct
    28381 tacggaaaat cttacacagt tgagatgttg gggaatgtgg ttggtagccg tgcagttcgt
    28441 tacatcaacg ttccaatgga gcgcttgaaa gaattggcga ttgcccaaat gcaagcaggt
    28501 gagactgttt ggtttggttc tgatgtcggc cagctcagta accgtaaggc tggaatcctt
    28561 gcgacagatg tttatgactt tgaatcaagc atggacatta aacttactca agacaaggct
    28621 ggacgtttgg actatagcga aagcttgatg acccacgcca tggtcttgac aggtgttgac
    28681 ttggacgaaa atggtaaatc aaccaagtgg aaggttgaaa actcatgggg agacaaggtc
    28741 ggtacagatg gttactttgt tgcctcagac gcttggatgg acgaatacac ataccaaatc
    28801 gttgttcgta aggaattgct gacagcagaa gaacaagctg cctatggagc agaaccaatt
    28861 gtacttgcac catgggatcc aatgggagcc ttggctaaat aaaagcatag aaaaaaggaa
    28921 tcagatttta gaacctggtt cctttttagt tgcttgatta catgatgtga agaacatgtg
    28981 ccacaatacc cactgcgaag agggcaagga taatagtgat tggagatact tttttcttaa
    29041 gtaagtacat gcaaagtaaa gtaaggagta gtcctgataa tccaggaatc aacatatcca
    29101 agttttgttg gaaagtagta actttttcag gagtttgaga caatccttgt cctacttgtg
    29161 cgaatgcttc ttggatacct ttagaccctt ctggcaattt atcccaatgg atataagcct
    29221 tttcatctag ttgaacttta gaaacatcga aagcaaattt aatatttacc cagcgttgaa
    29281 caaggacagc aagaatgaac atcccaagga tagaagctcc tttagtgata tcttgaagga
    29341 taccaccaga catatcttta gtgatttctg atccagcctt gtatccaatc tcttgaacat
    29401 accacaagaa tgacatacga atcaagttcc atgcaacaaa gaagaggagt ggccccaaga
    29461 tattgccagt aagggcaagt gaagcaccga gagatccaag gattgggcgt actgtaaacc
    29521 agaatactgg gtcaccgata ccagcaagag gtcccatcat accgatttta accccttgga
    29581 tagcagcgtc atcgatttcc acaccgttag cacgttcttc ttcaagcgca agagtaaccc
    29641 ccatgactgg agcagctacg tatggatgag tgttgaagaa ctcaaggtga cgctcaagag
    29701 cagcgatttg atcttctttt ttagtataga gttttttgat agctggaatg agtgtataag
    29761 cccagcccaa gttttgcatc cgttcaaagt tccaagaccc ttgtaagaag gttgaacgcc
    29821 accaaacttt tttacgatct gatttagtta attgaagttt ttcagtcatg atgttttcag
    29881 tcctttctta tcttagtagt cttctaggat atcgccgatt gggtcgttag aagttgcggc
    29941 tcctccgcca ccatttccac cagttttaga aaggtgaagg tagataagag cgatagcaac
    30001 accgatagca ccgaatccga ttagagtaat atctgacaca gcagcgagaa cgaaaccaag
    30061 agcgaagaat ggccatactt cacgagttgc catcatgttg ataaccatgg cgtaaccaac
    30121 ggcaacgacc ataccaccac cgatagccat accatctttg agccagtctg gcatggcact
    30181 aaggatactt tgtacagttt cagttggtac cataaggaga agagctgcag gaagcgcgat
    30241 acgaagtcct tggaaaagta gcgcaatgaa atgcgcacgc tccacagcgc cgaagtcacc
    30301 ttttttagcg gcagcatctg cagtatgaac caaaccaact gaaattgtac gaacaatcat
    30361 tgtcaagaaa agtccagcta cggcaagagg gatagcaacc gcttgggcaa caccgatacc
    30421 agtcttggta aagtcaccac caagaaccat gataatggca gcagcgacag aagcaagtgc
    30481 agcatcagga gcgatagcag caccgatatt tgaccaacca agggcaatca tttgaagcga
    30541 tccaccgagg ataatccctg cttccaagtg acctgttaca agcccaataa gggtacaggc
    30601 tacaagtggt tggtgaaatt ggaactggtc gaggatgcct tcaagacctg caaagaaggc
    30661 tacaacgatt actaaaacca tagaaataat agacatgttt aaaatccttt cataaataat
    30721 ggcttatttg acattggctt tgttaatcaa gtcaaacaaa tcttttttag aatcatttgg
    30781 tactttacgg acatcaaatt caacacccaa gtcacgcatt ttttcaaatg tagcaacatc
    30841 ttctttgtcc atagacaaaa cggtattgac caatgtttta cctgttgagt gagccataga
    30901 accaacgtta agagtcttga ttggcacgcc gccttcgatg gcacgaaggg catcttgagg
    30961 tgtttcaaac aagataaggg catgtgtttc tccaaaacgt gggtcttttg aaatctcaat
    31021 cagtttttga attggaacca cgttagcctt gacattacct ggagctgctt gtttaatcaa
    31081 ttctttacga aggtcgtctt tagccacgtt atctgaagca acgatgatac gatttgcttt
    31141 tgaatctgga gtccaagcag ttgcaacctg accgtgaagt agacgtgtgt caagacgggc
    31201 aagattgatt ttcaatttac cgtctccgat aacagttcct tctgggatag cagtttgggc
    31261 aactggagca gctgcagcgc ttgcaacttc ttcaactgga tttagctctt ctggaagagc
    31321 tttgatgcca tctttggctt ctttaatgat attagcagcg actttttcta cacctgcagc
    31381 agcgtccatg aggcgctctg tgtaggcttg aatcaacatc ggtaagttaa gtcctgtgat
    31441 gatggcaaac ttacgctcag gattttctcc catcacgcga ctagcttggt taaatggaga
    31501 accactccaa aggtcagcca aaactagaac ctcatcttct gcgtcaaatg cagcaacagc
    31561 gttattaaac ttagcgtata gatcatcagg accttcattt ggcataaagg tcacaacttg
    31621 aaccttttct tgttcaccaa agatcataga tcctgactga tgaataccag cagcaaattc
    31681 gccgtggctc gcaataatga ttccgatact cattattgtc attcctcctt ttttgtttta
    31741 gttttcattt aagaaaact
//
LOCUS       DAVHIU010000011        31379 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVHIU010000011 DAVHIU010000000
VERSION     DAVHIU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.011"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(314..727)
                     /gene="ndk"
                     /locus_tag="VVN06_000411"
     CDS             complement(314..727)
                     /gene="ndk"
                     /locus_tag="VVN06_000411"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5562020.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(838..4515)
                     /gene="rpoC"
                     /locus_tag="VVN06_000412"
     CDS             complement(838..4515)
                     /gene="rpoC"
                     /locus_tag="VVN06_000412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5562021.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQKHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISKLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4548..8159)
                     /gene="rpoB"
                     /locus_tag="VVN06_000413"
     CDS             complement(4548..8159)
                     /gene="rpoB"
                     /locus_tag="VVN06_000413"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5562022.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8575..8645
                     /locus_tag="VVN06_000414"
     tRNA            8575..8645
                     /locus_tag="VVN06_000414"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8607..8609,aa:Cys,seq:gca)
     gene            complement(8843..10174)
                     /locus_tag="VVN06_000415"
     CDS             complement(8843..10174)
                     /locus_tag="VVN06_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU5562023.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10465..11289)
                     /gene="endA"
                     /locus_tag="VVN06_000416"
     CDS             complement(10465..11289)
                     /gene="endA"
                     /locus_tag="VVN06_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU5562024.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11328..11516)
                     /locus_tag="VVN06_000417"
     CDS             complement(11328..11516)
                     /locus_tag="VVN06_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5562025.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11509..12792)
                     /gene="murA"
                     /locus_tag="VVN06_000418"
     CDS             complement(11509..12792)
                     /gene="murA"
                     /locus_tag="VVN06_000418"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5562026.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12816..13046)
                     /locus_tag="VVN06_000419"
     CDS             complement(12816..13046)
                     /locus_tag="VVN06_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU5562027.1"
                     /translation="MIQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13104..14141)
                     /locus_tag="VVN06_000420"
     CDS             complement(13104..14141)
                     /locus_tag="VVN06_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5562028.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFTPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14125..14613)
                     /gene="coaD"
                     /locus_tag="VVN06_000421"
     CDS             complement(14125..14613)
                     /gene="coaD"
                     /locus_tag="VVN06_000421"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5562029.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDMIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14603..15142)
                     /gene="rsmD"
                     /locus_tag="VVN06_000422"
     CDS             complement(14603..15142)
                     /gene="rsmD"
                     /locus_tag="VVN06_000422"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5562030.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15207..16199)
                     /locus_tag="VVN06_000423"
     CDS             complement(15207..16199)
                     /locus_tag="VVN06_000423"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU5562031.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16311..16994)
                     /locus_tag="VVN06_000424"
     CDS             complement(16311..16994)
                     /locus_tag="VVN06_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5562032.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(17050..17790)
                     /locus_tag="VVN06_000425"
     CDS             complement(17050..17790)
                     /locus_tag="VVN06_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5562033.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17824..18102
                     /locus_tag="VVN06_000426"
     CDS             17824..18102
                     /locus_tag="VVN06_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5562034.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18180..19106
                     /gene="yidC"
                     /locus_tag="VVN06_000427"
     CDS             18180..19106
                     /gene="yidC"
                     /locus_tag="VVN06_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5562035.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19181..19975)
                     /gene="pflA"
                     /locus_tag="VVN06_000428"
     CDS             complement(19181..19975)
                     /gene="pflA"
                     /locus_tag="VVN06_000428"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU5562036.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20101..21351)
                     /locus_tag="VVN06_000429"
     CDS             complement(20101..21351)
                     /locus_tag="VVN06_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5562037.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPEEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21418..22245)
                     /gene="purR"
                     /locus_tag="VVN06_000430"
     CDS             complement(21418..22245)
                     /gene="purR"
                     /locus_tag="VVN06_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU5562038.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22512..23453)
                     /locus_tag="VVN06_000431"
     CDS             complement(22512..23453)
                     /locus_tag="VVN06_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU5562039.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(23443..24699)
                     /locus_tag="VVN06_000432"
     CDS             complement(23443..24699)
                     /locus_tag="VVN06_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5562040.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDNLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24701..25363)
                     /locus_tag="VVN06_000433"
     CDS             complement(24701..25363)
                     /locus_tag="VVN06_000433"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU5562041.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25326..25982)
                     /locus_tag="VVN06_000434"
     CDS             complement(25326..25982)
                     /locus_tag="VVN06_000434"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5562042.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRQEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(25993..26871)
                     /gene="rsgA"
                     /locus_tag="VVN06_000435"
     CDS             complement(25993..26871)
                     /gene="rsgA"
                     /locus_tag="VVN06_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5562043.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(26873..27745)
                     /gene="rsmA"
                     /locus_tag="VVN06_000436"
     CDS             complement(26873..27745)
                     /gene="rsmA"
                     /locus_tag="VVN06_000436"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5562044.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28131..28289)
                     /locus_tag="VVN06_000437"
     CDS             complement(28131..28289)
                     /locus_tag="VVN06_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562045.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEG"
     gene            complement(28282..28923)
                     /locus_tag="VVN06_000438"
     CDS             complement(28282..28923)
                     /locus_tag="VVN06_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562046.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(28925..30955)
                     /locus_tag="VVN06_000439"
     CDS             complement(28925..30955)
                     /locus_tag="VVN06_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5562047.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGLSATALLPNYREMQEMEKASDKWSQSSDRYRLSFGW
                     SSAFADEEGMRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGVEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31223..>31379)
                     /locus_tag="VVN06_000440"
     CDS             complement(31223..>31379)
                     /locus_tag="VVN06_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator PlcR"
                     /protein_id="HEU5562048.1"
                     /translation="FLWKQALREERDFSLAESFYQSSKTFAQLIGDEFLVKKLTEEWQ
                     EDVKKYL"
BASE COUNT         8802 a         7371 c         6130 g         9076 t
ORIGIN      
        1 tagtcaattg aaacaagaac aggataaaag aaccttttgt gccatatttt tctcctttcg
       61 ctttacaatt ggattgaaca cctttattgt atcgcgtttg gagttttttt ggtataacct
      121 tcgacgcgca cccgcatagc gggtgttttt ttgtctcgca cctaacggag cgagacaaac
      181 taatagtcac ttaatcaaaa aacgcaccat atcaaaaact aaaaagtttg atatcatgcg
      241 tcatgtctta aactaattga ctatactttc tattcaaatg agcttttaac caattgattg
      301 agccaatcca ctcttaaaac caaagagcaa tttctcgctt agctgactct tctgaatctg
      361 aaccatgtac aacattttgg ataatctcat tttctccagc agcttttgca aaatcacctc
      421 gaatagtgcc tggtaaagct tcttctggac gagttgcacc catcatggtc cgccaagttt
      481 cgattacttt gggaccagaa atgacaccca caagaactgg acctgaagtc atgaattcac
      541 gaatcggtgg gtaaaaactc tgaccaacca agtcctgata gtgctggtca atcaactctt
      601 ctgaaacctg tgaacgaaac tccaattttt cgattgtaaa tccacgttgt tcgatgcgct
      661 ttaacacttc acccactagc cctcttttta caccatctgg tttgatgata aagaatgttt
      721 gttccatacc cgtctccttt gtcagcttct ttcttttatt ttaccacatt tcgtggaaaa
      781 atggagaaag ttttcagaag agagaatgag agaaccctcg ggttctctca ttctctctta
      841 ttctactgtt tcttccacag tttcaacggc agtatccaca actacttctg ttgtttcttc
      901 atttccttct tcctctactg gaggattaag gtattcttct tcgttgacag catgtggttc
      961 aaggttacgg taacgggcca taccagtacc agctgggatg atcttaccga tgataacatt
     1021 ttctttaagt ccaaggagat ggtctttctt accacggata gctgcgtcag taaggacacg
     1081 agttgtttcc tggaaggaag ccgctgacaa gaaactgttt gtttcaagtg aggctttggt
     1141 aattcccata aggactgggc gacctgtcgc tggaactcca cctgcgataa ggacatcttt
     1201 gttggcatct gtaaagtcat tgatatccat gagggtaccc atgagaagat ctgtatcacc
     1261 tggatccatg acacggactt tacggatcat ttgacgaacc attacctcga tgtgtttgtc
     1321 accgatttct accccttggc tacggtaaac tttttgtact tcaccgagaa ggtacgtttc
     1381 aactgacaag acatcacgaa ctgcaaggag acgttttggt tggatagaac cttctgtcag
     1441 agcagcacca cgcgctactt ggcccccaac ttcgacacgc atacgagctg taaatggtac
     1501 gacgtattca ccttcgccag tttcgccctt aacaaagact ttcttggtac gagttgaggc
     1561 atcttcttcg atagcagtaa cttgtccttt aacctctgtg ataaccgctt cccctttagg
     1621 attgcgggct tcaaagattt cttggacacg aggaagaccc tgagtgatat cggtatttga
     1681 ggcaacccca cccgtgtgga aggtacgcat tgtaagctgt gtaccaggtt ccccgataga
     1741 ttgggcagcg attgtaccaa ctgcttcacc aacttcaacc gcatcaccag tcgccaagtt
     1801 gataccgtaa caatgacggc agacaccatg acgagtgtta catgtaaata cagagcggat
     1861 agtcacttct tccacaccag cgttgacaat ttcacgcgcc ttgtcttctg taatcaactc
     1921 atttggacca atgatcactg caccagtttc tggatgttta acagttttct tagtgtaacg
     1981 accattgaga cgctcttcga gtgattcgat catctctttt ccttctgcga tagagcggat
     2041 caagagacca cgatctgttc cacagtcgtc ctcacggatg ataacgtctt gggcaacgtc
     2101 aaccaaacga cgagtcaagt aacctgagtc ggctgtctta agggccgtat cggtcatacc
     2161 tttacgcgca ccgtgagttg agaagaacat ttccaatacc gacaaacctt cgcggaagtt
     2221 tgaaaggatt ggcaattcca tgatacgtcc gttcggagcc gccatcagac cacgcatacc
     2281 ggcaagctgt gagaagtttg agatgttacc acgggctcca gagtccatca tcataacgat
     2341 tgggttctta ggatcttggt tggcaatcaa acgtttctca agtttttcac gggcagcacg
     2401 ccattcagct gtaacggcat tgtaacgctc gtcgtctgtg atcataccac gacggaattg
     2461 ttttgtgatt tgttctacac gtttgtgtga ttcttcaatg atttcagcct tgtcatcaac
     2521 gactgggata tcggcaatac ccactgtcaa tcctgcaaga gttgagtggt ggtaaccgag
     2581 gttcttcatg cggtcaagta gggcagaagt ttctgtcgta cggaaacgtt tgaagatttc
     2641 agcgatgata tttccaaggt ttttcttctt gaatggaggg ttgagttcaa gtttgctgat
     2701 tgcttccttg atatctccac caagtggcaa gaagtattta gctggaacgc cttctgtcaa
     2761 gttggcattg tttggttctt gcaagtatgg taggccctct ggcatgatgt cgttgaagag
     2821 aattttacca actgttgtaa gcaagacctt atgtttttgc tcttctgtcc aaggtttgtt
     2881 gaggctatct gttgcgatac caacacgtga gtgaaggtga acataaccat tacggtaggc
     2941 cataaccgct tcgtcacggt ctttgaagac cattccttca ccttcacgac cagcttcttc
     3001 catagtcaag tagtagttac ccaaaaccat gtcctgagat ggagtaacaa ctggtttacc
     3061 atctttcggg ttcaagatgt gctcagcagc gagcatcaag atacgagctt ctgcttgagc
     3121 ttcttctgaa agcggtacgt ggatggccat ttggtctccg tcaaagtcgg cattgtaggc
     3181 ttcacagaca agcgggtgca agcgaagggc cttaccatca atcaagactg gctcgaaggc
     3241 ttggataccc aaacggtgaa gggtcggtgc gcggttcaaa agcactgggt gttctttgat
     3301 cacttcttca aggatatccc agatacgctc atctccacgt tcgaccaagc gtttagccgc
     3361 tttgacgttt tgcacgatat cacgggcaac gatttcacgc atcacgaatg gtttaaagag
     3421 ctcaattgcc atttcacgtg gcacaccaca ttggtacatc ttaagagttg gaccaacggc
     3481 gataacggaa cgtcctgaga agtcaacccg tttaccgagc aagttttgac ggaagcgtcc
     3541 ttgtttccct ttgagcatgt ggctcaatga tttcagtgga cggctacctg gtcctgtgat
     3601 tggacgacca cgacgaccat tgtcaatcaa agcgtcaact gcttcttgaa gcatacgctt
     3661 ctcattttga acgataatac ctggtgcatt caactcaagc aaacgagcca aacggttgtt
     3721 acggttgata acacggcggt aaaggtcgtt caagtcagat gaggcaaaac ggccaccatc
     3781 caactgcaac attggacgaa gatctggtgg aataaccgga aggatgttaa gaatcatcca
     3841 ttcaggtttg tttccagact tgtaaaaggc atccaaaaca tccaaacgac ggatggcttt
     3901 gacacgtttt tgtccagtag ctgttttcaa ctcttctttg agttcagcaa tttctttttc
     3961 aagatctact tgtttcaaga ggtcttggat agcttcggca cccatcttgg caacgaatga
     4021 tccataacca tattcacgca agcgctcacg gtattcgcgc tctgtcatga tagacttgtg
     4081 ttcaagtggt gtatccttag gatcaatcac cacataggca gcaaagtaga taacttcctc
     4141 gagggcacga gggctcatat caagggtcaa gcccatacgg cttggaattc ccttgaagta
     4201 ccagatgtga gatacaggag ctttcaactc gatgtgcccc atacgctcac gacgaacttt
     4261 cgtacgcgtt acttcaaccc cacagcggtc acaaacaatc cctctgtaac gaatgcgttt
     4321 gtacttacca caagcacatt cccagtcttt tgtaggtcca aagataactt catcaaagag
     4381 accttcacgt tctggtttca aagtacggta attgattgtt tcaggttttt tgacttctcc
     4441 ataagaccat gaacggactt tacttggaga agctagggtg atttgcatac ttttaaaacg
     4501 atttacatca accactattt cttccctttc tattctaagt gaactgctta ttcttgttca
     4561 gcagcttctt ctgttgcttc cgcttttgtt gctttctcag cttcttcagc ttcaaaggct
     4621 gctttagcct cttgggctgc tttttcgcgg gctttttcaa ggtcatctac gtggatgaca
     4681 tcttcgtcca ttccttcatc caagtcgcga agttccactt cttggtcatc ttcgtctagg
     4741 acacgcatgt caagaccaag agattgcaat tctttgacaa gaactcggaa ggattctgga
     4801 acacctggtt ttggaattgg tttgcctttt gtaatagctt cataggcttt caaacgtccg
     4861 ttgatatcgt cagacttgta agtcaagatt tcttgaagga catttgacgc accgtaggct
     4921 tcaagagccc aaacctccat ctcaccgaaa cgttgtccac caaactgagc tttacctccg
     4981 agtggttgtt gggtaacagt tgagtaaggt ccgactgaac gcgcgtgcaa tttatcgtca
     5041 accatgtggt ggagtttgat catgtacatg actccaacag aaacacggtt atcaaatggt
     5101 tcaccagtac gtccatcgta aaggatcgtt ttggcatcgc tatccatacc tgcttcttta
     5161 acagttgacc aaagatcttc agaacttgct ccatcaaaga ctggtgtcgc aatgtgaata
     5221 ccaagagtac gagctgccat accaaggtga agctccataa cctgaccgat attcatacgt
     5281 gatggcaccc caagtgggtt caacatgatg tcgactggag ttccgtctgg aaggtaaggc
     5341 atgtcttcta caggaacgat acgagagaca acccctttgt ttccgtgacg tccggccatt
     5401 ttatctccga ccttaatctt acgtttttga gcgatgtaaa cacgaaccaa catgttaaca
     5461 cctgattgca actcatctcc atttacacgt gtaaagatct taacatcacg aacgacacca
     5521 tcggcaccgt gtggtacacg aagagaagta tcacgcactt cacgagactt gtctccaaag
     5581 atagcgtgca agagacgttc ttcagctgaa agatctttct cacccttagg tgttacttta
     5641 cctacaagaa tatcaccttc tttaacctca gcaccaatac ggataatccc catttcgtca
     5701 aggtctttga gggcatcttc accaacgttt ggaatttcgc gagtgatttc ttcaggccca
     5761 agctttgtat cgcgcgtttc tgattcgtat tcttcaaggt gaacagatgt gtagacatcg
     5821 tccttcacca agcgttcgct catgataacg gcatcctcga agttgtaacc ttcccaagtc
     5881 atgtaggcaa cgattgggtt ttgtccaagc gccatttctc cattttccat agaaggtccg
     5941 tcagcgatga aatcgccttt ttcaacgaca tcaccaactt ttacgagagt gcgttggttg
     6001 taagcagtac ctgagtttga acgacggaat ttttggatgt ggtaaacatc caatgatcca
     6061 tcttcacgac gaacttctac cttgtcagca tctgcgtaag taactttacc atcatactga
     6121 gcaatcacag ccgcaccaga atcgtgggct gcttggtatt ccataccagt accaacgtaa
     6181 ggtgcctgag gattaatcaa tggcacagcc tgacgttgca tattggctcc catgagggca
     6241 cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc aactacctgt
     6301 tttggtgaaa cgtccatgta gtcaacaata ttagctggat actcttggtt gaccccttgg
     6361 tgacgtccca tgacaatctt ctcagcaaag gttccatctt cattcagacg agagttagcc
     6421 tgagctacag tatattcatc ttcttcatca gctgtcaacc aaacaatttc gttcgtgaca
     6481 acacctgttt cacggtcaac cttacggtat ggtgtttgaa caaaaccata tttgttcaag
     6541 tgtccgtaag atgacaagtt attgatcaaa ccgatgttag gtccttcagg tgtctcgatt
     6601 ggacacatac gaccatagtg agtgtagtgc acgtcacgta cttcatatcc agcacggtca
     6661 cgagtcaaac caccaggtcc taaggctgac aaacggcgtt tgtgagacaa ctcagaaagc
     6721 gggttgtgtt ggtccatgaa ctgtgacaac tgtgatgaac caaagaattc tttaactgca
     6781 gctgttacag gacggatatt gataatttgt tgtggtgtca agacttcatt gtcctgaaca
     6841 gacatacgtt cacggacatt acgttccata cgagaaagtc ccaaacgtac ttggttggca
     6901 agcaattcac caaccgcacg gatacgacga tttccaaggt ggtcgatatc atctacacgg
     6961 ccaagtcctt cagccaagtt gaggaagtag ctcatctcag caaggatatc tgcaggagtc
     7021 accgtacgaa ccttgtcatc tgggttagca ttaccaatga tcgttacgac gcgatctgga
     7081 tcagttggag caacaacctt gaatttttga agaacaacag gctcagtcac aacggctgca
     7141 tcgtttggga tgtagacaat cttgttcaag tcgccatcca aatggctttc aatgctttca
     7201 atcacgctac gagtcataat cgtaccagct tctaccaaga tttctccagt ttcagggtct
     7261 accaatggct ctgcaatggt ttggttgagc aaacgtgttt taacattgag ttttttattg
     7321 attttgtaac gaccaactgc tgccaagtca tagcgacgtg ggtcaaagaa gcgagccaca
     7381 agcaagctac gtgagctttc agctgtctta ggctcacctg gacgaaggcg ttcgtaaatt
     7441 tctttcaagg cttcgtctgt acgagagtcc attggatttt tgtggatatc tttttcaaca
     7501 gtgttgcgaa ccaattcgct gtcaccaaag atatcaaaga tttcatcatc acctgagaaa
     7561 ccaagagcac gaaccaaggt tgtaaatgga atcttacgag tacggtcgat acgagtgtag
     7621 gtgatatctt ttgagtcgct ttcaagttcc aaccaagctc cacggttagg gataacagtt
     7681 gaaccatagc ccaccttacc atttttgtct actttgtcgt taaagtaaac acctggtgag
     7741 cggaccaact gagaaacgat aatacgttca ccaccattga tgatgaaagt acccatttct
     7801 gtcatgattg ggaaatcacc aaagaaaact tcttgggtct tgatttcgcc tgtttcttta
     7861 ttgatcaaac ggaaggttac aaaaattggt gctgagtagc tagcatcgtg gatacgagct
     7921 tcttctagcg tgtattttgg ttccttgatt tcatatccaa caaattccaa ctccattgtg
     7981 tctgtgaagt ttgaaattgg caatacatct tcaaacactt ccttaagacc gtggtctagg
     8041 aaagctttga atgagtcagt ttgaatttca atcaaatttg gtaagtcaag aacttctttg
     8101 attcttgaaa aactacgacg ggtacgatgt ttcccgtatt gaacgtcatg tcctgccaag
     8161 atgattctcc tttgtaaata agttccaagc cttgtcaatc aggcttttct aatcgtcata
     8221 tggttgtaaa ccccttatca ccgtgtcctt ttgacgaatt ttcagaatct ttaagcctct
     8281 gttacaaatg ctcaaaatct tgaaaaaaag cacaaaaaga gcagctaaat ctgacttttt
     8341 cagaagattt aactgctgtg agccttgtct ggacaatatt tcagacaaaa cctacgacaa
     8401 atgattaccc atattatacc ctatttagct agatttttca aggggtttca gtaggttttt
     8461 ggtaaatttt ttcccataga aaacttggca tcacattcga atcacgctat ggtacaaaaa
     8521 actgaaaaaa ctattgactg aaaatcattt tcaaggtata ataataaacg ttaaggcggt
     8581 atagccaagt ggtaaggcac ggctctgcaa aagcttgatc gtcggttcaa atccgtctac
     8641 cgccttctat aacttgattt atcaggtttc aaatgaacag aaagcccaat ttgaagggct
     8701 tttcttattt tccctcgaat aaatacgtat aactttaaaa acttttggag cgagtttgtg
     8761 gcagagttct ttccatggca taattccctt ttgaaatcaa ttgaaaaaag cttcgtcacg
     8821 cctggtgaca aaagcctcta gtttattctg tttcttcttc tatttcgact tctgtgattt
     8881 ggacttttac cttggtaaca cgtccattct tcactttatc attggttaag ataaactgtt
     8941 tgttttggct gaccaattca tagctgagtt tctcagtcgt tggaatagtt cccactcctg
     9001 tcaaataata accagcgatg gtatcaacgt catcactttc cagttcaaca tcaaagtaat
     9061 tattgaaatc attgagggtc atagttcctt gaacaatgta ggtatcctcg ccaatctgat
     9121 gaacatctat tgctgctttg tccgtttcat catcaatttc accaacaatt tcctctaaga
     9181 ggtcttccaa agtcaccaaa ccagccatac caccgtattc atccagcaag attgccattt
     9241 gtctttgggt atttcgcagt tcttttagca agtcatccac aaaaatagtt tcaggtacaa
     9301 aaagtggatc ttgtaaaatt ctcttccaaa caatattgtc aaaaccgtcc acaaagcctg
     9361 ccttaaggag actcttggtg tgaatgattc caattacatt gtccttatcc ccatcataaa
     9421 ccgggatacg agaataattt tgttttaaaa tactttggat aatggcttga ctatcatcct
     9481 gaatatccac cataaaggca tccgttcgag gcaccataac ctctcgtgcc atcagttcat
     9541 cgagcgaaaa gacaccttgt agcatctcaa tctcatcagc atccaatgtt tcttcactat
     9601 ttgtcagcat ataggcaatt tcatcacggg tcatcttttc atccgcatca tcgaatgaca
     9661 taggagtcaa atggctcaag aaattggtcg aagcagctaa aagccaaaca aaaggactga
     9721 ctagttttcc gatcccaatg ataatcggcg ctgtacgaat tgccaaggca tcctttagat
     9781 taagagcgat tctcttagga tataattccc caaaaacgat ggaaatatag gtcaaaaatg
     9841 ccaaggatag aaaagttgcc acggcttgtg ctgtttcgcc attcccaagc caagaggcaa
     9901 tcacacgtcc tagagtatca gttaaactcg cccctgataa gattgtaatc agggtgattc
     9961 ctacctgaat ggttgataaa aagtggttag gattttctag taccttcagc aggcggatgt
    10021 agcgtctgtc tccttcttcc gccttttgtt caactcgggc acgattaaga gaaaccatgg
    10081 ccatttctgt ggctgagaaa aaggcattta ataccgtcaa gataaacaat aaaacaaatt
    10141 gtagcaacaa attctgacta cttgggtctt ccatagttct tctcctaaaa tagtatctta
    10201 tcctttatta tatcacaaaa tttaatccaa tacatattat ttttttctta gcgtaacttc
    10261 catctctctc ccaaaactgg aagttagtct aagacggatt tccactgatt tcacgcgttt
    10321 ttctcatact aaaagctagg ttgtgggaaa ctggaagtta gttttagaag ttactatttt
    10381 ttcttagtac ctattctagc cctgcttaaa ctagtaaaaa gaactcctac atccataagt
    10441 gacataggag tttaggctta tcttttactg agttacagtt acttctccag ttcggtaatc
    10501 cagttgaagt cccttttgaa cattgggaac tagaacattg aattccaatt ctccatccga
    10561 agacttggct tcaatctgtg aagctgaggg aactaaatcc tcgtttgaag cgtagtaaag
    10621 ggttacacgg taacggacac gcttgttttg gtccaaggct ttacgcacct tgctttcata
    10681 gtagttttga ccagtcgaat actcggcttg tgcctgattt gcccaggctg tctgaacagc
    10741 aatgttttta ggattgcttg ttgaggcatc aaaaccatcc aaaccaccga ttaaggcata
    10801 gcctaacaaa tgacctctat cgactgcatg ggtataagag ccctttagat tcttgacctg
    10861 atgccaacct ggaggagtcc aagaagttga accattccca gtttctttac gattcttgta
    10921 ctgacgagtg gcattagaca agagggcatt agctacggtt ggaacagttt ccttgcccac
    10981 tgtctttgtt ttattgtcag cgtagggctt acttgaaacc ttggcatcta gatttgtttt
    11041 attaccattg acgataaaag cacctgagcc attccactcc agactcccct ttatttgact
    11101 cttgactgcg tctgttaaga cactctctgc caatgcttga ctaggagctt caggcgcttg
    11161 ttttttctga ctaagattgg ttttgggact attaggtgcc gactgcatct gcttgatata
    11221 atagctccct gtagacaaaa gcaataacac tagcagtccg attagtgtct gtcttgtttt
    11281 tttgttcata tttctcctta tctttagaaa aaggctggtt ctccagccta atttcctgta
    11341 aatttatgaa tcaattcctg ccattttgct ggagacagga tagcccatgg atcttgaccc
    11401 ttgcccaaga ttccataacc taccattaga ccgattccta gagccagagt acctaaaatc
    11461 agtactatga tgactaaaag taaacgcttg actacgtagc ttgatttctt attcatcttc
    11521 atcacttgcc tcaatccgct gaatcttagc acctagctga gccaacttct catggaaacc
    11581 gtagtaacct ctatccaagt gaaccaattt accgaccaca gtttctccct gtgctaccaa
    11641 acctgtcaaa atcaaggctg cactggcacg aaggtcagtt gaaagaactt ctgctccctg
    11701 caaaggctgt ccaccaacaa tacgagctgt atcacggata atctcagaat gcaagcccat
    11761 gcggcgcatc tcttctaggt gttggaaacg attttcgaaa actgtctcca ccatggttga
    11821 ttcgcctttt gcaactgtca tcaaggctgt aaattgagcc tgcatatctg ttggaaatcc
    11881 tgggtggggc aaggttttca catgaacagc ttttagattt tctagttgag aacgaacacg
    11941 aattccttcg tcttcttcaa ttacttcaac acccatttca agtaacttgg caatcaaggg
    12001 acggttgtgc tcccagacag cgtctcgaat caagacatca ccaccagtca tggcagcagc
    12061 taccataaag gttcctgctt cgatacggtc ctggactaca ttgtgagtcg taccatgaag
    12121 tttctcaaca ccagtaatgg ttatagtctc tgtaccagca cctttgacct tggctcccat
    12181 ttcattaagg agaatggcta agtcaacaat ctcaggctca cgcgcagcat tctcaatcac
    12241 tgtcacccca tcagccagag tcgctgccat catcaagttc tgcgttgcac caacacttgg
    12301 aaagtccata tagatatgag caccatgcaa gcgttctgcc tttgcttcga tgtaaccagc
    12361 tgtctgacta atcttaaccc ccatagcttc cagacctttc aaatgaagat caataggacg
    12421 gctaccaatc gtacaaccac ctggcatgga taccttggca tgacccacac gggcaaggat
    12481 tggccctaat acaacgatgg aggcgcgcat cttactgaca tacttgtaag gggcttcctc
    12541 agtgatgtcg ccagtagcat ccaccttgac aagatgagct tcctcatcaa agtcaacctt
    12601 ggcattcaaa ccaccaacta cctgattcat aataaagaca tccgacaaaa tcggaacatt
    12661 ctgcaagacg gtctttcctt cacttgctag aatagtcgct gccaacaagg gtaagactgc
    12721 attttttgct ccctcgatcg tcacgcttcc taccagacga ttatcgccac cttgaaccac
    12781 aattttatcc atactcgttt cctttactct tgattttata ataatcctct aagcgcttct
    12841 tgccacaaat gatacaggct aataaagaaa gaactcagga tgtagcctat gacaatgcta
    12901 aaaaaggcta ctaataaacg aacttttctt gtattctcat ctgtcacttt taaaaccttc
    12961 tcccatctaa caagatcctt taaaaggtaa aaactcacat aaataaagag catatgacta
    13021 cttagagtga ataataattg aatcatttga ctattatacc atcttctctg tttgtgcgct
    13081 agtttggaaa cttcactcaa aaattaggga ttgtttttca agtaatcaat cgcatcttgt
    13141 agggttttaa cgggcacgat tttcatatcc gtcttgattg ttttagccgc ttctagggct
    13201 gtttggtagt tgtttttcgc gtccggatgc gccttttgtt cttcttcgct aacagggtta
    13261 tcaggagtaa agaaaatagc agcacctgcc ctagccgaag ctacaacctt cttatcaata
    13321 cctccaatgt ctcccacatt accatcgcgg tcaatggtac ctgtaccggc aacaatacga
    13381 ccattacgaa gatctgggtg agctatttga gtatagatag ctagactaaa catgagacca
    13441 gcacttggac cgccaatacc agctgttgaa aagctaattg ggacattgct gattacctct
    13501 gtacggtcaa tcaagccgat tccaattcca tttttgccat tttccaaggt gatgattttt
    13561 ccttctgcag acttggtttg cccatcctct tcataggtga ccttgacgga atcccctaat
    13621 ttttgagaac tgacgtaatc aatcaagtct ttggaactat caaaggtctg atcattgact
    13681 gctgtgactg tatcagagat attgagaatc cctttaaagg ttgaattatc cgtcacattc
    13741 aaaacataaa ctccaaagta cttgagttcg atatccttac cagctgtttt tagtccttga
    13801 tacttggcca tattttgcga tgtttgcatg tagaattgat tgattcgcat aaattcaaca
    13861 tcggaagaac cacctgtagt ctcctgagca ctacgaatat ctgtaaaagg tgtcaaccaa
    13921 gcataaatca tatgagctaa agtggcatgt tgaacaccaa ccgtaacgaa ttgataggca
    13981 ccagcttcct tatcttctgt gtcatttact ttaaggactt ggcgaatatc ttccgaacca
    14041 cctggaacct ctatataata aggcaagggc actacaaatg ccaagaaagt cacaatcaag
    14101 gccgcaatga cgtataaggg ccatctaatc ttttttttca tttcttattt cctccaaaat
    14161 actctcggga acatagcagg caatatcctg accaaacttc aaaagctctc taacgcctga
    14221 tgaactgata tagagatgtt caggtcgact atgtaaataa atagtctcta tatcagaaga
    14281 cagctgatga ttgtagtaat caaaactggc ttcatattgc aaatccgacg catttctcaa
    14341 gcctcgcact agacaagtag cacccaatct ttttgcaaca tcgaccacca attcatcatg
    14401 agaagacacg actttaacat ttcccaaatg tttcacagcc ttttctaacc cccgtttacg
    14461 attttcaaga gggagaaatc cttgtttgtg gggattaaaa aaaataccca cataaagctt
    14521 atcaaagagt ctgctcgccc gttcaatcat atccagatgc ccatttgtca tcggatcaaa
    14581 tgagcctgtg aataagccaa tcttatctga catagactgt caccttacta attccataaa
    14641 tcttttcctt ccagataccc agacaggcaa tctcttctgg aagttcaacg gccttatccg
    14701 tctcgcacac aaccataaca tcttcagaaa aaagctctct ctcagccatt ttttcaatat
    14761 ctgctacgat ttgttccttg gcatagggag ggtctaagaa aacgaggtca aattccccag
    14821 atacctgttc caatgccctc tctgcatcca tcttgaggag ttgaaatttt ccaacttcct
    14881 tggtcatctg gatattttca gccacgatgg tctgagcctt acggtctcgc tccaccaaaa
    14941 cagcactgga catgccacgc gatactgctt cgatagataa accaccacta cctgcataaa
    15001 ggtccaagac tcgtccccct tcaaagtagg gaccaatcat gttaaaaatg gctcccctaa
    15061 ccttatccga agtaggtctt gtcgtcttgc cttctagtgt cttgagggga cgtcccccat
    15121 agattcctga tacgattttc atactgttta ttataccaaa ttatagacaa aaagagaaag
    15181 aaaaccgaac cttgcggttc gattctctac aaaatatttt cgtaagtatc gcggacttct
    15241 tgaggccaaa cacttgtttg cacttctccg atgtgtctct tgcgaagtag gaacatggcc
    15301 atacgagatt gtccaattcc tccaccgatt gtcaatggga atagaccatt caacaaagac
    15361 ttgtgccatt ccaattccaa gcggtcttca tcacctgtaa tttccacctg acgtctaaga
    15421 gtttcttcat ctacacgaat tcccatagaa gacaactcaa aggctccacc taaagactca
    15481 ttccagacaa gaatatcacc atttagaccc ttgtagccat tctcagactc gcttgtccag
    15541 tcatcatagt ctggtgcacg tccatcgtgc ggtttaccat ctggcaactc gccaccgata
    15601 ccaatcaaaa agacggctcc aaattcttta caaatcgcat tttcacgttc tttcggtgtc
    15661 aagtctgggt agcgttctac caattcttct gtatggataa aggtgatttg ttttggcaag
    15721 atagactcga tgtcatagcg ggcttcaaca gctagctcag tcagacgaat agccttataa
    15781 atcttctcaa ctgtttcttt tagataaacg atgttacgct taccatttgg gataaccttc
    15841 tcccagtccc actggtcaac ataaacagag tgggttgcat ccaaggaatc ctcatctgga
    15901 cgaagggctt tcatgtggac aaagagaccc tctccttcac caaagccaaa acgagccaag
    15961 gtgtggcgtt tccatttagc aagtgagtgc accacttcat aagtagcatc agggatttgg
    16021 agaaccttga ccgatactgg attttccaca ccagacaggt tgtcctgcat tccgtcaccg
    16081 accttactca agataggacc ttgaacttcg acaacttcta gcttatcttt caaatactgg
    16141 gtaaaagtgt ttttgacaaa ggaaatttct tcttgttgat ggataaaact tttcttcata
    16201 gacaactcct caaaaattta ataaacgtat tatacaccta tttccaagaa aagaaaagag
    16261 aaaatttaaa aaaaatcagt gtcactcaaa acactgattt catagacctt ttagtcattg
    16321 cgaccaaaga tacgtagaat acttaggaaa agattgataa aatcaagata gatgctgaga
    16381 gccattgaca ccacccagcc tgtcgctaca cgaccttgtg attgttcata ggcgaggcga
    16441 atcttttggt tatcccaagc aatcaaccct gaaaaaacca aaaccatggc tacgctaatc
    16501 atataatcaa agaagccact agccaagaaa atattgacta ccatagcaat cagtaaacca
    16561 atgagagctg ccatcattgc tcgaccaatc ccactcaagt ctttcttggt aaaaatacca
    16621 actgccgcca tgacaaagaa gagaagggcg ctcgatacaa aggcagataa aactgtaccc
    16681 ggagtataga aagccaccac aaaactaagg gtaaagccgt ttaaaacgga gtaaagtaaa
    16741 aatactggaa gagcagctgg actatttctt gaagccatgc tactggcaac gaaaaccaaa
    16801 gccaactccg caaaagtagc aatggtcaac cagagacgcc cctgcatcaa aaagtaaacc
    16861 aactgagact gaaagaccgt caacataagg cctgatacca aagcagatag tccgattccc
    16921 agaccaacaa aggcataaac cttagcgtaa aattgattga gacctgcacg gtcatgtata
    16981 atagtgtgat tcatccattt ctccttttct gtgaacatct ttccttgatt ataacaaaaa
    17041 aagttaaaat tagcttaaat ggaagattaa aataccggct gcaaccgcaa cattcaaact
    17101 ctcggcctgc cccttcatgc taatatggac caactggtct gcactttcag ccataagggg
    17161 actaattcct tgaccctcat ttcccataac tagtacaaaa ttttctatag aaggcagttc
    17221 tctgtaatca acagaatctt tagatagggt tgttgctagc actgggatag ctaccttttt
    17281 agtttcgtca agaagcgctt gactagtcat ccggtaaatg ggcagatgga aatgactacc
    17341 ttgcatggaa cgtagggttt tgagactgta gatgtctgcc gacttatctg aaacaatcac
    17401 tcctgtaaaa cctgctgcat ccgcagttcg aatgatagtt cctacattac caggatcttg
    17461 cacatcttcc aaaaacaaga acttgccctg actcaaatca gcttgtccta cttcttcttt
    17521 ttgaaccacg gcaacaattc cctgtggagt ctgagaatcc gccaaatcta gcaaaatatc
    17581 ctctgacacc cagacagttt gcgaaaaagt agctaactga tctcgataat tttctagggc
    17641 aaagattttc tcaatcgtca ctccagcttg gacagcttct tcaaacaagt gccagccttc
    17701 aatcaagtag gcagacttgc ggtatttttt ttggcgtaat ttcttggcat tttttaccac
    17761 agaattggct tttgaggtta taatagtcat agaaatatta taacacaatc aaaggggttt
    17821 ggtatgcaaa aggttagaat gattgcccaa ggtagggtac agggagtcgg ctttcgttgg
    17881 ggtgtttaca gcttggcact tgaaattggt ggcatcacag gtcgagtatg gaataacgac
    17941 gatggcacag tggaaatctt agcccaagca gactcatctg ctatcatggc aaaatttatc
    18001 caagaaatcc gaaaaggacc gacacctttt tcaaaagtaa gctacttaga tgtcaaacta
    18061 agcaactttc ctccctactc tgactttaaa atcgcaaatt aggtctccag aactattgta
    18121 tattttgtaa aaaaacagta gaatagaaag gtataatttt taaagaagga ataaaaacag
    18181 tgaaatctat taaacgtttt gcactctcag ctatgggagc ggctatgttg cttgtcttga
    18241 ctggttgtgt caatgtcgat aaaaccacag gtcagccaac aggatttatt tggaatacca
    18301 tcggagcccc tatggctgaa gctatcaagt acttcgctac tgataaaggt ctaggctttg
    18361 gtgtcgctat catcatcgta accattatcg tgcgcttgat tatcttgcca cttggtatct
    18421 accaatcatg gaaggcaacg cttcactctg aaaagatgaa cgccctcaag cacgtccttg
    18481 agccacacca aacgcgtctc aaagaagcga ctactcaaga agaaaaactc gaagcccaac
    18541 aagctctctt tgctgctcaa aaagagcacg gtatcagcat gtttggcggt gtaggatgtt
    18601 tccctatcct ccttcaaatg cctttcttct ctgctatcta ctttgctgcc caacatactg
    18661 aaggggttgc tcaagcaagc tacctaggca ttcctctagg ttctccaagt atgattttgg
    18721 ttgcctgtgc tggtgtcctt tactatcttc aatcgctcct ttcacttcac ggagtagaag
    18781 atgaaatgca aagagaacaa atcaagaaaa tgatttacat gagcccactc atgatcgtcg
    18841 tcttctccct cttctcacca gctagtgtca cactttactg ggttgtcggt ggtttcatga
    18901 tgattctcca acagtttatc gtcaactata tcgttcgtcc aaaacttcgc aaaaaagtcc
    18961 gtgaagaact agccaagaac ccaccaaaag caagtgcttt ctctaaacca agtggacgaa
    19021 aagacgttac ccctgaacaa ccaactgcta tcacaagcaa gaaaaaacac aaaaatcgta
    19081 acgctggaaa acaacgttcg agataagaag aaaaaggctt agctgatttg ctaagtcttt
    19141 ttgcaaatca aaagcccgat gtttccatca ggcttctttt ctatccatgt acacgtttca
    19201 tataatcttg ataactttcg gtatccatga gttttttagc gttcttgacg cgatctgctg
    19261 ttggtggttt gactccttcg agggaatatg gaattccaag ttcacgccac ttgaactcac
    19321 ccatggtgtg ataaggtaga atttcaaact tatcaacatt tttgagggtc ttgacgaact
    19381 taccaagttc aatcaagtca tcatctctgt ctgtcaatcc tggaactagc acgtggcgaa
    19441 tccagacagg ttttccaata tctgatagat actgggcaca agccaagata tttttattag
    19501 tttggctagt gacaatcttg tgctgttctt cgttgatttc cttgatatcc aaaagaacca
    19561 agtcagtgac agccatgagt ttgtcaaact tctcaaggta acgtggttta ttacggaaag
    19621 gaagagcaca ggtatccaag gtacagtgga ttccttgttc cttagccttg gtgaagagag
    19681 caatcaggaa atcaatctgc aagagagctt ctcctccact gactgtaatc ccacccttat
    19741 ttccccagaa accacggtag cgcaaggcct ctgtcaagac atcatctacc gtccgttcac
    19801 gtgacttatt ggactccata gcccaagtgt ctgggttgtg gcaatactgg caacgcatgt
    19861 gacagccctg caaaaagaca ataaagcgaa taccaggccc atctactgac ccaaagcttt
    19921 ctgtcgaatg caccattcct gtcacttgtc cataatcaat tgtttcttca gacatatcgt
    19981 taccttcctt gaaaacgttt tatagtttta ttatatcacg acttgaagga aaaacaagat
    20041 ttttgcctat tttttagaaa gcaatctgtt tttcaatttc attttcaatc tattatcaga
    20101 ttattcttca aaatcgaagc catataaggt tgcaaaatag tcctcagggc gctctgcacg
    20161 gcggatttga cgggctttac cttcttcggt atagaggatt tccgcagaac gtaatttggc
    20221 attatactgg tagcccattg aaaatccgtg ggcacctgta tcatgaatta ccagcaaatc
    20281 accgatttct gtatgaggca gttcgcgatt gactgcaaat ttatcattgt tttcacagag
    20341 tgaaccaacc acatctaccg cttcagctgg tccatctgga tgggtcacgt tgctaatatg
    20401 atggtaagct ccgtacatag ctggacgcat gaggttgact gctgaggcat ccacacctag
    20461 ataggtacgg taggtttcct tcttatgagt cactcttgtg actagagcac cgtgaggtgc
    20521 cagcataaaa cgacccaatt cggtgaaaat cttgacctga ccaagacctg ctgacgtaag
    20581 aacttcttca tacaccttac gaactccctc accaatcaag gcgatatcgt tcggctcctg
    20641 gtctggacga taattaacac caataccgcc agaaagattg ataaagtcta acgaaatgcc
    20701 caacttttcc ttgatttcaa cagccagttc aaagagctga cgagccaact ctggataata
    20761 gagatgggtc acggtattgg acgctaggaa ggagtgaagc ccaaaagtct tggctccttt
    20821 ttccttcaag atagcaaagg cttcaaagag ctggtccttg gtcatgccaa acttagcctc
    20881 ctcaggattg tccataatgt ctgtccccag ttcaaaaacg cctccaggat tataacgaca
    20941 agagatgatt tctggaatgc ctgctactct ctccagatgt tcaatatctt caaaggcatc
    21001 caagttaatg gtcgcaccca attcacgtgc ataggcgtat tccttgtctg gcgtgttgtt
    21061 ggaagagaac ataatctcag aacccagaaa gtccagttta tggctcatca aaagctctac
    21121 ataactagag cagtccacac cacaaccttc ttcttggaga attttcaaaa tagctggagt
    21181 tggagtagcc ttaactgcaa aatattcctt aaagcccttg ttccacgaaa aagcttggtt
    21241 gacagctctt gccttctcac gaatcccctt ctcatcatac aagtgaaagg gagtcgggaa
    21301 ctcggcaaca atcgcttcta actcttctct attgataaat ggtgttttca taagcttcct
    21361 tctattctgt ttgcaaagaa aggctgggac aatcgttcca acctttagtt ctatctctta
    21421 tttatcttcg tcaaagatat tgccaacctc aacatcgatg gcttggttct tgacatcaat
    21481 attagttacc ttcaagagtg acttgtagtc aaactgcttt tcacgttctt cttgggcatt
    21541 gtccgcaaag accgctacac ctgccagttc tgagtcgaac tcgcgcaaga gactaatcat
    21601 accgttgacc gttccgccac ctttcaagaa gtcatccaca atcaagacac ggctgcctgc
    21661 cttaagacta cgttttgaaa ggaacatttt ctcgatacgg tcaccacttg aacctgaaac
    21721 atagttgacg ctaacagttg aaccttcggt aattttcagg tcacggcgca caatgacaaa
    21781 agagacattg aggacattgg caactgcatt tgcaagtggc acacccttag ttgctacggt
    21841 cataaccgcg tcaatttttt ggtccataaa gcttttggca ataatacgac caatattttt
    21901 caagatggct ggtgtgctaa gcaaatcaga cagatagata taaccacctg gcaagatacg
    21961 gtcactttct gacaacttgg tacgcaagtc ttcaaccatt tccttagcat cctgactcga
    22021 gatagacggt gtgaagatga cacctccgcc agccccagtc actgtctgga tatgaccgat
    22081 ttcaatttcc tcaaaggcgc gtttgataat gacgatatct tctgagatgg atgatttagc
    22141 agactcatac ttttcagcaa aagtattgag actagttagt ttataaggat tattaatcaa
    22201 atagttggaa atgacaacca tccgatcact tcttcttaat ttcatttcta tttcctattc
    22261 catttttatt caaaaaatca aaaagcaaac tagaaagctg gtcgctggtg gttcaaaaca
    22321 ctgctttgag attgtcgata gaactgacaa accctgtaat atacctgcat atatacatac
    22381 gacaaggcga tactacccta gtttgaagag attttcgaag agtattcatt tttgtctttt
    22441 acttattata ccatattcac ataaaaaaac gaacattctt atcctaaaaa atgctcattt
    22501 ttcttaaatt attaatctaa atctggttta tagaaggaac gattatccat agcgaagatt
    22561 ttattggaca tctctccttt atccaccaaa gccagagctg ttgacatcat catcatgctt
    22621 gcatccagat tgtcaatcat atggataatc tctgcctcca taatacgtgg acggactgga
    22681 cttccatatt caagcaagcc gtggtgactg aggatgacat gacgaagcaa aacgacttct
    22741 tccttggtat catcgatgcc gagttccata actgtcttgg taatttcgct atcaatgaga
    22801 gcgatatgtc caagaagatt gcctctgacc gtgtactcgg tctggtctgg tcccgtcaac
    22861 tcgatgacct tagccaagtc gtgcaacata atccccgcat agagcagact cttattgagc
    22921 tgaggataaa cttcgctaat agcgtctgcc aaacgtacca tggtcgccgt atgataagcc
    22981 aaccccgttt caaaggcatg gtggttggtc ttggcagctg gataggagta gaattcctta
    23041 tcatacttgg tgtagagatt tcggacaatc cgttgccaga caggattttc aattttgaaa
    23101 atcatttgcg acatgtagtc acgaatttcc ttgacatcaa ctggtgactt gaccttgaaa
    23161 tcggctgggt cattgggttc accagcttga ggcaggcgga gagtaatttg attgacttga
    23221 ggggtattgt tataaacttc tcggcgtcct ttcatgtgga caaccttacc tgcggtaaag
    23281 gcctcaatgt tatgaggttg ggcatcccag agcttcccat caatctcgcc actatcatct
    23341 tggaaggtaa aggctaggta gtttttccca gctcgagttt gcctcaggtc agctgatttg
    23401 attaggtaaa agccttcaaa tagctcatct tttttcatgt gactaatctt catattcttc
    23461 ctcattttct tgaaaatgga gtagatcaag cgcaggctcg ccttctgaca actcaatgtg
    23521 acggagcgtc cgctcgatag ctatggtacg acggtttaat agttcatcaa tattgccaga
    23581 ggcatgttgg agatgttttt gtgccttaac cagaatgcca ccaaacttgc caaactcggt
    23641 cttgacactg gcaagagtct tgctgatatg gtcggcactc ttttggatat taagggtctt
    23701 gaaaccaact gatagggagt taagaagggc tgatagggta cttggtcctg caacgataat
    23761 ctgttcttcc cgtctcaaat tatcaaagaa gaccggattg cggacgattt ctgagtagag
    23821 accttctgtc ggaacaaaca aaactccaaa attggtcgtc cgaggtggtg ctatgtactt
    23881 gttcctaata tccctagcaa agcgcttgac gcttgctagg agtgacttac gacagcgttc
    23941 aatctcatcc ttgtcacctg tctcataggc ttcttccaag cggtaataat ctgccagtgg
    24001 aaacttagag tcaattggca gatagacgta ttcttggtcg ccttgtccgg gtaacttgat
    24061 ggcatactcc actcgttcac tagagttttc aaccgttgcg tattctcgtt cgtactgggc
    24121 aggtgtcatg atgtcttcaa taatttgccc cagttgcaat tctcccagaa tccctcgcgt
    24181 cttggttcca gagagaacct tgttaagagc tccgacatca cgggcaactg tctgcatttc
    24241 tccaaggcca cgattgacag actccagttg tttagaaact gtctcaaagg aagcctgtaa
    24301 gcgtgtctgc aaggtctttt ctagtttttc ctcgaccgtc tggcgcattt gttccaaacg
    24361 ttgctcattt gattcctgca aggcttggag acgttggtct gtcttgtctc tagtttggag
    24421 gagattatct gtcatttctt gacggacttg ggtcagacct tggtgcaatt caatccgcac
    24481 ttcttgcaaa cggtcgctga caaccacttc caaatctttt tggtctaact ggctggcttg
    24541 tctggcttgg tcaaagcggt aatccaactg gtctgacaaa tgatctgcct gatcctccaa
    24601 gctcttactt aagtgtttct cctgcctatc ctgcctttgc caaatcagaa agagaccagc
    24661 taggttggca attaataata gaataagtaa actttccatc ctacctcctg tccttgctat
    24721 gcagtacgac cacataacca tctgggcaag tcaccgacac ttccctatct atatattcgt
    24781 tagaagcgta cactttttta aagaaaaaat tctcctctgt caactcatac ttagctccaa
    24841 gaatagtcag ctggctatcc cgaactggca taaaggctag atagtcgtag tctgaacgag
    24901 gttctagctg actggtccct tctgaacaat aggcaatcaa attttgccca tcctcaattt
    24961 ctatttgacg catatagggt gccaacttag gattgctagg tagaaagaca ttggccaaca
    25021 tatggtcaat acggccaccc aaggcaccga aaatagtgac ctgagcctga ggattttgtt
    25081 caaagatggt taagagagcc aattccagat ctgtatcatc cttttctggt cgggcttgga
    25141 caaaatactg ggcacgtttt tgaatcacct gtcgctcttc tgcagtcaca gaatcaaaat
    25201 ctccgactgc tagagcaaga ggtaagtctt cttccaagac ccagagcgag cctcgatcca
    25261 ccccaacaaa agcatcaaaa tctgtccgat aatgaccgcg gtttccgcct gcaaaaactg
    25321 caaccctagt ccagttgttt tctgagagtt tgtactcgct cattgacttc tcccttaaag
    25381 acataggaac ctgctacaaa aacagtcgca ccagcttctt tggcttgagc aatagtttgg
    25441 tcatcaatcc caccatccac ttcgatttca aagtttaaac ctttttcctg acgaagagca
    25501 accaactcac gaaccttatc catggtttct ggcagaaagg cttgcccacc aaaacctgga
    25561 ttaaccgtca tgactaaaac ttggtcaact agatgaagga cgtgcttgat ggcttcaact
    25621 ggtgtgccag gattgataac gactgaaggc ttaactccga gtgaacgaat tttttggagg
    25681 gcgccatgaa tatgaggcgt tgcttctaca tggatactga tgatgtctgc acctgcacgc
    25741 gcaaaatctt ccagatgatg ctcagggttt gacaccatca agtggcaatc gaaaaccatc
    25801 ttactatgag gacgaaggct ctcgaccaca cctgcaccaa aactgatttg cggtacaaaa
    25861 tgactgtcca tgatatcgat atgggcatat tctgccccag ttgcttctag acgtttgatt
    25921 tcacgttcaa agttggcata atctgctgcc agaattgacg gagcaatctt gtattgagac
    25981 ataggtttct ccttattttg gaattttttt gctgactttt ttataggttt ctctacgatt
    26041 ttcaatttcg ctgaggaatt gcaggtagtt gtcaaaacgg aaggtggcaa tggtcccctc
    26101 ttctacagct ggcttgacgg cacaagacgg ctcatgggta tgggtacaag tacgaaactt
    26161 acaatctcgg ctgacagtag caatctctgg aaaggcctga ttgaggtctt cagcccttga
    26221 tacttcatag tccagagatg aaaagcctgg tgtgtctgcg attttacccc cattgagatt
    26281 gtaaaaacta acagctcgag tagtatggcg cccacgaccc agactgtctg agatttctcc
    26341 tgtttcaaga ttgagatctg gtgcgatttt gttgagaaga gttgacttcc caacacctgt
    26401 ctgccccata aaaaccgtaa ccttgcctgt taacaaaggc aggagttcct ctttactggt
    26461 cacaaagtca tagccgatgt caccataggt ctgctggtaa aaatccagtt ctcccctatc
    26521 ttccaacaaa tccattttgg aaatatagac aatgggatgg atgcccttgt gctccaaaag
    26581 aaccaagaaa cgatccagca aattgctgtt aaaatcaggt tccttgacgg acatgattac
    26641 tacagcttga tcgatattga caataggcgg acgaaccaga ctgtttttcc gttcgtgaat
    26701 tttgaggata tagccttctg aattttcctc ggcagagaaa tctacccagt ccccaacata
    26761 aggggtatgg ccttttttac ggaaattccc acgcgcgcgt gtttgataaa cctggccatc
    26821 actctccaca tagtagaaac ctgccaaggc tttaatgatt tgtccctgca tcttagagtc
    26881 cttgcccttt aagtgcgtct gctagaccgg caaattctgc caagctgaga gcttccccac
    26941 gcacacttgg tgacaagcct gcctggtcca aagccttggt cagcttgtcc ttgacctctt
    27001 cagtcttacc aaagtaacct gtcaagttat tccacaaggt cttgcggcga tgggtaaaac
    27061 tagccttgga aaccttaaag aaaaagttct catcttctac tgctacggct ggctctggac
    27121 gacgcaccat tttcaagatg gctgaatcca catttggcgc tggcacaaag accgtacgag
    27181 gcacgataaa ggcaaccttg gctgtcatgt aatactgcac ggcgatagac aagctaccgt
    27241 aagccttggt gttaggctgg gctgaaatgc ggtccgctac ttctttctgc atcatgacca
    27301 caaactcaca aaaaggaatg ccactctcaa tcaagtgcat gagaataggc gtcgtgatgt
    27361 agtaaggcaa attagccact accttgattg gcaggtcagg atttttaaaa ttctggatat
    27421 gttgcgccaa atcaaccttg agaatatctt cgttaactac ggtcacatta tcaaaatcac
    27481 gcagagtatc tgccaaaatt ggcaccaaac ggtggtcaat ctcaaaagcc atgacttggg
    27541 ctgcacgctc agccaaaaat tctgtcaagg caccaatacc tggcccgatt tcgatgacat
    27601 tgacctgatc atcaatttca gccgtatcca caattttttg aaggatattg gtatccgtca
    27661 agaaattttg cccaaaggac tttttaaagg taaaaccgtg acgctccaac actgccttgg
    27721 tcacgctata atctgcaatt ctcatctatt ctcttttcta ttatctgtta ctactattgt
    27781 aacacattcc ccttgtattt tgggaatgaa aatcatacca aaaaactatc tttcctcatg
    27841 aagaaagata gtcttggaaa tctttaaaga gggacttagc gcttctctcg aaacatacgt
    27901 tcatactctt cgaaaatctc ttcaaatcac gtcagcttca ccttgccgta ctcaagtaca
    27961 gcctgcggct agcttcctag tttgctcttt gattttcatt gagtatcaaa aaaatcagct
    28021 tcttgataac ttcgattttc gggtagggtc aaatccacac cctcctccac atcatccact
    28081 gtcagttcaa ccgtttgagg taaatttttc aaacgataat gcttttgcct ttacccttca
    28141 atactagcaa aatcaagcaa atagccatgc actcggattt gaaccaaaag gagatgattg
    28201 gtcatatttt ccatgatgag gatatcttct aaaccacctc tggcactagg ataatcagat
    28261 accctgcgaa tacggatttt ttcacacatg agcaagtttc ctcatatcaa tgatttcatc
    28321 agccttattc cagactaggg gattatgggt cgcaatgatg ataattcgat tttcatcttt
    28381 caaatccacc aagagattca taacctcctc tgaattttca ggatcaagag ctgctgttgg
    28441 ttcatctgcc aaaatcaagg gtggattttt caaaatagtc ttagcaaggg caactcgttg
    28501 ggcctctccc ccagataaag tatagatttt ttgttctaaa tccaaatacc ctagattaac
    28561 tttttctaaa gcccccactt gcctttccaa acgttctact tttgagattt tctgaccaac
    28621 aaaacccaaa tccaaatttt ctttgattga ttggttttct aagaggccaa aattttgaaa
    28681 gagatagccc atctgatctc gaaaatactc acgagtggga atggttttta aatctttccc
    28741 ctgatagaga acccttcctc catctatctt ttccagcttt cccaagatat tcagcagcgt
    28801 cgtctttccg cttccactct ttccgattaa ggcataaacc ttgcccttct ccagcttgag
    28861 attcaaacca gagaaaatcg cgcggtcatt aaatttcttt tccaatcctt gaatatcaat
    28921 catcttattt tcctttcaat actgccatgc taccagcttc ttcttttttg tcctgcctta
    28981 ctaaaatcaa gagggcgtta agcgtaaaca aagctacaac tagagcgcta atcaaaccat
    29041 ctcttgtcaa aatactagat aggactaggc caagcaagag aactcctcct tgcgccagta
    29101 aatacttgcc atgaagctca tagattgtca taccagcaag acgtttaatc ataagctccc
    29161 gtctgaactg ctcaaaatag agaagattca tggaatcaaa taacaagata gccgtagaca
    29221 gggtcaaaat cgtcccaata aggagagagt aaaactccac ccgtttgtca tttagtacct
    29281 tggcaaaaaa tagctggctt tttaccaagt aagaaacctt gtgatacaga ccttgctctt
    29341 ttagagctgt gatgctgtct ccatatcgat ctagtttcaa ggcactatta gccgtagttc
    29401 cccacaacat ccctgcaaca ggtcttgttc cagtcgcttg cggcgttaga actacaataa
    29461 tcggatcttt taggtactgg cgtgttgtct tgtacccatc attatagagg aaacgttcct
    29521 gtcctgtttc tgtaaaagct agccttacct gtgggaggta gtgtttctga ctcgtcactt
    29581 ctacactttc agatgaaaag ttttgcaggt aatctgtaaa caatccttgg tagtaggcag
    29641 actgctctcg caagctctca ggcaagatca gcccaaactc tccctcagac aagttttgca
    29701 tcttgtccat aaactctgaa gaaacggtaa tcttttcctc tatcagatag cgcggtgaga
    29761 catagataac attccctgac ggtgtgtagt cactgagacg attgccatct aggtccactt
    29821 ctactccatc tgagaaattg tcaacattgc tcataatata aaaagagtct gtattggcta
    29881 accgttcttc agtaaatgtc tgccactcac gattatcctt acgcattcct tcttcatcgg
    29941 caaatgcact agaccaacca aaggagagac ggtaacggtc tgaggactgg ctccatttat
    30001 cgcttgcctt ctccatctcc tgcatttcac ggtagttggg gaggagagct gtcgcactca
    30061 agccgaccac caatacagct aagagttgcc ccaccatcat taatgtcatc atacgtttga
    30121 gagggagttt ccctttcaat agatccacca gactattttc ctgtaaacca agtagataga
    30181 cgacacttag taaggtagaa atccctgcca gggtcaatcc ataaagtaga agagcaatga
    30241 tgaccagttg caccgttgcc ataaacaagg caccttgata ccagagaatc cccaatccca
    30301 aaagactgga taccagcact gagcagataa actgtctcac atcttctaac actggtctga
    30361 gagcaactcc aaacaagctc tcaccagcta ttaagcgaat ccctgcctga cgaagggatt
    30421 tgatccgata aatcagggtc agactcataa aggtcagcag aaaaatagct aaactcagta
    30481 gcaccatagg ggtggccgcc aatagtaaga ctatcgaaaa tggatcttct ccattcgaaa
    30541 caaagccttg atagccaagt tctttcaagg tggtctgtaa gctcactcca tccaaacttc
    30601 ctgacacaat caagtaagac cctaataaat cactcgtttc tgcactctcc ttggaggaaa
    30661 tggtcagacc ttctggaagc tctccctcac cataagtggc ataggtaaag agtgtttctc
    30721 cagcttcatt tggctcaacg attcgacggg cgatgagact attatgctct accgccaagt
    30781 gactcaaaac ctctgtcacc tcttcgcgac tagctactgg ttgatttatc atcattcctg
    30841 tcacatcaac agaaggatag ttaccaaata aaatctcttc tcgatggtca acggaagtta
    30901 tcaccatata aagcgatacc aataccattg aaatcaaaat aaataaacgt ttcatcattt
    30961 cctccaaaaa gttacttggt cacttagtga gaaggtgacc acagtcactg cgatgttaag
    31021 aagcttatta agttctttct gctagcaaca tcttcaaata cattatatcc gttttttcaa
    31081 gaactatcaa ggacatcaat gtccttaaaa tctaaccatt tcgtgtcatt tttagcttat
    31141 atctaaaaaa cagccctaaa gacgaacttt agaactgttt tgatgcgaga acaaggacat
    31201 ttttgtcact gattcactat gtttataaat attttttgac atcctcttgc cattcctctg
    31261 tcaatttctt tactagaaat tcatctccaa ttagctgcgc aaatgtttta gaagactgat
    31321 aaaatgattc agctaaacta aaatctcttt cttctcttaa tgcttgcttc cacaaaaac
//
LOCUS       DAVHIU010000012        30418 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVHIU010000012 DAVHIU010000000
VERSION     DAVHIU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.012"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(146..235)
                     /locus_tag="VVN06_000441"
     CDS             complement(146..235)
                     /locus_tag="VVN06_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I addiction module toxin, Fst family"
                     /protein_id="HEU5562049.1"
                     /translation="MVDYIFTNIILPLVVGIILIVIEKWLNKK"
     gene            339..530
                     /locus_tag="VVN06_000442"
     CDS             339..530
                     /locus_tag="VVN06_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5562050.1"
                     /translation="MPDIANGREKVNAFLKDKGIKKTTLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            564..1100
                     /locus_tag="VVN06_000443"
     CDS             564..1100
                     /locus_tag="VVN06_000443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562051.1"
                     /translation="MEEFIDALEKEKDHLEKIIKVVSSGGKFLRLPYQKKSRSISENL
                     KLISQNLDRLSCLYNQRGERMTDRELFELPEDYVESTGLDKITFEVPFELFTKILKGY
                     GHKLAWEDYQQIKIHPSTRTKKTVGQCQFLFSIWMNDHLKPAIKPSKELQKEPVRKTK
                     RLKRLRKLAQNLLHRMKG"
     gene            1331..1603
                     /locus_tag="VVN06_000444"
     CDS             1331..1603
                     /locus_tag="VVN06_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562052.1"
                     /translation="MEAVEIVRIKDVIIEKVSANDEELEHIFGCSKRQAGDMRREMKK
                     LPSQQKHLRNDGQLVTIKGFDAYLQYRGSQSWKKEMVKSKKIRSVG"
     gene            1673..1999
                     /locus_tag="VVN06_000445"
     CDS             1673..1999
                     /locus_tag="VVN06_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562053.1"
                     /translation="MNEPSIVSQLLGNGAVILGFISAGILAHQLEKQKEEERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNIRRPFRGFTYDNEPPVGLRPEPLALPEPRRVQ
                     YANRMG"
     gene            1980..2171
                     /locus_tag="VVN06_000446"
     CDS             1980..2171
                     /locus_tag="VVN06_000446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562054.1"
                     /translation="MQIVWDRQAWDLSTCKRREKMRDLEMMAHMQHEIDDLKKQLQQE
                     QSLRKRLEAENFQLKLRRK"
     gene            2306..2647
                     /locus_tag="VVN06_000447"
     CDS             2306..2647
                     /locus_tag="VVN06_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562055.1"
                     /translation="MTQAERIRKYYKDHPTASYDEVAEVVGTSNSNVRVNLFKDLKAG
                     RCVRLEDESIDYSPYFNHKKSLTELFDWKNDTRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            2644..3120
                     /locus_tag="VVN06_000448"
     CDS             2644..3120
                     /locus_tag="VVN06_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEU5562056.1"
                     /translation="MTKSLYAIVGQFLEIYNMELDEETKLDTLDSIDWETDYETKVEN
                     CIKVVKNIDADMEARDAEIKRLTNLNKFDKKKKEYLKERVSESMKLTGHERVDTPLFK
                     VSFRKSQAVEVNEAVLPEVYKVATWKPDKKLLKEDLKNGLEIIGASLVERKNLSIR"
     gene            3117..3818
                     /locus_tag="VVN06_000449"
     CDS             3117..3818
                     /locus_tag="VVN06_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5562057.1"
                     /translation="MKITKATEITNDDACYLIYGNPGFGKTTTISFIPGKTLIINIDK
                     SAKVLAGNPNIDIADVDTHKIWDEWLTVVKELLQGAGKPYDTIVVDNVSELFRACLAN
                     LGRDGKNHRVPTQADYQRVDFTILDSLRALLQLNKRIVFTAWETSDQWSDENGMIYNR
                     AMPDIRSKILNNFLGLTDVVARLVKKTTDDGEEVRGFILQPSASVYAKNRLDDRKGCK
                     VDELFAQRLPEGTDN"
     gene            3775..5184
                     /locus_tag="VVN06_000450"
     CDS             3775..5184
                     /locus_tag="VVN06_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5562058.1"
                     /translation="MSFSLRDYQKELIIDVIKSMKAGNRKIMVQSPPRSGKTVVMSYI
                     AKNATDKDKTVLFFSHRKEINEQVHEAFKRGGVNLDKVIIGTVGSIVRKLERLPQVDV
                     ILVDEAHHIKAKQYQTILNYFSNATQLFFTGTPIRLDGSGFHDLADDLVVGKSILWLQ
                     DHGNIAEFDYYSINLLDMAKLKKRSGEFTNHSIDVAFDSKATYGDYIDHYERLAKGKQ
                     AIVYTHSVEYAERVAKRFLEQGYQSAVVSGKTPQGERESHMQAFREGKLTIMVNVNLF
                     TEGIDLPNVDVCIMLRPTDSLSLYLQFAMRALNPREGKKAILIDHVGNHIRHGLPNDD
                     REWTLDGTKKKKKSSERSTVTCEKCFATFWREQLVDGCCPYCKAEIVEKKNIQDIKQD
                     KSDIQLTKINQGMEFITIRGKEIEVKTEEAKVYRRVKTYGKRYTRCQNLSELKAFRLL
                     NGYQPGWLWHKQKELNLWR"
     gene            5190..5672
                     /locus_tag="VVN06_000451"
     CDS             5190..5672
                     /locus_tag="VVN06_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEU5562059.1"
                     /translation="MALFSVNYEAAEQFSSIEDGTYEVVVAQAEQSASQSGTDFLDIR
                     LKIRDDFQQKFRNNLIFDKVWINKQTLQYPEWALQRYSKAVKIPEGVEVNTIEQFLGL
                     IIGKTLKVTVKNEQSEYNGKTYDNLNIKKMEQSELPPYSGAVSSEPAPAKADDLDLPF
                     "
     gene            5676..6473
                     /locus_tag="VVN06_000452"
     CDS             5676..6473
                     /locus_tag="VVN06_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEU5562060.1"
                     /translation="MVGMVDYALHYQKLGYSVIPIDKKSKRAITKFKDKTFSENEIRR
                     FWHEQPDANIAWRTTDFFVIDIDVSVTENGYESLKEWELSQYIPKTLTATTPSGGKHI
                     FLKKPKGIELSQDIRVKPGIDIKANKNNYVLVAPSNNAKGSYKWDKSTEQMAEAPAEI
                     ISILQTSKQPKELMNFTTDYSRGEFSSKTAKLFEQVVFGLGDKGGRNNALASFIGGLL
                     MRGVDVDATYLLAKIANHYTPDSLPADEVDRTFESMVRKEMDRRGGY"
     gene            6409..8013
                     /locus_tag="VVN06_000453"
     CDS             6409..8013
                     /locus_tag="VVN06_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU5562061.1"
                     /translation="MKWIERLKVWLERRWIEEVAIDLQKLKEEYRNNIIQHPAYIEKA
                     NDWREIRLACREYRKNWLENVKWEETQYGTKEENKKAPTRLTELAVAKGMEQILYIVN
                     LPNERVAIYDPDKGYYHKDPSFAYKVIRLLEPNFSEAKSKNVLFMLASTPRLNQHEGF
                     SCDFSIGEYKDPRRFILVKNGIYDKKEKLLRPFTHEFVAFSTIGTEYDHFAKSHEIDG
                     WDIDSWLLDLMSGDEELVQLIWQVISASLNGNYSYRKSIWFVGEGNDGKGTVQQLITN
                     LVGMRNVASLKLNQFSERFALSMIEGKTVIIGDDVQAGIYVDESSNFNSVVTGEPILV
                     EEKNKQPYTTVFKKTVIQSTNELPRFKNKTNGTYRRFAIIPFKKSFSSKEDNWAIKDD
                     YIYREEVLEYVLKKALEISFDRFIEPQASIEALENFKESNDTVKAFVSEWFDKFESTR
                     LPSRFLWWLYQEWCKDEGVTRLTKRKFETQLAKNIPENWVKKKIKPLGKFIPSVDVPK
                     HYSGFSWLNDESQILTSGYELVTVYR"
     gene            8265..8573
                     /locus_tag="VVN06_000454"
     CDS             8265..8573
                     /locus_tag="VVN06_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEU5562062.1"
                     /translation="MKSEQEVQNEIRVALSQAGYTVFRTNVGKVKTADGRWFDTGLPK
                     GHADLYGFRPDGQIFYVEVKKENGRVRPEQENFIETVRKRGAIAGVARSAKEALELVK
                     "
     gene            8674..8841
                     /locus_tag="VVN06_000455"
     CDS             8674..8841
                     /locus_tag="VVN06_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562063.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            8828..9016
                     /locus_tag="VVN06_000456"
     CDS             8828..9016
                     /locus_tag="VVN06_000456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562064.1"
                     /translation="MKYDKQAEIDGLKRTIEQTEARIVELSEPCVKSLAFSRSEERDL
                     LKKKVKNWKKRIKELEDE"
     gene            9009..9533
                     /locus_tag="VVN06_000457"
     CDS             9009..9533
                     /locus_tag="VVN06_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU5562065.1"
                     /translation="MNKQELIEKYKELENSSFDIAAIVVCQLVLKDLEQLDEPKPVKV
                     KQFVADWYEENKDSFEFNVWDWIAFRDEAKKSENREFNNWINNTRENPIQTLVNMHNF
                     GYEVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKQLEEAGLG
                     WVFDCEGIEIEEVE"
     gene            9533..9820
                     /locus_tag="VVN06_000458"
     CDS             9533..9820
                     /locus_tag="VVN06_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562066.1"
                     /translation="MTQTLEEGMKNQSKCIKVPREIRPFDIGYRIVNKYGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEGVE"
     gene            9820..10008
                     /locus_tag="VVN06_000459"
     CDS             9820..10008
                     /locus_tag="VVN06_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEU5562067.1"
                     /translation="MSCSENLKKEKELTAAISNFKIEDLQNDDKLSSLSLSNIKRQAR
                     DLYECLVWLQYNAEESGR"
     gene            10005..10343
                     /locus_tag="VVN06_000460"
     CDS             10005..10343
                     /locus_tag="VVN06_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562068.1"
                     /translation="MSYDLEILAKIESGDYICIAEPRYSSPTYNLGKMFRVAMDWDFD
                     QGTIYNVADIFENIKRGITELERQPEKYVQYEPANKWGTINDALYVLRSLRDCILEQD
                     IDTKYLYVRW"
     gene            10494..10808
                     /locus_tag="VVN06_000461"
     CDS             10494..10808
                     /locus_tag="VVN06_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562069.1"
                     /translation="MEELKKKVNAVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYSPSMAQVVIAVYLIYR
                     GN"
     gene            10810..11472
                     /locus_tag="VVN06_000462"
                     /pseudo
     CDS             10810..11472
                     /locus_tag="VVN06_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041154.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            11777..12169
                     /locus_tag="VVN06_000463"
     CDS             11777..12169
                     /locus_tag="VVN06_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562070.1"
                     /translation="MIPKFRVWHYELGRLMSVKCMFFQDSEIEEFELNDALMNDYITA
                     YPDEIELMQSTGLKDKNGKEVFIGDIVKCTRGCPHEVYLEKEYGGTYIGGMPAIYLKG
                     LLSGYAWTEDEEIIGNIYENPELLEVNE"
     gene            12244..12483
                     /locus_tag="VVN06_000464"
     CDS             12244..12483
                     /locus_tag="VVN06_000464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5562071.1"
                     /translation="MVDKYIDEPHTTFIPVINGKSSVLVPTRTKRKYILVVSGYAGNK
                     QVEERFEVTANEYKHYEIGNTFTKDAVLENKEGDK"
     gene            12483..12854
                     /locus_tag="VVN06_000465"
     CDS             12483..12854
                     /locus_tag="VVN06_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562072.1"
                     /translation="MRPKKYPYSGRRKKQDTSSPLFSTRPIFNEIPIVEEVKVDLGVE
                     ANVGRSYPEMLIHLDISGYGNRIHSVHRFPGIFLTVGESIQLKMLFYKRLKNFTADRF
                     LTFRESDWKFFIRGLVNEFVH"
     gene            12924..13343
                     /locus_tag="VVN06_000466"
     CDS             12924..13343
                     /locus_tag="VVN06_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU5562073.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSDVKVK
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKSRSVLRMTYITK
                     TYIEDICDNLRISKATYYRLRKQAESELEETIIDKVS"
     gene            13425..13515
                     /locus_tag="VVN06_000467"
     tRNA            13425..13515
                     /locus_tag="VVN06_000467"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13461..13463,aa:Ser,seq:gct)
     gene            13761..13833
                     /locus_tag="VVN06_000468"
     tRNA            13761..13833
                     /locus_tag="VVN06_000468"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13794..13796,aa:Lys,seq:ttt)
     gene            13901..14341
                     /locus_tag="VVN06_000469"
     CDS             13901..14341
                     /locus_tag="VVN06_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU5562074.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            14319..15617
                     /locus_tag="VVN06_000470"
     CDS             14319..15617
                     /locus_tag="VVN06_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU5562075.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYSWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAELRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            15629..17098
                     /locus_tag="VVN06_000471"
     CDS             15629..17098
                     /locus_tag="VVN06_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU5562076.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSHLKKLKSYYM
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            17007..18644
                     /locus_tag="VVN06_000472"
     CDS             17007..18644
                     /locus_tag="VVN06_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU5562077.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEI
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPENEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKITTDSQAENISLSKATSKINDALRYTTIFDPDTFAKEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            18646..18855
                     /locus_tag="VVN06_000473"
     CDS             18646..18855
                     /locus_tag="VVN06_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562078.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            18852..19265
                     /locus_tag="VVN06_000474"
     CDS             18852..19265
                     /locus_tag="VVN06_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5562079.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIHYLST"
     gene            19308..19553
                     /locus_tag="VVN06_000475"
     CDS             19308..19553
                     /locus_tag="VVN06_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562080.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKLDIFAETYEKTEE"
     gene            19555..19806
                     /locus_tag="VVN06_000476"
     CDS             19555..19806
                     /locus_tag="VVN06_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562081.1"
                     /translation="MLEKAKQLASQEFSRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGNYAEITHNGDKKETYVDVYAKVSNRAIKD"
     gene            20023..20607
                     /locus_tag="VVN06_000477"
     CDS             20023..20607
                     /locus_tag="VVN06_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU5562082.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            20614..21459
                     /locus_tag="VVN06_000478"
     CDS             20614..21459
                     /locus_tag="VVN06_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEU5562083.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            21471..21653
                     /locus_tag="VVN06_000479"
     CDS             21471..21653
                     /locus_tag="VVN06_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEU5562084.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            21643..21831
                     /locus_tag="VVN06_000480"
     CDS             21643..21831
                     /locus_tag="VVN06_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562085.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            21833..22144
                     /locus_tag="VVN06_000481"
     CDS             21833..22144
                     /locus_tag="VVN06_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU5562086.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            22125..22463
                     /locus_tag="VVN06_000482"
     CDS             22125..22463
                     /locus_tag="VVN06_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562087.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            22438..22953
                     /locus_tag="VVN06_000483"
     CDS             22438..22953
                     /locus_tag="VVN06_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU5562088.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNAAIKVVQADAR
                     RLVPVNDGELITSIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSHPWYVHEDQINVGSYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIQ"
     gene            22950..23318
                     /locus_tag="VVN06_000484"
     CDS             22950..23318
                     /locus_tag="VVN06_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562089.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDKEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            23324..23737
                     /locus_tag="VVN06_000485"
     CDS             23324..23737
                     /locus_tag="VVN06_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU5562090.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYELGIGDVGELEYKFSYNNSSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFADGVGG"
     gene            23742..24122
                     /locus_tag="VVN06_000486"
     CDS             23742..24122
                     /locus_tag="VVN06_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562091.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKNKKTSKKKMTVVE"
     gene            24161..24511
                     /locus_tag="VVN06_000487"
     CDS             24161..24511
                     /locus_tag="VVN06_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562092.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKTQERKEKIIDSYR
                     LSQMISNHVSLLLSNDAKIVEFWEYAPELFVEEQQAVELERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            24498..28031
                     /locus_tag="VVN06_000488"
     CDS             24498..28031
                     /locus_tag="VVN06_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562093.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSAAMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIVTDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERIIRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAKFAHSVATGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTNALSSVSNWFGEMFTNAYNAVKDAFSSIGDFFKGVWDTVKSIFVNAGQMVGE
                     AVGGAFKSAVNAVLGTIENVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARG
                     GIVDSPTIAMIGEAGKEAVVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDI
                     VIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            28040..28390
                     /locus_tag="VVN06_000489"
     CDS             28040..28390
                     /locus_tag="VVN06_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562094.1"
                     /translation="MAQLTINGVAVKPPKYFQVGIQDIDGETGRNANGDMMRDRITTK
                     RKLDCEWGMMTQGELSQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            28395..30263
                     /locus_tag="VVN06_000490"
                     /pseudo
     CDS             28395..30263
                     /locus_tag="VVN06_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
BASE COUNT        10051 a         5150 c         7245 g         7972 t
ORIGIN      
        1 cttgttcctt gacaattgaa tagagcatgt gagataatag gggagaagtg aagatgtagt
       61 ctactataac tacaaaaaaa gcccctgctg ataaccacaa aagcaagggc tttttctagt
      121 ctacgctaga aaggtgggtt ggtcgctatt tcttgtttag ccatttttca atgactatca
      181 ggatgatacc gaccaccaag ggtagaataa tatttgtgaa gatgtaatct accataggct
      241 ccacctccct tctaaggcag atgtgccgtc actattatat cacatgctct atcagttaga
      301 tagggcattt tttatttttg gaaaaaggag gaacatatat gccagatatc gcaaacggtc
      361 gtgagaaagt caatgctttt ctgaaagaca agggtattaa aaaaacaact ctagcggttg
      421 cttatggctt taaacgacag gaagtaacga acatcttaag tgggacgacg aagggaccac
      481 gagcgaacag tttcattctt caagttattg aagattacgg gattgagtaa gaaagatttg
      541 aggaagctta gtaaggagag tctatggaag aatttattga tgctcttgaa aaagaaaaag
      601 atcaccttga aaaaatcatt aaggtagtca gctctggtgg taaatttctg agattaccat
      661 atcaaaaaaa gtcacgctcg attagtgaga atctgaaatt gatttctcaa aatcttgata
      721 gactgagctg tttatataac caaagaggag aaagaatgac agacagagaa ctgtttgagt
      781 taccagaaga ttatgtagaa tctactgggc ttgacaagat tacatttgaa gtaccttttg
      841 aattgttcac aaaaattcta aaagggtatg gacataaatt agcttgggag gattaccaac
      901 aaataaaaat ccacccaagc actagaacaa aaaagacggt tggacagtgt caatttttgt
      961 ttagtatttg gatgaatgac catttaaaac cagcgattaa gccttcaaaa gagttgcaga
     1021 aagaacctgt gcgtaagaca aagagactga aacgtcttcg taagttagca caaaatcttc
     1081 ttcatcgcat gaagggctaa aatcgccaat atatgcacct tgctctgttt tgcagattag
     1141 aagagcgcca tcttcatttg taactgcatt tagatagggt gccaaatcag acttagtcat
     1201 aagttatcct cctttctgct tacattatag cagaataaga agattggatt gcaaataaac
     1261 aaaaaagcac ctaacaaagt caggcgcata tcaaaataac taactgaatt ataacacgaa
     1321 aggagtaaaa atggaagcag ttgaaattgt aagaattaaa gatgtgatta ttgaaaaagt
     1381 ctctgctaat gatgaagaat tagaacacat ctttggatgc tcaaagcgac aagcgggaga
     1441 catgagacgc gagatgaaga agctacctag tcaacaaaaa cacctcagaa atgatggcca
     1501 gcttgtcaca atcaaaggtt ttgatgctta tctgcaatat cgaggcagtc agtcatggaa
     1561 gaaagaaatg gtgaaaagca agaaaataag gtcagtcgga tgaacttttt aacaaaaata
     1621 aaaaattggt tggaaaaaga gaaaaaatta aagatttaga ggagagagaa caatgaacga
     1681 accgtcaata gttagccagc ttttaggaaa tggcgcagtg attttagggt ttatcagcgc
     1741 aggtatttta gctcatcagt tagaaaagca aaaagaggaa gaaagacgag aagagcaaga
     1801 atttgcgtct atgattattc aagggtataa ccatgcttac gaacgtggta gagaggcaga
     1861 acgccaagaa atccgcaaga atatccgtcg tccgttcagg ggttttacat acgacaacga
     1921 accgcctgta ggcttgcgcc cagaacctct ggcattacca gagccacgga gagtacaata
     1981 tgcaaatcgt atgggataga caagcgtggg atttatccac ttgcaagcgt agagagaaga
     2041 tgcgtgacct tgaaatgatg gcgcatatgc aacatgaaat cgatgatctc aagaaacaat
     2101 tgcaacagga acaatcttta agaaagagat tagaagcaga gaatttccaa ctgaaactaa
     2161 ggaggaaatg atgtacttat ggaagtgtac gtgcgctgat tgtggacgtg agtttgattg
     2221 gtacgataac tatccacctc ttgaatgtgt gaaatgtgag agcgtggaaa tcaaaaatga
     2281 atttaaagga agggcgtatg attaaatgac tcaagcggaa cgaattagga aatattacaa
     2341 agaccaccct actgcctcat atgatgaagt ggctgaggtt gtcggaactt caaatagtaa
     2401 tgtgagggtt aatcttttta aagacctcaa ggcaggcaga tgtgtccgct tagaagatga
     2461 gtcaattgac tactcacctt attttaatca caaaaaatca ctgacagagt tgtttgattg
     2521 gaagaatgac actagacggg aatgggtaga tatgctgaca agagcagcag agaaagaaac
     2581 ggatagtaac gttatgcgtc tgctgatcaa ggaagcaaat aaattgatga aagaggtgac
     2641 gaaatgacga agagtttata tgcaatagta ggtcaatttc ttgaaatcta taacatggaa
     2701 ttagacgaag aaaccaaact agacaccttg gattccattg attgggaaac tgattatgag
     2761 actaaggttg aaaattgtat caaagttgtg aaaaatatcg atgcagatat ggaagcccgt
     2821 gacgcagaaa tcaaacgctt gacgaattta aacaagttcg acaagaagaa aaaagaatac
     2881 ttgaaagaac gagtttctga aagtatgaaa ttgacaggtc atgaacgtgt tgatacacca
     2941 ctttttaagg tatcatttag aaaatctcaa gcagttgaag tgaatgaggc ggtcttgcca
     3001 gaggtctaca aggtagccac ttggaagcct gacaagaaac ttctgaaaga ggatttgaag
     3061 aatggccttg aaatcatcgg tgccagccta gttgaaagaa agaatttgag tataaggtga
     3121 aaataacaaa agcaacagaa attacgaatg atgatgcctg ttacctgatt tatggaaatc
     3181 caggctttgg gaaaacgact actatctcat tcataccagg gaagacacta ataatcaata
     3241 tcgataaatc agccaaagta ttagctggca atccgaacat cgatatcgca gatgttgata
     3301 cacataagat ttgggatgaa tggttgactg tcgttaagga attgcttcaa ggtgcaggga
     3361 agccatacga tactatcgtt gtggataacg tatctgaatt attcagagcg tgtcttgcta
     3421 atcttggtcg agatggaaaa aatcatcggg ttccaactca ggcagattac caacgagtcg
     3481 attttacgat tttggatagt ctacgagcct tattgcaatt aaataaacgg attgtattca
     3541 cggcttggga aacatctgat cagtggtcag atgaaaatgg catgatttac aacagggcca
     3601 tgccagatat tcgttccaaa atcttaaata atttccttgg tttaacagat gtagtcgctc
     3661 gtctagtcaa gaaaacgaca gacgacggtg aggaagtgag aggttttatc ttacaaccta
     3721 gtgccagcgt ctatgccaag aatcgtttgg acgatagaaa ggggtgtaaa gtagatgagc
     3781 ttttcgctca gagattacca gaaggaactg ataattgacg ttatcaaatc catgaaggca
     3841 ggcaatcgaa agataatggt tcaatccccc ccacgttcag gtaaaacggt cgtgatgtct
     3901 tacatcgcta agaatgcaac ggataaagat aagacggtat tgtttttcag tcaccggaaa
     3961 gaaatcaatg agcaggttca tgaggctttc aaacgtggcg gagttaatct tgataaggtt
     4021 attatcggaa cggttgggag tattgtgcga aagttagaac gattgcctca agtcgatgta
     4081 atcttagtag atgaagctca tcatatcaaa gcaaaacaat atcagacaat cttaaattat
     4141 ttcagtaatg ctactcaatt gtttttcaca ggtacgccta tccgattgga tggttcagga
     4201 ttccacgatt tagcagatga tttagtagtt ggtaaatcca tcctatggct tcaagaccac
     4261 gggaatatcg ctgaatttga ttactattcc atcaatctgt tggatatggc caaacttaaa
     4321 aaacgatcag gagaatttac caaccattca atcgatgtag catttgattc caaggcgacg
     4381 tatggtgatt atatcgatca ctacgagcga ttagccaaag ggaaacaagc tatcgtttat
     4441 acacatagcg tagaatacgc tgagagggtc gctaagcgat ttttagagca aggctaccaa
     4501 tctgctgtcg tgtccggaaa aacgccacag ggcgaacgag agagccatat gcaggcattt
     4561 agggaaggaa aactcacaat catggtcaat gtcaatcttt ttacagaagg aattgacttg
     4621 ccaaatgtag atgtctgtat tatgttacga ccgactgact cgctttccct gtatcttcaa
     4681 ttcgctatga gggccttaaa tccaagagaa ggcaaaaaag caatcttgat agaccacgtt
     4741 ggaaatcata tccggcatgg tctaccgaat gacgataggg aatggacgct ggacgggact
     4801 aagaagaaaa agaaatcttc agaacgttca acggtgacgt gtgagaaatg ttttgcgaca
     4861 ttttggagag agcagctagt agatggttgt tgtccgtact gcaaggcgga gattgtcgag
     4921 aagaaaaaca ttcaagatat caaacaagat aaatcagata ttcaattaac aaaaatcaat
     4981 caaggaatgg aatttattac cattcgaggc aaagaaatag aggtcaagac agaagaagcg
     5041 aaagtgtatc gtcgcgtcaa gacctatggt aaacgataca caagatgtca gaacttgtcg
     5101 gaattaaaag cattccgatt gcttaatggc tatcaaccag gttggttatg gcacaaacaa
     5161 aaagaattaa atttatggag ataataaaca tggcactttt ttcagtaaat tatgaagcag
     5221 cagaacaatt ttcatctatc gaagacggaa catatgaagt agttgtagct caagcagaac
     5281 agtcagcaag tcaaagtgga acagatttct tagatattcg cttgaaaatt cgtgatgatt
     5341 tccaacagaa attccgtaac aatctaattt ttgataaggt gtggatcaat aaacagactc
     5401 ttcaatatcc agaatgggca ttgcaacgct attctaaagc agttaaaatt ccagagggag
     5461 ttgaagtgaa tacaatcgag caattcttag gacttatcat tggtaaaaca ctgaaagtta
     5521 ctgtaaaaaa tgagcagtca gaatacaatg gtaagaccta cgataacttg aatatcaaga
     5581 aaatggaaca atctgagttg cctccgtatt ctggagcagt atcgtctgaa ccagctccag
     5641 ctaaagcgga tgatttagat ttaccattct aatctatggt tgggatggta gattatgccc
     5701 ttcattatca aaaactaggt tactcggtca tcccaataga caaaaagagc aaacgtgcaa
     5761 tcacgaaatt caaggataaa acatttagcg agaatgaaat taggagattt tggcacgagc
     5821 aacccgatgc gaacatcgca tggaggacaa ccgatttctt tgtcatcgat attgatgtat
     5881 cggtgactga gaacggttat gagtctttaa aagaatggga attgtctcag tatattccta
     5941 agactttaac cgctactacg ccgagtggag gaaagcacat ttttcttaaa aaaccaaaag
     6001 gcatagagtt aagtcaagat atacgagtaa aaccaggaat tgatattaag gcgaataaaa
     6061 acaattatgt cttggttgca cctagcaata atgccaaagg aagttataag tgggataagt
     6121 ccacggaaca gatggcggaa gcaccagctg agatcatctc aatcttacaa acatctaaac
     6181 aacccaaaga acttatgaat tttacaactg attacagtcg aggggagttt tcaagtaaga
     6241 ctgccaagct attcgaacaa gtcgttttcg gtttaggcga taaaggtggt agaaataacg
     6301 ccttggcgag ttttataggc ggcttactca tgcgtggtgt ggatgtggat gcaacctatt
     6361 tactagcaaa gatagcaaat cactatactc cagacagtct gccagctgat gaagtggata
     6421 gaacgtttga aagtatggtt agaaaggaga tggatagaag aggtggctat tgatttacag
     6481 aaattaaaag aagaatatcg aaacaatatt atccaacatc cagcttatat cgagaaagca
     6541 aatgactggc gtgagattcg tttggcttgt cgtgaatatc ggaaaaactg gctcgaaaac
     6601 gttaagtggg aagaaaccca gtacggtacg aaagaagaaa ataaaaaagc gcctactcgt
     6661 ttaacggagc ttgcagtcgc aaagggtatg gaacagattt tatatatcgt gaatctgcca
     6721 aatgaacggg tcgcaatcta tgatccagat aaaggctatt atcacaagga ccctagtttc
     6781 gcttataaag tcattcgctt actagaacca aatttcagtg aagcgaaatc caagaatgtt
     6841 ctcttcatgc ttgcttctac accacggttg aaccaacacg aggggttctc atgtgatttc
     6901 tcaattgggg aatacaaaga cccccgtagg tttatcttag tgaagaacgg gatatacgac
     6961 aagaaagaaa aattactacg accattcaca catgagtttg tcgcattctc aaccatcggg
     7021 acggaatacg accactttgc taagtcccat gaaatagacg gatgggatat tgatagctgg
     7081 ttacttgacc tcatgagtgg agatgaagaa ctagttcaac taatctggca ggttatctca
     7141 gccagtctga atgggaatta ctcctacagg aaatccattt ggttcgtcgg tgaggggaat
     7201 gacggtaagg ggactgtcca gcaactcata accaatctgg tcggtatgcg aaacgtggcc
     7261 agtttaaaac tcaatcagtt ttcagagaga tttgctttat cgatgattga aggtaagacc
     7321 gtcatcatcg gggacgatgt gcaagctggt atctacgtag atgaatcttc aaatttcaac
     7381 tctgtcgtga ctggtgagcc aatcttggtt gaggaaaaaa acaaacaacc gtatacgacc
     7441 gtatttaaaa aaactgtcat tcaatctaca aatgaattac cacgttttaa gaataaaacc
     7501 aatggtacat atagacggtt tgcaatcata ccgtttaaga aatcattctc aagcaaggaa
     7561 gataactggg caatcaagga cgattacatc tatcgtgaag aagttcttga gtacgtttta
     7621 aagaaagccc ttgagatttc atttgatcgt tttattgaac ctcaagcatc gattgaggcc
     7681 ttggagaatt tcaaagagtc caatgataca gtcaaggcgt tcgtcagcga atggttcgat
     7741 aaattcgaat ccactcgcct tccgtcccga tttttgtggt ggttgtatca ggaatggtgt
     7801 aaggacgaag gtgtcacaag attgacaaaa cgtaaatttg aaacgcagtt agctaagaat
     7861 atcccagaga actgggttaa gaaaaaaatc aaaccgttag gaaaattcat tccttcagta
     7921 gatgtaccga agcattattc aggttttagt tggttgaatg atgaaagtca gatacttaca
     7981 tcggggtatg aattggttac cgtttaccgt taggttaccg ttcttttttt gactacggta
     8041 acctgattta agccttatgt atcaagggtt tacctcttgt aggttaccgt attacctttc
     8101 ttttctattg aattaataaa aaaataaata atataaatat aaataaaggg gaaaggtaac
     8161 ggtaacggta accttggggc aaaaaaatga cgtaaaccgt tggtactact ggatttgtag
     8221 tggttaccgt tctaaaatca ataacggtaa cttttggagg gaaaatgaag tcagagcaag
     8281 aagtacaaaa tgaaattaga gtcgcattgt ctcaagctgg atataccgta tttcgaacga
     8341 atgttggaaa agttaagacc gcagacggca gatggtttga tactggattg ccgaaaggtc
     8401 atgcagatct gtatggtttc agaccagacg ggcagatatt ttatgttgaa gtaaaaaaag
     8461 aaaatggtcg tgtgagacct gaacaggaaa attttattga gacggttaga aaacgaggtg
     8521 cgatagctgg tgtagctaga agcgccaagg aagcgttgga gttagttaaa tgaataatca
     8581 attaaaggta gaagtacaat gtcagttttg tggggagtgt atcacgagat attgcagagg
     8641 atagtggatg ggatttgata ggaggtagga aatatggttg gagtaaccta tcaggaaatt
     8701 catctctttg ttgaattttt gaaagagcag tacgggcaag gtcgtccaga ctatattgaa
     8761 gccctgaacg acttagacgg tctggtggaa gtctcctaca gagaagctat tgaaagattt
     8821 ttagaagatg aagtacgata aacaggctga gattgacgga ctgaaacgca cgatcgagca
     8881 gacggaggca aggatagtag aactatctga gccgtgtgtc aaatcgcttg cttttagcag
     8941 gtctgaggaa cgtgacttac ttaaaaagaa agtgaaaaac tggaagaaga gaataaagga
     9001 gttggaagat gaataagcag gaattgattg agaaatataa agagctggag aatagttcat
     9061 ttgatattgc agcgattgta gtttgtcagc tagttttaaa agacttagaa cagctagatg
     9121 aaccaaaacc agtcaaagta aagcaatttg tggcggattg gtatgaagag aataaggatt
     9181 cttttgaatt taatgtttgg gattggattg ctttcaggga tgaggctaaa aaatcagaaa
     9241 atagagagtt taataattgg attaataaca ccagagaaaa tcctattcaa accctcgtca
     9301 acatgcacaa cttcggctac gaggtcgaaa aagagaagcg gtattttgtt aagattaaag
     9361 ggaatattaa agaaaatatg ttggtttatg gagaactttt gaaaaggtat ttctttacaa
     9421 aaagctttag tttagacgat gttatatatt cccacacccg taaacaacta gaagaagctg
     9481 gcctcggatg ggtgttcgat tgtgaaggaa ttgagattga ggaggtggag tgatgacaca
     9541 aacacttgaa gaaggaatga agaatcaaag taaatgcata aaagtcccaa gggaaatcag
     9601 accgtttgat atagggtatc gaatagtgaa caaatacggt caagcgctcg ctttaagaaa
     9661 tggggcaagt atattcgatt tgccttttct ggctgaaaaa gctatagaaa aagagtttgg
     9721 gaagaatgat ccagactttg acatcggaaa gcattctgtt gaagaggtcg ctattgtcaa
     9781 tttaagtaaa tttcatagtt actttgaggg ggtggagtga tgtcatgtag tgaaaattta
     9841 aaaaaagaaa aagaattgac tgctgctatt tcaaatttca agatagaaga cttacaaaat
     9901 gatgataaat tgagcagtct atcattaagc aacatcaaaa ggcaagcaag ggatctatat
     9961 gaatgcttag tatggttgca gtataatgcg gaggaatcag gtagatgagt tatgatttgg
    10021 aaatcttagc gaaaatagag agtggagatt atatttgtat tgctgaacct agatatagtt
    10081 ctccgaccta caatcttgga aaaatgttta gggtggctat ggattgggat ttcgaccaag
    10141 gcacaattta caatgttgct gatatttttg aaaatattaa acgtggcata actgaattgg
    10201 aaaggcaacc tgaaaaatat gtacaatatg aacctgcaaa taaatgggga acgatcaatg
    10261 atgcgttata tgttttgaga tcgttaaggg actgtatttt agaacaagat attgatacga
    10321 aatatttata tgtgaggtgg taaattgaaa cgaccaaaca gatacccgta cactaaaaat
    10381 caatgggttg aagaaaccgt tgatcactat acttataaaa acgatatttg ctatacaagt
    10441 cacattttag aaaatagact tactggagaa attaaggaca aggagttgaa gtgatggaag
    10501 agttaaagaa aaaagttaat gcagtataca actggacggt agaagacggg aagccgcaac
    10561 ctcccaagca agatttacca caagcggtga aagaccgggc ggactatttt tgggaaatgg
    10621 cagaagatgg tatgacgttt atgggagcga tggaatgcat ctttgctgat gaaaagccta
    10681 cagactatga tttgggagct actaagggtt ggttgccaaa atctaaggag tttgatgatt
    10741 ggattggcta ttcgccaagc atggctcagg tagttattgc agtttatttg atttatagag
    10801 gaaactaaga tgaataagca ggaattgatt aaaaaattag aggaacgaag aacaataatt
    10861 gggaattttc aaggttatgc agtttggtgg aaggatgtaa aagaaatctt tgaacaacta
    10921 gacgaaccag agataggtca cgcagatgaa gctccacgct acgtaaagaa catactagca
    10981 cgattgcgag agcatgatag agaggtttgg ttaaaggcta tcatgagcga atttgaacag
    11041 gattttagtc atgcaaaatg gcgagagggc tacgagcaag gtaaaattga gggtatggtt
    11101 gaacgtgaaa aagtcatagt tccgcagtgt gtggctgatt ggattgagga agctagaaaa
    11161 gcctgtaaag acgtggtaga cttcttcgaa tttaatttca cgaacgatga agtcggtaaa
    11221 tggtttatgc aagaactacc atttgattta gcagctagag catggcttga cggctacgag
    11281 gtcgaggaag agaagcggta tacagtagtg acgaaagcaa caaaataacc gctatattat
    11341 aatgctatgg ataagaaact attcttctct atgggcggac tagctacaaa atttactcgc
    11401 aaacaactag aagaagctgg gctcggctgg gtgttcgatt gtgaggggat tgagattgag
    11461 gaggtggagt gatgagcctt acgctaaata gcacaattgg agacttagtt ttggcaatcg
    11521 gagaaattat cgttggttct gatggtaaaa ccactacagc gatactggag atacctgatc
    11581 aaagctttta cttagagatt gcgcttaaat tgaaggagga ggtcataaat tgaaacgatt
    11641 catagctatc tggattctgc tatctgctgg attgaatatc tggcagatgg ataggattcg
    11701 agatttggaa gagaagaagc cgatggttat ctacaaggct gataacgtag gtgctgagat
    11761 atgaggagga tttggcatga taccgaaatt tagagtgtgg cattatgaat taggtagact
    11821 gatgtcagtc aaatgtatgt tttttcagga tagcgagatt gaagaatttg agttaaacga
    11881 tgctttaatg aatgattaca ttacagctta tcctgacgaa atcgaactca tgcaatcaac
    11941 aggactcaaa gacaagaacg gcaaggaggt attcatcggt gacatcgtta aatgtacaag
    12001 aggatgtcca catgaagtgt atttagaaaa agaatacggt ggcacataca taggcggaat
    12061 gcctgctata tatctaaagg gattgctaag tgggtatgcg tggactgaag acgaagaaat
    12121 catcggtaac atctacgaaa atccggagct tttggaggtc aacgagtgag atattttaaa
    12181 atcctatgta ttgttttatt cgcatcctta ctcgtagcat gtcaccaggt ttcgagtggg
    12241 acagtggtag ataagtacat tgatgaacct cacacaacgt tcatacctgt tataaatggt
    12301 aaaagttcgg tacttgtgcc aaccagaacc aaaagaaaat acattctggt cgtttcagga
    12361 tatgcaggta ataagcaagt tgaagaaagg tttgaagtga cagcaaatga atacaagcac
    12421 tatgaaattg gcaatacttt tacaaaagat gctgttttag agaataagga aggggataaa
    12481 taatgagacc aaaaaaatat ccgtattcag gaagaagaaa aaaacaagat acatcgtcgc
    12541 cattattttc tacacgacca atttttaacg agattccaat tgtagaagag gtcaaagttg
    12601 atctcggagt tgaagctaat gttggacgtt cttatccaga aatgttaata catttagata
    12661 tttctggata tgggaataga atacattctg tacatcgttt ccctggtatc ttccttactg
    12721 ttggtgagtc aatccaacta aagatgcttt tctataaaag acttaaaaat tttaccgcag
    12781 atcgtttttt gacctttaga gaatctgact ggaagttctt tatacgtggc ctggtcaacg
    12841 aatttgtgca ttaaaaaagc caagacaccc tctgtctcag ctaaattcct attaagatta
    12901 ttatatcaca aaggagatag agagtgaaca aggctaaaga gctattgaaa gaattacaag
    12961 accttgacat ggacatccaa agccgtatag atgaaatcaa tgaacttgag gcaggtttgc
    13021 tctcaagtcc taagtggtca gatgtcaaag tcaaaggtgg acaaactaga aaagttgatg
    13081 atgtctatac tcagctggta gtgatgaaag aggctataga gcaggatact aaagaggtta
    13141 ttaacagaaa acttgaactt ggtagaatga tcaacaggct taaaaatcca aagagcaggt
    13201 ctgtccttag aatgacttac attactaaaa cctacattga ggatatttgc gacaatttga
    13261 gaattagtaa ggcaacttat tacagattac gcaaacaggc tgagtccgaa ctagaggaga
    13321 caatcataga caaagtaagc taaagtgagt gcgcatgaag tttaaaatct gttaaaatgg
    13381 tagtatcaag aattaagggt aaggcagtaa gccttccctg acatggagag ttggcagagt
    13441 caggttgaat gcgcccgttt gctagacggg tggtcgccta tgtgcggtcc gtgggttcaa
    13501 atcccacact ctcctttgag tatttgtgtc ccataatggg gtaggcagta ggcttagcat
    13561 tcacatatca ctcattaact taaaaatggt tgcggagcga ctagaccttg catggttgcg
    13621 tagctactta tatcctaggt aagttataag ctagagggtt tgattccctt agaggtttta
    13681 aacgactaca aaaaataaaa aaagaaagca tttcaaaata gatttctaat taacacgcaa
    13741 gtctgtagta tgcttgcagt aagaacatag ctcaagtggt agagcggtag atttttaatc
    13801 tattggttgc aggttcgagc cctgttgttc ttatgagagg tcttgaaaag gtcgcacatc
    13861 gtgtggcttt tttgattgtt tgaaaggtgg tgatggaaaa ttgagtggat tgagaataaa
    13921 acaaaagaga tttgcagatg agtacatcat ctcaggtaat gcgacggaag cctataagaa
    13981 agcaggttat cgtgtttcta gtgatagagt ggcaggcgtt gaaggacata agttactaaa
    14041 gaatcctaag attaaaagct atatagatga acgactgaaa cagcttgatt ctgaaaagat
    14101 tgcggatcag caagaggtct taggttatct aacttcagtc atgcgaggag agacgcaaga
    14161 acagaccttg ataagcattg gagaactagg gcagacgatt acggatatag atgtaggagc
    14221 taaagataga atcaaggcgg ctgaacttct tggtaaacgg cataggcttt ggacggataa
    14281 ggtagaggcg gatgtttctg gaacggtggt gtttgcaaat gagtcagaca taccagatta
    14341 agcaaaacga tattgtcgtt gacttaccta agacagtagg agctgggtac ggacagttct
    14401 ggcgctcaag aagtctttat cgtgtagtca aagggtcccg tggttcgaag aagtccaaga
    14461 caaccgcttt aaactatgtt gtccgtcttt tgaaatattc ctgggccaac ttgcttgtta
    14521 ttcgtaggta ttcgaataca aataagcaat caacttatac ggattttaaa tgggcgtgta
    14581 atgtgttggg ggtgactcat ttgtttaaat tcaatgagtc tttgcctgaa ataaccataa
    14641 aagcgactgg tcaaaaaatc ctattccgtg gtttggatga tgaactcaaa atcacatcta
    14701 tcacggtcga tgtcggcagt ctttgttggg catggttcga ggaagcatat caaattgaga
    14761 ctgaagacaa gttcagcacg gttgttgagt ctatccgtgg tagcttagat gtacctgatt
    14821 tctttaaaca aatcacagtc acatttaacc cgtggaatga gaggcattgg ctcaagcgtg
    14881 tattcttcga tgaagagacg agacgggctg acacattcgc tactacaacc acttacaaat
    14941 gcaatgagtg gcttgatgaa gtcgatatca aacgctatga ggatttgtat catacgaacc
    15001 ccagacgtgc tagaatcgtt tgtgatggcg aatggggagt tgctgaaggt ttaatctacg
    15061 aaaacgtgac tgtcaaggat ttcaataaag atgaattact acaagattca gctaataagt
    15121 tatgtatcgg tcttgacttt ggttttactc atgatccaac tgctttgtgt tgttcgttga
    15181 taaatgacac gacgaaagag atttatgtct ttgatgaggc gtataaagtc ggattgataa
    15241 ccaaagaagt tgcgaagatg ataaaggaca aaggttatca tcgctcacaa atcattgctg
    15301 atagcgctga attacgactg attgaagagc tcaggtcaga acatggcata tctagaataa
    15361 aagagagtcg gaaaggtaag gatagtatta tggcaggtgt atccaaattg caaggatacg
    15421 ctatttatgt gcatccagat tgtaaaaaca tcatggatga attttatagt tactgctacc
    15481 agcgagataa agaaggcaac tggttgaata aaccagagga taaaaacaac cacttgatgg
    15541 acgctttgcg ttacagcctt caatgtatcg aaggtgggaa agcaaccgtc cgcagacgtt
    15601 ctgattatgg tctatagaga ggaaagacat gtaccaatat ttaacctatc cacgggatgg
    15661 atatgatgag ggttctttga agaaagacct gatttacaaa ttgataacga tacataacac
    15721 tgaaagttca catttgaaga agcttaaaag ctactacatg ggcgagcatg ctatcttaaa
    15781 acacacgaga cgcaacgtga acgcacccaa ttacaagacg gtagctaatc atgccaagga
    15841 tatcgcagac acggctacgg gctattttat gggcaatcct atcaagtata acaatactgc
    15901 tgacggtgat attgatgaac tacttacagc ctttgatggt gctgagattg accaagtgga
    15961 tgcgcagaac gcactaaata tggctatcta cggccgtgct tacgagtaca tctatgctaa
    16021 agagggtatg actgagttgg attcaactag tattgatccg gagaatactt tcatggtcta
    16081 cgatgatagt attgagcgga agcctttgtt tgcggtctat tactatgaag taaaagacga
    16141 tacgaaagac actaccaagc accaggctga ggtctttacc gaaaatctgc actatcacat
    16201 ggtgctgaga agtacagatt caggaacaac tcagagcgag gaggcaacac ctcacaacct
    16261 tggtcaaatc ccaattatcg agtatcgcaa taatcacttt gcgattggcg actacgagca
    16321 acaaattagc ttgatagacg cttataattc cttgatgggg aatcgtgtca atgataagga
    16381 acaggctgta gagtctatac ttgtcttgta tggcacgcag ttagcagaca ctccagaaga
    16441 cgctaaggta gcaatgaaga ttctttctga agaaggtctt ttggaattgc cgggcgatag
    16501 tgcaagggct gagttcttga agaatacgct ggacgaaagt gctactgaaa tcttgcgtac
    16561 agctcttaaa gaggacatct acacatttag ccatgtgcct aatttgactg atgagaattt
    16621 cgcagggaat acatcaggcg tagccatgga atttaagctg atgggccttg agatgattac
    16681 taagaccaag gaagcgaact ataagcgagg attgcgtcag cgtattgcga tttttgctca
    16741 ttacttaggc atgaagcaga ttgctttaga gtctcattca atcgttccac aattcagtcg
    16801 tggtttgcct aagaacttgt tagaaatctc tcagattgtg aacaatttgg aaggcaaagt
    16861 gaccaataga cagcttattt ctctcttgcc gtttgtggaa gaccctgacg ctgagctgga
    16921 agccttggaa gaagagaaaa agaagaacat ggaagacatg ccgatgttca acaaagacaa
    16981 cacgaaaccc gaagacgagg tagaggatga agaatcagga gtattgggcg aagaggaaag
    17041 ccaatctgat ttaccagcag atggacaagg ccgaaaagca ggcagaccag ttcgataagg
    17101 tctatcagga agctaagact tacttggata aggaaatcaa taagattttc gataaattcc
    17161 aacgtgatta tggtctaagt caggtagaag ctagacaagt cttgaagaac atgaaagaca
    17221 agaaaaatct gaatgaactt cgtaaagtac ttgaagcgag accgaatgac ccgaacatcc
    17281 aaagattact agctgactta gacagcccag cttattcttt ccgcatgaag cgcctagagc
    17341 gtttgagcga cgatttagat cgtatgcgtg aatctatcta tcattcggag aagacaggct
    17401 cagatgcctt ttatagcgac ctgatgaagg atagttacta caaggctacc tttgacctac
    17461 aacagcagac aggactagca tatggctttt ctgggcttcc tgagaacgag attaaacatc
    17521 tacagtcttt cagttgggta ggtgacggaa gtacctactc tacagacatc tggaagaata
    17581 cggggaagct tacttctagc ataaaagatg aactacttat gagcctcatg acaggccgag
    17641 atacacgaga aactgcacaa gcaattgctg agaggttcaa tgtaggtcag aacgatgcaa
    17701 gacgtttggt tcggacagaa tcagcctttt ttcataacca aatggaacta ctcagctatg
    17761 aagaagcaga catagaaaag tatatctttg tggccgtctt agacaagcgt acatcacgca
    17821 tttgtcagga gcatgacaat caggtctatg atagggataa ggctgtccct ggtgtcaatt
    17881 gtccgcctat gcacccttgg tgtaggtcta ctactgtcgg atacgatgag gacgcagact
    17941 acagcaagtt gaagcgcaga gcaaggaatc cagagacagg taaagttgag tacgtgcctg
    18001 ccgatatgac ttataaagag tggtatagca agtatgttgc gaaagacggg gaaaaggtgt
    18061 ataatcaaga tacaagagaa gccaaggcga aattttatag cgaacaacta ttgtccaaaa
    18121 tttcaggagt tgagccaaaa attacaagtg atatgcaacg tatcgcagga gaaaacaaat
    18181 tggcaggtct tgaatttagg aagaaaacag ttgagtcatt atcacgtaaa attacaacag
    18241 atagccaagc tgaaaatata agtttatcaa aggctacaag taaaattaac gatgctttgc
    18301 ggtatacaac tattttcgat cccgatactt tcgcaaaaga gtatttgaag atgaaacaga
    18361 agcttatcgc agaaggttat aaaattgtaa aagtaaaaaa cacttggcta gtagatggac
    18421 catacaaagg tgtgaataca gtcgttgaaa aagatggtat caactttgaa atgcagtatc
    18481 atactcagga aagtttcgac ttaaaaaatg gttcattaca tgaactctat gagaagtatc
    18541 gtgatacgaa tacatctgat ctagaacgca tgaaattatt taaggaaatg cttgatttaa
    18601 gcaatgggct tgagattcct aaaaatatag agagggtgaa gtgatatgaa agatattaaa
    18661 tactaccgca caacgacgaa caatgctcaa gtacttcgtt tgattgatgg tgtcatgcaa
    18721 gtttttgaca ttgaaaaaaa gtgggttaat agcatggatt ggtttaataa aatctttttt
    18781 aatgacttta cggattttga agaaatttca gaaaatgatg catttactta tattgacagg
    18841 atggtagcgg catgattgat attgccttgg ctatcgctaa aaaagcacat gcagggcagg
    18901 tagataaagc gggtgttgat tacatacagc atcctctcta tgtggccagt caagtcaaca
    18961 ctgaacaaga aaaagctgtc gctcttttac atgatgtgat tgaggatagc gatataactg
    19021 ctgccgattt attcgcgtct ggcttgtcaa atgaagttgt tacagcggta caaattttga
    19081 caaagaaaaa aggtcaaagt tatcaagaat atcttgggaa agtaaaatca aataatttag
    19141 caagagttgt aaaacttgca gatttgaaac ataactcaga tttatcacgt ttgaaatctg
    19201 ttaccaatac agactacgag cgtgttaaaa aatataaaaa tgcaattcac tacttaagca
    19261 cctagagaga tctaagtgct tttttcgtac ccagaaagga gtgaggaatg aaataccgta
    19321 aaaaaccagt agtggtcgag gctgtgcagt ggaacggcaa taaccataaa gaggtaattg
    19381 actttgcaga aaataagatt tggtttgatg cacttgggaa tatatggatt gctacacttg
    19441 aaggtgatat ggtagctaaa aaaggggatt atattatcaa aggcgtgcaa ggagaatatt
    19501 atccatgcaa gctggatatt tttgcagaaa catatgaaaa aacggaggaa taaaatgtta
    19561 gaaaaagcaa aacaattggc atcgcaagaa ttttcacgct tatcaggtcg tgaaatcaaa
    19621 gcagaagact gctttgtagt ttggtttagc aagaccctgc aaaactggaa agctcttgtt
    19681 agtacgaacg caattacatc aagcgaacct tgtggaaatt atgcagaaat cacgcataac
    19741 ggagataaga aagagactta tgtggatgtt tacgccaaag tttcaaatcg tgccattaaa
    19801 gattaggagg tgatccaaca tcttgacttg caggaataga ctgctataaa ttgctataaa
    19861 ccgcatcgaa atcgaggcgg ttttcttatg ccctaaccgt atggaacccc gtacggtttt
    19921 tatattgtcc aaactgtacc gatgacatta aaagctgtac tgttccgtcg ccggacgtaa
    19981 aacgagatta tcgagtggcg acgtaatcgc tggaggacaa ttatgtcaga agaaatcaat
    20041 gcaactgtat ctactgaatc aactgagact gtcgacactc aaggaaatgt tgattcagtg
    20101 caggaagaaa agcacgaacg aactttcact cgtgctgaaa tcggtaagat gctatctgcc
    20161 gaacgctcta aatgggaagc tgagcaagaa gccaaggaaa acgaagctaa gaaacttgcc
    20221 aagatgaacg ctgacgagaa acaaaaatat cagttggatc agcgtgagca agaactagct
    20281 gaccgtgaga aggctattgc tcgcaaggaa ttgaccgcag aagctaaagc aatgctaagt
    20341 gaacgtgact tacctgttga gttagtaaat gtagttgatt tgacaagcgc agagacggta
    20401 tcgcagtctg tcgctgtatt gcagaaatca tgggagcaag ccgtgcaaaa aggcgttcaa
    20461 gaaaaactaa aaggcggagc tccaatgaaa caagcgccag tcgatagtga cggtatcaca
    20521 aaagaagaat ttgctcgtat gggttatcag agtcgaaatg agctctatca aaagaaccca
    20581 gagctttata agaaattgaa aggataatag aaaatgacag caggacaaac taaattagcc
    20641 actatggtta atccagaagt aatggcggat atggtagccg ctaaattacc taaattgatt
    20701 aaatttacac cgctagcgta tgtagagaca aagcttgaag gtcaaccagg tagcacttta
    20761 acagtgccag catgggagta tgcaggagac gctactgaaa ttgaagaagg ccaagcaatt
    20821 acgccagacc aattgactac taaaaagact actatgacca tcaaaaaagc aggtaaaggt
    20881 tatgaaatta ccgatgagtc tcttttgtca ggtcttggtg acccactagg tcaagcgact
    20941 taccagcttg gtttagctat tgccaacaag atcgataatg atttggtagc ggtagctaaa
    21001 actgcaacac aacatattac agaaactcca acaactcttg aggcaatcga taaagctcta
    21061 gatatctttg aggacgaaga agatgcacag tatgttgcta tcatcaaccc taaagatgct
    21121 actaagctaa aaactgcagt agcaaaagaa tggattaaag gttcagagct tggagcaaat
    21181 atggttgttt ctggaacctt cggtgaagtt gatggtgtgc aaatcgtgcg ctctaaaaaa
    21241 gttgatgaag gtaaaggctt ccttgttaaa gtgtcaccaa gtcaaactca gacagacgac
    21301 gctaacaaat atggagcttt tgttatcttg cttaaacgtg atgtggctat cgaaacagac
    21361 cgcgatatct tgaagaagac taccgtaatc acaggtgatg aacactacgg cgtttacctt
    21421 tacgacccta cacgagttgt aaaattcggt ggcgcgtaag aggtgacgat atgagtttat
    21481 tgctacgacg tcattatatt caagaagagc aggttaacca gtattctgat ttagagaata
    21541 aaactctaga agagttgaag gatctagcga aagaagcagg tgtagcaggc gcttataagt
    21601 tgacaaaagc cgaaattgta gaagttttgg aggaactaaa aagtgaaatt taaaatcaaa
    21661 caagatttct atgattggga atcaaatgtg aaacgactgg caggagagga acttgagatt
    21721 actgaggagc gctatgctga gctggctgac aatattgcca gcaacggtgt cactatctca
    21781 gacgttcttg agaaaatcct ccctgaacct gagttcttag aagaggattg atatgtctat
    21841 agagttgctg aagaaattaa caggcgaaga agatactcag cttctcatgt tgctccaaac
    21901 gagggctaca aatcttatct tgtcagagac taatcgcaca tctttgacac ctgctttaag
    21961 tcttttgata cctgaggttg ctatcgagct ccacaaccgc tcaggagcgg aaggagagca
    22021 ttctagaacc gagggtggta tagcagtagt ctacggagaa aacggcctat ctacgggtct
    22081 tctacagcga atacgcatgc acaggctagc aagggtggca ggtcatgttt ttgaagcaga
    22141 gtagactgaa accctatcca atgcgacggt ttgaaaagac tgtcactgag gaaggtgtcg
    22201 cgaaagaagg gtatgccaag gaagctgaga cagtccgcct tgaattgtgg ccagctagta
    22261 gcaagctaca atctgaattg tatggcgagc gtgtcaatga tattttgaac gcaaatgcca
    22321 acaagtcagc tactatcaaa gtaaaagatg gtgtgtgtat cgatagtcag acggaagtga
    22381 ctcatagggt tatttctaaa aaggtctaca cacatcatca agttttggag ttagagcgtg
    22441 tcagggctac taggggcaga taggcttata gctaaatgta gacggttagc tagtaaaaaa
    22501 actggcgagg atatcgtctt acgtgcggta cacaatgctg ctataaaagt tgtccaagct
    22561 gatgcaagaa gactcgtacc agtgaatgat ggagaactta taactagtat caaaaccaga
    22621 gcaaaaatgg acggagatag ggctataggc gaagtttaca ccaaccttaa atacgctcct
    22681 tacgttgagt ttggaacggg acctaagggt caggctagcc attctggtat ctctccagag
    22741 gtcagcgtga cttacaagtc tcatccttgg tatgtgcatg aagaccaaat caatgtagga
    22801 tcttaccact ttcaaaagat tggggagttt tacaagatgt atggtcaacc tgctcagcct
    22861 tatctttatc cagctttgag agacaatcaa gagcgtgtgt ctaagaatat ttcgaattat
    22921 gtccgtagaa agataagaga acaaatacaa tgatcaatat caagcctgtt atttataaag
    22981 aattgcaaaa ggttgcagat aatgtgaccg atacttatcc tagcgattgg gagactttcc
    23041 cagtcgttat ttttttagaa gaacaaaaca agccaggtga ttggtttgac gacaaggaac
    23101 aaaaatcctc tatccgctat aaggtggata tttttgatga taccagcact agtgagttag
    23161 ctgttaaaat caatcagatt tttgagtctt taggtttgcg aagaaccgac tgccaagacg
    23221 tgccagaccc gtctcatttg agacataagg tcatgcgttt tgaaggtgtc gttgacttag
    23281 attcagagct tgtttttcaa tttagaatgg agaattaaaa tatatgttag caaatggaat
    23341 tacgctttct tatagcaaaa caaaaggtag ctatactaag cttgttggat tgaaagaagt
    23401 gccagagttt ggtattgaac tcgaaaaagt agaaaatact actcttgaag atacggtgaa
    23461 gaagtacgag cttggtattg gggacgtagg agaacttgag tacaagttct cttataataa
    23521 ttcaagcgca actgctcctt accgtgtatt gcgtaaggca gcagacgaca aggaaaaact
    23581 ctacttcgaa caagcttatc cagacggtac taaggtcaca tttgaaggtc aagtatccgt
    23641 aaaacttggc ggtggcggtg tcaatgccgt tatcgatttc acccttaaaa ttgctttaca
    23701 gtcagagttg gaatttgcag atggtgttgg aggttaatta aatggcgtta aaatacacaa
    23761 cttggaaagt tactgacgaa aaagagttga agctacgttt gacatctcat caagctgcaa
    23821 ctgtggaaga aaaaatcggc atgaacttgc tgaagatttt catgcctgaa gcaggcgaag
    23881 agttcacttt gccacctttg aaagttatgt tgttgttagt tcatggagcc ttgcagcagt
    23941 atgaacatgg gtattccttt gaagatgtct acgacctata cgatgaatac gtggacaatg
    24001 ggggagacca aacaactttc atgacagagg ttttaatgcc actatttgaa gtatcgggtt
    24061 ttactccacg aggaagcaag aacaagaaaa cttccaagaa gaaaatgaca gtagtcgagt
    24121 aatcttaaca gtaacgcaga ttattgagag gctttatcct atgtttttgg acattggagg
    24181 caagcctctt gatttttggg atttgacggt gcttgaaatc agagaaatga ttgaaagcta
    24241 caaccgtgtc aaaacccaag agcgtaaaga gaagattatt gactcataca gactttcgca
    24301 gatgatttcc aatcacgttt ctttattact gtccaatgac gctaagattg ttgagttctg
    24361 ggagtatgcg cctgagttat ttgtagaaga acaacaagcg gtagaactgg aacgacagaa
    24421 acaagcactt ttgttgcata aggaacggat gcgtgaattt gcagagagac ataatcgaaa
    24481 aaggaaggag gaagtaaatg gcaactcttg atgaattgaa agtcatgatt gacgctgaga
    24541 tagcgccttt caggaagaag atgaaagaag tcgagaatca ggtcaaagga acatctgacc
    24601 aagtgaagaa tgccactgcc aaagttcgtg aacagtcgaa ctctatcggt agtgcgtttg
    24661 gtaagctagc taagttcgct ggttttgcaa tccttggtaa gaaattactt gatgttggga
    24721 tgtattcaac gcagactgct cttgaagtgt cagcggctat gaaccaaatc aagcgccaga
    24781 tgggcgagag ttcgcaatct ttcttaaaat gggttaacga taacgccaac gctatgaaca
    24841 tgggtgtggg tgaggctact aactacggtg cggtctactc aaacttattt tctgggttta
    24901 tcaaagatac caacaagcta agcgcctata ccgctaagat gttgcagaca tcggcagtgg
    24961 ttgctgaagg ttcagggcgc acgattacag acgttatgga gcggattcgc tcaggtttgc
    25021 tagggaacac cgaagcaatt gaggacctag gaatcaacgt caatgtggct atgattgagt
    25081 ctactgaagc ctttaagaag ttcgcaaacg gacagagctg gcaacagttg gattaccaaa
    25141 cccagcaaca aatccgcctt atggctattc tggaacaggc tacagccaag tatgggaata
    25201 ccttgtctaa ttctgtaaat ggtcgtatca gcctatttaa gtcgctgatg aaggacgcag
    25261 cattgaacct tggtaactct atgttaccga ttatcaatgc cattatgcct gtcttgaact
    25321 cttttgcgat ggtcttgaag aacgttactg ctaaactcgc tgagtttatc gctttgatgt
    25381 tcaacaagaa agcaacagtg aaagatggtg ttggtggagc agttggagac atgggtaacg
    25441 ccatgaaaga cgctgcaggc ggagcaggag accttgctga tgcagtagac gacgctggag
    25501 attcagcagg aggacttgct gacaatcttg gagactccgc caaaaacgct aagaaagctg
    25561 ctaaagagtt gctaggtctt ttgggatttg atgagattaa catcttgcaa aaaccaaaag
    25621 atgacgacgc aggcggttct ggaggcggtg gcaaaggtgg taaaggaaag ggaggcggtg
    25681 gcggaccttt caaagacatc ttgccagaag tcgagttgac cgacatggac aacaaattca
    25741 agagcatttt tgatggtctt ggagataagc tcaaagggtt gtttgacctc ttcaagaaag
    25801 gttttgatgc agcatttaga ccagaaggta taaaacgcat taagactgcc ttagaccaaa
    25861 tagctaagac aatgggagaa atcgtcactg acccaagggt tgtgaatgcc tttaaccgaa
    25921 tggctgagaa aattgcttat gctttagggc aagtgacagg ctcaataacc actatcgggc
    25981 taggtatcgg tgttttcctt gccgaaagta ttgcaaatgg ccttggaagg caaaaagaac
    26041 gcattatcag ggcgctagtc gctttgtttg ataatgttgg taacctttcc gaggcagtag
    26101 gaaacatagc tcaggacttt tctagtgctt tctacgacgt cattacctca actggtgcgg
    26161 ttcgtatcgg tagcgctatt gtgtcaactc tgttgagttt gacatctacc attgttgaag
    26221 ttggtagtaa attagcagga agtttgttta aaggttttga aaaagtcgtt gtgacaagcg
    26281 ctcctaaaat ttcatcagtc ttccaaagtt tattagatac tgttgcgcct gtatttgaga
    26341 gcattgaaag gtctgttaac aaatttggcg atggcttaag tcgtgtttat gatgaacatg
    26401 tagtccctgc tattaactct attgctaatg cttttaatgg gctaattgac attattcaga
    26461 ttctctggga gaattcctgg caaccttttg ctgagttttt atcaggagta ttcggtgtta
    26521 gtattgaagg aatttcagat ttattaggag gtggcctttt agccactttg ggactattgg
    26581 cggatgctat taagttagtg gcagatggtt tcaccgtttt ttctgactgg tgtaaagaaa
    26641 acaaagaacc tatcgtagct ttgataacaa cttggcaaac gattaatttc ttatcatggg
    26701 cagaacaagc tggaggactt gcaggagcat tcagcttgtt aggtagtaag gtctctttga
    26761 ttgttggagg gattaagaat ctaggtcttg ctattaaagc attgacattt gataagttgg
    26821 tcagttttgg tgaaacaatc tatttgaaca ccttatatgc aaaagatttt gtggtcaatt
    26881 caggtaaaac aattgcacag ctaggaaaaa ctgctttaga acttggtaaa tcagctctag
    26941 catggactgc tcatgcagcg aaaatgggat tagcaaccgc ggcgaaattt gcacattctg
    27001 ttgcaacagg agtcgctaca gctgcaacat gggcttttaa tgcagcgtta gcagttttga
    27061 caagtccaat aacatggatt attgcagcaa tcgcagcctt aattgctatc ggtgttttgc
    27121 tctatcaaaa ctgggacact gttgttgagt ttgctaaaac tgcatggcaa ggactatgtg
    27181 attttatcag tggtatttgt caagcgattg gcgaattttt cagtggtcta tggacgaaac
    27241 tacaagaaat ctttgagccg ataggtcaat ggtttggcga gaaattccag caagcatggg
    27301 acgccattgt aaacatattc tctggcatcg gagagtggtt ctctggtgta ttccaaggtg
    27361 catgggacgc tatcgttaat atcttcacac caatcggctc atggttcgga caacgttggg
    27421 cagatgtgac taatgcgtta tccagtgttt caaactggtt tggtgagatg ttcactaatg
    27481 cttacaacgc agtaaaagat gcttttagtt ctattggcga cttctttaaa ggcgtttggg
    27541 atactgttaa aagtatcttc gtaaatgctg gtcagatggt cggagaggca gtaggtggag
    27601 cgtttaagag tgcggtcaat gcggttcttg gaacgattga aaatgtagtc aatggcttca
    27661 tcggaatgat taatggagtt ttaggcgttg tcagaaactt acctggtcta ggatgggttg
    27721 gtagtgtaag tacagttagc ctccctcgtc ttgcccgtgg tggtatcgtc gatagtccaa
    27781 caatcgccat gattggtgaa gctggtaaag aggcggtcgt accacttgaa aatacaggat
    27841 ttatccaaac acttggacga gtagtcagca gtgcggtagt aaatgccatg gctggtgtta
    27901 gtccacaagg tggattctct ggcgacggcg acatcgttat ccaaatcgca ggccatgagt
    27961 tcggacgggt agccatccaa gaaataaaca aggaacatga acgagcaggt caaaccttgc
    28021 tcaagattta ggaggttaaa tggcacaatt gacaatcaat ggggtggctg tgaagcctcc
    28081 caaatatttt caagtcggta ttcaagatat cgatggagag acagggcgta atgccaatgg
    28141 cgacatgatg cgtgaccgta tcacgaccaa acgcaaacta gactgtgaat ggggtatgat
    28201 gactcaggga gaattaagtc agcttttaca tgctgtatca tctgaatttt ttgaggtatc
    28261 ttatccagac cccatggatg gccaagtcac aaagactttc tatgtcggtg ataggacagc
    28321 tcctagctat acctttactg agaagtttaa accttggtct ggcgctaaat ttaatctggt
    28381 agagaggtaa gaaaatggac gctttaacta gacgacaatt tgacagagcc atgtttgcca
    28441 aggaaaggac gctggctatt cgtgttggtg attatgcttc acgggatatc aaagaggcta
    28501 gttttgagta tggctacatt aagggcgata cttataagcc tggtggaacc tgcgctggta
    28561 gcggtaaaat tacctttacc agtatcatta ccacgttcaa taagctggat accctgcacc
    28621 ctgagattgg tctactggtt ggggatacct accagtgggt caagatgggg gaatacttca
    28681 tcaacgatat tgagattgac cgaaaccgaa acactaccac gcttgaactt atggacggta
    28741 tgtttaagct caatcgtgag tacgtgacgg acttgcattt cccagctgaa gtacgagagg
    28801 ttattcagga aatctgcctg aaaacaggca ttgagttagc gaatgactat ttcggaatca
    28861 gcgcgatgcg ttatcatatt gagcaagttc ctgagggcaa gaaactttcc tttagggata
    28921 tgctgagcgc tatgactcag atgattggga tgtcttgttt cttcaacaga gaaggcaaga
    28981 tggaaatccg tgatttgact gagtccaata tcacgatcaa cgctgacagt tacttcttgc
    29041 atggcttgac caagagtgag attgagtatc agatagctgg tatcacttgt aagacggaca
    29101 agaagtctct gacggtcggt atgaagacag gccggtcttt ggaactggac aatgtcttca
    29161 tgacccagag cgctttaaat gacctgtatt acaaactgaa aaacctaact tactatccgt
    29221 ataatctcaa ctaccaaggg catttactgc ttgaggttgg gcagtgggta accattcaga
    29281 ccaacaagaa agagactttt aaagttcccg tgttaagtca gagctttacc tttaaaggtg
    29341 gtctgagagg tcgtatcagt gcagatagta aggctggaaa cgatacccag tattcttacg
    29401 agggtacgat taccaagcag ataaagcaac aagatggcat tgaagcgaaa atccaagcgc
    29461 agattgaagc aacagataaa gattttgacc aaaaggtcga caaaatcaaa aaagacttta
    29521 acgatcaagt agaactggcc aaagccagag ctgaagaagt caagagagaa ctgtctgaca
    29581 ctatcaatca gcgatttaat agctttgaca acgggccatt gaaagaaact aagagcaagg
    29641 ctgaggaagc tttgcgaaat gctggcgcaa gtaccctgct tgcacaggaa gctaagcgga
    29701 ttgggctgga ttctgtcgct agacttgaag cgtttaagtc gcagactacg agcgcacaaa
    29761 cggctctgtc gggtgacttg gacgctctga aacggactat cgcgaatgat attcgaccga
    29821 agcaagcaca ggctgaagct gagattgcca agcaagttga agcacttagc cagactaaaa
    29881 atgaactggc tggcgcaagt accctgcttg cacaggaagc taagcggatt gagctggatt
    29941 ctgttgctag acttgaagcg tttaagtcgc agactacgag cgcacaaacg gctctgtcgg
    30001 gtgacttgga tgccctgaaa cggactatcg cgaatgatat tcgaccgaag caagcacagg
    30061 ctgaaactga gattgccaag caagttgaag cacttagccg gactaaaaat gaactggctg
    30121 gcgtgaagtc agcgcaagcg acgtatgagg agacgacgac tcgtagactg tcagaactga
    30181 ccaacttggc caatggtaaa gccagcaagt cagaactcac gcagacagct gaggagctgg
    30241 ctagtaagat agcgagtgtg caggcatccg gtcgaaatct attcttgaat tcactattca
    30301 agcaggatat tccaaaaaca ggaatttgga caacgagtac atatacggct actatcgata
    30361 gcgaaagtaa gtatcttgga tataacgctc ttaaaattat aggtctgaat ccatctgg
//
LOCUS       DAVHIU010000013        27266 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVHIU010000013 DAVHIU010000000
VERSION     DAVHIU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.013"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            336..1499
                     /locus_tag="VVN06_000491"
     CDS             336..1499
                     /locus_tag="VVN06_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU5562095.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1496..2173
                     /locus_tag="VVN06_000492"
     CDS             1496..2173
                     /locus_tag="VVN06_000492"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU5562096.1"
                     /translation="MKLIVSVMPRSLEEAQALGATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2163..3017
                     /locus_tag="VVN06_000493"
     CDS             2163..3017
                     /locus_tag="VVN06_000493"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5562097.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLYALENVSELQARIVESDLLVNATSVGMDGQSSPVPESINL
                     PETILVADIIYQPFETPFLKWARSQGKPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3036..4103
                     /locus_tag="VVN06_000494"
     CDS             3036..4103
                     /locus_tag="VVN06_000494"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5562098.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCLAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELDGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGVRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEKKGLMPAGITQSITDMCQKFGLPVDYENWDVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4113..5279
                     /gene="aroC"
                     /locus_tag="VVN06_000495"
     CDS             4113..5279
                     /gene="aroC"
                     /locus_tag="VVN06_000495"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5562099.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINGDLKRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVVNKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5289..6392
                     /locus_tag="VVN06_000496"
     CDS             5289..6392
                     /locus_tag="VVN06_000496"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU5562100.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6403..6741
                     /locus_tag="VVN06_000497"
     CDS             6403..6741
                     /locus_tag="VVN06_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU5562101.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKTVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6833..8116
                     /gene="aroA"
                     /locus_tag="VVN06_000498"
     CDS             6833..8116
                     /gene="aroA"
                     /locus_tag="VVN06_000498"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5562102.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGASVIIEKECTRNHTEDMLKQFGGHLSVDGKKIIVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8109..8585
                     /locus_tag="VVN06_000499"
     CDS             8109..8585
                     /locus_tag="VVN06_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5562103.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDTNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8582..9430
                     /gene="pheA"
                     /locus_tag="VVN06_000500"
     CDS             8582..9430
                     /gene="pheA"
                     /locus_tag="VVN06_000500"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5562104.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSTEEYGLELIA
                     EDIQEMEANLTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9427..10701
                     /gene="psr"
                     /locus_tag="VVN06_000501"
     CDS             9427..10701
                     /gene="psr"
                     /locus_tag="VVN06_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU5562105.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10980..11825
                     /locus_tag="VVN06_000502"
     CDS             10980..11825
                     /locus_tag="VVN06_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5562106.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDTVIADMDKSYTKYK
                     GEYGG"
     gene            11815..12918
                     /locus_tag="VVN06_000503"
     CDS             11815..12918
                     /locus_tag="VVN06_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5562107.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTRNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12920..13906
                     /locus_tag="VVN06_000504"
     CDS             12920..13906
                     /locus_tag="VVN06_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5562108.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13922..15355
                     /locus_tag="VVN06_000505"
     CDS             13922..15355
                     /locus_tag="VVN06_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU5562109.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLVVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15384..16418
                     /locus_tag="VVN06_000506"
     CDS             15384..16418
                     /locus_tag="VVN06_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5562110.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16618..17355
                     /gene="mecA"
                     /locus_tag="VVN06_000507"
     CDS             16618..17355
                     /gene="mecA"
                     /locus_tag="VVN06_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU5562111.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17506..18792
                     /locus_tag="VVN06_000508"
     CDS             17506..18792
                     /locus_tag="VVN06_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5562112.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDHDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18794..19663
                     /locus_tag="VVN06_000509"
     CDS             18794..19663
                     /locus_tag="VVN06_000509"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU5562113.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPIIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19733..19813
                     /locus_tag="VVN06_000510"
                     /pseudo
     CDS             19733..19813
                     /locus_tag="VVN06_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20004..20942
                     /gene="msrB"
                     /locus_tag="VVN06_000511"
     CDS             20004..20942
                     /gene="msrB"
                     /locus_tag="VVN06_000511"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5562114.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21000..22724
                     /locus_tag="VVN06_000512"
     CDS             21000..22724
                     /locus_tag="VVN06_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562115.1"
                     /translation="MKYLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22726..24474
                     /locus_tag="VVN06_000513"
     CDS             22726..24474
                     /locus_tag="VVN06_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562116.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRNLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24731..25990
                     /locus_tag="VVN06_000514"
     CDS             24731..25990
                     /locus_tag="VVN06_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU5562117.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26066..26201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26240..26740
                     /locus_tag="VVN06_000515"
     CDS             26240..26740
                     /locus_tag="VVN06_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5562118.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26816..27184
                     /gene="rplL"
                     /locus_tag="VVN06_000516"
     CDS             26816..27184
                     /gene="rplL"
                     /locus_tag="VVN06_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5562119.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT         8071 a         5094 c         6152 g         7949 t
ORIGIN      
        1 atcaaatttt ccagatacct actgaagctg atttatttct tcatctttct tctactacct
       61 ttgtcactaa atttgacaga cacatattgc tgatacaacc tctctaccta taatctcacg
      121 gttaacctga ggctagtttc atagtttgct atttgatttt cgttgataaa aagaaatgct
      181 cctccaagac ggaagagcat tttttattgt gattcttaca catttatttc ctagttttgc
      241 aggaagtcta gtataccatt ttttaaaaga agagtaaaaa agcaagggta gtttcttttt
      301 tgagaattgt ctaaaagttt gatataatgg tagttatgaa tagaataaga gtcagcaaaa
      361 gggttgaaaa gaagcttgct aaggggctag ttttactaga agccagtgat cttgagaatg
      421 tcaatcttaa ggatcaggaa gtagaggtgc agggtcagga aggaaacttt cttgggactg
      481 cctacctttc tcagcaaaac aagggcttgg gctggtttgt cagcaaagac aaggtggcct
      541 tcaatcaaac tttctttgaa acgttgttta gaaaagccaa agaaaagaga aacgcctact
      601 atcaagatga tttgacaact gcctttcgtc tctttaatca agagggagat ggctttgggg
      661 gtctgacagt ggacctttat ggcgactacg ccgtcttttc ttggtataac tcttatgttt
      721 atcagattcg tcagactata tcagaagcct ttagacaggt tttccctgag gttttagggg
      781 cttatgagaa aatccgcttt aagggttttg actatgaatc tgcccatgtt tatggtcaag
      841 aagcacctga ctttttcact gttttagaaa atggtgtcct gtatcaagtc tttatgaatg
      901 atggcttgat gacaggaatt ttcctagacc agcatgaggt tcgcggtagt ttagttgacg
      961 gcttggctat gggtaaatcc ttgctcaata tgttttccta cacagcggct ttttcagtag
     1021 ctgcggccat gggaggagct agccagacaa cttctgttga tctagccaaa cgttcacgag
     1081 aattgtctca agcgcatttt caggcaaatg ggctcagcac agacgagcat cgttttatag
     1141 tcatggatgt ctttgagtat ttcaaatatg ccaaacgcaa agacttgacc tacgatgtga
     1201 ttgtcctaga tccgcctagc tttgctcgga ataaaaaaca aactttctct gtggccaagg
     1261 attatcacaa gttgatttcc cagagtcttg agattttaaa tccgggaggg attatcattg
     1321 ccagtaccaa tgctgccaat gtttcccgtc agaaatttac agaacaaatt gataaaggct
     1381 ttgcaggaag aagttaccag attttaaaca aatatggtct tccagcagat tttgcctata
     1441 ataaaaaaga tgaaagtagt aattacctca aggtgattag tatgaaggtt agtaaatgaa
     1501 attaatcgtt tcagtaatgc caagaagttt agaggaggct caggctctgg gtgccacgag
     1561 gtacctggat gccgacatca ttgaatggcg tgccgactat ctgcctaaag aagcgatttt
     1621 gcaggtagct ccagccattt ttgaaaaatt cgcaggccgt gagttggttt tcacgctacg
     1681 aactcgctcc gaaggtggag aaatcgacct ttctccagaa gagtatatcc atctaatcaa
     1741 ggaagttgcg caattctatc aaccagacta tattgatttt gagtactata gctacaagga
     1801 tgtttttgag gaaatgctgg acttcccaaa tcttgttttg agttaccata atttccaaga
     1861 aacacctgag aacatgatgg aaatcttgtc agagttgacg atcctaaatc caaaacttgt
     1921 taaggttgcg gtgatggctc acacggagca ggatgtctta gacttgatga actatacacg
     1981 aggctttaaa accctcaatc ctgaacagga atatgtgacc atttctatgg gtaaggtggg
     2041 caaggtctct cgtatcactg cggatgtgac tggttcgagt tggtcttttg ccagtctgga
     2101 tgaggttagt gcccctgggc agatttctct agctagcatg aaaaaaatca gggagatttt
     2161 ggatgaagct tgatggctat acacgtttag ctgcagttgt tgccaatcct attaagcatt
     2221 ctatttctcc cttcatccac aatagagcct ttgaggcgac agctaccaac ggtgcttatg
     2281 tggcttggga gattgaagcg agtgacttgg cagaaacagt ggccaatatt cgtcgctacc
     2341 agatgtttgg catcaatctg tccatgccct ataaggagca ggtgattcct tatttggata
     2401 agctgagcga tgaagcgcgc ttgattggtg cggttaatac ggttgtcaat gagaatggca
     2461 atttaattgg atataataca gatggcaagg gattttttaa gtgcttgcct tcttttacaa
     2521 tttcaggtaa aaagatgacc ctgctgggtg caggtggtgc ggctaaatca atcttggcac
     2581 aggctatttt ggatggcgtc agtcagattt cggtctttgt tcgttcagtt tctatggaaa
     2641 aaacaagacc ttacctagac aagttacagg agcaaacagg ctttaaagtg gacttgtatg
     2701 ctttagaaaa tgtttctgaa ctgcaagcaa ggattgtcga gtcggattta ctggtcaatg
     2761 ccaccagtgt gggcatggat ggtcaatcat ccccagttcc tgaaagcatc aatttgccag
     2821 aaactatctt ggtggcagat attatttatc aaccctttga aacgccattt ttaaaatggg
     2881 ccagaagtca gggcaaacca gcggttaatg gtctgggaat gttgctctat caagctgcag
     2941 aagcttttca actgtggaca ggcaaggaaa tgccaacaga agagatttgg cagtctttaa
     3001 cagaaaaata ccaataaaga aaaggagatt ctcctatgaa aatcagaatc gatattcctc
     3061 atcatcctta tgatattcag attgaaaaag gttgtctggc tcaggctggt caatggttgc
     3121 gagaactctg gcaaccacaa aaggtagtca ttgtgacaga taaccatgta gcttctcttt
     3181 atgcagagaa ggtcaagctc agcctagaag atgctggttt tcaggtagct gtttttgact
     3241 ttttagaagg ggaagaaaga aagaatttaa ccactgttca gaaagtctat gaattcctag
     3301 ttaagcaggg gttgactcgt agcgatggaa tcgtggctct cggtggtggt gtcgttgggg
     3361 acttggctgg ttttgtagcc tctacctata tgcggggcat tcattttgtt cagataccga
     3421 ctagcttaac agcccaggtg gattcttcta ttggtggaaa gacgggtgtt aacactccat
     3481 ttgccaagaa tatggtagga acttttgccc aaccagatgg ggttttgatt gacccacttg
     3541 ttcttgaaac gcttggaaaa agagagttga ttgaagggat gggtgaggtt atcaagtatg
     3601 gcttgattga ggatccagaa ctgtgggctc ttttgacgga gctggatggt tctgttgaga
     3661 gcattctgga acatgcagag accttgattg aacattcttg tcaggttaag cgcaagatgg
     3721 tggttgagga tgagttggac aatggtgtac gcctttacct caactttggt cacactattg
     3781 gtcatgctat tgaagcaact gccggttatg gtaaggtcat gcatggtgag gctgtggcca
     3841 tgggcatggt gcagatttcc aaggttgctg agaaaaaagg cctcatgcca gctggcataa
     3901 ctcagtccat tacagatatg tgtcagaaat ttggcttgcc tgttgactat gaaaactggg
     3961 atgttgacaa gctttatcag gctttgactc atgacaaaaa agcgcgtggc aataccttga
     4021 aattggtctt ggtaccagag cttggttcag cgaccattca cccagtttct ctggaagaga
     4081 tgaaagacta cttggtaaaa taaggagagt gtatgagata tttaactgca ggagaatcac
     4141 acggtccccg tctgacagct atcattgaag gaattccagc tggactacct ttgacagctg
     4201 aggatatcaa tggcgacctt aaacgccgtc agggtggcta cggtcgtggc ggtcgtatga
     4261 agattgagag cgaccaagtt gtctttactt caggtgttcg ccatgggaag acgacagggg
     4321 ctcctattac catggatgtc gtcaataagg accaccaaaa atggctggat atcatgtctg
     4381 cagaggacat tgaagaccgc cttaaaagca aacgaaaaat cacccatcct cgcccaggac
     4441 atgccgattt ggttgggggc atcaagtacc gttttgatga tttgcgaaat tctttggagc
     4501 gttcatcagc tcgtgaaacc accatgcggg tggcagttgg tgcagtagcc aaacgcctct
     4561 tggctgagct ggatatggag attgccaacc atgtcgtggt ctttggtggc aaggaaatcg
     4621 atgttcctga aaatctgaca gtcgctgaaa ttaagcaaag agctgcccag tctgaagttt
     4681 ctattgttaa ccaagaacga gaacaggaaa tcaaggacta tattgaccaa atcaaacgtg
     4741 atggtgatac catcggtggg gttgtggaga cagtcgtcgg aggcgttcca gttggtcttg
     4801 gttcctatgt ccaatgggat agaaaattgg atgcaagatt ggctcaagct gttgtctcta
     4861 tcaatgcctt taaaggggtg gaatttggtc ttggctttga ggctggttat cgtaaaggca
     4921 gccaagttat ggatgaaatt ctctggtcta aagaagacgg ttatactcgc cgtaccaata
     4981 atctaggtgg ttttgaaggt ggtatgacta atgggcaacc catcgttgtt cgtggggtca
     5041 tgaaacccat tcctactctt tataaacctc ttatgagtgt ggatatcgaa acccacgaac
     5101 cttacaaggc aaccgtggag agaagtgatc cgactgctct tccagctgca ggaatggtca
     5161 tggaagcagt tgtagcaacg gttctggcgc aagaaatcct cgaaaaattc tcatcagata
     5221 atcttgagga actaaaagaa gcggtagcca aacaccgaga ctatacaaag aactattaag
     5281 gagttcctat ggcaaaaaca atctatatcg caggtcttgg gttgattgga gcctctatgg
     5341 cacttggtat caaacgcgat catccagatt atgaaatttt aggttataat cgtagtcaag
     5401 cttcgagaga tatcgccttg aaagaaggca tgattgaccg tgcaacggat gattttgcta
     5461 gttttgctcc tttggcagat gtcattatac tcagcttgcc aatcaaacaa actattgctt
     5521 tcattaagga gttggccaat ttggatttgc gagaaggcgt tattatttca gatgctggtt
     5581 cgaccaagtc aaccattgtg gatgcggcgg agcagtattt ggctggcaag tctgttcgct
     5641 ttgtcggggc ccatcccatg gctggtagtc acaagacagg ggctgcttcg gcagatgtca
     5701 atctttttga aaatgcctat tatatcttta caccttcaag cctgacaagt caggacacgc
     5761 ttaaggaaat gaaggatctg ctttcaggtc ttcatgctcg ttttatcgag attgatgcca
     5821 aggagcatga tcgtgtcact tctcagatta gccattttcc tcatattttg gcttctagtc
     5881 tcatggagca gactgcggtc tatgctcaag agcatgagat ggcaaggcgc tttgcggcag
     5941 gtggttttcg agatatgacc cgaattgcgg aaagcgagcc aggaatgtgg acctccattc
     6001 tcttgtccaa tagcgagacc attctggata gaattgagga tttcaaggaa cgtttggaag
     6061 tgattggtca ggccattagt aagggagatg aagagcaaat ttggaacttt tttaaccaag
     6121 cgcgtgaaca acgccagacc atggaaatcc ataagcgtgg tggtgtggat agctcttacg
     6181 acctctatgt tgacgttcct gatgaagaag atgtcatcct gcggattttg gaactgctac
     6241 gtggaacttc cttggttaac atccacatca acgaggaaaa tcgtgaggat attcacggaa
     6301 tcctacaaat ttcatttaaa aatgctcaag acttggaaag agctgagcat ctcataacag
     6361 aaaataccga ctatacagtc gtcatcaaat aaggagaaga acatgtcaaa tatttacgat
     6421 agtgcaaacg aactcagtcg cggtctacgc ggattaccag aatacaagac tgttaaagca
     6481 gctaaagatg cgattgcagc agatgctgag gcaagcaaaa tctttacaga atatcttgcc
     6541 tttcaagagg aaattcaaaa actagcacag acaggtcaaa tgccagatgc gtccttccaa
     6601 gctaagatgg aaggctttgg taaacagatt caaggaaata gcctcttgtc agaattcttt
     6661 accaagcaac aacaattggc catttacctt tctgacattg aaaaaattgt ttttgaacca
     6721 gtttcagaat tgctgaaata agaaaagaac gatcctaaag tcaggaattt ttaaggaatt
     6781 tctggctttt tatgataaaa taggtaaaag tattgcaagt atgaggtcca gtatgaaact
     6841 aaaaacaaac attcgccatt tacatggtag tatccgcgtc ccaggtgaca agtctatcag
     6901 ccaccgttcc attatctttg gaagtttggc tgagggtgag accaaggttt atgatattct
     6961 gcgaggtgaa gacgtccttt cgaccatgca ggtttttcgt gaccttggtg ttgaaattga
     7021 ggataaagat ggggttatta ccattcaagg tgtaggcatg gctggcttaa aagcgccaca
     7081 aaatgccctt aatatgggaa attctggcac ctcgattcgc ctgatttcag gtgtccttgc
     7141 tggtgcagat ttcgaagtag agatgtttgg agatgatagt ctttccaaac gtcctatgga
     7201 ccgtgtgacc cttccactga aaaaaatggg cgtcagcatt tcaggacaaa ctgaacgaga
     7261 cttgcctccc cttcgcttaa aagggacgaa aaacctaaga cctattcatt atgagttgcc
     7321 aattgcctct gcccaagtca agtcagcctt gatgtttgca gccttacaag ctaagggggc
     7381 gtcagttatt atcgaaaaag agtgcactcg taatcatact gaagatatgt tgaaacaatt
     7441 tggtggtcat ttaagtgtgg acggtaagaa aatcatagtc caaggaccac aaaaattgac
     7501 aggacagaag gtggtcgtac caggagatat ttccagtgca gccttttggt tagtcgcagg
     7561 tttgattgtt ccaaattctc gtctagtgct gcagaatgtg gggatcaacg aaactcgcac
     7621 cggtattatt gatgtcattc gtgccatggg tggaaaattg gaaataactg aaatcgatcc
     7681 agtcgctaaa tctgcaacct tgattgttga gtcttctgac ttgaaaggaa cagagatttg
     7741 tggcgctttg attccacgtt tgattgatga attgcctatt attgccctac ttgcgaccca
     7801 agcccaaggt gtaacagtta tcaaggatgc tgaggagctc aaggtcaagg aaacagaccg
     7861 tattcaggtt gtggcagacg ccttaaatag tatgggagca gatattactc ctacggcaga
     7921 tgggatgatt atcaaaggaa aatcagctct tcacggtgct agagtcaata cgtttggtga
     7981 ccaccgtatc ggcatgatga cagctatcgc agccctcttg gttgcagatg gagaggtgga
     8041 gcttgaccgt gcagaagcca tcaataccag ctatcctagt ttctttgatg atttggagag
     8101 cttgattcat ggctaaggtg ttattagggt ttatgggggc tggaaaatcg actattgcaa
     8161 gaggcttgga cactaattac cttgatatgg atgctctgat tgagaagcgc ctaggtatgt
     8221 ccattgcgaa ttttttcgct gaaaagggag aagcagcctt tcgtcatgta gaatcagaag
     8281 tcctagctga tttactacaa acagaccaag tcgtgtcaac tggaggagga gtggttattt
     8341 ctcagagaaa tcgtgactta ctcaagacta atacagataa catctacctg aaagcagatt
     8401 ttgaaaccct ctacctacgt atcgcagctg ataaggacaa tcagcgaccg ctttttctaa
     8461 ataatagcaa ggaagaacta gtagctattt ttcaagaaag acaggcttgg tatgaggaag
     8521 tggctagtcg ggttttggat gtgaccaagc taagcccaga ggaaattata gaggaactaa
     8581 gatgaaaatt gcttatctag gtcccaaggg atcattttca caccacgttg tgcagacagc
     8641 ttttcctcat gaggaattgc aggcttttgc caacattaca gatgtcatca aggcttatga
     8701 gcaaggatta gtggactatt ctgtggtgcc agttgaaaac tctattgagg gtagtgttca
     8761 tgaaaccttg gactatcttt ttcatcaggc tcacatccaa gcagtagcag aaatcgttca
     8821 gcctattcat cagcagttga tggtggttcc aggccatact aagattgaaa agattttttc
     8881 acatccacag gccttggctc aaggaaagaa attcatcgat gaacaatatc cagaggctca
     8941 aatcgaggta acagctagta cagcttatgc ggcccgtttt atttccgaac atccagatca
     9001 gccctttgca gcaattgcac ctagaagttc tactgaagaa tatggattgg aactgattgc
     9061 cgaggatatt caggaaatgg aagccaattt aacacgtttc tgggttctag gagctgaaaa
     9121 gcctagtatt cccttgcaag cacaaactga aaagatgagt ttggccttga cattacctga
     9181 caaccttcca ggtgcacttt ataaggccct gtcgaccttt gcttggcgag gaattgactt
     9241 gacaaaaatt gaaagtcgtc cactcaagac agcactgggt gaatactttt tcattatcga
     9301 tgtggattat accgataagg acttggtcca ctttgcccaa aaagaattag aagcgattgg
     9361 aatccagtat aaaattctgg gtgcctatcc tatttatcca atatcagacc atggaaagga
     9421 gagaagatga gtaaagaaaa tcccttaagt catcatgagc agttgcgtta tgattatttg
     9481 ctaaaaaata ttcactatct caatgagaga gaaaaaaatg agtttgccta tttgcaagaa
     9541 aagctaactc ttgctagggg aaatagtagc tctagcttgg aacaagaaag agaagagcag
     9601 gttgacttac caagctatgc gaaccggagt cgctcacaat ccaaatcaca agcactctct
     9661 ttccctccaa aaaagaaaag acggaagctc cgtcttaaac gaatttttat ggtgattttt
     9721 tcacttttgg tctgtgtggc tttggccatg gtattcatgt ttttgcgtgg ttaccaagat
     9781 gctagcgcaa agaaaactgc tgatgcccgg gcagctcaag tagaagtctt taatggtcag
     9841 gacactagag atggagttaa tattttaatc atgggtactg atggtcgaat cggccagaac
     9901 agtgttgaga cacgaactga ctctattatg gtattaaatg tcgggggctc agataagaaa
     9961 atgaagctag tcagtttcat gcgtgataat ttggtctata tagatggtta tagtcaagtg
    10021 attaacggta gaaaacagac agataacaag ttaaacgtag cctacgagtt aggagaacaa
    10081 gaggggcaaa aaggggcaga aatggttcgc caagtcttga aagataattt tgacttggac
    10141 attaagtact acgccttggt tgatttccag gcctttgcca cagcgattga cacgcttttc
    10201 cctgatgggg tgacgattga tgctcaattt tcaacattga atgggcgtcc cctaacagaa
    10261 gctacagtcg gagatgattt atacgctacc gagactgagt ctccaaccca aaccattaaa
    10321 gtcggaaaac agcagatgaa tggctcgacc ctgctcaatt atgctcgttt ccgtgatgat
    10381 gatgaggcgg attacggccg tacaaaaaga cagcaacaag ttttaacagc aattttagag
    10441 caaattaaag atcccactaa acttttcact ggttctgaag ctcttggaaa ggtttttgct
    10501 atgacctcaa ccaacgtccc ctatactttc ctcctaacaa acggtttatc tgttttggat
    10561 ggagcaaaaa atggtattga aaaattgacg attccagaac tgggtgactg ggtagatgcc
    10621 tatgatgttt atggaggctt gggcttgctg gttgatcaaa acaaatatca aaccaagctc
    10681 gctcaaatgg gcttgagata agataatact caatgaaaat taaagtgcaa actaggaagc
    10741 tagccgcagg ctgctaaaaa cacagttttg aggttgtaga taagactgac gaagtcagtt
    10801 acctatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtataag
    10861 acagaaaaat caaagtttga tagctatttg gatagcagtc aggctttgat tttttacagt
    10921 ctgcaataga ttttcacccc ttgtttgtgg tataatgaaa cgatacgata gaaagaataa
    10981 tgaacaatat gactgattta aaagcaattc aggctcgtag tctggagatg gctgaatatt
    11041 ttgtggcctt ttgtaaagaa catgatttgc tctgttatct ctgtggcgga ggcgctattg
    11101 gtgcccttcg aaacaaggga tttattcctt gggacgacga cctagacttt tttatgcctc
    11161 gtaaagatta tgagaaatta gcagaattat ggcctcgtta tgcagatgaa cgttatttct
    11221 tgtcaaagag tcataagaat tttgttgatc gtaatctttt tattaccatt cgtgacaagg
    11281 aaaccacctg tatcaagcct tatcagcagg atttggattt gccacatggt ctggccttgg
    11341 atattttgcc tttggattat tatccgaaaa atccagctga gcggaaaaaa caggttcgtt
    11401 gggccttgat ttattcactc ttttgtgcgc aaactattcc agaaaagcat ggtgctctca
    11461 tgaaatgggg aagtcgcatt ttactgggtt tgactccaaa atctctccgt tatcgcatct
    11521 ggaaaaaagc tgagaaagaa atgactaagt atgatttggc tgattgtgat ggcattacag
    11581 aattatgctc aggtcctggc tacatgagaa acaagtaccc aatcacatct tttgaagaca
    11641 atcttttctt gccatttgaa ggaacagaga tgcctattcc aatcggctat gatgtctatc
    11701 tcagaactgc ttttggggat tatatgacgc ctccaccagc agacaagcag gtaccgcatc
    11761 atgatactgt cattgctgat atggataagt cttatacaaa atacaaggga gaatatggtg
    11821 gctaagaaaa aaatcttatt ttttatgtgg tctttttctc ttggaggtgg tgcagagaag
    11881 attctatcaa ccattgtttc aaatctggat ccagaaaagt atgatattga tattcttgaa
    11941 atggagcact ttgacaaggg atatgaatct gttccaaagc atgtacgcat tttaaaatcc
    12001 cttcaagatt atcgccaaac cagatggtta cgagcttttt tgtggagaat gagaatttat
    12061 tttccaagac tgactcgtcg tttgcttgta aaagatgatt atgatgttga agtttctttt
    12121 accattatga atccaccact gttgttctct aaaagaagag aagtcaagaa gatatcttgg
    12181 attcatggaa gtattgaaga acttcttaag gatagctcta aaagagaatc acatagaagc
    12241 cagttggatg ctgcgaatac aattgtaggg atttcaaaaa agaccaggaa ttctatcaag
    12301 gaagtttatc cagattatgc ttctaaatta cagacaatct acaatggata tgattttcag
    12361 actattctag aaaaatctca agagaagatc gatatcgaga ttgctcctca aagtatctgt
    12421 actatcggac ggattgagga aaataagggt tctgaccgtg tagtggaagt gatacgatta
    12481 ttacaccaag agggaaaaaa ctatcatctc tattttatcg gggctggtga tatggaagag
    12541 gaactgaaaa aacgagtcaa agagtatggg attgaggact atgtacattt ccttggttat
    12601 caaaaaaatc cttatcagta tctatctcag acgaaagttc tcttgtctat gtctaaacaa
    12661 gaaggttttc ctggagtgta tgtggaggcc ttgagtctgg gactcccttt tatctctacg
    12721 gacgttggag gggctgagga attatcccaa gaaggacgat ttggacaaat cattgagagc
    12781 aatcaagagg cagctcaggc gattactaat tacatgactt ctgcctcaaa ctttgatgtc
    12841 gatgaggcta gccaattcat tcaacaattt acaattacaa aacaaatcga acaagtagaa
    12901 aaactattag aggagtagca tggaaactgc attaattagt gtgattgtgc cagtctataa
    12961 tgtggcgcag tacctagaaa aatcgatagc ttccattcag aagcagacct atcaaaatct
    13021 ggaaattatt cttgttgatg atggtgcaac agatgaaagt ggtcgcttgt gtgattcaat
    13081 cgctgaacaa gatgacaggg tgtcagtgct tcataaaaag aacgaaggat tgtcgcaagc
    13141 acgaaatgat gggatgaagc aggctcacgg ggattatctg atttttattg actcagatga
    13201 ttatatccat ccagaaatga ttcagagctt atatgagcaa ttagttcaag aagatgcgga
    13261 tgtttcaagc tgtggtgtca tgaatgtcta tgctaatgat gaaagcccac agtcagccaa
    13321 tcaggatgac tattttgtct gtgattctca aacatttcta aaggaatacc tcataggtga
    13381 aaaaatacct gggacgattt gcaataagct aatcaagaga cagattgcaa ctgccctatc
    13441 ctttcctaag gggttgattt acgaagatgc ctattaccat tttgatttaa tcaagttggc
    13501 caagaagtat gtggttaata ctaaacccta ttattactat ttccatagag gggatagtat
    13561 tacgaccaaa ccctatgcag agaaggattt agcctatatt gatatctacc aaaagtttta
    13621 taatgaagtt gtgaaaaact atcctgactt gaaagaggtc gcttttttca gattggccta
    13681 tgcccacttc tttattctgg ataagatgtt gctagatgat cagtataaac agtttgaagc
    13741 ctattctcag attcatcgtt ttttaaaagg ccatgccttt gctatttcta ggaatccaat
    13801 tttccgtaag gggagaagaa ttagtgcttt ggccctattc ataaatattt ccttatatcg
    13861 attcttatta ctgaaaaata ttgaaaaatc taaaaaatta cattagaacg ggggtgacca
    13921 agtgattgat gggaaacgat tattatttag tttgaccata gtcagttatg ccttgacgct
    13981 agtaagtgga attgtgtatc tgtttaataa taataatgtt agcttacttt ctactttatt
    14041 gttcttgttg gttagtagct taattgcttg ttggaatgat atcaagtatt acttaatcca
    14101 ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca ttgattattt
    14161 tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct ttatagttgt
    14221 catggtttcg attctgggct tgaccacagg aggcattctg gctcgttact tcatagctag
    14281 gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata tcaagcggtt
    14341 acgctttgta tcactagtag tttttcttct aacctatcct ttctatttca ttcggttatt
    14401 tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa attttgaaag
    14461 taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa tgtgtatgta
    14521 tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct ttattgcagc
    14581 taatactatt catttggcaa ttgggacacg aaatcccttt attttaagta ttttatttgc
    14641 ttttgtttat tactttatgc gggagcaaac tgaaaaagga aaatggattg ggtttaaaga
    14701 aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag tactcaatta
    14761 tgtacgggat aatgtccaag tttcccatac aggtttctgg gatatcttac ttgactttat
    14821 ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta acagtagcct
    14881 cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt ttgcaagggg
    14941 gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata gtgtggaact
    15001 agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga acaaggaata
    15061 cttgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg actatggtat
    15121 gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca tgctgcaagt
    15181 agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga ataatctatt
    15241 ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa tgcaattctg
    15301 gggaattgtt cttgtgatta tatttgtagc aaaaatgctt acaaaggaaa actagtatct
    15361 actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat ttgtttgcaa
    15421 atctttataa aaagcgaaaa gcaaatattt tagctctcat cgctttattt gctctcattg
    15481 ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat acaacaagtt
    15541 attcaactta tcttatctat aaaatcactc ctccagaaga ttcggccaaa acgattttga
    15601 atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt aatttgaatg
    15661 gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc agtgaattag
    15721 acacgacaga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa aaattgaccg
    15781 tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca agtaaagatg
    15841 ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa catgcttatg
    15901 caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac gcaaatggtg
    15961 aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt attggagtgg
    16021 tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat ccaacaatca
    16081 atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt acaacaattg
    16141 ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc atcgttagct
    16201 caaataaagc tatcctagat aaattgaaac agagtcaaga agctttaaaa ggaatgcatt
    16261 ttgtagattt acaggatgta tcatctcttt tggaaagaga tacagtcctt cttgttgaag
    16321 agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc agaaacttaa
    16381 atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt atagggtctt
    16441 caaagtcggc taagagccga ttttgaaggc tttttctatc tgtctgcaga aattttctct
    16501 tggagtctcc tatagaaaga ccctagggaa aatctatccg tatagaggag ccttcttttg
    16561 ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt actagcaatg
    16621 aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga ggatttgatg
    16681 gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga agagttcttt
    16741 tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg catgttgagc
    16801 ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc aaagattgac
    16861 caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt ggctcaaatg
    16921 tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac caaggatgat
    16981 atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca agagcaggct
    17041 gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc ttttgctaag
    17101 ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac ttctgaactc
    17161 tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa tcatccaagc
    17221 ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag tgatatcagt
    17281 cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt gctcaatctg
    17341 caaaaaatcg gctaatcctt tctggaaagc tattttcaga ttttaagaag agcgaatcgt
    17401 ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga attttcacaa
    17461 aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac agttaaaatt
    17521 gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa ggaaaatgga
    17581 ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt ggtcaaggat
    17641 gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt aaccaatgtg
    17701 gatgatattt tatcagacca tgatattact atcgtagtgg aattgatggg gcgtattgag
    17761 cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt tactgctaac
    17821 aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc taacaaggta
    17881 gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac tttagcaaat
    17941 tccttggctt ctgataaaat tacgcgcgtg cttggagtag tcaacggaac ttccaacttc
    18001 atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc ggaagcacaa
    18061 cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga tgcagcctac
    18121 aagatggtta ttttgagtca atttgccttt ggcatgaaga ttgcctttga tgatgtagcc
    18181 cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga gcttggttac
    18241 gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc agaagtgact
    18301 ccaaccttcc tacctaaagc gcacccactt gctagtgtga atggcgtaat gaacgctgtc
    18361 tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc gggtcaaaaa
    18421 ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt gaatgatggt
    18481 actattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa tcctgaagat
    18541 gtcaaagcaa actactattt ctcaatcttg gctctagact caaaaggtca ggtcttgaag
    18601 ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca agatggcaaa
    18661 gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc ccagcttgaa
    18721 aatgtctcag ctgaattgaa gaaggtttca gaattcgacc tcttgaatac cttcaaggtg
    18781 ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg ggccaggttt
    18841 tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg aagaacgaga
    18901 tgagtggctg attgaacacc agattggcaa atggattcca catgatgagc gtaatctctt
    18961 gctcaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga aaatgaccag
    19021 tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg ctgggattga
    19081 actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc agttagcgac
    19141 caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc tcgttattgc
    19201 aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt gtgattttct
    19261 agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct tgcctaaaaa
    19321 attgtcttat aaggaagctg ttgctgcaag ttctatcgcc aatgtagcgg ttgctgcctt
    19381 gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct tccatgagcg
    19441 ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca aagaaaatgg
    19501 ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg cttctcatga
    19561 caagatgcca ataattaagg cagaattgga aaagcaacct ttcaaaggaa aactgcatga
    19621 cttgagagtt gatacccaag gtgtccgtgt agaagcaaaa taaagaatag aatataggat
    19681 ggggaaactc ttgaccagag gggttcatat cctttttgtg aaaagaagtt tatactcaat
    19741 gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc tcaaagcact gctttgaggt
    19801 tgtagataag actgacgacg tcagctcaaa acagtgtttt gaggttgtgg atagaactga
    19861 cgacgtcagc tcaagacact gttttgaggt tgcagataga actgacgaag tcagtaacca
    19921 tacctacggt aaggtgacgc tgacgtggtt tgaagagatt ttcgaagagt attagttaaa
    19981 aacgtgataa aggagaaata aaaatggcag aaatttatct agcaggtggt tgtttttggg
    20041 gcctagagga atatttttca cgcatttctg gagtgctaga aaccagtgtt ggctacgcta
    20101 atggtcaagt cgaaacgacc aattaccagt tgctcaagga aacagaccat gcagaaacgg
    20161 tccaagtgat ttacgatgag aaggaagtgt cactcagaga gattttactt tattatttcc
    20221 gagttatcga tcctttatct atcaatcaac aagggaatga ccgtggtcgc caatatcgaa
    20281 ctgggattta ttatcaggat gaagcagatt tgccagctat ctacacagtg gtacaagagc
    20341 aggaacgcat gctgggtcga aagattgcag tagaagtgga gcaattacgc cactacattc
    20401 tggctgaaga ctaccaccaa gattatctca ggaagaatcc ttcaggttac tgtcatatcg
    20461 atgtgaccga tgctgataag ccattgattg atgcagcaaa ctatgaaaag cctagtcaag
    20521 aggtgttgaa ggccagtcta tctgaagagt cttatcgtgt cacacaagaa gctgctacag
    20581 aggctccatt taccaatgcc tatgaccaaa cctttgaaga ggggatttat gtagatatta
    20641 cgacaggtga gccactcttt tttgccaagg ataagtttgc ttcaggttgt ggttggccaa
    20701 gttttagccg tccgatttcc aaagagttga ttcattatta caaggatctg agccatggaa
    20761 tggagcgaat tgaagttcgt tctcgttcag gcagtgctca cttgggtcat gttttcacag
    20821 atggaccgcg ggagttaggc ggcctccgtt actgtatcaa ttctgcttct ttacgctttg
    20881 tggccaagga tgagatggaa aaagcaggat atggctatct attgccttac ttaaacaaat
    20941 aaaacagaga gtggggcttc ccactttctt catttctaga atatgaatag aagggattta
    21001 tgaaatacct attatcttac ttcaaaccct acatcaagga atcaatttta gcccccttgt
    21061 tcaagctgtt agaagctgtt tttgagctct tggttcccat ggtgattgct gggattgttg
    21121 accaatcttt acctcaggga gatcaaggtc atctctggat gcagattggc ctgctcctta
    21181 tctttgcagt aattggcgtt ttagtggcct tgatagctca attttactca gcaaaggcag
    21241 cagtaggctt tgctaaggaa ttgacaaacg atctttatcg tcatattctt tccttgccca
    21301 aggacagcag agaccgtctg acaacttcta gtttggtcac tcgcttgact tcggatacct
    21361 accagattca gactggtatc aatcaattcc tgcgtctctt tttgcgagcg cccattatcg
    21421 tttttggtgc catttttatg gcttatcgaa tctcagctga gttgactttc tggttcttag
    21481 tcatggttgc cattttgacc attgtaattg tagggttatc tcgattggtc aatcctctct
    21541 acagtagtct cagaaagaaa acggaccaac tggttcagga aacgcgccag caattgcaag
    21601 ggatgcgggt tattcgtgct tttggtcaag aaaaacgaga gttacagatt tttcaaaccc
    21661 ttaaccaagt ttatgctaga ttacaagaaa agacaggttt ctggtctagt ttattaacac
    21721 ctctgaccta tctgattgtc aatggaactc ttctcgttat tatctggcaa ggctatattt
    21781 caattcaagg aggagtgctc agtcaaggtg ctctcattgc tcttatcaat tacctcttac
    21841 agattttggt ggaattggtc aagctagcca tgttgatcaa ttccctcaac cagtcctata
    21901 tctcagccaa gcgaatcgag gaagtctttg ttgaggctcc agaggatatc cattcagagt
    21961 tagaacaaaa gcaagctacc agagataagg ttttacaagt ccaagaattg acctttacct
    22021 atcctgatgc ggcccagcct tctctgagag acatttcctt tgatatgact caaggacaaa
    22081 ttctaggtat catcggggga actggttctg gtaaatcaag cttgattcaa ctcatacttg
    22141 gactttatcc agtaaacaag ggaagaattg acctttatct agatggattg agtcctctta
    22201 atttggagca atggagatct tggattgcct atgtgccaca aaaggtcgaa ctctttaagg
    22261 gaactattcg ttccaacttg actctaggtt tcaatcaaga agtatctgac caggaactct
    22321 ggcaggcctt ggagattgcg caagctaagg attttgtcag tgaaaaggaa ggactcttgg
    22381 atgctctagt tgaggcaggg gggcgaaatt tctcaggtgg acaaaaacaa agattgtcta
    22441 tcgcccgagc agtcttgcgc caggctccgt ttctcatcct agatgatgca acctcggcac
    22501 tggataccat tacagagtcc aagctcttga aagctattag agaaaatttt ccaaacacga
    22561 gcttaatttt gatctctcaa cgaacctcaa ctttacagat ggcggaccag attctcctct
    22621 tggaaaaagg tgagttgcta gctgttggca agcacgatga cttgatgaaa tccagccaag
    22681 tctattgtga aatcaatgca tcccaacatg gaaaggagga ctagaatgaa acgacaaact
    22741 gtaaaccaga cgctcaaacg tttagccgta gatttagcaa gccatccttt cctccttttc
    22801 ctagcctttc taggaactat tgcccaagtt ggcttatcaa tttacctacc tattctgatt
    22861 gggcaggtca ttgaccaagt cctagtggct ggttcatcac cagttttttg gcagattttt
    22921 ctccagatgc tcttggtggt aataggaaat actctggtac aatgggccaa tcctctcctc
    22981 tataatcgtc taatcttctc ttataccaga aatttacgag agcgaatcat ccataaactc
    23041 catcggttac cgattgcttt tgcggatcgg caaggcaatg gagagatggt cagtcgtgta
    23101 accacggata tcgaacagtt ggcagctggc ttgaccatga tttttaacca atttttcatt
    23161 ggtgttttga tgattttggt tagtattcta gccatgctcc aaattcatct cctcatgact
    23221 ctcttagtct tgctgttgac gccactgtcc atggtgattt cacgctttat tgccaagaaa
    23281 tcctatcatc tcttccagaa gcaaacagag acgaggggaa ttcagactca gttgattgaa
    23341 gaatcgctta gtcagcagac tataatccag tccttcaatg ctcaaacaga atttatccaa
    23401 agattgcgtg aggctcatga caactactca ggctattctc agtcagccat cttttattct
    23461 tcaacggtca atccttcgac tcgctttgta aatgcactca tttatgccct tttagctgga
    23521 gtaggagctt atcgtatcat gatgggttca gccttgaccg tcggtcgttt agtgactttt
    23581 ttgaactatg ttcagcaata caccaagccc tttaacgata tttcttcagt gctagctgag
    23641 ttgcaaagtg ctctggcttg cgtagagcgt atctatggag tcttagatag ccctgaagtg
    23701 gctgaaacag gtaaggaagt cttgaccagt gaccaagtta agggagctat ttcctttaaa
    23761 catgtctctt ttggctacca tcctgaaaaa attttgatta aggacttgtc tatcgatatt
    23821 ccagctggta gtaaggtagc catcgttggt cctacaggtg ctggaaaatc aactcttatc
    23881 aatctcctta tgcgttttta tcccattagc tcgggagata tcttgctgga tgggcaatcc
    23941 atttatgatt atacacgagt atcattgaga cagcagtttg gtatggtgct tcaagaaacc
    24001 tggctcacac aagggaccat tcatgataat attgcctttg gcaatcctga agccagtcga
    24061 gagcaagtaa ttgctgctgc caaagcagct aatgcagact ttttcatcca acagttgcca
    24121 cagggatacg ataccaagtt ggaaaatgct ggagaatctc tctctgtcgg ccaagctcag
    24181 ctcttgacca tagcccgagt ctttctggct attccaaaga ttcttatctt agacgaggca
    24241 acttcttcca ttgatacacg gacagaagtg ctggtacagg atgcctttgc aaaactcatg
    24301 aagggccgca caagcttcat cattgctcac cgtttgtcaa ccattcagga tgcggattta
    24361 attcttgtct tagtagatgg tgatattgtt gaatatggta accatcaaga actcatggat
    24421 agaaagggta agtattacca aatgcaaaaa gctgcggctt ttagttctga ataagccatt
    24481 ctcttttgaa agtttatgga cgaaaaaagt tgccttcgag tgactttttt gttacaatag
    24541 ctagaaaaat tgtttactgt aatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    24601 cgtaggttgc tcaaagcaca gctttgaggt tgtagataga actgacgaag tcagtaacca
    24661 cacctacggc aaagtgaatc tgaagtggtt tgaagagagt aaaacttgtc ttttagaaaa
    24721 ggagcctata atgaaagtct ttcagcatgt aaatatcgtg acttgtgatc aagatttcca
    24781 tgtttatctt gatggaatct tagcagtcaa ggattctcaa atcgtctatg tcggtcaaga
    24841 taagccagcg tttttagagc aagctgagca gattatagac tatcagggag cttggattat
    24901 gcctggtttg gtcaattgtc acacccattc tgcaatgaca ggtctgagag ggatccgaga
    24961 tgacagcaat ctccatgaat ggctcaatga ctatatctgg ccagcagaat ctgagtttac
    25021 tcccgacatg actaccaatg cggtcaaaga agccctgaca gagatgctcc agtcaggaac
    25081 aacaaccttt aacgatatgt ataatcccaa tggtgtggat attcagcaaa tttatcaggt
    25141 ggtgaaaact tccaagatgc gttgttattt ttctccgact ctcttttctt cagagacaga
    25201 aacaactgct gagactataa gcagaactcg atccatcata gacgaaatct taaaatataa
    25261 aaatccaaat ttcaaggtta tggtagcacc tcattctccg tatagctgca gtagagactt
    25321 gctggaagcg agtttggaaa tggcaaaaga gctaaatatt ccgctccatg tccatgtggc
    25381 ggagaccaag gaggagtcag gaattatcct caaacgatac ggcaaacgcc cccttgcttt
    25441 tcttgaagaa ctgggttatt tagatcatcc gtccgtattt gctcacgggg tcgaattaaa
    25501 cgagagagaa attgaacgct tggcatcttc tcaagtggct atcgcccaca atcctatcag
    25561 taacctcaaa ctggcatcag gaattgctcc aattatccag ctccaaaaag cgggagtagc
    25621 agtcggaatt gcgactgact cggttgcttc caataacaat ctagatatgt ttgaggaagg
    25681 aaggactgca gctcttcttc agaagatgaa aagtggggat gccagccagt ttccaatcga
    25741 aacagctctc aaggtactga caatcgaagg ggctaaggcc cttggaatgg aaaatcagat
    25801 aggaagtctg gaagtcggca agcaagcaga ttttctggtc attcaaccac aagggaaaat
    25861 tcatctccaa cctcaggaaa atatgctgtc tcacctggtt tatgcagtta aatctagtga
    25921 tgtagatgat gtttatatcg ccggagaaca ggttgttaag caaggtcaag tcctgacagt
    25981 agaactttaa aagaaaaatc acgaaaaatt ttaaaaaaag tttgcaaaaa tcttgcattc
    26041 tttttttgac tatgctatac ttatatacgg tttaaaaaaa ctgcctaaga cagtagggga
    26101 gctcgactca taaatatcct accgaggaca agacgtatca tgtaaaaaga agcgtattgt
    26161 actttcgtgt ctaggtttgg gcgcgttttt ctttttgaaa aattccccaa gcaaaataat
    26221 tacggaggtg aacacactaa tgagtgaagc aattattgct aaaaaagcgg aactagttga
    26281 cgtagtagct gaaaaaatga aagctgctgc atctatcgtc gttgtagacg ctcgtggttt
    26341 gacagttgag caagatacag ttcttcgtcg tgagcttcgt ggaagcgaag ttgagtataa
    26401 agttattaaa aactcaatct tgcgtcgtgc agctgaaaaa gctggtcttg aagatcttgc
    26461 atctgtattt gttggaccat ctgcagtagc attttctaat gaagatgtta tcgcaccagc
    26521 gaaaatcttg aacgactttt ctaaaaacgc tgaagcactt gaaattaaag gtggtgcaat
    26581 cgaaggcgct gtcgcatcta aagaagagat tcttgcactt gcaactcttc caaaccgcga
    26641 aggacttctt tctatgctcc tttctgtact tcaagcgcca gtgcgcaacg ttgctcttgc
    26701 agtcaaagcg gttgcagaaa gcaaagaaga cgcggcttaa tcttaagcta catagcgtag
    26761 cctagctacg aaaaaaacta ttataaaatt taaaacttat ttggaggaaa taacaatggc
    26821 attgaacatt gaaaacatta ttgctgaaat taaagaagct tcaatccttg aattgaacga
    26881 ccttgtaaaa gctatcgaag aagaatttgg tgtaactgca gctgctcctg tagctgttgc
    26941 tgcagctgat gcagctgatg ctggtgctgc taaagattca ttcgacgttg aattgacatc
    27001 tgcaggcgac aaaaaagttg gtgttatcaa agttgtacgt gaaatcactg gtcttggtct
    27061 taaagaagct aaagaacttg ttgacggtgc accagcactt gttaaagaag gcgttgcaac
    27121 tgcagaagct gaagaaatca aagctaaatt ggaagaagct ggagcttcag ttactcttaa
    27181 ataatagagc aactacatta gtagcttaaa aacatgatta aaccgctatt cttaggagta
    27241 gcggtttttc tttttgttta aaaagg
//
LOCUS       DAVHIU010000014        27083 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVHIU010000014 DAVHIU010000000
VERSION     DAVHIU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.014"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(138..1364)
                     /locus_tag="VVN06_000517"
     CDS             complement(138..1364)
                     /locus_tag="VVN06_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU5562120.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     QYTQTPKTVCLNSATAALELILRVLEVGPGDEVVVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDLFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPAGSIADFTSFSFHAVKNFTTAEGGSATWKANPAID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYS
                     GLLQRRKDIVDRYDRGFAGSRIHPLAHKTETVESSRHLYITHVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMANYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTSSKK"
     gene            complement(1407..2099)
                     /locus_tag="VVN06_000518"
     CDS             complement(1407..2099)
                     /locus_tag="VVN06_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5562121.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVV
                     LVLTSPIFLIMSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     PAGITSPASIHYKDEDTIISQMTEKGLSVDQAYVEYVLPEKMRYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            complement(2283..4025)
                     /locus_tag="VVN06_000519"
     CDS             complement(2283..4025)
                     /locus_tag="VVN06_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562122.1"
                     /translation="MQNKKEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTMVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTTLVLIFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     VIKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDSVDIRAYSQEELRTNIGLVLQDPFLYHGTIKSNIAMYQEISDEQVRAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMS"
     gene            complement(4015..5760)
                     /locus_tag="VVN06_000520"
     CDS             complement(4015..5760)
                     /locus_tag="VVN06_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562123.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTNVHFSLEKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLAQV
                     YQDIVAMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGTHDDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(5966..6262)
                     /locus_tag="VVN06_000521"
     CDS             complement(5966..6262)
                     /locus_tag="VVN06_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU5562124.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(6415..6870)
                     /locus_tag="VVN06_000522"
                     /pseudo
     CDS             complement(6415..6870)
                     /locus_tag="VVN06_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7004..7831
                     /gene="xth"
                     /locus_tag="VVN06_000523"
     CDS             7004..7831
                     /gene="xth"
                     /locus_tag="VVN06_000523"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5562125.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            7843..8094
                     /locus_tag="VVN06_000524"
     CDS             7843..8094
                     /locus_tag="VVN06_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU5562126.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            8101..8229
                     /locus_tag="VVN06_000525"
     CDS             8101..8229
                     /locus_tag="VVN06_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU5562127.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     regulatory      8612..8707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9107..9688
                     /locus_tag="VVN06_000526"
     CDS             9107..9688
                     /locus_tag="VVN06_000526"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU5562128.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            9688..10950
                     /locus_tag="VVN06_000527"
     CDS             9688..10950
                     /locus_tag="VVN06_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU5562129.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11001..11462)
                     /locus_tag="VVN06_000528"
     CDS             complement(11001..11462)
                     /locus_tag="VVN06_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU5562130.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11459..12223)
                     /locus_tag="VVN06_000529"
     CDS             complement(11459..12223)
                     /locus_tag="VVN06_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU5562131.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12404..12808)
                     /locus_tag="VVN06_000530"
     CDS             complement(12404..12808)
                     /locus_tag="VVN06_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5562132.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(12878..14359)
                     /locus_tag="VVN06_000531"
     CDS             complement(12878..14359)
                     /locus_tag="VVN06_000531"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU5562133.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNYPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(14378..15556)
                     /locus_tag="VVN06_000532"
     CDS             complement(14378..15556)
                     /locus_tag="VVN06_000532"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU5562134.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            15721..16728
                     /gene="galR"
                     /locus_tag="VVN06_000533"
     CDS             15721..16728
                     /gene="galR"
                     /locus_tag="VVN06_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU5562135.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(16774..17811)
                     /locus_tag="VVN06_000534"
     CDS             complement(16774..17811)
                     /locus_tag="VVN06_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5562136.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(17812..18165)
                     /locus_tag="VVN06_000535"
     CDS             complement(17812..18165)
                     /locus_tag="VVN06_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5562137.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(18409..19299)
                     /locus_tag="VVN06_000536"
     CDS             complement(18409..19299)
                     /locus_tag="VVN06_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5562138.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            19432..19968
                     /locus_tag="VVN06_000537"
     CDS             19432..19968
                     /locus_tag="VVN06_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5562139.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(20135..20941)
                     /locus_tag="VVN06_000538"
     CDS             complement(20135..20941)
                     /locus_tag="VVN06_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU5562140.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(21286..22806)
                     /locus_tag="VVN06_000539"
     CDS             complement(21286..22806)
                     /locus_tag="VVN06_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU5562141.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(22799..23527)
                     /locus_tag="VVN06_000540"
     CDS             complement(22799..23527)
                     /locus_tag="VVN06_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562142.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(23542..24306)
                     /locus_tag="VVN06_000541"
     CDS             complement(23542..24306)
                     /locus_tag="VVN06_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5562143.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(24310..24753)
                     /locus_tag="VVN06_000542"
     CDS             complement(24310..24753)
                     /locus_tag="VVN06_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5562144.1"
                     /translation="MKYCHLVAHHIRLLNGRIFQKLLRQDPDSLYRSEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKNLEEQNLVTVSPCGEDKRKKYLVLTELGKSQ
                     KEVGQRVSQRLDTICYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(25435..25731)
                     /locus_tag="VVN06_000543"
     CDS             complement(25435..25731)
                     /locus_tag="VVN06_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU5562145.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(25753..26817)
                     /gene="pepA"
                     /locus_tag="VVN06_000544"
     CDS             complement(25753..26817)
                     /gene="pepA"
                     /locus_tag="VVN06_000544"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU5562146.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         7926 a         6252 c         5061 g         7844 t
ORIGIN      
        1 agaaattaat ttgactatcc tgattgattt gtcctgtttt tatttcattt tactatagga
       61 ctttcaattg aaaacaacag ttttacactc aattagactt tttagaaaca gaatcactat
      121 tacttgactg tagtttgtca tttttttgat gaagttagca ctttttcaga aactgttttg
      181 aaagtctcaa tgatatagtc cacttcttca tcacttaatt tagtgtgaag aggaagggta
      241 atttcatttt caaagaaggc ataggcctta ggatagttcg ccatgtcaaa accaagattc
      301 ttataggctg tcaagagcgg aagcggtttg tagtgtacgt tacttgcaat tcctgccttg
      361 gccaattctt ggatgataag attgcgttct tctaagcttg ctccttctac atgggtaatg
      421 tagaggtgac gtgaagattc gacagtttca gtcttgtgtg ctaaaggatg gatgcgagaa
      481 cctgcaaaac cacgatcata gcggtccaca atgtccttac gacgttgcaa caaaccagag
      541 taacggtcca attgcaccaa accaagcgaa gccatgatat cggtcatatt gcacttgtag
      601 gctggtgtga cgatatcgta ttcccatgaa cccagttgca tcttagcaag agcatcctta
      661 gtttgcccgt gaagggaaag aatttggaat tctttgtaca tctcttcgtc gtcaatcgct
      721 ggattggctt tccaagtcgc acttccacct tctgctgttg taaagttctt aacggcatgg
      781 aatgagaagg aagtaaagtc agcgatagaa ccagctggtt gtcctttata agtagatccc
      841 aaagcatggg cactatcaga aacaatcacg atacggttaa aggctttttg ccacttgctt
      901 gaagcagtaa agaggtcacg tttcttctcc actacttgga acaaacggtc atagtcgcaa
      961 acaatccctg caagttctac tggaatgatt accttggtct tttcagtgat agcttgctcc
     1021 agcaagtcat aatccatctc aaacgtatct gcttggatat ccaccatgac aggtgttgct
     1081 cctacgtgag tgatgacact acatgaagct gtataggtca tagctggaac gacgacttca
     1141 tcaccaggcc ccacttccaa gacgcgcaaa atcaactcaa gagcggcagt cgcagagttt
     1201 aggcagacag tcttgggtgt ctgtgtgtat tgagacaagc gacgctccaa ttcttttgtc
     1261 ttaggaccgg ttgtgatcca accagaacga agggtatccg ctacttcagc aatttctgct
     1321 tctgtaatat cgggtggtga aaatggaata ttgtaatttg gcattgattt gctcctttta
     1381 tctgtactca ttttctcatg actactttat tttagcactt caaacacggt ttgaaacatg
     1441 attttgatgt ctccaaggaa actaaactct cggagatagg ctaggttata gcgcatcttt
     1501 tcaggtagaa cgtattcgac ataggcttgg tcaaccgaca gacctttctc cgtcatttga
     1561 ctgatgatag tgtcctcatc cttgtagtga atgctggctg gagaggtaat ccctgctggc
     1621 aagagcaagg tcgccatcat ttcagggcta tactgctctg tgtaacgagg cacttcagga
     1681 cgcgtcccta caaaagacat ctcgccttta aggacattga ccaactgagg cagttcgtcc
     1741 aaacgcacac ggcggatgaa attaccaacc ttggtaatgc ggctatcgtt agcagaagtc
     1801 actagacttc cttttttatc cgcatccgtc accatggtac ggaacttcca aatcttgaac
     1861 cgacggttgt actgggtcac gcgctcttgc ttgtaaatca ctggcccctt gctatccaac
     1921 ttgatccaaa tgctcatgat gagaaagatg ggagaggtca gaaccagtac aaccaaggcc
     1981 aaaacccagt ccaaacaacg cttgaaaatc agcgaaccct tccttttaga gacaagctgg
     2041 tagtaagact caacctcgct tgatttcatt tccacgggca agtcttccca tttcagcatg
     2101 ctgtttctcc tttgttttta ttatactatt tgtcaacatt tttcattata ccacaaaatg
     2161 gaaaaggcgg tgaaagaaat ctgtaatttg aaggatttcc ttcttacact caatgaaaat
     2221 caaagtacag cttttaggtt gcagataaag ctgacatggc ttgaatagat tttcaaagag
     2281 tattaagaca tggctcctgc ctgcaagcta tacatcttgt gataggttcc tcccaaggcc
     2341 aaaagttcct catgggttcc gctctcgata atgcgcccct tgtccaaaac atagatacag
     2401 ttggcgtctt ggatggtaga gagacgatgg gcgatagcaa tggttgtccg tccctgtctc
     2461 atcttagcca gagaagcctg aaccagactt tctgtttcag agtcaatatt ggctgtcgct
     2521 tcatccaaaa tcaggatttt aggctggctg gcgactgttc tggcaaaggc aagaagttga
     2581 cgctgacctg ttgaaaaact ggagccacgc tcggaaacag gggaatcata accctgagga
     2641 agttcttgaa taaaggaatc tgcatccacg aaggctgctg cagcccggac ctgctcatca
     2701 ctgatttctt ggtacatggc gatattggac ttaatagttc cgtgatagag gaagggatcc
     2761 tgcaagacca gaccgatatt tgttctcagc tcttcctgac tgtaggccct aatatccaca
     2821 ctatccaaga gaactcgccc tgactgaaat tcataaaagc gcatgaggac attgataatc
     2881 gaagatttcc ctgaacctgt atgacccaca aaggcaatgg tttcaccctt attaacggag
     2941 aaggaaatgt catccagaat cggatgtttt ccatcataag agaaacatac atgttcaaaa
     3001 cggatattgc cttctttgat cacggcctgt ccattttctt gaagaggctc ataggttctc
     3061 tcgtcaatca gagcaaagac acggcctgca gaaaccatgg aagtttgaag ggttgaaaag
     3121 ttttgcgtca cctcaatcaa agggtcaaag aggcggttaa tgtactgaat aaaggcatac
     3181 atagtcccgg cagttatccc gatagaaaaa ccacggtagc caaagtaggc catcaagact
     3241 gcatagccta gaagtttcag caaactcata gcagggcgca aaaagagggc atccaaggct
     3301 acagaacggt tggcatagac caagtgctct tggttgattt catcaaattc tgcctgcagg
     3361 cgcttctcct gattaaaggc ctggattatt ctgattccct cgatgttttc agccagctta
     3421 ctattgatat ctgataagag acttctggtt ttttcgataa tcttgacgga ttttttccga
     3481 tagagattga ccaaaaggaa aatcaaggga agaaagatca agactaaagt cgtcaaacga
     3541 aaatccaaca ccaacatggt ataaagggtt gtcagaaaga tgaaaactgc tgagataaag
     3601 ctggacaaaa tccccgaaaa catatcactg atggtctcgg tatcatttgt caaacgagag
     3661 acgatagagc ctgctggcgt cttgtcaaaa taggacatgc ccaatttctc catattagca
     3721 aaggcatccc gacgaatatc cctaacaata ctgtaggaca cccgtgcaaa gagaagatta
     3781 ccgacatact gtaccatagt ttgtaggata taaaggccat agtagaccag caaaacggtc
     3841 acagcgagtt ggttgagatt actcagatac tggtcaataa agtgggaagc cacaagggga
     3901 atgacacttt tgatgaccgt cgtcgctagg agaaaactga gtgccaaaaa agtcaggagt
     3961 ccataaggct tgagataaga catcaagcgc ttcaatacag cccattgttc tttcttattc
     4021 tgcatcttct tctcccttca tttccagttg ctgagactgg taggtttggg cataccagcc
     4081 atccaaagct agcaagtcat cgtgtgtacc tcgttcgatg atttgaccat tttgcaggac
     4141 taaaatcaaa tctgcatgta caactgcact gaggcgatga gccgtgataa tggttgtctt
     4201 gtccttacgt gtttccttaa gattgtcgat aatcgcatac tctgtcttgg catccacggc
     4261 cgacaaagaa tcatccaaaa tcaagatatc agggtctaaa atcatagccc gactcatggc
     4321 cagacgctgc ttttgacctc cagaaagact gactcctttt tctccaatca gcgtatcaaa
     4381 tccctgaggc atggctacaa tatcttggta aacttgggct agcttagttg cttcctcgac
     4441 tgctgaaagg ggcaagttag gattgccaaa gcgaatattg tctaaaatcg aagtcgcaaa
     4501 gaggaactga tcctgaggaa catagcccat aagactacga agatctgtca gacgatagtc
     4561 acgaatatcg tgaccattta gataaatggt tcccttgtcc acatcgtatt cacgcaagag
     4621 gagcttgatc aaagatgttt tcccagagcc tgtttgccca accaagccca gggtttgacc
     4681 tttttccaaa ctaaagtgaa catttgtcag tgtttcctca ttttcaaagg caaagctgtc
     4741 aatggtatac tccaaacgcc cattttcaat accatccaga ggaaactcag ggtcttgtac
     4801 aggagattcc tgagacaaaa gattttcaat ccgctggtaa gaaaccttcc ctcgctgagt
     4861 agtattaaag aggaagccga tggccataag aggccagacc agcatatcca aatagctgat
     4921 aaaggtgact agattcccaa ctgtaatctg cccttcctga accatcaagg agccaaccaa
     4981 aagcgttaaa acataggacg aaccaacaaa cacgagaacc atagggtcaa agagactatc
     5041 atatttcatg gtttgcaggt tcttttggaa ggttaattca ttgactgcct gaaaagactt
     5101 caactcgtct gcctgataac cgaaagactt ggtcactttg atacctgata cggactcctg
     5161 taccttgtta ttgagttcag aaaaagcagc ttgggattcg ccaaaggcct tatgagtctt
     5221 tctccctagg cgactagttg cataggccat gaaaggtagg gggagaatgg caacaagagt
     5281 catctgccat gagatgctaa agagcatggt caacaaagtc accagagccg tgatagaggc
     5341 atccaccgca gacatgacac cgccacctgc taaacgagtc aaggcattga tatcattggt
     5401 tgcgtgtgcc atcagatcac ccgtccgata ggtttgataa aaggctgatg acatttttgt
     5461 gaaatgctta aacaagcgag accgcatgat ctgtcccaag caataagagg tcccaaggat
     5521 atacatacgc cacacatagc gcaaatagta cataccaaag gctgcaagta gcaagtaaaa
     5581 taggctaaga aggaggtcct gctgggttaa ttgccccgat gtgatggcat caatgacccg
     5641 ccccataacc ataggaggaa tgagattgag gacggaaacc aagaccaagg ccacaatccc
     5701 gactagataa cggcgttttt ctaacttgaa aaaccaccag agtttttgaa taatggacat
     5761 aaaatccctt tctgatcgca aatggaaacc tgaggccaat actcaatgaa aatcaaagag
     5821 caaactagga aactagccgc aggctgctca aagcactgct ttgaggttgt agatagaact
     5881 gacgaagtca gtaacctaca tacggcaagg cgacgttgac gcggtttgaa gagattttcg
     5941 aagagtacaa gaccccaggt ttttcttatt cataagttac gactgtaaca gcacccttgt
     6001 catattcagc aataaagata ttggctacat tgtcatgccc ttgtttgctg aggttatcaa
     6061 gcaaccactc ttcgctacga ccaatcgatt ccaagacatc tacttgaatc acaccgtcag
     6121 tcacaactgg atacttagga ttttcatctc ccatttgcac aacgatgagt tgcccatttt
     6181 gctcttgcac agctcgtttg acttgcttca tctggaaaat cccttggcta cgaagtttga
     6241 gggctacatc cgatgcagac aaaccaactg aacgacaggc ttatgggtca atattcccat
     6301 ttttgataag gagagttggc ttaccatcaa tcaaacgttt cacaaagtga acattgttgt
     6361 tgagctattt gagagtcaag accagtttga ttaaaatttc gatataatta agtgtcattt
     6421 gttcacatcc accagttcaa ttgcgtctgt cttatacaat tcgactttct ctaacaggta
     6481 cttgtctggc tcacttccgt ttagggcacg ataatagcga tctcccacct tgataagtgc
     6541 gccatctgtg tttgtggaag tattaacata gacttctgac ttgtctactc ccaaatcttt
     6601 ggaaacaacc tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa cttgattgtt
     6661 ttgataagtc gtgatgctga ccaaaagcag agctaaataa ggccagagct gaaatcatga
     6721 ccaactcacg aaattcggtt ccctttttat catggtatgc cttaaaagca aaaaaactgt
     6781 cacagctaat agaacaatcc caaagccaac catgatacca ttttgctgac cgatttggct
     6841 gagtacatag tcataagagt agaatttcat gtattttcac tccctttcat aaaatcattc
     6901 ttaattataa acgaaagaga gtaatttttc aaactatacg ttggggaaat agatcttttt
     6961 ttggtataat aaaatctata atctgaatga aaaaggtaac tttatgaaac ttatctcatg
     7021 gaatattgat tccctaaacg ctgccctaac tagtgactca gctcgtgcca aattgtccca
     7081 agaagtccta caaaccttgg tcgctgaaaa tgctgatatc attgctatcc aagaaaccaa
     7141 gctttctgcc aaaggaccta caaagaaaca cgtggaaatt ttagaagaac tcttcccagg
     7201 ctacgaaaac acgtggcgct cttcccaaga gcctgcccgt aaaggctatg ctggaaccat
     7261 gttcctttat aagaaagaac ttacacccac tgtcactttc ccagaaatcg gtgccccttc
     7321 taccatggac ttggaaggcc gcatcatcac tctagaattt gatgcatttt tcgtgaccca
     7381 agtttacact ccaaatgctg gcgatggtct taaacgcttg gaagaacgcc aagtctggga
     7441 tgtcaaatat gcggagtatt tggctgaact agacaaagaa aaaccagtcc ttgcgaccgg
     7501 tgactacaac gtagcccaca atgaaatcga ccttgcaaat cctgctagca accgccgttc
     7561 acctggattt actgacgagg aacgtgctgg atttaccaac cttttggcaa ctggatttac
     7621 agacaccttc cgccatgttc atggcgatgt tcctgaacgc tacacttggt gggcacaacg
     7681 cagcaaaact tctaaaatca acaatacagg ctggagaatc gactactggc tcacaagtaa
     7741 ccgtatcgct gacaaggtca ctaaatctga tatgattgac tcaggtgctc gccaagacca
     7801 tacaccgatt gtcttggaaa ttgacttgta agaattttct atatggacta caatgcggtc
     7861 attcccgagt tgcttgtatc gaatatcgaa cagtcccggt ccttctactg tggtttactg
     7921 ggttttagga ttgaatacca acgtccagaa gaaaactttc tctttctttc tcttgaagag
     7981 tgtcaactaa tgttagagga gggaacaaag gatcaactag ctgaactgac ctatccattt
     8041 ggtcgtggag tcaatctctc ctttggcata aaggatgttt caaaactcta ttaaagagtg
     8101 atgaaggcaa actatcctat ttatcgcccc ttgaccaaaa gaaagtttcg tgtcagtgat
     8161 ccctatatct atcctcataa atttgcagtt ttggatccaa atggctattt tttaagattt
     8221 agcgagtaga ataataaaaa ttgggaaaag tctttaaagt tagaaaaagg catattgtca
     8281 ggtgtttaaa aacttgatag tatgcctttt atgatggaaa aatcttctaa tactcttcga
     8341 aaatctcttc aagccacgtc aacgtcgcct taccgtaggt atatgatact gactctgtca
     8401 attctatcca caacctcaaa acagtgtttt gaggttgtgg atagcttcct agtttgctct
     8461 ttgattttca tttagtataa atattagctt acaatggagt ttttgttcac cctctcttct
     8521 gtttgttcgt tttttaaacc aaaacactaa caaaatagtt gatttataaa gttatttagt
     8581 ataaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta cttgtttatg
     8641 tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa gtaagtcagc
     8701 agtgagatat ggccgttttt gccatgccta tttgggggac ttacttagag attaagtagg
     8761 tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt gaaattatcc
     8821 tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc ctagctcttt
     8881 catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa aatcccgtca
     8941 tagacaataa caagatcatt gtctatgata ccactacaac gggaaatatt tgataagact
     9001 attacgagcg aagcgagtct tatcaaatat atagtattag caaaagggat cactatagaa
     9061 acatttcaac aacaagtaaa ttagctttta gactttagga ggtcttatga aattattaga
     9121 agatcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg tagattcctt
     9181 tttaacccac caagttgact ttagcttgat gcgagagatt ggtaaggttt ttgcggaaaa
     9241 atttgctgct gccggcatta ccaaggtcgt aaccattgaa gcgtcgggta ttgccccagc
     9301 cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag ctaagaacat
     9361 caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc aggtgaccag
     9421 caccgtttct atcgctggaa aattcctctc accagaggac aaggttttga ttatcgacga
     9481 tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac aggccggtgc
     9541 cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggtc gtgatttgct
     9601 tgaaaaagca ggctaccctg tcctatcact tgctcgcttg gatcgttttg aaaatggtca
     9661 ggtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc gcaagcagcc
     9721 gttcttggct tgcagcactt actagccatg tactcaggat ctatcctggt tcccatcatg
     9781 attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc tacagatatc
     9841 ttcatgtgtg gggtggcaac cttcctccaa ctccaactca acaaatactt tgggattgga
     9901 ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat gattgggcaa
     9961 agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta cgtggttctt
    10021 gtttcaggca tcttctcaaa agtagccaat ctcttcccat ctatcgtaac aggatctgtt
    10081 attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg aaataacgtt
    10141 ccagagccaa ctggtcaaag tctcttgctt gcagctatta ctgttctgat tatcctcttg
    10201 atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg tctggttgtt
    10261 ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc ggtagctcca
    10321 cttgtccatg tcccaattcc actctacttt gggatgccaa cctttgaaat ctcatctatt
    10381 gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt ttatctagcc
    10441 ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg ttaccgcgca
    10501 gaaggtttgg ccgtacttct cggaggaatc tttaacacct tcccttacac cggattttca
    10561 caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat ctactacgca
    10621 gctggtttcc tggttctcct tggactgctt cctaagtttg gtgcccttgc ccaaatcatt
    10681 ccaagctccg tcctcggcgg tgccatgctg gtgatgtttg gttttgtatc tattcaaggg
    10741 atgcaaatcc tagcccgagt tgactttgct aacaatgaac acaacttcct tatcgcagct
    10801 gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag catgccgaca
    10861 gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctactcgc cattgtcctc
    10921 aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc ttttttgtgt
    10981 gacaaaataa aaacatctac ctattcatat atgctagtgt catagtcctg aaaatcatct
    11041 gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc ggcttcatct
    11101 ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc tactgtatac
    11161 tttttcagtt ttagattgct ttccaataat tcacctatta acaataatct atgataggag
    11221 ctatgtagcc actctggcaa tttatctcct acttcccacc catatttttc cttcatcaaa
    11281 gctagttgtt tttcgtcacc aaatttcata ttttcagata accatctcac acaatcggga
    11341 tctgataagg tacagacgat taaacttagg ttatcgtaac ggctttcagt ttcataatca
    11401 ggccagatga cttttaagaa atcatacacc tcttctgatg caacaagttg tttcgttttc
    11461 ataatttccg atactttcct ctacctttaa attctattat ttctttgtct cttaatattt
    11521 gaagctgttg cctaatcttt tctttgatat gattgttctt aacaaagata ttttttaggt
    11581 cactttcaaa acgatacata tcttcaaggg taaattctga accctctatc ttatctatac
    11641 aatttagaat ttctattgtc caacctcttg atgacagaga gctcttcctt aaaaataaac
    11701 cttgcttaaa ttcttttgta actttttctg gatctctaac ttgtccatct tgcacaagaa
    11761 atatccttcc tttagaaggt acttgtgata aatcaatgtt acaaccaatc caacctgctc
    11821 gtctagcagt tggtgcaagt ggttttcttt gaataatcga tttcggtgta acaaattgct
    11881 tcggaaggac aagaaagtta tttacctcaa aattttttgt gtaagttaaa aaaaagaaat
    11941 taggattatt atctgcctgc acacgcttca tcatcgttgc ataagcacca tcattgattg
    12001 ttgatgaaaa atttcctttt ttgctcttta gttcaaactc ctcactacaa tgattacagt
    12061 aaaaatctgc tacaggccga ttattttcaa aatgatttaa gggattgttc ccacaattag
    12121 ggcaataaga ctgacgataa acccaatcct ccgttaagat acgtgccttt tggctattac
    12181 ttttataggt ttctactaat tctaaattaa agtgtaattc catttaaaaa ttcctcacta
    12241 atatgagaac tcactcgata taagcaatta aaatctacta taactagtat accacatata
    12301 tgtacctatt acatttgtat ttcatcactc tcttttatat ttccttctaa catatctcat
    12361 aaattaaaaa tgagtgagat aaatttatcc cactcacctt tctttatata cttacccgtc
    12421 tttcctcaga cctttggcct gtgacaaaca tggtaaaggt tgctttacag acatttctgc
    12481 cctcttgatt ggtgatatcc acatccatca cacaggttgt acggccttga tggacacatt
    12541 ctcctttaat ggtcaataca tcgtcgagtt ttcctgcctt aaggtagttg atagaggatt
    12601 ggagtgtcac cccatcaagt cccagcgaga taaccaccaa accactgatt tggtcacata
    12661 gcgtgaatag atagccacca tgggcattgc catagtagtt gagcgacgag ttcactactt
    12721 tggtcgtcac cacaacgtga ccatctctca ttttttcaat ttcgtaattt tcaaaggcag
    12781 atatagcgtc aaaatgaaag tctttcatcg tttcctcctg atatttcaaa cgacactatg
    12841 atactacttt ttataggact gtgcaaggag aaagctccta gtctagtaaa attccgacat
    12901 gttccacaaa gcgcataaag gctgtctgcc cttgttctgt ctgcttgtag actcctgcat
    12961 cctcaagtac acgcgcaaag atagcaccca cagagtcctt gacgattgca agggcttttt
    13021 ctttatccgt taggtctgga tgttgggatt tgagttggtc tgcccactcc tgatgataat
    13081 cggcaactgt aacagcttct cctacaagat agctcgcgac ttgctccact tcttctttca
    13141 gacgtggtgg caagattgcc aagcccatga cctcaatcaa gccgatattt tccttcttga
    13201 tatgttggac atccttgtgg ggatgataga tgccatcagg atgctctgct gaagtctgat
    13261 tgtctcgcaa gaccaagtcc aactcaaact gtccatcacg cttacgcgct attggtgtga
    13321 tggtgtgatg cggtgtcccg tctgtctctg ctaaaatctg cacactagaa tctgaatact
    13381 gacgccattc ctggaaaatc ttatcagcta agttaatcaa atcctctttg gaatccgaag
    13441 tcaagcgtag gacagacatt ggccacttga taattccagc cttgacctgc tcaaaaccag
    13501 caaatcggaa ggtcttttgc aagggagcca attccatagg aaatacgtga cggcctccct
    13561 gataatgatc gtgagttaga atagagcccc ccacaatcgg caggtcggca ttagatccag
    13621 caaaatatcc tggaaactgc tctacgatag ccaacagacg ttcaaaactc tgacgactaa
    13681 tggccatggg acgatgctgg ccatctaaaa agatacaatg ctcattaaag taagcatagg
    13741 gcgaatactg gaacccccat tcctgaccaa ccatttcaaa acggataatc cggtggttgc
    13801 tgcgagctgg gtagttgacc cgaccatggt aaccctcatt ctctaaacaa agttgacact
    13861 gaggataatt actagcttgc accaacttgg ctaccgcaat ctctttcgga tccttttcag
    13921 gcttagagag attgatggta atttcaagtt ctccataatc agatggaaca cgataagcga
    13981 tatttttagc aatggccttg agtttgatgt aatcattttt ctgactgagt tgataaaaat
    14041 cctctatcgc ttgctcagga gagtggacat aggcttccca gaagtcacga ttgacctgac
    14101 tcggataagg agtcaccaag tccatcagtt cagtaccaag gatttcacgc gcagtctgac
    14161 tatcctcaat catctctaat cgaacggctt cctcaactag ctggtccttg aggtcaatca
    14221 atttatccag attggtctca acttccaaaa caccttctcc cactcgtgcc aaaacacggt
    14281 tggtcaggta gattcgatcc atttcctcaa atgaactttc agaaatgaca tgtgttacaa
    14341 atttatctac taagatcact atagagcctc cttttgacta gtcaaggacg cgagtgccac
    14401 ctgcaacttc agcgatatag aagcttggag cgtatccaac tacttcctcg tagtgtttgc
    14461 ctacagcttc cttaaaggcc tcaacagtat ctttttgaac caaggcaatg gcacagccac
    14521 caaaaccagc ccctgtcata cgagcaccga gaactccttc ttgtgcccaa gctgtgtgaa
    14581 caagagtatc caattccaaa ccagttactt cataatcatg ctccagagaa acgtgtgacg
    14641 cattcatcaa acgaccaaat gtttccaaat cacctgcttg aagggctgct tgagctttaa
    14701 gggtacgttg gttttcaagc acagcatggc gagcacgttt caaacgattt tcatctttaa
    14761 tcaaatagct atattggtca acggcccact cgtccaattc acccagggtc tgaatatcca
    14821 aggcaacttg caattcttcc actgcttttt cacactcagc acgacgttca ttgtatttag
    14881 agtccgccaa ttcacggcgt ttgttggtgt tcatgataac aacgacattg tccttcaaat
    14941 caagtggcac caagtcgtat tctaaagtat tggtatctaa gtaaatagca cgttggtcag
    15001 cccccatacc aatagcaaac tggtccatga taccagagtt gactccgata aagttgtttt
    15061 ctgtttgttt tccgatttta accaaatcca aacggtctag ttttaaatca aagagatgct
    15121 ctgccacgac tcctgtcaag agctccaagg atgctgaaga agacaagcca gcaccatttg
    15181 ggatattccc ataaacataa aaatcaaaac ctttgtcaat cacgtgccca gcttcttgca
    15241 agaaatgaag aacccctttt ggataattgg tccagttgtg ctctttttca aacttgaggt
    15301 cagcgagagg cacttcgata atgcccttgt cctcaaagtt agctgagtag aaacgcaaga
    15361 cttggtcgtc acgcttacga gctgcaccgt aagttcccaa ggaaatagca gcaggaaaaa
    15421 cgtgcccacc gttgtagtct gtgtgttcac caatcaaatt aatacggcct ggtgaaaaga
    15481 aggtttggtc tgcttcttga ccaaaaacag caagaaagtc tttgcgaagg acttcagtag
    15541 taagatgttg tgccatatga attctccttt gactgtccgt taagccatct taacgtgtaa
    15601 tcgttttctt tctattgtat ccaaggaatt tcccaaggtc aatccttttt actaaaattt
    15661 tactaaaata tcagtaaaaa gaagaaaaat atgatatact aacctaatca aggaggattt
    15721 atggctacct taaaagacat tgcacagcta gcctctgtct ctatcgcgac cgtatcccgt
    15781 gtcctcaacc gcgaccagag cctatctgtt acagaagaaa ccagacaccg tattttaacc
    15841 gttgctgaag agctgggcta caccaagcac ctcaagacag gcgagtccca taaacccaag
    15901 caaaagattg ccattattca atgggtcagc gaacaagggg agctagacga cctctactac
    15961 taccagattc gcctaggaat agaaaaaaga gcccaagagt tggactacga tatcttgcgc
    16021 tattttaatg accacccttt taccctaagc gaggaagtga ttgggattct ctgcatcgga
    16081 aaatttagcc gagctcagat ttctgccttt gaagaatacc aaaagcctct tgtatttcta
    16141 gacagcgata cactttccct gggacatacc tgtattatca cggattttta cactgctatg
    16201 aaacaggttg tcgattattt cctcagtcaa gggatggacc gcatcgggat tctaacaggg
    16261 cttgaggaaa caacagacca agaagaaatc attcaggaca agcgtctaga aaacttcaaa
    16321 aactacagtc aagcgagggg aatctatcat gatgaactgg tctttcaagg aagatttact
    16381 gcccagtctg gctatgactt aatgaaggag gccattcaga gcttgggaga ccaacttccg
    16441 ccagcatttt tcgcagccag cgatagttta gctatcggtg ccctccgtgc cctccaagaa
    16501 gctggaatca gcctgccaga tcgcgtcagc ctcatttcat tcaacgacac cagtctgacc
    16561 aaacaggtct atcctcccct ctctagtatt acagtttata ctgaagaaat ggggcgagca
    16621 ggtatggata ttcttaacaa ggaagtcctc cacggtcgca aaattcctag cctgaccatg
    16681 ctgggaacca gactgacatt aagagaaagt accctaaatc aagaatagga taacataaaa
    16741 aacgaataga gttctaaaac tcctattcgt tttttattcg attacaatca tagacttaat
    16801 tgtcttacgt tcatccatat ctttataggc ttggtcgata tcttccagtt tataacttga
    16861 agtaaagacg cgacctggat tgatatcacc atcaaggacg gcttttagta aaaattgctt
    16921 atcgtatgtt gtagcagaag ctgccccacc tgctacagag atattttgca taaatgtcga
    16981 accaagagca cgattattat agtgtgggac tcctacaaaa cccatacgcc ctccattatg
    17041 aagaacacct agcgcctgtt ctatagcagc ctccgtacca acacattcaa gtgctgcgtc
    17101 tgctcctccg ccgaggattt cacgcacctt ggtaattcct tcttgaccac gttctgcaac
    17161 aacagctgtc gcacctgact ccatagccat cttttgacgg tcttcatgac ggctcataag
    17221 gataatttgt gatgctccac gcatcttagc cgcgatgaca gcacattgac caacagcccc
    17281 atcaccgata acaacaacct tgtccccttt ttgaacattt gcaacacgcg ctgcatgata
    17341 gcctgtcggc atgacatctg caagagtcaa aagggacttg agcatccctt ctgtataatc
    17401 agaaggttga ccagggattt taaccagcgc ccagtttgca tagtggaagc gaatatattc
    17461 tgcctgaaaa tcacccccca aattattacc aatatgattg tcgcaagaac cgtcaaatcc
    17521 agcaagacag gcatcacact caccacatcc atgtgtaaaa gggacaatca caaaatcacc
    17581 tggtttcacc gtcgtaatgg cttccccagc ttcttcaaca atcccaatcg cttcgtgtcc
    17641 actattttta tgtccagctt tcgtttctgg attacggtac ctccataaat ctgaaccaca
    17701 aacgcacgca cgaaccacac gaataatcac atcatccgct tctattattt gcggacgttc
    17761 aatgctagca agtccaacct gacctgcctt tgtatatact gctgatttca tttaaaattt
    17821 tccttcctta taaagtttaa ttttgagatt taaacgattt aaagctgtct gtagctgaga
    17881 caagcgctcc tctaattttt gcttttcctc ttctaaaata ccaagcctct cctctctcgt
    17941 ttcatctccc ttttggtaga gcgacatata gtcaactaaa ctatctacag agacacccgc
    18001 cgaacgaaaa cacttaataa attccagcgc ttcgatatcc tgatcttgaa aatcacgaat
    18061 cccagtagca gtacgagtaa tcggtggcac aagaccaacc cgttcataat accgaatcgt
    18121 atccgctgaa attcccaaca aatcactggc agatttaata ttcacacgct aacctcctct
    18181 catttcattc actgaattca cgctattcta actcacttat cttattatat aactttgagt
    18241 tgactccaag tcaagcattt tgtatctaat cgaaaaattt ttatcattaa caatcatata
    18301 tctggagttt atacaatgct cttctcttac caatttttga aatatttaaa taataaagaa
    18361 attactctat tccaaataag atagtaccca agtatttttc acttttttct aatgttgatg
    18421 ctcataactc cgttccaagt cacacacctt tcgcttatgg gtttggtgag tttctaggtc
    18481 agcatcaatt tcaatggtaa tattttgaaa accacaatct tttaggaaaa ttcgaataga
    18541 ctctttacaa gtttccatat gttccatttc ttttagacaa acatggacaa tggcattttt
    18601 ttccaaagca tccatagtcc agagattaag ctgattaagg ctggccacat tgtccaatcg
    18661 ctccaggcca ctctttactt gcttgatatc aagaccttct ggcacagcat ccaagaaaat
    18721 cttgagtgta gaccaaaaac gtggaagggc ttttgaaaga ataaagaaag aaatgacaag
    18781 ggacaaaaga ggatctagga tataccagtc cgtaaatcga agaacaatcg ccatcaggat
    18841 aacagctacc caccctagcg tatcttccag aaaatgcaga ctcagaatag actcattctt
    18901 tgtctttccc ttaccaacca ccagactcgc taacagattg atagtaatcg caataattcc
    18961 taaccagaga atcccctcat cattgactgg ttgcggatgc aaaatcttcg tgacattttc
    19021 caaaatgact agaacagagc ccgttacgag aatcacagct gttaccaagg ctcctagcag
    19081 gctaaaccgc ttatagccca aggtgtactg attgtcttct tcacgattgg agattgtttc
    19141 tagaaaagct gatattccaa ttgcaatcgc atctcccaag tcatgcacag agtcagcaag
    19201 aacagcgcta gaaccaaata ctccacctgc aataaactca acaatggcat aagtcaaatt
    19261 taagaaaaaa gccacccaaa cagcatattt tgccttcata tttctcattc ctttgttata
    19321 atagatttat gaacaccttg ttcattatca ttatccacta aaactcaaag aaaggataga
    19381 agcaaaacgt cagctttatt cagttctgaa caatttgcct taagtgtcca tatgactaac
    19441 attgaccgcc gtatcagcaa aaccaaaaaa gccatctatc aagcttttat acaacttttg
    19501 aatgctaagg gctacgaggc cactactgtt caggatatca ttgatctcgc agatgtggga
    19561 cgatccacct tttactgtca ctatgagagt aaggagcttc ttctggacca gctctgccgc
    19621 tacctcttcc atcatctttt tgaaagagag caagccattt caaccgagga ctacctcgcc
    19681 cacctctttc tccattttca taaaaaccaa gaccatatca ccagtctgct attttccaaa
    19741 aatgactact tcctccgtca actccataaa gagctagaac accatgtcta ttccgtgcta
    19801 gctgataatt taaaagaagc ccaccctaat ctgcctactt cttacctcca acacttggtc
    19861 atgtccaact ttatcgagac attgacctgg tggctcaaaa aaggtcaaga tttcacagac
    19921 caggaagttg tccaatttta tctagacctt ctcattccta aaaattgaat atagagtaaa
    19981 gcttcagttg tcttatttct aggttactga gttttttatc ttttcaacaa caaaagagga
    20041 cccgccgatc ctctttttca tactataaat ccttgattat caactatatc tgttttaatc
    20101 gaaatctcaa aacagcactt tcaaacatct tttcctagtt aagtaaatca gtattctgct
    20161 tagctgcctt gctccattga taccaaccaa ctagactgtt aatgagataa attagatatt
    20221 tcccttgaat ttgcaggctt tctccccacc agagatagat tgaaaagaca ttggtagccg
    20281 cccagaatat ccactgttca cggtaaacag ctgtcatgag gatttgccct accccattgg
    20341 ttgcatctgt gattgaatca cgatagggac gattggcacc aatagactga taaatgaagc
    20401 caaaggccaa ccaccaaagc acactaatgg aaagatactt tgtccagccc ttgccgtcca
    20461 gtttacgcgc gacaaactcc tgcttttcct tcttaaactg tgcctgataa atccaaacta
    20521 gaagtccaat tggctgcatg actgtgaagt aaagtgtcgt cagcacctca ccataaaagc
    20581 ctttctgtag ggccaaaata aggtaaataa cagagttaat caagccaaaa agataattac
    20641 ttgctcgacc ttccgataca aagattacac agataatccc tgtcaagcta caaatcatcc
    20701 caatccagtc aacaatacga tgttcgtaaa ccaactccag ccagagagga aaacttccta
    20761 aaaccagcaa ataaatccac tgggcaaaac tacgatgggc aaagaggtca tcccagatag
    20821 ccttcatagt tcctgaaaat cctaaatcag ccatagccgc aaccatacga cggtaaccac
    20881 ctgacatttc acctagggtt gttttgatat tttcaatttt cttttgcaaa taagtatgca
    20941 tcatttctcc ttttgttttt aaagagccgt gtctggatag actttcggac gcaacgctct
    21001 attagataat gaactgccta tacacaagat ttctaacctt agtcgacatg agctgaaacc
    21061 tcttatttgt taagtagttc acaaaatatt atacacctat tttatgaata gtcaactgtc
    21121 tttacagtaa aattttagaa aatcatgaaa attttctctt tctttccatt ttaagtgaca
    21181 ttcagtcatt ctcacatcaa aaaagcccag acgaaattgt ctgagcattc ttttatctag
    21241 tcgtttaagg aagttgagtt cagtatgttt aaagtctctg tcccatcatt tcttcaacaa
    21301 accttgttct tggagaaact ccttggctac ttgctttgct gacttgcctt caacaccgac
    21361 ttggtagttg agctggctca tctggctttc tgtaatctta ccagccaatg tattaagaac
    21421 tctttccaac tctgggtgtt tcttgagaag agcttctttc atgagtggag ccccttgata
    21481 aggtgggaag agttgcttgt catcttccaa gacctgtaaa tcataacgct ccaattccgc
    21541 atcagtcgaa taggcatccg tgatttgaat atcccctgac tgaattgcct gataacgaag
    21601 agctggctgc atagtcgcca cattgagatt gagaccatac attgattgca agcccttatt
    21661 tccatcttca cggtcgttaa actcaagcgt aaagcctgcc ttcagctgcc cttccacttt
    21721 tttcaaatcc gaaatggtct tcaagccata ttcttgagca atctttttcg gaacagctac
    21781 agcataggta ttttgataag acataggttt gagataggct agatgatcct gcttggcaat
    21841 gccatcacgc gccacttgat aaacctgctc tggctcatga ctcaccttgg gtgatggttg
    21901 aagcaaactt tcagtcaccg taccggtaaa ttcaggataa atgtcaatat cacctttttt
    21961 cagagcttca taaaggaaac ttgtcgtccc aaaattcggt ttaacagtcg cagtcatgct
    22021 ggtattttct tcaatcagca atttatacat attagccaaa atttctggtt ctgggcctat
    22081 tttcccagca ataatcaagt tttccttctc tttttgagcc aaaagggctg gactataaga
    22141 cagacccagt aataaagcca ccaaggcaaa accagagaaa atcgtccgca atttcgcttt
    22201 ttccattact tttagtagga agttaaaggc aatagctagc actgcagaag aaagggcccc
    22261 aatcaaaatc aaactggcat tattacggtc aattcccaac aggataaagg aacccagtcc
    22321 ccctgcccca atcaaggtcg ccaaggtagc cgtaccgata atcaagaccg ctgccgtccg
    22381 aattccagac atgataacag gcatggcaag tggaatttca aacttcttga gacgctccca
    22441 tctggtcatc ccaaaggcaa tcccagcctc ttgaagactc ggatcaattc ccttcagccc
    22501 agtaatagta ttttgtaaaa tcggaaaaat cgcataaatc actagagctg tcaaagccgg
    22561 caaggtccca attcccatca aagggataaa gagccccaac aaggccagag acgggatggt
    22621 ctggaaaata cctgcaatct gcaagaccca gtcggccagc ttctcatgat agcgaagaaa
    22681 aacagccaag ggaatcgcaa gcaaaatagc tagtaacaag gtcaaaagtg acaactgcaa
    22741 atgttgagat agagctgtca accaatcact aaaacgatcc tgaaaagttg caattaaatt
    22801 agtcatgaac actacctcca aacaagtctg ctacaaagtc tgttgcaggc acttttaaaa
    22861 ttgtctcggg attcgctacc tggcgaattt ctccatcctg caagacagca atacggtccg
    22921 ccaacttcaa ggcttcatcc gtatcatggg ttacaaaaat cgttgtcatc ccaaactctt
    22981 tatgcaattc ttttgtcaga acctgcaact gttttctcga aatagcatcc aaggccgaaa
    23041 agggttcatc catgaggaaa atcttgggct gaccaatcat agctcggaca ataccgaccc
    23101 gttgctgttc tccaccagat aattcactag gtaagcgatg cccatactcg gctactggta
    23161 aaccaacctt agccaaaagc tcttctgttt tcttcgtaat ttcttccttg ctccacccct
    23221 tcatttcagg aatgagagca atattttccg caactgttag atttggaaaa agagcaatag
    23281 cctgtaaaac ataaccagta gaaagacgaa gttcacgctc atcatagtct ttgatgcgct
    23341 tcccatccat ataaatattt ccatcagttg gttccaaaag acggttaatc atcttgagca
    23401 tggtcgtctt acctgaccca gaaggcccta ctaaaaccat aaattcccca tcctcaatct
    23461 gtaagttgac atctctcaag acatcctttt ctgtgtagcg cagtgctaca tttttgtatt
    23521 caatcattct ttgtcctcaa tttaaaactt ccctcgatta gtcaagtctt ctaccttagg
    23581 catgacttcc ttattatccc aatgctccac aattttcccg ttctctaaac ggaagatatc
    23641 gtactgggca taagcaacgc catcaatctg agtctgacca tagctaacca catagtttcc
    23701 ttgtcctaag agttggaaaa caaagtcaaa agtgacacta tattcagcca catagttttt
    23761 ataagcagca cttccttgtc caatctcatg attatgctga atcaaatcgt ctgccacata
    23821 atcactccac tgctctagct ccccattttg gaaaatttct gtcaagaaac ggcgaaccag
    23881 ttttttattt tctgctttct tgtccaaatc cttgatttca aaatctccaa aaatttgatc
    23941 tagttgatca ttttcaggtg ttcgatagta gtcaatgaca tcccaatgct caacaatacg
    24001 accattctca tccgcacgga aagtatccgt cgtcacccat tgagcttctc caccattcag
    24061 atattgatga acatgaacaa agaccagatt gccatcctca atggtgcgga caatcttaat
    24121 ctgacgctct ggatgacgct caaagaaatc tgcaaagaag gctgcaaatc cttctttccc
    24181 gtcaggaaca cctgtcgaat gttggatata ggtatcccct acagactggg cttgagcctc
    24241 agcaactcgt ccgtcttgaa tggcatggat gtataggttg tgagcatttt tcacttgttg
    24301 tgacatattc taaacctcat ttcccttctc tttcaaattc gccaaaattc tttcttgaaa
    24361 accttcaaat tggtgaattt cttcctctga aaatcctttg taacagatag tatccaatct
    24421 ctgactgaca cgttgcccaa cttctttctg ggacttgccc aactctgtta aaactaaata
    24481 cttcttacgc ttatcttctc cacacggact gacagttaca agattttgtt cctctagatt
    24541 ttttatcata gtcgtcagcg tattattagc aagcccagtc gcaagcgcaa tgtctgtcgc
    24601 cgtagcacag cctgtctcac tattccataa aaccgctaaa atctttccct gctcactccg
    24661 ataaagagag tcagggtctt gtcttagtaa cttttgaaaa atccgcccat tcaacaaacg
    24721 aatatgatgg gctaccaaat gacaatattt catgaaacct ccaatttatt tctatatcga
    24781 aatagataaa gtgattataa cactcatcat tccatatgtc aactatttcg atttagaaat
    24841 aatttttgat aattatttac atcaccatac tccggatcaa ctaactttta aggagagttt
    24901 ctaaactcct tcgtcctcca gtctacaaaa gcctttcatc cgtactatcc tatattttat
    24961 gaggggacac atttttccta tcagaccatt tattttaaag atagaagtaa atcataattg
    25021 cttccatctg ttcttttata gtatattgaa gttgaactag gacactgtat cttctaaaac
    25081 attgataaaa agcgacttga atttcccaat caatttgttc gtatttatag catttcgaaa
    25141 ctggaatagg acaccatgac tgctaaaaga tttctataaa ttcatttgat ttccctaatc
    25201 aatttgttca tatcttattt cattccgcta taatttcacc ttaccctatc tttttcgtag
    25261 cacccttcaa acagcctatc ccctaccgtt tgacgattcc tcacttcact ccacttccat
    25321 tacagaagtt tcttcactac tatggtctcg gctgacttct catgattcct tgttactact
    25381 atttgaacgc tcacgagata ggtcttataa aaaatgcttt gatccacaat ggaatcaaag
    25441 cattttaaaa agttcctcat acataagcgc agaagtcgca gttcctctgt acttggcttc
    25501 ttctcttttg ataaagcgag ccaagttgag caactcaggt gctggatgtt tgggatttag
    25561 gagcaattca cgattgacca ggcctgagag acgaactgcc tgcaattgct catttgtagt
    25621 aggcagtttt ttagtagtct ctaggagagc agcaactaaa tcttcactca aatcatgtcg
    25681 agcatgattg taaagatctt ttataaggct ttctaggttt ggttctacca tccctaccac
    25741 ctcccttatg gtttaataat gtttaatcaa atcaaccgtt gaacgatcca atttcttcac
    25801 caaggcttgt aagaaagctt gcgcttctag gaagtcatcc attgcataga gggtttggtg
    25861 agaatggata taacgagcgc agacaccgat agttgttgat gggacaccac catttttcag
    25921 atgagctgca cctgcatctg ttccgccttt accacagtag tattggtact tgataccagc
    25981 ttcttcagcc gttgtcaaaa ggaaatcctt catccctgga agaagcaagt gacctggatc
    26041 atagaaacga atcaaggttc catctccaat cttgccttga ccaccgtaga catcacctgc
    26101 tggtgagcaa tcaactgcga ggaagacttc tgggtcaaac ttggttgtag aggtatgagc
    26161 gccacgcaga ccaacttctt cttggacgtt agaacccaga tagagttcat tgccgagttt
    26221 ttgacctgat aaagcttcag ctagctcgct taccatgagg acaccgtagc ggttatccca
    26281 agcttttgag atgatatttt tttcattggc tgtcaaaatt gcagaactat ctggtacgat
    26341 ggtatcacca ggacggatgc caaaactttc tgcctcagcc ttgtccgcaa aaccaccatc
    26401 aaaaacgata tcggcaatgg ctggcatggt tggtcccccc tttccacgag tcaaatgcgg
    26461 aggaacagaa cctgaaatca caggaatttc atgaccatca cgagtcaaga gtttgaaacg
    26521 ttggctgcta accaccatgg ggttccagcc accgatttct acgacacgga aggtaccatc
    26581 tggcttgatt tcgctgacca taaaaccaac ttcgtccata tgagaagcga ccaagacgcg
    26641 cggtgcatcc acagcttctg aatgtttgat accaaaaata ccacccaagc catctgtcac
    26701 cacttcatcc acatgcggtg tcaacttttc acgaagataa gcacggacag gcgcttcatg
    26761 acctgagact gcagcaagtt ctgttacttc tttaattttt gaaaataatg ttgtcatttc
    26821 agttccttct ttctttcatc cattttacca ctttttatag gagaaggata gtgggaaggt
    26881 ggatttctaa gttagtatct tagtcctgct ctatcttaga aaaggatagt attctcttgc
    26941 atgtagtgca aaatctagta aacattccaa aattaactcg aatatttatt tccaaacaaa
    27001 aaaacaatac accatcaaag ttgtttagat tttttatgaa atttacagaa aatagttgac
    27061 ttccctttct tctttcttta aat
//
LOCUS       DAVHIU010000015        26670 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVHIU010000015 DAVHIU010000000
VERSION     DAVHIU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.015"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            22..1110
                     /gene="wecB"
                     /locus_tag="VVN06_000545"
     CDS             22..1110
                     /gene="wecB"
                     /locus_tag="VVN06_000545"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5562147.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            1149..2018
                     /gene="rfbA"
                     /locus_tag="VVN06_000546"
     CDS             1149..2018
                     /gene="rfbA"
                     /locus_tag="VVN06_000546"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU5562148.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            2019..2615
                     /locus_tag="VVN06_000547"
     CDS             2019..2615
                     /locus_tag="VVN06_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU5562149.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHRDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            2625..3674
                     /gene="rfbB"
                     /locus_tag="VVN06_000548"
     CDS             2625..3674
                     /gene="rfbB"
                     /locus_tag="VVN06_000548"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5562150.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKTEKE
                     AVEANYAKTQEIITV"
     gene            3740..4591
                     /gene="rfbD"
                     /locus_tag="VVN06_000549"
     CDS             3740..4591
                     /gene="rfbD"
                     /locus_tag="VVN06_000549"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU5562151.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDIIDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFITYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            4654..5739
                     /gene="glf"
                     /locus_tag="VVN06_000550"
                     /pseudo
     CDS             4654..5739
                     /gene="glf"
                     /locus_tag="VVN06_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            6060..8042
                     /locus_tag="VVN06_000551"
     CDS             6060..8042
                     /locus_tag="VVN06_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562152.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            8343..13646
                     /locus_tag="VVN06_000552"
     CDS             8343..13646
                     /locus_tag="VVN06_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5562153.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGSVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPKGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLPTDKEKMYYFNTQTGATTWTLPSDWAESKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FESLDAPAQPGEGLENAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTTTDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(13812..15971)
                     /locus_tag="VVN06_000553"
     CDS             complement(13812..15971)
                     /locus_tag="VVN06_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5562154.1"
                     /translation="MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSE
                     SKLVATTSSKIFDSKNELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVD
                     TIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
                     ATKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDP
                     YSHPEAALDRRNLVLSEMKGQGYISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYL
                     KEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTAS
                     IVHDVPYNYPGTDTPLYNWDHVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKKYGASSEKMAAAYAAFANGGIYHKPMYINKIVFS
                     DGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDE
                     EIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(15968..16564)
                     /gene="recU"
                     /locus_tag="VVN06_000554"
     CDS             complement(15968..16564)
                     /gene="recU"
                     /locus_tag="VVN06_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU5562155.1"
                     /translation="MVNYPHKLSSQKRQPSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KRAIPMKNFHPHQIQHMEQILAQQGICFVLLHFSSQQETYLLPAYDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            16631..17158
                     /locus_tag="VVN06_000555"
     CDS             16631..17158
                     /locus_tag="VVN06_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU5562156.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            17228..17569
                     /gene="gpsB"
                     /locus_tag="VVN06_000556"
     CDS             17228..17569
                     /gene="gpsB"
                     /locus_tag="VVN06_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU5562157.1"
                     /translation="MTSIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEV
                     FGKQILDNTDL"
     gene            17590..17970
                     /gene="rnpB"
                     /locus_tag="VVN06_000557"
     ncRNA           17590..17970
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVN06_000557"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            18055..19212
                     /locus_tag="VVN06_000558"
     CDS             18055..19212
                     /locus_tag="VVN06_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU5562158.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWVSDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYSEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            19225..20619
                     /gene="mapZ"
                     /locus_tag="VVN06_000559"
     CDS             19225..20619
                     /gene="mapZ"
                     /locus_tag="VVN06_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU5562159.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLS
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEAYSEEV
                     PSSEDILPPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLEQEESEKKSKKGF
                     VLTALALVSVIICVSAYYVYRQVNRSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSETSSSSTS
                     QTSSNTTSETSPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            20695..22119
                     /gene="gndA"
                     /locus_tag="VVN06_000560"
     CDS             20695..22119
                     /gene="gndA"
                     /locus_tag="VVN06_000560"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU5562160.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEVS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            22131..22820
                     /locus_tag="VVN06_000561"
     CDS             22131..22820
                     /locus_tag="VVN06_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5562161.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            22918..23880
                     /gene="cbpC"
                     /locus_tag="VVN06_000562"
     CDS             22918..23880
                     /gene="cbpC"
                     /locus_tag="VVN06_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU5562162.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYVVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            23899..24897
                     /gene="cbpJ"
                     /locus_tag="VVN06_000563"
     CDS             23899..24897
                     /gene="cbpJ"
                     /locus_tag="VVN06_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU5562163.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWLVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(25015..26247)
                     /locus_tag="VVN06_000564"
     CDS             complement(25015..26247)
                     /locus_tag="VVN06_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5562164.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDNLHDAMGFRQSLMRVVRIVANLAGV
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKKIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQTYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVTFNTHN"
     gene            complement(26290..>26670)
                     /locus_tag="VVN06_000565"
     CDS             complement(26290..>26670)
                     /locus_tag="VVN06_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU5562165.1"
                     /translation="KKLLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDII
                     SKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLM
                     FQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         8430 a         4721 c         6009 g         7510 t
ORIGIN      
        1 ttatccattt aggaggaaac gatgaaaaag ataatgctag ttttcggtac acgtccagaa
       61 gcaataaaaa tgtgttcatt agtcaatgag ttgagaaaac aggaagatat gaaaacagtt
      121 gtttgtgtaa ctggtcaaca caaggagatg gttagccctg ttttagattt atttggagtt
      181 caaccagact atgatttaga aattatgaag gctaatcaaa acttgttctc tataacaata
      241 agtattttag aaaaaataaa acctgtgtta gaaaaagaac aaccagatat tgttttggtt
      301 cacggtgata ctactacgac atatgcagca gctttagcgg cattttatct aggaattaaa
      361 gttggtcatg ttgaagctgg attacgaact tacaacctgc aaagtccttt cccagaagag
      421 ttcaacagac aatcgacttc tattattgca aattatcatt ttgctcctac agaattagct
      481 aaagaaaatc taataaaaga gggtagaaat aatatctatg ttactggaaa tacagtgatt
      541 gatgcactta caactacagt acaaaaggat tatacgcacc ccgatctaga tttaaacgat
      601 ggaaatcgcc tcatcttatt gactgctcat agacgcgaaa atctcggaga acctatgaga
      661 catatgttta gagctgttaa acgagtttta aatgaatatg acgatgttaa agtaatttat
      721 ccaattcata aaaatccatt ggtacgggaa acagctacag aaatttttgg agatacagaa
      781 cgtattcaaa tcattgaacc tttagatgtt cttgattttc acaactttat gaatcatagt
      841 tatatgattc taactgactc aggaggggtt caagaagagg caccttcttt aggaaagcct
      901 gtattggtca tgcgagatac gacagaaaga cctgaaggag tagctgctgg aacgttgaaa
      961 ttggttggga ctgatgagga gactatttat caaaacttta agatgctttt agacgatccc
     1021 gaagaatata aaaaaatgag tcaagctagt aatccttatg gaaatggtga tgctagtaaa
     1081 cagattgttc gaattttacg tggaatttga gtgtgttcag ataaagtaat atagaaaggt
     1141 accctactat gaaaggtatt attctagcag gtggttcggg gacacgttta tatcctttga
     1201 ctcgcgctgc atcaaaacaa cttatgccgg tttatgataa accgatgatt tactacccac
     1261 tttcaacatt gatgttggct gggattaagg atattttgat tatttccact ccacaggatt
     1321 tacatcgatt ccaagagctt cttcaagacg gatctgagtt tgggctcaaa ctttcttatg
     1381 cagagcaacc aagtccagat ggtttggcac aagcctttat cattggggaa gagtttattt
     1441 ctgatgatag cgttgcgctg atcttaggtg acaatatcta tcacggttct gggctttcca
     1501 agatgctaca aaaggcagcg agtaaggagt cgggagcaac tgtttttggc taccatgtca
     1561 aggatccaga gcgctttggt gtggttgagt ttgatcagga tatgaaggct atttctattg
     1621 aagaaaagcc agagcaacct cgttcaaact atgcagttac aggtctctat ttctatgata
     1681 atgatgtagt agagattgcc aagagtatta aaccaagtcc tcgtggtgaa ctggaaatta
     1741 cagatgtaaa caaggcttac ctagatcgtg gtgatttgtc tgttgagctt atgggacgtg
     1801 gctttgcttg gctggatact ggaactcatg aaagtttact agaggcttca cagtacatcg
     1861 aaacagtgca acggatgcaa aatgttcagg tagcaaactt agaagaaatt gcttaccgta
     1921 tgggctatat cagtcgagaa gatgtattgg ccttagccca accacttaag aaaaatgaat
     1981 acggacagta tctgctccgt ttgattggag aagcatagat gacagataat tttttcggta
     2041 agacgcttgc ggcacgcaag gttgaagcta ttccaggcat gttggagttt gatatccccg
     2101 ttcatagaga taatcgtggc tggtttaaag aaaatttcca aaaggaaaaa atgcttccac
     2161 ttggatttcc agagtctttc tttgcagaag gaaaattgca aaacaatgta tccttctcac
     2221 gtaaaaatgt ccttcgaggt ctccacgcag agccttggga taagtacatc tctgtagcag
     2281 atggagggaa agttctgggt tcttgggttg atctacgcga gggtgaaacc tttgggaata
     2341 cctatcagac agtgattgat gcgagtaagg gaatctttgt tcctcgaggc gtagctaatg
     2401 gcttccaagt tttatcagat acagtgtcat atagctatct ggtcaatgat tactgggctc
     2461 ttgaactcaa acccaagtat gcctttgtga actacgctga tccaagcctt ggtattgaat
     2521 gggaaaatat tgcagaagca gaggtttcag aagcagacaa acatcatcca ctacttaagg
     2581 atgtaaaacc tttgaaaaaa gaagatttgg aataaggaaa gaatatgact gaatacaaaa
     2641 atattatcgt gacaggtgga gctggcttta tcggttctaa ctttgtccat tatgtttacg
     2701 agaactttcc agatgttcat gtgacagtcc tagataagtt gacttatgct ggaaaccgcg
     2761 cgaatattga ggaaatttta ggtaatcgtg ttgagttagt tgttggtgac attgctgatg
     2821 cggagttggt agacaagttg gctgctcaag cagatgctat cgttcattat gcagcggaaa
     2881 gccacaatga taattcgctc aatgatccat cgccatttat tcatactaac ttcatcggaa
     2941 cctatactct tttagaagct gctcgtaagt atgatattcg cttccaccat gtatcgacag
     3001 atgaagttta tggggatctc cctttacgcg aagatttgcc aggtcatggt gaaggaccag
     3061 gtgagaaatt tactgctgaa acaaaataca acccaagctc tccgtactca tcaaccaagg
     3121 cagcctcaga tttgattgtc aaagcctggg tgcgttcttt tggagtcaag gcaacgattt
     3181 ccaactgttc aaataactac ggtccttatc aacatatcga aaaattcatc ccacgtcaga
     3241 ttactaacat cctaagtggt atcaagccaa aactttacgg tgaaggtaag aacgttcgtg
     3301 attggattca taccaatgac cattcttcag gagtttggac aatcttgaca aaagggcaaa
     3361 tcggtgaaac ctacttgatt ggggctgatg gtgagaagaa caataaggaa gttttggaac
     3421 ttatccttaa ggaaatggga caagctgcgg atgcctatga tcatgtgact gaccgtgcag
     3481 gacatgacct tcgctatgcg attgatgcta gcaagctccg tgatgagttg gggtggaaac
     3541 ctgaatttac caactttgaa gctgggctca aggcaacaat caagtggtat acagataacc
     3601 aagaatggtg gaaaacagag aaagaagctg ttgaagccaa ttatgctaag actcaggaga
     3661 ttattacagt ataaaaagca ggaaatagct gctttttatt gctatattgg gaagagttac
     3721 atattagaaa ggtctagaga tgattttaat tacaggggca aatggccaat taggaacgga
     3781 acttcgctat ttattggatg aacgtaatga agaatacgtg gcagtagatg tggctgagat
     3841 ggacattatc gatgcagaaa tggttgagaa agtttttgaa gaggtgaaac cgactttagt
     3901 ctaccactgt gcagcctaca ccgctgttga tgcagcagag gatgaaggaa aagagttgga
     3961 cttcgccatc aatgtgacgg ggacaaaaaa tgtcgcaaaa gcatctgaaa agcatggtgc
     4021 aactctagtt tatatttcta cggactatgt ctttgacggt aagaaaccag ttggacaaga
     4081 gtgggaagtt gatgaccgac cagatccaca gacagaatat gggcgtacta agcgtatggg
     4141 ggaagagtta gttgagaagc atgtgtctaa tttctatatt atccgtactg cctgggtatt
     4201 tggaaattat ggcaaaaact tcgtttttac catgcaaaat cttgcgaaaa ctcataagac
     4261 tttaacagtt gtaaatgacc agtacggtcg tccgacttgg actcgtacct tggctgagtt
     4321 cataacctac ctagctgaaa atcgtaagga atttggttat tatcatttgt caaatgatgc
     4381 gacagaagac acaacatggt atgattttgc agttgaaatt ttgaaagata cagatgtcga
     4441 agtcaagcca gtagattcca gtcaatttcc agccaaagct aaacgtccgc taaactcaac
     4501 gatgagcctg gccaaagcca aagctactgg atttgttatt ccaacttggc aagatgcatt
     4561 gcaagaattt tacaaacaag aagtgagata agtagtagaa tgattttcta gtctaataaa
     4621 agaggcagat aatgaactcc aaaggagctt aagatgtacg attatcttgt tgtggtgctg
     4681 gtctctttgg cgcagtcttt gcccatgaag cagccttaaa aggaaaaaaa gtaaaagtta
     4741 ttgaaaaacg aaatcatatc gcgggtaata tctatactcg tgaagaggaa ggaattcaag
     4801 tttatcaata tggtgctcat atcttccata cttctgataa ggagatctgg gattatgtaa
     4861 atcagtttgc agggtttaac cgttacacca attctcctgt tgcaaactat aagggtgaga
     4921 tttataacct tccttttaat atgaatacct tcaataaact ctggggagtt gtgacgccag
     4981 cagaagcaca agctaagatt gatgaacaac gtgctatttt aaatggtaaa actcctgaaa
     5041 atttggaaga acaggcgatt tctcttgtag gtacagacat ctacgaaaaa ttaatcaaag
     5101 actatacaga gaaacagtgg ggcaaaccaa ctactgaact tccagccttt attattgttt
     5161 gccagtacgc ctgacctatg ataacaacta ttttaacgat acctatcaag ggattccaat
     5221 tggtggatac actcaaatag ttgaaaaatg ttggatcatg aaaatattga tgtagaaaca
     5281 aatgttgatt tctttgtgaa taaagagcaa tatctgaaag attttcctaa gattgtcttt
     5341 actggtatga ttgatgaatt ctttgactat aagttgggcg aactagagta ccgtagtctt
     5401 cgttttgaaa atgagacctt ggatatggaa aattaccaag gaaatgcagt tgtgaactat
     5461 acggatgcag aaatcccata tactcgcatt attgaacaca aacattttga gtttgggagt
     5521 caagcaaaga ctatcattac taaaacatgg gaaaaaggtg atgagcctta ttatccagtt
     5581 aataatgatc gtaataatca tttgtataaa tcgtataaaa aacttgctga tgagcaaggg
     5641 aatgttatct ttggtggccg cttaggacac tatcgttatt acgatatgca ccaagtaatt
     5701 ggagcagctt tgcagtgcgt gagaaatgag ttagattaat actcaatgaa aatcaaagag
     5761 caaactagga agctagccac aggttgctca aaatactgtt ttgaggttgc agatggaagc
     5821 tgacgcggtt tgaagagatt ttcgaagagt ataaacaagt aaaactgact accagttatt
     5881 atttagaaat agtattaaaa attccttgac tatgtgatat agttgaggga tttttaaatg
     5941 atattcatat tttttgcaaa gatgttgttt gaaaaataat tttcaaaaat tctgaaaatt
     6001 ctgttgacat ctttctgaaa agagtctata atggagagaa agttttaaag gagaaaatga
     6061 tgaaaagttc aaaactattt gcccttgcgg gcgtgacatt attggcggcg actactttag
     6121 ctgcatgctc tggatcaggt tcaagcacta aaggtgagaa gacattctca tacatttatg
     6181 agacagaccc tgataacctc aactatttga caactgctaa ggctgcgaca gcaaatatta
     6241 ccagtaacgt ggttgatggt ttgctagaaa atgatcgcta cgggaacttt gtgccgtcta
     6301 tggctgagga ttggtctgta tccaaggatg gattgactta cacttatact atccgtaagg
     6361 atgcaaaatg gtatacttct gaaggtgaag aatacgcggc agtcaaagct caagactttg
     6421 taacaggact aaaatatgct gctgataaaa aatcagatgc tctttacctt gttcaagaat
     6481 caatcaaagg gttggatgcc tatgtaaaag gggaaatcaa agatttctca caagtaggaa
     6541 ttaaggctct ggatgatcag acagttcagt acactttgaa caaacctgaa agtttttgga
     6601 actcaaaaac aaccatgggt gtgcttgcgc cagttaatga agagtttttg aactcaaaag
     6661 gggatgattt tgccaaagct acggatccaa gtagtctctt gtataatgga ccttatttgt
     6721 tgaaatccat tgtgaccaaa tcttctgttg aatttgcgaa aaatccgaac tactgggata
     6781 aggacaatgt gcatattgac aaagttaaat tgtcattctg ggatggtcaa gataccagca
     6841 aacctgcaga aaactttaaa gatggtagcc ttacagcagc tcgtctctat ccaacaagtg
     6901 caagtttcgc agagcttgag aagagtatga aggacaatat tgtctatact caacaagact
     6961 ctattacgta tctagttggt acaaatattg accgtcagtc ctataaatac acatctaaga
     7021 ccagcgacga acaaaaggca tcgactaaaa aggctctctt aaacaaggat ttccgtcagg
     7081 ctattgcctt tggttttgat cgtacagcct atgcctctca gttgaatgga caaactggag
     7141 caagcaaaat cttacgtaat atctttgttc caccaacatt tgttcaagca gatggtaaaa
     7201 actttggcga tatggtcaaa gagaaattgg ttacttatgg ggatgaatgg aaggatgtta
     7261 atcttgcaga ttctcaggat ggtctttaca atccagaaaa agccaaggct gaatttgcta
     7321 aagctaaatc agccttacaa gcagaaggtg tgacattccc aattcatttg gatatgccag
     7381 ttgaccaaac agcaactaca aaagttcagc gcgtccaatc tatgaaacaa tccttggaag
     7441 caactttagg agcggataat gtagtcattg atattcaaca actacaaaaa gacgaagtaa
     7501 acaatattac atattttgct gaaaatgctg ctggcgaaga ctgggattta tcagataatg
     7561 tcggttgggg tccagacttt gccgatccat caacctacct tgatatcatc aaaccatctg
     7621 taggagaaag tactaaaaca tatttagggt ttgactcagg ggaagataat gtagctgcta
     7681 aaaaagtagg tctatatgac tacgaaaaat tggttactga ggctggtgat gagactacag
     7741 atgttgctaa acgctatgat aaatacgctg cagcccaagc ttggttgaca gatagtgctt
     7801 tgattattcc aactacatct cgtacagggc gcccaatctt gtctaagatg gtaccattta
     7861 caataccatt tgcattgtca ggaaataaag gtacaagtga accagtcttg tataaatact
     7921 tggaacttca agacaaggca gtcactgtag atgaatacca aaaagctcag gaaaaatgga
     7981 tgaaagaaaa agaagagtct aataaaaagg ctcaagaaga tctcgcaaaa catgtgaaat
     8041 aactgttgca aaatataaga aaggatttag tatttctctt gaatgctgaa tcctttttta
     8101 catttgtaaa gaaagattct aaatgtactg acccccaaaa gttggagcct ctttttgtca
     8161 gaatagagaa aatttttgtt aattttactt gtttcctatt gctttctcag ctattatttg
     8221 ttatattaaa agtataatta ttttttattt atcagggtta agcattgcac tttcagagga
     8281 aggagtattt tttaaaaagg aaatgtaaac gcttgctaaa aaatgaaagg atttagaagt
     8341 ttatgaataa aggattattt gaaaaacgtt gtaaatatag tattcggaaa ttttcattag
     8401 gtgttgcttc tgttatgatt ggagctgcat tctttgggac aagtccggtt cttgcagata
     8461 gcgtgcagtc tggttccacg gcgaacttac cagctgattt agctactgct cttgcaacag
     8521 caaaagagaa tgatggacgt gattttgaag cgcctaaggt gggagaagac caaggttctc
     8581 cagaagttac agatggacct aagacagaag aagaactatt agcacttgaa aaagaaaaac
     8641 cggctgaaga aaaaccaaaa gaggataaac ctgcagctgc taaacctgaa acacctaaga
     8701 cggtaacccc tgaatggcaa acggtagaga aaaaagaaca aaagggaaca gtcactatcc
     8761 gagaagaaaa aggtgtccgc tacaaccaac tatcctcaac tgctcaaaat gataacgcag
     8821 gtaaaccagc cctgtttgaa aagaagggct tgaccgttga tgccaatgga aatgcaactg
     8881 ttgatttaac cttcaaagat gattctgaaa agggcaaatc acgctttggt gtcttcctga
     8941 aatttaaaga taccaataat aatgtttttg tcggttatga caaggatggc tggttctggg
     9001 agtataaatc tccaacaact agcacttggt atagaggtag tcgtgttgct gctcctgaaa
     9061 caggatcaac aaaccgtctc tctatcactc tcaagtcaga cggtcagcta aatgccagca
     9121 ataacgatgt caatctcttt gacacagtga ctctaccagc tgcggtcaat gaccatctta
     9181 aaaatgagaa gaagatcctt ctcaaggcgg gctcttatgg caatgaccgt acggttgtca
     9241 gcgttaaaac agacaatcaa gaaggcgtaa aagcggatga tactcctgcc cagaaagaaa
     9301 caggttcggt tgttgacgat agcaaggtga cttatgatac gatccagtct aaggtactta
     9361 aggcagtaat tgaccaagcc ttccctcgtg tcaaggaata caccttgaat ggacatactt
     9421 tgccaggaca ggtgcaacag ttcaaccaag tctttatcaa taaccaccga atcacccctg
     9481 aagtcactta taagaaaatc aatgagacaa cagcagagta cttgatgaag attcgcgatg
     9541 atgctcactt aatcaatgcg gaaatgacag tacgcttgca agttgtggac aatcaattgc
     9601 actttgatgt gaccaagatt gtcaaccaca atcaagtcac tccaggtcaa aagattgatg
     9661 acgaaagaaa actactttct tctattagtt tcctcggcaa tgctttagtc tctgtttcta
     9721 gtgatcaaac tggtgctaag tttgatgggg caaccatgtc aaacaatacg catgtcagcg
     9781 gagatgatca tatcgatgta accaatccaa tgaaggattt ggctaagggt tacatgtatg
     9841 gatttgtttc tacagataag cttgctgctg gtgtttggag taactctcaa aacagctatg
     9901 gtggtggttc gaatgactgg actcgtttga cagcttataa agaaacagtc gggaatgcca
     9961 actatgtagg aatccacagc tctgaatggc aatgggaaaa agcttataag ggcattgttt
    10021 tcccagaata cacgaaggaa cttccaaggg ctaaggttgt tatcactgaa gatgccaatg
    10081 cagacaagaa agtcgattgg caggatggtg ccattgctta tcgtagcatt atgaacaacc
    10141 ctaaaggttg ggaaaaagtt aaggatatca cagcttaccg tatcgcgatg aactttggtt
    10201 ctcaagcaca aaacccattc cttatgacct tggatggtat caagaaaatc aatctccaca
    10261 cagatggtct tgggcaaggt gttctcctta aaggatatgg tagtgaaggt cacgactctg
    10321 gtcacttgaa ctatgctgat attggtaagc gtatcggtgg tgtcgaagac ttcaagaccc
    10381 taattgagaa ggctaagaaa tatggagctc atctaggtat ccacgttaac gcttcagaaa
    10441 cttatcctga gtctaaatac ttcaatgaaa aaattctccg taagaatcca gatggaagct
    10501 atagctatgg ttggaactgg ctagatcaag gtatcaacat tgatgctgcc tatgacctag
    10561 ctcatggtcg tttggcacgt tgggaagatt tgaagaaaaa acttggtgac ggtctcgact
    10621 ttatctatgt ggacgtttgg ggtaatggtc aatcaggtga taacggtgcc tgggctaccc
    10681 acgttcttgc taaagaaatt aacaaacaag gctggcgctt tgcgatcgag tggggccatg
    10741 gtggtgagta cgactctacc ttccatcact gggcagctga cttgacctac ggtggctaca
    10801 ccaataaagg tatcaacagt gccatcaccc gctttatccg taaccaccaa aaagatgctt
    10861 gggtagggga ctacagaagt tatggtggtg cagccaacta tccactgcta ggtggctaca
    10921 gcatgaaaga ctttgaaggc tggcaaggaa gaagtgacta caatggctat gtaaccaact
    10981 tatttgccca tgacgtcatg actaagtact tccaacactt cactgtaagt aaatgggaaa
    11041 atggtacacc ggtgactatg accgataacg gtagcaccta taaatggact ccagaaatgc
    11101 gagtggaatt ggtagatgct gacaataata aagtagttgt aactcgtaag tcaaatgatg
    11161 tcaatagccc acaatatcgc gaacgtacag taacgctcaa cggacgtgtc atccaagatg
    11221 gttcagctta cttgactcct tggaactggg atgcgaatgg taagaaactt cctactgata
    11281 aggaaaagat gtactacttc aatacgcaga ctggtgcaac aacttggacc cttccaagcg
    11341 attgggcaga gagcaaggtt tacctttaca agctaactga ccaaggtaag acagaagagc
    11401 aggaactaac tgtaaaagat ggtaaaatta ccctagatct tctagcaaat caaccatacg
    11461 ttctctatcg ttcgaaacaa accaatcctg aaatgtcatg gagtgaaggc atgcacatct
    11521 atgaccaagg atttaatagc ggtaccttga aacattggac catttcaggc gatgcttcta
    11581 aggcagaaat tgtcaagtct caaggggcaa acgatatgct tcgtattcaa ggaaacaaag
    11641 aaaaagttag tctcactcag aaattaactg gcttgaaacc aaataccaag tatgccgttt
    11701 atgtcggtgt cgataaccgt agtaatgcca aggcaagtat cactgtgaat actggtgaaa
    11761 aagaagtgac tacttatacc aataagtctc tcgcgctcaa ctatgttaag gcctacgccc
    11821 acaatacacg tcgtgataat gctacagttg atgatacaag ttacttccaa aacatgtacg
    11881 ccttctttac aactggatcg gacgtctcaa atgttactct gacattgagt cgtgaagctg
    11941 gtgatcaagc aacttacttt gatgaaattc gtacctttga aaacaattca agcatgtacg
    12001 gagacaagca tgatacaggt aaaggcacct tcaagcaaga ctttgaaaat gttgctcagg
    12061 gtatcttccc atttgtagtg ggtggtgtcg aaggtgttga agataaccgc actcacttgt
    12121 ctgaaaaaca cgatccatat acacaacgtg gttggaatgg taagaaagtc gatgatgtta
    12181 tcgaaggaaa ttggtcactc aagacaaatg gactagtgag ccgtcgtaac ttggtttacc
    12241 aaactattcc gcaaaacttc cgttttgaag caggtaagac ctaccgtgta acctttgaat
    12301 acgaagcagg ttcagacaat acctatgctt ttgtagtcgg taagggagaa ttccagtcag
    12361 gtcgtcgtgg cactcaagca agcaacttgg aaatgcatga attgccaaat acttggacag
    12421 attctaagaa agccaagaag gcaaccttcc ttgtgacagg tgcagaaaca ggagatactt
    12481 gggtaggtat ctactcaact ggaaatgcaa gtaatactcg tggtgattct ggtggaaatg
    12541 ccaacttccg tggttataac gacttcatga tggataatct tcaaatcgaa gaaattaccc
    12601 taacaggtaa gatgttgaca gaaaatgctc tgaagaacta cttgccaacg gttgccatga
    12661 ctaactacac caaagagtct atggatgctt tgaaagaggc ggtctttaac ctcagtcagg
    12721 ccgatgatga tatcagtgtg gaagaagcgc gtgcagagat tgccaagatt gaagccttga
    12781 agaatgcttt ggttcagaag aaaacggctt tggtagcaga tgactttgaa agtttggatg
    12841 cgccagctca accaggtgaa ggtcttgaga atgcctttga tggaaactta tctagtttat
    12901 ggcatacatc atggggcgga ggagatgtag gcaagcctgc aaccatggtc ttgaaagaac
    12961 caactgaaat cactggactt cgttatgttc cacgtggatc aggttcaaat ggtaacttgc
    13021 gtgatgtgaa acttgttgtg acagatgagt ctggcaagga gcataccttt actacaactg
    13081 attggccaga taacaataag tcaaaagata ttgactttgg taagacaatc aaggctaaga
    13141 aaattgtcct tactggtacc aagacatacg gagatggtgg agataaatac caatctgcag
    13201 cggaacttat ctttactcgt ccacaggtag cagaaacacc tcttgacttg tcaggctatg
    13261 aagcagcttt ggctaaggct cagaaattaa cagacaaaga caatcaagag gaagtagcta
    13321 gcgttcaggc aagcatgaaa tatgcgacgg ataaccatct cttgacggaa agaatggtgg
    13381 aatactttgc agattatctc aaccaattaa aagattctgc tacgaaacca gatgctccaa
    13441 ctgtagagaa acctgagttt aaacttagct ctttagcttc cgatcaaggt aagacgccag
    13501 attataagca agaaatagat agaccagaaa cacctgaaca aatcttgcca gcaacaggtg
    13561 agagtcaatc tgacacagcc ctcttcctag caggtgttag cctagcccta tctgctctct
    13621 ttgtagtaaa aacgaagaaa gactagtatt tagtaaaacc tcttaacaag attacggaag
    13681 cagtctctat cttttccaat gaggtttata gtacagaaaa aatctgagaa gatgtcttct
    13741 cagacttttg ttaagcacat aaatacaata gtgctatgac aaaatcaccc agaaaatctg
    13801 ggtgataaat gttatggttg tgctggttga ggattctgat tttgttgatc aggggttgta
    13861 tttgattgtt gcgtattatt gttaggattg gtagtcgtac tattatttgt gcttggagtg
    13921 gttgagctag actgtgaagt tgaactatct gatgatgagc ttgaactttc agttgatggg
    13981 ggttgttgtg gagcaggtga gctccacgta gaacgagcac catttttaaa tacgaattct
    14041 ccatttctgt agagcccctc tggtatattc caatcctctg gattgcttcc ttcagacagg
    14101 taggtcatca tagagcgata aactttagct gcaactagga aaccatctcc aacgatagga
    14161 gttaaacgat tcgaataacc tgtccataca gccatagaat acttacgagt ataacccaca
    14221 aacatttcat ctggagctac gtagccagtg ttcttgatat acttttcaat ttcttcgtca
    14281 gtatagttag aagtacctgt cttacctgct tgtggaagcc atggtaggta ggctccacgt
    14341 cctgttccgt aagttaaaac agttttcatc atttcagtca tcatataggc agtagtctct
    14401 ttcatagctc gtgtaccagc atcagaaaat tctttttcgc taccatcact aaagacgatt
    14461 ttattgatat acattggttt gtgataaata ccaccattag caaaagcagc gtaggcagca
    14521 gccatttttt cactacttgc accatatttt ttgttggatt cagttgtgtt acttgaaatg
    14581 gcgtttgcat aatgcatgct tggatagtcg ataccaagac cattaaggaa ggttttagct
    14641 ctatctagac cgaccttatt caaagtctca acggctgtga catttcgtga ttgttgaaga
    14701 gcatactgga ttgtaatgtt tccaaagtag acatgatccc agttgtagag tggagtatca
    14761 gtgccaggat agttataagg gacatcatgt acaatagaag cagtagagtc atagactcca
    14821 tattctaaag cgggagcata gtcagtgatt ggtttcattg atgatcccca gtcacgattg
    14881 gtttctacgg cctggttggt accgaatgaa acattacttg cttgatgacg agcaccaagt
    14941 tgtgcgatga ctttaccatt tgaaacatct acgaccgtag atgcgacttg caaatcatcg
    15001 tcagggtaag agacgtattg atcggagttg tagatatccc acagatgttt ttgagcttct
    15061 tggtctacat ttgtgtaaac atccatccca gtagttagaa ggttatagcc agtttcttgt
    15121 tctacttggt cgataacctc tttgagataa ttgtccatat atgctggata gctattgacc
    15181 gatttcaaac tttggagtcc atcagtaata ggggtattaa tagccttctc atactgttcg
    15241 gcagaaatgt agccttgacc tttcatctct gaaagtacca agttacgacg gtcaagggca
    15301 gcttctggat gagaataggg atcgtattga ttcggagcct gtggcattcc tgctagtagg
    15361 gcaagttgag gtaatgatag ttctcgcaaa tctttgccgt aatagttttg ggcagccgtc
    15421 tgcattccat agtttccgtt agacatgtaa actttattca catagtaggt caggatttct
    15481 tgttttgtgg ctttttgttc taattgaaca gccaaccaag cttcctgggc tttacgtgaa
    15541 atggtctgat cagaagtgga agttgagaaa taggtcaact taattaattg ttgggtaagg
    15601 gtagatcctc cctggaggga gttgttttgt aagttacgta aaaaagctcc tataattcgg
    15661 atggtatcga caccgcgatg atcgaaaaaa cgatggtctt cgatcgatac gatagccttg
    15721 accaaatcag taggaatttc gttggcctga gcgtttactc ggcgttcaga gcccagatcg
    15781 gcaatcagtt cgtttttact atcgaaaatc ttactagacg tggttgcgac taatttgctc
    15841 tcagataggg ctggggcttt gctgacgtaa tagagaaata tgcctcctcc cagcataact
    15901 gctgtgataa ataaagttaa gaagcagata ctcacatact tagctattcg caggatagtt
    15961 tgtttgttca tcttgtttta ccacctaata aatgttcttt gataacattg agatagggaa
    16021 tttgagggaa ggcaccagcc ttgatttcat atccatattc tcgaatatat ccaagtggca
    16081 ttgatttttg ccccttatct tgatgataga agcgaatcaa atcatatgcc ggtaataagt
    16141 aggtttcttg ctgagaagaa aagtgaagaa ggacaaagca gattccttgt tgagcaagga
    16201 tttgttccat atgctgaatc tgatgtggat ggaaattttt catcggaatc gcacgttttt
    16261 gttttgtttc cttggcttca aagtcgatgt aatatccatt ataaacgcca gaatagtccg
    16321 tcgttgaagc ttgtcgaaaa taggcttcaa caatcttggc acgacttcgt tgtggatagt
    16381 ccactcgtac gatttgaata ggagttggtt tcttgtgtat aacagccaag ccctgagaca
    16441 aatagtagtc gttggtagca ttgatcatct tttcaaaaga cattcctcga tttgcgaaat
    16501 ttttgggttg agaaagagat ggttgtcttt tttgtgatga aagtttatgt ggatagttga
    16561 ccataattct ccttattggt acaataacat cactctatta taccataaat ttgcacagaa
    16621 agggttaaaa atggctacag ctttggtttt gggctattct gcttttgatt tgggtttatt
    16681 ttctgataag gatcctcgtc ttaaattgat aaaaaaagca atacgtaaag atttagaggc
    16741 tatggcagca gatggggtgt cttggcttgt gtttacaggg actttgggct ttgaatattg
    16801 ggttttagag gttgctcagg agatgaaaac cgagtacggt ttccagttgg ccaccatttt
    16861 tgcttttgaa acccatgggg aaaattggaa tgaaggcaat cagatgaaac tgagtcgttt
    16921 taagcaggta gactttgtca aatatgctta tcctcgctat gagcacaagg ggcagttaag
    16981 agattaccag cagtttttgc tggaaaacac gactagttcc tatctttttt atgatgaaga
    17041 aaatgaaacg aaactagctt atttttacca aaagatgaaa aatcaagagg actattttat
    17101 aaagagatta acatttgatc aattaaatga acttgctgaa aatttttccg aaaattgaag
    17161 ctttgacctt gatttttgtt tgcctttttt tatataataa tactagcaag cgagaatgga
    17221 gagagacatg acaagtatta ttttttcagc aaaagatatt tttgaacaag agtttggacg
    17281 tgaagttcgt gggtatagca aggtagaagt tgatgagttt ttagacgatg tcatcaagga
    17341 ctatgaaacc tatgctgcct tggttaagtc acttcgtcag gagattgcgg atttgaagga
    17401 agaattaact cgtaaaccgc aagtgagttc ggctccaagt cctagtcacc cagatccaat
    17461 tgatgtggct gcttcatctt ctatgacgaa ttttgatatt ttgaaacgct tgaatcgtct
    17521 tgaaaaagaa gtattcggta aacaaatttt agacaatact gatttgtaat tcaggtcttg
    17581 ttacatgcaa tttttggata atcgcgtgag gagaattgct tctcatgagg aaagtccatg
    17641 ctagcacagg ctgtgatgcc tgtagtgttt gtgctaggcg aaaccataag cctagggacg
    17701 agaaatcgtt acggcagttg aaatggctaa gtccttggat aggccagagt aggcttgaaa
    17761 gtgccacagt gacggagtct ttctggaaac agagagagtg gaacgcggta aacccctcaa
    17821 gctagcaacc caaattttgg tcggggcatg gagtacgcgg aaacgaacgt agtattctga
    17881 ctgctatcag ctagagctgc tagtggtaga cagatgatta tcgaaggaag tggtcctagt
    17941 catttctgga acaaaacatg gcttatagaa aattgcatat aggttggggc tgagaaattt
    18001 tctcaacctc attttttaaa gtggacatat agaaaggtct tgcaagactg taacatgaaa
    18061 aaagaattta atttaattgc aactgtggca gcaggtcttg aagctgttgt aggacgagaa
    18121 gtaagagagc tgggctacga ttgtcaggtt gaaaatgggc gtgtgcgttt tcaaggagac
    18181 gtgagagcga ttatcgaaac caacctctgg cttcgggcag cagatcgtat caagatcatc
    18241 gtaggaacgt tcccagctaa gacgtttgaa gagttgtttc agggagtttt tgctttagat
    18301 tgggaaaatt atttaccact cggagctcgg ttcccaattt caaaagccaa atgtgttaaa
    18361 tctaaacttc acaatgaacc cagtgttcag gctatttcta agaaagctgt tgtcaagaaa
    18421 ttgcagaaac actatgctcg cccagaaggg gttcctctga tggagaatgg cccagagttt
    18481 aagattgagg tctctattct caaagatgtg gcaactgtca tgattgatac gacaggctct
    18541 agcctcttta aacgtggtta tcgtaccgaa aaagggggag cccctatcaa ggaaaacatg
    18601 gcggcagcca ttttacaact ttctaactgg tatccagaca agcctttgat tgatccgaca
    18661 tgtggttcgg ggactttctg tatcgaggcg gtcatgattg ctcgaaagat ggctccaggt
    18721 cttcgtcgct cctttgcttt tgaggaatgg aattgggtca gcgatcgctt gattcaggaa
    18781 gtgcgtacag aagcggctaa aaaagtagac cgtgagcttg aactggatat tatgggctgt
    18841 gatatcgatg cacgtatggt ggaaattgct aaggccaatg ctcaggcagc tggtgttgca
    18901 ggagacatta cttttaagca gatgcgcgtt caggatttac gttctgataa aattaatggt
    18961 gtaatcattt ccaatccgcc ttatggtgaa cgtttatcag atgatgcagg agttaccaag
    19021 ctctattctg agatggggca agtatttgca ccactgaaaa cttggagcaa atttatcctg
    19081 actagtgatg aagcctttga aagcaagtat ggtagccaag cagataagaa gcgtaagtta
    19141 tacaacggaa ccttgaaagt ggatctgtat caatattttg gtcagcgtgt caaacggcaa
    19201 gaggtaaaat agaaagggat actcatgagt aaaaaaagac gaaatcgtca taaaaaagaa
    19261 ggtcaagaac cgcaatttga ttttgatgaa gcaaaagagc taacagttgg tcaagcgatt
    19321 cgtaaaaatg aagaagtgga agcaggagtc ttgtctgagg attccatttt ggacaagtat
    19381 gtcaagcaac acagagatga aattgaggcg gataagtttg cgactcgtca atacaaaaaa
    19441 gaggagttag tcgaaactca gagtctggat gatttaattc aagagatgcg tgaggctgta
    19501 gaagagtcag aagcttattc ggaggaagtt ccatcttctg aagacatctt accacccttg
    19561 cctctggatg atgaggagca aggcttggat cctctattac tagaggatga aaatccaaca
    19621 gaaatgactg aagaagtgga agaggagcaa aatctttctc gtctggaaca agaagagtca
    19681 gaaaagaaaa gcaaaaaagg ctttgtttta accgctttgg cgcttgtatc agtgattatc
    19741 tgtgtcagtg cttattatgt ctatcgtcaa gtgaatcgct cgaccaagga aattgaaact
    19801 tctcaatcaa ctacagccaa tcaatcggat gtggatgatt ttaatacact ttatgacgcc
    19861 ttctacacag acagtaataa aacggctttg aaaaatagcc agtttgataa actaagtcaa
    19921 ctcaagacct tacttgataa gctggaaggt agtcgtgaac atacgcttgc caaatctaaa
    19981 tatgatagtc tagcaacgca aatcaaggcc attcaagatg tcaatgcaca atttgaaaaa
    20041 ccagctattg tggatggtgt gttggatacc aatgccaaag ccaaatcgga tgctaaattt
    20101 acagatatta aaactggaaa tacggagctt gataaagtgc tagataaggc tatcagcctt
    20161 ggtaagagcc agcaaacaag tacttctagc tcaagttcaa gtgaaactag tagctcaagc
    20221 actagtcaaa caagctcaaa tacgactagt gagacaagcc caagtagttc aaatgagact
    20281 agaagtagtc gcagtgaagt caatatgggt ctctcgagtg caggggttgc tgttcaaaga
    20341 agtgccagtc gtgttgccta taatcagtct gctattgatg atagtaacaa ctctgcctgg
    20401 gattttgcgg atggtgtctt ggaacaaatt ctagctactt cacgttcacg tggctatatc
    20461 actggagacc aatatatcct tgaacgtgtc aatatcgtta atggcaatgg ttattacaac
    20521 ctctacaagc cagatggaac ctatctcttt acccttaact gtaagacagg ctactttgtc
    20581 ggaaatggcg ctggtcacgc ggatgacttg gactactaag cagtcgttac aaaattcttt
    20641 cttttcaaaa gtaaaaatga taaaataaaa caaatgaaac aagaggagtg tcaaatgaca
    20701 aaagctaact ttggtgtcgt aggtatggcc gtaatgggtc gtaaccttgc ccttaatatt
    20761 gaatctcgtg gttacacagt tgctatttac aaccgtagta aagaaaaaac tgaagatgta
    20821 attgcttgcc atcctgaaaa gaactttgta ccaagctatg acgttgaaag ttttgtaaac
    20881 tcaatcgaaa aacctcgtcg tatcatgctg atggttcaag ctggacctgg tacagatgct
    20941 actatccaag cccttcttcc gcaccttgac aagggtgata tcttgattga cggtggaaat
    21001 actttctaca aagataccat ccgtcgtaat gaagaattgg caaactcagg tatcaacttt
    21061 atcggtactg gagtttctgg tggtgaaaaa ggtgcccttg aaggtccttc tatcatgcct
    21121 ggtggacaaa aagaggccta cgaattggtt gcggatgttc ttgaagaagt ctcagctaaa
    21181 gcaccagaag atggcaaacc atgtgtgact tacatcggcc ctgatggagc tggtcactat
    21241 gtgaaaatgg ttcacaatgg tattgagtac ggtgatatgc aattgatcgc agaaagctat
    21301 gacttgatgc aacacttgct aggcctttct gcagaggata tggctgaaat ctttactgag
    21361 tggaacaagg gtgaattaga cagctatttg atcgaaatca cagctgatat cttgagccgt
    21421 aaagacgatg aaggccaaga tggaccaatc gtagactaca tccttgatgc tgcaggtaac
    21481 aagggaactg gtaaatggac tagccaatca tctcttgacc ttggtgtacc attgtcactg
    21541 attactgagt cagtgtttgc acgctacatt tcaacttaca aggaagaacg tgtacatgct
    21601 agcaaggtgc ttccaaaacc agctgccttc aactttgaag gagacaaggc tgaattgatt
    21661 gaaaaaatcc gtcaagccct ttacttctca aaaatcattt catacgcaca aggatttgct
    21721 caattgcgtg tagcctctaa agaaaacaac tggaacttgc catttgcaga tatcgcatct
    21781 atctggcgtg atggctgtat catccgttct cgtttcttgc aaaagattac agatgcttac
    21841 aaccgtgatg cagaccttgc caaccttctt ttggatgaat acttcttgga tgttactgct
    21901 aagtatcaac aagcagtgcg tgatatcgta gctcttgcgg ttcaagctgg tgtgccagtg
    21961 ccaactttct cagcagctat tacttacttt gatagctacc gttcagctga ccttccagct
    22021 aacttgatcc aagcacaacg tgactacttt ggtgctcaca cttaccaacg taaagacaaa
    22081 gaaggaacct tccactactc ttggtatgac gaaaaataag taggtcagcc atggggaaac
    22141 ggattttatt acttgagaaa gaacgaaatc tagctcattt tttaagtttg gaactccaga
    22201 aagagcagta tcgggttgat ctggtagagg aggggcaaaa agccctctcc atggctcttc
    22261 agacagacta tgatttgatt ttattgaacg ttaatctggg agatatgatg gctcaggatt
    22321 ttgcagaaaa attgagtcga actaaacctg cctcagtcat catgatttta gatcattggg
    22381 aagacttgca agaagagctg gaagttgttc agcgttttgc agtttcatac atctataagc
    22441 cagtccttat cgaaaatctg gtagcgcgta tttcggcgat cttccgaggt cgggacttca
    22501 ttgatcaaca ctgcagtctg atgaaagttc caaggaccta ccgcaatctt aggatagatg
    22561 ttgaacatca cacggtttat cgtggtgaag agatgattgc tctgacccgc cgtgagtatg
    22621 accttttggc gacacttatg ggaagcaaga aagtattgac tcgtgagcaa ttgttggaaa
    22681 gtgtttggaa gtatgaaagt gcgaccgaga caaatatcgt agatgtctat atccgctatc
    22741 tacggagcaa gcttgatgtt aaaggccaaa aaagctacat taaaaccgtg cgtggtgttg
    22801 gttacaccat gcaagaatag aaaagcagtt gcagttgtgt aactgctttt ttgaggagtt
    22861 tttatatatt gacatgcagt cgggtctttg ctacaatcgt tttaggaggg tatatagatg
    22921 aaaattttga aaaaaatgat gcaagttcta ctagcagtct ttttctttgg tttgctagct
    22981 acaaatacgg tatttgcgaa taccacaggt ggccgatttg ttgataagga taatagaaaa
    23041 tattatgtaa aagatgatca taaagcaatc tattggcata aaatagacgg taaaacctac
    23101 tattttggtg atggaggaga gatggttgtc ggttggcaat acttagaaat tcctggaaca
    23161 ggttatcgtg ataatttatt cgataaccaa ccagttaatg aaattggcct tcaggagaag
    23221 tggtactatt ttggacaaga tggtgctttg ttagaacaaa cagataaaca agtactagag
    23281 gcaaaaacgt ctgaaaatac aggaaaagta tacggtgaac aatatcctct atctgctgaa
    23341 aagagaactt attattttga taataattat gttgtaaaga caggctggat ttatgaagac
    23401 ggcaattggt attatttaaa taagctagga aattttggcg atgattctta caatccacta
    23461 ccaattggtg aagttgctaa gggttggact caagattttc atgttactat tgacattgat
    23521 agaagcaaac ctgctccatg gtactaccta gatgcttccg gtaagatgct tacagattgg
    23581 caaaaagtaa acggaaaatg gtattatttt ggctcctctg gttctatggc aacaggttgg
    23641 aaatatgtac gaggcaaatg gtattactta gataataaaa atggtgatat gaagactggt
    23701 tggtatcaag atggttcaac ttggtactat ttagatcctt ctaatggaga tatgaaaata
    23761 ggttggacaa aagtaaatgg aaaatggtat tatctcaatt caaatggagc aatggttaca
    23821 ggtagtcaaa ctatcgatgg taaagtttat aacttcgctt catctggtga gtggatttaa
    23881 tgttggagga tatataaaat gaagcttttg aaaaaaacta tgcaagctgg actaacagta
    23941 attttctttg gtttgctagc tacgaataca gtatttgcag ataactctga aggatggcag
    24001 tttgtccaag aaaacggtag aacctattac aaaaaggggg acctcaaaga aacctactgg
    24061 ctagtgattg atggtaagta ctattatttt gattctctat ctggagagat ggttgtcggc
    24121 tggcaatata tcccgtttcc atctaaaggt agtacaattg gtccttaccc aaatggtatc
    24181 agattagaag gttttccaaa gtcagagtgg tactacttcg ataaaaatgg agtgctacaa
    24241 gagtttgttg gttggaaaac attagagatt aaaactaaag acagtgttgg aagaaagtac
    24301 ggggaaaaac gtgaagattc agaagataaa gaagagaagc gttattatac gaactattac
    24361 tttaatcaaa atcattcttt agagacaggt tggctttatg atcagtctaa ctggtattat
    24421 ctagctaaga cggaaattaa tggagaaaac taccttggtg gtgaaagacg tgcggggtgg
    24481 ataaacgatg attcgacttg gtactattta gatccaacaa ctggtattat gcaaacaggt
    24541 tggcaatatc taggtaataa gtggtactac ctccgttcct caggagcaat ggccactggc
    24601 tggtatcagg aaggtaccac ttggtattat ttagaccagc caaatggcga tatgaaaaca
    24661 ggttggcaaa accttgggaa caaatggtac tatctccgtt cctcaggagc aatggcaact
    24721 ggttggtatc aagatggttc aacttggtac tacctaaatg caggtaatgg agacatgaag
    24781 acaggttggt tccaggtcaa tggcaactgg tactatgctt atagctcagg tgctttggca
    24841 gtgaatacga ccgtagatgg ctattctgtc aactataatg gcgaatgggt tcggtaatga
    24901 cagggcgctt ataattatat tagaacactc gcttaatcaa agaagtgact ttgttgtact
    24961 tgacctgacg attggggaga ttaaaagcat gtgaaatcta cacatgcttt ttcgttagtt
    25021 atgagtgttg aacgtgacta accattgaca aaagataaga gcaattaaaa taagatacaa
    25081 aaatatcaat gctaactgca ccgaaacgcc cgatgctatg gatagggcta atagggagag
    25141 cagggacggg gcgagaatgt ctactgtgct cagagcagag cccaccgttc ccatcttgtc
    25201 actggggata tgggcaaaga cagctgcttg taggcgaggg ctgagaatgc cgataatcgt
    25261 agaactgatg aaactggtca tgaaaattac gatataggtt tgacaaagga tactagttat
    25321 tagagccaat agggaaatct cacagaaaac caagaggtgc gtgaaacgga tatttttaaa
    25381 tagattactg ctggtcatat tgccaaggat agctccacaa gaaatgatga tggagagcag
    25441 ggcgaggagt tgcccaatgc tcagttgtgc tagtgtctgt tggatgaagc ggagggcaat
    25501 cagccgaggg gacacatcca agatggcaat catactggtc gtcagaaaca gtagtatgac
    25561 ggtatctttt aacctcagga gcacagccag cgactgttta agatgttgaa aatatttctt
    25621 aaaactcagt gctgtatgtg acatttcaat ctttttttca acctcataca aggtatgtcg
    25681 aataacatac aggcccaaaa aggcaatgac aaaagtcaga gtgttgataa gggaaatagt
    25741 ttgaatactc ataacattga taaggaatac gccagccaga ttggcgacaa tgcggacaac
    25801 cctcatcaga gactgcctga accccatggc atcatgcagg ttgtccttaa ttacactgat
    25861 gtagagggcg ttcatcatgt aggcagacag gtagctcaag gtgtcagaga tgaggttgat
    25921 gatgacaatc ataatgactg aaactaatga aaagggcgac aagacgagaa atactgtcaa
    25981 aatagcgtag agcaggattt tgattttggc aatacagagt gcgtgtttga ctctattttc
    26041 ttgaaaatct cctaaaatcc cgagaaagag ttgcgatagt aggggaacca tctctgaaat
    26101 ggaaatcagt aaaatcgcta gcggagcgaa attgtattga gccacataat tgataatggc
    26161 gaggtagaaa atactgtcgc cgaatgatga aaaacagctg tttagcagca actgtctata
    26221 tatgttgttt gtccaaaata atttcatact tttctcctta tgtctgcaca tcgaatggga
    26281 atttattatt taatagacac caatataccc catgcttcct attccgaagt caaacaaatc
    26341 tgctttacca ctatctcctt gaaacatcag taaaccgtga tcatctcgtt cagaacatgc
    26401 taaaattacc tgttggattt ttttcatcag taatttgttt tgattgtaga cagtggtttg
    26461 tagtaggcta gttattccgt ggcaatatcc tgtcatcatt tttagatgat ggttaaaaac
    26521 aaattcttga tatttactaa taatatcttt gtttccgtca caatcgtaca tacaaagata
    26581 taagattatt ccggaaattc ctttgcacca agaaagttgt aaatttccta aattttcagt
    26641 attagaagta actttttcta ataatttttt
//
LOCUS       DAVHIU010000016        26603 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVHIU010000016 DAVHIU010000000
VERSION     DAVHIU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.016"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(136..903)
                     /locus_tag="VVN06_000566"
     CDS             complement(136..903)
                     /locus_tag="VVN06_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU5562166.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(900..1766)
                     /locus_tag="VVN06_000567"
     CDS             complement(900..1766)
                     /locus_tag="VVN06_000567"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5562167.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VVN06_000568"
     CDS             complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VVN06_000568"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5562168.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VVN06_000569"
     CDS             complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VVN06_000569"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5562169.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(3601..4086)
                     /locus_tag="VVN06_000570"
     CDS             complement(3601..4086)
                     /locus_tag="VVN06_000570"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5562170.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VVN06_000571"
     CDS             complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VVN06_000571"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5562171.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VVN06_000572"
     CDS             complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VVN06_000572"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5562172.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LSEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VVN06_000573"
     CDS             complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VVN06_000573"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5562173.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VVN06_000574"
     CDS             complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VVN06_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU5562174.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(8117..8341)
                     /locus_tag="VVN06_000575"
     CDS             complement(8117..8341)
                     /locus_tag="VVN06_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5562175.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8401..9375)
                     /locus_tag="VVN06_000576"
     CDS             complement(8401..9375)
                     /locus_tag="VVN06_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU5562176.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9375..9809)
                     /locus_tag="VVN06_000577"
     CDS             complement(9375..9809)
                     /locus_tag="VVN06_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5562177.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGHLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9903..10688)
                     /locus_tag="VVN06_000578"
     CDS             complement(9903..10688)
                     /locus_tag="VVN06_000578"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5562178.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11013..12377
                     /locus_tag="VVN06_000579"
     CDS             11013..12377
                     /locus_tag="VVN06_000579"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5562179.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12380..12751
                     /locus_tag="VVN06_000580"
     CDS             12380..12751
                     /locus_tag="VVN06_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU5562180.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            12965..14239
                     /gene="serS"
                     /locus_tag="VVN06_000581"
     CDS             12965..14239
                     /gene="serS"
                     /locus_tag="VVN06_000581"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5562181.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(14264..15163)
                     /locus_tag="VVN06_000582"
     CDS             complement(14264..15163)
                     /locus_tag="VVN06_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU5562182.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMTQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(15273..15821)
                     /locus_tag="VVN06_000583"
     CDS             complement(15273..15821)
                     /locus_tag="VVN06_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5562183.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16039..17355)
                     /locus_tag="VVN06_000584"
     CDS             complement(16039..17355)
                     /locus_tag="VVN06_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU5562184.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALTQS
                     AFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLMVL
                     LGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(17446..17534)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17698..20034)
                     /locus_tag="VVN06_000585"
     CDS             complement(17698..20034)
                     /locus_tag="VVN06_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU5562185.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHATEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADRGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20138..20686)
                     /locus_tag="VVN06_000586"
     CDS             complement(20138..20686)
                     /locus_tag="VVN06_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5562186.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20683..20985)
                     /gene="zapA"
                     /locus_tag="VVN06_000587"
     CDS             complement(20683..20985)
                     /gene="zapA"
                     /locus_tag="VVN06_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5562187.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21072..21953
                     /locus_tag="VVN06_000588"
     CDS             21072..21953
                     /locus_tag="VVN06_000588"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU5562188.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGNRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            21957..22571
                     /gene="lepB"
                     /locus_tag="VVN06_000589"
     CDS             21957..22571
                     /gene="lepB"
                     /locus_tag="VVN06_000589"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5562189.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22699..25065
                     /locus_tag="VVN06_000590"
     CDS             22699..25065
                     /locus_tag="VVN06_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU5562190.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIVGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEVRDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDTDFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDTEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDHATQHIGTARKTYLVERFSDLLKN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(25113..26396)
                     /locus_tag="VVN06_000591"
     CDS             complement(25113..26396)
                     /locus_tag="VVN06_000591"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5562191.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         7658 a         6160 c         5184 g         7601 t
ORIGIN      
        1 atttttgtaa gcttacatct acagtataca tcttttttgt ctattttata gcccatctcc
       61 tcaactgtca atttttcaac ctgaattgca tttttccata aaaaatgaga cctttctagt
      121 ctcatttagt cattcttagt attttctaaa tcgttgatag cgttcttcca gcaactcttc
      181 tagcggtttt tgtgaaagtc tagccagctc cgtttggagt tcttttttga cactcttaat
      241 cagttcttta ctagaaagtc ctacttcaga aatcacctta tccaccacgt ccatttctaa
      301 cagttcatgc gaagtgattt tcatcagttc tgctgcttcc atggcgcgag taccgtcctt
      361 ccataaaatg gaagcaaagc cttctggact gagaatggca tagatagaat tttccagcat
      421 ccagacacgg tccgcgacag ctagagccag agccccgcct gaaccacctt caccgataat
      481 aatggcgata ataggaactt tcaggtcact catttccatg agattgcgag cgatagcttc
      541 cccttgacca cgttcttccg ctccgacacc aggataagca cctgctgtat tgataaaggt
      601 cacaactgga cggccaaatt tctcagcctg tttcatcaac cgcagtgcct ttcggtagcc
      661 ttctggatgt ggttggccaa aattccgttt gaggttgtct tgcaaactct tgcctttttg
      721 gataccaacc actgttacag cttggtctcc aagccaacca ataccaccaa caactgcacc
      781 atcatcacga aaagaacggt caccatgtaa ttggataaat tcatcaaaaa tgcctgtagc
      841 aaagtccaag gttgtcaagc gactctgctc acgcgcttct ctgactattt ttgcaatatt
      901 catctaggac tccctccatg caatctgact aggctagcaa tcgtatctgg taagtctctt
      961 cttttgacaa tagcatccac aaagccatgt tctaatagga attctgcctt ttggaaatcc
     1021 tcaggcaagc tttcacgaac cgtattttca atcacacgac gtccagcaaa accaaccaag
     1081 ctctgtggtt cagccagaat gatatcgcct tccatagcga aagaagctgt cacaccacca
     1141 gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag catttgaatg gcgtttaacc
     1201 gccgcagaga tcttagccat ctgcatgaga ctcatgattc cttcctgcat acgggctcca
     1261 ccagaggctg tgaataggac aactggcaat ttttcgacag tcgcatactc aaacaaacga
     1321 gtgatttttt cacctacaac cgtacccata gaagccatga taaagttaga atccataatc
     1381 ccaagagcca cagtctgacc tttaataaga gcagttcctg tcacaacggc ttcatgcaga
     1441 cctgtttttt cacgcataga tgccagtttc ttttggtaac cagggaaatg caagggatcc
     1501 ttgctttcaa tccctgtaaa caattctttg aaggttccca tatcaatcgt caaagccaag
     1561 cgttcttggg cagaaatacg aaaggtatag ctacagtgcg gacagatacg ttcacttccc
     1621 agatccttct gatagatggt atgcttacag cctggacact gggaaaataa ttcatctgga
     1681 acctctggct tagcttgagg tttttcccta accgaacgat tgggattgat tcgaatatac
     1741 ttatcttttt tactaaatag agccattgat tccccttttc ggtttaaact cttaaagtca
     1801 ttttattctt tttcttgata tttaggtaag aaggtttcca tcaagaagga agtatcataa
     1861 tccccagcaa tgacattgcg atctgaaatg aggtcaagct ggaaatctgc attggtctgc
     1921 actccttcaa tctctaattc atagagggca cgttgcattt tcatcaaggc gtcaaaacga
     1981 ttttcgccgt gtactatgat tttggcaatc atactatcat aataaggcgg aatggtataa
     2041 cctggataaa ctgctgaatc cacgcgcaag ccaactccac cacttggcag atagagatta
     2101 gtaatcttac ctggacttgg agcaaagtta aaggctgggt tttctgcatt gatacgacac
     2161 tcgatggcat gaccgcgtag gacaatatct tcttgcttaa cagacaaagg ctgacctgcc
     2221 gcaatgcaaa tctgttcctt aacgatatca acacctgaaa caaactctgt tactggatgt
     2281 tctacctgaa cacgagtatt catctccatg aaatagaaat tgctacttgc ttcatcaaga
     2341 agaaattcaa tggttcctgc attctcatag ccaacaaact ctgccgctcg aacagcagca
     2401 gcacctattt catgacgcag cgtttttccg attgcaatcg agggactttc ttccaaaacc
     2461 ttttggttat tcctttgaag agaacaatcc cgctcaccca agtgaatcac atgtccatgc
     2521 tcatcaccta ggatttgaac ctcaatgtgc cgagctggat agataacccg ttctatgtac
     2581 atggcaccat tgccataatt ggccttggcc tcactagagg cagtttcaaa ggcagaaacg
     2641 aggtcatctg gtttttcaac cttacgaatc cctttaccac ctccacctgc tgaagccttg
     2701 agcataacag gatagccaat tttttcagca acaatcaaag cttcttcaga gttatgcact
     2761 tctccatctg aacctggtat aacaggcaca cctgctttaa tcatctgagc acgcgcattg
     2821 atcttatccc ccatcatatc cataacatga ccagatggac cgataaactt gatacctact
     2881 tcttcacaca tggtcgcaaa tttggaattt tcactgagaa atccaaaacc agggtgaata
     2941 gcttctgcct cagtcaagac tgcagctgat agaactgcat taatattgag ataagactct
     3001 gttgccttgc caggaccaat acaaactgct tcatctgcca aaagcgtatg aagagcttcc
     3061 ttatcagcag ttgaataaac cgctaccgtc gcaatcccca attcacgtgc cgcacggata
     3121 atacgaaccg caatttcacc acgattggca attaaaattt ttcgaaacat ggagaacctc
     3181 cttagttccc aattgcaaaa gtaaggatac cactggctgc aagcttgcca tccacttcag
     3241 cctttgcttc aaccacagct atggtgccac gacgttttac aaaagtcgct gtcataacca
     3301 attggtcgcc tggtacaact tgcttcttga acttaacctt gtccatacca gcgtaaaaga
     3361 ccagttttcc tttattttca ggttttgata actccaacac accagcagtt tgcgccaagg
     3421 cttccataat cagaacacct ggcataactg ggtattgagg aaagtggccg ttaaagaaag
     3481 gttcgttaat ggtcacattt ttgatagcaa caatggtatc ctcgctcact tccaagacac
     3541 ggtccactag aagcatagga taacggtggg gaagagcttc tttgattcct tgaatatcga
     3601 tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt tcttcgttag agacgagaat
     3661 ttccgttacc acaccatcct taggagctgg gatttcattc atgactttca tggcttcgat
     3721 aattaccaat gtttgacctt ttttgacact atcaccaact gtaacgaagg caggtttatc
     3781 tggtccagca gccaagtaaa ccactccaac aagtggactc tctacaagat ttccctcagt
     3841 agccacactt gcttcagctg gagctggaac ttcttctgct acagtctctg ctggagcaga
     3901 tgtaggagct actggactcg gtgttgctag aacgggtgct ggagcgactt gagttgcaac
     3961 ttcaggcaca ggtcttgctt cattcttgct aaactgcaac tcatccgtcc catttttata
     4021 agaaaattct ctcaaacttg actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa
     4081 attcatactt atctattctc ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa
     4141 ccaaaagtat ttgaaatagc gtatggaatt tctttctcca aaccttgtcc ataaacgaca
     4201 ttagcttcga tataatctga tacttcactt gtcccagctg tcattggtac aaagttatga
     4261 cgcatagctt cgatggtgac gatagcttct actgcacccg cagcccccag caaatgtcct
     4321 gtaaaagact tggttgatga tacaggtact tccttaccaa gaacagctac gatagcacca
     4381 ctttctcctt tttcattggc aggagttgac gttccgtgag cattgacata ggctacttgc
     4441 tctggagaaa tctcagcttc ttccaaggct agtttgatgg ccttgatagc tccctgacct
     4501 tctggatgtg gagaagtcat gtggtaggca tcacaagtat ttccgtaacc aaccacttca
     4561 gccaggatag tagctccacg tttttcagcg tgttcaagac tttctagaac caacatccct
     4621 gaaccttcac ccataacaaa cccattgcga tccttatcaa atgggatcga agcacgagtt
     4681 ggatcctctg tagtagagag agctgttaag gcttggaaac cagcgatggc aaaaggtgtg
     4741 atagaagctt ctgttcctcc caccaacatc acatcttgga aaccaaactt aatggagcgg
     4801 aaggcatccc caatcgcatc atttgatgaa gagcaggcag tattgataga tttacaaaca
     4861 ccgtttgcac caaaacgcat ggctacattc ccagaagcca tatttggtaa agcttttgga
     4921 agagtcattg gtttgacacg tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca
     4981 atttccttga ttccaccaat accagatgca acgataacac caaaacgatc cctattaaga
     5041 gcctctacat caagattggc atgatttaca gcctcttggg ctgcatacaa ggcatataaa
     5101 gaatagttat caaaacggtt ggtatctttt tttacaaagt atttatcgaa cggaaaatct
     5161 tggatttctg ccgcattatg cacatcaaag tcactatgat caaattttgt aatgccacca
     5221 atgccgattt tcccagttgc taaactattc caaaattctt ctggtgtatt tccgattgga
     5281 gatgttactc cataacctgt taccactact cgatttagtt tcattctttt cacctctagc
     5341 tttcgctaca tacttaagcc accatcaatg gcaatcactt gaccagttag ataatcttgg
     5401 cctgctaaaa atactgtcaa atctgcaacc tgctctgcct gcccaaattc tttcatcgga
     5461 atctgagcta gtgtagcttc cttaatctta tctgataaga tagctgtcat atcagactca
     5521 atcattcctg gagcaatcac attgactcgt atattccgac tagcgacctc gcgtgccaca
     5581 gacttggtaa agccaatcaa gccagcctta gaagcagcat agttagcttg accaatattc
     5641 cccatcaaac caacaacact agacatatta atgatagcac cttctctggc tttcatcatc
     5701 ggtttcaaga ctgattgtgt catattaaag gcaccagtca gattgacctt gagcactttt
     5761 tcaaaatctg cttctgtcat cttgagcata agagtatctt gggtaatccc tgcattgttg
     5821 accaaaacat ctactgaacc cagttctgca atagcttgat caatcatacg cttagcgtct
     5881 gcaaaatctg atacatctcc tgaaatggga accaccttga taccatagtt tgaaaactca
     5941 gagagcaatt cttctgagat tgccccacga ctgtttaaga caatgttggc tcctgcttga
     6001 gcaaacttgt gggcgatggc aagaccaatt ccacgactcg aacctgtaat aaagatattt
     6061 ttatgttcta gtttcatttt tttcctttca aaacttctac ttattttagt ctatttttct
     6121 aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta agtgagcagt ttgatcaatt
     6181 tttttaacaa aacctgacaa gactttcccc ggtccaatct cgataaagtt gcttatgcct
     6241 gcttcttgca tgaccccaat actttcatag aaacgaacgg gttccttgac ctgacgcgtc
     6301 aagagctgag caatgtcctc tttttgcatc acagcagctt ctgtattgcc gactagggga
     6361 caagtaaaat ctgaaaaact tacctgagct agagtttcag ctagtttctg gctagctggc
     6421 tcaaggagag cggtgtgaaa gggacctgac accttaagag gaatcaagcg tttggcacct
     6481 gcttcttgca aaagttcaac cgctcgatca actgcaacca cttctccagc aatgacgatt
     6541 tgtgcaggtg tgttatagtt ggctggagta accactccaa gttcagaagc tttttgacag
     6601 gcttcttcaa tgacctctac tggcgtattg agaactgcta ccatcttgcc agagtcagca
     6661 ggagtcgctt cttccatata ggctccacgc ttagctacca aggcaaccgc atcttcaaaa
     6721 tccaaggcgc cgcttgccac caaggcagag tattctccaa gagacaaacc agcaaccata
     6781 tcaggctgat agcccttttc ttgcaataaa cggtagatag caaccgaagt cgctagaatg
     6841 gctggttgcg tatagcgggt ctgattgagt ttgtcctctt ccgtatcgat gagataacgc
     6901 aaatcataac cgagcacctg actcgctcga tcaatcgttt ctttaacaat cggatactga
     6961 tcatagaaat cccgtcccat ccctagatac tgggcacctt gaccagcaaa taaaaaggct
     7021 gttttagtca tttcttacaa ctcctgtcca gcgagaggct tcttcttgaa ttttcttagc
     7081 ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt tcttctttag aaacaagccc
     7141 tgcgatttga cctgccatga cagagccacc atccacatca ccgtgaacaa ctgctttggc
     7201 tagggcacct gctcccattt gttcaaagat ttctaaatca ggatcttcct gcttaaaggc
     7261 atctttttca gccagttcaa aatctctagt caactgattt ttaatagcac gaacagcatg
     7321 accaaagtgc tgagctgaaa tcgtagtgtc aatatccctt gcttttaaaa ttttctcctt
     7381 gtagtttgga tgggcattcg actcttttgc aactacaaac cgtgtcccca cctgtacagc
     7441 ctctgcacct agcataaagc cagccgcagc accttcacca tccgcaattc ctcctgcagc
     7501 aataacagga atagatatag ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt
     7561 accgatatgc cccccagctt ccattccttc tgcaataaca gcgtctgcac cgattttttc
     7621 catgcgttta gctaaagcga cactaggaac aacaggaata acgattatcc cagcttcatg
     7681 gaaacgttcc atatacttgc ttggatttcc tgctcctgtt gtgacaactt taacaccttc
     7741 ttcaataacg agatccacga tatcttccac aaagggagat aagagcatga tgttgacccc
     7801 aaagggttta tcagtcaatg atttgatttt atcaatattg gccttgacaa cttctttcgg
     7861 ggcatttccc ccaccgataa ttcctaatcc tccagccttg gaaacagccc ctgccaaatc
     7921 accatcagca acccaggcca tccctccttg gaaaatagga tagtcaatct tcaataattc
     7981 tgtaatacgc gttttcatag tgcctccaac cttccttgct tacgtaatag ttcgatttca
     8041 ccataatttg acagtcaaac tattacctaa acaagaggga gtgggtttct ccctactcct
     8101 tctactaata ttctgcttat tttgcttgct cttcaacgta agcaaccaag tcaccaactg
     8161 ttttcaagtc attttctgct tcgatttgga tatcaaaagc atcttcgatt tctgagatta
     8221 cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa agttgattca agtgttactt
     8281 ctgatgcgtc ttttccaagt tcttcaacga taatttcttg tactttttca aatactgcca
     8341 tgataggact cctttaaaat aaatagtttt tttataacaa tgtgttcacc acatgattac
     8401 ctaaattgta agaatgagcg tgccccaggt caagcctcca ccaaagcctg atagaagaac
     8461 agtctggcta ccatctaaag ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg
     8521 gatactggct gcactggtat tgccatattc catcatattg gctggaagtt tggctcggtc
     8581 aacaccaatt tttctagcca tcttatccaa aatacggtca ttggcttgat gaagtagcag
     8641 ataatccaag tctgtcacct ctataggaga ttcatcaata gtctgcttga tagacttggc
     8701 tacatctcga atggcaaaat caaagactgt gcgtccatcc atcttcaaaa acgaatctgc
     8761 actttcttga tctgaaaatg gagaatgcaa acctgaatgc ccataagtta aacactcgct
     8821 gcgacttcca tcgctattga gactctcagc taagaaatgc tcttgctcgc tagcttctaa
     8881 caagacacca ccagcaccat ctccaaacaa cacagctgtt gatcgatccg accaatcgac
     8941 tgccttagag agggtttcac taccaatcac caagcctttt tgaaagcgac cagaagcgat
     9001 aaacttttca gcagttgaaa gagcaaatac aaatccactg caagccgcgg ttaagtcaaa
     9061 agcaaaggct ttattagcgc caatattagc ttgaacacga gcagctgtag agggcatcat
     9121 cgaatctgga gtaatggtag ctaggatgat aaaatccagt tcttctcctg ttattccagc
     9181 ttttgccatc agtttcttag caacctctgt agccaaatca ctggtagatt ctgttcttga
     9241 aatatgcctt tgtcgtattc ccgttcgact tgaaatccac tcatcattgg tatccataat
     9301 ctgagccaag tcgtgatttg taaccacttg ctctggcaca taatgagcaa cctgacttat
     9361 ttttgcaaaa gccattattt caaatcctcc aaaaattggt aaagattagt caagccttta
     9421 cccatgacag caatttcttc ctcgctcatg ccatcaataa ttttttctac catggccttg
     9481 tggaagcgtt tatgcagtct atgaatcaag tgacccttct ttgtcaaatg cagatgcacc
     9541 acacgacgat cctgttctga ccgaactcgc tcaatgtagc ccttacgctc taaattgttc
     9601 aaacttgtcg taacagttcc aagagttacc atcaactctt ttgacacttg acttggagtc
     9661 acatctggag ccttaccgat gacatcaatc gtatgcattt ctttgatgga gatatcctta
     9721 aaacgactac ctctcaagtt cacttcctca attacaagga cattgttaaa tatagatgtt
     9781 aaatattcat tgattcgttg gtagtccatt tttcatatcc ctccttcttc aaaaaacttt
     9841 tacaatcaaa acatttgaca gaaagaatta tatcaaaatt caaagaatga tgcaagtatt
     9901 ttttattttc ctgtaaattt aggtcttctt ctctccgaat gagctcgcac tccttctttg
     9961 aaatcctctg tttgagctaa ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc
    10021 cagtctttaa attggctctc ccaaaccaac ttcttaatgg cagcatagga attacttgaa
    10081 gcacgtctta attttttaag aagctgttct ctcgtctttt caagtttttc agcttcagaa
    10141 acgcggtaaa ccaaccccca ttctagggct ttttctgccg ttaaagcctc tcctgtcata
    10201 gctaattgag cagcacgcgt cacaccgata ctgcggctca agagatgaat tccccctgca
    10261 tctggagcca aaccaacacc aacaaaggct tggataaact tagccttatc cgtcgccaaa
    10321 cagaaatctg ccgcaacagc catattcgct gcggcacctg caacagcacc gtcaacttcc
    10381 attaaaacag gtttggctat ttgcttgatt ttataagaaa tcgtattgac caactctgcg
    10441 atttttgtca atgatggaat atcatcctca tccactgccc gcttcatctc taccaaatct
    10501 cccccaactg agaagacctt tccattggca ttgattaaga taaaatgcac agctggattt
    10561 tcttctgcca aagtcagagc ttctaaaatc tcctcacaca tgggaatatg aaaaccattt
    10621 gcgacctcag gacggttcaa ggtaaggatt gccaagtcct cttcaagctg ataaataatg
    10681 tgttccatca gggctccttt ttattttata agcagtttat actcttcgaa aatctcttca
    10741 aaccgcgtca gcttccatct gcaacctcaa aacagtattt tgagctgact tcgtctagct
    10801 tcttagtttg ctctttgatt tttattgagt gttaaattat ttcactgtca aagtatatct
    10861 tttttaggat cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaggc
    10921 tcgctttagg ctagaaagca tcttattttc ttcattgaaa aaagcctttc tttctgctat
    10981 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    11041 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    11101 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    11161 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    11221 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    11281 tagaaaaaat ttctaaaagc attcacgcct tggccactct tcctattgaa gaaaatgaat
    11341 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    11401 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    11461 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    11521 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    11581 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    11641 tcaaagctga cctctatgaa aactttacgg acgttgatgg tatctttgca gctcaccctg
    11701 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    11761 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    11821 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    11881 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagca
    11941 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    12001 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    12061 tcattctccg ttctcgccaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    12121 ttcaaaaggc taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    12181 ttggtgaaaa aatgaagagc catatcggag tgactgctac tgcgacacgc gctctatctg
    12241 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct attatgtttg
    12301 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggag
    12361 aaagtaagga agactaagca tggctcaatc acttaacaaa acagtactcc tcagtacgac
    12421 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    12481 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    12541 caatcagatt ggagccaatg tcactggatg caaaatcagc cgccacttcg aagtctttac
    12601 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    12661 cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    12721 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    12781 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    12841 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    12901 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    12961 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    13021 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    13081 taacatcttg gtcaaggttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    13141 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    13201 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    13261 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    13321 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    13381 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    13441 aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    13501 ctttatgttg gatgaacatg gaaaagaagg ctatactgaa gtcatcacac cttacatagt
    13561 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    13621 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    13681 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcta tgagtccgtc
    13741 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    13801 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    13861 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    13921 tgctctctct actggagata tgggcttctc agctgcgaag acttacgact tggaagtgtg
    13981 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    14041 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    14101 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    14161 ccaaaatgaa gatggttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    14221 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    14281 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    14341 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagtcatc ttgagcgagg
    14401 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    14461 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    14521 caatcaagac ccaagtcggc aggctggttc ctcctactcg aactagagaa gccaagagta
    14581 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    14641 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    14701 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    14761 tccccacata gagaaccgtc cagcttggag tggcatgaac cctccgccct ggacaagcaa
    14821 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    14881 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    14941 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    15001 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    15061 gactaaaaag atgggataga accggcaacg tatctaaaat aagatttcca gctcctgcca
    15121 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    15181 atgttagttt cagtataaca taaaagcgct taaatgagga tttaaaaaaa cgaagtccgc
    15241 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    15301 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccaaaa
    15361 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    15421 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    15481 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    15541 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    15601 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    15661 atagatgaga caatttggta ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    15721 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    15781 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    15841 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    15901 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    15961 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    16021 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgactgg
    16081 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    16141 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    16201 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    16261 atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca aggaagatgg tcaagagggc
    16321 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    16381 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    16441 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    16501 ggcagctgca ataggagcag aacccagacc agattcgttt gagaacacac cacgcgccac
    16561 accattttga atagccatcc gaacgctagc accagcaaat ccacctaccg cagcaagggg
    16621 actaaaagct gaggtaaaga ctaaagcgat tgtgccaggg atttttccaa tattaaagaa
    16681 aataactgta agagttccta agatataaat gatggccata aaaggaacaa cagtagttga
    16741 aaccttagaa atagacttga gtccaccaaa gactgcaatc gctacaaaga cagacaagac
    16801 gagagctgtg atggctggcg aaatcgtcgt tgtattttgg atagattctg caatcgagtt
    16861 gacttgggtg aaggttccga ttcccaagag agcaaccaat actcctgcta ctgcaaacaa
    16921 aacagcaagt ggtcgccact tttctcccat ccctagaagg atataatgca tgggacctcc
    16981 cgctactgca ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc cttccgcata
    17041 cttggtagcc attccaaaga aagccgccat ccacatccaa aatagagctc ctggtccacc
    17101 aaccttgata gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg atgccaaggc
    17161 tgtacacaga gctgcaaaac tggatacatc accatgtccc ttatcctgga taaaaataag
    17221 ctgaaaagcc ttgggcagac gcaaaacctg caagagtcct agccgaatag ttaggtaaat
    17281 ccctgttccg accaataaaa tcaagagggg cggtccccaa gcaaaagcat cgattgattt
    17341 aagcaattct aacatttcct tctcctatcg tttcaacccc aaaagaaaga gcacatgcaa
    17401 gatacatgta ctctggaatg cttagataaa tgctaaaaag cggtctatcc tagctctgtc
    17461 cttttacctg agagtttgag cagttgcctg ccttgcccct tcggtgcctt tacggtctct
    17521 ccagagttcc gtccatttac agtcatggaa aatcaaacga ttccccactt ctattaaact
    17581 tcattcggtg ttggtattta attgattcta attttacaaa aaatgttggc ttttgtcaat
    17641 gtgtttatta gtaaaaatta gttcaacagt ttttacttta taaagtccag aatactgcta
    17701 tcctttaaaa gtgacaatag tcgcaccact gcctccagca ttttgtgggg catagccgaa
    17761 actcttgaca tgtttgtttc tttgcaagta tttggtaact ccttcacgga tgactcctgt
    17821 tccgatacca tggatgatat caacttgagc catattgtta agcaaggctt ggtcgatgaa
    17881 gatatctagc tcattcatgg cttcttcata gcgcttgcct cgaagatcca gtctagcttg
    17941 aggtcctcgc ccagaagttc gtttcacaac attgacctgc ttcttcttga ctggtttttc
    18001 ttgctgggct tgaacaagat caaactcttt ctcttccaag gtcatcttaa tcaagccaac
    18061 ttgggcttcc cagcggccgt ccttgagttg actggtcaag gtaccacgct gaccataact
    18121 gagaaccacg atatcatctc ccacctttgg agctcgtttt ttcttggcct tttgaaggac
    18181 cttgttttta gacaagtcca ctttttcagg agccaatttt ttcaacttgg ccttggcttc
    18241 aatgatttcg tggggcttga gttgggattt actgtggaga tttttgagaa tctggtcact
    18301 ttcacttagg gccatatcca caatctcagc agcctgttca cgcgccttgt taagctcggt
    18361 ttccttttca cgattaagct cgttgtagag tttttttagc gcacggttca tcttgagatt
    18421 ttcttgctcc acctcacgga tattgtccaa gcgtttgcgg ctttccagcg tctgctcttc
    18481 taattgctca atgatacgat tgacgtcatt gtcctgatcg atctgctgac tggcatctcc
    18541 tacgataact tcagataggc ctagacgttt ggcaatttca aaggcattac ttcggccagg
    18601 aacaccctgc ataaagcgat aggtcgggcg aagagttgca gtatcaaact ccatactggc
    18661 attttgcaca aaggctgtct caataccgta ggccttgagt tctggatagt gggtcgtcgc
    18721 catggtcttg atttgacgca ggcgaaggtc ctccagaata gccatggcaa gggctgctcc
    18781 ctcttgggga tcagtaccag cccccaactc atccaaaagt aagagtgaat gttggttgac
    18841 cttgccaaga atatccacga tattggtcat atggctagag aaggtagaca agctctgctc
    18901 aatagactgc tcatctccaa tatcagcaaa gatttcttca aaaataccaa cacgacttcc
    18961 cctgtctgct aaaatcggca atcctgactg ggccatgacc tgtgtcaagc ccagagtttt
    19021 gagcatgatg gtcttcccac ctgtattggg acctgtaatg acaatagctg ttaaatcttg
    19081 accaaaatgg acatcatttg cgacggcatt tttgaccaaa ggatggcaga catggagcag
    19141 ttgaatctct tgattttctg acagctgagg cacgactgct tgtctttctt ggataaatcg
    19201 aaccttggca cgaatcaagt ccagatgacc gataatccaa gcgtcattag caatctcagt
    19261 cgcatgaggg cggacacgct cagaaatttc ttggagaatg cgaagcattt catagcgctc
    19321 atctgctcgc agactagcaa tttcttcgct cagtttgact acctcacgtg gttcgatata
    19381 gacggtgttt ccactagcag aaatatcatg aacgacacct gcaatcttat tgcggtaggt
    19441 gtttttgact ggtaaaacct gacggccatt tctgctagca acaattcctt ctgtcaacat
    19501 ctgcgctttt tgcttgagca agtcttgtaa aacatcgcgt acctgactct cgctatcatg
    19561 tatttttcga cggattcgcg ccaattcttc actggcaaaa ttttcaatga aacccgcatc
    19621 attaaaggcc tgaagatttc cttgtaattg cggaaaatca tgtaatttct caaaccaaag
    19681 ggctaattct tccaagctga cattttccag attggcgtaa aaactttgaa gttctcggct
    19741 ggcaagaagc acgcgtttca agagtaggaa ctcctcgata ttgagatccg ctcccatctc
    19801 caacctcttg cagactcctg caatttcctt agttgagaga atagtaaaat gcggttgctc
    19861 gacgaaaaga gcctgcattt ccttcatctc agcaaaagcc tgtttgattt tatctgcttt
    19921 ggcagtcgga gccagttgtc tcaattgctc caagccctgc tcggtcaaca aatgaggctc
    19981 aaacaaggcc ttgaccttat cgaactctaa tgtttctaat attttcttat tcatctttat
    20041 ttccttgtct ttgaatgtct aggtgtggta gctttttaca ttctgataga ttcgagtcaa
    20101 atctgtaaac aaagggctag gaaaactcct gcccttttta tccgattaaa tttgtcaccc
    20161 agatttgttt gagccaactg gttgttatcg gtatgctctg gatgatgtgt tttgcgacga
    20221 tactcttttc aagaggattt tgtataactg ccatggggat ggtcgccaag attgtcaagg
    20281 ccatttgcaa gacaaataag gtcaccaaca tggacaagat acctgctgaa acttggaaca
    20341 acttaccacc cagttttttg ctaggaatca agtgtaagag aagaccaagc aaacgaccaa
    20401 tgctatagac aatcccaaat acaagcaagt agccgatgcc tgcataaaag accttatcca
    20461 gctgaaagag ttgatccgat gggaaaaaga aagtcccctg accttcctgc gaatttgcat
    20521 aagggagcaa taaatggaat tgctctccaa gccccttata aaactggcca gccataaaag
    20581 ccgatgccat ggctgaaatc aggtaataaa cctgtaagag caggcctctc cgatagccga
    20641 tataaaatcc ccaaaccaag accaatagaa gaaggaatga aatcataagg aatcctcaat
    20701 cttgctctgt tcttgcttac aagtcacaag cttgtgacgg agttcttcta gctcttgctc
    20761 cttatcgtca aattcaatct cacggctgag ctgagttgat aaacagttga ctgccaacaa
    20821 aagagcgatt gtttcatcat ctgcgctagg catttgttct ttaattgctt ggtatttttc
    20881 tgtcgcaacc ttagcgattt cctccataaa aaggttatca tgttcgcttg tcaaggttaa
    20941 cgattttttc ccgaatgtaa atttgaatcg atttagattt gccataaaat tcacctcacg
    21001 atattatacc aaaattcgct aattttgtca gtttttacaa attttactgc ttttgtggta
    21061 caatagaaac tatggcaagt atcacactca caccaagcga aaaggatatt caggcttttc
    21121 ttgaacacta tcaaaccagt ctggctccta gcaagaatcc ctatatccgc tactttttga
    21181 aactacctca agcaacggtt tctatctata cttctggaaa aatcttgctt cagggtgaag
    21241 gggctgaaaa atacgccagt ttctttggct atcaagctgt agagcaaacc agcggacaaa
    21301 atcttccttt aattgggaca gatgaggtgg gaaatggttc ctactttggt gggcttgcag
    21361 ttgtggctgc ctttgtcaca cctgaccagc acgacttttt acgaaaactc ggtgtggggg
    21421 attctaagac tctgaccgac caaaagatcc gtcagattgc tcctattctc aaggaaaaaa
    21481 tccagcacca ggcactcctt ctctcaccca gcaagtacaa cgaggtcatc ggaaaccgct
    21541 acaatgctgt ttcggttaag gttgccctcc ataatcaggc tatctatctc ctccttcaaa
    21601 aaggtgttca gcctgagaaa attgtgattg atgcctttac cagtgctaaa aattatgaca
    21661 agtacttggc acaagagacc aatcgtttca gcaatcctat cagcttagaa gaaaaggctg
    21721 agggcaaata cttggctgtc gcagtttctt ctgtcattgc gcgtgatctc tttctggaaa
    21781 atcttgaaaa tttgggacga gaactgggtt atcagcttcc aagtggagct ggaacggctt
    21841 ctgacaaggt ggctagccag attttgcaag cctatggtat gcagggactc aacttctgcg
    21901 ccaaattgca ctttaaaaac actgaaaaag cgaaaaaacg cttagaaagg taagttatga
    21961 attcatttaa aaatttcttg aaagagtggg gactgttcct cctaattctg tcattactag
    22021 ctttaagtcg tatctttttt tggagcaatg ttcgcgtaga aggacattcc atggatccga
    22081 ccctagcgga tggcgaaatt ctcttcgttg taaaacacct tcctattgac cgttttgata
    22141 tcgtggtggc ccatgaggaa gatggcaata aggacatcgt caagcgcgtg attggaatgc
    22201 ctggcgacac cattcgttac gaaaatgata aactctacat caatgacaaa gaaacggacg
    22261 agccttatct agcagactat atcaaacgct tcaaggatga caaactccaa agcacttact
    22321 caggcaaggg ctttgaagga aataaaggaa ctttctttag aagtatcgct caaaaagccc
    22381 aagccttcac agttgatgtc aactacaaca ccaactttag ctttactgtt ccagaaggag
    22441 aataccttct cctcggagat gaccgcttgg tttcgagcga cagccgccac gtaggtacct
    22501 tcaaagcaaa agatatcaca ggggaagcta aattccgctt ctggccaatc acccgtatcg
    22561 gaacatttta agagacctaa gaggccgaga atcaccaatc tcagcctctt cttctatcgt
    22621 gagaaaatga ttggtaacta tctaaactta ccagaacaga aacacctcaa ctctcaccta
    22681 ttcatgcaaa ggaattttat ggaagtttat ttttcaggaa ctattgaacg gattattttt
    22741 gaaaatccca gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag
    22801 gattttgatg attttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa
    22861 gactatactt tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc
    22921 agtcgttatg atcgcgcaaa accaactagt aagggcttgg tcaagtactt ttcaagtagc
    22981 catttcaagg ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat
    23041 accattgacg aaattttgca acacccagaa aagttagaag gcatcgtagg actctctgcc
    23101 aaaaatcgcg aggctttcgt ctccactctc cgtctcaact acggaacgga gatgattttg
    23161 gccaaactag ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac
    23221 aaggaagaaa cccttgatgt ggttgaaaat tatccctacc agctggttga ggatatcaag
    23281 ggtttgggct ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct
    23341 cctgaacgct tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacaggg
    23401 gacacctatg ttgaagtacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc
    23461 cgtcccgtgg aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa
    23521 gacaaggttc agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa
    23581 ggcatccgca gtcacttggt tcgtatcctt gaaaaaggaa aacagaagag tcatgattta
    23641 gaaaccattc aaaaacatat cactactatc gaggaagagc tgggaattga gtatgataac
    23701 attcaaaaac aggctatctg tgatgctatc cagaataagg tctttatcct gacaggtgga
    23761 cctggtactg gtaagacaac tgttatcaat gggatcatcg ctgtttacgc cctgttagag
    23821 ggacttgacc tcaggaagaa aagcaacctg cccattcttc ttgctgctcc aactggacga
    23881 gccgctcgtc gcatgaatga attgacaggc ttgcctagcg cgaccataca ccgccacttg
    23941 ggaatgacag gtgacgatga taccagtcat ctggaagatt acctagatac tgactttatc
    24001 atcgtggatg aattctctat ggtggatact tggctggcca atcaactctt ctccaatatc
    24061 tcttctaaca gtaagatcct catcgtaggt gacagtgatc agctaccttc tgtcagtcct
    24121 ggacaggttc tagcggatct gcttcatatt cctttgattc ctcagactcg cttggaaaaa
    24181 atttatcgac aaagcgaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc
    24241 atcttgccag ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt
    24301 ggccatattc ctgccaccat tgaaaaaatc ttaggtgccg ccctcagaaa tggtattcct
    24361 gcccgtgata tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc
    24421 aatcagctca tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct
    24481 cctcagtgcc actatcgtaa gggagacaag gtcattcatt tggttaacga tactgaaatc
    24541 aatgtcttta atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa
    24601 tcgaaacaag acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac
    24661 gaatggtaca agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag
    24721 ttcccagttg tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc
    24781 atctacacag ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc
    24841 ttcgaccatg ctacccaaca catcggaact gcccgaaaaa cctatctggt tgaacgcttc
    24901 agtgatctac tgaagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca
    24961 agtgcctctg aacaatccta catcctaacc gaagaaaact gggaccgcat cccagccatg
    25021 attgggatta cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaccc
    25081 acctgaattg gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtaatc
    25141 aattcaacag cttttttgat tgtgatatca tgtttcaaca tgtcagctga aagcaagttt
    25201 tgaacttgtg caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt
    25261 tcttcttctg aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc
    25321 ttagtacgtg actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca
    25381 gtaatttgga agtacatgtc agggttgatc ccttgacgtt gcaagttccc aaggaattca
    25441 tttactgaac ggtgaacttc ttcgtggatc atttcttctg gaagttctac gatttcagca
    25501 ttttctacag ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta
    25561 gcagcagcca attctttgcg gtatttttct ttcaagtcag caagtgtttc aacttcttca
    25621 tcaatgtctt ttgcaagttc atcgtcaaga gctggaactt ctttagcttt tacttcgtgg
    25681 atagttgtca cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg
    25741 aatgttacga taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa
    25801 ccagggatga attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca
    25861 aattcaacac cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gtcttcagca
    25921 gcagcttcct tgataaccaa ttcagccagg ttgttgcgtt cgcgttcgat acgctcttcg
    25981 acatcagcgt cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca
    26041 cccaatttta cttcaggttt tgtaacaact tcagcagcga taacccagtc ttgacctttt
    26101 tccattgaag ttacgtcaat ttttggttgg gcaaccactt caagaccagc ttcttttaca
    26161 gctgcttcat aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca
    26221 ccaaattttt tatcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta
    26281 agagatttct tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag
    26341 atagtgaaag tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg
    26401 tcatttctcc ttaaaatttt ttaaatacag tctattataa cattttctat aaataaaatc
    26461 tagactaatg cagacaatta tagacaacaa gcctttttcc taactaaaac actcataaag
    26521 tatagacaat tttagactac tttggatatc ttttggttag tcattttcca aaactaattt
    26581 atctaatttt taagttatat ttg
//
LOCUS       DAVHIU010000017        26598 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVHIU010000017 DAVHIU010000000
VERSION     DAVHIU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.017"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(207..425)
                     /locus_tag="VVN06_000592"
     CDS             complement(207..425)
                     /locus_tag="VVN06_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU5562192.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            583..729
                     /locus_tag="VVN06_000593"
     CDS             583..729
                     /locus_tag="VVN06_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562193.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            complement(1321..2010)
                     /gene="rplA"
                     /locus_tag="VVN06_000594"
     CDS             complement(1321..2010)
                     /gene="rplA"
                     /locus_tag="VVN06_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5562194.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2218..2643)
                     /gene="rplK"
                     /locus_tag="VVN06_000595"
     CDS             complement(2218..2643)
                     /gene="rplK"
                     /locus_tag="VVN06_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5562195.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(2766..3482)
                     /locus_tag="VVN06_000596"
     CDS             complement(2766..3482)
                     /locus_tag="VVN06_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU5562196.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3500..3961)
                     /locus_tag="VVN06_000597"
     CDS             complement(3500..3961)
                     /locus_tag="VVN06_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5562197.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(3963..5000)
                     /locus_tag="VVN06_000598"
     CDS             complement(3963..5000)
                     /locus_tag="VVN06_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU5562198.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(5098..6423)
                     /gene="brnQ"
                     /locus_tag="VVN06_000599"
     CDS             complement(5098..6423)
                     /gene="brnQ"
                     /locus_tag="VVN06_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU5562199.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6594..6686)
                     /locus_tag="VVN06_000600"
     CDS             complement(6594..6686)
                     /locus_tag="VVN06_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5562200.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6729..6881)
                     /locus_tag="VVN06_000601"
     CDS             complement(6729..6881)
                     /locus_tag="VVN06_000601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5562201.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(7041..7619)
                     /locus_tag="VVN06_000602"
     CDS             complement(7041..7619)
                     /locus_tag="VVN06_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU5562202.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7673..9073)
                     /gene="pepV"
                     /locus_tag="VVN06_000603"
     CDS             complement(7673..9073)
                     /gene="pepV"
                     /locus_tag="VVN06_000603"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU5562203.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9086..9691)
                     /locus_tag="VVN06_000604"
     CDS             complement(9086..9691)
                     /locus_tag="VVN06_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5562204.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            9862..10662
                     /locus_tag="VVN06_000605"
     CDS             9862..10662
                     /locus_tag="VVN06_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562205.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10846..11679)
                     /locus_tag="VVN06_000606"
                     /pseudo
     CDS             complement(10846..11679)
                     /locus_tag="VVN06_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11669..13516)
                     /gene="uvrC"
                     /locus_tag="VVN06_000607"
     CDS             complement(11669..13516)
                     /gene="uvrC"
                     /locus_tag="VVN06_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5562206.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(13556..14257)
                     /locus_tag="VVN06_000608"
     CDS             complement(13556..14257)
                     /locus_tag="VVN06_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU5562207.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14336..15568)
                     /gene="murN"
                     /locus_tag="VVN06_000609"
     CDS             complement(14336..15568)
                     /gene="murN"
                     /locus_tag="VVN06_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU5562208.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15573..16793)
                     /gene="murM"
                     /locus_tag="VVN06_000610"
     CDS             complement(15573..16793)
                     /gene="murM"
                     /locus_tag="VVN06_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU5562209.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16905..17684)
                     /locus_tag="VVN06_000611"
     CDS             complement(16905..17684)
                     /locus_tag="VVN06_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU5562210.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17753..19414)
                     /locus_tag="VVN06_000612"
     CDS             complement(17753..19414)
                     /locus_tag="VVN06_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5562211.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19589..21811)
                     /gene="recJ"
                     /locus_tag="VVN06_000613"
     CDS             complement(19589..21811)
                     /gene="recJ"
                     /locus_tag="VVN06_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU5562212.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22088..22846
                     /locus_tag="VVN06_000614"
     CDS             22088..22846
                     /locus_tag="VVN06_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5562213.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22886..23650
                     /locus_tag="VVN06_000615"
     CDS             22886..23650
                     /locus_tag="VVN06_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5562214.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNSKTIGVAQGSITQCLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23663..24340
                     /locus_tag="VVN06_000616"
     CDS             23663..24340
                     /locus_tag="VVN06_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5562215.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGFFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24350..25009
                     /locus_tag="VVN06_000617"
     CDS             24350..25009
                     /locus_tag="VVN06_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5562216.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVHNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYKAGMSQGFTSAQILYYIILPQASRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25304..26494
                     /locus_tag="VVN06_000618"
     CDS             25304..26494
                     /locus_tag="VVN06_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU5562217.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLCRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         7584 a         6139 c         4857 g         8018 t
ORIGIN      
        1 ttattagcaa ggcttctact gattataacc taattcttag actacttcaa gactaactgt
       61 ccaataataa tctacagata aatcatccac tcttaatcaa attttattca atccatctac
      121 tcattcccaa aaagttgctg tataatgcaa gacatttttc ttactgcttt tacttttcct
      181 taccctcaat ttagtgagct aataaattat tcttcttcaa aaggttgaga tttatctagt
      241 aactctaaaa cattaatggt aggtagcaac attgcttcac tgctatcaat tgccgaaact
      301 attgaaacaa tagaacgtaa ataaggataa agaatagcag taccatttac gtatagaata
      361 tcggtgatat tttcatctat atttatttca aaatatccta ttacagttac agatattttt
      421 ttcatggcat ctttgtcaac taaagaaaca ctaagtttta cggctccatg gctataatca
      481 tctgtatgac caaccttaac tatgggatat aatatatcga aggtaaatgt gaacaaactg
      541 attaggaaat ttaaaccaac ttctaacaat gtgttatact caatgaaaac caaagagcaa
      601 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtgga tagaactgac
      661 gaagtcagta accatacgca cggcaaggcg acgttgacgt ggtttgaaga gattttcaaa
      721 gagtattaga aagctcgatg gactattcca gattcattcc actagatttc gaaatcgagt
      781 aggggataat ctctagcccc tctcacacca ccgtacgtgc cgttcggcat acggcggttc
      841 aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc agtccacgtt
      901 tttcaaggac tggttttgat atagcacgtt taagtaccga cttctgagct actatagtag
      961 attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg atttgactgt
     1021 cctgaacgat tcgtcctatt cttatttcat tttattataa ttgatagtgg tcgccccagc
     1081 cagatacctt atccgctata taggccttat tatcacgttc ctgttcgtag agccacgatt
     1141 tcgctatccc ttcctctgac gattcttatt tccagcgtca gacttgggaa tcgctattgg
     1201 gttcaccggt agtaggtgcc caccgaggac ttacacctca gatatacgac atgcccgtcg
     1261 tacatcaaaa aaagttgaga catagtgtct tcaacttttt tacattactt taactactga
     1321 ttaaagtgag tttacgtcaa ctttgatacc aacaccttga gtagttgtga tagtcaagtt
     1381 tgttacgtaa gttcctttag ctgtagctgg ttttactttt tggattgttt cgttgaaagc
     1441 tttaaagttt tcaaccaatt tttcagcttc aaatgatact ttaccgatga ttgcttgaac
     1501 gttacctgca cggtcagcac ggtaagtgat tttaccacct ttagactctt caactgcttt
     1561 agcaacatcc attgttacag taccagtttt agggtttggc atcaagttac gtggtccaag
     1621 gacacgtcca agacgtccaa caagagccat catatcaggt gtagcgataa ctacgtcgaa
     1681 gtccaaccaa ccgtcgttga ttttagcaac aaggtcatct tcaccaacaa agtctgcacc
     1741 agcagctttt gcttcttcag cttttgcacc acgtgcgaaa acaagaacac gtgaagtttt
     1801 accagtaccg tttggcaata ccattgctcc acggatttgt tggtcagctt ttttaacgtc
     1861 gatgttcaag ttgtaagcaa cttctacagt tgcatcaaat tttgcaaagt tagtttcttt
     1921 tgcaagtgct acagcttctt ctacactgta tgcttttgtg ctgtcgattt tctcaagagc
     1981 agcacgaagt tgtttgcttt ttttagccat tttctattct ccttgtaagt ggttcaatcg
     2041 attttcatct cccacgtcac ttctcgaaat gatgaagtct tgcgggtttt cagtctacta
     2101 tttttctctt cctacggcct taagctccct tgctgtatcc aagtacaagc ctcagtcact
     2161 tgcctagtat gaaaagcaac taatagactg ctgtgagatt ttctactgtg ttattgatta
     2221 gtcaacaaca gtgaatccca tagaacgagc agtaccttcg atcatacgca ttgcagactc
     2281 tacgtttgct gcgttcaaat ctggcatctt agtttctgca atttcttgta cttgtgcacg
     2341 agtaactgta gcaactttag ttttattagg tgtacctgat cctttttcaa cacctgcagc
     2401 ttttttcaaa agaacagcag ctggtggtgt tttcgtaacg aaagtaaatg atttatcttc
     2461 gtaaactgag ataacaactg gaatgatcat accagcttgg tcagctgtac gagcgttgaa
     2521 ctcttttgtg aatcccatga tgttgatacc agcttgacca agagcaggtc caaccggtgg
     2581 agctggtgta gctttaccag cagggatttg caattttaca agtttttcga cttttttagc
     2641 catttttaaa tcctcctttg tggttttggc ggtaattaga gatttttacc tcccacaagt
     2701 atgctttaca catacctttc cattatacac tatttatcta aaaatgcaag agaaaacttt
     2761 catttttaat cgacataatc gccgccttca aagccatagt actcttccaa actgagacca
     2821 cttgcagcaa tttctttggc ttcttttcca acgtagcgga gatgccattc ttctgccatg
     2881 tagccagtct ccttttcctt gcctttaaga tagcgaacaa caaagccata gtcagccgca
     2941 tggtccaaga gccactgggc tgccttttct tcagtaacta aatccccatc tgtcccaatc
     3001 aaatcaaaag caagacccgt ctggtgttca ctgtaacctg ggcgtgctga gtagcgatct
     3061 gctgcttcct taccatcttg attgacatag tcttggtaga gcttagcctg agtttcataa
     3121 ctcctaaacc cactgtattg gtcactgatt ggatagccct cagcctgcat agctgcgatt
     3181 agtttgagca actctgcctt ggctgttgga ttttctcctg gattgtaatc ttttgacaaa
     3241 ggataacgtt tattagctag gataatctca tcgtatttcc cttggatact ataatagctc
     3301 cctttattgc taacttctgc ctttttctga ctagaagctt gagatttgct acctacagtt
     3361 ccttccggtt gactgctttg ctccgttttt tgagtgtttt cttcaacttt ggttttttct
     3421 tgcgaacaag ctgcaagggt caatgctagt aaaccagata aaatgacata tctttttttc
     3481 atttctttct tcctctctct taggccaaat acttctccaa ttcatgagat aagattttaa
     3541 tcatctcttc cagctctggc gattgtactt ggcaatcttc tgccgccatt tcttcccagg
     3601 gcacccacca gactgggcca cggccattca aaccgtgact cttcatatca cctgctcttc
     3661 ggggaaaaag atgccaatga gcgtgggcat cgccatttcc taggagttca atattcatct
     3721 tttcagcctc aaatgctttg gcgactgctt cttggactaa actcatttcc tctaaaaagc
     3781 gaagttttac agaagtttcc atatgatgga gttctgtgac atgatctttt gctaaaaata
     3841 aggtatagcc cttaaagtat tggtagtcac caataacaac atagcctgtt tctagttctt
     3901 ttacaaagta gggattttgc ccagctttaa tgagttcgat tctctgacag attaagcaca
     3961 ttctatgata ccgcctcgct cttaagataa gcatcaacca tacgctcagt cgctattact
     4021 gaatcgatgt gggtgcgctc ataagagtga ctggactcaa taccagctcc aagaagtgca
     4081 tgcttgacct ctgcacctgc tgacatcgct gctgaagcgt ccgatccgta aaatggatag
     4141 atgtcgagct taaatggaat atcttgctct ttagccaagg caaccaagtg ttgacggaag
     4201 tcatagtgat aaggacctga agcatccttg acacagatag atactgtgta ctcatctgtc
     4261 tgctggtcat ctcccatagc tcccatatcc acagccagat attctactac ctgagcagga
     4321 atgttagaat tagcaccatg tcctacttct tcaaaaactg aaaaagcaaa atgagttgtt
     4381 actggcaatt caatcttctc tttcttataa atacgaagca ggttgagcaa aatcgctgcg
     4441 ctgaccttat catccaaatg acgagacttg ataaaacctg tatcagtcac aacagttcgt
     4501 ggatcgaagc tgataaaatc accgacctca atcccaagag cacgagtttc tttttcattg
     4561 cttactttta catccaaacg cacttccata ttgtcctgcg tgcgttctgc agttcctgca
     4621 tccttgtaga catggcaaga agtttggtgg atgaggatgg ttcctgatac cttttgacct
     4681 gtgctagcca catgaacggt acagttttca ccttcaatca tgttccaagg aaaaccaccg
     4741 atacggtcca atttaagacg accatctggt ttgacagcac gaacaatagc acccagcgta
     4801 tccacatggg cagtcacata gcgatgttgc tcatcatttt gacctttgat ggtcacattg
     4861 acaccaccct tagctgtgcg aactggctgg taaccaaaac cttctagagt cttgactaaa
     4921 taattcgaaa tttcacgagt gaagcccgtt ggcgatgcga tggctgtcag ttctttgata
     4981 tattctactg tttgattcat ttcttcacct ctttcgctac ctattataac acatttatca
     5041 tcggataaaa aaagaaaatc actcctgtag acttggctac aaaagtgatt taagtgatta
     5101 ttccatttca aaaacatcgc tttcttgctt gtttggtaga accaatgaga gcaagattcc
     5161 aacaatggct ggcaccaacc atgggagaga tgccttggca aaaggaagaa cgctaacaag
     5221 atttgcaaga aactcaacct taaacgagct tcctagtacg cttgcaatgg caataactgt
     5281 aaccacagca attgtcaact gcatacctgg ttttgaaagg gcaacaaatt tgttgacaat
     5341 gacaatcata acgatagcaa tcgtgattgg gtacaagata accagtactg gaattgagta
     5401 cttgataatc gcatcaagac ccaaattggc aatagcaaat ccaatcaagg taaaggctgt
     5461 cgcataaacc ttgtagctga tttgtgggaa gcgctcatta aagaactcag ctgttgacac
     5521 aatcaaacca acagtcgttg tgaagcaggt tacggtaacc atagctgcaa ggaagagttg
     5581 agctgttgag ccaaagattt cttgagtggc ttgtgacaag atgtaaacac ctggtgttcc
     5641 acccttcatc gcttcagctg gtactgggaa atgatttcca agaaaaccta aaccgatgta
     5701 aagagcgctg aaggcaaggg caacaacgat accaacaacc caaatagttg aaatgtattc
     5761 tttcttactt gaaaatccaa gttgtttcaa ggtttgaact gcgattacgc taaaggccac
     5821 tgaagcaagg gcgtccaagg tattgtaacc ttctaggaaa cctgtaccaa aggcagaagc
     5881 ttgataagca gctgaagcag cttgaggact tgttccacca tatttgatag ctcccagaac
     5941 gaccaagata acaatcaaaa ttgcaaagac tggcgttaaa acacgtccaa tgcggtctaa
     6001 gatttttgat ggattaagcg aaatcaaata ggctgccgca aaatacagaa ccgtaaatac
     6061 aatcaagcca agtcctttat ttgcatccga caaaaggggg ctaatcccta cttcgtaagc
     6121 tgttgtagca gtacgtgggg tagcaaagaa tggaccgatt gacaagtaaa gaactgagag
     6181 gtaaagagtc gcaaaccaag gcgctatctt cgttgaaatc tcgtagatat atcctttagg
     6241 atttagcgtt ccaataataa gggtcaagac ggcgatacca acgcctgaaa agacaaaacc
     6301 tgcgatggca ggaagaaaat gttctccaga tagagcacct agagaaggcg gaaaaatcaa
     6361 gttccccgca ccaaaaaata ttccaaacag gagcaaacct gttagggtac cttttttagc
     6421 cataaaaatc tccttcatat ttataaaata ctgacctagt ataccacgat taggagtctg
     6481 aaaaaagcat cacgaggcaa atattaaatg ttttcaattt tcttaaaaat aagaattatc
     6541 taaaaataaa actctcctac ccagtccaca ccaagtagga gaaatgcaaa attttaaagt
     6601 tcttcaaagg ctaacatgcc accctggaca ttgataacat tataaccttg ttctaacagg
     6661 aattggcaag cacgcgccga tctcattcca gatttgcaaa taatataatg caagcgatct
     6721 ttgtccaatt aatcataact atcagccaat tgactaagcg gtaggttgtg ggcacattct
     6781 aaatgaagag ctgcaaactc atccacttct ctcacatcca ctagagaaag ctggtcgttt
     6841 tggtaaagct ggtaaaatgc gtcaaaggct atttctttca ttctttttct tctaatactc
     6901 ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tgcgtatgtt accgacttcg
     6961 tcagttttat ctgcaacctc aaagctgtac tttgagcagc ctacgactag cttcctagtt
     7021 tgctctttga ttttcattga ctataaaatg gttttaattc tttttttcaa atctggcact
     7081 acttctgcct caaaccaagg atttttggcc atccagattt gatttcgtgg tgatgggtga
     7141 actagcggaa aataggctgg cagatagtct ttatagtgtt tcaccctctc cgttaccttc
     7201 ccactgattt tctcctgtaa atagtaggct tgggcatatt gcccaatcaa gagggttaac
     7261 tgaatatcag gcaattcctg taagagctgc ggatgccatt tttctgcaaa acctgtacga
     7321 ggcggaagat cacccgactt gccatgtcct ggaaagtaga aatccatagg caaaacagca
     7381 aaataacctg aattgtaaaa ggtatcttca tccacaccta gccagtcccg caagcggtca
     7441 ccacttttat ctttccagta aaggcctgct tcttgagttt taagtcccgg agcctgaccg
     7501 atgatattga tgcgagcagt ttttggcgct gcaaagagag gctcaatgcc acgctctgta
     7561 tagctggcat tctgtgaatc cgccatgata gcctgtttga ttctttcgat ttgagacatc
     7621 tactttccct ccaaaaagaa gtccaagcat aagatctcag acttctatcg ttttatttga
     7681 tcaattcgta aatagcttcg gcatagattg ctgctgctcg gaagagatcg tccaaggcga
     7741 taaattcatt ggcttggtgc atggtatcaa ttgagtctgg gaacatagca ccataggcaa
     7801 ctccgcgctc taacaagcga ccaaaggttc caccaccgat gacttgttca tgacctttaa
     7861 agccagtttg tttttcatag atattcaaca aggtttgcac aagtggatct tccattggca
     7921 catagtgagg cgtgtgaccg tgttcagaca ggctaacaga aacaactggc aagttttcaa
     7981 ggattgactt gatttgttct ggacttgttc cttttggata gcggatgttg agggcaatgg
     8041 tattatcagc acttgtttca tcgaagcgga agacgccggc attcatagaa agagcaccca
     8101 tcttttcatc cacatgagca atcttaagat tttcaccctc atgatcgttc aagagaattt
     8161 tacctgcgat gtcaaggtag tctttggctg gaccagcaaa gccaaactga ctgaggaaga
     8221 gggcaaggta agttgcgcca ttgacacctg aagcaggcat agcaccgtgg gctgatttac
     8281 caatgatcgt caccttgtat ttgccagctt cttcttggag ttctcctcta agtttgtgtt
     8341 ctgcaacaaa ggcatctagt ttagcttgca agtcagccaa gtcacctgaa acgactgctg
     8401 ttgctgattc tggtaccata ttttcacgta aaccacctgt aaagctgtga agacgggcaa
     8461 cacctgtatt ttctcctgca aagtggaggt attccgtgat atttcctttt tcaccattga
     8521 tgattggaaa ttcagcatct ggtgagaaac cgaaatctgg tttggcaagt cctacgtgct
     8581 caaagtagta gtccatgtct gcccagcctg attcttcgtc tgttccaacg atgaagcgaa
     8641 ctttcttaga agttggaaga cccaattctt tgatgatttt caaaccatag taacaagctg
     8701 ttgtaggacc cttatcgtcc gaagccccgc gcgcataaag gcgaccatct ttgatagttg
     8761 gtgtgtaagg gtctgtgtcc caaccgctac cagcaggcac cacatccata tgggcaaaga
     8821 ttccgagaac ttcttctcca tcaccaaact caaaatgtcc tgcatagtta tcaacattct
     8881 tagttgggta gccatcgcgg tctgcgattt caaggaattt ctccaaggct tttactggac
     8941 caggcccaaa tggatgctgg gcatcagcct tgctgtcatc acgttctgaa ttgatttcca
     9001 aaaggctaaa caagtcagcc aagaggtctt ctttgcgttt ttctacttct gctgtaaaat
     9061 caattgctgt cattttttct tctttctatc ttttctcgat gatttcatct actggcaagc
     9121 ggtagcttgg ttccaatttt tcgtctgtat aacccactgt gatcaagagt tctgggcgga
     9181 aacggtcttc gatttccaaa acttcattaa cttttgattt gtcaaaacca agaataatgt
     9241 tagaaccaat tccttggtct gtcaatgcaa gaaccaagtt catggcaacc aaacctgcat
     9301 tgagagctag gtagtcgctg acttgttgtt cattgtagcg tgcaaattca gctggcaaat
     9361 ttttcatgaa gtattgaagt tgttcttctg agaaattgtt agcaccacca actcgggcaa
     9421 tcttacgagc gcgtttggca aggtctgtat ctgtaaatag ggcaatggtt acaggtgctg
     9481 atgatacctg ctcaaagttt gaaccataag ccaactttgc cagttcagca tttttctcac
     9541 gaacaactac aaatttccaa ggctggctgt tgtgggcgct tggcgccaag gttgcaattt
     9601 cgatagccgt acgcacatct tttggatcaa caggcttatc agtgaaatgc ttggtcgcat
     9661 gacgtttttt atttaattca agaaatttca taatcgattt ccttttctaa ttctactgct
     9721 tccattctac cataatttga aagagcttgc agggattttt catgattaag tttttctatc
     9781 acagacataa aagatatata ttggtttatc aggaaagttt ctgataaact agcaagtact
     9841 ttacatttga atggagacag tatgaaaaaa tttagcctat tactagctat cctaccattt
     9901 ttggttgcct gtgggaatca agctacaccc aaagagacta gcgctcaaaa gacaatcgtc
     9961 cttgctacag ctggcgacgt gccaccattt gactacgaag ataaaggcaa tctgacaggc
    10021 ttcgatatcg aagttctaaa ggcagtagat gaaaaactca gcgactacga gattcaattt
    10081 caaagaaccg cctgggaaag tatcttccca ggacttgatt ctggtcacta tcaggctgcg
    10141 gccaataatt tgagttacac aaaagagcgt gctgaaaaat acctttactc gcttccgatt
    10201 tccaacaatc ccctcgtcct tgtcagcaac aagaaaaatc ctttgacttc tcttgaccag
    10261 atcgctggta aaacaacaca agaggatacc ggaacttcta acgctcaatt catcaataac
    10321 tggaatcaga aacacactga taatcccgct acaattaatt tttctggtga ggatattggt
    10381 aaacgaatcc tagaccttgc taacggagag tttgatttcc tagtttttga caaggtatcc
    10441 gttcaaaaga ttatcaagga ccgtggttta gacctctcag tcgttgattt accttctgca
    10501 gatagcccca gcaattatat cattttctca agcgaccaaa aagagtttaa agagcaattt
    10561 gataaagcgc tcaaagaact ctatcaagac ggaacccttg aaaaactcag caatacctat
    10621 ctaggtggtt cttacctccc agatcaatct cagttacaat aacactcttc gaaaatctct
    10681 tcaaaccacg tcagctttat ctgcaacctc aaagctgtgc tttgagcaac ctacggctag
    10741 cttcctagtt tgctctttga ttttcatatt gagtataaga tatattaaaa gcgatgggat
    10801 cagccaatcg cttttttagt ttgcattggt tattttagga aactcttaca aaaaatcaaa
    10861 aaaattcttc acatcctcta catacctatg cttttctatt aagcttgcta atagttccag
    10921 attgtgcctc tgatagttat cttcacgacg tagttcttca tacatttcga taaagggaaa
    10981 acccttggat ttggcaaatt ctaactcagc ctcgtaatca taagcgactt tacgaaatag
    11041 gatatttacc aattccccat cttcaacctc tatcacggca tactgggcac ggtgattttt
    11101 taacgcctcc caattaaaat agggcatgcc aatcgaccct ggattgatga tttgttgccc
    11161 ttgactacca taacgaagca actacttgtg aacatgacca taaactgcca cgtccgtttc
    11221 cgcatctagc agttggtcaa atttctctgt atcattctca actagcaagt caccaccata
    11281 gtttttgtca ggtaaattat gagagataga aaagcgcaat ccgtcaattt ctttcttttc
    11341 cagcaaaggc aagcttcgta gccagacaat cgttgcagga tccattcgct ccatcaaata
    11401 ctgtgtcata cgcaagagct gaacttcctg tgggtcttct aagccatatt gcccatctaa
    11461 agcctcaagg acacaatcat cccaattgcc tcgaacactt gccgtgatag gaagttcctt
    11521 taacagagcg accaagtcat ttgcacctgg accagggagg aaaatgtctc ccatgagcca
    11581 atattcactg gctccttgag ttttagcatc tgcaatcact gcttctagcg ccgtcgcatt
    11641 gccatgaaca tctgataaaa ttgcgatttt atggtttatt gtggtttcct tccgtttggt
    11701 aatctactct ttcttccgcc acttgaagca agatttctgt ttcctgcggg ttcaacttgg
    11761 tttgcacagc ctctgctact gctcttggta ctccaacttc gacaatctca tccacactgg
    11821 cttccttgat tttggtcaga gacttgaagt gcctcatgag attttgcttg cgtttaggtc
    11881 ccagaccatc aatcccatcc aattgagatg agaaagaatt tttggagcgc agttggcggt
    11941 ggaaggtaat ggcaaagcgg tggacctcat cttgaatgcg ttggaggagg aaaaattcct
    12001 gagaattgcg agacaagtcc accacttcca gcggatctcc aaagagcaat tcatgggttt
    12061 ggtgcttatc attcttttgc agacctgcaa ttggaatatc caagcccagt tcttcttgga
    12121 tgacttgctt agcaatattg acttgacctt gccccccatc aatcacaatc aaatctggag
    12181 gagtcaaagc ctcacgctgt actcgaccat agcgtctgcg aatgacctct cgcatactgg
    12241 catagtcgtc tggcccgact acggtcttga ttttatactt acggtaatcc ttcttactag
    12301 gcttgccgtt gacaaagacc accatggctg aaacaggact agttcccatg atgttggagt
    12361 tatcgaagga ctcgatgcga actggggttg ggatttgaag caagcgtcct agattttcaa
    12421 tagctccttg ggtcttctcg acagatttct ctagcaggtt gaacttctgc tctaggctaa
    12481 cacgggcatt ttttatagct aaattgacca gctgcttttt ctctccacgt tgaggcttaa
    12541 gaatcttgga atccaccaag gccttgacag cctcttcgtc aatatcttgc ggaatcagta
    12601 cctcattggg aactagatgg gatttttctt gatagaattg tcctacatag gtcaaaaaat
    12661 cctcatctgg atcattgaag taggggaaga gattgacatc gcgctcgatg agcttaccct
    12721 gacggacaaa gaaaacctgc acacacatcc agcccttatc cacatagtag ccaaagacat
    12781 cgcgattttg gagatcctta gccatgaccc gttgcttggt tcgaagcgtt ccaatagcct
    12841 gaatcaggtc acggtattcc gccgcacgtt caaactccat agtctgggct gccgctgcca
    12901 ttttgccctt tagctcatca atgattttgt tatcctgccc tttcagaaaa tcagaaacct
    12961 cctgggccat agacttgaaa taaatctcat ccttcttaca gatggtgtgg gccatgcatt
    13021 gaccgatatg gtaataaaaa cagaccttag agggcgggtt ggtacactta cgaaaaggga
    13081 atatccgatc cagcaaccgc ttgatttcat tggctgcccc cacatcggga tagggtccaa
    13141 aataaagacc tccgtccttt ttgacctgac gagtgataat caagcgtgga tagcgctcat
    13201 tggtgatttt gatgaaagga taggacttgt catccttgag catgatattg tacttgggct
    13261 tgttttcctt gatcaggttg atttctagga gaagtgcctc aatattagac tccgtaacaa
    13321 taaattcaaa atccacaatt tcagacacca gagcctctgt cttggtatca tgacttccac
    13381 gaaaatagga ccgtactcga ttacgcagat ttttagcctt tcctacatag ataatggtgc
    13441 catttttatc cttatgaatg tagcaaccag ggctggtcgg caagagctct agttttgatt
    13501 taatcaagtt gttcatagtt ccattatagc aaaaaaagta gggaaagata gtaccctact
    13561 cactagtctc atatattttt cggaggtttg caacatcctc ttcctggata ccataaaagg
    13621 aacctgctgg ttgcatgaca gacttctcat ctgtatggtc caagccaatc gcatgaccta
    13681 actcatgttc tgccgtatgg acaaggcgtt cataggagta gccatagtat ggattggaca
    13741 aataataatg attcaaccgc accgttacgg acaagaattg ccctgttaag agattagttt
    13801 gactttccgc ctctcctgcc acaggagtgc ctccgtcgtt catctccgta gccgtaatat
    13861 ccgccttact agactcagtc acgagttcaa agttaaaagc accagtttga ttccagttct
    13921 ggattgcttc taaataagcc tcttgaaagc gtgaatccat ctgcggatcc aggtaaatac
    13981 gagcagaggc ctgtgcccac cttccttcag aatgctggtg actatctgtc tggcttaact
    14041 taggcagttg ccctgttttc tcccattggt caatactttg ttgaccaatt tttactgacc
    14101 gttctgcttg ctttagcgct ccttgaaaat ctccgttcag ataccagaga agtccgaaag
    14161 caagcacaca taagagcaca actatccaaa ccagacgcca aaacaaacgc cacacaaaag
    14221 aaaagaaagc ccctatcaaa cgaaaaagcc agcgcatgtc tctccacctt tctagcaaat
    14281 aagaattatt ttactaaaag aagctaaaag aaatctaaat actgactttt tcatcttaac
    14341 gtcctactat ttttttgagt aactggatag ctttgtattt taaaggagat ggatggtaac
    14401 ggaaagtacc tgctttgcgt acaatatagc cattaaaatt ctgtttaaaa cgcaaaacac
    14461 catcacttcc atcaaaaatc ccttgaatgc ctaggaagtt gtatttaggt attccacgtt
    14521 ttatgctttc caacataaca tatttttgaa gcagggcagg ggcatagaac ttattaaact
    14581 cagtgtagga accactaaag agataagtcg tttcctgagg catataaaca aataaactcc
    14641 cagctaaaac aatatcttct tctccatatt tttcaatcaa gtctcgcgct tctgcttttc
    14701 gaacttcaaa cgtttcaaat tgactagaat attctctcag ttgattttgt tttttctcag
    14761 aatgaggatt tttactcaaa tcaagtcgca acttgtccaa gttttcttct agtttacttt
    14821 gttcaccttg caatttgctc atatagtccg aaaaatttaa gcttgctatg agaaactccg
    14881 cttgttctcc aaaagtatca taaaaatgct cataatattc taaactttta tcactatatt
    14941 ctctacgttc agaggtttct tttgttatat tcttaaaaat cgatagttct tcacgtttta
    15001 actttttcaa ccgaatgcca aaggtttcag cctttttcac caagggttta ccctttttgc
    15061 taaaactttt aagcaaactc ttttcagtta attcagttaa atctttatag tataaccaat
    15121 ctggttctcc acctgggtaa cctgttgtta agccatcaaa ttgataacct aaatcagtca
    15181 aaccttgaat aatacttttt ttctcagcat ctattggatt accttggcta tcaaaagttt
    15241 gataagtttc ataaggtttt acaagcaact ctaatacacc attttgcttg gcatattctt
    15301 ttaactctgc ataaaaaact ggaagagcat cttgttgggt ataaattggc cccgaattga
    15361 gttccatatg cagaccaccc agcatgggca agctataaac cagagctgca acttgaattt
    15421 ctccttcttg tttcaaagca agataaacaa ttcgagcccc tcttttttct agcaaatccc
    15481 ccatctggac agattgcata aaggaacgag aagaaacctg atcagaataa gtctgaaact
    15541 cttctttcgt gagtgttgtt agtgccatat acttactttc tatgtttttt tcttaatgtt
    15601 ttacggaaat caagagcaag tcttaacaga ggatagagag gatgagtggg cattgtaaat
    15661 tcacccaagt attcttcaat cgttggatta aatttttcct taaaatgata aagtccacca
    15721 ttgagagagt tttcaacacc acctaaattt tgccagacca tacctcgctc aaaggcatag
    15781 cgagccgttt cataccatgt taaaattggt gcattgtaac gtttaaaatc atcatccata
    15841 ccagcatata tattaacaga ggtagtacca aattccaaac tcaaagtagc cgctaaagga
    15901 actctcgctt gacctacatc tatatattcc tgcaagaagg tcaattcctc taacaaacgt
    15961 tctttttcct tcttctgcgc ttctactttt gaagttcgag tcgactcagt aaaggtctct
    16021 tccaaggctc tatttttcgc taactgttct tctaattctt gcgaacgttt agaaacatcc
    16081 aaggtggcca aggtgatata ggccttgtcc ttaaaattat ctaacaattt tttataatag
    16141 gcttcattcc tcaaatgaat ctcttttcgc ttctcagttt ttttcatcaa ctccgaaaat
    16201 gaatctaata gttccagtcc accatattga atctcaagcc ctttgtttcg tgctgttcga
    16261 atagcctgtt ttgttgactt ggaaagttta tcttcttcaa aattttcctt gtatattttc
    16321 gcctgaatac gaggttgaat ggtgtctccc atttcctccg tttttcctga ccaccttact
    16381 cccatttgtt gcaaactatc aataatagcc agattttcag gaaattctgt cttttcctga
    16441 ttgattaaac tttgagatag gcaaatactt gggtcaaaag tcacaaaaac cgctctctta
    16501 ctgcgagcat aggacttaat agactgaatg gcaaaattca agagttcttt atccccataa
    16561 tccaatatag gtcctcttgg gatgtaaaac attttatagc ctagcggaag agttctaatc
    16621 aaaatactag ctgtcgccag taatttttct tccctgtaaa caccaaactt ctcatgttgc
    16681 caattagact taacttcctc ccaagcacta ctttgtaata cattggctaa ttcatgttct
    16741 ttgacaaact gatcatattc taatgtggga atgccaattt gataacggta catttcctac
    16801 tctctttctt ccagtatttt attactattc tactactttc gtttgaaaag tctagtgaaa
    16861 acatatttta ttttagttct actcccccta tgctgaagtt caaactaagt cagtctctct
    16921 tctaatttga aatcaattgg tagggttgtt aaaaaaactt ccaattgttt ttcccagtag
    16981 taccactcgt gagttccagc actatggcta taggtcacat caaaacctag ttttttgaga
    17041 tttttcactg cgagattatt ggcttcgtac aagaaatcct gttcgccaca ccacgcccaa
    17101 agtttggtct ttttatccga ttttttagcc agactttcaa gagaataggg actagttgtc
    17161 cagtctctaa tctctccaaa aacacctctc cagtaggctg gacttcccag attttgactt
    17221 tcaggagaaa agttttgaaa gctgagggca cctgaaaaac tagctgcatg agaaaaacga
    17281 tttgtcgtaa gagccagttt gaagcagccg tagcctccca tagaaagacc agcgataaag
    17341 gtcttttcac gcttgctagt catattaggg aagaagcgtt tcagaacctg tggcaattcc
    17401 tctgctagag ccatgtagta gtcaaaacca tactgggtat cggtgtacca accattgctg
    17461 gtattgggca taacaacgat gagattagtt cctcgaagca agcgttctac attggtccgc
    17521 ttaagccaac tattatgatt tccagacatc ccgtgcaaaa ggtacaagac gggaatatct
    17581 tcacactctg gttcttccac tcgattggca tcagggtaga ggacattcac cccccactcc
    17641 atatccaata cttgtgagta atactcgatt ttcattactg ccatgatttt ctccttctat
    17701 ttttctataa gaaaaagccg aaactcgact ttctctctgt ttatttcaac gattattttg
    17761 cttccatgac tactggtaaa atagctggac gacgcttggt ttggtcaaag agatacttgg
    17821 tcaaattatc tcgaaccttc cctttaagat ctgcccagtc aaagtcatct ccttgaagat
    17881 actcttctac cgtttggtta atcaattctg aactttcacg gagaatatcg cgactcttct
    17941 tgagataaac aaatccgcgc gtatgaacac gagccctagc cacaattttc ttctcacgac
    18001 ggttgactgt aatagccacg atgaaaattc catcctctga caagacctta cggtcacgaa
    18061 gaacaacatt tccaacatca ccaatggcat tcccatcaat caagatatct cctgctgaaa
    18121 ccgatccagc tggaacaaag tctccattct cgtaagccat ggtcgtcccc tttttaggaa
    18181 tgaagatgcg ttctggcaac atcccaactg ccatggcagc cttagcgtga gcatccaact
    18241 cacgatactc cccttggaca gggaagaggt acttaggttg caaaagattg atcatcagct
    18301 gcaaatcacg cacatttccg tgccctgata catgtaaact ttgggtaatc aatttgacaa
    18361 ccccacctgc ctgataaatc atattttcca cacgcgcaac aaaggcttct ttagcaatag
    18421 acggagccgt agcaatatag accaggtccc catccttgat ttctacataa cgatggcgac
    18481 caatcgacat cttacgaagt ccattgatag gttcacccat acgacctgtc tcaagaataa
    18541 tcaactcatg gtcttcaaag cgagacatat ctttaggctt aatcaagaga atttcgttgg
    18601 ctaaagacaa cttcttaaga cgaatcgctg tgcggacgat attttcaata tcaaatcctg
    18661 tcaagacgat acgtcgacct gttttatccg cagcgtcaaa aatctgctga atacgagaaa
    18721 gattactgga aacagctgca acgatgatac gaccttccca gtcagcaata gtttgggtaa
    18781 tttcatccct aacttcactt tcactagcca cctgaatatt gctgtctgca ttggccgaat
    18841 cactgaggag agccaggacg ccgtcacgac caatctctgc caaacgagca aaatcagttg
    18901 cataagattc actagccgtt tggtcaaatt tgaagtcacc tgtataaacg atgcttcctt
    18961 ccgatgtctt caagacaatt cccagactct ctggaacgga gtaagtcgta gggaagaagg
    19021 aaaccactgt cccaccaaaa tcaatctccg tattctcatc aatgacatgg aaatcattaa
    19081 atttcttaac ggcatcattt cctttgacaa agagctttgc caactcaatg gtcaactcag
    19141 acccaaatac aggaacttta gcctctgcca agagataagg tagagcacca atggcatccg
    19201 catgcccgtg ggtcaagaaa accccagcaa tacggtcgct attttcaaaa aggtaatcca
    19261 tgtttggaat caccacatcg acccctaatt gttcattttc aggatatttt aaccctacat
    19321 tcaaaacaaa aatggactct ccaatttcag caatgtacat attttttcca ttctcacgca
    19381 caccaccaag tgttgttaaa ctgatattac tcatttttcc tccaaaatct tagtttatct
    19441 tgattatagg gcaactctac cctcttattc taaaagttct gatactttca gccgaatctt
    19501 ttcctaccat tataccattt ttttgatgat tttcaaaatg aaggtctgtt ttcaaaaaag
    19561 agttgatttc ccaactcttt cctaacttct actctttttc catcaaaaaa tcataaattt
    19621 cttgcaccgt acccagcgcc atcatttctt ggtctttaac ggtttgtttg agattttggt
    19681 aaatttgact ttctcctatc tcccgctttg gcgcctcttt attgactgtc atcacaccat
    19741 cttttatcgt cacaaagcct agttcttcaa atacttgaat catcttgacc agcaagattt
    19801 gttgaatatt aagatatgca gccaaatctt tcagcttgta gcgaatatca aactctggga
    19861 actggtaaat agtcttgtac aatttagcaa actgatctct agtcccataa cctgtcagat
    19921 aataggtctt gtcaatatca tttttgaaat agacagcaga gaaatgctgt tcctgaaaaa
    19981 tggtcttcag ctgagtaata tcctctggaa tgttttttac gacaacagct tcactagccg
    20041 caagatttgg cagttctcca ggaaaatcca agactggaac accttctggc aagactgcat
    20101 tttttccacg aatgttaaaa agttgaacac cttccactcg cgcatccacc atcatcaact
    20161 ggagggcagt ttggccattc cattggttga cagacaattt aaccgctaac tctagattct
    20221 tggtttgaga aaactctgtc gcccatctgc cttgaccaaa ggctaccact tcaaaactcg
    20281 cctcaccctt ggaaattttc agctttagat gggcattacc tgcccccata gtacgagcac
    20341 tttcgacctg aaaattcttg atataaaaaa taggtttctg attatccatt ccaaaaggag
    20401 ctaaacgttc aaaacttttg accgtttcca agctaagtgc ctccaaatcc aactcttcat
    20461 ctaggtttaa cttattcttg ccaccagcat ctgcaccttt ttcacgaaca taatcttcca
    20521 aaacctgaga taaatctgag agttgctcaa cttccagcgt catacccgct gcacctgcat
    20581 gacctccaaa ggcgatgaag aggtctcgat ggggatccag agcttcaaaa atatcgaccg
    20641 cttccacact acgagcactg cccttggcac gaccgtcttc tatattaaga acaatgactg
    20701 tctgtcccaa ttcttccaat aaacgaccag ccacgattcc tagaacccca ggattccagc
    20761 cttccttggc caagacctga accttcttct caggatccac catggtcttg gcttcttcat
    20821 agatagactg aacgatttcc ttgcgctctt cgtttttctg gtgaatcata agggcaatct
    20881 catgcgcttc ctcatcatca aatccagtca acaaatcaat ggcaggattg ggatcatcca
    20941 agcggcccaa ggcattcaaa cgaggagcaa tctggaaacc aaccgtttct tctgttactt
    21001 cgttggcagc aatcccagcc atgtccagca tttcttgcag accaatgcgc tgggtatgac
    21061 ccaacatttc cagaccatat tgaactaaga tacgattttc atccgtcaga ctcaccatat
    21121 ctgcaatagt tccaatagcg accaaatcaa gcaattccac ttgcacttct tctaacaggg
    21181 cacaagccaa cttgaaagca actccacaac cagccaaata tttaaaagga taatccgcat
    21241 ctggatgttc aggatggaca atagcataag catctggcag ggtttcaggc atggaatgat
    21301 ggtctgtcac aatgacatct actcccatag actgagccaa tgcaatagcc tcatgaccag
    21361 caaccccatt gtccaccgtc acaatcaagg aaatcccttc ttgctcgata aagtatttat
    21421 aaacactagc attagggcca tagccatcgg taaaacgatt tggcaggtaa actcggcact
    21481 cagcaccaag ttgttccaaa ctttccttca caatagaagc cgaagtcatg ccatccgcat
    21541 cgtagtctcc ataaacgaga atattttccc tttcttcaat agcctgacga atccgctcca
    21601 ctgccttgtc catatcatgg agcagataag catcatgtaa gtcctccaag gaaggttcta
    21661 aaaacttctt cagactttct tggtcctgaa tccctctctc aaacaataac cgagccacct
    21721 caggacccag tccagccttc ttggctatct ttgtaaaatc cgcatcttct acctgcaggg
    21781 caaactgcca ttcataagta ggtgttatca aaaagacatc cactctttct aagaattcta
    21841 ttgcttcatt ataacatgat ttagacgttt tctctatcca gattgctttt ttataacatt
    21901 ttatagtata ttgaaactag aatagtacac ctctacttct aaaatattgt tagaaatcga
    21961 tttgactgtc ccgatcgatt tttcctgttc ttatttcact atagtgaatt ttttcagata
    22021 cgatttgaca agaacattct tttattgcag aatcgtgtta taaaatctaa cataaaggag
    22081 ttctctcatg gctttagtag aatttaaaaa cgtcgaaaaa tattacggag actaccacgc
    22141 actccgcaac atcaatctcc gttttgaaaa aggacaagtt gttgtcctgc ttggaccttc
    22201 tggctctggg aagtccactc ttatccgtac gatcaatggt ttagaggctg ttgacaaagg
    22261 aagtctccta gtcaatgggc accaagttgc tggtgccagc cagaaagatt tggtacctct
    22321 tcgcaaggaa gtcggcatgg tttttcaaca ttttaacctt tatccacaca aaacagtgtt
    22381 agaaaacgtg acacttgcac ctattaaagt tctaggaatt gataaaaaag aagctgaaaa
    22441 aaccgccaaa aaatatctgg aatttgtaaa tatgtgggac aagaaagatt cctatcccgc
    22501 catgctatct ggtggacaaa aacagcggat cgccatcgct cgtggtcttg ctatgcatcc
    22561 ggaactcctc ctctttgatg aaccaacatc tgctcttgat cctgagacta tcggagatgt
    22621 tctagcagtt atgcagaaac tggcgcgtga tgggatgaac atgatcatcg ttacccacga
    22681 aatgggcttt gctcgagagg ttgcggaccg cattatcttt atggccgacg gagaagtttt
    22741 agtagatacg acagatgtcg ataacttttt tgacaatcca agcgaacctc gtgcccaaca
    22801 attcctcagc aaaattatca accacgaaag tgacaaagtc aaataaggag gcgcctatga
    22861 aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg ctgccaaacc
    22921 agtccaggct gatacaagta tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt
    22981 caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt attctggtat
    23041 cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc gctatgtgcc
    23101 ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata tggatatcgc
    23161 gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc cctactacac
    23221 agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg aggacctaaa
    23281 cagcaaaacc atcggagtcg cccaaggctc catcacccaa tgcctgatta ctgaactggg
    23341 caaaaagaaa ggtctaacct ttaaatttgt cgaacttggt tcctacccag aattgattac
    23401 ttcccttcac gctcatcgta tcgatgcctt ttccgttgac cgctctattc tgtctggcta
    23461 cactagtaaa cgcacagaac tactagatga tagtttcaag ccatctgact acggtattgt
    23521 taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta ctaaatggag
    23581 caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat ctagccatac
    23641 tgcagattaa ggaggacacc ccatgacaga tttatcatct tggacagcct attttcagga
    23701 ttttggacaa tttttcaatg gtttcctctt tactcttgcc ctagcgattg gattctttat
    23761 cctcgccatg gtcttaggaa tcttctttgg tgccatgtca accagtaaac gtccaatttt
    23821 acgcatttta gctcacatct ttgtggaatt ttaccaaaac actcccctct tggtgcagtt
    23881 tgttattgtt ttttatggcc tacctcttat cagtgaccat actatcatga ttccaattta
    23941 ttggacagct gttctctgcg tgggactcta tcacggcgct tatatcgctg aggttattcg
    24001 ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc aaggttttac
    24061 ctatatcagt gccatgcgct tgattacctt gcctcaggcc ttccgcatca ttctccctcc
    24121 attgaccaac caaattgtta acctcatcaa gaacacatct actgtagcta tcatctctgg
    24181 agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact atatcccagc
    24241 ctttttaggt gctgctcttc tctactttgc cctatgcttc cctgttgccc agtttggtcg
    24301 caagatggag caagccaata aaaaagccta ttcactttag gaggttacta tggaatctat
    24361 tttagaaatt ttaaccccag ataacctagt ctttatcttt aaaggatttg gcttgaccct
    24421 ctatatttct ctgattgcca tcatcctctc tactatcatc ggtaccgtgc ttgccgtcat
    24481 gcgaaatgga aaaaatccta tcttacgcat tatttccagt atttatatcg agtttgtgca
    24541 caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa tgaagtccac
    24601 accagcaggt attacagcct ttactctctt tacatcagca gccttggctg agattattcg
    24661 aggcggtctc aatgccgtag acaagggaca atacaaagca ggaatgtcac aaggcttcac
    24721 ctcagcccaa atcctctact acatcattct cccacaagcc agccgcaaaa tgctaccagc
    24781 catcatttct cagtttgtta ccgtgattaa ggataccagt ctcctctact ctgttatcgc
    24841 cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttatttcg aaccagagca
    24901 ggtcttcagt ctttacatcc tgattgccct catctacttc agctttaacc tagcaatttc
    24961 tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataat actcttcgaa
    25021 aatctcttca aaccacgtca gcttcacctt gccgtatata tgttactgac ttcgtcagtt
    25081 ctatccacaa cctcaaaacg gtgttttgag cagcctgcgg ctagcttcct agtttgctct
    25141 ttgattttca ttgagtataa acagccactc tccagttcaa ttggagagtt ttttgatgag
    25201 agtagatatt tatagacctc aatttgacac catacatttt ctatgataaa atgtaacaaa
    25261 ttcatttaca acaaatcgaa atctaaatga aatggaattt tctatgaaat cagtcaaagg
    25321 actactcttt atcatagcta gttttatctt gactcttttg acttggatga acacttctcc
    25381 ccaattcatg attccaggac tagctttaac aagcctatct ctgactttta tcctagccac
    25441 tcgtctccca ctactagaaa gctggtttca cggtttggag aaggtctaca ccgtccacaa
    25501 attcacagcc tttctctcca tcatcctact aatctttcat aactttagta tgggcggttt
    25561 gtggggctct cgcttagctg ctcagtttgg caatcttgcc atctatatct ttgccagcat
    25621 catccttgtc gcctatttag gcaaatacat ccaatacgaa gcttggcgat ggattcaccg
    25681 cttggtttac ctagcctata ttttaggact ctttcacatc tacatgataa tgggcaatcg
    25741 tctccttaca tttaatcttc taagttttct tgttggtagc tatgcccttt taggcttact
    25801 agctggtttt tatatcattt ttctatatca aaagatttcc ttcccctatc tagggaaaat
    25861 tacccatctc aaacgcttaa atcacgatac tagagaaatt caaatccatc tttgcagacc
    25921 tttcaactat caatcaggac aatttgcctt tctaaagatt ttccaagaag gctttgaaag
    25981 tgctccgcat cccttttcta tctcaggagg tcatggtcaa actctttact ttactgttaa
    26041 aaattcaggc gaccatacca ataatatcta tgataatctt caagccggca gcaaagtaac
    26101 cctagacaga gcttacggac acatgatcat agaagaagga cgagaaaatc aggtttggat
    26161 tgctggaggt attgggatca cccccttcat ctcttacatc cgtgaacatc ctattttaga
    26221 taaacaggtt cacttctact atagcttccg tggagatgaa aatgcagtct acctagattt
    26281 actccgtaac tatgctcaga aaaatcctaa ttttgaactc catctaatcg acagtacgaa
    26341 agacggctat cttaattttg aacaaaaaga agtgcccgaa catgcaaccg tctatatgtg
    26401 tggtcctatt tctatgatga aggcacttgc caaacagatt aagaaacaaa atccaaaaac
    26461 agagcttatt tacgaaggat tcaagttcaa ataagtaatc atactctcct gggatttccc
    26521 aggagagttt tttatagcca aatgaaataa gaccggaacg atggaatttt ggcattcaaa
    26581 tcaattgata agaatatt
//
LOCUS       DAVHIU010000018        25206 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVHIU010000018 DAVHIU010000000
VERSION     DAVHIU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.018"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            102..414
                     /locus_tag="VVN06_000619"
                     /pseudo
     CDS             102..414
                     /locus_tag="VVN06_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            418..1134
                     /locus_tag="VVN06_000620"
     CDS             418..1134
                     /locus_tag="VVN06_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5562218.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1264..2079)
                     /locus_tag="VVN06_000621"
     CDS             complement(1264..2079)
                     /locus_tag="VVN06_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU5562219.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2076..2981)
                     /locus_tag="VVN06_000622"
     CDS             complement(2076..2981)
                     /locus_tag="VVN06_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU5562220.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3393..5522
                     /locus_tag="VVN06_000623"
     CDS             3393..5522
                     /locus_tag="VVN06_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU5562221.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            5509..5958
                     /locus_tag="VVN06_000624"
     CDS             5509..5958
                     /locus_tag="VVN06_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU5562222.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6017..6289
                     /locus_tag="VVN06_000625"
     CDS             6017..6289
                     /locus_tag="VVN06_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5562223.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(6643..6834)
                     /locus_tag="VVN06_000626"
     CDS             complement(6643..6834)
                     /locus_tag="VVN06_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562224.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            7262..8020
                     /locus_tag="VVN06_000627"
     CDS             7262..8020
                     /locus_tag="VVN06_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562225.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            8022..10010
                     /locus_tag="VVN06_000628"
     CDS             8022..10010
                     /locus_tag="VVN06_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562226.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(10053..10631)
                     /locus_tag="VVN06_000629"
     CDS             complement(10053..10631)
                     /locus_tag="VVN06_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5562227.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGGEYVSVSTPKDTEEAA
                     VSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLLTNKIFLKNPLKALVEEKYGIEY
                     EEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRIPATVLIVGVALLLTGYTSARL
                     GKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(10627..11502)
                     /locus_tag="VVN06_000630"
     CDS             complement(10627..11502)
                     /locus_tag="VVN06_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU5562228.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPDKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHP
                     YASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWM
                     ANLNLKFSVRNKSRN"
     gene            complement(11960..12358)
                     /locus_tag="VVN06_000631"
     CDS             complement(11960..12358)
                     /locus_tag="VVN06_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562229.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            12480..12560
                     /locus_tag="VVN06_000632"
                     /pseudo
     CDS             12480..12560
                     /locus_tag="VVN06_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(12686..13168)
                     /gene="greA"
                     /locus_tag="VVN06_000633"
     CDS             complement(12686..13168)
                     /gene="greA"
                     /locus_tag="VVN06_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5562230.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(13239..14894)
                     /gene="mltG"
                     /locus_tag="VVN06_000634"
     CDS             complement(13239..14894)
                     /gene="mltG"
                     /locus_tag="VVN06_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5562231.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADESQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATEIVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDKIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(14975..15469)
                     /locus_tag="VVN06_000635"
     CDS             complement(14975..15469)
                     /locus_tag="VVN06_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562232.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(15466..15930)
                     /locus_tag="VVN06_000636"
     CDS             complement(15466..15930)
                     /locus_tag="VVN06_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562233.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(15940..17274)
                     /locus_tag="VVN06_000637"
     CDS             complement(15940..17274)
                     /locus_tag="VVN06_000637"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU5562234.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(17285..17902)
                     /locus_tag="VVN06_000638"
     CDS             complement(17285..17902)
                     /locus_tag="VVN06_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5562235.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(17950..21048)
                     /locus_tag="VVN06_000639"
     CDS             complement(17950..21048)
                     /locus_tag="VVN06_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5562236.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(21141..22307)
                     /locus_tag="VVN06_000640"
     CDS             complement(21141..22307)
                     /locus_tag="VVN06_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5562237.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(22315..23409)
                     /locus_tag="VVN06_000641"
     CDS             complement(22315..23409)
                     /locus_tag="VVN06_000641"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5562238.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(23567..25009)
                     /locus_tag="VVN06_000642"
     CDS             complement(23567..25009)
                     /locus_tag="VVN06_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562239.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIART
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
BASE COUNT         7421 a         5226 c         4913 g         7646 t
ORIGIN      
        1 ctaaaatctt atcagtgaga agatttctta aagtttattc tttgtagttt catctgtatt
       61 agtacataat ttaacttaga gaaaatagga ggactaatcg aatggaatta aaagatttta
      121 cagaaaaaga acaggaaatg attaagaaaa ggcttacaat gtctaatatt agtgacaaag
      181 aaactactga gaagattctg gcgctagtcc cacaagactt gattaagcga atcccgtttt
      241 ttgtcagaaa acatgctaca acacgtacga ttaaacgcat ttcaattgaa taccctgaac
      301 tctacgctgt agctcaaaca agtggtgaga ttccagaaaa aaaaacgcga agaattgcgt
      361 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
      421 aaaaaatatt ttattggcgg tttgggaagc aatgcctatc atagcaagga ttttcttcaa
      481 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
      541 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggt
      601 cattctattg gaggcgattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
      661 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
      721 ttagaggaaa ctaaaaatta tatcaaatct caaattattt cggacttaga tgttcttact
      781 tctaaagaaa aatctgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
      841 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
      901 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
      961 ttgtttatta gtcctcgcta tcctaatgaa gctacatgga gagaggaagc cctaaaagaa
     1021 ttgccagact attttgatac tattttttta gaaaactttg gccatgagct ttatactcaa
     1081 gcacctaaag aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact
     1141 tgtattgctg gaaatggtat aagatgaaaa tctacaagtc actaattttc atataaccaa
     1201 aaaacgaaca ggctcttttt ctgttattca gaagagaact tgttcgtttt tatcttgatt
     1261 ttcttaaaaa gctctatata catagggatt ttctggttta tcgactttgg taaagctgtc
     1321 gatttccaag gttggaaggt tttgtttgag ttctttatgg tagtgattga ccagttttcc
     1381 tttggctgaa atccaagtgt tgttttcata ctgccgggta ttgcccttgg tcagcaatcc
     1441 atagacacca gaatctgcga tgcagtgaat aatgccgaag cggaacagaa attggctatt
     1501 agcatgactg gggtcattat agacaaagcc tgtgaactgg attgtcttac cctcaaattc
     1561 atctggatag tcgtagatag cctccatgac ctccatatag ttttcattag tgatctgaat
     1621 actatcttgg gataagtatt tatccgccgc cgttcgcatt tccttttcat aggctgattt
     1681 tgaaaaataa gaactggtat ctggtttcaa atattggctt gtcgtccctt cgcttgtctg
     1741 aatggctaga tccgttcctt ccgatagagg gaaatgatag cctttagctg aaacagtctg
     1801 agaatccaag ctaacagttg ggaaagttaa gccgatgaca agtggaatag ccagaagaga
     1861 aatactcgtc atcttggcca atcggctgtt cagatgactg tgggttttga cttgcttcat
     1921 ccagatatac aattgaacga tagccaagat aaaagaaagc accatggaga tataggccag
     1981 ataggaatag tgcatgttga tgtactggtt tagtttgccc gacagatgga ggtaaatagt
     2041 cagttcaaaa tagccagcta aaactaaaaa tcgaatcata ggatgactcc aatcaagaga
     2101 gaatagacta agacgacaag agttacaatt gttatgaagt gactgataaa tcgtgctttc
     2161 aagtaatttt tcatcatgag aatatttttg atatccagca ttggaccaat gacgagaaag
     2221 gccagaactg gtgccaaacc gaaactcgag agaagagaag cacctataaa ggcatccgcc
     2281 tcactacaga gcgaaagaag aaaggctaaa atcatcaaga gcaggatggc aaaaagaggg
     2341 gtcgcactga tagaggtcag aatccgagtc ggaacgtaga cctgtattat agaagcaaag
     2401 aggcagccaa ataccaaata acgccccgta tcaaaaaatt catcaatggc ctgcacaaag
     2461 acttgaaaaa ctttttttgc agaactcaag taagaaaaat catgctcatg acaagccaga
     2521 cgattttctt tctgaatcgg ttcttgccag aaaaatccta gaaaaattcc tagtattaca
     2581 gccacaagaa tggaacccag agctcgtaat agggcgacat ggaaggagtt gccaaaggca
     2641 gaataggtcg caaaaagaac aatgggattg ataacaggtg ctgtcacaag aaaaggaacg
     2701 gccgtgtaac ttggaacctt tttttccaga aaacgattga tgatggggac gattccacat
     2761 tcacaagaag ggaaaagtat accgacaaag gtcccaaaaa agattctccc ccaacgattt
     2821 cgagggagaa aatgataaac cttgtcaggt gtgatataaa cttcaatcag ccctgagaca
     2881 atgcttccta tcagaacgaa gggaagggct tcaatgataa tggagagaaa aatggctcca
     2941 gtttgaagta cactagatgg gagttgttga aaagcagtca tctagttttt cttttctgct
     3001 ttggcttttt tctctttgtc atcagggaaa gtaacttgtt tcaaatcagg gagtttagca
     3061 aactcttcaa acatcttgtc caagtcgtct cgttttgtaa attttacagc cataggtgta
     3121 cctcgattct aaattctacc tctattatac tattattttg cggaaaaggg agttttttaa
     3181 tttgttttcg atagttgata ctcttcgaaa atctcttcaa accacgtcag cttcgcctta
     3241 ctgtagtacg gttactgact tcgtcagttc tatccacaac ctcaaaacag tattttgagc
     3301 agcctacggc tagcttccta gtttgctttt tgatttttat tgagtatgaa aaggattgga
     3361 gaaagccttc cagcttgtgg tataatagat ttatggataa aaaatatgaa aaaatctctc
     3421 aggatttggg agtgacgtta aagcaaattg ataccgttct aagtttgaca gctgaagggg
     3481 cgactattcc ctttatcgcg cgttatcgca aggacatgac tggtagtctg gatgaggtgg
     3541 cgattaaggc tattattgat ttggataaaa gtctgacaaa tctcaatgac cgtaaggaag
     3601 ctgtcttagc taagattcaa gaacaaggta agttgaccaa ggaattggaa gaagctatct
     3661 tagttgccga aaaattagca gacgttgaag aactctatct tccttataag gaaaagcgtc
     3721 gtaccaaggc aaccattgcc cgtgaagctg gactctttcc tcttgctcgc ttgattttgc
     3781 agaatatagt tgacttagag aaagaagctg aaaagttcgt ctgtgaagga tttgcgactg
     3841 gcaaggaagc cttgaccggt gcagttgata ttttggtcga agccttatcg gaagatgtga
     3901 ccttgcgttc tatgacttat caggaagtgc tgagacactc taaactcact tctcaagcca
     3961 aggatgaaag tcttgatgaa aagcaggttt ttcagattta ttatgatttt tcagagacag
     4021 ttggaactat gcaaggctat cgtaccttgg ctctcaatcg tggggagaaa cttggtgtct
     4081 tgaagatcgg ttttgaacat gcgacggacc gtattcttgc cttctttgct actcgtttca
     4141 aggtgaaaaa tgcttatatt gatgaagttg ttcagcaatc cgttaagaaa aaggtcttgc
     4201 ctgctattga gcgtcgtatt cggacagaat taactgagaa agctgaagag ggagctatcc
     4261 aacttttttc tgacaatctg cgcaatctcc tcttggttgc tccactgaaa gggcgcgtgg
     4321 ttcttgggtt tgacccagcc tttcgtacag gtgccaagct agctgtcgtg gatgcaacag
     4381 gaaaaatgct gacaactcag gttatttatc ctgttaaacc agcatcagct cgtcaaatcg
     4441 aagaagccaa gaaagattta gcagatttaa ttggtcaata cggtgtagag attattgcca
     4501 ttggaaatgg aacggccagt cgtgaaagtg aagcttttgt agcggaagtt ctgaaagatt
     4561 tccctgaagt cagctatgtt atcgttaatg aaagtggtgc ttctgtctat tctgccagcg
     4621 aacttgctcg tcaggagttt ccagacttga ccgttgaaaa acgctctgcc atttctatcg
     4681 cccgtcgttt gcaagatcct cttgcggaat tggtcaaaat cgatcctaag tcaattggtg
     4741 tcggtcaata ccaacacgat gtcagtcaga agaaactatc tgagagtctg gactttgttg
     4801 tcgatacagt ggttaaccaa gttggtgtca atgtcaatac agctagccca gctcttcttt
     4861 cacacgtagc tggactcaat aaaaccattt ctgaaaatat cgtcaaatac cgtgaggaag
     4921 agggaaaaat cacttcacgc tctcaaatca agaaagttcc tcgtttgggg gccaaggctt
     4981 ttgaacaggc tgctggtttc ctccgtatcc ctgaaagtag caatatcctt gataatacag
     5041 gagttcaccc agaaaattac gctgccgtta aggaactctt caaatgcttg gacattaagg
     5101 atctgaatga agaggcccaa agtaaactca agtcgctttc agtcaaggaa atggcgcaag
     5161 agttagacct tggtccagaa actcttaaag atatcattgc agatcttctc aaaccaggtc
     5221 gagatttccg tgattccttt gatgctccag ttctccgtca agatgtccta gatatcaagg
     5281 acttagtggt tggacaaaag ctagaaggag tggtgcgtaa tgttgttgat ttcggtgctt
     5341 ttgttgatat cggaattcat gaggacggtc tcatccatat ttcccatatg agtcgcaagt
     5401 ttatcaaaca ccctagtcaa gtggtggcag ttggagattt ggtgagcgtt tgggttaaga
     5461 aaattgatac tgaacgtgaa aaagttaatc tgtctctcct agctccaaat gaaactgact
     5521 gattatgtca agcaggtttc actagaagac ttcggcagac ctttcatcca tcatgtccag
     5581 tggaataggc gtctacgttc gacaggtggg cgatttttcc ccaaagatgg gcatctggat
     5641 tttaatccca aggtttatca gaaactgggt atggaggttt ttaggaaaat tgtccgacat
     5701 gaactctgtc attatcacct ttatttccaa gggaagggct atcaacacaa ggatcgggat
     5761 tttaaggaac tgttgaaagc agtggatgga ttaaggtttg taccatcctt gcccaatagt
     5821 aactctaaac cactcaagct ttatcgttgt caatcctgcc agcaaaggta tcagcgcaag
     5881 cgtaggattg ataccaaacg ctatcgctgt ggactttgtc gaggtaaatt gcttctgata
     5941 aatcagcctg aggactgatg aaagccgagc ctccccgtga tatactatgt ctaacctagc
     6001 agaaagagga aatatcatga acagtaaatt ttataaaatg agacgcaatc gcatgatttc
     6061 aggagttctg gcaggtctgt cagacaagtg gaattttgat gttaccctag ttcgtttctt
     6121 gtttgccatt tttacagttg caaactttgg acttggcgtg attatctata tcatcctagc
     6181 ttctatcatg cctaccaagg aagaaatcga agcagaaatg tacggaacag gcccacgtaa
     6241 acgcaaagaa gcccaagcca ttgacgacaa tgaaggctgg ttttggtgag gttctacaga
     6301 agatttggaa taagcaaaaa gctttaaatc aatagtctaa actgcttgat ttaaagcttt
     6361 tttactttta tactcaataa aaatcaaagt gcaaactagg aagccagcca caggctgtaa
     6421 tttagtcggc aatgtgaagt tgacatagtt tgcactttga tttttgaata gtattaataa
     6481 tatttcgcca gcttcatcca caaactatag tggattggaa ctagaatagg acacctctac
     6541 ttctaaaaca ttgttagaaa tcgatttgac tatcctgatc gatttgtcct gttcttattt
     6601 cattttatta taaaagcagt aatttaaact cagtttttta atctattttt gctgctgaga
     6661 caatacattt ttggaaaaat ggtggatgct gataacaact gataaccagc aaataacata
     6721 agcagtgccg gtaaattctg taccgataaa gtgtgtgtgt tcaactaaga gctgaccgat
     6781 gaatggagag agcagataga gaagtccgat gagtatatgt tctttgtgtt tcattaaagc
     6841 gcaccttctt tccttatagg ttgaacgact tgcaagtgtt aaattgaatg gtatgttatc
     6901 taggtaacct tactaaaatg ttagttcccg cttcctcctt ttcaaagtaa gccttttcac
     6961 acttatattt tacaacttta aaaataggaa atcaaatcat tttctaagta attttgctta
     7021 tatttcaccc aatcttacaa aaatgtaaga ttaaagtctc ttttgtaagg ttaggttatg
     7081 gcaagcgatc tttttttgat gtaaactaga ctcataaaga acaaaggagt aacatcatga
     7141 aacaaatctt acataagaaa actagaaaac caagtcacaa agaaattgag cgcgttcagc
     7201 tgggatgcac tatgatgcaa gctacatttc atttgatggg atattaagaa aggagattct
     7261 catgacactt ttagatgtaa aacacgttca aaaaatttat aaaacacgtt ttcagggcaa
     7321 ccaagtagaa gccctcaagg atattcactt taccgtcgaa aagggtgact acgttgccat
     7381 catgggtgag tctggttctg gtaaatcaac tcttctcaat attctagcta tgctggataa
     7441 accaagtcgt ggtcaggttt acttgaatgg aactgacacc gcaaccatta aaaattcaca
     7501 ggcctctagc ttccgtcgtg aaaagttggg ctttgtcttc caagacttta acttgctaga
     7561 tactctatct gtaaaggaca atatcttgct tccgcttgtc ttgtcaagaa gacctattac
     7621 agagatgatg aagaaattgg tggtgacagc tgagaatctg ggaataaacc aattacaaga
     7681 gaagtaccct tacgagattt ctggtggcca gaaacagcgt gtagcagtag cacgcgccat
     7741 catcactgaa cctgaaattc tccttgcgga cgaaccgaca ggagctcttg actccaagtc
     7801 atctgcagcc ttacttgatg tctttgatga aatcaatgag cgcggccaaa ccattctcat
     7861 ggtaacccac tcaacagcag cagctagcag agctaaacgt gtactcttta tcaaagacgg
     7921 cattctttac aatcaaatct accgtggaga gaagacagaa cgtcagatgt tccaagaaat
     7981 ctctgatacc ttgactgtca tggcaagcga ggtgaattag tatgtttcga ttaaccaata
     8041 agttagcggt atcgaacttg attaaaaacc gcaaactcta ctatcctttt gcgctggctg
     8101 ttctcttggc agtcactgtc acctatctct tttactctct aaccttcaat cctaagattg
     8161 cggaaatccg tggaggaaca accattcagg ctacacttgg atttggtatg tttgtcgtca
     8221 cccttgcgtc agccattatc gttctctatg ccaatagttt tgtcatgaag aaccgttcca
     8281 aggaactagg aatttatggc atgttgggct tggagaagcg tcatcttatc agtatgacct
     8341 ttaaggagtt agtggtattt gggattctaa ctgttggagc gggtatcggt attggagcct
     8401 tgtttgacaa gttaattttc gctttcctgc tcaaactaat gaaattgaag gttgagctgg
     8461 ttgctacctt ccagacgaaa gttgtcatta cagtgcttgt tgtcttcggt ttgattttcc
     8521 taggcctcat gttcctgaat gcccttcgaa tcgcccgtat gaatgccctc cagctctctc
     8581 gtgagaaagc tagtggagag aaaaaaggtc gcttccttcc tctccaaacc attcttggtt
     8641 ccataagttt aggaattggc tattatcttg cccttacggt aaaagatcct cttacagcct
     8701 taacaacctt cttcatagct gttttactgg ttatctttgg gacttatctc ttgtttaatg
     8761 cagggattac cgttttcctc caaatcttaa agaaaaataa gaaatactat taccaaccaa
     8821 ataacctcat atctgtttct aacttgattt tccgtatgaa gaaaaatgca gttggactag
     8881 ctaccatcgc tatcttgtca acaatggttt tggtaaccat gtcagcagcg acaagcattt
     8941 tcaattcctc agaaagcttt aaaaaagttc taaatcctca tgattttggg gtttcagggc
     9001 aaaatgttga aaaagaagac ttggacaaac tcttgagcca gtttgcaagt gacaaaggtt
     9061 ataagattaa agaaaaagaa gtatttcgtt acacttactt tgctgttgcg aatcaagaag
     9121 gaaccaagtt aactattttt gaaaaaggac aaaaccgtgt ccaaccaaaa acagttttca
     9181 tggtatttga ccaaaaagat tatgaaaata tgactggtca aaaactgtct ctatcaggaa
     9241 atgaggtcgg actctttgca aagaatgagg gagttaaaga acagaaagct ctaactctaa
     9301 atgatcatca attttctgta aaagaagaat ttactaaaga ttttattgtc aaccatgttc
     9361 caaatcagtt taatattttg actgctgatt acaattacct tgttgtacct gatttacaag
     9421 cctttttgga tcaattccca gattcggcta tctataatca gttttacggt ggtatgaatg
     9481 taaatgccag tgaagcagaa caactcaagg tcgctgagga gtatgaaaaa tacttacaaa
     9541 agtttaatgc tcaattaaac actgaaggta actatgtgta tggtagcact ctagcagatg
     9601 ctagtgctca gatgagtgcc ctctttggtg gtgtcttctt tatcggtatt ttcttatcca
     9661 ttatctttat ggtcggaacc gttctggtca tctactacaa acaaatttct gaaggatatg
     9721 aagaccgtga gcgctttatt atcttgcaga aagtcggttt agatcaaaag caaatcaagc
     9781 aaaccatcaa caaacaggtt ttaactgtat tcttccttcc tttgctcttt gccttcctac
     9841 atctagcctt tgcctaccat atgcttagtc tgattttaaa agtgattggt gtactggata
     9901 cgactatgat gttgattgtg accttgtcta tctgcgctat cttcctcatc gcctatgtgc
     9961 tgattttcat gattacttca agaagttatc gtaagattgt ccaaatgtaa aaaaagatac
    10021 ctcgacttca aaatcgaggt atttcttgtt ttctaaatgc tgaaaagttg tccgagcagg
    10081 aaggtaactc ccatagttaa gagaccaata gcaaggttcc gaatcatagc tgttttggtt
    10141 ggggcttttc caagtctagc acttgtgtag ccagtgagaa gaagggctac accgacgata
    10201 aggacggtag cagggatgcg gtaatcactt ggaaaaatgg tcactgacag cattggaggc
    10261 aaacttctaa ggaaaaaggc aacgaagcta gaaatggcag cgtgccaagg attggtaaat
    10321 tcttcatact caatcccata tttttcctct accagagcct tgagtggatt tttaagaaag
    10381 atcttattag tcaagagttg ggcagaagtt ttgaattctc cattttggat ataagaagca
    10441 tagagggatt ttttggctag ttccctatct tggtctagca agagtttttc tcgcgaaacg
    10501 gcagcttcct cggtatcttt tggagttgaa acggatacat attctccacc agcaatggaa
    10561 ataattccat cgttagcatc aagaacaccc gcacgcagga tatttaaacg acctgcaaaa
    10621 tttgaatcaa tttcgtgatt tgtttctgac gctaaatttc aagttcaagt tagccatcca
    10681 gaagtcttct ctgggtgact tgtagtccaa gcatttttta ggatagttgt taatccactt
    10741 ttcgatgaat gcgacttctt tgggagtcat tttcttggtt cccttaggta accatctacg
    10801 aatgagcctg ttgtgattct cattagttcc cctttcccaa gaggcatagg ggtgcgcata
    10861 atagatgtgc tcctcagaaa atacatcaaa caagcgattg aattccgttc cattatctgc
    10921 cgtgatggaa agaatcttgt gttgttttaa gatgagtttt agagcctgat tgaccacctc
    10981 agcactttta tttggaatca atcggatgat ctgatgtcta ctctttcgat ccgtcaagac
    11041 aagcaagcag tagtttttcg ctctcgtaag tagaaccgta tcaatctcat aatgcccatt
    11101 ctccaagcga agattgatag cttcagaccg ctgttcgatg gattgaccag caggtttaaa
    11161 gttggtgcta gcctgtttct tatcaggcga aaatttttgg ttatgatagt ggagaatctt
    11221 ttcctttagt tccttggtca agcttgattt cttgaccgag cgcttgcgat tgttttcata
    11281 agactgttga gcatagtcgg cagaataaac ctctttgaag cgcccttttc caagacattg
    11341 tcggactgtc ccacgcttga tttcagtgtg gatagtttga ggagcttttc caagtagaga
    11401 ggcaatttct ctatttgatt tcccttcttt tttccatctt tcgattaagc gacggctatc
    11461 gattgtcaaa tgtttacctt ttgtagtata attgtcttgc atttctgtgc ctttcttgtg
    11521 tttgtggttg aacaacaagt ataacacaga ggtgttttct tatgcctaca agagttttca
    11581 ttatttccat tttctttcgg atttcactct attctgaaaa acttgtgtat atttactgaa
    11641 gctagcaagt cttacctgta aatttaatga aagcaacaca aaatcctaga ggggaatctc
    11701 ggattaatgt caatttaaaa agatagagag tttttagttt aaataaattg ttttaaaata
    11761 tcaacaacat cacttctttt cttaacctga taagccttga ttcctaattt tggagctacg
    11821 attatattgt cctcaatatc gtctagaaag acacaatttc taggttataa ctggtattta
    11881 tcgatagtta ctcatataca tcagtacacc tccatactta tgtgcgagcc tctctttgta
    11941 ttatacctcc atactcacct tacagattct tttggtaata atatctttgc ctaatgtaga
    12001 gatagtcttg caaagaaaaa acttccttgt agccatgttt ctgataaaag tccggtgcct
    12061 ggaactgata agtattgaca aaggcaaaac aacaatttcg attcttagct tcactttctg
    12121 cctgttgcaa tagttttgaa ccgattcctt gccctcgcag ttcctctttt acaaacaaat
    12181 actcgatttc tagccaattt ccaaaagtct ctgctatcaa acccgcaagg agattgccct
    12241 tttcatcttc gacataaaga ttaagtggct cactttcagc ctcttccctt tttgaacggt
    12301 tataaacacg aatcagattc cctatttctt gcgatttatg tgattcctta ttttccaatc
    12361 taaagtataa tgaaatgaaa taaaacatgc gcaaatcgat taaggaattt aatctaattt
    12421 ctaataatgt cttagaaatc aaagtttact attttaactt caatgcacta tacatctaat
    12481 actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca aaacactgtt
    12541 ttgaggttgt agatgaaact gacaaagtca gtaaccatac atacggcaag gcgacgctga
    12601 cgtggtttga agagattttc gaagaatagc aaaatggaaa aaggagtgag tgaagcacat
    12661 cgcctcccca ctcctttttc tgtttttagg ctgttttttc aaccttcaag atttttacat
    12721 catagctacc aacaggcgtt tcaatggttg ctgtgtcacc tgttttcttg ccaatcaagg
    12781 cctgcccaat tgggctttca tttgaaacct tacctgcaaa ggcatccgca ccagctgaac
    12841 ctacgataat ataaacttct tcttcgtcct caccaatttc ttggatggtg actgttttac
    12901 caatcgctac ttcgtcctgg gcaactgcgt cgctattgac gatttcagca tagcggattt
    12961 ttgtttctaa gctagagatt tgtccttcga caaaggcttg ttcatcctta gctgcttcgt
    13021 actcactgtt ttctgaaagg tcaccgtatg aacgggcaat cttaatgcgt tctaccactt
    13081 ctggtcgacg aaccaatttc aattcttcta attctttttc aagtttttcc ttttcctcaa
    13141 gggtcatagg atatgttttt tctgccattt ttctcaactt tcttctgata atattttcta
    13201 aagaaaatta tgtgacgtat cacataattt tagtttgttt agtttaattt gctgttgaca
    13261 tgttcagcga cattgcggtc gtggtcttct tgattgttag catagtagac cttgccttct
    13321 gtgacatctg ctacaaagta gaggttatcg ctcttagttt gattgatgct tgactcaatc
    13381 gcatccagac ttggactatc gactggacca ggcatgagac ctacattttt ataaacatta
    13441 taaggtgaat caatgttggt atcaatcgca acatcctcag ctagactgat attttgcccc
    13501 agttttcctt gggcatacaa gattgcaata ttactttgaa gtggcatatc acgatttaaa
    13561 cgattgtaga atacacctgc aatgagctta cgatcttctg tcttggcacc ttctttttcg
    13621 accaaggaag caatggtcaa caactcattg acagtcaaat ttttagattt gatagtacta
    13681 tagtaaggag ataggttctt atccatagca gctaacatct catcaatcaa gctctcaata
    13741 gttgtgcttt ccttgataga gtatgtagct gggaaaaggt atccttccaa acgataacgc
    13801 gcaccgctgt cttttacagg caaactttcc agtaaagtag gatatttcgc tactgcttga
    13861 ctgataaacg tctcatcttg aactttagcc aagaaagcct ccgctgtcaa agactctttg
    13921 aagtcacctt gcaattgacc cacagcttga gcaatcttat ccaaggtata accttctgga
    13981 attgtcaaag tcgcaagtac aggttcttgc ggttcatctg ttccaccttt ttgcaactct
    14041 ttgagtaagt cttctgtact catactcttt tgcaaattgt agtaacctgc tttcaagtcg
    14101 gtataatttt tatacttggc ataaaaacta aaaatcagac catgctttac caaaccagct
    14161 ttttcaagcg tcgtaccgat ttcttgaacg tttgaacctt ctggaattcc aaccgtcaca
    14221 tatttcttag aattagcatc gataggtaac aaagaatcta gcacgtactg gtaaccgaag
    14281 taaccacctg ctgagagcaa ggcaaggaat acaaggaatg aaatcacaaa agctttcaca
    14341 tgtgacttct tctctttctt aggcttaacg ggttttgctc cttctctacg gctacgacgt
    14401 gatgtatcac tgatgatatc cactatttct gtagcaggcg taggtgtttc tggacctgct
    14461 tgttctgctt ttttctctgc cgtcgtatag gaaacagcta cccttgttgg ggtttcattg
    14521 tattctcttt caagtttctt aggtctaaca ggacctggac ctggtcttga tccactttct
    14581 tccgctggag aagaaggtac atcttgactt ggatgacttg gaacaccagg agtttctctt
    14641 tgactctcat ctgctggaga agctggtaca ccttgacttg ggtgagtagg cacggtagga
    14701 gcttttctca taatctcctc taccgttgac aaggaatcag ccatgagttc ttcagctgaa
    14761 ggttcatttg caggagtgcg aactactgcc tcatcttctt tcagaacttc atcatagcct
    14821 tttacttttt ctaaatctct cagaatctgc tctttaaagc ttaatttctc ttcttctctt
    14881 gacttttcac tcaaaagttt ttcctccttg ttgataatcc ataatattat atcttaaaag
    14941 tccaaaaaaa gcaatctatg atacttttcc taacttattt tttcatttcc cagaccatat
    15001 cataccatgt ttcccctgca aaggttgact gggactggcc ttcgttgaca aatccatgtt
    15061 tttcataata ggcgatgaga tagtcatgac aggttagatt aatgccatct ctctcatctt
    15121 caagagcaac ttctttcaag gctgtcagca atttctgacc cagtccgagt ccctgtgcct
    15181 ccttggcaat agacagacag gtcacagaga tataaccacc aggctcatga ctatagtctt
    15241 ttatttcttc tgtaaaagac tggtcttgca gatggcggtg caggccaact ggtccttcga
    15301 tataacccat gattcttcct tctttttcag caaccagaaa agaggtctga atttctctca
    15361 aatgtgcttc aaagacagaa ggaggaatgg cttcttcgac cgaaaaattt tcaaattcaa
    15421 gttcaacaat ccgatccaaa tcttctaatc ttgcttgtct gattttcatt gttcctccag
    15481 ataaaaggga ttaaaccaaa tcatactata gccctggcta gttgcataga gcaaagtttc
    15541 ttcttcatca acaaaaccgt tcatttcaaa ataggaaagc agctcatcag gactctccaa
    15601 acgaatccct ttgtaatcca gctcaactgc cacctctttc aaggctgcaa gaagaagtgt
    15661 tcccaggccc tgtctctgat ggtcagactc gatgactaaa gaatgtactt ttagacattg
    15721 cggattgtct gactggggac ttgataaaat atagcctaaa agttgatttt catccctagc
    15781 tagaagaaag gtatccgcac acttacggat actttcttct aaaatatggg aaagttgctg
    15841 cttttcagct ggaaaagacg aggtctgaag tgcccctatc tcaggcaaat caaacttgct
    15901 tgcctgaatg atcttaattg gaatttccat gggaacatcc tattgaacat tgcttgtcaa
    15961 gttagacaag agacgctcaa atgagtattc ataggtttgg atgtctcctg ctcccataaa
    16021 gacataaaca gcattgtcat ggtctaggag tggagaaaca ttttcaacag taatcacttg
    16081 gtgttttttg ttgattttat tggctaggtc ttctacctta acgtcaccat gatctacttc
    16141 acgagccgag ccataaattt gcgctagata aacagcatct gcttggttta aagcatgggc
    16201 aaagtcgtcc aacagggcaa tggttcttgt aaaggtatgc ggttgaaaga ctgctacaat
    16261 ttccttgctt gggtatttct gacgagccgc atccaaggtc gcaataattt ctgttggatg
    16321 atgggcaaag tcatcaataa tcactgtatc attgacaatt ttctcagtga aacgacgttt
    16381 aacaccggca aatgttttca agtgctcacg caccaagttc aaatcaaatc ctgctgtgta
    16441 aagaagacca ataacggctg tcgcattcat gatattgtga cgaccaaagg ttggaatgtg
    16501 gaattgcccc aagttttgtc cacggaaatg aacggtgaag gttgaaccag ttgttgaacg
    16561 aagaagatca ctagctacaa agtcattgcc ttcagcttca aaaccataat aataaattgg
    16621 tgcatcagac gtaatcttac gcaattcagc atcttcacca tagacaaaaa gacccttggt
    16681 aatttgtttg gcatagtcgt taaaggcatt gaaaacatcc tcgagacttg tgaaataatc
    16741 tggatggtca aagtcaatgt tggtgataat agagtattct gggtggtaag gcatgaagtg
    16801 acgctcatat tcgtcagatt caaagacaaa atatttggca ttagccgaac cacgacctgt
    16861 cccatctcca atcaagaagc tggtatctgt aatgtgagac aagacatgag acaacatacc
    16921 tgtcgttgaa gtttttccat gtgctcctgc tactcccatg ctaacaaagt cacgcataaa
    16981 gctacctaga aactcatggt aacgtttgta gctgatacca ttttggtccg cataggcaat
    17041 ttcgacgttg ttatctggac gaaaggcatt tccagcgata atttccatat caccgtctag
    17101 attcttttca tcaaaaggaa gaatggtaat tcctgcctgc tcaagaccgc gttgggtaaa
    17161 gtagtacttt tcaacatctg atccctgaac cttgtgcccc atctggtgca acatcaaggc
    17221 caaggcactc atccctgatc ccttaattcc gataaaatga tatgtctttg acatgttttc
    17281 tcccctattc tgtcattctg gtcagattca actcttgggc aacccgacgt tcttgttctg
    17341 tttgtttact ttttttattg tagatttggc tcttctttag aaaatcataa ttgtttttct
    17401 ttggagcagg tgctgacact tcttcattct tggtagggat agaatgaact tcttccgcca
    17461 agatataatg agactgggtc aatttttggc tatatttgac aaattcacca ggattttcct
    17521 tttggaaagg agttgtcggt tgattgccct gtctaactag actgggctga gaatgacgtc
    17581 tcgcaaggct gaaatcctga gttaggtagt tagcagagcg tttctttttc aagtccgcac
    17641 gcgcttcttc acgcgccacc tccgcatagc tctttccttc ttttttaacc cctaaaggag
    17701 cctttttagg tttttcgact tgcttttcaa tcggttttac tggtttttct tcagtaatag
    17761 gagcccattc taaataattt ttatctcgat actcaccctt gatattactg atcagatcag
    17821 actcatcata gagattcatg actggcattt cagtcaacat gacctcgtca tctgacacca
    17881 atggaaatcg ttcttgtttc attttctatt tcctttcaac acttcattat agcgtattgt
    17941 cttgattttt caagtgctgg cttcagaaat tcccaaaatt tctctaattt ctgctagggt
    18001 cagactacca cgtgactctg tgccatccaa tacttgtgac accagatgtt tcttttgttc
    18061 ttggagttcc tgaatttttt cttcaatggt tcccttggtc accaagcgat agacctcaac
    18121 cgtttcttcc tgacccatcc gatgggcacg gccaatggct tgcgcttcca ccgcaggatt
    18181 ccaccaaagg tcaaccaaga tcactgtatc tgcacctgtc aggttcagac cgaccccacc
    18241 agccttgagg gaaatcagaa aggcatctct ttctccttgg ttaaaggcct tggtcatgtc
    18301 ttgtctttcc ttggctgggg ttgaacccgt aattttaaag gatgtcaggc ccaagtctgg
    18361 cagttcttgt tcaatttttt ccaacattcc cttgaactga gagaaaatca agacacggtg
    18421 tccgccgtct gccacctgta ccagtaggtc tcggagacta tctagtttgc cgctggctcc
    18481 ctgataatct tccataaaca gggcaggagt gtcacatatt tgacgcaagc gcatcaaacc
    18541 agataaaatt tccacacgac ttcgctgaaa ttcctgttct gacacttgag ccagatggtc
    18601 tcgcatctgt tgtaactggg caaggtaaat agccttttgc tggtcttcca gttcattttt
    18661 ataaaccacc tcaatcaagt ctggcaattc agtcagaact tcttctttct tgcgtcgcat
    18721 cacgaaaggc ttgataaact gagccactcg ctctgctggc aatttcataa attctttctt
    18781 gcttggcaaa agtccgggca tgacgatttg gaaaatagac cacaactcac ccagatggtt
    18841 ttcaatcgga gttcctgaca aggcaaagac cgacggcacc acaaattgtc tcaaggtctg
    18901 ggcaatcttg gtctgggcat ttttcatgac ctgagcctca tctaagaaaa ggaagtcaaa
    18961 ggccatcccc tgataaaact cactgtcctg acggaaggtg gcatagctag tcacatagat
    19021 ttgatggctc tcggcaagaa tctcctcacg acttgctttc aaaccatgaa caacagccac
    19081 atccaactgt ggggcaaatt tctgaaactc atctgcccag ttgtaaatca aacctgacgg
    19141 agcgagaatc aaaacccgac tttcttttgt cacttgacta gtcaaaaaag caatggtctg
    19201 aagggttttc ccaagtccca tatcatcagc caaaatccca ccaaaaccat aatgatggag
    19261 catctgcaac cagccaattc ccttttcctg ataatctcgc aagtcagcct gaacctgagt
    19321 tgcctgtcga ggaaagtcct ctggatgtgt caaatcctgg gccagattct ggaattcttg
    19381 tgaaaaagaa acacggtctc gcccttcaaa gagatgagct aaactgtagg ccaaggattt
    19441 ccgagcctgc aaggtcccat cttttaattc aaattgcccc agttcctgta gattttggcg
    19501 aattttcttg gtttcttcat cgaaaaagta aacttgatta gacgaatcaa tataaaaatc
    19561 ctgattggca accaaggcct gcatggcttg gtcgatttcc tcctgggcaa tatcttgaaa
    19621 atcaaactgg atttccaaga gacctccctt ggaggcaatc tgcacctgag gactcgctag
    19681 gctataaagc tcttctagtt tatctgatag gtcaacatgc ccgagttttt caaagactgg
    19741 aatgatatca tgaaaaaaat gatagacaga ctccgctttt aaggcctgac gccaagattg
    19801 aaaatcggct tcaaagcccg cagccaaaca gacttggaaa attctttctt ctaagtctgc
    19861 gtcacttgaa aatggtaatt cttctagctc ttgtcggcta gatacctgtc tatttccata
    19921 atcaaactga atttccaaac gaatccgatt atcttcttcc ctgtcaaagt aaaaagaggg
    19981 cgcaaaagtt ttgatttgta gacgttctgg agctgaaacg gtgcccatct ggataaaaag
    20041 agtcagacag gaggccaatt tgtctcgatc actgctatca aattgcaggt atttctttcc
    20101 ttgttgaccc acaggtaacg ctttaatttc cttgagaaga cgcatctgct ggtctgttaa
    20161 aaaataaacc tgacctttat ggaaaagtac tgctccctga taaaagacat tgaccctagg
    20221 actctcactg atttccattt caaaatagtc cgagtattct gttactgtaa aggcaaatag
    20281 attggcatca gcatgcatat cctgaaaaag cagggtttgg tagctatcca cttgatggtc
    20341 aaattgaaaa tggggcaagg ccatcagtaa attcacaccc tgctcaaaaa aggtcagagg
    20401 gaaaaagagg tgccgacctt ggttttggaa aaagaggtct gaaaccagcc cttcctccgt
    20461 tagtccgtgc aagaaagtca aaagttcttg gctggcatca tcaaaggctt cccaagaaag
    20521 agactcctca taaatcttgc caatcatata cgactttctc tgctcgacaa tccttaaaaa
    20581 aagtggaata tcccgaatga catagtattt ttggctattg atttggccga ttctcagagt
    20641 ccacaagata tgattggttc ctgcttccac ctgacccaca gctgataact cataggcgca
    20701 ttctgatttt ggagataaaa ttcgatccaa aaacttgcca cccaaggtca ccttggtttc
    20761 aacggcctct ttttcttcat gaccttcttc cagactccac aagatttcct gaccacgctc
    20821 atcatttttc agaaaatgct ctagcgctgc caaatgcaca cagtaacccc tcttttgaaa
    20881 aaaatcacag gcacaaaaaa ccaaatcatc ctctaaacta tagcgcagtt cttcttctgc
    20941 aacgcgagcg tagagccgat tgttcttttc cttgatgata tcaaccttac cagtttcata
    21001 aagggcaaca ccttcgatac gaactttccc cggaatcaat ttagccatat tttcaccttt
    21061 accttatctt tttattatac catattttcg cctatgaaaa tagccttcta ggaagacttt
    21121 tctcctagaa ggctggattt ttaacgtttg gcaaaagtag ccacaatccg ctgacagact
    21181 tcttgcaaca gagatttggg catagctata ttgatgcggg catggagact tccttcctct
    21241 ccaaaatcca aaccacggtt gaggataacc ttggcttcat ttctcaacaa ctcttgcaat
    21301 gtttcatcag tcaggtcata agctgaaaag tcaagccaaa tcaagtaggt accttgcggt
    21361 ttcatgacct tgattttagt ctcttttcca aatagatcca tcacataatt gatgtggtct
    21421 tcaaagactt gcttgagttc ctctagccaa tctttaccgt atcgataggc agcttctgtc
    21481 gccaaataac ccaagcctga aatttcatgc tgattattgg ccaacaggcg tttctggaaa
    21541 gccagtctca acttaggatt ttcaatgact gcataggaat tttttgttcc agcaatatta
    21601 aatgttttag tggcactgct caagacgata gcaaaatttt tgaaggcagg attgatggta
    21661 ttgaaagact ggtgtttgtg accaaagagg gtcaaatctt ggtgaatctc atccgaaact
    21721 aacaaaacac cgtgtttttg gcagagttgg ccaatcttct ccaacacttc tttttcccaa
    21781 acacgtccac caggattgtg agggttgcaa agaacataga gtttaacctc ctcttccacc
    21841 aaatcctttt caagttggtc aaagtcaatc tcaaacagac tatccttttc cactaaggaa
    21901 ttagtaatca atctacgatt attcaacttg acactgcgag caaagggtgg gtagacaggc
    21961 gtgttaatta aaaccgcctc gccttctttt gtaaaggttt gaatagctgt tgagatggct
    22021 ggtaccacac cctcgataaa gacaagagcc tctttgtcaa agttgtaacc gtattgtgta
    22081 gcttcccact tttgaacttc cttaattaag tcttcactgg cataggtata accataaacc
    22141 agttggtctg cgtaagtttg cacggcttgg cggatttcag gcaagaccac aaagtccata
    22201 tccgctatcc aagctggtag aacttcacta tccgtttctg tttctttcca tttataggta
    22261 tggtgcccta aacggttggg caggcttgta aaatcatatt ttcccatctt tgtcttatcc
    22321 ttctaaggct tggcgcaaat ctgcaatcaa atctctagca tcctcaatcc caatagacaa
    22381 acgcaagagg tcatctgtca aaccataaga atggcgtact tctgctggaa tatcagcatg
    22441 agtttgagtc gttggataag taataagact ttccactccg cccaaacttt ccgcaaaaga
    22501 gaagaccttg agactgttca aaatatgagg aatgcgtgtt tcatcggcta ctttaaagga
    22561 aatcatgcct ccacgaccag tgtagagaac ttccttaact gctggagaat ccttcaaaaa
    22621 ggcaaccact tcttgggcgt tagctgttga gcgctccata cgaagagata aggtcttgag
    22681 accacgaagc aactggtagc tgtcaaatgg agacaagact gcccctgttg tattgagatt
    22741 gtaaaaaagc ttctcgtata gttctaaact attggtcaca accactccag ccaagacatc
    22801 attgtggcct gctagatact tggttgctga atggagaacg atatctgctc catcttcaat
    22861 cggacgttgg tagatagggc tatagaaggt attgtccacc accactttgg cacccttagc
    22921 atgagccaat tttgctagtt tttcgatatc aaattccaac atcaagggat tggttggggt
    22981 ttcgatatag agaacatcca catccttttc taactcggca atcaactctt cttctgtatt
    23041 ggcataggta aaatggaaat gaccttcctg ctccacttgg ttaaaccagc gaaaagaacc
    23101 accgtaaaga tcacgcactg ccaagacctt acttcctact ggaaagacgc taaaggccag
    23161 tacaatagct gacatccctg agctagtcgc tagggcatag tctgctgact caatagccgc
    23221 caagacttcc tcagccttac tacgagttgg atttttagtg cgcgtatagt caaacccagt
    23281 agatcgacca aactctggat gctgataggt cgttgaaaaa tgaagtggtg tcaccaaagc
    23341 acctgttgcc tcatctgact taatacccgc ctgggccaaa attgtgttaa tgtgtaattc
    23401 cttgctcata caatacctcc aaatctatag taactattgt accacttatt ttcatcaact
    23461 cattttcttc ttttcaagag ctagttatag tttcaaacta tataaaaagg gagtttttcc
    23521 tgctcccttt aatagactat aaaatggtga atctcaaaag acaccttcac tctatcattt
    23581 gctcctgcac aaaacgagca taacgctcat gattttccag tagttcctta tgagttcctg
    23641 agccagtgat tttcccctcc tctaagaaga aaatacaatc cacatctttt accgttgaca
    23701 aactatgcgc tataatcaca accgtcttct cctttaatac agaatagagg ctactgataa
    23761 tcgcatactc agaatccgca tcaagattag cagtggcttc atcaaatata agaatttcag
    23821 catcttttaa gtaggttcta gctatttgaa gtctttggct ttgcccccct gacaagaatc
    23881 gtccgcgttc accaacttca gtatctagtc cctctttcat ggagcgaatc tcatcaccta
    23941 gtgatactaa gtctagcact ttcatcaatt catcatcagt tactaagcga ttcaaaccga
    24001 gacaaagatt gtcacgaata ctgccagata agactgcatt attttgtgaa acccaagcga
    24061 ttttacttct ccattctttt aagttaaaat catatatact tgattgctcc attagaatat
    24121 ctcctgaaag cggtttataa aaccgctcta acaaacgcac aatcgttgat tttcctgatc
    24181 cagatggtcc aacaaaagca attttttgcc ccttgaaaat tgaacaagta atatccttta
    24241 agacaggtcg attttcatca taaccaaaat agacatggtt aaaattcaac ccttgtcctg
    24301 ataccgattt tcctccctca aatttttctt taggaactgc aagcaagttc tccagtgcaa
    24361 ctgaagatcc cttgctacta gaataaatag ttacaaaatt agctatatta ctaataggat
    24421 taagtaattg aaagaggtaa atcaaaaacg aaaccaaggt tcccacagat atatatcctg
    24481 cgctgacccg ataaccccca taggttagca tcacaactat agtcgcaaag ataaataaga
    24541 gagcaaacgg ggtctcaaaa gaagtaaccc tatctgattt cagtgaattg ttttgtaccc
    24601 tttcaataca attatccaaa acatcctgta cacttttctc tgcttggtta gtcttaatta
    24661 attcatgttc ttgaatcttt tcagtcaatt gccctgttaa aaatccgatt tcctcctgta
    24721 aacgacgact atacttttca ctgatattgg aaaggggcaa gataataaac atcatacaag
    24781 gaagagtgat gaataaaagt agagaaagat tccaatcaag actaaataag actacaatgg
    24841 aaccaagtac cataactaaa ctcagaataa tatttgggaa agtcgtaatt aaaaactcac
    24901 gaatgacact catgtcattg acaatggcag aagtcaactc cccactttgg ctcttatcaa
    24961 agaaggattt ctctgcataa atcaacccct ctatcacttt tttcctgatt tttgctatct
    25021 tttttcaccc gattgactaa acagatagta accaatagaa gaaaacaagg cttgaccaat
    25081 aaaaatcaaa aacgattgaa atactttgga acctatattt tcaatagaac tcccatctat
    25141 taaacccttt aagataaggg gaagcaacaa agcaagtaga ctagacagaa caagtaagaa
    25201 actcca
//
LOCUS       DAVHIU010000019        24659 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVHIU010000019 DAVHIU010000000
VERSION     DAVHIU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.019"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1291..3212
                     /locus_tag="VVN06_000643"
                     /pseudo
     CDS             1291..3212
                     /locus_tag="VVN06_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            3193..3396
                     /locus_tag="VVN06_000644"
     CDS             3193..3396
                     /locus_tag="VVN06_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562240.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMIASNFLSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            3399..3749
                     /locus_tag="VVN06_000645"
     CDS             3399..3749
                     /locus_tag="VVN06_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562241.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            3758..4057
                     /locus_tag="VVN06_000646"
     CDS             3758..4057
                     /locus_tag="VVN06_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562242.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            4061..4393
                     /locus_tag="VVN06_000647"
     CDS             4061..4393
                     /locus_tag="VVN06_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU5562243.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRVVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKR
                     MNDAWKGR"
     gene            4397..5353
                     /gene="lytA"
                     /locus_tag="VVN06_000648"
     CDS             4397..5353
                     /gene="lytA"
                     /locus_tag="VVN06_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU5562244.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGSMADKPEFTVEPDGLITVK"
     gene            complement(5605..6573)
                     /locus_tag="VVN06_000649"
     CDS             complement(5605..6573)
                     /locus_tag="VVN06_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5562245.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLNLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            6709..7566
                     /locus_tag="VVN06_000650"
     CDS             6709..7566
                     /locus_tag="VVN06_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU5562246.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            7553..8332
                     /locus_tag="VVN06_000651"
     CDS             7553..8332
                     /locus_tag="VVN06_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU5562247.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            8356..9708
                     /locus_tag="VVN06_000652"
     CDS             8356..9708
                     /locus_tag="VVN06_000652"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5562248.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            9782..10156
                     /locus_tag="VVN06_000653"
     CDS             9782..10156
                     /locus_tag="VVN06_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU5562249.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            10158..11006
                     /locus_tag="VVN06_000654"
     CDS             10158..11006
                     /locus_tag="VVN06_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5562250.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            11283..12050
                     /locus_tag="VVN06_000655"
     CDS             11283..12050
                     /locus_tag="VVN06_000655"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU5562251.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            12062..13246
                     /locus_tag="VVN06_000656"
     CDS             12062..13246
                     /locus_tag="VVN06_000656"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5562252.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSLLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            13243..15114
                     /locus_tag="VVN06_000657"
     CDS             13243..15114
                     /locus_tag="VVN06_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5562253.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(15257..16441)
                     /gene="mntE"
                     /locus_tag="VVN06_000658"
     CDS             complement(15257..16441)
                     /gene="mntE"
                     /locus_tag="VVN06_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU5562254.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            16745..19441
                     /locus_tag="VVN06_000659"
     CDS             16745..19441
                     /locus_tag="VVN06_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU5562255.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            19454..20245
                     /gene="yghU"
                     /locus_tag="VVN06_000660"
     CDS             19454..20245
                     /gene="yghU"
                     /locus_tag="VVN06_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU5562256.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            20361..20771
                     /locus_tag="VVN06_000661"
     CDS             20361..20771
                     /locus_tag="VVN06_000661"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5562257.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            20783..22378
                     /locus_tag="VVN06_000662"
     CDS             20783..22378
                     /locus_tag="VVN06_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562258.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            22444..22608
                     /locus_tag="VVN06_000663"
     CDS             22444..22608
                     /locus_tag="VVN06_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU5562259.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            22687..23415
                     /gene="yaaA"
                     /locus_tag="VVN06_000664"
     CDS             22687..23415
                     /gene="yaaA"
                     /locus_tag="VVN06_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU5562260.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(23466..>24659)
                     /locus_tag="VVN06_000665"
     CDS             complement(23466..>24659)
                     /locus_tag="VVN06_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562261.1"
                     /translation="NEGLPIYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPS
                     EIRGNEIDIRYFFAQYFSERYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRM
                     YKLMIAISIHRVKNGHFIDLPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTI
                     AQIFISFLQNDIFLDPQEFFNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELI
                     WHLHNTAFFERQEIFSTPILFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDH
                     PEQLEHLMYTILTHAENLSTQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFT
                     FDIWDELKTSPEILNQTDYDIIVSNFYIPGITKKFICRNHLSIINLVNHLNTLSNEIH
                     LSNTL"
BASE COUNT         7406 a         4623 c         5726 g         6904 t
ORIGIN      
        1 aatcgtctga ctgaaggcct tagaaccaaa gtggatatca gcgcactcaa cgtgactgct
       61 gaaaatatcc ggcaatctgt gaagagtctt gagacagaca cgcagaacaa gctaaatcag
      121 aagttgagtc aggctgaatt tgaggtgcga gccggctcta tccgtcagga aatcctgaac
      181 gcaaccaagg ataaagcaga taagacttta gttgtatctg aagctgggaa attgcgtgaa
      241 gaattttcaa aaatgaaggt gggaggacgg aatctatgga taaaatccaa gacggttgga
      301 gctgtaattg aaaaattacc tgaaaaccac gtcacaggtc aaaaagaatg ctataggcta
      361 gagaacaact ctactttaac gttcaacctt gaaccagatt tcagctcaag gttgtaccaa
      421 aaagttactt ttagcgcttg gatcaagtac gaaaatgtag tccaaggtcg aaatttttgg
      481 aatgtattta attgcttcaa acattatctt tttagaaaaa atagtgagac cggagtacag
      541 agtggtccag attatgctac gcttggtatg tataaaggtt cggcagattg gaaatatatt
      601 acattcactt atgactactc tgaaaaaaca aattttgatc aattgaagac atcattgcga
      661 ttcaatcttg aaggtgctac aagcggtaca gcttgggtaa caggaatcaa ggttgaaatc
      721 ggtagtgtgg cgacggactg gagtcctgcg cctgaggacg ctgatggtct cattactgaa
      781 gctaaggcta cctttgagcg aacagctcag ggcttgcgaa ccgacttatc agctattcag
      841 gaatatgtaa ataaagacgg tcagcgacag gaagccttac gcacttacag tcgtgaggag
      901 agcgcgagac aagcgacagc agtccgtgag ctggtcaatc gtgatttcgt tggtaaggct
      961 acttatcaag aagatgttaa gggtatcaat cagaggattg aagctgttaa aactagtgcg
     1021 aataaagaca tcgctagtca aatcgctagc tatcgtcaat ctgtagatgg taagttcacg
     1081 gatatttcaa gtcagataac tacttataag caagatgtgg gcggtcaaat cagtggtcta
     1141 tcaaatagac ttacaagcag tgagcaagga accactactc agatttcaaa tatttcaaat
     1201 cggataaaca gtaataagca aggcacagat aatcagattt caaatttaaa gactcaggtc
     1261 gctacaaaca aggataatgc tgaacgacaa atgggtagaa tatctgatca ggtttctgca
     1321 aacaaagcga atgctgatag tcaatttgcg aatgtgacca atcaactagc gcgaaaagta
     1381 gagactactg acttccagcg tgttaaggaa accagtaaac tttacgagcg gattttgggc
     1441 aatactgaaa atggaattgc ggataaggtt gctcgcatgg ctctgaccaa tcaactgttt
     1501 caggttgagg tggctaaaaa tgccagcaat ggtcaaaatt tgttgaaagg cacaaaagac
     1561 ttctcagggg gttggaaaaa caagggtgca aattggaaaa agcacgctga aaaatacaaa
     1621 ggtgttgatg tcctatttaa aaataattcg tggaatggcg ttggacaaga gattgatgca
     1681 aaaattggtg aagtctatac attcagctta tggatgaaga gcgactggaa gaatgataca
     1741 gtaaatttct atgtaaatag aaatggatct gttgagaaag gttggggtgt tccatctgaa
     1801 acatcggtcg ctataacaag tgaatggaaa cgttactcat ttaccttcaa aattactgtg
     1861 gacggcttca tctttcctcg tgtagaacga cttaatcaaa atacaaatct atatattgca
     1921 ggtcttaaac ttgaaaaagg atcgtatgca acaccgtaca ccgaagctcc tgaagacacg
     1981 gacgaagcga ttcgctctgt tcaaagccaa ctaactggct catgggtagt tcaaaacatc
     2041 aactcggctg gagatatcat ctctggaatc aatcttggcg ccaatggaca taaccgcttc
     2101 gttgggaaat tgacccacat cactggagag accctgattg acagagcagt catcaagtct
     2161 gccatggttg ataagctcaa aacggccaat tttgaagctg gttcggtcac gactacgata
     2221 ttagaagctg aagcggtaac tgctgagaag ttgaaagttg acaatgcgct tattaaaaaa
     2281 ttaactgcaa ctgatgcttt tatttacgaa ctgatatcta aacgtatctt ctctactaag
     2341 gttgagtccg tcatttctag ctcaaccttc ctagaagcct atcaaggccg aatcggtgga
     2401 ttcacacttg gtcaatttga ccagggtggc ggtcgctgga tttcaggtgt caatcagttc
     2461 tctgttggta tggggaatgg tgccgggtat ggagtccgga cagccttctg ggcgaactgg
     2521 ggaaataatt ggaactatgc cggacctaaa gcatggaacg tcaatactga tgggaaaatg
     2581 tactgtagga atgaagtcgg tttttatgat caagtggatt tttcgaattc atcgagagca
     2641 aacttctatg ggaatactac tttttctcgt tctcctgtgt tttcaaatgg tatcgaactt
     2701 ggaagtaaag atgtgcttgg tgatggttgg aatcccaaag gcggaaggaa tgcggttgtt
     2761 tggtggaatc aggtcggtag cggtagcttg aagtattgga tggaacaaaa atcagataga
     2821 cgcttaaaag agaacatcac agatacagct gtgaaagcct tggataaaat caacagatta
     2881 agaatggttg catttgattt catcgaaaat aagaaacatg aggagattgg tctaatagct
     2941 caagaggctg aaaccatcgt tccaagaatt gtctcacgag atcctgagaa tccagatggc
     3001 tatctgcata ttgactatac cgctttagtt ccttacttaa tcaaggctat tcaagaatta
     3061 aatcaaaaaa tagaaaaaat ggagaaaaca atagcatgaa taacaacatg ttgaccaata
     3121 tcgcacttaa agcaattcag gagtttgctc ttgaaaatag aaaacgaaca cacagattgg
     3181 agaacttaga aaatgaacac agaacagctt aaccaagcct tacaaatgac aattcgtgaa
     3241 atgtcaacaa cttcaacaga ttcgatgatt gcaagtaatt tcttgagtat tcagttggat
     3301 gagcaaaggg aagagaatca aagacttcaa gcacgagtgg atgagctgga agctctgctt
     3361 gatgaacaaa ctaaaccagc agacaaagga gaatagacat ggcagaaaca attcaaaaca
     3421 cagataactt gctagacctt acaaaaatca cagaaccatt tgatcttgcg agcgctttgc
     3481 gctatatgaa agaaaatgga gagttcattc gttgcaagaa tgtaagcgat gacttctata
     3541 tgtatcgtga tgttcaaaaa cgtcctgtga tcgtaaatgg ccgtcgccaa ttcaaggatg
     3601 ttgaaaccgt ttgggcgttc aaccagtggg gtggtacaat cgcaacaatc aacgtagccg
     3661 ttctgttgaa tcatgaattc tatatcatga aatttgatgc agagggcaat cctgactgga
     3721 cggttccaac ggtagaacct aaagaatagg aggttgtatg ccaattgaag aagctgaaaa
     3781 aatcgctcaa agtcaggtag cttgggcgat tttgtttatc ttgctttttt ttattatcat
     3841 tcgatatctt atcaagactt cggacaagcg agagaagaag attatggatt tgcacgagca
     3901 atcaaaggcc gactctaata gacgagaaga gcgtttgatg actcacctag aaaagaccac
     3961 tacagaatta accacaatca ctcacacggt cggagacatt caaaaagaaa tggttcgcat
     4021 gaacgaccgc atggaagaaa tcgaaaaagg agaataacaa atgcaacaaa ttactgaaat
     4081 cattactaat ggagcaatca gcatcctagt cattttggca ggggtggtag ttagggtagt
     4141 caaggaatac ctcgtcaaaa aaggtggaga aaagactatc aagattgttg aaatcttggc
     4201 caagaacgca gttaatgccg tggagcaggt agctgctgaa actggctaca agggagatga
     4261 aaaactggca caggctcgcg ctaaagtccg tgctgagctt acaaaataca atattagcat
     4321 gactgacaaa gacttagaca ccttcgtaga gtcagcagtg aagcggatga atgacgcatg
     4381 gaaaggacga tagggaatgg atatcgatag aaacagacta cgtacaggct tgccacaggt
     4441 tggggtgcag ccttatcgac aagtacatgc tcactcaaca ggtaaccgca actcaaccgt
     4501 acagaatgaa gcggattatc actatagaaa ggaccctgaa ctagggttct tttcacatgt
     4561 tgtcggaaac ggccgtgtca tgcaagtagg acctgtgaac aacggaagtt gggatgttgg
     4621 gggcggttgg aatgctgaga gttacgcagc ggttgaactg attgaaagcc attcaactaa
     4681 ggaagagttt atggctgact atcgcctcta tatcgaattg ctacgcaatc tagcggacga
     4741 agcaggtttg ccgaaaacgc ttgatacagg gagtttagct ggaattaaaa cgcacgagta
     4801 ttgcacgaat aaccaaccaa acaaccactc agaccatgtg gatccatacc cttacttggc
     4861 aaaatggggc attagccgtg agcagtttaa gcatgatatt gagaacggct tgacgattga
     4921 aacaggctgg cagaagaatg acactggcta ctggtacgta cactcagacg gctcttatcc
     4981 aaaagataag tttgagaaaa tcaacggtac ctggtattat ttcgatggct caggctatat
     5041 gcttgcagac cgctggagga agcacacaga cggcaactgg tactggtttg acaactcagg
     5101 cgaaatggct acaggctgga agaaaattgc tgagaagtgg tactatttca acgaagaagg
     5161 tgccatgaag acaggctggg tcaagtacaa ggacacttgg tactacttag acgctaaaga
     5221 aggcgctatg gtatcaaacg ccttcgtcca gtccgcagac ggaacaggct ggtactacct
     5281 caaaccagac ggtagcatgg cagacaagcc agagttcaca gtagagccag atggcttgat
     5341 tacagttaaa taaatagaaa ggaaactttc taaattgttc tttcaccgca ggctcaggct
     5401 tgcggttttt ttgttttaaa aaaggggcaa aaaaggggca aaagtgtcgt aaatctctgt
     5461 aaaatgatgt aaaaacattt atttaaaagc tcaaaatata gctgttttaa aaggtattgt
     5521 aagatatagt aaaacgatgt aaaggtattt ttaaatgcgg atgaattata aagccacgaa
     5581 tcctatgtga ctagtggttc ttttttataa actggtagag tgttttggtt gtactttttg
     5641 ttcagggtca atgtagttga tagcgttgtt gacagcagtt ggagcttctc cgaggcctgt
     5701 cgcaatcaga tcaatttttc cgtcatagta gcagcagtca ccgatagcat agatacctgc
     5761 ttggctggat tcctgtttgc tgttgacgat aatcttgtga cggttgaggt tgagccccca
     5821 gtttttaagg ttaccgacag aagatttgaa accatagttg acaaagaggt ggtctaggtc
     5881 aatggtttca gtttcatcag atttgacttt tgtgatttca agtttatcaa gtgtttttcc
     5941 atttccaagg agttggctag gggcgaatgg tgtcttgatg gttacagatg attcttgcaa
     6001 ggcttgaaca ctgtgttcca aggcacggaa attatctctg cggtgaacaa gggtagttgg
     6061 tgcgattttt tcaaaagcca aagcccaatc cacagccgag tctcccccac caagaatcgt
     6121 cactttctta ccagcgtatt gctgaatgtt agaaacgtgg tagtggatat tttcatagcc
     6181 ctcaacacct tcaagttcca gcggacgtgg tttgaaggca ccgccaccca tagcgatgat
     6241 gactgtttta gtcaggtgac ttcctttaga agttgtgatg gcaaattctt cttcttgttt
     6301 gtcaatctca agaaccgttt cattgagatg aataggggta tcaaatccat ttagctgttc
     6361 aatcaagcgg ttagtcaact cttctccagt caggtttggg aagcctggta cgtctaggat
     6421 ttccttttca gggtagagaa tagcaggttg tccacctagc tggggaagag agtcgatgat
     6481 ttgaaccttg gcttggcgta ggtgggcata aaaggctgca aaaagcccga caggaccacc
     6541 acccacaatg gtaatatcat agagttgaga catagtttct cctttgtttt ttctagtcag
     6601 tttattttat catatttttg ttcaatgtaa aatggactag gatagggaaa aaaatgccag
     6661 aaaatggtat aataggacga aagtgttttg atagggagga tatcagggat gaattttcaa
     6721 caattatcca atctgcaata ttggaccagt ttgtttgcaa gtccatggac gatagctatc
     6781 aatctgattg atattttgat tgttgcttat attttatacc attttacaaa agctattgca
     6841 ggaaccaaga ttatgatttt ggtacgtgga gttttggtgt ttattttagc tcaaatcctt
     6901 gcaaatatga ttggtttgac tacgatttct tggttaatca atcaaattat tacttatggg
     6961 gttattgcgg cggttgttat cttctctcca gagattcgga ctggtttgga acgtttggga
     7021 agagcgacag atttcttttc caatgcccct attagtgctg aggaacagat gattcgtgcc
     7081 tttgttaagt ctgttgaata catgagtcct cgtaaaatcg gggccttggt tgctattcag
     7141 cgtgtacgta ccttgcagga gtatatttcg acaggaattc ccttggatgc taagatttct
     7201 gcagaacttc tcattaacat ttttattccc aacactcccc tacatgatgg tgcggtgatt
     7261 atcaaagaag aacgtatcgc tgtgacgtct gcctatctgc ccttgacaaa aaacacaggg
     7321 atttccaagg aatttgggac cagacaccgg gcggctatcg gtttatcaga agtctcagat
     7381 gccttgactt ttgtcgtatc agaggaaacg ggaggaattt cgataaccta taatggaagg
     7441 tttaagcaca acctaacact tgatgaattt gaaacagaat tacgtgaaat cttacttcca
     7501 aaagaggaag tgggtcttag ttttaaagaa cgattgctag gaggatggaa acatgaaaaa
     7561 aaatagttta tatatcatat cctcactctt ttttgcttgt gtcttatttg tctatgctac
     7621 ggcgacgaat tttcaaaaca gtaccagtgc taggcaggta aaaacggaaa cctatactaa
     7681 tacagtaaca aatgtcccta ttgacatacg ctataatagt gataagtatt ttattagcgg
     7741 ttttgcttca gaagtatcag tggtcttgac tggtgcaaat cgcctatcgc tagctagtga
     7801 aatgcaagaa agtacacgta aattcaaggt tactgctgac ctaacagatg ccggtgttgg
     7861 aacgattgaa gttcctttga gcattgaaga tttacccaat gggctgaccg ctgtggcgac
     7921 tccgcaaaaa attacagtca agattggtaa gaaggctcag aaggataagg taaagattgt
     7981 accagagatt gaccctagtc aaattgatag tcgggtacaa attgaaaatg tcatggtgtc
     8041 agataaagaa gtgtctatta cgagtgacca agagacattg gatagaattg ataagattat
     8101 cgctgtcttg ccaactagcg aacgcataac aggtaattac agtggttcag tacctttgca
     8161 ggcaatcgat cgcaatggtg ttgtcttacc ggcagttatc actccgtttg atacaataat
     8221 gaaggtgact acaaaaccag tagcaccaag ttcaagcaca tcaaattcaa gtacaagcag
     8281 ttcatcggag acatcttcgt caacgaaagc aactagttca aaaacgaatt aaaaatagaa
     8341 aaggatttta taacaatggg taaatatttt gggactgatg gagtccgtgg agaagctaac
     8401 ctagaactaa caccagaatt agcctttaaa ctaggacgtt ttggaggcta tgttcttagt
     8461 caacatgaaa cggaagcgcc gaaagtcttt gtaggacgtg acacacgtat ttcaggggaa
     8521 atgctggaat cggccttggt ggcaggtctc ctttcagtag ggattcacgt atacaaactt
     8581 ggtgtccttg caacaccagc agtagcttac ttggttgaaa ctgaaggagc aagtgccggt
     8641 gtcatgattt ctgctagcca caacccagcc cttgataacg gaatcaagtt ctttggcggt
     8701 gatggcttca aactagatga tgaaaaagaa gcagaaattg aagccttgct agatgctgag
     8761 gaagacactc ttcctcgtcc aagtgcagaa ggcttaggaa ttttggtaga ttatccagaa
     8821 ggcttgcgta agtatgaagg ataccttgtt tcaactggaa ctcctcttga tggaatgaag
     8881 gttgccttgg atacagctaa tggagcagct tctaccagtg cccgtcaaat ctttgcagac
     8941 cttggtgccc aattgacggt tatcggggaa acaccagacg gtcttaacat caaccttaat
     9001 gttggttcaa cacatccaga agcccttcaa gaagtggtca aagaaagtgg gtcagctatt
     9061 ggtttggcct ttgatggaga cagtgaccgc ttgattgctg ttgatgagaa tggtgacatc
     9121 gtcgatggtg acaagattat gtacatcatc ggaaaatacc tttctgaaaa aggacaattg
     9181 gctcaaaata caattgtgac aactgttatg tctaaccttg gtttccacaa ggccttgaat
     9241 cgcgaaggta ttaacaaggc agttactgca gttggtgacc gctacgttgt tgaagaaatg
     9301 agaaaatcag gctacaacct tggtggtgaa cagtctggtc acgttatctt gatggattac
     9361 aataccacag gtgatggtca attatcagca gttcaattga ctaaaattat gaaggaaact
     9421 ggtaagagct tatcagagtt ggcggcagaa gtaacgattt atccacaaaa attagttaat
     9481 atccgagtgg aaaacgtcat gaaggaaaag gccatggaag tgccagctat caaggccatc
     9541 atcgagaaga tggaagaaga aatggcgggg aacggccgta tccttgttcg tccaagtgga
     9601 acagagcccc tcttgcgtgt tatggcagaa gcgcctacaa cagaagaagt ggactactat
     9661 gttgatacca tcacagatgt agttcgtgct gaaattggga ttgactaaat cctagaaaac
     9721 tagatgaaaa taaaaaatta tggtacaata gcttataata ttgtagccga tggagaaaga
     9781 catgaatctt aaacgtgaac aagaatttgt tagtcagtat cattttgatg cacgtaattt
     9841 tgaatgggaa aatgaaaatg gagctcctga aaccaaggtg gatgtgaatt tccaattgct
     9901 ccaacatgac caagaaaatc aagtgacttc cttgattgtc atcttgagct ttatgattgt
     9961 atttgataag tttgtcatta gtggaacaat ctcacaagta aaccatatcg atggccgtat
    10021 tgtcaatgaa ccaagtgaat tgaaccaaga agaggtggaa accttggctc gtccatgttt
    10081 gaacatgctc aatcgtttga cttacgaagt gactgaaatt gcattagact taccaggaat
    10141 taatttggag ttctaatatg aaattagctg ttttcacaga ttcctctgct tatctcagtg
    10201 aagagacctt gcaaagagaa gacttgtttg tcttggatat tcctgtcaat attgatggtg
    10261 aggaatatgt cgaaggcatc aatctgtctg ctgaggaatt ttaccaaaaa atggctcagg
    10321 cttctgaatt gcctaagacc agtcaaccaa gtattgccaa gttagatgag atcttaactt
    10381 cgctcaaaga acaaggctat acacatgcct tggggctttt cctatcttct ggaatttcag
    10441 gtttttacca aaatatccag tatatggtcg atgactatga gggcttaacc attgctttcc
    10501 cagacacttt gattacaagt gctcccctag gcatcatggt tgaaagcgtc tttaattggc
    10561 gtgatcaggg cgatgatttt gccagcattc aggataagct agccattcaa atcagccgta
    10621 cgtcagcctt tatcatggta gatgacttgg atcatttggt aaaaggtgga cgcctttcaa
    10681 atggggctgc cattttgggc aatttgctta gcattaagcc aatcctttat tttaacgatc
    10741 aaggtgtgat tgaagtttac gaaaaagttc gtactgaaaa gaaggccacc aagcgcttaa
    10801 ttgaaattat caaggaaaca acggcttcag gtcaataccg ggtcattgtc attcatggga
    10861 atgctcctga aaaggctgaa gaattgcgtc agcacttgct tgattttggc ttgggttcgg
    10921 atgtttcact tgctacattt ggtagtgtca ttggaacgca cctaggagca ggaagtattg
    10981 ctctgggtta tattccagtg atttagttgg cgcttctagg ctagttaaga tgccttgtat
    11041 cttagaaatt gaacacggac tacctgcctc ttgaaaaaag atgcctgtct tgcctttcgt
    11101 ggaaagtcag tgccattccc tatttttcag aggcagctaa ggtcctttgt atcttgataa
    11161 ttgaacacgc ctggaaccct gtgtgaaaaa gatagttctt ccaagaagta gacacttctt
    11221 gattggaatt cctattttca ctttgtgttc ttacggactt tgtatcttag acaggagtag
    11281 agatgagtat tcgagtaatt attgccggtt ttaagggaaa gatgggccag gctgcttgtc
    11341 agatggtatt gactgatcca gacttggact tggtggcagt tttggatcct tttgagtctg
    11401 agtcagaatg gcagggtatt cctgttttca aggataaggc tgatttagct ggttttgaag
    11461 cggatgtctg ggtagatttt actactccag ctgttgccta cgaaaataca cgttttgctc
    11521 ttgaaaatgg ctttgctcca gtagttggaa cgactggttt cacgagtgaa gaaattgcag
    11581 agctaaaaga attttctcgt gcccaagact tgggtggcct gattgcccct aactttgcct
    11641 tgggtgctgt cttactcatg caatttgcga cgcaggctgc caaatatttc ccaaatgtgg
    11701 agattattga gctccatcat gacaagaaaa aggatgctcc gagtggaaca gccattaaaa
    11761 cagctgagtt gatggcagag gttcgagagt ccattcagca aggcgcagca gatgaggaag
    11821 agctgattgc tggtgctcgt ggtgctgact ttgatggtat gcgcatccac tcagttcgtt
    11881 tgccaggctt ggtagctcat caggaagtca tctttggcaa tcagggagaa gggctgaccc
    11941 tccgtcatga ctcctatgat cgcatctcct tcatgacagg agtcaatttg ggaattaaag
    12001 aagttgtcaa gcgtcatgag cttgtctatg gattagaaca cttattatga gattaacgca
    12061 aatgccttct gaatttcaga aggctttacc agtattagaa aaaattaaag aagcaggctt
    12121 tgaggcttat tttgttgggg gctctgttcg agatgccctt ctccatagcc ctatccacga
    12181 tgtggatatt gcgacgtctt cttatccaga agagaccaag cagatttttc cgcgaacagc
    12241 cgatatcgga atcgagcatg gaaccgtctt ggtcttagat ggggatgagg agtatgaggt
    12301 aacaaccttt cggacagagg atgtctatgt ggactatcgc agacccagtg cggtttcctt
    12361 tgtacgatcg ctagaagaag acctcaaacg ccgtgatttc acagtcaacg cctttgcctt
    12421 ggatgagaca ggagaaatcg ttgacttgtt ccatggttta gaagatttgg aaaagcaagt
    12481 cttgcgagca gttggagtgg ctagtgagcg tttcaacgaa gatgctttac ggattatgcg
    12541 tggtttccgt tttcaggcta gtcttggttt tgcacttgag ccagaaacat ttaaagctat
    12601 gaagaccttg acgccgcttt tggagaaaat ttctgtagag cgtaccttcg tcgagtttga
    12661 taaactcttg ctggctccat tttggagaag gggcttggct tccatgattg agagtcaagc
    12721 ttatgactat ctccctgata tggcatctag ccaggacaag ctcaacagac tgtttgattt
    12781 ggagactgat tttacttttg aatcctctga acaagcctgg gcggctttac tatgggcttt
    12841 ggagattgaa aatgcgcagt cacttttgaa atcttggaag acctcacgcc agtttgccaa
    12901 gcaagttcag gatttgctga ttattttggc tctgcgtgaa aatggagaat tgagcaagcg
    12961 agattgttat cgctttgaca tagatttgct tttacaggct gaaaatcttc gtcaagctca
    13021 aggaaaagaa gtcaacccac aagccatcac agaaaaatac caaagcttga ctatccatga
    13081 caagaaagag attcagatta atggcggtat tttgatcaag gaatatggct atcagccagg
    13141 cccagacttg ggagagattt taacagagat tgagtttgcc attgtcgatg gagaattgga
    13201 gaataatcgt gaagccatcc atgcttacct gagggagaaa aaatgagtga ttttatcgtt
    13261 gaaaaactaa gtaaatccgt tggtgacaag accgttttta gggatatttc ctttattatc
    13321 catgacttag acagaattgg tttaatcggt gtcaatggga ctggcaagac cacccttttg
    13381 gacgtccttt ctggtgtttc tggatttgat ggggatgtca gtcctttttc agctaaaaat
    13441 gattaccaga ttggttactt gactcaggat cctgattttg atgatagaaa gacagttttg
    13501 gatacggttc tatctagtga actcaaggaa atccagctca ttcgtgagta tgaattgatt
    13561 atgctcgact atagtgagga caagcaggcg cgtttggaac gtgtcatggc agagatggac
    13621 tctctccaag cttgggaaat cgaaagtcag gtcaagaccg ttcttagcaa attgggcatt
    13681 caagacttat ctactcctgt tggggaattg tcaggtggtc tgagaagacg ggtacagttg
    13741 gcacaagtct tacttggcaa ccacgacctc ttgcttttgg atgagccgac caaccatctg
    13801 gatattgcga ttattgagtg gctgaccctc tttttgaaaa attctaagaa gaccgtcctt
    13861 tttatcactc acgatcgtta tttcttagac gctttgtcaa cacggatttt cgagttggat
    13921 cgtgcaggct tgaccgagta ccagggaaat taccaggact atgttcgcct aaaggcggaa
    13981 caggatgagc gcgacgcggc tcttcttcac aaaaaagaac aactctacaa acaagaattg
    14041 gcctggatgc gcagacaacc gcaggcgcgt gcgaccaagc agcaggcccg tatcaatcga
    14101 ttccatgatc tgaaaaagga agtttcaggc agtagtgctg agacagactt gaccatgaac
    14161 tttgaaacca gtcggattgg gaagaaagtc atcgagtttc aggctgtttc ctttgcctat
    14221 gaaaacaagc ctattttgca agattttaat ctcttggtgc aggccaaaga ccgtatcgga
    14281 atcgttgggg acaatggtgt tggaaaatca accctgctca acttgattgc aggaagtctt
    14341 gagccaacta aaggacaagt tgtgattggg gaaactgttc gcatcgccta tttctctcag
    14401 caaattgagg gtttggatga aagcaagaga gttatcaatt acctgcagga agtggcagaa
    14461 gaggtcaaga ccagtggcgg ttctacgact tcgattgcag agttgctgga gcagttcctt
    14521 ttcccacgtt cgacgcatgg aaccttgatt gagaaattgt cagggggtga gaaaaaacgt
    14581 ctttatctcc tcaaactgct tttggaaaaa ccaaatgttc ttcttttaga cgagccaacc
    14641 aatgacctag atattgcaac tttgacagtc ttagagaatt tcttgcaagg ttttgcaggt
    14701 cccgttttaa cagtcagtca cgaccgctat ttcttggata aggtagcgac caagattctc
    14761 gcttttgagg atggcaagat tcgtcctttc tttggtcatt acaccgacta tcttgatgaa
    14821 aaagcttttg aaacagatat ggccaatcaa gtgcaaaagg tcgaaaagga aaaagtggtc
    14881 aaggttcgag aagacaagaa acgcatgacc taccaagaaa agcaggagtg ggcaagtatt
    14941 gaaggtgata ttgaaacctt ggaaaaacgt atcgctgcta ttgaagagga aatgcaggct
    15001 aacggctctg actttggtaa gctggctact ctccaaaaag aattggatga gaaaaatgaa
    15061 gcactccttg aaaaatacga acgctatgag tatctcagtg aatttgatag ttaagactat
    15121 tcgaaaatca aagtgcaaac tatgtcggct tcacattgcc gactcaagta cagcctgtga
    15181 ccggcttcct aatttgcact ttgattttta ttgagtataa aagaatagaa aaatcccgtc
    15241 tcatgaaagg atttctctat tcttttttgg tttcttgatg aaagatattt tgccaggttt
    15301 cgtggcgacg ccagatactg gtatggatga taccatctaa ctcatagcag atgagtttgg
    15361 ttttttgact gatggaagta atttgaatgt cctttatagc agaatttaac tcttttttgg
    15421 tcttataagc ctctttatcc atctgctctc catcttggcg aatatagaca aaatcatcag
    15481 tcaagagttc ttctagttgg tttccttggt caatcaattg ttcacgcata agcaattttt
    15541 tatagacatt gtctaaaatt tcatcctcag ggataggtgc tggttcgata tggacatcgg
    15601 tatcaaagac gccaaaacgc tcctccagca tagactcgac ctgatccgcg atttcatggc
    15661 tttcaaaaac agacaagtca ggattcatct ctagtgtaat atccaggtag atgttgctac
    15721 cgtaggtgcg acctctttgc gatttgacct tgctgatttt gggaatttcc atgatagcct
    15781 tttggtagtc ctcgagcagg cggtcgtcaa agccatctga aagactaaag gaagactcga
    15841 tgaagatatc ataggcagtc ttcaagataa agaaagtgat gatgatagca accagtttat
    15901 ccacaatcgg ataattgaaa ctactagcta ggatggcaat ggcggttcca agtgaggtaa
    15961 cagcgtcaga aagattgtcc ttagcagctg ccttcagcgc cttggagttg gatttcttac
    16021 tgaggcgagt attgtagaga tagaccacaa acataatcgc tgcagaaatg attcctagag
    16081 ttgcaccaag aggatcaatg accgtttctt cccgactgag aatcttttga atggtatctc
    16141 ttagaacatc gaaaccgaca tagaacatga tgatagaagt gatcaagctt gccaaatctt
    16201 caatcttcca atgaccaaaa cggtggtcac ggtctgcagg ctggcgcgcc atccgaatcc
    16261 cgattaagag ggccacattt ccaatgatgt ccgatacgtt attaaaacca tcggccacca
    16321 aactggatga atgaaggaga tgaccagctg ctaatttagc tgcagacaag atcaaatagg
    16381 tcgaaatact gataatggct ccacgctcag ctaacttgag atttgagata gattgcttca
    16441 ttcaacttcc tttctagtca catagcattc taccattata ctggttttca aggaaaaatt
    16501 caaataaggc agtttttact ctttgaaaac tccccaaaat ttggtataat ggtactactc
    16561 aagggagtag ctggcagaaa cctgtgatag tgtcgtcatt ccgaatttta tgctgaaaag
    16621 tatgctttcc ggccctatct taaacagcga gacttgttat gattaacagg tctctttttg
    16681 tttgtcgtga aaagatatga cagaaagaga gtctgttttg cacacaatgt caaggaggag
    16741 acacatgtca aaagaacaaa aacgccaagc gttttatact cagagccctg aagaggtctt
    16801 gcaggctgtg gatgcgaccg agcaaggttt gtcatcaagt gaggcggaaa agcgccttgc
    16861 cgaatttggg cacaatgaac tcgaagaagg cgagaaacga tcaatcttgg tcaaattcat
    16921 cgagcaattt aaggatttga tgattatcat cctagttgcg gcagcaatct tgtcagtcgt
    16981 gacttctggt ggggaagata tcgcagatgc cattatcatc ctagctgtgg ttatcatcaa
    17041 cgctgccttt ggtgtttacc aagaaggaaa agctgaagaa gctattgaag ccctcaaatc
    17101 catgtctagt ccagttgccc gcgttcttcg tgatggacac atggcagaga ttgactctaa
    17161 agaattggta ccaggcgata tcgttgccct tgaagcaggt gacgtggtac cagcggacct
    17221 acgtttgata gaagccaact ctcttaaaat tgaagaagca gccttgacag gtgaatctgt
    17281 accagtcgaa aaagacttgt cagtcgagct tgcgacagat gctggtattg gtgaccgtgt
    17341 caacatggcc ttccaaaact caaacgtaac ctatggtcgt gggatgggtg ttgttgtcaa
    17401 tacaggtatg tacactgaag ttggtcatat tgctggtatg cttcaagatg cggatgagac
    17461 tgatacacca ctcaaacaaa acttgaacaa cctttctaag gtcttgacct atgctatctt
    17521 ggtcattgcc cttgttactt ttgtagtggg tgtcttcatt caagggaaaa atccacttgg
    17581 tgagttgttg acttctgttg cccttgccgt tgcagccatt ccagaaggac ttcctgctat
    17641 cgttaccatc gttctttctc ttggtactca agttttggcc aaacgacatt ccatcgttcg
    17701 taagttgcca gcagttgaaa cacttggttc aactgaaatc atcgcttctg ataagactgg
    17761 tacgctgact atgaacaaga tgacagtcga aaaagtcttc tacgatgcgg ttctacatga
    17821 ctcagctgat gatattgaac taggtcttga aatgccacta cttcgttcag ttgtcttggc
    17881 caatgatacg aaaatcgatg tggaaggtaa cttgattggt gacccaaccg aaacagcctt
    17941 tatccaatat gccttggaca agggctatga tgtcaaaggt ttcttagaga aatatcctcg
    18001 tgtagctgaa ttgccatttg actctgaccg taagctcatg tcaacagttc acccattgcc
    18061 agatggtcgt ttccttgtag cagtcaaggg tgcgccagac caactcttaa aacgttgtct
    18121 tcttcgtgat aaggctgggg atattgctcc gattgatgag aaggttacaa atctcattca
    18181 tacaaacaat tctgaaatgg ctcatcaagc cttgcgtgtc cttgcaggtg cttataagat
    18241 tatcgatagt attccagaaa atctcacttc tgaagagctt gaaaatgatt taatttttac
    18301 tggtttgatt gggatgattg accctgaacg tcctgaagcc gctgaggctg ttcgtgtggc
    18361 taaggaagcg ggaatccgtc caattatgat tacaggtgac caccaagaca cagcggaagc
    18421 cattgccaaa cgtttgggaa tcattgacgc aaacgataca gaaggtcacg ttttaactgg
    18481 tgctgaactc aatgaactgt cagatgaaga atttgaaaaa gtcgttggtc aatactctgt
    18541 ttatgcccgt gtgtctccag aacacaaggt tcgtatcgtc aaggcttggc aaaaacaagg
    18601 taaagtcgtt gccatgacag gtgacggtgt caatgacgcg ccagctctga aaacagccga
    18661 tatcggtatc ggtatgggaa tcactggtac agaggtttct aagggggctt ctgatatgat
    18721 tcttgcagat gataactttg cgactattat cgtcgcagtg gaagaaggac gtaaggtctt
    18781 ctcaaacatt caaaagacta ttcagtacct actttctgct aatactgctg aagtattaac
    18841 catcttccta tcaaccttgt ttggttggga tgtcttacag ccggttcatc ttttgtggat
    18901 caacttggta acggatacct tcccagctat cgctcttggt gttgaacctg cagaacctgg
    18961 tgtcatgaat cataaaccac gtggacgcaa ggcaagcttc ttctcaggtg gtgttttgag
    19021 ttctatcatt tatcaaggtg tactccaagc agctcttgtt atgagtgttt atggccttgc
    19081 gattgcttac ccagttcatg tgggtgacaa tcatgctatt catgcagatg ccctaacgat
    19141 ggcctttgca acccttggtt tgattcagct cttccatgcc tacaatgtca agtctgtcta
    19201 ccaatccatc ttgacagttg gcccattcaa gtctaagacc tttaactggt ccatcttggt
    19261 atcctttatc cttctcatgg caacaatcgt tgtagaaccg cttgaaggaa tcttccacgt
    19321 aaccaaacta gacttgtcac aatggggaat tgttatggct ggaagcttct caatgattat
    19381 catcgtcgaa atcgttaagt ttattcaacg caaactcggt tttgacaaga atgcgattta
    19441 agggaggttt cggatgtcag catatcaatt accgaccgta tggcaggatg aagctagtaa
    19501 tcaaggagct tttacggggc taaacagacc aacagcaggt gcccgtttcg aacaaaactt
    19561 gccaaaagga gaacaagctt ttcagcttta ttcactggga acaccaaatg gtgtgaaggt
    19621 tactatctta ttggaagaat tactagaagc tggttttaag gaagcggctt acgacttgta
    19681 taagattgct atcatggatg gggatcaatt cggctcagac tttgtgaagc tcaatccaaa
    19741 ttccaagatt ccagccttat tggaccagtc aggtactgaa aacgtaagag tctttgagtc
    19801 tgctcatatt cttctttacc ttgctgagaa atttggagcc tttttaccaa gtaatcctgt
    19861 ggaaaaggta gaagttttga attggctatt ctggcaagca ggtgcagcac cttttctagg
    19921 tgggggattt ggacatttct tcaattatgc tcctgaaaaa ttggaatatc ctattaaccg
    19981 ttttacgatg gaagtgaaac gccagttgga tttattggat aaggaattgg ctcagaaacc
    20041 ttatattgca ggcaatgact atacgattgc agatattgct atctggtctt ggtatggaca
    20101 gttagttcaa ggaaatcttt accaaggttc tgcaaaattc ttggatgcct caagttatca
    20161 aaatctagta aaatgggcag aaaaaattgc caatcgtcca gctgttaagc gtggattgga
    20221 agtaacttat acagaaatta aatagcaaaa aagtcatctt cggatggctt tttgctacag
    20281 ttgagggaaa gggtttgcag ttatcggctt ttgtgctata attgctataa tatagtaaaa
    20341 agcaagagga gtgtgaggaa gtggaaaaga aaattgtgaa ggatatctta tttttatctc
    20401 aagtgtctca gccggcaagt caggaggacc tttatcttgc cagagatttg caggatacac
    20461 tcttagcaaa tcgtgatacc tgtgttggtc tagctgccaa tatgattggg gtgcagaagc
    20521 gcgtgattat ctttaatctt ggcttggttc ccgtggtcat gtttaaccca gtgcttctgt
    20581 cctttgaagg atcttatgag gcagaagaag gctgtttgtc cttggtaggt gtgagatcaa
    20641 ctaagcgtta tgaaaccata aggcttgcct atcgtgacag caagtggcag gaacagacca
    20701 ttaccttgac aggcttccca gctcagattt gccagcatga gctggatcac ttggaaggac
    20761 gaatcattta ggaggaaagc aaatgaaacg aatagtcttt gaacttattt ttatcgcaac
    20821 gacctggtat atctttttac cgccccttaa cctgaccagc tgggaatttc tcttcttcct
    20881 ctgtgggcat ttgttagttg tggcaatctt atttggcttt ggcaagggga taaaccttgt
    20941 caaaacggtt catgtgcgcc acggtaaggc ggaagctgcc ttaaatcttg agggtttcaa
    21001 aatcaatcgg ttagggaaaa ttctgttagc ttcgattgga ggaattcttc tcttggcagc
    21061 tttggtttcc ttggtaactt ccagcatgtt tcaggctaaa aattatgcca atgtagtcac
    21121 agttacggaa aaagacttta ctgaatttcc taagagtgac accagtaagg ttcctatcct
    21181 agatagaagt actgctgaaa aaattggaga ccgctacttg ggttccctaa ccgataaggt
    21241 gtcgcaatac gtagcggcag atacctatac ccaattgaca attgatggga aaccttatcg
    21301 ggtcacacca ctagaatatg cagaccctat caaatggttt aacaatcaag ccaagggaat
    21361 cggtgagtat attaaggtgg acatggtaac tggaaatgcg gatttggtgg acttgaagac
    21421 accaatcaag tattcagact cggagtattt taaccgtgat gtcaaacgtc acctgcgctt
    21481 gaagtacccg accaaaatct ttaaaactcc atcttttgag gtggacgatg agggcaatcc
    21541 tttctatgta gcaacggttt accaaaagca atttggactt gctgttcctc gtcctgcttc
    21601 agtcattatc ttggatgcta caaatggaga aaccaaggaa tacagcttat cagatgttcc
    21661 agaatgggtg gacaggatct atccagcaga ggaaaccatt gagcaaatca actacaacgg
    21721 caagtacaag gacggtttct tgaatgccat gatttccaag aaaaacgtga cccagactac
    21781 caatggctat aattacttgt ctatcggtaa tgacatctat ctctacacag gtgtgacgtc
    21841 ggctaatgcg gatgagagta atcttggttt catccttgaa aatatgcgaa caggagaaat
    21901 cactaagtat agcttggctt ctgcgacaga agaatcagcc cgtgaatcag cagaaggtgc
    21961 tgttcaggag aaatcctaca aagcaacctt cccaatcctc atcaacctca atgacaagcc
    22021 tctctacatc atgggcttga aggacaatgc tggcttggtc aaagagtacg ccctggtaga
    22081 cgcagtcgag taccaaaatg ttatcgttgc tactacagtg gaagagatgc tcagcaagta
    22141 tgccaataaa aacgaccttg aaattgacaa tgcaacgaca gaaagcatca agggagtagt
    22201 agcagacctc aaatcagctg ttatcaaggg agacactgtc tacttcttta aagttgatgg
    22261 caagatatac aaggtcaagg cttcagtatc cgatgacctt ccttaccttg aaaatggtaa
    22321 aaccttcgaa ggtcaagtag gaaaagacaa ttatctcaag acctttaagc tacggtaaaa
    22381 ataggttttt tcagaaagta tatgttataa tagagtaaat taagactaat ggaggacaaa
    22441 gaaatgggtt tttatttgat ggttgcttca atgctactcg gactgctcgc tttgaagata
    22501 ggtttttctc agttcaagga gaaaaaagat aaattcttat ctatcttaac aagtttagct
    22561 ggcacagccc ttgttctcgt ggctgtctgg ttaggatggc ccaaataaat agaaaaacct
    22621 cggtcaaacc gaggtttttc aaatgaattt cttggttgtg gctaaaaaat atgctacact
    22681 atcaatatga aaattttaat cccaacagca aaagaaatga acacagactt gccaagtatc
    22741 gaggcaattc ctttaaaacc agaaagtcag accgtgcttg atgccttggc tctctattct
    22801 gccagtcaat tggagagttt ctacaaggta tcagctgaga aagcggcgga agaatttcaa
    22861 aatatccaag ctttgaaaag gcaaactgct caacactatc cagccttgaa actttttgat
    22921 gggcttatgt accgcaacat taagagagat aagctgaccg aggcggaaca agattatctt
    22981 gaaaatcatg tcttcattac ctcggctttg tacggtgttg ttccagcctt gtcacccatg
    23041 gctcctcacc gtttggattt tttgatgaaa ttaaaagtcg ctggtaagac tttgaagagc
    23101 cattggaagg cagtctatga tgaagctctg aagaaggaag aagtgatttt ctctctcttg
    23161 tcatcagagt ttgagactgt attttctaag gaaatcagag caaagatggt gaccttcaaa
    23221 ttcatggagg atagaggcgg tcagctgaag attcactcaa ctatctccaa gaaagcgcgc
    23281 ggggcctttc taacagcttt aatagaaaat caagtacaaa ctgtggggga agcacgtcgc
    23341 ttgaactttg ctggatttgt ttaccgagaa gatttgtcac aaccacaggg attggttttt
    23401 gtaaaggaag tatagaaatc agataagaga aaagtcagca cgattatgct gacttttttg
    23461 ataacttata aagtattgga caaatgaatc tcatttgata aggtgttaag atgattaacc
    23521 aaatttatga ttgacagatg atttcggcaa ataaacttct tggtaatccc tggaatatag
    23581 aaattagaca cgatgatatc gtaatctgtc tgatttaaaa tctcaggact tgtttttaat
    23641 tcatcccaaa tatcgaaggt aaagcggtta ttacagtaat aggaaagcat atcaacaaag
    23701 gtaagggata tagcatggtc aaagttactg ataatcaaaa ccttgatagg tggtcgattt
    23761 tctaacaact gagtagagag gttttcagca tgggttaaga tggtgtacat caagtgttcc
    23821 aactgttcag gatggtcatg ctgtccaatt gcctgacggt attgagccaa ttcttgacgc
    23881 gcacttccca taaagtctgg gaagtaaact tcaaattttt taatcgtcaa tgctttttgt
    23941 tcaaataaga ttggggtaga aaagatttcc tgtctttcaa agaaagcagt gttgtgtaaa
    24001 tgccaaatca gctcatcgtg gttagtaaaa gtaatcttat attgttttga taggcgttct
    24061 aatatttgac taagtaattg gtaagagtat cttgcttgac tgttatcttc taaagagttg
    24121 aagaattctt gaggatctag gaaaatatca ttctgaagaa aagaaataaa aatttgagcg
    24181 attgtatctg gagtcatttc aagcccaaaa tgtttagaga aataagcaag tgtctcctgg
    24241 aaatttggaa tgctctttaa aagcggataa tattctttgt aaaagtggtt ggggaggtca
    24301 atgaagtgac catttttaac cctgtggata ctgatagcta tcatcagttt gtacattcta
    24361 taaattgaaa atcgcattgg ataattcgtg attttataga agaagtcagc aaactctgtt
    24421 aaatcttcct caggaaggtc aggaaaaggc caatctagga aataatagcg ctcagaaaag
    24481 tattgggcaa aaaagtaacg aatatcgatc tcatttccac gtatctcgga tggagtaaaa
    24541 gataattcaa tttgaaactg agatgaaaga acctttgtaa tatttctgcc cagtcgatag
    24601 aggttggcac tactaaagta aagattttct attgttcgat aaataggtag cccctcatt
//
LOCUS       DAVHIU010000020        22410 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVHIU010000020 DAVHIU010000000
VERSION     DAVHIU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.021"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..5618
                     /locus_tag="VVN06_000666"
     CDS             <1..5618
                     /locus_tag="VVN06_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5562262.1"
                     /translation="HSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASVQT
                     VQAEENKQETTNSISVGRGEAATKLAEVSASNKEKTYAAPTVANPVETTPVKTGEVTK
                     PAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAGQS
                     LLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETNPA
                     ATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDLDKGY
                     SGFRAAPAPTNKLVTNLGNNTVFTDISRGSHTFRGNGNSRGGNPIHFDVTTTRVGNRV
                     EFSIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSSYS
                     LTSAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNYYF
                     REKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIYKV
                     HQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFYLD
                     NDGNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKDNK
                     NDGTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNELD
                     ASDKKLKEVIIADGAKGEQGLQGRDGAQGPKGEQGNPGTPGKDGKSLIAVKNGTETKV
                     YVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGR
                     PQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDG
                     KDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGE
                     KGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDV
                     FVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTV
                     NVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMI
                     KDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTV
                     TAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAIGQDGQTPTITTQRGQDG
                     QSTVVTITTPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRATDAAATPTRNG
                     IRVTVYDDVNDNGVYDGGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQN
                     PDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKV
                     EVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTK
                     NEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDE
                     QNGTHKISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVENGNGTVSETTVRDG
                     KSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRYGREPKLEVIDNNDGSHTIKVTGADG
                     KETTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDGVSPTVTVE
                     NNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTKT
                     VIKDGKDGRDGRDGRDGKDGKCGCQDKPVTPSNDKPVPPAPNVPTPEVLVKPVPAQPT
                     PNVPTPEVPVQPTPAVPTPEVPVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLP
                     ETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            complement(5855..6403)
                     /locus_tag="VVN06_000667"
     CDS             complement(5855..6403)
                     /locus_tag="VVN06_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5562263.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            6724..7212
                     /locus_tag="VVN06_000668"
     CDS             6724..7212
                     /locus_tag="VVN06_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU5562264.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            7209..8396
                     /locus_tag="VVN06_000669"
     CDS             7209..8396
                     /locus_tag="VVN06_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5562265.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEVA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEESEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            8389..8613
                     /locus_tag="VVN06_000670"
     CDS             8389..8613
                     /locus_tag="VVN06_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU5562266.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            8770..10116
                     /gene="asnS"
                     /locus_tag="VVN06_000671"
     CDS             8770..10116
                     /gene="asnS"
                     /locus_tag="VVN06_000671"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU5562267.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            10269..10559
                     /locus_tag="VVN06_000672"
     CDS             10269..10559
                     /locus_tag="VVN06_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5562268.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            10571..11041
                     /gene="ssbA"
                     /locus_tag="VVN06_000673"
     CDS             10571..11041
                     /gene="ssbA"
                     /locus_tag="VVN06_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU5562269.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            11073..11312
                     /locus_tag="VVN06_000674"
     CDS             11073..11312
                     /locus_tag="VVN06_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5562270.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            11607..13007
                     /locus_tag="VVN06_000675"
     CDS             11607..13007
                     /locus_tag="VVN06_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU5562271.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIGKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            13009..13368
                     /locus_tag="VVN06_000676"
     CDS             13009..13368
                     /locus_tag="VVN06_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5562272.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            13422..14171
                     /locus_tag="VVN06_000677"
     CDS             13422..14171
                     /locus_tag="VVN06_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU5562273.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            14161..14460
                     /locus_tag="VVN06_000678"
     CDS             14161..14460
                     /locus_tag="VVN06_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5562274.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            14531..15466
                     /locus_tag="VVN06_000679"
     CDS             14531..15466
                     /locus_tag="VVN06_000679"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU5562275.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            15553..16065
                     /locus_tag="VVN06_000680"
     CDS             15553..16065
                     /locus_tag="VVN06_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5562276.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            16090..16698
                     /locus_tag="VVN06_000681"
     CDS             16090..16698
                     /locus_tag="VVN06_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU5562277.1"
                     /translation="MFGELIRYLDQYEDVILREIKSQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            16799..16993
                     /locus_tag="VVN06_000682"
     CDS             16799..16993
                     /locus_tag="VVN06_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5562278.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(17039..18484)
                     /locus_tag="VVN06_000683"
     CDS             complement(17039..18484)
                     /locus_tag="VVN06_000683"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU5562279.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKILAFTGTKGKTTAAYFAYNILSQRYPTALLSTMNTTLDGTTFFKSSFS
                     TPENIDLFDMMAQAVKNGRSHLVMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            18590..20212
                     /locus_tag="VVN06_000684"
     CDS             18590..20212
                     /locus_tag="VVN06_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5562280.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            20413..22374
                     /locus_tag="VVN06_000685"
     CDS             20413..22374
                     /locus_tag="VVN06_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562281.1"
                     /translation="MKKSKSKYLTLAGFVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQDALVRNFTDGAYSYARLYP
                     NSSSFEGIKEKYKDDIIYSMQDATSYYWNFNLDRQAYKFTSKTTDIEKKSTQEAVLNK
                     NFRQAINFAYDRTSYGAQTQGEDGATKILRNLVVPPTFVSIKGKDFGEVVASKMVNNY
                     GKEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDATVDQASKKGIQE
                     VSSMKQSIEAALGTENVVIDIQQLSTEDYDNSSYLAQTAIQKDYDLYNGGWSPDYLDP
                     STYLDIFSVKNGGVLQNLGLDPGEANDKAKAVGLDTYTQMLEEANKEQDPAKRYEKYA
                     DIQAWLIDSSLAIPNVSLGGTPSLRKTVPFSAPYSLAGNKGVESYKYLKVQDKIVTTD
                     EYAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT         7053 a         4177 c         4990 g         6190 t
ORIGIN      
        1 aacattcaca taaaaaatca tttgactggt atagcatgca acaacgttat tctattcgta
       61 agtattactt tggtgcagtt agtgtcttgc tcggtaccgc tttggtatta ggtgcagcag
      121 ctagtgtcca aacagtacaa gcggaagaaa acaaacaaga aactaccaat agtatttctg
      181 ttggtagggg agaagcagct actaaactag cagaggtttc tgcgtctaat aaagagaaaa
      241 cctatgcagc tccaactgta gctaatccag tagaaacgac tccagttaaa actggagaag
      301 ttactaaacc agcagaaaaa gttgaagaag caaaagacaa aaaagaggaa gtaacgcatc
      361 aagatgctat tgacaagtca aaattgttaa cggctctttc gcgtgctaaa aagttagaaa
      421 gcaagttata tacagaagca agtgctgcta acttgcaaac aagtatccaa gctggtcaaa
      481 gcttgcttgg gaaagcagat gcatctgaag ctgaattatc agcagcagag tcatctattc
      541 aatcatctat tattggtcta gaacttcgtt ctaactctaa caaaggaact gtatcagaaa
      601 ctcctgtagc gaagaaagct aatatagttg aagcgaagga agagactaat ccagctgcaa
      661 caactgatcg ttcagctgtt gatagtgctg ttttgccaac tagcacagct gccaaagtag
      721 aaacaacttc agctccagca tctattaatg aaatcttgaa accaggtttg agcctttctg
      781 atgctcgcca aaatccagct atccgtaaag aagacttgga taaggggtat agtggtttta
      841 gagcagcacc tgctccaacg aacaaactag taactaatct aggaaataat actgttttca
      901 ctgatattag cagagggtct catactttcc gtggtaatgg gaattcacgt ggtggaaacc
      961 cgattcattt tgatgtaaca acaactagag ttggaaatag agtagaattc tcaattgcat
     1021 attctggtcc aggtgagttc gttaataata actttctctt agacaaaggg aatggatttg
     1081 gagaaccatc tcgtgcaact atttcaacaa acagattaag agatcaaact aaagatataa
     1141 gaaagggtgc aaattttgta tctcattcaa gctacagttt aacatctgct gttgaaacaa
     1201 acagtaatca aactattaga tttagtttac ctatagccaa ccctaatggt gatctgtctg
     1261 ttcgattaag acctgttact tttaatgtag gtcaagggga atcaggatct gcaactagta
     1321 atgatccata tagtaactct aactattact tcagagaaaa accactcctc ttggatgcaa
     1381 atccaaatgg tggtactaat aataagactg tttcagaaga cattgacttc caaactgtct
     1441 atcttccaac tagtaagtta ccagaaggtc aaactagatt agttcgagaa ggtgaaaaag
     1501 gacaacgtca aatcatctat aaagttcatc aattgggtaa cgaaacaatc ctaggattgc
     1561 caattagtaa ttcagttatt aaagaagtta agccacgtat tatgcaaatt ggtgtggcta
     1621 aagatctaat cgatacagta aaaccacgtg ttgatcaaaa taaagtcggt gatacaaata
     1681 acctcacttt ctatcttgat aacgatggaa acggtgttta tactgagggc gtagacgaac
     1741 ttgttcaaaa aattgctatt aaagatggtg ctaaaggtga aaaaggagtc caaggcgaac
     1801 gcggcctgac tggagcgcaa ggtcaggcag gtcgtgacgg tgtaactcca accgtaaccg
     1861 ttaaagataa taaaaatgac ggcactcata ctatcactat taacgacggt agaggtaatg
     1921 ttgcaagtac tgttgtaaga gatggtttcg atggcgcaag tccattagtt gcgactcaac
     1981 gaaatgaagc agataagaca acaactgtta tcttctatta tgatcaaaat ggaaataatg
     2041 aattagatgc ttctgataag aaattaaaag aagttattat tgcagatggt gccaaaggtg
     2101 aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa aggagaacaa ggtaatccag
     2161 gaactccagg taaagatgga aaatctctaa ttgctgttaa aaatggaact gaaacaaaag
     2221 tatatgtaga ggatccggct agaccaggac aaccacttaa tccaaaccaa ccattagcaa
     2281 ctatcactga tggaagaaat ggtactaatg gacaatcacc aacaattaca gcgacacgaa
     2341 gtgttcaaaa tggtaaaaat ggtgtattag taacaattac accagtagga ggtcgtccac
     2401 aaacaacatt tgtagaggat ggacaaaaag gtgctgatgg gaaaacccca acagtaacaa
     2461 taactgaggg gcaaaacggc acacatacat taacggttca taatccagga agtccagatg
     2521 tgacaactac gatccgtgat ggagctacag gacaagcagg tcgtgatggt aaagatgtat
     2581 taaacggaaa agtaaatcca caaccaaacc aaggtaaaaa tggagataaa tatattaata
     2641 ccgaaaccgg tgatgtctat gttaaaaaca atggaaactg ggataaagaa ggcaacatca
     2701 aaggccctaa aggggacaaa ggtgcagatg gtgctaaagg cgaaaaagga gaccaaggcg
     2761 aacgcggcct aactggagcg caaggagcta aaggtgcgga tggcgcagta ggtcgtgatg
     2821 gacgtgacgg taaagacgtg ttgaacggca aagctaaccc agaagcacat caaggtaaag
     2881 acggcgataa atacgttaat acagaaacag gcgacgtctt cgttaagaat aacggcaact
     2941 gggataaaga gggcaacatc aaaggcccta agggtgacaa aggtgaacgc ggagaagatg
     3001 gtaagactcc agaagtaact gtaactccag gtaaagatgg ccatagtact gacattacat
     3061 tcactgttcc aggtaaagat ccagttacag taaatgttaa ggacggagaa aatggtctga
     3121 acggtaaaac tccaaaagtt gatttacttc gtgtccaagg aaaaaacgga aatccatctc
     3181 atacaattgt gacattctat acagatgaaa acaatgacgg caaatataca ccaggaactg
     3241 atgaacttct aggttcagaa atgattaaag atggtgctaa aggcgcggac ggacgagatg
     3301 gtaaatcatt gcttactgtc aaggatggta aagaaactaa agtttaccaa gaagatccag
     3361 ctaacccagg acaaccatta aatccagaaa aaccacttgc ggtaattaga gatggagtag
     3421 atggaaaatc acctacagtt acagctgttc gtaaagatga agcagggcat aaaggtgtag
     3481 aaatcactgt tgataaccat gatggttcac aaccaactac agtctttgtt caagatggtg
     3541 ctaagggaga aactggtgca atcggtcagg atggacaaac tcctacaatc actactcaac
     3601 gtggacaaga tggccaaagc actgttgtaa ctatcacaac accaggtaaa gatccagtaa
     3661 ccttcactgt aaaagatggt aagaatggta aagatggccg tgcaccgaaa atcaaagtag
     3721 aagatattac ttcaccttca agaattagac gcgctacaga tgctgctgca actccaacgc
     3781 gtaacggtat ccgtgttaca gtttatgatg atgttaatga caatggggta tacgacggag
     3841 gtgtcgataa agtattaaat agtaaagata tttataacgg tatagatgga cgtgatggtt
     3901 cagctccaac tattactaca aaagataatg gagatggaac acacactatc acagttcaaa
     3961 atcctgatgg ttctgaatca acaactgttg ttaaagatgg taaagacggt aaaactgcga
     4021 atatcactac aacagaaaac ccagatggaa gccacacaat tacggtgaca aatccagatg
     4081 gttcaactaa agaaactgtt gttaaaaacg gtaaagacgg taagactcct aaagttgaag
     4141 taacggataa caacgatgga actcacacag ttaaagtgac ggatggagac ggtaatgtaa
     4201 cgaacgctat catcaaagat ggtaaagatg gtaaagctgc aacagcaaca actactgaaa
     4261 atccagatgg aagccacaca gtaacaatca ctaacccaga cggaactaag aatgagtttg
     4321 ttgttaagaa tggacgtgac ggtgttgacg gacgtactcc aaccgcatct gttcgtgata
     4381 atggagacgg aagtcataca atcgttatta caaatccaga aggtgtgaca actgaaacca
     4441 cagttcgtga tggtaaatca ccaaaagtga ctataactga tgaacaaaat ggaactcata
     4501 agatctctgt tctaaatggt gacggaacaa ctactgaaac aatcattaaa gatggtaaat
     4561 caccagtagc aacagttaga gataaccaag atggtactta cactattcgt gtggaaaatg
     4621 gtaatggtac tgtttctgaa accacagttc gtgacggtaa atcaccaact gctaaggttg
     4681 tggataacgg agatggaaca cacactatca cagttgtgaa ctcagacgga acaactacaa
     4741 caactacagt tcgttatggt agagaaccaa aacttgaagt tattgataac aacgatggtt
     4801 cacacactat taaagtgaca ggtgctgatg gtaaagaaac gacaactaca atctttgatg
     4861 gtaaatcacc aaaagcgaac atcgttgata acggagatgg aactcataca ttaacaatcg
     4921 tagattctga tggtcgtgaa tacaaatcta ttattaaaga tggtaaagac ggcaaagatg
     4981 gcgtttcacc aactgtaact gttgaaaata ataacgatgg aactcacgtt gttacaatca
     5041 ctaatccaga tggaagtaag acagaaatgg tgattaaaga cggtaaagat ggtaaatcac
     5101 caaaagtttc tgttgaagat aatggtgatg gtagtcatac aatcacaatc atcaattctg
     5161 atggaactgt gacaaaaaca gttattaaag atggcaaaga tggtagagat ggacgtgatg
     5221 gtagagacgg caaagatgga aaatgtggat gccaagacaa accagtaaca ccatcaaatg
     5281 acaaaccagt acctccagca ccaaatgtgc cgacaccaga agtactggtt aaacctgtgc
     5341 cagcgcaacc aacaccaaat gtaccgacac cagaagtgcc agtacaacca actccagctg
     5401 ttccaacacc agaagtaccg gttccagaac aaccagtagt accaacaccg gctcaaccag
     5461 caactccagt aaatgctaat ccagtagcac caactacagg taaagaaaac cgtggggata
     5521 aattacctga aactggaagc caatctgatt atatctctgt tcttttaggt agcggtattc
     5581 tattgagcct atatgtagga cgaagaaaag aagattaatc agccaaaagc agtcactaat
     5641 ttctcgaaag aagatgataa gtgatagatc tcgaatgaaa gaatattctt agaatgattt
     5701 tcctatgatg gaaaagactg ctaaaaacct tgagtggtaa cgctcaaggt ttttttggat
     5761 ggctgatgtt tgtctgaaaa tcttgattga gaaataaaaa gccaccctat taggtggcta
     5821 taattgattt agactttcta aaattttcta ttttttactg aatcgcttcg accaagtctt
     5881 gagcttgttt ttcaagtgag tttaggactg tttcttcaag aaccaatttt ccgtctgccc
     5941 aggcagagtc attaacacgt gcagcagtga aatcaccaac gccttgtgta cggataaatg
     6001 gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc tgcattggct acagatgata
     6061 ctgtgacaaa cttgtcttga agggcagaag cgccacgtgt atcagacaag tcaagggcac
     6121 gagatagcca gtcaagcaag ttttttactg taccagggat agagaagttg tagactggag
     6181 agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg agcagcagct acagctggat
     6241 gtgttggaac ttccaaatct tggctgaaga gaggaagggc tgaataatca aggtagctaa
     6301 cttccgcttt accagcaagt gctttctcag cttcgagcgc catttggtgg ttgaaagaac
     6361 cttgacgtag tgatccgacg ataaataata cttttttaga catgatgtgt ctcctttttc
     6421 ttcaaatttc gagaactctc tcgttgttat aaaatatatt acaacaaaac aaaagaactt
     6481 gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat ttcggaaact ctagtcattt
     6541 gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg ttttgtttga agggagaaat
     6601 agaattggtt gctcaaagca cagctttgag gttgcagata aagctgacgt ggtttgaaga
     6661 gattttcgaa gagtattctt atattcactt gtaataaagc ttaatagatg gtaaaataga
     6721 cgagtgaaaa aaagacaaaa caaagcaaaa aataatctac tgtggcagta tggtctaggg
     6781 atgacgattt tgtttgtggt tatcagtgct tcctttctgt atatggtttc tcttagcatg
     6841 aaaccctatc aaacagctaa aagtgaagga gaaaaattag ctcagcagta tgcaggatta
     6901 gagcaggccg atcaggttga tttatacaat ggcttggaat cttattacag cgttcttggt
     6961 cgtaataaac agcaagaagc acttgctgtt ctgattggaa aagatgatca taagatttac
     7021 gtttatcagc taaatcaggg tgtttcacaa gaaaaagcag aaacggtttc taaggaaaag
     7081 ggagctggcg aaattgacaa gattatcttt ggtcgttatc aagataagcc aatctgggaa
     7141 gtcaagtcag gatctgattt ttatctagta gattttgaaa caggagcatt ggtcaacaag
     7201 gagggcctat gaaattatcc aaccgtgttt tagaaatgga agaaagtgtg actttggctg
     7261 ctggagcccg tgccaaagca ctgaaggctg agggcagaga tattttgtct ctaaccttgg
     7321 gtgagccaga tttcactact cccaaaaata tccaagatgc cgccattgca tcgattcgag
     7381 atgggcgtgc ttctttttat acagtaacct caggtctgcc agagcttaag gcggtggtca
     7441 atagctactt tgagcgcttt tacggctatt ctgtagcgtc aaatcaagtg acagtcgctg
     7501 cgggagccaa atattctctc tataccttct ttatggctgt ggtcaatcca ggtgatgaag
     7561 tcatcatccc aaccccatac tgggttagct atggagatca ggtcaagatg gcagaaggcg
     7621 ttcccgtctt tgtttctgct aaggaagaca atcactttaa ggtgaccgta gagcagttag
     7681 aagtagctcg cactgacaag accaaggttt tggtgctgaa ttcgccatct aatcccacag
     7741 gtatgattta cacccgtgag gaactcttgg caattggaaa ctgggctgta gaaaatgata
     7801 ttctcatcct agcagacgat atctatggcc gcttggttta taatggtcat gagttcacac
     7861 cgatttctag cctatcggaa gcgattcgca agcaaacagt ggtcatcaat ggtgtgtcta
     7921 aaacctatgc catgactggt tggcggattg gttatgccgt tggagaagca gacattatcg
     7981 ctgccatgtc caagattgca ggtcaaacaa cttcgaatcc gtcagcagta gcccaatatg
     8041 cagcagttga ggctctatca ggtgagcaag atactgtaga aagcatgcgt caggcctttg
     8101 aggaacgtct taataccatc tatcccctcc ttgcagaggt gccaggattt gaagtggtca
     8161 aaccgcaagg ggccttctat ctcttcccaa atgtcaaaaa ggccatggag atgaaaggct
     8221 acacggatgt gacagacttt acaactgcta tcttagaaga atccgaagtg gccttggtaa
     8281 caggagctgg atttggagca ccagaaaatg tccgcctcag ctatgcgaca gacctagaca
     8341 cgcttaaaga agcagtcgaa cgcttgaaag catttatggg tagtgagaat gattgatcat
     8401 tttgagatta aggtaaagga tttacaaatt tcagaaggat tttataggag ttttctcgct
     8461 cctttggact ataaattggc ttttaagact agttctctaa ttagttttct atccccgaac
     8521 agccctcatc ctggtggtga tttttggctg actcagggaa cacaagatcc tgttcatttt
     8581 gcttttttag cagaaaataa ggaagaagtt taggcttgtt atgaggctgg cttagaggca
     8641 ggtgggcgag acaatggggc tcctggttat cgaagtgagc gtccgattta ctatgctgct
     8701 tttatgattg acctggatgg gaacaatata gaggtggttt gccataaaga ataaaaaaga
     8761 aaaggaaaaa taatgacaaa acgtgtaacg attattgacg taaaagacta tgttggtcag
     8821 gaagtgacga ttggcgcttg ggttgccaat aaatcaggaa aagggaaaat cgctttctta
     8881 caattgcgtg atggaacagc cttctttcaa ggtgtggctt ttaaaccaaa ctttgtcgaa
     8941 aaatttggtg aagaagtggg acttgagaag tttgatgtta tcaaacgctt gagccaagaa
     9001 acgtctgttt atgtgacagg tattgtcaaa gaggacgaac gttctaaatt tggctatgag
     9061 ttggacatca cagacatcga agtgatcggt gaatctcaag actacccaat cacaccaaaa
     9121 gaacacggaa cagacttttt gatggataac cgtcacttgt ggctacgctc tcgtaagcaa
     9181 gtagctgtgt tgcaaatccg taacgctatt atctatgcaa cttatgagtt ctttgacaag
     9241 aacggtttta tgaagtttga cagcccaatt ctttcaggaa atgcggcaga agattctaca
     9301 gaactctttg aaactgacta cttcggaacg ccagcctact tgagccaatc aggtcaactt
     9361 tacctagaag caggggctat ggctcttggt cgtgtctttg actttggtcc agttttccgt
     9421 gctgaaaaat caaaaacacg ccgtcacttg actgagttct ggatgatgga tgctgagtac
     9481 tcatacttga cacatgatga gtcgcttgac ttgcaagaag cttatgtgaa agctcttcta
     9541 caaggtgttc ttgaccgcgc gcctcaagcc ttggaaacct tggaacgtga tacagaactc
     9601 ttgaaacgct acattgcaga gccattcaaa cgtatcactt acgatcaagc cattgacctc
     9661 ttgcaagagc atgaaaatga tgaagatgct gactacgagc atcttgagca tggtgatgac
     9721 tttgggtcac cacacgaaac ttggatttca aaccactttg gtgtgccaac atttgtcatg
     9781 aactatccag cagccatcaa ggccttctac atgaaaccag ttcctggaaa tccagagcgc
     9841 gtgctttgtg cagacttgct tgctccagaa ggctatggag aaattatcgg tgggtctatg
     9901 cgtgaggaag attacgatgc ccttgtcgct aagatggatg aacttggcat ggatcgtaca
     9961 gaatatgaat tctaccttga ccttcgtaaa tacggtacag ttccacacgg aggatttggt
    10021 atcggtatcg aacgtatggt aaccttcgca gcaggaacaa aacatatccg tgaagctatt
    10081 ccattcccac gtatgttgca ccgtatcaaa ccataagaac aggaaaacgc ccatgtggcg
    10141 ttttttcgaa ttctagtgtt tttacttgcg taaaaaaatt ttttctagta taatagttta
    10201 ttgtgagcga acctcactta ccccttgcaa agtcttgggg tcattagacc aaaaggagga
    10261 acatatcaat ggctaaatac gaaattcttt atatcattcg tccaaacatt gaagaagaag
    10321 ctaaaaacgc tttggtagca cgttttgact ctattttgac tgacaacggt gcaactgttg
    10381 ttgaatcaaa aacttgggaa aaacgtcgtc ttgcatacga aatccaagat ttccgtgaag
    10441 gactttacca catcgttaac gttgaagcaa atgacgatgc agctcttaaa gagtttgacc
    10501 gtctttcaaa aatcaacgct gacattcttc gtcacatgat cgtcaaaatt gacgcgtaag
    10561 aaggtaaact atgattaaca atgttgtact tgtagggcgt atgacacgtg acgctgagtt
    10621 gcgttatacc ccatcaaatg tagcagttgc gacttttact cttgcagtaa accgtacatt
    10681 taagagtcaa aatggtgaac gtgaggctga ttttatcaat gtcgttatgt ggcgccaaca
    10741 ggctgaaaat cttgctaact gggctaaaaa aggctcactt atcggggtga caggtcgtat
    10801 ccagactcgt agttacgata accagcaagg acaacgtgtc tacgtgacag aggtcgtggc
    10861 tgagaatttc caaatgttgg aaagccgtag tgtgcgtgag ggtcacacag gtggagctta
    10921 ctctgcacca actgcaaact attcagcacc tacaaattca gtaccagact tttcacgtaa
    10981 tgaaaatcca tttggagcaa caaacccatt ggatatttca gatgatgatt taccattcta
    11041 atggacaact aaacttataa aggagaaaaa acatggctca acaacgtcgt ggcggattca
    11101 aacgccgtaa aaaagttgat tacatcgcag caaataaaat tgaatatgtt gattacaaag
    11161 atactgagct tcttagccgt ttcgtttcag aacgtgggaa aatccttcct cgtcgtgtaa
    11221 caggaacttc agctaaaaac caacgtaaag taacaacagc tatcaaacgc gctcgcgtaa
    11281 tggctttgat gcctttcgta aacgaagatt aaagaaaaga cccggttggg tctctttttc
    11341 atgagcttga aaacaagaaa gcgaattaag accccaacgg gtcttttttc acaagctttg
    11401 aaatgagaga gcgagttagg ctcagtggct ccctttttac gagtttgaaa acaagagaac
    11461 acattacaag cacgatggag ctttttcata aatatgtctc ttttctcatc caagaaatta
    11521 aaataaaaat gataaacttt ttgaaaatag gggatttgcc tactttatga tataatggaa
    11581 agagttagat tgaaagaggt agtgagatgg acgcgaaatt aagatacaag gcaaagaaga
    11641 tcaagattgt cttttttgat attgatgata cattgcggaa ttcaaagaca gggtttattc
    11701 caactacaat tcccactgtt tttaaacagt tgcgtgaaaa aggaatttta acaggaatcg
    11761 cctctggacg tggcattttt ggtgttgttc cagagattcg tgatctcaag cctgactttt
    11821 ttgtaacttt gaatggggct tatatcgaag ataaaaaagg tcaggtcatt tatcagcatc
    11881 agattggaaa gtcagatgtt gaggagtata tctcttgggc taagcaagaa ggaattgagt
    11941 atggcttggt tgggagtcat gatgccaagt tgtcgactcg caccgatatg atgagtgaag
    12001 ctatcaatcc aatttatccc gacttagatg tagatcccga tttccatgaa aaagaagata
    12061 tctatcagat gtggactttt gaagataagg gagatgactt gcacttgcct gacagtctct
    12121 cagacaaact tcgcatggtt cgttggcatc aacattcgtc tgatattgtg cctatttcag
    12181 gctccaaagc gacgggggtg gaaaaggttg tggaacacct tggcttgaaa ccagagaagg
    12241 tcatggtttt tggagatggt ctcaatgact tggaactctt tgattatgct ggaatcagcg
    12301 ttgccatggg aatttctcat gataaaatca aagaaaaagc agattatatt acaaaaacat
    12361 tagaagaaga tggcattttt gatgccttag aagtatttgg tatggtagaa aaagaattgc
    12421 attttccaca agtagacatt gaaacagtag aaggtcctct tgcgactatt aagaccaatc
    12481 acggagactt acgtatcaag cttttccctg aacatgctcc taaaacagtg gctaactttg
    12541 tatctctttc aaaagatggc tactatgatg gtgtcatttt ccaccgtatt atcaaggact
    12601 ttatgatcca aggtggagac ccaactggaa ctggtatggg tggcgagtca atctacggcg
    12661 aatcttttga ggatgaattc tcagaagagc tttacaatat ccgtggtgct ctttccatgg
    12721 caaatgctgg cccaaatacc aacggcagcc agttctttat cgtgcaaaac caacacctac
    12781 cttattctaa gaaagaaatt actcgtggtg gttggccaga accgattgca gaaatctatg
    12841 ccaatcaagg tgggacacct cacctagacc gccgtcacac ggtttttggt cagttagctg
    12901 atgaagcatc ttacgctgtc ttggatgcca ttgctgctgt tgagacagga gctatggaca
    12961 agccagttga agatgttgta attgaaacta ttgaaatcga ggactaagat gaaaatcggt
    13021 gataagctaa aggggcgtat tacagggatt cagccctacg gtgcctttgt tgagttagag
    13081 acgggtgata cggggctgat tcacatttca gaaattcgga caggatttat tgaaaatatt
    13141 catgaaacct tgaaagtcga tgaagaggtt caagttcagg tagtggattt agatgaattt
    13201 acggggaaag ccagtctttc tatccgcact ttggaggaag aaaagtacca gtttcctaga
    13261 cggagacgct tttcaagcga ccgctttaac tacggttttg ctccctttcg acgaatgcta
    13321 ccaatctgga ctggagaagc cctgcactat ttaaaaaaga agaagtagtt tagagtttta
    13381 tattttttgt aatgttaata taaatttgct ataataggac catggaagaa gaacaattat
    13441 taaaatcagg agagcgcatt aaccagctct tttcgacaga tatcaaaatc attcaaaata
    13501 gagaggtttt tagctattcg gtggatagtg ttctcttatc acgatttcca cgttttccta
    13561 agaaggggtt gattgtagat ttctgtgctg ggaatggagc agtggggctt tttgctagca
    13621 ctcgtactca agcacagata ttgtctgttg agattcagga gcgtttggcg gatatggctg
    13681 aacgctctgt ccgtttgaat ggattagagg agcaaatgca ggtcatctgc gatgatttga
    13741 aaaatatgcc tgctcacatt caggggagta aggtggatat gattttgtgt aatccaccct
    13801 atttcaaggt ggatcctcat tctaatctga acgagagtga acattatctc ttggctcgac
    13861 acgaaatcac gactaatttg gaagaaatct gtcatagtgc ccagagtatt ctcaagtcta
    13921 atgggcgttt ggccatggtt catcgtcctg atcgacttct ggatattttg gatacgttaa
    13981 aacgacataa tctagcccct aagcgcctgc agtttgttta tccaaaaaga gaaaaggaag
    14041 ccaatatgct tttgattgag gcgatcaagg atggctcaac aagtggcttt aaggtcttac
    14101 ctcctttgat tgtccacaat gatgatggct cttatacgcc cgaaatcgaa gagatttatt
    14161 atggatcata aggcttatat gtacgttctg gagtgtcgtg atggatccta ctatataggc
    14221 tatacgactg atgtgagaag acgcctcgct atccacaata gtgggaaggg agccaaatat
    14281 acacgagcac gcttgccagt caaacttatc tatgctcaag gttttgccag taaggaagaa
    14341 gccatgtcgg ctgaagctct tctcaagcgt aagaagaggc cacagaagga agaattttta
    14401 tctgaaaatc aagatagaaa tttactcagt tattttgaag aatcgtgggg agtcctttga
    14461 ctcctcttac ctttgtcaaa aagcgaaaaa aatgctacaa taaagaaaat aaacaaaatg
    14521 aggtattatc atgtccaaga ttctagtatt tggtcaccaa aatccagact cagatgccat
    14581 cggatcatct gtagcttttg cctaccttgc aaaagaagct tacggtttgg atacggaagc
    14641 tgttgccctt ggaactccaa atgaagaaac agcctttgtc ttgaactatt ttggtgtgga
    14701 agcaccacgt gttatcactt ctgccaaagc agagggggca gagcaagtta tcttgactga
    14761 ccacaatgaa ttccaacaat ctgtatcaga tatcgctgaa gtagaagttt acggtgttgt
    14821 agaccaccac cgtgtggcta actttgaaac tgcaagccca ctttacatgc gtttggagcc
    14881 agttggatca gcgtcttcaa tcgtttaccg tatgttcaaa gaacatggtg tagctgtgcc
    14941 taaagagatt gcaggtttga tgctttcagg tttgatttca gatacccttc ttttgaaatc
    15001 accaacaaca cacccaacag ataaaatcat tgctcctgaa ttggctgaat tggctggtgt
    15061 gaacttggaa gaatatggtt tggcaatgtt gaaagctggt accaacttgg ctagcaaatc
    15121 tgctgaagaa ttgattgaca tcgatgctaa gacttttgaa ctcaacggaa ataatgtccg
    15181 tgttgcccaa gtgaacacag ttgacatcgc tgaagttttg gaacgccaag cagaaattga
    15241 agctgcaatg caagctgcca acgaatcaaa cggctactct gactttgtct tgatgattac
    15301 agatatcgtc aactcaaact cagaaatctt ggctcttggt gccaatatgg acaaggtcga
    15361 agcggctttc aacttcaaac ttgaaaacaa tcatgccttc cttgctggtg ccgtttcacg
    15421 taagaaacaa gtggtacctc aattaactga aagctttaat gcgtaagatt ttgggtgtca
    15481 gctcaaaatc ggaaagtcta gtttgcctta tatcgaaagg agtttcggct cctttttttc
    15541 taggagtgaa gtatgttaga aaatggcgat ttgatttttg tgagagatgg gtcagacatg
    15601 ggacaggcca tccagacttc cacaggtaac tatagccatg ttgccattta tttggatggg
    15661 atgatttatc atgctagtgg acaggctggt gttgtctgtc aagaaccggc agacttcttt
    15721 gagtccaatc atttatacga cctctatgtt tacccagaaa tggatatcca gtcggtgaag
    15781 gaaagagctt gcagacatct tggagcaccc tacaatgctt ctttctatcc agatgcagct
    15841 gctttctact gctcccagta tatagcagaa atcctaccta tttttgaaac tattcctatg
    15901 aaatttggag atggggaaca ggagattagt gatttttgga gggagtatta catagaacta
    15961 ggtctgcctg ttcctctgaa ccaagctggg accaatccta gtcagttggc agcatcgcct
    16021 ctgttacaat gtaaagaaag ggatcttcat gattcagatt tttaatccat ctcgtttgac
    16081 gagacagcca tttttggaga attgatccgc tatctggacc agtatgagga tgtgattcta
    16141 cgggaaatta agtctcagtt tccagatgtt gcagttgata aactcatgga agagtatata
    16201 aaggcaagct tgattctacg tgaaaataag cgctattacc ttaattttcc tacgcttgaa
    16261 tcacttgata gtcttgaact ggatcaagag atttttgtca gagaagctag tccggtctat
    16321 caagccttgt tggagcagag ttttgagacg gaattgcgca atcaaatcaa tgcagctatt
    16381 ttagttgaaa agacggactt tgcgcgcatt aaaatgaccc tgtccaatta tttttacaag
    16441 gtcaaacagc agtatccttt gacagaaaaa cagcaggagc tctatgacat tttaggagat
    16501 gttaatcctg agtatgccct caagtatatg acggcttttt tgttgaaatt tctcaaaaaa
    16561 gaccagctta tgcagaaatg ccgtgatatc tttgtggaca gtttggttgt cttaggctat
    16621 attgtgcaaa atgaagatag aaagtatgag ttggctatcg attttgataa ggagagatta
    16681 actttctact tagcgtgatt tcttgtttct gagcacattg tttgactttc cttagtattc
    16741 ggtataaact atatgtaacc ggtaacacat atcggaataa actaaaggag acaatcatat
    16801 gtcacttgaa aacaaattgg aacaagcaac aggcgctgtc aaagaaggtt ttggtaaagt
    16861 tactggagac agcaagacag aacttgaagg agctgttgaa aaaacagttg ctaaggcaaa
    16921 agacgttgta gaagacgcaa aaggtgctgt agaaggtgcc gttgaaggtt tgaaaaacgt
    16981 ttttactaaa taataggaaa aaatcaaggg tttcattttc ccttgatttt ttctattctt
    17041 ataaataatt ttctgcgacg gctgtatctc ctgggtagga ttctttcttg ccctggatga
    17101 tttggtaaca atcggctccc ttacccgcaa taataactgc atctaattcg tgatttgtga
    17161 tagccattgc cgccttgatg gcttcttggc gatccgcaat cttttcaaca ggatgattga
    17221 tgtagctact aatttcatct gcaatggcca ttgggtcttc atagttaggg tcatcagcag
    17281 tcagaaagac ttgaatctca gggtgttgat tgaggaggag gccaaagtcc ttacgacgac
    17341 tttctccctt gtttcctgtt gatcccagaa ccagagcaat ctttccggtt tgatgagttt
    17401 caaccacatt gatgagtttt ttcagactat ccccattgtg ggcatagtcg atgaagacct
    17461 tggctccatt tttctgagtg aggacttcca tacgaccagg aacgcgggtt gcagcgatgc
    17521 cttttttgat gtcctcaaga cttgctccga gacggagaca agcaagtcca gcagcaactg
    17581 cattttcttg gttgaagttg ccaatgagtt ggatatcata atctccagcg agtttacccg
    17641 tagctgaaaa gctaaaggct ttggaattct cgatttggtt atcaaattgg ctaccataga
    17701 aatcatggtc ttgatcttca acctgttctt tcaagactga gaagtggtcc atgtcactgt
    17761 taatgatgac tgctcggcta ttttccatca agagacgctt gtggtagaaa tagtcttcaa
    17821 agctagggtg ttcaatcggg ccgatatggt caggagtgat gttaagaaag actcctacat
    17881 caaaggtcag tccatagact cgatggacta gataggcttg gctagagact tccattacga
    17941 gatggcttct tccattctta acagcttgag ccatcatgtc aaagaggtcg atattttcag
    18001 gtgttgagaa tgaagattta aagaaagttg tgccatccaa agttgtattc atggttgata
    18061 agagggcggt tgggtaacgt tgagatagga tgttgtaagc aaagtaagct gctgttgtct
    18121 tacccttagt tcctgtgaag gcgagaattt tgagtttctc ttgtggatta ccatagaact
    18181 ccatggcaat caaactcatg gctttcttta tatcgttcac aatgatgaca gggataccga
    18241 cttcgtagtc cttttcagct acataccaag ctaaaccttg tgttatagca gaaagaaggt
    18301 attctttttt aaaggcagca ccttttgcaa aaaaaagagt gtcttctgtt acttttcggc
    18361 tgtcgtagct gatgctatca aaaacaactt tgctgtagtt gtagtggtaa tgaccttggt
    18421 caataatttc gcgaaaaagg ccatctttct ttaaaatatc taatacagtt tcaattttta
    18481 tcatactttc tattgtaaac cgaaagtcgc aaatttacaa gtaacaagga aaagtttata
    18541 atggaagata aggagttttt cctagttatc aaaattgaat gaggaatcta tgtcgcacga
    18601 aaacaatcac cagcaggccc agatgttacg ggggactgct tggctaacgg ctagtaactt
    18661 tatcagtcgc ctactcgggg ctgtttacat tatcccttgg tacatctgga tgggggctta
    18721 tgcagctaag gcaaatggtc tctttaccat gggttacaat atctatgctt ggttcttgtt
    18781 ggtttcaaca gcggggattc cagttgcggt ggccaagcaa gttgccaagt ataataccat
    18841 gcgagaagaa gagcatagct ttgccctgat tcggagcttc ttaggcttta tgacaggact
    18901 aggcctggtt tttgctttag tcttgtatgt ctttgctcct tggctagcag acttgtctgg
    18961 cgtgggcaaa gacttgatcc caatcatgca aagcttggct tggggagtct tgattttccc
    19021 gtctatgagt gttatccgag gatttttcca agggatgaat aacctcaaac cttatgccat
    19081 gagccaaatt gctgagcagg tcattcgtgt tatctggatg ctcctagcaa cctttatcat
    19141 tatgaagctc ggttcaggag attatctagc agccgttacc caatcaacct ttgctgcctt
    19201 tgtcggtatg gtagccagtt ttgcagtctt gatttatttc cttgcccaag aaggttcact
    19261 caaaagagtc tttgaaacag gagataagat taacagtaag cgtctcttgg ttgataccat
    19321 taaggaagcc attcctttta tcctgacagg gtctgccatc cagctcttcc agattttgga
    19381 tcagctgacc tttatcaata gtatgagctg gtttaccaac tacagcaatg aggacttggt
    19441 tgtcatgttt tcttatttct cagccaatcc taataaaatc acgatgattt tgatttctgt
    19501 aggggtttcg attgggagtg ttggtttgcc acttttgacg gaaaactatg tcaaggggga
    19561 cttgaaagcg gcttctcgtc tcgttcagga cagtctcacc ctactcttta tgttcttgct
    19621 accagcaacg gttggagtgg ttatggtagg agaacctctt tatacggtct tctatggtaa
    19681 gccagatagt ttggctctgg gcttatttgt ctttgcagtt ttgcagtcta ttattttagg
    19741 cttgtacatg gtcttgtctc caatgcttca ggccatgttc cgcaaccgca aggccgttct
    19801 ctattttatc tatggttcta ttgccaagct agtcttgcaa ctacctacca tcgccctctt
    19861 ccacagttat ggtcctttga tttcaacaac cattgctctc atcattccta acgtcttgat
    19921 gtatcgggat atttgtaaag taactggtgt caagcgcaag gtgattttga agcgaaccat
    19981 tttaatcagt ttgctgaccc tagtcatgtt tctgttaata ggaaccatcc agtggctgtt
    20041 aggatttttc ttccaaccaa gtggacgttt gtggagcttc ttttatgtag ctcttgtcgg
    20101 tgccatgggg ggtggacttt atatggttat gagtctgcgt acctatttat tagataaggt
    20161 aataggaaaa gcccaagcag atcgcctgcg agcaaaattt aagctttcgt aaggtagcat
    20221 ttataaataa aaggaataag ttgaacaatc ttttaggaga acttgaaact tattcctttt
    20281 ttagcgtcaa aatagctaaa attatctttc aaaaatagaa aatgagtttt ttgatttttt
    20341 ggaaaaaaca attgactaaa caaaccaaga gttgtataat aaaacaaata tttaaagcag
    20401 gaggttctgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg ttttgtcctg
    20461 ggtacaggag ttttattgag cgcgtgtgga aattctagca ctgcatcaaa aacctataac
    20521 tatgtttatt caagtgatcc atctagtttg aactatctag cagaaaaccg cgcaacaacc
    20581 aatgatattg tgaccaatct ggtagatggt ctcatggaaa atgaccaata tgggaactat
    20641 gtgccatcct tggcagaaga ttggactgtt tctcaggatg gtttgaccta tacctacaaa
    20701 cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcacc tgtaactgcc
    20761 caggattttg tgacaggatt gcaatatgca gctgataaaa aatcagaagc cttgtatcta
    20821 gtgcaggact ctgttgcagg tttggatgac tatatcactg gtaaaacaag cgacttttca
    20881 actgtcggta tcaaggctct tgatgatcaa accgttcaat acaccttggc tcgaccagaa
    20941 ccttactgga actcaaaaac aacctcaacc attcttttcc cagtcaatgc agatttcttg
    21001 aaatcaaaag gggatgattt tggcaaggta gaccctgcta atattttgta cagtggacct
    21061 ttcttgatga aagcttttgt ttcaaaatct gtcatcgaat acaagaaaaa tcctaactac
    21121 tgggatgcta aaaatgtctt tgtagacgat gtgaaattga cctactatga tggtagtgac
    21181 caagatgctc ttgttcgtaa ctttacagac ggagcctata gctatgcccg tctctatccg
    21241 aatagttcaa gttttgaagg gattaaagaa aaatacaaag atgatatcat ctacagtatg
    21301 caggatgcta cgtcttatta ctggaatttt aacctagata gacaagctta taaatttact
    21361 tctaagacga ccgacattga aaagaaatca actcaggaag cagttcttaa taaaaacttc
    21421 cgtcaagcta ttaactttgc ctatgatcgt acctcctatg gggctcaaac tcagggagaa
    21481 gacggagcga ctaagatttt gcgtaacttg gttgttcctc caacctttgt aagtatcaaa
    21541 ggaaaagatt ttggtgaagt agtagcctct aagatggtca ataattatgg taaggaatgg
    21601 caaggcatca actttgcgga tggtcaagac ccttactaca atcctgagaa agccaaggct
    21661 aaatttgctg aggccaagaa agagctagaa gctaagggag ttcaattccc tattcatttg
    21721 gatgcgacag ttgaccaagc ttctaagaag gggattcaag aagtaagctc catgaagcaa
    21781 tctattgaag cagctttagg tacggagaac gtcgttattg atatccaaca gttatcaaca
    21841 gaggactatg ataactcaag ttaccttgct cagactgcta ttcagaaaga ctacgacctt
    21901 tacaatggtg gttggagtcc tgactactta gatccatcaa cctatcttga tatctttagt
    21961 gtcaagaatg gtggggttct acaaaatctt ggactcgacc ctggtgaagc caatgacaag
    22021 gctaaggcag ttggacttga cacttatact caaatgttgg aagaggctaa taaggagcaa
    22081 gacccagcca aacgttatga gaaatatgct gatattcaag catggttgat agatagttct
    22141 ctagcgattc caaatgtttc attaggtgga acgcctagct tgagaaaaac agttcctttc
    22201 tcagcacctt attcattggc tggtaataaa ggggttgaat catataaata cctcaaagta
    22261 caagataaga ttgtcacaac agacgaatat gcaaaagcca gagaaaaatg gttgaaagaa
    22321 aaagaagaat ccaataaaaa agcccaagaa gaattggcaa aacatgtcaa ataaaatcta
    22381 attgtagata agtttgtgta agaagtcttt
//
LOCUS       DAVHIU010000021        22398 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVHIU010000021 DAVHIU010000000
VERSION     DAVHIU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.022"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(293..2551)
                     /gene="glgP"
                     /locus_tag="VVN06_000686"
     CDS             complement(293..2551)
                     /gene="glgP"
                     /locus_tag="VVN06_000686"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU5562282.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2577..4094)
                     /gene="malQ"
                     /locus_tag="VVN06_000687"
     CDS             complement(2577..4094)
                     /gene="malQ"
                     /locus_tag="VVN06_000687"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU5562283.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4644..5915
                     /locus_tag="VVN06_000688"
     CDS             4644..5915
                     /locus_tag="VVN06_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5562284.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGTKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6026..7318
                     /locus_tag="VVN06_000689"
     CDS             6026..7318
                     /locus_tag="VVN06_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5562285.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7320..8162
                     /locus_tag="VVN06_000690"
     CDS             7320..8162
                     /locus_tag="VVN06_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5562286.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8415..9215
                     /locus_tag="VVN06_000691"
     CDS             8415..9215
                     /locus_tag="VVN06_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU5562287.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     SSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9225..10211
                     /locus_tag="VVN06_000692"
     CDS             9225..10211
                     /locus_tag="VVN06_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5562288.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10278..10460
                     /locus_tag="VVN06_000693"
     CDS             10278..10460
                     /locus_tag="VVN06_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562289.1"
                     /translation="MRFNVGRFTSFSTEIESFPKIFLYSMKIKVQTRKLAAGCSKHCF
                     EVADRTDEVSNIYTAR"
     gene            complement(10502..11443)
                     /locus_tag="VVN06_000694"
     CDS             complement(10502..11443)
                     /locus_tag="VVN06_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU5562290.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11421..13184)
                     /gene="aspS"
                     /locus_tag="VVN06_000695"
     CDS             complement(11421..13184)
                     /gene="aspS"
                     /locus_tag="VVN06_000695"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5562291.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(13420..13500)
                     /locus_tag="VVN06_000696"
                     /pseudo
     CDS             complement(13420..13500)
                     /locus_tag="VVN06_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(13536..13766)
                     /locus_tag="VVN06_000697"
     CDS             complement(13536..13766)
                     /locus_tag="VVN06_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562292.1"
                     /translation="MKRVILVAVIQAVVLFFIIGGLAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(13784..14425)
                     /locus_tag="VVN06_000698"
     CDS             complement(13784..14425)
                     /locus_tag="VVN06_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562293.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLVLGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14437..15171)
                     /locus_tag="VVN06_000699"
     CDS             complement(14437..15171)
                     /locus_tag="VVN06_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU5562294.1"
                     /translation="MKKKRRLLFLMSIVFGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(15174..15371)
                     /locus_tag="VVN06_000700"
     CDS             complement(15174..15371)
                     /locus_tag="VVN06_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562295.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(15371..15577)
                     /locus_tag="VVN06_000701"
     CDS             complement(15371..15577)
                     /locus_tag="VVN06_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5562296.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15587..15901)
                     /locus_tag="VVN06_000702"
     CDS             complement(15587..15901)
                     /locus_tag="VVN06_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562297.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(16123..16203)
                     /locus_tag="VVN06_000703"
                     /pseudo
     CDS             complement(16123..16203)
                     /locus_tag="VVN06_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(16194..17483)
                     /locus_tag="VVN06_000704"
     CDS             complement(16194..17483)
                     /locus_tag="VVN06_000704"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5562298.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(17561..18808)
                     /locus_tag="VVN06_000705"
     CDS             complement(17561..18808)
                     /locus_tag="VVN06_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU5562299.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFWKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTVCFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(18999..19850)
                     /gene="rgg"
                     /locus_tag="VVN06_000706"
     CDS             complement(18999..19850)
                     /gene="rgg"
                     /locus_tag="VVN06_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU5562300.1"
                     /translation="MEQIGKVFRQLRESRNISLRQVTGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            19974..20315
                     /locus_tag="VVN06_000707"
     CDS             19974..20315
                     /locus_tag="VVN06_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5562301.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            20373..20453
                     /locus_tag="VVN06_000708"
                     /pseudo
     CDS             20373..20453
                     /locus_tag="VVN06_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20569..22272)
                     /gene="ilvD"
                     /locus_tag="VVN06_000709"
     CDS             complement(20569..22272)
                     /gene="ilvD"
                     /locus_tag="VVN06_000709"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5562302.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT         6725 a         4903 c         4276 g         6494 t
ORIGIN      
        1 ggtaatcaac tacgaaaaaa tataaggctg aggacagtaa aaaccaacat ttaaagcctt
       61 cactgtatct aggatttccc tatttttaga caagattgac ccaaatctac atcattaaaa
      121 aagcgaattt caatcgaaac tcgctttttt acaagatgta ttagtataaa tttgtatctt
      181 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagcaccg
      241 ctttgaggtt gcagataaag ctgacgtagt ttgaagagat ttttgaagag tattagttca
      301 agtgccagat gtcttcgtta tactgagcga ttgtacggtc agatgagaag aatcctgctt
      361 tagaaatgtt aacgatgact ttatccaacc atgcgtcacg gtcttcgtag tcagcaagca
      421 tttgctcttt gactttgatg tagtcttcca agtcaagaag agtcatgaac cagtctttgt
      481 tgatcaattc attgtaaaga cgttccaagc gctctttgtt tccagctgca agaactgcat
      541 cactaacgat gaagtcaacc aatggtttga tagcttcacg agcgtagaat tcgcttgatt
      601 tgtaagctgc ttttgcgtaa aggtcgataa cagtttctga atcttcaccg aagatgtaga
      661 tgttttcttc tccaaccaac tcagcgattt ccacgttagc accgtccata gtaccaagtg
      721 tcaaagctcc gttcaacatg aatttcatgt taccagtacc tgaagcttct ttagaagcaa
      781 gtgagatttg ttctgagata tcacatgctg ggataaggaa gcttgctgca gtaacgttgt
      841 agttttcaac cataactact tgcaagtgtg gagctactgc tggatcgtta gcaataactt
      901 ctgacatgca aaggatcaag tgaatgatat cttgagcgat tgtgtaggct ggagctgctt
      961 taccaccaaa gaagattgtg attggacgag cagggatgtt accagctttg atgtcaaggt
     1021 atttgtggat cacgtacaaa gcgttcattt gttggcgttt gtactcgtga agacgtttga
     1081 tttggatatc aaagatagaa tttggattga tttccacacc ttggtgttct ttcaagtgac
     1141 gagccaattt acgtttgttg tgagccttga tgctttccaa tttttctttg acagctgctt
     1201 tgtcttcata agacaacagt ttttcaagct catctgcttc atggtgccaa ccatctccaa
     1261 gaatctcatc caagtagtga gacaatcttg ggttagcatg cataagccaa cgacggaaag
     1321 tgataccgtt tgttttgttg ttgaactttt ctgggtaaag gtcgtagaag gctttcaact
     1381 cagaattttt caagatttca gtatggagtg ctgcaacccc gttaacactg tatccgtagt
     1441 ggatatccat gtgagccatg tgaacacgtc cgctctcatc gatgatttga acagctggat
     1501 ctttgtactc tgccttcaca cgacggtcca attcttcgat gattggtacc aagtgaggaa
     1561 ccacttcttg caagaattca agaggccatt tttcaagcgc ctcagcaagg attgtgtggt
     1621 tagtgtaggc agtcatgcta cgaacaattg agattgcttc gtcaagctcg ataccacgtg
     1681 cagtcaaaag acgaatcaat tcaggaatca ccattgatgg gtgagtatcg ttgatttgga
     1741 caactgcgta gtcagcaagg tcatgcaagt tgcttccttt ttcgattgct tcgtcgatga
     1801 tcaattgcgc accgtttgaa accatgaagt attgttggaa gatacggagc aattcacctt
     1861 gacggtcact atcatctggg taaaggaaga gagttaagtt gcgagcgata tctgtcttgt
     1921 caaagttgat accatcttta ataatagaag aatcaactga atccaagtca aacaaacgca
     1981 agcggttttt agtcgctgtt tcataaccag taacatcaat atcgtaaaga gttgatgtca
     2041 aagtaaagtc tgcaaatggt acttggtagc tacggcttga gcgaaccaac cagttttgct
     2101 ctgtcaacca tgcatttgga attgtttctt gttggttgtt tttaagaact tgttggaaaa
     2161 gaccaaagtg gtagttaaga ccaacaccgt caccattcaa accaagagta gcaattgagt
     2221 cgataaagca ggcagccaaa cgtcccaaac caccattacc aagagatggt tccaattcaa
     2281 cttcttcaac ttcgatcaag tctttacctg cagctgcaag ttctttttta acatcgtcgt
     2341 aaagaccaag gttaatcaag ttgtttgaca agagtttacc aatcaagaac tcagctgaga
     2401 tgtagtaaac ttttttctta ccagtgttga ctggtttttg gctgcttgca agcttgctgt
     2461 agttaagaag agcaaggtaa agctcttcat tgctacattc tgcaatggtt ttattgtaac
     2521 gattttgtac aaattcttgt agtgataaca tgtttagttg tctcctgatt attgtcttat
     2581 ttctttaaat ctaccaaatt ttcattaatt cggcgataaa ttgttgtcaa gtcaagcaaa
     2641 ccttcctcga cagctggtgt caattgatct tcagtcatac gccaagacca gtttccacca
     2701 agggtagatg ggaagttcat acgagctgcc tcatccaatt ctagtaaatc ttgcatagtt
     2761 gcaattgcca taaagctaac tgatgaaaat actgtacgaa gcatagcgtg taccactgtt
     2821 tcgtattctt tacggttcgt gtaacgagcc atgtactcac gagtcgcatc atcaatctca
     2881 ttacggtacc aaccaagaac cgtattgtta tcgtgtgttc ctgtgtacat aactgagtta
     2941 gcaggtgcca agtgtgggct atcaatactt tcgtcttctg ggttgaaggc aaattgaaga
     3001 atcttcattc ctgggaagcc agtacgttca cgcaattcga tcacttcatc tgtcatgaag
     3061 ccaaggtctt ctgcgatgat gtttagctca ccaagttctt ccttaacggc tgcaaaaagc
     3121 ttgtagcctg gacctttcac ccactcacca ggtgctgctg tatcggaacc agcagggatt
     3181 tcccaataag attcgaagcc acggaagtgg tcgatacgaa cgatatcgta gattttgaag
     3241 ctttcacgca agcgttcaat ccaccatttg tagccgtctt tgtccattgc ttcccagtca
     3301 tagattgggt taccccaaag ctgaccagtt acagaaaact catctggtgg gcatcctgcg
     3361 atacaagtag ccttaccatt gacatctgtt ttgaagagat gtggatttgc ccacatatca
     3421 cttgaatctt ccgctacgta gattggcatg tccccaacga tttcgatgtg gttgtcgtta
     3481 gcgtaagctt tcaatttcaa ccattgttgg aagaagaagt attgagtcac acggtggtaa
     3541 accaacttgt ctgccaattg ctcacgatag ctttcaagtg ctgaagcttt acgagcacga
     3601 gcatctgcat ctggccattc agtccaagca agattgtcaa aatactcttt gatagccata
     3661 tactcagcaa agagctcaag ccatgattgg ttgtcttgag caaatttctc aaaatcttta
     3721 acatctccga cttcaaagaa acgtttcacc gctttttcta aaagaggacg acgtgcatag
     3781 tagattttag catagtcaac ttcagacgca tcgctaccaa agtcaactcc ttcaaggtca
     3841 cttgcttcca acaaaccttg ctccaccaag atatctaaat cgataaaatg agtgtttcct
     3901 gcgaaggctg agaaagattg gtaaggagaa tccccgtaac tagttgctcc taatggaagg
     3961 atttgccagt aacgttgttt tgtacggacc aagaaatcaa cgaagtcgta agcactttga
     4021 ccaaatgatc cgattccgta agctcctgga agagaagaga tgtgcatcaa cacaccactt
     4081 tgacgttttt tcataatagc acctcgtgtg ttaaaataat ggaacgttgc gtatttttca
     4141 gacgcaaacg tttgcgttac ttataagtat actccttttc aagcataatt gcaagcgttt
     4201 ttaaaaaact tttttgatta tttttaagga gactaactca ttttagcaaa gataacttaa
     4261 aactatctaa tcctaaggtt tgactctcac gagacagtct acaaagtaca aaacctcctt
     4321 agggagcttg aatttatatg caacaaagca caaacgtcta tagaaaatag atagggatta
     4381 gaaccaggga ggtagcccct cctgatttcc ctctttagac agattccata tatttttatt
     4441 aaaaatactg tattcttatc tatttcatac ttttcttcta ttaaaatgca aaattatgct
     4501 atcaaatcaa gcttaaaaat tttttaaaat ttttacaaaa aatacttgca accgttttct
     4561 atttgtgcta tactaagctc ataaaggaaa acgtttgcgt ttccttatca ataaaacttg
     4621 ctattctttg ggaggaatac actatgtcat ctaaatttat gaagagcact gcggtgcttg
     4681 gaactgttac acttgctagc ttgcttttgg tagcttgcgg aagcaaaact gctgataagc
     4741 ctgctgattc tggttcatct gaagtcaaag aactcactgt atatgtagac gagggatata
     4801 agagctatat tgaagaggtt gctaaagctt atgaaaaaga agctggagta aaagtcactc
     4861 ttaaaactgg tgatgctcta ggaggtcttg ataaactttc tcttgacaac caatctggta
     4921 atgtccctga tgttatgatg gctccatacg accgtgtagg tagccttggt tctgacggac
     4981 aactttcaga agtgaaattg agcgatggta ctaaaacaga cgacacaact aaatctcttg
     5041 taacagctgc taatggtaaa gtttacggtg ctcctgccgt tatcgagtca cttgttatgt
     5101 actacaacaa agacttggtg aaagatgctc caaaaacatt tgctgacttg gaaaaccttg
     5161 ctaaagatag caaatacgca ttcgctggtg aagatggtaa aactactgcc ttcctagctg
     5221 actggacaaa cttctactat acatatggac ttcttgccgg taacggtgct tacgtctttg
     5281 gccaaaacgg taaagacgct aaagacatcg gtcttgcaaa cgacggttct atcgtaggta
     5341 tcaattacgc taaatcttgg tacgaaaaat ggcctaaagg tatgcaagat acagaaggtg
     5401 ctggaaactt aatccaaact caattccaag aaggtaaaac agctgctatc atcgacggac
     5461 cttggaaagc tcaagccttt aaagatgcta aagtaaacta cggagttgca actatcccaa
     5521 ctcttccaaa tggaaaagaa tatgctgcat tcggtggtgg taaagcttgg gtcattcctc
     5581 aagccgttaa gaaccttgaa gcttctcaaa aatttgtaga cttccttgtt gcaactgaac
     5641 aacaaaaagt attatatgat aagactaacg aaatcccagc taatactgag gctcgttcat
     5701 acgctgaagg taaaaacgat gagttgacaa cagctgttat caaacagttc aagaacactc
     5761 aaccactgcc aaacatctct caaatgtctg cagtttggga tccagcgaaa aatatgctct
     5821 ttgatgctgt aagtggtcaa aaagatgcta aaacagctgc taacgatgct gtaacattga
     5881 tcaaagaaac aatcaaacaa aaatttggtg aataaaaaat ttgttcaagg ggggtggaaa
     5941 tcaaatcccc ctttgaattt atcaatagag acacaaataa tttagctttc ttataaaaaa
     6001 gtagtatcct atgaaaggag ttaatatgga aaagcaacaa cctagtaaag cagccctgct
     6061 gtctatcatt cctgggttag gacagattta caataaacaa aaagccaaag gttttatctt
     6121 ccttggtgta accatcgtat ttgtccttta cttcctagca cttgcaaccc ctgaattgag
     6181 caacctcatc actcttggtg acaaaccagg tcgtgataat tccctcttta tgctgattcg
     6241 tggtgccttc catctaatct ttgtaatcgt ttatgtactc ttttatttct caaatatcaa
     6301 agatgcacat acgattgcaa aacgcattaa caatggaatt ccagttccac gcacactcaa
     6361 agacatgatc aaagggattt atgaaaatgg cttcccttac ctcttgatca ttccatctta
     6421 tgttgccatg accttcgcga ttatcttccc agttatcgta accttgatga tcgcctttac
     6481 caactacgac ttccaacact tgccaccaaa caagttgttg gactgggttg gtttgaccaa
     6541 ctttacaaac atttggagct tgagtacctt ccgttctgcc tttggttctg ttctttcttg
     6601 gactatcatt tgggctttgg cagcttctac tttacaaatc gtaattggta tcttcacagc
     6661 tatcattgcc aaccaaccat ttatcaaagg aaaacgtatc tttggtgtta ttttccttct
     6721 tccttgggct gtcccagcct tcatcactat cttgacattc tcaaacatgt ttaacgatag
     6781 tgtcggtgct atcaacactc aagtattgcc aatcttggct aaattccttc ctttccttga
     6841 tggagctctt attccttgga aaacagaccc aacttggact aagattgcct tgattatgat
     6901 gcaaggttgg ctcggattcc catacatcta cgttttgact ttgggtatct tgcaatctat
     6961 tcctaacgac ctttacgaag cagcttatat tgacggtgcc aacgcttggc aaaaattccg
     7021 caacatcact ttcccaatga ttttggctgt tgcggcacct actttgatta gccaatacac
     7081 cttcaacttt aacaacttct ctatcatgta cctcttcaat ggtggaggac ctggtagtgt
     7141 cggaggtgga gctggttcaa ccgatatctt gatctcatgg atctaccgtt tgacaacagg
     7201 tacatctcct caatactcaa tggcggcagc tgttaccttg attatctcta tcattgtcat
     7261 ctcaatctct atgatcgcat tcaagaaact acacgcattt gatatggagg acgtctaaga
     7321 tgaataactc aattaaactc aaacgtagac tgactcaaag ccttacttac ctttacctga
     7381 ttggtctatc aattgtaatt atctatccac tgttgattac cattatgtca gcctttaaag
     7441 caggtaacgt ctcagccttt aaactagata ctaatatcga cctcaatttt gataacttta
     7501 aaggcctctt cactgaaacc ttgtacggta cttggtacct caacactttg attatcgcct
     7561 taattaccat ggctgttcaa acaagtatca tcgtacttgc tggttatgct tacagccgtt
     7621 acaacttctt ggctcgtaaa caaagtttgg tcttcttctt gatcatccaa atggtgccaa
     7681 ctatggccgc tttgacagcc ttcttcgtta tggcgcttat gttgaacgcc cttaaccaca
     7741 actggttcct catcttcctc tacgttggtg gtggtatccc tatgaatgct tggctcatga
     7801 aaggctactt cgatacagtg ccaatgtctt tagacgaatc tgcaaaacta gacggtgcag
     7861 gacacttccg ccgcttctgg caaattgttc ttccacttgt tcgcccaatg gttgccgtac
     7921 aagctctctg ggccttcatg ggacctttcg gggactacat cctctctagt ttcttgcttc
     7981 gtgagaaaga atactttact gttgccgtag gtctccaaac cttcgttaac aatgcgaaaa
     8041 acttgaagat tgcctacttc tcagcaggtg ctatcctcat cgcccttcca atctgtattc
     8101 tcttcttctt cctacaaaag aactttgttt caggacttac aagtggtggc gacaagggat
     8161 aatttatccc cgccaccctt tttcatttta tactcttcga aaatctcttc aaaccacgtc
     8221 agctttatct ccaacctcaa agttgtgctt tgagcaacct gtggctagtt tgcactttga
     8281 ttttcattga ttattagcaa ttgtcactgt aaataatatc cttgtagcaa gcaatttttc
     8341 tcctagactt gaaataaagc gcatttctct atataataat actcatatag aaaacacctt
     8401 ttagaaagat acctatgctt ccatatccat tttcctattt ttcaagtatt tgggggtttc
     8461 gtaagcccct gtccaaacgt ttcgagctca actggtttca acttctcttt accagtatct
     8521 tccttatcag cttgtctatg gtacccattg ctatccaaaa cagctcccag gagacctatc
     8581 cgctagaaac ttttatcgat aatgtctatg aacctctgac aaatgaggtt gtccaggatc
     8641 tctctgaaca tgctacaatt gtcgatggca cattaactta tactggaaca gttagtcaag
     8701 cctcttctgt tgtgattggt tcaagtcaaa tcaaggaatt acctaagaac ttgcaactgc
     8761 atttcgatac aaatgagcta gtcatcagca aggaaagcaa ggaactgacc cgcatctctt
     8821 accgagccat tcagactgag agtttcaaaa gcaaagacag cttgacccaa gcaatttcta
     8881 aagactggta ccaacaaaat cgtgtctata tcagcctctt cctagttctc ggtgcgagct
     8941 tcctctttgg tttgaatttc tttatcgtct ctctaggagc tagtcttctc ctttatatca
     9001 ctaagaaatc acgcctcttt tcatttagaa cctttaaaga gtgctatcac tttatcttga
     9061 actgtttagg attgccgact ctgattacac ttattttggg attatttggc caaaatatga
     9121 caaccctgat tactgtacaa aatattcttt ttgttctgta tctggtcact atcttttata
     9181 aaacacattt ccgtgatcca aattaccata aataggagat ttttatgccc gttacgatta
     9241 aagacgtggc caaggctgct ggtgtttcgc cttcaaccgt aacccgtgtt attcaaaata
     9301 aatcaaccat tagcgacgaa acaaaaaaac gcgttcgcaa agctatgaag gaactcaact
     9361 accacccaaa cctcaacgct cgtagcttgg taagcagcta tactcaggtt atcggattag
     9421 ttcttcctga tgactcagac gccttctacc agaatccttt ctttccatcg gttctacgtg
     9481 gcatctctca agtcgcatct gaaaaccact atgccattca gatagcaaca gggaaagatg
     9541 agaaggagcg tctcaacgct atttcacaaa tggtctacgg caagcgtgta gatgggctaa
     9601 tctttctcta tgcccaagaa gaagaccctc tcgtaaaact cgtcgcagaa gaacagttcc
     9661 ccttccttat cttaggtaaa tctctatctc ctttcatccc acttgtcgac aacgacaatg
     9721 ttcaagctgg ttttgatgcg actgaatatt tcatcaaaaa aggctgcaaa cgcattgcct
     9781 ttatcggagg aagtaaaaag ctcttcgtga ccaaagaccg tttaacaggc tatgaacagg
     9841 cgcttaaaca ttacaaactt accactgaca acaatcgcat ctactttgcc gacgagtttc
     9901 tggaagaaaa gggctataaa tttagcaagc gattattcaa gcacgatcca caaattgatg
     9961 ctatcatcac aaccgatagc ctcctagctg aaggtgtttg taactatatt gccaaacacc
    10021 agctggatgt ccctgttctc agctttgact cggttaatcc caaactcaac ttggcagcct
    10081 atgtcgatat caatagttta gagcttggtc gtgtttccct tgaaactatt ctccagatta
    10141 ttaatgataa taaaaacaat aaacaaattt gttaccgtca attgatcgcc cacaaaatta
    10201 tcgaaaaata agagactggg caaaaagtcg ttaaaagcaa aaacgcatag tatcaggtat
    10261 tgaaaaaact tgatactatg cgttttaatg tgggaagatt tacttccttt tctactgaaa
    10321 ttgagtcttt tcccaagatc tttttatact caatgaaaat caaagtgcaa actaggaagc
    10381 tagccgcagg ttgctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagta
    10441 acatatatac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtattaatc
    10501 actaattatc tatctcaaca aatcttccta gaatatgaac attttccgag acagagacaa
    10561 aggagcttgg atccacttgt gtcataatct gtttaaattc attaaactct gcacgtgtaa
    10621 tgacagtgat taaaactgcc tttctctcgt gattataggt tccttctgca tcgtggatca
    10681 tggttgctcc gcggtgcaat tttttatgga ttttttcaat taccttctct ggatgatttg
    10741 tcacaatcat ggcctgcata cgcttttgct tagtaaagac tgcgtctgtc acacggctag
    10801 agacaaagat ggtaatcata gaataaagag cgtatttcca accaaaggtc aaacctgcta
    10861 tcagcatgat agttccattt accaagaaag aaatactacc gacattctta cccgttttct
    10921 tgcgaatagt caggctgacg atatccgtcc caccactgga gatattgttt cgaagagcaa
    10981 aaccaatccc caaacccata acaacacccc caaaaaggga attgataatg ggatcctccg
    11041 tcaaggttgc cacagggaca aactggataa agaaggaact catagatacc gtgataaagg
    11101 taaagacggt gaacttatgg ccaatctgat accaagctaa gaccatcaaa gggaagttaa
    11161 tggcgtagaa gcttagcgaa atcggaatat gaaaaccaaa ccagtgatta ctcaaggcag
    11221 agataatctg tgccagacct gttgcaccac tcgaatacac atgccctggt tggaaaaaga
    11281 aattaactgc tactgctgat aaaaaaccat agaccagaga ggccgaaatc ttctcatcat
    11341 acttttctcg agagatactt tgtaagacac gtaaaatttt tatctgataa gcaaagcggc
    11401 gcagataata gcgccaccgc ttaattcgtt ttgcttgttt catcttcttc tacttgtaag
    11461 ctgagttcct ctagttgttt gagagcgact gttgatggag cttgtgtcat tgggtcagtt
    11521 gccttgttgt tcttaggaaa ggcaatgact tcacggatat tttcttctcc agcaagcaac
    11581 atgacaaaac ggtcaagccc gatagccaaa ccaccgtgtg gtgggaaacc atagtccatg
    11641 gcttcaagaa ggaaaccaaa ctggtcattg gcttcttcag ctgagaaacc aagagccttg
    11701 aacatgcgtt cttgaaggtc tttttggttg atacgaaggc taccaccacc aagctcataa
    11761 ccgttcaaga cgatatcgta agcaatggca cgaaccttag ccaaatcacc ttctaattcg
    11821 tgagcagtct cttcctgtgg aagggtgaaa ggatggtggg cgctcatgta gcggccttct
    11881 tcttcagacc attcaaacat cggccagtca accacccaaa ggaagttgaa cttatcatta
    11941 tcaatcaagc caagctcttt agcaatacgt ccacgaaggg cacccagtgt tgcattagcc
    12001 acttcaagcg tatccgccac aaagagaacc aagtccttat cttcaagagc aagcgctgtt
    12061 gtcaattctt cttggatacc agtcaagaac ttggcaactg gtccgtttaa ttctccatca
    12121 accaccttaa cccaagcaag acctttggca ccatactgtt tggctacttc cgtcatcttg
    12181 tcgatgtctt tacgtgaata gttgtccgca gctcctttga ccacaatcgc ttttacagca
    12241 ggtgcttctg aaaagacttt aaagtctaca cctttgacca cttctgtcaa gtcctgaagc
    12301 aacatgtcaa aacgagtatc tggcttgtca gaaccgtaaa gagccatagc atcatcgtat
    12361 ttcacacgag ggaacggtag cgttacttcg atgccttttg tttccttcat cacgcgcgcg
    12421 atcaagcctt ctgtaatatc ttggatttct tgctcagtaa ggaaggacgt ttccaagtcg
    12481 acctgagtaa attcaggctg gcggtctcca cgcaagtctt cgtcacggaa acatttaacg
    12541 atttggtagt aacggtcaaa accagcattc atcaagagct gtttcgtgat ttgtggactt
    12601 tgaggaagag cgtaaaaatg ccccttatta acacgagacg gcactaaata atcacgcgcc
    12661 ccttcaggcg ttgacttaga aaggaatggt gtctccacgt cgataaactc caactcatcc
    12721 aagtagttgc ggatagagtg ggtcaccttg gcacgaagtt taagattttc caacatttct
    12781 ggacgacgaa ggtcaaggta acggtaacgc aaacgtgtat cgtcatttgc ctcaatgcca
    12841 tccttaatct caaatggtgt tgtcttagct gtgttaagca caataagagc tgtcacgttt
    12901 aactcaaccg caccagttgg caacttatca ttggcttgct cacgcgcagc gacctgacca
    12961 gtcacctcaa taacaaattc gctacgaagg ctttcagctg ttgccataac ctctgcagat
    13021 actttttcag ggttgataac caactgcatg attccttcac ggtcacgaag atcgataaag
    13081 atcaaaccac caaggtcacg acgacggcca acccatcctt tcaaggttat ttcttgtccg
    13141 atgtgttcct cacgaacacg accagcatac atactacgtt tcattatttc tctcctcttt
    13201 tattctgtta ctattttacc ataaaagcgc aggctcttca tgaaaatcat cagaaaagtt
    13261 tgccagtctt taaaagtcag atgaaagccc taaaaattag cgctaatact cttcgaaaat
    13321 ctcttcaaac cacgtcagcg tcgccttacc gtaggtatgg ttactgactc cgtcagtttc
    13381 atctacaacc tcaaaaccat gttttgagct gacttcgtca gtttcatcca caacctcaaa
    13441 acagtgtttt gagcaacctg cggttagctt cctagtttgc tctttgattt tcattgagta
    13501 taatacaaaa atccgatgaa cttcaccgga ctcttttatt ttgaattttt gcctgcttta
    13561 cgcttttcag cgatttcggc tgcctttcga ggcaagacaa tttccgttat gtaagccgtc
    13621 ccaaaacgca gtacacctgc aataggagca aagacaactg ctagatagtt atagaagaaa
    13681 tcgcctttga aggcataagc tagccctcca atgatgaaaa atagaacgac tgcctgaatc
    13741 actgctacta aaattactcg tttcatgtga cctcctgact ctattatagc atgagaatca
    13801 tcaaaaagcc gactaaatta ttcaaagcgt gaagagaaat actgtagacc agaccttttc
    13861 tgctaatgta agccaaaccc aaactaaaac caaggctaaa atagacaaaa aattgttgca
    13921 catcacctgg aaaatgaatc aaggcaaata gaagactaga taccagaaga aaaatcaggg
    13981 ttcgtttact attgtcctgc ttaggaaaga gatagcgtgc taacatccct ctaaaaacaa
    14041 tctcttccgt caaaggagca aaaataacca cagcaaagaa tgagaaaagt ggttgagaca
    14101 aggtcaagtc tgtcgctatt tgctgattta ctgaaggatc atctggcaag aagaattgaa
    14161 cgaccagaga taagaaccaa accaaggcag gaagccaaat aaatcggtta aagccgctct
    14221 tctcaatata aacaggagct ttctgatacc atttgtaaat gccgtacaca tatactccag
    14281 ccaagaccac atagagtaga gtaacagcat agggtgaagc gcctaaaaca agcgacgcag
    14341 tcgcgagccc ctgaataaag ccatagataa agaaaaagga tagaagggct agaagaaacc
    14401 agccaaggtt tttaagtagt ttcatagata actcctttat ttgaaataac gttttaccat
    14461 aggtaactgc atcacattga tataaacatg gatggctcct acaagcaaga aagctagtaa
    14521 ctgaatctct cctgtcaaga aagaaatgat aataagaaaa atatataagg ctggtaagac
    14581 atattggtgt aattggaata aaattcgaaa actctgttcc aaattagcct gacgctcccc
    14641 ttcatcataa gaatttatat agttcaagac atcctttggt gtagcgaaaa attccaaatc
    14701 aaactgacga acaatcgcaa tggttttaaa aagagatttt tgagcgacta agaataccac
    14761 aaagagtaag aaagaaagga aaaatgtttg agggtttgta tgcaatataa tcacctcact
    14821 taatgaaata aaaatagcca atggaatcgc tacacctgta atattaaaag caatggttcc
    14881 aaactcaaga ttccgataca tttgcacata ataggtttca ttcagatcgt catccatttc
    14941 ctcttgatac aaggaatgaa attttctact tttctttaag aaattgaaag tcaaaaacat
    15001 actaatgaaa cctatcagta aacaaatagc tgatatccat ggcatcaagg cttttacatc
    15061 taaaataatt tcgtgggatt cgacacgtgc cttaaacatc cctacaaaca tgcccaagaa
    15121 ccccccaaag acaatagaca tcaaaaataa caatctacgt ttctttttca tattcattct
    15181 cctttttcac ttgctagatt tttggatttc ttttcaatcc attcaattac tgggatgaga
    15241 gcaaagtaga cccaaacaaa ttggtcgctt tgatagggat taaaccagct taggtccatc
    15301 ccaatcagta gaaatacgct gactaataaa gctatgacca ctacataata aatcacttta
    15361 tacttgttca tcactcgtcc tcctccaaac gaaataccga ttcgactgtt tcgttgaaaa
    15421 tttgagatat tttcagggca atgataatgg atggggtgta ctcatcccgt tctagtaggc
    15481 taatggtctg tctggaaacc cctgccagtt tggctaggtc ggtttgattg agaccatcgc
    15541 gagctcgaag ctcttttaga cgatttttta gttgcatgtt acacacctac tctccgtcaa
    15601 attcaacggt ttggatatcc tcaatacgtt gcaacttgaa tttttctttt cccgtattat
    15661 ctacacgtcg tagctttacc cattcctcat caacatccac aacttcccag ttatctggcc
    15721 caatatacac tcccgttata attggttcct ttccaatcat ttcttgtaat aatctcgaca
    15781 tttctgcatt tcctttctct tttcgctcaa gtcttttgat tttcttctct agtttcttga
    15841 tttttttaga atcattagaa taaaagaaaa tcagaaatag tataaaccct agtacccaca
    15901 tcatagctcc tttcttcttt tctcttaact tgcttacatt gtaacacatc tttttctttt
    15961 tgacaagtat agttgtcaaa aagtttatga tttttgtcat tttgcaaaag aaaaaggtca
    16021 ggagtaagtt cctgaccact ttatctatca ttaatactct tctaaaatat cttcaaacca
    16081 cgtcagcttc accttgccgt aggtatggtt actgacttcg tcagtttcat ctacaacctc
    16141 aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
    16201 gtataaaatc ctagtttttc aaagatttct gagaagtttt ggctgattgt ctcaagtgac
    16261 acttgcactt cttctcgggt ttggttgttc ttgaccgtca cttgtccgct ttcgacttcg
    16321 ctctctccta gggtgatgag ggtcttagcc gcaaagacat ctgctgactt gaactgagct
    16381 ttgagcttac ggttgaggta atcacgctct gctttgaaac cttgttggcg aagagcttgg
    16441 actagttcta gggctttgac atttgctcca tcacccaaga ctgcgatata gacatctagg
    16501 gcgttttcga tagggagggc cacgccttgt ttttcaagga tgagaagcag gcgctctaca
    16561 ccaagcccaa aaccaaatcc agcagtttca gggcctccaa agtaagcaac caaaccatcg
    16621 tagcgaccac ccgcacagac tgtcaagtca ttgccctcaa tctctgtgat aaactcgaaa
    16681 atggtgtggt tgtagtagtc cagaccacgc accatattgg tatcgatgat gtaatctact
    16741 ccaagatttt ccaacatctg acgcacagca tcaaaatgag tttggctttc ttcatcaaga
    16801 aagtccaaga tagacggcgc attctctact gccaccttgt cttctttttc cttagagtcc
    16861 aagacacgaa gaggattttc ctccaagcga cgttggctat ccttagacaa ggtctccttg
    16921 agcggtgtca aatagtcaat caaggcttgg cggtaggctg cacggctctc aggatttcca
    16981 agagtgttga ggtgcaattt gacaccttga ataccgattt ccttcaaaaa atgggctgcc
    17041 ataacgattg tttccacatc ggtagctgga ttgctagagc caaaacactc aacaccaatc
    17101 tggtggaatt ggcgcaagcg ccctgcctgt ggacgctcat aacggaacat aggtcccatg
    17161 tagtagaact tgcttggctt ttgcacttct ggggcgaaga gtttattttc cacataggaa
    17221 cggacaacgg gcgcagttcc ttctggacgg agggtaatat ggcggtcacc cttgtcatag
    17281 aaatcgtaca tttccttggt tacgatatcc gttgtatctc cgacagagcg gctgataacc
    17341 tcgtaatgct cgaaaatagg cgtacgcact tctgcgtagt tgtagcgctt gaaaatttca
    17401 cgggcaaagc cctcaacgta ctgccactta gcagactcag caggtaaaat atcctgcgtt
    17461 ccttttggtt tttgtaattt catagggaat cctctttaaa cttaatagtc ttattttacc
    17521 ataaatagag ggattaaaac agtgagaaaa aaattaggat ttagatatca tttttgagat
    17581 taagaattgt caaaaaaata gctagcaagg aaaaaccaac aaatagcatc caagtcaact
    17641 gtatattcca tacggctact agtgaaaaac aaactgttcc cacaggtatg gataaggtaa
    17701 acaatagacc taaaaaatta ctagtacgag ctagaacctc tggagctaga tttttcatga
    17761 gcatggcact aatctttggt tgaactttac cagacacata cagagtaaag aagagaaata
    17821 gcaaaccaag cacgacttga ttgaataaat tagccaaacc aactagacta agtcctacgg
    17881 tcgcccacat catcaatcta ggcaaggact gcttccaaaa ataatcattg cccgtaaggc
    17941 tactgatgat gattgctact aaaacacaga attgattgat aaatagtgcc tctgtataag
    18001 aaaaattcaa gagagaatgg ctcaaaaaga agatattata aattccaccc aaagcgccac
    18061 ccaaggaatt aataagcaag acagcaaaga gcataaaacc aaagtttttc tgtccacttt
    18121 taagaaaaac gagacgtaaa tttcggtaaa ttgttaggaa ctggtctttg atagaaagct
    18181 tctcattttt taagttttca ccatcagcag atgacattga caggctcaat ttgctttttc
    18241 ctaaaaagag gatagtggct gatactagga aaaagcaggc attgattccc gcaacgaggg
    18301 aaaaattgtt gaccgataga gctaagagcc agactccgaa agcttgacca ccaatagctg
    18361 aaatataggt gatgaactgt gaaaaagaat aagcctccat caaatcatct tcagctactt
    18421 tttccttaat aagaggcata cgcaggccac ctgcaaaatc actgatgata tcactaatga
    18481 cattgatcaa acacaggcta gaaaaggcaa agagactagc ttgctgaaca actagggctg
    18541 ctagaaaaaa tagaaccgcc tgaaacaaac cgctatagac catccatttg accttgtccc
    18601 tcgtgtaatc tgcccgaatc cctgcaaaaa ctgtaaagaa ggtcggaaga atcatgacaa
    18661 tattcgccat agcaacagca aaagatgctt gtgacaaggt cgatgcatag acgataaaga
    18721 ccaggttgaa aatcgaagca ccaaaagcat tgaagaagcg tgataaagtc aaaattctat
    18781 aagctggatt tctaaacaat agtttcatag ataatctccc tccttgttct aagttctaca
    18841 tcaagaatag ctttagtata gcacttatga atttgcgaaa cgattctttt gaatatatgc
    18901 aacaaacaaa atgagcaaaa aaacgataga acttttgtaa tgacctgaga gagggcaaca
    18961 gttctatcgc ttcaaatttt ttcttggttt gcagatattc aacattcggt cgtatttcta
    19021 tagtattcgg cagatttatt acagccaagc atctcaaaaa tacggacagc atcctccatc
    19081 tttttctggc cttccttgac tctaccttgc ttgctatcaa ggagaccttc tgcccacaga
    19141 taaacaattc ggaaataggt ctcattttcc ttgtagaaat gctcttcgat aacacgttta
    19201 aaataatagg cattggtaaa ttcttcacac tcaatactag ctaaaaagcc attcaatagt
    19261 atagtatgaa aaaggtttcg attgccagac atttccatta gaaaatcaga tttacgtacc
    19321 atttctcgta catatctagt aaaaagagaa acagataaaa atggagaact gactgaaaat
    19381 aaattgagtt catagattcc ccagatctcg gtagaaaaca aataatcatg aaggactttt
    19441 ccttcctctg ctgttaactc taccctttca tctatgctct tcatataaga cttgataata
    19501 atggcattta gaatatgttt ctgtttgttg tgagaatggg catgcttttg atactccctg
    19561 cgatataagt cctcaagagg tgctatattc tttggttcca agacatctgt aatttctttt
    19621 ctcaactcag aatctgtatc atactggaaa cctctcgcca gaaagaggat ctcctccaca
    19681 ctggcagata tattttccag agcaaataga aacttttcca ccgaaagctc actctgacct
    19741 gtttcaaaac gggacaacat agacggcgaa aattgtcctc cggttacttg tctcagtgag
    19801 atatttcttg actctcgtaa ttgtctaaag acttttccaa tctgctccat agacttcccc
    19861 ttgattccgt attttcttca ttttatcata tttttcagaa aattcatcaa aaacttgcca
    19921 aattgtcaga attatgagaa aatagaggat atttatcacg tggagggact gctatgagag
    19981 acgatatcaa aatcaatgac cgtgctttgg ccttgcaaga ccaaatcatc gaaaaactag
    20041 agaaagtttt tgatacagat gtggaattgg atgtttacaa tctaggtctg atttatgaaa
    20101 tcaatctgga tgaaacgggg ctctgcaaga ttgtcatgac cttcaccgat actgcctgtg
    20161 attgcgccga aagcctgcct attgaaatcg tggcaggtct gaaacaaatc gagggtatca
    20221 aggatatcaa ggttgaagtt acctggtcgc ctgcttggaa aatcacacga atcagtcgct
    20281 atggccgtat tgcccttgga ctaccacctc gttaagcagg ccaatcactt ttaaagatga
    20341 aaaaagcaag ccgaaaatgg cttgcttttt gatactcaat gaaaatcaaa gggcaaacta
    20401 gaaaactagc cgcaggttgc tcaaaacact gttttgaagt tatggataga actgacgaag
    20461 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccatacct
    20521 acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt attttttacc
    20581 tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg atacgatgtg
    20641 ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt ctgccttgcg
    20701 tttttcaagt tcttcttcgg atacggccat ggaaatttct ttggtatctt ggtcaaccgt
    20761 aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt caggagcgat
    20821 atgtccaaca acaagaccat aagtaccacc agagaaacgt ccgtccgtca agagggccac
    20881 cttatctccc tgacctttac caacaatcat tgaagaaagt gacagcatct caggcatacc
    20941 aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa cgatttcatc
    21001 tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg gcccaacgtg
    21061 acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa ggttcccgtt
    21121 caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga taactttttg
    21181 gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac cagtacatgt
    21241 gatgcgatct ccgtgaagga aaccatttgc caacaagtac ttcataaccg cagggacacc
    21301 accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt cggccaagtg
    21361 aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat ttgcggcatg
    21421 ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca tcgttacagt
    21481 aatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac caagttccaa
    21541 catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag ctgattcagc
    21601 tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag ttgccatggt
    21661 attagcagta tacataccac cacaaccacc agggccaggg caggcattac attcaagacg
    21721 tttcacgtcc tcagctgtca tgtcaccgtg gttccatttt ccgatacctt caaagacaga
    21781 aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac cataggcgaa
    21841 aatagctggg atatccatat tagcaatagc aatcatagat ccaggcatgt tcttgtcaca
    21901 gccaccgata gcgacgaagg catcgacgtt gtgaccactc atagccgcct cgatggagtc
    21961 cgcgatgatg tcacgagatg ttagagagaa acgcatacca ggcgttccca tagcgatccc
    22021 gtctgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt tgacaccttc
    22081 tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt ccgcccaagt
    22141 cgaaatcact cctacaatcg atgtttcaaa gtccttatct gtcataccag tcgcacgaag
    22201 catagcacgg ttaggtgatt taaccatgct gtcataaatg ctactgcggt gacgtttatc
    22261 taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca atttaaaggc
    22321 aatgaacaga ataaaattct tgaattttca gaaaattcta tacgcatgtg aaatatttaa
    22381 aattaaaaac aacaaagc
//
LOCUS       DAVHIU010000022        21637 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVHIU010000022 DAVHIU010000000
VERSION     DAVHIU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.024"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            5..75
                     /locus_tag="VVN06_000710"
     tRNA            5..75
                     /locus_tag="VVN06_000710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VVN06_000711"
     tRNA            110..183
                     /locus_tag="VVN06_000711"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VVN06_000712"
     tRNA            266..355
                     /locus_tag="VVN06_000712"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VVN06_000713"
     tRNA            366..439
                     /locus_tag="VVN06_000713"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VVN06_000714"
     tRNA            443..515
                     /locus_tag="VVN06_000714"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VVN06_000715"
     tRNA            528..608
                     /locus_tag="VVN06_000715"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VVN06_000716"
     tRNA            613..683
                     /locus_tag="VVN06_000716"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VVN06_000717"
     tRNA            698..770
                     /locus_tag="VVN06_000717"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VVN06_000718"
     tRNA            781..852
                     /locus_tag="VVN06_000718"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VVN06_000719"
     tRNA            862..945
                     /locus_tag="VVN06_000719"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VVN06_000720"
     CDS             1052..2329
                     /locus_tag="VVN06_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU5562303.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYFFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VVN06_000721"
     CDS             2405..3889
                     /locus_tag="VVN06_000721"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5562304.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTVELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEISPIRHKTTVAAADMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VVN06_000722"
     CDS             3950..5230
                     /locus_tag="VVN06_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5562305.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5234..5854
                     /locus_tag="VVN06_000723"
     CDS             5234..5854
                     /locus_tag="VVN06_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5562306.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSDFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7162
                     /locus_tag="VVN06_000724"
     CDS             6020..7162
                     /locus_tag="VVN06_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5562307.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVIVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7485..7910
                     /locus_tag="VVN06_000725"
     CDS             7485..7910
                     /locus_tag="VVN06_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU5562308.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7921..8322
                     /locus_tag="VVN06_000726"
     CDS             7921..8322
                     /locus_tag="VVN06_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU5562309.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            8397..9041
                     /gene="pcp"
                     /locus_tag="VVN06_000727"
     CDS             8397..9041
                     /gene="pcp"
                     /locus_tag="VVN06_000727"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU5562310.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9069..9926)
                     /locus_tag="VVN06_000728"
     CDS             complement(9069..9926)
                     /locus_tag="VVN06_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU5562311.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10056..11198
                     /gene="tgt"
                     /locus_tag="VVN06_000729"
     CDS             10056..11198
                     /gene="tgt"
                     /locus_tag="VVN06_000729"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5562312.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11299..13116
                     /locus_tag="VVN06_000730"
     CDS             11299..13116
                     /locus_tag="VVN06_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU5562313.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGNYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQSRSN
                     GQLRGMVFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSILATIVGV
                     RQTTSLVKQLDKIWDLRKTLLVFAGGFGFLLILTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTPNVQEPKMISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPVLQTLRPLPYIHAILATSTGILVFIVFLVTLLAPQV
                     GAFETDLTVNGLKQAASNIAQTKVMAERAEADSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSRTTKTANEIMLNNSQNKFLPKMVVIATGVNNPENYKEDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITVADWNQVAKEHPEIWAG
                     TDQVHFGSDTSTIEAGAKLYAETIATALQTAQDKPVKSK"
     gene            13269..14420
                     /gene="nagA"
                     /locus_tag="VVN06_000731"
     CDS             13269..14420
                     /gene="nagA"
                     /locus_tag="VVN06_000731"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU5562314.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14583..15641
                     /locus_tag="VVN06_000732"
     CDS             14583..15641
                     /locus_tag="VVN06_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5562315.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15792..16157
                     /locus_tag="VVN06_000733"
     CDS             15792..16157
                     /locus_tag="VVN06_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU5562316.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16233..17174
                     /locus_tag="VVN06_000734"
     CDS             16233..17174
                     /locus_tag="VVN06_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU5562317.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17122..18138
                     /locus_tag="VVN06_000735"
     CDS             17122..18138
                     /locus_tag="VVN06_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5562318.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18140..18466
                     /gene="comGC"
                     /locus_tag="VVN06_000736"
     CDS             18140..18466
                     /gene="comGC"
                     /locus_tag="VVN06_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU5562319.1"
                     /translation="MKKMMTFLKKSKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVA
                     NRKVND"
     gene            18459..18863
                     /locus_tag="VVN06_000737"
     CDS             18459..18863
                     /locus_tag="VVN06_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU5562320.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18868..19128
                     /locus_tag="VVN06_000738"
     CDS             18868..19128
                     /locus_tag="VVN06_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562321.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19091..19552
                     /locus_tag="VVN06_000739"
     CDS             19091..19552
                     /locus_tag="VVN06_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU5562322.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19530..19943
                     /locus_tag="VVN06_000740"
     CDS             19530..19943
                     /locus_tag="VVN06_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU5562323.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            20080..20667
                     /locus_tag="VVN06_000741"
     CDS             20080..20667
                     /locus_tag="VVN06_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5562324.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVNSQILYSAEDLFQGNYSEFFLIVAQKSLA"
     gene            20728..>21637
                     /locus_tag="VVN06_000742"
     CDS             20728..>21637
                     /locus_tag="VVN06_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5562325.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMK"
BASE COUNT         6350 a         3886 c         5030 g         6371 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtagggtag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttataaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttct ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaagc
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgcctgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattatttc cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagact taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgctagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggtta cttatccaaa
     2521 ggtagatttg gactttgaca aattgaaaga tacttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagc tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcaaca tctggtgaca cggggaaagc tgctatggcg gggtttgcgg atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaagga tggtgtcagc aagattcaag agttacaaat
     2941 gaccactcaa actggcgaca atactcatgt tattgctatt gatggtaact ttgacgatgc
     3001 gcaaacaaat gtgaagcaca tgtttaacga cgtggctctt cgtgaaaaat tgactaccaa
     3061 caagttgcaa ttttcatcag ctaactctat gaacattggt cgtctggtgc cacaaattgt
     3121 ttattatgtt tatgcttacg ctcaattggt caagactggt gagatcgtgg ctggtgataa
     3181 gattaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaatcggt ctcccagttg gtaaattgat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttc aagacacgtg tttacgacaa gaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttgggaaa
     3421 taatgctgaa aagacagttg aacttatgaa tgccttgaac acgcaaggac aatataagtt
     3481 gacagacttt gatgcagaga ttttggacct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacggcagca gagatcaagc gtgtttgtga gttagattct tatatcgagg accctcatac
     3601 agctgttgct tcagcagttt ataaaaaata ccaatcggcc actggagatg taactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga aatatctcca attcgtcaca agacaacagt
     3841 ggcagctgct gacatgcaag cagcggttga ggcttattta ggactttaag acagagggag
     3901 caaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 gggagccttg attcgtgcaa cgcataatcc acgtttgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttctgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagtcgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttggaacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggcatg tggtgtatcc gcattggaac
     5101 aggatatctg ctggggattg tgcttggttg gggcttgcct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgagctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtgactt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagt atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg tacagtatgg
     6181 tgataccttg agcactattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg atcgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccaattgc agaagctcca aaagaagtag catcaagttc
     6481 agaagttaca aagacagtga ttgcttctga agaagtggca ccatctacgg gcacttctgt
     6541 cccagaggag caaacggccg aaacaagcag tgcagttgca gaagaagctc ctcaggaaac
     6601 gactccagct gagaagcagg aaacacaagc aagccctcaa gctgcatcag cagtggaagt
     6661 aactacaaca agttcagaag caaaagaagt agcatcatca aatggagcta cagcagcagt
     6721 ttctacttat caaccagaag agacgaaaat aatttcaaca acttacgagg ctccagctgc
     6781 gcccgattat gctggacttg cagtagcaaa atctgaaaat gcaggtcttc aaccacaaac
     6841 agctgccttt aaagaagaaa ttgctaactt gtttggcatt acatccttta gtggttatcg
     6901 tccaggagac agtggagatc acggaaaagg tttggctatc gactttatgg taccagaacg
     6961 ttcagaatta ggggataaga ttgcggaata tgctattcaa aatatggcca gccgtggcat
     7021 tagttacatc atctggaaac aacgtttcta tgctccattc gatagcaaat atgggccagc
     7081 taacacttgg aacccaatgc cagaccgtgg tagtgtgaca gaaaatcact atgatcacgt
     7141 tcacgtttca atgaatggat aaacccgact tgataacatc attttgacga atgagatcta
     7201 gctttcgtga tggcaagcga ttctcgttcg ttttttcttt gtcatactct tcgaaaatct
     7261 cttcaaacca cgtcagtttt atctgaaact tcaaagctgt gctttgagca acctgcgact
     7321 agcttcctag tttgcttttt gattttcatt gagtatcaat ttgaatggaa aatggaaagt
     7381 tatcatcttg taatgagtta agcaacattc ttgcaatcta ttttacttta tatcacaatt
     7441 aattagtcaa atattgataa atcaataaaa agagagggga agaaatgcta gagattcaag
     7501 atttactgta tcaactccgc ttgtctgagc aagcgagtac gcaattgttt gaaaaaaggc
     7561 ttgggattag tttgacacgg tatcagattt tactgttttt gctggagcat tctccttgta
     7621 accaaatggc gattcaggag cgtttgaaaa ttgatcaggc tgctttgaca cggcatttca
     7681 agattttgga aacggaaggt ttggtggagc gtcatcgtaa tcctgaaaat cagcgggaag
     7741 tgttggtaga ggctgcgaag tatgccaagg agcagttagt ggtgaatccc cctctgcaac
     7801 atatcaaggt taaggaagag atggaaagta tcttaacaga gtttgagaga acagaactca
     7861 gccgtttatt aaataaattg gttttgggta ttgaaaatat agaaatttaa ggagaaatag
     7921 atgtcaatta ttacaattat tttaacaacg atcgttgctt tggagcattt ttacattttt
     7981 tatttggaaa gtattgctac gcgatcagat gcgactagtc gtgtatttaa tatggaaaag
     8041 gaagaattgg ctcatccgtc agtaagttca ttgttcaaaa atcaagggat ttataaggct
     8101 ctgctaggag tctttctctg gtatggcatt tatttctcac agaatttaga aattgtgact
     8161 atttttgtct tatttgtgat tggtgctgcg acttacggct ctttaacagc ggataaaaaa
     8221 attattttga agcaaggtgg atcagctatt ttggccttga ttagtatttt actctttaaa
     8281 atacacttga aggtcgattc taatctcgct aatccttttt aatccagaat aagggaaata
     8341 tgttatactt gtttttaaga aaaaagtctc attgaattgg ttttgaggag ttagaaatga
     8401 aagtattagt gacaggtttt gagccctttg gaggggaaaa gggcaatcca actttggagg
     8461 ccattaaagg tttaccagct gaaatccatg gtgctgaggt ccgttggcta gaggtgccga
     8521 cagtttttca caaatctgct caagtattgg aagaagagat gaatcgttat caacctgact
     8581 ttgtcctttg tattgggcaa gctggtggaa gaactagtgt gacacctgaa cgagtggcca
     8641 ttaatcaaga cgatgcacgc atttctgata acgaagataa tcaaccgatt gaccgtccca
     8701 ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc gattaaagcg atggttcaag
     8761 ctataaaaaa agagggctta ccggcctctg tttccaatac ggcagggact tttgtctgca
     8821 gccatttgat gtatcaggct ctctatttgg tagaaaagaa atttccatat gttaaggcag
     8881 gttttatgca tattccttat atgatggaac aggtggtgaa cagaccgact actccaacta
     8941 tgagtttagt ggatattcgg cgagggatag aagcagcaat cggcgctatg atagaacatg
     9001 gagatcagga actcaagttg gtaggcggag aaactcattg atagaaaaaa gcttgagggg
     9061 aaaaaccttc aagcttttgg acgttttcga gccaatactg ctcggtaaaa cataatttta
     9121 gtgcattgga tataaggtag gagtgaaaaa ctagcaatgc caaaggtaat ccaattgagg
     9181 aagtaccaag gaagaagctg taaatctagg acaaagtgct ggaacttgta gcccttcata
     9241 aaggaacggc tagtttttag gattcgtctt ggtgggacct gtcctaggtc tagactataa
     9301 cagagaagaa attccacctg tgaataggca taatactgtg gaatatagag gatatttcct
     9361 acaatgatca agatgagact tgcaagaaag tagagtccaa agaccatgag gaaacgctcg
     9421 gtttcaactg atgagagatc tagatttgga aactcaggat gtagggtgac gaattttttg
     9481 gctaaaaagc tactataaaa gaggagataa atcccaagta aattagggat actccataaa
     9541 aagagataga aacgtttgag aagtagggtc aaaaaggttt gagaaaagcg ctcctcatca
     9601 aagagagcta ggctgttttt tacagatggc tccgttttag aatctttcat gagtgtcagt
     9661 gttgcataga cggaactggt caaaagaata gtcccgataa aggagactag tagaggaaag
     9721 aggtaggctt gaagtatttg gccaagtatg ctgaaaaatg gctgttctaa aacagtcccg
     9781 tggatccgag ataagggatt aagaaaacta gataagatga ccagcatact gggaaggata
     9841 tagaggagaa agagacgggg ggtgtcagcc tgaaaatgtt ttgactcctg acgaattgtt
     9901 tttaaatcaa tttttggata gttcattctc ttattatacc atagttctta tacatagttc
     9961 gtgacagttc ctactttttt tgataaaatc atacagtgtg tccttgggca cactgtatga
    10021 actgggactg tctttcccag cttcggaggt aaaaaatgtc agattcacca atcaaatatc
    10081 gtttgattaa gaaagaaaaa cacacaggag ctcgtctagg agaaatcatc actccccacg
    10141 gtacctttcc gacacctatg tttatgccag ttgggacaca agccactgtc aaaactcagt
    10201 cacctgaaga attgaaggag atgggttcgg gaattatcct atcaaacact tatcatctct
    10261 ggcttcgccc tggagatgaa ctcattgcac gcgctggtgg tctccacaag ttcatgaatt
    10321 gggaccagcc tatcttgaca gatagtggtg gttttcaggt ttattcttta gcagatagcc
    10381 gtaatatcac agaagaagga gtaaccttta aaaatcatct aaatggttct aagatgttcc
    10441 tatccccaga aaaagccatc tctattcaga ataatctggg ttcagacatc atgatgtcct
    10501 ttgatgaatg tcctcagttt tatcaacctt atgactacgt taagaaatcg atcgagcgta
    10561 ccagccgttg ggctgagcgt ggtttgaagg ctcaccgtcg tccacatgac caaggtttgt
    10621 ttggaattgt gcaaggtgca ggatttgaag accttcgccg ccaatcagct catgatcttg
    10681 tcagcatgga tttctcaggc tactctatcg gtggtttggc agtgggagaa acccatgaag
    10741 agatgaatgc ggtcttggac tttacaactc aactgctgcc tgaaaataaa cctcgttatc
    10801 tgatgggtgt gggagcgcca gatagcttga tcgatggggt cattcgtggg gtggatatgt
    10861 ttgactgtgt cttaccgact cgaattgctc gtaacgggac ttgtatgacc agtcaaggac
    10921 gtttggttgt gaaaaatgcc cagtttgctg aggactttac gccactggat cctgagtgtg
    10981 actgctacac atgtaataac tatacacgcg cttaccttcg tcacctgctc aaggctgatg
    11041 aaacctttgg tatccgcttg actagctacc acaatcttta cttcttgctt aacctgatga
    11101 agcaagtgcg acaagccatc atggatgaca atctcttgga attccgtgag tattttgtgg
    11161 aaaaatatgg ctataataag tcaggacgca atttctaaaa cggaatagat ataagctaaa
    11221 aatcctaagt tttctcttgg gatttttctt ctttttttga tagaataaag tgtataatga
    11281 aagggagaat aaactcgtat gcgcattaaa tggttttcct tgattaggat tacaggttta
    11341 ctactggtac tcttgtatca tttctttcag accatctttc ctggaggatt tttcggggta
    11401 gatgtctttt tcacattttc aggtttcctg attacggctc tactcatcga agaattttct
    11461 aaaaatcatg agattgactt gataggattt tttaggagac gcttttatcg gattgtgcca
    11521 cctgtggttt tgatggtctt ggtgaccatg ccctttacct ttctagttcg gcaagattat
    11581 gtggctggaa ttgggggtca gattgcgggt gtcttaggct ttatgaccaa cttctatgaa
    11641 cttctaacag gtgggaatta tgaatctcag ttcattccac atttgtttgt tcataattgg
    11701 agcttggctg ttgaggttca ctactatatt ctatggggat tggcagtttg gttcttatcc
    11761 aagcagtcta gatcaaatgg tcagttgaga gggatggtct ttctcttatc tgctactgcc
    11821 ttcttgatta gcttcttctc catgtttatc ggtagttttc tagtgacctc ttattcttct
    11881 gtttatttct ccagtttaac tcatgtctat ccattctttt tgggaagtat cctagcaacg
    11941 attgtaggcg ttcgtcagac gacttcccta gttaagcaat tggataaaat ctgggattta
    12001 cgcaagaccc ttttagtttt tgcaggaggt tttggcttct tactcatttt gaccttcttc
    12061 gtcaaattta cctatctctt tgcctatctt attggcttct tgcttgccag tcttgcagcc
    12121 cttgctatga ttctggcggc gcgtgtctta catgaaaaga cacctaacgt gcaggagcca
    12181 aagatgatta gctttttagc agatactagc tatgcagttt atctcttcca ttggcctttc
    12241 tatatcattt tctcacagtt gacatcaaat cttcttgctg tattactgac tctgatttgt
    12301 tcttatggct ttgccagtct ttcattttat gtgttggaac cttggattgc aggaaagaac
    12361 acacctgttt tacaaactct tcgtcccctg ccttatattc acgcaattct tgcaacaagt
    12421 acaggaatct tggttttcat tgttttctta gtgactttat tggcaccaca agtgggagcg
    12481 tttgagacag acttaactgt caatggcttg aagcaagctg ctagtaatat tgcccaaaca
    12541 aaagtgatgg cagaacgagc agaagctgat agtttgggga ttgctgatgg tactatgtta
    12601 attggtgatt cagtggcttt aagggcaaat acagccctgc agacagctct tcctggagca
    12661 caaattaacg cgcaggtcag cagaacaacc aagactgcca atgaaatcat gctaaacaat
    12721 agccagaata aatttttacc taagatggtg gtcattgcga ctggggtaaa taatcctgaa
    12781 aattacaagg aagactggga cagtattgta aaaaatcttc ctaagggaca ccatatgatt
    12841 ttagtcactc cttatgaggg ggataagaca aaagagacct atgccatcgt tgagaaggct
    12901 gctgcctata tgagagaatt ggcagagaag acaccttata tcacagttgc tgattggaat
    12961 caagttgcga aagagcatcc agaaatttgg gcaggaacag accaagtcca tttcggaagt
    13021 gacactagca ctattgaagc aggagcaaaa ttgtatgcag agaccattgc cacagccttg
    13081 caaacagctc aagacaagcc ggttaaatca aaataacttg tattaaaaaa gagaagagtt
    13141 ggagaaatcc agctctttta aaatatctcc aaaaaatagg tctataccat ttacaaataa
    13201 aaaagaaagg tttataatgt aattgacata ataaattgta gaatcaatct tttaaggagg
    13261 ttaacattat gcctaactat attaaagcgg atcagttttt ctacccacac ggagttcgtc
    13321 gaggtggtta cttggaactt gtggacggca agtttgggaa acatgtagag cagattcctg
    13381 aaggggctga ggtgattgac tatacaggtt atagcattgc cccaggtctt gtggatactc
    13441 atattcatgg atatgcaggt gtagatgtga tggacaacaa cattgaaggt acattgcata
    13501 ctatgagtga aggacttctt agtaccggtg ttaccagttt cttacccaca actttaacag
    13561 ccacttatga gcaattgctt gcagtcactg aaaatcttgg aaaccattat aaagaagcaa
    13621 caggtgctaa gattcgtggg atttattatg aaggtccata tttcacagaa acttttaagg
    13681 gggcacaaaa tccaacttat atgagagacc cgggtgttga ggagtttcat tcttggcaaa
    13741 aagcggcaaa tggcttgctt aataaaattg cccttgcacc agaacgtgat ggggtggaag
    13801 actttgttcg tacagttacg ggcgaaggtg tgacggttgc tcttggacat tcaaacgcga
    13861 cttttgatga agccaaaaaa gcagtcgatg ctggagcgag tgtttgggtg catgcctaca
    13921 atggaatgcg tgggttgact caccgtgaat tgggtatggt tggagccatg taccaattgc
    13981 cacataccta tgcagagttg atctgtgatg gtcaccacgt agatccaaag gcttgcgaaa
    14041 ttcttatcaa acaaaaagga acagaaaaca tcgctcttat cacagactgt atgacagctg
    14101 ggggattgga agacggagat tatatgttgg gagaattccc agttgtcgtt gcaaatggaa
    14161 ctgcacgcct caaatcgaca ggtaacttgg caggttctat cctcaaactc aaagatggtt
    14221 tgaagaatgt ggtcgaatgg ggaattgcga atccgcatga agcagtcatg atggccagct
    14281 tcaacccagc taaatccgtt cacatcgatg acgtctgtgg ccaaatccgt gaaggctacg
    14341 acgctgactt catcgtatta gataaagatt tggaattggt agcaacctat ctagatggcg
    14401 taaaacgtta tcaagcataa gagagaaggc agaagttaga gactagcttc tgctttttta
    14461 tacagggtac tttactggta aataaaaagt taaaaaaatc acaaaaaaag cttgaagaaa
    14521 caaagaaaaa ggcttacaat tgaactataa atattacaaa taaaaaaacg gaggagtgct
    14581 ttatgaaagc ctatacttat gttaaaccag gacttgcttc ttttgttgat gtagacaaac
    14641 cagttattcg caagccaaca gacgctattg tgcgtattgt aaaaaccact atttgtggaa
    14701 cagacctcca tattatcaaa ggggatgttc ctacttgcca aagtggtacc attcttggcc
    14761 acgaagggat tgggattgtt gaagaagttg gggaaggagt ttccaacttc aaaaaaggtg
    14821 acaaggtctt gatttcttgc gtctgtgcct gtggtaaatg ctactactgt aaaaaaggaa
    14881 tttatgctca ctgtgaagac gaagggggct ggattttcgg tcacttgatt gatggtatgc
    14941 aggctgaata tctacgtgtc cctcatgcag ataatactct ttaccatact ccagaagact
    15001 tgtcagatga agctttggtt atgctgtcag acattctgcc tactggatat gaaattggtg
    15061 tcttaaaagg gaaagtagaa cctggttgca gcgtagccat tattggttca ggtccagttg
    15121 gattggctgc tcttttaaca gcccaattct attcaccagc taaattgatt atggtagacc
    15181 tagacgataa ccgcttggaa actgccctat cattcggtgc gactcataag gttaattctt
    15241 cagaccctga aaaagccatt aaagaaattt atgatttgac agatggtcgt ggtgtggatg
    15301 tcgctatcga agctgttggt attcctgcaa catttgattt ctgtcaaaag attatcggtg
    15361 tagacggaac ggttgccaac tgtggtgtgc atggtaaacc agttgaattc gatttagata
    15421 aactttggat tcgcaacatc aatgtaacaa ctggtttggt atctacaaat acgactccac
    15481 aattgttgaa agcgcttgaa agtcataaga ttgaaccaga aaaattggta actcactatt
    15541 tcaaactcag tgaaattgaa aaagcctacg aagtcttcag taaggcagca gaccaccatg
    15601 ccattaaggt cattatcgaa aacgatatct cagaagccta agtagtaaaa atatttttgt
    15661 acataagtaa atagaaattc agtcatccat cagatggctg gattttttat caaaaaatta
    15721 agaaatgagc atatttcttt ccttgtctgg cggaattggt tataatatac ggtacaaagg
    15781 aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga ttttgaaccg tggtggttct
    15841 tagaaggttg ggaagaagat attgtagcaa gtagaaaatt tgaccagtat tatgatgctc
    15901 tcaaatacta caaaacttgc tggtttagat tggaacaaga atcgcctctt tataaaagta
    15961 gaagcgactt gatgaccatt ttttgggacc cggaagacca acgctggtgt gatgaatgtg
    16021 atgagtattt acaacaatac cattctttgg ctcttttgca ggatgagcag gttatcccag
    16081 acgaaaaact acgctcaggc tatgaaaaac aaaccagtca ggaaaggaat cgttcttgcc
    16141 gtatgaaatt aaaatagaga aaagtaactt ttttggagtt gcttttttta tttttctaac
    16201 tctttgcgaa tagtataggt gaggaggtaa gtatggttca agaaattgca caagaaatca
    16261 ttcgttcagc tcggaaaaaa gggacgcagg atatctattt tgtccctaag ttagacgcct
    16321 atgagcttca tatgagggta ggagacgagc gctgtaaaat tggtagctat gattttgaaa
    16381 agtttgcagc cgttatcagt cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa
    16441 gacgtagtca actgggttcc tgtgattatg cctatgacca gaagatagcg tctctacgtt
    16501 tatctactgt aggcgattat cgggggcatg agagtttggt tatccgtttg ttgcacgatg
    16561 aggagcagga cctgcatttt tggtttcagg atattgaaga attaggcaag cagtacaggc
    16621 aacgggggct ctatcttttt gctggtccgg ttgggagtgg taagacgacc ttgatgcatg
    16681 aattgtccaa gtcactcttt aaaggacagc aagttatgtc catcgaagat cctgtcgaaa
    16741 tcaagcagga cgacatgctt cagttgcagt tgaacgaagc aatcggccta acctatgaaa
    16801 atctaatcaa actttccttg cgtcatcgac cagatctctt gattatcgga gaaattcgtg
    16861 acagcgagac ggcgcgtgca gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa
    16921 ccattcatgc taagagtatc cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg
    16981 aagaagaatt ggcagttgtt ttgcaaggag tctgctacca gagattaatc gggggaggag
    17041 gaatcgttga ctttgcaaac agagattatc aagaacacca agcagccaag tggaatgagc
    17101 aaattgacca gcttcttaaa gatggacata tcacaagtct tcaggctgag acggaaaaaa
    17161 ttagctacag ctaagcaaaa aaatatcatc accctattta acaatctctt ttctagcggt
    17221 tttcatctgg tggagaccat ctccttttta gataggagtg ctttgttgga caagcagtgt
    17281 gtaacccaga tgcgtgtggg cttgtctcag gggaaatcat tctcagaaat gatggaaagt
    17341 ttgggatgtt caagtgctat tgtcactcag ttatccctag ctgaagttca tggcaatctc
    17401 cacctgagtt tgggaaagat agaagaatat ctggacaatc tggccaaggt caagaaaaaa
    17461 ttaattgaag tagcgaccta tcctttgatt ttgctgtttt ttcttctctt aattatgctg
    17521 gggctacgga attacctgct cccacaactg gatagtagca atattgccac ccgaattatc
    17581 ggtaatctgc cacaaatttt tctaggcatg gtagggcttg tttccgtgct tgccctttta
    17641 gcactaactt tttataaaag aagttctaag atgagtgttt tttctatctt agcacgcctt
    17701 ccctttattg gaatctttgt gcagacctac ttgacagcct attatgcacg tgaatggggg
    17761 aatatgattt cacagggaat ggagctgacg cagatttttc aaatgatgca ggaacaaggt
    17821 tcccagctct ttaaagaaat cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa
    17881 ttttctcaga cgataggaac ctatcctttc tttaggaagg aattgagtct catcatagag
    17941 tatggggaag ttaagtccaa gctgggtagt gagttggaaa tctatgctga aaaaacttgg
    18001 gaagcctttt ttacccgagt caaccgcacc atgaatttgg tgcagccact ggtttttatc
    18061 tttgtggcac tgattatcgt tttactttat gcggcaatgc tcatgcccat gtatcaaaat
    18121 atggaggtaa atttttaaaa tgaaaaaaat gatgacattc ttgaaaaaat ctaaggttaa
    18181 agcttttaca ttggtggaga tgttggtggt cttgctgatt atcagcgtgc ttttcttgct
    18241 ctttgtacct aatctgacca agcaaaaaga ggcagtcaat gacaaaggaa aagcagctgt
    18301 tgttaaggtg gtggaaagcc aggcagaact ttatagcttg gaaaagaatg aagatgctag
    18361 cctaagcaag ttacaagcag atgggcgaat cacggaagaa caggctaaag cttataaaga
    18421 ataccatgat aaaaatggag tagcaaatcg taaagtcaat gattaaggcc tttaccatgc
    18481 tggaaagtct cttggctttg agtcttgtga gtatccttgc cttgggcttg tccggctctg
    18541 ttcagtccac ttttgcggca gtagaggaac agattttctt tatggagttt gaagaactct
    18601 atcgggaaac ccaaaaacgc agtgtagcca gtcagcaaaa gactagtctg aacttagatg
    18661 ggcagacgat tagcaatggc agtcaaaagt tgccagtccc taaaggaatt caggccccat
    18721 caggccaaag tattacattt gaccgtgctg ggggcaattc gtccctggct aaggttgaat
    18781 ttcagaccag taaaggagcg attcgctatc aattatatct aggaaatgga aaaattaaac
    18841 gcattaagga aacaaaaaat tagggcagtg attttactgg aagcagtagt cgctctagct
    18901 atctttgcca gcattgcgac cctccttttg ggacaaattc aaaaaaatag gcaagaggaa
    18961 gcaaaaatct tgcaaaagga agaagtcttg agggtagcta agatggcctt gcagacaggt
    19021 caaaatcagg taaacataaa cggagttgag attcaggtgt tttctagcga aaagggattg
    19081 gaggtctacc atggttcaga acagttgttg gcaatcaaag agccataagg tcaaggcttt
    19141 taccttgtta gaatccctgc ttgccctgct cgtcatcagt ggaggcttac tcctttttca
    19201 agctatgagt cagctcctca tttcagaagt tcgctaccag caacaaagcg agcaaaagga
    19261 gtggctcttg tttgtggatc aacttgaggc agaattagac cgttcgcagt tcgaaaaagt
    19321 ggaaggcaat cgtttctata tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa
    19381 gtcagacgat tttcgtaaaa ccgatgctag tggacggggg tatcagccta tggtttatgg
    19441 cctcaaatca gctcagatta cagaggacaa tcaactggtt cattttcgtt tccagtttca
    19501 aaaaggctta gaaagggagt tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg
    19561 ttctcctcta tgcagtcacc atggcagcta tctttagtct tttgttgcaa ttttacttga
    19621 accgacaagt cgcccactat caagactatg ctttgaataa agaaaaattg gttgcttttg
    19681 ccatggctaa acgaaccaag gataaagccg agcaagaaag tggggaacag gtctttaatc
    19741 taggtcaggt aagctatcaa aacaagaaaa caagcttggt gacgactgtt cgtacggata
    19801 agagtcaata tgagtttctg tttccttcag tcaaaatcaa agaagagaaa agagataaaa
    19861 aggaagaggt agcgactgat tcaagcgaaa aagcggagaa gaaaaaatca gaagagaagc
    19921 ctgaaaagaa agagaattcc tagtcaattc aactataatg cgttgaatcc agaatagtcc
    19981 actgtagttt ctagaaaatt gctggaaatg gatgttaagc tccaattcat ttgtttatat
    20041 cttatttcag tttactatac tttgtgctaa actaaagata tgaaacatga ttttaaccac
    20101 aaagcagaaa ctttcgattc ccctaaaaat atcttcctcg caaacttggt atgtcaagca
    20161 gtcgagaaac agattgatat tttatcagac aaagaaattt tagatttcgg tggtggcacg
    20221 ggtctattag ccttgcccct agccaagcag gctaagtcag ttactcttgt agacatttct
    20281 gagaaaatgt tggagcaagc tcgtttgaaa gcggagcagc aagcaatcaa gaatatccag
    20341 tttttggagc aagatttacc gaaaaatccc ttggagaaag agtttgattg ccttgctgtt
    20401 agtcgggttc ttcatcatat gcctgatttg gatgcggctc tctcactgtt tcatcaacat
    20461 ttgaaggaag atgggaaact catcattgct gattttacca agacagaagc taatcatcat
    20521 ggatttgatt tagctgaact ggaaaacaag ctaattgagc atggtttttc atctgtgaat
    20581 agtcagattc tctatagcgc tgaagacctg tttcaaggaa attactcaga attcttttta
    20641 atagtagccc aaaaatcact cgcctagtca gggagtgatt tttctataag gatggaaaaa
    20701 agaagggaaa tttgataaga taggaatatg gattttgaaa aaattgaaca agcttatacc
    20761 tatttactag agaatgtcca agtcatccaa agtgatttgg cgaccaactt ttatgacgcc
    20821 ttggtggagc aaaacagcat ctatctggat ggtgaaactg agctaaacca ggtcaaagac
    20881 aacaatcagg cccttaagcg tttagcacta cgcaaagaag aatggctcaa gacctaccag
    20941 tttctcttga tgaaggctgg gcaaacagaa cccttgcagg ccaatcacca gtttacacca
    21001 gatgccattg ctttgctttt ggtgtttatt gtggaagagt tgtttaaaga ggaggaaatt
    21061 actatcctcg aaatgggttc tgggatggga attctaggcg ctactttctt gacctcgctt
    21121 gataaaaagg tggattactt gggaatggaa atggatgatt tgctgattga tctggcagct
    21181 agcatggcag atgtaattgg tttgcaggct ggctttgtcc aaggagatgc cgttcgtcca
    21241 caaatgctca aagaaagcga tgtggtcatc agtgacttgc ctgttggcta ttatcctgat
    21301 gatgccgttg cgtcgcgcca tcaagttgct tctagccaag aacatactta cgcccatcac
    21361 ttgctcatgg aacaagggct taagtacctc aagtcagacg gatacgctat ttttctagct
    21421 ccgagtgatt tgttgaccag tcctcaaagt gatttgttaa aagaatggct gaaagaagag
    21481 gcgagtctgg ttgctatgat tagtctgcct gaaaatctct ttgctaatgc caaacaatct
    21541 aagactattt ttatcttaca gaagaaaaat gaaatagcag tagagccttt tgtttatcca
    21601 cttgctagct tgcaagatgc aagtgtttta atgaaat
//
LOCUS       DAVHIU010000023        21591 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVHIU010000023 DAVHIU010000000
VERSION     DAVHIU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.025"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VVN06_000743"
     CDS             complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VVN06_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5562326.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2374..3243)
                     /locus_tag="VVN06_000744"
     CDS             complement(2374..3243)
                     /locus_tag="VVN06_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU5562327.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3466..3987)
                     /locus_tag="VVN06_000745"
     CDS             complement(3466..3987)
                     /locus_tag="VVN06_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5562328.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDKK"
     gene            complement(3959..5050)
                     /locus_tag="VVN06_000746"
     CDS             complement(3959..5050)
                     /locus_tag="VVN06_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU5562329.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTIITVRMLRKSGSQNSNLY"
     gene            complement(5161..5415)
                     /locus_tag="VVN06_000747"
     CDS             complement(5161..5415)
                     /locus_tag="VVN06_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562330.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5755..6288)
                     /locus_tag="VVN06_000748"
     CDS             complement(5755..6288)
                     /locus_tag="VVN06_000748"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU5562331.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(6275..6490)
                     /locus_tag="VVN06_000749"
     CDS             complement(6275..6490)
                     /locus_tag="VVN06_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5562332.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6457..6600)
                     /locus_tag="VVN06_000750"
     CDS             complement(6457..6600)
                     /locus_tag="VVN06_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU5562333.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            6834..8552
                     /locus_tag="VVN06_000751"
     CDS             6834..8552
                     /locus_tag="VVN06_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU5562334.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            8663..9010
                     /locus_tag="VVN06_000752"
     CDS             8663..9010
                     /locus_tag="VVN06_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5562335.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9289..10125)
                     /locus_tag="VVN06_000753"
     CDS             complement(9289..10125)
                     /locus_tag="VVN06_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562336.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10138..10767)
                     /locus_tag="VVN06_000754"
     CDS             complement(10138..10767)
                     /locus_tag="VVN06_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5562337.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10777..11418)
                     /locus_tag="VVN06_000755"
     CDS             complement(10777..11418)
                     /locus_tag="VVN06_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5562338.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11564..12793)
                     /locus_tag="VVN06_000756"
     CDS             complement(11564..12793)
                     /locus_tag="VVN06_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5562339.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALHIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12783..13949)
                     /locus_tag="VVN06_000757"
     CDS             complement(12783..13949)
                     /locus_tag="VVN06_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5562340.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14093..15106
                     /locus_tag="VVN06_000758"
     CDS             14093..15106
                     /locus_tag="VVN06_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU5562341.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15151..15570)
                     /locus_tag="VVN06_000759"
     CDS             complement(15151..15570)
                     /locus_tag="VVN06_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU5562342.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15581..16987)
                     /gene="atpD"
                     /locus_tag="VVN06_000760"
     CDS             complement(15581..16987)
                     /gene="atpD"
                     /locus_tag="VVN06_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5562343.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTISMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17073..17951)
                     /locus_tag="VVN06_000761"
     CDS             complement(17073..17951)
                     /locus_tag="VVN06_000761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU5562344.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(17967..19472)
                     /locus_tag="VVN06_000762"
     CDS             complement(17967..19472)
                     /locus_tag="VVN06_000762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5562345.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19487..20023)
                     /locus_tag="VVN06_000763"
     CDS             complement(19487..20023)
                     /locus_tag="VVN06_000763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU5562346.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20023..20517)
                     /gene="atpF"
                     /locus_tag="VVN06_000764"
     CDS             complement(20023..20517)
                     /gene="atpF"
                     /locus_tag="VVN06_000764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU5562347.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20531..21247)
                     /gene="atpB"
                     /locus_tag="VVN06_000765"
     CDS             complement(20531..21247)
                     /gene="atpB"
                     /locus_tag="VVN06_000765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5562348.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21282..21482)
                     /locus_tag="VVN06_000766"
     CDS             complement(21282..21482)
                     /locus_tag="VVN06_000766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU5562349.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         6464 a         4660 c         3920 g         6547 t
ORIGIN      
        1 tacaacctaa aaacagtgtt ttgagcagtc tgcagctaac tttctataac aaaatgaaat
       61 aagaacaaga caaatcaatc aagataatca aatcgatttc taacaatgtt ttagaagtag
      121 atgtgtacta ttctagtttc aatatattat agtttgctct ttgattttca ttgagtatta
      181 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
      241 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      301 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      361 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      421 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      481 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      541 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      601 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      661 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaaca
      721 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      781 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      841 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      901 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      961 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
     1021 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
     1081 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
     1141 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1201 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1261 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1321 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1381 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1441 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1501 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1561 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ttaatagaaa
     1621 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1681 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatcgatca
     1741 ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca
     1801 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
     1861 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
     1921 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagc aaaatgaaat
     1981 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
     2041 gggggactat tccaacttca acccactata aagcagactt acaagatagg gaacccatct
     2101 ccgataatat atagtagaat gaaaactgca caatttgatt ggagaattca aaccaatttc
     2161 taacaatgtt ttagaaacaa gggtctatta tttcaactgc aacctactat agcccattga
     2221 agctggaata gcccactgag acttctaaac cactgctgga gaggagtttg tttagagttt
     2281 ctttcatttc cctaaaaaat caaaaagaga gaaccagtct gatcctctct tacttataga
     2341 tggtgctcac tcaaatgtta aaggagctat ttcttagaga taaaggaatt tgaatacagc
     2401 tactgccgca attgctgctg cgataggtgc tactactggt acccaagaat accaccattt
     2461 tgaatcgcct ttatgctcac caagaactga ttttggaagg aaagcatgaa ggagacgtgg
     2521 tcccaagtca cgggctgggt tcaaggcagg tcctgtaggt cctccaagtg atgttaccaa
     2581 agccataacg aggaatccaa gtgccaagtg agccacagaa agtcctgaag cagtgtgtgg
     2641 agccacctgt gctttaatag ccaagtcaga aaaatcaact gtttgtcctg cttctattgc
     2701 cttttgtttc atgaattgaa gcacttcagc accaaagaag tttttagtca aaccaagagc
     2761 tgcaaagaac aaaacaaatg aaccaacaaa ctcattaatc aaaccattga cagttgctgc
     2821 atagcgactt tcttttgtac catggtcaat acttgaaata gttgagaaag ttcccaagat
     2881 gttatttggt ttttcagttt tcaagtagaa tggacggtat gttgccacaa ctaaggcttg
     2941 gccaaagata gcccccaaga cttgcgcgat aatgtaaggt accacttgtg cccaagggaa
     3001 aagaccgcta actgcaagcc ctagagtgaa agcagggttg atgtgattcc cagatacgtt
     3061 accaaacatc aaggctggga tcataacccc cataccataa ccaacagcga tgacgatcca
     3121 gccactttgg tgacctttcg tacctttaag ttcaacgttg gcaactgcac cattcccaag
     3181 aatgatcaaa atggcagttc ccaaaaattc agtggcatac ttcagtgccc atgtgaaatc
     3241 catttgctag attctcctta aaatttttta gatattcctt attttatcaa tttttaccct
     3301 atttagcaac tgatttctgg aaatattttt aaaaaaatag gattcatagt ttcccagttc
     3361 aacgtacttg taagcgtttt tattcttcac gaaagacctc attctcaaaa ccaggaaaaa
     3421 gcccacatct gtgatctttt ccttgttcct agattctaaa atctattatt ttttatcttc
     3481 ttttttcttg aagaatccta atcctagacc agctaacaag gtcatgatac cagcactagc
     3541 caaggtagca ttagcttctg tacctgtgtt tggcaattcc tctctcttac ctgtctcata
     3601 agtcggaact tctgggtctg gattcactgg agtttcagtt tttggagtac ctggttctgg
     3661 agttggttta tctggtgttg ataaacggtc ataccttacc gttatttctt tatcactaga
     3721 gtctgacgta acttcttgtg attcaactgt tggaatatct ggatctttgt acttgtcaat
     3781 cttaccagat ataacctcgt cccagtttcc tgttgtccat tcaccgtagg ttacaactcc
     3841 cgtgaccttg ttctcagttt ttgtacggct taaggttaca ggttgaacaa catcttcttt
     3901 tacattttgg ttcgtaactt tatcaacgta atgaatgata cgcgttatag tcttcgtctc
     3961 agtagaggtt gctgttttgg gaaccacttt tcctcaacat tctcacggta ataatagtca
     4021 actgttgcac cgtcttctgg tacgcatttg caggagttgc taccaaggtg tatgttgttt
     4081 tccttgtgat aactcggtct tctttgtcct cagttgttgt tttcccttca atagtttttg
     4141 attctgtggt atactcagaa cctatcgcta aatcagcttt tataacagac tctgccaact
     4201 tctcttggct accttcttta tagtaattgg ctgttactgt agcagtggtt ggcgcttcgc
     4261 tttctctata aactaaggtc actgttctac cttcgcttac aatattccca gttaaacttg
     4321 cagaatttgt atctgcttct ttaaaagtat aatattttcc gtcagtagta gtcatgctac
     4381 tgagtttttt atctgtgaca ttgtagctgg taccaatcag ttgtttttta ttggtaatgt
     4441 aggttccgtc actttctttt tctccaattc cagtatcatt tttcaatgat agcaaacgcc
     4501 cttgttcatc aacatagcga actttcacat tttctgagat tagttctgcc aattctgagg
     4561 tttttttctt tttcttgatt tcttcggtta ttttcccttt ctcttcttcg ggaatattta
     4621 gttttggaat gattttttca acaacggttc gtgatggttc cacagtatct tggatgactg
     4681 aaaagtcagc tagaattggg agattatact gaacacggtg actttgagtg tttactccta
     4741 ctctttcatt attctctgaa aatacttgta cggtataaga aacaacatct tttcctaata
     4801 gaacatcccc agtagagaaa tagccgcctt ttcctagttt gctatctcca gagcccactt
     4861 ctttcctaat cttatcagat agttttttac cagtcagtac attcgttcgc acaatccctt
     4921 tgtctacccc tacaaagtgg gagaactttt tgaactcttc agaaccagat ctagcccaac
     4981 cattattaag ggcatttgct tttgtatttg tattctctct caaaagtttg gcgattagaa
     5041 ttatattcat cggcacttag agttgctgct atatctgact cttgaatacc aacttcctta
     5101 ctaccatttc tagcggcagt atatgtgtat ttatttgttt ctacttctag ctgattatcc
     5161 ttaacatgat aggtaatttt ctcctggtat aagagtcttc agtagtttat atgctctgtc
     5221 atcatcttta gctttagaag cagctgagtt tagccaagtt ggggtattag gagtatcttt
     5281 atcagcctgt ttcaagacat tattcaagtc atttgctagc ctattggttg tcctcactgt
     5341 gaaagttttc ttccagtctg aaccactttt attagactta gatatgctca tgaaatcctg
     5401 gtcaacattc aacacaatat cgccttgttt tttagcatct tccgttgctt ctgcttcaaa
     5461 ttgatcatgg aatttttgtt gctctggtgt atagtttttt tatttacacg atttaatgta
     5521 ctatttttta ttttagtatt aagaactcca ttaatacgat ttttaaactc tgcttcaaaa
     5581 aatcccttaa tttgttggtg cgcaagttca acattcaaac ctttcttata caagtcttca
     5641 ttctgaacga tttcggctac acggtgaatg gtttgatcgt ataccgtctt ctcttaacca
     5701 gcagtatcct tagttttaat gactactcga taagttcctt tagaaccgtt atagtcatct
     5761 agttttaaaa aagcgttcca aacattcaaa aatagttgga acttccctta ataaatccgt
     5821 cactttcttt ttcaagatag cccaagcttg ctcaatagga ttcaaatccg gtgaataagg
     5881 tggtagaggt aagaaaaagt gtttatcttg gatgcagagt tcatccaaaa tgttcttggg
     5941 atgaaaacta gcattgccca tgacaataac atgaggtgtc ttcaaagcag gtaggagttg
     6001 cgttttgaac cactccacaa agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc
     6061 tataaactct ccgtctactt gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct
     6121 aatcttttca tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg
     6181 gtcgattcct gtttcgtcaa tatatacgat tggtaaatct tttaggttat ccaaaatatc
     6241 aagaaactca gcgactttct ctggctcttg ttccttaaag ctcgtcatct ttttttaaag
     6301 tgaccttaat ctgctttaaa gctgcccata ctgaaggaac agcacaatca aaatgtgccg
     6361 caatttcccg taaaaaagca tctggatgag cctctacaaa ggctttcaat tcctctaaag
     6421 gaatctttcg gtttttgacg actcgctttt tcctctctat gtgtccttgt tcacatcttt
     6481 cttttcccac gtgaagagag ttctgacgcc aacaccaaaa aacttggctg cctcgacatg
     6541 gctgtgcccc tctttgatgt aatctaatgc tcgctgttta aaatcagtac tatatgccat
     6601 agctttatta taatatgctt gtttaaaact aagcaactat atcaatgttt tgttattaga
     6661 agacttactt tttttactga aatcgaattt ttcctaacct caacatcata taaccccaaa
     6721 attcaatctg acctggtttg aagagatgtt cgaagcagat aaggttttac taaaactaaa
     6781 aaaaatgata taatcaaact gattaaatgt tttaaaaata aaggagaatt tgaatgtctt
     6841 accaagaaaa ttaccagaaa tgggttgatt ttgtggagct tcctgactac cttcgtcaag
     6901 atttggaaaa tatggacgaa aaaactaagg aagatgcctt ctatacaaat cttgaatttg
     6961 gtactgcagg tatgcgtggc ttggttggtg ctggtacaaa ccgtatcaac atctacgttg
     7021 ttcgccaagc tactgaagga ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag
     7081 aacgcggtgt agcaattgcc tacgatagcc gtcacttctc acctgagttt gcctttgaat
     7141 ctgcggcagt tcttgctaaa cacggcatca aatcttacgt atttgaaagc cttcgtccga
     7201 ctccagaact atcatttgca gttcgtcatc tcaactgttt cgcaggtatc atggtcacag
     7261 ccagccacaa ccctgctcca tttaacggtt acaaggttta cggtgaagac ggtggacaaa
     7321 tgcctccaca cgatgcggac gctttgacta cttatatccg tgcaatcgaa aacccatttg
     7381 cagttgaagt tgctgatgtg gaaactgaaa aagcttctgg cttgattgaa gttatcggcg
     7441 aagctgttga cgtagaatac cttaaagagg ttaaggacgt aaacatcaac ccagccttga
     7501 ttgaagaatt tggtaaagac atgaagattg tctacacacc acttcatggt actggtgaaa
     7561 tgttggctcg tcgtgctctt gcccaagcag gatttgactc tgttcaagtt gttgaagcgc
     7621 aagcaactgc tgacccagac ttctcaactg taacatctcc aaacccagaa agccaagcag
     7681 cctttgccct tgctgaagaa cttggtcgtc aagttggtgc agatgttctt gtcgcaactg
     7741 acccagacgc tgaccgtgtt ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc
     7801 tttcaggtaa ccaaatcggt gctatcatgg ctaaatacat cttggaagct cacaaaaacg
     7861 ctggaactct tcctgaaaat gccgctctct gcaaatctat cgtttcaact gacttggtaa
     7921 cgaagattgc tgaaagctac ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta
     7981 tcgctgaaaa aatccaagaa ttcgaagaaa aacacaacca cacttacatg atgggatttg
     8041 aagaaagctt cggttacttg attaaaccat ttgtacgtga taaagatgcc atccaagccg
     8101 ttcttgtcgt tgctgaactt gctgcctact accgttctcg tggtttgaca cttgctgacg
     8161 gtatcgaaga aatctataaa gaatatggct actacgcaga aaaaacaatc tctgttactc
     8221 tttcaggtgt cgatggtgct gaacaaatca aagcgattat ggctaaattc cgcaacaatg
     8281 ctccaacaga atggaacgca acagctatca ctgtcgtaga agacttcaag gcacaaactg
     8341 ctactgttgc tgacggtact gttacaaact tgacaactcc tccaagtgat gtgttgaaat
     8401 acacacttgc tgacggttca tggattgccg ttcgcccttc aggtacagaa ccaaaaatca
     8461 agttctacat tgcagttgta ggggaaacca acgaagaatc acaagctaag attactaaca
     8521 tcgaagcaga aatcaatgca tttgtaaaat aaagacctat aaaagatgag accaaccaac
     8581 ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac tttatggcat actagactta
     8641 tcaatgaaag cgaggtaatc tcatggaaca atttttagat aacatcaaag accttgaagt
     8701 cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa ccttctttat
     8761 cggtcgcaaa acttgccctt actgccgtaa atttgcaggt acattgtcag gtgtcgtagc
     8821 tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa ccaagccaac tcaatgattt
     8881 gcaagcattc cgctcacgtt atggaatccc aactgtacca ggttttattc atattacaga
     8941 tggacaaatc aacgtccgtt gcgactcttc aatgtcagca caagaaatca aagatttcgc
     9001 aggattgtaa aaaatcagct aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc
     9061 aaaaaccttg atattatgcg ttttattgtt cctaaaacag ttacatcagc atttcattac
     9121 agaacatttt aattttggaa gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg
     9181 gctattccat attcaattat ccagattcaa tcgctattat cgttttccta gattacaaaa
     9241 aaaactacgt tttgctaatc aaaacgtagt ctcagccact attttatctt actgtccttc
     9301 ttttacttct ttggttgcta catcttctcc aaaccatttt tggctgattt cttggaactt
     9361 gccgtccttg taaagactag aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc
     9421 cttacgggat ccaaccgcaa aagcttctgt ttctagtcca actgtaaaga cattataatc
     9481 gtttaaaaca ccttctgctt ctaaataata gtttgcatag acacggtcaa tcaatagacc
     9541 atcaattcga tcgtttttca aatcaatcaa ggcttcatta aaggtttggt attgattcgc
     9601 ttccttatta gcgacaatat tcttcaaaat ttctggattt gcttcaaagt ccgcataacc
     9661 agatgaacca gcttgagctc ctaatgtctt tccagtcata tcctttgcag tcgtgatacc
     9721 agatgatttc ttcgtaacca atacctgctc attcttcata tatgagttac tgaaagccac
     9781 cttttcacgg cgttcgtctg tagcggaata gccattccaa atcagatcaa tcgttccttt
     9841 tgtcaattca gcttctttca aatcccaatc aatcggttgc caatttaccg tgattccgta
     9901 tttttcaaaa acagctgtag ctaaatcaat atcaaatcct gcataagaac catctttctg
     9961 agcaaatccc attggaacaa aagtactatc aaatccaata gtaatagact tgttagactg
    10021 gtactttgac caattatctc cactagtttc gctagaattt ttcccacaag ctactaagaa
    10081 caaagcagtc atcagactga ctaatacaag catccatttt ttcatccatt tttcctccta
    10141 tttaggttct actttcaata atacatctgc gatattttca gcaaactgca aatcatgggt
    10201 aaccacaatc tgggtcatcc caagttccct attttgcaag attagcttct ccacttccaa
    10261 acgtaattct ggatccaggg cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat
    10321 catcatagca cgcgccaaag ccacccgctg cttttgccca ccagatagtg agaaaggata
    10381 ggcctctgcg tgtcctccta gtcctaactg ttccaagagc ccactcgcct tcttctcagc
    10441 ctcttcctgc ttcattccca tggtcttcac aggcgataaa gtcaaatttt ccagaactga
    10501 tagatgagga aatagttgaa aatcttggaa gacaaatccc agtaggttgc gcttctgcaa
    10561 ttcatccagc tctaaaggtt gtccattata aaagatttgc cctgaatcaa tggtttcaag
    10621 acctgcaagc atacgtaaaa gagttgtctt acctccacca gaaggtccaa cgatagccag
    10681 gatttgcttt tcaggaatac ttagactgaa atcagacagg atttgtttgt ctccaaagac
    10741 tttattgata tttcgtaatt ctaacatagc tgccccctat ctataataac tatacttctt
    10801 ctcaactttt ttggaaataa ttgtcacaat cccaattaaa atcaagtaaa tggctcctgc
    10861 caagaacata ggaaccagac tagcatcgcg gttagcagct gttcgactag ccaagataag
    10921 gtctgaaatt ccgagagcat agaccaaaga agtatccttg accaaactca taacttcatt
    10981 aaagacacta ggaagaacga tcttggtcac ttggggcaag ataatatagc gcactctgtc
    11041 aaaagggcta aacttcaaga ccttggcagc ctcatactgt cctcttggaa tagtgtcaat
    11101 tcccccacgg aaaatttctg caaagtaagc tgcatagttg agaacaaagg caataatagc
    11161 tgcaggaagg cggtctaaac gaatcccaat acttgggagc acataataga taaaaatcag
    11221 ttgcaagagt aagggggttc ctcgcataac ccagatgtaa atgttaatca gataatggag
    11281 gggcttccaa tggacttgca aggcaaaggc aatcaaaacg cccaacggaa tcgaaaatag
    11341 caagaccagt gcaaagacct gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat
    11401 ctcaaataaa taagacatac tgcacctcct aaaagtaatt gttcctatta tagcatgaat
    11461 aaacaagatt accaagtatt tttgtaaaaa gattctacca tatacaaata aaaaccgtag
    11521 ctatcaaaag agataaacta cagttctata tgtttaaaca ttcttacaat ctctcaaaca
    11581 gttcttgcat ttgcacatca tctggaacca gttttaagta agtgtgagca tggacttttg
    11641 cttcttcaaa atgtcccaat tcacgcaaga gatagatata gtgttccaga aattctggat
    11701 tgtccttcaa atctcctgtc aactcttgat aatgctcata agcagtatcc aaatcgtcca
    11761 tttcttgata agaacgagca atcatccact tggtcaaaag attttctggc tcctcactct
    11821 gcaagtctag aatatcctca taacgctcct gctccagata aatagtggct aaacgaagca
    11881 agatttcttc tgtatcctca gcgtcttctt ttgcagtaag gagataattt tctgcaccac
    11941 tagcatcatg caattcataa gaaaattgtg aagcagctag caagaggcga gtttcaaagg
    12001 gatttttctc taatccttgc ttagcgatat gcagggcttc ttgaacttga tgttccttat
    12061 gtaaagcctg actgtaccca tactcatagc cttcaaagtc aggagaaatg gtatcaagct
    12121 gcttaaagta gagggtggct ttttgatatt cttcttgatc aaaataaaga ctggccaact
    12181 caaaagctgt taagtcatcg tattctaact ccagggcttt ttctaaaaac tcagtagccg
    12241 tttcaaattt ccctaactga gcataggcaa agccaattcg ttgataggtg gaaatgcccg
    12301 tttgctcata aatcgagcga ttatctaact gggcataggc ttgaatagcc gcttggtaat
    12361 tttccaactc actatccaac tctgccaaac ccaatatcaa gagagaatcc tctgagtagg
    12421 tcaaggcctc caataatttc tcacgtgcca catctgtcaa accttccagc tggtaaaggt
    12481 ctgccttcaa aaccaaagac gagacatacc agtcactgtc agcttggatt tcctcaagat
    12541 aggtaaaggc ttcttctatt tgaccatcct cgctagcaat tgcagctaga ttaagatgaa
    12601 cctctggaaa atcctctaca attttcaggt aaatttcctt ggcctgagga tagaaaccaa
    12661 tcccttcaag ataagttgcc aactcataca gaagatcact tgaatcattt tctaaagctt
    12721 tggcgaaata atgctcagcc ttagttaaat cttgctcctc caaagcctgt aacatctgtt
    12781 gactattgtt cactcttgac ttcctcaccc tctaattcat atagaccact gactacctta
    12841 taccattcga aaatggctga aatgacaacc ttagcagagg cataaactgg aataccaagt
    12901 aaaactcccc agataccaaa catagatcct gaagttaaca aaacaaagag aacattaata
    12961 gggtggatgt ttaattgact tcccaaaatc aatggagaga caaaacggcc ttcaatagtt
    13021 tgttctacaa taaagacaat cactactttc aaaagcatga ctggaccagc aatcaaaccc
    13081 aatactaggg caggaagcat ggctagaaag ctaccaagat aagggaccag atttaaaata
    13141 ccagcagtaa cccccagcgt aaccgcatag cgtagaccaa taatcttgaa gaagatgata
    13201 aacattactg ctacaataat agccactgtc acttgccctc gaacatagtt ggacaactgt
    13261 tgattcacat ctgatagaac ttgtccaaca ggttccttca attttcttgg aatgaattgg
    13321 gtcaaatagt tacgcaagcc tttcccatca cgcaagagat aaaagagcat gaaaggaacg
    13381 ataatcaagg caacaatcac ttgagaagcc ccgctaataa aggcactcac ccagttgact
    13441 gcctgagatg aaaccttact tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact
    13501 tgctctaatt gaggtctgaa atcatctggc agatgctggg ctaccaatcc attaacaatc
    13561 ctatctatat cttctaagta aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc
    13621 agatttggaa tggcgactgc caagccccaa atgataaaga gagcgatgat aacaaagaca
    13681 atagtgatag ctataacacg attaacctta tgcttctcca tccaatcaac aataggattc
    13741 aacaaataat ataacaaacc agacaaaatg actggcaaca tcacaactgc taaaaagtct
    13801 aaaacaggtg aaaatagaaa actaatctta cttaaaataa aaagattcaa tcccaataat
    13861 aaggttacta aaaataccgt aattgccttg ttatctaaaa accacttgaa aaaccaagat
    13921 aggctaaaat gtttctcttt ttgctccata aatacttcct ttctattgtt ctctcattat
    13981 accatttttg aaacaaaata actctttttt aaagtgctta catctaattg gatgtcttta
    14041 atttgcttgt ttttgtgata taatagtctt atctttacat agaggtatag taatgaaaga
    14101 aactgtttat tttggaactt atacacgtcg tacttctcaa gggatttaca aggcagactt
    14161 tgatacagaa actggtcagc tttcaaatct agaacttttt gcagctgagc caagtccaac
    14221 ctaccttgcc tttgaccagc accaacattt atacactgtt ggtagccaag acgataaggg
    14281 gggaattgca gcctatcaaa ctgacgggac tgtgttaaat catgttgttg aagaaggagc
    14341 tccccactgt tatgttgctg tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca
    14401 caagggacaa gtccttgttt ataaacgcca ggaagatggt agtcttctac ttagtgatat
    14461 ggatcaacac agtggccaag gtccacatga aaatcaagct tccccccatg ttcactatac
    14521 agatttaaca cctgaccact atctagtgac ctgcgacttg ggaactgacc aagtcatcac
    14581 ctatgatctc gatcaagaag gaaaattatc taagctctat acctatcaca gcaagccagg
    14641 agcaggctca cgccatatca ttttccataa ccactataaa atcgcttatc tcatttgtga
    14701 actcaatagt actatcgaag ttttaatcta cgatggcgtt ggcgaatttg tacgtatgca
    14761 ggttatttca actttaccag aagcttacga aggctttaat ggaaccgctg ctattcatct
    14821 ctctaaagac ggtaaatacc tctacgcttc taaccgtggc catgattcta tcgcagtata
    14881 taccatcctt gcggacggta gcttagagtt attagaaatc gttccaacgc atggtcagac
    14941 tccacgtgat tttgatttga cacccgacca aaaatttctc attgttgtcc atcaagactc
    15001 tgacaatgca actgtcttta aacgtaattg tgacaatggt cgtctagcag aactctccaa
    15061 cgacttccat gttcccgaag cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa
    15121 tgaacttggt attttcaagt tcattttttc ttatagtcta tttccgacat taatacggtt
    15181 aatggcacgt tgcaaagcaa tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg
    15241 tgcttcttca attgcacgtt ctgcacgaag tttggcacgt tctgcacgac tgatatcgat
    15301 atcacgagca cgttctgcag agtcagcgac gattgtaatc atatcattgg caatttcaat
    15361 aacgccaccg tttactgcaa tccagttcac gtgatcttta tcatcgatac gttttacctt
    15421 tacttcatca actgctaaaa ccgcaatcat attttcatgt cgtggcaaga tccccatctc
    15481 accatccaga gttcgaaccg atacatagct ggcatggtga tcatagacga gaccatctgg
    15541 tgtcacgatc tggacagtta actgagccat aggtcacctc ttaaaatccc attttttcag
    15601 cttttgcaat cacatcttcg atagaaccta caccacggaa ggcatcttct ggcaagtggt
    15661 cgtatttacc atcaaggatt tccttaaagc cacgtacagt ttcagcaact ggaacataag
    15721 aacctggctg accagtaaat tgttccgcaa cgttgaagtt ttgtgacaag aagaactgga
    15781 tacgacgggc gcgagcaacc aaggtctttt cttcatcaga aagctcatcc ataccaagga
    15841 tagcaatgat atcttgcaat tcatggtaac gttgaaggac acgttttact tcagcagcaa
    15901 ctgcatagtg ctcttctcca acgatttcag gtgccaaggc acgtgagctt gaagcaagtg
    15961 ggtcaacggc tgggtagata cccaattgta ccaatttacg ttccaagttt gttgttgaat
    16021 ccaagtgagc gaaggctgtt gctggcgctg ggtcagtata gtcatccgct ggcacataga
    16081 tagcctggat agaggttaca gaacccttct tggttgatgt gatacgttct tgcaattgac
    16141 ccatttccgt agcaagtgtt ggttggtaac caacggctga tggcatacga cccaaaaggg
    16201 cagatacttc tgaaccagcc tgagtgaaac ggaagatatt atcgataaag agaagcacgt
    16261 cttggccttc cacatcacgg aagtattcag cgattgtcaa accagtaagg gcaacacgca
    16321 tacgtgctcc tggtggctca ttcatctgac caaagaccat ggctgttttc tcgataacgc
    16381 ctgattcttt catttcccag taaaggtcat tcccctcacg agtacgttcc ccaacaccag
    16441 taaatactga aataccaccg tgctcttggg caatgttgtg aatcaattct tggattaaga
    16501 cggttttacc aactccggca ccaccgaaaa gtccaacttt accaccttta aggtaagggg
    16561 caagaaggtc aataaccttg atccctgttt caaggatttc agaagaggta gacaactcat
    16621 caaaagttgg agctttttta tgaattggct gacgctctgc gtcttctgta aaaggagctt
    16681 ccaaatcaat ggtatctccc aaaacgttga agacacgtcc caaagtttct ttacctactg
    16741 gtacagagat tggacgacct gtgtccaata cttccattcc acgagtcaac ccatctgttg
    16801 attccatgga gatagtacga accataccat ctcctaactc caaggctact tcaaggacga
    16861 tttttgtttt tctttcgtca tttttgtaga cgacaagtgc attgttaatc tcaggaagtt
    16921 tttcccctgc tgcaaacaaa acgtctacaa cgggaccgat aacctgagca attttacctg
    16981 aactcatctc cttctcctat tctatataag atactgtcgg ttcctagctc aactaggtcc
    17041 taagttcatt ttcatacgag ctggactaga gcctattcta aggcactagc acctgctacg
    17101 atttctgtaa tttcttgtgt aatcgccgcc tgtctggcac ggttatactg aattgtcaaa
    17161 tcattgatga ctttcttagc attatctgtc gctgtttgca tggctgtcat acccgcagca
    17221 ttctcagctg tcttggcatc gataatggca ccgtaaatca tactttctgc aaactgaggc
    17281 aacaactgct ccagaatttc ttctcggctg gtttccaatt caaaagtcaa gctgtactct
    17341 tcatccgcct catttggatc caagtcaaca atcggaagca tttgttccac acgcatttga
    17401 ctggttagcg tattgacatg gtggttgtag caaacataaa gctcatcaaa gagttcattt
    17461 tggtacattt caacagtttt tgaaataatc ttacgaactt gatcaaagct aggttggtct
    17521 gccaagccac gtaattcata aagtggttga ataccgcgag ccttaaagaa atcagctccc
    17581 atcccaccga tacagatcat ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac
    17641 tccataacag ctttcaaaat agaggaatta taacctccaa ccaaaccgcg gtctgaagtg
    17701 ataacgatat agcctgtctt cttcacagaa cggctaatca acatcggatt agttgaagca
    17761 ccagctccat taccatgaag gatatctgtc aaaagtttac gcactttctg agcgtaaact
    17821 tggaagttgc gagcagcttc ttcagagcga cctaacttag cagccgatac catttgcata
    17881 gcattagtga tttgactcgt attttttgtt gaggcgattt ttgttttaat atcatttaga
    17941 gacactgcca tctgacacct ctattcttat tggaagctag attgattgag aaactctgta
    18001 atcgcagcat ccaagactgc ttcttctggc aagtcttttg tatcacgaat ggtttccaaa
    18061 atctctggat gttgagcatc aaagaaggca tggaactctt cctcgaaacg aacaatatca
    18121 tctactggaa cagtatccaa gaaaccatgt gtcaaagcat aaagaatggt tacttgtttc
    18181 tcaacaggta atggtttgtg aacaggttgt ttcaagacct caacggtacg acgtccacgg
    18241 ttcaacttag cctgtgttgc tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac
    18301 tcacggtatg aagcaaggtc gatacgaagt gtaccagcaa ccttcttcat ggctttgatt
    18361 tgtgcagaac cacctacacg agatacagat gaacccgcat cgatggctgg acgaatacct
    18421 gcattgaaga ggccatcgcc aaggaagatt tgtccatcag tgatagaaat cacgttggtt
    18481 gcgatatagg ctgagatatc tcctgcttgt gtctcgataa atggtagggc tgtaattgat
    18541 ccaccaccaa gttcatcaga aactttagct gagcgctcaa gcaaacggct gtggagatag
    18601 aaaacatccc ctgggaaggc ttcacgacct ggaggacgac gaagcaagag cgacagttca
    18661 cgataagcta ccgcttgttt tgatagatca tcgtatacaa tcaaaacatg cttaccttga
    18721 tacataaatt cttccgccat agcaacccca gcataaggag ctaggaagag caatggagat
    18781 ggttgtgaag cagaggctgt cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt
    18841 gtttctactt gcgtacgaac tgttgattct ttttgtccaa tcgcgacgta gatacagatc
    18901 atatcttgat ctttttggtt caagattgta tcaatcgcaa tggttgtttt ccctgtctga
    18961 cggtcaccga taatcaactc acgttgacca cgaccaatcg gtacaagggc gtcaatagct
    19021 ttcaaaccag tttgcaatgg ttctgaaaca gacttacgtt gcataacacc aggagctggt
    19081 gcttctactg gacgagtttt atcagtgtgg atttctccaa gaccgtcaac tggacgacca
    19141 agcggatcca caacacgacc aatcagactt tcacctacag ggacttccat gattttccct
    19201 gtacggcgga ttgtatcgcc ttcacggata tctgtaaagt cacctaggat gataatacca
    19261 acgtctgttg actccaagtt ttgagccata ccataagagc cgttttcaaa attcaacaac
    19321 tctccactca tgacattttc aaggccatga gcacgcgcga taccgtcccc gatataggtt
    19381 acaacacctg tttcagtcac atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt
    19441 aaagcgctga tttcttgtgc gttaattgcc aaaagaacac cactttctat ttcaaatttt
    19501 ctttaacaac tttaagttgt tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa
    19561 tgacaaaacc accaatgaga ctttcatcga tttgttcttt tacactcctt actttcagag
    19621 acattttttt ctcaatcaaa gggagcaaac gagtcttctg ttcatcagtt agaggatgag
    19681 cagacgtaat cgtcacttca aatcgatttg tttctttttc aagtcggttc aagcaatcta
    19741 caagcacatc ataaaaaaga tttgctctgt gattgtaggc cagaacctgg ataaagtttt
    19801 gtaataaagg tgacacagaa tcttggaaaa aagcaattgt tttttcctta tccgactcgt
    19861 ctactgccac ttgttttaaa aaagaaggca gacctgtttt ttcaacaact tgcttgattt
    19921 gagtcaagtc tgaaaagata cggtcttctt ctcctttttc aagtaccaat tggacaaaag
    19981 gcatgctgta tttttcaatt acctttactg ttttcttgtc cattaagctt ctcctagctg
    20041 atcgatatac tgatcaatga gtgctttatg ggcatgactg tcaaggtttt gtgagatgat
    20101 tttaccagct aagctgatgg tcaaatctgc gacctcaccc ttaacactct gtaaagcttc
    20161 tactttattt tgagcaattt cttgattggc tttttctttt aagtgtcctg cttctagttt
    20221 agcatctgct aagatattag ccttactttg ctcagctgtt tcctttgcat tttcaatgat
    20281 tgtcttagct tctttacggc taccagccaa ttcatcttcg cgtttttgag ccaatacttc
    20341 tgctttttga cgggcttctt cagctctgtc aatatctgaa gcaatttttt cagctctttc
    20401 ttcgaaaatg cctgtaatat tagaccatgc aaatttttta atcaagacta gcaaaagaat
    20461 aaaagagcca gtgattaaaa taaaattacc aattaattca cctactgtta cgtgcatcag
    20521 ttactccttt ctattcctct tcaatattaa tcttattccc taaatacaca gatgtcaaaa
    20581 gagtaaaaac ataagcttgg atgcaggaaa taaagacaga aaatgcagtc caagccaaat
    20641 tagctccaaa ggctactgga taccaataaa tagcttggtg ggaaagaaga agtaacaaac
    20701 tcgtcatgac ctctcctgca aagatattcc caaaaatccg caaagccaaa gataagaagt
    20761 ttgtaaattc ttccaagata ttcatcggta tgacaaaaac aggactcata aaagatttta
    20821 tacttttttt aaatccacga cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa
    20881 aagataaggt taagtcatac tgtaaattag cggttggcga actccaccag ttagtcccat
    20941 cgatcgtttg aagctttgtc attaagccaa ggttattggc aatcaccatg aaaaggaata
    21001 aacaaaggaa aaagagtgag taatctttca tgtagcgcga accaatgtta ggttctgtaa
    21061 atccaataac aaagtcatag acatactcaa gtacattttg ctttcctttg ggtttcaagg
    21121 tcatattgcg gcttgcccaa taaataaaga caaaaataac tcccacaatc aacaaagtca
    21181 tggctaacat agtcagattg aagataacag gaccaataga gatgattgga ttaatacttt
    21241 cttccatcta aaatcctccc ttttcttctc gttccatgta tttatttgat aataaatgag
    21301 aagacaagag ttacaaagaa agttccttca ataaaggcaa cacctaaaaa catcaaacta
    21361 cgaaactcag aaagcatatc tggttggcgt gctactgatt taaacagtcc attcatcaat
    21421 aaaccttcac cgacagatac gcccatacag gcaatacata agcctaaaaa tgttaaattc
    21481 ataacaaatt ctcctttaaa ataaaattta cttacttagt gtagtcctaa aatttaaatt
    21541 tgtcaacttt taaccgctat tggaagcgtt taataaaaat tatttccatt t
//
LOCUS       DAVHIU010000024        21394 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVHIU010000024 DAVHIU010000000
VERSION     DAVHIU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.026"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            227..653
                     /locus_tag="VVN06_000767"
                     /pseudo
     CDS             227..653
                     /locus_tag="VVN06_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            724..882
                     /locus_tag="VVN06_000768"
     CDS             724..882
                     /locus_tag="VVN06_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU5562350.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(1250..2977)
                     /gene="ezrA"
                     /locus_tag="VVN06_000769"
     CDS             complement(1250..2977)
                     /gene="ezrA"
                     /locus_tag="VVN06_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU5562351.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(3059..5005)
                     /gene="gyrB"
                     /locus_tag="VVN06_000770"
     CDS             complement(3059..5005)
                     /gene="gyrB"
                     /locus_tag="VVN06_000770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5562352.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTSLTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(5020..5583)
                     /locus_tag="VVN06_000771"
     CDS             complement(5020..5583)
                     /locus_tag="VVN06_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5562353.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5668..6222)
                     /locus_tag="VVN06_000772"
     CDS             complement(5668..6222)
                     /locus_tag="VVN06_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU5562354.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(6233..7456)
                     /locus_tag="VVN06_000773"
     CDS             complement(6233..7456)
                     /locus_tag="VVN06_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5562355.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7493..9943)
                     /locus_tag="VVN06_000774"
     CDS             complement(7493..9943)
                     /locus_tag="VVN06_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU5562356.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQKNDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(10143..11072)
                     /locus_tag="VVN06_000775"
     CDS             complement(10143..11072)
                     /locus_tag="VVN06_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU5562357.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            11212..11499
                     /locus_tag="VVN06_000776"
     CDS             11212..11499
                     /locus_tag="VVN06_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU5562358.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFSAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(11533..12867)
                     /gene="ciaH"
                     /locus_tag="VVN06_000777"
     CDS             complement(11533..12867)
                     /gene="ciaH"
                     /locus_tag="VVN06_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU5562359.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12857..13531)
                     /gene="ciaR"
                     /locus_tag="VVN06_000778"
     CDS             complement(12857..13531)
                     /gene="ciaR"
                     /locus_tag="VVN06_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU5562360.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13640..16186)
                     /locus_tag="VVN06_000779"
     CDS             complement(13640..16186)
                     /locus_tag="VVN06_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5562361.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVCSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(16309..16749)
                     /locus_tag="VVN06_000780"
     CDS             complement(16309..16749)
                     /locus_tag="VVN06_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU5562362.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16724..17593)
                     /locus_tag="VVN06_000781"
     CDS             complement(16724..17593)
                     /locus_tag="VVN06_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU5562363.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(17571..18071)
                     /locus_tag="VVN06_000782"
     CDS             complement(17571..18071)
                     /locus_tag="VVN06_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5562364.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(18082..18780)
                     /locus_tag="VVN06_000783"
     CDS             complement(18082..18780)
                     /locus_tag="VVN06_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU5562365.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18773..19039)
                     /locus_tag="VVN06_000784"
     CDS             complement(18773..19039)
                     /locus_tag="VVN06_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU5562366.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19105..20031)
                     /locus_tag="VVN06_000785"
     CDS             complement(19105..20031)
                     /locus_tag="VVN06_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU5562367.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20106..20921)
                     /locus_tag="VVN06_000786"
     CDS             complement(20106..20921)
                     /locus_tag="VVN06_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562368.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20925..21245)
                     /locus_tag="VVN06_000787"
     CDS             complement(20925..21245)
                     /locus_tag="VVN06_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5562369.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6455 a         4649 c         3847 g         6443 t
ORIGIN      
        1 tgaacttaca atcgaaaaat ggttaagtaa aagtcaacag ttactatagt ttgcaaatta
       61 tttacagaaa gaacaaaaat tcctaggaga ttattctggc tatgtgcatt gtgatatgtt
      121 gcggcagtaa cttaggactt tagtcctcta gttctgccta tgcgatagca gtccaaggtt
      181 taggagcaag gcgacgctaa gcttggtaaa ctacgaaccg ctagaagctt atcgtcaact
      241 ggaagaagct gcacttgttg gatgttgggc gcatgtgaga aggaagtttt ttgaagcgcc
      301 ccccaagcaa gcggataaat catcgttagg agctaaaggt ttagcttatt gtgatcagtt
      361 atttgccttg gaaagagact gggaggacat tgtcagctga tgaacgacta cagaaacgtc
      421 aagaagagct ccaaccccta atggaagact tctttgcttg gtgccgtcgt cagtcagttt
      481 tagcaggttc aaaactagga agggcaattg aatacagcct caagtataaa gaaaccttta
      541 agactatttt gaaagacgga catctggtcc tttccaataa tctagctgaa tgcgccatta
      601 aatcattggt tatgggacgg agtaaaagag tccagtggac tcttttagcc taagctcagt
      661 ttaaaaaagc gagggtggtt gttttctcaa agttttgaag gagctaaagc aagagctatt
      721 attatgagct tattggaaac agctaaacgt catcaactaa atagtgagaa atatctatcc
      781 tatcttctag aatgtcttcc aaacgaggaa actctcgtaa acaaagaggt tttagaggcc
      841 tatttaccgt ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc cagattagga
      901 actatccgtg agttctctag tctggagatt tttcaataga cttcgttatt ggacagttac
      961 caagttactc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
     1021 taaatgaaaa tcaaaaagca aactaagagc ctcaggctgt acttgagtac ggtaaggcga
     1081 cgctgacgtg atttgaagag attttcgaag agtataaaac actgttttga ggtggtggat
     1141 aaatcggctg ggcaaaaact caatctcagg agaaaatgaa gtaaatctcc ccacaactat
     1201 agaaaaagat ttgattctgc tcctcacaca ataaaatctt tttcttttat taaaaacgaa
     1261 tcgtttcacg tgttttctca tatgaggtaa caaagcgatt ggttacacca ggctctgcca
     1321 cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata atcaaattct ttttcaaaaa
     1381 tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc aaatgagcga tagcggttag
     1441 aatattgcaa gagttgctct gtcaaagttg catattgtac aatattataa gtttccgttt
     1501 ctaaagcttc catatcattc gttgcaattt caagaactcg ggtaacagat tcaatgttaa
     1561 tcattttttg ttctaactca accattaaat cctcggtatt attgcttgcc gtaaagaata
     1621 acttcaagaa agtttgtgga atacctggca gattgcgttt ttccatgtat cgcttgatag
     1681 tatggagacg attgacataa acattggcct tttgacgtgc attaatatca tctttctcaa
     1741 tttgtgtcag gcgctcacta actgaaattt gctcatcttc aatatctttt agttgagttt
     1801 gtaaatcctc aagattttct tcaagaactg aataagcttg ggttggttct tcttgatttg
     1861 aagttacctc aacaatagct gcctcaaaac tctctaattc tgtctgaata cgacgaacat
     1921 ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa acgtgcaata tcttctccca
     1981 ataaagtatt attctctttc atatgttgaa ggtaagttgg aagagttgca agtagatttt
     2041 ccactacttt ctgagcagca atttctcgag taaaaatatc atacaaggca ttgatttcct
     2101 cttgtgcctg tccattctca tattcggcat tatccaattc caactgacga atattctctt
     2161 ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc ttcaatatcc gtttcaacaa
     2221 aatgataatt agcatcaatt agtttacgat aaccggcttc caaatcctgt aattgatctg
     2281 gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg atccacaata tgacttaagg
     2341 ccaaaatgtg attttctgta ttatccaaaa tcactgcggc ttccacaggg tcacccgatg
     2401 aattcaaggt tacaaattgt gaaaattcag attggatatt ttctaattgt ttttcaattt
     2461 catccaaggc ttgaccatac tgttctgaat tttcagcaac tctatgctga agttcctcaa
     2521 ataaatccaa agcatgaaga acacgaccac tatttttaga ttcttgcttc tctaagtctg
     2581 ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa agtaatttga ctctcaattt
     2641 ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg gttatagcct tctgcttcaa
     2701 agagattatt ttcaatatcg gcaaaagagt tgagagataa atcgacccat ttttgattcc
     2761 attcacggaa agccacttga ctttgtccaa tcaagtgcat attttttaca gcttctactt
     2821 catcatttac tggaagattg tatagttctt cttttctttc ttctagcgcc tctaatctcc
     2881 cctcgtttcg cttacgtaga aagattgcca ctacataagc cagaactaaa atgactgcaa
     2941 ttgcaaccat taaataaatt agttgtccat tagacatatc aaactccttt tttacttgaa
     3001 acaatcgtaa cgattatatc atatttttta gaccaaggga actacttctc cctatttttt
     3061 agacatcaag tgtactatag acagcatttt cttcgataaa ctcacgacga ggctctactc
     3121 gatcccccat caacatatca aagattttat ctgcttctgc agcatcatct acagaaactc
     3181 tagccatcaa gcgatgttcg ggatccatgg ttgtttccca cagctgatga tcgtccattt
     3241 cacctagccc cttataacgc tgaatagtcg gtttggtacg accttcacta taacgggcta
     3301 aagcttcttg gagtttgatt tcttgatctg cacccggctg gatatattct ttaatctcgc
     3361 ttccaacctt gacaccatag attggtggtt gggcaatata aacataacca gcttctagga
     3421 ttggtttcat ataacgataa atcaaggtta aaagaagggt acgaatatgg gctccatcga
     3481 catcggcatc ggtcatcaaa acgagttttt ggtaacgggc tttcgaaaca tcaaattctg
     3541 cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat ttcttcgtta gctagaatct
     3601 tatccatact tgctttttca acgttcaaaa tcttaccgcg aattggaagg atagcctgaa
     3661 actcacggtt acgaccagat ttggctgatc caccagctga gtctccttcg acgatgaaga
     3721 gttctgtttc agcagggtta ttagaagaac agtctgctag tttccctgga aggttggaaa
     3781 tttccaaacc agatttttta cgtgtgactt cacgcgcacg cttggcagcc acacgagcct
     3841 tggcagctaa aatccctttt tccacgatac gtttggcaat ctgtggattt tccatgagga
     3901 aatcggagaa ggcttcactg aagaggcgat tggtaatctt gaccacttcg ctatttccca
     3961 atttggtctt ggtttgtcct tcaaactgtg gatttgggtg tttaactgag ataactgcag
     4021 ttaagccttc gcgaacatct tcccctgtta aattatcttc attgtctttc agtaacttat
     4081 tcttacgagc ataatcattg ataacacgag tcagtgacgt acggaaacct tgttcatgcg
     4141 ttccaccttc atgggtatga atattattgg cgaaactcat gacattttca tggtaacctg
     4201 ttgtgtactg catggctacc tcaactgtga tatcatccat ctcaccgtct gtatagattg
     4261 gtgtatcaaa gattacatcc ttgttctcgt tgatatattc aacgtaacta gcaatcccac
     4321 cttcataatg ataatgcttg gtttgttcca aaccttggcg cttatcagtg atagaaattt
     4381 gaagaccgcg atttagaaag gccaactctt gaatccgttt atttaattta tcaaaatcaa
     4441 aggttgttgt ttcagtaaag atttttgggt ccggtatgaa gtgaaccgtt gttccagttc
     4501 tatctgtatc cccaaccacc tcaaggtctg caacaacatg accacgacgg tattcttggt
     4561 aatgaatctt accgttttta tggacatgaa cgtctaattg agtggaaagg gcattaacta
     4621 ctgacgaccc caccccgtga agaccacctg aaaccttgta tccaccaccg ccgaactttc
     4681 ctccagcgtg aaggactgta aagacggtct caacagcagg acggcctgtt ttttcctgaa
     4741 tatcgactgg gataccacgc ccatcatcca caacagtaat cgaatcatct ggctcaataa
     4801 aaacttgaat atggctggca aatcctgcca aggcctcgtc aattgagtta tcaacaattt
     4861 cccagactag atggtgaaga ccttcttttg aggttgatcc aatgtacatc cctggacgca
     4921 tacgaacagc ctctaagccc tctaaaactt gaatttgact ggcatcataa tcctgtgcct
     4981 gcagattttt gatttcttct gtcatcttat tcctttttct tatatgtcta atacttgtct
     5041 taaattcacg atactggtaa acaagtgggt atcaagtcct gcagcttgac ctgcttcgat
     5101 atcaatcggc cgatcaccaa tgacaagacc agagctaatc tgatactttt ctcttaaata
     5161 aagcatggat tcgggatttg gctttctctt aaagcctgag ctagaagtca ccacttctgt
     5221 aaaataagct gctatagagg ttttttctaa aatttccaaa acctgatcat ttcgatgaga
     5281 gaccaaaaaa tgacggccac cttgatttga aatgtcttcc aataggtcag aaactccttc
     5341 aaataaaatc gggtgttcaa gctctctggc ttcattttcc ttgtactttt ctaaaaaatt
     5401 ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta gaaaccttta aagcttgata
     5461 gacactgtca tggtcttgtg tgataccata cagtgccaat gtttcaacaa atgcagctgt
     5521 tgaagtttca taattatcca gtaaagttcc acctaaatcc cagatgtaat cgtgatattt
     5581 catacccttc attatagcat ttttttatga aaaaagcgat gatttccctg agttttccac
     5641 ataaaaaacg agagaataac tcccgtttta ctgattttta ccaaagacat cttgatagag
     5701 cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc tccaccaact cgtaggcaaa
     5761 ggcaagggct gttgaaggac cccgactggt tgtcaactga ccatctacca ctactgtttc
     5821 cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg tcataacaag tgtatcgctt
     5881 atttttcaat atctctgctt gattgagggc aattggtgcc gcacaaatgg ctgctagttt
     5941 cttcccttct tgctcgaagc tttgcaattc ttgaatcaag gtctgattat cacgtaaatg
     6001 tgcagaacca ggcatacctc caggaagaac aatcatatca tagtctgata aatctccatc
     6061 aaagacatga tctgctctta cttggattgc atgcgaaccc gttacttgct cttcaaaacc
     6121 aaccatatca catgtgatat tggctcgacg caagacatct acaactgtca aggcttcaat
     6181 ttcttcaaag ccctgagcta acataactgc tacttttacc atgattttct ccttatttaa
     6241 tttgttttaa tacaaccttt ttccgtttga atgggacttt tccgctcttt tcttcagcta
     6301 gattagtctg gtaactcatc gataaaagca aaccaacacc aatcagatta ctgataatag
     6361 ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt caaaggaagt agtccagtca
     6421 cagcaccgat attctcaaag atgtggaaga gcaacatcat aatcaaacct gtggaaatat
     6481 aagtgtagaa ctggttattt gatttaagag taatcttcaa catacggtaa atcaacatga
     6541 gatagagggc aataaccagg acagagccaa taaagccaaa atcttctgca ataaccgtaa
     6601 aaatcatatc tgactctcga actgggataa gcagattcga agcattaaat ccctgaccaa
     6661 ataagccacc actcccaatg gcaatctgcc cttgagcctg ctggtaagtc gttgtttggg
     6721 caaactcaaa gggattgagc caagccaaaa tccgattgat ttggtaggtc ggcattccaa
     6781 tctggtgaag aaaagctcgt ccgtccttgc taataaagat agctaagaaa ccagcaactc
     6841 ctgttacagc agtcacaaat actgggataa taattttcca agaaacccct gataataaaa
     6901 cgattcctga gaaaatggct acaaaaacca aagccgtccc caagtcactt tgaagtgcta
     6961 aaagaactag gactggaatg gtaaagagaa tcatccagaa aattaacaaa aagtccagcg
     7021 gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg gacaatgaca cgagccaaca
     7081 tgaggatata ggatatcttc ataaattctg acggttgaaa tagggtaatt ccatttattg
     7141 atacccagtt tttggcaccc gttgatgcaa ctaagcttgg attataaaat acaatcggca
     7201 agatcataag tcccaagcct aaaatatata gaaagggggt caccttccaa agaaattctg
     7261 tattaaagag catgaccaca aaaccaatca caagccccaa ggcaatccag gcgacctgct
     7321 gccctaaaat gggcagaata ttattgggat aatcatgact aacggctata tagatagcca
     7381 ccacaccgat gaccagtaga aaaaatactg gcaagagcaa actataatcg actctagagt
     7441 cgagagaacg tttcatatat actaacctta tactttcata caatactatt tatcaaagtt
     7501 cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg agagatggtt ttaacagttg
     7561 cttcttctgc tagagatgct actatttgtt tgccgtatcg ttttccgatg attctcctat
     7621 ccaaaataag agttaaggaa cgttggtatt cacgtctcat acttcttccc aaagcctgtt
     7681 ttaaacgaat aatggccatt ggcaattgat aatcataaaa ggcatttttc ccttcttgat
     7741 tcagttcttg attaatcttt ttcgtcaagg gttcttgagg attttggaaa ggaagcctcg
     7801 gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac tccctcccag aaacttgctg
     7861 caccaagcaa gatttgttgt tcaccttttt caaagcgttt ctttagctga tgaacatccc
     7921 catttttata ctgggccaag tggctaactg taagtaaatc cgatactgct agaagcatgt
     7981 ctttagcggt aaagagaacc aaaatcggtt gctggaaagc ttgaatttcc actagtaaag
     8041 cagctacctc tctggcatag acttctaagg aggtttctgt taccagaggg aaatctttga
     8101 ccataaccac ttcttgttcc tgttttcccc gagattcaat cttgacaaat ttggcttcag
     8161 gatagcctaa aaggtctgcc aaagaaaccc tctgactaat ctcaagagta gccgatactc
     8221 ccaagacttg gcaactctca ggaagtaaag aggacagaag agtacggcat gattttgtag
     8281 aagaaatctg aattttcttt tgactcgttt cagttacttc aagccagtaa tcaccttcag
     8341 ctgtaaaata gcgaaccagc tcatcaaagc cttctacttc taattctgaa aaatactgat
     8401 ggagattgtc cagagaatct aagatatttt tcctagattt accagactga aattgttcta
     8461 tcaagtagag acactcaaag cgaatacttt ctagtatccg ttgttgaacc ctgttttctt
     8521 ctcctactaa agccttatca attaaatcga taatagattg tatatcgtag gtctcttgaa
     8581 gcagattttc tagagctaac aaaatctttt ggacttcatc aataatcagt aaacggtcac
     8641 tgacaaattc aggattatct tcaagtctgg tcacgagata ggcatgatta gtcactaaaa
     8701 gcttgcaagt ctctgccctt tcttgactac gtttccaaaa atcttccgtc acaaataagc
     8761 tctgggatga taaattccca tcatgacgaa ggtctgctag aaaatgttgg taacggtaga
     8821 gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt aagccagacc aagacttgca
     8881 ttttaaagcg tctaaataag cgattttcat catttttctg caaggaatga taaaaggcat
     8941 ccaacttcag ataattttgt ggtcccttta agctatgaat atctgtatgg aacacttcct
     9001 tgaggcgttt accttcttct tccatgattt gattttgaag aatctttgtc ggaacactaa
     9061 gaacaatttg tcgctctttg gattgagata aagcgggtaa gagatagcca taggttttcc
     9121 caatccctgt cggcgcttga atcagagaga caggttcatc tttcaatagc aagccaacct
     9181 ctttagcaaa actttcttgt tcctccctca cttcaaggtt caacagagaa atatttttag
     9241 aaaagtcttg agatagtttt cgtggctcca gagaagctgc cgttttctta aaatacagac
     9301 cttgaacttg gaccaagtct ggagaactca ggatagattg gttgcgataa gtttcctcaa
     9361 taaccaggta ggactcatat aggagagcgt cagccatttc cagcaagcgt tccaagagac
     9421 ctttaggaag ctgggtcatc ttttcccgta aaaaaagaag taattctgct gtagcttggg
     9481 catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc taattctcga cacaaaatcg
     9541 gcaagctata tttttccagt tcagggaaaa agacctgggc caattcgacc gtatcaacac
     9601 gagggtttct tagctcatag ccttcaaaaa ataaattttc cgccaagaga ttagcatcaa
     9661 actgaacatt atgggctaca aaaatcccat cctccaccaa gtcaaatatt tttctggcaa
     9721 cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt caatcctgtc agttctttga
     9781 tatgagcatc caagggttca tgtggattga catccgtcgt atagtgatcg acgatttctc
     9841 cgtcctcaat cacgacaatt cccacttgga taattttagc cttactacct gtgctagttg
     9901 cctctaaatc caccacaaca tagcgattac caatcgtttt cattataaaa aattatacca
     9961 aaaaaagggc catttggcac ctgcaaacat aggataattt tcaaactcaa gaacactact
    10021 tttcttaaaa atcccatcga atagagcatt ctagcctgct gatttttgta aaatagtgaa
    10081 gaaatacgaa aaattgagag aagaaatgtt tgcatcttcc ttctctcaac gaatcatgta
    10141 ttttatctaa ccatgtcagg atcgtagtca taaaatcctg tatcaaggaa acggttttta
    10201 gggtgaagtt tacgaacttc ttcatccagc aagaaggctt gatcatggct aaagttgccc
    10261 tcttgaatca agttacgcgc ttggataaag agttttgcaa tctcaacaaa gttctgacca
    10321 ggagtgtaga gcccttctag tttccagcga gtaaagccat gctccaccaa ttctgttaat
    10381 ttgatcatca aatcaaggtc attgttggca aagatatggg tcccatgatt atcttcaaaa
    10441 atggaatagt ggctctctgg atcacttggc tcagccaaga agaggtcacg tttatgcgtc
    10501 ttttcatcat cgatatgtgt aaagttatag tagttttgca agagtggacg tttagaatga
    10561 tggatgacgc tagcaccgta aaccaaaact tcagcaggaa tttccaaaat ctctggcatt
    10621 ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag cctcagatgc gccagccttt
    10681 tgtccccaga agttaatctg acgactgcta gttaccatgg ttgaagcatc gtagatggtc
    10741 ttaaatgaat aaccatcgcg gttaactacg taaaagacgc ctgcatcccc aatcgtaata
    10801 tagtctgtct tgatttcttc caagaagtct aagaaaggct tgatacggtc catcatatct
    10861 tggtgcatga gagcattgac cgcaacgatc aattccttac cagcatcatg aaccaactta
    10921 gcgatttcac gtaattggtc ataactaaag gtcgttggca gacgaaggcc aaaatctttc
    10981 tcaccgacat agatacggtc tacgccagct tcgagtagtt gttcaacttg ttcaatactt
    11041 tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa ttataccaca tttctccaaa
    11101 atcatccatg ctttgaaaat gaaaagggac tttattcttt ttgtaacctt tctgtaataa
    11161 ttctctgccg aaaaaatgat aaaataggta tgtactgtta aggagagaat tatgcccgta
    11221 agaaaattac aatcctatga ggtagactat caagaagaat taaaccagca gcttcctcat
    11281 taccaagctt atacacctga agcgcaatct gatgccaatc tcaaggaaat cctatttttt
    11341 attaatatcg ctgttttttg tatctgtatt gctatcttta gttttatctt tttagcatta
    11401 aaattatcaa ctgctcttgc cttttccgca gcaatcggat tcagcttact tgttttaaaa
    11461 gttcaacggt ctattatcaa acgaaaacgt agaagataaa tccaaaatat cgccccagtt
    11521 ggagcgatat ttttattttt tctttttaga tggtgtctga atggcaatct tcacttcaaa
    11581 gattgttccc ttgggtttat tatctttgac agtaacagtt ccttttagag catctacaat
    11641 ttgcttggct agggataatc ctaaaccaaa accacctttt tgccgggttc tagctttgtc
    11701 tactcgataa aaacggtcaa aaattttctt tttatcttct gtcgaaatac cgattccatt
    11761 atcagaaaca agtaaataaa gattgcgatc ggtcgccgag ataagaaaat caatttcacc
    11821 atcctcctca gtatacttga cggcattatc gaaaagaatg gtcatcagtt gtttcagaag
    11881 aagctgatct gtgacaattg ttcgatggat acgattttca aaacggaaga cacgattatt
    11941 ttccgaagca atcatctcgt agtttgtgaa agttgtatta aaaaagctag ttggaacttc
    12001 tgcaagctcc ggcttaatcc catcatctct ccgagctaag ttcagcaagc ttgtcgttaa
    12061 aaaacgcata tttcggactt cttccaaact cgatgcaatg ctttcgctca catccataat
    12121 ggtagcttct ggcttacgaa aaagggtctc taagcgattt tgcaaaactg cgagtggagt
    12181 tcgtaactca tgactggcat tttccacaaa agactgttgc ttctgcatac tctcaagcag
    12241 gggcctaaca ctgaccctag ctagatagag actggcaagt aaagacaaaa tccagaaact
    12301 agccatcacg accacaatca attgctcatg cttttgacta gcctgctcca actgactggt
    12361 attaatcaag acagcagcat acttgatatt ggttgaaacc gaactaatat tggtctccat
    12421 caaaatcaca cgatagattt cttcctgccc atagctatta aaaacctgaa tctggtagat
    12481 atgtcctagt tctttctttt ctaacttaat cttatccaag cccaaaaatc gatttccaga
    12541 aagaagttga gtaaagtctt tatcaaagag aatgacttcc gtattggaac tgacattagg
    12601 ttttatttct actttactag cgtccgccct agcattttct aaatctttaa tctcttctgt
    12661 tgccctattt atagccagct gaataactgc ttgaggattt tcactcagtc catgaagctt
    12721 atcgtccacc gaagtataaa gactcgaatg catgacttgt aaaataatca gagtcattgt
    12781 agaaaaaatc agggtgaaga caccgaagtt gcggataaaa taactaaagt catccgcata
    12841 ccatgttttt ttaagtttac tgaacatctt ttaaaagata cccaacacta cgcaaggttt
    12901 gcaaattctc tgcaaaagtg gttcccttta atttcttacg gacttttgaa acatagactt
    12961 cgacaaccga aatcgttgta tcactatcaa atccccatag acggtcaaaa atctgcgtct
    13021 taggcaaaat cacattttga ttttgaagga aataaactag taaatcgaac tctttcccca
    13081 gcaattcgac aggagtatct tcaactttaa cggtattggt tgataaatta accacgatat
    13141 tcccataagt caaggtgttt tcattaaact tccctgaacg tttgagaagg gcctgaatcc
    13201 gcattttaag ttcttctagg tagaaaggtt tggtcagata atcatccgct cccagttcaa
    13261 atccatgtcc cttgtcatcc aaactttcct tggcagtcat aatcagaact ggtgtcgtaa
    13321 ttcccttttc acgcaattct tttaagactt ggaaaccatt tttttctggc aacatcaaat
    13381 ccagcaaaat caagtcatag acaccactct cagcttcgta gagaccttct tctccatcaa
    13441 atacctgcat aacatccgca aaatcgtcta aaaagtcaaa tactgaattt gacagaccta
    13501 ggtcatcctc aaccaataag atttttatca tgagaaactc ctccttatta aaactattat
    13561 accaaatttg ccttaaaaaa aactcaactc tctgcatttt acatgagata gctgagtttt
    13621 ctttttattt taggcttatt tatgcatttc cgtattgaag aacaactgct tcgactgcag
    13681 ctttttcacg gctaatcaag tcaacacgcg ctgcaatttc cttgattccc ataccgatgt
    13741 tacggctaag agcaaggtca gaaagttgcg gttcaaagaa ctccttgtat tccgccaagc
    13801 gttgctgagt cttaaataca tgagcaggaa ggataacaaa gctatcaaag ctcatatctc
    13861 ctccaagggc tgccttaatc caagcccagt tttcacgcgc ccaagaccaa gctgttttct
    13921 gagttgcttg atgagctagg aattggtaat accaagcaga caagtcctgt ggtttgacca
    13981 caaatttgtc cttccaagaa gtaatcaagt tttggatatt atccgcatct gtactgtatg
    14041 caagagctgc tgccaactgg cgtttaaaga cagcatctgt tgcgtgagta taagtatcaa
    14101 gataaagtgc taacaagtct ttagtctcat gatgtttcat ctcattaatc agaacttgtg
    14161 aacgaatagc tgctgggagt cctgcaagat tctccttgtg tgttgcgaag atttggctag
    14221 cgacttgact agcttctgca tcatttgagc gaatcatcat cgaaacagcc agctgacgaa
    14281 ccaattcatc ctcatctgat tctccgtctt tagcttcaaa accaagacgg tcatagttat
    14341 gacgagccaa tttagcaacc agtcctttga aggctgtttc agcatccgtt ccttcatcaa
    14401 taaagcgctc aagggctgaa atcacttgag aaacagctga aaccaccaga taagactctt
    14461 ccttagcaag tttatcaaga actggaagca agtctgcata agaaatgtgc cctgcctcag
    14521 ccaacaaacg acgttcttga acaatttgca gtttgcttgt gttatcaagt gtctctagct
    14581 cagcaagaac agcttctaac aagtctcctt gatagtcggt aatatagtgg gcagtatttt
    14641 cagtgttgag acgaagagct ccttcatttt cagcaagaag agctgcgtag ccagggattt
    14701 cgatactttc agtttcgagt gtatcaggca agcctttcca gttgctattg aggggcacca
    14761 cccagagacg gttcttgtct tcgttctcac cgatgaagaa ttgtttttgt gaaatcttca
    14821 agacatcatt ttcaactttg acagtaagaa ctgggtaacc aggctgttcc aaccaagaat
    14881 ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag ggcatcccaa aggtcactac
    14941 caatggtatt gctgtattgg tgtttttcaa agtaggcgtg caaaccttta gcaaaatcag
    15001 catctcctag ccaacggcga agcatgtgca tgagacggct tcctttggca tagacgatag
    15061 cgccgtcaaa gagtgtattg atttcatctg gatgtttaac ttcgacgtgg acagactgaa
    15121 cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc acctgtttgg aaatcttcaa
    15181 agatattcca gcttggttcg atggtatcca cacagacgta ttccatcata ttagcgaaac
    15241 tttcattgag ccaaaggtca tcccaccatt tcatagtcac gaggttccca aaccattggt
    15301 gagccaattc atgggccaca acaagggcaa cttgttgacg gctagcaaat gtagagttct
    15361 catcgacaac caagtaaact tcacggtagg tcacaagacc ccagttttcc atagcaccag
    15421 ctgagaagtc aggaagagcg atgtggagag attgaggaat tgggtactta actccatagt
    15481 aatcttcgta aaactcgata gagcaaacag cgatatccag tgagaaatca agatttgaaa
    15541 gtggatgtgc tttggttgag tagacaccta ccagggtacc atttttagtt ttagcggtca
    15601 ccccttgcaa atcaccagca acaaaggcca acaagtaaga agacatgcga ggtgttgtct
    15661 caaacttcca gatacctgtt tccttacggt tttcaacatc gatttctggc atgtttgaca
    15721 aggccaattc accttctgct tggtcaaagc gaagagagag gtcaaaagtt gctttggctt
    15781 caggctcatc cacacatggg aaagcttcgc gcgcaaaatg gctctcgaac tgagtagaca
    15841 agacctcctt cttgactcca tcaactgtat aataagaagg gtaaatccct gtcatgttgt
    15901 ctgtaatttt accagaaaag gcaagaacca attcaacttg accagcctca gccaattcga
    15961 tatgaagggc ttcattgtca tggtcaactg taaatggacg agcttgacct gcaacttcta
    16021 cagaggtgat ttccaagtct ttttggtgga gggagatgcg gtcactctgt gcttgaccag
    16081 tgatggtcac tttcccagaa aaagtcttgg tctcacgact caaatctaaa aataaatcat
    16141 aatgttcagg aacaaattgc ttaataaaat gttcaactgc ttgcatagtt ttctcctatt
    16201 ctaagtttaa gagctgctgc tcttcttcaa acaaacttat tatatcatat tttgcttgag
    16261 aaaaaaggat tggacagcga tttccaaaac tttcttcctt ctaatagtct acagtgggta
    16321 gaccttgcga aattcttcta aaacaacctt gctttcaggt gtaaaagtca gccctacttc
    16381 acccaaacag tctgtgaaaa agtcctcatg aacctggcgc caataggcaa gtgatttgtc
    16441 tccctcacct tccttaaagg catgttgcgc agaaacttga ttgaagagtt caacagaaac
    16501 ctttgtaatt tcgacaatgc agacagactg attttgacta tctaaaatga catcgaaggt
    16561 ccctacttgg ggaagtggtt cgtcttctag tacatagagg tcataggctg atgctgttgc
    16621 tgtcttttct cctttaaaca ccaaatccgc taaaaggtct ggttcaactc caaaagccca
    16681 agcatcgatt tcatctccga tcaaaggatt gatttgtttg tatttattcc acatttcttg
    16741 cggtgtcatg ggtgctcctt tgtaattttt tactttcttc ttttatgtgt ttaagatgat
    16801 ctggatggtc aatctctaaa tcaaaaatct ctggaataga actgtagtgg ataatgcact
    16861 tgatacccaa ctgattcatt ttttgtatga aagaagtatt cagatagcct gctacagcaa
    16921 aattaatctt gttctttctt gctttatcct gcatatctct tagcatatct aacattattg
    16981 gactttccat atcatgccat tgactgtttc tcatagtcgc aaaaacaaag gaagtcaaat
    17041 cattcattcc aactacaatc tttgaaatgc ccgtttccag tatactagat aagtcaaaat
    17101 acgctgacgg taattcaatc atcgttccga ctttcccagt aaagccatac tgacgcaata
    17161 ctgtaatagc ttgttttaat tggtcggcat cattgacaaa aggaaaaata agagacagat
    17221 tgggattggt ttgataaact tctgtaacga catgtgcttc agcctgaaat tcatccaaac
    17281 acgccagtaa acgcctggtt cctctatagc caaacaaggg atgcccttcg tcaaaaaact
    17341 ctctagtccc cgctaaacaa ttggcttctg tattcgttaa ttcagaaaaa cgataccaaa
    17401 cttccttatc taagtaaaag gagcaaatag tatcaagata atcttttaca aattgctgac
    17461 aacttgctaa tatgatgttt tgattaagct ctctcaacaa atattcccca cgaatcatgc
    17521 cgacgtggtg aaatagttga ggataaattt tttcaagaat tttttcgcca ctaagggcaa
    17581 gttgatttct catcattcac cttccaattc atgtaagaag tcttgtccag ttctggaaat
    17641 cctaataatt cagacttaac cttcaagact aatggcgatg cattttcttc tgtaatctct
    17701 tgaatatcca tccaaatata tccaagtgaa tcattcgcac catcagacac agcttccgaa
    17761 gtcgtaactt gaggtgcact ctcattcatt tcaacatcat acaaggccat gacatggtga
    17821 accataaaat ttgttaactc ttctctgacg aaaacatcgt agattcgagg attagagtag
    17881 cttctaacag taaatcccgt ctcttccata acttctctag tcagcgtttc cgtcagtcct
    17941 tcaccaagtt gctgactgcc tccaggtaga tcataccgat gttgataagg gcctctcgtt
    18001 ttttcaatgc agagtaactt cccattttct aagcaaacag cgtagactcc aaagtgtttt
    18061 ttgatttcca tccaactcct cctacttcaa agaccagcca ccatctattg tcaagatttg
    18121 tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga ctaagctctg ctatttcctc
    18181 tggctcaatc cagcgcttga ttggggtttc actagccacc cagtcagcca aaccacctgg
    18241 ttcaaaatcc gcagcggtca tagctgtctt gactgctcct ggagcgatac caaagacctg
    18301 aatcccagct tcagcatagt ctagagccaa ctgcttggtg aagccagcca aggcatgctt
    18361 ggatgaagta taggcgtgac cacctccacc tgctaggcta gaagcaatgg aacacatatt
    18421 gatgatgatt ccctttttat tttccagcat ttgtgtcaaa taataccgag tcaactctac
    18481 tggaataatg tagttgattt caaaaatctc ttgaatgtcc tgcgccgttt gttccaacag
    18541 tggtttgtaa tcatccaaaa ctccagcagt attacacaaa acatccacct gagggcacca
    18601 gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt aaaaagtgaa aatcaccctc
    18661 taagagtggc ttttcacctt ggtcaactcc ataaacttga tagcccttct ctaaaaagag
    18721 gcgagcttga gccaatccga tccctgaact cactcctgta atgagtacac gtttagtcat
    18781 gcacttctac ccaatccgtt gccaaaacat cacaaactgt cgggctccac atggaaaaac
    18841 cttctccttc gccagaaacg ttgattagga aataaggtgt catttcaagt gcaagcccat
    18901 tttgctcgat ggtatcaaag agttggacat agttttccgc acctccccaa ccagttcgta
    18961 catattttct cttagccttt aacccaggca ggatctcttc aaatgtcatg tttttctcct
    19021 ttaattctac attcttcatt taattatagc aaaaaaccgc tttatacggc tttttgaatg
    19081 tgagttattc aaacctgcta ctacttacgg caaattattc cctgcagcaa gataaatttc
    19141 ataccattct tttcttgtta agctaaagtt tgccgctcgg ctaacttctc tcaagtgctt
    19201 aggatttgtt gtacctacga ctgcctgcat ttttgctgga taacgcaata tccaagaaat
    19261 ggcaatagtt gaagaggtta ctccatattt aatagctaaa cgatcaagta cttgatttaa
    19321 agcttgaaat ttctcatttc caacaaaatt tcctttaaaa tacccgaatt gtaagacaga
    19381 ccatgcttga atgaccacat cgtgtaattg gcaatattca aaaatgctgc catctcgcat
    19441 agctgcttga ctatcttcca tattaacatg aaaagctgat tcaaatcctg gagtaaaagc
    19501 cgcactcaat tgtagctgat taacagctaa cggctgcttg acatcttttt taagcaactc
    19561 catcatcata ggattttgat tagaaactcc aaaatctcga actttacctt gtttataaag
    19621 gagatcaaag gcttctgcta cttggtcaga ttccatcaaa gcatctggtc gatgaaggag
    19681 caagctatct agatgatcaa tcttcaatct ttgcaaaata ccgtctactg attttataat
    19741 atagtcctta gaaaaatcaa aataggtaaa ttcttcaatg cgaatgccac atttggactg
    19801 aatccacatc ttttctctta aatctggacg attttttagg acaagaccta acagttcttc
    19861 acaacgacca cgaccataaa tatcagccaa gtcgaaggca ttgattccaa cagaaagtgc
    19921 tgtttctaca agctcttcaa cttcttttac agatttatct tttattctca tcattccgag
    19981 aacaatttct gataattctt tgtcatcttg accaagagtt atgtatctca tcaaattttt
    20041 ctcctttaat ttctaacatt cttcccttca ttataacaaa aaaccgcttt gcaacgactt
    20101 tttgactata cttcactcca ttttatcttc ttaaacccac ggaacaagac aaagattcca
    20161 ataaagagga cagctaaagg aataactttt gtaaggaaaa catttgaaat tcccatccac
    20221 tcataataac ggagcagaga acccaccaca agatgggcaa taatcatact gacaaatgga
    20281 cgaaagaccg cttctttcca attccaaata ccgataacta gcgaaatcgt aaagatagac
    20341 aaactatccc agggagccgg aatataaaag gctccttctt gtatgaagct tgccattcct
    20401 acatatccta aaacaactag aagaactata gtcccaacaa caatgtaagt gccaattttc
    20461 attttaggag aatcttggac taaacttctt cgtaaaattg tggccacaag tccaaatcca
    20521 atcagaaaaa taagaagttg ccctaaaaat gtgagcaaat tgactgttaa gagaggacct
    20581 ttagaaaaat cacttagtag ttgataataa cgtaataccg ccaggacaag aattggcgtc
    20641 aaaagggact ctttgataga actgcgaggt gctcccttga gaatctcttt cattattttt
    20701 ttaggattct tacctagata atcctctgca ctcatgccat ctcgttctgc ttctgagaaa
    20761 tctagcatca tcaaatagat ctgctctctg agatagtctt catcatagag aaatccagca
    20821 agattaaaac tttcccacaa ctcctcaaaa tacttttgat tctcctcaga aaactcatgt
    20881 agcaaagcgc ttgtttcttc gtaatacttc attttcttca tggtttaacc cccattctta
    20941 atcccttcta ctttttgact caaatcgtcc cattgttgcc aaaagactga gacacgctct
    21001 tctccttctt tcattaatga aaaatacttc cgatctggac catctggcga cgggcgcatg
    21061 tcgcctctta tccattgatt tttttctaac ttttgcaaca aaggataaat agttcctgga
    21121 acgatagtat caaatccagc ctctcgcaaa gtctgaacca actcataacc ataccgctct
    21181 ttttgaccaa tcatatccaa gacacaacct tcaagaacac cttttaatag ctgagtttct
    21241 ttcatcactt ctcccttcta atctattttg taatacctac tagtaacttc acctatagta
    21301 tattacttct acactagttt gtaaagcata atagttaata ctcttcgaaa atctcttcaa
    21361 accacgtcag cgtcgcccta ccgtatgtat ggtt
//
LOCUS       DAVHIU010000025        19568 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVHIU010000025 DAVHIU010000000
VERSION     DAVHIU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.027"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            216..2003
                     /locus_tag="VVN06_000788"
     CDS             216..2003
                     /locus_tag="VVN06_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5562370.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2000..2476
                     /locus_tag="VVN06_000789"
     CDS             2000..2476
                     /locus_tag="VVN06_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU5562371.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2504..3409
                     /locus_tag="VVN06_000790"
     CDS             2504..3409
                     /locus_tag="VVN06_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU5562372.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3396..4211
                     /locus_tag="VVN06_000791"
     CDS             3396..4211
                     /locus_tag="VVN06_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5562373.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4218..4622
                     /locus_tag="VVN06_000792"
     CDS             4218..4622
                     /locus_tag="VVN06_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5562374.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4916..6082
                     /locus_tag="VVN06_000793"
     CDS             4916..6082
                     /locus_tag="VVN06_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5562375.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6198..7235
                     /locus_tag="VVN06_000794"
     CDS             6198..7235
                     /locus_tag="VVN06_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU5562376.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYET
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7442..8296
                     /locus_tag="VVN06_000795"
     CDS             7442..8296
                     /locus_tag="VVN06_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5562377.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8446..8703
                     /locus_tag="VVN06_000796"
     CDS             8446..8703
                     /locus_tag="VVN06_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU5562378.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8916..8999)
                     /locus_tag="VVN06_000797"
                     /pseudo
     CDS             complement(8916..8999)
                     /locus_tag="VVN06_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(9033..9125)
                     /locus_tag="VVN06_000798"
                     /pseudo
     CDS             complement(9033..9125)
                     /locus_tag="VVN06_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9161..9457)
                     /locus_tag="VVN06_000799"
     CDS             complement(9161..9457)
                     /locus_tag="VVN06_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5562379.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(9447..9608)
                     /locus_tag="VVN06_000800"
     CDS             complement(9447..9608)
                     /locus_tag="VVN06_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU5562380.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9678..10442
                     /locus_tag="VVN06_000801"
     CDS             9678..10442
                     /locus_tag="VVN06_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU5562381.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11007..12386
                     /locus_tag="VVN06_000802"
     CDS             11007..12386
                     /locus_tag="VVN06_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU5562382.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12400..13065
                     /locus_tag="VVN06_000803"
     CDS             12400..13065
                     /locus_tag="VVN06_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5562383.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13200..13358
                     /locus_tag="VVN06_000804"
     CDS             13200..13358
                     /locus_tag="VVN06_000804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562384.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            13420..13959
                     /locus_tag="VVN06_000805"
     CDS             13420..13959
                     /locus_tag="VVN06_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5562385.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQKNKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTALLRASQF"
     gene            14026..14436
                     /locus_tag="VVN06_000806"
     CDS             14026..14436
                     /locus_tag="VVN06_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5562386.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14552..16669
                     /locus_tag="VVN06_000807"
     CDS             14552..16669
                     /locus_tag="VVN06_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEU5562387.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGEAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISK
                     ETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKAS
                     DKAPWSDNGEAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQT
                     YSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHN
                     HATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASI
                     GFLGLALAGLLGGLGLKNKKEEN"
     gene            16836..17534
                     /locus_tag="VVN06_000808"
     CDS             16836..17534
                     /locus_tag="VVN06_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5562388.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            17531..18583
                     /locus_tag="VVN06_000809"
     CDS             17531..18583
                     /locus_tag="VVN06_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU5562389.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            18778..19389
                     /gene="rpsD"
                     /locus_tag="VVN06_000810"
     CDS             18778..19389
                     /gene="rpsD"
                     /locus_tag="VVN06_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5562390.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT         5598 a         3747 c         4442 g         5781 t
ORIGIN      
        1 atgcattcta tacaaaatta ttttaggtac tatattccaa atttaaagag cgtttgaaat
       61 gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat gcagtaaata
      121 tcccgacaat ttctattgac aattcaaaca gattggttta taattaatat aacaacaaat
      181 gaaagcgcaa actttcgcgg tcggaaggta gttttatgac acgatttgag atacgagatg
      241 atttctatct cgatggaaaa tcatttaaga ttttatctgg tgccattcat tattttaggg
      301 ttcctccaga ggattggtat cattcgctct ataacttgaa ggctcttggt tttaatacgg
      361 tagagactta tgttgcttgg aatttacacg agcctcgtga aggtgagttt cattttgaag
      421 gtgatctgga tttagagaaa tttctccaaa tagcgcagga tttgggtctc tacgcaattg
      481 tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg tggcttacca gcttggctct
      541 tgaccaagaa catgcgaatt cgctcatccg acccagcata tatcgaggca gttggtcgct
      601 actatgatca gttattgcca agactggtgc ctcgtttgtt ggacaatggt ggcaatattc
      661 tcatgatgca ggttgaaaat gagtatggtt cttacggaga agataaggct tacctgagag
      721 cgattcgaca gctaatggaa gagtgtggcg taacctgtcc cctctttaca tcagatggtc
      781 catggcgagc tactctgaaa gctggaacct taattgaaga ggacctcttt gtaacaggaa
      841 actttggttc taaggcacct tacaactttt cgcagatgca ggaattcttt gatgaacatg
      901 gtaagaaatg gccactcatg tgtatggagt tctgggatgg ttggttcaat cgctggaaag
      961 aaccgattat cacacgggat cctaaggaat tggcagatgc agttcgagag gttttggaac
     1021 aaggatctat caatctttac atgttccacg gtggtacaaa ctttggtttc atgaatggtt
     1081 gctcggctcg aggaactttg gacctgccac aagttacgtc ttatgattac gatgcccttc
     1141 tggatgaaga aggaaatcca actgctaaat atcttgcagt caagaagatg atggcaacac
     1201 atttttcaga gtatccgcag ttggaaccac tctacaaaga gagtatggag ttggatgcta
     1261 ttccactagt tgaaaaagtt tctttgtttg aaaccttaga tagcttgtca agtcctgtag
     1321 aaagtctcta tcctcaaaag atggaggagc tgggacaaag ttatggctac ctactttatc
     1381 gaacagaaac aaactgggat gcagaagaag aaagacttcg tatcattgat ggtcgagata
     1441 gggcccagct gtatgtcgat ggtcagtggg ttaaaactca atatcagaca gagattgggg
     1501 aagatatttt ttatcaaggt aaaaagaaag ggctatctag gttagatatc ttgatagaaa
     1561 atatggggcg tgtcaactat gggcataagt tcttagcgga tacgcaacgt aagggaattc
     1621 ggacaggggt ctgtaaggat ctgcatttct tactaaactg gaaacactat ccactcccac
     1681 tagacaatcc tgagaaaatt gatttttcaa aaggatggac tcaaggacaa ccagcctttt
     1741 acgcttatga ctttacagtc gaagagccaa aagatactta cctagacttg tctgagtttg
     1801 gtaagggggt tgcctttgtc aatgggcaga atctaggacg tttttggaac gttggcccaa
     1861 ctctctcact ttatatccct catagctatc tcaaggaagg tgccaaccgc atcattatct
     1921 ttgaaacaga aggtcaatat aaagaagaga ttcatttaac tcgtaaacct acactaaaac
     1981 atataaaggg ggaaaactta tgacaattgt aggatgccgt attgatggac gtttgatcca
     2041 cggacaagta gccaatcttt gggctggaaa actaaatgtt tcacgcatta tggttgtaga
     2101 cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg aaacttgcga caccaccagg
     2161 tgtgaaattg agtattttgc cagttgagaa agctgcagcc aatattcttg ctggcaaata
     2221 cgatagccaa cgtctcttta tcgtggctcg taaaccagac cgcttccttg gtttggtaga
     2281 agcaggtgta ccacttgaaa cccttaatgt tgggaatatg tctcaaacac cagaaactcg
     2341 ttctattaca cgttctatca acgtagtaga caaggatgtg gaagacttcc acaaactggc
     2401 agaaaaaggt gttaaactta ctgctcagat ggttccaaat gatccaattt cagacttttt
     2461 gagcttatta aaataggaaa aaaattttta ggaggtcatt gttatgatac aatggtggca
     2521 aattttactt ctcactttgt actcagctta tcaaatctgt gatgagttga cgatcgtttc
     2581 atctgcaggt tcccctgtat ttgctggttt cattactggt ttaatcatgg gagatgtgac
     2641 tactggttta cttatcggtg gtaacttgca actgttcgtt cttggggttg gtaccttcgg
     2701 tggtgcttct cgtatcgacg caacttctgg tgcggttctt gcgacagcct tctctgtttc
     2761 acaaggaatt gatgcaccgc ttgccattac tacaatcgct gtaccagtag cagctctctt
     2821 gacttacttc gacgttcttg gtcgtatgac tactaccttc ttcgctcacc gtgtggatgc
     2881 tgcaatcgaa cgctttgact ataaaggtat tgaacgcaac tacttgcttg gtgcgattcc
     2941 gtgggctcta tctcgtgccc ttccagtctt ctttgccctt gcttttggtg gtgcctttgt
     3001 acaatcagta gtagacttcg ttgaagccta caaatgggtt gcagatggct tgacacttgc
     3061 aggacgtatg cttccaggtc ttggatttgc aatcttgctt cgttaccttc cagttaaacg
     3121 taaccttcac taccttgcta tgggatttgg tttgacagct atgttgactg ttctttactc
     3181 atatgtaaca ggtcttggtg gcgctgttgc tggtatcgta ggtactcttc ctgctgaagt
     3241 tgctgaaaaa attggtttcg tgaacaactt caaaggtttg tctatgattg gtatttctat
     3301 cgtaggtatt ttccttgcag tgcttcactt caaaaatagc caaaaagtag ctgtagcagc
     3361 accttctaca ccatcagaaa gtggggaaat cgaagatgac gaattctaat tacaaactta
     3421 caaaagaaga ttttaatcaa atcaacaaac gtagcttgtt tactttccaa ttaggttgga
     3481 actacgaacg tatgcaagct tctggttacc tttacatgat cttgcctcag ttgcgtaaaa
     3541 tgtatggtga tggaactcct gaattgaaag aaatgatgaa agttcatact caattcttca
     3601 atacttcacc attcttccat accattatcg ctggttttga ccttgccatg gaagaaaaag
     3661 atggtgtagg ttcaaaagac gccgttaacg gtatcaagac aggtttgatg ggaccattcg
     3721 ctcctcttgg ggatacaatc tttggttcac ttgtacctgc tatcatgggg tcagtcgcag
     3781 caactatggc tatcgctggc caaccttggg ggatcttcct ttggattgca gttgcagtag
     3841 cgtatgacat cttccgttgg aaacagttgg aatttgctta caaagaaggg gttaacctta
     3901 tcaacaacat gcaaagtacc ttgacagctt tgattgacgc tgcatctgta cttggtgtct
     3961 tcatgatggg tgctcttgta gcaacagtga ttaactttga aatttcttac aagttgccaa
     4021 tcggtgaaaa gatgattgat ttccaagaca tcttgaacca aatcttccca cgtttgcttc
     4081 cagcaatctt tactgccttt atcttctggt tgcttggtaa gaaaggtatg aactctacta
     4141 aagctatcgg tattattatc gtacttgctt tggctctttc tgcccttggt cactttgcac
     4201 ttggaatgta attccttatg actaaatcat taattttggt gagccatggt cgcttctgtg
     4261 aggagcttag aggtagcaca gaaatgatta tgggcccaca agacaacatt tacacagtag
     4321 ctcttcttcc agaagatggc ccagaagaat ttactgctaa atttgaagct gttattgaag
     4381 gattggatga tttcctagtc tttgcggatc ttctcggtgg gacaccttgt aatgtggtga
     4441 gtcgcttgat catggaaggt cgtgatattg acctttacgc agggatgaat cttccaatgg
     4501 tgattgaatt tatcaatgcg agccttacag gcgcagatgc ggactacaag agccgtgctg
     4561 cagaaagcat tgtgaaagtt aatgacctgt tagcgggctt cgatgatgac gaagatgaat
     4621 aatactcttc gaaaatctct tcaaactacg tcaacgtcgc cttgccgtag gtatatgtta
     4681 ctgacttcgt caatcttatc cggcaacctc aaaacggtgt tttgagctga cttcgtcagt
     4741 tctatctgca acctcaaagc agtgctttga gcagcctgcg gctagtttcc tattttagtt
     4801 ggaactcgat tcaattcatg tgacaacgtg aaaatcgtta gagcatttta tatagaatat
     4861 acatgggaat ggagcttact cccattccca tatttaatag aaaaagagga actcaatgct
     4921 acattataca aaagaagact tgctcgaatt gggtgcagaa atcactacgc gtgaaatcta
     4981 ccaacaacct gatgtatgga gagaagcttt tgaattttat caagcaaaac gtgaagaaat
     5041 tgcagccttc ctacaagaaa tcgctgataa acatgactat attaaggtta tcttgacagg
     5101 tgctgggact tctgcttatg tgggagacac cttgctacct tactttaagg aagtctatga
     5161 cgaacgcaaa tggaatttca atgctattgc gacaacagat atcgttgcca atccagcaac
     5221 ctatttgaaa aaagatgtgg caactgtcct tgtgtctttt gctcgtagtg ggaactcgcc
     5281 tgaaagtgtg gcgactgttg atttggccaa atccttggtg gatgagcttt atcaagtgac
     5341 gattacttgt gcagcagatg gtaaattggc tcttcaagct cacggtgatg accgtaatct
     5401 cttgctcttg caaccagctg tctctaatga tgctggattt gccatgactt ctagctttac
     5461 gtctatgatg ttgacagctc tcttggtctt tgatcctaca gaatttgctg ttaagtctga
     5521 acgttttgaa gttgtatcta gtcttgcccg taaagtttta gacaaggcag aagatgtcaa
     5581 agagcttgtt gatttagact ttaaccgtgt catctatcta ggcgctggtc ctttctttgg
     5641 acttgctcat gaagctcagc tcaagatttt ggaattaact gctggtcaag ttgcgaccat
     5701 gtatgaaagc ccagttggct tccgtcacgg tccaaaatct cttatcaacg acaatacagt
     5761 tgttttggtc tttggtacaa cgacagacta cactcgtaag tacgacttgg acttggttcg
     5821 tgaagttgct ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc aagcttttgg
     5881 tcttgaaaat gtcaaagaag tggcccttgg ttgtggcggt gtcttgaatg atatttaccg
     5941 tgtcttccct tacatcgttt atgcccaact ctttgcccta ttgacttcac tcaaggtaga
     6001 aaataaacca gatacaccgt ctcctacagg tacagtaaac cgtgtagtac aaggtgtcat
     6061 aattcacgaa tatcaaaagt aagacagtgt ttatgaattc ttgacaagag gatttgtaaa
     6121 ttatcagata aaccatagat tgtcagtacg ctttctatgg tttgtttgct tgagagaaat
     6181 agtaaaagga gaacagaatg aaagcataca cagagcgtgt atttggaaat gttgagggcg
     6241 aggatgtctt ggcctatcga tttgagacag acggtggcta ccaacttgag gttatgactt
     6301 atggtgcgac catcttgcgc tatgtcgcac ctgacaaggc tggaaatttt gccaatgtta
     6361 tcttgggatt tgatgacttt gatagttatg taggcaatag tcccaagcat ggagcaagtg
     6421 taggtcctgt agcgggtcgt attgcaggtg cgacctttga gctcaatggt aagacctatg
     6481 accttgaggt taataatgct agcaactgta atcacagtgg ttcaactggt tgggattcca
     6541 gcttgtttga agttgaagaa gtaagcgatc atggcttgac tctctacaca gagcgtacag
     6601 atgggacagg agggttccct ggaaatctca agatttggat cagttatcac ttggaagaaa
     6661 ctggtgccta tgaaaccatc tacaaggtaa cgaccgatca ggatacgcta gtcaatccaa
     6721 ccaaccacag ctatttcaac ttgtctggtg atttcacgca gacgattgac cgtcatgtct
     6781 tccaactaaa cacagagggc atttactcaa tcgctcctga cggtgttcct gccaaaactc
     6841 cagaagccaa ccgtgatgtg gtcaaacaca tctacaatgg taccttgttg aaggatatct
     6901 ttgcagaaga agatgagcaa atccagctgg catcaggttt ggatcaccca tttgcccttc
     6961 ctgcaggcca tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc ttcctgcttt
     7021 tcaagacaga agctccttgc tttgtggtct acacagcaaa ctttgtggat gaaagtgtca
     7081 tcataggagg tcagccaatg ctacagcaca atgggattgc tcttgaagcg caagctttac
     7141 cagatgccat tcacagtgac cttaaaggcc aagtcattct taaagctggt caaaccttca
     7201 ccagtaagac acgttatgaa cttgttgtga aataaaaaag ctggtcaaaa agtctttaaa
     7261 attaaaataa cgcatattgt caggtgatga aaagtttgat actatgcgtt ttattatgaa
     7321 atgatttact ttatcttatc ctgaaatggt gttatttttg agttatctgg cttgactttc
     7381 ttgatggaaa ttatataata gttgtaataa caataattac aacgagaaaa gaggaccatt
     7441 tatgacctat gaatacaaga gtcacattta tttggcagag acagttttaa atgtaaagga
     7501 tttggccagt caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac
     7561 tgagacagag tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca
     7621 agagagcgga gaagtgaggg aacattatgg tctttaccat ttggccattc tcttgcccac
     7681 acgcaaggct ttagctgatg tcttgaaaca cctgacagat ttacaaattc ctcttgtcgg
     7741 cggtgcagac catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat
     7801 tgaactctat cgagataagc cagtttccac atgggatatt cgagaagatg gacgcattat
     7861 cggagtgaca gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc
     7921 ctttatcctg gcagaaggta cgagaatggg gcatattcat ctttccgtca aggatagtcg
     7981 aaagtccagc cagttttatc aaaaggtgtt agggctagag gataaattca gtgtgcctag
     8041 cgctagttgg attgcggctg gagattacca tcatcattta gcagttaacg aatggggagg
     8101 aaaaggtctg gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt
     8161 cgcacacaaa aaagaactgt taacgattgc ccaacgagca caagaagttg acgcaccaat
     8221 caaatggatg acatcgagcc aattggaaat cacagactca gatggcatcg tgacccgtat
     8281 tcgtttagca aggtaaaatt aggaattttt aataagttat aagtattgct agtttgcttt
     8341 ttgaacggtc tttggtaaca gaactgcgtg agctggatgg gcgagaattt tgacaaagaa
     8401 gctgattaga gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag
     8461 tatattactt gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa
     8521 ggagatcgtg actgtgctga tattgtgacg caactgacag cagtgagatc tagtgtagag
     8581 cgcgtgattg agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat
     8641 gattctgaag ctcaaaagga acgcctagaa aaggctatcc gatacttgat taaacggaaa
     8701 taaatgaatg ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg
     8761 tgagtcaaaa gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtgtt
     8821 gaatgtactg acccccaaaa gttagatttt ttctgtctaa cttttggggt gcagttcata
     8881 agaaccttgg tactatgcgt tttattatgt agagacttat actcttcgaa aatctcttca
     8941 aaccacgtca gcttcacctt gccgtagata tggttactga cttcgtcagt tctatccaca
     9001 acctcaaaac agtgttttga gctgacttcg tcagttccat ccacaacctt aaaacagtgt
     9061 tttgagcagc ttgcggctaa cttcctagtt tgcactttaa ttttcattga gtattacttc
     9121 atcttctcat gaaattgagt ttttgcccag ctcctcttga ttattctact tgaccgggcc
     9181 aagcattcat accaccttct acgttggtaa cggtgaggcc ttgggagctg agaaattggc
     9241 aggtagatgc agagcggact cctccttgac aaatgacata gtattcatgg tcaggtttga
     9301 gttctttgta gccttgctct aaggtactta atggaagatt tttggcacct ggtgcatgtc
     9361 ctgcttggaa ttcatgtgct tcacggacat cgataagttc tagattttca ttttggtatt
     9421 ttccataaaa gtcagccatg ctgatactag tttccatatt ctactccttc tgggttaacc
     9481 attttgtata gtgaataagc gccgtcaagg ttttgggcgg taaatcctgc ttgcttgagg
     9541 ataggctctg cgatatagct gcgcaaacca ctgtggcagc tgacgatgta ggcttgtttt
     9601 ttgtccagtt catcagtttc ctccttgaca ctttgtcagt ttttgataca atagtacaaa
     9661 attagaggag gcaggctatg attcagaaac atgcgattcc cattttagag tttgatgata
     9721 atcctcaggc ggttatcatg cccaatcacg aggggttgga tttgcagttg ccaaagaagt
     9781 gtgtctatgc ttttttaggt gaggagattg accgctatgc gagggaagta ggggcgaact
     9841 gtgttggcga attcgtttct gctaccaaga cctatccagt ctatgttatc aactacaagg
     9901 gtgaggaggt ctgtctggct caggctcctg ttggctccgc tccagcagcc cagtttatgg
     9961 attggttgat tggctatggt gtggagcaga ttatctctac tgggacctgt ggtgtcctag
    10021 ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc tttgcgagat gagggagcca
    10081 gttaccacta tgtggcacct tgtcgttata tggaaatgca gccagaggct attgctgcta
    10141 ttgaggaagt tttggaagac agagggattc cttatgaaga agtcatgacc tggacgacag
    10201 acggttttta ccgagaaacg gctgaaaagg tggcttatcg taaggaagaa ggctgtgctg
    10261 ttgtggagat ggagtgttct gctcttgcgg cagtagctca attgcgtggg gttctctggg
    10321 gtgaattgtt gttcacagca gattctctag cggacttgga ccagtacgac agtcgtgact
    10381 ggggctcgga agcttttaat aaggcgctag aactgagttt agcaagtgtt caccaccttt
    10441 agttgtactg gcaaaggatt tgttttatca taaaatatct agctcatact tttcaaaaat
    10501 atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt tgaggttggg aaaaatcttt
    10561 aaaatcagaa aaacgtatca tatcaggtga tgaaaatttt gatactatgc gttttatgtt
    10621 ggtaagattt agagtgagat gaaatgatac tcttcgaaaa tctcttcaaa ccaggtcagc
    10681 ttcaccttgc cgtatagatg ttactgactt cgtcagttct atctgcaacc tcaaaacagt
    10741 gttttgagca acctgtgact agctttctaa tcgatgcctt ggttttcatt gcctataatc
    10801 aaaaagagaa attttctcct gaaaagcata tagagtagct ggcgttaaaa gctcctgtct
    10861 tgcttttttg acctatagtc atatctatca agtattgttc ttgcctaagc tatcaataaa
    10921 aaggtggcat tttttaggct tggtgttagt gaattttgcc ttatcctatc taagtcattt
    10981 cgagcttttt atggtacaat ggaaacatgt tattcaaatt atctaaggaa aaaatagagc
    11041 taggcttatc tcgtttatcg ccagcccgtc gtattttttt gagttttgcc ttggtcattt
    11101 tactaggctc tcttcttttg agcttgccct ttgtccaagt tgaaagctca cgagcgactt
    11161 attttgatca tcttttcact gctgtctctg cagtctgtgt gacaggtctc tcaacccttc
    11221 cagtagctca cacctataat atctggggcc aaataatctg tttgctcttg attcagatcg
    11281 gtggtctagg gctcatgacc tttattgggg ttttctatat ccagagcaag caaaagctta
    11341 gtcttcgtag ccgtgcaact attcaggata gttttagtta tggagaaact cgatctttga
    11401 gaaagtttgt ctattctatt tttctcacga cctttttggt tgagagcttg ggagctattt
    11461 tgcttagttt tcgccttatt cctcaacttg gctggggacg tggtcttttt agttccattt
    11521 ttctagcgat ctcagccttc tgtaatgccg gttttgataa tttagggagc accagtttat
    11581 ttgcttttca gaccgattta ctggtcaatc tggtgattgc aggcttgatt attacaggcg
    11641 gccttggttt tatggtctgg tttgatttgg ctggtcatgt aggaagaaag aaaaaaggac
    11701 gtctgcactt tcatacgaag cttgtattat tattgactat aggtttgttg ttatttggaa
    11761 cggcaactac tctctttctt gagtggaaca atgctggaac gattggcaat ctccctgttg
    11821 ccgataaggt tttagttagc ttttttcaaa cagtgacgat gcgaacagct ggcttttcta
    11881 cgatagatta tactcaggct catcctgtga ctcttttgat ttatatctta cagatgtttc
    11941 taggtggggc acctggagga acagctgggg gactcaagat tacgacattt tttgtcctct
    12001 tggtctttgc acgaagtgag cttctaggct tgcctcatgc caatgttgcg agacgaacga
    12061 tcgcgccgcg aacggttcaa aaatccttta gtgtctttat tatctttttg atgagcttct
    12121 tgataggatt gattctgcta gggataacag ccaaaggcaa tcctcccttt atccacctca
    12181 tatttgaaac catttcagct cttagtacag ttggtgtaac ggcaaatctg actcctgacc
    12241 ttgggaaatt ggctctcagt gttatcatgc cacttatgtt tatgggacga attggtccct
    12301 tgaccttgtt tgttagcttg gcagattacc atccagaaaa gaaagatatg attcactata
    12361 tgaaagcaga tattagtatt ggttaagaaa ggaaagagca tgtcagatcg tacgattgga
    12421 attttgggct tgggaatttt tgggagcagt gtcctagctg ccctagccaa gcaggatatg
    12481 aatattatcg ctattgatga ccacgcagag cgcatcaatc agtttgagcc agttttggcg
    12541 cgtggagtga ttggtgacat cacagatgaa gaattattga gatcagcagg gattgatacc
    12601 tgcgataccg ttgtagtcgc gacaggtgaa aatctggagt cgagtgtgct tgcggttatg
    12661 cactgtaaga gtttgggggt accgactgtt attgctaagg tcaaaagtca gaccgctaag
    12721 aaagtgctag aaaagattgg agctgactcg gttatctcgc cagagtatga aatggggcag
    12781 tctctagcac agaccattct tttccataat agtgttgatg tctttcagtt ggataaaaat
    12841 gtgtctatcg tggagatgaa aattcctcag tcttgggcag gtcaaagtct gagtaaatta
    12901 gacctccgtg gcaaatacaa tctgaatatt ttgggtttcc gagagcagga aaattcccca
    12961 ttggatgttg aatttggacc agatgacctc ttgaaagcag atacctatat tttggcagtc
    13021 atcaacaacc agtatttgga taccctagta gcattgaatt cgtaaagagg gatgacccct
    13081 cttttttgat gcccaagatg gcaaatagag acagaagccc cttgtcttct agtaaaagtt
    13141 cttcaaagac tggactttat ggtaaaatag aaagaagtga caagagagag taatactcaa
    13201 tgaaaatcaa agatcaaact aggaaactag ctacgggctg ctcaaaacac tgttttgagg
    13261 ttgtggatag aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg
    13321 acgaagtcag taacatctat acggcaaggc gacgttgacg cggtttgaag agattttcga
    13381 agagtataag aaaaaatcag tcccctaaag gagcagatta tgaagttatt gtctatcgcc
    13441 atttctagct ataatgcagc agcctatctt cattactgtg tggagtcgct agtgattggt
    13501 ggtgagcaag ttgggatttt gattatcaat gacgggtctc aggatcagac tcaggaaatc
    13561 gctgagtgtt tagctagcaa gtatcctaat atcgttagag ccatctatca gaaaaataaa
    13621 tgccatggcg gtgcggtcaa tcgtggcttg gcagaggctt ctgggcgcta ttttaaagta
    13681 gttgacagtg atgactgggt ggatcctcgt gcctacttga aaattcttga aaccttgcag
    13741 gaacttgaga gcaagggtca agaggtggat gtctttgtga ccaattttgt ctatgaaaag
    13801 gaagggcagt ctcgtaagaa gagtatgagt tacgattcag tcttgcctgt tcggcagatt
    13861 tttggctggg accaggtcgg aaatttctcc aaaggccagt ataccatgat gcactcgctg
    13921 atttatcgga cagctttgtt gcgtgctagc cagttctaat tgcctgaaca tactttttat
    13981 gtcgataatc tctttgtctt tacgcccctt cagcaggtca agaccatgta ctatctgcct
    14041 gtcgatttct atcgttattt gattgggcgt gaggaccagt ctgtcaatga gcaagtgatg
    14101 attaagtgca ttgaccagca actcaaggtc aatcgactct tggtagacca gcttgatttg
    14161 tcccaagtga gtcatcctaa aatgcgagaa tatctgctga atcatattga aatcacgacg
    14221 gtgatttcca gtaccctgct caaccgatct gaaacagcgg agcatctggc aaaaaaacgc
    14281 caattgtgga cctatattca gcaggaaaat ccaaaagtct ttcaggctat tcgtaagacc
    14341 atgttgagcc gtttgaccaa acattctgtc ttgccagatc gcaaactgtc caatgtcgtc
    14401 tatcaaatca ccaaatctgt ttatggattt aattaatata agtgttttat aagagggatt
    14461 taagaaaaat tttaactttt tcttagtcct ttttaatttc aggagattat actagagtca
    14521 tcaaataaag aaagactcta aggagaatcc tatgaaattc aatccaaatc aaagatatac
    14581 tcgttggtct attcgccgtc tcagtgtcgg tgttgcctca gttgttgtgg ctagtggctt
    14641 ctttgtccta gttggtcagc caagttctgt acgtgccgat gtggtcaatc cgaccccagg
    14701 tcaagtctta cctgaagaga catcgggaac gaaagagggt gacttatcag aaaaaccagg
    14761 agacaccgtt ctcactcaag cgaaacctga gggcgttact ggaaatacga attcacttcc
    14821 gacacctaca gaaagaactg aagtgagcga ggaaacaaac tcttctagtc tggatacact
    14881 ttttgaaaaa gatgaagaag ctcaagaaaa tccagagcta acagatgcct taaaagaaac
    14941 tgtagataca gctgatgtgg atgggacaca agcaagtcca gcagaaacta ctcctgaaca
    15001 agtaaaaggt ggagtgaaag aaaatacaaa agacagcatc gatgttcctg ctgcttatct
    15061 tgaaaaagct gaagggaaag gtcctttcac tgccggtgta aaccaagtaa ttccttatga
    15121 actattcgct ggtgatggta tgttaactcg tctattacta aaagcttcgg ataatgctcc
    15181 ttggtctgac aatggtactg ctaaaaatcc tgctttacct cctcttgaag gattaacaaa
    15241 agggaaatac ttctatgaag tagacttaaa tggcaatact gttggtaaac aaggtcaagc
    15301 tttaattgat caacttcgcg ctaatggtac tcaaacttat aaagctactg ttaaagttta
    15361 cggaaataaa gacggtaaag ctgacttgac taatctagtt gctactaaaa atgtagacat
    15421 caacatcaat ggattagttg ctaaagaaac tgtcgaaaaa gctgttaaag ataacgttaa
    15481 agacagtatc gatgttccag cagcctacct agaaaaggcc aagggtgaag gtccattcac
    15541 agcaggtgtc aaccatgtga ttccatacga actcttcgca ggtgatggca tgttgactcg
    15601 tctcttgctc aaggcatctg acaaggcacc atggtcagat aacggtgagg ctaaaaaccc
    15661 agccctatct ccactaggcg aaaacgtgaa gaccaaaggt caatacttct atcaagtagc
    15721 cttggacgga aatgtagctg gcaaagaaaa acaagcgctc attgaccagt tccgagcaaa
    15781 tggtactcaa acttacagcg ctacagtcaa tgtctatggt aacaaagacg gtaaaccaga
    15841 cttggacaac atcgtagcaa ctaaaaaagt cactattaac ataaacggtt taatttctaa
    15901 agaaacagtt caaaaagccg ttgcagacaa cgttaaagac agtatcgatg ttccagcagc
    15961 ctacctagaa aaagccaagg gtgaaggtcc attcacagca ggtgtcaacc atgtgattcc
    16021 atacgaactc ttcgcaggtg atggtatgtt gactcgtctc ttgctcaagg catctgacaa
    16081 ggcaccatgg tcagataacg gtgaggctaa aaacccagcc ctatctccac taggtgaaaa
    16141 cgtgaagacc aaaggtcaat acttctatca agtagccttg gacggaaatg tagctggcaa
    16201 agaaaaacaa gcgctcattg accagttccg agcaaacggt actcaaactt acagcgctac
    16261 agtcaatgtc tatggtaaca aagacggtaa accagacttg gacaacatcg tagcaactaa
    16321 aaaagtcact attaagataa atgttaaaga aacatcagac acagcaaatg gttcattatc
    16381 accttctaac tctggttctg gcgtgactcc gatgaatcac aatcatgcta caggtactac
    16441 agatagcatg cctgctgaca ccatgacaag ttctaccaac acgatggcag gtgaaaacat
    16501 ggctgcttct gctaacaaga tgtctgatac gatgatgtca gaggataaag ctatgctacc
    16561 aaatactggt gagactcaaa catcaatggc aagtattggt ttccttgggc ttgcgcttgc
    16621 aggtttactc ggtggtctag gtttgaaaaa caaaaaagaa gaaaactaat cagctaagga
    16681 aataaatgat ggatagtggg ctgactaaga ttagtttaac aactcaatca gcaatcagga
    16741 ctttctttca atagcagatt aaaatcatcg taaaacaata aaaatagtgt tatacttaaa
    16801 gcagtatagc actgttttta tcaaaggaga gacagatggg aaagacaatt ttactcgttg
    16861 acgacgaggt agaaatcaca gatattcatc agagatactt aattcaggca ggttatcagg
    16921 tcttggtagc ccatgatgga ctggaagcgc tagagctgtt caagaaaaaa ccgattgatt
    16981 tgattatcac agatgtcatg atgcctcgga tggatggtta tgatttaatc agtgaggttc
    17041 aatacttatc accagagcag cctttcctat ttattactgc taagaccagt gaacaggaca
    17101 agatttacgg cctgagcttg ggagcagatg attttattgc taagcctttt agcccacgtg
    17161 agctggtttt gcgtgtccac aatattttgc gccgccttca tcgtgggggc gaaacagagc
    17221 tgatttccct tggcaatcta aaaatgaatc atagtagtca tgaagttcaa ataggagaag
    17281 aaatgctgga tttaactgtt aaatcatttg aattgctgtg gattttagct agcaatccag
    17341 agcgagtttt ctccaagaca gacctctatg aaaagatctg gaaagaagac tacgtggatg
    17401 acaccaatac cttgaatgtg catatccatg ctcttcgaca ggagctggca aaatatagta
    17461 gtgaccaaac gcccactatt aagacagttt gggggttggg atataagata gagaaaccga
    17521 gaggacaaac atgaaactaa aaagttatat tttggttgga tatattattt caaccctctt
    17581 aaccattttg gttgtttttt gggctgttca aaaaatgctg attgcgaaag gcgagattta
    17641 ctttttgctt gggatgacca tcgttgccag ccttgtcggt gctgggatta gtctctttct
    17701 cctattgcca gtctttacgt cgttgggcaa actcaaggag catgccaagc gggtagcggc
    17761 caaggatttt ccttcaaatt tggaggttca aggtcctgta gaatttcagc aattagggca
    17821 aacttttaat gagatgtccc atgatttgca ggtaagcttt gattccttgg aagaaagcga
    17881 acgagaaaag ggcttgatga ttgcccagtt gtcgcatgat attaagaccc ctatcacttc
    17941 gattcaagcg acggtagaag ggattttgga tgggattatc aaggagtcgg agcaagctca
    18001 ttatctagca accattggac gccagacgga gaggctcaat aaactggttg aggagttgaa
    18061 ttttttgacc ctaaacacag ctagaaatca ggtggaaact accagtaaag acagtatttt
    18121 tctggacaag ctcttaattg agtgcatgag tgaatttcag tttttgattg agcaggagag
    18181 aagagatgtc cacttgcagg taatcccaga gtctgcccgg attgagggag attatgctaa
    18241 gctttctcgt atcttggtga atctggtcga taacgctttt aaatattctg ctccaggaac
    18301 caagctggaa gtggtggcta agctggagaa ggaccagctt tcaatcagtg tgaccgatga
    18361 agggcagggt attgccccag aggatttgga aaatattttc aaacgtcttt atcgtgtcga
    18421 aacttcgcgt aacatgaaga caggtggtca tggattagga cttgcgattg cgcgtgaatt
    18481 ggcccatcaa ttgggtgggg aaatcacagt cagcagccag tacggtctag gaagtacctt
    18541 taccctcgtt ctcaacctct ctggtagtga aaataaagcc taaaacccct ttacaaatcc
    18601 agctattcat ggtagaatag attttgtgtg aaatatcagc aggaaagcat gaagctcgtc
    18661 aacaggtgtc ttatgacaag taaccttggc tgtttaggcg aagggcatct gcacgaatca
    18721 gggctttcta agtgactatt tccaccgaaa tattatttat atcaggagga cattcatatg
    18781 tcacgttata caggaccatc ttggaaacaa gctcgtcgcc ttggcctttc acttacaggt
    18841 acaggtaaag aattggcacg tcgtaactac gtaccaggac aacacggacc aaacaaccgt
    18901 tctaaattgt cagaatacgg tttgcaattg gctgaaaaac aaaaacttcg tttcacttac
    18961 ggtgtaggtg aaaaacaatt ccgtaacttg ttcgtacaag ctacaaaaat caaaggcgga
    19021 atcctaggtt tcaactttat gcttcttttg gaacgtcgtt tggataacgt tgtttaccgt
    19081 cttggtctcg cgactactcg tcgtcaagct cgtcaattcg taaaccacgg tcacatcctt
    19141 gttgacggga aacgcgttga tatcccatca taccgcgtaa ctccaggtca agtgatctca
    19201 gttcgtgaaa aatcattgaa agttccagca atccttgaag cagtagaagc tactcttgga
    19261 cgtccagcat tcgtatcatt cgacgctgaa aagttggaag gttcattgac tcgcttgcca
    19321 gaacgcgacg aaatcaaccc agaaatcaac gaagcacttg tcgttgaatt ctacaacaaa
    19381 atgttgtaat attttattaa ataagatagg ctttgaggcc ttgatattaa gcactttggg
    19441 acgttctctc ttagtgcttt tttgatttct cttagtatcc agctataatc gtttagacat
    19501 aactagaccg atatagtcca aagtgatata gtaaaatgaa ccaaaaatag tacacaatgt
    19561 ggtataat
//
LOCUS       DAVHIU010000026        19139 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVHIU010000026 DAVHIU010000000
VERSION     DAVHIU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.028"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            3..353
                     /locus_tag="VVN06_000811"
     CDS             3..353
                     /locus_tag="VVN06_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5562391.1"
                     /translation="MFSLFEKIKELCQKRGISINSLEETLGYSRNTIYSMKSKKPNAE
                     RLQEIADYFNVSTDYLLGRTDNPVIAGDKVAKAEIDLKKDAAESFFYDGHELNDEDLD
                     LISSLLEARMRNRK"
     gene            363..746
                     /locus_tag="VVN06_000812"
     CDS             363..746
                     /locus_tag="VVN06_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU5562392.1"
                     /translation="MTTPEQVCSEQGIDLVYFDGRGSHNKGLYNQPHNLIAVDTYLDD
                     IEKKKVIYHEIGHKEHDPEQYKRRREEYELQADRNMIHYLLKEELETMDDFTNFNYLH
                     FMEKYNLKTMTNEIMVKEEYLSLVN"
     gene            762..1832
                     /locus_tag="VVN06_000813"
     CDS             762..1832
                     /locus_tag="VVN06_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU5562393.1"
                     /translation="MEIDKVKADLKQVGKRVAELSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEPEEIVEVADEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYADEILEVIKKFS"
     gene            1955..3082
                     /locus_tag="VVN06_000814"
     CDS             1955..3082
                     /locus_tag="VVN06_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5562394.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESG
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(3170..4081)
                     /gene="whiA"
                     /locus_tag="VVN06_000815"
     CDS             complement(3170..4081)
                     /gene="whiA"
                     /locus_tag="VVN06_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU5562395.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(4078..5055)
                     /locus_tag="VVN06_000816"
     CDS             complement(4078..5055)
                     /locus_tag="VVN06_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU5562396.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(5052..5942)
                     /gene="rapZ"
                     /locus_tag="VVN06_000817"
     CDS             complement(5052..5942)
                     /gene="rapZ"
                     /locus_tag="VVN06_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5562397.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(5994..6374)
                     /locus_tag="VVN06_000818"
     CDS             complement(5994..6374)
                     /locus_tag="VVN06_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5562398.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(6385..6972)
                     /locus_tag="VVN06_000819"
     CDS             complement(6385..6972)
                     /locus_tag="VVN06_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU5562399.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(6981..8213)
                     /gene="clpX"
                     /locus_tag="VVN06_000820"
     CDS             complement(6981..8213)
                     /gene="clpX"
                     /locus_tag="VVN06_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5562400.1"
                     /translation="MPTTRNNDMMVYCSFCGKSQEEVQKIIAGNNAFICNECVELAQE
                     IIREELAEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELKFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(8250..8420)
                     /locus_tag="VVN06_000821"
     CDS             complement(8250..8420)
                     /locus_tag="VVN06_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562401.1"
                     /translation="MERSIFGFFTAMLCLVCLLAGAQAFRKKRYGLSVLLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(8420..8926)
                     /locus_tag="VVN06_000822"
     CDS             complement(8420..8926)
                     /locus_tag="VVN06_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU5562402.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWYLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNSEEKIDGVTTFYDVQSVLDWYHAQDKNLYILGG
                     KQVFQAFEPYLDEVIVTQIHARVEGDTYFPEEFDLSLFETVSSKSFTKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(9058..9576)
                     /locus_tag="VVN06_000823"
     CDS             complement(9058..9576)
                     /locus_tag="VVN06_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU5562403.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            complement(10073..11578)
                     /gene="lytC"
                     /locus_tag="VVN06_000824"
     CDS             complement(10073..11578)
                     /gene="lytC"
                     /locus_tag="VVN06_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU5562404.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGVYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(11616..12374)
                     /locus_tag="VVN06_000825"
     CDS             complement(11616..12374)
                     /locus_tag="VVN06_000825"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5562405.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(12473..13150)
                     /locus_tag="VVN06_000826"
     CDS             complement(12473..13150)
                     /locus_tag="VVN06_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU5562406.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(13159..14103)
                     /locus_tag="VVN06_000827"
     CDS             complement(13159..14103)
                     /locus_tag="VVN06_000827"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU5562407.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(14285..14797)
                     /locus_tag="VVN06_000828"
     CDS             complement(14285..14797)
                     /locus_tag="VVN06_000828"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5562408.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(14884..15642)
                     /locus_tag="VVN06_000829"
     CDS             complement(14884..15642)
                     /locus_tag="VVN06_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5562409.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAIDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            15850..15996
                     /locus_tag="VVN06_000830"
     CDS             15850..15996
                     /locus_tag="VVN06_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562410.1"
                     /translation="MEIKEKTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(16143..17273)
                     /gene="ugpC"
                     /locus_tag="VVN06_000831"
     CDS             complement(16143..17273)
                     /gene="ugpC"
                     /locus_tag="VVN06_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5562411.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(17557..17642)
                     /locus_tag="VVN06_000832"
     tRNA            complement(17557..17642)
                     /locus_tag="VVN06_000832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17606..17608),aa:Leu,seq:aag)
     gene            complement(17724..18299)
                     /locus_tag="VVN06_000833"
     CDS             complement(17724..18299)
                     /locus_tag="VVN06_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU5562412.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(18299..19087)
                     /gene="codY"
                     /locus_tag="VVN06_000834"
     CDS             complement(18299..19087)
                     /gene="codY"
                     /locus_tag="VVN06_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU5562413.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         5838 a         4218 c         3390 g         5693 t
ORIGIN      
        1 caatgttttc tttgtttgaa aaaattaaag aactttgcca aaaacgagga atttctataa
       61 attctctcga agaaacactt ggatatagca gaaatacaat ctatagcatg aagagtaaaa
      121 aaccaaatgc tgaaagatta caagaaatcg ccgactactt caatgtctca actgactact
      181 tacttggccg tacagataat ccagttatag ctggagataa ggtcgcaaag gcagaaatag
      241 acctcaaaaa agacgcagca gaaagtttct tttacgatgg acacgaactc aacgacgagg
      301 atttagacct catctcctca ctactagaag ctcgtatgag aaatagaaag taatacttgc
      361 ctatgacaac acccgaacaa gtctgttctg aacaaggtat cgatctagtt tattttgatg
      421 gtagaggttc ccacaataaa ggactttaca accaacccca caatctcata gcggtagaca
      481 cttacctaga tgatattgag aaaaagaaag tcatctatca cgagatagga cacaaagagc
      541 acgacccaga acagtacaaa cgaagacgag aagaatacga actccaagca gatagaaaca
      601 tgatacacta cctgctaaaa gaagaacttg aaacaatgga tgacttcact aatttcaact
      661 accttcattt catggagaaa tacaacctca aaaccatgac aaatgaaatc atggtcaagg
      721 aagaatattt atcattagta aattaaaaaa ggagtatcaa tatggaaatt gataaagtaa
      781 aagctgattt aaaacaagtc ggcaaacgtg tagcagaact cagccaaagc attacgaatg
      841 aagaacaaac aaagaatgcc ttcattatgc cgttcttcca agcgcttgga tatgatatct
      901 tcaatccact tgaatttgtc ccggaattta ctgccgacgt gggcattaaa aaaggcgaaa
      961 aagtcgacta tgcgatcatt ttggatggcg agcctcaaat cctaattgaa tgtaaatcaa
     1021 ttacagaaaa ccttactaag cacgactctc aactattcag atatttcgta actactaaat
     1081 caaaattcgg tatcttgaca aatggtagag aatataaatt ctttactgat ttagacgaac
     1141 cgaataaaat ggatacaact ccattcttga caatagatgt tactgatatc aaagagaacc
     1201 aatttactga gattatcaaa ttccacaaag agaattttga tattgataat attgtttcat
     1261 cagcttctga attgaaatat ctcaacaatc ttaaagcgtt tctgactgaa aatatcacta
     1321 caccttcaga cagcttcctc agatatctaa catcagaaat ctatgaaggt cgtgtgactc
     1381 aaaatatctt aacgacattt tctcctatca tcgtgaaagg atttaatcaa ttcatcacag
     1441 aaagagttaa tgaaaaatta agtgctgcac ttaatacaag cgttgaaaca aaagtaacaa
     1501 ccgatattcc aaaagttgaa gctgaacctg aagagattgt tgaagtagct gacgaaatta
     1561 tcacaactcc tgctgagtta gaagtttata ctgttgttaa aatgcttgct agggatgtag
     1621 tttctccaga acgtgtattt tacagagata accgaagcta tttcaatgtt ttggttgatg
     1681 ataatatcaa aaaatgggta ttacgttatc gttctaattc aaagaaaagc actatcgaaa
     1741 ttcgtgataa aggtatcttc ccagtatcta ctcctctcga agttgcaaat tatgccgacg
     1801 agattttaga agtaatcaag aaattctcgt aacacaaaaa atccccacac tcaaattttg
     1861 gtcgaggaga gtgtgaggaa cttacgtata agaaataacc attcaaaagg tcattttctt
     1921 atacccattt tatcaaaaaa gtgaggtaaa cgcaatgtgg atggaagaac ttccgaacgg
     1981 aaaatacaaa ttttttgagc gatataaaga tccatatact gagaaattaa aaaaagtttc
     2041 agtgaccatg gagaagaaaa ctccccaggc aagaaatcaa gctgctatct tattgcaaga
     2101 gaagataaat aaaaaactaa gcacaaaaca agtagaaagc attacatttg aagaaatcta
     2161 taaccttttt tataaatcat gggcgcaaac agtaaaggaa tcaacaaaac ataattgtaa
     2221 atcagttgat aagaagatga aggaagtcat accatccgat accatacttg ctaatcttga
     2281 caggcgtttt cttcaagagg ctattgaaaa aattattgaa agcaacggat atattacagc
     2341 taaaaaagta cggcataggc tcagaggtat ctttaattac gctgttcaat actcttacat
     2401 tgaaaacaac gaggtcgatt atactacgat tcctcaaaaa ccaaagactt tagaagaact
     2461 ggaaaaaaag cgtaacaact ttctcaccat gcaagaaata aaagcacttg tcgatgtcct
     2521 taatcgtcga gaatatcacc aaaagtacgc tgatatggtt cttgtgctga cattaactgg
     2581 tatgagatat ggtgagttaa ctgccttaca actgaagaat atagacttcg aaaacaacaa
     2641 aattgagatc acaggtaatt ttgattcagt aaacaaaatc aagacgctac caaagactac
     2701 aaattcaata cggacaatca aagtatcaga gagtgtcata gaagctattc aaagacaaat
     2761 agtacgactt agcgaacgtt tccagccatt gtcaagcgat gattatattt tctgttttga
     2821 aaaatggaat caacctacaa caatagcttg cttcatacag atattaaaaa aatatggaaa
     2881 acaggccaaa atagaaaaaa acttatctag ccatattttt aggcattctc atatttcgtt
     2941 tttagcagag tctggcctcc caataaaatc aataatggat cgagttgggc actcaaatgc
     3001 aaaaatgact ttggaaatct attctcatac tactgaagat atggaggata aactggtcaa
     3061 taaattagat actatttttt aattctgccc cttggttgcc cctttttgat ttcataacac
     3121 aacaaaaccc cttgaagata ttgataattc aaggggttgc tttatatcgt tataattcat
     3181 ccgctatctt attgattttt ctgagtctgt gattgacacc acttttggtc agaggggtgc
     3241 tgagactatc tgctaactgc tggatagagt agtctgggtg ctgaatcctc agttgcgcca
     3301 cttcctgcaa atctactggc aaattttcta agcccatgat atctttgatt ttactgatat
     3361 tgttaatggt cttcatgctg gcagaaactg tccgagcgat attagctgtc tcggcattat
     3421 tagcccgatt gaggtcgtta cgggtttctc gcaaaatctt aacccgctca aaatcatcac
     3481 gtgcctgcat ggctccgatt actatcaaga agtccataat gtcttctgct cgctggagat
     3541 aggtcacagc ccccttcttg cgctcaagca ccttggcatc cagtaaaaac tgttggagaa
     3601 gggaggcaat tccttgcgcg tggtccagat aaacagaact gatttccaac tggtacttgc
     3661 ctgactcagg gtcacgaatg ctcccatttg ccaagaaagc gccacagaga taggcacgac
     3721 ctgcttcctc atccgataaa atcgcctcat caatacctgt ttccaggcca aagaaagagt
     3781 ctgccaagtg caaatcactt aacaaatcct gcaccttttc atctgtaaaa acggtataga
     3841 cgcgattctt gcgaagattg ctccgttggt ggtgacgaat ttcagatttg atttcataga
     3901 aatgaagaaa ggactcatag aggtgacggg ccagtttggc attttctgtc acaactgaca
     3961 aagtcaagcc cgaagtcgag agaccgatgc taccagacat tttgataatg gcagataatt
     4021 catgccagct cagatggtgt tggcccagga tttcttcttt tactgctact gtgaaactca
     4081 ttttttcacc tgtataatgc gcatcaactc gtccacaatc aaatctccat cgtggaaggc
     4141 accgccattt tccagacgaa ggaagttaga tgaaatcacg cgcgaaactt gcttacaaag
     4201 acctacaaaa tcgtgttcca cttgcactaa gtattcatca aaacggttgg aattcatgta
     4261 ttcctgaggc actttttcaa tattcaccaa gacagtgtcg ataaaagggc gaccaaggtg
     4321 acgatgcaag acttccacgt ggtcgctatc tgtaaagtgt tccgtctccc cacgttgggt
     4381 catgatattg cagacatagg caatttctgc cttagtttcc aaaagagccc gcccaatttc
     4441 cttaatcacg atattgggca aaatagaggt aaagagggaa cctggcccta ggacaatcat
     4501 gtcactttca aggatggtct gcactactcg acggctggcc agaggcgtat catcgtttag
     4561 ggcattggtc acatagacat tgtcaattat gcctcgatgg tctacaatat gactctctcc
     4621 agccacttct gtcccatcct gaaagactgc atgaagggtc aaaggatggt cactggaagg
     4681 ataaattttc cctgttgtat ggaaaaattt gctcaataac tgcatggcat tataggttga
     4741 accctgcatt tctgacaagc cagcaatgat gagatttccc aatggatggc cagcaaaggc
     4801 tccggcatcc tcagagaacc gatactgaaa gaccttctca taaaacttag gcatatccga
     4861 catggccaca aggacattac gaagatcacc tggcggtgtc aactgttgca tattttttcg
     4921 gagttcacct gaagaaccac catcatctgc caccgtcacg atagctgcga tttccacatc
     4981 tttttcccgc agacttttta gaatgacggg aattccagtc cctccaccaa tcaccgttat
     5041 ctttggtttt ctcatgaacg gtttaccgtt tcctttctgc ggtctttgtc gcgatgccct
     5101 tcattaacag accaattctt ggataagtcc tgcgccaagc gtttagcaaa tgccacacta
     5161 cggtgttgtc cacccgtaca tcccatagca atggtcaaaa cggacttacc ttccttttgg
     5221 taacttggca gaatcggctc aatcaaggcc aataaatgtt gataaaagtc ttctgactca
     5281 ggatggttca tgacataatc ataaacaggt tcatccacac ccgtttggtt tctcagttct
     5341 ggtagataat agggatttgg caagaaacgg acatcaaaga ccaagtccgc atcaatcggg
     5401 attccatact taaatccgaa agacatgact tcgatacgga aagactgggc ttgttcttgg
     5461 tctgaaaact gctctgcaag ggttttgcgc agttcacgtg gagtgagttc agtcgtatcc
     5521 accacatttt ggctcatatt tttcaaaggt gccaagagtt cacgttccaa cttgattcca
     5581 tctaaaatac gaccgtctgc tgctagtggg tgactccgtc tggtttcctt gtaacgagcg
     5641 accaattcct tatcagccgc atccaaaaag aggattttga aatccaaacc atcttgattt
     5701 tccaactcat ccaaaacagc ttgaatctct gaaaagaaag aacggctacg catatccact
     5761 accaaagcca acttaggatt atcttcctta atttcaacca gctgcaaaaa cttaggcaag
     5821 agagctggcg gcatattatc aatggtgaaa taacctagat cctcgaagga ctgaatggct
     5881 acagttttcc ctgcgccact catccctgtc acaatcacca agtgaagttg tttctttgtc
     5941 atctttttct ccttatatca aaagaagttt ggcaacacca aacttcaact agcttatcca
     6001 atctctgcga tgacttcaat ttcgactttt acatcacgag gaagacgagc tacctccaca
     6061 gctgaacgag ctgggaattc ctctttgaag gccgtttggt aaacctcatt aaaaggaaca
     6121 aagtcgttca tatcgctcaa gaagcaagtt gttttgacaa catggtcaaa gtctgttcct
     6181 gcttctgcca aaatagcacc gatgtttttc aagacttgct ctgtctgttc ttggatattc
     6241 tctcctacaa tttccccagt ttcaggggat aggggaactt gaccgctagc aaacaaaagg
     6301 ttgccaacga tttttccttg aacatagggt ccgatagcct ttggggcctt atctgtatga
     6361 attgtttttg ccattttctt ttcctcacaa tttttctaag attgcatccc aagcctcatc
     6421 catccctgcc ttgctgacag atgaaaagag gatgaaatcg tcactcgggt caaagtttaa
     6481 tttctttttg attgctgatt catgcttgtt ccatttacca cgaggaatct tgtccgcctt
     6541 ggtcgccaca atgatgactg gaatctcata atacttgaga aattcgtaca tctgcacatc
     6601 atctgctgac gggtcatgac gaaggtcaac tagactgaca accgcacgga gattttcccg
     6661 agtcgttaag tactcctcaa tcatgcaccc ccacttttca cgttcctttt tagaaacacg
     6721 agcatagcca taaccaggca catccacaaa gcgcatcttg tcatcaatgt taaaaaagtt
     6781 caggagctgg gttttaccag gttttcctga tgtacgggcg agattcttac ggttcaacat
     6841 agtgttgata aagctggatt taccaacatt tgaacgccct gctagggcaa tctctggcag
     6901 ttcatcctgc ggatagtggg acttattagc tgcactgagc aagatttcag cattgtgtgt
     6961 attaagttcc atagtcacct ctaggctgtt tctaggatcg gtttatccgt tccatcgaca
     7021 gtttctttag tgatgcgaac caatttcaca ttttcctgac tcggcacctc aaacataaca
     7081 tctagcatgg tttcttcgat aatggagcga agtccacgcg cccctgtctt ccgttcgatt
     7141 gctttattag caatctcttg aagggcttcg tcgtcaaatt tcaactcaac atcatcataa
     7201 gaaagcaagg tttggtattg tttcaccaag gcatttcttg gctctttcaa gatgcgaacc
     7261 aagtcatcaa cggtcaattg ctcaagagcc gcaaaaacag gcaagcgtcc aatcaactca
     7321 gggataatac caaatttttg aatgtcttca gcgatgattt cttgcatgta tgagctgttt
     7381 tcgtcaatcg ccttattgtt ttgaccaaat ccgatgactt tttcacccag acgttgtttg
     7441 acaatttctt caataccatc aaaagcacca cccacgatga agaggatatt ttttgtatcc
     7501 acttgaatca tctcttgttg tggatgtttg cgtccacctt gaggcggcac gctagcaaca
     7561 gttccctcga taatcttgag aagggcttgt tgcacccctt caccagaaac atcacgtgtg
     7621 atagacacat tctcactctt cttggcaatc ttgtcaattt catccacata gataatgcca
     7681 cgctctgcac gttcgatgtt aaagtcagca acctgcaaga gtttgagaag gatattttcc
     7741 acatcctcac ccacataacc agcctccgtc agagctgtcg catccgcaat agcaaaaggt
     7801 acattcaagc tcttagccaa ggtctgagca aggaaagttt tccctgaacc agttgggcca
     7861 atcatcaaga tgtttgactt ctgcaaatcc acatcttctg actcttcacg cgtatcgtgg
     7921 aaattgatgc gtttgtagtg gttgtaaacc gccactgcta aggcccgttt ggcacgatct
     7981 tgaccgatta catagtggtt caagatatgg agaagttcga ttggttttgg aacttcagac
     8041 aagtctgcca aaacttcctc agccaactcc tcacgaatga tttcctgggc taactccaca
     8101 cattcattac agataaaggc gttgttccct gcgattattt tttgtacttc ttcttggctt
     8161 ttgccacaaa atgagcaata aaccatcata tcattattcc tagttgtagg catgatttcc
     8221 ttccgttcta ttctatactg tcattctatc taaaataagg tcatgtaaaa agcatggata
     8281 ctattgacca aattggtaaa ggcatttaac cagagcagga cagaaagccc ataacgcttt
     8341 ttacgaaaag cctgtgctcc tgcaagtaag cagaccaaac acagcatggc tgtgaaaaaa
     8401 ccaaatatac tacgttccat tagacttcct ttctcttgcg gtattggatg gtaaaatcat
     8461 aagggttttt ctcatctttg gtaaaggatt tgcttgaaac tgtctcaaaa agggacaagt
     8521 caaactcttc agggaaatag gtatctccct ccacccgagc atgaatttga gtcacaatca
     8581 cttcgtcaag gtagggttca aaagcctgaa aaacttgctt cccaccaaga atatagagat
     8641 tcttgtcttg agcatgatac cagtccaaga cagactgaac atcataaaaa gtagtaaccc
     8701 catctatctt ttcttctgaa ttacgtgtca aaatcaaggt ctcccgtttt ggaagcagtc
     8761 gacgccccat cccatcaaag gtcacgcgcc ccatcaagat agcatgattc agagttgttt
     8821 ctttgaagtg ttgcagttct gctggtaaat accaaggcag acgattgtcc ttaccaatca
     8881 caccctcttc atcctgggcc caaatagcta caattttctt agtcatgctt ccatcctttt
     8941 cactgatagt actattttat caaaaaactc aaaaaaagac tggtttggaa tagcttacag
     9001 aatagaaaaa aatctgtaag aaacttctta cagatttata tatattgcta ttatttctta
     9061 caaaccaggt gcttgtccaa gttctgcggc aagcatccaa actgttttct caagttcagc
     9121 cttagcacca acaaaaatat cgtttgtaac atcatcgcct tcttcgtcag tcacatccaa
     9181 cgctttttgg aaaagagtga tgaggtagcg ataaatagct agaacacgtt ccaagctttc
     9241 ttcaacatta cgatattcac cagcttcttc ttcgatttca ctattttgaa ggaactctgt
     9301 caatgtagag aatgggcttc caccgagtgt aatcaagcgt tcgctgattt catccaattg
     9361 accgtcaaga gcttccatgt actcatccat ttttggatgc catacaagga aaccacgacc
     9421 atgcatatac cagtgcactt ggtgcaaagc aacgtgagct acatacaaat cagcaacagc
     9481 ttggttcaag acttcctttg tttttgccaa tgctactggc gctgcttttg tcaatgatgt
     9541 tacgtctttt actgcttctt ttttcaattc taccattttc tttacctcat tcattatttt
     9601 ttgtaaccac ttcttaatga ttactacctt agtatactac taaggaaaac caatagcaag
     9661 acaaagaact tatatgagaa aagttgcaat agactgataa tttaagaatt ctttagaaga
     9721 caaaaaagag gtttggatgc ttatagtcaa tggaaatcca tcagcaaact aggaaactaa
     9781 acccagcttg accaaagcac aagttttgag gtcgcaggta gaactgacca agctgatgtg
     9841 gtttgaagaa attccccaat actataatag attgaaataa gatgaaacca agtcgattag
     9901 gaaaggtaaa tgaatttcta aaatgtttta ccaatcacaa ggtatttgtc tatattcaat
     9961 ctactatata agcttcattt ccgagcaaga tcttgaattt actcgaggat gaactatcat
    10021 aaaacaaaga aaaagagggt gctactatca catccctctt tcaaatcatt gcttaatacc
    10081 aaacgctgac atctacgcgc ccttggattc ctttcatgta ttcagaagag gtatattgcc
    10141 aacctttttc tcctgaataa taagggtttt cccattctaa agcattcgta taggccgcta
    10201 cccagtttac atgttttaaa atatctgggt gttttaaacg cgtctgtaat aaactacgat
    10261 agctatagac atacacattt tgataacccg cctgcttcat cgtgtccatg tacttgttga
    10321 tgattttaac ccaagtatct gtatcacttg gagctctctt gctcttattt acatattccc
    10381 agttctcaac atcataatag atagggtaag acaggttcat attgtatttc tttataagtt
    10441 caatggtctg tttagcgtca ttctcagcat cggtctcatt ttcagcatag gtatagagat
    10501 agacaccata aggaattccc agacggttta actccttaat gttatgcgcc aattccttgt
    10561 cttctttacc gctataacct agacgaacaa tgacaccatc cacctcgttc tcatcaataa
    10621 cctttttcca atcattgata cgaccattgt gctcactaat atcaatgatt ttcttagcat
    10681 gttcggttcc cacttgttct tctctattat taaagaaata gcgtttacca tctctgtgaa
    10741 cccagccgac attcaaatct ttcttttctt tgagctcacc agaggccgca aagatatagc
    10801 gagcaccatc cataatcact tcgtcagtcg ccatagcacc acttccagtc aaatagtagt
    10861 tgccttgcca ctcattccga gccatatagc cccacttctt gaaatagtac catttgccgc
    10921 ccactttttg ccactcttgg ttagcataag ttccatcgga ttttagataa aaataagcag
    10981 aataggctgg atcatagagc cattcatttt gcatcatagc tccttgacca ttcaagaaat
    11041 aacttccttg ccattggctt ttagccatgt aaccccactt cttgaagtag taccacttat
    11101 ttccaatcaa ttgccattct tgatgagcat aaaccccatc tgacttgaga taaaaccaac
    11161 tcttgtaatt actgtcatag atccactcgt tttgggccat atatccacct gatttcaggt
    11221 agtaatttcc atgccattca ttctgcgagt aacgaccatc tgaattaata taaaaccaag
    11281 ccttatagta gttgtcaaaa atccattcac tttgagcttt agaaccatca tcctttacat
    11341 aataatcccc atcccagtgg gcagcagcat tagtctgtac ttctgcgctc gtttgcgttt
    11401 tattagaaga ctgattttgc tctgaactac ttgaagctgt cgttgtatcc tgcgaagttt
    11461 ttgctacttc agtttcattt gcagcgacat gactagttgc caagcctagt aaacagatac
    11521 ttgctaatcc aatttttatt ttcaatcttt cctctcctat aaaaaatgta acagacatct
    11581 gaatgctgtt ccacctagct tttgctactt actgattatt ttacaaagtc aagcaaagcc
    11641 aagaagcttt cagcttcaag tgacgcacca cctacaaggg caccgtcaac gtctgggcaa
    11701 gccatgtatg aagcaacatt ttcaggttta acagaaccac cgtattgaac acgaactttg
    11761 tctgcgactt cttgaccaaa gtcagcagct acaacgtcac gaacaacttt acacattttt
    11821 tgtgcatcgt cttgtgaagc tgatttacca gtaccgatag cccagattgg ctcataagcg
    11881 ataactgagg cagcaacttg ttcagcagtc aatccagcca atgcagcaga tacttgagca
    11941 cctacgaatt cagcagcttt accagcttcg taagtttcaa gtgattcacc acaacagatg
    12001 attggaagca taccgttcgc aaagattgct tttgcttttt tgttgatatc ttcatcagtt
    12061 tcatggaagt agtcacggcg ttctgagtga ccgataacaa cgtagtcagt accgatttct
    12121 ttcaaaactt gtgggctagt ttcaccagtg aaagcacctg cattttcaaa gtagcagttt
    12181 tgagcagcaa ctttaaggtt tgagcctttt gcaacagcaa gaacagttgt caaatcaaga
    12241 gctggagcag cgatacctgc ttcaacaaga tctgatgaag gaagttttga tgcaactgct
    12301 tcaacgaatg ctttagcttc ttctggattt ttgttcattt tccagttacc agcgataaat
    12361 ggtttacgtg acatttcaca tacctctttt ttcaatttat tttctatttt attctatcac
    12421 aattatacac agcttgcaaa tcttactcgg atttcaagcc tgcttgcatg gattaatcct
    12481 tccagagatc catggcattt ctgaaatctg cagatacctg tgtcaactga ggattgcttg
    12541 cttctctttc tgctctcttg gcctcaattt gagccacact cttgattcct tcatggcgcc
    12601 agtttctcaa aatcgcctga atgtacttcc agtttggttt tccattcaaa acagcttcac
    12661 gaagagcctc cttaatcaag tcagcactgg ttccatcttc ctttagtgtc ttggtcaaat
    12721 cctcaatctc aaaaggcgtc aacaatcgtc ccaactcctg ctggaaggtt tccaccaaat
    12781 ccttgagctg gttttgaggt gtcagctggt ctgaacttga atgaactgct ccaaacaggt
    12841 catccaaacg ttccaaggcc aaactagcat caaagagcaa ttcaatttcg ccatttaatt
    12901 cgatagtacg atactggagc agtccccttt ccgtcagatt agaaatggac tggttgacat
    12961 ccgaaatttc cttgccaatc ctttcagcaa tctggcttgg cgacatttct tctaagcctg
    13021 tcgtattttg caaatagaaa aattgccaaa ccagaaaatc gtcgctagaa ggaaagagtt
    13081 ccttaaaatg caagagcagg gcactcggta aaaccaagat acctgattta aaagcgtcta
    13141 aatatgtcat aattcctctt ataaataccc atatgcagat gcatcatctt ctatctttct
    13201 ccaatcaaaa ggcgtctcct gatagaccgc atggtttatc caattactga aaaagagggc
    13261 tgcagatgaa gaccaacaaa gacaaggtac ctgattaaca tcatcatctt taaagtaatt
    13321 ttctggaata tgtgggtcga aacctgcatc acgatctcga aaatactctt ttgccaaggt
    13381 atcacggtca tactccaaat gaccaaaact ataaatttct cgtaaatcac gactggccaa
    13441 aatagaaacc ccaacctgag gtccttctga taaaatctcg agattggtct tgtttaagac
    13501 ctcttcctta gaaatctccg tgtgccgtga atgaggggat acatagctat catcaaagcc
    13561 tctaaataga aggtgaccct cttttagggt gtcctgagga taaatacctg atagcttact
    13621 gtccatctgg tatttttcta caccatagcg cagataaagc ccagcctgag ccccccaaca
    13681 gatatgaagg gtcgaataga catgagtctt agaccactca agcatctgtc taaattcctc
    13741 ccaatagtcc acttcctcaa atggtaaatg ctcaactgga gcacccgtga tgatcatccc
    13801 atcaaaatac tcatccttga cttcaggaaa agttttatag aaggtctcca tgtgctctga
    13861 acgagttgtt ttagaacggt ggctctccat atagagaaaa tcaatatcca gttgtagggg
    13921 tgtattagcc aagtggcgca acaactgggt ctctgtgacc attttctgtg gcatgagatt
    13981 taaaattaaa atcttcaaag gacggatatc ttggtgggca gcacgttgat catccatgac
    14041 aaagatattc tctgtccgta aaatctcaac agctggcaat tttttatcaa ttcgaatcgg
    14101 cataaccctc tcctaactgt actctttcag gaataattgc atatgtttga agacaaatct
    14161 caaacactta gtccttttat tatactgtaa gaagatatag ttttcaatta tagtttttct
    14221 ctaactagtt atagcctatt tttatatcct agtgtagaga aaacagccct agggactgtt
    14281 ttcattaata atgcataaga actttgtagt cgtagtcacc aattttttca cggccgttca
    14341 attcatccaa ttcaacaagg aaggcacaac ctgccataac accaccaagt ttttcaatca
    14401 tctcgatagt tgccttaaca gttccacctg tcgccaaaag gtcatctaca ataagaacac
    14461 gttgacctgg cttaatggca tccgcgtgca tagtcaaggt atcgacaccg tactcttttt
    14521 catagtcagc agaaataact tcgcgtggca atttacctgg cttacgaaca ggcgcaaaac
    14581 caattcccaa ctcaaaggca actggacaac ccacgataaa tccacgagct tcaggtccca
    14641 cgatcatgtc gactttcttg tcagtagcat actgaacgat ttcacgaaca gcgtagctat
    14701 aagcatttcc atcagccatc aaaggactaa tatcacggaa ggtaatgcct tcctttggat
    14761 aattttcaat tgttgcaatg taatctttta aattcatctt tttctttctt tcaaagtttt
    14821 ttactctcta ttatagcata ttttttaaga aagaaaaaag gaaaagttaa cttcaataat
    14881 tatctaacgt tttgacgatt tataactagc catcgcaata aagcccaatt tctgtttatt
    14941 cttagcaaac attttataca tagttaaaaa ctgctttcta ttctcctttt tacaagcatt
    15001 tacacaaatt ttcaaagttc ctagcaaacc ttcgtcataa atcatacccg ataatttcat
    15061 taatgtcatt tcaccagtca atgctttcac atcacaataa cctgattcta tcatcacctg
    15121 ttcccaacca tcttgagtta aaggacctac atttacatga attgcttgtg ataattcctg
    15181 tctgatagac tctttagctt ccttaagaag cacatcatgt gtcaagagaa gacctccagg
    15241 ttttaatacc cttagatatt ccattacaca ttttttctta gcttgatcgg cttgcatagt
    15301 cagcatagct tcatttataa caatatcaaa actagcatct tgataaggaa gtttcattgc
    15361 atttgctctt tcaaaactga ttaaatgagc aacacctgcc gttccagcag attttttagc
    15421 cacttctaaa gcttgagcat ccatatcaat agcagttatc ttgcaaccaa aacgctgtgc
    15481 caactcaatt gctgtagttc ccctattaca cgcaacctct agtattctct tttcttttga
    15541 aaatcctcct tctgcaatta accaatctgt ggcacgcttt ccacctggac gtaagcgttt
    15601 tttccccaat tttgctaaaa acttatgacc tgcttctgac atttgaacac ctctgttttt
    15661 tcttcattat aacacaaaat gatattatct gacaattcta atctattatt tgataaacta
    15721 gaatcaaaag catacaagtc accataacta tagcataaat ttattattac ctctctagtt
    15781 cgaattcaat tgctcacgta gtttacgcat atagcttcac ttgtagatgg tacccacaaa
    15841 taatactcaa tggaaatcaa agagaaaact agaaaactag ccgtaagctg ctcaaagtac
    15901 agctttgagg ttgcagataa aactgacgaa gtcagtaatc atacgtacgg caaggcgacg
    15961 ttgacgtggt ttgaagagat tttcgaagag tattaaagag catcacaata tagatgcata
    16021 atatgacttc cctagcaaaa aggagagaag cattttgttt ctctccctag attaactgat
    16081 atctctccac tacaattgat aaagagatat cttttcttgt agtgctttga attttattta
    16141 ttttagtaga ttgttttttc agtttctaca tcgaagaagt gtgctttgtt caagtcaaat
    16201 ccaagctcaa ctgttgcacc tgtttgcaag tagtcacgag catcaacttt tgcaacaaac
    16261 tcgtctttac caacttggca gtaaaggtga gattctgaac caagcagttc tgatacagag
    16321 atagtcgctt ttacaacaca gtctgggaat gtttcaagga aagcaggttc tgcattcacg
    16381 tcttctggac ggataccaaa gatcaattct tttccttcgt agcctttttc acgaagaact
    16441 ttcaatgctc cttctggcac tttcaaacgg aaaccgtcag aaacaatttc gctaccaacc
    16501 aatttcacgt tgatgaagtt catagctggg cttccgatga atcctgcaac gaatttgtta
    16561 actggatttt tgtaaacttc ttgaggagta ccgatttgtt ctacacgtcc gatagtacct
    16621 gtaccagcag ggttcttagt agctgacata ataacgatac ggtctgcaag tgtcatcgct
    16681 tctgtttggt cgtgagttac atagatagtt gtagctccga tacgacggtg aattttagcg
    16741 atttcagcac gcattgatac acgaagtttg gcatccaagt ttgacaaagg ttcgtccatc
    16801 aagaatactt tcgcatcacg gacaatcgca cgccccatgg caacacgttg acgttgacca
    16861 cctgaaaggt cagctggttt acgttccaag aattctttca atccaagtat ttcagctgct
    16921 tcttgaacac gtttgttaat gtcttctttg ctgtatttac gcaatttcaa accgaaagcc
    16981 atgttgtcat aaacagtcat gtgtgggtaa agagcgtagt tttggaatac catggcgata
    17041 tcacggtctt ttggagctac gtcgttgaca accacgccat cgatagatgc agtaccttct
    17101 gtaatgtctt caagaccagc aatcatacgg agtgtagttg atttaccaca tcctgaaggt
    17161 cctacgaaaa cgataaattc tttatctttg atgttcaagt tgaaatcttc aactgaatag
    17221 tgttcgctgt ttggatattt tttgtaaata tttttaagat tcaattctac catgaggtga
    17281 actccttttg tcttttgata attatattat agatgaaaac gctttatatt tctatggcaa
    17341 gatgaccaaa aaaataaaaa agttctgtgc aacttgcaca aaactttaga gaatatcgga
    17401 gaatttgaag aagataatct ttattattct tctatagaca aaagcagaaa caaggatcag
    17461 ttttcccata ttttcgctga ttctccacta catttgactg gattctaatt ttttagagaa
    17521 atacaaaaga gctagcttta gctagctctt ttcctatgcg gagagaggga cttgaaccct
    17581 cacgacctaa agcggtcaca ggatccttag tcctgcgcgt ctgccaattc cgccatcccc
    17641 gcgtcgatta ctttactagt atatcaactt ttgggatgct tgtcaacact ttttttcaaa
    17701 ttttttcatt ttcaccaacc aggttactca gaaagttcat ttagattttc atctactaac
    17761 ttagctccga gtgtattttt gaaatgacct agggcaaatt gatgattttc aggccagatg
    17821 gaagcaacag ctggtttaac aatctcgatg tcatatccta gattataggc atctatagct
    17881 gtatgtagga cacagatatc cgtcaagaca cctgttaaga taacggtaga cactctacgc
    17941 tctctcaaac gaatatctag gtcagtccct gaaaaagctg agtaatggcg tttatccatc
    18001 caaaagacac gactgtctga accatgctct tgataaaaga tccccaaatc tccatataaa
    18061 ttccgtccac tcgtcccaat cagattatga ggaggaaata acttactttc cggatggaaa
    18121 caatcgtttt cttcatgagc atcaatagta aagaagatat aatctcctcg ttcaaaagct
    18181 aatcgagtta ccttgctgat ggcatccgaa atcgcctgag ctggagcacc tgctgttagt
    18241 ttcccactat cagcaacaaa atcttctgta taatcaatcg aaattaaagc ctttgtcatt
    18301 agtaatctct tttcttcact tcttcaaaaa tatctgaaat caagacctta agataggttc
    18361 ccttcattcc aagtgagcga ctttcaataa tccccgcaga ctcaagttta cgaagagcat
    18421 tgacaatcac agagcgagtg attccgatac gatctgcaat cactgacgca gtcaacttcc
    18481 cttcatttcc atttaattcc cctaaaattg ctgaaacagc acggagttcg gagtaagaaa
    18541 gggtattgac cgccatggtg acagcagtac gacgacgaat atttttctca tcttcttcac
    18601 gttggaagtt aagaagctga atcccaacaa cggtactggc aatctcaaca agaaccaagt
    18661 cctcatcttc gaatttttta tcattacgcc aaataatcaa agaaccaagg cgaatccccg
    18721 atacatgaat cggtgcaata gtcgtcaagc catctggaaa atcatctcta ctctcaacag
    18781 ggaaaatact catatcatgc tcaacaggta agtttgcttc tgtttcgtaa atcatattag
    18841 ccccttgaac gtagtcatct gggaaaatct tagtttggaa gaattgctct acgcgatctg
    18901 tatttgtttt ataacgcata aaatagccaa gcagacgtcc cttactattg ataatgcagg
    18961 cattgcaatg aataatatcc gctaactgac gcgtaatagc gttgtaagga agctcatcct
    19021 gcaactgctc ctctgagcgt ttcaaaattg atgtaatttt tctagttttt tctaataaat
    19081 gtgccatttt tcacctcgaa tttaatcgct atcattataa cataaaaacg tctcttttt
//
LOCUS       DAVHIU010000027        18842 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVHIU010000027 DAVHIU010000000
VERSION     DAVHIU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.029"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            843..2102
                     /locus_tag="VVN06_000835"
     CDS             843..2102
                     /locus_tag="VVN06_000835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5562414.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2118..2669
                     /locus_tag="VVN06_000836"
     CDS             2118..2669
                     /locus_tag="VVN06_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562415.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2662..3939
                     /locus_tag="VVN06_000837"
     CDS             2662..3939
                     /locus_tag="VVN06_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5562416.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3955..4815
                     /locus_tag="VVN06_000838"
     CDS             3955..4815
                     /locus_tag="VVN06_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU5562417.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5196..6000
                     /locus_tag="VVN06_000839"
     CDS             join(5196..5564,5566..6000)
                     /locus_tag="VVN06_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU5562418.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMSTLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     SHDMKLFKMSCRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            6192..8483
                     /gene="pcrA"
                     /locus_tag="VVN06_000840"
     CDS             6192..8483
                     /gene="pcrA"
                     /locus_tag="VVN06_000840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU5562419.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8526..9206
                     /gene="radC"
                     /locus_tag="VVN06_000841"
     CDS             8526..9206
                     /gene="radC"
                     /locus_tag="VVN06_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5562420.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9203..9892)
                     /locus_tag="VVN06_000842"
     CDS             complement(9203..9892)
                     /locus_tag="VVN06_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5562421.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(9910..10551)
                     /locus_tag="VVN06_000843"
     CDS             complement(9910..10551)
                     /locus_tag="VVN06_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5562422.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10634..10849)
                     /locus_tag="VVN06_000844"
     CDS             complement(10634..10849)
                     /locus_tag="VVN06_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU5562423.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(10857..11204)
                     /locus_tag="VVN06_000845"
     CDS             complement(10857..11204)
                     /locus_tag="VVN06_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU5562424.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11209..12324)
                     /locus_tag="VVN06_000846"
     CDS             complement(11209..12324)
                     /locus_tag="VVN06_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5562425.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12334..13293)
                     /locus_tag="VVN06_000847"
     CDS             complement(12334..13293)
                     /locus_tag="VVN06_000847"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5562426.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13406..13975)
                     /locus_tag="VVN06_000848"
     CDS             complement(13406..13975)
                     /locus_tag="VVN06_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU5562427.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            14103..14774
                     /locus_tag="VVN06_000849"
     CDS             14103..14774
                     /locus_tag="VVN06_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU5562428.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            14758..15576
                     /locus_tag="VVN06_000850"
     CDS             14758..15576
                     /locus_tag="VVN06_000850"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU5562429.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            15573..16469
                     /locus_tag="VVN06_000851"
     CDS             15573..16469
                     /locus_tag="VVN06_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5562430.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     DIRLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLDDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            16513..17487
                     /gene="pta"
                     /locus_tag="VVN06_000852"
     CDS             16513..17487
                     /gene="pta"
                     /locus_tag="VVN06_000852"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU5562431.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(17802..18842)
                     /locus_tag="VVN06_000853"
                     /pseudo
     CDS             complement(17802..18842)
                     /locus_tag="VVN06_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5592 a         3434 c         4133 g         5683 t
ORIGIN      
        1 gtctggggat agaccgtttt aagtctgacg ctggaaataa gaattgtcag aagaagggat
       61 agcgaaatcg tggctctacg aacaggaacg tgataataag gcgtatatag cggataaggg
      121 ggcctcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgcgtaaa tcacgagagt
      181 aattgaattc gtactaagat tttctatttt cactgtaacc ttttaacgtc cttatatctt
      241 gtatacacga ggaaagatgt acgacttatc ccgtgaggtc tatcactata aagagaaaac
      301 gacagataga agcgatcctg agtcacggtt atctttaaaa tagattgtct gataggatgc
      361 tagcctgaaa acgtcttgtt catgaaatat agggacggta tgtataaaac gcttctgtga
      421 actgagagaa gggggagaag ttcttgataa aatttagttg aacagccgta ttccgatact
      481 tagataagag atctagtctt agctcctact cggtttcaga ggataaaaaa gggcaatagc
      541 gattcgagaa agattatact cttcgaaaat ctcttcaaat cacgtcaata tcgccttgcc
      601 gtacgtgtag gatactgact acgtcagttc catctacaac ctcaaaacag tgttttgagc
      661 aacctgcggc tagtttccta gtttgatctt tgatttttat tgagtattag taattcagtt
      721 actaactcgt caactctgat ttatccaata aaattgaaaa ggatggaaaa aaggataaat
      781 ttatgatata ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta
      841 gaatgagaaa aattgttatc aatggtggat taccactgca aggtgaaatt actattagtg
      901 gtgctaaaaa tagtgttgtg gccttaattc cagctattat attggctgat gatgtggtga
      961 ctttggattg tgttccagat atttcggatg tagccagtct tgtcgaaatc atggaattga
     1021 tgggagctac tgttaagcgt tatgacgatg tcttggagat tgatccaaga ggtgttcaaa
     1081 atattccaat gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga
     1141 gcctcttagg ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc
     1201 ctcgtccgat tgacttacac cttaaggcgt ttgaagctat gggtgccact gctagctacg
     1261 agggagataa catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca
     1321 tggatacggt tagtgtggga gcaacgatta atacgatgat tgctgcagtt aaagcaaatg
     1381 gtcgtactat tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct
     1441 tgaataatat gggtgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg
     1501 ttgaaagatt acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat
     1561 atatatcttt agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac
     1621 acctggaagg gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag
     1681 acagcatttt tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt
     1741 acccaggctt tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg
     1801 gtcgtggtac aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag
     1861 caaagatgga tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg
     1921 atttacgtgg ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg
     1981 ctgggcttat ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt
     2041 attctgatat tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt
     2101 aaaccgtaga ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa
     2161 cggatcgctt gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag
     2221 cttcaaatcc agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc
     2281 aatatgcact ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtgacc
     2341 agaaaaatca acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag
     2401 aagctgagct tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg
     2461 ttgttagaaa aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca
     2521 ttacccacct tgaaaataaa gctagccaaa gagttgctct taagtctgga tttagtttgt
     2581 tccgtcagtt taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc
     2641 ggtatgtaaa aggagagttc aatgagtaag catcaggaaa ttctaagcta tttggaggaa
     2701 ttaccagtag gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat
     2761 ggaacagcct atcgggctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct
     2821 agaagtggaa caattcgtgt taaatcccag aaagttgcta tagagagatt aacgtttgct
     2881 gaaattgcag aagtgacttc ttctgaggtt ctggctgggc aagaaggttt agagagagaa
     2941 tttagtaagt tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat
     3001 ggggggctct tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa
     3061 aatgcagttc tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat
     3121 caaaaaggga ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccatgatc
     3181 aataaagcct tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat
     3241 cgccctagtc atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac
     3301 ttggttcgta agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt
     3361 ggcgttgtaa ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt
     3421 atgtctcgta gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg
     3481 atgatcgcag aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc
     3541 gttgtgacgc gacgagatgt catggagaag atgagccgtt cccaagtttc ggctctacca
     3601 actttttctg agcagattgg acaaaagctc tcttatcacc atgatgaagt agtcattaca
     3661 gtggaaccct ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt
     3721 ctgacccaca tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag
     3781 atgctaatct actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg
     3841 attattcatc atacgagacg gtcagctata attgattacg atatttatca tggtcaccag
     3901 attgtttcaa aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata
     3961 acattaaaat cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt
     4021 attcatatag gcttacgtga tttgattaag ccaggcgtag atatgtggga agttgaagaa
     4081 tatgtccgcc gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt
     4141 gccatgatgg actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct
     4201 ttccctcgtc attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga
     4261 ggtcccattg ctaaatctga cctaaatgtc tcaaaattaa acttcaacaa tgttgaacaa
     4321 atgaaaaaat acactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct
     4381 gttggtacac cgtccgaaga agtcaaaaac ttgatggatg taaccaaaga agctatgtac
     4441 aagggtattg agcaagctgt tgttggaaat cgtatcggtg atatcggtgc ggctattcaa
     4501 gaatacgctg aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc
     4561 ccaactatgc acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt
     4621 cttcgtgaag gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt
     4681 gatacagata tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag
     4741 tatgaacacc aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa
     4801 gaaggaactt attaataaaa agtgaaaaga ctactggaag tttattttga taaaaaatcc
     4861 agtagatctt ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt
     4921 tttctgcttt taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag
     4981 gattcagggc tctcctccta tatactatta gtaaagtaaa actaagagag gatattttag
     5041 tgtcgcagtc tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga
     5101 gaaatggaat tctgtttaac gttcgtattg ccaatcttaa atttaccttt tttagattag
     5161 acttcctgcg aaataaaata tggtatagta gttctatgaa tgatgaagca agtaaacaac
     5221 taactgatgc acgatttaag cgtcttgttg gtgttcagcg cacgactttt gaagagatat
     5281 tagctgtatt aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat
     5341 taagcctaga agaccttctt atgtccactc ttcaatatgt gcgagaatat cgaacttatg
     5401 aacaaattgc ggctgatttt ggtattcacg aaagcaactt aatccgtcgg agccaatggg
     5461 ttgaagtaac tcttgttcaa agtggtgtta cgatttcaag aactcctctc agttctgagg
     5521 acacggtaat gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa agaattagcg
     5581 aatcattctg gtaaaaagaa atttcacgct atgaaggctc aagcgattgt cacaagtcaa
     5641 gggagaattg tttctttgga tatcgctgtg aactatagtc atgatatgaa gttgttcaaa
     5701 atgagttgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg
     5761 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta
     5821 acagctgaag ataaagcctg taaccatgcg ctatccaagg agagaagcaa ggttgagaac
     5881 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
     5941 cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact aggattctag
     6001 ttttgcagga agtctattga ggtattgagc tagtttatga aaaaattggg tgaaaaagtc
     6061 gagtgtttta gaaacccaca gtgtagtatt ctagtttcaa tccactatat tttgctactc
     6121 cccgtaaagt ttctattttc cctgatttct gatataatag aaatattgac ttcaagagta
     6181 aggaagagaa gatgaacgca ttattaaatg gaatgaatga ccgtcaggct gaggcggtgc
     6241 aaacgacaga aggtcccttg ctaatcatgg caggggctgg ttctggaaag actcgtgttt
     6301 tgacccaccg tatcgcttat ttgattgatg aaaagctggt caatccttgg aatatcttgg
     6361 ccattacctt taccaacaag gctgcgcgtg agatgaaaga gcgtgcttat agcctcaatc
     6421 cagcgactca ggactgtctg attgcgacct tccactccat gtgtgtgcgt attttgcgtc
     6481 gcgatgcgga ccatattggc tacaatcgta attttacaat tgtggatcct ggtgaacagc
     6541 gaacgctcat gaaacgtatt ctcaaacagt tgaacttgga tcctaaaaaa tggaatgaac
     6601 gaactatttt ggggaccatt tccaatgcta agaatgattt gattgatgat gttgcttatg
     6661 ctgcccaagc tggcgatatg tatacgcaaa ttgtggccca gtgttataca gcctatcaaa
     6721 aagaacttcg tcagtctgaa tccgttgact ttgatgattt gattatgctg accttgcgtc
     6781 tctttgatca aaatcctgat gttttgacct actaccagca aaaattccaa tacatccacg
     6841 ttgatgagta ccaagatacc aaccacgctc agtaccaatt ggtcaaactc ttggcttccc
     6901 gttttaaaaa tatctgtgtg gttggggatg cggaccagtc tatctacggt tggcgtggtg
     6961 ctgatatgca gaatatcttg gactttgaaa aggattaccc caaagccaag gttgttttgt
     7021 tggaggaaaa ttaccgctca accaaaacca ttctccaagc ggccaacgag gttattaaaa
     7081 ataataaaaa tcgccgtcct aaaaatctct ggactcaaaa cgctgatggg gagcaaatcg
     7141 tttactatcg tgccgatgat gagctggatg aggctgtatt tgtagccaga accatcgatg
     7201 aacttagtcg cagtcaaaac ttccttcata aggattttgc agttctctat cggactaatg
     7261 cccagtcccg tacaattgag gaagccctgc tcaagtctaa cattccttat accatggttg
     7321 gcggaaccaa attctacagc cgtaaggaaa ttcgcgatat tattgcttat ctcaacctta
     7381 ttgctaattt gagtgacaat attagttttg agcgtattat caacgagcct aaacgtggaa
     7441 ttggtctagg tacagttgag aaaatccgtg attttgcaaa tttgcaaaat atgtctatgc
     7501 tggatgcttc tgctaatatt atgttgtctg gtatcaaggg taaggcagcc caatctatct
     7561 gggattttgc caatatgatg cttgatttgc gggagcagct agaccactta agcattacag
     7621 agttggttga gtccgtccta gaaaaaacag gttatgtcga tattcttaac gcccaagcga
     7681 ctctagaaag caaggcacgg gttgaaaata tcgaagagtt tctttctgtt acgaagaact
     7741 ttgatgacac cacggatgtg acagaagagg aaactggtct ggacaaactg agtcgtttct
     7801 taaatgactt ggctttgatt gccgacacag attcaggtag tcaggagaca tcagaagtga
     7861 ccttgatgac cctgcatgct gccaaaggtc tcgaatttcc agttgtcttt ttgattggga
     7921 tggaagaaaa tgtctttcca cttagtcgtg cgactgaaga tccagatgaa ttagaagaag
     7981 agcgccgtct agcctatgta ggtatcacgc gtgcagagaa aattctctat ctgaccaatg
     8041 ccaactcacg cttgcttttt ggtcgtacca attataaccg tccgactcgt tttattaacg
     8101 aaatcagttc agacttgctt gagtatcaag gtctggctcg tcctgcaaat acaagcttta
     8161 aggcatcata tagcagtggt agtatttcct ttggtcaagg tatgagtttg gctcaggctc
     8221 ttcaagaccg taaacgcggt gctgccccaa aatcaatcca gtcaagcggt cttccatttg
     8281 gtcaatttac agctggcgca aaatcagcat ctagcgaggc aaattggtcc attggtgaca
     8341 ttgctctcca caagaaatgg ggagagggaa ctgttctgga agtttcaggt agcggtgcta
     8401 ggcaggaatt gaaaatcaat ttcccagaag taggtttgaa aaaactttta gccagtgtgg
     8461 ctccaattga gaaaaaaatc taattttcca tccttctcac gaataataaa gtgaggagga
     8521 tttttatgta cagtatttca ttccaagaag attcactatt accaagagaa aggctggcca
     8581 aggaaggagt tgaagcgctt agtaaccaag agttgctagc tattttactc aggacaggaa
     8641 cacgtcaagc tagcgttttt gaaattgccc aaaaagtctt gaacaatctt tcaagcctaa
     8701 cggatttgaa aaaaatgacc ctgcaggaat tgcagagttt gtctggtatt gggcgtgtta
     8761 aggccataga attacaagct atgattgaac tggggcatcg tattcacaaa cacgagactc
     8821 ttgaaatgga aagtattctc agcagtcaaa agttggccaa gaagatgcag caggaattag
     8881 gggataaaaa acaagagcac ctggtggcac tctatctcaa tactcaaaat caaatcatcc
     8941 atcagcagac catttttatc ggttctgtaa ctcgtagtat cgctgaaccg cgagagattc
     9001 tgcactatgc aatcaagcat atggcgactt ctcttatctt ggtccacaat catccttcag
     9061 gagcggtagc gcctagccaa aatgatgatc atgtcactaa acttgttaaa gaagcctgcg
     9121 aactgatggg gattgttctc ttggaccatt tgattgtctc tcattctaat tactttagtt
     9181 atcgtgaaaa gacagattta atctaaagtt cattaacgac atagtcaaag agtttcttat
     9241 ctttgggacg attttcaaaa agaagttctg gatgccattg gacaccgaga aaggcgacat
     9301 catccgtact catgacagcc tcaatgatac catctttagg atcatgagcc acaactttta
     9361 aatttggtgc taagtccttg atgctctggt ggtggaagga gttgatatga gagatttctc
     9421 catagatttc ttggagaacg gtatctggtt ctgttaccaa gcgttgagtt gtgtactcaa
     9481 cagaagaatc ctgccaatgg tcttcgatat cttggtacaa agttccaccc atggcaacgt
     9541 taaagagttg ggtaccacgg cagacagaga aaatgggctt tttctgttta atagcttcct
     9601 tgatgagggc cagttcgaag atatctcttt gaaggtgata atcatcgcta tcaatggttt
     9661 tgggttcgcc atagaatttt ggatcgacat tttgcccacc tgtcaagatg agcttgtcaa
     9721 tcaaactgat atagtggcag gccatttctt gatcaccaat cggtaggatg atgggaatcc
     9781 ctccagcatc tttaacgcct tcaacaaagc cttttgctgc gtagctcatc atgatgtcat
     9841 catctggatg agttttttcg tttcctgtaa tcccaataac tggttttttc ataaaatgat
     9901 tttcgctttc taatcctctt ttcgcatgaa gtagaggagg gtttggagtt cacttgtcaa
     9961 atcgacatac tgaacgacca cgtcttttgg taaatgcaga tggactggtg aaaaactgag
    10021 aattcctttc acaccagcat caaccaagag attagcaacc tcttgtgact tgacgctggg
    10081 aacagttagg atagcagtct tcacatcagc atccttgatt ttatccttga tctgagaaat
    10141 cccgtaaatg ggaatcccgt caggagtttg ggtaccgact tcaggatggt cgtctaggtc
    10201 aaaggccatg ataatcttca tcttgttacg ttcgtggaag cggtagtgga gaagggcatg
    10261 gcccatattt ccaataccaa ccagcatgac attggtaata gagttgtcat tgagcaaatc
    10321 ggcaaaaaat gtcattagtt ttttgacatc atagccaaaa ccacgacgac caagttcacc
    10381 aaaataggaa aaatcacgac gtacggtcgc tgaatcaata ccgatagcct ctgcaatttg
    10441 cttagagttg gcacgttcaa tcttttctgc atgaaatctc ttaaaaattc gatagtagag
    10501 agagagtctt tttgctgtag cttttggaat agcaaactgt ttatctttca caaaatcaca
    10561 acctttctat tcttctattt tatagaaaca ttgtgaaaaa atcaacaaaa ataagaaaaa
    10621 actaagaaaa atcttagttt tgatgtaaaa aatctgcatg agatagaaaa cggtagaggt
    10681 ctccgaccag cccctgataa acttttttgc ccctaaaagt cagagaagtc acataaagtg
    10741 tatctggtaa ggttacacat cctgacaaag tcaacatgag agcctcatga tcctcatact
    10801 tgagagtacg ctctacatga tagcagtcct tataggtcag ttcaaacatt ttggctctat
    10861 ctttccgatt ttgtaaagac accacgttct accaagctat ccatgaggaa gtagaatttt
    10921 tcctgatgaa tatggtggtc ttctgatttg aaaatatcaa ctagacgaag gccaaacttg
    10981 tcagtgatat tgattttagc ccctgtaagt tccttgttaa tgatgatttt gagttggaag
    11041 ccttcaccgc tgtttggcac tttttccaaa aggcgagtca gttcatagtt accaacctta
    11101 gtttcaaaaa aggtgttatc tttgagggtg aattttttaa cagaagggct aagagtgtaa
    11161 tcgtaacgac aattttttaa ctgaatgatt ttttcaaatg ccatatggct aacctccgat
    11221 aatttctttt aaggtttttg cgagggtttg taggtcttca acggtatttt gtggcgacaa
    11281 actgatgcga agggattcct tcaagcgttc tgaatttgcg ccatacatgg ctttaagaac
    11341 atggctggat tggacaacgc ctgcagtaca ggctgagcca gtagagattg aaattccagc
    11401 taaatctagc cgaaggagta agaggtcatt tttctgacca ggaaatccaa tattgagaac
    11461 ataagggaga tgatgttttc ctctattcaa gtaatactga atgccctcca gctctgccag
    11521 aaaggcagtt tctagatttt gtacatgttg aaaatgttct tcttgttttt ctaggtcttc
    11581 ttttagggct gcaaccatgc ctacgatggc aggcagattt tcagttcctg cacgtttttt
    11641 ctgttcctga tctccgccat gtagatagga atcaaagtcc atgctagatg cgtagagaaa
    11701 accgattccc ttaggaccat ggaatttgtg ggcagaagca gtgagaaaat caatgcccaa
    11761 ttcttctgaa tgaattggga ttttaccaat agcctgaact gcatcaacat gataggcagc
    11821 agggtgttgc ttgagtattt ggccaatttc agcgatggga agtaggtttc ctgtctcatt
    11881 attggcaaac atagtagaaa ccaaaatcgt atcgtcacgt aaagcctttt gaatttgctg
    11941 ggctgtgatt tcttgatttt ctggctggat aatggttgct tcaaacccaa agtgttgaac
    12001 caagtaatca attgtttcaa ggacagcatg gtgctcgatg gcagttgtga tgatatgttt
    12061 tccttgttct tggtgacgaa gacagtagcc aatgatggta gtattattgc cttcagtccc
    12121 accagaagtg aaaaagatat gttgaggttt tgtccttagt aactgggcta gttcctgacg
    12181 ggcttctcgc aagagtttgc cagcttgacg accatgacca tgaatactag aaggatttcc
    12241 gtgggtttct tgcataacct tggtcatagc tgaaatagca actgctgaca taggagtcgt
    12301 tgcagcattg tccaaataaa tcaaagaatc accttatttc tttttattgt aggcaaagag
    12361 tgggctgact ggttttcttt cgtgaatacg gacgatagca tcaccaatta actcactagc
    12421 agtgatgtag catacatttt taggagtttt ttcttttgtt gctactgaat cagtcacaag
    12481 aatttcttta atattagtat tgtcaagaag ctcagcagct ccctcgacga agagaccgtg
    12541 gctagaaaca gcataaattt ctgtagctcc ttcacgttca acgattttag aagcttcaga
    12601 gaaggtacgt cctgtattta aaatatcatc aatcaagata gctttcttac cttcaacatc
    12661 accaataata taaccttcgt tacgagttgc atcgtcttga gggtagtcga taatggcgat
    12721 aggagcatca agatattcag ccaggctacg cgcacgtttg acacctgaat ttttagggct
    12781 aacgacaaca acatctgaac caagcaatcc tttatcgcag taatgttttg cgaatagggg
    12841 aacagtgaaa agattatcca ctggaatatc aaagaaacct tgaacctgaa cggcatgcaa
    12901 atcaagagtc aggatacgat caactccagc cttaaccagc atattggcaa ctagttttgc
    12961 tgtaagtggc tcacgaggac aagcaatgcg gtcttgacgt gcatagccaa aatatggaag
    13021 gacaacgttg atactgtggg cacttgcacg cacacaagca tcgaccatga ttaacaattc
    13081 cattaggtgg ttgttgacag ggaaacttgt tgattggatg atgtaaacat cataaccacg
    13141 gacactttct tcgatattta cttggatttc tccgtctgaa aattgacgtg atgatagttt
    13201 tccaagtggg acaccaacag cttgggcaat tttttgtgca atctcttggt tagagttgag
    13261 tgcgaaaagt ttcatgtttt ttctatctga cattatagac cgtcctctgt aaactttata
    13321 aatcctagtt atatttacct tacatatatg aactgggatt tgtgtatttt tatcttttct
    13381 attttaccaa aaaatggaga ttatttcagc tatttttcat acttttgaca aatcgaacca
    13441 attttgaagg agctttttgg taggaaatct gatttttctc taaaaattgt cggaaatcct
    13501 gtttgccttg ctcatgattt tccacttcaa gctccaattc gtaatctgtc atatcaaagt
    13561 atcggctctg atccagtgcc atgagaccaa tagctgtttt catttcatag cgaagcgttg
    13621 ttagacaacc aagaacctgc cagttcttac tttggatacc atgtttcgcc aattcatcca
    13681 gtactagccc ttgaggaagt tcttccttac tcagatagtt ctcagcatct tttagttgca
    13741 atttttggtt gtattccatg tttccaacac tctgcgggac tttgagtgtc aactcagccc
    13801 agtcttcaaa ggttcgaatg cgcatagcga ctttcttttc tcgcagttca aaatcaggcg
    13861 tgtcgatgta gtaatttgtt tgaagaacag gagtgacacc tgtgaactgg tcttttagac
    13921 gattgtattc atcttttttc aatagtgttt tcaattcaat ttctaaatgt ttcatttttc
    13981 ttaccttttt ttatcgttga aagcggattt atggtataat aagcattgta tttattgtat
    14041 atgaatctgg agaaaaaatc aaagatattt ttgacggata atatgagaac aagggagaat
    14101 atatgacctt agaatgggaa gaatttctag atccttacat tcaagctgtt ggtgagttaa
    14161 agattaaact tcgtggtatt cgtaagcaat atcgtaagca aaataagcat tctccaattg
    14221 agtttgtgac cggtcgagtc aagccaattg agagcatcaa agaaaaaatg gctcgtcgtg
    14281 gcattactta tgcgaccttg gaacacgatt tgcaggatat tgctggctta cgtgtgatgg
    14341 ttcagtttgt agatgacgtc aaggaagtag tggatatttt gcacaagcgt caggatatgc
    14401 gaatcataca ggagcgagat tatattactc atagaaaagc atcaggctat cgttcctatc
    14461 atgtggtagt agaatatacg gttgatacca tcaatggagc taagactatt ttggcagaaa
    14521 ttcaaattcg tactttggcc atgaatttct gggcaacgat agaacattct ctcaactaca
    14581 agtaccaagg ggatttccca gatgagatta agaagcgact ggaaattaca gctagaatcg
    14641 cccatcagtt ggatgaagaa atgggtgaaa ttcgtgatga tatccaagaa gcccaggcac
    14701 tttttgatcc tttgagtaga aaattaaatg acggtgtagg aaacagtgac gatacagatg
    14761 aagaatacag gtaaacgaat tgatctgata gccaatagaa aaccgcagag tcaaagggtt
    14821 ttgtatgaat tgcgagatcg tttgaagaga aatcagttta tactcaatga taccaatccg
    14881 gatattgtca tttccattgg cggggatggt atgctcttgt cggcctttca taagtacgaa
    14941 aatcagcttg acaaggtccg ctttatcggt cttcatactg gacatttggg cttctataca
    15001 gattatcgtg attttgagtt ggacaagcta gtgactaatt tgcagctaga tactggggca
    15061 agggtttctt accctgttct gaatgtgaag gtctttcttg aaaatggtga agttaagatt
    15121 ttcagagcac tcaacgaagc cagcatccgc aggtctgatc gaaccatggt ggcagatatt
    15181 gtaataaatg gtgttccctt tgaacgtttt cgtggagacg ggctaacagt ttcgacaccg
    15241 actggtagta ctgcctataa caagtctctt ggcggtgctg ttttacaccc taccattgaa
    15301 gctttgcaat taacggaaat tgccagcctt aataatcgtg tttatcgaac actgggctct
    15361 tccattattg tgcctaagaa ggataagatt gaacttattc caacaagaaa cgattatcat
    15421 actatttcgg ttgacaatag cgtttattct ttccgtaata ttgagcgtat tgagtatcaa
    15481 atcgaccatc ataagattca ctttgtcgcg actcctagcc ataccagttt ctggaaccgt
    15541 gttaaggatg cctttatcgg tgaggtggat gaatgaggtt tgaatttatc gcagatgaac
    15601 atgtcaaggt taagaccttt ttaaaaaagc acgaggtttc taagggattg ctggccaaga
    15661 ttaagtttcg aggtggagct attctggtca ataatcaacc gcaaaatgca acgtatctat
    15721 tggacgttgg agactacgtt accattgaca ttcccgctga gaaaggcttt gaaaccttgg
    15781 aggctattga gcttccatta gatattctct atgaggatga ccactttcta gtcttgaata
    15841 aaccctatgg agtggcttct attcctagtg tcaatcactc taataccatt gccaatttta
    15901 tcaagggtta ctatgtcaag caaaattatg aaaatcagca ggttcacatt gttaccagac
    15961 tagataggga cacttctggc ttgatgctct ttgccaagca cggttatgcc catgcacgat
    16021 tagacaagca gttgcagaag aaatctatcg agaaacgcta ctttgctttg gttaagggag
    16081 atggacattt ggagccagaa ggggaaatta ttgctccgat tgcgcgtgat gaagactcca
    16141 ttattaccag acgggtggct aaaggtggaa aatatgccca tacttcctac aagattgtag
    16201 cttcttatgg aaatatccac ttggtcgaca ttcggctgca cactggtcga acccaccaaa
    16261 ttcgagtcca cttttctcat attggttttc ctttgttggg cgatgatttg tacggtggta
    16321 gtctggacga cggcatccaa cgtcaggccc tgcattgcca ttacctatcc ttttatcatc
    16381 catttttaga gcaagatttg cagttagaaa gtcccttgcc ggatgatttc agcaacctta
    16441 ttacccagtt atcaactaat actctataaa aactgtttca gagtataatt attatcttaa
    16501 aggagaaaac tcatggaagt ttttgaaagt ctcaaagcca accttgttgg taaaaatgct
    16561 cgtatcgttc tccctgaagg ggaagagcca cgtattcttc aagcgactaa acgtttggta
    16621 aaagaaacag aagtgattcc tgttttgtta ggaaatcctg aaaaaattaa aatttatctt
    16681 gaaattgaag ggatcatgga tggttatgag gtcatcgacc ctcaacatta tcctcaattt
    16741 gaagaaatgg tttctgcctt ggtggagcgt cgcaagggca aaatgactga agaagatgta
    16801 cgcaaggttt tggttgaaga tgtcaactac tttggtgtga tgttggttta cttgggcttg
    16861 gttgatggaa tggtgtcagg agcgattcac tcaacagctt caacagttcg tccagctcta
    16921 caaatcatca aaactcgtcc aaatgtaact cgtacttcag gagccttcct catggttcgt
    16981 ggtacggaac gttacctatt tggagactgt gccattaaca tcaatccaga tgcagaagcc
    17041 ttggctgaaa ttgccatcaa ctcagcaatc acagctaaga tgtttggcat cgaacctaaa
    17101 attgccatgt tgagctattc tactaaaggt tcagggtttg gtgaaagcgt tgataaggtc
    17161 gttgaagcaa ctaaaattgc tcacgacttg cgtcctgacc ttgaaatcga tggtgagttg
    17221 caatttgatg cagcctttgt tcctgaaact gcagctctga aagctcctgg aagtacggta
    17281 gctggtcaag caaatgtctt catcttccca ggtatcgagg caggaaatat cggttacaag
    17341 atggctgaac gcctgggtgg ctttgcggct gtaggacctg ttttgcaagg tttaaacaag
    17401 ccagttaatg atctttctcg tggatgtaat gcagatgatg tttacaagtt gaccctcatc
    17461 acagcagctc aagcagttca tcaatagtga aaactataaa gcgatatact atgctatact
    17521 gtagttatga aactatgtac gaaaagcact gccattaatt cctgagaact aaattactga
    17581 ttggtgtcaa aaaggaaaac ttccaagcga tgatatcctg tctatacacg acctatagaa
    17641 atctgtaata tgtatgtccg taaaacgata atttcccttt ttgattttaa atgagtatga
    17701 aaagagaatt ttttcatgag aaaacgcata atattaagtg tcttgacacc tgctattatg
    17761 cgttttctgg ctctttgtca actgtagtgg gttgaagtca gctaagctcg agaaaggaca
    17821 aatttcgtcc tttctttttt gatgttcaga gcgataaaaa tccgtttttt gaagttttca
    17881 aagtttcgaa aaccaaaggc attgcgtttg ataagtttga tgagattatt ggtcgcttcc
    17941 aatttggcgt ttgaataggg tagttgaagg gcgttgacga ttttctcttt gtcctttaga
    18001 aaggttttaa agacagtctg aaaaagagga tgaacctgct ttagattgtc ctcaataagc
    18061 ccgaaaaatt tctctggttc cttattctgg aagtgaaaaa gcaagagctg atagagttga
    18121 tagtggtgtt tcaagtcttc cgaataactc aaaagcttgt ttaaaatctc tttattggtt
    18181 aagtgcatac gaaaagtagg acgataaaat cgcttatcac tcagtttacg gctatcctgt
    18241 tgaatgagtt tccagtagcg cttgatagcc ttgtattcat gggattttcg atgaaactga
    18301 ttcatgattt gaacacgcac acgactcata gcacggctaa gatgttgtac aatgtgaaag
    18361 cgatcaagaa cgattttagc attcgggagt gaaacagtct gggagactgt ttcagcctga
    18421 gcctagaaat ttgaaagcga agctgtttag ccaagccata gtaagggcta aacatatcca
    18481 tagtaatgat tttgacgcga catcggacgg ctctatcgta gcgaagaaag tgatttcgaa
    18541 tgatagcttg tgttcttccc tcaagaacag tgatgatatt gagcttgtca aaatcttgcg
    18601 caataaagct catctccatc tcccgattga aacagtcact acccggactg tttcaacgtc
    18661 ccaagacata atctcaggaa gacaagaaaa atcatgttta aagtgaaaat cattgagctt
    18721 acgaataaca gttgaagttg agatgaaaag ctgatgggca atatcagtca tagaaatctt
    18781 ttcaattaac ttttgagcaa tcttttggtt gatgatacga gggatttggt gatttttctt
    18841 ta
//
LOCUS       DAVHIU010000028        18764 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVHIU010000028 DAVHIU010000000
VERSION     DAVHIU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.030"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            59..412
                     /locus_tag="VVN06_000854"
     CDS             59..412
                     /locus_tag="VVN06_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562432.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(472..663)
                     /locus_tag="VVN06_000855"
     CDS             complement(472..663)
                     /locus_tag="VVN06_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU5562433.1"
                     /translation="MTSKLTASTCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIE
                     IKQFKVTVITEPDAKTQSR"
     gene            1185..1355
                     /locus_tag="VVN06_000856"
     CDS             1185..1355
                     /locus_tag="VVN06_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU5562434.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            1741..2958
                     /gene="mef(A)"
                     /locus_tag="VVN06_000857"
     CDS             1741..2958
                     /gene="mef(A)"
                     /locus_tag="VVN06_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU5562435.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            3078..4541
                     /gene="msr(D)"
                     /locus_tag="VVN06_000858"
     CDS             3078..4541
                     /gene="msr(D)"
                     /locus_tag="VVN06_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU5562436.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(4659..4958)
                     /locus_tag="VVN06_000859"
     CDS             complement(4659..4958)
                     /locus_tag="VVN06_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562437.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(4945..5313)
                     /locus_tag="VVN06_000860"
     CDS             complement(4945..5313)
                     /locus_tag="VVN06_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562438.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            complement(5326..5661)
                     /locus_tag="VVN06_000861"
     CDS             complement(5326..5661)
                     /locus_tag="VVN06_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU5562439.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            complement(5658..5903)
                     /locus_tag="VVN06_000862"
     CDS             complement(5658..5903)
                     /locus_tag="VVN06_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562440.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            complement(6267..8186)
                     /gene="tet(M)"
                     /locus_tag="VVN06_000863"
     CDS             complement(6267..8186)
                     /gene="tet(M)"
                     /locus_tag="VVN06_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU5562441.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(8202..8318)
                     /locus_tag="VVN06_000864"
     CDS             complement(8202..8318)
                     /locus_tag="VVN06_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU5562442.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(8563..9495)
                     /locus_tag="VVN06_000865"
     CDS             complement(8563..9495)
                     /locus_tag="VVN06_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5562443.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(9492..10493)
                     /locus_tag="VVN06_000866"
     CDS             complement(9492..10493)
                     /locus_tag="VVN06_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU5562444.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(10490..12667)
                     /locus_tag="VVN06_000867"
     CDS             complement(10490..12667)
                     /locus_tag="VVN06_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5562445.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKAERASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(12670..15117)
                     /locus_tag="VVN06_000868"
     CDS             complement(12670..15117)
                     /locus_tag="VVN06_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5562446.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(15101..15607)
                     /locus_tag="VVN06_000869"
     CDS             complement(15101..15607)
                     /locus_tag="VVN06_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5562447.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            complement(15582..16079)
                     /locus_tag="VVN06_000870"
     CDS             complement(15582..16079)
                     /locus_tag="VVN06_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU5562448.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(16196..16417)
                     /locus_tag="VVN06_000871"
     CDS             complement(16196..16417)
                     /locus_tag="VVN06_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562449.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            16597..17844
                     /locus_tag="VVN06_000872"
     CDS             16597..17844
                     /locus_tag="VVN06_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5562450.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            complement(18101..>18764)
                     /gene="erm"
                     /locus_tag="VVN06_000873"
     CDS             complement(18101..>18764)
                     /gene="erm"
                     /locus_tag="VVN06_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HEU5562451.1"
                     /translation="NLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSE
                     KLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIYLIVE
                     EGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDK
                     YWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTITYEQVLSIFNSYLLFNG
                     RK"
BASE COUNT         5656 a         3594 c         3518 g         5996 t
ORIGIN      
        1 ttttctatac ctgtttttaa ttcagtagga acaataaaat gtatagaggt ggtctactat
       61 gcgtaaaaaa gaagataaat atgattttag agcctttggt ttagccatta aagaagctcg
      121 attgaaacga ggtttaactc gtgaacaagt gggagcattg attgaaattg acccacggta
      181 cttaactaat attgaaaata aagggcaaca ccccagcata caagttcttt atgaccttgt
      241 atcgttactt catgtttccg ttgatgaatt tttcttacct gctaataact tggtaaaaag
      301 cacccgacga ttacagatag agaaatacat ggatagcttt acagacaaag aactatcctt
      361 aatggaatct ttagccagcg gtatcaacga agcaagaaac atcgaagact aattaaaaga
      421 atccatacat aacggaaaga gccgataaaa tgagattgta ttaatctcat tttatcggct
      481 ctgcgtcttt gcgtctggct ctgtaatcac agttactttg aactgcttta tttcaattaa
      541 attttcttgt ctgcatttcg gacaatagag ggggaatttt tttaattcag tatcttccct
      601 tatctttaat cgtgttttat ttccacatac aggacatgtt gaggcggtaa gtttgctagt
      661 caaggagtaa aacgacgaag attagcattt acttccgccc atgcgatagc tgtccgtgat
      721 tgacaagtgc tagcacgcag acagaacgga gatagcgaac cgctgagtgt gtcgctctgc
      781 tcgtaaaagc ttagaaacct ttgaacgaaa gggataatga aagccttgat tgcaaggctt
      841 tttgctttat ggtgggtaag tatcagagtg agaaaatttt tggaatgagt agaagtgata
      901 gctagaaatt atcagtttct atttccattt accctgtggg tacgtgtttg tttccattga
      961 caaggagttt gtgggaatag aaatgtaccc accttgtttg aatcaagtga agtgtagttg
     1021 aaggaaatct gttgaaagca atacttcatt ttaccgaata agtaataatt taggcaactt
     1081 caaatcgatt aaaaaaaact attttaaagg ttaagagtag acaaaaattg tccactcttt
     1141 tttgcaaact caatttatca ataaatgaaa tgagggaatg taaaatgaaa tattttgagg
     1201 ttgagttaga aaatcctgat gaatttttaa aactacaaac agaagatttt gtgaaagcta
     1261 atcgcttgct actaaggaag ataatccaga gcgttacagt ctatgaagaa aacttcgtca
     1321 tatcctttaa atctggcatc gaattggaag tatgagtctc attccataac ttttatattg
     1381 aacatatcat cttgttgtgt tatactataa attgatataa acaaagatgt aggaggaacc
     1441 gaaactatga cagcctcaat gcgtttaaga taagctggca ataaaaaaag cagaatctat
     1501 acccgatgat aggctttttt gttgtgctta tttatacgat attgagcatt cattagttac
     1561 ggtgaggata ttggttattt aactatacct ttatttaact atgtctttaa tatgaatgtt
     1621 tccaaattgt atgtatgcag accaaaagcc acattgtggg gtttggcctg cattttttat
     1681 tgcctagaat gctattcaaa atagaaattc aagcaaaata atatgcagga gataatataa
     1741 atggaaaaat acaacaattg gaaacgaaaa ttttatgcaa tatgggcagg gcaagcagta
     1801 tcattaatca ctagtgccat cctgcaaatg gcgattattt tttaccttac agaaaaaaca
     1861 ggatctgcga tggtcttgtc tatggcttca ttagtaggtt ttttacccta tgcgattttg
     1921 ggacctgcca ttggtgtgct agtggatcgt catgatagga agaagataat gattggtgcc
     1981 gatttaatta tcgcagcagc tggtgcagtg cttgctattg ttgcattctg tatggagcta
     2041 cctgtctgga tgattatgat agtattgttt atccgtagca ttggaacagc ttttcatacc
     2101 ccagcactca atgcggttac accactttta gtaccagaag aacagctaac gaaatgcgca
     2161 ggctatagtc agtctttgca gtctataagc tatattgtta gtccggcagt tgcagcactc
     2221 ttatactccg tttgggattt aaatgctatt attgccatcg acgtattggg tgctgtgatt
     2281 gcatctatta cggtagcaat tgtacgtata cctaagctgg gtaatcaagt gcaaagttta
     2341 gaaccaaatt tcataaggga gatgaaagaa ggagttgtgg ttctgagaca aaacaaagga
     2401 ttgtttgcct tattactctt aggaacacta tatacttttg tttatatgcc aatcaatgca
     2461 ctatttcctt taataagcat ggaacacttt aatggaacgc ctgtgcatat ttctattacg
     2521 gaaatttcct ttgcatttgg gatgctagca ggaggcttat tattaggaag attagggggc
     2581 ttcgaaaagc atgtattact aataacaagt tcatttttta taatggggac cagtttagcc
     2641 gtttcgggaa tacttcctcc aaatggattt gtaatattcg tagtttgctg tgcaataatg
     2701 gggctttcgg tgccatttta tagcggtgtg caaacagctc tttttcagga gaaaattaag
     2761 cctgaatatt taggacgtgt attttctttg atcggaagta tcatgtcact tgctatgcca
     2821 attgggttaa ttctttctgg attctttgct gataaaatcg gtgtaaatca ttggttttta
     2881 ctatcaggta ttttaattat tggcattgct atagtttgcc aaatgataac tgaggttaga
     2941 aaattagatt taaaataaac aatattggag gaatatttat gtatcttatt ttcatgtaac
     3001 tcttcctgct aaaatcgcag ggttttccct gcatacaagc aaatgaaagc atgcgattat
     3061 agacaggagg aaatgttatg gaattaatat taaaagcaaa agacattcgt gtggaattca
     3121 aaggacgcga tgttttagat ataaatgaat tagaagtata tgattatgac cgtattggtt
     3181 tagtaggagc aaatggtgct ggaaaaagca ctttactcag ggtactttta ggagaattaa
     3241 ctcccccagg atgtaaaatg aatcgtctgg gtgaacttgc ctatattccc cagttggacg
     3301 aagtaactct gcaggaggaa aaagattttg cacttgtagg caagctaggt gttgagcaat
     3361 taaatataca gactatgagc ggtggtgaag aaacaaggct taaaatagca caggccttat
     3421 cggcacaggt tcatggtatt ttagcggatg aacctacgag ccatttagac cgtgaaggaa
     3481 ttgattttct aataggacag ctaaaatatt ttacaggtgc actgttagtt attagccatg
     3541 accgctattt tcttgatgaa atagtagata aaatatggga actgaaagat ggcaaaatca
     3601 ctgagtattg gggaaactat tctgattatc ttcgtcagaa agaggaagaa cgtaagagcc
     3661 aagctgcaga atacgaacaa tttattgcgg aacgtgcccg attggaaagg gctgcggagg
     3721 aaaagcgaaa acaggctcgt aaaatagaac agaaggcaaa aggttcttca aagaaaaaaa
     3781 gtactgaaga cggagggcgt ttagctcatc aaaaatcaat aggaagtaag gaaaaaaaga
     3841 tgtataatgc tgctaaaacc ctagagcaca ggattgcggc cttaggaaaa gtagaagctc
     3901 cggaaggcat tcgcagaatt cgtttcaggc aaagtaaagc attggagctc cataatccat
     3961 accctatagt cggtgcagaa attaataaag tatttgggga taaggctctg tttgaaaatg
     4021 catcttttca aattccgtta ggagcaaaag tggcgttaac tggtggtaat ggaatcggaa
     4081 aaacaacttt aatccaaatg atcttaaacc atgaagaagg aatttctatt tcgcctaagg
     4141 caaaaatagg ttactttgca cagaatggtt acaagtacaa cagtaatcag aatgttatgg
     4201 agtttatgca gaaggattgt gactacaata tatcagaaat tcgttcagtg ctagcatcta
     4261 tggggttcaa acagaacgat attggaaaaa gtttatctgt tttaagcggt ggagaaatta
     4321 taaaattgtt gcttgctaaa atgctcatgg gtagatataa catcctaata atggatgaac
     4381 ccagtaactt ccttgacata ccaagtttag aggctttgga aatactaatg aaggagtaca
     4441 ccggaactat cgtgtttatc acccacgata aacgattact cgaaaatgta gcagatgtag
     4501 tttatgaaat tagagataag aaaataaatc tgaaacatta aatttaaggt agtcgctggt
     4561 cagtatagtc tgttctggtt ggcgactcca ttgttaaaga gtataaagac tttagatttt
     4621 atgaatatta aaaataggaa cagtcaattg aactgctcct atttttctgc taaatatatt
     4681 gtagttttct tatatgtata atgatagatt agcggattct catctacggt acttacttca
     4741 aatatgaaga agtgatcgcg gttatctctg gacttttcct tattgaggac aaagtaattc
     4801 ttacgtgaag tcgccattgt ttttaggata tcatcagtta ggaaggtcaa tggaatattc
     4861 atgttagagt agcggtagaa gtcacgttca aaatcttggt agctctcgct ataatagtcc
     4921 atttgtaggt gattacgctg aaactcaagc tgattcatag agcacctcct cgacaagttc
     4981 aatactaata atgtctttta atttcaaatt gatgtgacct gttgtagttt ttatcaaaat
     5041 gaaatctttg gtcagacttg gtattgttcc agtgtaggaa acacgcttgt ttttttcaat
     5101 cacttgaatg cgtgtgcgta gctgcccggc gtatacttga ctgaggagta ataatttctt
     5161 ctctagtgat aagtcagaca tgtacgttac tttgtttgta tcatcagaga gtgctgatgc
     5221 atgttcagat aggaaaaagc ccatccattt ttgcatcttt gtatcctggt actctcttgc
     5281 tgattgaaat ggtaaatatg aacggtcaat catatcaaat cctttctatg cagaggcaag
     5341 ggtattttta tcaaattgaa tcgtaaaacc ttgaattccc ccacctgtgt aacattcttt
     5401 aaagcgattg attacctcag tatagattat cacagatgag cttgttggct taatgctaaa
     5461 tgtaaattcc aatggtaatc ggttttcaga tttagcatgt actagtcgta tcgatatttc
     5521 agttgttttg agttttctct gacgaagttt tgaagttgct gtttcaacga ttccatgtaa
     5581 aaatctttca agcatttcaa tatcattaca tcctttgcta cggatttctg aaaattgtac
     5641 tgtatttttt tcttgtttca ttttaatccc tccaatccac ccgcggaatg accaccgata
     5701 agtttactgc gttcaatatt tctggaacct tcagttagga cggttccttt ttgtatggct
     5761 aaaaaaccaa actgttctct gacaacatca atagctgtct gaagtctatt atctttttca
     5821 atttgttcta catcatcaaa gagtgatagt agagtatagc tttcatctac gaagccacta
     5881 taagatacac caatttgtct cactgcacca gaggtgtatt ttttcggaat aatacaagta
     5941 catgactcac cattgttttg gggagatttg cgggttcaat tttattctga gcatttatag
     6001 attttttcat ctcagtccta gaatagccaa tatgaataga aacgacagta gtcaatacta
     6061 gggctactgt tccgttccac agtatcattt aaaaatcatt ttcacaccct ttcgtctatt
     6121 agtatagaag aaagctctca gcacacaata tccacttgta gtttataata actatctcct
     6181 cctttacact ttaattcaaa tctttattaa aaaatatttc atcttattta acaagaaacc
     6241 atatttatat aacaacataa aatacactaa gttattttat tgaacatata tcgtacttta
     6301 tctatccgac tatttggacg acggggctgg caaacaggtt caccggtagt aacatggtac
     6361 ccttttaact ctgttaaaca aacactacgt ccatttgtaa agaaagttaa atcactacga
     6421 tattcttgaa tacaccgagc agggatttct ccactaagaa tgacctcatt atttttcaat
     6481 tgagtgtcta cgatgttcgc acaatattta ggagcatcgg tgtatgctcg tgaaagatat
     6541 tcctgtggcg cataaatttt aaaactaaga tatggctcta acaattctgt tccagctttt
     6601 tttaagactt gttccaatac aataggagca agcatccgaa aatctgctgg ggtactaaca
     6661 gggctatagt ataagccata cttaaaacag attttacagt ccgtcacatt ccaaccatac
     6721 aatccttgtt cacagccata gcgtatccct tccataactg cattttgaaa tgattgattt
     6781 aagtatccaa gagaaaccga gctctcatac tgcattccac ttcccaacgg aagcggtgat
     6841 acagataaac caatggaagc ccagaaagga ttcggtggaa cttcgatgtg aatggtatac
     6901 tctgcttttt ttaacggtct ttccatataa atgactgtag gctcttttat ttctatctcc
     6961 acatgatact tttcttgcag cagagcacaa gtcacttcca tttgtacttt ccctaagaaa
     7021 gaaagtatga tttcatgtgt cgcagaatcc acataatatc gcagaagcgg gtcactgtcg
     7081 gagatttcta aaagtgcatc aagtaacatt tccctttgtt gaggtttgct cggttcaaca
     7141 gtcgtttgca gcagagggag gggattttca attctctctc tctgtggcaa tagctttgta
     7201 tctccaagaa cactatttaa cttcaaaaac tcattctgca aaataacaat ttccccggaa
     7261 taagccttat cgattttaca taattcacca tttattgaag tatacatttc tgtaattttt
     7321 attttttcct tttccgatat tctaaccgaa tctcgcaaat gcagtacgcc actataaaga
     7381 cgtatatatg caagacgctg tcttttttcc gaatactcaa ttttgaaaac ttttccgcaa
     7441 agttcagact gacctcgatg tgttgatgaa taaaatttat tcgtaatcac ttctataagg
     7501 ttatcaatcc ctatattgtt ttttgcactt ccgtgataaa cagggaacag ggaacaatta
     7561 tgaaatctta tgctttcctc ttgttcgagt tccaatgctt ctaatgattt accggacata
     7621 tatttctcta aaaggtcatc gtttccctct attaccgtat cccattgttc agattcggta
     7681 aagttcgtca cacacatatt aggatacagt tctaccttct gtttgattac aatttcggca
     7741 gaaagtttct ctttaatatc ctgataaacc gttgataaat caattccatt ttggtcaatc
     7801 ttattgataa aaaagattgt gggaatcccc attttcctaa gtgcatgaaa taatatacga
     7861 gtttgtgctt gtacgccatc ttttgcagaa atcagtagaa ttgccccatc taaaactgat
     7921 aatgaacgat atacttctgc taagaaatcc atatgtcctg gcgtgtctat gatgttcacc
     7981 ttcgtatttt cccactgaaa agaggttatt cctgtctgaa ttgtaattcc tctctgacgt
     8041 tctaaaagcg tattatccgt cctcgttgta cctttgtcca cgcttcctaa ttctgtaatc
     8101 gctccactgt tatataataa gctttctgtt aaggtagttt ttcccgcatc aacatgagct
     8161 aaaactccaa tattaataat tttcatgtga ttttcctcca ttcaaaagcc caaaagggca
     8221 taaaaatccc agtgataaat actcttatca ctgggatttt tatgcataac cataggcata
     8281 caaagcatac agatattctc cggatacttt agaatcacat gataaaggta ttcttaaact
     8341 gggtacaaaa aactaagccc tcctaaaaaa ggacatccaa ttatttgttc gcactatcaa
     8401 attgacagtt tatttaagaa taccttgccg catatttatt aactcctttt aaatagatac
     8461 ttaaataata gcacgtaaga gcatatttgt aaaggaatct ccaatttttt atcaaagaga
     8521 gtacgtgatt acaaaatagc tgtaataatg taccaatatt tgttattcta taatcttcca
     8581 attactcccg ttcttttcaa gtaccaaatc aaattgagat acctgcgttg ctttggtctg
     8641 ctggtcgata tactccactg tcagcgatac cgtgacttga ttatccttac gattgtgaat
     8701 aggatttacc agttcttgaa agatgtactc ttttccgatt ggttttaata tcccgtcatt
     8761 cacatagtag gaaagttcac tggctgtcgc tgtaggatag agcttgaaga acgtcgttaa
     8821 aaactcattg atttcattgg ttgtaatgga atcaaccgtc ccctcacttt caatggcttt
     8881 tggtttataa cttgatttct taggtatgtt ggtaatggtc ggattcttaa ccagtaccat
     8941 atttccagaa ccatctacat agacactcac tatataagca gagtggacgg tctttgtatt
     9001 ttctccctct gtaatgagct ggtctacact gtaggttaca ttaaactcat tgtcgccagt
     9061 tggctctacc gtccatatct gaaatcctct tacagaagac gatacaggaa tatctttgcg
     9121 tactgtatca acattgagag cttgaagttc atctgtcaga tagcctttta gactttccat
     9181 tcgattatca atggacttat cggattgctc ccatgaatag tagactttcg caaagttctc
     9241 tacaaaattt tctacatgat gagtatcaac gtattccttt tctatgatag ttgtttcgtg
     9301 aatagtatga gtatctatag ctgtaaagtg cttgaatatc gcaaagctga aactaagccc
     9361 taaaagtacc cacaaggcaa tcacaacctt tttatgagga ttgaccttat agacacgagg
     9421 tttcttttcc tttggtatct gtttttcttt attctgattt tttctaaatt tcatcattaa
     9481 atcttccttt ctcattgttt gattcgtcct gctcccacta aatgctgttg ccagtagggg
     9541 cttgttaagt cggcataacc gattgggtcg cctgcatgaa acatacggtt attgccaagg
     9601 tatatcccaa catgagtaat ataagagcca gcgttatagg tagaatgaaa gaaaaccaaa
     9661 tcgccagctt gtgcttccga tagtgggata tgctgggtca catcatattg ctgttgtgcg
     9721 gttcgtggta agttaattcc agcttttcca tacgtccatt gtgtcagtcc gctacaatca
     9781 aaagaagtag tcggggaagc tccaccgtaa acgtatcgcc agccctcata tttcagtgct
     9841 tcgtccatga tggcttgtac cgtatcatca tcaaactctg ttgtgacaag atactgcgtt
     9901 accagttgca cataaaacat attgccatag ttgtatcgcc agcccccatt gataggtatg
     9961 gctatgggat tggggtaaga cactttttcg ccacctgaat actcttttga gaaactttga
    10021 gccagttcaa aggtatattt atttccacga ttagccacat accctaagaa accaccacca
    10081 taattgtagg actggataac cgattctaaa tctacactga gcctttcgct actggctaat
    10141 aattcactga aatacttcac accttgctta atggattctt ctgtactcaa tgaattaggt
    10201 ggaagaccga gggattccga ggactgcata acatcttccg cagtaccgcc cgattccacc
    10261 tgtataatcg caagaagtat gttgacatat tcttcaacgc catattcttt ggcatatttt
    10321 tctaccatag gcttatgagc cagcacttct gcggaaacat tcacacctcc ataatgaata
    10381 ttggaaattc cgctgtcctg ttcatctgaa aataaaatgg caacaaacag aagcagtgag
    10441 aagaccatca agaataatcc agaaccacca atcactaaag ttttcaactt catggtttct
    10501 taccgacttt cttaatggtg gcggttttga ttggtggtct acttcttgta ttttgtagtg
    10561 gtactctttg aacagtagac ggacgttctt ttgtgattgg acgttgtgaa gttctatctg
    10621 ctgtagtggt tgaagttgct ggcttttgaa cggttttttc ttgaacggta ttgccttggc
    10681 gttccacttt tggacttgaa aaatcggact taactgctgg acgctcttgt ttggcttgtt
    10741 gagattcctt atatgaagtc tgaatattag actgttttga ggtctgttca tcatgatatt
    10801 gttcttgtct tgtagtcggt ctttcatgaa cagaagaagc aggctgtttt ttctgtttga
    10861 cctgttccat ttcagagcga cgcttcgcaa tggtttttcg cctttgttcc tgctgttcct
    10921 tgcgtccact ggctctttcc gctttggttt gagaaatact actggttaaa tcacggacat
    10981 tctcttttac tttggatttt ccttgatata ctgcatatct tgcattggtc ggcaaatctt
    11041 taacctgttc tttcaaacca ctagcagtgt ctaccattct gtctttggta tcagctactg
    11101 taccgatggt ttgaccgata cgttttccaa gtgttgattt ttcctttccg tctggtcggg
    11161 agtgatctgc ttgtgtcctt gcagaactcc ccgaacccga ctgtcctttt ttacctgtaa
    11221 caatggcaga cccagcccct agagtagtca tggaacgtcc aagtttccgc tgtagacggt
    11281 gcatgtgagc gtgcataagc atacgaggtt ttctcatcac acgacttccc acactttgag
    11341 aatcgttact ctgtagagaa aacatactca ttaaatcgcc cagcttgaag tagattcctg
    11401 caaaggtcac aatctgtaga aaagcaatca aaaagaacgg ataaccagcc gataaggtat
    11461 agagcatggt tgaaatacta aatgctgtcg taataatcaa tgtgattcca gctcgtgtca
    11521 aaatggtatt aaagagcttt gttatggctc gttttgacat accatcaaat gatggaatca
    11581 tgcttaaaat aaagctcaca ggcagaaaca tagcatagat gataaaaagt acctgcgaga
    11641 aaatcatgat tcctgttaat aggaatacaa atatggaaat cccaatattg aagacaaata
    11701 ggaagaagac tgtacctaaa cggttaatgg tctttgtaat ggttagattg gtattgcttc
    11761 tgtcttcaat ttcttccgca acaatttttt ctctgtcttc gccattgttg gaatctgggc
    11821 tggtggagag caggctttcc acacggtcaa taccgatact ttcaatgtct gaactgttgt
    11881 attgaagcag tagccacggt tgctgaacct gtatggaaaa caggctatct ctgattaagt
    11941 ccacgctgtc cttgccttga ctatcggaat ggggcatgac aatcttcgtg ccaagtgata
    12001 aactggcatt actgatgtct gatgaaaagt cattgatttt tttaatgtag tcgggagcgt
    12061 aggcaataaa ggaagccgat aggataaaca ccagcacaaa attcataatg gcatgaattg
    12121 cctttgtggt ttctctcttt atcagtcccg tataggcaac ataaacccca agaaccaaaa
    12181 tcaagagtaa gaggaatcca acatagaaac cctctgttga aaatccgttt gcactcacac
    12241 cagctaaggt ctgcatattc ttaccaatgg aatctgctgt agcggaaatg aagtctaagg
    12301 aataggcttc ctgtactaag taacctgtcg cattggaaac atacaaactg attgtccaaa
    12361 taaaattggt aatggcatat agtccataca tgacctgttt tccaatcccg tccgaccagt
    12421 tccacggaag ccagccccag ctattatcca cataaaaatc cagttgatag ttttcaagtg
    12481 ggtatcggct gtattcattt gccacattga ccgtatcatc taccaagccc gcagcttgaa
    12541 ccaccgttcc cagcatggct aaaagaaaaa tggcaatcac aagtgtgaaa gccactgtca
    12601 ttgccacttt acctagacgt ttcagcgtcc agtttgattt tattctgttt actattgatg
    12661 gtttcacatt tacacctctt ttcgcacagg tggtctggta tcaaaggcat ggagcagttc
    12721 ttcaaataca gggtggaact gtatcacacc gacacgacca tataaatcac tgataaggca
    12781 ttgcccgttt tccaaatcac gcaatcgctt ctgattgttt tcgtcctctg ggtctacacc
    12841 aaaaaaggct aaggtctttt taatctcgtt aaggtcagtg gaacgaaatg caaattttaa
    12901 gccgaggtta tttttcagtt tttcatctaa gaggtcgtct gtattttggg tcacgaaata
    12961 taccccagcg ttcatagcac gaccagcccg aaccagcttc atagatagtg tttttccttg
    13021 tgctacctgt aaaaagctcc atgcttcgtc taaatctaca atcttgaaaa tgcttcggtc
    13081 tgtatggata aagtctaaag caaaggtact aatgacaatc agcatagcaa cggataaaag
    13141 ctccatagtg gtatattcct caaaggaagt ttccttgtcg ggaagtacca agtccgcaac
    13201 ctgtataatg ttcagttgtt tttctaagct gatagactgc tccacataac cattactgaa
    13261 taataaatgt gcaaagtcat agtctgtaaa actttcgata tggtcggcta tactggtact
    13321 tagtggcgta ttctcaaccc gtaattcctc aatcactttc atcaaccctc gtacttcact
    13381 attggttact gcacgaatgg cttttctaag gattgggaag cgttccccat cacgagagga
    13441 aatccccgta aggaatgtca gaatatcaat agccagtgat tcagaatctt tgggattttt
    13501 cataatcaca taagggtcaa gtaagccttt gtttttctca tcagaagtca gagtgacgat
    13561 attgatttca tgggaaatct ctggcaaggt ttctttccat ctgccacgtt ctgcttttgg
    13621 gtctacaatc actgcttgtg ccccataaag cactgcataa tagacgataa ggttattcgc
    13681 aaaggattta ccaccaccca gcgaaccgac aaaggcagac gctaacgcat tggttactga
    13741 acccttaacc ccttgactgg caagagcagg tttcagatag acattgcgtc cagtatctaa
    13801 gctgtagcca acataaatcc cctcattttc acccagcatt tgagtagcac caaaacctaa
    13861 accagcgagg aaatcagagg tcacgtattg aatatagtca ttcatatatc ttttgctggc
    13921 aggtaaaaat tcttcatgta agcccagcat atccccaaaa ggtcgtacca gtttcacgct
    13981 caaatcatca tagaaatctt tcacttcatt acaacgacgt ttaagttcgt caaggtcatt
    14041 tgctgatacc cttacaacat aggacagctt atacatagat tccttgcttt ggtctaaatt
    14101 ggtttccagc tcgttcacac tttccagagc ttccgccaca ttagagctgg tttcattatc
    14161 actttgccat gcgtggttat ccaagtcttt cagttctttc tttttattgc ggacagtaga
    14221 tagggcttta cgattcgcta caatttccac attcattgac gtatcaatcg ggaatgtaaa
    14281 ttgctgttgc tggtagtaga agatttcaga ggacgggaag tccagttctc cgacaatgct
    14341 gttaatggta aagtaagcta catagacggt ttcatcttcc tgctggattt tcaaatatcg
    14401 ctgtttttct tccaccaaac agcgagtagg cttaatcaag tcatagtatt taatcagcgt
    14461 ttcattatcc agctttttct ttgatagatg gtactcatac tcttcatagg cagtgcctgt
    14521 ctgtccgtaa aggtgttcaa tcagatagcc gaagtcgtcc ttatctaacc tgcggatttt
    14581 gaaacgacga gagattttat tttctaagag cttttccatc ttctgaaaac gcaggatttc
    14641 atcattactc atactaacaa aatcgcccat cagcttatgg ttcacatcat agacaaaatc
    14701 agacaaagca ttttttgctt caacggtaag acttttcata gaaaactcct gatcgttgag
    14761 aagcaactta aagccgataa agaaacggta gttcacttga ttttcgccaa tcatggatat
    14821 taaagcgtct gtctgttggt cgattttgtc ataggcaacc gctttgagct tgccagtgac
    14881 ttcatttttg gaacgctctt gtgcagaacg tatgctggat tctgtactga tttgtaaagc
    14941 atgaattttg ccatcacgat tttgtgcgat aagctgtctg aaagaatcat gcacttgtat
    15001 tttctgttct ggacttagaa atgagtaatt gtaaggaaca agctcatagt aagcataaca
    15061 ttccccgtct ttattccaga cgagattgtt ttcaatgtat ttaattggat atgccataaa
    15121 attcactcct aactgctgta atggcttctt gtggctggtt tctgccaagc gttacttttt
    15181 ttcctgcata ggtcagcttt ggtcgcagtg cataagcaat gacagacttc aaaaatccat
    15241 aaggcttttt accatcaaaa gtttttgtag acataaacca tgtgaaagcc acaggaatcc
    15301 caaagtattt gagaaatgct ccctctatca tggaaagagg gggcaagttg ccaagtatca
    15361 tcactgcaaa gagtgacacg acaaaccatg tcatttgcgt aaaggttatg ggaaacggaa
    15421 gtctaaaatc attgatagaa tacagtacct tttccacaga ccagatactg gtatagcttc
    15481 gtattttctt catgtaatca atcctttcat aaaaaatagg ggtagctgat tgagccaccc
    15541 cgtaaaatag aaaatctgcc agtagtaatg taccgacaga tttaatagac gatttcaaaa
    15601 atcccatgat tggttgagat aaacgttcct gaaaggtcta aatcccgacc ataggcttga
    15661 taatcaatat agttttgaag actagctggt acttcgccta aagcacccgt ttcttcaatg
    15721 tagtagcgtg ccacgtcata catatcatca caatcggaat gaatgataat atcctcttga
    15781 tgttcgctta gttcttcaat gcttgaaaaa tgagtgagca gagcagatag ctccgattgt
    15841 aattcttcgg gtaattccga taccatttcc catagtcgat tgagttcgcc aatggaagtg
    15901 tattcgtcaa ccgtaaaggg taactcgtag tcatgaatgg cgtattcctc atattcatca
    15961 ttcaagccga ttttctcttt gacttcctca aagtcaatgg gaaaggtaaa ccacgcaccg
    16021 accaattcgc cctcattgta tttgcctaaa ttcgcaatat agacttgcat atcgtccata
    16081 tattcacgtc ctttctttgt agagattcaa aaatccctac cgcacttcgt ttggtgtacc
    16141 attcctttgc ggaacataag aaaaccactt atattccaca aaagaacggt tttatttaag
    16201 caccaataat gcgattgaat agctctagta aaatgtcttt tactccagca gcgttgaaga
    16261 ctaagccaac cgcaataatc gcaataatta aaaagccaat cagtttgcta aactcacgct
    16321 tgaagccaag atacaagcca atcacaacga ttgctaaaag caccagtgat tgagcgtttg
    16381 atagaaacca gttataaagg ttttgtccaa aattcataaa aatgttctcc tctctatatt
    16441 caatgaattt gtatttgagt tatttttttg gggtatacac tttctggtat ggatagagat
    16501 tccatgccag atttttttat acaaaaaaga ggacatttgc tgtcccctcg ttatacaatc
    16561 aattcaccac aaaaatcatg aaagaaggtt aaacaaatgt cccataatga ttctatccta
    16621 aatattcttg gaattaaaga taaaaatatt aaaattattt ctgttgaaga agctgaacac
    16681 aacaacgatt ctgttaaaga gtatataacg ctaataacag ctactctttc ttatccgatt
    16741 aatcgttgtc gtaactgtgg ctttcccaca gttaataagg atggctttcg caaaactcat
    16801 gtacgactgg caagtttaaa tgggagaaga tatgaactag agcttcgtaa acaacgctat
    16861 aaatgtaaat catgccatac tacttttggt gctattacta atttaaccaa agaaaatcaa
    16921 accttatcca gtgatctcaa aaatcaaatc atgcttttag ctcgtaaagg cttatctggt
    16981 cagcttattg ctgaaatgtg tcactgctct cctagcagtg ttcgtcgaac aatcttagag
    17041 cgcatggaac cacactatcg tgtggctaag ttgcctaagc atctatgttt tgacgagttt
    17101 cgttcaatta agtctgtgat gtcctttatc tgttgtgacg ctgaaaccca ccaaattgtc
    17161 acaaagttac aggatcgtct atcacctacc attgttgatt attttgaaag tcgttattca
    17221 aaagccgaac gcgaatgcgt tcaatcagtt gtaattgatt taaatgctca atatcaaagt
    17281 tttatctatc gccttttccc taatgccaat atcattattg atcgcttcca ccttgtacaa
    17341 ttagctggtc gcgctttgga caattgtcgt atctctatcc taaagcaact tgataaacag
    17401 agccaagaat ataaaattat gaagtcacat tggaagctat tccataaaaa agctgaagat
    17461 cttcaccctg aagaagtagt ttttcttcgc ggcgttaaac aatatatgac tcgccaaaat
    17521 gctgttgatc tcattactag taaattttcc aagttcgctg aagtatacca aacttaccaa
    17581 gatatcacga aagccctaaa cgagcgcaat agtgaattac tagagtcaac catcttagac
    17641 taccaaaaaa ccaatacaga aatggatact gctattcaaa cccttcgtca aaacagaaaa
    17701 tatgtcttaa atagcgctaa atttgaatac tctaatggtc ctttagaagg catcaatcgc
    17761 aaaatcaaaa ccctaaaacg aacttgttat ggttttgcca atcaaaaatt tttcttttta
    17821 agaatcgatt gtattttttc gtaaaaaaat accccctaca ttttcgtagg aagtattttt
    17881 agtcaaccat accagttgac agatatccca cttaggacat tttcctacaa ggggtcccga
    17941 gcgcttagtg ggaatttgta ccccttatcg atacaaattc cccgtaggcg ctagggacct
    18001 ctttagcttc ttggaagctg tcagtagtat atctaataat ttatctccat tccctttagt
    18061 aacgtgtaac tttccaaatt taaaaaagcg actcatagaa ttatttcctc ccgttaaata
    18121 atagataact attaaaaata gacaatactt gctcataagt aatggtactt aaattgttta
    18181 ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc
    18241 gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg
    18301 tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt
    18361 ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg
    18421 ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag
    18481 acgcacggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatat
    18541 tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa
    18601 ttaaagtgac acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt
    18661 ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt
    18721 tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attc
//
LOCUS       DAVHIU010000029        18626 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVHIU010000029 DAVHIU010000000
VERSION     DAVHIU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.031"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(233..1039)
                     /gene="yidA"
                     /locus_tag="VVN06_000874"
     CDS             complement(233..1039)
                     /gene="yidA"
                     /locus_tag="VVN06_000874"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU5562452.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            complement(1052..2356)
                     /locus_tag="VVN06_000875"
     CDS             complement(1052..2356)
                     /locus_tag="VVN06_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5562453.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            2428..2805
                     /locus_tag="VVN06_000876"
     CDS             2428..2805
                     /locus_tag="VVN06_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU5562454.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2896..3228
                     /locus_tag="VVN06_000877"
     CDS             2896..3228
                     /locus_tag="VVN06_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU5562455.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3240..4811
                     /gene="ffh"
                     /locus_tag="VVN06_000878"
     CDS             3240..4811
                     /gene="ffh"
                     /locus_tag="VVN06_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5562456.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPNRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPNLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5006..6289)
                     /locus_tag="VVN06_000879"
     CDS             complement(5006..6289)
                     /locus_tag="VVN06_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU5562457.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6543..7256)
                     /gene="rsmG"
                     /locus_tag="VVN06_000880"
     CDS             complement(6543..7256)
                     /gene="rsmG"
                     /locus_tag="VVN06_000880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5562458.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7350..7910
                     /locus_tag="VVN06_000881"
     CDS             7350..7910
                     /locus_tag="VVN06_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5562459.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7912..8811
                     /gene="htpX"
                     /locus_tag="VVN06_000882"
     CDS             7912..8811
                     /gene="htpX"
                     /locus_tag="VVN06_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU5562460.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRIFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8855..10381)
                     /locus_tag="VVN06_000883"
     CDS             complement(8855..10381)
                     /locus_tag="VVN06_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5562461.1"
                     /translation="MIKINHLTITQNKDLRDLVSDLSMTIQDGEKVAIIGEEGNGKST
                     LLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFFLDSIDLDYSIL
                     YRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLDEPSNDLDLETV
                     DWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLVEHLDYESYSEQ
                     RKAKFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGRLLAKKMKTVLS
                     QEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLSIDDRVLVQKLQ
                     LTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHKKLQLDLSPIAY
                     LSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDLVLRKPNFLLLD
                     EPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTEHGLELVNLEDV
                     "
     gene            11140..11682
                     /locus_tag="VVN06_000884"
     CDS             11140..11682
                     /locus_tag="VVN06_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5562462.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11682..12311
                     /gene="nth"
                     /locus_tag="VVN06_000885"
     CDS             11682..12311
                     /gene="nth"
                     /locus_tag="VVN06_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5562463.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12522..13043
                     /gene="pyrR"
                     /locus_tag="VVN06_000886"
     CDS             12522..13043
                     /gene="pyrR"
                     /locus_tag="VVN06_000886"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5562464.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13062..13985
                     /locus_tag="VVN06_000887"
     CDS             13062..13985
                     /locus_tag="VVN06_000887"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5562465.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14035..15114
                     /gene="carA"
                     /locus_tag="VVN06_000888"
     CDS             14035..15114
                     /gene="carA"
                     /locus_tag="VVN06_000888"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5562466.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15427..18603
                     /gene="carB"
                     /locus_tag="VVN06_000889"
     CDS             15427..18603
                     /gene="carB"
                     /locus_tag="VVN06_000889"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5562467.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5427 a         3597 c         4170 g         5432 t
ORIGIN      
        1 gaagagagta tttaaaatat cttgggaaat tataatttct ccctgttttt tgagtccaat
       61 ctatgagtat gagttgccca ggagtaaggc cgcactctaa tttgtttagg taaaaattgt
      121 tacaatcaga atttgtgtct ctattatgat tattttgttt taaattaaat agtttgctaa
      181 aaaaattcat tggattttat tgctaatcaa ttcttattga aaaatgatac ttttacagta
      241 cccatgtacg gatggcatgg gcaacgccag attcatcatt agatttagtg atgtatttgg
      301 cgattttttt gatttctgga tttccatttt ccatgacaac agggttgcca acgacttcca
      361 gcatggcacg gtcattttct tcgtctccga tagccatggt ttcatctttg gttaatccta
      421 gtttttcagc taagtgagta atagctgaac ccttgtctac attcttttta aggagttcga
      481 ggtagaaagg agcagatttg ttgatagagt agtgctcgta aaattcggct ggtatttttt
      541 caatcgcagc atcgagaatt tctggttcat cgataaacat acatttaaca atttctttgc
      601 cagccatttc ttcaggggta cggtagaaga taggcatgct gacgagggtt gattcgtgta
      661 cagtgtatgt tccgatattg cgatttgcag tatagatacc gtccttggta atggcatgca
      721 tgtggacacc gagcttgcga ctgaggaatt ccatatccag ataatcctca taggtcaagg
      781 attcgctgat aatctcatgg ccagtagcag tttcttggac aagggcacca ttgaaggtca
      841 ccacatagtc accttggtct ctcaactgca agtcgtctag aagtttggca acacctgcga
      901 tggggcggcc agttgcaatc acgactttga caccagcttc tttggcatct tggatggcag
      961 aaaagacttc aggagtgatt tccttttggc tgttgacaag ggttccgtcg atatcaacgg
     1021 caattagttt aatactcata aatttcctct tctagttttt atttggggta aaatgatcgt
     1081 tctcaatcaa gtataaaaat tgctgggtaa tgctagcaaa gatactgttt tgatctaaca
     1141 tttctttcgg aaaatagaaa cgattatctc cgtgacgact accagcaagg gattggacga
     1201 taggagacag gctagagagt tcggccagtt ctccattctt ttgtaaaatc tcaatctgtg
     1261 tccgtggatt ttcagattcg ggacgataga tatcataagg gaggtcaaag ttcttatgaa
     1321 tggcagtata gtagtcggga tcaaagccaa tgtcctcaac caattttctc atgctagcga
     1381 gttggtcttg atcttcttgt gaaaaggtaa tggatttaaa gaccttgcgg ttgacaaagc
     1441 gttgcgataa atctgcgaga attttgtcag gactggtcat ccagagctgg aagtaggtat
     1501 tcatcacacc atcatctaga gccagatagt cagacaaggt cacatttttt tcaaagaaag
     1561 gcaggaggtg tggggaagtt cgtgcaaaga agtctttgtc cttagggtag agttccttag
     1621 cgcgcttgag aagattctgt aggagaactt ccatagcgcg tgttgctggg tggaaataga
     1681 cctgcatata catctggtag cgactgagga catagtcttc gatggcgtgc atgccattgc
     1741 gctgaaaggc gataccattt tcgataggac gaatgactcg gaggattcga gtcaggtcaa
     1801 attccccata ggatgctcct gtaaaatagg agtcacgcaa gagatagtcc atgcgatctg
     1861 cgtcaatctg actagaaatg agttgtacga cctgcttgtt aggataggta tggtctatga
     1921 cactggcaac tttctctgga aaatcgggcg ctacttgtag caagacttgg tgaatctctg
     1981 tctcaggact ttgaataatc tcctgagtaa tagcttcatg gtctgtatca aagagatgtt
     2041 caaaagtatg ggagtaggcg ccatgcccaa ggtcatgtag gagagcagcg atcatggtca
     2101 agagagactc ggcaggattc cattcctcag gatatttttc ttcgaaaatc tctgtgatgc
     2161 gtcgtgcaat ttcatagact cctagacagt gagagaagcg actgtgttct ccaccgtgga
     2221 aggtataact ggaagttccc agttgtttga tccggcgcaa acgctgaaat tcttttgtat
     2281 taatcaagtc atagatgatt tgattattga catggatgta gttgtgaact gggtcacgga
     2341 atactttttc gttcatatga ccattatagc aaaatcctgc tctttttggt aaaatagtag
     2401 gacaagagac aagaaagagg gcctgatgtg aagattcgga tgcgaaatac aattcagttt
     2461 gatgagcagt tggaagtgat tgaccagctt tatgatgtgg aagtgcatga aaaaggagat
     2521 tatagctacc tgcttttcta taatgaggaa aaggaaaaag tagtgattaa atttcatggt
     2581 caagaacttg tgatgagccg attttctaat cccaagacca ttatgcgctt tctaaagaat
     2641 agcgatagtt tagcctatat tcctacacct atgggcatgc aggagtttat catccaaacg
     2701 agccattatc aagttgatag gcaaaagatt gaactagatt atcaactaca aaatcaagag
     2761 ggacatcctt ttgccagcta tcaattggaa attacttggg gctaggctca tgtctttttc
     2821 aaatttagct atctatcttc ttggatcggc ttcctagtgt ggtagattag gctaattttt
     2881 ggtataataa aagttatgga aatcgaaaaa accaatcgta tgaatgcgct ctttgaattt
     2941 tatgcggcgc ttttgacaga taagcaaatg aattatatcg agctctacta cgctgatgat
     3001 tacagccttg ctgaaattgc cgaggagttc ggtgtcagtc gtcaggctgt ctatgacaat
     3061 atcaagcgaa cagaaaagat tctggaagat tatgagatga aattgcacat gtactcggac
     3121 tatattgtcc gcagtcagat ttttgatcag attttggagc gctatcccaa ggataacttt
     3181 ctgcaggagc agatagaaat tttaacaagc attgataata gagaataaga ggaagaaaaa
     3241 tggcatttga aagtttaaca gaacgtttgc agaacgtctt taaaaatcta cgtaaaaaag
     3301 gaaaaatctc tgaatctgat gtccaagagg caaccaaaga aattcgcttg gccttgctcg
     3361 aggccgacgt tgccttgcct gttgtaaagg actttatcaa gaaagttcgt gagcgtgcag
     3421 tcgggcatga ggtcattgat acacttaatc ctgcgcaaca gattattaaa atcgttgatg
     3481 aggaactgac agccgtttta ggttctgata cggcagaaat tatcaagtca cctaagattc
     3541 caaccatcat catgatggtt ggtttacaag gggctggtaa aacaaccttt gctggtaaat
     3601 tggccaacaa actcaagaaa gaagaaaatg ctcgtccttt gatgattgcg gcggatattt
     3661 atcgtccagc tgccattgac cagcttaaga ccttgggaca acagattgat gtgcctgtct
     3721 ttgcacttgg aacagaagta ccagctgttg agattgtacg tcaaggtttg gagcaagccc
     3781 aaactaatca taacgactat gtcttgattg atactgcggg tcgtttgcag attgatgagc
     3841 tcctcatgaa tgagcttcgt gatgtgaaag cattggctca accaaatgaa atcttgcttg
     3901 tcgttgatgc tatgattggt caggaagcag ccaatgttgc gcgtgagttt aatgctcagt
     3961 tggaagtgac tggggtcatc cttaccaaga ttgatggcga tactcgtggt ggtgcggctc
     4021 tatcggttcg tcacatcact ggaaaaccaa tcaagttcac tggtacaggt gaaaaaatta
     4081 cagatatcga aaccttccac ccagaccgta tgtctagccg tatccttggc atgggggata
     4141 tgctcacttt gattgagaaa gcttctcagg aatacgatga acaaaaagcc cttgaaatgg
     4201 ctgagaagat gcgcgaaaac acctttgatt ttaatgattt catcgatcaa ttagatcagg
     4261 tgcaaaatat ggggccgatg gaagacttgc tcaagatgat tccaggtatg gccaacaatc
     4321 cagcacttca aaacatgaag gtggatgaac gccagattgc tcgtaaacgt gccattgtgt
     4381 cttcgatgac acctgaagaa cgtgaaaacc cagatttgtt aaatccaaac cgtcgccgtc
     4441 gtattgctgc tggttctgga aatacattcg tcgaagtcaa taaattcatc aaggacttta
     4501 accaggctaa acagctcatg cagggtgtta tgtctgggga tatgaataaa atgatgaagc
     4561 aaatggggat taatccaaat aaccttccta aaaatatgcc aaatatggga ggaatggata
     4621 tgtctgccct tgaaggaatg atgggacaag gcggtatgcc taacttatca gctctcggag
     4681 gagcaggaat gccagatatg agccagatgt ttggtggcgg tttgaaaggt aaaattggtg
     4741 aatttgccat gaaacagtcc atgaaacgta tggctaacaa aatgaagaaa gcgaagaaga
     4801 aacgcaagta agacaagaga ttgatgcaaa tctcatttga tgagaaaata aagtaaaaat
     4861 ttctatgata aaacgcacag tatcaaggtt ttagaatatc tgatattata cgttttttga
     4921 ttttaataat tttgttaaga cacttttatt atggtatacg aaaaatccac tcagctgtct
     4981 gagtggatta ggtgtattta gactcttagt ctttattttc gtatggcaag atcaagttca
     5041 agatgattcc tgtcatggct gaaagggcag tacctgaaag tgtaactgga ccaagtttaa
     5101 ggatagctcc tccaagtcca agaaccaaca tagcacttgc gatgatgagg tttcgcattt
     5161 gagcgaaatc aacacgttct ttaatcaaga ctttcaaacc attgctggcg ataaccccat
     5221 agagaaggat tgacatacca ccaagtacag cgtttggaat agttgaaatc aaggcagtga
     5281 atttaccaag gaagctgagg gcaatcgcga tgaaggcagc gttacggata actgagacag
     5341 aagcgatacg agtcataccg ataacccctg tattttctcc gtaagttgta ttggctggtc
     5401 caccaaggaa ggcagaaaca gaagttgcga taccgtcacc aataagagta cggtgaagac
     5461 ctggttcttt taagaattga cggccacaga tttgacccaa aacagtatgg tctccgatat
     5521 gttcagaaat tgttacgata gcgattggca agatagcgat ggcttctgga ccaaagtaaa
     5581 gattgtactc tttaaaggca ccacctgtgc taaatggcaa gtagaaacca ggaatttcaa
     5641 accagttagc ttctaggaca ggcgtaaagt caaccaaacc aagagttagt gcgaagaggt
     5701 aaccaccgat aatggcaaag aggaatggaa tgattcgtag gaagcctttt ccttttgtat
     5761 tgataaaggc agcaattagg aaagtaacaa cggctaccag agcatttttc caatttccgt
     5821 ctgctacaag acctgcattg gtaacagctg aacctgcaag tccaagaccg ataacgatga
     5881 tcataggacc gataatgatt ggtggcaaga gtttatcaat ccattttgtt cctacaaatc
     5941 ggatgctggt agcaacaagg acatagacca aaccagtcaa gataacccct gtttgggcag
     6001 cagatacatc cccccccatt tctttcatag ccagtgacat agctgtgata aaggcgaatg
     6061 aagaacctag ataaactgga actttaaaac cagtagcaat catgtagatg agtgttccaa
     6121 cacctgaagc aaaaagggca acagatacag gcattcccaa aatcaatggt accaagatgg
     6181 tcgcaccaaa catggcgaaa acgtgttgga aactaaggag aattcctttt ccagccgaag
     6241 gacgttggtc aacgtctagt aacaaatcaa cagttgattc ttgtttcata taaacctcac
     6301 ttttgggcat caaaaaagag cccattattt tacagcaata ggccctcaga gtaagacttc
     6361 tttaaaattt agattttaaa gagttttaaa tatttctgcg tcttcgtcac ctcacgggat
     6421 gacattaaaa acctttgctt aggatagtat agcagaaaaa aatgattttg taaatcattt
     6481 tttcccgagc ctagaaataa aagagcgagg ctgattttgt aaacctttgt ttactaaaaa
     6541 atttaaagtg ggcgtttatt tggcatacca gccttacgtg gatatttatt tggtgtttct
     6601 tttttctttt ctaccactgt gatatagcgc ggatctctat tcggtagggc gtagctgaga
     6661 ttgtcttcga ccttactaaa aaggagattg agggcattct tagcttctaa taattcctca
     6721 ggcgcattgc tagccttgag tgctaatagt ttgccaccaa ccttaaggta gggaatagtc
     6781 aattcagata ggacctgcat acgggcaacc gcacgagctg ttacaaaatc atattgagca
     6841 cggaagttct tgtcttgggc aaaatcttcg gcacgtccgt ggtagaaatg aactccgttc
     6901 aaatccagtt cttgagccaa gagttgtagg aagttgatgc gcttattgag tgaatcaata
     6961 atggtcacat ctaactccgg atagagaatt ttcattggta gactcggaaa tcctgcccca
     7021 gccccgatat caagaagttt gatagtttca ttgggaatca aaccttgaag aatgggtgca
     7081 atcgaatcgt aaaaatgttt gagataaact tcttccttgt ccgtaatcgc cgtcaaatta
     7141 atcttctcat tccactcgac caagagctca aaataacgtt caaattgttc tttttgctgg
     7201 tccgaaagtg gaagattctg ctcggcaagc aagttgtaaa atgtttttgg tttcatagtt
     7261 atttccttct ttagtatcat cttattttac cacaatcata gaaaatttga tatactggta
     7321 ctaatctaaa agcagaaagg acttatatta tgacttggat tattcttgga gttttagctc
     7381 ttgttgttat ttttgtgatt gttagctata acggtttggt taaaaatcgt atgcaaacca
     7441 aggaggcttg gagtcagatt gatgttcagt tgaaacgtcg caatgatctc ttgccaaact
     7501 tgattgagac tgtaaaaggt tatgccaaat atgaaggttc tacccttgaa aaggtggcag
     7561 aactacgtaa ccaagtggcg gcagcgactt caccagcaga agctatgaaa gccagtgatg
     7621 ccctcactcg tcaggtttca ggtatttttg cagttgcaga aagctatcca gatttgaaag
     7681 ctagtgctaa ctttgttaaa ttgcaagagg agttgacaaa cacagaaaat aaaatttctt
     7741 actctcgtca actctataac agtgttgtca gcaactacaa tgtaaaatta gaaactttcc
     7801 cgagcaatat tatcgctgga atgtttggat ttaaagcggc agatttcctt caaacacctg
     7861 aagaggaaaa gtcggttcct aaagttgatt ttagcggttt aggtgactaa gatgttgttt
     7921 gatcaaattg caagcaataa acgaaaaacc tggattttgt tgctggtatt tttcctactc
     7981 ttagctcttg ttggttatgc ggttggttat ctctttataa gatctggact tggtggtttg
     8041 gttatcgcac tgattatcgg ctttatctac gctttgtcta tgatttttca atcgacagag
     8101 attgtcatgt ccatgaatgg agcgcgtgag gtggatgagc aaacggcacc agacctctac
     8161 catgtagtgg aagatatggc tctggtcgct cagattccta tgccccgtat tttcattatt
     8221 gatgatccag ccttaaatgc ctttgcgaca ggttctaatc ctcaaaatgc ggctgttgct
     8281 gcgacttcag gtctactagc tatcatgaat cgtgaagaac tagaagctgt tatgggacat
     8341 gaagtcagtc atattcgtaa ttatgatatc cgtatttcga ctattgcagt tgcccttgct
     8401 agtgctatca ccatgctttc tagtatggca ggtcgtatga tgtggtgggg tggagcaggt
     8461 cgcagacgaa gtgatgatga ccgagatgga aatggtcttg aaatcattat gctagtggtt
     8521 tccctactag ctattgtact ggcacctctc gctgcaacct tggttcagct cgctatttct
     8581 cgtcagaggg aatttctggc agatgcatct agtgtcgagc tgactcgcaa tccccaggga
     8641 atgattaatg ccctagataa gttggacaat agcaaaccta tgagtcgcca cgtcgatgat
     8701 gctagcagtg ctctttatat caatgatccc aagaaaggtg gggggttcca aaaactcttt
     8761 tatacccacc cacctatctc agaacggatt gaacgtttaa aacagatgta aaagaatcca
     8821 gaggtcgtct ctggattttt gctatgttgc aaatttatac atcttctaaa ttaactaact
     8881 ccaaaccgtg ttctgtcaag cggtagatgc ttgaacagac ctcttttaag aaacgacggt
     8941 catgcgaaac agtgatgaga ccgcctggat aggttgcaaa gagttttctg atttggggtt
     9001 gagaagtggg agaaaagttt cgtgtgggtt catccagcag gagaaagttt ggtttgcgca
     9061 ggactaaatc caaaagcagg agttttccct gttgtccgcc agataaggag cgaatttgat
     9121 gctgcatttc tggataactg aaattgagac tagctaggtg agattggatt ttctgtagtt
     9181 cctctttttc cccagttttg ctgaggtagg ctattggtga taaatccaat tgcagttttt
     9241 tgtggtaatc ttgtggcata aaaccaaggg aaatctctct tttatcattc agaagttgct
     9301 ttaatttggc taacagagtt gatttcccaa caccatttgg cccgataata ccgatttttt
     9361 cttgcccacg gacagttagt tgtagttttt gaaccaaaac tcggtcgtca atggacaaat
     9421 tttctttttc cagttggact aagactttag aagctggtaa tggttggatg tctgaaaaga
     9481 aaagttggat ttgttcctct tcaagtggct tttgagtcat ggactgagct gccttttcgt
     9541 agcgtttttc ttgtgagagg acagttttca tctttttagc caataggcga ccggcagtac
     9601 tgtctttggt agctcgaagc gcagtttcta cattttgttt gactctccga tgtttttcca
     9661 tggttttatc gtaggctctt tggttgttag cagcttgctg actttgtttg gcaaatttag
     9721 cctttctctg ctcactatag ctttcatagt ctaaatgctc tactagcgtt tccgcttctt
     9781 tacggtgttt gaccagtcgc aagtgaacaa tagtgtctgc cgtttcagaa agaaagtctt
     9841 catcatggga aatgaaaata acggtttgcc tggtcttttg aatctggcct tttaaccaat
     9901 caactgtctc aaggtctagg tcatttgaag gttcatctaa aaatagaatc tcaaagggtt
     9961 tggctaactc atggataagc tgaattttca aagcttctcc ccctgataga ttgccaatct
    10021 cttggtcact tgcgaaacga ttgctatcaa aatgcaattc ctccgccaaa cgatagagga
    10081 tactgtagtc taaatcaata gaatctaaaa agaagtagtc gtgtaaagtt ttctttttca
    10141 gctcctcggg aagtttttga ggaatgtagg ccagtgactg ataatcagac tggatgtctc
    10201 ccttgatagt gaaatcagac aaatcttccc ccattaaagt tttaagtaag gttgatttac
    10261 catttccttc ttcgccaata atagctacct tttccccgtc ttgtatggtc atgcttaggt
    10321 cagagacaag gtctcgtaaa tctttgtttt gtgtgatggt caggtgattg atttttatca
    10381 taactattgc cctttctaga atgttcatag tgcataacaa aaagctctac tttacctagt
    10441 agagcccaaa gtctatgtca aaaaaactct attaacatgc cagaaaaagg gcataaaaga
    10501 gcacacaaaa agagacaaaa acaagatcta ctaaataaag gttctttatt tgatagactt
    10561 agttgttcat gtctccctta cctcctagta ttagtatctt tatactatac ttttaaggaa
    10621 attttgtcaa cagaaaactg gaaattctat gctctaaaat ccaaaaacag gtctatagag
    10681 acttagtacg tgtttgacat gctcgtaatg gattttatca tagtttcgcc aagcagtgcg
    10741 ttcttgattg tttagtttta ggctacccag tccaatcata ggactggtgg gttggtaaaa
    10801 tttctcaaga tcaattaaga tactgttgtt caacttttcc gttttttgag ttcttgcata
    10861 gtagtgttta gcagctcttg tagtcgaata tcatctgccg taccctgttt acagttttgg
    10921 tagcttttat ttaacttgga taggagttga atgagtttct tgtcttccgt gtgagcatcc
    10981 tccttgatct gatgtactct ttccctatag tatagcacta aaaaataaaa ctgtcatagt
    11041 gaatatgtta aaatttcgaa ttttaattac aaagtttggt gtgctgattt gtagcctttt
    11101 catggtataa tcaagagagt aaatctttat ggaggaagta tgaaattaaa tattcaagaa
    11161 attcgtaagc agtctgaagg tttgaacttt gaacaaacgt tagatttagt tgatgacctg
    11221 cgtgcacgta atcaagaaat tttagatgta aaagatatcc ttgcagttgg gaaagtacaa
    11281 tatgaagacc gtatgtattt cttagattat caactatctt ataccattgt tcttgcttcg
    11341 agtcgcagta tggagccagt tgagttagtt gaatcttatc cagtcacgga agttttcatg
    11401 gaaggcgcaa ctaaccagct agatcaagaa gttttagatg atgacttggt cttgcccatc
    11461 gaaaatgggg agcttgacct tgctgagagt gtatcagaca atatcctgct aaacattcct
    11521 atcaaggtct tgacggctga agaagaagct ggtcaaggat ttgtctcagg aaatgactgg
    11581 caaatcatga cagaggaaga ataccaagct caacaagcag taaagaaaga agaaaacagt
    11641 ccttttgctg gcttacaagg actatttgac ggagatgaat aatggtcttg tcaaaaaaac
    11701 gagcacgaaa ggtgctagaa gaaatcattg ccctcttccc agatgccaag cctagtcttg
    11761 attttaccaa tcattttgaa ctcctggttg cggtcatgtt atcagcccag acgacagatg
    11821 cagcggtaaa taaggccaca ccaggtctct ttgttgcctt tccaacaccc caagctatgt
    11881 ctgtagcgac agagagtgag attgcttcac atatttctcg cttgggattg tatcggaata
    11941 aagctaaatt ccttaaaaaa tgtgcccaac agttactaga cgattttgat ggtcaagtcc
    12001 ctcagacacg tgaagaattg gagagtttgg caggtgttgg tcgcaagaca gccaatgttg
    12061 tcatgagtgt aggatttggg attccagcct ttgcagtgga tactcatgtg gagcgtattt
    12121 gcaaacacca cgatattgtc aaaaaatcag cgacgccact tgaggtggaa aagcgggtca
    12181 tggatatctt gccgcctgag cagtggttag ctgcccatca ggccatgatt tattttggaa
    12241 gagccatttg ccatccaaaa aatccagagt gtgaccagta cccacaatta tatgatttta
    12301 gcaatttgta agatgctaca ttgaaacttt ttgcttgatt ttaagcatgc tttgtgctat
    12361 agtaaatgat aactaaatat tcctttaaac ctgtcccgtg aggcaggcaa ggagcgcgat
    12421 aaagtagaag ggtgaagtct atactgtgtg cagtagggct cgcttggcta taaatttcaa
    12481 gtctccttgt ttaaggagac ttttcttttt ggaggtttgt catgaaaaca aaagaagttg
    12541 tagacgaatt gaccgtcaaa cgagcgatta cgcgtattac ttatgagatt atcgaacgca
    12601 acaaagattt gaataagatt gtcttggctg gtattaaaac tcgtggtgtc tttattgccc
    12661 accgaatcca agaacgtttg aagcagttag aaaatctttc agttcctgtt gtggaattgg
    12721 atactaaacc tttccgtgat gatgttaaaa gtggagaaga tacttctttg gtttctgtcg
    12781 atgtgacaga ccgcgaagtt atcttggtgg atgatgtgct ctatacaggt cgtaccatcc
    12841 gtgctgctat tgataatatt gtcggtcatg gtcgtcctgc gcgtgtgagt ttagcagttc
    12901 tagtcgatcg tggacataga gaattgccaa tccgtccaga ttacgttgga aaaaatatcc
    12961 caaccagtcg ttctgaagaa atcatcgtag agatgacaga acttgatgac caagacagag
    13021 ttctgattac tgaagaagct tagaaagtta aaggagtagc catgtcagaa aatcaacaag
    13081 cattgaacca tgtggtgtcc atggaagacc tcactgtcga tcaagtaatg aaattgatca
    13141 agcgaggaat tgagtttaaa aatggagccc agcttcccta tgaagaccat ccgattgttt
    13201 ccaatctctt ctttgaggat tctacacgga cacataagtc ctttgaagtc gcagagatta
    13261 aacttggatt ggaacgactt gactttgatg tgaagactag ttcggttaat aagggtgaga
    13321 cactttatga caccattttg actctgtctg ctttaggagt ggatgtctgt gtgattcgcc
    13381 atccagaggt cgactactat agagaattga ttgctagtcc aacgattacg acttccatca
    13441 tcaatggtgg agatggttcg ggccaacacc ctagccagag cttgcttgat ttgatgacca
    13501 tttatgagga atttggccac tttgagggtc ttaaagttgc tattgcaggt gacttggacc
    13561 actcacgcgt tgccaaatcc aatatgcaga ttttgaaacg cttgggatct gaactctttt
    13621 tcgctggacc tgaggaatgg agaagtcaag agtttgcaga ctatggacag tttgtaacca
    13681 ttgatgaaat cattgatcag gttgatgtca tgatgtttct ccgtgtgcaa cacgaacgcc
    13741 atgatagtgg agctgtattt tcaaaagaag actaccatgc ccaacatggc ttgactcaag
    13801 aacgttacga tcgtttgaaa gaaacagcaa tcctcatgca cccagctcca atcaatcgtg
    13861 atgtagaaat agcagaccac ttggttgaag caccaaaatc acggattgtc caacaaatga
    13921 ccaatggtgt ctttgttcga atggcaatct tagaatccgt actagcgagt agaaacgcca
    13981 actaaaacac aagacgttaa aagacgccag tgagcgtcca cgagaggtga aaatatgaca
    14041 aaaagaattc tagtattaga agatggcaca gtttttgaag gcaaggcctt cggagcagat
    14101 attgatgtaa caggcgaaat cgtctttaat acagggatga ccggctacca agaatccatt
    14161 acagaccagt cttataatgg acaaatcttg acctttactt atcctttggt aggaaattat
    14221 ggtattaatc gtgatgatta tgaatccatt attccaactt gtaagggagt cgttgttttt
    14281 gaagaagcgc gtagagctag caactggcgc aaccaaatga ccttggatga atttttgaaa
    14341 gccaagaaaa ttccagggat ttcaggaatt gatacgcgtg ctcttaccaa gattatccgt
    14401 aagcatggta ctatgcgtgc aaccttgacc catgttgggg acagtatgga ccatgtgacg
    14461 gaccagctcc aagcaacagt tttaccgaca gacaatatca aacaggtttc tactaaaacg
    14521 tcttatccag ctccaggagt tggtttgagc gtggtgctag tggactttgg tctcaagcac
    14581 tcaatcctac gtgaactttc taaacgcaac tgtaacgtga cagttgttcc ttattcgaca
    14641 acggctgaag aaattctcca tctcaatcct gacggagtta tgttgtcaaa tggtccaggt
    14701 aacccagaag acgttccaca agcactcgac atgattcgcg gtgtacaagg aaaaattcca
    14761 atctttggta tttgtatggg acaccaactc tttgcaatgg caaacggggc taagacctat
    14821 aagatgaagt ttggccaccg tggattcaac catgcggtac gtgaaatcgc aacaggacga
    14881 gtagacttca ccagccagaa ccatggttat gcagtcagcc gcgaggattt gccagagcac
    14941 ttgattatta cccacgaaga aatcaatgac aagtcagtgg aaggtgtgcg ccacagatac
    15001 caacctgctt tctctgttca ataccaccca gatgcagctc ctgggccaca cgacgctagc
    15061 tacctatttg acgagtttat cgagatgatg gaagttttta aacaatcaaa ctaagaacga
    15121 gtaaaagctc gtcggagaat ttatgtaact gaacacggac ggaaagctca gaatgtagag
    15181 aaaccaactt ggattgtcaa tccttccttg atttcctaaa attcaatcgc tttctattcg
    15241 tcctttgtat cttatttaat gtcgccctga gaggcgacga atagaaagga gaagggtgct
    15301 ccgtatttgc tgaactgaat acgggctacg gacggtgcta aaaagatagt tattcctaga
    15361 actgacagtt cttcgtcata actcctattt tagctttgtc cgcttgacac ccttaatatc
    15421 ttatatatgc ctaaacgtac tgatattcaa aaaattatgg tgattggttc tggtccgatt
    15481 attattggtc aggctgctga gtttgactac gctgggactc aggcttgctt gtcgttgaaa
    15541 gaggaaggtt atgaggttgt tttggttaac tcaaatcctg caaccatcat gacggacaag
    15601 gaaattgctg acaaggttta cattgaaccg attacacttg agtttgtgac acgtattctt
    15661 cgtaaggaag gtccagatgc cttgctacca acactcggtg gtcagacagg tctcaacatg
    15721 gccatggaat tatctaaaaa tggtatccta gacgagcttg gcgttgaact tctgggtacc
    15781 aaattatctg ccattgacca agcggaggac cgtgacctct ttaaacaatt gatggaagag
    15841 cttgaacaac ccattccaga atctgaaatt gttaacacag tagaagaagc agttgccttt
    15901 gcagcgacaa ttggctaccc agtcatcgtt cgtccagcct ttacccttgg tggtactggt
    15961 ggtggtatgt gtgccaacga gaaagaattg cgtgaaatca ctgaaaatgg tttgaaattg
    16021 tcacctgtta cccaatgttt gattgagcgt tcgattgccg gtttcaagga aattgaatac
    16081 gaagtgatgc gtgactcagc tgacaatgcc ttggttgttt gtaacatgga aaactttgac
    16141 ccagttggga ttcacacagg ggattccatt gtatttgccc ctgcgcaaac catgtcagac
    16201 tatgaaaacc aaatgcttcg tgatgcgagc ttgagcatta ttcgtgccct caagattgaa
    16261 ggtggatgta atgttcagct agctcttgat cctaacagct tcaagtatta tgttatcgaa
    16321 gtaaaccctc gtgtatcacg ttcgtcagcc cttgcttcta aagcgacagg atatccgatt
    16381 gctaagttgg ctgccaagat tgcagtaggt ttgaccttgg atgaggttat aaacccagtt
    16441 acaggttcaa cctatgccat gtttgaacca gcccttgact acgtagttgc taagattcca
    16501 cgtttcccat ttgacaagtt tgaaaagggt gaacgccgtc tcggtacaca aatgaaggcg
    16561 actggagaag tcatggcaat cggtcgtaac atcgaggaat ctcttctcaa ggcctgccgc
    16621 tcccttgaaa ttggggtgca ccacaatgaa atacctgaac ttgcagcagt ttctgatgat
    16681 gccttgattg aaaaggttgt caaagcccaa gatgaccgtc tcttctacgt atcagaagcc
    16741 attcgtcgtg gctacacacc tgaagaaatt gcagagctta caaaaattga tatcttctat
    16801 cttgataaac tcttgcatat ctttgaaatt gagcaagaat tgggtgctca tccacaagat
    16861 ctagaagttt tgaaaacagc caaacttaat ggtttttcag accgtaagat tgctgaactc
    16921 tggggaacga ctgatgataa agttcgccaa cttcgcttgg aaaacaagat tgttccagtt
    16981 tataagatgg tagacacttg tgcggcagag ttcgactccg aaacgccata tttctattca
    17041 acctatggtt gggaaaatga atctatcaga tctgataaag aatctgtact tgtcctaggt
    17101 tcaggtccaa tccgtatcgg tcaaggggtt gagtttgact acgcaaccgt tcactctgtt
    17161 aaggctatcc aggcagctgg ttatgaagct atcatcatga actcaaaccc agagaccgtt
    17221 tcaacagact tctctgtatc agataagctt tactttgagc cattgacatt cgaagatgtt
    17281 atgaacgtca ttgacttgga acaaccaaag ggagttatcg ttcagttcgg tggtcaaaca
    17341 gccatcaacc ttgcggagcc attggcaaaa gcaggtgtga ccatccttgg tacacaggtt
    17401 gctgacctag accgtgccga agaccgtgac ctcttcgagc aagctctcaa agaattggat
    17461 attccacagc caccaggaca aacggctacc aatgaagaag aagcagctct tgcagctcgt
    17521 aagattggct tcccagttct tgtccgccca tcttatgtac ttggtggacg tgccatggaa
    17581 atcgttgaaa acgaagaaga ccttcgttct tacatgcgaa ctgctgttaa ggctagtcca
    17641 gaccatccag ttcttgtcga ctcttatatc gttgggcaag agtgcgaagt tgatgccatt
    17701 tcagatggta aaaatgtact cattcctggt atcatggagc atatcgaacg tgctggtgtc
    17761 cactcaggtg actcaatggc tgtttaccca ccacaaacct tgtcgcaaaa ggttcaggag
    17821 accatcgcag actacaccaa acgcttagca atcggtcttc actgccttgg aatgatgaac
    17881 atccagtttg ttatcaagga tgaaaaagtc tacgttattg aggtcaatcc acgtgccagc
    17941 cgtaccgttc cattcctatc taaggtaacc aatattccta tggctcaagt agcgaccaag
    18001 ctcattcttg gtcaaagttt atcagaactt ggctaccaaa atggacttta ccctgaaagc
    18061 acccgtgttc atatcaaggc acctgtcttc tcctttacca aactagctaa ggtagacagc
    18121 ttgctcggtc ctgaaatgaa gtcaacaggt gaagttatgg gttctgatgc tactttggaa
    18181 aaagctctct ataaagcctt tgaagcttct tatctacact tgccaacttt tggtaatgtc
    18241 gtctttacca tcgctgatga tgccaaagaa gaagccttga acttggctcg tcgtttccaa
    18301 aacattggtt atggaatcct tgcgacagaa gggacagcag ccttctttgc tagtcatggc
    18361 ttgcaagccc aacctgttgg taagattggt gatgacgata aggatatccc aagttttgta
    18421 cgcaaaggaa gaattcaagc tatcattaac acagtaggaa caaaacgaac tgctgacgaa
    18481 gatggtgagc aaattcgtcg ttcagccatt gaacacgggg tgcccctctt cacagcgcta
    18541 gatacagcta atgccatgct taaggtactt gaaagtcgta gctttgttac agaagcgatt
    18601 taaatagaaa aataagatat agcaga
//
LOCUS       DAVHIU010000030        18566 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVHIU010000030 DAVHIU010000000
VERSION     DAVHIU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.032"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            27..1217
                     /locus_tag="VVN06_000890"
     CDS             27..1217
                     /locus_tag="VVN06_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU5562468.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     SLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            1366..1737
                     /gene="rnpA"
                     /locus_tag="VVN06_000891"
     CDS             1366..1737
                     /gene="rnpA"
                     /locus_tag="VVN06_000891"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5562469.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            1712..2536
                     /locus_tag="VVN06_000892"
     CDS             1712..2536
                     /locus_tag="VVN06_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5562470.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            2555..3541
                     /locus_tag="VVN06_000893"
     CDS             2555..3541
                     /locus_tag="VVN06_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU5562471.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRNHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            3592..4215
                     /locus_tag="VVN06_000894"
     CDS             3592..4215
                     /locus_tag="VVN06_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU5562472.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYQVTRG"
     gene            4344..5801
                     /locus_tag="VVN06_000895"
     CDS             4344..5801
                     /locus_tag="VVN06_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5562473.1"
                     /translation="MEVVSNVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLIIIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            5824..6105
                     /locus_tag="VVN06_000896"
     CDS             5824..6105
                     /locus_tag="VVN06_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5562474.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            6181..6666
                     /locus_tag="VVN06_000897"
     CDS             6181..6666
                     /locus_tag="VVN06_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5562475.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            6683..7348
                     /locus_tag="VVN06_000898"
     CDS             6683..7348
                     /locus_tag="VVN06_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU5562476.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            7352..8215
                     /locus_tag="VVN06_000899"
     CDS             7352..8215
                     /locus_tag="VVN06_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU5562477.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYGTGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            8217..8921
                     /locus_tag="VVN06_000900"
     CDS             8217..8921
                     /locus_tag="VVN06_000900"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU5562478.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            9104..10777
                     /locus_tag="VVN06_000901"
     CDS             9104..10777
                     /locus_tag="VVN06_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5562479.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            10889..11980
                     /gene="ulaG"
                     /locus_tag="VVN06_000902"
     CDS             10889..11980
                     /gene="ulaG"
                     /locus_tag="VVN06_000902"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU5562480.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            12094..14070
                     /gene="tkt"
                     /locus_tag="VVN06_000903"
     CDS             12094..14070
                     /gene="tkt"
                     /locus_tag="VVN06_000903"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5562481.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            14187..14486
                     /gene="yajC"
                     /locus_tag="VVN06_000904"
     CDS             14187..14486
                     /gene="yajC"
                     /locus_tag="VVN06_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5562482.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            14532..14960
                     /locus_tag="VVN06_000905"
     CDS             14532..14960
                     /locus_tag="VVN06_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU5562483.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            14962..15372
                     /locus_tag="VVN06_000906"
     CDS             14962..15372
                     /locus_tag="VVN06_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562484.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            15672..18323
                     /gene="adhE"
                     /locus_tag="VVN06_000907"
                     /gene_synonym="adhC"
     CDS             15672..18323
                     /gene="adhE"
                     /locus_tag="VVN06_000907"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU5562485.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         5827 a         3335 c         4036 g         5368 t
ORIGIN      
        1 aaacgcttaa aaaggggtat catgttatga caaaaacaat tgcaatcaat gcaggaagtt
       61 caagtttgaa atggcaatta tacttaatgc cagaagaaaa agtattggcg aaaggtttga
      121 ttgaacgtat cggtttgaaa gattcaattt caactgtaaa atttgacggc cgttctgaac
      181 aacaaatttt ggatattgaa aatcatacac aagccgttaa aattttattg gatgacttga
      241 ttcgtttcga tattatcaag gcttatgacg agattacagg tgttggacat cgtgttgttg
      301 ctggtggaga atatttcaaa gaatcaacag ttgttgaggg agatgtttta gaaaaagttg
      361 aagagttgag tttgttggct cctctacaca acccggccaa tgcagcaggt gttcgtgcct
      421 tcaaggaatt gttgccagac attaccagtg tagttgtttt tgatacttcc ttccacacaa
      481 gtatgccaga gaaagcttat cgctactctc taccaacaaa atattacaca gaaaacaagg
      541 ttcgtaaata cggtgctcat ggtacaagtc accagtttgt agcaggagaa gctgcaaaac
      601 tcttgggacg tccattagaa gacttgaagt taattacctg tcatattggt aacggaggct
      661 caattacagc tgtgaaagcc ggcaaatctg tagacacttc tatggggttc actcctcttg
      721 gtggtattat gatgggaacg cgtacagggg atattgatcc agctatcatt ccttatttaa
      781 tgcaatatac agaggatttt aacacaccag aagatatcag tcgtgttctt aaccgtgaat
      841 caggtctttt gggagtttct gctaattcta gcgatatgcg cgatatagaa gcagctgtag
      901 cagaagggaa tcacgaggct agcttggctt atgaaatgta tgttgaccgt atccaaaaac
      961 atatcggtca gtaccttgca gtgctaaatg gagcagatgc cattgttttc acagcaggtg
     1021 tcggtgaaaa tgcagagagt ttccgtcgtg atgtaatctc agggatttcg tggtttggtt
     1081 gtgatgttga tgatgaaaag aatgtctttg gcgttacagg agacatctca acagaggcag
     1141 ctaaaatccg tgtcttggtt attccaacag atgaagaatt agtcattgcc cgtgacgttg
     1201 aacgcttgaa aaaataagtg aaactaaaaa aatattcaat acaaggagtt gggaaagtta
     1261 tttttccagc ttctttttct gatgaaattg tccaaaacct tgctatgatt ggcttttttg
     1321 aaaaatatgg tataatagta gtaatttaat agatggagtt gagttttgaa gaaaaacttt
     1381 cgtgtaaaaa gagagaaaga ttttaaggcg attttcaagg aggggacaag ttttgctaat
     1441 cgcaaatttg tggtctacca attagaaaac cagaaaaacc attttcgagt aggtctatca
     1501 gttagcaaaa aactggggaa tgccgtcact agaaatcaaa ttaagcgacg gattcggcat
     1561 attatccaga atgcaaaagg gagtctggta gaagatgtcg actttgttgt cattgctcga
     1621 aaaggagtcg aaaccttggg atacgcagag atggagaaaa atctactcca tgtattaaaa
     1681 ttatcaaaga tttaccggga aggaaatggg agtgaaaaag aaactaaagt tgactagttt
     1741 gctaggactg tctctgttaa tcatgacagc ctgtgcgact aatggggtaa ctagcgatat
     1801 tacagccgaa tcggctgatt tttggagtaa attggtttac ttctttgcgg aaatcattcg
     1861 ctttttatcg tttgatatta gtatcggagt ggggattatt ctctttacgg tcttgattcg
     1921 tacagtcctc ttgccagtct ttcaggtgca aatggtggct tctaggaaaa tgcaggaagc
     1981 tcagccacgc attaaggcgc ttcgagaaca atatccaggt cgagatatgg aaagcagaac
     2041 caaactagag caggaaatgc gtaaagtatt taaagaaatg ggtgtcagac agtcagactc
     2101 tctttggccg attttgattc agatgccggt tattttggcc ttgttccaag ccctatcaag
     2161 agttgacttt ttaaagacag gtcatttctt atggattaac cttggtagtg tggatacaac
     2221 ccttgttctt ccgattttag cagcagtatt caccttttta agtacttggt tgtccaacaa
     2281 agctttgtct gagcgaaatg gcgctacgac tgcgatgatg tatgggattc cagtcttgat
     2341 ttttatcttt gcagtttatg cgccaggtgg agtcgcccta tactggacag tgtctaatgc
     2401 ttatcaagtc ttgcaaacct atttcttgaa taatccattc aagattatcg cagagcgcga
     2461 ggccgtagta caggcacaaa aagatttgga aaatagaaaa agaaaagcca agaaaaaggc
     2521 tcagaaaacg aaataaataa ggaggaatct ggtaatggta gtatttacag gttcaactgt
     2581 tgaagaagca atccagaaag gattgaaaga attagatatt ccaagaatga aggctcatat
     2641 caaagtcatt tctagggaga aaaaaggctt tcttggtcta tttggtaaaa aaccagccca
     2701 agtggatatt gaagcgatta gtgaaacgac tgttgtcaaa gcaaatcaac aggtagtaaa
     2761 aggcgttccg aaaaaaatca atgatttgaa cgagcctgtg aagacggtta gtgaagaaac
     2821 cgttgacctt ggtcatgtgg ttgatgctat taaaaaaata gaggaagaag gtcaaggtat
     2881 ttctgatgaa gtcaaggctg aaatcttaaa acatgaaaga catgccagca ctatcttaga
     2941 agaaactggt cacattgaga ttttaaatga acttcaaatc gaggaagcga tgagggaaga
     3001 agcaggcgct gatgaccttg aaactgagca agatcaaact gaaagtcaag aactagaaga
     3061 cttgggcttg aaagttgaaa cgaactttga tattgaacaa gtagctacgg aagtaatggc
     3121 ttatgttcaa acgattattg atgacatgga tgttgaggct acactttcaa atgattataa
     3181 ccgtcgtagc atcaatctac aaattgacac caacgaacca ggtcgtatta tcggctacca
     3241 tggtaaagtc ttgaaggcct tgcaactgtt ggctcaaaat tatctttaca accgctattc
     3301 cagaaccttc tacgttacaa tcaatgtcaa tgattatgtc gaacaccgtg cagaagtctt
     3361 gcagacctat gcgcaaaaat tggcgactcg tgttttggaa gaagggcgca atcataaaac
     3421 agatccaatg tcaaatagcg aacgcaagat tatccatcgt attatttcac gtatggatgg
     3481 cgtgactagt tactctgaag gtgatgagcc aaatcgctat gttgttgtag atacagaata
     3541 agtaaaatca ggtttatcct gattttttgc tagttagagg aggttaaact gatgttgaat
     3601 aagataagag actatttaga ctttgctggt ttgcagtacc gtaatcctga taaagcggga
     3661 gcagagcgag agaagatgct ggcattccgc cacaaaggac aagaggctcg aaaggctttt
     3721 acagaactgg ccaaagcctt tcaagcaagc catccagaat ggcaactcca acagactagt
     3781 cagtggatga atcaggccca gcgtttgaga ccacattttt gggtttatct acagagagac
     3841 ggacaagtga cagaacctat gatagccttg cgtttgtatg gaaatccttc tgattttggt
     3901 atttccttag aagtcagttt catcgaacgt aagaaggatg agcaaacact gggcaagcag
     3961 gccaaagttt tagaaattcc aaccgttaaa gggatttatt atctaaccta ctctaatggt
     4021 caaagtcaac ggtgggaggc gaatgaagaa aagcgtcgta ctttacgcga gaaggtgaga
     4081 agtcaagaag ttcgaaaagt tttagtgaag gtagatgttc ctatgacaga aaattcgtct
     4141 gaagaagaaa tcgtagaagg cttattgaag tcttattcta aaattcttcc ctattatcag
     4201 gtgactagag gataacgtgt cagtcaatta ttataagatt acagtccaag agtgaacagt
     4261 ccgctgtgta attcttggtc tttttgtttg cgctttcgca ttatataata aacttacaaa
     4321 aacaattcaa aaggagaaca attatggaag tcgtttcaaa tgttctaaat tggttttcta
     4381 gcaatatttt gcagaatccc gcatttttcg taggtttatt ggtgttgata ggatatgcac
     4441 ttttgaaaaa acctgcccat gacgtttttt cagggtttgt taaagcaaca gtagggtata
     4501 tgttgcttaa cgtgggtgct ggtggtttgg ttacaacctt tcgtccaatc ttagcagctc
     4561 ttaactacaa attccaaatt ggtgcagcgg ttatcgaccc ttactttgga cttgctgcag
     4621 caaacaacaa aattgcagca gagtttccag attttgttgg aactgcaact acagctctat
     4681 tgattggttt tggaataaat atcttgctcg tagctcttcg aaagattacg aaggtaagaa
     4741 ccctctttat tactggtcac atcatggtac aacaagctgc aacagtatct cttatggttc
     4801 tattcttagt accacaattg cgcaatgctt acggtacagc agcgattggt atcatctgtg
     4861 gactttactg ggcagttagt tcaaatatga ctgttgaggc aactcaacgc ttgactggtg
     4921 gtggcggatt tgcgattggt caccaacagc aatttgcaat ctggtttgta gataaagtag
     4981 caggacgctt tggtaagaaa gaagaaagtt tagacaatct taaattacct aagttcctct
     5041 caatcttcca cgatacagtt gttgcatctg ctaccttgat gctcgtattc ttcggagcca
     5101 ttcttttaat cttgggtcca gacattatgt ctaataaaga agtcatcact tcaggaactc
     5161 tattcaatcc tgctaaacaa gatttcttta tgtacattat ccaaacagcc tttaccttct
     5221 cagtttactt gttcgttttg atgcaaggtg tccgaatgtt cgtatctgag ttgacaaacg
     5281 ccttccaagg tatttcaaac aaattgttgc caggttcatt cccagcggtt gacgttgcag
     5341 cttcttatgg atttggttct ccaaatgctg tcttgtcagg atttaccttt ggtttgattg
     5401 gtcaattgat tataattgtc ttgctcatcg tctttaaaaa tccgattctt attattacag
     5461 gatttgtacc agtgttcttt gacaatgcag ccattgcggt ctacgctgat aaacgcggcg
     5521 gatggaaagc ggctgttatc ctttccttta tatcaggtgt ccttcaagtt gctctaggag
     5581 ctctttgtgt ggcccttctc gatttggcag cttatggtgg ctaccatgga aatatcgact
     5641 ttgaattccc atggcttgga tttggatata tcttcaaata ccttggtatt gttggttatg
     5701 tacttgtgtg tctcttcttg cttgttattc ctcaacttca atttgccaaa gcaaaagata
     5761 aagagaaata ttacaacggt gaagttcaag aagaagctta gtatctagaa aaggagaaat
     5821 aaaatggtta aagtattagc agcgtgcgga aatggaatgg gttcatcaat ggttatcaag
     5881 atgaaggttg aaaatgctct ccgtaagctt aatcaaagag attttacagt caattcatgc
     5941 agtgtcggtg aagctaaagg tttagcagta ggatatgaca tcgtaatcgc ttctcttcat
     6001 ttgattcaag aattggaagg gcgaactaat gggaagttaa ttgggcttga taacttgatg
     6061 gatgataaag aaatcaccga aaaactcagt caagcactac agtaaaaggt tggagggggc
     6121 tggacagaaa ctgagagtta tcgtttctgt ccttctccct cttaaataaa ggaggcagat
     6181 atgaatttaa aacaagcttt aattgacaat gactcgatcc gactaggttt agaggctaac
     6241 aattggaaag aagcagtcaa ggtagcagta gatcccttaa ttgaaagtgg ggcaattttg
     6301 ccagagtatt acgatgctat cattgaatcg actgaagagt atgggcctta ctatatcttg
     6361 atgccaggta tggctatgcc ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt
     6421 tcattgatta ccttacaaaa tcctgttgta ttttcagatg ggaaagaggt atctgttttg
     6481 ttggcactag cagcaacaag ctcaaaaatt cacacaagtg tagccattcc acaaattatt
     6541 gccctgtttg aattagaaga ttctattgca cgtttacagg cttgccagac taaagaagat
     6601 gtcttggcta tgattgaaga atctaaggat agcccttatc tcgaaggatt ggatttggaa
     6661 agttagaaag aggaataaag aaatgacaaa aagaatacct aatttacaag ttgcattaga
     6721 ccattcagac ttgcaaggag cgattaaagc agctgtttct gttggtcagg aagtagatat
     6781 tatcgaagct ggaactgttt gcttgcttca agttggaagt gaactggctg aagtcttgcg
     6841 tagccttttc ccagataaga ttattgtggc agacacaaaa tgtgctgatg ctggtggaac
     6901 agttgctaaa aataatgcgg ttcgtggagc agactggatg acttgtatct gttgtgcaac
     6961 catccctact atggaagcag ctctaaaggc tatcaagact gaacgaggag aacgaggcga
     7021 aatccagatc gagctttatg gcgattggac ttttgaacaa gctcagcttt ggctagatgc
     7081 aggtatctca caagctattt atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg
     7141 gggtgaaaaa gaccttaata aggttaaaaa actcattgac atgggcttcc gtgtatctgt
     7201 aacaggtggt ctagatgtag atactctcaa actctttgaa ggtgttgatg tctttacctt
     7261 tatcgcaggt cgtggaatta cagaggctgc ggatccagca ggagcagcgc gtgccttcaa
     7321 ggatgaaatc aaacgaattt gggggtaaat catggtacgt ccaattggaa tttatgaaaa
     7381 ggcaacccca acacacttta cttggctaga acgtttaaat tttgccaagg agttaggctt
     7441 tgattttgtc gagatgtcta ttgacgaacg tgacgagcgt ttagcaagac ttgactggag
     7501 taaggaagaa cgcttggaag ttgtcaaagc aatctatgga actggtgttc gtattccttc
     7561 tatctgtttt tcaggccatc gtcgctaccc attgggttca aaagatccag ttctagagga
     7621 aaaatctcta gaactcatga aaaaatgtat cgaattagct caagacttgg gagttcgtac
     7681 gattcaatta gctggttacg atgtttacta tgaggaaaag tcaccccaga cacgccaacg
     7741 ttttatcaaa aatttgagaa aagcctgtga ctgggctgaa gaagctcagg tggtacttgc
     7801 tattgaaatt atggatgatc ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa
     7861 agagattgac tctcccttcc tctttgtata tccagatatt ggtaatgtgt ctgcatggca
     7921 taatgatatc tatagtgagt tttatcttgg tcatcatgcc atcgcagctc tccatctcaa
     7981 ggatacttat gcagtgacag aaagttcaag gggccagttc cgagatgtac cttttgggca
     8041 aggttgtgtc aaatgggaag aagctttcga tattttaaag gaaaccaatt ataatggacc
     8101 tttcctaatc gaaatgtggt ctgaaaattg tgaaacagta gaagaaacac gcgcagccat
     8161 tcaagaggcg caagcttttc tctatccact cattaagaaa gcaggtttga tgtaagatga
     8221 atcaagtaat caatgctatg cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac
     8281 atggacttgt caaatttacc tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca
     8341 ttgttatcaa accatcaggc gtggattatg acgaattgac acctgaaaac atggtagtga
     8401 ctgatctaga tggtaagatc ctagaagggg atttaagacc atcttccgac ctcccaactc
     8461 atgtgcaatt atataaggct tggtcagaaa ttggtagtgt ggttcacacc cattcgacag
     8521 aagctgttgg ttgggctcag gcaggtcgtg atattccttt ctacggaaca acccatgcag
     8581 attatttcta cggttcaatc ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg
     8641 cctatgaaaa agatactggc ctggttatcg tagaagaatt tgaacatcgc ggacttaacc
     8701 cggttgaagt accaggaatt gttgtacgca atcacggtcc attcacctgg ggcaaaaatc
     8761 cagagaatgc tgtttatcac tctgtcgtac tagaggaagt atcaaagatg aatcgcttta
     8821 cagaacaaat caatccaaga gttggacctg ctccccagta catactagaa aaacactacc
     8881 aacgtaaaca tggaccaaat gcttattatg gtcaaaagta agaacgatga aggaggagaa
     8941 aaagataaat ttagctcctc tttttacatt tgatttttat tgagagtaaa gttggagttg
     9001 aagtaatttt gaaagatttt ttagaaatag cgcttgatat atatatatat ggtaaaataa
     9061 aaagaattgc tgtgatatca atagatttgg gggatttttt aatatggtac tggataaggc
     9121 aagttgtgat ttacttcaat atttgatgga tcaagaaacg tccaaaacga ttatggcgat
     9181 ttcgaaagat ttgaaagagt caagaaggaa aatttattat cacattgaca aaatcaatgc
     9241 tgctctgggt gacgaggcgc ttcacatcat tagtattcca cgaattggta ttcacttaac
     9301 ggaagagcag agagatgctt gttgtaaact attatcggaa gtagattcgt acgattatat
     9361 catgagtgcg catgaacgta tgatgataat gttattatgg ataggtattt ctaaagaacg
     9421 tattacgatt gaaaaattga tagagttaac agaggtatct aggaatactg ttctcaatga
     9481 tttgaatagt attcgttatc aactaacttt ggaacaatat caggtgacct tgcaagtgag
     9541 caagtcacag ggataccacc ttcatgccca ccctcttaat aaaattcagt atcttcaatc
     9601 gcttctatat catattttta tggaagaaaa tgccactttt gtatctattt tagaagataa
     9661 gatgaaagag aggttagatg atgagtgttt gctttctgtt gaaatgaacc aattttttaa
     9721 ggaacaggtt cctttagttg aacaagattt agggaagaaa ataaaccatc atgaaataac
     9781 ttttatgttg caggttctac cttatttgct gttaagctgt cataatgttg aacagtatca
     9841 agaaagacat caggatatag agaaagaatt ttctttgata agaaaaagaa tagagtatca
     9901 ggtgtctaag aaattaggag aacggttgtt tcaaaagttt gaaatttctt tgtcaggact
     9961 tgaagtttct cttgtagctg ttctcctcct ctcctatcgt aaagatttgg atattcatgc
    10021 agaaagtgat gattttcggc aattaaaact tgctttagaa gaatttatct ggtattttga
    10081 atcacaaatc cgaatggaga ttgagaacaa ggatgatttg ttacgaaatt tgatgatcca
    10141 ctgtaaagcc ttgttattta gaaagactta cggtattttt tctaaaaatc ctctaacaaa
    10201 acaaattcga tccaagtatg gagaattatt tttagtcact agaaaatctg cggaaatttt
    10261 agaaggagca tggtttattc ggctaacaga cgatgatatt gcctatttga cgattcatat
    10321 tggaggattt ttaaaatata caccatcgtc tcaaaaaaat atgaaaaaag tttatctcgt
    10381 ttgtgatgaa ggtgttgcgg tttcgagact tttgctgaaa caatgcaaac tttattttcc
    10441 aaatgagcaa attgacactg tatttacaac agaacaattt aagagtgtgg aagatattgc
    10501 acaagttgat gtagtgatta ctactaatga tgatttggat agcagatttc cgattttaag
    10561 ggttaatcct atccttgaag cagaagatat tttgaaaatg ctagactatc ttaaacacaa
    10621 tatatttcgt aataagagca aaagtttcag tgaaaatctt tctagtctta tttcgtctta
    10681 tattgtagac agcaagttgg ctagtaagtt ccaagaagag gttcaaacac ttataaatca
    10741 agaaatagta gttcaagctt ttttggaaga tatttgaagg acagtccaat gatgaacaca
    10801 aacctgtgtt tttcttggtc ttttttagtg ttttgaaggg tggtatacta atctcaaaga
    10861 taacaattat atccaaagga ggcaacatat gccaaacgtc aaagaaatta caagagagtc
    10921 atggatttta gccactttcc cagagtgggg aacatggttg aacgaagaaa tcgaagaaga
    10981 agtcgtacct gaaggcaact ttgccatgtg gtggctaggc aactgtggta cttggattaa
    11041 gacaccagct ggtgctaacg ttgtcatgga cctttggtca aaccgtggaa aatcaaccaa
    11101 aaaagtgaaa gatatggttc gtgggcacca aatggcaaat atggcaggtg ttcgtaagtt
    11161 gcaaccaaac ttgcgtgttc agccaatggt tatcgatcca tttgctatca acgaactaga
    11221 ctattactta gtttcacact tccacagtga tcatatcgac ccatacacag ctgcagcaat
    11281 tctcaacaat cctaagttag agcatgttaa gtttatcggt ccttaccact gtggacgaat
    11341 ctgggaagga tggggggttc caaaagaacg tatcatcgtt gttaaaccag gtgacactat
    11401 cgaattaaaa gatatgaaga ttcatgcagt agaatcattt gaccgtactt gcttggtaac
    11461 tctcccagtg aacggtgctg atgagacagg cggtgaactt gctggcttgg ctgttacaga
    11521 tgaagaaatg gctcaaaagg ctgttaacta tatctttgaa acaccaggtg gaaccatcta
    11581 tcatggtgca gattctcact tctcaaacta ttttgcaaaa catggtaaag actttaaaat
    11641 tgatgttgct ttgaataact atggtgaaaa tccggtaggt atccaagaca aaatgacatc
    11701 tatcgacctt cttcgtatgg cagaaaatct gcgtaccaaa gtcattatcc cagttcacta
    11761 tgatatctgg tctaacttca tggcttctac taatgagatt ctagaacttt ggaaaatgcg
    11821 aaaagatcgc ttgcaatacg atttccatcc atttatctgg gaagttggcg gtaagtacac
    11881 ttatcctcaa gatcaacact tagtagaata ccatcatcca cgtggttttg atgattgttt
    11941 tgaacaagac tctaacattc aatttaaagc tttgctataa tataaaaata ttctttggag
    12001 gttatccgta tgttgcatga tacggataat tttcatataa tagtaaaata gaatgtgtga
    12061 ttcaataatc acctcaaata gaaaggaaat tctatgtcaa atctatctgt taatgcaatt
    12121 cgttttctag gtattgacgc cattaataaa gccaactcag gtcatccagg tgtggttatg
    12181 ggagcggctc cgatggctta cagcctcttt acaaaacaac ttcatatcaa tccagctcaa
    12241 ccaaattgga ttaaccgcga ccgctttatt ctttcagcag gtcatggttc aatgctcctt
    12301 tatgctcttc ttcacctttc tggttttgaa gatgtcagca tggatgagat taagagcttc
    12361 cgtcaatggg gttcaaaaac accaggtcac ccagaatttg gtcatacggc agggattgat
    12421 gctacgacag gtcctctagg gcaagggatt tcaactgcta ctggttttgc ccaagcagaa
    12481 cgtttcttgg cagccaaata taaccgtgaa ggctacaata tctttgacca ctatacttac
    12541 gttatctgtg gagacggaga cttgatggaa ggtgtctcaa gcgaggcagc ttcatacgca
    12601 ggtttgcaaa aacttgataa gttggttgtt ctttatgatt caaatgatat caacttggat
    12661 ggtgagacaa aggattcctt tacagaaagt gttcgtgacc gttacaatgc ctacggttgg
    12721 catactgcct tggttgaaaa tggaacagac ttggaagcca tccatgctgc tatcgaaaca
    12781 gcaaaagctt caggcaagcc atctttgatt gaagtgaaga cggttattgg atatggttct
    12841 ccaaacaaac aaggaactaa tgctgtacac ggcgcccctc ttggagcaga tgaaactgca
    12901 tcaactcgcc aagccctcgg ttgggactac gaaccatttg aaattccaga acaagtatat
    12961 gctgatttca aagaacatgt tgcagaccgt ggcgcatcag cttatcaagc ttggactaaa
    13021 ttagttgcag attataaaga agctcatcca gaactggctg cagaagtaga agccatcatc
    13081 gacggacgtg atccagtcga agtgactcca gcagacttcc cagctttaga aaatggtttt
    13141 tctcaagcaa ctcgtaattc aagtcaagat gccttgaatg ttgtggcagc taaattacca
    13201 accttcctag gtggatcagc tgaccttgct cactcaaaca tgacttatat caagacggat
    13261 ggacttcaag acgataccaa tcgcttgaat cgtaacattc agtttggtgt tcgtgaattt
    13321 gcaatgggaa caatcttgaa cgggatggcc cttcatggtg gacttcgtgt atacggtgga
    13381 actttcttcg tcttctctga ctatgtgaag gcagctgtcc gcttgtcagc cttacaagga
    13441 cttcctgtga cttatgtctt tacccatgat tcaatcgcag ttggggaaga tggtccgact
    13501 cacgaaccag ttgagcattt agcaggtctt cgtgctatgc caaatctaaa tgttttccgt
    13561 ccagcagatg cgcgtgaaac gcaagcagct tggtaccttg cagtgacaag tgagaaaaca
    13621 ccaactgccc ttgtcttgac acgtcaaaac ttgactgttg aagatggaac agacttcgac
    13681 aaggttgcta aaggtgctta tgttgtatat gaaaatgcag ccgactttga taccatcttg
    13741 attgcgacag gttcagaggt taatcttgct gtctcagctg ctaaagaatt ggctagtcaa
    13801 ggcgaaaaaa tccgcgtagt cagcatgcca tctacagatg tctttgataa acaagatgca
    13861 gcttacaagg aagaaattct tccaaatgca gtccgccgtc gtgttgcagt cgaaatgggt
    13921 gcaagtcaaa actggtacaa atatgttggt ctcgatggtg ccgttctagg tattgatacc
    13981 ttcggagcct ctgccccagc accaaaagta ttggcagaat atggctttac tgtcgaaaat
    14041 cttgtaaaag ttgttcgaaa tttgaaataa tcctaaaaat cagggcgtaa gctctggttt
    14101 ttcttaccag aaaagtaagg tacaatcttg taaaagtagc tgaaatttga tatagtagtc
    14161 ctatgtaaaa gacaaaggag aaaacaatga atccaaatat tactttttta atcatgcttg
    14221 taggtatgat ggccttgatg ttctttatgc aacgttctca aaagaaacaa gctcaaaagc
    14281 gtatggaaag cttaaataaa ctacaaaaag gctatgaagt gattacaatc ggtggacttt
    14341 atggaacagt cgatgaagta gatacggaga agggaacaat agttcttgac gtagatggag
    14401 tttacttgac ttttgaacta gctgctatca agacagtatt accgctgaaa gaaacagctt
    14461 cactagaagg cgcaattgaa aaataagacg ggatcacgaa ctcccgtttt ctataaaaga
    14521 aaggaaatgg gatgaaaaaa ttagtctttg tctgtctggg aaatatttgc cgtagcccta
    14581 tggccgagtt tgttatgaaa tcaatgacag ataactacga aatccaaagt cgagcaactt
    14641 cctcttggga acatggcaat ccgattcata aggggactca gggaattttt caagagtatg
    14701 agattcctta tgacaagaac aagacatcgc ttcagattag taaggaagat tttgaagcct
    14761 ttgattatat tatcggaatg gacgcttcaa atatttctga cttacgtcag atgtgtccag
    14821 tagactgtca agataagatt tactcatttt catctgaaag tgttccagac ccttggtata
    14881 caggagattt tgaagaaacc tatcgacgtg ttcaagaggg ctgtcaagct tggttagaac
    14941 gtttagaaaa ggagagttaa ggtggaaaat cttacgaatt tttacgaaaa gtatcgtgtc
    15001 tatctgactc gtccacgttt agagcttttg gcagtagtta ccattgtttt ctgtgctgta
    15061 ctcgtctttt ttctaaatat tccaggaaaa ggtgtcttaa aactcgataa tggaacgatt
    15121 gtttatgatg gcagtcttgt ccgtggtaaa atgaatggcc aaggtaccat taccttccaa
    15181 aatggagacc aatatacagg tggcttcaac aatggagcct tcaacggaaa aggtaccttt
    15241 caatctaaag aaggctggac ctacgaaggt gattttgtaa atggtcaggc tgaaggaaaa
    15301 gggaaactaa caacagaaca agaagtcgtt tatgaaggaa cttttaaaca aggcgttttt
    15361 caacaaaaat aaagcctcct tatcaaagga ggtattatta gaatcacaag gtaagcgctt
    15421 acctgtaaat ccctttcttt ccaaatccct cttccaagca agtttgtgaa ataaaaaata
    15481 tttgaaataa atttcacaaa cttcaaagat aaaacttgat aagaaaagaa aatgagaaaa
    15541 gtttcacaag agtttaaaaa ttctttgtga aatgtttatg aaactgttta caaagttgaa
    15601 ataatgcgtt ataataaact tgtgaaaaaa ttaacaaagg atatcgttcc ttgaaagcta
    15661 tggaggaaaa tatggctgat aaaaaaactg tgacaccaga ggaaaagaaa ctcgttgctg
    15721 aaaaacacgt agatgagttg gttcaaaaag ctctagttgc ccttgaagaa atgcgtaaat
    15781 tggatcaaga acaagttgac tacatcgttg ccaaagcatc agtagcagct ttggatgccc
    15841 acggagaatt ggctttacat gcctttgaag aaacaggacg tggtgtattt gaagacaaag
    15901 caactaagaa cttgtttgcc tgtgaacacg tagtaaacaa catgcgccac actaagacag
    15961 ttggcgttat cgaagaagac gatgtaacag gattgactct tattgctgaa ccagttggtg
    16021 ttgtttgtgg tattactcca acaacaaacc caacatcaac agcaatcttc aaatcattga
    16081 tttcattgaa gacacgtaac ccaatcgtct ttgccttcca tccatcagca caagaatcat
    16141 ctgctcatgc agctcgtatc gtccgcgatg cagctatcgc agctggtgct cctgaaaact
    16201 gtgtgcaatg gattactcaa ccatctatgg aagcaacaag tgcccttatg aaccacgaag
    16261 gtgttgcgac aatccttgca acaggtggta atgccatggt taaggcggct tattcatgtg
    16321 gtaaaccagc tcttggggta ggtgccggaa acgttccagc ttatgttgaa aaatcagcaa
    16381 acattcgtca agcagcacac gatatcgtca tgtctaaatc atttgataac ggtatggtct
    16441 gtgcatctga acaagcagtt atcattgata aagaaattta cgatgaattt gtagcagagt
    16501 tcaaatctta ccacacttac tttgtaaaca aaaaagaaaa agctcttctt gaagagttct
    16561 gcttcggcgt caaagcaaac agcaaaaact gtgctggtgc aaaattgaac gctgacatcg
    16621 ttggtaaacc agcaacttgg attgcagaac aagcaggatt tacagttcca gaaggaacaa
    16681 acattcttgc tgcagaatgt aaagaagttg gcgaaaatga gccattgact cgtgaaaaat
    16741 tgtcaccagt tattgcagtt ttgaaatctg aaagccgtga agatggtatt actaaggctc
    16801 gtcaaatggt tgaatttaac ggtcttggac actcagcagc tatccacaca gctgacgaag
    16861 aattgactaa agaatttggt aaagctgtta aagctattcg tgttatctgt aactcacctt
    16921 ctacttttgg tggtatcggg gacgtttaca atgccttctt gccatcattg acacttggat
    16981 gtggttctta cggacgcaac tcagttgggg ataacgttag tgccattaac ctcttgaata
    17041 tcaaaaaagt cggaagacgg agaaataaca tgcaatggat gaaacttcct tcaaaaacat
    17101 actttgaacg tgattcaatt caataccttc aaaaatgtcg tgacgttgaa cgtgtcatga
    17161 tcgttactga ccatgccatg gtagagcttg gtttccttga tcgtatcatc gaacaactgg
    17221 accttcgtcg caataaggtt gtttaccaaa tctttgcgga tgtagaaccg gatccagata
    17281 tcacaactgt aaaccgtggt actgagatta tgcgtgcctt caaaccagat accatcatcg
    17341 cactcggtgg tgggtctcca atggatgctg ccaaagtaat gtggctcttc tacgagcaac
    17401 cagaagtgga cttccgtgac cttgtccaaa aattcatgga tatccgtaaa cgtgccttca
    17461 agttcccatt gcttggtaag aagactaaat tcatcgcgat tccaactaca tctggtacag
    17521 gatctgaagt aacaccattt gccgttatct ctgataaagc aaacaaccgt aaatacccaa
    17581 tcgctgacta ctcattgaca ccaactgtgg caatcgtaga tcctgctttg gtattgacag
    17641 ttccaggatt tgttgctgct gatactggta tggacgtatt gactcacgcg acagaagcat
    17701 acgtatcaca aatggctagt gactacactg atggtttagc acttcaagcc attaaattgg
    17761 tctttgaaaa tctcgaaagc tcagttaaga atgcagactt ccactcacgt gagaaaatgc
    17821 ataacgcttc aacaatcgct ggtatggcct ttgccaatgc cttcctaggt atttctcact
    17881 caatggccca taagattggt gcgcaattcc acacaatcca cggtcgtaca aatgccatct
    17941 tgcttccata cgttatccgt tacaacggta cacgtccagc taagacagca acatggccta
    18001 agtacaacta ctaccgtgca gatgaaaaat accaagatat cgcacgcatg cttggacttc
    18061 cagcttctac tccagaagaa ggggttgaat cttacgcaaa agctgtctac gaactcggtg
    18121 aacgtattgg gatccaaatg aattttagag accaaggaat tgacgaaaaa gaatggaaag
    18181 aacattctcg tgaattagcc ttcctggctt atgaagacca atgttcacca gctaacccac
    18241 gtcttccaat ggtagaccat atgcaagaaa tcatcgaaga tgcatactat ggctacaaag
    18301 aaagaccagg acgccgtaaa taattgttta tcagtctaga agcaagacaa aaactcaatt
    18361 tgagggaaag aaccagtaat ttttctatga taaaaggcat cctatcaagg tttttgaata
    18421 cctgatagga tgccttttta tgatgttgag gcctttttgc cctttttgaa aaactagaat
    18481 agaaacaaaa tatatagtag gttgaaacta gaatagtaca catctgcttc taaaacattg
    18541 ttagaattcg atttgactgt cctgat
//
LOCUS       DAVHIU010000031        18302 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVHIU010000031 DAVHIU010000000
VERSION     DAVHIU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.033"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            16..1989
                     /locus_tag="VVN06_000908"
     CDS             16..1989
                     /locus_tag="VVN06_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5562486.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            1986..2438
                     /gene="rplI"
                     /locus_tag="VVN06_000909"
     CDS             1986..2438
                     /gene="rplI"
                     /locus_tag="VVN06_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5562487.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            2482..3834
                     /gene="dnaB"
                     /locus_tag="VVN06_000910"
     CDS             2482..3834
                     /gene="dnaB"
                     /locus_tag="VVN06_000910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5562488.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            3836..4102
                     /locus_tag="VVN06_000911"
     CDS             3836..4102
                     /locus_tag="VVN06_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562489.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            4191..5537
                     /gene="cbpD"
                     /locus_tag="VVN06_000912"
     CDS             4191..5537
                     /gene="cbpD"
                     /locus_tag="VVN06_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU5562490.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      5620..5722
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5793..6083
                     /locus_tag="VVN06_000913"
     CDS             5793..6083
                     /locus_tag="VVN06_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU5562491.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            6046..6804
                     /locus_tag="VVN06_000914"
     CDS             6046..6804
                     /locus_tag="VVN06_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562492.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            6842..7849
                     /locus_tag="VVN06_000915"
     CDS             6842..7849
                     /locus_tag="VVN06_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5562493.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            7849..8577
                     /locus_tag="VVN06_000916"
     CDS             7849..8577
                     /locus_tag="VVN06_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562494.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            8696..9154
                     /locus_tag="VVN06_000917"
     CDS             8696..9154
                     /locus_tag="VVN06_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU5562495.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLVSVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            9156..11588
                     /locus_tag="VVN06_000918"
     CDS             9156..11588
                     /locus_tag="VVN06_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5562496.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            12230..12883
                     /gene="rr06"
                     /locus_tag="VVN06_000919"
     CDS             12230..12883
                     /gene="rr06"
                     /locus_tag="VVN06_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU5562497.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            12880..14211
                     /gene="hk06"
                     /locus_tag="VVN06_000920"
     CDS             12880..14211
                     /gene="hk06"
                     /locus_tag="VVN06_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU5562498.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            14306..14833
                     /locus_tag="VVN06_000921"
     CDS             14306..14833
                     /locus_tag="VVN06_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU5562499.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            14912..16951
                     /gene="pspC"
                     /locus_tag="VVN06_000922"
     CDS             14912..16951
                     /gene="pspC"
                     /locus_tag="VVN06_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU5562500.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTKTVALINELQNIKKTYLY
                     NLNVLKEKSELPSKIKAKLEVAFDQFKKDTLKPGEKVAEAEKKVAEAKKKAEDQKEQD
                     RRNYPTNTYKTLELEIAESDVKVKEAELELVKEEAKESRNEEKVKQAKAKVESEKAEA
                     IRLEEIKTDREEAKRKADAKLKEAVENNAATSEQGEPKRRVKRGVLGEPATPDKKEND
                     AKSSDSSVGEETLPSPSLKPEKKVAEAEKKVAEAEKKAKDQKEEDRRNYPTNTYKTLE
                     LEIAESDVKVKEAELELVKEEAKESRNEEKVKQAKAKVESKKAEATRLEKIKTDRKKA
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPKPEKPAPAPKPENPAEQPKAEKPADQQAEED
                     YARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNG
                     SWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGSMATGWLQY
                     NGSWYYLNANGDMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATDWV
                     KDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(17154..18302)
                     /locus_tag="VVN06_000923"
                     /pseudo
     CDS             complement(17154..18302)
                     /locus_tag="VVN06_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5795 a         3006 c         4240 g         5261 t
ORIGIN      
        1 gccaagaggt tttgaatgaa aaaattttat gtaagtccaa tttttcctat tctagtagga
       61 ttgattgcgt ttggagtctt atccactttc attatttttg ttaataataa tctgttgacg
      121 gttttaattt tgtttctttt tgtaggaggc tatgtttttt tatttaagaa actgagagtg
      181 cattatacaa ggagtgatgt agaacagata cagtatgtaa accaccaagc ggaagaaagt
      241 ttgacagctc tattggaaca gatgcctgta ggtgttatga aattgaattt atcttctgga
      301 gaggttgagt ggtttaatcc ctatgctgaa ttgattttga ccaaggaaga tggtgatttt
      361 gatttagaag ctgttcaaac gattatcaag gcttcagtag gaaatccgtc tacttatgcc
      421 aagcttggtg agaagcgtta tgctgttcat atggatgctt cttccggtgt tttgtatttt
      481 gtagatgtat ccagggaaca agccataaca gatgaattgg taacaagtag accagtgatt
      541 gggattgtct ctgtggataa ttatgatgat ttggaggatg caacttctga gtcagatatt
      601 agtcaaatca atagttttgt agctaatttt atatcagagt tttcagaaaa atacatgatg
      661 ttttctcgtc gggtaagtat ggatcgattt tatctattta ctgactacac ggtgcttgag
      721 ggcttgatga atgataaatt ttctgttatt gatgctttca gagaagagtc gaaacagaga
      781 cagttgccct tgaccttaag tatgggattt tcttatggcg atggaaatca tgatgagata
      841 gggaaagttg ctttgctcaa tttgaacttg gctgaagtac gtggtggcga ccaggtggtt
      901 gttaaggaaa acgacgaaac gaaaaatcca gtttattttg gtggtgggtc tgctgcttca
      961 atcaagcgta cacggactcg tacgcgcgct atgatgacag ctatttcaga taagattcgg
     1021 agtgtagatc aggtttttgt agtcggtcac aaaaatttag acatggatgc tttgggctct
     1081 gctgtaggta tgcagttgtt cgccagcaat gtgattgaaa atagctatgc tctttatgat
     1141 gaagaacaaa tgtctccaga tattgaacga gctgtttcat tcatagaaaa agaaggagtt
     1201 acgaagttgt tgtctgttaa ggatgcaatg gggatggtga ccaatcgttc tttgttgatt
     1261 cttgtagacc attcaaagac agccttaaca ttatcaaaag aattttatga tttatttacc
     1321 caaaccattg ttattgacca ccatagaagg gatcaggatt ttccagataa tgcggttatt
     1381 acttatatcg aaagtggtgc aagtagtgcc agtgagttgg taacggaatt gattcagttc
     1441 cagaattcta agaaaaatcg tttgagtcgt atgcaagcaa gtgttttgat ggctggtatg
     1501 atgttggata ctaaaaattt cacctcgcga gtaactagtc ggacatttga tgttgctagc
     1561 tatctcagaa cgcgcggaag tgatagtatt gctatccagg aaatcgctgc gacagatttt
     1621 gaagaatatc gtgaggttaa tgaacttatt ttacaggggc gtaaattagg ttcagatgta
     1681 ctaatagcag aggctaagga cacgaaatgc tatgatacag ttgttattag taaggcagca
     1741 gatgccatgt tagccatgtc aggtattgaa gcgagttttg ttcttgcgaa gaatacacaa
     1801 ggatttatct ctatctcagc tcgaagtcgt agtaaactga atgtacaacg gattatggaa
     1861 gagttaggcg gtggaggcca ctttaatttg gcagcagctc aaattaaaga tgtaaccttg
     1921 tcagaagcag gtgaaaaact gacagaaatt gtattaaatg aaataaagga aaaggagaaa
     1981 gaagaatgaa agtaatcttt ttagcagatg ttaaaggaaa aggtaaaaaa ggcgaaatta
     2041 aggaagtacc aacagggtat gcgcaaaact ttcttatcaa aaagaatcta gccaaagaag
     2101 cgactgctca agctgtaggt gaacttcgtg gtaaacaaaa atcggaagaa aaagctcacg
     2161 ctgagatgat tgcagaaggg aaagcaatta aagcacaact tgaagcagaa gaaactgttg
     2221 tagaatttgt tgaaaaagtt ggtccagatg gtcgtacctt tggttctatt accaataaga
     2281 agattgcaga agaattacaa aagcaatttg gaattaagat tgataaacgt catattcaag
     2341 tacaagctcc gattcgagcg gttggtttga ttgatgtgcc agtgaaaatc tatcaagata
     2401 tcacaagtgt aatcaatctt cgtgtgaaag aaggataagt ttacaccttc ttgacaagat
     2461 tgtaaaagga agggaagtct gatggcagaa gtagaagagt tacgagtaca acctcaagat
     2521 atcttagctg agcaatccgt tttaggggct atctttattg atgagagtaa acttgttttt
     2581 gtgcgagaat acattgagtc tcgggacttt tttaagtatg cccatcgttt gattttccaa
     2641 gccatggtcg atttatccga tcgtggtgat gccatagatg caacaacggt tcgtactatc
     2701 cttgataatc aaggtgattt acagaatatt ggtggcttgt cttacttggt tgagattgtc
     2761 aattctgtgc caacttctgc taatgcggag tattatgcta agattgttgc agaaaaagca
     2821 atgctacgtc gtttaattgc caagttgaca gagtctgtca accaagctta cgaagcgtca
     2881 caaccagctg atgaaattat tgctcaggca gaaaaagggc tgattgatgt cagtgaaaat
     2941 gcaaatcgaa gcgggtttaa gaacattcga gatgtgttga atctcaactt tggaaatctg
     3001 gaagctcgct cgcaacaaac gaccgatatt acaggtattg cgacaggtta tcgtgatttg
     3061 gatcatatga caacaggact tcatgaggag gagttgatta tcttagcagc tcgtccagca
     3121 gttggtaaga cagcatttgc cttgaatatc gctcagaata ttgggactaa gttggacaaa
     3181 acggttgcta ttttttcact cgaaatgggt gcggaaagct tggtagaccg tatgttagct
     3241 gcagaaggct tggtggagtc acattctatc cgtacagggc aattgacaga tgaggagtgg
     3301 caaaaatata ctattgctca gggtaatcta gctaacgcca gtatctatat cgatgatacg
     3361 ccaggtattc ggattacaga gattcgttct cgttctcgta aattggctca agaaactgga
     3421 aatcttggtt tgattttgat agactatttg caacttatca cgggaactgg tcgagaaaat
     3481 cgtcaacaag aagtttctga aatttctcgt cagttgaaaa tactagccaa ggaattgaag
     3541 gttccagtaa tcgctctgag tcagctttct cgtggtgtag aacaacgtca ggacaagaga
     3601 ccggtcttgt ctgatattcg tgaatctggg tctattgagc aggacgctga tatcgtagct
     3661 tttctctatc gcgatgacta ctatgaacgt ggtggtgaag aagaggaggg tatcccaaat
     3721 aataaggtgg aagttattat cgagaaaaac cgtagtggag ctcgtggaac agtggaattg
     3781 attttccaaa aagaatacaa taaattttca agtatctcaa agagggaggc ataagatgtc
     3841 agatgcattt acagatgtag ccaagatgaa aaaaatcaaa gaagaaatca aggcacatga
     3901 gggacaagtc gtagaaatga ctttggagaa tggtcgtaag cgccaaaaaa atagattggg
     3961 taagctaatt gaagtttatc catccctatt tattgtggag tttggggatg tggaaggaga
     4021 taaacaagtt aatgtttacg ttgaatcctt tacttactca gatattctta cagaaaagaa
     4081 tttgattcat tatcttgact aaagtgagaa attttctcac tttttctttt ttctccgaat
     4141 aatttaggtg aaggcaatca tcgctttata ttatttttta aggaggaaga atgaaaattt
     4201 taccgtttat agcaagagga acaagttatt acttgaagat gtcagttaaa aagcttgttc
     4261 cttttttagt agtaggattg atgctagcag ctggtgatag tgtctatgcc tattccagag
     4321 gaaatggatc gattgcgcgt ggggatgatt atcctgctta ttataaaaat gggagccagg
     4381 agattgatca gtggcgcatg tattctcgtc agtgtacttc ttttgtagcc tttcgtttga
     4441 gtaatgtcaa tggttttgaa attccggcag cttatggaaa tgcgaatgaa tggggacatc
     4501 gtgctcgtcg ggaaggttat cgtgtagata atacaccgac gattggttcc attacttggt
     4561 ctactgcagg aacttatggt catgttgcct gggtgtcaaa tgtaatggga gatcagattg
     4621 agattgagga atataactat ggttatacag aatcctataa taaacgaatt ataaaagcaa
     4681 acacgatgac aggatttatt cattttaaag atttggatgg tggcagtgtt gggaatagtc
     4741 aatcctcaac ttcaacaggc ggaactcatt attttaagac caagtctgct attaaaactg
     4801 aacccctagt tagtgcaact gtgattgatt actattatcc tggagagaag gttcattatg
     4861 atcaaattct cgaaaaagac ggctacaagt ggttgagtta tactgcctat aatggaagct
     4921 atcgttatgt tcaattggag gctgtgaata aaaatcctct aggtaattct gttctttctt
     4981 caacaggtgg aactcattat tttaagacca agtctgctat caaaactgaa cccctagtta
     5041 gtgcaactgt gattgattac tattatcctg gagagaaggt tcattatgat cagatacttg
     5101 aaaaagacgg ctacaagtgg ttgagttata cggcttataa cggaagtcgt cgctatatac
     5161 agctagaggg agtgacttct tcacaaaatt atcagaatca atcaggaaat atctctagct
     5221 atggatccaa taatagttca actgtcggtt ggaagaaaat aaatggtagt tggtatcatt
     5281 tcaaatcaaa tggttctaaa tcaacaggat ggctgaaaga tggttctagc tggtattatt
     5341 tgaaattatc tggtgaaatg cagacaggat ggttaaagga gaatggctcg tggtattatc
     5401 tgggtagttc aggggcaatg aaaacaggct ggtaccaggt ctctggtaag tggtattatt
     5461 cttactcttc aggcgcctta gctgttaata cgacggtgga tggctacaga gtaaacagtg
     5521 atggagaacg agtatagaaa attggagtag gagaaatttc ctgctctttt ctgtaagcag
     5581 tttccttgac atttttctta ttttgcgcta tcatatatac atataatata tgggagtctg
     5641 tgtacagtct gagaggaagt gttaaacttc gaccgcacct gatctgggta atgccagcgg
     5701 agggaacgat acttagtcta attttgcacc ttttccatgt atggtaaagg tttttctttt
     5761 ttttaaaagg aaaacgagaa gaggaggttc ttatgaaagc aagcattgcc ttgcaagttt
     5821 tacccctagc acaggggatt gatcggatag ctgttattga tcaggtcatt gcttatctgc
     5881 aaactcaaga agtgacgatg gtagtgacac catttgaaac ggtcttggaa ggggagtttg
     5941 atgagcttat gcgcattcta aaagaagcgc tggaagtggc agggcaggag gcagacaatg
     6001 tctttgccaa tgtcaaaata aatgtaggag agattttaag tattgatgag aaacttgaaa
     6061 agtatactga gacgacacat tagtctattg ggctttctcg gagtattgtc aatctggcag
     6121 ttagcaggtt ttcttaaact tctccccaag tttatcctgc cgacacctct tgaaattctc
     6181 cagccctttg ttcgtgacag agaatttctc tggcaccata gctgggcgac cttgagagtg
     6241 gctttactgg ggctgatttt gggagttttg attgcctgtc ttatggctgt gctcatggat
     6301 agtttgactt ggctcaatga cctgatttac cctatgatgg tggtcattca gaccattccg
     6361 accattgcca tagctcctat cctggtcttg tggctaggtt atgggatttt gcccaagatt
     6421 gtcttgatta tcttaacgac aacctttccc atcatcgtta gtattttgga cggttttagg
     6481 cattgtgaca aggatatgct gaccttgttt agtctgatgc gggccaagcc ttggcaaatc
     6541 ctgtggcatt ttaaaatccc ggttagcctg ccttactttt atgcaggtct gagggtcagt
     6601 gtctcctacg cctttatcac aactgtggta tctgagtggt tgggaggttt tgaaggtctt
     6661 ggtgtttata tgattcagtc taaaaaactg tttcagtatg ataccatgtt tgccattatt
     6721 attctggtgt cgattatcag tcttttgggt atgaagctgg tcgatatcag tgaaaaatat
     6781 gtgattaaat ggaaacgtac ttaaaataga gcgtttttga aaaagaaaag aggaaatcaa
     6841 aatgaagaaa acatggaaag tgtttttaac gcttgtaaca gctcttgtag ctgttgtgct
     6901 tgtggcctgt ggtcaaggaa ctgcttctaa agacaacaaa gaggcagaac ttaagaaggt
     6961 tgactttatc ctagactgga caccaaatac caaccacaca gggctttatg ttgccaagga
     7021 aaaaggttat ttcaaagaag ctggagtgga tgttgatttg aagttgccac cagaagaaag
     7081 ttcttctgac ttggttatca acggaaaggc accatttgca gtgtatttcc aagactacat
     7141 ggctaagaaa ttggaaaaag gagcaggaat cactgccgtt gcagctattg ttgaacacaa
     7201 tacatcagga atcatctctc gtaaatctga taatgtaagc agtccaaaag acttggttgg
     7261 taagaaatat gggacatgga atgacccaac tgaacttgct atgttgaaaa ccttggtaga
     7321 atctcaaggt ggagactttg agaaggttga aaaagtacca aataacgact caaactcaat
     7381 cacaccgatt gccaatggcg tctttgatac tgcttggatt tactacggtt gggatggtat
     7441 ccttgctaaa tctcaaggtg tagatgctaa cttcatgtac ttgaaagact atgtcaagga
     7501 gtttgactac tattcaccag ttatcatcgc aaacaacgac tatctgaaag ataacaaaga
     7561 agaagctcgc aaagtcatcc aagccatcaa aaaaggctac caatatgcca tggaacatcc
     7621 agaagaagct gcagatattc tcatcaagaa tgcacctgaa ctcaaggaaa aacgtgactt
     7681 tgtcatcgaa tctcaaaaat acttgtcaaa agaatacgca agcgacaagg aaaaatgggg
     7741 tcaatttgac gcagctcgct ggaatgcttt ctacaaatgg gataaagaaa atggtatcct
     7801 taaagaagac ttgacagaca aaggcttcac caacgaattt gtgaaataat gacagaaatt
     7861 agactagagc acgtcagtta tgcctatggt caggagagga ttttagagga tatcaaccta
     7921 caggtgactt caggcgaagt ggtttccatc ctaggcccaa gtggtgttgg aaagaccacc
     7981 ctctttaatc taatcgctgg gattttagaa gttcagtcag ggagaattgt ccttgatggt
     8041 gaagaaaatc ccaaggggcg cgtgagttat atgttgcaaa aggatctgct cttggagcac
     8101 aagacggtgc ttggaaatat cattctgccc ctcttgattc aaaaggtgga taaggcagaa
     8161 gctatttccc gagcggataa aattcttgcg accttccagc tgacagctgt aagagacaag
     8221 tatcctcatg aacttagcgg tgggatgcgc cagcgtgtag ccttactccg gacctacctt
     8281 tttgggcaca agctctttct cttagatgag gcctttagcg ccttggatga gatgacaaag
     8341 atggaactcc acgcttggta tcttgagatt cacaagcagt tgcagctaac aaccctgatc
     8401 atcacgcata gtattgagga ggccctcaat ctcagcgacc gcatctatat cttgaaaaat
     8461 cgccctgggc agattgtttc agaaattaaa ctagattggt ctgaagatga ggacaaggaa
     8521 gtccaaaaga ttgcctacaa acgtcaaatt ttggcggaat taggcttaga taagtagaaa
     8581 aatagggagt tggtgaagat tatcctttac cagcgccctt tttcttttaa aaatgagaaa
     8641 atttcggtat aatagtcaaa caaggtcaag gtttaaagag agaggtgggt ttgttatgag
     8701 atttaaaaat acatcggatc atattgaggc ctacatcaag gcgattttag atcaatctgg
     8761 tatcgtggag ttgcaacgga gtcagttggc agataccttt caggttgttc ctagtcagat
     8821 taactacgtg atcaagacac gctttacgga aagtagaggc tacttggttg aaagtaagcg
     8881 tggtggcgga ggctacattc gtataggacg gattgagttt tctagtcatc atgaaatgct
     8941 ccgggagctg ctttactcga ttggtgagcg agtcagtcaa gaaatttatg aggatattct
     9001 ccagcttttg gttgagcagg aattgatgac caagcaggag atgaatttgc tagtatcagt
     9061 agctttggat cgcgttttag gagaagaagc tccagttgtt cgagcaaaca tgctacgtca
     9121 gatcatacaa gaagtagata gaaaagggaa gtaagatgaa ctattcaaaa gcattgaatg
     9181 aatgtatcga aagtgcctac atggttgctg gacattttgg agctcgttat ctagagtctt
     9241 ggcacttgtt gattgccatg tctaatcaca gttatagtgt agcaggggca actttaaatg
     9301 attatccgta tgagatggac cgtttagaag aggtggcttt ggaactgact gaaacggact
     9361 atagccagga tgaaaccttt acggaattgc cgttctcccg tcgtttgcag gttctttttg
     9421 atgaagcaga gtatgtagcg tcagtggtcc atgctaaggt actagggaca gagcacgtcc
     9481 tctatgcgat tttgcatgat ggcaatgcct tggcgactcg tatcttggag agggctggtt
     9541 tttcttatga agacaagaaa gatcaggtca agattgctgc tcttcgtcga aatttagaag
     9601 aacgggcagg ctggactcgt gaagatctca aggctttacg ccaacgccat cgtacagtag
     9661 ctgacaagca aaattctatg gccaatatga tgggcatgcc gcagactcct agtggtggtc
     9721 tcgaggacta tacgcatgat ttgacagagc aagcgcgttc tggcaagtta gaaccagtca
     9781 tcggtcggga caaggaaatc tcacgtatga ttcaaatctt gagccgtaag actaagaaca
     9841 accctgtctt ggttggggat gctggtgtcg ggaaaacagc tctggcgctt ggtcttgctc
     9901 agcgtattgc tagtggtgac gtgcctgcgg aaatggctaa gatgcgcgtg ttagaacttg
     9961 atttgatgaa tgtcgttgca gggacacgct tccgtggtga ctttgaagaa cgcatgaata
    10021 atatcatcaa ggatattgaa gaagatggcc aagtcatcct ctttatcgat gaacttcaca
    10081 ccatcatggg ttctggtagc gggattgatt cgactctgga tgcggccaat atcttgaaac
    10141 cagccttggc gcgtggaact ttgagaacgg ttggtgccac cactcaggaa gaatatcaaa
    10201 aacatatcga aaaagatgca gcactttctc gtcgtttcgc taaagtgacg attgaagaac
    10261 caagtgtggc agatagtatg actattttac aaggtttgaa ggcgacttat gagaaacatc
    10321 accgtgtaca aatcacagat gaagcggttg aaacagcggt taagatggct catcgttatt
    10381 taaccagtcg tcacttgcca gactctgcta tcgatctctt ggatgaggcg gcagcaacag
    10441 tgcaaaataa atcaaagcat gtaaaagcag acgattctga cttgagtcca gctgacaagg
    10501 ccttgatgga tggcaagtgg aaacaggcag cccagctaat cgcaaaagaa gaggaagtgc
    10561 ctgtctataa agacttggtg acagaatctg atattttgac caccttgagt cgcttgtcag
    10621 gtatccccgt tcaaaaactg actcagactg atgccaagaa atacttaaac ttggaagctg
    10681 aactgcacaa acgtgtcatc ggtcaagacc aagctgtttc aagtattagt cgtgccattc
    10741 gccgcaatca gtcaggaatt cgcagtcaca agcgtccgat cggttccttt atgttcctag
    10801 gacccacagg tgtcggtaag accgagttgg ccaaggcttt ggcagaagtt ctctttgatg
    10861 acgaatcagc ccttatccgc tttgatatga gtgagtatat ggagaaattc gcagccagcc
    10921 gtctaaatgg agctcctcca ggctatgtgg gttacgaaga aggtggggag ttgacagaga
    10981 aggttcgcaa taaaccctat tccgttctcc tctttgatga ggtagagaag gcccatccag
    11041 atatctttaa tgttctcttg caggttctgg atgacggtgt cttgacagat agcaagggac
    11101 gcaaggtcga tttttcaaat accattatca ttatgacatc gaacttaggt gcgactgccc
    11161 ttcgtgatga taagactgtt ggttttgggg ctaaggatat tcgttttgac caggaaaata
    11221 tggaaaaacg catgtttgaa gaactgaaaa aagcttatag accggaattc atcaaccgta
    11281 ttgatgagaa ggtggtcttc catagcctat ctagtgacca tatgcaggaa gtggtgaaga
    11341 ttatggtcaa gcctttagtg gcaagtttgg ctgaaaaagg cattgacttg aaattacaag
    11401 cttcagctct gaaattgtta gcaaatcaag gatatgaccc agagatggga gctcgcccac
    11461 ttcgcagaac cctgcaaaca gaagtggagg acaagttggc agaacttctt cttaagggag
    11521 atttagtggc aggcagcaca cttaagattg gtgtcaaagc aggccagtta aaatttgata
    11581 ttgcataaaa gaataaaagt atcagcatct gaccataagt cacagtggag tgaaactcaa
    11641 tgaaaatcaa agagcaaact aggcagctag ccgcaggttg ctcaaaacac tggtttgagg
    11701 ttgcagatag agctgacgtg gtttgaagag attttcgaag agtatgaaac taaaacctat
    11761 agcttctaaa cgatccgtgg ttttcaccat tcaacacaaa attcatatgt ttattaccct
    11821 ccgtcgtatt tgtcttagag cgtgtgtagt agaaaaagag cagtcttatc tgaaattttt
    11881 attctttcaa aagagacctg tttctttttt gcatgtcaaa tccgttctag ctggtatttg
    11941 aaaaatcaaa ctaatattca ataaaaatca aagaacaaac taggaagcta gccgcaggtt
    12001 gctcaaaaca ctgttttgag gttgtagata gagctgacgt agtttgaaga gattttcgaa
    12061 gagtataagc tgcaagatga atgattttct tgtattgacg ttgttgttga caaaaagtag
    12121 cggataaatg aaatccattc cattatcata gatgataggc tggtaggaaa ttttcaaata
    12181 gcatacagga aatagatgta tggagttctg gtagtagaaa gggagagaga tgaacatttt
    12241 agttgcagat gacgaggaaa tgattagaga aggaattgca gcatttctga cagaagaggg
    12301 ttatcatgtc attatggcta aggatggaca agaggtcttg gaaaaatttc aagatctccc
    12361 tatccatctc atggtactgg atttaatgat gcctaggaag agtggttttg aagtgttaaa
    12421 agaaatcaat caaaagcacg atattcctgt catcgtcttg agtgctctgg gagatgaaac
    12481 tactcagtca caggtatttg atctctatgc tgatgatcat gtgacaaaac ctttttcttt
    12541 ggtactgctt gtcaagcgta ttaaggcgct tatcagacgt tactacgtca tagaggatct
    12601 ttggcgatat caggatgtaa cagtggattt tacctcttac aaagcacatt ataaaaatga
    12661 agaaattgat ctcaaaccaa aggaattact ggtactaaag tgtttgattc agcataaaaa
    12721 tcaagtttta agtagagagc agatattgga agaaatttca aaagatgtag ctgatttacc
    12781 ttgtgatagg gtcgttgatg tctatattcg tactcttcgc aaaaaattag ctttagattg
    12841 tatcgtgact gtgaaaaatg ttgggtataa gattagctta tgataaaaaa tcctaaatta
    12901 ttaaccaagt cttttttaag aagttttgca attctaggtg gtgttggtct agtcattcat
    12961 atagctattt atttgacctt tcctttttat tatattcaac tggaggggga aaagtttaat
    13021 gagagcgcaa gagtgtttac ggagtattta aagactaaga catctgatga aattccaagc
    13081 ttactccagt cttattcaaa gtccttgacc atatctgctc accttaaaag agatattgta
    13141 gataagcggc tccctcttgt gcatgacttg gatattaaag atggaaagct atcaaattat
    13201 atcgtgatgt tagatatgtc tgttagtaca gcagatggta aacaggtaac cgtgcaattt
    13261 gttcacgggg tagatgtcta caaagaagca aagaatattt tgcttttgta tctcccatat
    13321 acatttttgg ttacaattgc tttttccttt gttttttctt atttttatac taaacgcttg
    13381 ctcaatcctc ttttttacat ttcagaagtg actagtaaaa tgcaagattt ggatgacaat
    13441 attcgttttg atgaaagtag gaaagatgaa gttggtgaag ttggaaaaca gattaatggt
    13501 atgtatgagc acttgttgaa ggttattcat gagttggaaa gtcgtaatga gcaaattgta
    13561 aaattgcaaa atcaaaaggt ttcctttgtc cgtggagcat cacatgagtt gaaaacccct
    13621 ttagccagtc ttagaattat cctagagaat atgcagcata atattggaga ttacaaagat
    13681 catccaaaat atattgcaaa gagtataaat aagattgacc agatgagcca cttattggaa
    13741 gaagtactgg agtcttctaa attccaagag tggacagagt gtcgtgagac cttgactgtt
    13801 aagccagttt tagtagatat tttatcacgt tatcaagaat tagctcattc aataggtgtt
    13861 acaattgaaa atcaattgac agatgctacc agggtcgtca tgagtcttag ggcattggat
    13921 aaggttttga caaacctgat tagtaatgca attaaatatt cagataaaaa tgggcgtgta
    13981 atcatatccg agcaagatgg ctatctctct atcaaaaata catgtgcgcc tctaagtgac
    14041 caagaactag aacatttatt tgatatattc tatcattctc aaatcgtgac agataaggat
    14101 gaaagttccg gtttgggtct ttacattgtg agtaatattt tagaaagcta tcaaatggac
    14161 tatagttttc tcccttatga acacggtatg gaatttaaga ttagcttgta gacagattag
    14221 ttttttatta aagttcacat agggttaaca taagtgtatt attctttata tagataaaaa
    14281 tatagataaa agaaaggata ctaatatggt attagcgatt attttagtaa cattccttat
    14341 tcgattgatt tttttaaagc gttcgataga gaatgagaaa cgaatcctta gcaatggcgg
    14401 gaaagaattt ggagttgaga atacaaaacg attaacgttg gctcatattg ttttttatct
    14461 ctcttgcctc gttgaagcaa tggtgcacaa gacaattttt gatggcatgg gcatggttgg
    14521 tttagtcttg cttatttttt ctatgctgat gttgatgttg gtgattcact tgttgggaga
    14581 tatttggaca gtgaagctta tgcttgtcaa taatcacaaa tatgtagatc atatcttgtt
    14641 taggacagta aaacatccta attacttttt aaatattctt cctgagttga ttggcttgac
    14701 cttgttgagt catgcttatg tgacttttgt tttagttttt ccagtttatg cagttatttt
    14761 gtatcgacga atagctgaag aggaaaagct attacatgaa gttataatcc caaatggaag
    14821 cataaagaga taaatacaaa attcgattta tatacagttc atattgaagt gatatagtaa
    14881 ggttaaagaa aaaatataga aggaaataaa catgtttgca tcaaaaagcg aaagaaaagt
    14941 acattattca attcgtaaat ttagtattgg agtagctagt gtagtagttg ctagcttgtt
    15001 cttaggagga gtagttcatg cagaagaggt tggtggtagg aataccccca cggttacatc
    15061 tagtgggcaa gatatatcga agaagtatgc tgatgaagtc gagtcgcatc taaaaaaaat
    15121 attgagtgag atccaaacac aattagatag aaaacgacat accaaaactg tagcgctaat
    15181 taacgagttg caaaacatta agaaaacgta tttgtataat ttaaatgttt taaaagagaa
    15241 gtctgagttg ccgtcaaaaa taaaagcaaa gttagaagta gcttttgatc agtttaaaaa
    15301 agatacattg aaaccaggag aaaaggtagc agaagctgag aagaaagttg cagaagctaa
    15361 gaaaaaagcc gaggatcaaa aagaacaaga tcgccgtaac tacccaacca atacttacaa
    15421 aacgcttgaa cttgaaattg ctgagtccga tgtgaaagtt aaagaagcgg agcttgaact
    15481 agtaaaagag gaagctaagg aatctcgaaa cgaggaaaaa gttaagcaag caaaagcgaa
    15541 agttgagagt gaaaaagcag aggctataag gttagaagaa atcaagacag atcgtgaaga
    15601 agctaaacga aaagcagatg ctaagttgaa ggaagctgtt gaaaacaatg cagcgacttc
    15661 agagcaaggt gaaccaaaga ggcgggtaaa acgaggagtt cttggagagc cagcaacacc
    15721 tgataaaaaa gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc
    15781 aagcccatcc ctgaaaccag aaaaaaaggt tgcagaagct gagaagaagg ttgcagaagc
    15841 tgagaaaaaa gccaaggatc aaaaagaaga agatcgccgt aactacccaa ccaatactta
    15901 caaaacgctt gaacttgaaa ttgctgagtc cgatgtgaaa gttaaagaag cggagcttga
    15961 actagtaaaa gaggaagcta aggaatctcg aaacgaggaa aaagttaagc aagcaaaagc
    16021 gaaagttgag agtaaaaaag ctgaggctac aaggttagaa aaaatcaaga cagatcgtaa
    16081 aaaagcagaa gaagctaaac gaaaagcagc agaagaagat aaagttaaag aaaaaccagc
    16141 tgaacaacca caaccagcgc cggctccaaa accagaaaaa ccagctccag ctccaaaacc
    16201 agagaatcca gctgaacaac caaaagcaga aaaaccagct gatcaacaag ctgaagaaga
    16261 ctatgctcgt agatcagaag aagaatataa tcgcttgact caacagcaac cgccaaaaac
    16321 tgaaaaacca gcacaaccat ctactccaaa aacaggctgg aaacaagaaa acggtatgtg
    16381 gtacttctac aatactgatg gttcaatggc gacaggatgg ctccaaaaca atggctcatg
    16441 gtactatcta aacgctaatg gttcaatggc aacaggatgg ctccaataca atggttcatg
    16501 gtactaccta aacgctaatg gtgatatggc gacaggatgg ctccaaaaca atggttcatg
    16561 gtactaccta aacgctaatg gttcaatggc gacaggatgg ctccaataca atggctcatg
    16621 gtactaccta aacgctaatg gtgatatggc gacaggatgg ctccaaaaca atggctcatg
    16681 gtactaccta aacgctaatg gttcaatggc gacaggatgg ctccaaaaca atggctcatg
    16741 gtactaccta aacgctaatg gttcaatggc gacagattgg gtgaaagatg gagatacctg
    16801 gtactatctt gaagcatcag gtgctatgaa agcaagccaa tggttcaaag tatcagataa
    16861 atggtactat gtcaatggct caggtgccct tgcagtcaac acaactgtag atagctatag
    16921 agtcaatgcc aatggtgaat gggtaaacta aacctaatat aactagttaa tactgacttc
    16981 ctgtaagaac tctttaaagt attccctaca aataccatat cctttcagta gataatatac
    17041 ccttgtagga agtttagata aaaaaataac tctgtaatct ctagccggat ttatagcgct
    17101 agagactacg gagttttttt gactctttgt caactgtagt gggttgaagt cagctaagct
    17161 tgagaaagga caaatttcgt cctttctttt ttgatgttca gagcgatgaa aatccgtttt
    17221 ttgaagtttt caaagttccg aaaaccaaag gcattgcgct tgataagttt gatgagatta
    17281 ttggtcgctt ccaatttggc gtttgaatag ggtagttgaa gggcgttgac gattttctct
    17341 ttgtccttta gaaaggtttt aaagacagtc tgaaaaagag gatgaacctg ctttagattg
    17401 tcctcaatga gtccgaaaaa tttctccggt tccttattct gaaagtgaaa cagcaagagt
    17461 tgatagagct gatagtgatg tttcaagtct tgtgaatagc tcaaaagctt gtctaaaatc
    17521 tctttattgg ttaaatgcat acgaaaagta gggcgataaa aatgtttatc gctgagttta
    17581 cgactatcct gttgtatgag cttccagtag cgcttgatag ccttgtattc atgggatttt
    17641 cgatgaaact gattcatgat ttgaacacgc acacgactca tagcacggct aagatgttgt
    17701 acaatatgaa agcgatcaag aacgatttta gcattcggga gtgaaacagt ctaggagact
    17761 gtttcagcct gagcctagaa atttgaaagc gaagctgttt agctaagtca tagtaagggc
    17821 taaacatatc catagtaatg attttgacgc gacatcggac ggctctatca tatttaagaa
    17881 agtgatctcg gatgacagct tgtgttctac cttcaagaac agtgatgata ttgagattgt
    17941 taaaatcttg cgcaatgaag ctcatctttc cctttgtaaa agcatactca tcccaagaca
    18001 taatctcagg aagacgcgaa aaatcatgtt taaagtgaaa atcattgagc ttacgaataa
    18061 cagttgaagt tgagatggaa agttgatggg caatatcagt catagaaatc ttttcaatta
    18121 acttttgagc aatcttttgg ttgatgatac gagggatttg gtgattcttc ttgacgatag
    18181 aagtttcagc gaccatcatt tttgaacagt gatagcactt gaatcgacgc tttctaagga
    18241 gaattctagt aggcatacca gtcgtttcaa gataaggaat tttagaaggt ttttgaaagt
    18301 ca
//
LOCUS       DAVHIU010000032        17471 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVHIU010000032 DAVHIU010000000
VERSION     DAVHIU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.034"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            15..489
                     /locus_tag="VVN06_000924"
                     /pseudo
     CDS             15..489
                     /locus_tag="VVN06_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            771..1018
                     /locus_tag="VVN06_000925"
                     /pseudo
     CDS             771..1018
                     /locus_tag="VVN06_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            1123..1425
                     /locus_tag="VVN06_000926"
                     /pseudo
     CDS             1123..1425
                     /locus_tag="VVN06_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1581..2810
                     /locus_tag="VVN06_000927"
     CDS             1581..2810
                     /locus_tag="VVN06_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5562501.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2830..5526
                     /gene="ppc"
                     /locus_tag="VVN06_000928"
     CDS             2830..5526
                     /gene="ppc"
                     /locus_tag="VVN06_000928"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU5562502.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITHKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5609..5826
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5939..6943
                     /locus_tag="VVN06_000929"
     CDS             5939..6943
                     /locus_tag="VVN06_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5562503.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7012..7878
                     /locus_tag="VVN06_000930"
     CDS             7012..7878
                     /locus_tag="VVN06_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562504.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7875..8633
                     /locus_tag="VVN06_000931"
     CDS             7875..8633
                     /locus_tag="VVN06_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562505.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            8723..10483
                     /gene="dnaG"
                     /locus_tag="VVN06_000932"
     CDS             8723..10483
                     /gene="dnaG"
                     /locus_tag="VVN06_000932"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5562506.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYQAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10486..11595
                     /gene="rpoD"
                     /locus_tag="VVN06_000933"
     CDS             10486..11595
                     /gene="rpoD"
                     /locus_tag="VVN06_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU5562507.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11609..11938
                     /locus_tag="VVN06_000934"
     CDS             11609..11938
                     /locus_tag="VVN06_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5562508.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12053..13096
                     /gene="cpoA"
                     /locus_tag="VVN06_000935"
     CDS             12053..13096
                     /gene="cpoA"
                     /locus_tag="VVN06_000935"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU5562509.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13112..14437
                     /locus_tag="VVN06_000936"
     CDS             13112..14437
                     /locus_tag="VVN06_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5562510.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14468..14644
                     /locus_tag="VVN06_000937"
     CDS             14468..14644
                     /locus_tag="VVN06_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5562511.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14703..14837
                     /locus_tag="VVN06_000938"
     CDS             14703..14837
                     /locus_tag="VVN06_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU5562512.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14901..16211
                     /gene="obgE"
                     /locus_tag="VVN06_000939"
     CDS             14901..16211
                     /gene="obgE"
                     /locus_tag="VVN06_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5562513.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16221..16379
                     /locus_tag="VVN06_000940"
     CDS             16221..16379
                     /locus_tag="VVN06_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562514.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(16431..17471)
                     /locus_tag="VVN06_000941"
                     /pseudo
     CDS             complement(16431..17471)
                     /locus_tag="VVN06_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5397 a         3000 c         3892 g         5182 t
ORIGIN      
        1 attgggaacc gtacatgaaa aagtacacgg aagtttttca aagtcaattt cctaatctaa
       61 acattgatta cttgctcctg gacactgagc agattgatct tgatagctat ctagatgctg
      121 acataattat catcggtggt ggaaatacgg aaaaatatat tgctaactta tgttaatcag
      181 gagttcaaaa gttatatcga tcatatgctt aataaagagg caaaaattat aggattttct
      241 gcaggagccc tactattagg agaaaaagtc tatgtctcac ctaatgataa ttcagatcat
      301 cagataaaga taaaaaatgg attaggactc tttagtcagt ttttaattag tgtccattat
      361 gattcctgga atgataaagc aaataaggat agagctgaag aactcgttaa tgttcccata
      421 attccactaa atgatcattc ttgtcttgta ttggataaac ttgaaaacat catagagaaa
      481 attgactaac tttcataatg taaaaatgga tctaggaaat aaaagtgtag acgaactgga
      541 taggttacta gtgaaaactg caaaaagtgt tgtaaagtga atcggagtgt gcttgatgga
      601 ttttagtagt aagatagcca taaataaagg ctggtcagat gataaaaaat attgtgtgac
      661 agatcaaaat cagtaaaaat atttcttgcg tgtttctgat aagtcgaagt tagattataa
      721 aaaaatttga atttgatatg atggagaaag tagcttctct tggagttccg atgtgtaaac
      781 caattagcat tgaactctgc gatgacgaag tacattcttt acacgaatgg atagacggaa
      841 gagatgcaat agatagcatt ttagcttatt cagaaaacca acaatacaca tatggtgtag
      901 aagcaggaaa gatacttaga aagattcata caattcctgc tacagaagtt tgtgaagatt
      961 gggaaatttt tttaatctaa aaattgatga taaaatctcc aacgaaatga tatggtaagt
     1021 ccgaaacgct cagtgtgtaa gcttgttgct aaaagcgtaa aaaccttgga acgaaaggaa
     1081 taatagactt cctgcgaaac aaaaatacaa tacaataaaa ctatggatga tgaagcaagt
     1141 aaacaattaa gcgatagccg tttcaagatc cttgtaggtg ttcagcgcac gacttttgaa
     1201 gagatgttag ctgtgttaaa aacagcttat caacgtaaac gcgcaaaagg tggacgaaaa
     1261 agcaaattaa gcctagacga tctccttatg gtaactattc aatacatgcg agaatagagc
     1321 acttatgaac aaattgcggc tgattttggc attcacgaaa gcaacttaat ccgtcggagt
     1381 caatgggttg aagcaactct tattcaaaat ggttttacga tttcaaattc tgccttaatt
     1441 ctgtaaaaac agtaaaattc gaaggattgt aaggtaagag tttttttctt tctgaaaaaa
     1501 tggtataata gcaatcaaaa ctagaaaata aaacggaatt tggaacagat ttgtctgtat
     1561 cctagtagag tggtgatact atgaagatta gtaagaggca cttattaaat tattccatct
     1621 tgattcccta cttgctttta tctattttgg gcttgattgt ggtctattcg accaccagtg
     1681 ctattttaat tgaagaaggc aagagcgcct tgcagttggt tcgaaaccaa ggaatctttt
     1741 ggattgttag tttgatactg attgccttaa tttataaatt gagactagat tttttgagaa
     1801 atgagcgact aatcatttta gttatattaa tagaaatgct tttattgttc ttggctcgtt
     1861 ttattggtat ttcagtaaac ggggcatacg gttggatttc ggttgcagga gtaactattc
     1921 agccagctga gtacttaaaa atcattatta tttggtattt agctcaccga ttctccaaac
     1981 agcaagaaga aatagctact tatgattttc aagttttgac tcaaaatcaa tggcttcccc
     2041 gtgcttttaa tgattggcga ttcgttctcc tagttctgat tggaagtttg ggaattttcc
     2101 ctgatttagg aaatgcgact attttagtct tggtttcctt gattatgtat acagttagtg
     2161 gaatcgctta tcgctggttt tcaaccattc tggcgctcgt atctgccact tctgtctttg
     2221 tcttgaccac tatcagccta atcggtgttg agaccttttc aaaaattcca gtatttggct
     2281 atgtagccaa gcgctttagt gcctttttta atccttttgc cgatcgtgct gatgcaggtc
     2341 accagttagc taattcttat tttgccatgg tcaatggtgg ttggtttggt ctaggtcttg
     2401 gaaactcgat tgaaaaacga ggttatttgc cagaagctca tacagacttt gtcttttcta
     2461 tcgtgattga agaatttggc tttgttggtg ccagtcttat tttagctctc ttgtttttca
     2521 tgattttgcg gattatcttg gtcggtatcc gagcggagaa tcctttcaat gccatggttg
     2581 cactcggtgt cggagggatg atgttggttc aggtatttgt caatatcgga gggatttcgg
     2641 gcttgattcc atctacagga gtgactttcc ctttcttatc ccagggtgga aatagtcttc
     2701 tagtcttatc agtggcagta gcctttgtct taaatattga tgccagtgaa aaacgcgcta
     2761 agttgtaccg agaattggaa aatcaaccaa tgaaccttct gttgaagtag gataaagaaa
     2821 ggatagttta tgtctcttca aaaattagaa aattatagta ataaaagtgt tgtgcaagaa
     2881 gaagtcttga ttctaacaga attactggaa gatattacta aaaatatgct tgccccagag
     2941 acctttgaaa aaataataca gttgaaagaa ttatcaacgc aggaagatta tcaaggtcta
     3001 aaccgtctag tgactagctt atcaaatgat gaaatggtct atatttcacg ctatttctct
     3061 atcttacctc ttttgattaa tatttcagag gatgtggatt tagcttatga aatcaatcat
     3121 caaaataata ttgatcagga ctatttaggt aaattatcta caacgattaa attggtagca
     3181 gaaaaggaaa atgccgttga gatcctagaa cacttgaatg ttgtccctgt tttgacagcc
     3241 catccaacac aagtgcaacg caaaagtatg ttggatttaa caaatcatat tcatagtctt
     3301 ttgcgtaaat accgtgatgt taagttgggg ttgatcaata aagataaatg gtacaatgat
     3361 ttgcgtcgtt acatcgaaat tatcatgcag acagacatga ttcgtgagaa aaaattaaaa
     3421 gtgactaacg aaatcacgaa tgctatggaa tattataaca gctccttttt gaaagctgta
     3481 cctcatttga cgacggagta taagcgctta gcgcaagcgc atggtctgaa tttaaaacag
     3541 gctaaaccaa tcaccatggg tatgtggata ggtggtgacc gtgatggaaa tccatttgtt
     3601 acagcaaaga ccttgaagca gtctgcactc actcagtgtg aagtcatcat gaactactat
     3661 gataaaaaga tttaccaact ttatcgtgaa ttttctcttt caactagcat tgtcaacgtc
     3721 agcaagcaag tcagagaaat ggctcgtcaa tccaaggata actcgattta ccgcgaaaaa
     3781 gagctttacc gtcgtgcctt gtttgatatt caatcaaaaa ttcaggcaac taaaacctat
     3841 ctgattgagg atgaagaagt tgggactcgt tatgaaaccg ccaatgattt ctacaaggat
     3901 ttgattgcca ttcgagattc tctactagaa aataagggcg agtccttgat ttcaggtgat
     3961 tttgtggaat tattgcaggc agtagagata tttggttttt acttagcatc aattgatatg
     4021 cgacaagact ctagcgtcta tgaagcctgt gtggcagaac tcttgaaatc agcaggaatt
     4081 cattctcgtt atagcgagtt gagcgaagaa gaaaagtgtg accttctctt gaaagaatta
     4141 gaagaagatc cccgaattct ttctgcgact cacgcagaaa aatcagaatt attagcaaaa
     4201 gaattagcta tttttaagac ggctcgtgtt ttgaaagata agttgggaga tgatgtcatc
     4261 cgtcagacca tcatttcaca tgcaaccagc ctttctgata tgctagaatt agctattctg
     4321 ttaaaagaag taggactggt ggatacggaa agggcgcgtg ttcagattgt tccccttttt
     4381 gaaacaattg aagacttgga tcattcagag gaaacaatga gaaaatatct ttctcttagc
     4441 cttgccaaaa aatggattga ctcacgaaat aactaccaag aaatcatgct tggctactct
     4501 gacagtaata aagatggcgg ttacttgtca tcatgttgga ccctctacaa ggctcaacaa
     4561 caattgactg ctattggaga tgaatttggc gttaaggtta ccttcttcca tggtcgtggt
     4621 ggtactgtcg gtcgtggtgg tgggccaacc tatgaagcca ttacatctca accgctcaag
     4681 tctatcaagg atcgtatccg tttgacggag cagggtgaag taattgggaa taaatacggt
     4741 aacaaagacg ccgcttacta taaccttgaa atgctagtat ctgcagctat taaccgtatg
     4801 attactcata agaagagtga taccaatacc ccaaatcgtt atgaagccat tatggatcaa
     4861 gtagtggacc gtagttacga tgtctaccgt gatttggtct ttggtaatga gcatttctat
     4921 gattatttct tcgagtcaag tccaatcaag gctatttcaa gttttaatat tggttctcgt
     4981 ccagccgctc gtaagactat tactgaaatc ggtggtttgc gtgccatccc ttgggtattc
     5041 tcatggtcac agagtcgtgt tatgttccct ggatggtacg gggttggttc aagcttcaag
     5101 gaatttatca ataaaaatcc agagaatatt gctatcttac gagatatgta ccaaaattgg
     5161 cctttcttcc aatcgcttct ttcaaatgtt gatatggttt tgtcaaaatc aaatatgaat
     5221 attgcttttg aatatgctaa actttgtgaa gacgagcaag ttaaggccat ctatgagact
     5281 attttaaatg aatggcaagt tactaagaac gttatcttgg ctattgaagg acatgacgaa
     5341 ctcttagctg acaatccata tctaaaagct agtctggatt accgtatgcc ttactttaat
     5401 attctcaact atattcagtt agagttgatt aaacgccaac gtcgtggaga attgtccagt
     5461 gatcaagaac gattgattca tatcaccatc aacggaattg cgacaggatt gcgtaattca
     5521 ggttgataat tttcaagagt gaatgctaaa agtgaatatc aaaaaaattc taatagacta
     5581 ttgacaagta gtttaaaaat gatataattt aaccattcag aaaagtaatc atacaaactt
     5641 tttagagagt ctgtggtagc tgaaaacaga taagtggcaa tgatgaaaat tgggctgaat
     5701 gctatttaga atttgaaatt ataaaaattc ggtaagcaca ccttacagtg catctcgtta
     5761 ttgcgagact gagcgatagg gaaattccct ataattgagg tggtaccgcg catcgacgtc
     5821 ctcacacaag ttttttgtgt gaggattttt ttgatggagg ttagtatgga aagaaaacaa
     5881 tggcgtcgct tgtttagata agtgaaatat gttaaaggaa ataaaaagga gaaacagaat
     5941 gaaaaataaa cgtttaattg gaattattgc tgcattagca gtcttagtag caggaagctt
     6001 gatttattct tcaatgaata aatcagaagc tcagaataat aaggatgaga agaaaataac
     6061 caagattggt gtgcttcaat ttgtgagcca tccatccctt gatttgattt ataaagggat
     6121 ccaagatgga cttgcagaag aaggatataa agatgatcaa gttaaaattg attttatgaa
     6181 ctcagaaggt gaccaaagta aggttgcgac aatgagtaaa caattggttg caaatgggaa
     6241 tgaccttgtg gttggtatcg caacaccagc agcccaaggg ttggctagtg caacaaaaga
     6301 cctaccggtt atcatggccg ctattacaga cccaattggt gctaacttgg ttaaagattt
     6361 gaaaaaacca ggtggcaacg ttacaggggt atctgaccac aatccagctc aacaacaagt
     6421 tgaactcatc aaggctctga caccgaatgt gaaaacaatc ggagctcttt actcaagtag
     6481 cgaagacaat tcaaaaacac aggtcgaaga atttaaggct tatgctgaaa aagcaggtct
     6541 gacagtggaa acatttgcag ttccttcaac aaatgaaatt gcctcaactg tcactgttat
     6601 gactagcaag gtagatgcta tttgggttcc aattgataac accattgcat caggatttcc
     6661 aacggttgtc tctagcaatc aaagttctaa gaaaccaatt tatcccagtg cgacagctat
     6721 ggtagaagta ggtggtttgg catcagttgt aattgaccaa catgaccttg gtgtggcaac
     6781 aggtaaaatg attgtgcaag tcttgaaagg tgcaaaacca gccgataccc cagtcaatgt
     6841 cttttcaact ggtaagtcag tcatcaataa aaaaatagca caagaactag gtattactat
     6901 tcctgagtct gttctcaaag aagcaggaca agtcatcgaa taaataaggt agggaggagg
     6961 agactcttcc cattttttaa aaagatgaat atagaaagga ttaaataaaa tatgattgtt
     7021 tccattattt ctcaaggatt tgtctgggct attctaggtc tgggaatctt tatgacattt
     7081 aggattttaa actttccaga tatgacgaca gaaggttcct tccctcttgg gggagctgtt
     7141 gctgtcactt tgataaccaa aggcgtgaac ccatttttag cgacacttgt tgctgtagga
     7201 gcaggttgtt tggctggaat ggcagcaggc cttctttata caaaagggaa gatcccaacc
     7261 ttgctctcag ggattttggt gatgacttct tgtcactcaa tcatgctctt gattatggga
     7321 cgtgcgaatt taggcctgct tggaaccaag caaattcagg atgttttgcc ttttgattcg
     7381 gatttgaatc aactcttgac aggtctcatc tttgtgagta ttgttattgc tctcatgctc
     7441 tttttcttgg acactaaact cggacaagcc tatattgcta caggggataa tcctgatatg
     7501 gctagaagtt tcgggattca tactggacac atggagctca tgggcttggt cttatcaaat
     7561 ggtgtgattg cccttgcagg tgccctcatt gctcagcaag aaggttatgc cgatgtgtct
     7621 cgagggatcg gggttatcgt tgtggggctt gcaagtttga ttattggaga agttattttc
     7681 aagagtttga gcttggcaga gcgtttggtt actatcgttg taggttctat cgcttatcaa
     7741 tttttagtgt gggcagttat cgcacttggc tttaatacaa gttaccttcg tttatacagt
     7801 gccttgattt tagcagtctg cctcatgatt ccaacattta agcaaacaat cttgaaagga
     7861 gccaagttaa gcaaatgaca gcaattgtag aattaaaaaa tgcaaccaaa atcgttaaaa
     7921 atggctttga tgaagaaaag attattttaa atgatgtttc cttagaaatt tttgaacagg
     7981 actttatcac gattttgggt ggaaatggtg ctggtaaatc aaccctcttt aacactattg
     8041 caggaaccat atcactaacc agtggaacta tccgtatttt aggtgaggat gttaccaagt
     8101 tttcaccaga gaagcgcgct aagtatctgt ctcgagtctt ccaagatcca aagatgggga
     8161 cagctcctcg tatgacagtg gcagagaatc tcctgattgc caagtttcgt ggtgaaaagc
     8221 gtggactatt tccgagacgt ctggctagct ataaggataa gtttcaggcg accattgaaa
     8281 aagtgggaaa cggtcttgag aaacacttga atacaccgat tgagttctta tctggtggac
     8341 aaagacaggc tttgagtctc ttgatggcaa ccttgaagcg acctgaatta ctcttgttag
     8401 acgagcatac tgctgccctg gatccaaaga ctagtgttgc tttgatggaa ttgacagatg
     8461 aatttgttaa gaaagatcaa ttgacagccc ttatgattac ccatcatatg gaagatgctc
     8521 tcaaatatgg caatcgcttg attgtcatga aagaaggacg ggttatccaa gatttgaaac
     8581 aagaagaaaa agcaaaaatg aaaatctctg attattatca actctttgaa taaagtaggg
     8641 aaaatgagtg aaatggttta ctttttttct gaaataaagt atactatata aagtaaacta
     8701 tgataacatg gaggtattgt gtatggttga caaacaagtc attgaagaaa tcaaaaacaa
     8761 tgccaacatt gtggaagtca taggagatgt gatttcttta caaaaggcag gacggaacta
     8821 tctagggctc tgtccttttc atggtgaaaa aacaccttct ttcaacgttg tagaggacaa
     8881 gcagttttac cactgttttg gttgtggtcg ctcaggtgat gtctttaaat tcatcgagga
     8941 gtaccaaggg gttaccttta tggaggctgt ccaaatctta ggtcagcgtg tcgggattga
     9001 ggttgaaaaa ccgctttata gtgaacagaa gccagcctcg cctcaccaag ctctttatga
     9061 tatgcacgaa gatgcggcta aattttacca tgctattctc atgacaacga ctatgggcga
     9121 agaggccaga aattaccttt atcagcgggg tttgacagat gaagtgctta aacatttttg
     9181 gattggttta gcacctccag aacgaaacta tctctatcaa cgtttgtctg atcagtatcg
     9241 tgaagaagat ttactggatt caggcctgtt ttatctctcg gatgccaatc aatttgtaga
     9301 cacctttcac aatcgcatta tgtttcccct gacaaatgac caaggaaagg tcattgcctt
     9361 ctcaggtcgt atctggcaaa aaacagattc acaaacttct aagtataaaa acagccgatc
     9421 gactgcaatt tttaacaaaa gttacgaatt atatcatatg gatagggcaa aaaaatcttc
     9481 tggaaaaact agtgagattt acctgatgga aggattcatg gatgttattg cagcctatca
     9541 ggctggaatc gaaaatgctg tggcgtcgat gggaacggcc ttgagtcgag agcatgttga
     9601 gcatctgaaa aggttaacca agaaattggt tcttgtttac gatggagata aggctgggca
     9661 agccgcgata ttgaaagcat tggatgaaat tggtgatatg cctgtgcaaa tcgttagcat
     9721 gcctgataac ttggatcctg atgagtatct acaaaaaaat ggtccagaag acttggccta
     9781 tctattaacg aaaactcgta ttagtccgat tgagttctac attcatcagt acaaacctga
     9841 aaacagtgaa aatctgcagg ctcagattga gtttattgaa aaaatagctc ccttgattgt
     9901 tcaagaaaag tccatcgctg ctcaaaacag ctatattcat attttagctg acagtctggc
     9961 gtcctttgat tatgcccaga ttgagcagat tgttaatgag agtcgtcagg tgcaaaggca
    10021 gaatcgcatg gaaggaattt ccagaccgac gccaatcacc atgcctatca ccaagcagtt
    10081 atcggctatt atgagggcag aagcccatct actctatcgg atgatggaat cccctcttgt
    10141 tttgaacgat taccgtttgc gagaagactt tgcatttgct acacctgaat ttcaggtctt
    10201 atatgacttg cttggccagt atggaaatct tcctccagaa gttttagcag agcagacaga
    10261 ggaagttgaa agagcttggt accaagtttt agctcaggat ttgcctgctg agatatcgcc
    10321 gcaggaactt agtgaagtag agatgactcg aaacaaggct ctcttgaatc aggacaatat
    10381 gagaatcaaa aagaaggtgc aggaagctag ccatgtagga gatacagata cagccctaga
    10441 agaattggaa cgtttaattt cccaaaagag aagaatggag taataatggc aacaaaacaa
    10501 aaagaagtaa caacatttga cgtacaggta gcagaattta tccgtaatca taagcaaaaa
    10561 gggacagcaa cagatgatga aatcaatgca agtctggtta ttccttttac cttggacgct
    10621 gatgggattg aagatctctt gcaacggatt caggatgcag gcatttctat cacagataac
    10681 gaaggaaatc caagtgcgcg tgttcttagc aatgaagaag aaccagaact cagcgatgag
    10741 gacttgattg ggtcaacttc tgctaaggtc aatgaccctg tccgtatgta cttgaaagaa
    10801 ataggggtcg ttcctctctt gaccaatgaa gaggagaaag agttggcact ggctgttgaa
    10861 gctggcgata tcgaagctaa acaacgtctt gcggaagcca atcttcgttt ggttgtttcc
    10921 attgccaaac gctatgtcgg tcgtggcatg cagttccttg acttgattca agaaggaaat
    10981 atgggcttga tgaaggcggt tgacaagttt gactattcta aagggttcaa gttttcaact
    11041 tatgcaactt ggtggattcg tcaggctatc actcgtgcta ttgcggatca agctcgtacc
    11101 atccgtatcc cagttcacat ggttgaaact atcaataaat tggttcgtga acagcggaat
    11161 ctccttcaag aattggggca agatccgaca ccagaacaga ttgctgaacg aatggatatg
    11221 acacctgata aggttcgtga aatcttgaag attgcccaag aaccagtatc tcttgaaact
    11281 cctatcggtg aagaggacga tagccacctt ggagacttta tcgaagatga agtgattgaa
    11341 aatccagtgg attatacgac tcgtatcgtc ttgcgtgagc aattggatga aatcttagat
    11401 actcttacag accgtgaaga aaatgttctg cgtctacgtt ttggactaga tgatggaaaa
    11461 atgcgcacac ttgaagatgt ggggaaagtc tttaacgtaa ctcgtgagcg tatccgtcag
    11521 attgaagcaa aggctttgag aaaactacgc caaccaagtc gcagcaaacc gcttcgtgat
    11581 tttattgaag actaagagtg aggaaaatat ggcttataca gaagagcaaa ttgaaaacat
    11641 caaaacacgg attttaacag ccttggaaga agtcatcgac cctgagttgg gaatcgatat
    11701 tgtcaatctt ggtttgatct atgagattcg ttttgacggt gacacagggc aaacagagat
    11761 tgacatgact ttgacaacta tgggttgtcc cttagcagac cttttgacag accagattta
    11821 tgatgctatg atagaggttc cagaagtaac ggatactgag gttaaattag tctggtatcc
    11881 agcctggaca gttgaaaaaa tgagtcgcta tgcccgcatt gccctaggca ttaagtaaac
    11941 atataaaagc atgtgagaga ctgttggaaa agcgaggaaa tttcccctct tttcctctag
    12001 tctctccttt cttttgctga ttttattcaa agaaaatgat ataatagtag ttatggagaa
    12061 aaagaaatta cgcatcaata tgttgagttc aagtgagaaa gtagcaggac agggagtttc
    12121 aggtgcttac cgtgaattag ttcgtcttct tcaccgtgct gccaaggacc aattgattgt
    12181 tacagaaaat cttccgatcg aggcagatgt gactcacttt catacgattg attttcccta
    12241 ttatttatca accttccaaa agaaacgctc agggagaaag attggctatg tgcatttctt
    12301 gccagctaca cttgagggaa gtttgaaaat tccatttttc ttaaagggaa ttgtgaaacg
    12361 ctatgtattt tctttttaca accggatgga gcacttggtt gtggtcaatc ctatgtttat
    12421 tgaggatttg gtagcagctg gtattccacg tgaaaaagtg acctatattc ctaactttgt
    12481 caacaaggaa aaatggcatc ctctaccaca agaagaggta gtcagactgc gcacagatct
    12541 tggtcttagt gacaatcagt ttatcgtagt aggtgctggg caagttcaga aacgtaaagg
    12601 gattgatgac tttatccgtc tggctgagga attgcctcag attaccttta tctgggctgg
    12661 tggcttctct tttggtggta tgacagatgg ttatgaacac tataagaaaa ttatggaaaa
    12721 tccccctaaa aatttgattt ttccaggcat tgtatcgcca gagcggatgc gcgaattgta
    12781 tgctctagcg gatcttttct tgttgcctag ttacaatgag ctctttccta tgactatttt
    12841 agaagctgcg agttgtgagg ctcctattat gttgcgtgat ttagatctct ataaggtgat
    12901 tttggaggga aattatcggg cgacagcggg tagagaagag atgaaagagg ctattttgga
    12961 atatcaagca aatcctgctg ccttaaaaga tctcaaagaa aaggctaaga atatttccag
    13021 agagtattct gaagagcatc tgttacaaat ctggttggac ttttatgaga aacaagccgc
    13081 tttagggaga aagtaaaaag tgaggtaatc tatgcgaatt ggtttattta cagataccta
    13141 ttttcctcag gtttctggtg ttgcgaccag tattcgaacc ttgaaaacag aacttgaaaa
    13201 gcagggacat gctgttttta tctttacgac gacagataag gatgtcaatc gctacgaaga
    13261 ttggcaaatt atccgcattc caagtgttcc tttctttgct tttaaggatc gtcgctttgc
    13321 ctaccgaggt tttagcaagg cacttgaaat tgctaaacag tatcagctag atattatcca
    13381 tactcagaca gaattttctc ttggcctgtt ggggatttgg attgcgcgtg aattgaaaat
    13441 tccagtcatc catacctatc acacccagta tgaagactat gtccattata ttgctaaggg
    13501 gatgttgatc cgaccgagta tggtcaagta tctggttaga ggtttcctgc atgatgtgga
    13561 tggggttatt tgccctagtg agattgtccg tgacttgcta tctgattata aggtcaaggt
    13621 tgaaaaacgg gtcattccta ctgggattga attagccaag tttgagcgtc cggaaatcaa
    13681 gcaggaaaat ttgaaagaac tgcgtagtaa actagggatt caagatggtg aaaagacgtt
    13741 gcttagtctt tcgagaatct cctatgaaaa aaatattcaa gcagttttag tagcctttgc
    13801 tgatgttctg aaagaggaag acaaggttaa actggtagta gctggggatg gcccttatct
    13861 gaatgacctc aaagagcaag cccagaacct agagattcaa gactcagtca tctttacagg
    13921 gatgattgct cctagtgaga cggctcttta ctataaagcg gcggattttt tcatttcggc
    13981 atcgacaagc gaaacgcaag gtttgaccta cttggaaagc ttagccagtg gaacacctgt
    14041 cattgctcac ggaaatcctt atttgaacaa cctcatcagt gataaaatgt ttggaacctt
    14101 gtactatgga gaacatgatt tggctggtgc tattttggaa gccctgattg caacaccaga
    14161 catgaacgag cataccttat cagagaaatt gtatgagatt tcagctgaga actttgggaa
    14221 acgagtgcat gagttttatc tggatgccat tatttcaaat aacttccaga aagatttggc
    14281 taaagatgat acggtcagtc agcgtatctt taagacagtt ttgtatcttc agcaacaggt
    14341 ggttgctgta cctgtaaaag gatctagacg catgttgaag gcttcaaaaa cacagttgat
    14401 cagtatgaga gactattgga aagaccatga agaatagaaa gaggaacagc tatgaaaaaa
    14461 acaattaatg agaagcggtc gtgataaaaa gattgcgggt gtttgtgctg gggtggccca
    14521 ttatctggat atggatccga ctatcgttca agtcatttgg ggtgttctta cttgctgtta
    14581 cggagctgga attgtagctt acattatttt atggattatc gcaccagtag caactgacta
    14641 ttgaaaacta gctcaattca tgctaagata atagaaaata gaatgtaacg aaggagagaa
    14701 aaatggcatt tggagataat ggaaatcgta aaaaaactat gtttgagaaa ataaccttgt
    14761 ttatcgtgat tatcatgcta gtagcaagtt tattgggaat ttttgcaact gcaattggtg
    14821 ccctcagtaa tctataaaat agattcaaga aaatttagtg actgggattt cccagccctt
    14881 ttttaaagtg agaagaaata atgagtatgt ttttagatac agctaagatt aaggtcaagg
    14941 ctggtaatgg tggcgatggt atggttgcct ttcgtcgtga aaaatatgtc cctaatggag
    15001 gcccttgggg tggtgatggt ggtcgtggag gcaatgtggt cttcgttgta gacgaaggac
    15061 tacgtacctt gatggatttc cgctacaatc gtcatttcaa ggctgattct ggtgaaaaag
    15121 ggatgaccaa agggatgcat ggtcgtggtg ctgaggacct tagagttcga gtaccacaag
    15181 gtacgactgt tcgtgatgcg gagactggca aggttttaac agatttgatt gaacatgggc
    15241 aagaatttat cgttgcccac ggtggtcgtg gtggacgtgg aaatattcgt ttcgcgacac
    15301 caaaaaatcc tgcaccggaa atctctgaaa atggagaacc aggtcaggaa cgtgagttac
    15361 aattggaact aaaaatcttg gcagatgtcg gtttagtagg attcccatct gtagggaagt
    15421 caacactttt aagtgttatt acctcagcta agcctaaaat tggtgcctac cactttacca
    15481 ctattgtacc aaatttaggt atggttcgca cccaatcagg tgaatccttt gcagtagccg
    15541 acttgccagg tttgattgaa ggggctagtc aaggtgttgg tttgggaact cagtttctcc
    15601 gtcacatcga gcgtacacgt gttatccttc acatcattga tatgtcagct agcgaaggcc
    15661 gtgatccata tgaggattac ctagctatca ataaagagct ggagtcttac aatcttcgcc
    15721 tcatggagcg tccacagatt attgtagcta ataagatgga tatgcctgag agtcaggaaa
    15781 atcttgaaga atttaagaaa aaattgtctg aaaattatga tgaatttgaa gagttaccag
    15841 ctatcttccc aatttctgga ttgaccaagc aaggtctggc aacactttta gatgctacag
    15901 ctgaattgtt agacaagaca ccagaatttt tgctctacga cgagtccgat atggaagaag
    15961 aagcttacta tggatttgac gaagaagaaa aagcctttga aattagtcgt gatgacgatg
    16021 cgacatgggt actttctggt gaaaaactca tgaaactctt taatatgacc aactttgatc
    16081 gtgatgaatc tgtcatgaaa tttgcccgtc agcttcgtgg tatgggggtt gatgaagccc
    16141 ttcgtgcgcg tggagctaaa gatggggatt tggtccgcat tggtaaattt gagtttgaat
    16201 ttgtagacta ggagactggt atgggagata aaccgatatc tttccgagat gcggatggta
    16261 attttgtttc cgccgcagac gtttggaatg aaaagaaatt ggaagaacta tttaatcgtc
    16321 tcaatccaaa tcgtgccttg agattggcac gaactaaaaa ggaaaatcca tctcagtaaa
    16381 gaagctaaaa aatcccggct ctttgtcaac tgtagtgggt tgaaaaaaag ctaagcttga
    16441 gaaaggacaa attttgtcct ttcttttttg atgttcagag cgatgaaaat ccgttttttg
    16501 aagttttcaa agtttcgaaa accaaaggca ttgcgcttga taagtttgat gagattattg
    16561 gtcgcttcca gtttggcgtt agaatagtgt agttgaaggg tgttgacaag cttttcttta
    16621 tctttgagga aggttttaaa gacagtctga aaaataggat gaacctgctt aagattgtcc
    16681 tcaataagtc cgaaaaattt ctccggttcc ttattctgaa agtgaaacag caagagttga
    16741 tagagctgat agtggtgttt caagtcttcc gaataactca aaagcttgtt taaaatctct
    16801 ttattggtta agtgcatacg aaaagtagga cgataaaatc gcttatcact cagtttacgg
    16861 ctatcctgtt gtatgagctt ccagtagcgc ttgatagcct tgtattcatg agactttcga
    16921 tgaaactgat tcatgatttg aacacgcaca cgactcatag cacggctaag atgttgtaca
    16981 atgtgaaagc gatcaagaac aatttttgca ttcgggagtg aaacagtctg ggagactgtt
    17041 tcagcctgag cctagaaatt tgaaagcgaa gttgtttagc caagtcatag taaggactaa
    17101 acatatccat cgtaatgatt ttcacttgac aacgaacggc tctatcgtag cgaagaaagt
    17161 gatttcggat gacagcttgt gttctgcctt caagaacagt gataatatta agattatcaa
    17221 aatcttgcgc aatgaagctc atctccatct cccgattgaa acagtcactc cccggactgt
    17281 ttcaacctcc caagacataa tctcaggcag atttctaaaa ttacactcaa agtggaagtc
    17341 attgagcttg cgaatgacag ttgaagttga aatggccagc tgatgggcaa tatcagtcat
    17401 agaaattttt tcaattaact tttgagcaat tttttggttg atgatacgag ggatttggtg
    17461 atttttcttt a
//
LOCUS       DAVHIU010000033        17181 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVHIU010000033 DAVHIU010000000
VERSION     DAVHIU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.035"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            145..828
                     /gene="rpiA"
                     /locus_tag="VVN06_000942"
     CDS             145..828
                     /gene="rpiA"
                     /locus_tag="VVN06_000942"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU5562515.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            842..2053
                     /locus_tag="VVN06_000943"
     CDS             842..2053
                     /locus_tag="VVN06_000943"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU5562516.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            2055..2597
                     /locus_tag="VVN06_000944"
     CDS             2055..2597
                     /locus_tag="VVN06_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU5562517.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLGTFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            2613..3422
                     /locus_tag="VVN06_000945"
     CDS             2613..3422
                     /locus_tag="VVN06_000945"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5562518.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3898..4293
                     /locus_tag="VVN06_000946"
     CDS             3898..4293
                     /locus_tag="VVN06_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU5562519.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4303..4877
                     /locus_tag="VVN06_000947"
                     /pseudo
     CDS             4303..4877
                     /locus_tag="VVN06_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            4902..5414
                     /locus_tag="VVN06_000948"
     CDS             4902..5414
                     /locus_tag="VVN06_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU5562520.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            6068..6778
                     /gene="deoD"
                     /locus_tag="VVN06_000949"
     CDS             6068..6778
                     /gene="deoD"
                     /locus_tag="VVN06_000949"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5562521.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(6801..7325)
                     /locus_tag="VVN06_000950"
     CDS             complement(6801..7325)
                     /locus_tag="VVN06_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU5562522.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(7378..7614)
                     /locus_tag="VVN06_000951"
     CDS             complement(7378..7614)
                     /locus_tag="VVN06_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5562523.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(7682..8602)
                     /locus_tag="VVN06_000952"
     CDS             complement(7682..8602)
                     /locus_tag="VVN06_000952"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU5562524.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            8792..9195
                     /locus_tag="VVN06_000953"
                     /pseudo
     CDS             8792..9195
                     /locus_tag="VVN06_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9411..9992
                     /locus_tag="VVN06_000954"
     CDS             9411..9992
                     /locus_tag="VVN06_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5562525.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            9989..11266
                     /locus_tag="VVN06_000955"
     CDS             9989..11266
                     /locus_tag="VVN06_000955"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU5562526.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            11284..11946
                     /gene="deoC"
                     /locus_tag="VVN06_000956"
     CDS             11284..11946
                     /gene="deoC"
                     /locus_tag="VVN06_000956"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5562527.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            11933..12322
                     /locus_tag="VVN06_000957"
     CDS             11933..12322
                     /locus_tag="VVN06_000957"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5562528.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPILPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            12413..13465
                     /locus_tag="VVN06_000958"
     CDS             12413..13465
                     /locus_tag="VVN06_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU5562529.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            13609..15144
                     /locus_tag="VVN06_000959"
     CDS             13609..15144
                     /locus_tag="VVN06_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562530.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            15137..16195
                     /locus_tag="VVN06_000960"
     CDS             15137..16195
                     /locus_tag="VVN06_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562531.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            16198..17154
                     /locus_tag="VVN06_000961"
     CDS             16198..17154
                     /locus_tag="VVN06_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562532.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         5038 a         3053 c         3941 g         5149 t
ORIGIN      
        1 gataaactca tttttatttg tttggtaaaa ataaaatgca tatttttaaa gaaagattga
       61 tgaaaaatgt agatttgatg tttttacttg aataaaaagc ggtttcatag taaaataaga
      121 actgagaatg atggaggtaa atcggtggaa aatctgaaga aaatggcagg tatcaaggct
      181 gctgagttcg tgagcgatgg aatggtcgtt ggacttggaa caggctcgac tgcctattat
      241 tttgtcgaag aaatcggtcg tcgtatcaag gaagaaggat tgcagattac agctgtaaca
      301 acttctagtg tgaccactaa acaggcagaa ggccttaata tcccgctcaa gtctattgac
      361 caagtagact ttgtcgatgt gacggttgat ggcgcggatg aagtggatag tcagtttaat
      421 ggaatcaaag gcggtggtgg tgcccttctc atggaaaagg tggtcgcaac accatcaaaa
      481 gaatacattt gggtggtgga tgaaagcaag ctggtcgaaa aactaggtgc ttttaaattg
      541 ccagtagaag tggttcagta tggtgcagag caggtctttc gtcattttga acgagctggc
      601 tacaaaccaa gtttccgtga aaaagacggc caacgttttg tgacggatat gcagaatttt
      661 atcattgacc tcgccttgga tgtcattgaa aatccaattg cttttggaca agaattggat
      721 catgtcgttg gtgttgtgga gcatggttta ttcaaccaaa tggtggataa ggtaatcgtt
      781 gctggacggg atggagttca gatttcaact tcaaaaaaag gaaaatagaa gggggcataa
      841 gatgtctaaa tttaatcgta ttcatttggt ggtactggat tctgtaggaa tcggtgcagc
      901 accagatgct aataactttg tcaatgcagg ggttccagat ggagcttctg acacactggg
      961 acatatttca aaaacagttg gtttgaatgt cccaaacatg gctaaaatag gtcttggaaa
     1021 tattcctcgt gaaactcctc ttaagactgt agcagctgaa agcaatccaa ctggatatgc
     1081 aacaaaatta gaggaagtat ctcttggtaa ggatactatg actggacact gggaaatcat
     1141 gggactcaac attactgagc ctttcgatac tttctggaac ggattcccag aagaaatcct
     1201 gacaaaaatc gaagaattct caggacgcaa ggttattcgt gaagccaaca aaccttattc
     1261 aggaacggct gttatcgatg attttggacc acgtcagatg gaaactggag agttgattat
     1321 ctatacttca gctgaccctg ttttgcagat tgctgcccac gaagacatta ttcctttgga
     1381 tgaattgtac cgtatctgtg aatacgctcg ttcgattacc cttgagcgtc ctgcccttct
     1441 tggtcgcatc attgctcgcc cttatgtagg tgaaccaggt aacttcactc gtacggcaaa
     1501 ccgtcgtgac ttggctgtat ctccattttc cccaactgtt ttggataaat tgaatgaggc
     1561 tggtatcgat acttatgctg tgggtaaaat caacgatatc tttaacggtg ctggtatcaa
     1621 ccatgacatg ggtcacaaca agtcaaatag tcatggaatt gatacactat tgaagactat
     1681 gggacttgct gagtttgaaa aaggattctc attcacaaac ctagttgact ttgatgccct
     1741 ttacggccat cgtcgtaatg ctcacggtta ccgtgattgc ttgcatgagt ttgatgaacg
     1801 cttacctgaa attatcgcag ctatgagaga gaatgacctt ctcttgatta ctgcggacca
     1861 tggaaatgac ccaacgtatg caggaacgga tcacactcgg gaatatattc cattgttggc
     1921 atatagccct gcctttaaag gaaatggtct cattccagta ggacattttg cagatatttc
     1981 agcgactgtt gccgataact ttggtgtgga aactgctatg attggggaaa gtttcttaga
     2041 taaattggta taatatgacg cgctatgctt tgctggtgag aggtatcaat gttggtggta
     2101 agaataaggt cgtcatggcg gagcttcgtc aagaattgac aaacttggga ctggaaaagg
     2161 ttgagagcta catcaacagt ggcaatattt tctttacttc gatagattcc aaagcccaat
     2221 tggttgaaaa gctagggact ttctttgcag tccattatcc atttattcag agcttttctt
     2281 tactgagtct agaggacttt gaggcggaac ttgaaaatct accagcttgg tggagcagag
     2341 acttggcacg aaaagatttt ctcttttaca ctgagggttt ggatgtggac caagtcatcg
     2401 cgacagttga aagtttagag ctgaaagatg aagtgcttta ttttggaaaa cttgggattt
     2461 tctgggggaa attttctgaa gaatcctatt ctaagactgc ctatcagaag tacttgctga
     2521 aggtgccttt ctaccgccac attactattc gtaatgctaa aacctttgac aaaattggtc
     2581 aaatgctaaa aaaataataa aggagacaca caatgacatt tttaaacaaa atccatgaaa
     2641 ctgctacttt cctgaaagaa aagggaattg cagcccctga gttcggtcta atccttggat
     2701 caggacttgg agaattggca gaagaaatcg aaaatccagt tgtagtagac tatgctgaga
     2761 ttccaaactg gggccgttca acagtagtcg gtcatgctgg taaattggta tatggtgaac
     2821 tggcaggtcg caaggtcttg gctcttcaag gtcgtttcca tttctatgaa gggaatcctc
     2881 tggaagtggt gactttccca gttcgtgtga tgaaagttct tggatgtgaa ggtgttattg
     2941 taaccaatgc agctggcggt atcagatttg gtcctggtac cttgatggct atctcagacc
     3001 atatcaacat gacggggcaa aatccattga tgggtgaaaa cttggatgac tttggcccac
     3061 gtttcccaga tatgtctagg gcctacacac cagaataccg tgccactgcc catgaagtgg
     3121 ctaaaaaact taatatcaag cttgatgaag gtgtctatat cggagttact ggtccgactt
     3181 atgaaacacc agcagaaatt cgttcctata agacactggg agcagatgca gttggtatgt
     3241 ctacggttcc tgaagttatc gtggcagccc actctggctt gaaagttctg ggaatttcat
     3301 gtatcactaa ctttgcggct ggtttccaag aagaactcaa tcacgaagaa gttgtagaag
     3361 tgactgaacg tgttaaaggt gatttcaaag gcttgcttaa agcgattctt gctgaattgt
     3421 aagaaaaaag atttaaaagg ggtagtgtct ctgtcttttc aggattgact gcctatccgt
     3481 attaaagaag aaacagagga atactatgag cttcttcctg ctcttataac tgaaagaagc
     3541 ggaagaatag gtgtgtctga tctgatagcc agcattgtga aagacaagat tctaggatac
     3601 tagcattagc ttcctagcca agcagactag tatgataagg agagatgaga atgaattgac
     3661 tttctgaatt tctcagtctt atcatatata gcacaatgag atttcgcttg agtctgcttg
     3721 taaataaacg aaaagaaaga taagaaataa tgaaaattgg tcaacgaatt atgcgctttg
     3781 gcataaaaaa ttaagtatcg gagttgtatc tgttgtagtc ggctttgatt tctagctcca
     3841 gctggaattt cagccaatga agtaaagcaa gatgtaacat ctgaagtggt aataggtgtg
     3901 ctagattcta aggaggaatt gaaagagtca gaaaatgatg ctccaaaact agaaactcct
     3961 cttagagagg agccaagact agctcctcaa acgcttccgg aagcaagtga agttcttgaa
     4021 aacaaaaggg aagagtcaaa agtagagata acagagccag ctcaagcgga tgatatccgc
     4081 aaggttgttg gggaattagc caaggatata agtattacta agttgtatat gacaggtcat
     4141 tctcttggag gctacctagc tcagattgca gcggttgaag attaccaaaa atatcctgat
     4201 ttttataacc atgtattgag gaaagtgaca actttcagtg ctcctaaagt gattacttcc
     4261 agaactgttt gggatgctaa gaatggtttc tgagatgttg gtttggaaag tcgtaaatta
     4321 gctgttagtg gaaaaattaa gcattatgtg gttgataatg acaatgttgt gactcccttg
     4381 attcataata atcgtgatat tgttacattt acaggtaatt cacgctttaa acaccgttct
     4441 cgtggctatt ttgaaagtcc aatgaatgat attcctaact ttaatattgg taaacaagct
     4501 accttggata aacatggtta tcgtgatccg aaattggata aagcgcgatt ctttaagaaa
     4561 caggctctac ctcaatcttc tagtcaacca agcgctgaac caatggaaaa tattgcctta
     4621 ggaaaacagg ttactcaaag ttcgacagct ttcggaggaa tgctagaaga gctgtggatg
     4681 gcaaagtcga tggtaactat ggtcacaatt ctgtcactca tacaaacttc caatctaagc
     4741 cttggtggca agtagatttg gctaaagaag aaaccattcg ccaaatcaat atttacaacc
     4801 gaacagacac tgcccaggat agattggcaa actttgatgt cattctttta gacggttctg
     4861 gtaaagaaat tgagtgaaaa cgtataatat ctcctaaaga tgtgtcagca caaattacga
     4921 ttaaccataa aaaagcgcgc tatgttcgga ttgagctaga aggctataat gccctcagtc
     4981 ttgcagaagt tgaagttttc cgctttatag ctacgaatgg tgaaacggcg atacaagttt
     5041 ctaagtcagt tcaaccaatc agtcagactc ctgtgaaaga taaaacattg acaattcaac
     5101 acagtggagc ttacattgcc cgctactcca taacttggga agaagttaca gtagataaag
     5161 atggaaacca agttgttcgt agtcattctt gggaaggaaa cggtcgcaac cagactgcag
     5221 gttttgtcct caacctccca atcaaagaaa atatgagaaa tctgcgagtt aagattgaga
     5281 aaaagacggg cctactatgg aatagatggc aaacaatcta tgaaaacaga ccaattttag
     5341 ctcaacccca ccgtaaaatt acccattggg gtacgacatt gaattccaag gtgagtgacg
     5401 atgatgtctt gtaatctgat ggtacaatga cagttagttt gtctagttta taagaaagta
     5461 ctacctgagc ttgaatagaa ctcaggtagc tctctgtgaa agaacaaaat taatactcaa
     5521 tgaaaatcaa agagcaaact aagaaactag ccgcaggttg ctcaaagcac tgctttgagg
     5581 ttgtagataa gactgacgaa gtcagtcaca tatataatcc aaggcgacgt tgacgtggtt
     5641 tgaagagatt ttcgaagagt ataaacagaa aggtagagcg cgtgttctaa tttgaacacg
     5701 agtagaaaac ttttctaaaa acaaaaacga aaggatgggt aaactgtatt cgctgaactg
     5761 aatacgggcg actctcctct aaatcaaaat taagaaagga attgacccca ccctaaaagt
     5821 agtgggaaaa agatagttgg tctagcgagc atcgctcact gcgcccaact cctattttcc
     5881 cttcgctttt tgatgggttt ggtatctttc tcaatataaa atataaaata aagaaaggta
     5941 gagcgtgtgt tttgatttga acacgagcgg aaaactcgga aaatagataa tctgactgaa
     6001 aaatcaggat ttctcgtcag gttcctaatt ttcagtcgtt ttcttctcgc tctttgtatc
     6061 ataaattatg tctatccata ttgctgctca gcagggtgaa attgctgata aaattcttct
     6121 tcctggggat cctcttcgtg ctaagtttat tgcggagaat ttccttggtg atgctgtttg
     6181 ttttaacgaa gtgcgtaaca tgtttggtta cactggtact tacaagggtc accgtgtatc
     6241 tgtcatggga actgggatgg gaatgccatc tatttcgatt tatgcgcgtg agttaatcgt
     6301 agactacggt gtgaagaaat tgattcgtgt gggaactgca ggttctttga atgaagaggt
     6361 tcatgttcgt gaattagttt tggcgcaggc ggctgcaacc aactcaaaca tcgttcgtaa
     6421 tgactggcca cagtacgatt ttccacaaat tgctagcttt gatttgcttg ataaagccta
     6481 ccatatcgcc aaagaacttg gtatgactac tcacgttggg aacgttttgt catctgatgt
     6541 cttttactca aattactttg aaaagaatat cgagcttggt aaatggggag tcaaggctgt
     6601 ggaaatggaa gcagcagctc tttactatct tgctgcccaa taccatgttg atgcgctagc
     6661 tatcatgacc atctctgata gcttggtcaa tccagacgaa gacacaactg cagaagaacg
     6721 tcaaaatacc ttcactgata tgatgaaggt tggtttggaa accttgattg cagaataatt
     6781 atagccaaaa aggaagccct ttatgaattg taggacttcc ttttcttatc cagaaattga
     6841 tctagctctc tctgatttcg aagaatagtg actttatgtg aatattcttg gcaaagtttt
     6901 tggtaatttt ctttttgagt tttgctacgc ccatcccaaa gaatccatct gataaactcc
     6961 cagtcaaagc gttcagggca atctgccgcc atactttctc tgacttttcc acggtattta
     7021 agataacgct taaaggctct aaagagacag gtcaatggca aaaaattgag aaagatgatt
     7081 tggtcagctt cttgcattcg ttcttggtag tagcaccaag aataattacc atcgatgacc
     7141 caagctttat gcttggtgag aaagtttttt atctcggtta acatccattc gcagtcactg
     7201 tcttgccaac caggttgaaa ttggagtgtg tccatgtgca gttttggaat ggagtagtag
     7261 ttagataact tttctgctag agttgactta ccagaaccag aatatccgat aattgtgatt
     7321 ttcattttct accttttcct atttggagac aaaaaaacag cctctatgga ctgtttctta
     7381 tttagcaagt ttagttgaaa gacgagcttt atcgcggctt gctttgtttt tgtgaatcaa
     7441 acctttagtt tctgctttat cgatagctga gctagcagca cggaaaagtt cttcagatgg
     7501 gtttgcttcg aaagctttta tagcagtacg catagctgat ttttgagctg agttcttttc
     7561 gttttgttta acgttcaatt cagcgcgttt gatagctgat ttaatgtttg ccaatgttct
     7621 tacctccata tttactaact ataccattat atatgaaaac ttacgttttg acaagggaaa
     7681 attacttttt taagtaaatt tcatcgattt catggttctt gcttttatga agaatcactt
     7741 ctgcacgatt tctggttggt tcaatataat tttgcagatt tgtgagattg atactgatcc
     7801 agacctgatg ggcaaaggac tccacttccc caatcggcat ctgagtaaaa cgataatagt
     7861 agctatcagg gtcgttttgg gctaggctca gcattttcaa gaaacggtcc agataccaac
     7921 tttcaatatc atccactcca gcatctacat agatggaaaa gtcaaagaag tcagtgatat
     7981 agagacgatc gttttgtgga ttttgaaaga cattaattcc ctcaacaatt acaaaatcag
     8041 cagctttgac actttgtttc ttttcgggta cgatgtcgta aacttcatga gaatagacag
     8101 gaatatctac atcttgtcca tttttgatgc ggtccaagaa gttgagaaga gcttccatat
     8161 catagctttc aggaaatcct ttacgattta aaatcccctg ctcaatcaag gtttgattgg
     8221 gatatagaaa accatcagtt gtaaccaact caaccgtagc atctgtaaac gtacgggaca
     8281 gtaggatttg aagtaggcga ctggttgtgg attttccaac ggcaacactc ccagaaaccc
     8341 caataataaa aggttgagat ttactttcac gttggaggaa aattcctttt gaaaaggcta
     8401 aatcttcctt agttcgcttg taaatctgaa tcagatgagc caaggggaga tagatatctg
     8461 taacgtcttg gagactgatt tggtcattaa aactcttgat agattccaat tcttcttctg
     8521 tcaaaggagg tgttgtcttt cgatgtaaag attgccaagt ctggcggctg attttttcaa
     8581 aatgtaaaaa ttcgttggtc atttgttttc ccctagatat tttgtctatc ataccataaa
     8641 aagctaaaaa aataaagcgg tttcttgaat gtggtaagtc aatggtatat agtataagtg
     8701 atcaatttat tgatgaaata ggagatagat tctactgatt tataagctta taaagggggc
     8761 tttttagttc acaacttata ggaggtgtac tatgaaaatc ttaaaacgtt acatattgga
     8821 actctgtttt attttaagtt ttgctttacc ttttataaaa ggaaccaatg cagataatgg
     8881 tagatgcttt gtggaaacct attacggttt tacttttttg atggaacatg ctattgtaac
     8941 agctgtcttt atctgttcgt tcttaattgc tttcttacta aaaaacgatg gacgaaatgg
     9001 attgctgcgg gtagttattg ctttttagtc ctacggattg cgacagaggg ttatttcttc
     9061 cacatgtcac tagaagattt gatacgactt tggacaagtt tggaaatcct gacacaaacg
     9121 tatcagttgg gcttttatct aaacattttg ttaggaattt tgttgataat aaagtatttt
     9181 aaggttaagc aatagaaata ataatctgcc tgattataga tgataaactt tttgttttta
     9241 tctggactgt gatatttata gttctaaatg gtttattatt tataagtgtt gaaagagatt
     9301 gaagaaaatg gcttcgtgac ttgctttctt cggtcttttt cgttttatta tcaatttgta
     9361 aacgatttac aatttgagtc aaattatggt aaaatagtgt catgagtaaa atgtactatg
     9421 cagaaaatcc tgacgctgct cacgacattc atgagttgag agtggacttg ttgggagaaa
     9481 aaatgacttt tttgacagat gcgggtgttt ttagcaagaa aatggttgac tttggaagtc
     9541 aactcttgct caagtgtctg gatgtcaacc aaggagagac agtcctagat gtaggctgtg
     9601 gctatggtcc attaggtttg tccttggtca aggtttacgg agttcaggcg accatggttg
     9661 atattaacac tcgtgccctg gatttagctc agagaaatgc tgaaaaaaat aatgcaaaag
     9721 cgacgatatt tcaatctaat atttatgcaa gagttcaagg gcgatttgac catgtcattt
     9781 ccaatcctcc tatccgtgcg ggcaagcaag tcgttcatga aatcatcgag aaaagtaaag
     9841 atttcttgga aattggtgga gatttgacca tcgtcatcca gaaaaaacaa ggagctccaa
     9901 gtgccaagtc taagatggaa gatgtgtttg gcaattgtga aatccttaaa aaagacaagg
     9961 gatactatat ccttagaagt gtgaaagaat gagagcagtt gatttaatcc aaaaaaaacg
    10021 agacggtcaa gaactgactt caagtgaaat tgaatggctg atagaaggct atgtatcagg
    10081 aactgttcct gattatcaga tgtctgcctt tgctatggct gtttatttta aaggaatgac
    10141 gactcgagaa atctctgatt taacgatgaa tatggttaag actgggcaag agtttgacct
    10201 gtcagccatt gatggtgtga aagttgacaa gcattctact ggtggtgtcg gcgataaggt
    10261 aaccttgatt ttagctcctc ttgttgctag cttcggtgtg cctgtagcaa aaatgagtgg
    10321 tcgtggtctt ggccatacag gcggaacaat tgataaattg gagtccatta agggctatca
    10381 agtagaacgt agtcaagagg attttattcg tcaggttcag gatattggag tatctgtcat
    10441 tgggcaatca gaccagctgg tcaaagcaga taagcttctc tatgcccttc gtgatgtgac
    10501 cgcaactgtc gacacaattc ctttgattgc gagttcggtg atgagcaaga aaattgcggc
    10561 aggggcggat gctattttgc tagacgtgac tgtcggtgag ggtgccttca tgaagacggt
    10621 tgatgaggcg cgtgagttgg ctcaaactat ggtagatctt ggtaaggttg ttggacggaa
    10681 gacagtagca gtgattacgg atatgagcca gcctttggga cgagcgattg gaaatcgtct
    10741 ggaaatcctt gaagcattgg agattttgca aggtcaagga cgtcaggata ttacccactt
    10801 tatttgtgaa ttggctcaaa ttatgcttgg tttggcaaat gttaacaaaa ctgttgaaga
    10861 agttcgccaa catcttgaaa atggtcaagc actggctaag tttgaggaaa tggtgcaagc
    10921 ccaaggtgga gacttggaag acctctatcg tcctgtaaat gtagctcatg tggtggaaat
    10981 ccctgctcag gaaacgggtg tcatttcagc tcttccagct atggactttg gtctttatgc
    11041 catgaggctg ggtgctggtc gtgcagttaa gtctgatgtc ttggactatg aaacaggaat
    11101 tgtttttgaa aagaagattg gagactccgt tcaaaagggt gaaattgttg caaaagtata
    11161 cacaaatgaa aaaattccac ctcaactagt tacagatttc caaaaatgtg ttaaaataag
    11221 tgatgaagtg aaaaaaatcc gtgaaattgt agaaatcatc tcctaaatgg agggaaaacc
    11281 gaaatgaaat taaataaata tatagatcat acgcttttaa aacaagatgc aaagaaaaaa
    11341 caaattgata gtttgttgtc tgaggctaga gagtatgact ttgccagtgt ttgcgttaat
    11401 ccgacctggg ttgaacatgc taaaaaagga cttgaaggca cagatgttaa ggtttgcaca
    11461 gtagtaggtt tccctttggg agcaacaact tcagccgtga aagcatttga gacaaaagaa
    11521 gctatccaaa atggtgcaga tgagattgat atggtgatca atgttggagc tctcaaatca
    11581 ggtaatttag ccttggttga gtcagatatt cgcgcagtag tggaagcaag tggtgataag
    11641 ttagtgaaag tcattattga agcttgcctt ctgacagacc aagaaaaaat tgttgtttgc
    11701 caattggccc aaaaagctgg ggctgacttt gttaaaacat ctactggctt ttcaactggt
    11761 ggtgctacga tagcagatgt tagattaatg cgtgaaacag ttggatctga tatgggagtc
    11821 aaggctgctg gtggagctcg ttcttatgca gatgctcttg cctttgtcga agcaggtgcg
    11881 acccgtatcg gaacgtcagc tggggtagct attttaaaag gagaattggc agatggcgac
    11941 tactgagttg attgaactgg caattgaaac cagcaaacat gcctatgtcc cctattctca
    12001 ctttcctatc ggagcagttt tagtagccaa agacggaagt gtttacacgg gagtgaatat
    12061 cgaaaatgct agctatcctt tgaccaattg cggtgagaga acagctgttt ttaaggctat
    12121 atctgaaggc caaagagaat tttcagaatt gattgtctat ggtcaaactg aaaaaccgat
    12181 tttaccatgt ggtgcttgtc gccaagtaat ggtcgaattt tttgaacaag atttaaaagt
    12241 gaccttagtc gcaaaagata aatcgacggt cgagatgacg gtcggggagt tacttccata
    12301 ctcttttaca gacttaaatt agtctgagtc gctctttgag tggcaagggt ctttgtgacc
    12361 aatcaatcca tacttgcaac atcgttgcac atcttattta ggaggttcag taatgaacaa
    12421 gaaacaatgg ctaggtcttg gcctagttgc agtggcagca gttggacttg ctgcatgtgg
    12481 taaccgctct tctcgtaacg cagcttcatc ttctgatgtg aagacaaaag cagcaatcgt
    12541 cactgatact ggtggtgttg atgacaaatc attcaaccaa tcagcttggg aaggtttgca
    12601 agactggggt aaagaacaca atctttcaaa agataacggt ttcacttact tccaatcaac
    12661 aagtgaagct gactacgcta acaacttgca acaagcggct ggaagttaca acctaatctt
    12721 tggtgttggt tttgcccttc acaatgcagt tgaagaagca gctaaagaac acactgactt
    12781 gaactatgtc ttgattgatg atgtgattaa agaccaaaaa aatgttgcta gcgtaacttt
    12841 cgctgataat gagtcaggtt accttgcagg tgtggctgca gcaaaaacaa ctaagacaaa
    12901 acaagttggt tttgtaggtg gtatcgaatc tgaagttatc tctcgttttg aagcaggatt
    12961 caaggctggt gttgcgtcag tagacccatc tatcaaagtc caagttgact acgctggttc
    13021 atttggtgat gcggctaaag gtaaaacaat tgcagccgca caatacgcag ccggtgcaga
    13081 tattgtttac caagtagctg gtggtacagg tgcaggtgtc tttgcagagg caaaatctct
    13141 caacgaaagc cgtcctgaaa atgaaaaagt ttgggttatc ggtgttgatc gtgaccaaga
    13201 agcagaaggt aaatacactt ctaaagatgg caaagaatca aactttgttc ttgtatctac
    13261 tttgaaacaa gttggtacaa ctgtaaaaga tatttctaac aaggcagaaa aaggagaatt
    13321 ccctggcggt caagtgatcg tttactcatt gaaggataaa ggggttgact tggcagtaac
    13381 aaacctttca gaagaaggta aaaaagctgt cgaagatgca aaagctaaaa tccttgatgg
    13441 aagcgtaaaa gttcctgaaa aataatggat ggaagtcatt tcttaggaag cggccaaggg
    13501 ccgctttttt aagagttgag acaaagtcat tttgtctcag taaagcttgc tactagacaa
    13561 actagcaagt tttattgaaa taaaataaga ctctgaaagg aagagcacat ggcacacgaa
    13621 aatgtcattg agatgcgtga tattaccaag gtgtttggtg gatttgttgc caacgacaaa
    13681 atcaacttgc acctacgaaa aggtgaaatt catgcacttt taggagaaaa tggggctggt
    13741 aagtccacgc taatgaacat gttagcaggc cttcttgaac caactagtgg tgaaatcgcg
    13801 gtcaacggtc aagttgtcaa tctcgactcc ccatctaaag cagctagctt gggaatcggg
    13861 atggttcacc agcactttat gttggttgaa gccttcacag tggctgaaaa catcatttta
    13921 ggtagtgaat tgactaaaaa tggtgtgcta gatatcgctg gagctagcaa agaaatcaag
    13981 gctctttctg aacgttatgg cttagctgtt gacccttctg ccaaggtagc agatatctca
    14041 gttggagccc aacaacgtgt agaaatttta aaaacacttt atcggggggc tgatatcctt
    14101 atctttgacg aaccaacggc tgttttgact ccatcagaaa ttgatgagtt gatggctatt
    14161 atgaaaaatc ttgtcaaaga agggaaatca attatcttga ttacccacaa attggatgaa
    14221 attcgagcag tttctgaccg tgttacagtt atccgtcgtg ggaaatcaat tgaaaccgtt
    14281 gaaattgcag gggctaccaa tgctgatttg gcggaaatga tggtaggacg ttctgtttcc
    14341 tttaaaacag agaagcaagc ctctaaacca aaagaagtgg ttttgtctat caaagatttg
    14401 gtggtcaatg aaaaccgtgg tgttccagct gttaaaaatc tgtccttgga tgttcgtgct
    14461 ggagagattg ttggtattgc ggggattgat ggaaatggtc agtctgaact gattcaagcc
    14521 attacaggtc ttcgcaaggt tgaatctggt agcattgagc taaaaggaga ttcaattgta
    14581 ggcttgcacc cacgtcagat tacagaacta agtgttgggc acgttccaga agaccgtcac
    14641 cgtgatggct tgattttgga aatgatgata tctgaaaata ttgcccttca aacctactat
    14701 aaagaaccac atagtaaaaa tggaattttg aattattcaa atattacttc ttatgctaaa
    14761 aagctgatgg aagagtttga tgttcgcgct gccagtgaat tagttcctgc agctgcactc
    14821 tcaggaggaa atcaacaaaa agcaattatt gctcgtgaaa ttgatcgaga tcctgatctc
    14881 cttatcgtta gccagccaac tcgtgggttg gatgtcggtg ccattgagta tatccacaaa
    14941 cgcttgattg aagagcgtga taatggcaag gctgtccttg ttgtcagctt tgaattggat
    15001 gagattttaa acgtctcaga ccgtattgcc gttatccacg atggtaagat tcaaggtatt
    15061 gtatcaccag aaacaaccaa taaacaagaa cttggtgtct tgatggctgg tggaaacttg
    15121 ggaaaggaga agagtgatgt ctaaaaaatt acaacaaatt tcggttccct tgatttctgt
    15181 attcctagga attttactcg gagccattgt catgtggatc ttcggttatg atgctatttg
    15241 gggctacgaa gaattgttct atacagcctt tggcagtctg cgtgggattg gagaaatctt
    15301 ccgtgctatg ggtcctctgg tcttgattgg tcttggtttt gccgttgcca gtcgagctgg
    15361 tttctttaac gtcggacttc ctggtcaggc tttggcaggt tggattctca gtggttggtt
    15421 tgccctgtcg catccagata tgccccgtcc cttgatgatt ctagcaacca tcgtgattgc
    15481 cttgattgct ggtgggattg tcggagcgat tccaggtatt cttagggcct atctagggac
    15541 gtcagaggtt attgtaacca tcatgatgaa ctacattgtc ttgtatgtag ggaatgcctt
    15601 tatccatgct ttccctaaag acttcatgca aagtacagat tcgaccattc gtgttggggc
    15661 taatgcaacc tatcagacac cttggttggc tgagttgact ggtaactcac ggatgaatat
    15721 tggtattttc tttgccatca ttgccgttgc agttatttgg ttcatgctca agaaaacaac
    15781 tcttggtttt gaaatccgtg cagttggtct taatccacat gcttcagaat atgctggtat
    15841 ttctgccaag cggactatta tcctatctat gattatttca ggtgccttgg caggtcttgg
    15901 tggagctgtt gaaggtttgg gaaccttcca gaacgtctat gttcaaggtt cgtcattagc
    15961 tatcggattt aacggaatgg cggttagttt gcttgcggcc aactcaccaa ttggtatact
    16021 ttttgcagcc ttcctatttg gcgttctcca agttggggct cctggtatga atgcggcgca
    16081 ggtaccatct gagcttgtca gcattgtaac agcgtctatt atcttctttg tcagtgttca
    16141 ttaccttatc gaacgctttg tcaaaccgaa aaaacaagtt aaaggaggta agtaaagatg
    16201 tctattataa ccttgctccc cctcttggtg tcttctatgc tgatttactc agcacctctc
    16261 atctttacaa gtatcggtgg tgttttctct gaacgtggtg gtgtggtaaa cgttggcctt
    16321 gaaggaatta tggttatggg tgccttttct ggagttgtct ttaaccttga atttgcagaa
    16381 caatttggag cagcaactcc atggctatcc ttgcttgtag caggattggt tggtagtgtt
    16441 ttctctatca tccacgcagc agcgacggtt catttccgtg cagaccatgt tgtcagcggt
    16501 acggtattga acttgatggc gcctgccttg gctgttttct tagttaaagt tctttataac
    16561 aaaggacaaa ccgacaacct aagtcaaact tttggacgct ttgatttccc agtcttggca
    16621 aatatcccag tgattggtga tatcttcttc aagtcaacta gtctacttgg ttatctggcg
    16681 attgccttct cattccttgc ttggtttatt ctctttaaga ctcaatttgg tcttcgtctc
    16741 cgctctgtcg gtgaacatcc tcaagcagcg gataccttgg gaatcaacgt ctacaagatg
    16801 agatatttag ggattattat ttcaggtttt ctaggtggaa ttggcggagc gatttatgcg
    16861 caatcaatct cagttaactt ctcagtgaca actattgttg gacctggatt tatcgccctt
    16921 gctgcgatga tctttgggaa atggaatcca atcggtgcta tgctttctag tctcttcttt
    16981 ggactttcac aaagtttggc tgttatcggt tctcaattgc cgttcctaca aggagtgcca
    17041 gcggtttatc ttcaaattgc accttatgtt ttgacaattc ttgtcttagc agccttcttt
    17101 ggaaaagcag tcgcaccaaa agcagatggt atcaactaca tcaaatcaaa ataagcatac
    17161 aaaaaaacgt cagtccaatg a
//
LOCUS       DAVHIU010000034        16763 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVHIU010000034 DAVHIU010000000
VERSION     DAVHIU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.036"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..552
                     /locus_tag="VVN06_000962"
                     /pseudo
     CDS             1..552
                     /locus_tag="VVN06_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(545..712)
                     /locus_tag="VVN06_000963"
     CDS             complement(545..712)
                     /locus_tag="VVN06_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU5562533.1"
                     /translation="MKKRILSAGLTFTSALLLAACGQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(877..2484)
                     /locus_tag="VVN06_000964"
     CDS             complement(877..2484)
                     /locus_tag="VVN06_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU5562534.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2637..4121
                     /locus_tag="VVN06_000965"
     CDS             2637..4121
                     /locus_tag="VVN06_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU5562535.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINVIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4216..4698
                     /locus_tag="VVN06_000966"
     CDS             4216..4698
                     /locus_tag="VVN06_000966"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU5562536.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4993..7098)
                     /locus_tag="VVN06_000967"
     CDS             complement(4993..7098)
                     /locus_tag="VVN06_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5562537.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
     gene            complement(7662..8642)
                     /locus_tag="VVN06_000968"
     CDS             complement(7662..8642)
                     /locus_tag="VVN06_000968"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5562538.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIAAVVVSFLVALFSKQLTNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIIFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVIFAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTFAILYLM"
     gene            complement(8644..10896)
                     /locus_tag="VVN06_000969"
     CDS             complement(8644..10896)
                     /locus_tag="VVN06_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU5562539.1"
                     /translation="MKWTKKVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPKLKQVSFGSKGNGITY
                     ANMMAIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGKDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSTLQSFMETQ
                     MNAFQEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNEGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGKPVSEDTA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSAVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKIKLTLGD"
     gene            complement(10900..11217)
                     /gene="ftsL"
                     /locus_tag="VVN06_000970"
     CDS             complement(10900..11217)
                     /gene="ftsL"
                     /locus_tag="VVN06_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU5562540.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(11230..12180)
                     /gene="rsmH"
                     /locus_tag="VVN06_000971"
     CDS             complement(11230..12180)
                     /gene="rsmH"
                     /locus_tag="VVN06_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5562541.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQACLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            12346..12540
                     /locus_tag="VVN06_000972"
     CDS             12346..12540
                     /locus_tag="VVN06_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5562542.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEDNF"
     gene            12556..13098
                     /locus_tag="VVN06_000973"
     CDS             12556..13098
                     /locus_tag="VVN06_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU5562543.1"
                     /translation="MKKETFTEKLIKRIYGISGPLDEHKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYKMKKTGITAIDPDMLSEKE
                     SKQLYYPGLKAGLFFGLWMFFITPLLSILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKAKEDQDED"
     gene            13109..13435
                     /locus_tag="VVN06_000974"
     CDS             13109..13435
                     /locus_tag="VVN06_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562544.1"
                     /translation="MKSLLTLLTYHLLFSSLLIFIIISGNLPIDETFLVLVFILALNK
                     GLTYLKIDSPKTRTLNLALYFMILSFPQLMLISSDWKYYLLLTIASLSSITYLYYLYQ
                     FVKESQ"
     gene            13462..14040
                     /locus_tag="VVN06_000975"
     CDS             13462..14040
                     /locus_tag="VVN06_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU5562545.1"
                     /translation="MNKEDLTTRLLRNLFHIQGPFDECRQEIIYKACARSMVQIVYSS
                     FFLFLFYLLFGRFIEAVRYAMPYVYSGLIFFITLKTQRAVKELHLEKDDKSEIILKTY
                     SKAQIKFRSWVVFIGIQIGLFTLLIFHKVFVQQMSFSDFGKLLMQFDKSGPFLMYGLI
                     IGSIFGTLTYGFLSLQEEKTPKNTKQKEKSKQ"
     gene            complement(14311..15312)
                     /locus_tag="VVN06_000976"
     CDS             complement(14311..15312)
                     /locus_tag="VVN06_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5562546.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSTQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSRLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHARLT
                     IEDKHTNLEQIKEFLQKEINPDEKTLVFVPNCWALPPVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPAFEEGQQATKILIDQIEGLNQEERQQVLDCSVSWKESTF"
     gene            complement(15372..>16763)
                     /locus_tag="VVN06_000977"
     CDS             complement(15372..>16763)
                     /locus_tag="VVN06_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HEU5562547.1"
                     /translation="KSHYRAKYTLSNWGILQTIPILATYHFFSDKMDLEEAYHFASKE
                     LKQQIETQILEDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSFQELLEKMATYIQMM
                     TGLDGRTLAFGDSDSTETTEILSLSAMVLNKEDLLNGLDVKVDLLSLLFLGREKVKRL
                     QEFEKRAWQPKFRSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPI
                     FIDAGRYSYREVDERYLLKSAWSHSTCIVDGKAPERIVGSWEYEYYPHSLFCHHKERE
                     GVHYIEGAYLSAEPDLPYLHRRKILMLAEDVWLLVDDIRCQGQHEVLTQFILDKDVTY
                     QDGKINQLKLWSEVDFDLEATIISPKYNELEKSSKLTKHQFFENQMLDYTIIAHESFE
                     IIRHSVYQTDGHQVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLV
                     YDKINERMIRLQC"
BASE COUNT         4832 a         3803 c         3038 g         5090 t
ORIGIN      
        1 gctacactcg aaaaaaagaa ccacacctac tatgaacaag acccagaaaa agtagcctta
       61 tttcttaaga attttaatag tttaaagcac ctagcacctg tttagattga cgaaacagga
      121 ttcgatactt atttttatcg agaatatggt cgctcattaa aaggtcagtt aagaagaggc
      181 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgag
      241 ttaatcgctc caatgactta cgaagagacg atgacgagcg acttttttga agcttggttt
      301 cagaagtttt tcttaccaac attaaccaca ccatcggtta ttattatgga taatgtaaga
      361 ttccatagac tggggaagct agaactttta tgcgaagagt ttgggcataa acttttacct
      421 tcttcctccc tactcacctg agtacaatcc tattgagaaa acatgggctc acatcaaaaa
      481 gcctcgtaaa aggtattacc aagttgcaat accttttatg aggctctttt gtcttgttct
      541 tgtttcaatt gactatatta tcagcgtaat agtctaatag atagttaaat gtagttggat
      601 ttccactaaa ggttgatgag taagtttttg tatctgaacc tgattggccg caagcagcta
      661 aaagcaaagc agatgtaaaa gtcaggcctg cactaaggat acgcttcttt atgttcgtct
      721 cctttctcct taatagtggg aatttgtaaa gttaattgaa tttcgagaat gaaggtttat
      781 aaaccttggt tataaaaaac aaaggatttc tgccttttat acagtcctcc cttgttttta
      841 tacgatttca attttaaatt tttctgcaaa aaatatttat agtaattcca cacagaaagc
      901 atcccatgga actaagattt gtttttcaaa gacttcttga gctagggtgt tttcaatcaa
      961 gacagatttg acttttcctt ctactgtcaa gtcttgctct tcattggaca agttagccac
     1021 aactaggaag cgacggtcgc catccttacg tatataagca aagaccttat cagccgtatc
     1081 aagcaattca aagtcagctc gaattagcca gctattctcc ttgcgaattt ggaccagttt
     1141 ttgataggta tagaaaatag aatctggatt tgccagcgct tcttggacat tgatcatctc
     1201 gtaatttgga ttaaccgcca accaaggttg acctgttgag aaaccagcat ttttgctctc
     1261 gtcccattgc ataggggtac gggcattgtc acgtccaata acacggatac tgtccatgat
     1321 ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca tagttgagag attcaatatc
     1381 ttctacttga tccagtgttt caaacggata gttggtcatc ccaatctcct caccttggta
     1441 gatataagga gttcctctca taagatgaag caagattgca aaggctttgg cagatttttc
     1501 gcggtattct tggtcatttc cccagattga gacgatacga gggaggtcat gattgttcca
     1561 gaagagggaa ttccaaccgt cctcaactcc taactctgtc tgccatttgt tgaagatttc
     1621 ttttaactta gcgatattca gctctttttg atagtgccat ttaggctgac cttcctgata
     1681 ctgaagaccg atatgttcaa actggaagac catagacaat tcttgcccct ttggatcaga
     1741 gtagagcttg gcaatctctg gcgttgctcc ccaagtctcc cctactgtca agagatcctt
     1801 atctccaaag gtcgcctgat tcatttcctt gagataggga tggagcatag gaccattatt
     1861 gactaccttc tcgtcaggaa ttttgccaat catgtcaata acatccatac ggaaaccacc
     1921 aataccctta tcaatccaga agttcatcat ctcataaatt ttctggcgaa gtttttcatt
     1981 ttcccagttg agatccggct gtttcttgct gaaaaagtgg agatagtatt gacctgactt
     2041 ttcatcgtat tcccaagcag acccactaaa gatagaatct aaatcgttag gctcatcccg
     2101 ccagatatag tagtctcgct cagggctatt aggattttca caggcctcta caaaccaggc
     2161 atgctcatcc gaggtatgat tgaccaccaa gtccatgatg atacggatat cacgcttctt
     2221 agcttctgca atcagctgat ccatgtcctc catggttcca aaaatagccg caatcgcttg
     2281 ataatcagca atatcatagc cattatcatc catagggctg tcataaacgg gagaaagcca
     2341 aattgctgtg attcctagct tggctagata gtccaactta ctggtaattc ctggcaaatc
     2401 gccaattcca tcttcattac tatccataaa actctttgga tagacttgat agactacggc
     2461 attatgccac catttttctt gcatcttctt acctcattat tttaataatc tatagtatat
     2521 gatagcactt tttaaaattt tgtgcaagcg tttgcttaat ctttttaatt tcttttttag
     2581 ctccgtagtt tcctaacttc caatttttta ttataataga gtccagaggt aaaaaaatga
     2641 aaaaacaagc ttttagttct gaacaatatt taaatctaca acgcgaccac atcttagagc
     2701 gcattaacca atttgacggc aagctttacc tagagtttgg tggcaaaatg ttagaagatt
     2761 tccacgctgc tcgtgtcctt cctggttatg agcctgacaa taaaatcaag ctcttgcaag
     2821 agttgaaaga gcaagttgag gttgtgattg ctattaacgc tagcaacata gagcattcca
     2881 aagcacgtgg cgacctaggc atttcttacg accaagaagt tcttcgtttg attgataaat
     2941 tcaatgaatt ggggattttt gtcggctccg ttgtcattac acaatacgct ggtcagcccg
     3001 ctgcagatgc cttccgcaac caacttgaga aaaatggaat tgattcttat cttcattatc
     3061 caatcaaagg ctatccgacg gatatggatc acatcatttc ccctgaaggc atgggcaaaa
     3121 acgactacat caaaaccagt cgcaacttga ttgtcgtaac cgctcctgga cccggttctg
     3181 gaaaattggc aacgtgtatg tccaatatgt accacgacca aatcaatgtc atcaagtctg
     3241 gctacgctaa atttgaaacc ttccctgttt ggaatcttcc ccttcatcat ccagttaact
     3301 tggcttatga agctgccaca gctgaccttg acgatgtcaa catgattgac cccttccatc
     3361 ttcaaaccta tggagaaacc actgtcaact acaaccgtga tatcgaaatc ttcccagtgc
     3421 tcaagcgcat gttggaacgt attctcggaa aatcgccata cgcttcaccg acagatatgg
     3481 gtgtcaacat ggttggtttt gccattacag atgacgaggc tgctgtcgaa gcttctaaac
     3541 aagaaatcat ccgtcgttac tatcaaaccg ttcttgactt caaagctgaa aaagttggcg
     3601 aagctgccgt caagaaaatt gagttgctta tgaacgacct cggcatcaca cctgccgacc
     3661 gtaaggttgc tgtcgttgcg cgccaaaaag cagaagaaac tggtggacca gccctagcct
     3721 ttgaattgcc aaatggggaa atcgtcactg gtaagaactc agaactcttt ggtcctacag
     3781 ccgctgcctt gatcaacgcc atcaaaaaat cagctgacat cgctaaagaa gtaaaactaa
     3841 tcgaacctga agttgttaag ccaatccaag gtcttaaaat cgatcatctc ggtagccgca
     3901 atccacgcct tcattcaaat gaaatcctga ttgcacttgc tatcacagct acagaaaatc
     3961 ctgatgctgc ccgcgctatg gaagaactcg gcaacctcaa aggaagcgaa gcccactcaa
     4021 ccatcatctt gactgatgaa gacaagaatg tccttcgtaa actgggtatc aacgtaacct
     4081 ttgacccata ctaccaatac gaccgcttat atcgtaagta aagatttcaa cctctccagc
     4141 ctcggagagg ttttctctct gtctcaggag caacctttat gatataatga gagaagaaaa
     4201 ctgaaaggat ttaccatgtc aaaagaagtt attgtcgaaa gttttgaact tgaccacacc
     4261 attgtcaaag caccctatgt tcgcttgatt ggggaagaaa caggaccaaa aggagacatc
     4321 atctccaatt atgatattcg cttggtgcaa ccaaacgaag actctatccc taccgccggc
     4381 cttcacacta tcgagcacct cttagccaaa ctcatccgta cccgcattga cggcatgatt
     4441 gactgttcac catttggttg ccgcacaggc ttccacatga ttatgtgggg acgtcacacc
     4501 agtgctaaaa tcgcagctgt tatcaaggat tcgctcaagg aaatcgctga aactactact
     4561 tgggaagatg tcccagggac aaccatcgaa tcttgcggaa actacaagga ccacagcctc
     4621 ttctctgcta aagaatgggc aaaactcatc ttggaacaag ggatttcaga tgatgccttt
     4681 gaacgtcatg tgatttaaaa tcgagtaggg gataatctct agcccctctc acaccaccgt
     4741 acgtgctgtt cggcatacgg cggttcaact aacttttaac gcatgtcgtt caaggtaata
     4801 atccaaacac gaaaccaatc cacgtttttc aaggactggt tttgatatag cacgtttaag
     4861 taccgacttc tgagctacta tagtggattg aaactagaat agtacacttc tactatacga
     4921 catgcccgtc gtacctcaaa aggaaccagt tttttcagct ggctccttct tttttatcct
     4981 caaaattctg tcttaggctt tctcacgaat aaccaaaacg ccatctttca tatctgcttc
     5041 cagatgttta gcatctaaat gatccaagtg gaagtctgtc accttatcac gaatttcttg
     5101 ttcaaccacg cgacggagag gacgaacccc catgacttcg tcgtaacctt cttctgtgat
     5161 ataatcttta gccgcttgac tgactaccaa gtcaatgtct ttcttagcca aggtttggtt
     5221 aacttcagcc aacatcaaat ctacaatctt agaaaggtct tccttagtca agtgtgagaa
     5281 ctcgatgact gcattaaagc ggttgaggaa ttctggacgg aagaagggtt tcaaacggtc
     5341 catcaattct ggtttatccg catcttctgt caagttggct tcatagccaa atccagcatt
     5401 tgaggtcgca ataatgacag tgttcttgaa gtttactgta tttccttgac catctgtcaa
     5461 acgaccatca tctagaactt ggaggagaag ggtaataact tgagggtcag ccttttcaat
     5521 ttcatccaag agaatgatag agtatggatt gcgacgaaca cgttctgtta aggtattgct
     5581 attgtcatca taacccacat agcctgctgt tgtaccaatt agcttagaaa cagctgtgcg
     5641 gtcactgtat tcagacatat ccaaacggat aatcgcatcc tgggttccaa acatatcgag
     5701 tgccaattgc ttagcaagct ccgtcttacc aaccccagta gaccctacaa agaggaagtt
     5761 gccgattggg cgatttcctt catcaaaacc agcacggtta cgacggatag cacgagctac
     5821 aacttctacg gccttatctt gaccaatcac cttgtcttgc aagcgatgag ccatatcttt
     5881 caaacgttcg atatctgaag ctcccatttg cgatactggg atacctgtca ttcgttccac
     5941 agattcagcc acatcgttga cacttgcagt cactttcata tcttctgtgt ggttttcgat
     6001 tttcctttcc aattctgcaa tgcgtgtttt atagtttaga gctgcttcaa aatcttctgc
     6061 ttcaactgct ttttcttgct tgtctttttc cgtttcgatt tctcgttcaa cagcatgcac
     6121 atctgttact ggatgttgag ccgccaagtg agcagccgtt acatcgacaa ggtcaatagc
     6181 cttatctggc aagctacgtt gaggaatgta ttgaacagaa taatccaccg ctgctttcaa
     6241 gacttcgtct ggcaagatga cattgtggtg ttgttgatag aggtcacgaa ttccttgaag
     6301 aattttaaaa gtattctctg ccgaaggagc attgaccttc acttcgttga aacgacgagc
     6361 aagagcagca ttcttcaaga tggtgttacg gtattcgtct tgagttgttg ccccaatcac
     6421 tgtcaattct ccacgagaga gagctggctt gagaatatcc gcaagccctt tagaaccact
     6481 gtctccacca gtgctaccag caccaagaat ttggtgaatt tcatcaaaga agaggataat
     6541 attccctgct tctttcactt cattgactaa gttttggaca ttttcttcaa agctaccacg
     6601 gtattgagta ccagcctcaa gacctgagat atcaatagaa ataatttcct tgttcttgat
     6661 agcagcagga acatctccgt tcacaatggc ttgcgctaga ccttcgacaa ctgctgtctt
     6721 accaacacct gcatctccga ccaaaacagg attgttcttg gtgcggcgtg agaggatttc
     6781 agatgtttct tgaatttcct tgtttcgtcc gataacaggg tccaacttgc cctcacgcgc
     6841 ttctgctgtt aagtttcgac ctagttttgc aaggacaccg tcttgtttca tacctgatgc
     6901 ctgttgtggc atttgcacat cagtttctgc atttcctggt aattgaccag tcgcacgata
     6961 gtgagcaaat tcctcaggtg tgacttcgcg tccattaatt aagtaacggc gattttcaga
     7021 actgtatcct cgcataccac ccatcaattg gttaaataaa tcatccatgt tattaaaatt
     7081 attaaagttg ttattcatat tctttacctc tttttgttta cttagttatg attactgata
     7141 ttgactatct ttaacctttg ttttaaaaaa tttagactag ctaaaccgct accctacgta
     7201 ctatttctgg tttttctttt tcaagcagga gtagactatc tttacttctg aagatttcta
     7261 gatgaaacat agacccacct ctttctattt tactttaaat aagtgttcta gtaaggcttt
     7321 catttacctt acgttcataa tatactacat tggtcagaaa aggtcaagag ttttgacttt
     7381 aactgaccaa tattttttta cactcttcga aaacctcttc aaaccacatc agcttctcct
     7441 tatctacaac ctcaaaactg tatattaaaa tgaaataaga acaggacaaa tcgatcagga
     7501 cagtcaaatc gatttctaac aatattttag aagtagatgt gtactattct agtttcaaac
     7561 tactatgttt tgagcagcct aaagttagct ttctagtttg ttctttgatt ttcattgtgt
     7621 atttaaacac aaaaacaccc tgaaacatca gggtgccatt cttacatcaa atacaaaatt
     7681 gcgaaggtca ggagacttgc tagaagccca actccccaaa agaagaagtc aaccttccac
     7741 tcgctccaag gatttccttt accagacaat cccccaagct caaaatggtg atgtacaggc
     7801 gtcatacgga aaatacgttt accacctgtc agtttgaaat agctgacttg catcatgacc
     7861 gaagttgttt caaaaacata cacaattccg ataatcaaga gagtccattc ttggtggagg
     7921 gccatagaga tagctgccag cattccaccg agagccaaac ttcccacatc tcccataaag
     7981 accttggcag gcttgtggtt aaagacgaag aaaccaagca aaccaccaat catggcaaaa
     8041 atcaccagga gaatatccat ctggccttgc acataggcaa taactccata ggcagacaaa
     8101 ctaatcacaa cggaaatact agctagaccg tcaataccat ctgtcaagtt gactgcgttt
     8161 gaaaaaccaa ctagccagaa aagagcgaag ataatataga aaatccctaa atgcacttgg
     8221 tagccaaaga cagagagcat atcgccccca cgctcataaa aaaggtagaa aatgacgcca
     8281 cctagcagct gaagagccaa tttctgtttg gggtttagcc cctcattgat ctttcggaag
     8341 accttgagaa agtcatctaa aaaaccaacc aagccataca agaccaaaat aaacaaaatc
     8401 ataccaacat tattggtcaa ttgtttcgaa aaaagagcga cgaggaaact caccacaact
     8461 gcagcaatga ggaaaacaag tccccccatt gtaggagtcc cagcttttgc ctggtgctgt
     8521 ttaacatcct catgcatctg ctggcctgta atttgcgcct ttctataaaa ttggataaag
     8581 gccggaattc ctactaaagt tagtaaaaat gtcacaattc cagcactaat agaaataaac
     8641 atattagtct cctaaagtta atttaatttt tttaatgttt ttgatagctg tattagtccg
     8701 aacatcttgc ttctgaacga cggaacctga accttcaaat tctagctcaa tatccaacca
     8761 tttagcaaaa gtctcagcag tctccttttt ccagccatac atgtctggaa tttcctctac
     8821 cttatccgat aaaaggagaa cttgttggtt tggtgcaaga ttggtccctt cttctacaga
     8881 agtctcttta atctttgttc cagtacctac aacgattggt tgcacaatat ttcggcgtaa
     8941 ggcttccgcc aactcaccag gtgaaatatc cttgatgcta ggcattgcat aagaagattc
     9001 tgtcgtaact ttatctaaat ttttggctgg agattgaaga ttgagagatt ctttcatagc
     9061 tgaagcccgc tccaagattg gggtggcaaa ttctcccaac tggatacctg aataatgctc
     9121 aggctgttga accgttacat acaagataaa atcaggattt tcagcaggat tcatagtcac
     9181 agctgagaaa atataattgg tagaaccaac caagtatcct ccatttttct catcagcgat
     9241 ttgagccgta ccggatttaa ctgctacatt ttgtccagga actgttataa ttggctttcc
     9301 tgtgtagtga ttatacatag ttccatatag agggtccgtc ccaactaaga tcatgtgatt
     9361 tcgagttgtg cttgctgtat cttcagatac aggttttcct acaatctctt tttgagactt
     9421 tcgtacagac tgattgttag tatcataaat agcacttata aattttggct ccagcataac
     9481 tccatcatta gcaatagctg taaaggcacg aagcatttgt gtttgtgtca ctgaaattcc
     9541 ttgcccaaat gagctttgag caatactaac aatattatca gctggaagtt gaccagcgta
     9601 ttcatctgtc aagccaaagc gagttggaac cccaaattta aagcgtttta gataatccaa
     9661 ccaagtagca tctcccattt tttgttcaag tagactcatt ccaacattac tggagtgagc
     9721 gaaaccttgt aagaaagtca tcatcccacc agtagtcaaa ccctcattaa catcccaatc
     9781 tcgagtcgtc gcatccgcta ttttaaattc actgctattg aagtattctc cacttgggaa
     9841 ggtattatta tcaatagaag aagctaacgt cataaccttc atggctgatc ctggttcata
     9901 gttactttga taaagaatat cacgccaaac aaagtcctca gtgattcctt ctttagtatc
     9961 tgcattaaag gtaggtcgtt gggtggtagc gaggatttca ccggtctttg cactgaccaa
    10021 ggtcgcggtc atatacttac cttttacttt ttcttgaaag gcattcatct gtgtctccat
    10081 gaaggactga agggtgctgg aaatagtcgt ataaacatcc ttgccatcta ctgtttgttg
    10141 ggaaacttgt tctgttccgg ggacaatatt acccagacga tccttttcat aggtaatgat
    10201 accgtctttc cctgcaagaa tactattcaa ggaactctcc atcccagaag ttcccagcaa
    10261 actcttgcta ccatcctcat tttcatggag ttgggctagt ccgataaaac tagaagcaaa
    10321 ttgtccgttt ggataactac gattgggact ggttgtaaaa tcgatcccct ttacctctgc
    10381 agtttccaac tcttttttga tagccatcat attggcatag gtaatcccat tgcccttcga
    10441 accaaaggaa acttgcttga gcttaggttg cgagagttgc tctcttacgt aggattcttc
    10501 catgtccaga tacttatgaa agacctctgc aaccttgtta aattgtgttt tttctacgta
    10561 aagaatctta cccgttgctg acttatagtt ctcatcaatg accgcataga cattatagga
    10621 ggttgcatcc tcagcaatcg ggactccatt tcggtcataa atagtcccac gtttggcagg
    10681 aactgtacgg gtggtttgat gaaccttctt agcttccttc gctaaatctg ttccaaagcg
    10741 agtgcccgtc ccaataatga ccgcaaaatt gactaaaaaa acggcaaaaa caaagacaga
    10801 taataaactc agactttttc caactctgcg tctgttttca gccggcgatt tccgattttt
    10861 cgtcgcataa cggattactt tttttgtcca cttcatatct tactccgcta ttcgaatatt
    10921 ttcattgttt aattgcaaat cgtgtgaatt ggcaatttct ttcaaacgtt ctgcacgtaa
    10981 tagttcattg acctcttgct tggcatcgtc caattcggtc ttcttttcct ctatctgcgc
    11041 attgattttt gtcaaatcat tctgcacttg caagagctta gtctgcataa aaataatact
    11101 aatggctaca ataagagtgg ttacagcaat ggaaaagtaa aaagcttttt ccacacgcga
    11161 aaaccgttta agttgcatct gtagtatttg acctgttttt tccattcttt ctgccatcct
    11221 ttttccctct tacttgtgaa tttttctggc cacgcgcaac ttggctgagt gcgagcggtt
    11281 attggcttct aattcttctg cacttggcaa gattggctta cgggacacca attccatctt
    11341 gggcttgaga tcatctggga tgaaaggcaa acctttggga acttcaaccg ttgaagcttc
    11401 cttgaacaat tgcttggtca agcgatcttc taaggaatga aaggtaatca ctgaaattct
    11461 accatccaga gccaacatat ccatagcctg ctggatggac tcatctgccg ctcccagttc
    11521 atcattgact tcaattcgaa tagcctggaa aatctgctta gcaggatgac ccttcttctt
    11581 aagttccttg gcaggtttag ccgacttgat aatctctgct aactcagtcg ttgtctcgat
    11641 tggtttgact tcacgtgctt gctcaatctt acgcgcaatc tgtttagaga atttgtcctc
    11701 accatacttg aagaaaatac gaactaagtc atgatagtca tagtggttca ccacttcata
    11761 ggctgtcaga ctagcatcct gattcatccg catgtccagt ggcgcatcct ttttataaga
    11821 aaaaccacgc tcacgctggt ccaattgagg actagacact cccaagtcat aacaaattcc
    11881 atcaatttcc tgaacaccag cttcgcgcaa acatgcctgt aaatgacgga agttatcctt
    11941 gataaaggtc accattccct tttcaatgta aggtgccaag cgtttttgcg cattgtcaat
    12001 ggcattctgg tcctggtcaa aggcatagag atgacctttt tcacttaact tacttaataa
    12061 atattcgcta tggcccgctc cacccaaagt cgcatcaacg tagataccgt caggctttac
    12121 gtcgagcata tcaatcgttt cgtggagtaa gaccgttaca tgatgaaatt cttttgtcat
    12181 atcttatcta ttttaccaca aatctgacta gcttgcacta gtcagacaaa tagggcaaaa
    12241 aaagtaagat ttctttaaga aatatgtcaa atatgcttga cattcactct atagaagaat
    12301 ataatgtagt caaatatata cgacatgaat cagaaaggag tccagatgaa tcgtgtgaaa
    12361 gaatttcgca aggaattggg tatttcccag ctcgagctag ccaaggatat cggtgtctcg
    12421 agacaaacca tcaatatgat cgagaacgac aagtacaacc caactctgga actctgtctc
    12481 aatctcgccc gcagcctcca aactgacctc aatagtctct tttgggagga taatttttaa
    12541 aaaaggagcc aactcatgaa aaaagaaacc ttcactgaaa aactcatcaa acgcatatac
    12601 ggaatttctg gccctctcga cgaacacaaa cgacgcgagg ctgatcgtat cggcaaccaa
    12661 gtctttatca tcctctttta tctgatgatt ttcggaaatc ttattccact ccttctggcc
    12721 tataaatacc ctcaagaagt ggctctaatc tatcctcctc taattttagt gattgccctc
    12781 atcgctgctg gctatgtcac ctacaaaatg aaaaaaacag gaattacagc tattgatcca
    12841 gatatgctga gcgagaaaga aagtaagcaa ctatactatc caggtcttaa agcaggtttg
    12901 ttctttggtc tatggatgtt ttttataact cctcttctaa gtatactcat aggtgaaggt
    12961 caggactatt ttcattctct tctcactata agaaatggtg tatcaagcat tctcggttct
    13021 attttcttcg gagcgagcat acagtttctc atctcccgtc gcattgcaaa agctaaggaa
    13081 gatcaggatg aggattagga ggtgccttat gaaatcatta ctaactttac ttacctatca
    13141 tcttttgttc agttctctgc tcatcttcat catcatttca gggaacttgc caatcgacga
    13201 gacattcctc gtgctagtct ttattctagc ccttaacaaa ggactgactt atctaaagat
    13261 tgactcccca aaaacgcgca cacttaactt agcactatac tttatgattc tatcttttcc
    13321 acaactgatg ctcatatcaa gcgattggaa atattattta ctgctgacta ttgctagcct
    13381 ttcttctatc acttatcttt attatctcta tcaattcgta aaagaaagtc agtaaaactg
    13441 ctcatttagg aggttattag catgaacaaa gaagacttaa ccactcgcct gcttcgaaat
    13501 ctctttcata ttcagggtcc ttttgatgaa tgccgacaag agattatcta caaagcttgt
    13561 gcccgttcta tggttcaaat cgtttattct tccttcttcc tcttcttgtt ttatctatta
    13621 tttggacgct tcatagaagc cgttcgctat gccatgccct acgtatactc tggactcatt
    13681 tttttcatta ccttaaaaac tcaaagagcc gtcaaagaac tccacttgga aaaagatgac
    13741 aagtcagaaa tcatcctcaa aacctacagc aaagcccaaa tcaaattccg aagctgggtt
    13801 gtgtttatcg gtattcagat tggcctcttt accctactca tctttcataa agtcttcgtt
    13861 cagcagatgt ccttttcaga ttttggaaag ttgctcatgc aatttgataa aagtggtcct
    13921 ttcctgatgt atggcctgat tatcggtagt attttcggaa ccctgaccta cggatttcta
    13981 tcacttcagg aggagaaaac tcctaaaaat accaaacaga aggagaaaag taagcaatga
    14041 cttcactata cgatttttca gtcttgaacc aagaaaatca agaaactcca tccaatggaa
    14101 atttccaata ttcctagtcg taaccatcat ctcaagtttg gtctacttct ataactttta
    14161 tcaagtagtt aaagaagtaa atcaaaaata gttgatttag gagattactc ccgtaatttc
    14221 tcattttata gacaaataat cccatccagt tcatgctaga tgggattatt ttatttctta
    14281 acagaggttg ccagttattt ccctttcatc ttagaaagtc gactctttcc aactcacact
    14341 acaatccaag acttgttgcc tttcttcttg attgaggcct tcaatctggt caatcaaaat
    14401 ctttgtagcc tgttgtcctt cttcaaaggc gggctgaacc aacgtcgaaa cacttggaga
    14461 agaaaagcaa gtccactccg tattgtcaaa accaatcaac ccaacttgtg gcaagttata
    14521 attcaactct ttgataacgg taaagactgg aggtagggcc caacagttag gaacaaatac
    14581 cagagttttt tcatcgggat tgatttcttt ttgtaaaaat tccttaattt gttccaaatt
    14641 cgtatgctta tcttcaatgg ttagcctagc gtggcgcata ttagcatctg ttaaagcatc
    14701 cacaaaacca cttgcccgct caatccgagt actcaaacga ctcgtatccg ctgtaatcaa
    14761 gagaaaatgc tcataacctt tttcgataca ggactgggtc atatcataaa cggcatcata
    14821 gttattggtt ttgacccagc tagtccggtg ttcatagagc cgactatcaa aaaagaccat
    14881 tttcttcttt ttctcatcga tgatacgaga atattttcgg aaattagagg taggctgaat
    14941 aataaagccg tctactccca agagaagcat gctttcaata tacctgtcct cactctcttg
    15001 gctgtagtta ctatttccta tcattacctg gtagccattc tggctggcga tatcctcaat
    15061 tcccttaaca atttgggttg agaaactgtt ggtaatatca ccaatcaaaa caccaattaa
    15121 ttttgttcgt ttggagttta agctacgcgc aacaatgctc ggtttgtaat ttgtttcatg
    15181 aataactttt tcaatctttt cacgtgtctc ttgggacatt ttttcatatt tcccgtttag
    15241 gtaaaatgac acggttgttt tcgaggtctg agccatttct gcaatatctt ttatggtcag
    15301 tttcttctcc aatctaaaac acctcctact aatacccatt ataacatatt cactattcaa
    15361 aacgtctatt tctagcactg caagcgaatc attctctcat ttattttatc atatactaga
    15421 cattttccac gcatttttgt tccgtcaacg aggcataatt tttcacctac acaaatatcc
    15481 tcgcttaata acagaatcag cttgtctgtt tcgtcatttt tcacttcaaa agccagagca
    15541 ttttctactt gatgaccatc tgtctggtag acagaatgac ggatgatttc aaaactctca
    15601 tgcgcaatga ttgtataatc cagcatctga ttctcaaaga attggtgttt ggtgagtttg
    15661 ctactttttt caagctcgtt gtatttagga gaaatgatcg tagcttccaa atcaaaatca
    15721 acttcactcc atagtttcaa ctgattgatt ttcccatctt gataggtcac atccttgtca
    15781 aggataaact gagtcaacac ctcatgctga ccttgacacc tgatatcatc taccaagagc
    15841 catacatcct ctgccaacat gaggattttt ctcctgtgaa gataaggcaa atcaggttct
    15901 gctgacaaat aagccccctc aatataatgc actccctctc tttctttgtg gtggcagaac
    15961 agggagtgag gatagtattc atattcccag gatcccacga tcctttctgg agctttccca
    16021 tctactatac aggtcgaatg actccaagca ctctttaaga gataacgttc atcaacctcc
    16081 cgataagaat aacgcccagc atctatgaaa atcggttggc cttgatactg taagcaaaaa
    16141 ctattctcgt cactatggct atgggcactt cccagcggac cgtttttaaa aaatagataa
    16201 cgatgttcat ccttaatgca aacatgccca gaatcttcaa agctcctgaa cttaggctgc
    16261 caagctctct tttcaaattc ctgcagtcgc ttgacctttt ctcgccccag gaacaagagg
    16321 ctaagtaaat cgactttaac atccagaccg ttaagaaggt cttccttgtt caaaaccata
    16381 gcagacaggc tcaaaatctc tgtcgtttct gtagaatcgc tatcaccaaa agccaaggtc
    16441 cgtccatcta gacctgtcat catttgaatg taagtcgcca tcttttccag cagctcttga
    16501 aagctatctt gcaagtccgg aagcaagaga cacaagtcca gcaaggcttt ataaacctct
    16561 acatgataga gaatcgactg ttcaaactgg cttccatctt ctaaaatctg cgtctcgatc
    16621 tgctgtttca actcctttga agcaaaatgg taagcttctt ctaggtccat cttatctgaa
    16681 aagaaatgat aggttgcaag tatcggaatc gtttgtaaaa tcccccagtt actaagggtg
    16741 tacttggcgc gataatggct ttt
//
LOCUS       DAVHIU010000035        16372 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVHIU010000035 DAVHIU010000000
VERSION     DAVHIU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.037"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(114..824)
                     /gene="treR"
                     /locus_tag="VVN06_000978"
     CDS             complement(114..824)
                     /gene="treR"
                     /locus_tag="VVN06_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU5562548.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1009..2976
                     /gene="treP"
                     /locus_tag="VVN06_000979"
     CDS             1009..2976
                     /gene="treP"
                     /locus_tag="VVN06_000979"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU5562549.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            3154..4779
                     /gene="treC"
                     /locus_tag="VVN06_000980"
     CDS             3154..4779
                     /gene="treC"
                     /locus_tag="VVN06_000980"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU5562550.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5035..5283
                     /locus_tag="VVN06_000981"
     CDS             5035..5283
                     /locus_tag="VVN06_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU5562551.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5490..6284
                     /gene="racE"
                     /locus_tag="VVN06_000982"
     CDS             5490..6284
                     /gene="racE"
                     /locus_tag="VVN06_000982"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5562552.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6281..7252
                     /locus_tag="VVN06_000983"
     CDS             6281..7252
                     /locus_tag="VVN06_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU5562553.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7228..7749
                     /locus_tag="VVN06_000984"
     CDS             7228..7749
                     /locus_tag="VVN06_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5562554.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            7746..8207
                     /locus_tag="VVN06_000985"
     CDS             7746..8207
                     /locus_tag="VVN06_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5562555.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDSEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8198..8938
                     /gene="xerD"
                     /locus_tag="VVN06_000986"
     CDS             8198..8938
                     /gene="xerD"
                     /locus_tag="VVN06_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU5562556.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISSCNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            8938..9666
                     /locus_tag="VVN06_000987"
     CDS             8938..9666
                     /locus_tag="VVN06_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU5562557.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLESKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            9685..10254
                     /locus_tag="VVN06_000988"
     CDS             9685..10254
                     /locus_tag="VVN06_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU5562558.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10241..10963
                     /locus_tag="VVN06_000989"
     CDS             10241..10963
                     /locus_tag="VVN06_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5562559.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            10963..11205
                     /gene="yidD"
                     /locus_tag="VVN06_000990"
     CDS             10963..11205
                     /gene="yidD"
                     /locus_tag="VVN06_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5562560.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(11430..12395)
                     /locus_tag="VVN06_000991"
     CDS             complement(11430..12395)
                     /locus_tag="VVN06_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562561.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFAKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(12491..13243)
                     /locus_tag="VVN06_000992"
     CDS             complement(12491..13243)
                     /locus_tag="VVN06_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5562562.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(13240..14196)
                     /locus_tag="VVN06_000993"
     CDS             complement(13240..14196)
                     /locus_tag="VVN06_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU5562563.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(14186..15145)
                     /locus_tag="VVN06_000994"
     CDS             complement(14186..15145)
                     /locus_tag="VVN06_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562564.1"
                     /translation="MKLSYYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKCYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(15442..15594)
                     /locus_tag="VVN06_000995"
     CDS             complement(15442..15594)
                     /locus_tag="VVN06_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEU5562565.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFNLGTKKGYTIKEIFKT
                     AEELLN"
     gene            complement(15591..16310)
                     /gene="galE"
                     /locus_tag="VVN06_000996"
     CDS             complement(15591..16310)
                     /gene="galE"
                     /locus_tag="VVN06_000996"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5562566.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGRNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTH
                     DGSCIRDYIYVLDLHRLMLKE"
BASE COUNT         4598 a         3263 c         3774 g         4737 t
ORIGIN      
        1 tcttatggga ctttttttct acaataaaat aggctccata atatctatag ggggggttac
       61 ccactacaaa tattatagag ccgaaaaagg ctgagatctg gtgtctcagt cttttatttc
      121 ttgcgttttg caaaatctac aaatctaaat ttctctaact tatggcgact ttcggtaaac
      181 tgaaattggc gtccgtcttg gagatagact tttgatttaa tcgaaacgac ataagggtct
      241 ttgccaatgt ccatgagaat cttgtctcgg tcgcttgagt ggtcaatggt gatttccttc
      301 tgagcataat caataaggag tttgaggcca ttttctatat aagaatagat agactgctca
      361 gcaatttggc gagtgagatt tgggatgagt tccatatcca gatagtctgt atccagaacg
      421 gataccagat catccaccac acgctggcgg accaccttcc aaaccatccg aaactctggg
      481 aaaccggtta tcagtgagga ttttttatca ataataatct tgtccagact gaccacgttg
      541 gttttagagc gcagtccaag ttctttaact agttcttggt agctggttag gttggataca
      601 gggaaattga cggtttcttc tttgacgact tgagaacctt gccctcttat ctttttgatc
      661 aatccttcct cttggaggag agacagggcc tttcggacgg tatcacgact cacttgatat
      721 tgctccataa ggtcgtgctc gctaggaagg aaatctccga cagcgtaagt ctcgttttga
      781 atggtttctt ggatttgctt aaataattgt tggtatttct tcattttgcc tgcttaaata
      841 ggtttttagt ctagtttaat gaacacctgt atctatttta ccatacaagt gctagaaaat
      901 ttacaaaaaa acaccctttt tataccaaac aagttgcaga caagtttggt atcgtttaca
      961 atatacttat caaatcaaac ttgctttgac aagtataagg agaatcaaat gggaaaattt
     1021 gaacaagaag ccaaagatct gcttcaggca attggaggca aagaaaatgt gactgccgta
     1081 actcactgtg cgacacggat gcgctttgtt ttaggagatg ataagaaggc taatgttaaa
     1141 gctatcgagt caattccagc tgttaaagga acttttacaa atgcaggtca atttcaggta
     1201 atcattggaa atgacgtgcc catcttttat aatgatttta cagccgtttc aggtattgag
     1261 ggtgtttcta aagaagcagc caagtctgca gctaagagta atcaaaacgt ggtccaacgt
     1321 gttatgacca ctctggcgga aatctttact ccgattattc cagctttgat tgttggtgga
     1381 ttgatcctcg gtttccgtaa tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag
     1441 accatcacag aatcctctca gttttgggca ggggttaatc acttcctctg gttgcctggt
     1501 gaagctatct tccagttctt accagtaggg attacttggt ctgtttctcg taagatggga
     1561 accagccaaa ttttgggaat tgttctcgga atctgtttgg tatcgcctca gttgctcaat
     1621 gcctatgcgg ttgcttcaac gccagcagct gatatcgcag caaactgggt ttggaatttt
     1681 ggttatttta ctgttaatcg tattggttac caagcccaag ttatcccagc cttgcttgca
     1741 ggtttgagtc tgtcttatct tgaaattttc tggcgcaaac atatcccaga agtcatttcc
     1801 atgatttttg tacctttttt gtcattgatt ccagccttga ttttggctca tactgttttg
     1861 ggaccaatcg gttggacaat tggacaagga ctttcatcag ttgtcttggc aggtttaact
     1921 ggtccagtta aatggctctt cggtgcaatt tttggtgccc tctacgctcc atttgtcatc
     1981 acaggtctgc accatatgac caatgccatt gatacacaat tgattgcgga tgctggtggc
     2041 actgccctct ggccaatgat tgctctttct aatattgctc aaggctcagc cgtgtttgcc
     2101 tattatttca tgcatcgcca tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt
     2161 tcagcctatc tcggtgttac agaaccagct ctttttgggg ttaacgtaaa atatatttat
     2221 ccatttgttg ctgggatgac tggttcagcc cttgcaggca tgttatccgt tacttttaat
     2281 gtaactgcgg cttctattgg tatcggtggt ttgccaggta ttctctctat tcaacctcaa
     2341 tacatgctgc catttgcagg aactatgcta gttgcgattg ttgttccaat gctcttgact
     2401 ttcttcttcc gcaaggctgg tctctttaca aaaacagagg gcgatacgaa cttgcaggca
     2461 gaattcgttg ctcaagaaga agcagaattt gtgaaccatg aaccagtaga acttacttcg
     2521 gtagaaatta tcagcccact aactggccaa gtgaaagaat tgagtcaagc gacggatcct
     2581 atttttgcat caggtgtcat ggggcaaggt ctggtcattg aaccaagcca aggtgagttg
     2641 acctctccag ttaatgggac agtgacggtt cttttcccta ccaagcatgc catcggcatt
     2701 gtctctgacg agggagttga attgctcatc cacatcggta tggatacagt aggtcttgat
     2761 ggcaaaggtt ttgaaagtct tgtagtccaa ggagatcacg ttacagttgg tcagcaactg
     2821 attcgttttg atatggatgt cattaaggct gcaggtctgg tgacagaaac tcctgttatc
     2881 atcaccaacc aagatgctta tacagcgact attcccggaa cttatccgac aacgatccaa
     2941 gctggagcat ctctcatggt cgctacacga atctaatact cttcgaaaat ctcttcaaac
     3001 cacgtcagct ctatctgcaa cctcaaaata gtgttttgag caacctgtgg ctagcttcct
     3061 agtttgttct ttgattttca ttgagtataa ttgaaatagc acaaataggg acggaagact
     3121 gcttcctccc ttttatcaga aaaggagaaa cagatgacac ttgataaagg aaaagtagtc
     3181 tatcaaatct atccaaaatc ttacaaagac accactgaaa acggtattgg ggatttccgt
     3241 gggattatcg aaaaaattcc ctatttagcc aaactaggtg tggatatggt ctggctcaat
     3301 ccattctatc caagccctca acgggataat ggttacgata tttcagatta tatggcagtg
     3361 gatcctcttt ttggtgatat ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac
     3421 aagattgact ttatgctgga catggtactt aatcattgtt cgacagagca tgaatggttt
     3481 cagaaagccc tagctggtga taagtattat caagactttt tcttcatcca agaccaacca
     3541 acagattggc agtctaagtt tggtggttct gcatggacgc ctttcgggga tactgggaaa
     3601 tactatcttc acctatttga tgagacccag gctgacctta actggcgcaa ttccaatgtc
     3661 cgtaaggagc ttttcaaggt tgttaatttc tggcgcgaca agggtatcaa aggtttccga
     3721 tttgatgtga tcaatttgat cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa
     3781 gggaagccag cttatacaga caaacccatc gttcataact atttgcgtat gatgaaccaa
     3841 gccacttttg gatctgacga tagctttatg acagttgggg aaatgtcttc taccaccatg
     3901 gaaaactgtg tcctttattc gtcgcctgat cgtcaggaat tatccatgac ctttaatttt
     3961 catcacctca aggtggacta taaggatgga caaaagtgga ccttggctcc ctttgatttt
     4021 gaagagttga agagtcttta ccatagttgg ggtaaggaaa tgagtgataa agacggttgg
     4081 agtgccctct tttggaacaa tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt
     4141 caaaacttcc gcaaggaagg agccaccatg ctagcggcca gcattcacct gtcacgtggg
     4201 acaccttata tctacatggg tgaggaaatc gggatgattg acccagacta tgattctatg
     4261 gctgattatg tggatgtcga atcgcttaat gcctatcaga tgctcttaga agaaggaaag
     4321 agtcagcaag aagccttcca gattattcaa gctaagtcgc gtgataattc acgcattccc
     4381 atgcagtggg atgcttcgga aaatgcagga ttttcaacag gcactccttg gttgaaagct
     4441 ggtaaatcct ataaagacat caatgtagaa aatgaaatcc agggtccgat ttttaccttc
     4501 tatcaagact tgattcgact gcgtaaggaa atgccaatca tttcagaagg aagttacaaa
     4561 ccagcctttg aagatagcaa acaagtctac gcttttgaac gccagtttga ggatcaaaag
     4621 ttactagtgc tcaataactt ttatgctaaa gaagtggaaa tcgacttgcc agcagtctac
     4681 caaaatggac agattttgat atcaaactat gaagatgcag aagtgtctga gaaaattcta
     4741 ctcaaaccct atcaaacact ggctatttat gtaaattaat actcttcgca aatctcttca
     4801 aaccgtgtca gttttatctg caacctcaaa gcggtgcttt gagcaacctg tggctagctt
     4861 cctagtttgc tctttgattt tcattgagta taaacttagc aaggagacct ctttttcaga
     4921 aaagcagggt tctttttgct gtctattcca tccttgaaag tgtagaacaa actggtcagg
     4981 gtggctagtt tgtggtataa tgaaagggat tcaaaaagaa acaggagaaa aatgatggat
     5041 ttacttttag caattgtatt gattgtgcta gcttttctag gaggagctct tggaggaatg
     5101 tacttggttc gtaagcaaat cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa
     5161 gcagttcgta ctcttttgag tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa
     5221 gtttaccacc aaatcatccg ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa
     5281 taaataagga aaagtctggg atgaaagttc cagacttctc actatgttgg ttatggttta
     5341 gggatgagac tttttccttg cattctattg atatttgatt atgattaaga gagaggtagt
     5401 tgacattcta tcaagctgtc ggtcagttcg ttcagtacaa ggaatcataa tgaacgatca
     5461 atcagaaaaa agactagaaa gaagactgta tggataatcg accaattggt tttttggatt
     5521 cgggtgtcgg gggcttgacc gttgtgcgcg agctcatgcg ccagcttccc catgaagaaa
     5581 tcgtctatat tggagattcg gcgcgggcgc cctatggccc ccgtcctgct gagcaaattc
     5641 gtgaatatac ttggcagctg gtcaactttc tcttgaccaa ggatgtcaaa atgattgtca
     5701 ttgcttgtaa cactgcgact gcggtcgtct gggaagaaat caaggctcaa ctagatattc
     5761 ctgtcttggg tgtaattttg ccaggagctt cggcagccat caagtccagt caaggtggga
     5821 aaatcggagt gattggaacg cccatgacgg tacaatcaga catataccgt cagaaaatcc
     5881 atgatctgga tcccgactta caggtggaga gcttggcctg tcccaagttt gctcccttgg
     5941 ttgagtcagg tgccctgtca accagtgtta ccaagaaggt ggtctatgaa accctgcgtc
     6001 ccttggttgg aaaggtggat agcctgattt tgggctgtac tcattatcca ctccttcgcc
     6061 ctattatcca aaatgtgatg gggccaaagg ttcagctcat cgatagtggg gcagagtgcg
     6121 tacgggatat ctcagtctta ctcaattatt ttgaaatcaa tcgtggtcgc gatgctggac
     6181 cactccatca ccgtttttac acaacagcca gtagccaaag ttttgcacaa attggtgaag
     6241 aatggctgga aaaagagatt catgtggagc atgtagaatt atgacaaata aaatttatga
     6301 atataaggat gaccaggact ggtatgttgg gtcttatagt atttttggtg gcgttaacag
     6361 tttgagcgac tataagacag attttcctct gtttgaattc tccaaaatat ttggagatga
     6421 agagtatggt ttcccgcttt cagttactgt tttacgctat ggttctacct accgtttgtt
     6481 ctcctttgtg gtagacatgc ttaatcaaga aatgggacga aacttggaag ttattcaacg
     6541 tcatggggcc ctgctcttgg ttgaaaatgg gcaactcttg tatgtagaat tgcctaaaga
     6601 aggggtcaat gttcatgatt tctttgagac aagcaaggtc agagaaacct tgttgattgc
     6661 gactcgtaac gaaggtaaaa ccaaggaatt ccgagctatc tttgataagt taggctacga
     6721 tgtggaaaat cttaatgact accctgacct gcctgaagta gcagaaacag gtatgacctt
     6781 tgaagaaaat gcccgcctta aggcagaaac catttctcaa ttaacgggca agatggtttt
     6841 ggcagatgat tctggtctca aagtcgatgt ccttggtggc ttaccaggcg tctggtcagc
     6901 tcgtttcgca ggtgtgggag caactgaccg tgaaaataat gccaaactct tgcacgaatt
     6961 ggccatggtc tttgaactca aggaccgctc ggctcagttc cacacaaccc tagtcgtagc
     7021 cagcccaaat aaggaaagtt tagttgttga agcagactgg tcaggttata ttaactttga
     7081 acctaagggt gaaaatggct ttggctatga tcccctcttc cttgtaggag agacaggtga
     7141 gtcatcagct gaattaaccc tggaagaaaa aaatagtcaa tctcaccgtg ccttagccgt
     7201 taagaaactt ttggaggtat ttccatcatg gcaaagcaaa ccatcattgt aatgagcgat
     7261 tcccatggcg atagcttgat tgtggaagaa gtccgtgatc gctatgtggg caaagtcgat
     7321 gccgtttttc ataacggcga ttctgaacta cgtccggatt ctccactttg ggagggcatc
     7381 cgcgttgtta aagggaacat ggacttctac gccggctacc cagaacgtct ggtgactgag
     7441 cttggttcga ccaagattat ccaaactcat ggtcacttgt ttgacatcaa tttcaacttt
     7501 caaaagttgg actactgggc tcaggaggaa gaggccgcta tctgcctcta tggtcacttg
     7561 catgtgccaa gtgcttggat ggaaggcaag atcctctttc taaatccagg ttctatcagt
     7621 caaccacgag gtaccatcag agaatgtctc tatgctcgtg tggagattga tgatagttac
     7681 ttcaaagtgg actttttgac acgagatcac gaggtgtatc caggtttgtc caaggagttt
     7741 agccgatgat tgccaaggag tttgagactt tcttgttggg gcaggaggaa acttttttga
     7801 cccctgctaa aaatctagct gtgttgattg atacccacaa tgcggatcat gcgaccctct
     7861 tgctcagtca gatgacctat acccgtgttc ccgttgtgac agatgaaaaa cagtttgttg
     7921 ggacgattgg actcagagat attatggctt atcagatgga gcatgacttg agccaagaaa
     7981 tcatggcgga tacggatatc gttcatatga caaaaacgga cgtagcggtt gtttcgcctg
     8041 atttcaccat tacggaggtc ttgcacaagc tagtagatga gtccttctta ccggttgtgg
     8101 attcagaggg tattttccaa gggattatta cgcgcaagtc catcctcaag gccgttaatg
     8161 ccctcttgca tgactttagt aaggaatatg agattcgatg ccaatgagag acaggatttc
     8221 agccttttta gaggaaaagc agggcttgtc tgtcaattcc aagcagtcct ataagtatga
     8281 tttggagcaa tttttagaca tggtaggtga gcggatttct gagaccagtc tcaagattta
     8341 ccaagcccag ctagccaatc taaaaatcag cgcccagaag cgaaagattt cgtcctgtaa
     8401 ccaatttcta tactttctct atcaaaaagg agaggtggac agcttttacc gcttggaatt
     8461 agccaaacaa gctgaaaaga agacggaaaa gccagagatt ctatacctag actctttttg
     8521 gcaggaaagc gaccatccag agggccgctt gctagcgctc ttaatcctag aaatggggct
     8581 cttgcccagt gagattttag ccatcaaggt tgcggacatc aatctggatt ttcaggtgtt
     8641 gcgaatcagc aaggcttccc aacagaggat tgtcaccatt cccacggcct tgctttcaga
     8701 attggaaccc ttgatggggc agacctatct ttttgaaaga ggagagaaac cctattctcg
     8761 tcagtgggcc tttcgtcagt tagaatcttt tgtcaaggag aaaggttttc catccttatc
     8821 agctcaagtc ttacgtgaac agtttattct aagacaaata gaaaacaagg tcgatttgta
     8881 cgaaattgca aaaaaattag gattaaaaac agtcctgacc ttagaaaaat atagataatg
     8941 gatattaaat taaaagattt tgaaggaccc ttggacttgc tcttgcatct ggtttctaag
     9001 taccagatgg atatctacga tgtgcccatt acggaagtca tcgaacagta tctagcctat
     9061 gtctcaaccc tgcaggccat gcgtctggaa gtgacgggtg agtacatggt catggctagt
     9121 cagctcatgc tgattaagag tcgtaaactc cttccgaagg tagcagaagt gacagacttg
     9181 ggggatgacc tggagcagga cctcctctct caaatcgaag aatatcgcaa gttcaagctc
     9241 ttgggtgagc acttggaatc caagcaccaa gaacgggccc agtattattc caaagcgccg
     9301 acagagttga tttacgaaga tgcggagctt gtgcatgaca agacgaccat tgacctcttt
     9361 ttggcttttt caaatatcct agccaagaaa aaagaggagt ttgcacaaaa tcacacgact
     9421 atcttgcggg atgagtataa gattgaggac atgatgatta tcgtgaaaga gtccttgatt
     9481 ggacgagatc aattgcgctt gcaggatttg ttcaaggaag cccagaatgt ccaagaggtc
     9541 atcaccctct ttttggcaac cctagagtta atcaaaaccc aggagctgat cctcgtgcaa
     9601 gaggagagtt tcggagatat ctatctcatg gaaaagaagg aagaaagtca agtgcctcaa
     9661 agctagactt gatagagagg aaagatgagt actttagcaa aaatagaagc gctcttgttt
     9721 gtagcgggtg aagatgggat tcgggtccgc cagttagctg aactcctctc tctgccaccg
     9781 acaggcatcc agcaaagttt aggaaaatta gcccagaagt atgaaaagga cccagattcc
     9841 agtttggctt tgattgagac aagtggtgct tatagattgg tgaccaagcc tcaatttgca
     9901 gagattttga aggaatactc taaggcgcct atcaaccaga gcttgtctcg ggctgccctt
     9961 gagaccttgt ccattattgc ctacaaacag ccgattacgc ggatagaaat tgatgccatc
    10021 cgtggagtta actcgagtgg agccttggca aagttgcagg cttttgacct gataaaggaa
    10081 gacgggaaaa aggaagtatt ggggcgcccc aacctctatg tgactacgga ttatttccta
    10141 gattacatgg ggataaacca tttagaagaa ttaccagtga ttgatgagct tgagattcaa
    10201 gcccaagaaa gccaattatt tggtgaaagg atagaagaag atgagaatca ataagtatat
    10261 tgcccacgca ggtgtggcca gtaggagaaa agcagaagag ctgattaagc aaggcttggt
    10321 gacggttaac ggccaagtgg tgcgtgaact agcaaccact atcaagtcag gcgacaaggt
    10381 cgaagttgaa ggtcaaccta tctacaacga agaaaaggtc tactatctgc ttaacaaacc
    10441 acgcggtgtc atttccagtg tgacagatga taagggtcgc aagacggttg tcgacctctt
    10501 gcccaatgtc aaagagcgta tttaccctgt gggtcgttta gactgggata catcaggagt
    10561 cttgattttg accaatgatg gggactttac agacgagatg attcaccctc gtaatgagat
    10621 tgacaaggtt tatgtcgcgc gtgttaaagg tgtggccaat aaggacaatc ttcgcccctt
    10681 gacccgtggt cttgagattg atggtaagaa aaccaagcca gctgtttatg aaattctcaa
    10741 agtggaccca gtcaaaaatc gctctgtggt gcagttgacc atccatgaag ggcgtaacca
    10801 tcaggttaaa aagatgtttg aagctgttgg tctccaagta gataagttgt ctcggactcg
    10861 tttcggacac ctagacttga caggactccg tccaggagaa tcccgtcgtc ttaataaaaa
    10921 agaaatcagc caactacaca ccatggctgt aactaagaaa taatgaaacg aattttaata
    10981 gcgcttgtgc gcttttacca acgttttatc tcaccagtct ttccaccctc ttgtcgcttt
    11041 gagctgactt gttccaacta catgattcag gctattgaaa aacatggttt taagggggta
    11101 ttgatgggct tggctcggat tttacgttgt catccctggt cgaaaacagg taaggacccc
    11161 gttccagacc acttttccct taaacgaaat caagaagggg aatgaggtgg ggtaaataga
    11221 tttcaaaatg ataaaaacgc atcctatcag gtttgagtga acttgatagg atgcgtttta
    11281 gaatgtcaaa attttatact cttcgaaaat ctcttcaaac cgcgtcagct ttcatctgca
    11341 acctcaaaac agtgttttga gcaacctgcg gctagtttcc tagtttgctc tttgattttc
    11401 attgagtatt aaattgagtt tgaagtggct tatttcaaag ctttttgtat gtcttcaatc
    11461 atgagttttg ttgattcaag tccgcctccg cttagatacc agaggtctgg tgttagttgg
    11521 ataatcttac catttttagc agcaggtgtt tcagcgataa gggcattttc taggacaccg
    11581 tcgttgctag agttgtcccc accgatggca agggtacggt tgatgacaaa gaggatgtca
    11641 gggttgattt ctttgacact ttcaaagctg acttcttgtc cgtggcgtga gtcttcaaat
    11701 tttgtatcag ttggtttgaa tttcaaggtt tggtacaaga aagagaaacg agatttggca
    11761 ccaaaggctg ccatttttcc ttcattaagg aggatcgcaa gggctttttt gtcagagctt
    11821 tcatttttag tagcgacttc ttggatgctc ttgtctagct tggccaattc ttccttggct
    11881 ttctgtgtac cagtttcgcc gaaggcactt gctaaggatt cgatattagc cttggtagaa
    11941 gtccagtagt cgtccttgct tgcttggaag agaacggttg gggcgatttc tttgaatttg
    12001 tctacgaatt tttgtgtacg tggtgaagcg ataatcaaat caggctcaag ggcggcgata
    12061 gcttctaaat caggttcttt catagaacca acatttttga cagttcccac taggtctttt
    12121 agataagtcg gaacagtttt tgtaggcatt ccgacgatat tttttgcaaa tcctaaagcg
    12181 cgaatagtat ccgcagcgcc gaggtcaaag gtcacaatct tttcaggaac tttggaaagt
    12241 ttgacctcgt ccagtgaact tttaatggtt acctctgttg gagcagagct actggtctct
    12301 gtctgactag tgcttgagtt tgtactacat gcaccaagta ggagcaagaa gctggccact
    12361 agggcagtga aataaagttt aagggatgtt ttcataattt ctccttttta aaatgtgata
    12421 acgatttagg gagtctctta atcttattga ctaagagact gaaggttctc taacttgagc
    12481 ttttatgtta ctagctatag atacagatct ttttgtcatt gatatcagct agcgtgatgg
    12541 gaatctcata aagttgactg agcaggtcag cctgcatgat ttgatcggtt cttcccttgc
    12601 taaagacctg gccgtccttg aaggcgacaa tttcatctgc atactgactg gccatgttga
    12661 tatcgtggag gacgatgata atggtcttgc cgagttcctc caccagtcgt cgaagaatct
    12721 gcatcatgct gacgctttgc ttgatatcga gattgttgag tggttcgtcc agcaagataa
    12781 agtccgtatc ctgggccagt accatagcga taaagacgcg ctggagttgc ccccctgaca
    12841 ggctattgat gtagcggtct tttaagttgg tcagttctaa atagttcaga gtttctcgga
    12901 ttttttccca gtcttctgat ctaagtcgac ctcggctgta gggaaaacgt ccaaaactga
    12961 ccagttcttc aacagtcaat ttggcttggt aattgatttt ctgttttagg atggttagtt
    13021 cttgggccag ttcttgcgaa ttccagctct cgatttcacg tcctttgata ctgagaactc
    13081 cctgatcttt cttggttagc ctgctcatga tggagaggag agtcgatttt ccagcaccat
    13141 ttggaccaat aaaggctgtc agtttttgag gactgacttc aagcgaaatg ccttgcaaaa
    13201 tatcctgttt ttgaatggat ttgtcaatgt tttccagttt cactgacgag acctcctata
    13261 tagtaagata aagaataaga agccacccac actctcaatg atcatactga tacgaatttc
    13321 cagtgcaaag actcgttcaa tcaaggcttg ccccaaggtt aagctaataa atccaaccag
    13381 aatggccact ataaagagta acttgtgctg atagtctttg acaatcaggt aggtgaggtt
    13441 ggccagtata aagccgaaga aggccatagg tcctaccaag gcagtggccg ttgaggtcaa
    13501 aagcacgatt ccccagagga gctctttctg ttctttttca acatcgagtc ccaatatctg
    13561 agccgtttct ctttgcaggt gcaagacatc tagaacgact gcttttcgaa agaaaaagat
    13621 tgtcaaagcg aggatgatca gagaaccgat ggctaggatg gaagtgttga gatgttgaaa
    13681 ggaggcaaaa agactatttt gcagtttatc gtattcgttt ggatccatta ggacttgaag
    13741 gaaggtgctg atatttcgaa agagacttct gagcgctaga cagatcagca ggatgaagac
    13801 caggtcttgc ttcatcagtg tcttcaagta accttgtaag gcgagaaaga agagggactg
    13861 gacaagaagt aagactagga attctaagat aggggatttg ccaagttgaa gaaacttgct
    13921 ttcaaaaacc agtagtaggg tttgtagtag gacgtagaag gattcaattc ccaaaatact
    13981 aggcgtcagg aagcgatttt ccgtcagggt ttgaaaacta atggtcgaaa tcccagtcgc
    14041 gatggctacc aagagataaa cgatgatctt ttgggaacgc aacttccaag caaaggctga
    14101 caagtgagtg atgggccaaa agtagagaag acaagctccg atggcaagaa taatgagaat
    14161 ccagaagagc ttggtatgtt tgcttttagt ctgcatcttt tcgtccccct ctccagagaa
    14221 gtaggataaa gacgagacta ccgatgattc ctagcaagag actgacagac aactcatagg
    14281 gcctaatcag aactcgggat aggatatcgc aagccagaac tagattggca ccaaccagtg
    14341 cgaccatgag tttggtttga cttagattat ctccatagca cttgcgaaca agattgggaa
    14401 cgataactcc gagaaatggt aggccaccca cggtaatcat ggtgacgctt gtcgttagcg
    14461 ccaccagaaa gagggccagt ttttcaagta gggagtagga aatccccaaa ctctcgctgg
    14521 tttctttccc tagattcatg atggtgaagg tttgggataa tttccaaacg gttatcagga
    14581 tgatgaggcc taagaagagc cactcatact gatgggtctg aatcatggag aaggagccct
    14641 gggtccaggc agtcatactc tgaaccagat tgaaacgata ggcgataact tctgtgactg
    14701 agccgataat cccgctatag atgatcccaa tcagaggcaa catccacctt tcctttacag
    14761 taaaaatggt cataaaggct aggaagaaga gggtgaatac gatggatgaa acaaaagcga
    14821 agagcatctt ttgggtcaga ctagccgatg gaaagacaaa aaggctcagc accattccca
    14881 gtttggcggc ttcagtcgtt ccaactgtac tcggtgcagc aaactgattt tgggtaatag
    14941 tctgcatgag aaggcctgcc atactcatac tagaggcagt caggagaata ctgatagttc
    15001 ttgggagacg ggactcttga aagaggagcc aggtctgctg gtcgaaatca aatagctttc
    15061 cccatgaaaa atcactggtc ccaatgctaa tagagagaaa gactaggagt agaagtaagc
    15121 caattaaata ataagaaagt ttcatacccc gtcctttcat gtagatttgg tatcgaaaga
    15181 tatctgcgga tataaatgta acattatttt tctaatctgt caataaaatt tctgacaatt
    15241 taataaatac aacaaggaga gagcaacaag actttctcct ttgttatcct attctaaaat
    15301 gtttttacct taatctgata aaataatatc ttcgagggag tagctagccg tccaatcaag
    15361 atattgttta gcttttgaag catctgctag gacactggct gggtcactag cacgtcgagc
    15421 aacaatctcg tgtgggattt tttaatttag taattcttca gcagttttaa agatttcttt
    15481 gatagtatag ccttttttag ttcctaagtt aaagatttct ttgatagtat agcctttttt
    15541 agttcctaag ttaaagattt gagaagaact gtcttcttga aataggtagt tcattccttt
    15601 aacatgagcc tatgaaggtc caagacataa atgtaatctc gaatacatga accgtcatgt
    15661 gtatcgtagt catctccaaa tatttttaag ctatcatttt gtcccaatgc ggtcttgttg
    15721 atatttggaa tgatgtgagt tggatttttc acacgcagac cgtttgaagc atccatttca
    15781 gccccagcaa cattaaagta acggaaaata acatatttcc agtcgtagcg attggccatc
    15841 cagtaaatca ttcgttcgcc catcagtttt gtctctgcat aagggttgac agggtcgagc
    15901 agggtatctt cagtcaccgg cttgtcaata cagttatttc catagagaga agcagtcgaa
    15961 gagaacatga ttttttgaat gccaacttca gataagactt tgagaacttg gttcctacca
    16021 gcaacgttgg cagtgaagta tttacttgga ttttcaatac tttcgctcac aacaatttca
    16081 cctgcacaat ggaggacagc atcaatctga ttttcttcta agtaagcctt taaggcgcta
    16141 gcatcataaa catccagttc tttaaagcta gcacgactat ccacagccgc tcgatttcct
    16201 gtggagagat tatcgaggac atgcacctta tagccagcat ttaaaagagc tttaacggta
    16261 tgggagccaa tgtagctagt cccacctgta acaagaattg ttttggtcat gattttttcc
    16321 ttttctagtt ttgtactata gtaaattgaa ataagatatg aacaactcta tt
//
LOCUS       DAVHIU010000036        16258 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVHIU010000036 DAVHIU010000000
VERSION     DAVHIU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.038"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            198..395
                     /locus_tag="VVN06_000997"
     CDS             198..395
                     /locus_tag="VVN06_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU5562567.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            463..1128
                     /locus_tag="VVN06_000998"
     CDS             463..1128
                     /locus_tag="VVN06_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562568.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFVVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            1200..2426
                     /locus_tag="VVN06_000999"
     CDS             1200..2426
                     /locus_tag="VVN06_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5562569.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(2822..2899)
                     /locus_tag="VVN06_001000"
                     /pseudo
     CDS             complement(2822..2899)
                     /locus_tag="VVN06_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2970..3626
                     /locus_tag="VVN06_001001"
     CDS             2970..3626
                     /locus_tag="VVN06_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU5562570.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            3616..3939
                     /locus_tag="VVN06_001002"
     CDS             3616..3939
                     /locus_tag="VVN06_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5562571.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            4043..4873
                     /locus_tag="VVN06_001003"
     CDS             4043..4873
                     /locus_tag="VVN06_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5562572.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            5027..5881
                     /locus_tag="VVN06_001004"
     CDS             5027..5881
                     /locus_tag="VVN06_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5562573.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            5981..7354
                     /locus_tag="VVN06_001005"
     CDS             5981..7354
                     /locus_tag="VVN06_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5562574.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            7347..8408
                     /locus_tag="VVN06_001006"
     CDS             7347..8408
                     /locus_tag="VVN06_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU5562575.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            8410..9102
                     /locus_tag="VVN06_001007"
     CDS             8410..9102
                     /locus_tag="VVN06_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562576.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(9132..9701)
                     /locus_tag="VVN06_001008"
     CDS             complement(9132..9701)
                     /locus_tag="VVN06_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU5562577.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            9836..10450
                     /locus_tag="VVN06_001009"
     CDS             9836..10450
                     /locus_tag="VVN06_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU5562578.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            10452..12098
                     /locus_tag="VVN06_001010"
     CDS             10452..12098
                     /locus_tag="VVN06_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5562579.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLNPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            12110..13396
                     /locus_tag="VVN06_001011"
     CDS             12110..13396
                     /locus_tag="VVN06_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5562580.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATVSHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAEFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKDLFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            13457..13990
                     /locus_tag="VVN06_001012"
     CDS             13457..13990
                     /locus_tag="VVN06_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562581.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            14068..14538
                     /gene="nrdI"
                     /locus_tag="VVN06_001013"
     CDS             14068..14538
                     /gene="nrdI"
                     /locus_tag="VVN06_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU5562582.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(14831..16093)
                     /locus_tag="VVN06_001014"
     CDS             complement(14831..16093)
                     /locus_tag="VVN06_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5562583.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4694 a         3025 c         3604 g         4935 t
ORIGIN      
        1 aataacttta ttataccagg gaaataatca aatctatcaa aatcgcaaat aagaaatttc
       61 tataagaaaa aatatcaaat atgcgatttt ttaaaataag ccaattttcg tgttatactg
      121 tacttgtaaa gcacttgaag caaatcctag gtcgcagaag tggtttacaa atgaagataa
      181 ttgaaggagt gtaaaagatg ttaaatttac aatttgcaga aacaatggaa ttgacagaag
      241 ctgagttgga gacagtttat ggaggggaat ttgggaataa tgctgttatc ccagccggtg
      301 cttggggagg tttaggaacg tcttggtcaa tcactaattt ctggaagaaa tattttaacc
      361 atgattcttc cactgttaat cgtcgccatt attgatattc agagtccttg cctttttctt
      421 gggtagggac tgtctttatt tcatagtata ggagaaaata gaatgaaaaa gataatctca
      481 caccgctact ttattatagt ttttctacta gttattgctg accagaagtt cagtgttcta
      541 gttttacgca gcgaccttgt tactgggcta tctgactttg cctattatct gtcggatatg
      601 atgttgaatt ttcttgtggt tttatttgct cttattgcta tgatttggtc ggggaaatgg
      661 cagaaaatca atagtagaaa gtttaaagga tcctatcttt tctattcctt cctagctctg
      721 cttgcttttg ttgtttggaa tttcgttacc ttttttcttt tcccacctac tcgaaatgaa
      781 atttcttatc aacatgctgc tccgactttt acaggggcta cagcattttt gatgtatttt
      841 ttctatcctg tgattgcagg tcccattttt gaagagataa tccatcgggg attggtgatg
      901 acagctctgg aaaaaggaaa gaaatggggg ctagatgtac ttggttctgc tattttattt
      961 ggggtctcgc acattagtaa tcacggttgg gtcttgacag actttgtctt ctatatgggt
     1021 ggcggactta tatttgtagt cttatttaga atgacaaagt ccatttattg gcctattggg
     1081 ctgcatatag tctacaacgg cattggtcag cttttgatgt tactgtaatt ttggttgtta
     1141 gtagtatcgc ggtctattct tggggaaaga ttcccatttg aatttaaaaa ggagttgtaa
     1201 tgaaagtatt tcttcaaaat agagatttta ggcaattaac tatcaaccag tggatttcaa
     1261 cgcttgggga tacgattttt tatctggcct tcctgaatta tgtggcagat gcgtcttttg
     1321 cccctttggc gattttactt atcacgattt cagaaaccct tccccaagtt ctacaaatct
     1381 ttctgggagt tttggcggat tttcaacatc atcgtgtctt gaaatacaca gtcattagtt
     1441 ttacaaaatt tttgctttac tctatagtgt ccctatcgct ttcagggcag tctttttcct
     1501 tgttgctagt aacctttatt tgtctgatta acctcttgtc tgacacattg agttactttt
     1561 caggagccat gctcactccg attttcatta gaattattgg gcaagaccat ctggcagaag
     1621 ctattggatt taaacagtca actgttagtt tagtgaaaac aatcagtaat attctaggag
     1681 gagtcttgct agggattctc tccatccagt tcatttcctt gctgaacgct cttacctttt
     1741 taatcgcatt tttaggtatc ctcttcataa aaactgacct cttgaaagta gaaaaaacga
     1801 ttaactatca agaaggactc tctgtaaaat ccttttgcca gcatttgctc caatcatcga
     1861 aattgatatg gaatatgaat aaggtgctct tggttttgtt tattatctct actagtcaag
     1921 cagtgataaa tgttacagtt cctgtctcta ctctattttt gaggaatcaa ccctttttga
     1981 atttacaaac aggtcaatct cttgccttgc tatccacttt tgaactgtca gccctgattg
     2041 tcggaagcct tgtaagtggc tatctgcaac ataccatttc cataaaaaca gctctctatg
     2101 cctcacttgt catccagttg cttcttctag taggatttgc cacagttcgt tttgactgga
     2161 ttctcatctt tagtaccttg gatgcctttt tcgcaggtgt tctctctcct agattgcaag
     2221 aactcgtctt taaacaaata cctgaggagt caatgggagc agttcaatcc tctatcggtg
     2281 ccattacggt tgttttacct agcttattta caatagcttt ggtaaccatt gctactagct
     2341 ttggaactct ggcagttagc tttgttttat tgctatttct tctagttgcc tttgttatgc
     2401 ttctgaatat tcgtgaaagt atttagtgga gaatttctat aatatatgtt tgagactgga
     2461 ttgccagtct ttttatgatg gtcaaaatca acagactttt ttctcttttc tgaatgtgtt
     2521 ataatagact gaatctaaaa tagtacgaaa caattgctaa aacatttata gaaattaatt
     2581 ttactttccc aatcgatttg ttctcatctt atttcaatct gctatataat agtccatgct
     2641 gtggctggat tcttttcagc ctacttatac tcttcgaaac tctcttcaaa ccacgtcagc
     2701 ttcactttgc cgtatatatg ttactgactt cgtcagttct atccacaacc tcaaaacggt
     2761 gttttgagct gacttcgtca gttctatcca caacctcaaa acggtgtttt gagctgactt
     2821 cgtcagttct atccacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag
     2881 tttgctcttt gattttcatt gagtattatt aagacaatat gaggagaaag atgaaagaaa
     2941 aaggatttta ggagggggcg caggcagcca tgccaacggc tcttggctat gtcagtatcg
     3001 gcctggcctg tgggactatc ggtgcgccct atgtgacacc tgttgagatg ggcttgatga
     3061 gtctctttgt ttatgctggg agtgcccagt ttgccatgtt ggcactgatt gtggttcaag
     3121 ctcctgtggc agctattgct atgacggttt ttctaatcaa cttgcgtctc tttttgttga
     3181 gtttacacgc atcgacttat ttccgtcata ccagtctttg gtacaatatc ggtatgtcta
     3241 gtatcttgac agatgagacc tatggcgttt tgatgggtga attggcccat acagacaagg
     3301 taaatcctat gtggatgcac ggaaacaatc ttaacagcta tgtggcttgg tttgtgggga
     3361 cagtagtcgg aacggctctg ggtggcctgc taccaaatcc agaaatcttt ggcttggatt
     3421 ttgccctggt tgggatgttt attggtattt ttgcttcgca atttcagatt atgcaaagac
     3481 ggattcctgt ccgcaatctg ctcattatcc tagcagttgt tgcggtgtcc ttctttttgc
     3541 tcttgacagt ggtgtctcag tcactagctg ttctgtttgc gacgctactt ggttgtagca
     3601 tgggggtggt tttagatggt cagtaagtat cttttattag cagttatttt ctctggcttg
     3661 gtgacttgga ttctccgtat gattcccttc atcttagtca agtataaggg cttgcctgca
     3721 atcgttgagc gttttttgaa gttcttgccc gtttccatta tctttgcctt gattctttca
     3781 agcgtagtga caggtaaggt tgggagcctt cctcaaatta aatggctaga cttcttagcc
     3841 gtctttccaa cagcttgggt agcctttcgc taccgcaatc tagtcggaac agttctcttt
     3901 ggagtggtct tgattgccat cttgcgtttg gtcttttaaa ttacccataa aaaaaactta
     3961 tcacagagat agatatcata taatggcgta aatgctcttt ttctgttaag attataaggt
     4021 attctatttt ggaggaaatg acatgaaaaa aatcgttaaa tactcatctc ttgctgccct
     4081 aggacttgtt gctgcaggtg tgcttgcggc ttgctcaggg ggtgctaaga aagaaggaga
     4141 agcagctagc aagaaagaaa tcatcgttgc aaccaatgga tcaccaaggc catttatcta
     4201 tgaagaaaat ggcgaattga ctggttacga gattgaagtc gttcgcgcta tctttaaaga
     4261 ttctgacaaa tatgatgtca agtttgaaaa gacagaatgg tcaggtgtct ttgctggtct
     4321 tgacgctgac cgttacaata tggctgtcaa caatcttagc tacactaaag aacgtgcgga
     4381 gaaatacctc tatgccgcac caattgccca aaatcctaat gtccttgtcg tgaagaaaga
     4441 tgactctagt atcaagtctc tcgatgatat cggtggaaaa tcgacggaag tcgttcaagc
     4501 cactacatca gctaagcagt tagaagcata caatgctgaa cacacggaca acccaactat
     4561 ccttaactat actaaggcag acttccaaca aatcatggta cgtttgagcg atggacaatt
     4621 tgactacaag atttttgata aaatcggtgt tgaaacagtg atcaagaacc aaggtttgga
     4681 caacttgaaa gttatcgaac ttccaagcga ccaacaaccg tacgtttacc cacttcttgc
     4741 tcaaggtcaa gatgagttga aatcatttgt agacaaacgc atcaaagaac tttataaaga
     4801 tggaactctt gaaaaattgt ctaaacaatt cttcggagat acttatctac cagcagaggc
     4861 tgatattaaa taatttcttg aaatcatcca ttctgagtaa gatgggtgat ttctcagttt
     4921 ttagggatat agttttaaac tatatatctg attatctaat aacaatttgt gaaatcaaac
     4981 aaattgtgag atactagtac ggtattattt ttaaggagaa agaatcatga aaatcaaaaa
     5041 atggcttggc ctagtagccc ttgctacagt cgcaggtttg gctcttgcag cttgcggaaa
     5101 ctcagaaaag aaagcagaca atgcaacaac tatcaaaatc gcaactgtta accgtagcgg
     5161 ttctgaagaa aaacgttggg acaaaatcca agaattggtt aaaaaagacg gaattacctt
     5221 ggaatttaca gagttcacag actactcaca accaaacaaa gcaactgctg atggcgaagt
     5281 agatttgaac gctttccaac actataactt cttgaacaac tggaacaaag aaaacggaaa
     5341 agaccttgta gcgattgcag atacttacat ctctccaatc cgcctttact caggtttgaa
     5401 tggaagtgcc aacaagtaca ctaaagtaga agacatccca gcaaacggag aaatcgctgt
     5461 accgaatgac gctacaaacg aaagccgtgc gctttacttg cttcaatcag ctggcttgat
     5521 taaattggat gtttctggaa ctgctcttgc aacagttgcc aacatcaaag aaaatccaaa
     5581 gaacttgaaa atcactgaat tggacgctag ccaaacagct cgttcattgt catcagttga
     5641 cgctgccgtt gtaaacaata ccttcgttac agaagcaaaa ttggactaca agaaagcact
     5701 tttcaaagaa caagctgatg aaaactcaaa acaatggtac aacatcattg ttgcgaaaaa
     5761 agattgggaa acatcaccta aggctgatgc tatcaagaaa gtaatcgcag cttaccacac
     5821 agatgacgtg aaaaaagtta tcgaagaatc atcagacggt ttggatcaac cagtttggta
     5881 ataagaaaca gggaggtggg agagagaatt ccacctcttg cttttgtata gagcacggag
     5941 tgtaaagaag actattcgtt catagaaagg tagagagaat atggtttttc ctagcgaaca
     6001 agaacagatt gaaaaatttg aaaaggatca tgtagcccag cattattttg aggttttgcg
     6061 taccttgatt tctaagaaat cagtctttgc ccagcaggtt ggactcaagg aagtcgcaaa
     6121 ttatctgggt gagattttca agcgtgttgg agctgaagtg gagattgatg agagctatac
     6181 agcgcccttt gtcatggcac atttcaagag ttcgcgtcca gatgccaaga ccttgatttt
     6241 ctataaccac tatgacactg tgccagcgga tggggatcag gtctggacag aggatccttt
     6301 tacgctttcg gtccgcaatg gcttcatgta tgggcgtgga gttgatgacg acaagggtca
     6361 tatcacagct cgcttgagtg ctttgagaaa atatatgcag caccatgatg atttacctgt
     6421 caatatcagc tttatcatgg agggagcgga ggaatcggct tcaacagacc tagataagta
     6481 tttggaaaag catgcagaca aactccgtgg ggcggatttg ttggtctggg aacaagggac
     6541 caaaaatgcc ttggaacagc tggaaatttc tggtggcaat aaggggattg tgacctttga
     6601 tgccaaggta aaaagcgctg atgtggatat ccactcgagt tatggtggtg ttgtggaatc
     6661 agctccttgg tatctcctcc aagccttaca gtctcttcgt gctgcggatg gccgtatctt
     6721 ggttgaaggc ttgtacgaag aagtacaaga gcccaatgaa cgagaaatgg ccttgctaga
     6781 aacttatggt caacgaaacc cagaggaagt tagtcggatt tatggattgg agttgcctct
     6841 cttacaggag gagcggatgg cctttctaaa acgtttcttt ttcgatccag cgcttaatat
     6901 cgaaggaatc cagtctggtt atcaaggtca gggtgttaag actattttac ctgcagaagc
     6961 cagtgccaag ctagaggttc gtctggttcc gggcctagaa ccgcatgatg ttctggaaaa
     7021 aattcggaaa cagctagaca aaaatggctt tgataaggta gaattatact ataccttggg
     7081 agagatgagc tatcgaagcg atatgagcgc accagccatt ctcaatgtga tcgagttggc
     7141 caagaaattc tatccacagg gcgtttcagt cttgccgacg acagcgggga caggacctat
     7201 gcatacggtc tttgatgccc tagaggtacc aatggttgca ttcggtctag gaaatgccaa
     7261 tagccgagac cacggtggag atgaaaatgt gcgaatcgct gattattaca cccatatcga
     7321 attagtagag gagctgatta gaagctatga gtagagatat tatcaagtta gatcagatcg
     7381 atgtgacttt tcaccaaaag aagagaacca tcacagcggt taaggatgtg accattcaca
     7441 tccaagaagg ggatatctac ggaatcgttg gatattctgg agcaggaaaa tcaacccttg
     7501 tacgggtgat taatcttttg caaaaaccat ctgcagggaa aattaccatt gacgacgatg
     7561 tgatttttga cggcaaggtg accttgacgg cagagcagtt gcgtcgtaaa cgtcaagata
     7621 tcgggatgat tttccagcat tttaacctga tgagccaaaa gacagcagag gagaatgtag
     7681 cctttgccct taaacactct ggactcagca aggaagaaaa gaaggctaaa gtagctaagt
     7741 tgttggactt ggttggtttg gcagatcgtg ctgaaaacta cccttcacaa ctatctggag
     7801 ggcaaaaaca gcgtgtggca attgcgcgtg ccttggccaa tgatccaaaa atcttgattt
     7861 cagacgagtc aacttctgcc cttgacccta agacaaccaa gcagattttg gccttgttgc
     7921 aagatttgaa ccaaaaatta ggattgacag ttgtcttgat tacgcatgaa atgcagattg
     7981 tcaaagacat tgccaaccgt gtggcggtta tgcaggatgg gcatttgatt gaagagggaa
     8041 gtgtccttga aatcttctca aaccctaaac aacctttgac tcaagacttt atctcaacag
     8101 ccacaggtat tgacgaagcc atggtcaaaa tcgagaagca agaaatcgtg gaacacttgt
     8161 ctgaaaacag tctcttggtg caactcaagt acgctggagc ttcaacagac gagccacttt
     8221 tgaatgaatt gtacaagcat taccaagtaa tggctaatat tctctatggg aatatcgaaa
     8281 ttctcgatgg tactcctgtt ggagaattgg tggtggtctt gtcaggtgaa aaagcagcgt
     8341 tggcaggtgc ccaagaagcc attcgtcaag caggtgtaca actaaaagta ttgaagggag
     8401 tacagtaaga tggaatcatt gattcaaacc tatttaccaa atgtctataa aatgggttgg
     8461 gctggtcagg caggctgggg aacggctatc tacttaactc tttatatgac agttctttcc
     8521 ttcattatcg gaggcttctt ggggctagtg gcaggtctct ttctcgtctt gacagcgcca
     8581 ggtggtgtct tggagaataa agtcgtattc tggattttag acaaaattac ctcaattttt
     8641 cgtgcggttc cctttatcat cctcttggca atcttgtcac cactttctca cttgattgtt
     8701 aaaacaagta tcgggccaaa tgcagccctt gtcccacttt cttttgcagt ctttgccttc
     8761 tttgcccgtc aggtgcaggt tgtcttggct gaattggatg gcggtgtcat tgaggcggct
     8821 caagcgagcg gagcgacttt ctgggacatc gtgggtgttt acctatcaga aggtcttcca
     8881 gatttgatcc gtgtgacgac tgtgaccttg atttcccttg ttggggaaac agctatggcc
     8941 ggtgcggttg gagctggtgg tatcggtaac gtagccatcg cttatggatt taaccgctac
     9001 aatcacgatg tgaccatctt ggcaaccatt attatcattt tgattatctt tgcaatccaa
     9061 ttcttgggag atttcttgac taagaaattg agccataaat aaaaaagagc cgcgtggctc
     9121 tttttaactg atcagatttt ctgtgcaaat tttttactca aggcttgtcc aatcaaggca
     9181 cccactaggg ctccgatgac aatacttgcg ataaatagaa ggacagttcc agggtttgga
     9241 gcgaccatga tacggtcgat atattcttgg gattttcctc ttgccagaag agtagccata
     9301 taggctttgg gcgcaatcca cataagcaag attggtcctg ttgtactaaa ggcgaaaata
     9361 atgaaagaaa ggaagttctt tgttttgtcc ttgtattttc ctaaatgagc tacttcatct
     9421 gctaggaggc cacagataat tccaggaagg aaggaaccag caccgtgttt agttcccaag
     9481 aaaaagaggg caatgacaag gccgatagtg gtaatggctc caaagcgcgg aacttttgcg
     9541 actaggatca tatagacgct accgccgaca agggcagtaa aggcaggcgc ataaaacatg
     9601 tttccagttt ggtcaaagag attgcccaaa aggacaccaa tccccatgca gaggaagtaa
     9661 agaactgcaa aaagcagtgt agttaagata gatttcttca tgaattgtct cctgataatt
     9721 ttttcacaat tctcattgta ccacggtttg gtgggattgt aaatggatac tagaagtttt
     9781 tgaaaacgct tgaaatatga tataatggtt tcatagttat ttttagggga atatcatggg
     9841 tagattttta gactttgtct ttaatcgttt ctttttaggg atgattgcga cagccttctt
     9901 ttggctatta actttagcag gagggattat ccttggtcta gcgccggcta gtgccacctt
     9961 gatgagctta tatgcagaac atggttatag ctttcgggaa tacagtttga aggaggcttg
    10021 gtctctttac aagcaaaatt ttgtctcaag caacctgatt ttctatagct ttttaggtgt
    10081 gggtctagtt ttgacctatg gtttgtatct cttggtgcaa ttgcctcatc agaccattgt
    10141 tcatttgatt gcgacccttt tgaatgtcct agtagttgcc ctgatctttt tggcttatac
    10201 agtatcttta aaattacaag tttattttgc cttgtcctat cgaaatagtc tcaaattatc
    10261 cttgattggc atctttatga gtctagcagc tgtggccaag attctccttg ggactgtgct
    10321 acttgtagca attggttact atatgcctgc cctgctattt tttgtaggaa ttgggatgtg
    10381 gcatttcttt atcagtgata tgttggaacc tgtctatgaa atcatccatg aaaaattggc
    10441 gacaaaatag aatgaagcag ttttggctac atacgcttct aagaacctat agttcagtga
    10501 tgatcattat cattgcgagt tttgcaatct tactctctta cgctgactgg gattcacgtg
    10561 aaaaggaagc ccagagagta gcccagcgtg taactgctag aacagtgagt gaaattgaat
    10621 attaccatag agagtcaacc cagatagctc aggctttagt tgaaaaccaa gctcgtattg
    10681 agggaatcta taaatacttt agtcttagca tgccagacta tttttactgg caattagagc
    10741 ggaaagcttc gccttatata tcagtctctc tgtatgaaaa tgttgatgac ctctatgttc
    10801 gaaatgattt tgtaactgga gtggccattg cttttcaaga ttacaaggaa gtctatgttt
    10861 ctactaaaga caaacgtagt ggagaaaaaa tcagggctga ggatttcaaa ccagcaggaa
    10921 atagttttgc cattccggtg tcagatccag tgtcagatca agatttagga gtgatttaca
    10981 tctccttgaa tcctgctgtt ttataccatg ccattgataa tactagaggt catactccga
    11041 tggcagtaac agtgacctca ccttttgata cggagatttt tcatattggt gagacagttg
    11101 atagggagag tgaaaattgg ctagttggct taacttctca tggatatcag gttcaggtgg
    11161 cagttcctaa aaactttgtt ttacaaggaa cagtgactag ctctgctttg attgtgggtt
    11221 tgagccttct ctttattgtc attctttatc tgactttgag gcagactttt gctaattacc
    11281 aaaagcaggt agtggattta gtggaatcca ttcaagtcat tgctcaaggc gaagaggggc
    11341 gtcggattga catttccgaa aaagatcagg aattactcct aatcgcggag acgaccaatg
    11401 atatgttgga tcgattggaa aagaatatcc atgatattta ccagttagag cttagtcaaa
    11461 aagatgccaa tatgcgagcc ttgcaggcgc aaatcaatcc tcattttatg tataatacgc
    11521 tggagttctt gcgcatgtat gcagttatgc agagtcaaga tgagttggca gatatcattt
    11581 atgaattcag tagtctcttg cgtaacaata tttccgacga aagagagacc ctcctcaaac
    11641 aggaattaga attttgccgt aaatacagct atctctgcat ggttcgctat cccaagtcca
    11701 ttgcctatgg tttcaagata gatccagagt tagagaatat gaagattccc aagtttacct
    11761 tgcaaccgct ggtagaaaac tatttcgcgc atggtgttga ccacaggcgg acagataatg
    11821 tgattagcat caaggctctt aaacaggatg gttttgtgga aattttggtg gtcgataatg
    11881 gtagaggaat gtcggctgaa aagttggcaa atatccgaga aaaattaagt cagagatatt
    11941 ttgaacacca agccagctac agtgatcaaa ggcagtctat cgggattgtc aatgtacacg
    12001 agcgttttgt gctctatttt ggagaccgct atgccattac tatagagtct gcagagcaag
    12061 ccggtgttca gtatcgtatt acaattcaag atgagtagaa agggagaaaa tgtataaagt
    12121 attattagta gatgatgagt acatggtgac agaaggtctg aagcgtttga ttccctttga
    12181 taagtgggat atggaggtcg tcgcaacagt cagtcatgcc gatgaagctc tagaatatgt
    12241 tcaggaaaat cctgtcgatg tcatcatttc cgatgtcaat atgccagaca aaacagggct
    12301 tgatatgatt cgggagatga aagagatctt accagatgct gcctatatcc tgctctcagg
    12361 ttatcaggag tttgattatg taaaaagagc aatgaacctt agtgtggtgg actatttggt
    12421 caaacctgtt gataaggtag agctgggaaa tctgctggag aagattgcag gtcagctcgg
    12481 cgagagaggg aagaaaagtc agactcttag tcaagaatta gacgaggctg aatttgttag
    12541 ttatttaggg gataaggaga attggtggat aggtctatcc aaggaaaaac aaggttcctt
    12601 caccattccc tactatgtct tgggccagga ctggcagatt ttcatttctg accaacccct
    12661 agatggttta gtcgttacac cttttgaagc tccttatcaa gaacactttg aacgctggaa
    12721 gctgaatgct gagaaaaccc tcttttacgg ttctgtaaat ttgcagcagt ctgagagtct
    12781 ctttgcctat tacgaaccga tttatagggt tatcattcag ggaaatctca atcaaatcgt
    12841 agaagagtta aatctcttgg agaaggtagt tcttgaaaat acgccgcgtg ttccgattac
    12901 taaacagctt tttattcagt ttgtcatgga tgttttccat ttatttgaac atctcaaagc
    12961 tgatgacctg acagacatcg tcaaaaccat tcatgctatt caatccttcg atgaattggt
    13021 tccttatatc aaggaaactc tgaccagctt tttcggtcaa taccgtatga atgaaaatgt
    13081 ggtcagtgtg ctggaagtca ttggtcgtga ttaccaaaaa gagctttccc tcaaggatat
    13141 cagtaaggat ctctttatca atcctgtcta tctagggcag ttgattaagc gtgaaaccga
    13201 ttcgactttt gcagagttac taaacaaaca acgtattaag gctgcccaac aacttttgct
    13261 ttcaactagt gacagcatcg aagatatttg ttatgctgtt ggttacagta accttggata
    13321 tttctataaa gttttccgaa aattgtgcgg aaaatcgcca aaagcctacc gaaaacaggt
    13381 agaaactata ctataagatt tgtattcctt tacaaaatgt gctataatat aaatattatt
    13441 atcaggaggt tctatcatga aaaagaaacc aatttatcta tgggtcttgc taatcttgtc
    13501 tgcccttatt tcagttccgt ctctgtttgg aattgtgagt cccttgccta gtaaagaagc
    13561 ccttcgtact gctcagaaac aagttgcagg ggtcaatgct cagcaattag aagaccagct
    13621 taattatacc tatagagtag cagaagcatc tcattctatt tttaatgttg ctttgattgt
    13681 gctatctact attttagtgg tcgtagcgat tgtcttcctt gttcgtaaaa atttgcaata
    13741 tgcaaactat acttacgttg gctatgtttt gttagctatt attggttcga tttatggcta
    13801 tgttggttta caagacgctg tgcagttggt tcaagatgag agcatgcgtt tgacagtgag
    13861 tattggttca aaagctgtta gcattttcta tatcgtcatc aatgttctct tcctagccct
    13921 tgtcttctat aagatgtggc gacaacagaa agccttggca gaagaagagg aaacagaaga
    13981 gcttacctaa gtattgacaa aaaagaacta tcaagataca atcttggtag ttgttttttg
    14041 attatagata aaatcatgga ggtacatatg aagacaattt ctttagttta tattagtttg
    14101 agtggcaaca ctgaaagttt tgtgacgcgc ttgaaagact atctcttgtc ccagtacaaa
    14161 gggattgagg ttcaaaagat tcatatcaag gatttggtca aggaaggcaa aaatttctat
    14221 gaaatggacc atccctatgt cgcttttttg ccgacctatc tcgaaggtgg gaatggcgtg
    14281 gataacggag atgttgagat tttgacgaca ccggtgggag attttattgc ctatggtaac
    14341 aatgctagta agtgttttgg tgtggttggg tcaggaaatc gtaactttaa taaccaatac
    14401 tgcctaacag ccaagcaata cagtcaacgc tttggcttcc ctgtattggc tgactttgaa
    14461 atgcgaggca tgctggaaga tatcaaacat gtcgcagcta ttatcgcaga tttgtatgag
    14521 ttggaaaagg agaattaata ctcaatgaaa atcaaagagc aaactaggaa gctagtcgca
    14581 ggttgctcaa agcacagctt tgaggttgca gatagagctg acgtggtttg aagagattct
    14641 cgaatagtat aaagttctta taagatgtgg ttgaagttgt tcaactgttt tttgagtata
    14701 aacagtcttt aagaacgtaa aaaagcataa gttcaggtct tcaaaacctt gttcttatgc
    14761 tttttatcat agaaatattt gctgaatttt ctcttgaaat gaaaccttat cttcatctcg
    14821 cgcttgtccc ttaccctgct tttccgattg cgagtgcgta gataaagaag atggcgaagc
    14881 caaagaggaa aatcaatccc gcaatggcaa ggtgtttgag ccaagaagga agatttttgt
    14941 tttcacgtgt taccaaggcg taattcaaaa gagcaaagaa aggtgttgtc aggaaagagc
    15001 caatcatggc aaagcggagc atggttgaaa cctgaccagc gaagaacttg ataatgacga
    15061 taccgatgat agcagtgatg gtcatccaga tgttcaaaga tttacgattg tcctcttttt
    15121 gactgattag cagtcggaga gattcctgat taacgcgaga atagccatcg ataacagtta
    15181 taactgttcc aaagatacag aggaaggcaa taaaggtaat caagtaacgg gaccattcgc
    15241 caagaacaga ggcatacatg cccacgaatt gagagatgta tttggctgaa gcagcttcaa
    15301 ccgcctgccc tgtaggatac tgaatcagtg ctcccagtgc cacaaagaag acggctagga
    15361 tagctgttcc aatataacca gtgttaaagt caaacagagc gtcttctgtg ttaaagttga
    15421 cggtctttct cttttcagct gaccaaagtg aattgatggc tgaaatttca ataggagccg
    15481 gcatccatcc taggagggag acgatgaagg gcagagctgc catttgccaa ggtgtcttct
    15541 cgacaaaatc agaactgtat tctggatgct tgaccgccgc aatgataact gcaagaacag
    15601 tcgcaatggt taaggcagac ataatccatt tgaccatgcc gtctaaaagt ttgtagcctc
    15661 caaagagtag catagcccaa atgattgcaa cgagaatgag ggaccactga gtaatgctaa
    15721 gtccaatcat tgggaaggca ctggcgatga tagctgagca cagaatggca acaccagccg
    15781 tgttgaccat agccgaaaag acattgagaa taaagaaaat ccagagatag agttttcctt
    15841 tttcggcata accttcaacc aaagtctttc cagtatcagc tgtgtattca gcaccaaaac
    15901 ggaaaaatgg atatttaaag acattggcta agatgaccaa gagaagtaga gaccaaccgt
    15961 aagaaccgcc agcttgagtt gaggatacaa tgtgggaacc tccaacagcg gcagttgcca
    16021 ttaggattcc aggtcccatt gcttttagct tgcttgccca ggttgattga tttaaggaaa
    16081 tagcttgcga catgataaat actccttttt gaattttcag aataatctgt atatgcaatc
    16141 tatattctac aaaaaatttt ggaaaaatgc aagaatattt tgaaaaaaga tagaaaattt
    16201 acaaagaaaa tctgaaataa ctgttatgat atagcagatt gaaactagaa tagtacac
//
LOCUS       DAVHIU010000037        15988 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVHIU010000037 DAVHIU010000000
VERSION     DAVHIU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.039"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            17..2182
                     /locus_tag="VVN06_001015"
     CDS             17..2182
                     /locus_tag="VVN06_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU5562584.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQTGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2182..2922
                     /locus_tag="VVN06_001016"
     CDS             2182..2922
                     /locus_tag="VVN06_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5562585.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3071..4558
                     /locus_tag="VVN06_001017"
     CDS             3071..4558
                     /locus_tag="VVN06_001017"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5562586.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(4602..5891)
                     /gene="ftsY"
                     /locus_tag="VVN06_001018"
     CDS             complement(4602..5891)
                     /gene="ftsY"
                     /locus_tag="VVN06_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5562587.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEADEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRTGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5895..6713)
                     /locus_tag="VVN06_001019"
     CDS             complement(5895..6713)
                     /locus_tag="VVN06_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5562588.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6713..7507)
                     /locus_tag="VVN06_001020"
     CDS             complement(6713..7507)
                     /locus_tag="VVN06_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5562589.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSHDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7504..11043)
                     /gene="smc"
                     /locus_tag="VVN06_001021"
     CDS             complement(7504..11043)
                     /gene="smc"
                     /locus_tag="VVN06_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU5562590.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQYIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDRYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11034..11732)
                     /locus_tag="VVN06_001022"
     CDS             complement(11034..11732)
                     /locus_tag="VVN06_001022"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5562591.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(11915..11986)
                     /locus_tag="VVN06_001023"
     tRNA            complement(11915..11986)
                     /locus_tag="VVN06_001023"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11951..11953),aa:Arg,seq:ccg)
     gene            complement(12050..13036)
                     /locus_tag="VVN06_001024"
     CDS             complement(12050..13036)
                     /locus_tag="VVN06_001024"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU5562592.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13211..14527)
                     /locus_tag="VVN06_001025"
     CDS             complement(13211..14527)
                     /locus_tag="VVN06_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU5562593.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFFVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKTEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14514..>15988)
                     /locus_tag="VVN06_001026"
     CDS             complement(14514..>15988)
                     /locus_tag="VVN06_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU5562594.1"
                     /translation="RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYL
                     QNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLK
                     SPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGD
                     GAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLS
                     VNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLK
                     DYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHET
                     DEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEE
                     AKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGS
                     YDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4587 a         3560 c         2889 g         4952 t
ORIGIN      
        1 gaaaaggaga ctaaaaatga agaaaaaatt tctagcattt ttgctaattt tattcccaat
       61 tttctcatta ggtattgcca aagcagaaac gattaagatt gtttctgata ccgcctatgc
      121 accttttgag tttaaagatt cagatcaaac ttataaagga attgatgttg acattattaa
      181 caaagtcgct gagattaaag gctggaacat tcagatgtcc tatcctggat ttgacgcagc
      241 agtcaatgcg gttcaagctg ggcaagccga cgctatcatg gcagggatga caaagactaa
      301 agaacgtgaa aaagtcttca ccatgtctga tacttactat gatacaaaag ttgtcattgc
      361 tactacaaag tcacacaaaa ttagcaagta cgaccaatta actggcaaaa ccgttggtgt
      421 taaaaacgga actgccgctc aacgtttcct tgaaacaatc aaagataaat acggctttac
      481 tattaaaaca tttgacactg gggatttaat gaacaacagc ttgagtgctg gtgccatcga
      541 tgccatgatg gatgacaaac ctgttatcga atatgccatt aaccaaggtc aagacctcca
      601 tattgaaatg gatggtgaag ctgtaggaag ttttgctttc ggtgtgaaaa aaggaagtaa
      661 atacgaacac ctggttactg aatttaacca agccttggct gaaatgaaaa aagatggtag
      721 tcttgataaa attatcaaga aatggactgc ttcatcatct tcagcagtgc caactacaac
      781 cactctcgca ggattaaaag ctattcctgt taaggctaaa tatatcattg ccagcgattc
      841 ttcttttgca ccttttgttt tccaaaattc aagcaaccaa tacactggta ttgatatgga
      901 attgattaag gcaatcgcta aagaccaagg ttttgaaatt gaaatcacca accctggttt
      961 tgatgctgct atcagtgctg tccaaactgg tcaagccgat ggtatcattg ctggtatgtc
     1021 tgtcacagat gctcgtaagg caacttttga cttctcagaa tcatactaca ctgctaatac
     1081 cattcttggt gtcaaagaat caagcaatat tgcttcttat gaagatctaa aaggaaagac
     1141 agtcggtgtt aaaaacggaa ctgcttctca aaccttccta acagaaaatc aaagcaaata
     1201 cggttacaaa atcaaaacct ttgctgatgg ttcttcaatg tatgacagtt taaacactgg
     1261 tgccattgat gccgttatgg atgatgaacc tgttctcaaa tattctatca gccaaggtca
     1321 aaaattgaaa actccaatct ctggaactcc aatcggtgaa acagcctttg ccgttaaaaa
     1381 aggagcaaat ccagaactga ttgaaatgtt caacaacgga cttgcaaacc ttaaagcaaa
     1441 cggtgaattc caaaagattc ttgacaaata cctagctagc gaatcttcaa ctgcttcaac
     1501 aagtactgtt gacgaaacaa cgctctgggg cttgcttcaa aacaactaca aacaactcct
     1561 tagcggtctt ggtatcactc ttgctctagc tcttatctca tttgctattg ccattgtcat
     1621 cggaattatc ttcggtatgt ttagcgttag cccatacaaa tctcttcgcg tcatctctga
     1681 gattttcgtt gacgttattc gtggtattcc attgatgatt cttgcagcct tcatcttctg
     1741 gggaattcca aacttcatcg agtctatcac aggccaacaa agcccaatta acgactttgt
     1801 agctggaaca attgccctct cactcaatgc ggctgcttat atcgctgaaa tcgttcgtgg
     1861 tggtattcag gccgttccag ttggccaaat ggaagccagc cgaagcttgg gtatctctta
     1921 tggaaaaacc atgcgtaaga ttatcttgcc acaagcaact aaattgatgt tgccaaactt
     1981 tgtcaaccaa ttcgttatcg ctcttaaaga tacaactatc gtatctgcta tcggtttggt
     2041 tgaactcttc caaactggta agattatcat tgctcgtaac taccaaagtt tcaagatgta
     2101 tgcaatcctt gctatcttct atcttgtaat tatcacactt ttgactagac tagcgaaacg
     2161 cttagaaaag aggattcgtt aatggcaaaa ttaaaaattg atgtaaatga tttacacaag
     2221 cactatggaa aaaatgaagt cctaaaagga attacgacta agttctatga aggagatgtt
     2281 gtttgtatca tcggtccttc aggttctggt aagtcaactt tcctccgtag cctcaatctt
     2341 ttagaagaag tcactagcgg tcacatcact gtgaacggct atgatttaac tgaaaaaaca
     2401 accaatgttg accacgtccg tgaaaatatc ggcatggtat tccaacactt caacctcttc
     2461 cctcatatgt ctgtattgga caacatcacc tttgctccta ttgagcacaa gttgatgact
     2521 aaggaagaag ctgaggaatt gggaatggag ttgcttgaaa aggttggact agcagataaa
     2581 gctaatgcca atccagatag cctatcaggt ggtcaaaaac aacgtgtggc catcgctcgt
     2641 ggcctagcaa tgaatccaga catcatgctc ttcgatgaac caacttctgc ccttgaccct
     2701 gagatggttg gagacgtact taacgttatg aaggaattgg ctgagcaagg catgaccatg
     2761 attatcgtaa cccatgagat gggatttgct cgtcaggttg ccaaccgcgt tatctttact
     2821 gcagatggcg agttccttga agacggaaca cctgaccaaa tctttgataa cccacaacac
     2881 cctcgtctga aagagttctt agataaggtc ttaaacgtct aaactcaaac tgtaaggatt
     2941 tccttgcagt ttttctacct cgtattggaa tttttgattt ttcggaaaat tatgttagaa
     3001 ttaagtttat gaaatgaggt ttcctcatac ctagcaagac taggaataaa aatagaaatt
     3061 aggtagctag atgtcatcta aggttattgt tacaattttc ggtgcgagtg gagacctggc
     3121 taaacgcaag ctctaccctt ccctttttag actatataaa tccggcaatc tttccaagca
     3181 ctttgccgtt attggaactg cccgtagacc ttggagtaag gaatattttg aatctgtagt
     3241 tgtcgagtcc atccttgatt tggcagatag taccgagcaa gcccaagaat ttgctagcca
     3301 cttctactat caaagccatg atgtcaatga ttcggaacat tatattgctt tgcgtcaatt
     3361 acaagctgag cttaatgaaa aataccaagc tgaacacaat aagctcttct tcttgtctat
     3421 ggcacctcag ttctttggaa ccattgccaa acacctcaaa tctgaaaaca ttgtcgatgg
     3481 caaaggtttt gagcgcttga tcgttgaaaa accatttggt acagattacg caactgcaag
     3541 caagttgaat gacgaactcc tagcaacatt tgacgaagaa caaattttcc gtatcgacca
     3601 ttatcttggt aaggaaatga tccaaagcat ctttgcagtt cgctttgcaa acttgatttt
     3661 tgaaaacgtt tggaacaagg attttatcga caatgttcaa attacctttg cggagcgctt
     3721 gggtgtagaa gaacgtggtg gctactatga ccaatccggt gccctccgtg acatggtcca
     3781 aaaccacact ctacaacttc tttcgctcct cgctatggac aaaccagcaa gcttcacaaa
     3841 agacgagatt cgtgctgaaa agattaaggt ctttaaaaac ctctatcatc caactgatga
     3901 agaactcaaa gaacacttta tccgtgggca ataccgctct ggtaagattg atggcatgaa
     3961 atacatctct tatcgtagcg aaccaaatgt gaatccagaa tcaacaactg aaacctttac
     4021 atctggtgcc ttctttgtag acagcgatcg attccgtggt gttcctttct ttttccgtac
     4081 aggtaaacga ctgactgaaa aaggaactca tgtcaacatc gtctttaaac aaatggattc
     4141 tatctttgga gaaccacttg ctccaaatat tttgaccatc tatattcaac caacagaagg
     4201 cttctctctt agcctaaatg ggaagcaagt aggagaagaa tttaacttgg ctcctaactc
     4261 acttgattat cgtacagacg cgactgcaac tggtgcttct ccagaaccat acgagaaatt
     4321 gatttatgat gtcctaaata acaactcaac caactttagc cactgggatg aagttggtgc
     4381 gtcatggaag ttgattgatc gtattgaaaa gctctgggct gaaaatggtg ccccacttca
     4441 tgactataaa gctggaagca tgggacctca agccagcttt gacctacttg aaaaattcgg
     4501 tgccaaatgg acttggcaac cagatatcgc ctatcgtcaa gatggtcgtt tcgaataaaa
     4561 aaatcctgca agtttgtacc ttgcaggatt tttgcttctg attagattaa gccttccaag
     4621 agacctttca taaagttttc tgagttaaac tctccaatat catcgatttt ttcaccaaaa
     4681 ccaatcaatt ttacaggaat attgagttct tcacgaatgg ctagaaccac acctcctcga
     4741 gcagttccat caatcttagt caaaacaatt cccgttaaag gtgtgatttt cgaaaattct
     4801 ttggcctgta ctagggcatt ttgacctgtt gatgcatcaa gtgccaagaa ggtttcatgt
     4861 ggtgcttctg gcacaacacg tttgataata cgaccaatct tttccaactc agccataagg
     4921 ttatccttat tttgcagacg accagcagta tcaatcatga gaatatcgat accttcagcc
     4981 acggcacgtt ccataccatc aaagaccacg ctggctggat cagctttttc aggtccagtt
     5041 actactggaa catctactcg tcggccccat tcagctagct gagctactgc acccgtacgg
     5101 aaggtatctg ctgcaaccag catgaccttc ttaccagttc gtttgtagcg gtgggctagt
     5161 tttccaatag aagttgtttt cccaacacca ttcacaccaa caaagagcat aactgtcaag
     5221 ttatcttgga agtggatgct ttcatcgtag ctaccatcct tttcataaag ctcaaccaat
     5281 ttctcaatga tgacacgacg aagtgcatca ggtttcttgg cattttcaag cttggcttcg
     5341 taacgtagtt cctccgttaa gttagaagcg acttggacac caacatcact cataatcagc
     5401 agttcttcca gttcctcgaa aaattcttcg tcaacagagc ggaagttagc aaagaaggcg
     5461 ttcaagcgag caccgaagcc cgtacgagtc ttcttgagac tgcggtcata tttttcctga
     5521 acagtttctt ctgtttgagg cctttctggt tcaagaactt cagaattatt ttcttctata
     5581 gttccttcgt gctcaagctt ctcttcctct ggtaattctt ctgagtttgg taattcttct
     5641 atttcttctt gagaaacccc tacagctggc tctgaatcct gactttcttc aactgtgtct
     5701 tggatttcct cctcttggaa cacagcttgt tcaacaattt caacctctgc ttcttcctga
     5761 ggaacttcat cagcttctgt gaaggtagga tcaacatctt cagacaaatc aagattttcc
     5821 agagcttctt ttacaacttc ttcgatttta ggttcttctt tttttccgaa tagacggtca
     5881 aacaatccca tatcttagtt ctcctttagc acatattctt cgatagccca ggcgacagct
     5941 tcctcatcgt tggtcatcgg cgttactaca tttgcggctg cctttacttc aggaacagcg
     6001 ttttgcatag caacaccaag acctgcccat tcaatcatag aaaggtcatt ggcctcgtca
     6061 ccacaggcca taacttggct ttggttgatt ccaagatggc tgattagttt tgccaaacct
     6121 gttgctttat gaacattctt tggtgaccac tctagcaaca tttcacgtga tttaaagatt
     6181 tcatattggt caaacaattc tggagaaatc ttctgaatgg ctgcatccaa gggttcttga
     6241 gcaaaggcgg tcacgcattt gttgtaggtc atttgattag acaggtcttc aaaatcaact
     6301 ggaacaaaag tcaaagctgg attgaatttg gcataaagac tttcttggtc cgattgaatt
     6361 tgataaacag ttccttctga gatagcgtca agaggcagtg ataatttctc tgtttcttca
     6421 tacaagcgtg ccacatcatc atatgaaaag actgtcttat caaggatttc acctgtattt
     6481 ttctgaacca aaccaccatt aaaagtaatg gtatactcat cttcctgacc gtcagtccct
     6541 agctcatgga gaaagaaatc catagctttt aagggacgac cagttgtcaa tacgaccttg
     6601 ataccacgat cacgcgcagc tttcaaggtt tccttggtac gatccgtcag ccttttatca
     6661 gtagtcagca aggtcccgtc caagtccaat gcgattaatt ttatatctgc cattataagc
     6721 cctccatata agctataacc gactggtcct tatggtgacc aatcacagtc tttgctaatt
     6781 ctaaaatttc aggtcgtgca ttttcaggag ctacaggatg tcccacaacc tgcatcatat
     6841 gtaagtcatt aagattgtct ccaaaagcca tgacctgatc cattgtgata ccaagttttt
     6901 taactaattc aacaatggcc actcccttat cgacatagtc cagaacaata tcaatagatt
     6961 caaagccagt tgtcatagcc ttaacaccag gaacgttttc gtttacccaa gcctccccat
     7021 cttccagcgt ttcttctgtg aagttggttg taaatttgaa aatgtcatct gtgatatctt
     7081 ccaaactcgc tactttttgg atattttcat tataatgctg actcactttc aaataggtct
     7141 catcaaccgt atctagaaca tatgaaccct tcttacccgt caagagcagt ttattgatat
     7201 ctacataagg tgacgttttc agcttttcaa aagttgccag ataaaagtca tgagacatag
     7261 tcgcttcata caagtcctga ccttgatact ctaccaaact gccattttcc gcgatgaaaa
     7321 taatgtcatc acgaacatca gcaaataatt tttccagaga cagaaatccc cgacccgaag
     7381 ccaccgcaaa gtaaatccct ttttccttgt aggaagccaa gagagacttg agacgatcca
     7441 tatcaaagcg tccattccca tctaggaagg ttccgtccat atccgttgct actagtttaa
     7501 ttgtcatcct tcaatacttt ctaaatcttt taacttaact gaaacaattt ttgagacacc
     7561 tgattcttgc atggtcactc catagataga atcagccgct gccatggttc ccttacggtg
     7621 ggttacgacg atgaactggc tgtccttgtc aaagcggttg aggtaatccc caaaacgttt
     7681 aacattagct tcatccagcg cagcttccac ctcatccaag ataacaaatg gaatagtctt
     7741 gacacgaata atggagaaga gcaaggcaag agccgataga gctttttcac caccactcat
     7801 gaggttaagc gactggattt tcttacctgg aggttgaaca gaaatctcca caccagctgt
     7861 taaaaggtcg ccctcagtca atatcaagtc tgcctgacct ccgccaaaca tctgcttgaa
     7921 ggtcacttta aaggactcac gaatagcttc aaaggttgat ttaaagcgtt ccttgacctc
     7981 atcattcatc tctgtaatgg tttcaaggag cagatttttc gctgacaaaa tatcatctcg
     8041 ctgactattt agaaagtcca gacggttgtg aacttcttcg taccggtcaa tagcttctat
     8101 attgacagga cccagtgagc gaatagcctt ctctaaatcc ttaacttctt gctctgccag
     8161 attgaggttt tccaattcat gcgctttttc tagagcttca gtatagctaa tctggtactg
     8221 gtctgttaat tgactttgta gatggcgcaa gcgttcgctg accttttctt tcttggcttc
     8281 agcacgtgtt tgcttgcgaa tccactcctc attctgctgg cgagcctgat ccaaatgact
     8341 ggcaatatca tccagttgac cttctatatc atccaactca aactgctggc gaatcaaacc
     8401 ttgttggaga tttgttttct gagttttgga ttcttccgcc tgttgactga gcaattctgt
     8461 atcaaccttc tcaagattat caaccttttc ttgaagaagg cgctggattt cctcttgttc
     8521 aaaatcaaga ttgtccaatt ccttgcctaa gcgttcaata tcagcaactt cataacgttt
     8581 ttgcccttgc agttctgtct taagcaaacg agcttgcgct agctcttcct gcaagttttg
     8641 atagcgttct tggatggcat ttttgttaga cttaatctct tcaatctcag cttccagatt
     8701 ttgcttgtca ctggcgattg cagcaagacg ctcttggcat ttttccttat ccgcttgcca
     8761 atctccctcg gaaagacgat ctatttcctc ttcttggagt ttccaaagag tttccagttc
     8821 ttcaacttgc tgactggtct gctgataggc gagggacaag ccttgctcct gaatacgagc
     8881 ctgctctccc tgagatttga tggcttctaa tctttctgtc aatctagcta gctcatcttg
     8941 caagttcttc aaactcactt catctgaacg caaacttgct tcttcttcag caatttcttt
     9001 ttgtaattgc tccagttctg gcttgatgaa aatactgttg ttctgacgat tggctccacc
     9061 tgcgtaagaa ccacccgtgc gtaattctgt cccatccaat gtcaccatac gaacctgata
     9121 acgaacttgg cgagctgctg cacgcgcatg ttctacggta tcaaagatag ccgtcgtagc
     9181 tagcaagttc ttgaaaatgg cttccagtct agtatcaaaa gtcaccaact catctgccat
     9241 cccaaggaaa cctgggctta cagcgatagc atcttggttc tgactagaaa tcgtacgcgc
     9301 cttaatagtg gtcaaaggaa gaaaggttgc acgaccggct ctgtttcgtt tgaggaaatc
     9361 aatggctttg gttgccgcat tctcatcttc tacaataatg tactgactgc tcgctccaag
     9421 cgcaatttct agggcagttt gataatgcat atcaaaggtc agatgctcac tgactgcacc
     9481 aataatccca cctaggcgat ctttttcttg gagaacactc ttaacacctg cataaaagtt
     9541 actatgattt ctcaggatat tttccaaact ttgagctctg gcctgcttgt ttttgagatt
     9601 atccagacgg tcaaagagtt ggctttgttg agcttgatag gaagttttct gctcctcttg
     9661 ctccttggca atagcttggt agtcagccaa taatttctga acctgctcct tggcagtttc
     9721 aagctcttcc ttttgctgac tagccttctc tttagctata gctaattgct ctttcagctt
     9781 ttctagttga tctgcttgtt tttgagaaag ctgacgacta ttttccaact cattctcaat
     9841 acgggtcaac tggtttgaga catccgcttc ttcttgtaaa agagctacaa agcgttcacg
     9901 taagagctca atcatctgat caggatcgtc tgagaaagcc agcaattcag cttctaaacg
     9961 attgagtttt tgattatttt ggactagatt tccctctaac agagctaaag agctttcttt
    10021 atcagacttt tctttgctga gtgaatttct cttatcctcc aaagcagcca aacgggcttg
    10081 tgcctcctgt tgattcaagg ccacttgctc ggactccagt ttcgataggg ctaattttct
    10141 ttctaaatca ctaatcagac tagtcaagtc catcaaactg ccttggtctt tggccatttc
    10201 agcctgtaaa tcttggcgtt gctttttaag agtttgattt tcttcttcta atttttcacg
    10261 cttttggtaa taactcatca agagttcttg aacctgagtc aactcttctt ctgtcgactc
    10321 tagttcagcc ttattttcct tgatttgagc aaccagaaca tctaaataaa tagccttacg
    10381 ttgtccttcc aagtctaaaa acttacgggc attctcagct tgcttctcaa gaggcttgat
    10441 ttgattatcc aactcgtaga taatgtcctc taagcggtcc agattatcct gagtttgttg
    10501 cagtttactc tcagtttctt ttctacgagt cttgtatttt aaaactcctg cagcttcttc
    10561 aaaaatagct cggcgttcct caggcttaga attaaaaatc tcctcaacct tgccttggga
    10621 aatgatagag aaggaatctc gtcccaatcc agtatccaag aagaggtcat gaatatcacg
    10681 cagacggact ttcttgccgt caatcttgta ttcgctatct ccactacgat agatatggcg
    10741 ttccaccctg atttcttgac ctgcatcctt gataaatccg tcatgattat ccagagtcac
    10801 aactacagaa gcataattga gcggtttgcg actttcggtt ccagcaaaga tgacatccgg
    10861 catcttgccc ccacggagac tcttgacact agactccccc aaagcccaac gcagactttc
    10921 tgtaatattg gactttccag atccattggg tccaacaact gccgtcacac cttggtcaaa
    10981 aacgaccttg gtcttatcag caaaagactt gaacccctga atttcgattt cctttaaata
    11041 catgaatcca gccccttctc aacggcattt ttggcagctt cctgctctgc taatttctta
    11101 gaacgacctt ggccttgacc gatgctctta ccttcaacaa gaacttctac atcaaagacc
    11161 ttatcgtgag caggccctgt ttcagaaatc acctgataac gaatagccac atcaccattg
    11221 acctgaagca actcttggag atgggtttta tagtctgtaa tcatctcaaa ctcgcctgct
    11281 tcaaccttag gaatcatgac ttgatagata aattccttga ccttggccac atccttatcc
    11341 aaaagaaggg caccaagaaa ggcttcaaag gcatcaccaa gaatggtgtc acgattgcga
    11401 ccaccagatt tttcttcccc tttacccaac ttgataaact ggtcaaactg gcaatcacgc
    11461 gcaaaaccag ctaaactctc ctcacggaca atcatagcac ggagttttga taggtcacct
    11521 tcaggctttt taggatattt tttatacaga tattctgaaa tcaataactg tagaacagcg
    11581 tctcctaaaa attccaagcg ttcattgtgt gaaattttta agaggcggtg ctcattggca
    11641 taactcgtat gagtaaaggc agtttccagt aaatttttgt ctgtaaattc gattgcaaaa
    11701 tggttcttta gtacagtttg taattctttc ataccaacct ctttctaact gataacagtc
    11761 ctttttatta tatcaaaaaa agccccctga gtcactctaa aacgggactg gaaagcattt
    11821 gggaattctt tagacagaga ttctcagttt tagcggcaaa tttagatcag tgtaaagaaa
    11881 aaagccctat taaaggcttt ttaggatgtt tacatccacc ctgagggaat cgaaccccca
    11941 tctcaagaac cggaatctta cgtgatatcc attacactaa gggtggaaac ttgttttatt
    12001 ataacagaaa tttgctctaa taacaagttt tttagtcaaa gcccccgtct tagtgggaag
    12061 catccccatt ccagatggag tttttcacga tcacataatc aacgtgttta aggtcagcaa
    12121 cctgacgtcc acctgcataa gaaatagcac tttgaaggtc ttgttccatc tcagttaaag
    12181 tgtcttgcag atgaccttta gcaggaagca agatacgttt gccttccaca tttttgtaag
    12241 cacctttttg atattgtgag gctgaaccat aatattcttt gaactgttca ccatcgactt
    12301 caatcgtttt ccctggactt tcaatgtgtc ctgcaaagag ggaaccaatc atgatcatgc
    12361 tagcaccgaa gcggatagac ttggcaatat caccgtgagt acgaattcct ccatcagcga
    12421 taatcggttt acgcgcagcc ttggcacacc agcgtagagc agccaactgc caaccacctg
    12481 taccaaaacc agtcttaacc ttggtgatac aaaccttacc aggaccgatt ccgaccttag
    12541 tagcatccgc accagcattt tccaattcac gcacagcttc tggtgttccc acatttccag
    12601 caatgacaaa ggtatctggc aattctttct tgatgtgttg aatcatagaa atcacgctat
    12661 ccgcatgacc atgagcaata tcaatagtga tatactcagg agcatcagtc ttgagctgtc
    12721 taacgaaatc atactcatag tccttaacac cgacagagat agaagcaatg agcccttgat
    12781 cgtgcattcg tttaataaaa ggaatgcgtc ctgcctcatc aaaacggtgc ataatgtaga
    12841 agtaaccacc tttagccagt tgctctgcta cattttcatc caaaatcgtc tgcatattcg
    12901 ctggcacaac aggtagttta aaggtgtgat ttcctaaagt gacacttgta tccgcttctg
    12961 cacggctttt aatgacacat ttatttggaa tcaattgaat atcttcgtaa tcaaaaattg
    13021 gaaattcatt taacatatcg atgtctcgtt tcttttgtaa tgacctacct atgctctcgc
    13081 atcactacgt cttttccgac gtttccttaa tttattataa accaaagtac agtttttgtc
    13141 aaattatttt atgaattaaa atatatcgtt cgggttttac ttgtgttaaa aataaaagaa
    13201 agaggggaaa ttattttctc cccacttctt catcaataat cgctttaaaa ttttcttctg
    13261 aattttccat cattttacaa aatgcactat aggatgaggc attctgaggg attcgaatag
    13321 accacttctt caattgagct ccatagccag ctacttttcc tatttcacta tttactgact
    13381 gctccatact aggaaaaatc agtccagcct tctcagtttt taaaatataa gaataggaaa
    13441 tcagctggaa taagtcctca cggttgattc ctttttcagt caattccagt tttttatatt
    13501 ttgcatctag aacaatcttt cgttctctgt cataaaaatc tggatatact tttcgtttcc
    13561 caacaaaaaa tactgaaatt ccatccgtct tatccttatt tctgggatgt acaaaacctt
    13621 ttggcaacaa ggtgtaaaca tactcttccc aaagccaggc aacatcaaag agaataccat
    13681 agattttttg atcttgatac cctaaaccgt gcttttcttg gtttaggatc atcagacaaa
    13741 gttcttgtaa gtttctgtac tcgtgaaagt atgcatgacg tataggttta gattgatttc
    13801 cccgaataat cttagcacga tcagctagtt tataagaggg cgttacacgc acgatttcag
    13861 atacgttttc acgactagtt gagagattat ctagtacccc ttgaccaatg cttttctgat
    13921 tcttaatgta ttcaatagtg tgacggacca actgcatgag gggattatca taggtgaact
    13981 ctctcgttgt gtaggcaata tttcccgtga aaggaagatt tttcttgaga tggtttctta
    14041 catcaatcac tcccttaaca tgactgtcgt tatgagaaaa tcgatgatat tccttataaa
    14101 gaccttttcg aatagcggct tgtagatact tggggaagag atacatcaaa agttgataaa
    14161 gcctctcttc acgagacaaa gcaacatcta aactagtgag attgatataa agaaccttgt
    14221 ttaagagata atgcaaaaaa tggtcattac tctcatcaga aaatcgtgag gaaatcgtta
    14281 atctttcctg accatatcca agaaaaccaa tcacgttccc tgtcttgatt ttctgattga
    14341 ctgtttcaaa aatcttttgg tccttatcca aatcaggagt atgagtcaaa tcatttggaa
    14401 aaatgaaaat acgttcatct tgggatagac tttccaaggt tctgtctaaa aggagattgc
    14461 ttagtttagg atattcttca acaaattttt ctttaataat cttgtgttga ttatcagtaa
    14521 tccgcatcat cgttttcatc accttcattg tcatcagcta ctgcctgatc tttttgctca
    14581 ttatttgtca gttcaaatgc ttttttcaaa gtttccaaag tttcaacctc atcataagaa
    14641 cctcgcaagt agtcttctag gagaggctta atataatcag accagagtaa ttcatagtca
    14701 aaatctacat ccttcaactt aagaaaataa cttggtccaa tatgataatg actgtttaat
    14761 tcctgaacat tttcgatagc agcgttcaag tttcttagac gaatttttgc ttcttctgca
    14821 tggatattca gctctttatc caacatgcca acttgaccct cggcagtaac ttcaacaaaa
    14881 cgaaaacgac gacgcatagc aaaatcaaag gtatccactg aacgatcaat atcattcata
    14941 gttccgatga tgtaaacatt ttcggggata tagaactttt catcagtttc gtgtagattt
    15001 gcatattggg tagaaacact tcctttttca ccacgatagc cagggtcgat agagaaaaag
    15061 agttcgccaa aaatcttaga aatctcccca cgattgattt catcgatgat gaagacgaat
    15121 ttcttatcag tatcaatttc tgttggggaa acatagtctt tcaaaccaaa tctctttctc
    15181 aatgtttcta ggacagtttt tccaccactt ttgtagtatt cttgcttatt ataattttta
    15241 tctttataca actcgtaaac atatttgcta ggtagtgacc atcctggaac accactatca
    15301 taatttactg acaaattttg tctactatta acagataagt aagatgtttt tgttatatat
    15361 tctttttctt cagcaacatt tatatattct aagtaagaat cccaagcctc atcaaaatta
    15421 tcttgtcctc caatcaattg ggtttctttt gctttctgac aaaaatcttt aaaaataccg
    15481 tcctgtagcc taaactcaat agctccatcc ccatttgata ctggtcttaa accttctaca
    15541 aaatccgtat aatcatatga tgggtgaaat tgtacaaatc cgatttggtc ttcgttgcca
    15601 tccgttaatt ctttggcaat ttctttagca agataagtct ttcctgtgcc aggagcacca
    15661 cgaaggataa ggtttggaga ctttaataga gattgagtta attgatgacg aaaattgttt
    15721 gatgaatcca ttgtactatt gtcttcctta ttttccataa aaagcttaga atactcaaaa
    15781 agcatagttc ctaatttcga tggctccaac tcaggataag ttttggacac cagttcaatt
    15841 attttatgat tttgcagata aggtccacct tgatttgtct gagaaacaaa gtggttccaa
    15901 aattctttct gactattaat atttacacca aagaaaggat ctccctcaga atagatacaa
    15961 agtaattttg tcaccatagc agaacgac
//
LOCUS       DAVHIU010000038        15815 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVHIU010000038 DAVHIU010000000
VERSION     DAVHIU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.040"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(1..317)
                     /locus_tag="VVN06_001027"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="VVN06_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..900
                     /locus_tag="VVN06_001028"
     CDS             442..900
                     /locus_tag="VVN06_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU5562595.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            913..1521
                     /locus_tag="VVN06_001029"
     CDS             913..1521
                     /locus_tag="VVN06_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562596.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1592..1924
                     /locus_tag="VVN06_001030"
     CDS             1592..1924
                     /locus_tag="VVN06_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562597.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQSRLISLNKSEF
                     MQLNPGTY"
     gene            1935..3350
                     /gene="ply"
                     /locus_tag="VVN06_001031"
     CDS             1935..3350
                     /gene="ply"
                     /locus_tag="VVN06_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU5562598.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4231..4947
                     /locus_tag="VVN06_001032"
     CDS             4231..4947
                     /locus_tag="VVN06_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5562599.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            5071..5520
                     /locus_tag="VVN06_001033"
     CDS             5071..5520
                     /locus_tag="VVN06_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU5562600.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            5559..6380
                     /locus_tag="VVN06_001034"
     CDS             5559..6380
                     /locus_tag="VVN06_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562601.1"
                     /translation="MILLAILFTCFLVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYISRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVVVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIIFMSYAM"
     gene            6387..7418
                     /locus_tag="VVN06_001035"
     CDS             6387..7418
                     /locus_tag="VVN06_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5562602.1"
                     /translation="MGFLLMGALFIVFPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSVTAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKRSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIACALLKDASLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSIIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7484..7846
                     /locus_tag="VVN06_001036"
     CDS             7484..7846
                     /locus_tag="VVN06_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU5562603.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7843..8346
                     /locus_tag="VVN06_001037"
     CDS             7843..8346
                     /locus_tag="VVN06_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5562604.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8460..8906
                     /locus_tag="VVN06_001038"
     CDS             8460..8906
                     /locus_tag="VVN06_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5562605.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8911..9585
                     /locus_tag="VVN06_001039"
     CDS             8911..9585
                     /locus_tag="VVN06_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562606.1"
                     /translation="MKKKAFGIVFLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWCVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            9837..10025
                     /locus_tag="VVN06_001040"
                     /pseudo
     CDS             9837..10025
                     /locus_tag="VVN06_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            10275..10574
                     /locus_tag="VVN06_001041"
     CDS             10275..10574
                     /locus_tag="VVN06_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU5562607.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10571..10888
                     /locus_tag="VVN06_001042"
     CDS             10571..10888
                     /locus_tag="VVN06_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5562608.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            10904..11530
                     /locus_tag="VVN06_001043"
     CDS             10904..11530
                     /locus_tag="VVN06_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU5562609.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11563..12324
                     /locus_tag="VVN06_001044"
     CDS             11563..12324
                     /locus_tag="VVN06_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5562610.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIETLQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            12402..12797
                     /locus_tag="VVN06_001045"
     CDS             12402..12797
                     /locus_tag="VVN06_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5562611.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12953..13237
                     /gene="groES"
                     /locus_tag="VVN06_001046"
     CDS             12953..13237
                     /gene="groES"
                     /locus_tag="VVN06_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5562612.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13253..14875
                     /gene="groL"
                     /locus_tag="VVN06_001047"
     CDS             13253..14875
                     /gene="groL"
                     /locus_tag="VVN06_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5562613.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            15013..15768
                     /locus_tag="VVN06_001048"
                     /pseudo
     CDS             15013..15768
                     /locus_tag="VVN06_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4724 a         2874 c         3630 g         4587 t
ORIGIN      
        1 ttgaaaacat tttaaacgtt tttactttgg taaagatgtt ctcaaccttg cttctctcct
       61 tagatagcgc atggttacag gatttatctt caactgttag cggcttgagt ttgctggatt
      121 tacgtggagt ttgtgcttga ggatatatct tcatgagccc ttgataacca ctgtcagcca
      181 agattttacc agcttgtctg acttttttga taagctgttt ttaatacagc taacatctct
      241 tcaaaagtgg tacgctgaac accaacaaga cgcttaaatc gtgcatcagt tagttgttta
      301 cttgcttcat aattcataga actactatac catattttgt ttcgcaggaa gtctaataag
      361 ttatagtcca gtttttacta tactttgata ttcaaatatg ttatactatg gttatcaaag
      421 taaatgaaag gagttacgaa tatgacgcca gaagaaatgt acctgacaga gcgattagac
      481 gtacagatag ctcatttttt aaagaaaagc gttcaacatc gtaggcgcta taaggtatta
      541 aaaataacag aaatcgtggc aggtttcctc atagctgtct tttgtgctat tcctatgcca
      601 ggtgatcgct accgtttgat ttcggttgcc ttatccagtc tcggcttgct gtgtgagggg
      661 attatcaatt tgtataatgc aaaggaaaat tggatttctt accaaaaaac tgcgcaactc
      721 ctggagaaag aaaaattcct ctatcaatgc caaacggaga aatatgcagg aaagaccaag
      781 gcttttgccc tatttgtcaa gacatgcgaa ggtcttatct cagaggagat taaccagtgg
      841 gaaagtatcc agtcaaaaga agtggcagct agtgcagatg ctccagttaa aaaagagtag
      901 gaggtagagg aaatgtctca atccagctac ctgtcgccct tgctctggtt aaaaaaagaa
      961 gccgataagg aaaagatgag cgcgacccag tgccagatat ttttctttta ctatcaaatg
     1021 tttgagctct tatttgctag agaaagcgac atgaaagact tatgtctggg aacgaaaggt
     1081 ttttatttct cgcagttaga gaaaaatttg ctttctggag tttcccgatt tctaaaaaac
     1141 ttggagggga aagtaactct caaggctaac caagaagtat cagctcgcaa agcccttttt
     1201 ctagccttga caactagcca atcagattgg caggagttag ctcctgtttt tgatttttat
     1261 cagactatcg ggaggcttga aaatccttct ctcttgagtt ctcaggacag acaacatctg
     1321 atgtggattt accagtcagc tttggagaag gattatattg tcaaggttat tggtgacaag
     1381 cattttgtat tgaagagaca agatgctact aaattgacag cgcgccaaac tcaaactttg
     1441 gaaattctga gtcaatcaga agacttggtc aatcctgtct atgttacatt aggagaaaag
     1501 ggggtgctct tgcttgatta agagaggaga tgttgtagtt ctttatttgc cttttccgac
     1561 tattagtagc gatttggctg tgaagaatca tatgtatatc tgtattgaca acagcatgac
     1621 taaaaacaaa gagttggtta aaaatcagac cttcaaacca gctctcttga ccagacgttt
     1681 ggtcaagaac tttatgatag aagagccgga tctagctcgt aatcctttta caagaccaac
     1741 cttgattgac ttagataagg tatttatgtt ggataatacg gttattccga cttcttatct
     1801 agccagacgg cgacgcaatg tctcagaaga attgtacgag gaaattttgg atcacttagt
     1861 ccaatcacgg ctgatttcgc tgaacaagtc tgagtttatg caactcaatc caggaactta
     1921 ttaggaggta gaagatggca aataaagcag taaatgactt tatactagct atgaattacg
     1981 ataaaaagaa actcttgacc catcagggag aaagtattga aaatcgtttc atcaaagagg
     2041 gtaatcagct acccgatgag tttgttgtta tcgaaagaaa gaagcggagc ttgtcgacaa
     2101 atacaagtga tatttctgta acagctacca acgacagtcg cctctatcct ggagcacttc
     2161 tcgtagtgga tgagaccttg ttagagaata atcccactct tcttgcggtc gatcgtgctc
     2221 cgatgactta tagtattgat ttgcctggtt tggcaagtag cgatagcttt ctccaagtgg
     2281 aagaccccag caattcaagt gttcgcggag cggtaaacga tttgttggct aagtggcatc
     2341 aagattatgg tcaggtcaat aatgtcccag ctagaatgca gtatgaaaaa atcacggctc
     2401 acagcatgga acaactcaag gtcaagtttg gttctgactt tgaaaagaca gggaattctc
     2461 ttgatattga ttttaactct gtccattcag gcgaaaagca gattcagatt gttaatttta
     2521 agcagattta ttatacagtc agcgtagacg ctgttaaaaa tccaggagat gtgtttcaag
     2581 atactgtaac ggtagaggat ttaaaacaga gaggaatttc tgcagagcgt cctttggtct
     2641 atatttcgag tgttgcttat gggcgccaag tctatctcaa gttggaaacc acgagtaaga
     2701 gtgatgaagt agaggctgct tttgaagctt tgataaaagg agtcaaggta gctcctcaga
     2761 cagagtggaa gcagattttg gacaatacag aagtgaaggc ggttatttta gggggcgacc
     2821 caagttcggg tgcccgagtt gtaacaggca aggtggatat ggtagaggac ttgattcaag
     2881 aaggcagtcg ctttacagca gatcatccag gcttgccgat ttcctataca acttcttttt
     2941 tacgtgacaa tgtagttgcg acctttcaaa atagtacaga ctatgttgag actaaggtta
     3001 cagcttacag aaacggagat ttactgctgg atcatagtgg tgcctatgtt gcccaatatt
     3061 atattacttg gaatgaatta tcctatgatc atcaaggtaa ggaagtcttg actcctaagg
     3121 cttgggacag aaatgggcag gatttaacgg ctcactttac cactagtatt cctttaaaag
     3181 ggaatgttcg taatctctct gtcaaaatta gagagtgtac cgggcttgcc tgggaatggt
     3241 ggcgtacggt ttatgaaaaa accgatttgc cactagtgcg taagcggacg atttctattt
     3301 ggggaacaac tctctatcct caggtagaag ataaggtaga aaatgactag gagaggagaa
     3361 tgcttgcgac aaaaagaggc gatgatctct ctgcagatat ctgaaagttt atctcttgcc
     3421 tagcgatttc cattggagta tgctcgtgat gttaaaatga atctatcaaa cagaatgtgt
     3481 aaaaaagtaa aaagggtagc caattctttg atatacaggg gattgattat cctttacttg
     3541 ttttagatga ttggaaattc aagaacgaca cgtatcggaa atattttgct gtttatatct
     3601 aatacccatt tatggaaaaa atgaaattaa atatgaacaa atcaattgct agcaatggtt
     3661 tagaagtgcc agtgtactag tctaacttct ccatcttggg aacacgtaaa ttatactctt
     3721 cgaaaatctc ttcaaaccat gtcagcccca tccgcaacct caaaacagtg ttttgagcaa
     3781 cctgaggcta gctttctagt ttgctctttg atttttattg agtattagag gtttgcttag
     3841 aaactcttct attgatttct tagaatacaa tagcgcctat aagaactgtt gaatttcaaa
     3901 gtatttactt gaactatgat atgtctggag tagactgtca acaaaaggtg cagtatgatt
     3961 gtttttgtcg cttatatcta caaacttcaa acagtaattt gagctgactt tgtcaaactt
     4021 ggctgaaact ttctatttgc agataatggt ctagtttgtt ttttgaatgt ggatgagtgt
     4081 ctgttgataa agatagaaag gcaagaccat cttagcaggt aaagtgctat cctagcttga
     4141 tttgctaata aaatccattt cctctagtga aaatcaaaaa aacttgtgtt ataataagaa
     4201 agattaaaat gtgaaaaaag gagattccta atgggacgta aatgggccaa tatcgtagcc
     4261 aagaaaacgg ctaaagatgg agctaactct aaagtatatg caaaatttgg tgtagaaatc
     4321 tatgtagcag ctaaaaaagg tgatccagat ccagaatcaa actcagcttt gaaattcgtt
     4381 atcgaccgtg ctaaacaagc ccaagtgcca aaacacatta tcgataaagc gattgataaa
     4441 gccaaaggaa acacagacga aacctttaca gaaggacgtt acgaaggttt tgggccaaat
     4501 ggctctatgc taattgtgga tactttgact tctaacgtta accgtacagc agccaatgtc
     4561 cgtgcagcct ttggtaaaaa cggcggaaac atgggcgctt caggttctgt ttcttacctc
     4621 tttgacaaca aaggtgttat tgtatttgga ggtgaagatg cagacgcagt ctttgagcaa
     4681 ttgctcgaag cggatgtaga tgtggatgac gtagaagcac aagaaggtac aatcacagtt
     4741 tacacagcgc caactgacct tcacaaggct atcgttgccc tccgtgagtc tggtattgaa
     4801 gagttccaag tgactgaatt ggaaatgatt cctcagtcag aagtggaatt gtcaggcgaa
     4861 gaccttgaaa cctttgaaaa actttacagc gttcttgaag acgacgaaga cgtccaaaag
     4921 atttatacga acgtagatgg gttttaattt aacctataga acatgatcct aagtgagaaa
     4981 atcacttggg atttttttaa tcaagaaaat agttctcata gaaatttaat tattctccag
     5041 agaactattt ttatttagaa gggggagatt atggataaac cgatgttagt ctttaagcgt
     5101 tttggtcatc aaattcacct gatggtgcaa aaggaagcca aacgttgcgg cattgaattt
     5161 atgggtgggc cgcaaggtca ggttgtgcat tttttggata atcgcgagaa aaaccaagac
     5221 ttggtcttga ttaaagatat cgagcaggaa ctcaatatta ccaagtctgt tgctagtaat
     5281 ctggttaagc gtatagtgca aaatggtttg gtggaattgg aggcgagtcc tgttgataag
     5341 cgggctaagt ttgttcgtct gacggacaaa gcacgttctc agatgcaaca ggttaaggct
     5401 ttttttgaac gcatagacaa gcagttgatg gaagacattg atgaagatga attactgatt
     5461 tttgagaagg ttctcggtca actacaggca aaatatcaag ggaataggag gagagaataa
     5521 agaaattagc caaaagaatt agtagaaaag aatgggggat gattttacta gccattctct
     5581 ttacttgctt tttggtctat ctagagttgg aagtgccgac ctatatctcg aaaattacgg
     5641 atttgctagg tagtcaagaa actaatttag atgagttgtg gcagccggca agcatgatga
     5701 tgggaatgtc ctttcttgcc ttcttgtccg tagttgcagt tggatttttt gcatcccgag
     5761 tggcggcttc ttatattagt aggctgagaa gtgatatttt taaccgagtt ttggattact
     5821 cgcagacaga gattaagaaa ttttcaattc ctagcctctt gacgcgtact accaatgaca
     5881 ttactcaagt tcaaatgttg attactatgg gcttgcaagt ggtaacgcgt ggtccaatta
     5941 tggctatctg ggctattggg aagattttag gtcattcaga atactggctc tgggccgtac
     6001 ttgtggtagt gattgtcaac gtcctgatga cgaccgtttt gatgacgcta gcctttccaa
     6061 aacagtcctt gattcagggg ttgacagata aactgaacag tatcactcgt gagagtttaa
     6121 caggtattcg tgtcgttcgt gcctacaatg cagaggatta tcaaaatgaa aaatttgcag
     6181 cagtaaatga tgaattgacc cgtttgaatt tgtttgtcaa ccgtcttatg gctattttga
     6241 atcctatcat gatggggatt tcaagtggtt tgagtgtagc gatttactgg attggggcct
     6301 atgtgattaa cgacgctgct ccgatagcgc gtctgcctct ctttagtgac atgattattt
     6361 tcatgtctta tgccatgtag gttgtcatgg gcttccttct catgggagca ctcttcatcg
     6421 tttttccccg aactatggtc tctgctaagc ggattaatca agttttagat ttgcattctt
     6481 ctatccaaaa ccctgttcaa gtgcagctga ctgatgaaaa cttcaaaggt caggtcgagt
     6541 ttaaggatgt gaccttccgc tatgcggcaa attcggaggc agttattgaa catgttagct
     6601 ttaaagcaga aactggtcaa acagtggcct ttattgggtc aacaggttct ggtaaatcaa
     6661 ctctggtcaa tctgattcca cgtttctacg acgtgtcagc aggagaaatt ctggtggacg
     6721 gtgtcaatgt tcaagactat gacttctctg tgacagctca tgctggtcaa agggttgcca
     6781 ttgttgggcc gactggggct ggtaagacaa ccattgtcaa tcttttgatg aaattctatg
     6841 agattgataa gagaagtatt cgcattgatg gtgtggatac caaggctatg acgcgttcag
     6901 aagtgcatga tgccttttca atggtcttgc aggatacctg gctctttgaa ggaactattc
     6961 gagacaatct catctataat caaataggga ttagtgatga acgaatgatg gaagctagta
     7021 aggctgtggg aattcaccac tttattatga ccttgccaga tggctatgat accatcttgg
     7081 atgacaccgt gaccttgtct gtaggacaaa aacaactatt gactattgct tgtgcccttc
     7141 ttaaggatgc atcgcttttg attttggatg aggcgacttc ttctgttgac acacggacag
     7201 aggaattgat ccaaaaagcc atggaccgtt tgatggaagg acgcacatcc tttgtcattg
     7261 cccaccgctt gtcaatcatc cgaaatgcag acttgatctt ggtcatgaaa gatggaaata
     7321 tcatcgagca aggcaactat gaggaactga tggcgcaagg tggcttctac gctgacttgt
     7381 acaatagtca atttacagaa gatgaagcag aagaataaat caaaaaaagg cttgacggcc
     7441 ttctttttct tatttatact agtacacata gaaagagaga agtatgagtc agaaattata
     7501 caatatgaaa tttgctgctg tctacttagc cttgattgca aaagttgagc gaaagggtgg
     7561 aaaggcagag tcagttcacc aagtgaccag ttggttgaca gggtatgaag tgagtgacgt
     7621 tcttgcttgt ctggataggg atgttactta cggagatttt tttctacaag caccatatta
     7681 tgtccctgaa cgaattgcaa ttacagggaa gatttgcggt gtccatattg aggaaattga
     7741 tgatcctctg atgcaggaaa tacgtcgttt ggacaagcta gtggattggc ttgccaaggg
     7801 gaaaacatct caacaagttc tagaaaagta tgagaagcat aaatgaaaaa ttatcaagaa
     7861 tggtatcgaa atatcagctc cagactaact agtcgtccaa ttcttttatt cctattacgc
     7921 agtttcaatc gtttgatgac agtcgccatg cccctggtct atctgatctt gctagtcacc
     7981 acttacctgc aactaggact tggtcagcaa gttggggttt atgtgcttat tcctgcatca
     8041 ggttttgtgg ccttgtccct gtttcgtaag aaaatcaatc acccgagacc ttatgaaact
     8101 tgggatatct gtccccttct tgataaggat agttcgggac ggtcgatgcc cagtcgccat
     8161 gtcttttctg caactatcat ctccatggcc tgtctgcatg ctagtctacc tattggctta
     8221 gtatgcttga tcttttcagc tttgctgggg ttggtgaggg ttttaggggg tgttcattat
     8281 cccaaggatg tcttggttgg ctatgcttgt ggtctggtgt ggggattcct tttcttccta
     8341 ttctgacatg taagttaagc tagtcctaca accacttact gcttcaaatt gaccacttgc
     8401 ttttttgccc ttgtattcct aacttagctt tgatatagta gagtcagaaa ggagatgtga
     8461 tgaagttacg aattgagatt gacggcaatt tagaggagac tgaaattgtc atcaagaccc
     8521 ccactttgac agatgaaatt gcagacttgc aacggctctt gcaagagtca aaggctccga
     8581 ggttgacttt ttacaagggg acaggtgaat attatctaga cctgtcagaa attctcttct
     8641 ttgaaacaga agggagcaag atctacgctc ataaccagaa ggaagcttat gaggttcgcc
     8701 tcaagctcta tgagttggag tctatcttgc ctcgctattt taatcgagtt tccaagtcaa
     8761 cgatcgcaaa catccgtcag atttactcag tggacaagtc cttttcagga acgggcacca
     8821 tttcctttta tcagacgcac aaggaggttc atgtctcacg gcattaccaa tccctcctaa
     8881 aagaaaatct aagaaacatg aggtaataag atgaaaaaga aagcatttgg tattgtgttc
     8941 cttgtactag cagctttaat attagtacaa ggaaattttg gaatcccttc tctaggaggg
     9001 cacatttggc ccttgattgg tattgctttt tttgcctacc aatcagttgg agctttgttg
     9061 cgtcgccact taacttcagc ctcttttaca gctctagtag ccttaatgat tgctaaccac
     9121 ttttatggca ttttaccgat tccaaaccag acactaatct gggcaggagt tctaatcgtt
     9181 ctcggagtaa atatgttgac tcattctaac agaacttgga atgggaaaaa atggtggtat
     9241 gatggtgaaa aaaacattct tatagataag gaagttgctt ttgggacagg gactttctac
     9301 aagcaagacc aagaattggt agaagaccaa gtagaagttg gttttggcaa tgcaaaaatc
     9361 tactatgaca atgcagagat gttaggtgat tttgcaactt taaatattga agtagctttt
     9421 ggtaatgcaa ctgtctatgt tccgcaacac tggtgtgtcg atttgaaagt agaaacatcc
     9481 tttggtgcag ccaaggcgga cgctcctgta gcgccaacaa gcaaaacctt gattattcgt
     9541 ggagacgtgg cctttgggaa gttggagctt gtctacgtta aataaaaaaa tcttcactta
     9601 aaccatcaaa atagacgtac taagagtagg aaattgatgc cttgctctga tttcagttct
     9661 atggttgtta gactttaaaa aatgaaatgt tgcctttaaa agttgtatat ttttcgatat
     9721 tttggctctt acgtttgatg tatctatgtc ctacagcgtg gatgatgtag tgtaaaatgc
     9781 tttaaaaaaa tgaatgatat tggacagttt tttgaacctt taattgctca ggagccatga
     9841 aagcaagcac ttgagtttat gacaagggag aatggtatta tgtaagttct tctggagcca
     9901 tgctagcgaa tgattgggtc aaagataatg gcaagtggta ttatctagct tcatcaggta
     9961 agatgcttcg caatacctac acacctgatg gttactacgt tggtaactca ggcgcctggc
    10021 aataagaata atactcttcg aaaatctctt caaaccacgt cagcttcatc cacaacctca
    10081 aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag
    10141 cagcctgcgg ctagcttcct agtttggtct ttgattttca ttgagtataa gaagcccttt
    10201 tccttaaagg aagaggcttt ttacttgctt ttttgctgtt tcctcatttg tcctaaagcc
    10261 ttttttgtgt taaaatgaat ggtatgaaag ctaaaaaaat gtggatggca ggtctggctc
    10321 tgcttggtat tgggagcctt gcccttgcta cgaaaaaagt tgcagatgat cataagctca
    10381 tgaagactca ggaagagttg acagcgattg tacgagacca tttttccgac atgggggaaa
    10441 ttgcgaccct ttatgttcaa gtttacgaaa gcagtctgga gagcttggtt ggtggcgtca
    10501 tttttgagga tggccgtcat tatacctttg tctatgaaaa tgaagaccta gtctatgagg
    10561 aggaagtctt atgatacagc cagcaagttt agaagaatta gcatctttag tggaaaaagc
    10621 gggcaagaag gtcttccttt ttgtggcaga ctggtgtggc gattgtcgtt atatttatcc
    10681 tgccttacca gagattgaag agaccaatcc agagttcacc tttattcgaa tggaccgaga
    10741 tcagtatatg gatttggcca aactctggga tgtttacgga attcctagcc ttgttgttct
    10801 agaaaaggac aaggaaatcg gtcgttttgt caatcgcgac cgtaaaagta aggagcaaat
    10861 taacgatttt ttagcaggat tgaaatagga gaaaaaggaa acaatgattt ttacgtataa
    10921 caaagaacat gtcggtgatg tccttatggt catcgtgaaa aatagcggag atgccaaact
    10981 gaatgtggaa cgcaaaggca aggtagcccg tgttttcctc aaagaaaatg gggaaacagt
    11041 agcttggaac atttttgagg tttcgagctt gtttgaaatt gcagagcgcg gtcaagtctt
    11101 tttatcagat gagcaagtcg ctcgtttgaa ccaagaatta caggcggaag gttttacaga
    11161 agaaattgtt aatgataagg aacctaagtt tgttgttggt gaaattgtcg agatggtagc
    11221 tcatccagac agtgaccacc tcaacatctg ccaagttgca gtcgcaagtg acaagatagt
    11281 gcaaatcgtt gcaggagcac ctaatgcgcg tgttgggttg aaaaccattg tggctcttcc
    11341 tggtgccatg atgccaaaag ggaatttcat tttcccaggc gaacttcgtg gtgaaaagag
    11401 ttttgggatg atgtgtagtc ctcgtgaatt gcatttgcca aatgctccgc aaaaacgtgg
    11461 ggtgcttgaa ttatcagaag accaagttgt cggaacaccg ttcgacccag cgaaacactg
    11521 gactgcctag gaacttgtca gcatctgata gaaggaaata agatggcaaa aaatgtagtg
    11581 attacgggag caacctcagg aatcggtgaa gcgattgcgc gtgcttatct ggagcagggt
    11641 gaggatgtcg ttctaacagg acgacggata gacagattag aaatcctcaa gtcggagttt
    11701 gcagtaagct ttccaaatca aaccgtctgg acttttccac tagatgtgac ggatatggtc
    11761 atggtgaaga ctgtttgctc tgatattcta gaaacgatag ggaggattga tatcttggtc
    11821 aacaacgccg gactggctct tgacttggct ccctatcaag actatgagga gttggatatg
    11881 ttgaccatgt tggataccaa tgttaaaggt ctgatggcgg ttactcgctg tttcttgcca
    11941 gcaatgataa aagtcaatca aggtcatatt atcaatatgg ggtcaaccgc aggaatctac
    12001 gcctatgctg gtgccgctgt ttactcagct accaaggcag cggttaagac cttttcggat
    12061 ggactgcgaa ttgataccat cgcaacggat atcaaggtga cgaccattca gcctggaatt
    12121 gtcgaaacag atttttctac agtgcgtttt catggtgata aagaacgagc tgcgtccgtt
    12181 taccaaggaa tagaaacttt gcaagctcag gatattgcag atacagtagt ctatgtgact
    12241 agtcaacctc gtcgtgttca gattacagat atgaccatta tggccaatca acaggcgaca
    12301 ggtttcatgg ttcataaaaa ataaaaaatt ttctcgaaaa gttacaaatt tctgtaactt
    12361 tttttgattt cctacgaata gataagtagg aggaagaaaa tatgtataat aaagttatct
    12421 tgattggacg tttaacgtct acaccagaat tgcacaaaac caacaatgac aagtcagtag
    12481 cgcgagcaac tatcgctgtc aaccgtcgtt acaaagacca aaacggtgaa cgtgaagctg
    12541 attttgtcaa tatggttcta tggggcagac tagcagaaac tttggcaagc tacgcaacca
    12601 aaggtagtct catttccgtt gatggagaat tgcgtacccg tcgctttgag aaaaatggtc
    12661 agatgaatta tgtaaccgaa gtccttgtga caggattcca actcttggaa agtcgtgccc
    12721 aacgtgctat gcgtgaaaat aatgcaggcc aagatttggc agatttggtc ttggaagagg
    12781 aagaattgcc attttaagaa ttaaaaagtc tgagttggtc tcaggctttt tatcttgaga
    12841 aagtcagact tttttcttga ttatttctga ctaagtgata caatagaact atgaattagc
    12901 actcaggtat aaagagtgct aataatatct atctcattat ggaggaaatc agatgttgaa
    12961 accattaggg gaccgtgtgg tcttaaaagt agaagaaaaa gaacaaactg ttggaggctt
    13021 tgttctcgca ggttcagccc aagaaaaaac caaaacagcc caagttgtgg ctactggaca
    13081 aggtgttcgt accttgaacg gtgacttggt tgctccaagt gttaaaactg gagatcgtgt
    13141 cttagttgaa gcccacgcag gtcttgatgt caaagatggc gatgaaaagt acatcatcgt
    13201 aggcgaagct aacattttgg caatcattga ggaatagaag gagaaagtaa gtatgtcaaa
    13261 agaaattaaa ttttcatcag atgcccgttc agccatggtt cgtggtgtcg atatccttgc
    13321 agacactgtt aaagtaacct tgggaccaaa aggtcgcaat gtcgttcttg aaaagtcatt
    13381 cggttcaccc ttgattacca atgacggtgt gaccattgcc aaagaaatcg aattggaaga
    13441 ccattttgaa aatatgggtg ctaagttagt atcagaagta gcttctaaaa ccaatgatat
    13501 cgcaggtgac ggaactacga ctgcaacagt cttgacccaa gctatcgtcc gtgaaggaat
    13561 caaaaacgtc acagcaggtg caaatccaat cggtattcgt cgtgggattg aaacagcagt
    13621 tgccgcagca gttgaagctt tgaaaaacaa cgccatccct gttgccaata aagaagctat
    13681 cgctcaagtt gcagccgtat cttctcgttc tgaaaaagtt ggtgagtaca tctctgaagc
    13741 aatggaaaaa gttggcaaag acggtgtcat caccatcgaa gagtcacgtg gtatggaaac
    13801 agagcttgaa gtcgtagaag gaatgcagtt tgaccgtggt tacctttcac agtacatggt
    13861 gacagatagc gaaaaaatgg tggctgacct tgaaaatccg tacattttga ttacagacaa
    13921 gaaaatttcc aatatccaag aaatcttgcc acttttggaa agcattctcc aaagcaatcg
    13981 tccactcttg attattgcgg atgatgtgga tggtgaggct cttccaactc ttgttttgaa
    14041 caagattcgt ggaaccttca acgtagtagc agtcaaggca cctggttttg gtgaccgtcg
    14101 caaagccatg cttgaagata tcgccatctt aacaggcgga acagttatca cagaagacct
    14161 tggtcttgag ttgaaagatg cgacaattga agctcttggt caagcagcga gagtgaccgt
    14221 ggacaaagat agcacggtta ttgtagaagg tgcaggaaat cctgaagcga tttctcaccg
    14281 tgttgcggtt atcaagtctc aaatcgaaac tacaacttct gaatttgacc gtgaaaaatt
    14341 gcaagaacgc ttggccaaat tgtcaggtgg tgtagcggtt attaaggtcg gagccgcaac
    14401 tgaaactgag ttgaaagaaa tgaaactccg cattgaagat gccctcaacg ctactcgtgc
    14461 agctgttgaa gaaggtattg ttgcaggtgg tggaacagct cttgccaatg tgattccagc
    14521 tgttgctacc ttggaattga caggagatga agcaacagga cgtaatattg ttctccgtgc
    14581 tttggaagaa cccgttcgtc aaattgctca caatgcagga tttgaaggat ctatcgttat
    14641 cgatcgtttg aaaaatgctg agcttggtat aggatttaac gcagcaactg gcgagtgggt
    14701 taacatgatt gatcaaggta tcattgatcc agttaaagtg agtcgttcag ccctacaaaa
    14761 tgcagcatct gtagccagct tgattttgac aacagaagca gtcgtagcca ataaaccaga
    14821 accagtagcc ccagctccag caatggatcc aagcatgatg ggcgggatga tgtaagcatt
    14881 ctatagaaaa caacttataa aaaacacaaa aggagggaat gactaaccct tctttttata
    14941 atattcactt ctaaattgat ttgagctctc ctaacttata tgataaaata agactagaaa
    15001 aaggagaaga acctgatcga tgtagaagaa attctgtgca agatgccccc caatcagaag
    15061 attaattatg accgtgtcat gcagaaaatg gtagaagtat ggtagaaaaa tgagcaacgg
    15121 ccaaccattc tcgtgcatgt ttgctgtgcc ccttgtagta cctatacact agaatatttg
    15181 accaagtatg cagatgtgac catctatttt gccaattcta atatccatcc caaggtagaa
    15241 taccataagc gggtctatgt caccaagaaa tttgttagtg attttaatga gcggacagga
    15301 aatacggttc agtacctaga agctccctac gaacccaatt aataccgaaa actagttagg
    15361 ggactagagg aggagcccga aggtggcgac cgttgcaagg tttgttttga ctaccgactg
    15421 gataaaacag cgcaagtggc tatggacttg ggctttgact actttggttc agccttgacc
    15481 atcagtcctc ataagaattc tcaaaccatc aacagcatcg gaatcgatgt gcaaaaaatt
    15541 tacacgaccc actatcttcc caacgatttc aagaaaaatc aaggctacaa acgttcagta
    15601 gagatgtgtg aggagtatga tatctatcgt caatgttatt gtggctgcgt ctatgcagcc
    15661 caagcccaga atattgacct ggttcaagtt aagaaggacg ctacagcatt cttgttgggt
    15721 aaggatgttg agaaagacta ttctcatatc aaatttatag tagattgaaa ctagaatagt
    15781 acacctttac ttctaaaaca ttgttagaaa tcgat
//
LOCUS       DAVHIU010000039        15763 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVHIU010000039 DAVHIU010000000
VERSION     DAVHIU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.041"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            520..759
                     /locus_tag="VVN06_001049"
     CDS             520..759
                     /locus_tag="VVN06_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562614.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1309..3240
                     /gene="pspA"
                     /locus_tag="VVN06_001050"
     CDS             1309..3240
                     /gene="pspA"
                     /locus_tag="VVN06_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU5562615.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTVVRAEESPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEAKKKAEDAQKKYDEDQKKTEAKAEKERKASEKIAEATKEV
                     QQAYLAYLQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKKK
                     AEEAKAEEKVAKRKYDYATLKLALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVD
                     NLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQD
                     ELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAE
                     LEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAPKPEQPAPAPKP
                     EQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMATGWLQN
                     NGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMATGW
                     LQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWAKVNGSWYYLNANGAMAT
                     GWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGA
                     LAVNTTVDGYKVNANGEWV"
     gene            3641..4762
                     /gene="mnmA"
                     /locus_tag="VVN06_001051"
     CDS             3641..4762
                     /gene="mnmA"
                     /locus_tag="VVN06_001051"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5562616.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            4903..5358
                     /locus_tag="VVN06_001052"
     CDS             4903..5358
                     /locus_tag="VVN06_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5562617.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            5368..7281
                     /gene="mnmG"
                     /locus_tag="VVN06_001053"
     CDS             5368..7281
                     /gene="mnmG"
                     /locus_tag="VVN06_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU5562618.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(7488..7580)
                     /locus_tag="VVN06_001054"
                     /pseudo
     CDS             complement(7488..7580)
                     /locus_tag="VVN06_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7634..9313)
                     /locus_tag="VVN06_001055"
     CDS             complement(7634..9313)
                     /locus_tag="VVN06_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5562619.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(9315..9548)
                     /locus_tag="VVN06_001056"
     CDS             complement(9315..9548)
                     /locus_tag="VVN06_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU5562620.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            9828..10043
                     /locus_tag="VVN06_001057"
     CDS             9828..10043
                     /locus_tag="VVN06_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562621.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(10366..10518)
                     /gene="cibB"
                     /locus_tag="VVN06_001058"
     CDS             complement(10366..10518)
                     /gene="cibB"
                     /locus_tag="VVN06_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU5562622.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(10520..10705)
                     /gene="cibA"
                     /locus_tag="VVN06_001059"
     CDS             complement(10520..10705)
                     /gene="cibA"
                     /locus_tag="VVN06_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU5562623.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            10883..11566
                     /gene="tsaB"
                     /locus_tag="VVN06_001060"
     CDS             10883..11566
                     /gene="tsaB"
                     /locus_tag="VVN06_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5562624.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            11563..12000
                     /gene="rimI"
                     /locus_tag="VVN06_001061"
     CDS             11563..12000
                     /gene="rimI"
                     /locus_tag="VVN06_001061"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5562625.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            11990..13000
                     /gene="tsaD"
                     /locus_tag="VVN06_001062"
     CDS             11990..13000
                     /gene="tsaD"
                     /locus_tag="VVN06_001062"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5562626.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKTFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(13040..14346)
                     /locus_tag="VVN06_001063"
                     /pseudo
     CDS             complement(13040..14346)
                     /locus_tag="VVN06_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14622..14973)
                     /locus_tag="VVN06_001064"
                     /pseudo
     CDS             complement(14622..14973)
                     /locus_tag="VVN06_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15080..15373)
                     /locus_tag="VVN06_001065"
                     /pseudo
     CDS             complement(15080..15373)
                     /locus_tag="VVN06_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4717 a         3024 c         3615 g         4407 t
ORIGIN      
        1 tttgggagtt atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata
       61 aagaatagtt tggacttgaa ctgtatctaa aaacaggggt tggtcgatta tcagcctctt
      121 tcttgtcgtt gaaaagagtg agatatattg acttttgaaa aaaagatgtc ataattgagt
      181 tgggataaac ctaaaagtaa tatagtagat tgaatctgga atagtccgtt atgactgtta
      241 aaatatttct agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc
      301 tactatactt tctcgttaaa ccgttcggca attcatggtg acatggacac atagtagtaa
      361 gcacactatg tggcttggca gagctaaaaa ctgttccctt gcgataagcc taataagcac
      421 aacataggga gttagagaag ccgactctaa tcaaccactt tgggcagtag tgagaactgc
      481 cccgtgcttt tttattttga gaaaatatgg agtttgtcgt tgaaattact tgattgtatt
      541 ttggattatc aagaaagatt caatggaaaa acatgtcaag tatcaacgaa ttataagtat
      601 ttagagattt tcaaagtgaa tttttgcttg actgatttac atcatttatt tggcttacac
      661 aaaatcacac gagattatgc tagtcaaaca aaacctgcta ttcaagctgg tgtttttatt
      721 ttagaagatt ttagaaatat cctatgtaca atgatgtaat cgaaaggata tctttatgtg
      781 agtttatcgg cgatatcttc tatatctaag ctaacaagtt gttgcatcgt agctaaggat
      841 ttatctaaaa atactatgaa attggacgtc atattttttg aggataaaaa taaaagttcc
      901 gcagttttag gtttacgaag agacaaaagc ggagtattta aaccagttac tctacatttt
      961 acaagcgcta agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta
     1021 taaagattgt ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaataa
     1081 gctctctcat cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga
     1141 gaatcgaaag gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga
     1201 aatttgtaat aaaaatgtaa tataaaacat ttgacaaata tttacggggg gggtatactt
     1261 aatataagta taatctaaaa atgattatca gaaaagaggt aaatttagat gaataagaaa
     1321 aaaatgattt taacaagtct agccagcgtc gctatcttag gggctggttt tgttacgtct
     1381 cagcctactg ttgtaagagc agaagaatct cccgtagcca gtcagtctaa agctgagaaa
     1441 gactatgatg cagcagtgaa aaaatctgaa gctgctaaga aacattacga agaagctaaa
     1501 aagaaagcag aagacgctca gaaaaaatat gatgaggatc agaagaaaac tgaggcaaaa
     1561 gcggaaaaag aaagaaaagc ttctgaaaag atagctgagg caacaaaaga agttcaacaa
     1621 gcgtacctag cttatctaca agctagcaac gaaagtcaga gaaaagaggc agataagaag
     1681 ataaaagaag ctacgcaacg caaagatgag gcggaagctg catttgctac tattcgaaca
     1741 acaattgtag ttcctgaacc aagtgagtta gctgagacta agaaaaaagc agaagaagct
     1801 aaagcagaag aaaaagtagc taagagaaaa tatgattatg caactctaaa gctagcacta
     1861 gcgaagaaag aagtagaggc taaggaactt gaaattgaaa aacttcaata tgaaatttct
     1921 actttggaac aagaagttgc tactgctcaa catcaagtag ataatttgaa aaaacttctt
     1981 gctggtgcgg atcctgatga tggcacagaa gttatagaag ctaaattaaa aaaaggagaa
     2041 gctgagctaa acgctaaaca agctgagtta gcaaaaaaac aaacagaact tgaaaaactt
     2101 cttgacagcc ttgatcctga aggtaagact caggatgaat tagataaaga agcagaagaa
     2161 gctgagttgg ataaaaaagc tgatgaactt caaaataaag ttgctgattt agaaaaagaa
     2221 attagtaacc ttgaaatatt acttggaggg gctgattctg aagatgatac tgctgctctt
     2281 caaaataaat tagctactaa aaaagctgaa ttggaaaaaa ctcaaaaaga attagatgca
     2341 gctcttaatg agttaggccc tgatggagat gaagaagaaa ctccagcgcc ggctcctcaa
     2401 ccagagcaac cagctcctgc accaaaacca gagcaaccag ctccagctcc aaaaccagag
     2461 caaccagctc cagctccaaa accagagcaa ccagctccag ctccaaaacc agagcaacca
     2521 gctaagccgg agaaaccagc tgaagagcct actcaaccag aaaaaccagc cactccaaaa
     2581 acaggctgga aacaagaaaa cggtatgtgg tatttctaca atactgatgg ttcaatggca
     2641 acaggttggc tccaaaacaa cggttcatgg tactacctaa acgctaacgg ttcaatggca
     2701 acaggttggg tgaaagatgg agatacctgg tactatcttg aagcatcagg tgctatgaaa
     2761 gcaagccaat ggttcaaagt atcagataaa tggtactatg tcaacagcaa tggcgctatg
     2821 gcgacaggct ggctccaata caatggctca tggtactacc tcaacgctaa tggtgatatg
     2881 gcgacaggct ggctccaata caacggttca tggtattacc tcaacgctaa tggtgatatg
     2941 gcgacaggct gggctaaagt caacggttca tggtactacc taaacgctaa cggtgctatg
     3001 gctacaggtt gggctaaagt caacggttca tggtattacc tcaacgctaa cggttcaatg
     3061 gcaacaggtt gggtgaaaga tggagatacc tggtactatc ttgaagcatc aggtgctatg
     3121 aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg cttaggtgcc
     3181 cttgcagtca acacaactgt agatggctat aaagtcaatg ccaatggtga atgggtttaa
     3241 gccgattaaa ttaaatcatg ttaagaacat ttgacatttt aattttgaaa caaagataag
     3301 gttcgattga atagatttat gttcgtattc tttaggtacc tatcttatga tttcaggaaa
     3361 tgtcattaaa aaaacgactc cttttctcta acttgaaaaa tagattagag aaaatgggtt
     3421 gttttattta ttatagttat ttgaatgaag ataagaagaa ggtatactca catcattcac
     3481 ataatctgta tattgactat aagttttaaa aaacaatttt taagctcttc cttgtcttct
     3541 ctaaccaagc gtgttataat gaatactgct caagcgacct tcaatcgtga agcacacacg
     3601 accttcaatc gtgaataaac gaatagatgg gagacttacc atgagtgata actctaaaac
     3661 acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg gtgacggctc ttttgctcaa
     3721 ggagcagggc tacgatgtga tcggtatctt catgaagaac tgggatgaca cagatgaaaa
     3781 cggcgtctgt acggcgaccg aagattacaa ggatgtggtt gcggtggcag accagattgg
     3841 cattccctac tactctgtca attttgaaaa agagtactgg gaccgcgttt ttgagtattt
     3901 cctagcggaa taccgtgcag ggcgcacgcc aaatccggac gttatgtgca acaaggaaat
     3961 caagttcaag gcctttttgg actatgccat aaccttgggg gcagactatg tagcgactgg
     4021 gcattatgct cgagtggcgc gtgatgagga tggtaccgtt cacatgcttc gtggcgtgga
     4081 caatggcaag gatcagacct atttcctcag ccaactttcg caagaacaac ttcaaaaaac
     4141 catgttccca ctaggacatt tggaaaagcc tgaagtacgc agactagcag aagaagcagg
     4201 cctttcgact gctaagaaga aagactcgac agggatttgc tttatcggag aaaagaactt
     4261 taaaaacttt ctcagcaact acctgccagc tcagcctggt cgcatgatga ctgtggatgg
     4321 tcgcgatatg ggcgagcatg caggtcttat gtactataca atcggtcagc gtggcggact
     4381 cggtatcggt gggcaacacg gcggtgacaa tgccccttgg ttcgttgtcg gaaaagatct
     4441 aagcaagaat attctctatg taggacaagg attctaccat gattcgctca tgtcaactag
     4501 cctagaagcc agtcaagtcc actttactcg tgaaatgcca gaagagttta cgctagaatg
     4561 tacggctaaa ttccgttacc gtcagcctga ctctaaggtg accgttcatg tcaaaggaga
     4621 aaagacagag gtcatctttg cggaaccaca acgcgcgatt acaccaggac aggcagttgt
     4681 cttttacgat ggcgaagagt gtctaggtgg cggtttgatt gacaatgctt accgcgatgg
     4741 acaagtttgt cagtacattt agattgacaa attttctcaa tttgctacaa taataacagc
     4801 aatagaaatg atggtcaaag ctcatggatg ttgcaggctt ttttgtcctg cacttctttg
     4861 tagttttgac tgtttttgtg tcgtttaagg gaaaggacaa gaatgactca acaagacttt
     4921 cggacaaaag tagacaatac ggtttttggt gttcgggcta cagccttgat tgtccaaaat
     4981 cacaagcttc tagttaccaa agacaagggc aagtattaca ctattggagg tgcgattcaa
     5041 gtcaatgaaa gcacggaaga cgcggtagtc cgtgaagtga aggaagaact gggtgtcaaa
     5101 gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt ttgaagtgga cggcgtttcc
     5161 tatcacaata tcgagtttca ttatctggtg gatttgcttg aagatgcccc gttgaccatg
     5221 caggaagatg agaaaaggca gccctgtgag tggattgact tggataagct ccagaatatc
     5281 cagctagttc cagccttttt aaaaacagcc ctaccagatt gggaaggcca actaagacac
     5341 attcatcttg aggaatagga gagaaacatg acttatcatt ttactgaaga atacgatatt
     5401 attgtaattg gtgcgggaca cgctggggtt gaggcttcct tggccgctag ccgtatgggc
     5461 tgtaaggtcc tgcttgcgac catcaatatt gaaatgctgg ctttcatgcc ttgtaatccc
     5521 tctatcggtg gttctgccaa ggggattgtc gtgcgtgaag tcgatgccct cggtggcgag
     5581 atggccaaaa ccattgacaa gacttacatc cagatgaaga tgctaaacac agggaagggg
     5641 ccagctgtcc gtgcccttcg tgcgcaggct gacaaggaac tttactctaa ggagatgcgc
     5701 aagacggttg aaaaccaaga aaatctgacc cttcgtcaaa ccatgattga tgagattttg
     5761 gtggaagatg gcaaggttgt cggtgtgcgt acagccaccc atcaagaata tgctgctaag
     5821 gctgttattg tgacgacagg gactgctctc cgtggggaaa ttatcatcgg agacctcaag
     5881 tactcatcag gtcctaacca cagcttggct tctattaacc tagctgacaa tctcaaggaa
     5941 ctgggtctcg aaatcggtcg tttcaagaca ggaacccctc cacgtgtcaa ggcttcttct
     6001 atcaattacg atgtgacaga aattcagcca ggagacgaag tgcctaatca tttctcatac
     6061 acttcacgtg atgaggatta tgtcaaggac caagtaccat gctggttgac ctataccaat
     6121 ggtaccagtc atgagattat ccaaaacaac ctccaccgtg cgcctatgtt tacaggtgtg
     6181 gtcaagggag tggggcctcg ttactgtccg tcgattgaag acaagattgt gcgctttgcg
     6241 gacaaggaac gtcaccaact cttccttgag ccagaagggc gcaatactga ggaagtctat
     6301 gtgcaaggac tttcaaccag tctgcctgag gatgtccagc gtgacttggt gcattccatc
     6361 aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg ctattgagta tgatatggtc
     6421 ttgcctcatc agttgcgtgc gactttggaa accaagaaaa tctcaggtct cttcactgct
     6481 ggtcagacaa atggaacatc aggttacgaa gaggcagcag gccaagggat tatcgcgggt
     6541 atcaatgcgg ctctgaaaat ccaaggcaag cctgaattga ttttgaagcg cagtgatggt
     6601 tatatcgggg tgatgatcga cgacttggtg accaagggaa ccattgaacc ctaccgtctc
     6661 ttgaccagtc gtgctgaata ccgtctcatt cttcgtcatg acaatgctga tatgcgcttg
     6721 actgagatgg gacgcgagat tggccttgtg gacgatgaac gctgggctcg ttttgaaatc
     6781 aagaaaaatc aatttgataa tgagatgaag cgcctagaca gtatcaaact caagccagtc
     6841 aaggaaacca atgccaaggt tgaggagatg ggcttcaaac ccttgaccga tgcagtgaca
     6901 gccaaggaat tccttcgccg tccagaagtt tcttaccaag atgtggtggc cttcatcgga
     6961 ccagctgcag aagacttgga tgacaagatt atcgaattga ttgaaacaga aatcaagtat
     7021 gaaggctata tttccaaagc catggaccag gttgccaaga tgaaacgcat ggaagaaaaa
     7081 cgcattccgg ccaatatcga ctgggatgac attgattcta tcgcaaccga agcccgtcag
     7141 aagttcaaac tcatcaatcc agaaaccatc ggccaagcca gccgtatttc gggagtaaac
     7201 ccagcagata tttctatttt gatggtgtat ctggaaggta aaaatcgtag tatttctaaa
     7261 actcttcaaa aatcaaaatg atacgtcgtc ggcttcttac gaatgagttc aaagcttggc
     7321 tttgattcat ctccagcctc ccatagtttc ccgaactatg ggagctaact caagttatgc
     7381 tttcgcttag ccttctagtc tgatttcgat ttttattgat actcttcgaa aatctcttca
     7441 aaccgcgtca acgtcgcctt gccgtaggta tggctactga cttcgtcagt tctatctaca
     7501 acctcaaagc agtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc
     7561 attgagtatg agttttaatc tcaaaagaaa gcatagagaa aaacagctcc gaggacgggg
     7621 ctgttttctt gctttattct tcatctggtg tgaggatcat cgggatgatg atcggttcac
     7681 gttcggtatt ttcatagagg aaggggcgaa tagcgttgac aatggcacca ttgacagatt
     7741 gcacgctagc atccttattt ttcagtgcga tacgaatggc attgaagagg atacgctggc
     7801 tttggcgaat caagtctcca gactctctca tgtagacaaa gcctcggctg aggatatctg
     7861 ggccagacag aatcatctgc gatttgaagt caacagttgc gactgctaga acgacaccgt
     7921 cttcagatag atcgcgacga tctttgagga cagctgcgcc aatttcaccg atacgatttc
     7981 catcgacata gatatcttgg gcgttgaaat gacctgcgat acgagctgag tcagcagtaa
     8041 gggcaagcac atcgccattg ctcatgataa agatattgtc cttctcaaca ccagtatcca
     8101 ctgctagtcc agcgtggact ttttgcatgc ggtattcacc gtggacaggc atgaagtatt
     8161 ttggcttaat caagcggagc atgagttttt gctcttgctg gccaccgtgt ccagatgtat
     8221 ggatattgtt cactttaccg tggataactt cgacaccagc ttcagaaatg atgttaatca
     8281 gcttgttgac gctagtagtg tttccaggga tgggactaga agagaagata acggtatcac
     8341 ctggttgtaa ttgtacttga cggtgggttc cgttggcgat acgagagagg gctgccatag
     8401 gctcaccctg actacctgta cagaggataa gaacttctcc tgcaggataa tctttgattt
     8461 catttggctc gataaaggtt cccttaggag ctttgatgta gccaagatcg attccgttga
     8521 caatggcctt ttccatagaa cgaccaaaga ccgcaatctt gcgtccagtc ttaacagcag
     8581 cttctgttgc ctgctggaga cggaagatat ttgaggcaaa ggatgcaaag atgatacgtc
     8641 cttcaatacc ttggataatc ttcataatgg actgaccaac gactttttca gagttggtaa
     8701 aggttggtac ttccgcattt gtcgagtcag acaggagaca gagcacgcct tcttcaccaa
     8761 gcgcagccat acgatgcaag tccgcaggtt ctccaactgg agtaaagtcg aacttaaagt
     8821 cacccgtaca gacgattttc ccttgaggag tatgaatgac aatccccaaa ggctctggaa
     8881 tagagtgagt cgttctaaag aaagttgcct tgagattttt aaaggtcaac tcggtgttgt
     8941 ggttgatttc gtaaagtttg gcgttgcgca agaggccgtg ttcttcgagt ttcccacgga
     9001 tcaaagccaa ggcaagcggt ccagcgtaga tagggacatt tgcttgcttg agtaggaacg
     9061 gaatcccacc aatgtggtcc tcgtgtccgt gtgtgattaa aacagccttg acgcggtcga
     9121 tattgtccac gatgtaagag tagtcaggaa tgacatagtc gataccaagc aagtcatctt
     9181 ctgggaattt aatcccagca tcgacgatga taatctcgtc ttggtattca attccgtaag
     9241 tgtttttccc gatttctcct agaccaccga tggcaaaaac gccgacttct tcaggtttaa
     9301 gagtgtaggc catattagaa ctccgtaatt tcgaaggcgc cagtttcttt ttcgtaatcg
     9361 agcaatttgt cagacaagag ttcgatatac tcgatattgt actctgggcg attttcttcg
     9421 acaagttggc gagcagtgat acggccctca agttctgagc tggcatcgat gtctaggtaa
     9481 agcgtgcgtg ttgtttcacg gcgtgggcta cgttcttttg tttcttgata aaaaactttg
     9541 taaatcatat agttcctttc tatttacagg tcagcttatt ccaaactttt agcagatatt
     9601 tgcttgattt cattccattt tgtgtctaga ttgaccttga ttcgttacaa ttatttcaat
     9661 tataccacaa aatgaaaaat tagtaaaaga cggaaacgag aaagttaggg gacgatttct
     9721 ttggtaagcg tttgcaagaa taaagaaaat gtattatgtt gataacggaa gtgaaattta
     9781 ttgcttgtca taattccata tagattttaa gtcaaggagg ttgccctatg tataacttat
     9841 ttgcttttgc tatcggagct cgcttggtag cttttattgt ttttgttgct tgcgtttatg
     9901 cggggaatct tctatttgcc aaactggaac gacgacaaac caagttcttg tggaaaatga
     9961 cctttgtgac tctctactcg ctttttctcc tcctcgtgac ctatgtcgtt tggtttgtat
    10021 tttactttga attaaatgct tagatgccct gcttatgctg gggttttttg tttggggatg
    10081 tagagatagt agatttacga cacgaaaatt ccaccccatc tatcaagatg aggtggtctt
    10141 tgcatttttc taacatgaca tgacaatgga attttcaaag cttattattt ttatgggagt
    10201 cattgttctg atcaaaaata gagttgaagc ataaaaataa aacaatacaa acagataaat
    10261 cgaatggtaa agggatggta gtaatgttca aatcattgag aatgctgagc aaactgtaag
    10321 agatgaagac aataaaattt atccttctaa aaatgtgttt cttatctagc atgacttacc
    10381 aaacttttta cgaaggtctt ttccaatggt ataacctgcc ataaataaag cgataccaac
    10441 accgacacca aagccaccct tgatttcttg caattcctta ttagaaattt cacgataatt
    10501 gttcaaatct ttcatcatat taatctcctc ctaaaaaata accagctgcc aaaccagaac
    10561 ctataagtgc tgttccacct gcaatagcct tccaagatac tgctactcca aagataacca
    10621 agggagcgag accgccatca atatctgata attcattttc agataaggat aaaaattgat
    10681 tgtcaagaat gtcaaaattt gtcattgaac tttcctcctt ttttcttgtt cacttttata
    10741 ttcggaaaag aaaaggaaaa gtgagcattt ttttaaatta tttttagtta agtgagggca
    10801 ctcttgtctg ggggcaaaga aaattcccat gtgccagctt ttgtagtata atagtaagca
    10861 gacaaaaaga taggaatgtg ttatgaaagt attagctttt gatacgtcca gcaaggctct
    10921 ttctctggct attttagagg ataagcaggt tcttgccgag acgacgatta atattaagaa
    10981 aaatcacagt attactctta tgcctgccat cgattttttg atggcaagtt tggattggac
    11041 acccaaggat ttggaccgaa tcgtggtagc tgaagggccg ggtagctata caggcttgcg
    11101 aattgcggta gcaactgcta agaccttagc ttacaccctg aacattgagt tagttgggat
    11161 gtcgagtctc ttggctctgg tgccccacca acaagaaggt ttgtttgtcc ccttgatgga
    11221 tgcgcgtcgc aataatgttt atgcaggatt ttatgaaaat gccaaacctg tcatggcaga
    11281 agcgcaccta tcttttgaag aggtgctaga aaaagtcaag ggtactagtc aggtaacctt
    11341 tgtcggagaa gttggcccct ttgttgagca gattcaaaaa cacttgccaa ggactgatta
    11401 caaagaaaca ttgcccaatg cagctaatct agctcttttg gcctgggaca aggaagcaga
    11461 ctccttgcat gattttgtgc cgaattacct caaacgagtc gaggctgagg aaaactggct
    11521 caagaaccat accgagtctg gcgagtctta cattaaacgc ctatgataga aatcaagcga
    11581 attcaacaac agcctgacct agctcaagcc atctacgctg ttatggcagc tgtttaccta
    11641 gtcagtcctt ggactctgga gcaaatccaa gcagatctgt cccaagacca gacttggtat
    11701 gcattggctt atgatggggc agaagtgatt ggatttctag ctgtgcagga gaatcttttt
    11761 gaagcagaag tcctgcaaat cgctgtcaaa ggagcttatc agggtcaggg aattgcgtca
    11821 gccttgtttg ctcaattgcc gacagacaag gaaattttcc tcgaagtcag acagtcaaat
    11881 caacgagcgc aagcatttta caagaaagaa aagatggcag ttatcgctga gcgaaaggcc
    11941 tactaccatg acccagtcga ggacgccatt atcatgaaga gagaaataga tgaaggatag
    12001 atatatttta gcatttgaga catcctgtga tgagaccagt gtcgccgtct tgaaaaacga
    12061 cgatgagctc ttgtccaatg tcattgctag tcaaattgag agtcacaaac gttttggtgg
    12121 cgtagtgccc gaagtagcca gtcgtcacca tgtcgaggtc attacagcct gtatcgagga
    12181 ggcattggca gaagcaggga ttaccgaaga ggacgtgaca gctgttgcgg ttacctatgg
    12241 accgggctta gtcggagcct tgctagttgg cttgtcagct gccaagacct ttgcttgggc
    12301 tcacggactt ccgctgatcc ctgttaatca catggctggg cacctcatgg ccgctcagag
    12361 tgtggagcct ttggagtttc ctttgttggc actcttggtc agcggtggcc acacagagtt
    12421 ggtctatgtt tcggaggcag gagattataa gattgttggg gagacacgag acgatgcagt
    12481 tggggaggct tatgacaagg tcggccgtgt catgggcttg acctatcctg caggtcgtga
    12541 gattgacgag ctggctcatc aggggcagga tatttatgat ttcccccgtg ccatgattaa
    12601 ggaagataat ctggagttct ccttctcagg tttgaaatct gcctttatca atctttatca
    12661 caatgccgag caaaagggag aaagcctgtc tacagaagat ttgtgtgctt ccttccaagc
    12721 agcagttatg gacattctca tggcaaaaac caagaaggct ttggagaaat atcctgttaa
    12781 aaccctggtt gtggcaggtg gtgtggcagc caataaaggt ctcagagaac gcctagcagc
    12841 cgaaatcaca gatgtcaagg ttatcatccc ccctctgcga ctctgcggag acaatgcagg
    12901 tatgattgcc tatgccagcg tcagcgagtg gaacaaagaa aacttcgcag gctgggacct
    12961 caatgccaaa ccaagtcttg cctttgatac catggaataa agagtggctc tttgtcaagt
    13021 gtagtgggta ggcgaaaagc tacaatctgg agagtacgaa attcgtactc tcttttttga
    13081 tgttcaaagt catcaaaatc cgttttttaa aattgttaaa gttccggaaa ccaaaggctt
    13141 tgcgcttgat aatcttaatc aaattattgg tcgcttcaag tttagcgttg gaataaggca
    13201 gcttaagtgc gttgataatc ttatctctat cccttaaaaa agtccaaaag accgtttgaa
    13261 aaagcggatg aaccgttggc agttcttgct caatcaatcc aaagaaatgg tcggcattct
    13321 tctcttgaaa atgaaagagc aggagttgat agagttcgta gtgaacctgt aatccctcag
    13381 agtaagaaag cagcttatct cgtacttccc gatgggttaa gtgcattcga aacgtgggct
    13441 gatagaatcg tttctgacag agcttgaagc tatactggtg tagtaaatca gtccagtgga
    13501 ctgatttagc ccgagcctag aaacgaaaaa gcgagggttc caaaagcgct tgagcgcccg
    13561 atactccaag gattttcggt caaattggtt catgatttgg attcgtactc ggttcatagc
    13621 tcggctcaga tgttggacaa tgtggaagcg gtcaagaaca atcttcgcct ttggaaagag
    13681 ttgcttagcg agccgataat aagggctgta catgtccatg gtgatgacct cgaccagctc
    13741 ccgaacctct ctctgatagc gttggaaatg gtttcgaatc accgcatgag ttcgcccgtc
    13801 taaaattgcg aggatggatt tggactcaaa atcttgggca atgaagctca ttttgctctt
    13861 tttgaagcta tactcatccc aactcataac ttttggcaac ttggtccaat cggtttcaaa
    13921 cttaaattcc ctcagttttc ggatgacggt ggaagttgag accgccaatc tgtgggcaat
    13981 ctcagtcata gactgctttt caaggaggag ctgagcgatt ttttgtctca ccatgttgga
    14041 aatctggcaa tttttcttga caatggatgt ttgagaaacg accaccttaa ggcaattctt
    14101 gcactgaaag cggcgctttt tgagatgcag taccgtgggt aaaccctgac agtcgagaag
    14161 cggaatttta gaggctttct ggaagtcgta tttgatcatc ttcccttgac aatgaggaca
    14221 aggaggagcg ggggaatcca actttgcctg aacgactaga tgggtttggt atttcagaac
    14281 agacaagatt ttgatatttt tgtcttccaa tccgagcaaa tctgtggtat tcttaagttg
    14341 ttccatatga accctttcta atgagttggt tagacgcttt tcattatagg tcatatgggg
    14401 ctttttttct acaagaaacg accctataat tcctggggtg ggattaccca ctacagaaat
    14461 tatagagcca aagcattcca aagtcttgtc tgactctgga atgttttctg ttcacagttg
    14521 ttttgtaact gcctaataag taaccgcgct ataacgaggt atctaccgag attgtcaatt
    14581 ctccagtata ctgttataaa aaacaaaact ggaggatttt tttatgtccc cccatcgtcc
    14641 catcgactgt tccgtagctg cccaataacg gagtatagtc ggctgaaaca agaataggac
    14701 aaaagtgcct caagaaaagc atcgtaattt gacaatatta ttctgagatg tttttgatgt
    14761 aagcccatat tttctcaatg ggattatact cgggtgaata ggtaggaagt ggtaacagtc
    14821 tatgccctgc tccttacaca tgttcattct gtgaaacctt gcatggtcca taatgataag
    14881 ggatggtgta tctaaagtag gtagtaagaa tttttggaag caagcttcga aaaagtcact
    14941 cgtcatcgta tctttatagg tcatcggagc catagtatat tgaaacaaaa aagggcatta
    15001 gtacttctat aacagtatcg caatctctgg tgtgcgttgt ctcgatctta attcaaataa
    15061 ttatatcttc ttccagagag actaactgcc tgctcaaggc tcgatcatat tcgagataaa
    15121 aataggtctc aacccctgtc tcataaatat aaataggagt caggtagctt aagtgattca
    15181 attctttaag gaaccgattt actttttctg ggtcttgttc gcagtaggta cggctctttt
    15241 ttagactata tcccatagcc tttagagcgt aatgaatagt tgttggagga cagttgaatt
    15301 cagcagctat ttcagtcaaa taagcatcgg gatgagtttc aatatagatc tttaatttat
    15361 ctctatcaac tttttcaatt aatagtggaa gttttcttaa agttagtgtg ttgaaacgtt
    15421 tttatacagt gctaaaattt gaagtgtagt gcagatgtat ttgtaatagt attatagcaa
    15481 ttctttggag aaggtcgaga caagagaaaa taggtatctt tgagatataa gcatgattta
    15541 ctataaaatt attgagttat tcataaaaat ttctctatca aatatcataa tgcgtcgttt
    15601 aaggagatgg ttacaatgca atatagttgt ggaaaaataa atattaatat ccctgatgga
    15661 tatggagaca ttaaggatat agtgttttcg gctcatatta tagttaggta taataatgga
    15721 cattgtggag gtatagatcc gcatataatt ggactttgta aaa
//
LOCUS       DAVHIU010000040        15127 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVHIU010000040 DAVHIU010000000
VERSION     DAVHIU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.042"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(220..1827)
                     /locus_tag="VVN06_001066"
     CDS             complement(220..1827)
                     /locus_tag="VVN06_001066"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5562627.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2217..2804)
                     /locus_tag="VVN06_001067"
     CDS             complement(2217..2804)
                     /locus_tag="VVN06_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU5562628.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2932..3381)
                     /locus_tag="VVN06_001068"
     CDS             complement(2932..3381)
                     /locus_tag="VVN06_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562629.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3386..3775)
                     /locus_tag="VVN06_001069"
     CDS             complement(3386..3775)
                     /locus_tag="VVN06_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU5562630.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRGGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3802..4428)
                     /locus_tag="VVN06_001070"
     CDS             complement(3802..4428)
                     /locus_tag="VVN06_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5562631.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4442..5005)
                     /locus_tag="VVN06_001071"
     CDS             complement(4442..5005)
                     /locus_tag="VVN06_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5562632.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5170..5404)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5458..5961)
                     /locus_tag="VVN06_001072"
     CDS             complement(5458..5961)
                     /locus_tag="VVN06_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU5562633.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6154..6984)
                     /locus_tag="VVN06_001073"
     CDS             complement(6154..6984)
                     /locus_tag="VVN06_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5562634.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6969..8651)
                     /locus_tag="VVN06_001074"
     CDS             complement(6969..8651)
                     /locus_tag="VVN06_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562635.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQAAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8753..9010)
                     /locus_tag="VVN06_001075"
     CDS             complement(8753..9010)
                     /locus_tag="VVN06_001075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562636.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9017..9565)
                     /locus_tag="VVN06_001076"
     CDS             complement(9017..9565)
                     /locus_tag="VVN06_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU5562637.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9580..10428)
                     /locus_tag="VVN06_001077"
     CDS             complement(9580..10428)
                     /locus_tag="VVN06_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU5562638.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10616..11965
                     /gene="trkA"
                     /locus_tag="VVN06_001078"
     CDS             10616..11965
                     /gene="trkA"
                     /locus_tag="VVN06_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU5562639.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11969..13408
                     /locus_tag="VVN06_001079"
     CDS             11969..13408
                     /locus_tag="VVN06_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU5562640.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13534..>15127)
                     /locus_tag="VVN06_001080"
     CDS             complement(13534..>15127)
                     /locus_tag="VVN06_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5562641.1"
                     /translation="GFIATMPLIMFSSFLMIIIMIPKNFGVELPSPAIVWMRKVYMLT
                     MGVLGVIVSGTVAKSLAGNVNRKMPPGKVINDISVMLAAICSYLVLTVTLVVDEKTGS
                     TSLSTNYLGSQGLVTSFVSAFITVNVYRFCIKRDITIHLPKEVPGAISQAFRDIFPFS
                     FVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGVESYPAMMLIWFMCALLWFVGIH
                     GPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFVVPFIL
                     IFFMRSKQLKSVGKATITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLGKVF
                     IDFFGMNGFYIQLPWAFPGPLGLLIGTNFQSISFVFLSLMLVVDILIYLPFCRAYDRQ
                     LLMKEDVVSSNDIILEEETSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGA
                     QLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGME
                     YIHLTKSPSKALQFVLEHYQAV"
BASE COUNT         4562 a         3429 c         2758 g         4378 t
ORIGIN      
        1 aaaactttgc tagaaattga tttggctttc cgaattaatt tgttcagatt ttatttcatt
       61 tcaatatact gataaatcca ttcactagca cagggcaaga gggagtcgct attaatttca
      121 acggtagcag gtacatacag aggactccta cctcagatat actgcaatat cattgtatgc
      181 aaaaaacctc tgagattttt ctcaaaggtt ctgattttgc taattgctgt tctcaaccgc
      241 tgcagtaaca aaggcagtgt agagttcttc tggtcggttt ggacggcttg acagttcagg
      301 gtgatactga caagctacaa agaatttatt ttcaggaatt tccacgattt ctaccaaacg
      361 attgtctgga gaaactcctg aaaagacaaa acctgctgcc tcaaactgct cacggaaggc
      421 attattaaac tcataacggt gacggtgacg gcgttgcacc acttcttgat tgtgataagc
      481 agcagcagcc ttagagccac gtttcaactt agacggataa agtcccaaac gaagggttcc
      541 acccatatcc tcaatatcaa tctgatcacg catgatatca atgatagggt attttgtttc
      601 tggtgcaagc tctgcagaat tggcaccttc aagacctaaa acgtgacgag caaactcgat
      661 acatgtcaac tgcattccca agcagactcc caacattgga acatcatttt cacgcgcata
      721 gcggatggct tggattttcc cttctgtacc acgttgacca aaaccacctg gtacgatgat
      781 cccgtccgca tcagacaaga gttctgctac attctctgct gtcacatcat tggcattgac
      841 ccaattgatt ttaacttcta catcattgac atagccagag tgtttcaagg cttcgaccac
      901 tgagatatag gcatcttgca actccacata cttaccaaca agggaaatct taacttgttt
      961 cttgaggttc atgaccttgt ccaccatggc tgaccattct gtcatatccg ctgctggtgc
     1021 gtctaatttc aaatgatcac aaacaatttg gtccatccct tgtgcctgca agttcagtgg
     1081 aatttggtaa aggtgttcaa catccaacga ttcgataacg gcttctggtg ccacatcaca
     1141 gaactgggcc agtttatttt taattccttg accagctggc tcttctgtac gaataaccaa
     1201 catatttggt tggattccca agccacgcaa ttctttgaca gagtgttggg ttggtttggt
     1261 tttcatttca ccagcagcct tgaggtaagg aagcaaggtt gtatggatat acatgacatt
     1321 atccgcaccc acatctgcct tcatctgacg aagagcctct aggaatggca aggactcgat
     1381 atctcctact gttccaccaa cctctgtgat aatgacatca gagtcggtcg ttagagcggc
     1441 acgcttgatt ttttctttca aagcatctgt gatatgagga atgacttgaa cagttgcccc
     1501 aaggtattct ccacggcgtt ctttacgaag aacttcactg taaattttcc cagttgtcac
     1561 gttggaatat ttgttgagat tgatatcgat gaaacgttca tagtgaccca agtccaaatc
     1621 tgtctcagct ccgtcatctg tcacaaaaac ttccccgtgc tggtaaggac tcatggttcc
     1681 cggatcaata ttgatataag ggtcaaactt ttgaatggtt actttgagac cacgattttt
     1741 caagagacgg cctagactcg ctgccacaat ccctttccca atagacgata ccacaccacc
     1801 agttacaaaa atatatttcg tagacataga ttcctctttc taaaatgctc aaggtcttgt
     1861 taactacgag gggatataga aaacaagacc ctactaataa gaataatctg ggacgactgc
     1921 accagattca cagctaatac tcttcgaaaa tctcttcaaa ccgcgtcaac gtcgccttgc
     1981 cgtagatatg tgttcctgac tttgtcagtc ttatctacaa cctcaaaata gtgttttgag
     2041 cagcctacgg ctagtttcct agcttgctct ttgattttca ttgagtataa aatacaagaa
     2101 gataacttct tgaaaaaaca aaaatagctc cctaagaact agggagcccc gacctctaaa
     2161 agaggtgccc aaacaatatg atacctaaaa ataggataat tgtcaatagc taactcttat
     2221 tcctcgctgg tttcagcatc gtcatctgaa aactcgtcgt cgtcttcgtt gagatccaca
     2281 tcttctccca agtcatctgg agcgatttcg ttgatttctg catcataagc ttccacttca
     2341 tttttttcat catctggatt ttcatcatcg tatgaaagag ctggatctgc ttcgtatgca
     2401 tcttcgtctt ctggatcatc tgcattgtag tcaatggcat ctgaatcacc atccataaag
     2461 gcattgacac gttttttctt agcttttggt gctacttcat cgtcgtcatt ttcttcaaga
     2521 gcgatgattt cttcgtcgat ttcgtccaca ccataccatg aacgaagacc ccatttgttg
     2581 tccccaagtg agatgaagct accgtcaaag ttcaactctg tgtagaacaa aggcaaagct
     2641 tcgcggatat cgctgtttga tgttccaagg tagttttgaa tttcgtttac aagatcgcta
     2701 aaatgcatct catgatcgcg accacgaagt tccaagatag cacgcgctac ctcaatcata
     2761 gatagttcac ttttttcttg cccagcaaat acttctaatt ccaaagcgtt tctcctcatt
     2821 tatactacta tcgccagagc gaacagactc tgacctcatt ttatcattta ctctttattt
     2881 tacgataatt ttgcggaata gtcaaaggtt aagggggaga aagtggcagg attagactaa
     2941 ttccaatata aaactccttc ctttttctgt tgctccattt tccacaaatc caagcgactt
     3001 gaaacacctc ctagaagcat gattgtaggt gtagatttcc ttgactctca attctttcca
     3061 tccttttact cgagccaatt caatcaaagc acttagaatc ttttttccaa gtcctcgatg
     3121 ttggtaagcg gaattcccaa tcacaatggg gagattatcc tgagatagtg taatatcccc
     3181 aattggaaac cattctccct tctccttgac ttcaatccaa aaaagctcac catgccgatc
     3241 caaataggaa tacatggctt ccaaggtcgc ttgactgtaa ggaagcttca ccccatctac
     3301 gaggtaaacc aagttcacat cctgatacca agcaaaagct tcctcacaat aattgacctt
     3361 ataataagga acaaaacaca atgcactacc ttttcaacaa aagagttgct gcttgattaa
     3421 aaccatcaca ccagttatac cattttgctt catacccatc ttgagctagg atacgatctt
     3481 ctaaatcaaa aacagagtaa atctttcttt cctcgcaagc ttgcgcatag agatgatata
     3541 gttcatcacc accacctcta tcccactcag cagaaatcgt atcccgacct gccaataaag
     3601 cctgataagc cctgtgatgc ccatctgtaa tcagcaaaca atctccaaag gcaagaatac
     3661 tgattggatc gacttggatt gtttctgccg actggtaaag catctgaata tcttgcaact
     3721 tcttttctga taaatatagt tgagtcggat gaagatctgc tatattgact ttcatttctt
     3781 tctctcaagg gaattcgata ctcacttctg tttgccttta aatcgccatt ggaagcggag
     3841 cttgtcataa aagggaaact cgataaacag gactcccaag cccacacaga gactggcaag
     3901 gacgtctgat gggtaatgaa ctcccagata gactcttgat accagcacac tgactaggta
     3961 gaggccaagg acgatttgta cgatttttct ccagacctga tctttaatcc gctgactaag
     4021 aataacaatc aaagtcccta ccatcagcgt tacagctaga gaatgcccac ttgggaagga
     4081 aaatcccttc tcctccacca gatgtaaaat agctggtcgt gggcgctggt agatattttt
     4141 aaaggtcacg attaaaagac ctgccaaagc cagatttccc agcatgaaga aactttctat
     4201 cttccatcgc ttacgataaa agacaaaagc tataatgaca acccaagtga taatcactgg
     4261 aatatcaatc agacgtgtga gggatcgaaa aagaatagtc aaataatctg gtaagtctcc
     4321 tcgaatggca gtctgaatcg attggtcaaa accgaccagc gtttcagggt aaaatttgac
     4381 catgtagcca agaataacga aaagtaaaag ggcaaaactg cccttcatta aaaatgtttg
     4441 tttatctctc ataatgtttt aaggttggtt tcaagagaac atacaacaac cagaatgaaa
     4501 cggaaaagat tacaccctca atcaagttaa aaggtaatac catggtcatt aggtagttgg
     4561 aaagtcccaa aatttttcca atatcaaagt tagcaaactt agcgtacaaa ggaacagcat
     4621 aaacatagtt gagaaccaac atggccaagg ttaaaccaac agttccagct agagagccta
     4681 gtaggaaacg aagggttgtc cgttcctttt tccaaatcaa agcaaatacg atgacaaaaa
     4741 ctcccaaagc tacgatattc atcggcaaac caatgtaagt attcactcct tggctgttaa
     4801 gaagcaattt caatagtgag cgaagcaaga gcactcctag agcagcaggc aaatccatga
     4861 ccaccagacc aacaaggact ggcaagatac taaattcgat cttgaggaaa gatgccgctg
     4921 gtaaaagcgg aaagtcaaag tacatcagca caaatgagat ggctgataga attgcaatgg
     4981 tcgaaagtcg acgtgtgttt gtcataacag gttcctccaa ttttctataa aatcagaaga
     5041 agttggaaag gattcctcta tctattctca ctttttatat cccaaaagtt ccctcttact
     5101 ctattaaaga aaaacaaagc aagtggttac aatccggcta taaatctatc aaaacagaca
     5161 aagctattct ttcgtcttct cccatccaga ctatactgtc ggttgtggaa tctcaccaca
     5221 tcagcttgcg ctcgcggact tctttaaaga gaaggaaata gagatttttc ttttagacta
     5281 ggcgacaagc tgtaggcata acttctggtt aggcaaagct tgtcaacaaa gtataaaagg
     5341 aaaagatcat ttcctagtta aagtcaccgc cggtcgggaa tcacaccctg ccctgaagac
     5401 tcttttatca taacaaaaaa cgcttgcaag cgcaagcatt ttgttcattt tcatttttta
     5461 tttcaattta tccacttcat aggtatggac gagttcaaga cctgcaaagt tctgctggcg
     5521 aagagcctca tagacaatca tgcagacggt attagacaca ttgagactgc ggacatgttc
     5581 atcattcatg ggaatacgga gagccttctc aggatgttct cgcataaaat cttcaggtaa
     5641 gcccttgtct tcacgtccaa agagaaaata atggtcctcg tcagtcgata aatccacctc
     5701 agaatagatt ttttccgcga atttcgaaat cagatagagt tttcccttca tctgagatat
     5761 gaaatcttct aaactctcgt aaaaataaat ctctagctta tcccagtaat ccaatccagc
     5821 ccgcttcatc ttgcggtcat caataggaaa gcccataggc ttgatgatgt ggaggggaga
     5881 attggtcgca gcgcaagtac gcgcgatatt gcctgtattt tgtggaattt gaggttcaaa
     5941 taatacaatg tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa atagccacac
     6001 tgcccggagt caagctcagc aaacagcgtg gttaaggcat cgttaactta cctcacaaca
     6061 ggtttgaagt aaatcagcga aactactttc ttagtataac actttcagaa tcattgtcaa
     6121 tagaaatgac ttgatttttt caattttttc aagctatttc caagggttgt aaaatcgtcc
     6181 ctgattctgc aagataagta gtaaactaac tactaaaaac aaggttgcca agagcaaggt
     6241 aatatagtct ccttttttca aggcctgata actataccat gtgcgttttt tctctttccc
     6301 aaagcggcga agctccatgg cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat
     6361 caagggcgta ataatgagca gattgccttt gattcgttgc ataagagaag ctttcttgga
     6421 taattccatc ccacgcgcct cctgagacat cttgatagta aagaattctt cctgcaaatc
     6481 tggaatatag cgcaaggtca ggctgacaga ataagcaatc ttatagggca caccaatttg
     6541 atttaaactg gaagcaaact gactaggatg ggttgtcatc aaaaagataa tagccagagg
     6601 aatggtgcaa agatacttaa tggccaaatt tagcagataa aagagctcct ggctggttag
     6661 agtgtagaca ccgattccct gccaaatcac acttctctct ccataaagtc caaccccata
     6721 ctcgggagaa aagagataga ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac
     6781 ggctacaaag gaaacatctt taaagcgaat ttctgataaa tagaggagaa agactgaaaa
     6841 gatggcaatc agcaagagca ttctggtatc atagctaatc atggccgcca atgataccag
     6901 aatgaaaaag agaagtttcc cagctcctga caagcgatga atcacagtat ctctatgctg
     6961 gtaaccgatt aatttagctt gcatccctct ctcctttctt tgtaaaatgc cgttaaatcc
     7021 agtggatcca catctagttt cttagccaag ttaaagatgg aggtttcttt tagattggct
     7081 tttactaaca gctcaggatc gctcaacaga ctggctggaa cagtatcggc aatcaattct
     7141 ccatccacca tgacaaggac ccggtctgaa taatccagca tcaattgcat atcatgggta
     7201 atcatgacaa tggtatgccc tttttgatgt aactcttcga gaaattccat aatctcagta
     7261 tagttcttct gatcttgacc tgcagtcggt tcatctagga gaataatttc agctcctaag
     7321 accaaaattg aagcaatggt gacacgtttt ttctgaccaa atgacagggc agaaataggc
     7381 caattacgga attcataaag tccacagatt ttcaaggttt catatactct cgtttcaatt
     7441 tccttctcat ccacacctcg caaacggagc cctagagcca cctcatcaaa aatcatattg
     7501 gttgaaatca tttgattagg attttgtagc acatatccta ctcgttccgc ccgctctgca
     7561 acagaatcgc cttttatatc ctgtttttcc caaagatagc gtccttccgt ctgaataaag
     7621 ctacttatag ccttggctag agttgatttc cctgctccat tttttccgac aatagcaatc
     7681 ttttcaccct ttttaatatc taaatgtagg gattttaaaa tcggtctatc atcataagaa
     7741 aaagatactt cctctagtct aaagagtgac tgcaatgctg gggtttcttt tgccagttca
     7801 ttctgcaact gaacctgacc ttttgagata gacaagttat ccagattcgc taattgttct
     7861 tccttgacta agtccacacc taattgacgg agagtcgtta gataaagggg ttctcgaatt
     7921 ccattttgag tcaataaatc agtcgcaagc aactggtcag ggctcccatt aaaaaggata
     7981 cgaccatcgt ttatcaagac aatccgatcc acagggcgat gcagaacgtc ctccaaacgg
     8041 tgctcgataa taagagtcgt cgtcccctct tccttatgaa tctggtcaat caattcgata
     8101 atatcctgac ctgacttggg atctagattg gcgagtggct catcaaacaa gagaatcgga
     8161 ctttcatcaa tcaagacacc agccagactg actcgctgct tttgtccacc tgacaaatcc
     8221 tgaggacgct gatccagtaa aggaagaagg tccagctttt cagcccattt ataaacacga
     8281 cctttcatct catctagggc tgtcacatca ttttccagag caaacgccaa atcttctgcc
     8341 acagacaagc caataaactg cccatctgta tcctgcaaaa ctgtgctaac cagatgagac
     8401 ttatcataga tgctcatatc aaaggctgct tgacccttta tcaaaaattc tccatatgtc
     8461 tgacccttgt aaatattggg aataatccca ttcaaacact gacccaatgt agatttacct
     8521 gatccagatg gtccaacaat taagactttc tctcccttgt aaatggtcaa gtctatccct
     8581 tgcaaggtcg gttcttgttg tgtttcatac cggaaagaga aatccttcca ctcaattata
     8641 gcttctttca tcttactctc ttcattcgct tcttagactt ctattttatc ataaatcaag
     8701 cccttcttgc agtctcttct cttaaaatct tagcgccaaa aagattccta tcttagctta
     8761 cttgcctaac taatctataa acatcgaaaa agactagttg cccagccttc cccatcattt
     8821 tatactcttc gaaaatctct tcaaaccacg tcagtttcgc cttgccgtag gtatggttac
     8881 tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttc
     8941 tatccacaat ttcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt
     9001 tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttga gttcctgcat
     9061 aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa
     9121 caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg
     9181 gtgccaagac accccaaaca agggcatttg caagtagttg aatgagatta aaaataagaa
     9241 tatctttcca gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa agtcccacaa
     9301 ctaaaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa ccaacaagag
     9361 agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa
     9421 tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg
     9481 gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt
     9541 gtttaattgt aaatttgatt tccatactat tctcctattt tatccttcta ttttctttat
     9601 ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt
     9661 gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac
     9721 atctgcaaat gatttgccat agacaacctg attttgatag accagcatat cagcatgata
     9781 gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt
     9841 gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag
     9901 atgatcttct atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc
     9961 tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata
    10021 gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc
    10081 agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt
    10141 atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga
    10201 accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt
    10261 ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg
    10321 atgtattttt aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc
    10381 atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg
    10441 tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag
    10501 ggtatagttt taccttatag cgaattttca ctaaaagctc ttgttattag ctagtaggtg
    10561 cattttttct tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa
    10621 aattatcctt gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga
    10681 aaagcatgat gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg
    10741 ctttgatatc attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag
    10801 cgtccaagat tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc
    10861 agcagttcta gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga
    10921 atattctaac tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc
    10981 tgagctcttg gctgcccgcg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt
    11041 cgaacgcttt gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg
    11101 tctttgccaa atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc
    11161 gatagagagg gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga
    11221 tagaatcttt gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc
    11281 acgcgccgtg aagagccttc tcatcgttgg agcaggtaga attgcctatt atctacttgg
    11341 tattctcaaa gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag
    11401 cttctttagc gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga
    11461 tatcctgctg gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga
    11521 tgaggaaaat ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac
    11581 taaggtcaat cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat
    11641 cacacctaaa agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc
    11701 ccagtattca gaccttcaag ccatgcacca tctagccaat ggccaaatcg aaaccctgca
    11761 attccatatc aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa
    11821 aaaaggggtt cttattgcag ccattattcg aaagggcaag actattttcc caactgggga
    11881 ggatatgttg gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa
    11941 gatttatgac ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc
    12001 aaagttactt ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta
    12061 ccgtgaatcg agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt
    12121 aggcggttca gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt
    12181 cttgatcgtt gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt
    12241 ttctgggcaa atccccagcc ttattgatgc cttttttgaa attagttctg ggtttacaac
    12301 tactggagca agtattttga acgacgtttc ggttctcagc cgttccctcc tcttctggcg
    12361 aagttttacc cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga
    12421 caatgccaaa aatagccacc tagaggtgat gaaggctgag gttccaggtc ctgtttttgg
    12481 caaggttgta tccaaactaa aaaacactgc ccagattctc tatctccttt atctagctct
    12541 cttctccctc tttgtcatca tctattatct agctggtatg cctctctacg atagttttgt
    12601 cattgctatg gggacagcgg gaactggagg ctttaccgtc tataacgacg gaattgccca
    12661 ctatggcagc tcactgatta cctatctggt cagtatcgga gttctggttt ttggggtaaa
    12721 tttcaacctc tactactacc tcatgctccg tcgcatcaag gccttctttg gtgacgaaga
    12781 acttcgggct tacttggtca ttgtactggt ttctacaggc ttgattagcc tcaacaccct
    12841 ctacctctac ccagggtttt caaagagctt tgaaatgacc ttcttccagg tttccaacat
    12901 cattacaaca actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt
    12961 tatcctcctt ttcctcatgg caatcggtgg ctctgctgga tcaactgcag gtggactcaa
    13021 gattattcga ggcctcatcc tttcaaaaat tgccaaaaac caaattttgt caatcctatc
    13081 gccccaccgt gttttgaccc tccatgttaa taaaacggtg attgacaaag atacccagca
    13141 taagattctc aagtactttg tcatctatgc tatgattttg ctagcgctta tctttattgt
    13201 cagcctagat agcaatgatt ttctggtcgt gaccagcgct gtctttagct gtttcaataa
    13261 tatcgggcct attctaggaa ccacttctag tttctcaatc tttagtccca tctcaaaaat
    13321 tctcctatcc tttgcaatga ttgcagggcg cttggagatt tacccaatcc tccttctctt
    13381 tatgaagaga acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa
    13441 gagggattct tatattaaaa aagggggaac gtttttaaat ccaagcctat ctgtaagcaa
    13501 aatctttact caaataaaag atggaaaaac ttactacaca gcttggtaat gctccaatac
    13561 aaattgtaag gctttacttg gactctttgt taaatgaata tattccattc ctctggtagc
    13621 aactatctga atacctaatc tttctgcatc caccttcatc tctctataat aactccgaac
    13681 ttgtggagcc agaataatta aatcataaac acccataata tcataatgag ctccgtacgc
    13741 tcctgaattc gcaatcactc taacttctgc taattgagcg ccctcgttaa ttgcattggc
    13801 taattgcgca cttgttccag accctgcaca aagaaccagt actttcaact ccttactttt
    13861 tattacatct atctcaccag gcattatttc acttgtctcc tcctctaaaa taatatcatt
    13921 tgagcttaca acatcttctt tcatcagtaa ctgtctatca tacgctctac agaatggcaa
    13981 ataaatcaat atgtcgacaa ctaacatcaa agataaaaat acaaaggaga tagattgaaa
    14041 attcgttcca attaacaatc ccaagggacc aggaaaggcc caaggtaact ggatataaaa
    14101 tccattcatt ccaaagaaat caataaagac ctttcctaga aatacattta ctggtggagt
    14161 catcaaaaaa gggacaaaaa gatagggatt caaaataaca ggcataccaa atagaagagg
    14221 ttcatttacc gcaaataaaa caggagtaat tgtagcttta cctaccgatt ttaattgttt
    14281 agaccgcata aagaaaatca aaataaatgg tacaacaaag gtagcccccg ttcctccaat
    14341 agcagcgata taattcccga aattaggtgt taaagaatga taagggaact gcccatttgc
    14401 aagaagttga gcattctctt ccatattgct cagttgtaaa gctgtaacag caggtaagac
    14461 aatagatggt ccatgaattc caacaaacca aagcaaagca cacataaacc aaatcaacat
    14521 catagcagga tatgattcta cccccttaaa aataggagtc aatagttgtt gaaatacttg
    14581 ggcaaaagga acatctaaac taaaccgaga tacaatatct aacaaaccac taataagtaa
    14641 aacaaaagaa aaaggaaaaa tatctctaaa agcttgtgat atagccccag gaacttcctt
    14701 aggtaaatga atagtaatgt ctcgcttaat acagaatcgg taaacattta cagtaataaa
    14761 ggcactgaca aacgaagtta ccaatccttg agatcctaaa tagtttgtcg acaaacttgt
    14821 agatcccgtc ttctcatcaa ctacaagcgt tacagttaat accagataac tacatatggc
    14881 tgccaacatt acagaaatat catttattac ctttccagga ggcatttttc tattaacatt
    14941 tccagccaat gactttgcaa ctgtccctga aacaataaca cccaaaactc ccatggttaa
    15001 catatacact tttctcatcc agacaatagc tggactcggt aactctactc cgaaattttt
    15061 aggaatcata ataataatca tcaaaaagct tgaaaacata attaaaggca tagtagcaat
    15121 gaatcca
//
LOCUS       DAVHIU010000041        14842 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVHIU010000041 DAVHIU010000000
VERSION     DAVHIU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.043"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(41..376)
                     /locus_tag="VVN06_001081"
     CDS             complement(41..376)
                     /locus_tag="VVN06_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562642.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(467..1777)
                     /gene="der"
                     /locus_tag="VVN06_001082"
     CDS             complement(467..1777)
                     /gene="der"
                     /locus_tag="VVN06_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5562643.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(1791..2504)
                     /locus_tag="VVN06_001083"
     CDS             complement(1791..2504)
                     /locus_tag="VVN06_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU5562644.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(2501..3397)
                     /gene="dnaI"
                     /locus_tag="VVN06_001084"
     CDS             complement(2501..3397)
                     /gene="dnaI"
                     /locus_tag="VVN06_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU5562645.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3398..4567)
                     /locus_tag="VVN06_001085"
     CDS             complement(3398..4567)
                     /locus_tag="VVN06_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU5562646.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSPKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(4568..5041)
                     /gene="nrdR"
                     /locus_tag="VVN06_001086"
     CDS             complement(4568..5041)
                     /gene="nrdR"
                     /locus_tag="VVN06_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU5562647.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5181..5546
                     /locus_tag="VVN06_001087"
     CDS             5181..5546
                     /locus_tag="VVN06_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5562648.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            5551..6246
                     /locus_tag="VVN06_001088"
     CDS             5551..6246
                     /locus_tag="VVN06_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562649.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6263..7030
                     /locus_tag="VVN06_001089"
     CDS             6263..7030
                     /locus_tag="VVN06_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562650.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7383..8582)
                     /locus_tag="VVN06_001090"
     CDS             complement(7383..8582)
                     /locus_tag="VVN06_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562651.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(8579..9472)
                     /locus_tag="VVN06_001091"
     CDS             complement(8579..9472)
                     /locus_tag="VVN06_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562652.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            9589..9669
                     /locus_tag="VVN06_001092"
                     /pseudo
     CDS             9589..9669
                     /locus_tag="VVN06_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10154..10373
                     /locus_tag="VVN06_001093"
                     /pseudo
     CDS             10154..10373
                     /locus_tag="VVN06_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            10788..11405
                     /locus_tag="VVN06_001094"
     CDS             10788..11405
                     /locus_tag="VVN06_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU5562653.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQVGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(11725..12612)
                     /locus_tag="VVN06_001095"
     CDS             complement(11725..12612)
                     /locus_tag="VVN06_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5562654.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(12761..14644)
                     /locus_tag="VVN06_001096"
     CDS             complement(12761..14644)
                     /locus_tag="VVN06_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU5562655.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVVHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         4321 a         3286 c         2777 g         4458 t
ORIGIN      
        1 gtaccataag ccacctccta caaatagaaa ccgatataaa tcaatgcctt ccacccttag
       61 acttccctag ttcctgtctc aagcgaaaca tttctttgaa acaggaataa gttaaccaat
      121 tcataccaat agctagcaga ataaaaagaa accaaatgcc ccataacttg atatctgtca
      181 catttctcaa gacggtattg aaaaacagaa ctgaaacaac tgtccaagca aggctaaaaa
      241 gagaatagaa ggggatgtaa aaccagtaaa aataataaaa aattggaaaa aacttactat
      301 ttctgttggc cttttcaatc cagttatcaa aataaaagta cggtgctaaa agtaagaatt
      361 taaacaaatg ttccatcacc gacatcaccc tccttctttt gatagcgttt tctattattt
      421 tattatatca aaaaaatccg gaactgtcat tccagattct acttttttat ttgcgttttc
      481 ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc cttgcggatt tgattttcca
      541 agaaacgcag gtaagaaaag tgcatgagtt cttcttcatt gacaaagatg acaaaggttg
      601 gtggtttggt tgccacttgg gtcgcataga aaatcttgag acgttttcct ttgtctgtcg
      661 gtgttgggtt gatggcaatg gcatccatga tgacatcgtt caagacagct gatggaatac
      721 gtgtattttg actttcgctg atttgcttaa tcatctcagg aagtttgtgg agacgttgct
      781 tggttaaagc tgatacaaag ataatcggtg cgtaaggcag gtattggaac tgctcacgga
      841 tatcttcttc ccagtttttc atagtgtggt tatctttttc aagcgtatcc cacttgttga
      901 ccacgataat catcccttta ccagcttcat gggcaaatcc tgcgatacgc ttgtcgtact
      961 cacgaatgcc ttcttccgca ttgatgacca tcaagaccac atctgaacgg tcaatagcac
     1021 gcatggcacg cataacagag tatttctcag tattttcata aaccttacca gacttacgca
     1081 taccagccgt atcaatcatg gtaaactctt gaccatctgt atctgtaaag tgggtatcaa
     1141 tggcatcacg agttgttcca gcaacaggac tagcaataac acggtcttcg cccaagatag
     1201 cattgatcaa gcttgatttt ccaacgtttg gacgaccaat caggctaaac ttaatgacat
     1261 ctggattttc ttcttcatat tcatttggaa ggttttccac aatcgcatct agtacatccc
     1321 ctgtaccgat accatggaca gatgagatag gcagtggttc acccaaaccg agggcataga
     1381 aatcatagat atcatttcgc atctcagggt tgtccacctt gttaactgcg aggataactg
     1441 gtttgtgggt cttataaagc ttacgggcta cgtattcgtc tgcatcggta attccttcct
     1501 taccggacac gacaaagacg ataacatctg cttcttccat ggcaatttct gcctggtgct
     1561 tgatttgttc catgaaagga gcatcgacat catcaattcc tcctgtatca atcatgctaa
     1621 aagaacgatt gagccactca cccgttgcat aaatacggtc acgtgtcact ccttcgacat
     1681 cttctacaat ggagattcgc tcaccagcga tccgattaaa tagggttgat ttcccaacat
     1741 tgggacgtcc tacaatggca atagttggta gggccataat ttctcacttt ctacaataat
     1801 ttcttctgtt caagattttt tctagttgag cttggttcag cttgaccaaa ctgttctgct
     1861 aggcgctgac tccagcttgt ggtcgcacgc gccccagcat agtcagcctg aacacggtca
     1921 taagcttgga ttgcctcagt tgactgttct tggtattctt cctcaaagac aacattctct
     1981 agtggcagtc tcggtttcat atcatgatgt tgatttggca cacccagtgc catcccaaag
     2041 acagaatagg tgtagtcagg caggttaaag agctctgcca cttcttcaga cttgtatcga
     2101 accaaaccga taatcacacc accatagccc aagctttcag ctgccaacaa ggcgttttgt
     2161 ccagcaagag ctgcatcgac cgaactaatc aagagacctt ccacaccttg gggttggaag
     2221 gtgtcggtat gaagtcgggc tcccttttct gctcggttca aatctccgac aaagagaagg
     2281 aaaacagcag actggcgaat ggcttcttga ggtaccaatt catacaaggc atctttcttc
     2341 tcttgacttc gtaccacaat cacagagtag gattggaaat tcttccaaga tgatgccatc
     2401 tgggctgctg tcaaaatctc atttaagtct acttggggaa tttcttgctc tttaaacctg
     2461 cgcactgaag tatgagcctt catcaattta atggtttctg tcatcgacgg tttactcctt
     2521 ctaaacgagt ctcctcagcc aaataacgga tgcgttccat gacccgtctg gcttcccagg
     2581 tttcgtcatt tccatgtttc actttcgcaa aatgcttctc caaatcttca aagttgaagt
     2641 tggatgtgaa aaaggtcggt aaattttcct gcatccgata ttggagaatg acctgcagga
     2701 tttcgtcacg cacccaaacg gttgattgct cggcgccaat atcatctaaa atcaggacct
     2761 cagacagctt aatctcatcc accaaggtct taacattgcc atcactgata gcatttttga
     2821 catcaatgac aaagctagga tagtggagga gagttgatga aacaccacgt ttttctgata
     2881 aatcatgagc taaggccgcc accatgaaac ttttacccac accaaagtct ccatataagt
     2941 aaagaccttt tcgaatagct ggatattgct ccacgaaggc tagtagcttt tcaaaaactg
     3001 gtaagcgccc caaatcatcc aagtcaactt gagccaaact agctttcttg agactggctg
     3061 gtagattgat taacttgaga cggttcttaa tagccgcttc tttttcagcc gcgattagct
     3121 caggagtttc ttcatatgaa acatctgcat agccatgatt cttaaccaaa atcggcttgt
     3181 agcctttggc aatataatcc gtatccccac ggagaaactt gtcacgctcg gtgatgtact
     3241 gattaaactt ggagatactg cgatttaatt cctctggagt caaggattct tgctggataa
     3301 aagccgcaac atcagggtcc ttcatgattt tctggaccaa atcttgataa taaaaacggc
     3361 tgggttgacg tttgagtacg tctccgacac tttccatcta atctcctcct ttttctaatc
     3421 gagctaatag ttcttgcttc ttacgttcta gttccagacg agtttcctcg ctggtttcat
     3481 tcttatattc aggattactc catttaggaa cattggtttt tactggggca gtctgattct
     3541 gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg caagactgcc ttttctgccg
     3601 aatgaatctt ttgataggca tagtcattgg ctaccttcat ggcatatttc tcattgatat
     3661 ttgccgaatc gaccttatta aaggtcaata aaagaatgat attgatgact tcgtccagca
     3721 agcccaagcc agccatctgt tgcaagagtt ctctttctgt ttgggtaatg gttcccttgc
     3781 gtgtttgctt gatttctgcc aagaactgca gggcagtttt acttttagct tccttgataa
     3841 tggttgtttc cttaggacta aagtccgagg aaactggttt ttgagcaatt ttttcccgca
     3901 tgcgtttggt tgaaataacc tgggaaacag ctgttgactt ggccaattga taggtctcaa
     3961 accaagtcca cttcttctcc tcagcaatag caaacaagtt caagacatca gactgctcat
     4021 ccgcaaaacg aagtccatct cgagccatca gctgacgaaa atgatccaag tcaaaatcat
     4081 tggccacttt cttcttgaga ccaaggtctt cttgactgcc tagttctgcc aattctggaa
     4141 agacttgatt gagtgagaca ggtatttctt caccatcagc actttcaact ttcaaatcct
     4201 ccacagcaga gtcgccaatc tttttctcta agagtctgcg ataaacagga tgccccaaga
     4261 agtcttgact agatagagga gcatggaggg ctaactgata aacatccccc ttttgataga
     4321 gggtcaagag attaaaagca gataagattt tcaatgattt tatcagtcta tccatcccaa
     4381 agttgagatg attgaggata cttgaaaaaa gatattcctt tctaccatta tcccaaaaac
     4441 tgattgtata aagataaagg ctcagtgcct cctgaccgat aatcgggagg tagcactgta
     4501 ccagagatga ggtatcttgc gacacccgat tattctttag ataagaaaaa cggtcaattg
     4561 gcttcattta tctttccttt ttctttttag aggactgggt gatttgttgg agcaagctct
     4621 ctaactcact gacatcctta aaactacgat agacactagc aaaacgtaca taggtaatct
     4681 cgtccaattc agccaactcc tccatgacga gtgaaccaat gtcctcactt tgaatttcat
     4741 tttcatttcg accacggagt ttctgttcga tacgattgac taccatgttg atttcatcac
     4801 ttgacacagg acgtttctgg gctgagcgga taatcccatt aaagatttta tctctggaga
     4861 attgttcccg tgtgccatct tttttaacaa ccactaaggt tctttcttct actcgttcgt
     4921 aggttgtaaa acggtgttgg cactcgtcgc actctcgtct tctacgaatg gtgttccctt
     4981 cttctgcttg gcgactatcg ataacacttg acttggtagc cccacatttt ggacaacgca
     5041 tcctttccct ccttatcgtt ttcttttcat tataccattt tttaagcgat tcccaaaaca
     5101 attcttcttt ttgcttgaca agttttttgt tttgttgtat tatttaatta agacaaaaga
     5161 ataagagaaa ggagactaag atgtcctgga catttgacaa caaaaaaccc atctatttac
     5221 agattatgga gaaaatcaag cttcagattg tttcccatac actggaaccc aatcaacaac
     5281 ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa tcccaatacc atccaaagag
     5341 ccttatcaga ccttgaacga gaaggatttg tctacagcaa gcgaacaact ggacgatttg
     5401 tgactaagga taaggagcta atcgctcagt cacgcaaaca attatcagaa gaagaattgg
     5461 aacacttcgt ttcctccatg acccattttg gctatgaaaa agaagaacta ccaggcgtag
     5521 tcagtgatta tattaaagga gtttaagcct atgtcattac tagcatttga aaatgtatcc
     5581 aaatcatatg gagcaacacc agcccttgaa aatgtttctc ttgacattcc agctggaaaa
     5641 attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa ccctgattaa actaattaat
     5701 ggcctcttac aaccagatca aggacgtgtc ctcatcaacg acatggaccc aagcccagca
     5761 accaaggccg ttgtagctta tttgcctgat acgacctatc tcaatgagca aatgaaggtc
     5821 aaagaagccc taacctactt caagaccttc tataaagatt tcaatcttga acgcgctcat
     5881 catctacttg cagacctggg cattgatgaa aatagtcgtc tcaagaaact atcaaaagga
     5941 aacaaagaaa aggttcaact gattttggtt atgagccgtg atgctcgtct ctatgttttg
     6001 gatgaaccca ttggtggggt ggatccagca gcccgtgctt atatcctcaa taccattatc
     6061 aacaactact caccaacttc taccgttttg atttctaccc acttgatttc tgatatcgag
     6121 ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag tcgtccgtca aggaaatgta
     6181 gatgatattc gctacgagtc aggtgaatcc attgaccaac tcttccgtca ggaatttaag
     6241 gcctaagcaa aggagattat ttatgttttg gaatttagtt cactacgaat ttaaaaatgt
     6301 taacaagtgg tatttagccc tctacgcagc cgtgctagtc ctttctgccc tcatcggaat
     6361 acagacacaa ggctttaaaa atctacctta ccaagaaagt caggctacta tgctactttt
     6421 tctagctaca gtctttggtg gcttgatgct tacacttgcg atttcaacca ttttcttgat
     6481 tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc tatctgactt tgaccttgcc
     6541 agtttctgaa caccatatca tcacagccaa actaatcggt gcctttatct ggtcattgat
     6601 tagcaccgct gtattggctc taagtgctgt tattattctg gctttaacag ctccagaatg
     6661 gattcctctt tcttatgtga ttacatttgt agaaacacat ctccctcaga tctttcttac
     6721 aggtatatcc ttcctactaa ataccatttc aggaatcctc tgcatctacc tggctatttc
     6781 cattggacag cttttcaatg aataccgtac agcactcgct gttgcagcct acattggtat
     6841 ccaaatcgtc attggattta ttgaactttt cttcaatctt agttctaatt tctatgtcaa
     6901 ttcactggta ggactcaatg accatttcta tatgggagca ggtatagcca ttgttaaaga
     6961 actcatattc atagctatct tttatctcgg aacctactac atcttgagaa ataaggttaa
     7021 tttgctttaa ataattttta cctagatatg taacatactc atagaacaaa agagaccagg
     7081 caaaaagtct ttaaaattag aaaacgcata gtatcaagtg ttaaaaatcc ttgatattat
     7141 gcgttttttg tgagttgact tatttccttt cactatatcg caaaatgaaa taagaacgga
     7201 acgatgggat tttggaattc aaatcaattt gtaagaatgt tttagaagta atattatcct
     7261 attccagatt cagttcacta tacaattgag ttttcaagca acttgtttac ataatgtgta
     7321 cataattagg ttcgtgattc cacccttttc acctttaaaa acctcgcttt cgcaaggctc
     7381 ttctatttat aagataaggc acgtttaaag gttttccaaa tccctaaatc atccgtttga
     7441 agaacgagac tagcatacat gcgtccgata aatcctgttg ctaccaccgc aaaaatcact
     7501 gtaatagcaa gtgaaatcca tgcttccgct ccccccgcat agccattaat cgttcgaaac
     7561 ggcataaaga aggtcgaaat aaagggaata taagaaccaa tcttcaagag gagattgtca
     7621 ccagctgcac ctagagctgt cactccaaaa aaaccaccca taatcaaaat catcaaaggc
     7681 gacaaggctt tccctgagtc ctcaggacga gaaaccatag atcctaggaa ggctgccaag
     7741 actacataca tgaaaagact gatcaaaata aagagcaggg tattcagtga gatagcatct
     7801 cccaagtgat ccaaaatacc agactgagcc aagaatggca aatctttaaa gagcaaaacg
     7861 gcagccagac cacctacaac atagatccca atatgcgtta aaatcactag aaacagagcc
     7921 atcatccgcg catagaaata gtgacttgcc cttatgctag aaaaaacgac ttccataatt
     7981 ttggtgcctt tttcactggc aacttcctga gctgttacac ccgcataggt aatcagaatc
     8041 atataaagaa agaatcctaa ggcacctgct gcaattgttt gaataaactt tttattttcc
     8101 ttggcttcat caatcttttc tgtgaattga attgtctgcg ctaagcgttt ttcctgctct
     8161 tgagacaagg aagcagttga acgattaagc tgattttgca gttcattgag tgtacctgta
     8221 acctcaaatt taattccatt ttcaagcgat gtttcgccat gataaactgc ctttagaaca
     8281 ctatcttctt gatcaatggt caaataacct tttaattttt cttctttaat tgcttctttg
     8341 gcacttgctt cgtctttata gtcgaagtta acaccattta cattcttcag tccttctgct
     8401 acagatggca ctgttgtcac tactgccact ttattatttt tagccataga agaaccttgg
     8461 agatgcccaa ttcctacaga gattcctaaa aagaggaacg gcgaaatcac cataaagaag
     8521 aaactccatg actcgacatg tcgaagatag gtttccttga ttacaaccca catatttctc
     8581 atacttccac tcctgattct agtttaaaga tttcatcgat agttggcgct tgttggtcaa
     8641 atgttgcgat atattgacct tgagtcaaga ttgggaagag ttcccttcca gcgctctcat
     8701 cctccaaaat caatttccaa ctgccttgtt tggtcaagct cacctgtttg acatgaggaa
     8761 gattttccaa ttcttccttg cttcgttcac ttgaaacaaa gagacgcgtt ttcccgtatt
     8821 gattgcggac atcctgaact ggtccgtgca agaccacacg gccatctcgg atcatcagaa
     8881 tatcgtcaca aagttcctca acattggtca tgacatggtc agaaaagata atggttgctc
     8941 cgcgctcttt ttcctgaaaa atgacttgtt tgagcaattc tgtattaact gggtccaatc
     9001 cactaaaagg ctcatccaag ataatcaggt ctggttcatg aatcagagta ataatgagct
     9061 gaatcttctg ctgatttcct tttgacagac tcttgatttt atctgtcagc tttcctttca
     9121 cttccaacct cttcatccat tgagggagtt tttctttgac ttctttggca tccatgcctt
     9181 ttagagtcgc caagtagcga acttgttcaa gaactgtcaa tttaggcatg agactgcgtt
     9241 cttcaggcag ataaccaatc cgagcatagg tctcctgacg aatatcctga ccatccagac
     9301 cgatttctcc ctgatattct aggaatttca aaatactatg gaaaatcgtt gtttttccag
     9361 caccattttt tccgactagt cccaaaatac gacctggtcg cgcttgaaag tcaataccaa
     9421 acaaaacttg cttggatcca aaacttttct ctagacttct tacttctagc atctttcacc
     9481 tccgaaattt ctttcactca ttatactcct ttttgatagc ctttacaatg atttctgtcc
     9541 atttttagaa gactattgct gtgtaaaata tagcctggag cacttttata ctcaatgaaa
     9601 agcaaagagc aaactaggaa gctagtcgta ggctgctcaa agtacagctt tgaggttgca
     9661 gataaaactg acgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgaa
     9721 gtcagtaact atatctacag caaggcgaag ctgacgtggt ttgaagagat tttcgaagag
     9781 tattagtgat aaatccatta tacagtagca aactcaattt ataccttccg ctcctcaact
     9841 gtctattttt aatcctgaat tgttatttga gtaactcctt tttcctcata aagttttctt
     9901 cctctaaaac ttctggaaaa aggcgaatag tttcagacaa catttttata agaaacaagt
     9961 tcatctgtca tttcaagaag gagtaatcct ttatttacta atggacggaa cggaattcaa
    10021 ccgcttgtcc gatatgtttt ctaaggatta tatagtaaaa tgaaataaaa acaggacaaa
    10081 tagatcggga cagtcaaatc gatttctaac aatgttttag aagtagatgt atactattct
    10141 agtttcaatc tgctatatct attatgcaca cccctatagg atctaatgaa aatcacaaca
    10201 ggctcattca tagatggtta cctaagccta agggaactaa gaaaacgact acaaaggaag
    10261 tcgcattcat cgaaaagtgg attaacaact atcctaaaaa atgcttgaac tacaagtccc
    10321 ccagagaaga cttctggatg actaacttga acttgaaatt tagcaataat taattcacca
    10381 tctaactata tttagtaatt atttcagaac tgattaatat taaaattaac taacgattca
    10441 aaggattcat actagccata aattacgtcc atcagagaga gactcttact acttttagat
    10501 tttagtcttt ctagcttcag aatacatcta aactttaggg aaaatgacta ttcgaaagcg
    10561 cgaatgcctc aaaattatct cagataagct attcgaaact tagaatgctt ttaaatttat
    10621 ggaattgcga ttattcgaaa cctagaatgc atataacctt tagttgacag acctattcta
    10681 agtctcgaag ggctatttac tttctattcc ttatcaaaaa agactcattc cccctttctc
    10741 ctccaaaata tggtatagta gaaatatact atctatgagg agtttacatg tcacaggata
    10801 aacaaatgaa agctgtttct ccccttctgc agcgagttat caatatctca tcgattgtcg
    10861 gtggggttgg gagtttgatt ttctgtattt gggcttatca ggctgggatt ttacaatcca
    10921 aggaaaccct ctctgccttt atccagcagg taggcatctg gggtccacct ctctttatct
    10981 ttttacagat tttacagact gtcgtcccta tcattccagg ggccttgacc tcggtggctg
    11041 gggtctttat ctacgggcac atcatcggga ctatctacaa ctatatcggc atcgtgattg
    11101 gctgtgccat tatcttttat ctggtgcgcc tatacggagc tgcctttgtc caatctgtcg
    11161 tcagcaagcg cacctacgac aagtacatcg gctggctaga taagggcaat cgttttgacc
    11221 gcttctttat ttttatgatg atttggccca ttagcccagc tgactttctc tgtatgctgg
    11281 ctgccctgac caagatgagt ttcaagcgct acatgaccat catcattctg accaaaccct
    11341 ttaccctcgt ggtttatacc tacggtctga cctatattat tgactttttc tggcaaatgc
    11401 tttgacacgt aaaaaatccg tttggtttcc caagcggatt tttaaagcgt agattaacta
    11461 tagcttgata ctaaatatac tttggtatgg aaattatgca tatttttcga tagtgaggcg
    11521 aggacttacc tagcctttcc gccgtgatag aaacacctga aatctaatgg tttcaggtgt
    11581 tcggaaactt tgagacatta ggatcaaagt ttaggtatgg aatttcgaag aaagtcgcta
    11641 ccgtccgtaa tcacttaagg aaaggctcaa aaatattgtt ttcaaccaca aaatccgttt
    11701 ggtttcccaa gcggattttg tgctttattt tgaaacttct tttgcaagaa caaagttccc
    11761 aagtgtggca gaaccatttc ctgcgactgc tggcgtcacg atatagtcac gcacatctgg
    11821 tactggtagg taaccattaa gaagagatgt aaatttctca cggacacggt ccagcatatg
    11881 ttgttgagcc atgacccctc caccaaagac aatcacgtct gggcggaaag tcactgtcgc
    11941 attaaccgca gcttgagcga tatagtaggc ttgaacatcc caaacagggt tgttgagttc
    12001 aatattttcc ccacgtacac ctgtacgagc ttccaaactt ggaccagctg cataaccttc
    12061 tagacatccc ttatggaaag gacaaacacc cttaaactct ttttcaatat ccattgggtg
    12121 tctagcaaca taataatgac ccatttcagg gtgacccaca ccaccgataa actcaccacg
    12181 ttggatgaca cctgcaccga tacctgtacc gattgtgtag taaaccaagt tttcgatacg
    12241 accaccagca ttgttacggg caaccacttc accgtaagca gagctgttta cgtctgttgt
    12301 gaagtacatt ggcacgttta gggcgcgacg aagggcacca agcaagtcca catttgccca
    12361 gtttggtttt ggagtcgtcg tgataaagcc ataagttttt gagtttttgt caatatcaat
    12421 cggcccaaat gaaccaactg caagaccagc aaggttatcg aattttgaga agaactcaat
    12481 ggttttatcg attgtttcga ttggagttgt tgttggaaat tgtgtttttt ctacgacgtt
    12541 aaagttttca tcaccgacag cacagacaaa ctttgtaccg cccgcttcca agcttccata
    12601 taattttgtc atgataaacc tcttgttttt attttcttta ttatagcata cttcgaaagt
    12661 ctaaatgtct ctatttttta gattttcctc tgtaaatctt actatctaat aaaaacgaac
    12721 aaacatgtca tttgttcgtt ttcacattag agaggattga ttagattttc acttcgatca
    12781 cagcatcccc cttcgcaact gaacctgttg cgactggagc tactgaagcg tagtcagctg
    12841 tatttgtaac gataaccatt gttgtatcat caagtccagc tgcagcgatt ttgtttgagt
    12901 caaatgttcc aagaacatca ccagctttca ccttattacc ttgagcaact tttgcttcaa
    12961 aaccgtcacc gttcatagat acagtgtcaa taccaacgtg aatcaaaact tcagcaccat
    13021 ttcttgtttt caaaccaaaa gcgtgccctg ttggaaaggc aattgaaact tcagcatcag
    13081 ctggtgcata gaccacgcct tggcttggtt tcacagcgat accttgtccc atagctccac
    13141 ttgagaagac tgggtcattg acatcagcaa gagcgacaac atcaccgacg ataggagtta
    13201 caagtgtttc attttgaaga gctgctggcg caacttcttc tttttcttca gccacttcag
    13261 cttgttttgc agctgcagtt gcgtctactt catcttcgta accaaacatg taagtaagag
    13321 caaaaccaag ggcaaatgat acagctacca taagaaggta ttgtggaagt tgtccgttac
    13381 caacataaag cattgtacca gggatgatgg tgataccatt accagtacca gcaagtccaa
    13441 ggatagaagc caatccacca ccgattgcac cagcaatcaa tgaaaggaag aatggtttac
    13501 ggaagcgcaa gttcaccccg aagatagcag gctctgtaat acctaggaag gcagaaagag
    13561 cagccgggaa agcaagtgtt ttcagttttg gattttttgt tttaacacca accgcaacag
    13621 tagcagcacc ttgagctgtc atagcagctg tgatgatagc gttgaatggg ttagcatggt
    13681 cagcagcaag taattgcact tcaagcaagt tgaagatgtg gtgcacacct gacacgacga
    13741 tcaattggtg aaccccacca atcaagaaac caccaagacc aaatggcatg ctaagaatcg
    13801 cttttgtagc aataaggatg tagttttcaa caacgtggaa aactggtcca atgacaaaga
    13861 gtccaaggat agacatgacc aaaagtgtca cgaatggtgt taccaatagg tcaatgacat
    13921 ctggaacaac cttgcggaca gctttttcaa atttagctcc gacaaccccg atgatgaagg
    13981 ctggaagaac ggaaccttgc aaaccaacaa cagggatgaa accaaagaag ttcatcgctg
    14041 ttacttcacc accttgagca actgcccaag cgtttggaag tgagccagag acaagcatca
    14101 taccaagaac gataccaacg gcaggatttc caccaaatac acggaaggtt gaccacacaa
    14161 ccaaacctgg caagatgatg aaggctgtat ctgtcaagat ttgtgtgtaa gttgcaaagt
    14221 cacctggaag tggcatttca agagcgttga aaagaccacg cacacccatg aagagacctg
    14281 tcgctacgat aactgggatg attggaacga aaacatcacc gaaagtacgg atagcacgtt
    14341 ggaaccagtt cccttgttta gcaacttctg ctttcatgtc atccttagat gatgttggta
    14401 agccaagtac aacaacttca tcgtacattt tgttaactgt acctgtacca aagataattt
    14461 ggtattgccc tgagttaaag aaagcacctt gaactttttc caagttctca atcacttctt
    14521 tattgatttt ctcttcatct ttgaccatga cacgtagacg agtcgcacag tggacaacac
    14581 tattgacatt ttcacgtccg cccaaggcat cgatgacttt ttttgcaatt tcctgattgt
    14641 tcatttgcaa aaatctcctt atataacatt ttgttcttgt ttgaaagcga ttttattcgc
    14701 ccttacgact attattttat catgttttaa aaatatgtca agcgttttgc agaaaaaata
    14761 ttctatccac ttagtgggca agctttagat tgattgtttt tgactgtttc atcaggaatt
    14821 ccttgaaaaa taagtttgaa ac
//
LOCUS       DAVHIU010000042        14707 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVHIU010000042 DAVHIU010000000
VERSION     DAVHIU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.044"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..337)
                     /locus_tag="VVN06_001097"
     CDS             complement(<1..337)
                     /locus_tag="VVN06_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5562656.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTEN"
     regulatory      366..462
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            460..969
                     /locus_tag="VVN06_001098"
     CDS             460..969
                     /locus_tag="VVN06_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU5562657.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     gene            1069..1141
                     /locus_tag="VVN06_001099"
     tRNA            1069..1141
                     /locus_tag="VVN06_001099"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1102..1104,aa:Thr,seq:ggt)
     gene            1484..2560
                     /locus_tag="VVN06_001100"
     CDS             1484..2560
                     /locus_tag="VVN06_001100"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5562658.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            2616..3551
                     /gene="dapA"
                     /locus_tag="VVN06_001101"
     CDS             2616..3551
                     /gene="dapA"
                     /locus_tag="VVN06_001101"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5562659.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     IPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(3598..4971)
                     /gene="mnmE"
                     /locus_tag="VVN06_001102"
     CDS             complement(3598..4971)
                     /gene="mnmE"
                     /locus_tag="VVN06_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU5562660.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5107..5289)
                     /locus_tag="VVN06_001103"
     CDS             complement(5107..5289)
                     /locus_tag="VVN06_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU5562661.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            5413..6003
                     /locus_tag="VVN06_001104"
     CDS             5413..6003
                     /locus_tag="VVN06_001104"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5562662.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            6000..7079
                     /gene="prfA"
                     /locus_tag="VVN06_001105"
     CDS             6000..7079
                     /gene="prfA"
                     /locus_tag="VVN06_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5562663.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMLEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            7079..7918
                     /gene="prmC"
                     /locus_tag="VVN06_001106"
     CDS             7079..7918
                     /gene="prmC"
                     /locus_tag="VVN06_001106"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5562664.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            7902..8504
                     /locus_tag="VVN06_001107"
     CDS             7902..8504
                     /locus_tag="VVN06_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU5562665.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            8506..8937
                     /locus_tag="VVN06_001108"
     CDS             8506..8937
                     /locus_tag="VVN06_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562666.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            8999..10255
                     /locus_tag="VVN06_001109"
     CDS             8999..10255
                     /locus_tag="VVN06_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5562667.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            10263..11237
                     /locus_tag="VVN06_001110"
     CDS             10263..11237
                     /locus_tag="VVN06_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU5562668.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            11237..11851
                     /gene="pmp23"
                     /locus_tag="VVN06_001111"
     CDS             11237..11851
                     /gene="pmp23"
                     /locus_tag="VVN06_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU5562669.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            11925..12899
                     /locus_tag="VVN06_001112"
     CDS             11925..12899
                     /locus_tag="VVN06_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU5562670.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            12973..14604
                     /gene="rlmD"
                     /locus_tag="VVN06_001113"
     CDS             12973..14604
                     /gene="rlmD"
                     /locus_tag="VVN06_001113"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5562671.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
BASE COUNT         4477 a         2848 c         3163 g         4219 t
ORIGIN      
        1 tgttttcagt tacgaattgc cctataatgt gaggaaattg ggttacaaag agaaaagtta
       61 aaaacaagtc taacagaaaa gtagaaaggc gagcaagaga aaatttcact aaacggataa
      121 gtctattttt ctttttttgc ttaaatacaa tcgtatcatt tgtgataaag gcaaataaga
      181 taccgataat atttgccaag gccgtagcaa ggagttcctg atgacttagt tgataaatga
      241 ctagtcgtga gagaatcgaa actagagttg tcgcaaggcc aaaaaagaga taggctaaga
      301 tttcattgtt aaaaaatttt tgaataggtt ttttcatgct ttaagtatag cataaagcag
      361 gctattgtgc tatactagta aggttgaatg aatcaaccct tggtgcttag cttctttcac
      421 caagcatatt acacgcggat aaccgccaaa ggagaaaaga tgaaaaaatt aactattcgt
      481 gatgttgcag atattgcaat cgtcgctgct atctacgtcg ttttgactgt cacaccgccc
      541 ctaaatgcca tcagctatgg tgcttatcag tttcgtattt cagaaatgat gaactttatg
      601 gctttttaca atcctaagta catcatcgga gttaccatcg gttgtatgat tgctaatttc
      661 tttagcttcg gactgctaga tgtctttgtt ggtggtggtt caaccttagt attcctcagt
      721 ttaggtgttt ggctttttgc aaaatatagc aaggattacc tctttaacgg attgattcga
      781 aaagatcatt tcttcttttc aatcctcttt tcaatttcca tgtttactat cgctgcagag
      841 ttaaatattg tagcagaatt accattcttc cttacttggt ttacaacagg aatcggtgaa
      901 tttgcttcat tgattattgg agcgattatt attggtaaaa ttggtcgtcg aattgattta
      961 agtaaataga agaagataag ctaggtagtt cttacctggc tttttcctat ggaaaatttc
     1021 taaggaaact tgacaagttt ttctgaatat agtatacttc ttaagtaagc tgatttagct
     1081 cagttggcag agcgcatcca tggtaaggat gaggtcgccg gttcaatccc ggcaattagc
     1141 atgatatgta tagggaaact cttgaatacg agagtttttt gttatactcg atcgaaaaaa
     1201 tttcttattg acaaaatcaa gtatctctag taatatagaa tatgcgatga gtcgataggt
     1261 agtcttcgga ctactattga gcataaggag gtcataacgc aggagcggac cttgatgagt
     1321 tgtgtgaacc tgctcatcac atgaagatgc ctcttaatcc ctagtctagc gattggggat
     1381 ttttattttc caaaaaatga tgaaaaagct ttgcaattca tggaaaaagt agtataatac
     1441 ttctattata gaaattttta gaaaattccg aaagaggtta tttatgggat atacagttgc
     1501 tgtagtcggc gcgacaggtg ctgtcggtgc tcagatgata aaaatgttgg aagaatcaac
     1561 acttccaatc gataaaatcc gttaccttgc ttctgcacgt tcagcaggta agtcattgaa
     1621 atttaaagat caagatatta cgattgaaga aacgactgaa acagcttttg aaggagttga
     1681 tattgctctc ttttcagcag gtggttcgac atcagctaag tatgcaccat acgcagtaaa
     1741 agctggcgtg gtagtagtgg ataatacatc ttatttccgt caaaatccag atgttccttt
     1801 ggttgttcca gaggtcaatg ctcatgcact tgatgcccac aacggaatca ttgcctgccc
     1861 taactgttca acaatccaaa tgatggtggc tcttgagccg gttcgccaaa aatggggctt
     1921 ggaccgtatc attgtttcaa cttatcaagc cgtttcaggt gctggtatgg gagcaattct
     1981 tgagacacaa cgtgaacttc gtgaggtctt gaatgatgga gtgaaaccac gtgatttgca
     2041 tgcggaaatc ttaccttcag gcggtgacaa gaaacattat cctatcgcct tcaatgctct
     2101 tccacaaatc gatgtcttca ctgacaatga ttacacttac gaagagatga agatgaccaa
     2161 ggaaactaag aaaattatgg aagatgatag cattgcagta tctgcaacat gtgtgcgtat
     2221 tccagtcttg tcagctcact ctgagtctgt ttatatcgaa acaaaagaag tggctccaat
     2281 cgaagaagta aaagcagcta tcgcagcctt cccaggtgct gttcttgaag atgatgtagc
     2341 tcatcaaatc tatcctcaag ccatcaatgc agttggttcg cgtgatacct ttgttggtcg
     2401 tatccgtaaa gacttggatg cagaaaaagg aattcacatg tgggttgttt cagataacct
     2461 tctcaaaggt gctgcttgga actcagttca gattgctgaa actcttcatg aacgtggatt
     2521 ggttcgtcca acagccgaat tgaaatttga attaaaatag tcatatcgtt taggagttca
     2581 gatgaactcc ttctttgaaa tagagaggtg ttttcgtgtc ttatcaagat ttaaaagaat
     2641 gtaaaatcat tacagccttt attaccccct tccatgagga tggttccatt aactttgatg
     2701 ctattccagc cttgattgag catttattgg cccatcatac ggatggaatt cttctcgcag
     2761 gaacgactgc tgagagtcca actttgaccc acgatgagga gttggagttg tttgcggctg
     2821 tacaaaaggt tgtcaatgga cgcgttcctt tgattgcggg tgtaggtact aatgatacgc
     2881 gtgactctat tgagtttgtc aaagaagtag cggaatttgg tggtttcgca gctgggcttg
     2941 ctattgttcc ttactacaac aaaccttctc aagaagggat gtatcagcac tttaaggcta
     3001 ttgcagatgc ttctgaccta ccaattatta tctataacat tccagggcgt gtagttgtcg
     3061 aattgactcc agaaaccatg cttcgcttgg ctgactatcc aaatattatc ggtgtcaaag
     3121 aatgtactag cttggctaat atggcttact tgattgagca caagcctgaa gagttcttga
     3181 tttatacagg tgaggatgga gatgctttcc atgccatgaa ccttggggcg gatggggtta
     3241 tttctgttgc ctctcataca aatggggatg aaatgcacga gatgtttact gccattgcag
     3301 aaagcgatat gaagaaagcc gcagcaattc agcgtaaatt cattcctaag gttaatgctc
     3361 tcttctctta tccaagtcct gctccagtta aggcagttct taactatatg ggatttgaag
     3421 ctggacccac tcgtctacct cttattccag caccagaaga agatgccaaa cgcattatca
     3481 aggttgtcgt agatggcgac tacgaagcaa ctaaggcaac tgtaacaggg gtcttaagac
     3541 cagattacta ataaagacaa taaaatccat gaccgaatga aggatcatgg atttttctta
     3601 ttttcctaaa cagaattggc taaagagttg ggtgatgagt tcatctggag cagcatctcc
     3661 agtgatttct cctagaattt cccaagtacg ggtcaagtca acttgaagca agtcaactgg
     3721 catccctagt tcaagacctt ggttaacagc ttgtaggctt tcaacggcct tctcaatcaa
     3781 ggaaatgtga cgggcgtttg acaagtaggt agcatcttgc tcaaccaaac cagcattttc
     3841 aaagaagagg ttgttgattc gctcttcaat cttatcgatg ttttggtttt taaggactga
     3901 aatacggata acgtcttcag gtagtttcga agtttcaatc gtttctggca ggtcggtttt
     3961 attaagtaga ataatgcgat tggtatcttg gctaatttca agaagttgtc tgtcttgcgc
     4021 agtcagtggt tcactggcat ttagcactag tagaaccaag tcggcttcct tgagggcttt
     4081 tttcgaacgc tcaacaccga tttgttccac tatgtcatct gtttcacgaa taccggctgt
     4141 atcaatcaat ttgagaggta caccattgat gttgacgtac tcttcgatga catctcgtgt
     4201 tgtcccagcg atatctgtta cgatagcctt gtcctcacgc aagaggttgt tgagaaggct
     4261 tgatttccca acgttgggac gtccaatgat agccgttgaa attccttcac gaaggatttt
     4321 accacgacgt gctgtcctaa ggagcttggt tagtaattgc tcaaactcca ttgtcttctc
     4381 tcggacaaca gcagtagtgg cttcctcaac atcgtcatac tcaggatagt cgatattgac
     4441 ctcaacttgg gcaagtgtgt taaggatttc ttgacggata ttattgataa gatcagaaag
     4501 ggaaccatca agttgtttga ccgcgatgtt catggccttg tctgtcttgg ctcggatgat
     4561 atccatcacc gcctcggcct gtgtcaaatc tacgcgaccg tttaaaaagg cacgtttggt
     4621 aaattcacca ggttctgcca accgagctcc ttcacggata gctagctgga gaatctcatt
     4681 ggtcaccgca ataccaccgt gggtgttaat ctcgataata tcctcacgag tgaaggtctt
     4741 tggagacttc atagccccaa ccataacctc gtccatgact ttaccagtca gaggatcaat
     4801 aatgtgaccg tagttgagag tgtggctggc aaccttgttc aagtcttttc ctttaaaaat
     4861 cttttgcgca atagcaaaac tgtctgttcc gctcaggcgg acaataccaa tagctccttc
     4921 acctagtgga gtagagatag cagcgatggt atcaaattca cgtgtaatca taatatttcc
     4981 tttaattaat tgctcttttc ttggaatttt ccaagtctct tttcaaattg taacaaattt
     5041 agactatttc ttcaatgggt gctacttgga gattgaaaaa gcctaagcaa ttctgcttaa
     5101 gctagcttat ttggtgcgca tttccccctg tgggaaataa gttccttctg gcatgtcgtt
     5161 gatgatgaca tgaacagcag attgaggggc tccagtgttg cgaacaaccg cttccgttac
     5221 ttccttagca agagctttct tttgctcgag cgtgcgtcct tcaaataaat cgatgcgtac
     5281 aaatggcata atagcttcct ccactagttt ttgatttctt ctattttacc acattttgcc
     5341 gtttaaagct taagaaaatt atgatatact agaatgtagc aaaaatttag aaatggacgt
     5401 gaagcaagaa acatggcaca gttgtattat cgttatggga ccatgaactc tggtaaaacg
     5461 attgagattc tcaaagtggc ctataactac gaggagcaag gaaaaggtgt tgtcattatg
     5521 acttcggctc tggatacgcg tgacggtgtt ggctatgtgt cgagtcgaat tggcatgaaa
     5581 cgccctgccc ttgcgattga ggaaacaacg gatatctttg gctatatccg agacctacca
     5641 gaaaaacctt actgtgtttt ggtcgatgaa gcccagtttc tcaaacgcca ccatgtttac
     5701 gacctagctc gtgttgtgga cgagttagac atacccgtca tggcttttgg cttgaaaaat
     5761 gactttcgca atgaattgtt cgaaggttcc aaatatctct tgcttttagc agacaagatt
     5821 gacgaaatca agaccatatg tcagtattgc aagaaaaagg cgaccatggt gttgcgaaca
     5881 caggatggac tgcccgttta cgatggagaa cagatccaga ttggtggtaa tgaaacctat
     5941 atctcggttt gccgtaaaca ttattttgcc cctgaaatca ataaggagaa tgaagaaaaa
     6001 tgaacatcta tgatcaacta caagctgtag aagaccgtta tgaagaacta ggagaattgc
     6061 tgagtgaccc tgatgtcgtt tcagacacca agcgttttat ggagctttca aaagaagaag
     6121 cttccaatcg tgacaccgta atagcctacc gtgagtataa acaagtcctt caaaatatcg
     6181 tcgatgccga agagatgatt aaggaatcag gcggagatgc ggacttggaa gaaatggcca
     6241 agcaagaact caaagatgcc aaggctgaaa aagaagaata tgaagaaaaa ctgaaaattt
     6301 tgctccttcc aaaggatcca aacgatgaca agaatatcat ccttgaaatc cgtggagcag
     6361 ctggtggaga cgaagcggca cttttcgctg gagatttgct aactatgtac caaaagtatg
     6421 cggaagccca aggttggcgc tttgaagtca tggaagcctc tatgaatggt gtcggtggtt
     6481 ttaaagaagt ggttgctatg gtttcaggtc agtctgtata ctctaagctt aagtatgaat
     6541 caggtgccca ccgtgtgcaa cgtgttcctg tgacagaaag ccaaggccgt gttcatactt
     6601 cgacagcgac agttcttgtt atgctagaag ttgaagaggt tgaatacgac attgatccaa
     6661 aagaccttcg tgtcgacatc tatcacgcct ctggtgctgg tggacagaac gtcaataagg
     6721 ttgcgactgc cgttcgtatc gttcacttgc caaccaatat caaggttgag atgcaggaag
     6781 aacgtaccca gcagaagaac cgcgagaagg ctatgaagat tatccgtgca cgcgtcgctg
     6841 accactttgc tcagattgct caggatgaac aagacgctga gcgtaagtcg acaatcggta
     6901 ctggtgaccg ttcagaacgg atccgaactt ataacttccc acaaaaccgt gtcacagacc
     6961 accgtatcgg cttgaccctc caaaaactag atacgatttt gtctggtaaa ttggacgaag
     7021 ttgtggatgc cttggtgctt tatgaccaaa cacaaaaact agaagaatta aacaaataat
     7081 gaaattagct caattatttt caaattttga agaagagttg ataagacaag gagaggaagc
     7141 ggaaagcctc tcttttgtct atcgtagtct gaaaaatcta tcttttacag actttatttt
     7201 tgccctccag caggaggtga caacagagga agaaaaacaa tttgtagaag atatttacca
     7261 gcaactagca gctcacaagc cagctcagta catcattgga caggcagatt tttatggaat
     7321 gcatttaaag gtggatgaac gggttttgat tcctcgtcca gaaacagagg agttagtgga
     7381 gcttattcta actgaaaatc ttgagacgaa tctctcagtt ctggatattg gaacaggtag
     7441 tggagctatt gctctcgctc tagcaaaaaa tagaccagat tggtcagtga cagcagcaga
     7501 tgtttctcaa gaggccttag agcttgcatc agagaatgct agcgatcaaa atcttaatat
     7561 attttttaaa aaatctgatt gttttgcaga aatttctgaa aaatatgata taattgtatc
     7621 caatccaccc tatatctctc gtgaagatga gtcagaggta ggcttgaatg ttttgcattc
     7681 ggagcctcat ctagctctct ttgcagacga ggatggccta gctatttact gtagaattgc
     7741 ggaagatgca aaagactatc tcaaggatgg tggtaagatt taccttgaaa ttggatacaa
     7801 gcaaggtcaa agtgttcccg aactttttag aaaacatctt cctgaaaaaa aagttcgaac
     7861 actcaaagac caatttggtc aaaataggat ggttgtggtt gatgatggac aggattagac
     7921 aagagttgga aaatggtgga gctgtcgttc tacctacaga gactgtttat ggtctttttg
     7981 ccaaggcctt agatgaaaaa gcagttgacc atgtttacca actcaaacgt cgtcctagag
     8041 ataaggcact caatctcaat atcgcctctt tcgaggatat cttgcacttt tctaagaatc
     8101 agccaactta tctacaaaaa cttgtagaga cctttttgcc aggtcccttg accattattc
     8161 tcgaagccaa tgaccgagtt ccctattggg taaattctga ccttgcaact attggatttc
     8221 ggatgcccag tcaccctatc acactggatt taattcgaga gacaggtccc ttgattgggc
     8281 cgtctgccaa tatctcaggt caggcaagtg gtgtaacctt tgaacaaatt ctgaaggatt
     8341 ttgaccaaga ggttctgggt ctggaagacg atgcttttct aactggacag gattcaacta
     8401 ttgtggattt gtctggagac aaggtgaaaa tcttacgcca aggcgcaatt aaacgagaag
     8461 atattcttgc tcggttgcca gagatttctt ttgaggaggc ttgaaatgct aagagatttg
     8521 caagaaacag atgtgaaagc gatatgtgac atcaaccaag aggctttggg ttatactttt
     8581 agtccagagg aaacggctag ccaactagct agactgtctc aggattccca tcatttccta
     8641 cttggctatg aggatgcagc taatcatgtc ttacttggat atgtccacgc tgaagtttac
     8701 gaatcactct attccaaagc aggatttaat atcttagctt tagcagtttc acctcaagcg
     8761 caaggtcaag gtattggtaa aagtttacta caagggttgg aagaagaagc aaaaagatgt
     8821 ggttatgggt ttatccgctt aaattctgcc aatcatcgtc tgggtgctca tgcattttat
     8881 gaaaaagttg gctatacttg tgataaaatg cagaaacggt ttattcgcat cttttagttt
     8941 gattttctta ttgtaaaatc aaactaatgg actagtcaca caataaagga gaagacctat
     9001 gatttttgac aaagatgatt ttaaagcata tgatgctgat ctctggaatg ctattgccaa
     9061 agaagaagaa cgccaacaaa ataatatcga gttaattgct tcggaaaacg tagtttccaa
     9121 ggctgttatg gcagctcaag ggtctatctt gacaaataaa tatgccgagg gttacccagg
     9181 acgccgttat tatggtggaa ctgatgtagt agacgttgta gagactcttg ctattgaacg
     9241 cgcaaaagaa attttcggtg ctaaatttgc caatgtccaa ccacattcag gaagccaagc
     9301 taactgtgcg gcttacatgt ccttgattga gccaggtgat acggttatgg gaatggattt
     9361 ggcatcaggt ggtcatttga ctcatggggc tcctgttagc ttctctggtc aaacctacaa
     9421 ctttgtttct tatagtgttg atcctgaaac ggaactctta gactttgatg ctatcttgaa
     9481 acaagcccaa gaagtaaaac caaaactgat tgtagctggt gcttcagcat attctcaaat
     9541 tatcgatttt tcaaaattcc gtgaaatcgc agatgctgtc ggtgcgaagc tcatggtgga
     9601 catggcccat atcgctggtt tggttgcggc tggccttcat ccaagcccag ttccatacgc
     9661 tcatatcaca acaacaacga cccacaaaac ccttcgtgga cctcgtggtg gtttgatttt
     9721 gaccaatgac gaagaacttg ctaaaaaaat caattcagct attttcccag gtattcaggg
     9781 cggtccttta gagcatgttg tggcggctaa ggcagtttcc ttcaaagaag ttttggatcc
     9841 agccttcaag gaatatgctg ccaatgtaat taagaacagc aaggctatgg cagatgtctt
     9901 cttgcaagac cctgatttcc gtattatttc aggtggaact gaaaaccatc tcttccttgt
     9961 tgatgtgact aaggttgtag aaaacggaaa agttgctcaa aacttgctgg atgaagtcaa
    10021 tattacccta aataaaaatt caatccctta cgaaaccttg tcgccattca agacaagtgg
    10081 gattcgtatc ggagcagcag ctattactgc acgtggattt ggtgaagaag aaagtcgcaa
    10141 agtggctgaa ctcatcatta aaacccttaa gaattcagaa aatgaggctg tattagaaga
    10201 agtgagaagt gcagtcaaag aattgacaga tgccttccca ttatacgagg actaaaactt
    10261 ttatggatat ttatattaag aaagccatta ttcaccagtt cagtccggat gataccgagc
    10321 tgttcttagc agataagttt ctcaatatta ctccaaaaat cgaagaatac ctacgtaaaa
    10381 aaattgaaca tgtgtattca gatgaagcca agactgggat tttcgaagaa gaaaatccct
    10441 tcttcaatca tattacagac gatttgttgg agacatcagt aacgctggct aatctctgga
    10501 aagaggagtt tagcatttct gaaaatctca agaccaatga cttgattttt gttcaatttt
    10561 ctaaagaagg tgtagaacat ttcgctttct tgcgaattgc cctgcgggag accttgaccc
    10621 acctcggagg agaagttgat aatccaatca agctaactca gaataacctg cctggatttg
    10681 gaacaggagc tgatgaggcc ttggtggtca atcttcagag tagcaagtat cacctgattg
    10741 aaaaacgaat caagtacaac gggacttttt tgaactattt ttcagataat cttcttgctg
    10801 tcgctcctaa gatttctcct aaaaaatcta tcaaggaact ggaaaaaaca gcccagagaa
    10861 ttgctgaatc ttttaacaca gatgattttc aatttcaatc caaggtcaaa tcagctattt
    10921 tcaacaacct agaagaaagc aatgaattgt cacctgagaa attggctaat gacctttttg
    10981 acaacaatct gacggctcgt ttgagcttta ttgaccaagt caaagaagcc gtaccagaac
    11041 ctgttcaatt tgatgaaatt gatgccagtc gccaattaaa gaaatttgaa aaccaaaaac
    11101 tctccttatc aaatggaatt gagctcatcg ttcccaataa cgtctatcaa gacgccgagt
    11161 ctgttgagtt tatccaaaac gaaaatggaa cctactctat cttaatcaaa aatatcgagg
    11221 atatccaaag taaataatgt ttaaacgaat tcgaagagtg cttgtactag cagtcttcct
    11281 ttttgctggc tataaagctt accgcgttca tcaagatgtc aaacaagtca tgacctatca
    11341 acccatggtg cgagaaatat tgagtgaaaa agacacccca gcaaacgaag agcttgtgct
    11401 tgctatgatt tatactgaaa caaaaggaaa agaaggcgat gttatgcagt ctagtgagtc
    11461 tgcaagtggt tccaccaaca ccatcaatga taatgcctct agcattcggc aaggcattca
    11521 aactctgaca ggcaatctct atctggcgca gaagaagggt gtagatatct ggacagctgt
    11581 tcaagcctat aattttggac ctgcctatat cgattttatc gcccaaaatg gcaaggaaaa
    11641 taccttggct ctagccaaac agtactctcg tgagactgtt gcccccttgc ttggtaatac
    11701 gactggaaag acttatagtt atattcaccc catttccatt tttcacggtg ctgaactcta
    11761 tgtaaatgga ggaaactatt attattctag acaggtacga cttaaccttt acatcatcaa
    11821 atgtttcact ctcttttcaa catctggcta gaccaggtgt ttttgttata agttttcttt
    11881 aagatagata tattactata aagaagtaaa ggaggaaatt ccctatgaga aagaaactct
    11941 ttctgactag tgctgcggtc ttgtgggcag taacagctat gaatagcgtc catgcagcaa
    12001 cagatgttca aaaagttatc gatgaaacct atgtccaacc tgaatatgtc ctaggttcct
    12061 ccctatctga agaccaaaaa aatcaaactc ttaaaaaact gggctacaat gcctcaacag
    12121 ataccaaaga actcaagacc atgacacctg atgtttattc taaaatcatg aatgtggcca
    12181 atgactctag cttacagttg tattcatcag ccaagattca aaagctaggt gacaaatcgc
    12241 cacttgaggt caagattgaa acaccagaaa atatcactaa ggtgactcag gatatgtacc
    12301 gaaacgcagc agtaacgctg ggtatggaac atgccaaaat cactgtagca gcccctattc
    12361 cagttacagg agaaagtgct ttagcaggga tttactattc gctagaggct aatggagcca
    12421 aggtgccaca agctaataaa gatttggctc aagaagagct aaaagctttg tcagatatca
    12481 atgctgaaaa caaggacaaa tcaggctatg atgctaataa attaaacgtt gccctagctg
    12541 atatcaagtc aggactcgcc aaagctaaag aaagcaaggg aaatctgaca gaagaagata
    12601 tccgcaagat tgttgaagat accttaaaaa attacaaact tgatcaggtc ataacaggaa
    12661 accagatcaa tatcatcatc aattttgcct tgaatctctc aaagagtgat atcctcagca
    12721 atgcagattt cactaaaacc ctaaatgacc ttaaacaaag catcgtatca caagctggcg
    12781 acagttttaa aaatatcaac cttaactttg atgcggataa ggcgctagag gacggtggta
    12841 acttcttaag ctccctctgg caagcccttg tcaacttctt caagagtttt ggttcttaag
    12901 aaatttcatg gtataatgga tggtaaccgt aacgttgccg tcttttttgt cggccaatag
    12961 aaagagaaaa gaatgttaaa gaaaaatgat attgtagaag ttgaaattgt tgacttgacc
    13021 catgaagggg caggagttgc caaggtagat ggtttggtct tttttgtaga gaatgcttta
    13081 ccgagtgaaa aaattctcat gcgtgtcctc aaggtcaata aaaagattgg ctttggaaaa
    13141 gttgaaaaat accttgtcca gtcaccacac cgtaatcaag atctagattt ggcttacctg
    13201 cgttcaggaa tcgcggattt aggacacctt tcttatccag aacagctcaa gtttaaaacc
    13261 aagcaagtca aggacagtct ctacaagatt gctggaattg cagatgtaga agttgctgaa
    13321 acgcttggta tggaacatcc agtcaagtat cgcaataagg cgcaggtgcc cgttcgtcga
    13381 gtgaatggtg tcttggaaac aggatttttc cgtaagaatt cgcacgacct catgcccctt
    13441 gaagatttct ttatccagga tcctgtcatt gaccaagtcg tagtagctct tcgagacctg
    13501 ctccgtcgtt ttgatttaaa accttatgac gaaaaggaac agtctggatt gattcggaat
    13561 cttgtggtgc gtcgtggtca ctattcagga caaatcatgg tcgttttggt gacaactcgt
    13621 ccaaaagttt ttcgtgttga ccaattgatt gaacaagtta tcaagcagtt cccagagatt
    13681 gtgtctgtca tgcaaaatat caacgaccag aataccaatg cgatttttgg taaggagtgg
    13741 cgcactcttt atggtcaaga ctatattacg gaccagatgt tgggaaatga cttccaaatc
    13801 gctggcccag ccttttacca agtcaatact gaaatggcgg agaaactcta tcaaacagcc
    13861 attgactttg cagagttaaa aaaagatgac gtggttattg atgcttattc tggtattgga
    13921 accattggtt tatcagtcgc caagcatgtc aaagaagtct acggtgttga actgattcca
    13981 gaagcggttg agaatagtaa aaaaaatgct cagctgaaca atatttcaaa cgcccactat
    14041 gtctgtgaca cagctgaaaa tgctatgaag aattggctta aagatgggat tcaaccaacc
    14101 gttatcttgg ttgatcctcc acgcaagggc ttgacagaaa gctttatcaa agcaagcgcc
    14161 caaacaggag ccgatcgcat cgcctatatc tcctgcaatg tcgcaaccat ggcgcgtgat
    14221 atcaaactct accaagaatt gggatatgaa ttgaagaaag tccagccggt ggatctattt
    14281 cctcaaacgc atcacgtcga gacggtagca cttttgtcca aactcgatgt cgataagcac
    14341 ataagtgttg aaattgagct ggatgagatg aatttgacaa gtgcggagag caaagcaaca
    14401 tatgctcaaa tcaaagaata tgtttggaat aaatttgaat taaaagtttc gacattatat
    14461 attgcacaga taaaaaagaa atgtggaata gaattacgag aacattacaa caagtctaaa
    14521 aaggataaac aaattattcc acagtgtaca cctgaaaaag aagaagccat catggatgct
    14581 ttgagacact tcaaaatgat ttaatagaaa agaatgacag tatatgactt tctgcattta
    14641 ttacattcct acttggtata ggaacagcta ttattccttt cttgcaaggt atcaattaga
    14701 aaatagg
//
LOCUS       DAVHIU010000043        14212 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVHIU010000043 DAVHIU010000000
VERSION     DAVHIU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.045"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..538)
                     /locus_tag="VVN06_001114"
     CDS             complement(<1..538)
                     /locus_tag="VVN06_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562672.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFIN"
     gene            complement(610..1170)
                     /gene="rnmV"
                     /locus_tag="VVN06_001115"
     CDS             complement(610..1170)
                     /gene="rnmV"
                     /locus_tag="VVN06_001115"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU5562673.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1170..1943)
                     /locus_tag="VVN06_001116"
     CDS             complement(1170..1943)
                     /locus_tag="VVN06_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5562674.1"
                     /translation="MIFDTHTHLNVEEFAGRETEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2248..3141)
                     /locus_tag="VVN06_001117"
     CDS             complement(2248..3141)
                     /locus_tag="VVN06_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU5562675.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(3425..3559)
                     /gene="rpmH"
                     /locus_tag="VVN06_001118"
     CDS             complement(3425..3559)
                     /gene="rpmH"
                     /locus_tag="VVN06_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5562676.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3704..4918)
                     /locus_tag="VVN06_001119"
     CDS             complement(3704..4918)
                     /locus_tag="VVN06_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5562677.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLASDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5074..5366)
                     /locus_tag="VVN06_001120"
                     /pseudo
     CDS             complement(5074..5366)
                     /locus_tag="VVN06_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5528..5980
                     /locus_tag="VVN06_001121"
     CDS             5528..5980
                     /locus_tag="VVN06_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5562678.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6015..7403)
                     /locus_tag="VVN06_001122"
     CDS             complement(6015..7403)
                     /locus_tag="VVN06_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5562679.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQTLDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            7469..8431
                     /locus_tag="VVN06_001123"
     CDS             7469..8431
                     /locus_tag="VVN06_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5562680.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(8460..8708)
                     /locus_tag="VVN06_001124"
     CDS             complement(8460..8708)
                     /locus_tag="VVN06_001124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562681.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSSKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(8772..9782)
                     /gene="ccpA"
                     /locus_tag="VVN06_001125"
     CDS             complement(8772..9782)
                     /gene="ccpA"
                     /locus_tag="VVN06_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU5562682.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10207..10806)
                     /locus_tag="VVN06_001126"
     CDS             complement(10207..10806)
                     /locus_tag="VVN06_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5562683.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(10808..11905)
                     /locus_tag="VVN06_001127"
     CDS             complement(10808..11905)
                     /locus_tag="VVN06_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5562684.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNILIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKIDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(11905..12642)
                     /locus_tag="VVN06_001128"
     CDS             complement(11905..12642)
                     /locus_tag="VVN06_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5562685.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(12644..13528)
                     /locus_tag="VVN06_001129"
     CDS             complement(12644..13528)
                     /locus_tag="VVN06_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562686.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(13515..13709)
                     /locus_tag="VVN06_001130"
     CDS             complement(13515..13709)
                     /locus_tag="VVN06_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562687.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(13706..13912)
                     /locus_tag="VVN06_001131"
     CDS             complement(13706..13912)
                     /locus_tag="VVN06_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562688.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         4170 a         3283 c         2548 g         4211 t
ORIGIN      
        1 gatttataaa tttggcaaga tcttttctaa tgagcatttg ataaaaaaac aaacgaatca
       61 acaacatatc atttgttgaa taaacttctt tttctatgat ctgaggcata tgttcctcaa
      121 gtattaactc tgcatattca ggccttctag ttctagaagt atccaaacac gcctgcaata
      181 cttcacatgc taattgttct tctttaggaa gattatcaaa gtatgtttta taaatctctt
      241 ccaaacacgc ttccttctta tcgtactcct cttctttacc atagattggt tcacgtaaaa
      301 tctgatattt caattctaaa taagcaggag gaagagctga aaaatccggc ataaggctat
      361 aaactggaac tcctagccgg cgagcaatat agtctaactt gggcaaactc ggttgcgaag
      421 ctccactttc aattcgcgac agttgacgaa ctgtcagttc ttgctcatct ccacaaaaat
      481 ctggacgact aattcctttt tctatccgaa tctctttaat tttttgacca attaacatca
      541 gacgcctcct cttctacctg cttcgaaaaa tactgtgaaa cttaaaaaat tgtaacggct
      601 acatctttcc tactcataag atttcatagc ttcttccact tctgccaaag taaccccaaa
      661 caactctagg cgtttgagga gttgcttgcc gttagaatag ccgattcgga gagtctctcc
      721 gagatattct ctacgcttac ggctgtctgc ccctgctaga aaaccaaggc gaatcaagtc
      781 gctacgacta atgtcaaact gactctcatg ttcaaattgt tctgtcacct gagctagagc
      841 cgttttcagg tcttcatagc tggcatgctc aattcccaga gaacgcccct tggtcttgga
      901 cttgggaaca gcttcatctc gcttgagaaa ggcatgctga actgttggaa tgaccgtcat
      961 gatcatgcgc cgaatccgtt ccccattaaa atctgggtct gtaaagacaa tgactccatg
     1021 acgttggtgc aggcgctgaa tccgctctat atcctgatca ttgatggcag aacctcgagt
     1081 ctcataggtc tccacatcga aataacgttt gagattgacc gtatcatcgc gaccttcaac
     1141 cacgataact tgagaaattt tctctttcat tacttgctgt ccaatccaaa aattcgttct
     1201 gcatttgcag tcgttgctac cgccagctct tctgtcgtca taccacgcaa gtcagcgata
     1261 aagtcgacca catagcgagt ataggctgtt ttattttcac gaccacgctt gggtacaggt
     1321 gctaagtaag gcgcatctgt ttccaccaac atcttgtcca aaggtaactc tttagctgct
     1381 tcttggaggt cagttgcctt cttgaaagtc accactcctg agaaggaaat ggtcatacca
     1441 agatccacaa acttctctgc ccactcaagc gtccctgaaa atgaatgcat gataccacca
     1501 cgaggaccaa cgccctcact cttgataatc tcataggtat cttccagcgc atcacgggta
     1561 tggacaacaa aaggcaaatc caagtcctta gatagctgaa tctgacggcg aaaaacctgc
     1621 tcctgcacct ctttgggcgc tgtcatccaa tggtagtcta agccaatttc acctaaagcc
     1681 acaaccttgg aatgttttaa cttatccaac aagtaagcct caacttcctc tgtataagta
     1741 ccagcttctg taggatgcca accaatagtc gcatagagct gctcatactc atctaccaac
     1801 tccaaggcat gctcaatcgt cggtttatca aaaccaacaa tattcatctg tgtcacaccc
     1861 atctcagcag ccaaggcaat ttcttctgtc tcacgacctg caaattcttc tacattcaag
     1921 tgtgtatgtg tatcaaaaat catctcttct aacctcgttt tctatcttct attataccaa
     1981 aaaagaggag gggcacctaa tttttcggtt tcccctcctc tcttcaatag agagctattc
     2041 tgctatcttt tctatccgat attgcccatc tcctattcca cagttagaga cagaagagat
     2101 tggctacatt gatagaatat tcctattcag ctaaagtatg tgattatctt gggttctcac
     2161 ttttctcacc catcactagg attgatgact tctcaaaatc ccccttataa tctaacttta
     2221 agtagagaat agaattttct agctgttcta ttttctacgt ctagtctttc ttcctagcaa
     2281 agcaaaacca cttgttgcca ccactagacc aagactagca agtgctgcac ttgatgctga
     2341 acctgtgtta ggtaattgtt tgccagcagg tgactggtag gtaacatctt gcattgttcc
     2401 tgcagttgct tgcggtgcac ttgttgtctt aggagcagca ttaggagcca ctggtctctt
     2461 aggaactgta accatggcaa tggcttgcac tccaatcgtt tctgcttcct tcacatcttc
     2521 catagttttc gcattttcaa tctctttgag agctgcttgt ttttctgctt ccactcttgc
     2581 taaaagttct gctttttctt tatcagaaag cggtgcgcct ttgatttcat cctgcttgtt
     2641 cttggctgct ttttcaatgg catttttagc tgattctttt tcagtagcta attgcttaga
     2701 agcttgtgat tgagctgagt tttgtgaacc tgtgtgagct gatccacccg cctggctacc
     2761 accgccttca ttcgcattag catctgagct tccagtattg cctcccttat ctccacctgg
     2821 tgtagtttct agcttatact ctggtgtagc ttcttgagtt acagattcac ctttcgcaga
     2881 ttgttgaact gtatcttttc ctagaatcgt cactactgaa ccatctggga atgtgattgt
     2941 tgctgtacca tctccagcta cattgattgt cgctccgtct aatgctgaac catttgcttg
     3001 taaaatagca accttaactt tttcttcatc tgttaaatga gttatatctt ttacatctac
     3061 tctttgagca ggtgctttaa cagttgattt ttcagcagtc ggtttagtgc tatcttgtgc
     3121 ttttggatct tgtgctacca attgatttcc tggaattgta atagctgaac catctttaaa
     3181 gacgattgtc gctgtgccat ctccagctac ttcaatttta tagtaaaata aaataataac
     3241 aggacaaatc aatcaggaca atcaaatcga tttctaacaa tgttttagaa acagaggtgt
     3301 actattctag tttcaatcta ctgtaacatc gcttccgaat ccatttgcat ctactgaact
     3361 tttttacaaa aataaaaatc agtagactcg agttcctact gatttttgat agaattcgtt
     3421 tggattatgc agccaaaact ttgcgtcctt tacgacgacg agctgccaat acgcgacgac
     3481 catttttagt tgacatacgg ttacggaatc cgtgtttgcg cgcacgacga agtttacttg
     3541 gttgataagt acgtttcacg atgaatacct cctcatagat tttgtattcg tttagccggc
     3601 tataaagtga tcagttacta aacatacttt actattctat ctgattcttt cacttttgtc
     3661 aatagctcta ggcaaatgtt tccagctttt tcgaatgcaa gccctatcta cgatactgtt
     3721 tcaagaaacg agtcatcttt tcttggattt gggctaactc atcaacacga ggaaggtaaa
     3781 cgatacggaa gtggtctggt tcctgccagt taaagcctcg accatgaacc aagagaacct
     3841 tttcctgctt caagaaatca aggacaaact gctcatcatc atcgatacgg tacatattgc
     3901 ggtcgatttt tgggaagata tagagccccg ccttgggttt aacggcagac aaacctggaa
     3961 tatcttgaat ggcattatag atgaaatttc tttgctcgta gattcgtcca ccaggaagaa
     4021 gcaattcatc gactgattgg tgacccccca aggaagtttg tacgacttgt tgggccaaaa
     4081 cgttagagca aaggcgcata ttggacagca tattgagccc ttcgatatag cccttaacat
     4141 gagtcttagg gccagacaag accatccaac ccacacggaa acctgctatg cggtgggatt
     4201 ttgacagacc attcatgctg acacagaaga catctgatgc caagctcgcc acaggcgtat
     4261 gcacatgtcc gtccattacc atgcggtcat agatttcatc cgcaaagata atcaaatcgt
     4321 tttgacgggc aatctcaata atctccagca agagttcctt aggataaagg gctccagttg
     4381 ggttatttgg attgataagg acaattgcct tggtattgga agtgattttt gacttgatat
     4441 cgtcaatatc tgggtaccat tccacagctt catcacagat atagtgaacg gcatttcctc
     4501 cagctaggct gacagccgcc gtccatagag gatagtctgg cattggcact aagacctcat
     4561 ccccattgtc caaaagcccc tgcatggaca tgacaatcaa ctcactgaca ccatttccaa
     4621 ggtaaatatc atcaatatct acgtttggga atttcttcag ctggcaatac tgcatgatgg
     4681 ccttacgggc tgagaaaatc cctttggagt tagaatagcc ttcactatca cgcgcattca
     4741 taatcaagtc atgaatgacc tcgtctggcg ctgtaaagcc aaattctgct ggatttcctg
     4801 tattcagacg taaaatcttt tctccattcg ctcgcatccg catggcttct tccaatacag
     4861 gaccacggat atcataagca acatgctcta acttactaga cttgttatat tctttcatct
     4921 tgtttcctca attcatcagg atagtaacat tataccacta gaaagagaat gcgacaagtt
     4981 tactgaaatg tgttactagt ttcgtataga agaaaaacct tgctaaaggc gaggttaaga
     5041 acgtagagat agcaggtatt ttgcaaagac acatcacata ccaaaagata aacctagaaa
     5101 aagcaagcct agaaaggagt cataaagaag attaaacatg aggaaaagcc ccatatcatc
     5161 aagtaatccc aacgcccact tcgttttgca actagaaata agattagatg gtagagtaca
     5221 tgaaagacta aaaatccaat aaaacctgga taaagaagcg gtaccagact ctctaattga
     5281 cagatcatga tcacttctac caagactgaa actaggataa ttccataata caggaaattt
     5341 gatttttgaa aagagttttt catcattctc ccttccttca cttctttcct gctatccttt
     5401 cttttatttt atcatatatt tatatagagt ttaagcccta atattctttt gaaaaatact
     5461 ttttttgctt tacttctata gtgaaaaggt ttacaattat agtaagaaaa tttcaggagg
     5521 tttcattatg gcacaacgtt accaaaatat catggtcgca atcgatggtt ctaaggaagc
     5581 ggacttggct tttgtcaagg gagttcattc tgctctacga aacgacgcta aactcaccat
     5641 cgcacatgtc attgacacac gcgctctcca aagcgtatcc acctttgatg ctgaagttta
     5701 cgaagaactc caagtcgacg ctgaaagtct gatgaaagag tacgaaaaac gtgctaaaga
     5761 tgctggggta gcagatgttc atatcgtcat tgaaatggga aatccaaaga ccctgctagc
     5821 acgtactatt ccagatgccg aggaagtgga cctcatcctc gttggcgcaa ctggtctcaa
     5881 cgcctttgaa cgcctcttgg tcggctcttc atctgaatat atactccgcc atgctaaggt
     5941 cgatttgctg gttgtgagag aacaagaaaa aaccttataa tcacaaagaa aaggagcccc
     6001 tagctccttt ttgtttacga tttatttctc tctttatggc gttcgtaagc cttgagctgg
     6061 cgctgcagtt cctttttaat agcaggttct ggagcatatt tttcttccca attatctggt
     6121 tttaagattt tatgggtcac tggatcaaaa tgagccttgc catctggaaa aattttcccc
     6181 atattggcct gatggacaat atcaaaaata cgttctgggt ccacccccat caagacaaaa
     6241 ctgccgtagg tgaagtaaag cgtgtcaatc aaggcatcca cttgccctat caaatcttgc
     6301 tgagcaggtg tcttcttggc tactttatct gctgccttat caagggtctg atgaagttgc
     6361 gatacagctt gaccaaaatc ttcttcagaa ggactggctg ctcgaacaaa ctccaccaat
     6421 tcttctattt taaaaccagc cctatgggtt gcaccctcta aatcccaagc tcgaggttct
     6481 tcttgggttc gttcatccat catgtggtgg aaggtcttga ccttattgaa atgatagtca
     6541 cggctgacaa agactttttc tgaagacaaa acaccaaaat gttccaaggc cttgtggatt
     6601 ccatcttgtt gattgctagt ggtaatgtgc ttggcagctt ccttgacact gctactacca
     6661 tttcccatag cgaccgacat accgacgcca gccagcattt caagatcatt atctgagtca
     6721 ccaaaagcca tgacttggtt gaggtcaaag ccatattctt tcccaactcg gcgaatgcct
     6781 tctaatttag aatttccctg attgatgaca tccgatgcaa aaggattgct acgtgtcaat
     6841 ttcaagtctt caaaatcagc tgccgccttc tcagattctt ctggtgtcat cagcatcaaa
     6901 acttggtaga taggctgatt catcaggtga agcaggtcct cttccttttg gggaacaacc
     6961 ttgctgacca tgcgattaaa agactgactc accgtccgag ttaaaacaga gggaacgaag
     7021 cgactaattc gttgggaaaa agaacccaga ccaaaggaca tgattttaga acccaacatg
     7081 gcatccttgg tccctagagc aatctccgtg ccctcttttt tagcatagct aattagatgg
     7141 cgcaaatgta acttggaaat agggctcgtg aacaagactc tgtctttact aaagatatac
     7201 tggccattat aggttaccgc aaaatccaga tccaaatcgt ccatcaattc cttaacaaaa
     7261 aaaggtcctc gccctgtcgc tacgccaact agtacccctt gttctttgac aatcttaatc
     7321 gcgtccttag tggatttcaa aacactcttg cgattgttga ccaaggttcc atcgatatca
     7381 aaaaaaacag ctttgacttc catcctattc caatctcccc ttttgtgata caatgattat
     7441 accacatttc agaaagagtg agtaaatcat gcctaagaaa atccttgttt tacatacggg
     7501 tggaactatt tccatgcagg ccgatgcttc tggcgctgtt gtgacgagtt cagataatcc
     7561 catgaaccat gtgtccaacc cacttgaagg aatccaagtc cacgccttgg acttttttaa
     7621 ccttccaagt ccccatatca aacccaaaca tatgctggtc ctctaccaga aaattaaaga
     7681 ggaagcagat aactacgatg gagtggtgat cacacacgga accgatactt tagaggaaac
     7741 agcctatttc cttgatacca tggaagttcc ccatatgcct atcgttctaa caggagccat
     7801 gcgtagctcc aatgagctcg gtagtgatgg tgtttataat tacctaagtg ctttacgagt
     7861 ggccagcgat gacagggctg ctgacaaagg agttttggtc gttatgaacg atgaaatcca
     7921 cgctgccaag tatgtcacca aaacacatac gactaatgtc aacaccttcc agactccaac
     7981 acatggcccc ctcggtctca tcatgaaaca ggaaatcctc tacttcaaaa cagctgaacc
     8041 tcgtgttcgc tttgaccttg atcacataca aggtttagtc cctatcatct cggcttatgc
     8101 tggtatgaca gatgagctga ttgatatgct ggatttagaa cacttggacg gtttgattat
     8161 ccaagccttc ggagctggta atattcccaa agaaacggct caaaaattag aaagccttct
     8221 gcaaaaagga atcccagtcg ctctggtatc acgatgcttt aacggtattg ccgagcctgt
     8281 ttatgcctac caaggtgggg gcgtacagtt gcaaaaagca ggcgttttct ttgttaaaga
     8341 actcaacgcc caaaaagccc gcttgaaact cctcatcgcc ctcaatgccg gactaacagg
     8401 acaggctttg aaagactata tggaaggcta atactcttcg aaaatctctg caaaccacgt
     8461 cacgtcgcct taccgtatgt atggttactg acttcgtcag ttctatctac aacctcaaaa
     8521 acatgttttg agctgacttc gtcagttcta tctacaacct caaaaacatg ttttgagctg
     8581 acttcgtcag ttctatctgc aacctcaaag cagtgctttg agctgacttc gtcagttcta
     8641 tctacaacct caaaacagtg tgttgcgcag cctttaacca gccgcctagt ccgctctatg
     8701 gtattcatta agtcaacatc tcttgtttaa gagcacaaaa tcaggaaatc ttctcgattc
     8761 cctgattttt tctatttacg ttttcgtgtt gagctacgtt ctgtcaaacc atgaggtaag
     8821 agaacttcac gttcttccaa ctcttcctta tgcataatct tggtcaacat acgcatacta
     8881 atggcaccaa ggtcataaag aggttgggca atcgttgtca agtttggacg ggtaaagcgt
     8941 gagatttgtg aatcatcact agtaataatt tcaaaatctt ctggcacaga aacaccctta
     9001 tcagccaaac cgttcaagac tcctgctgcc aactcatcac ctgtcacaac tgctgcagtt
     9061 gcatttgatg aaatcaaacg ctctgctaag gcgtaaccat catcatagct atatttagat
     9121 tcaaatacca aaccctcact ataagcgatt cctgcttttt tcaaggtttc cttgtagcca
     9181 actaaacgaa ccttaccatt gatgtcatcc actagcggac cgctaacgaa agcaatacgc
     9241 tcattttctt tagcaaggta actcactgca tcaattgttg cttgcttata gtcaatattg
     9301 acacttggca actggtgctc aacatcgaca gttcctgcga gaacaatcgg agtacgtgaa
     9361 cgcgaaaatt ctgagcgaat tttatctgtc aagtgataac ccatatagat aatgccatct
     9421 acctgctttg aaaagagggt attgacaaca gaaacttctt tctcgttatc ttcatcgcta
     9481 ttagctagga caatattgta cttgtacatt tctgcaatat catcaatccc cttagccaaa
     9541 ctcgaaaaat aaccattggt aatatttgga atcacgacac cgacagtggt tgtcttttta
     9601 cttgcaagac cacgcgcaac tgcatttgga cgataatcca agcgatcaat tacctctagc
     9661 acttttttac gggtattctc ttttacattt ttattgccat tgaccacacg gctgaccgtc
     9721 gccatggaaa cacctgcttc acgagcgaca tcataaatgg ttactgtatc atctgcattc
     9781 attccttttc ctgtcctttc tatctcacac attcttttac aagtagaggt actgattgaa
     9841 gctctatatc tacttacaaa agtgaagatg tgaaaatttc gttttcatat ttctacttat
     9901 tccattctat cactaattgt aaacactttc aagtgttttt tgaagattga ttgaaaaaat
     9961 ttcatagaaa acctaggttt agctccttgc taccacctta gactaaacaa aaaggaggaa
    10021 actaagccct cctaaagtta tagtaaaatg aaataagaac aggataaatc gatcaggaca
    10081 gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
    10141 ctataggtat tgttccattc actaccgtca attttagcac atagtcttca tgaaaatatt
    10201 atatcatcat aaccaaccag attcttttgc gatattagct gcctctgttc gattaccagc
    10261 atccaatttt gaaagaatat tggtgacata gtttcgaata gttccgtttg agaggtaaag
    10321 ctgatctgcg atttcttggt tggataagcc ccgggcgatc ccctttaaga cagcaatctc
    10381 ttgttctgtc aacggattgg gacgggtcat caccacttcc atcaattcag gcgaatactc
    10441 cttgcgtcct tcgaggacgg tgtgcaaggt ttgcatgagg tctgcaacgc ttcgttcctt
    10501 taatacataa gcatccactc cagccttgac cgcacgttca aaatacccag cacgcttgaa
    10561 ggtcgtcacc acaaccacct ttgtttcaag cctttctgat cgtatccact ccaagacttc
    10621 aagacctgtc ttaacaggca tttctacgtc aaggatggcg atatctacag actccttttc
    10681 taatagttgg attgcttctt gcccattctt ggcttgaagg acagactcca catctggttg
    10741 aagcgtgagt aattgacaca tggcatctcg caacatactt tgatcttctg cgactaatac
    10801 tttcatctta gtttctctcc ttataaggta gtcgaacttg cacttccgtt ggatgtttct
    10861 gactgattac acttacttct cctgaaaatg gaaaaacacg atctcggatt gtatggagct
    10921 catctccttt tagaaaagca aagccgcagc catcatcact tactgttaaa atgagttcct
    10981 tctctgtccg ttctaatttt aagtaagctt tagacgcttt ggcatgtttg atgatattgg
    11041 tcaccaactc aagcaaaatc atagaagcca ttgactccaa ttcttgagta aggctagcag
    11101 tatctagttg gttatctgtt tccacctcaa ttccagcaat ttctaacatc tttttcacag
    11161 tctctagttc ggatgtcaaa gttctagact taagattttc cacaatggtt cgaacttcac
    11221 gcattgattc tttgctaatt tgctgtattt ctcttaattc cttttccact tgtggataag
    11281 cctgcatttg aaatagctgc aaggctagat ctatcttgac actaagcata gcaaaggtat
    11341 gtcccagact atcatgcaaa tcctgaccga tacgattgcg ctcattttct gctagcaata
    11401 gatttatctg ggtattttgc ttgacctgag cttctttcaa atcctcgaca aggcgaattc
    11461 gaaccgagcc caaagtcatc aaatcgacaa aagtaagaat tacaagcata tagactagaa
    11521 actcaacttc gattctctga aaaatcaaaa gtcgccccac aacaaggact tgagcaagga
    11581 gaaatgtcca tacatgtaaa gactttaaac tacgtacgcc aaaatgataa attaagatat
    11641 tggaaaggaa aaagaaaaac cagatataat tcccagcaac aaaggcggta ttcccagcta
    11701 catagaccag catgatcccc cagaaaagcc aagataggcg ctggttctta gttattaaaa
    11761 cacccaaata cgtcactaca aatagaatat caaccaataa atgccaagca ggaagctccc
    11821 caatcactac aggtaggatg ggaaaaagca taaaaatcaa actggtccaa aacatatagt
    11881 gtatgctttt cagtctttca agcattaagc attctcctta tgaccttgaa ggtaaatagt
    11941 caaaccaaat aaaactgctg aaaaaacaag taaataaact gtggctgata gattgatgcc
    12001 accctcgttt aagaaggtct tgagcaactc catcaattga taggtcggga gacacttacc
    12061 aactgcttgc atccagtctg gaaataaaga aatgggcatc cagagtccgc ctaaaacagc
    12121 caatcctaga taaagaagat tgcccacaac agacatcagc tgactagttg gtaagagagt
    12181 caaggtcaag ccaagtgcta caaaagcaat actccccaca atcagcaaaa tcgcagctcc
    12241 aacccatttt actagaggca tgtctacacc tcttacaaaa tgtccaactg taaaaacaac
    12301 cagaattgag accaaataat caaccagcat acttgttatc tttgatagat aatattctac
    12361 catatttaca gggctatgac gcaatgtttt ctgccagttg ttcatcttat cggtatgtaa
    12421 aacagctgga aatgagaaga tagttgtcga catcatggaa aaggcagtca tggagatgag
    12481 gtaatcacgc ataaaattcg cgggttcacc tggtgtgtcc tgatagatac cagaaaaaaa
    12541 taaatagaaa gccgtcggca tccctaccga caatagataa tagattaatt gtcgtttggt
    12601 caataaaaat tctatcttac taagtgctat ccatcgtttc atcttagtta tctcccttct
    12661 gcgtctcttc aaagattgta ttcaacaaac tacgattatt aacttcgatt tcttgcatcc
    12721 tacatcctgc ttgggctaat agttcccaga aagcatctgc ttctcgagtg actacttgta
    12781 aagaatcttg ctttagggtc cagttttcaa ccaaatttga ctgctctacg acttccttgt
    12841 aagctatagg aaggataaag tgcttttcta tttcctcgct acgcatagct agaggcgtcg
    12901 tatctcgaat caactctccc ttatttaaga gcaaaatgcg gtctgcagtg tgttccactt
    12961 cctcaatata atgggacgaa tagagaatgg tgactccttg cgcttttaac tcctggacaa
    13021 tttcccaaaa acgttgacga gttgaggtat ccatggacgc agtaggctca tctaaaaaaa
    13081 caatctttgg tcgcccaatt aaggtcaaga cgaaagaaaa gagacgcttt tgaccgcctg
    13141 ataatttttc tgccaactgt tctttttgtt gcttgacaaa ctgcaatagt tgatcaattt
    13201 cctgattgct caaaggattt ggatagatac gttgaaagaa agcaatcaac tctttaactt
    13261 ttaatttctg aacgattaca ttttcttgag ggaggtaagc cctagtatag tctaacttag
    13321 aactcgtcac tggtaaacct tgaatagata cttgaccgct tgtgaccagt ttatctccaa
    13381 gcaaacaatc taagagtgtt gtctttccgg caccatttgg accaattaag gcaacacatt
    13441 caccatcgtt gatttcaaag gagatgttcc gcaaaatttc cttgtctttt attttcttac
    13501 tcaatttctc aactttaacc acagtcatga gactctcctt tcaaccactc cattcccata
    13561 gggaaaacga cgaaaatcat aaatccaaac cccaaagcac cacaaatgaa ttggcgaagc
    13621 aaggcttgat caaaccaatc agtaaacatt tccactaacc ataccaagag tgacaggccg
    13681 ataaagaaat agatgatccc tctcttcatt cttcaaactc ctttttcaca tctctgacta
    13741 atttcaaacc ttctctaaca agccaagaca tgattccaaa gccagcaaag accttccaag
    13801 gaaaatggta gaaatcctca tccaatcccg aaaacatgag ataggtcata actcctgcta
    13861 ctactaaact cactgcgata atcattttat ttctcatctc ttcttccccc atttcatact
    13921 acaattatag tcttttaaaa tcagaggagg cagaagcttc tgtcactaga aaatatgaca
    13981 aatgtcataa aaaattttgt tcaaaacaag caagatacac tatacaataa aacacaatca
    14041 gaaaaatcta aggcaacttc ctcaaaagag atatcaaacc caattcacac cataatgtaa
    14101 actaatactt atttaaaatc aaaaagagta gaaattttta tcagacaaac acatatatag
    14161 tgtattgaat ctataacagt aggccttaaa tactaaaata tttctataaa tt
//
LOCUS       DAVHIU010000044        13186 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVHIU010000044 DAVHIU010000000
VERSION     DAVHIU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.046"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(343..1509)
                     /locus_tag="VVN06_001132"
     CDS             complement(343..1509)
                     /locus_tag="VVN06_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5562689.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(1522..2261)
                     /locus_tag="VVN06_001133"
                     /pseudo
     CDS             complement(1522..2261)
                     /locus_tag="VVN06_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2283..3107)
                     /locus_tag="VVN06_001134"
     CDS             complement(2283..3107)
                     /locus_tag="VVN06_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5562690.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            complement(3245..4459)
                     /gene="thiI"
                     /locus_tag="VVN06_001135"
     CDS             complement(3245..4459)
                     /gene="thiI"
                     /locus_tag="VVN06_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU5562691.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(4468..5610)
                     /locus_tag="VVN06_001136"
     CDS             complement(4468..5610)
                     /locus_tag="VVN06_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5562692.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            5748..6176
                     /locus_tag="VVN06_001137"
     CDS             5748..6176
                     /locus_tag="VVN06_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562693.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            complement(6220..8445)
                     /locus_tag="VVN06_001138"
     CDS             complement(6220..8445)
                     /locus_tag="VVN06_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5562694.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQTELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(8609..10561)
                     /locus_tag="VVN06_001139"
     CDS             complement(8609..10561)
                     /locus_tag="VVN06_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5562695.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(10558..11469)
                     /gene="pfkB"
                     /locus_tag="VVN06_001140"
     CDS             complement(10558..11469)
                     /gene="pfkB"
                     /locus_tag="VVN06_001140"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU5562696.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(11466..12206)
                     /locus_tag="VVN06_001141"
     CDS             complement(11466..12206)
                     /locus_tag="VVN06_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5562697.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPYSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGINGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12369..12456
                     /locus_tag="VVN06_001142"
     tRNA            12369..12456
                     /locus_tag="VVN06_001142"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12403..12405,aa:Ser,seq:gga)
     gene            complement(12659..13006)
                     /gene="ssrA"
                     /locus_tag="VVN06_001143"
     tmRNA           complement(12659..13006)
                     /gene="ssrA"
                     /locus_tag="VVN06_001143"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3878 a         2976 c         2515 g         3817 t
ORIGIN      
        1 actctgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgc ggctagcttc
       61 ctagtttgct ctttgatttt cattgagtat taatagactt gtttggtatc ctgaaaacta
      121 tatcgtatag gtgtcgtcat ggatcctttt agctgattta aggatacgaa aacacaatac
      181 tatgactaaa ttgaaatgtt aaaattcaat ttctagccac ctaatcctgc ttcaaatagt
      241 tatcgaacaa gttttatgat agctaccttg ttaggatgct cttatttttt accagacaaa
      301 acaaaaggat taagggagtg aatttcctta atccgatagt tcttattctt gacgttgtcc
      361 gaaatcagca atcatctgat ccatttcttg tcgactcgga gttgtcagca tatacaggta
      421 atctccttga agttccgcga aggcagctgg catgactttt ttaaccttga actgctggct
      481 atatttggca agcaaatcct cttctgaata aacatagtga gcatttgcac ggcgagaagt
      541 agccaaacaa tactgagttt caaagtctga cgccgggaac tcctctcctg cgacaatagc
      601 tgcatagcca cgataaaggt ccaaggaatg agcaaagtta taaacatcaa tggtaaaacc
      661 acctgcaggg cggttattgt actcgatggt aatataatcg tccccctcac ggaagaactc
      721 aatatggaaa aaccgttctt tcataccaaa ttctttgaca attgcttccc catacttgcg
      781 cagtttagga tccatatcct tgagcacata ataagaattg tccatcttat aaatcatgag
      841 gtcaagcggt gtataggcgt agtcaaaggt tgttgagaag acaatctttc catccttgtc
      901 cacgagcccg tcaaaggtac agatttcgct ggaagtgaca aatttttcaa agaaataaag
      961 ggttgaatgg tcccattctt gcttgaagtg attgatatcg tcttctgtct caagtttaaa
     1021 ggttgcggct gctcccactc cattatcagg tttggcaatc attggaagac cgatttcttt
     1081 cactgcttga tcaacatctg cttccgtctt gataacagct ccaggtacca caggaacacc
     1141 tgcttttttg aaaagtttct tcatttcaga cttatatttc gtctttttga gatcctctgg
     1201 tttggcacca aaaacattga attgttctct gagtgttgcg tctagctcaa gccagtattc
     1261 attgtgagac tcgatgcggt caattggacc atgtttataa aagagaaaag caactgcacg
     1321 tttgacttca tctatgttct caagattatc aacacgaaaa tactcggtca agctattgcg
     1381 caagggctca tccaattgct cgtaagactc ttgaccaatt cccaagactg tgatgccttt
     1441 attagctagt tcgatggtaa actgttgaaa gttttgtgga tagtagggag aaataacaag
     1501 gtaattcata ggtaactcct tttataaata gagattaccg aggaaataag gcatttgttt
     1561 acgccaccat tcccagtcat gggcgacatc atgtccccat tcagcaaacc aggctggaat
     1621 ttgtttcttg tcaaaggctt ctttgagctt gtaaaaggat ggcaaaccat cttgttccca
     1681 ggctccaaga cccgtacaca gcacaatctc tgcctgacgg taacggtcaa taaaccagcc
     1741 gtcgttttgg ttccaaatat aatctactgg cgagttttgg taaatagcat catcgttgta
     1801 gtaatcaccg acaaagaaac gtgcgtcgta aacaccactg agagcaatca ctttggtaaa
     1861 gacatctgga tgttggagga agaaattgag tgcatgatag gctcccatag agcaacctgt
     1921 cgtcatcatg ccatcaaacc aacctgactt gtgcttgata aaagaatggc ctcctcaatc
     1981 acataacatt cgtaggcacg gtgcatttcc gcttggtcat gagcattttt ccaagtagcc
     2041 aaccagctct cactatccaa actagatagg gtaaagaact ggacacggcc ttcctcgata
     2101 aaggaagcac aggcatcaat catgccaaaa tcatagtatt cgttgtgact accacctgat
     2161 gaagcaaaga ccacaactgg aatcccacca tgtccataac ggttaaggta catttcacgg
     2221 ttaggatgac cactccagtg gctaagatgt tcaatatgca tttcgttttt tcctttctta
     2281 acttaccatt tttctgcaaa aaatctcaga caatctggta gattttctga ccaagggatt
     2341 tcactatgga tggcaccaga ctgaactttt agcacaagat tatccagatg tactccccct
     2401 gctatcaaat catggtaata gcaaagcgac gagtcgatat aggcttgttt gatattgcca
     2461 tccatcaagg tcttgtctgt atcatctgct tcttctgttc ctacatagat gaagatgcgc
     2521 tggtcaggcg atagtttctg gcactcgata tagcggttaa aggcttcttg gtggagccag
     2581 tttgcagatg aaaaaacgcc caagcaacca atttggtctt ggtattccaa accgataaac
     2641 tgggtaatat tgcctcctag tgaggaacca atcatagccg tatgctggca gtctgctttt
     2701 gtacgatagg tctcatcgat aaaaggcttg accacctcca tgacaaactc agcatactcc
     2761 acacccttac caccaaactg ctgctctggg ataggagatt cttggaactt ccaagccgca
     2821 tactcattca ttcgccccat accatcattg tcaatagcaa cgacaatcat gcgactgata
     2881 tccggatttc gtttgatagc tgggataatc ttccatgaat atccaatgaa agactctttg
     2941 ctataaaaaa cattttgccc gtcatgaaag tatacaacag gataggaacg gtctgtatct
     3001 ttctcataat ctttaggaag aagaatacgt acacggcgct ccttacctgt ataaggaacc
     3061 ttgagtttgt gttctttcat ttttagataa aagtaggatt gattcattgt cagaaaactc
     3121 tctatattca aaattttata tcattatatc atgaaaataa aaaaaaagtt agttttttac
     3181 ctgtttttag aaaaaaaaac taactttttt tactgatttt cgctattctt ttggattttc
     3241 tgaattagag tagattatca atcaagtcat caacttcatc tttttcggct tgaggtgtga
     3301 tttcagtaat catgattcca gccactgctc gctcaaccaa gccttcaaca tccatacgcg
     3361 cttcgtactg ctccgcattc ttaattttag gatttgtttt tggacgatct ggtgcaaaaa
     3421 tggtacaaca gtcttcaaac ggttggattg aaatgtcaaa ggtatcgatt tcctgggcga
     3481 tgtcaatgat ttccaacttg tccatggtaa ccacaggacg aatgatggga gtgttggtaa
     3541 cagcattgat agccttcata ctttcaaggg tttggctggc tacttgacct agactttccc
     3601 cattgatgat aaccaaacca tttcgtacct cacgaatacg gtcagtaatc cgcatcataa
     3661 agcgacgagt tagagtcatc aaataagctt ctggcgcttt ggctttgatt tcctcttgaa
     3721 tctctgtgaa aggcacctcg ataaactgga tatttccgcc aaacttggtc aatttacgag
     3781 tcaagtcctg cgctttcttg agggcaccag gactagtata tggtggacta gcaaagtgaa
     3841 ctgcctcgat atccacccca cgcttaagag caagataacc tgctacaggt gagtcaatcc
     3901 ctcctgacaa catgagcatc cctttacctg aagttccaac tggcaaacca ccagccccac
     3961 gaatggtttc ataagaaaga taggctgctt cttcacgaat ctccacctga agattgatgt
     4021 caggactttt catttgaact tgcacatttg gaatggcttc gaatacagcc cctccaagtg
     4081 tttggttgag ttcacgacta tcaagttcaa agttgtggtc gctacgcttg ctagaaatct
     4141 taaaggtcat accttccttg tagatgtccc gcataatctc ttggacagaa gacttcaaaa
     4201 cttctacaga tttttcaacc ttataaacag gagaaaagtt ttgaattcca aaaacttgtt
     4261 tgagagattc tgcaactgct gtgtaatcag ctccattgag gtaagcgtgg gcacggtcgc
     4321 gatctgctgt taccttaact tgagtataga tagacaaaac gtccgaaata ttattacgaa
     4381 gtttattgat gaaacgcata cggtttttac ccttggttga caactctccg tagcgaatca
     4441 taatttctga atactgcatg aatgctccta tcttactttt ctagtttgat tgtaaatcaa
     4501 ttttaacttg gtcaaaaact gctcgacctg actcatatca ttttccaagt ctaggctaag
     4561 acgcacagct gacttggcct tatctttgtc cactcccatg gcaatcaagg taccagctgg
     4621 ttttcctgcc ttagatgaac aagctgaggt tgttgagata aaaatatcat agtcttcaaa
     4681 ggcgtgaacg atgacttcac ctcgaacacc tttgattcca aaagtcagaa tatgaggtgc
     4741 aaagtcttcc tcatctgaaa agacaaaaat atccggatag ttcagaagag cttggtgaat
     4801 cactgccttc atctgcccag tcttgctcct aaagatatct agcttttcca tagataaacg
     4861 aagagccttg gctgtcgctg caatccctgc cacattttca gttgtcgaac gataatctcg
     4921 ctcctggcca ccacctgtaa gaagaggtgt aatcttcttg ccagatttga tatagacaaa
     4981 gccaacacca cggacaccat gaaatttatg actcgagaag gtcgcgcaat ccacccgttc
     5041 tgtcagatac ttttcagtcg gaattttggc aagcgcctga accgcatcaa cgtggaagga
     5101 aatagtcggc ttgtctgcca agaattctga aatagcctca ataggttgga tagagccgat
     5161 ttcattgttc acagccatga tggaaacgag gattgtatca ggccgtatca aacctgctaa
     5221 cgcctcaaca tcgaccaagc ctttcttatc aactggagca aaatccactt caaatccttg
     5281 acttttcaac cagagggctg actctttgac tgctggatgt tcaatggctg aaacaatgat
     5341 gtgcttgcca aactgagctt tttcaaaggc cacacccttg ataacccagt tatccccttc
     5401 tgttccacca gaggtaaaga agatttcatc gcttttctta ccgactaaat ctgcaatctg
     5461 ttggcgagaa gcatctaaga ttcgtgttgc ttggtctccc aaacgatgga gactagatgg
     5521 gtttcctaaa atttttgaag cgacctgcat ataggtttca agggcttcag gataaggctt
     5581 ggtcgtcgct gaattatcaa agtaaatcat gtttctcacg ctttctaaaa tcactccctc
     5641 tattgtatca tgaaacggag cttgcgacaa gaaagggcaa tttctctttt tttaagattt
     5701 ttttaaagaa acagagtata ataagtcata atcaaaggag agaatgtatg tctaactatc
     5761 gtagaacttc aaaaccgaaa acagaacata tcaaaaaagg ctttactgtt tttcaaaaaa
     5821 ccattactac tatcggtagt atccttggtt tgattaccgc gggtatcacg atcatgaacg
     5881 ccttggataa taataaaaat actaaaaaag aacctacgac aagccagacg acaacctttg
     5941 tcaaagaaat tcaaaaagaa tcccctcagg aaaatactac tccaaataag gaaaataaca
     6001 cttctcaaga aaaaacacaa caagaagaaa cgccaaaatc tagcgtcaag gaagagaaaa
     6061 aagaagatca gaaaacagca actcaggact ctactacacc tgctacaagt aaacctgcca
     6121 ctgaaaatga aaaacagccc aatactccaa cttcagaaaa taaaagtaat caataaccag
     6181 aaaaatagct tccttccaac cttggaaaga agctattttt tattgctgca atacttttct
     6241 tggcttggta ccttcagctg gaccaatgac acctgccatc tcaagctctt ccatgagacg
     6301 ggtcgcacgg ttaaatccaa ctgacaaacg acgctgaatc atggatgcac tggctttctg
     6361 tgtttcgata accaaagact tagcttcttc aaaaagcgga tcaccaccag catctccatc
     6421 cgaaaattct ccttcatttt cagaaacctc acctggatca aaactctcat cgtagtctgc
     6481 atctgcctga gtcttgatga agttcacaat acgctcaaca tcgtcatccg agataaagga
     6541 gccttggaga cgaactggat gattttcatc aatcggttta aagagcatgt ctcctcgacc
     6601 aagaagtttt tctgctccat tttcatccaa aatcgtacgg gagtctgttc ctgatgaaac
     6661 cgcaaatgct acacgagatg gaacattggc cttaatcaaa ccagagatga catcaacaga
     6721 tgggcgctga gttgcaagaa tcatgtggat acctgcagca cgcgccttct gcccaagacg
     6781 gatgatagca tcttccactt ccttgctggc caccatcatg aggtcagcca actcatccac
     6841 aatcacgaca atgaatggta gcggaatttg cttgtactca gactgggaat tgaactcttc
     6901 taccttggca ttaaaacctg caatattccg aactcccacc ttggcaaaga gttcataacg
     6961 gttttccatt tcatccacaa ccttttgcag agccttgctg gctttacgtg gattggtcac
     7021 aactggaatc aagaggtggg gaatatcatt gtaaacagat aactcaacca tcttgggatc
     7081 gaccatcata aatttaactt gatctggtct cgccttcatg agaatgctag caataatgcc
     7141 gttaactgct actgacttcc ctgaacccgt tgaacctgca actagcaagt ggggcatttt
     7201 agaaaggtca aaagctcttg cggttccatt aacagccttc cctaaaggaa tttccaagaa
     7261 attttctgct ttcgtttgcg attgttccca tagttctcgg aaagatacag tggcaatatc
     7321 ggagttgggc acttcaattc cgattaggga tttcccagga attggtgctt caatccggac
     7381 atctttggca gccaaggcta gagcgaggtc atctgataga ttggaaatgc ggttgaccct
     7441 tacaccaaca gccggcttga cttcatactt ggtcactgat ggcccaattt cggcccgttc
     7501 aactgttacc ttaataccaa agctagcaaa ggttgcttct aagattttga tattttctct
     7561 gacaattttc ttctctttag actgatcttt tggtttatct ggtgcaaaga gttgtaagct
     7621 tggaagtttg tattcaaggg cttctttggc tgaaaaatcg acctgaacat cttcgtcatc
     7681 tgagtcatct tcctgttcag ggaatttaag ttcagtttga ggcaggatga tttctggttc
     7741 cacccacttt tcttctggaa taggtggaag attttgaaca gcttcctctg tcagaatttc
     7801 acccgtttcc atatcaacag gaggcaaatc gagtaaggct ttttcagtct cttcttgttc
     7861 taatctagcc tctttctcag ccttttggcg agctttttct tcttgtttga caaagcgttc
     7921 ctcttttcga cgctcgtgcc cttcccacca tttggcaaag cctctactga aaaattcagc
     7981 aatatcgtaa acagaccaag ggctgactag gagagaaccc actaaaatca agatagaacc
     8041 aataaagtaa gttccgatat ttgaaaagag aaaggctgtt ggaatataaa gagcgacccc
     8101 gatcaagccc cctccagcaa agctagtcgt tcgaaaacca gtcagatctg tcacaacctg
     8161 agccatggtc ccttttagaa cggacttgtc caaaccatat ttccaaacca agtaggcttc
     8221 aaaaatcaag agtaagccag caaatatggt gaaaaagcca gataagagtc cttcctgttt
     8281 tcgtatccac ttgaaaaaga agagatagat taataggccg aatatcgcca gataagctag
     8341 gctacccact agcaagcgaa ttaaattata aagggttata cctgcagccc ctaatttgaa
     8401 ggctgcgaaa atcaataaaa tcgcaattcc taacgaaatc aacattcgtt gaatcgcttc
     8461 ttttctttcc agttctgctt tagacggtct ccgtcttgtt gtacttgtat tcttgtttgc
     8521 cattcttcta ttataccata tttcacaggc atttcgggaa gagaaaaaga ctgcaccaaa
     8581 cggttcaatc ttttcatttt tctatttttt atgcttgtgg tttgcgtagg taaccataaa
     8641 ccacaccact tacgattgct cctaccaaga cagaaacgag gtaaaggaga gcatttgaag
     8701 taagggcgat aacgaagatt cctccgtgtg gcgccatgag tttgatacca gtaagaccaa
     8761 cgagtccacc tgctactgct gaaccaagga tgaagcttgg aatcgcacga gctgggtcag
     8821 cggcaccaaa tggaatcgct ccctcagtga taaatgacaa gcccatgatg atgtttgtca
     8881 aaccagagtt acgttcttcc ttagtaaatt tatctttgaa aagaagagtt gcgacaaaga
     8941 ttgcaagtgg tggcaccatt cctccagcca taactgctgc catggctaca gaaccacctg
     9001 aagaaacagt tgctgcaagc gtacctgtac caaagacata agctgcttta ttaactggtc
     9061 cacccatatc aacagccatc attccaccaa ggacgatacc aagaaggaca gctgaacctc
     9121 ctccaagacc gcctaggaag tcattcatag cagtgttgat tgcagccatt gggatattta
     9181 cagctagcat aacaaatcct gtcaagattg ttccaagaag tggcaataga aggattgatt
     9241 tagcaccttc gagtgaacga ggaactttaa cgtatttctt gatggcaaga accaaggcac
     9301 ctgcgataaa tccaccaaca agggcaccta ggaaaccaga tgagacacct gcaagagttg
     9361 aagttgcttc accacctgcg gcataaggaa ttttaccaaa ggcaaaacct tctttggcaa
     9421 tagcaccagc cacgaaacct gctaccaaac ctggtttttc agcaatagag taggcaacat
     9481 aacccgcaaa gactggaagc atcaaaccaa aggcagctcc accaattttc atgaacatag
     9541 aagctaactc atggtaagaa ccaagattgc caaggttttc atttggaaca cccaaagcac
     9601 cgtcaatcaa gaaggcaagg gcaatcatga taccaccacc gataacgaat ggtaacattt
     9661 gagatacacc actcatcaag tgtttgtaga aggcaccacc aaggctttgt ttttcgttag
     9721 aggctgttgc agcttttgca ccattagcgg cacggtagac ttcagtatct cctgaaagag
     9781 ccaagttaat tagctcttct gtcttacgga taccgtcagc aactggacga ttgatcaatg
     9841 gttttccatc aaatcgatcc atttcaacgg ccttgtctgc tgcaatgata atagctttag
     9901 ccttacggat atcttctgca gttagttgat ttccaacacc gctagcaccg ttggtttcga
     9961 ccttgatacc aacccccatt tcagcagcta ctttttgaag ggcttcttgg gccatgtaag
    10021 tgtgggcaat acctgttgta caagctgtaa cagctacgat aaagtcacca gagtcattag
    10081 caggtgcttg aacaagttcc tcagtttttt ctgaagcttg gtcaaaaagt tcgataactt
    10141 ggtctgcaga tgttgcttga cgaagtttgt ctgcaaaacc gtctttcatc aagtattgag
    10201 acaattctgc caaggctgcc aagtgagtat cattggcacc ttctggagct gcaatcatga
    10261 agaagaggtc agttgcttgt ccatccaagc tctcgtagtc aacaccctta tttgacttag
    10321 caaatagaac tgtcgcttct ttgacagcag cgtttttgct gtgaggcatt gcgattccat
    10381 cacccaaacc agtagaagtc aaagcttcac gcgccaaaat tccttcttta aatgtttcaa
    10441 aatctgttac ataaccgtgg tctgtcaaat ttttaatcat ctcgtcgatg acagctgttt
    10501 tttcagttgc ctgcaaatct agcaacatga catcttttct caataggtct tgaattttca
    10561 tcgtttttct acctcaactt ttccatatgt ttctttaata aattccgccg ttgccaagtc
    10621 atctgagaag gtagttgccg ttccgcaagc cactccccat ttgaaggctt ctactgcgtc
    10681 ttttgatttg acaaattcac ctgtgaatcc agcaaccata gaatcaccag ctccaactga
    10741 atttttgact gttcctttga ttggtttagc gaagtaagct ccctcagatg tgacaagaag
    10801 ggcaccatca ccagccatag agataataac attttgagca cccttagcca gtaactcacg
    10861 agcgtatttc tcaatttcat ctaaactttc gagtttaacc ccaaaaatcg ctccaagttc
    10921 atgattgttt ggttttacaa gaagaggctg gtagtccaaa ctatcaatta aggtctgtcc
    10981 ttcaaagtca cagaccactt gcgcaccagt ctggcgcgtc aaggaaatca aatccttata
    11041 gataacattg cctagatttt tagcacttga acctgcaaag acaactgtat cttctgctgt
    11101 cagactagat aaaatagctt tcaattcttc tagctgaacc gattcaacag ttggacccgt
    11161 tccgttgatt tctgtttctt ggtctgcttt gattttaaca ttgatacgag tatcttctgc
    11221 cacctggaca aaacgtgtct cgatttcttc ctctgctaaa gtatctgtga taaatttacc
    11281 agtaaagcca ccgataaatc ccgtcgctgt atttggtata ttcaaacgtt tcaagacacg
    11341 gctgacattg attcctttcc caccagcaaa cttatcatca ctgtccatac gattgacact
    11401 accaactttg acttggtcca aacgaacgat atagtcaatg gatggattga gtgtgactgt
    11461 ataaatcata cttctattac ctccgttttc tccttaataa cctgcaagag ctcatgccct
    11521 tgactagtga taacgatagc gcgtttgagt ggggctacct tggcaaagca agtttgtcca
    11581 atttttgacg aatccaccaa gacgtaggtc tgcttggcat tctccaaaat agctcttttc
    11641 acagctccct cctccatatc aggagtcgta taatagccat cgtcaacacc atttattccg
    11701 ataaaggcac ggtcaaagtg caattggtta atctggttaa gagcaacgcc cccgatacta
    11761 gcatctgtcg ccgtcttgac gtttcctcca accatgacag ttggaatctg cttttcaacc
    11821 aactgagcgg catggtgaat ggagttggtc acaactgtaa cattcttatt gaccaattca
    11881 tgaatcaaaa aagcagttgt tgttccagca tcgataaaga tgacatcttt ttctttaatg
    11941 agagaggctg ctttctgagc cagcaatttc ttttcttgaa ggtttttgac agatttttct
    12001 tgaatggttt cttcttcctg taaggagtag gggagttctg ctccaccatg cacacgacga
    12061 agcttgtttt ccgcttccaa ctcatccaag tctcttcgaa ccgttgattc tgacgtttct
    12121 agcaaactaa ctaatttttc tagagaaact acatgatgtt gatttaactc ctctaaaatt
    12181 agttgtttac gttctgtttt taacactgaa tcacctcctg ttatcgttta cactattcat
    12241 tctatcatac tttctttcga agtcaagcat tttttatcat tttctttcaa aatctatcat
    12301 tttttcttat ttctagaatt tttattgcaa gataagagcc tttatggtag actatttgag
    12361 taagtattgg aagattactc aagaggctta agaggccgtg ttggaaacgc ggtaggcgtg
    12421 taatagcgtg cgtgggttcg aatcccatgt cttccgtcga aaaaagatta tttctaggat
    12481 gcgacaaatg ttgtatcctt ttttctatcc tttttttaaa atagggtctg tttgtgactc
    12541 cctttttagc aaaagaatgc aaaggaatac caaagataaa aataaaaaac gttgatttaa
    12601 caacgtttta ccaaggaatg caaaagaatg caaaggaatg caaaggaatg caaaggaata
    12661 atggagccgg tgggagtcga acccacgtcc aaacacctgc caacatattt gtctacaacc
    12721 ataggttatg tattatttta acagcccctc gacacataac tcaagtctag aaactgcgag
    12781 tctatcaatc tcttatcaag ccgctagaca agacttaatc gtatctcgct aataataaga
    12841 cctgtcattg aacacgagca atccaaatcg ggtcacgcct gctggttttt aagcagctag
    12901 agcgtaagaa gtgttatttt ttgcagttat atttaactga gcgtttacgt cgccacacga
    12961 gtcgcaaaat atgcctcata atgcctgtcg aatccgtaac gaccccaaaa gacaataaaa
    13021 ccattatacc ttatctaact caaaaatgca aaaagaaatc acatacctaa aaaagatact
    13081 attcgagaaa tttagcaaac tggaagacta actacaactt tctcagtgca taactttacg
    13141 attgtaaata gagcttacga aatccaatta acacatacct aagatg
//
LOCUS       DAVHIU010000045        13143 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVHIU010000045 DAVHIU010000000
VERSION     DAVHIU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.047"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            92..274
                     /locus_tag="VVN06_001144"
     CDS             92..274
                     /locus_tag="VVN06_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5562698.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            219..1068
                     /locus_tag="VVN06_001145"
                     /pseudo
     CDS             219..1068
                     /locus_tag="VVN06_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1120..1482)
                     /locus_tag="VVN06_001146"
     CDS             complement(1120..1482)
                     /locus_tag="VVN06_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562699.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1773..3653)
                     /locus_tag="VVN06_001147"
     CDS             complement(1773..3653)
                     /locus_tag="VVN06_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU5562700.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLATVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3647..4516)
                     /locus_tag="VVN06_001148"
     CDS             complement(3647..4516)
                     /locus_tag="VVN06_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5562701.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4600..7245)
                     /locus_tag="VVN06_001149"
     CDS             complement(4600..7245)
                     /locus_tag="VVN06_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU5562702.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNRPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDDTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEYALECHQAQERFSAYRSAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQICPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(7336..8616)
                     /locus_tag="VVN06_001150"
     CDS             complement(7336..8616)
                     /locus_tag="VVN06_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5562703.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPFQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8787..10871
                     /locus_tag="VVN06_001151"
     CDS             8787..10871
                     /locus_tag="VVN06_001151"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU5562704.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10911..12590)
                     /locus_tag="VVN06_001152"
     CDS             complement(10911..12590)
                     /locus_tag="VVN06_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5562705.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDDLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3659 a         3116 c         2676 g         3692 t
ORIGIN      
        1 ctgagtaaaa actctcgaaa ttcaattcgc tatattatca ctcaacttga acaaatacca
       61 gaaccacaaa aaacagatgt aaaggaaaaa tatggactta aaatttgaag gggttgattt
      121 agaatacaaa aaagctaaaa acaatcttcc agagtctttc tgggaaacct actcagcatt
      181 tgcaaataca aatggcggga aaataattct cgggatagat gaaaaaaaca ttgacaccta
      241 ccaaagagtc aatagactcc ctgcgaaatt atgaagcaag taaacaatta actgatacac
      301 gatttaagcg tcttgttggt gttcagcaca cgacttttaa agagatgtta gctgtattaa
      361 aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta agcctagaag
      421 accttcttat gggcactctt caatatgtgt gagaataccg cacttatgaa gaaattgcgg
      481 ctgattttgg tattcacgaa agcaacttac tccgtcggag ccaatgggtt gaagtaactc
      541 ttgttcaaag tggtgttacg atttcaagaa ctcctctcag ctctgaggac acggtaatga
      601 ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaag aattagcgaa tgattctggt
      661 aaaaagaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
      721 tctttggata tcactgtgaa ctattgtcat gatatgaagt tgttcaaaat gagttgcaga
      781 aatatcggac aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata
      841 tgtcctcaag cacaaacttc acgtaaatcc agcaaactca agccactaac agctgaagat
      901 aaagcctata accatgcgct atccaaggag agaagcaagg ttgagaacat ctttgccaaa
      961 gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
     1021 cgaatgaatt tgattgctgg tattatcaat catgaactag gattctagtt ttgcaggaag
     1081 tctattgaaa tactaagcct acacagactg atttcaattt taccattctt catagtcaaa
     1141 accatcataa tctggatatt gttctggcga cgttccaacc gaacgtacga gataagcttc
     1201 ttcgtcctct gtctcgattt ctctcagcac ttggcattca aaacgccatt catcaccgaa
     1261 gtcgaaaata aacttaaatt tttgtttgac acttaggcta tccagataga cattttctgt
     1321 gtaacgttct tcaacatcgt cactaacaaa gctaagaaag taagaatctg catgactcca
     1381 ctcaacatta tccatgaaaa atgcgtgagc atgatcattt gcaaaatcaa aactccataa
     1441 aatcacatct gctagttccg ctaacgtttt tgtttttgga atctatatat gtcggtagca
     1501 acctttttta aatttgacgg aaatgaccca agcttctaca gatgctggct tttcagcttc
     1561 tatttcttgt tttactaatt ttaactgtag catttgaaac cagataattc ttctgtttca
     1621 cgcgaaacac cctgcgccaa gcaaggtgtt cttgttttta tactcaatga aaatcaaaga
     1681 gcaaactagg aagctagctg caggctgctc aaaacactgt tttgaggttg tagatggaag
     1741 ctgacgtggt ttgaagagat tttcgaagag tattaagtcg tataaatcgt cgaagctgtc
     1801 gcaatggtat gccactggtt agctgtagtt gctgcgaaat ccttgtctgc gtagaagtca
     1861 gtaaatggta ggatttcaac ttccagctcg tcgatgcggt caagctggcc agccagataa
     1921 acctcgatac ggctttctgc tcgtttgatg cgttgcaaga gtccgcccat acggatgtca
     1981 actgtatcca aaccaaagac cttgttttct ttcagccatt ggtgactaaa gagggcatgg
     2041 aagtcttcaa tttggcttct aagttctggt aattcttgtc tggcgatttg ttgtaaactt
     2101 tctttatcat ccgcttggta ggcctgacga atgcgtcgtc ccacatctac tttgctactt
     2161 aaaatagcat tcaactgggc ctgagtttca aagagataag catagtttcc agctttttct
     2221 ttaatgtcag caatagttcc cgccgcctga gcgaagtgtg gcttgtcctg ttcaggtgtc
     2281 atgtgtcggt caagtatcgg acagagaaca tcctgataaa agacatatcg gttgggattg
     2341 atgccactga gattacctgg taggtctggt aagaggttgg caaggtcaat ctgcataaaa
     2401 tcttcaaccg ttagaccagt attggtcttg aagtgcgcag acaaactatc taggtcattg
     2461 cgatagctga gttctgccca gatttgcaag cttggtagga tagagaactg ggcagtttca
     2521 ccaccattgt ctccccaacc cgttacgatg acttctttaa tctgattggc acggcaggct
     2581 ttattagcct cgatagccac tagacggcta aaatggttgt gaggtgtaaa gccaatccac
     2641 ttccaagctc cccctgcaaa tgcaaggtca tggctaatct tgtgatgatt gcggaaatta
     2701 cggttgtatt tttcctcgct atcctgataa taatcccagt aaaccagagt cacacggtct
     2761 ttgagacggt ctaggtagac acgagtttcc tctggaattt ccacatcacg atcgtactgg
     2821 ccatccgctg acatgagttt gaagaacata tcactccaca tctggcagtg gaaaccatat
     2881 ttgtcagcaa tatccagcac gcgctccaag tgttggcaca tgaggagact acgatccaca
     2941 acaccgttca gaatcaggta gcgtcccaaa ccaaccaagt gggcttcgtc catcccgata
     3001 ttgaccttgc gagtcttcag tttagacaac gtggcaaaca tgccatcaat caggtcataa
     3061 accttttctt cgccaatgag aagaatgtcc tctacatcac ggagctcctg cacttccttg
     3121 acaccccatt tgacaaaggc cgacaagtgg gccaaggtct ggatgcatgg tacaaaggtc
     3181 atgtcaaact gttgagcata ggcttcgatt tcctgcaact cctctgctga ataagctcca
     3241 cggaaatagc caaagtaagg ctgtccttca atctggtaag tgtcttccat gtaaagctca
     3301 aaggttgagt agcccatgag agccaagacc tcaatcatct gcttggcaga agcgacattc
     3361 agcaccgcat ttcgagaaca gtcaaccatg taagccaaat cttcgtaagc tgcttgttcc
     3421 tcaatctcta ctttatcagc ttctgctaga actgttgcca acaaggacaa ggcacgataa
     3481 agttggtgag gtttgcggta ggtcaattga tagtgacctt cctcaccctc gatagagata
     3541 gaggcttggt cagactgagt gacagccact tccacatctg gtagagaaat atgacctttt
     3601 aaaacctcaa tagcttgcgt ttgtttggga ctaagtcctg taaatcttac cattgctttt
     3661 cctcctgtag ccagttgaca agagcaccgt agagattggc atctgcgtga taggtgcagg
     3721 cctggataac tggtgcgacc gtgtattctt cgtaggcatc gacaaagtct tcaacagcct
     3781 tcttgacacc ttggataaaa tctggatttt gactgataga gccacccaga ctgatgacat
     3841 ctggatcgat gagatactgg atattgagca agccttgcgc cagattgcgg ttcattcgct
     3901 caatggcttc ttgacaaaga gcattaccag ctgcggcctc ttggtaaatc ttgcgaccgt
     3961 cccaatcagt atgaccagat ttttcaatca cgtatcgtac catattccca gttgatgcta
     4021 gttgcgacca gttattaagt ttttcagcag gggcaagggt tgtcatgtag ccaaattctc
     4081 cacccagacc gtggcgacct cgatgaagtc taccattgat aatcatggct ccgccaatcc
     4141 ctgtcccaat cacgacacag gctgcatttt caagctctgg atgagctagt agttcactga
     4201 gtccaacgca gttggcatca ttttctaaat ggacaggtag ctgataagag ctaagcgcct
     4261 cataccaaga aaagccatgg atgtagggca ccgcactgaa gccatcaatc acacctgtct
     4321 cttgattgac cgcacccgga acgctcatag caatcccact gtaatcctgt tctgacaagc
     4381 gttgatctag ccacgctagt aaatcctcca agttttcagg cgttgaaata cttgtcttat
     4441 ccagtatttt cccatcagga gtcagactgg caaacttaat cccagtccct ccgatatcaa
     4501 tcgttgcaat ggtcattgtt tttaccataa aaaggggcga atcagcagtt agttcttact
     4561 ttttcgcccc tcctttcttt tgatgtttac tttttgaaat taaatttctt cttttttgat
     4621 gaattctgta cgaatctctt gtggatccaa tagtcctgaa tgaactggat atggtcgctc
     4681 aagtaagtca agaatggttt gttggtgttc tgatatgcgc acattttctt gactcatatt
     4741 gtagtaacgg agtacatatc cttcttcatt ttccgccact ttaaaggctg ttggacagat
     4801 ttgcggtatg ctgagaacag aatgactcaa gaggctacca gtcgcagcca cgcttccttc
     4861 ctgtctagca agctgaaggc tggtaaacgg tgtctgcaag gctttggcac tacgataggc
     4921 tgagaagcgt tcttgggctt ggtggcactc aagcgcatac tcgacttcaa actcacgcaa
     4981 gcattgggct tctggtgttg ggaagtagcc ccagtcacct agctcacctg atgcacgcaa
     5041 aatagtcact gcaatggtgt cgtctccaag gatttcgtat tcattcaatc ccttgttgga
     5101 tacagtcaca cctttttcat cgtcatacag actaacaaag gcttgttggt gttgaggatt
     5161 ttcaggattt tcccatgaag cagctggtct gtttggacgt gtcaccacct cataaatgct
     5221 ttcagaatca ttgcttggac gcgtgttatg agtcttgacc aagagacgga tacggtgatc
     5281 cttggcagtg ttagtaaagc gagtcttgaa gcggatttgt ggattgtcaa cgaagacagt
     5341 caactcagtt tccagaggaa tgtttgtcaa ttcttctgac cgtccagcct cacgcttcat
     5401 aaactcgatg ataccttgtt gctcttcttc tagcttttca tccgcgctga caggcacggt
     5461 caattcatgt ttgagcaaga ttttagcata gcaagctgtg ttttccaaga cctcgtggcc
     5521 cttaagctct gcaaagattg gctctgttcc ttttggttgg aaatagatat actcgtttcc
     5581 gatgtcccca cggtcttcaa agcggataaa gtcttcatag gcttcgtgag ttgtcttgtc
     5641 atagactgtg atgttgtcat ccacactcac cgttacgaat ggtgtatcaa tcactccgtt
     5701 ttggtaaata ccctcacggt gttcttgttt tccttccagc aattggaagg ttgtccaaga
     5761 aagcggcgct aggtgaactg gaatggtcac gcgcacttga cgagcaatac gagcttggcg
     5821 gaacttgtct tttggtaaat catactcaaa attagctccg aggtcttcga ttgtagcctc
     5881 tacaggatga ccatccaagt cctccacacg gtaacttggc aagataagag cagccatctt
     5941 tttgtagcct tctgttgggt gcaattcctt gaaatcacaa gtcgccacat caatcactgt
     6001 gctgacagta tcgaccttat catgcaagcc tgtgttaatg acagtaaaga gatagtcact
     6061 ttgagcctta tccgtagcaa ttttaccctt ccactcgttg agcaagttac ttttaacaaa
     6121 gtttcctact tggttgacct tggcaaaacg cgtttccatc tcgcggtgaa cttcgtccac
     6181 gctacagcca cagatactat catgtggcgc gttctgcaaa agtgttttcc aagcataggt
     6241 caactggtcc ttgtggttgt gtccaccagt gataatagtc aagggttcta ccacttgctc
     6301 taggaggttg ctattttctt ggaaggcttg ttttaggtaa atgcgggatg aagaagtgtt
     6361 ggcaagtgtg taccagccat ctgtttcctg actggtcaac tcacctgtaa cagttgataa
     6421 gtgttcagga agcgcacctt ctacagcttg aacatattca tcaaaagaac tatgaacaaa
     6481 gattacatct gggaagagtt catttgccac acgaatggct tcgctcagat ttttctgtac
     6541 aggctggtgg tcacagccgt tcatcatcaa ccattggttg gtcgaagcgt aggcacgcac
     6601 atctgacaat ttttgtttcc agaaggtcaa ggcctcatct ttgtcaactg gaatttcatt
     6661 cccgttactg taccagttgg caaagagaat acctaaaaca cgactaccat ccacaccctg
     6721 ccagtacatt tctgaaaact gggatgtaaa ctgctcatct tcaaggactt ggttgtcaaa
     6781 tccaatcggc ttcacaccac gaccaaaggc cgccacgtga atgcctgatt tttgaagaat
     6841 ttgaggcgct tgtcccatat ttccaaaggt atctggaaag tagccaatct gggttgattt
     6901 accccatttg gcagcttctt gttgaccaat caaggtattg cggacattgg cttcactgga
     6961 gatcaagtag tcatcctgca agatgtaaaa gggaccaatt ttaagtttgc cctcgtcaat
     7021 gtagcgttgg accttgtcac gattttcagg gcgaatttgt aagtagtcgt caaggacaat
     7081 agtttgtcca tccaagtgga aactcttgaa ctcagggtca ttttcaaaga gatcaaagag
     7141 attgtcaaac aattccacca actgcatacg atggctttca aaaggcaagt accactcacg
     7201 gtcccagtga ctatgtgaga taatatgtac aacaacattt tccatgaagt aaaacctcat
     7261 tctaaattta aattttaacg ttttaaatgt aaacttgtga ttctgataag aatcggttga
     7321 gctaaagcga gccccttagc gaatatccaa gtaatccaag accaactcac agaacatcat
     7381 gttagcccag gagaaccatt cacgagagta gagggtcgga tcatctacgt ggaagctttc
     7441 gtgcatgaca cctgtaccac catcgcaggc aaccagctga tccagcaaga attttttctc
     7501 tgccttatct cttgttgtca atccttggat agaaagggcg attggccaga tatagcgata
     7561 gaaggtatga gaacttccga gaccgctagc gtattctcct tggtagaagt atggattttc
     7621 agagctcaaa atggtacgac gagtagcttg atacacttca tcatcgaccg aacagtagcc
     7681 cagatagggc gcagctagta gacttggtac atttggatca tccatgatgc tggcatttcc
     7741 taggccatcc acttcaaaag cgtaaatctt ttcgcccttg ctgttggtgg tgtaagcgta
     7801 gttttcgatt ccttcttgga tttcatcctg aagacgcttg gtatcagcaa taacactctg
     7861 gctatcagct aggtttaatg ctgcgaagat ttcttgcaca taacccaaga ctactacagc
     7921 aaacatattt gacggaatca agtaactata ctggcaacag tcatcactcg gacgaaaggc
     7981 tgaccaagtc atacctgtca ctgcaaagtc aggtccaaag ccatcattta ccaaggtgtc
     8041 ttccttacgg tccgtatctc ggacaaaacg ataaggagag ttcttgtggt cttgttcaac
     8101 tgtccataga tggagaattt ccttagtcgc tgcgacaaaa gtctcatcaa actgaccagt
     8161 ctcgccagtc tctttccaga gaagataagc caactgaaaa ggatagcaaa gcgagtccac
     8221 ctcatacttg cgttcccaaa tccagccatt aaggtcggtg tggtcggtct cgtggtgccc
     8281 cttccagttt tcctctatgt tgaaggaatt ggcataagga tccttgagta ccaaggtcat
     8341 ctgacgtttg accaaacctg caatggtctg acgcaggagg gaatctcttt tagccacatg
     8401 caggtagggt ctgagttggg ctgtcgaatc ccgaagccac atagcaggaa tatccccagt
     8461 caagacaaaa gttgaaccat cttctaagat ttcaaccgta ttgtccaagg tatcggtgta
     8521 gcaacgctca aagacatcaa cccactctgg atggtccttg gcccgctctg ctacttcatc
     8581 cagccactct ctgacgattt ctttcgaata aatcatcgtg caattccctc tttcaaacaa
     8641 gtttcatgtt cagtatatag cttttgagac agaaaataca tacacaaatg atagtcattt
     8701 ttctacaaaa tagttatttt tgaaactcct tttctaaagg ccctcttttt ctgatataat
     8761 gtagaaaaac agctaaagga aagactatga aaccactact tgaaaccatc gatacccgct
     8821 ttggaactac caacaagcat gccttttctc ggggaaatac cctgccatac acaggcgtgc
     8881 cttttgggat gaattacttt gtgccccaga ccagtgacca ggatggatct tggttttttg
     8941 atccgcatct gcctatcttt caggggattc gattaactca ccagcccagt ccttggattg
     9001 gggactactc ttggctcctt ctgacacctg tcaccagtca actgggggga gacagcctct
     9061 ttcaccgcca gtcttcttat gatatagata aagcttgctt ccagcctcac tatttgaagc
     9121 tcttttctct gcgttatcag attgaaaccc agctcacacc gacttgctat ggtgcttcta
     9181 ttcgtttgaa tcaaaagcaa ggcaaagccc tctccctcta tcttcacgca gcagatgaac
     9241 tgacagtaga gcaagtagat aagcggactc ttgccctgcg acaagaaggt aaaactgaaa
     9301 ctaacaaaaa ttcgctaacg atgttcactg ccctgcaaat gaacacggat attcttgcca
     9361 tcagccaaga agctggagac tggcgaattg acttagcaag tagtcaaacc gagatgcaac
     9421 tagccacttc tttcatctct ccttctcaag cactgcttaa tctacctcaa gaagattttg
     9481 atagctgtaa atcaagtgcc caagcggatt gggaaaatct cctccatcgt tttgacatta
     9541 tagagatagg agaggctgac cgaaccttct ttgaccactg cctctacaga ctcttcctct
     9601 tcccacagac tttttatgag attaatgaat cagggcaagc catccacatg gatctggcta
     9661 ctggtactgt caagcctggt gtcctcttta gcaacaatgg tttctgggat accttccgca
     9721 ccaccttccc cctctttgcc cttatcatac cggagcacta tcaacgcttt ttagaaggtt
     9781 tcctcaatag ctaccgcgat actggtttcc ttccaaaatg gctggctcca gatgaacggg
     9841 gtatgatgcc aggtacactt ttagacggta ttatcgcaga tagcgcctgc aaggacatgg
     9901 cccctgacct agaaggagaa ctcttccaag ccatgctcga aacagccagc aaagccgacc
     9961 ctctcggaat caatggccgc cacggactag cccaatacca agaactaggt tacctctcta
    10021 ccgaccacca cgaaagtgtt agccatactc tagactatgc ctatagtgac ttttgtatcg
    10081 ccagctgtgc caaaaaacta gagaacatag aaatcgctga aacctacaag gctgcttcac
    10141 aaaattaccg ccagctattt gacgctgaga caggttacat gcgagcacga gacaatcaag
    10201 gaaactttca ccctgacttc tctccttata gttgggggcg agactacgct gaatgctctg
    10261 ccattcaagc tactttaggc gttctccacg acatccctgg cttaatccaa ctgatgggtg
    10321 gaaaagaaac ctttagcaac tatcttttga aagcctgtca agatgctccc ctctttgaaa
    10381 caacaggcta tggttacgaa attcacgaaa tgagcgagat ggctactgct ccttttggac
    10441 aaatagccat ttccaaccag cctagtttcc acattcctta tctcttccgc tacagcgatt
    10501 accctgacta cactgccctt cttatcaaga cgctccgtca gaaagctttt cacccaagtt
    10561 gggaagccta tcctggagat gaagacaatg gtagtctctc tgcttggtac atctggtcag
    10621 ctctcggatt ttatccaacc tgtccaggaa aaccaagcta tgacctcgga atccctctct
    10681 ttgaccatct acgagtctac ctcgctaaag aagataaatg gctggatatc catactaaac
    10741 aaaaccacaa ccattttaac tttgtcaaag aatgccgact ggacaaaaca ctcgtatcaa
    10801 ctattcaaca ccaagacctc ttaaaagctg aacaactaac tttcaccctc agctggttac
    10861 caagtcacta ataaccaaaa gaacctttgc atcgcgcaaa ggttcttctt ttatgaaact
    10921 tggacttgaa gttgatccac aagctgacca tccaccgtta ggttcagata gaaatccaag
    10981 gttttatctc ctgcgaaata cagggttggt agatgcaaat cttttttgct ttcccctgct
    11041 tggaattgaa ggacttgaat ctcatcctga taggcgacac catggacacc tgtccccggt
    11101 tgaatcgaaa tcttagcctc taaagaggag ctactttcac tacgttcaat cctaaaatga
    11161 agagtctctc cctttgccac agctaggctt ttttctgcaa atgctagccc ctgaacagct
    11221 actgtttgtg ataacctcgg agttttatag agtgaaatct tggtcaacaa aggcaaagcc
    11281 tgtgattctg taatgactac acgtatcttc tgtgcttcaa caactgctcc tcgtaagaga
    11341 cgtttgtaac caacagtatg acccgaacca aactcctgcc agacaccatc cacctctact
    11401 tgcacatgaa aagcagcgat tcgttgccct agcttcaaat cttctcttaa ctcaattaca
    11461 tcaaaagttt taggagaacc taagtcgagt tctaactgga tgggcaagtc tgcatcgttt
    11521 gcccaagagc tggtctcaag gtcgtctgtc aaatggcgac aagcaaagtc tgcggaaaga
    11581 gctggaccag atacctcagc tcccagagcc aaatcttctt tatagagctc attgcgatag
    11641 gtcgcaaatt cataaagtcg ttcaatatcc tttgcatcaa agagcccagc ttgattcggc
    11701 ggaatattaa gcaagagtgg agttcctcgc cctactgagt gaaagtagat ttcgaccaac
    11761 tcctcgagag acttaggatc ctgatcctca tggtagaacc agcctggacg gatggaaaca
    11821 tctgcctctc cgattgaaaa aatcgtgccc gagggatccc cgtgctgaag atagttcagc
    11881 tctgcttctg ttcctagttt atcaggattc accttttgcc acagtggatc acctgcatac
    11941 cctcgttcat tgccaatcca gcggatactg gtgccttctg ttgaaaaaat caagcaagcg
    12001 ccctgcaggt cacgaatggt ttcaaaccat ttttcaaatt cataattaac cttttgcgcg
    12061 ccctctcctc tggcaccatc catccaaacc tcagcaaatt tccctccatt tccataggca
    12121 ggatttgata agatttcctt cagctgagcc agataatagg cattgtagtc cgcttctcgg
    12181 tccacatgat agaggggact atgggcatcc cacggtgaca ggtagacccc catatccata
    12241 tcaaactctg tggcagcttg ggatacttca aggagcaagt cgccctttcc tctcctccaa
    12301 ggactgacct taaccgaata atctgtgtga gctgtcggat aaaggacaaa gccatcgtgg
    12361 tgcttgacca ccaaaatcaa ctttttgaag cccgtttcct tgagcacacg aacccactca
    12421 cgcgcatcca actgactcgg gttaaagcgc tcaggatcct cctgtccagt cccccattct
    12481 tggtcataaa aggtattagg accgaagtgg ataaaagctg ctagttcatc tcccagataa
    12541 gctagctgag tctgacttgg taacggtcca tgcggtttga ttttcttcat cgttatatat
    12601 ctccttcttt agcaacttct cttgttcaca aggttatcca caggttgtgg ataataaacc
    12661 atgatctaac agccagctct ttccaactcc aactggtagg ctcaggattt tgctcctcaa
    12721 tattccctct tcaaacagct ctgtcatatg gttctgctaa cacctctcag aatgctactc
    12781 tccataatta ttctcaggct tctttcaggt cattgtgtga agaccattca ctctccagac
    12841 ttttccacag attgtgaata agtgattatt ctaaactatc acctcgtcga ctataacaaa
    12901 aaataaaaag agggtcaata agataattat ctcttcctaa atactcagaa aagattctat
    12961 aatttgtaca attgaacaag aaatgcttct ccaaaatcta tcttcaaaag aatgttgcat
    13021 ctttttatcc tttcacacaa acatttctct ttatacagat ccaagtgata accagatgat
    13081 aattggacac tttttggtta tttgtgtatt ttttgaaaaa ttttttaaaa aaattttata
    13141 tcc
//
LOCUS       DAVHIU010000046        12909 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVHIU010000046 DAVHIU010000000
VERSION     DAVHIU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.048"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..307
                     /locus_tag="VVN06_001153"
     CDS             <1..307
                     /locus_tag="VVN06_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562706.1"
                     /translation="LPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLYSLFSLAWTVF
                     SVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRELGKSKGVRH"
     gene            492..1283
                     /locus_tag="VVN06_001154"
     CDS             492..1283
                     /locus_tag="VVN06_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562707.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIVTYFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFFKIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1276..1917
                     /locus_tag="VVN06_001155"
     CDS             1276..1917
                     /locus_tag="VVN06_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562708.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLFAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            1921..2571
                     /locus_tag="VVN06_001156"
     CDS             1921..2571
                     /locus_tag="VVN06_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5562709.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            2582..2869
                     /locus_tag="VVN06_001157"
     CDS             2582..2869
                     /locus_tag="VVN06_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562710.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            3015..5528
                     /gene="secA"
                     /locus_tag="VVN06_001158"
     CDS             3015..5528
                     /gene="secA"
                     /locus_tag="VVN06_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5562711.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRK"
     gene            5609..6640
                     /locus_tag="VVN06_001159"
     CDS             5609..6640
                     /locus_tag="VVN06_001159"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5562712.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6642..7673
                     /locus_tag="VVN06_001160"
     CDS             6642..7673
                     /locus_tag="VVN06_001160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5562713.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7712..8080
                     /locus_tag="VVN06_001161"
     CDS             7712..8080
                     /locus_tag="VVN06_001161"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5562714.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8070..9173
                     /locus_tag="VVN06_001162"
     CDS             8070..9173
                     /locus_tag="VVN06_001162"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5562715.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9192..11207
                     /gene="recG"
                     /locus_tag="VVN06_001163"
     CDS             9192..11207
                     /gene="recG"
                     /locus_tag="VVN06_001163"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5562716.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKTEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11523..12504
                     /locus_tag="VVN06_001164"
                     /pseudo
     CDS             11523..12504
                     /locus_tag="VVN06_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
BASE COUNT         3719 a         2425 c         2917 g         3848 t
ORIGIN      
        1 attaccctgt ttttactttt tcagctggat tgacaaggat aatagagaaa gtaaattttt
       61 cccaattttt tactattttt actggattta catcaccctc tattctcttt ttagccttgc
      121 ttggacagtt ttttcagttc tgtttttcaa tatcgtcttg agaaatttga cagatatcaa
      181 gttatggggt atttggctat tattactact tatcgctttt gctagtgact ggttagacta
      241 tatctgtttc aaaaaaatgc ttgacttgag acgggaacta gggaagtcta aaggcgtaag
      301 gcattgattt atactcttcg aaaatctctt caaaccacgt caacgtcgcc ttggattata
      361 tatgtaactg acttcgtcag tcttatctac aacctcaaag cagtgctttg agcaacttgc
      421 ggctagtttc ctagtttgct ctttgatttt cattgagtat tatattactt tctatttgta
      481 ggaggttgct tatgaagatt cctctcttaa ctttggcaag gcataaattt gtttatgtct
      541 tgcttacttt gctttttctt gctttggttt atcgtgatgt tttgatgact tatttctttt
      601 ttgatattca tgcgcccgat ctagctaaat tcgatggaca agcaattaaa aatgacttat
      661 taaaatcagc attagatttt cgtattctcc agttcaatct aggtttttat caatcattta
      721 ttattccaat catcattgtt ttgctaggtt ttcaatatat tgagctgaaa aataaagttt
      781 tacgattgag tattggaaga gaagtgagtt atcaagggtt aaaaagaaag ttgactttgc
      841 aagttgcaag tatcccttgt ttgatatatt tagtgactgt gctgataatt gcaattgtaa
      901 cctatttctt tgggactttt tctcctcttg gatggaattc tctattttct gatgggagta
      961 gtttacaaag gctcctagat ggagagataa caagctattt gttctttact tgtgtcctac
     1021 taatcggtat tttcatcaat gcagtctatt tttttaaaat agttgattat ttaggaaatg
     1081 tgactcgttc ggcaatcacc tatttgatgt ttctttggct tggttctatg ctgctctata
     1141 gtgccttgcc ttactatatg gttcctatga cgagtttgat gcaagctagc tatggggatg
     1201 taagtttgat gaaactcttt actccttata tcctttatat tgtcccttac atggtgcttg
     1261 aaaaatatga agataatgtt taagaatttt aacaatattt tgctaaagag aaagattgtt
     1321 ttactacttc gtatagttct gatgatgatt ttgataaacc atctattgtc aacagcggtt
     1381 caaaagcagg atgctgttat ctttttcaag agagaattga tttcaatttt ttcctataat
     1441 gactattctg aagtgaattt agaaatcccc aaactcttgt taaacctttc gcttttcatg
     1501 gtaggatggc tctctgtcat tttacttgaa agtgatttgg cagaccatta ccatcacttg
     1561 attcgctatc aatcaagctc ctttttcgat tatacaagga aacgattggt tgtcatttct
     1621 aaatttttta ctcaagattt acttgtctgg ttccttggtt tacttccgct aggaattcat
     1681 ttcaaaacag tcgcactttt ctttttattt gctcagttaa tgatgttgta cttactactg
     1741 tcttatctga tagcactgat tagtgcgggc gctggttttt ccttttttct ctatttttta
     1801 gcatttgtgg gacaagaatg gatgatggat catattgtaa cagtgtattt actactctta
     1861 agcttattag ttatgttgat tgttagtcgc ttggaagaga aatttaagaa aggataaacg
     1921 atgaaacttg aaattataaa tggacagaaa atttatggga aaagacctat tttaaatcag
     1981 ttgaatttgg tgtttcaatc aggaaaaatt tatggactta aaggtgataa tggatctggc
     2041 aagacggttc ttttaaaggt acttgctggt tatattaagc ttgacaaagg aaaagttctt
     2101 caagatggta aagtttacgg gataaaaaat cattatattc aggatgcagg aattttaatt
     2161 gaaaaagtcg agtttttatc tcatttatcc ctgagagaaa atttggaact gttaaggtat
     2221 ttttcatcta aagttacgga aaaaagaatt gcctattgga ttcaatacta tgatttacag
     2281 gaatttgaag acgttgaata ccggcattta tccttgggaa caaagcaaaa aatggccttg
     2341 attcaagcct ttatttcctc tccttctata ctctttctcg atgaacctat gaatgctttg
     2401 gatgagaaga gtgtgaggtt aaccaaacag gtcattttat cttacctgaa aaaagaaaat
     2461 ggtctggtta tcctgacgtc gcacatatca gaagatattt cagacctttg tacagatgta
     2521 ttagttgtcg aaaatggaca tatacaatat gtaaaggata tacaatccta ggagatggct
     2581 tatggcacat ctaaaatcat ttattacacg ctattccaag gtttatattg gtttagttct
     2641 gctgatctgg ctgtctttct tctttatccc ttgggataga gcgattctgg gggtaaggat
     2701 tgacatcttc atcatacaga aagtcttgct agtttttgga attctgtcca ttttgatggc
     2761 cttgctgtcc aagaaagtca gtctctttgt ttttggactg atttgctgtc tttctctttg
     2821 gattaactta tttatcacat ttgccatttt gccaattttt ggcaattaaa cagtcataaa
     2881 agtcggagag gttagcttga aaactaacct ctttttcctt ttcaaaatgg ggattcttcc
     2941 ttgaaaataa tcagtaattg tgctaaaatt aaaggaacat tctaaaatat tcggaattta
     3001 aagtaaggaa aaacatggct aatattttaa aaacaattat cgaaaatgat aaaggagaaa
     3061 tccgtcgtct ggaaaagatg gctgacaagg ttttcaaata cgaagaccaa atggctgctt
     3121 tgactgacga ccaactaaaa gcaaaaacag ttgaatttaa ggaacgttat caaaatggag
     3181 aatcactgga ttcattgctt tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg
     3241 tcctaggtct cttcccttat aaggttcagg tcatgggggg gattgttctt caccatggtg
     3301 acgtgccaga gatgcgtaca ggggaaggga aaaccttgac tgcgaccatg ccggtatacc
     3361 tcaatgccct ttcaggtaaa ggggttcacg tagttacggt taatgaatac ctgtcagaac
     3421 gtgacgcgac tgagatgggt gaattgtact cttggcttgg tttgtcagta gggattaact
     3481 tggctaccaa atctccaatg gagaaaaaag aagcctatga gtgtgatatt acttactcaa
     3541 ctaactcaga aatcggattt gactaccttc gtgacaatat ggtcgttcgc gctgaaaaca
     3601 tggtacaacg tccgcttaac tatgccttgg tcgatgaggt tgactctatc ttgattgacg
     3661 aggctcgtac acctttgatt gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc
     3721 acatggcaga ccactatgta aaatctttga acaaagatga ctacatcatc gatgtgcagt
     3781 ctaagactat tggtttgtct gattcaggga ttgacagggc tgaaagctac ttcaaacttg
     3841 aaaacctcta tgacatcgaa aacgtggctt tgacccactt tatcgataac gcccttcgtg
     3901 ccaactacat catgcttctc gatattgact atgtggtgag cgaagagcaa gaaatcttga
     3961 ttgtcgacca atttacaggt cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc
     4021 aagctattga agccaaagaa ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa
     4081 tcacgtacca aaaccttttc cgtatgtaca aaaaattgtc tggtatgacg ggtacaggta
     4141 agactgagga agaagaattt cgtgaaatct acaacattcg tgttattcca atcccaacaa
     4201 accgtcctgt tcaacgtatt gaccactcag accttcttta tgcaagtatc gaatctaagt
     4261 ttaaagcggt tgtcgaagac gttaaggctc gttaccaaaa gggtcaacct gtcttggttg
     4321 gtacagtagc ggttgaaact agtgactaca tttctaagaa attggtcgca gctggtgttc
     4381 ctcacgaagt cttgaatgcc aaaaaccact atagagaagc ccaaatcatc atgaatgctg
     4441 gtcaacgtgg tgccgttacc atcgcaacca acatggcggg tcgtggtacc gacatcaagc
     4501 ttggtgaagg tgttcgtgaa cttggaggac tttgtgttat tggtacagaa cgtcatgaaa
     4561 gtcgtcgtat cgataaccag cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt
     4621 cacaattcta cctatctctt gaagatgatt tgatgaaacg ttttggttct gaacgcttga
     4681 agggaatctt tgaacgcttg aacatgtctg aagaggcgat tgagtctcgc atgttgacgc
     4741 gtcaggttga agcagctcag aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag
     4801 tccttcaata cgatgatgtc atgcgtgaac aacgtgagat tatctatgct caacgttacg
     4861 atgtcatcac tgcagatcgt gacttggcac ctgaaattca gtctatgatt aagcgcacga
     4921 ttgaacgtgt cgttgatggt catgcgcgtg ccaaacaaga tgaaaaacta gaggcaattt
     4981 tgaactttgc taagtacaac ttgcttcctg aagattctat tacgatggaa gacttgtcag
     5041 gcttgtctga taaggccatc aaggaagagc ttttccaacg tgccttgaag gtttacgata
     5101 gtcaggtttc aaaactacgc gatgaagaag cagttaaaga attccaaaaa gttttgattc
     5161 tacgagtggt ggataacaag tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg
     5221 cggttggact tcgtggctat gctcagaaca accctgttgt tgagtatcag gcagaaggtt
     5281 tccgtatgtt taatgatatg attggttcga ttgagtttga tgtgacacgc ttgatgatga
     5341 aagcacaaat tcatgaacaa gaaagaccac aggcagaacg tcatatcagt acaacagcga
     5401 ctcgcaatat cgctgctcac caagcaagta tgccagaaga tttggatttg agccagattg
     5461 gacgcaatga actttgccca tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa
     5521 gaaaataaaa tgagatagtt tagaggcgga tatcttgtga aaagtaaatt tttactgggt
     5581 atccgtttgc tttataagga gatgagttat ggtatttaca gcaaaaagct ctaaaataaa
     5641 tatagaagaa gttcgtgcct tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca
     5701 gcgagatcaa gagctagaag ccattatacg tggagaagac cagcgaattc tcttggtaat
     5761 cgggccatgc tcatctgaca acgaagaagc tgtccttgaa tacgctaagc gtttggcagt
     5821 cctacaagaa gaagtggcag atcgtatctt tatggttatg cgtgtttata ctgccaaacc
     5881 ccgtaccaac ggagatggct ataagggctt gattcaccag cctaacgcga cagaagcgcc
     5941 tagtcttatc aatggaatca aagccgttcg ccatcttcac tatcgtgtca tcacagaaac
     6001 agggatgaca actgctgatg aaatgcttta tcctgaaaac cttccgcttg tagatgattt
     6061 gatttcttac atggcagttg gtgcccgttc agttgaagac cagcaacacc gctttgtggc
     6121 aagtggggca ggattttcta ctggttttaa aaatccaacc tctggaaatc tcaatgtcat
     6181 gtttaatggg atttatgctg ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt
     6241 agaaacaact gggaacccgc tttcacacgc tattcttcgt ggtgctctta atgagtatgg
     6301 aaaaaatatt cccaactact attatgacaa tttaattgat accattgccc agtatgagaa
     6361 aatgggcttg gaaaatcctt ttatcatcat tgataccaat catgacaatt ctggtaagca
     6421 gtatattgaa cagatccgaa ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa
     6481 aattaagcag ttcgttcgtg gttttatgat tgagtcttat ctggaagatg gtcgacaaaa
     6541 tgagccagaa gtatttggta agtctatcac agacccttgc ctgggttggg ataacacaga
     6601 agctcttgtc agagaaattt acaaaacgtt aggagaataa gatggcattt attgaaaaag
     6661 gtcaagaaat cgatatggaa gtcattaagg ctgaaaccca attgtctgcg gaagccttga
     6721 gactcaagga aagccgtgac agggaattgg cagatattat ttcaggggaa gatgaccgta
     6781 ttctcttggt gattggtcct tgctcttctg ataatgaaga ggcggtcttg gaatatgctc
     6841 gccgtttatc cgccttgcaa aagaaggtag cggacaagat tttcatggtc atgcgcgtgt
     6901 atactgctaa gcctcgtacc aatggagacg gctataaagg attagttcac cagccagata
     6961 cttctaaggc tccaagcctg attaatggct tgcaggctgt gcgccagttg cactaccgcg
     7021 tgattacaga gactggtttg acaacggcag atgagatgct ttatccgtca aatctgatct
     7081 tggtggatga cttggtcagc taccatgccg ttggagctcg ttctgtggaa gaccaagagc
     7141 accgctttgt ggcttctggg attgatgcac cagtagggat gaaaaatcca acctcaggaa
     7201 atttgggtgt tatgtttaac gccatctatg ctgctcaaaa caagcaaacc ttcctttatc
     7261 atgggcagga agttgagaca tcaggtaatc ctttggccca tgttatcctc cgtggagcag
     7321 tcaacgagta tggcaattat atgccgaatt actactatga aaacctactc caagccattg
     7381 aacgctatga aaccatggga cttgaaaatc cttttatcct cattgacacc aaccatgata
     7441 actcaggcaa gcaatatatg gagcagattc gaattgttcg ccagaccttg cagaatcgtg
     7501 attggaatga gaaaattaaa aagacggttc gaggatttat gattgaatct tacctagcag
     7561 atggtcgtca aaaccaacca gagatctttg gttgctctat tactgaccct tgcctaggtt
     7621 gggaaaatac agaggccttg gtagaagaga tttatgttac cttgacaaaa taagtgaaaa
     7681 ggatggagtt ggggaatctc aactcctttt gatgagaatg atagttggac acggaattga
     7741 catcgaagaa ttggcttcga tagaaagcgc agttacacga catgaaggat ttgctaagcg
     7801 tgtactgacc gctcaggaaa tggagcgctt caccagtctc aaaggacgca ggcaaataga
     7861 atatttagct ggtcgctggt cggctaagga ggccttttcc aaggctatgg gaacgggcat
     7921 tagcaagctc ggttttcagg atttggaagt cttgaacaat gaacgtgggg cgccttattt
     7981 tagtcaggca ccattttcag gaaagatttg gctgtctatc agccacaccg atcagtttgt
     8041 gacagccagt gtcattttgg aggaaaatca tgaaagctag tccacataga ccaaccaagg
     8101 ttctgattca tctgggagct attcgacaaa atattcagca aatgggggct catatccctc
     8161 aaggaacgct caagtgggct gtggtcaagg ccaatgctta tggccatgga gctgttgccg
     8221 ttgccaaggc aattcaagat gatgttgatg gcttttgcgt ttccaatatc gatgaagcca
     8281 ttgaactcag acaagctgga ctcagcaagc caatcctcat tttaggagtt tctgaaatcg
     8341 aagctgttgc tctagctaaa gaatatgact tcaccttgac agtggctgga ctggagtgga
     8401 ttcaagcact cttagataag gaagtggacc taactggatt gacagtccac ctcaagattg
     8461 attcagggat gggacggatt ggttttagag aggccagtga ggttgagcag gctcaagatt
     8521 tgctacaaca acacggtgtt cgtgttgaag gaatctttac ccactttgct actgctgatg
     8581 aggaatcaga tgactatttt aatgcccagt tagaacggtt taaaactatt ttagctagta
     8641 tgaaggaagt tccagagctg gttcatgcta gcaattctgc aacgactctt tggcatgtag
     8701 agactatttt caatgcggtt cgtatgggag atgccatgta tggcctcaat ccaagtggag
     8761 cggtcttgga tttgccttat gatttgatac cggccttgac cttggagtct gctctggttc
     8821 atgtcaagac agttccagct ggagcttgca tgggctatgg agcaacttat caagcggata
     8881 gcgagcaagt catcgcgacc gtgccaatcg ggtatgcaga tggatggaca agagacatgc
     8941 aaaatttctc tgtcttggta gatggccaag cttgcccaat tgtcggcagg gtttcgatgg
     9001 accaaatcac tattcgattg cctaagcttt atccgctagg aaccaaggta accttgattg
     9061 gctccaatgg ggataaggaa atcactgcaa ctcaggtagc gacctaccgc gtaaccatta
     9121 actatgaggt ggtttgcctc ctcagcgacc gtattccgag agaatattat tagaaaagaa
     9181 aggagtggag catgaatcta catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt
     9241 cagcagaaaa atacgccaaa ctaggaattg aaaacttgca agatctcttg ctctactttc
     9301 ctttccgtta tgaagacttc aaaaccaagc aggtgctgga gctggaagac ggtgagaagg
     9361 cagttctttc tggtcaggta gtgactcctg ctagtgtcca gtattatggt ttcaagcgca
     9421 atcgcctgcg ttttagtctc aagcagggag aggtcgtttt tgcggtgaat ttctttaacc
     9481 agccctatct ggctgataaa atagagttgg gagcaaccct tgctgtcttt ggaaaatggg
     9541 accgcgctaa ggctagtctg actgggatga aggttctggc tcaggtagaa gatgacctcc
     9601 agcctgtcta tcgtctggct cagggaatca gtcaggccag tctggtcaag gtcatcaaga
     9661 cggcttttga tcagggactg gacctcttga tagaagaaaa tctgccccag tctttactag
     9721 acaaatacaa actcatgtcc cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt
     9781 tggcagaata caagcaggct cttcgccgta tcaagtttga ggaactcttt tatttccaaa
     9841 tgcagctgca gacgctcaag tctgaaaata gagttcaggg aagtggtctg gttctggatt
     9901 ggtctcagga aaaagtgaca gcagttaaag caagtcttcc ttttgccctg acccaagctc
     9961 aggaaaagag tttgcaggaa attttaactg atatgaagtc cgaccaccac atgaatcgtc
    10021 tcctacaagg agatgtgggg agcggaaaaa cggtagtcgc tggcttggcc atgtttgcgg
    10081 cagtgacagc aggttatcag gctgccctaa tggtaccaac agaaatcctc gcagagcaac
    10141 actttgagag tttacagaac ctttttccca atttgaaact ggctctcttg acaggttcct
    10201 tgaaagctgc agaaaagaga gaagtcttgg agaccattgc caagggtgag gctgatttga
    10261 ttataggaac tcacgctctg atacaagatg gggtggagta tgctcgtctt ggtttgatta
    10321 ttatcgatga gcagcaccgt tttggtgtag ggcaaaggcg tattttacgg gaaaaaggcg
    10381 acaatccaga tgtcctcatg atgacggcga ctcccattcc acggacgctt gccatcacag
    10441 cctttggaga tatggatgtt tccattatcg accagatgcc agcaggtcgg aagcctattg
    10501 tgacgcgctg gatcaaacat gagcaactac ctcaggtctt gacttggtta gagggggaaa
    10561 ttcaaaaagg ttcccaagtc tatgtcatct ctcctttgat tgaagaatca gaagctctag
    10621 atttgaaaaa tgccattgcc ttatcagagg agttgacgac tcattttgca ggcaagacag
    10681 aggtggctct tctacatggt aggatgaaga gtgacgaaaa agaccagatc atgcaggatt
    10741 tcaaggagag aaagacggat attctggttt cgacgacggt tattgaggtt ggggtcaacg
    10801 ttcccaatgc gactgtcatg attatcatgg atgccgatcg cttcggtctc agtcaacttc
    10861 accagcttag aggtcgtgtc ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcca
    10921 atcccaagac ggattctggg aaagaccgca tgcgtatcat gacagaaacg accaatggat
    10981 ttgtccttgc ggaggaagat ttgaaaatgc gtggttctgg tgagattttt ggaaccagac
    11041 agtcaggact tccagagttc caagtggctg atattatcga agattttccg attttagaag
    11101 aagcaagaaa ggttgctagc tacattagtt ctatagaagc ttggcaagaa gatccagagt
    11161 ggcgcatgat tgcccttcat ctggaaaaga aagaacatct ggattaagct ttctctaagg
    11221 aaaacttaag ctagttttta gatttggctc ttatactaaa gtcatcaaaa agaaacgagg
    11281 actctcatat gacagtaacg attaaagtaa attaccaaac cactttccaa aagaaggaag
    11341 caaaaaacta gtataaacag aagagagagc gaaatgctct ttttcgtttc taaaactact
    11401 ttcagcccat catcctaaaa gtaaagaatc taaattcact ttctatttat ccttctttct
    11461 tgcattgatt acatagatat gctacagttg tggtaacgat tacaaaataa aaggagcatg
    11521 ctatgaaaaa tccagcttta ctagaagaaa ttaagaccta tagaggaagg gatgaggttc
    11581 cggaagactt tgatgctttc tgggatgggg aagtgaaaaa tgtttccacg cttccatcct
    11641 accacttgga ggaaagagat ttccacattc ctcaagtcaa gtgctatgag ttaacatttg
    11701 aaggaagcaa ggaaggaaag gtctatgcac gcattgttct tccaaagagt gaggagaagg
    11761 ttccactaat cttccatttt catggttata tgggacgtgg ctgggactgg gccgacatgc
    11821 tgggcttcac cgtagctggt tacggtgttg tttccatgga tgtgcggggc cagtcaggtt
    11881 actcacaaga cggcttgcgt tctcctttag gaaataccgt gaaggggcat attatccgtg
    11941 gtgctatgga aggtcgggac cacctctttt ataaggatgt ttatctggat atttaccagt
    12001 tggtcgaaat tgttgctagt ctgtctcagg ttgatgagaa gcgtctttct agctatggtg
    12061 cctcacaaag gaggggctct agctctagtt gcagcagcgc tcaatcctcg aattcagaaa
    12121 acagttgcca tttatccctt cttgtcagac ttcagacggg tgattgagat tggtaatact
    12181 agcgaggctt acgacgaact tttccgttat ttcaagtttc acgacccctt ccatgaaaca
    12241 gaggaggaaa tcatggcgac ccttgcctat atcgatgtca aaaatcttgc ccatcgtatc
    12301 caaggtgagg ttaagatgat tacgggcttg gacgacgatg tttgctatcc cattacccag
    12361 tttgcgattt ataatcgtct gacctgcgat aaaacctatc gcatcatgcc tgagtatggt
    12421 cacgaagcca tgaatgtatt tgtcaatgac caagtctaca actggctctg tggaagtgag
    12481 attcctttta aatatctaaa ataaggagtc gactctaagc acaaaatctt aaaaattaca
    12541 aacacgcata gtatcagggg attaaaaaaa cttgatacta tgcgttttat catggaaata
    12601 tagtaaaatg aaacaagaat aggacaaaac gatcaggaca gtcaaatcga tttctaacaa
    12661 tgttttagaa gcaaatgtgt actattctag tttcaatcta ttatatttat agaatttttt
    12721 gttgctagat ttgtcaaatt gcttaaaata atttttttca gaaagcaaaa gccgatacct
    12781 atcgagtagg gtagttcttg ctatcgtcag gcttgtctgt aggtgttaat acttttcaaa
    12841 aatctcttta aaccacgtca gcttcgcctt gccgtaggta tggttactga cttcgtcagt
    12901 tctatccac
//
LOCUS       DAVHIU010000047        12525 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVHIU010000047 DAVHIU010000000
VERSION     DAVHIU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.049"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            46..276
                     /gene="blpU"
                     /locus_tag="VVN06_001165"
     CDS             46..276
                     /gene="blpU"
                     /locus_tag="VVN06_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU5562717.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            991..3144
                     /locus_tag="VVN06_001166"
     CDS             991..3144
                     /locus_tag="VVN06_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU5562718.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3157..4506
                     /locus_tag="VVN06_001167"
     CDS             3157..4506
                     /locus_tag="VVN06_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU5562719.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4676..5383
                     /locus_tag="VVN06_001168"
     CDS             4676..5383
                     /locus_tag="VVN06_001168"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5562720.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5440..9165
                     /locus_tag="VVN06_001169"
     CDS             5440..9165
                     /locus_tag="VVN06_001169"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU5562721.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELKAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9258..10700
                     /locus_tag="VVN06_001170"
     CDS             9258..10700
                     /locus_tag="VVN06_001170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5562722.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10737..11759
                     /locus_tag="VVN06_001171"
     CDS             10737..11759
                     /locus_tag="VVN06_001171"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5562723.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGLHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11756..12301
                     /gene="purN"
                     /locus_tag="VVN06_001172"
     CDS             11756..12301
                     /gene="purN"
                     /locus_tag="VVN06_001172"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5562724.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12385..>12525
                     /locus_tag="VVN06_001173"
     CDS             12385..>12525
                     /locus_tag="VVN06_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5562725.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIP"
BASE COUNT         3492 a         2396 c         3092 g         3545 t
ORIGIN      
        1 gtttgtaaca agacaaatat taaaaataaa aaagaggtat tcgttatgaa tacaaaaaag
       61 atgtcacaat ttgaaattat ggatactgag atgcttgctt gcgttgaagg tggcggatgc
      121 aattggggag attttgccaa agcaggtgtt ggaggaggag cagcacgagg tcttcagcta
      181 ggaattaaaa caagaacatg gcaaggtgca gcaactggtg ctgcgggagg agctatactt
      241 ggaggtgtgg cctatgcagc gacatgttgg tggtaattat ggattttaaa agttttatta
      301 ttggtttagt agttggtata tttggtcctt atatggatga tttaattaga aaaaaatttt
      361 taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa ataatcaaaa ctataaatga
      421 tgaatctgaa tcaaaattat tttgcgcatg taaagaggag tcttatagta acgagtcaaa
      481 aaaggagtaa ctatgaatcg taatttagaa cggtgttatc tattctgact aggaatagat
      541 cataccagag gtagcttaga aatagcagag acattagaaa ttgaagtaat aaataggatg
      601 tcgtaagtgt tactatcaat gatttatttg tttcaagctt gcctagggtg acagtaaaaa
      661 atcaatttcc tttcaatagc atatttttag tgggcaggac tcttgttctg cctatttttt
      721 tatccaaaaa gtgcagttgg gagggagata ggcgcatttg ggaaggaagt ccagtttttg
      781 tttagtgatt ggggtaagat agttgttatc agatgagttt atactcttcg aaaatcaaat
      841 tcaaaccacg tcaacgtcgc cttgccgtat atatgtgact gacttcgtca gtcctatcta
      901 caacctcaaa acagtgtttt gagcagcctg cggctagttt cctagtttgc tctttgattt
      961 tcattgagta ttagggaaaa ggagatgaat atgaaatttg ggaaacgtca ctatcgtccg
     1021 caagtggatc agatggactg cggtgtagct tcattagcca tggtttttgg ctactatggt
     1081 agttattatt ttttggctca cttgcgagaa ttggctaaga cgaccatgga tgggacgacg
     1141 gctttgggct tggtcaaggt ggcagaggag attggttttg agacgcgagc cattaaggcg
     1201 gatatgacgc tttttgactt gccggatttg acttttcctt ttgttgccca tgtgcttaag
     1261 gaagggaaat tgctccacta ctatgtggtg actgggcagg ataaggatag cattcatatt
     1321 gccgatccag atcccggggt gaagttgact aaactgccac gtgagcgttt tgaggaagaa
     1381 tggacaggag tgactctttt tatggcacct agtccagact ataagcctca taaggaacaa
     1441 aaaaatggtc tgctctcttt tatccctata ttagtgaagc agcgtggctt gattgccaat
     1501 atcgttttgg caacactctt ggtaaccgtg attaacattg tgggttctta ttatctgcag
     1561 tctatcattg atacctatgt gccagatcag atgcgttcga cactagggat tatttctatt
     1621 gggctagtca tcgtctacat cctccagcaa atcttgtctt acgctcagga gtatctcttg
     1681 cttgttttgg ggcaacgctt gtcgattgac gtgattttgt cctatatcaa gcatgttttt
     1741 cacctcccta tgtccttctt tgcgacacgc aggacagggg agatcgtgtc tcgttttaca
     1801 gatgctaaca gtatcatcga tgcgctggct tcgaccatcc tttcgatttt cctagatgtg
     1861 tcaacggttg tcattatttc ccttgttcta ttttcacaaa ataccaatct ctttttcatg
     1921 actttattgg cgcttcctat ctacacagtg attatctttg cctttatgaa gccgtttgaa
     1981 aagatgaatc gggataccat ggaagccaat gcggttctgt cttcttctat cattgaggac
     2041 atcaacggta ttgagactat caagtccttg accagtgaaa gtcagcgtta ccaaaaaatt
     2101 gacaaggaat ttgtggatta tctgaagaaa tcctttacct atagtcgagc agagagtcag
     2161 caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg tcggcattct ctggatgggg
     2221 gctgttctgg tcatggatgg caagatgagt ttggggcagt tgattaccta taataccttg
     2281 ctggtttact ttaccaatcc tttggaaaat atcatcaatc tgcaaaccaa gcttcagaca
     2341 gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag tagcttctga gtttgaggag
     2401 aagaaaacag ttgaggattt gagcttgatg aagggagata tgaccttcaa gcaggttcat
     2461 tacaagtatg gctatggtcg agacgtcttg tcggatatca atttaaccgt tccccaaggg
     2521 tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga cgactttggc caagatgatg
     2581 gttaattttt acgacccaag tcaaggggag attagtctgg gtggtgtcaa tctcaatcag
     2641 attgataaaa aagccctgcg ccagtacatc aactatctgc ctcaacagcc ctatgtcttt
     2701 aacggaacga ttttggagaa tcttcttttg ggagccaagg aggggacgac acaggaagat
     2761 atcttacggg cggtcgaatt ggcagagatt cgagaggata tcgagcgcat gccactgaat
     2821 taccagacag aattgacttc ggatggggca gggatttcag gtggtcaacg tcagagaatc
     2881 gctttggcgc gtgctctctt gacagatgcg ccggtcttga ttttggatga ggcgactagc
     2941 agtttggata ttttgacaga gaagcggatt gtcgataatc tcatggcttt ggacaagacc
     3001 ttgattttca ttgctcaccg cttgactatt gctgagcgga cagagaaggt agttgtcttg
     3061 gatcagggca agattgtcga agaaggaaag catgctgatt tgcttgcaca gggtggcttt
     3121 tacgcccatt tggtcaatag ctagaaagag gagaggatga aaccagaatt tttagaaagt
     3181 gcggagtttt ataatcgtcg ttaccataat ttttccagta gtgtgattgt acccatggcc
     3241 cttctgcttg tgtttttact tggctttgca actgttgcag agaaggagat gagtttgtcc
     3301 actagagcta ctgtcgaacc tagtcgtatc cttgcaaata tccagtcaac tagcaacaat
     3361 cgtattcttg tcaatcattt ggaagaaaat aagctggtta agaaggggga tcttttggtt
     3421 caataccaag aaggggcaga gggtgtccaa gcggagtcct atgccagtca gttggacatg
     3481 ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga gcctgcaaga aggggagaac
     3541 cactttccag aggaggataa gtttggctac caagccacct ttcgcgacta catcagtcaa
     3601 gcaggcagtc ttagggctag tacatcgcaa caaaatgaga ccatcgcgtc ccagaatgca
     3661 gcagctagcc aaacccaagc cgaaatcggc aacctcatca gtcaaacaga ggctaaaatt
     3721 cgcgattacc agacagctaa gtcagctatt gaaacaggtg cttccttggc cggtcagaat
     3781 ctagcctact ctctttacca gtcctacaag tctcagggcg aggaaaatcc ccaaactaag
     3841 gttcaggcag ttgcacaggt tgaagcacag atttctcagt tagaatctag tcttgctact
     3901 taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct atgcgtcagg gttaagcagt
     3961 caattggaat cccttaaatc ccaacacttg gcaaaggttg gtcaggaatt gacccttcta
     4021 gcccagaaaa ttttggaggc agagtcaggt aagaaggtac agggaaatct tttagacaag
     4081 gggaaagtta cggcgagtga ggatggggtg cttcatctta atcctgagac cagtgattct
     4141 agcatggttg cagaaggtgc cctactagcc caactttatc catctttgga aagagaaggg
     4201 aaagccaaac tcacagctta tctaagttca aaatatgtag caagaatcaa ggtcggtgat
     4261 tctgttcgct atactacgac tcatgatgcc gggaatcaac ttttcctaga ttctactatt
     4321 acaagtattg atgcgacagc tactaagact gagaaaggga atttctttaa aatcgaggcg
     4381 gagactaatc taacttcgga gcaggctgaa aaacttaggt acggggtgga aggccgcttg
     4441 cagatgatta cgggcaagaa aagttaccta cgttattatt tggatcaatt tttgaacaaa
     4501 gagtaatgtt cgtgttttta gagttaaata atttttaaac tgtgagaaag attcttcttg
     4561 cagttttttc tttacaattt ttgaaaaaca tctactattt attcggttaa attcttgtgt
     4621 tttttggttt tttgtggtaa aatgtgctca agtaatacga aaggcgaact ttaaaatgtc
     4681 aaaacaattg atctattcgg gaaaagctaa agatatctat acaactgagg atgaaaatct
     4741 tattatttca acttacaagg accaggcgac tgctttcaac ggtgtcaaga aggagcagat
     4801 tgcaggtaag ggagtcttga ataatcagat ctcatctttt atttttgaga aattaaatgt
     4861 ggctggtgtg gcgactcact ttgtggagaa actttcagac acggaacaac tcaataaaaa
     4921 ggttaagatt attcctttgg aagtcgtgct ccgcaactat actgctggtt ccttttcaaa
     4981 acgttttggt gtggatgagg gaatcgcctt ggagactccg attgtcgaat tttactacaa
     5041 aaatgatgat ttggatgatc catttatcaa tgatgagcat gtgaaattcc tacagattgc
     5101 gggtgaccag cagattgcct acttgaagga agaaacgcgt cgtatcaatg aactattgaa
     5161 agtctggttt gctgagattg ggcttaaatt gattgacttt aagctagagt tcggttttga
     5221 caaggatggc aagattatct tggcagacga attttcacca gataactgcc gcttgtggga
     5281 cgctgatggc aaccacatgg ataaggatgt tttccgtaga ggattgggag aactaaccga
     5341 cgtttatgag attgtttggg aaaagttgca gggtttaaaa taacaacctc aaggctgttt
     5401 gggaatattg caagagctga aataaaggaa taagaattga tggataaacg tatttttgtt
     5461 gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg ttagagagct ccagcacaac
     5521 ttgggactgt caagcttgaa aagtattcgt attgtgcaag tatatgatgt atttgacttg
     5581 gttgaggact tgtttgcacc tgcagagaag cacattttct ctgagcaggt aaccgaccat
     5641 gttttagatg aagtatctgt gcaggcggat cttgctaact atgctttctt tgccattgaa
     5701 agtctaccag ggcagtttga ccagcgtgca gcttcgtcac aggaagcctt gcttttgttg
     5761 ggaagttcga gtgacgtgac agtcaacaca gcccaacttt acttggtgaa taaagatatt
     5821 gatgcgactg agttgaaagc tgtcaaaaac tacctgctca atccagttga ttctcgtttc
     5881 aaggatatca cgacagggat tgccaagcag gagttttcag agtcagacaa gaccattcca
     5941 aaattgactt tctttgaaag ctatacagca gaagattttg ctcgctacaa ggccgaacaa
     6001 gggatggcca tggaagtgga tgatttgctc tttatccaag actactttaa gtcaatcggg
     6061 cgcgtgccaa ctgagactga acttaaggtt ttggacactt actggtctga ccactgccgt
     6121 catacgactt ttgagacaga gttgaaacac atcgactttt cagcttctaa atttcaaaag
     6181 caattgcagt caacctatga caagtatatt gccatgcgcg aggaattagg tcggtctgaa
     6241 aaaccacaaa ccttgatgga tatggcgact attttcggtc gttatgagcg tgctaatgga
     6301 cgattggatg atatggaagt ctctgacgaa atcaatgcct gctcagttga aattgaagtg
     6361 gacgttgatg gtgtcaagga accttggctc ctcatgttta aaaacgaaac ccacaaccat
     6421 ccaacagaaa ttgagccatt tggtggagcg gctacctgta ttggtggagc tattcgtgat
     6481 ccgttgtcag gccgttccta tgtttaccaa gccatgcgta tttcaggtgc tggtgatatt
     6541 acagcaccga tttcggaaac tcgcgctggg aaattgccac aacaagtcat ttctaaaaca
     6601 gcagctcatg gttattcttc atatggtaac cagattgggc ttgccacaac ctacgttcgt
     6661 gaatacttcc acccaggctt tgtagctaaa cgtatggaac ttggtgccgt tgttggtgcg
     6721 gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag gtgatgtgat catccttctc
     6781 ggaggtaaaa caggtcgtga tggtgtcggt ggtgcgacgg gctcttctaa ggttcaaaca
     6841 gttgagtctg tagagactgc tggtgctgag gttcaaaaag gaaatgccat cgaagaacgc
     6901 aagattcagc gcctcttccg taatggcaat gtcactcgtc tgatcaagaa gtccaatgac
     6961 tttggggcag gcggtgtctg tgtggctatc ggtgaattgg cagacggtct tgaaatcgac
     7021 ctcaacaagg tgcctcttaa ataccagggc ttgaatggta cagaaattgc catctctgaa
     7081 tcacaagaac ggatggcggt cgtggttcgt cctgaagatg tggatacctt cgttgccgaa
     7141 tgtaacaaag aaaatattga tgctgttgtg gtggcgacag taactgaaaa accaaatctt
     7201 gtcatgcact ggaatggtga gacaatcgtt gacttggagc gtcgtttcct tgacaccaat
     7261 ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg atgtcaaact cccagaagag
     7321 cgtcaaacat ctgctgaaac actggaatca gataccctta cggttctatc tgacctcaac
     7381 catgcaagtc aaaaaggatt acagactatc tttgactgct ctgttggacg ctcaacggtt
     7441 aatcacccac ttggtggtcg ttaccaactc acaccaactg aggcatctgt gcagaaattg
     7501 ccagttcaac acggtgtgac tcatactgcg tcggtcattg ctcaaggttt caacccatat
     7561 gtagctgaat ggtctccata ccacggtgct gcttatgcgg ttatcgaagc aactgctcgt
     7621 ttggtggctg ctggtgccaa ctggtctaag gctcgtttct cttaccaaga atatttcgag
     7681 cggatggaca agcaagcaga gcgttttggt cagccagtag ccgcccttct aggctctatt
     7741 gaagcacaaa ttcagcttgg cttgccatct atcggtggta aggactccat gtctggtacc
     7801 tttgaagaat tgaccgttcc gccaaccttg gttgcctttg gggtgacgac ggcagatagc
     7861 cgtaaggtgc tctctccaga atttaaagct gttggggaaa atatctacta catcccaggt
     7921 caagccctct ctgcagagat tgattttgac ttgattaaga aaaattttgc tcagtttgaa
     7981 gcaagccaag ctgaccataa agtgacatct gcatcagctg tcaaatacgg tggtgtagtt
     8041 gaaagtttgg ctcttgctac ctttggaaac tatattggtg cagaggtgac cttgcctgaa
     8101 cttaaaacag ctttgacagc tcaattaggc ggctttgtct tcacatctcc tgaagaaatt
     8161 gctggagtag agaagattgg acaaacgaaa gcagacttta cactgactgt caacggtgtg
     8221 aagctagatg gacacaagct tgacagtgca tttcaaggga cattggaaga agtttaccca
     8281 acagaattta cccaagcgaa agaactagaa gaagtaccag ctgtggtatc agatgttgtg
     8341 attaaagcca aagaaaaggt tgaaaaacct gtggtttaca tcccagtctt tccaggaacc
     8401 aactcagaat atgattcagc taaggccttc gaaaaagaag gtgcagaggt caatttggtg
     8461 ccattcgtga ccttgaatga agaagctatt gtcaagtcag ttgaaactat ggttgacaat
     8521 atcgacaaga ctaatattct cttctttgcc ggtggattct cggctgcgga tgaaccagat
     8581 ggttcagcta agtttatcgt caatatcctg cttaatgaaa aagtgcgtgt ggctattgat
     8641 agctttatcg cccgtggtgg cttgattatc ggtatttgta atggattcca agccttagtc
     8701 aaatcgggtc tcctaccata cggaaacttt gaagatgcta acagtactag tccaaccctc
     8761 ttctacaatg atgccaacca acacgtggcc aagatggtgg aaactcgtat tgccaatacc
     8821 aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc acgctattcc tgtttcgcac
     8881 ggtgaaggga agtttgtcgt gacggctgag gaatttgcag agctccgtga caatggacaa
     8941 attttcagcc aatacgttga ctttaacggt aaaccaagta tggattctaa gtacaatccg
     9001 aatggttctg tccatgccat cgaaggaatt accagcaaga atggtcaaat catcggtaag
     9061 atgggccact cagaacgtta tgaggatggt cttttccaaa atatcccagg caataaagac
     9121 caacacctgt tcgcatcagc ggttaaacat ttcactggaa aataagactt acagattttc
     9181 taatagatag tatcagtaat gtaaaagtca tgtaaatcta gctcttgatg attacaaatg
     9241 aaaattaggt ataaaaaatg acatacgaag taaaatctct taatgaagaa tgtggtgttt
     9301 tcggtatttg gggacatcca gatgctgcta agttgaccta ttttggactc cacagtcttc
     9361 aacaccgtgg tcaggagggg gcaggaatcc tctccaatga tcaaggacaa ctgaagcgcc
     9421 atcgtgacat ggggctttta tcagaagttt tcagaaatcc agccaatttg gataaattga
     9481 caggagctgg tgcgattggg catgtgcgtt atgcgactgc tggcgaagct tctgttgata
     9541 acatccagcc cttcctcttc cgttttcacg atatgcagtt tggcttggct cataatggaa
     9601 atctgaccaa tgcagcctct ctcaagaaag aactggaaca aagaggagca attttcagcg
     9661 cgacttcgga ctctgaaatc ttggctcacc tcattcgtcg cagtcataat cctagcctga
     9721 tgggtaaaat caaggaagcg ctcagccttg tcaaaggtgg ttttgcctat atcttgctgt
     9781 ttgaggacaa gttgattgcg gctcttgacc ctaatggctt tcgtccgctt tctatcggga
     9841 aaatggccaa cggagcggtg gttgtttcct ctgaaacctg tgcttttgag gtcattggtg
     9901 ccgagtggat tcgtgatttg aagccaggtg agattgtgat cattgatgac gagggcattc
     9961 agtatgacag ctatacagat gatactcagt tggcgatttg ttctatggag tatatctact
    10021 ttgctcgccc tgattctaat atccacggtg tcaatgtcca tacggcacgt aagagaatgg
    10081 gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat tgtagttggt gtgcccaatt
    10141 cttccctaag cgcggctatg ggatttgcgg aagaatcagg cttaccaaat gaaatgggtc
    10201 tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc gactcaagaa ttgcgggagc
    10261 aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt caaaggcaaa cgtgtggtca
    10321 tggtggatga ttccattgta cgtggaacaa cctctcgtcg tatcgttcag ctcttgaaag
    10381 aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc tgcactagcg tatccatgtt
    10441 tctacgggat tgatatccag acccgtcagg agctgattgc agccaatcat acggtcgaag
    10501 aaactcgcca aatcattggt gcggacagtc tgacttatct ttcaattgat ggcttgattg
    10561 agtcgattgg tatcgaaaca gatgcgccga acggtggtct ctgtgtcgct tactttgacg
    10621 gtgactaccc aacgcctctc tatgactacg aagaagacta tcgtagaagt ttggaagaaa
    10681 agaccagttt ttacaagtag gcgacagatt ctccattaaa gaaaaggaaa aaacaaatga
    10741 caaataaaaa tgcatatgcc cagtcgggtg tggatgttga agcgggttat gaagttgttg
    10801 aacggattaa aaagcacgtg gcccgtacgg agcgtgcagg tgtcatggga gctcttggtg
    10861 gctttggtgg tatgtttgac ctttccaaga ctggggttaa agaacccgtc ttgatttcag
    10921 ggactgacgg tgtcggaacc aagctcatgt tggctatcaa gtacgacaag cacgatacca
    10981 tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt gcggaacccc
    11041 tctattttct cgactacgta gcgacaggga agaatgagcc agctaagcta gaacaagtgg
    11101 ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt ggggaaacgg
    11161 ctgaaatgcc gggcatgtac ggcgaagacg actatgactt ggctggtttt gcggtcggtg
    11221 tggctgaaaa atctcaaatc attgacggtt caaaggtggt agagggagac gttcttctcg
    11281 gacttgcttc aagtgggctt cactcaaatg gttactcttt ggttcgtcgt gtctttgcgg
    11341 attacacagg tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag gaagttctac
    11401 ttgagccgac tcgtatctat gtcaaggctg tcttgccgct catcaaagaa gagttggtca
    11461 acggcattgc ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt atgtttgcag
    11521 atgacctagc tgctgaaatt gatgaaagca aagttccagt gcttccaatt ttcaaagccc
    11581 ttgaaaaata tggtcagatt aaacacgaag aaatgtttga aatcttcaat atgggtgtgg
    11641 gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg ttggatgaag
    11701 cagtctatga aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt atcaaatgaa
    11761 aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt caggtgattg ccgaagaatt
    11821 tccagtggag tttgtctttt cagaccatcg tgatgcctat gtgcttgagc gtgcaaagca
    11881 gctcggcgtt ctgtcctatg cttttgaact caaggagttt gagagcaagg cagactacga
    11941 agcagccctt gtcgaactct tggaagaaca ccagattgac ttggtttgcc tagcaggcta
    12001 catgaaaatc gttggaccaa ccttattgtc ggcttatgaa ggtcggattg tcaacattca
    12061 tccagcctac ttgccagaat ttccaggagc tcatgggatt gaggatgctt ggaatgctgg
    12121 cgtgggtcag tctggtgtga ccattcactg ggtggattcg ggtgtggata caggccaggt
    12181 catcaaacag gttcgtgtgc cacgactagc tgatgatacc attgacagat ttgaagctcg
    12241 catccatgaa gcagagtaca ggttgtatcc ggaagtagtg aaggctctat ttacagattg
    12301 actttttgat gattcatatg atatctttga ttttaaattg gagtcagtgt ttgttgaaga
    12361 cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg atatgtattt
    12421 tatgtctatc tgatgttatt aacttgggga atcttattta agtttgaaac aaatcctgaa
    12481 tttatagcat ttttcttagc tccaaggtat atcaattgga ttcca
//
LOCUS       DAVHIU010000048        12363 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVHIU010000048 DAVHIU010000000
VERSION     DAVHIU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.050"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(77..1744)
                     /locus_tag="VVN06_001174"
     CDS             complement(77..1744)
                     /locus_tag="VVN06_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU5562726.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1747..2112)
                     /locus_tag="VVN06_001175"
     CDS             complement(1747..2112)
                     /locus_tag="VVN06_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5562727.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2269..2457)
                     /locus_tag="VVN06_001176"
     CDS             complement(2269..2457)
                     /locus_tag="VVN06_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5562728.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2573..2749)
                     /locus_tag="VVN06_001177"
     CDS             complement(2573..2749)
                     /locus_tag="VVN06_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU5562729.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(2860..3058)
                     /locus_tag="VVN06_001178"
                     /pseudo
     CDS             complement(2860..3058)
                     /locus_tag="VVN06_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            complement(3485..5029)
                     /locus_tag="VVN06_001179"
     CDS             complement(3485..5029)
                     /locus_tag="VVN06_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU5562730.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5242..5544
                     /gene="gatC"
                     /locus_tag="VVN06_001180"
     CDS             5242..5544
                     /gene="gatC"
                     /locus_tag="VVN06_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5562731.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            5544..7010
                     /gene="gatA"
                     /locus_tag="VVN06_001181"
     CDS             5544..7010
                     /gene="gatA"
                     /locus_tag="VVN06_001181"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5562732.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            7010..8452
                     /gene="gatB"
                     /locus_tag="VVN06_001182"
     CDS             7010..8452
                     /gene="gatB"
                     /locus_tag="VVN06_001182"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5562733.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8576..9619
                     /locus_tag="VVN06_001183"
     CDS             8576..9619
                     /locus_tag="VVN06_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU5562734.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEDFIELT
                     QDKSQIKILVEPE"
     gene            complement(9755..9838)
                     /locus_tag="VVN06_001184"
                     /pseudo
     CDS             complement(9755..9838)
                     /locus_tag="VVN06_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9997..10557
                     /gene="efp"
                     /locus_tag="VVN06_001185"
     CDS             9997..10557
                     /gene="efp"
                     /locus_tag="VVN06_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5562735.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            10579..10968
                     /locus_tag="VVN06_001186"
     CDS             10579..10968
                     /locus_tag="VVN06_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5562736.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            10961..11383
                     /gene="nusB"
                     /locus_tag="VVN06_001187"
     CDS             10961..11383
                     /gene="nusB"
                     /locus_tag="VVN06_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5562737.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(11433..11669)
                     /locus_tag="VVN06_001188"
     CDS             complement(11433..11669)
                     /locus_tag="VVN06_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562738.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(12151..12336)
                     /gene="briC"
                     /locus_tag="VVN06_001189"
     CDS             complement(12151..12336)
                     /gene="briC"
                     /locus_tag="VVN06_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU5562739.1"
                     /translation="MTGTETFTVISTEDLEQTSSGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
BASE COUNT         3664 a         2738 c         2382 g         3579 t
ORIGIN      
        1 tttgttttta ttaaaaagat accgatttca tttactaaaa agaaaaaaga actgatttct
       61 cagtccttca ttaatcttat tccacactaa ataggtatgg gtaaacaggt tgttgacctt
      121 ggtgaatctc gacttcaacg tcttcgaatt cttctacgat ttcttgagcg atttcattgg
      181 caagttcttc gcttccgtct tcacctacat agaaggttac gatttcactg tcttcatcca
      241 acatatgttt caaggtttca gtcaatgttt ggtgcatatc agggtttgac acaagaattt
      301 ttccatccac catacctaaa ttatcgtttt catggatttc taagccatcg atcgttgtat
      361 cacgcacggc tgttgtgacg cttccgctaa cgacatcgct aagagcagct gtcatacgct
      421 cttggttttc ttcaatggac ttgcttggat caaaggcaag aagacttgtc ataccttgag
      481 gaagagtgcg agcctctacc actaccgctg gttgctccaa aacttctgcc gcagattgag
      541 ctgccatgaa gatgttcttg ttgtttggca agaagatgat gttacgggca ttaacctgtt
      601 caacagcctt gataaagtct tctgttgaag ggttcatggt ttgaccgcct tcgataacat
      661 aatccacgcc ttgagaacag aagatatctg ctagaccttt accagccacc acagcaatca
      721 aagcatactc tttttcttca gccgacttga taacttgagt agcttctttc tcaacctgtg
      781 cttcgtgttg gttacgcata ttgtcaactt ttaccttgac caagctacca tatttgagac
      841 cttcttgcat aacaagtcct ggatcttctg tatgaacatg gactttgaca atttcatcat
      901 cgttaacaac gaggagagaa tctccaagct catccaagta gttacggaat tcatcgtagt
      961 caaaatcttt agcataggtt ggaccttgct taagagctac catgatttca gtacagtaac
     1021 caaacgtgat gtcctcagtc gctacgtgac cagctacaga cttatgatgc tctacattga
     1081 tcatctcact catgttggca ggagtcgcta caaagtcctc agatgcaata tattcgccag
     1141 taagggctga aaggaaacct tcgtagatga agaccaatcc ttgaccacct gagtccacaa
     1201 cgccaacttc tttcaatact ggaagcatgt ctggtgtttt agctagagct gttttagcac
     1261 cttccaaggc tgcgcgcatg acttcaacag cgtcatctgt ttgctcagct tttttcttag
     1321 caccgatagc agctccacga gaaactgtta aaatcgttcc ttcaacaggt ttcatcactg
     1381 ccttataggc aacttccaca cctgattgga aggccagagc caagtcttga cctgttaact
     1441 cgtctttatc cttgatagct tgggaaaatc cacggaaaag ctgagacgta atcactcctg
     1501 agttcccacg cgcacccatc aaaagccctt tggcaagaat gctcgctact tctccaactg
     1561 tagaagctgg cttgtctgca acttctttag caccattttc aatggtcatt cccatatttg
     1621 tcccagtatc tccatctgga actggaaaga cgtttaatga attgacatat tcagcttgct
     1681 tattcaagcg agttgatgca gcctgcacca tttcttgaaa taagctagta gtaatttttg
     1741 acacgattat tctcctacaa ctttgatatt ttgaatgtag acatttacag tctgagcagt
     1801 aattccaagc tggttttcca agctaaaggc aacacgctct tgaatgtttt ttgacacttc
     1861 actaatcttt gttccgtagc ttaacacggt atatacatca actgcaatac tgccatcttc
     1921 ggctgccttt acgacgacac ctttagaata attttcctta cctagcaggg cttggaaatt
     1981 atctttgagg gcatttttac tagccatacc gaccacacca aaaatctcag ttgctgctcc
     2041 acctacgacg gttgcaatca cttcatctgt tagttcgatt tgaccatctt ttgtattaat
     2101 ttttacagtc atccttttta cctcaactag ttgatactct attttatcat atttcagccc
     2161 aagtgtaaaa gcagaatgat gtatcagcgg atatttactc tatttttcaa acgatgttat
     2221 acccacaata aaagaaaaaa gaccctaagg tctcctttgc ttttattatt aaacgcgttc
     2281 aactttacct gatttcaaag cacgagctga agcccaaact tttttaggtt taccatcgat
     2341 aagaacagta actttttgaa ggtttggttt tacggcacgt tttgtttggt tcatcgcgtg
     2401 tgaacggttg tttcctgata cagtcttacg acctgtaaag taacatactt tagccattgt
     2461 gttttcctcc tattagatct aatatagcgg atgtgctagc accacatacc gtactatgtt
     2521 atcacacttt cttgtttttt gcaagggaat tggaagattt tttatttgtg tcttaaatca
     2581 ggtcttgcgt gacattttct gctctccaca tgccattgtt gattaacaga acaccagaat
     2641 taaaattatg tgtataaaaa tcatctctaa ctgcagctaa gggtatagcc gtcaagtcca
     2701 aatcccacag ctcatctatc gattttctta caacaatatc taaatccaaa tacagtacac
     2761 gagactcgct tacatacttt ggaataaaat atctaaaaaa gccgcatatg aaagtccctc
     2821 aaaggggaga cgataacctt tcagaatact actgtcaatc taaacattca caatctcact
     2881 attcaaagtc tctagtcttt tttccatcaa ttggaaccat tctcgcggaa ggtcatcatt
     2941 aaaaacataa aacttaagat tataatagat gaacacaaag agattttatt gttgtttcaa
     3001 ctttatccat ataagcatta tctgcaccta agacaatcgc ttttttctct tctttcactt
     3061 tttatctcat ttctttttat tcccatcata tgtttctacg taaccattat tttcgcctat
     3121 tcgttcgtaa aaccatacca gtggagattt tagatgaagt cccattacgg tttacaattt
     3181 ttacattacg acacggagtt ttacaaatcg atttcatttg ccaaacgtag ttagtaaggc
     3241 agttagctag gctacctact ttctgctacg acggaaaaat ccacaaatca ataccattcg
     3301 tggatttttc ctagtgaagc gtttaggtaa gccgccatag cggttaccga ttattagatt
     3361 acgacacgga gtttggcaaa tcgattctat ttgccaaacg tagttagtga ggcagttagc
     3421 tagttcgcca aatagcgact agcgtccaac aattggaact ttagttccaa ttgttggtac
     3481 tgagtcacat cttctcctct aactctacgt ctggatactt gtccgcaaac cagcggaggg
     3541 caaagtcatt ttcaaagaga aagactggtt ggtcaaaacg gtctttggct aagatattgc
     3601 gacttgacga catccgttca tccaagtcct caggcttgat ccaacgaacg gtctttttac
     3661 ccattgggct catgaccact tccgcattgt attcgccttc catacggtgt ttaaagactt
     3721 caaactggag ttgaccaaca gctcccagca tgtactcacc tgtttggtaa ttcttataaa
     3781 gctgaacggc tccttcttgc accaattgct caatcccctt gtggaaggat ttttgcttca
     3841 taacattctt agcagaaact ttcatgaaaa tttcaggagt aaaggttggc agtggttcaa
     3901 attcaaactt gttttttcca accgtcaagg tatccccaac ctgataagta ccggtatcgt
     3961 aaaccccgat aatatcacct gccacggcat tgatcacatt ctcacgactt tccgccataa
     4021 actgggtaac attagatagt ttagccccct taccagtacg agggagattg acactcatgc
     4081 cgcgctcaaa ttcgcctgat acgatacgga caaaggcaat acggtcacgg tgacgaggat
     4141 ccatgttggc ttggatttta aagacaaagc ctgagaaatc cttgtcataa ggatccacaa
     4201 tttcaccgtc tgttttcttg tgaccatgtg gttctggagc aaacttgagg aagatttcaa
     4261 ggaaggtctg cacaccaaag tttgtcaggg ctgaaccgaa aaagacaggc gtcaattctc
     4321 cagccagaat agcttcctct gaaaactcat tcccagcttc atttaaaagc tcaatgtcat
     4381 ccttgacttg ctcgtagaaa ggattgctac caaagagttt gtccccgtct tctagactgg
     4441 caaaacgctc atcccctttg taaagctcta aacgttggtt atagaggtca tacaagccct
     4501 caaaggcttt ccccatcccg ataggccagt tcatagggta gctagcaatg cccaagattt
     4561 cttccaattc ttgcaagaga tccaaaggct cacgaccgtc acggtccagc ttgttcataa
     4621 aggtaaaaac tggaatgcca cgatgtttca caacctcaaa caatttcttg gtttgagcct
     4681 cgatcccctt ggcagagtcc acgaccatga ccgcagcatc caccgccatc aaggtacgat
     4741 aggtatcttc tgagaagtcc tcgtgccctg gcgtgtctaa gatattcacg cgcttgccgt
     4801 cgtagtcaaa ttgcataaca gatgaagtaa cagaaatccc acgttgcttc tcgatatcca
     4861 tccagtcaga tttagcaaaa gtccctgttt tcttcccttt taccgtacca gcctcacgaa
     4921 tctcaccccc aaagtagagt aactgctcag tgatggttgt tttccccgcg tccgggtggg
     4981 agataatggc aaaggtacga cgtttcttaa tttcttcttg aatattcata agttctcttt
     5041 ctttgattct ctatttttct tgtttcaata gctgagaatg atttttacat tggattttac
     5101 cattcctttc aacactccat tatatcggat tttagcattt ttttcaattt ctatttcttt
     5161 tcacttcccc ctcccttatt tataggaaaa tatggtaaaa tagaacagac taaaaatcat
     5221 catttcacga aaggatgcaa gatgaaaatt acgcaagaag aggtaacaca cgttgccaat
     5281 ctttcaaaat taagattctc tgaagaagaa actgctgcct ttgcgaccac cttgtctaag
     5341 attgttgaca tggttgaatt gctgggcgaa gttgacacaa ctggtgtcgc acctactacg
     5401 actatggctg accgcaagac tgtactccgc cctgatgtgg ccgaagaagg aatagaccgt
     5461 gatcgcttgt ttaaaaacgt acctgaaaaa gacaactact atatcaaggt gccagctatc
     5521 ctagacaatg gaggagatgc ctaatgactt ttaacaataa aactattgaa gagttgcaca
     5581 atctccttgt ctctaaggaa atttctgcaa cagaattgac ccaagcaaca cttgaaaata
     5641 tcaagtctcg tgaggaagcc ctcaattcat ttgtcaccat cgctgaggag caagctcttg
     5701 ttcaagctaa agccattgat gaagctggaa ttgatgctga caatgtcctt tcaggaattc
     5761 cacttgctgt taaggataac atctctacag acggtattct cacaactgct gcctcaaaaa
     5821 tgctctacaa ctatgagcca atctttgatg cgacagctgt tgccaatgca aaaaccaagg
     5881 gcatgattgt cgttggaaag accaacatgg acgaatttgc tatgggtggt tcaggtgaaa
     5941 cttcacacta cggagcaact aaaaacgctt gggaccacag caaggttcct ggtgggtcat
     6001 caagtggttc tgccgcagct gtagcctcag gacaagttcg cttgtcactt ggttctgata
     6061 ctggtggttc catccgccaa cctgctgcct tcaacggaat cgttggtctc aaaccaacct
     6121 acggaacagt ttcacgtttc ggtctcattg cctttggtag ctcattagac cagattggac
     6181 cttttgctcc tactgttaag gaaaatgccc tcttgctcaa cgctattgcc agcgaagatg
     6241 ctaaagactc tacttctgct cctgtccgca tcgccgactt tacttcaaaa atcggccaag
     6301 acatcaaggg tatgaaaatc gctttgccta aggaatacct cggtgaagga attaacccag
     6361 aggttaagga aaccattcta aatgccgcta aacactttga aaaattgggt gctattgtcg
     6421 aagaagtcag ccttcctcac tctaaatacg gagttgccgt atactacatc atcgcttcat
     6481 cagaagcttc atcaaacttg caacgctttg acggtatccg ttacggctat cgcgcagaag
     6541 atgcaaccaa ccttgatgaa atctatgtaa acagccgaag ccaaggtttt ggtgaagaag
     6601 tgaagcgccg tatcatgctg ggtactttca gtctttcatc aggttactac gatgcctact
     6661 ataagaaggc tggacaggtc cgtacactta tcattcaaga tttcgaaaaa gtcttcgcgg
     6721 attacgattt gattttgggt ccaactgctc caagtgttgc ctatgacttg gattctctca
     6781 accatgaccc agttgccatg tacttagccg acctattgac catacctgta aacttggcag
     6841 gactgcctgg aatttcgatt cctgctggat tctctcaagg tctacctgtc ggtctgcaat
     6901 tgattggtcc caagcactct gaggaaacca tttaccaagt tgctgctgcc tttgaagcaa
     6961 caacaggcta ccacaaacaa caacccgtga tttttggagg tgacaactaa tgaactttga
     7021 aacagtcatc ggacttgaag tccacgtaga gctcaacacc aattcaaaaa tcttctcacc
     7081 tacttctgcc cacttcggaa atgatcaaaa tgccaacact aacgtgattg actggtcttt
     7141 cccaggagtt ctgccagttc tcaataaagg ggttgtcgat gccggaatca aggctgctct
     7201 tgccctcaac atggacatcc acaaaaagat gcactttgac cgcaagaact acttctaccc
     7261 tgataatccc aaagcctacc aaatttctca gtttgatgag ccaatcggtt ataacggctg
     7321 gattgaagtc gaactagaag acggtacgac caagaaaatc ggtatcgaac gtgcccacct
     7381 agaggaagac gctggtaaaa acacccatgg tacagatggc tactcttatg ttgacctcaa
     7441 ccgccaaggg gttcccttga ttgagattgt atctgaggca gatatgcgtt ctcctgaaga
     7501 agcctatgct tatctgacag ccctcaagga agttatccag tacgctggca tttctgacgt
     7561 taagatggag gaaggttcga tgcgtgtgga tgccaacatc tcccttcgtc cttatggtca
     7621 agagaaattc ggtaccaaga ctgaattgaa gaacctcaac tccttctcaa acgttcgtaa
     7681 aggtcttgaa tacgaagtcc aacgccaggc tgaaattctt cgctcaggtg gtcaaatccg
     7741 ccaagaaaca cgccgttacg atgaagcgaa taaagcaacc atcctcatgc gtgtcaagga
     7801 aggggctgct gactaccgct acttcccaga accagaccta cccctctttg aaatttctga
     7861 cgagtggatt gaggaaatgc ggactgagtt gccagagttt ccaaaagaac gtcgtgcgcg
     7921 ttatgtatct gaccttggtt tatcagacta cgatgctagt cagttgactg ctaataaagt
     7981 cacttctgac ttctttgaaa aagctgttgc cctaggtggt gatgccaaac aagtctctaa
     8041 ctggctccaa ggggaagtcg ctcagttctt gaatgctgaa ggtaaaacac tggaacaaat
     8101 cgaattgaca ccagaaaact tggttgaaat gattgccatc atcgaagacg gtactatttc
     8161 atctaagatt gccaagaaag tctttgtcca tctagctaaa aatggcggtg gcgcgcgtga
     8221 atacgtggaa aaagcaggta tggttcaaat ttcagatcca gctgttttga ttccaattat
     8281 ccaccaagtc tttgcggata acgaagctgc tgttgccgac ttcaagtcag gcaaacgtaa
     8341 tgcagacaag gccttcacag gattccttat gaaggcaacc aaaggccaag ccaacccaca
     8401 agttgccctt aaactacttg cacaggaatt ggcgaagttg aaagaaaact agacagaaca
     8461 aaaccagccc taaggttggt tttttcttga caaatagaaa gaaaacgttt gcaatattgc
     8521 gtgtaaagtt tccaaacaag aaaactaaaa atcgcaacaa taaggagtta tttatatggc
     8581 tacaatgaag gcagctcgct ggcatgcagc gaaagatgtt cgtattcaag aaatagaagt
     8641 tcctgaagta cttccgcacc aagtaaaagt tgcagttaag ttcacaggaa tttgtggtac
     8701 tgacctccat gaatttttag atggacctat ctttatccca acagatgagc acgtctattc
     8761 tggtcaaaaa gcaccggtaa cactgggaca tgaattttct ggtgaaatca tagaagtcgg
     8821 aagcgatgtt acccgtgtta aagttggtga tcgtgttgct gtggaaccaa ttctagctaa
     8881 aaataactta gttggtgatt ataatttgga tccaaacctt aattttgtcg gtttggctgc
     8941 agacggtgga tttgccaaat attgtgtttt agacggtgac ttagtacatg tcattccaga
     9001 tagtttgagc tatgagcaag ctgctcttac tgaacccgct gctgttgcag tctatgctgt
     9061 tcgtcaatct tctttgaagg ctggcgatac agctgttgtc tttggtttag gcccgattgg
     9121 tctattaatt gtagaagctc ttcgtgcagc tggtgcttct aagatttatg ctgttgaact
     9181 atcacctgaa cgtcaagcaa aagctgaaga gttaggagct atcgttgtcc gtccggaaga
     9241 aggtgaaaca gctgttgaag ctattcatcg tttaacaaac ggtggtgtag atgtttctta
     9301 tgaagtaacg ggtgtgccag tagttttagg acaagcctta gctgccgtac ataaagcagg
     9361 tgagtgcatg gtggtctcta tctgggaacg agaagcaaat attaatccga atgaatttgc
     9421 aatccaagaa aagacactaa aaggaatcat tgcttatcgt catatcttcc ctaaagtatt
     9481 agaattgatg gaacaaggct acttctctgc tgaaaaatta gttactaaga aaattaaatt
     9541 ggaaaatatc gtcgaagaag actttatcga attaactcaa gataagtcac aaattaaaat
     9601 tttggtagaa ccagaataaa aaatcaatct ttttaagaca atactaacag gtcggagtaa
     9661 acttacttcg gccttttgaa atataatact cttcgaaaat ctcttcagac cacgtcagcg
     9721 tcacattacc gtatatatgg ttactgactt cgtcagttct atccacaacc tcaaaacagt
     9781 gttttgagca gcctgcggct agcttcctag tttgctcttt gatttccatt gagtatgaga
     9841 agaaatatag tggatactct ttaacattaa ccactccacc tgactagcca atattctttt
     9901 tcgctcctac caactcccaa taactatttt ggctttattt ccagagtatt ttatggtaaa
     9961 atgaagagta ataatattta ttaaagaggt aaaaacatga ttgaagcaag taaattaaaa
    10021 gctggtatga cctttgaaac agctgacggc aaattgattc gcgttttgga agctagtcac
    10081 cacaaaccag gtaaaggaaa cacgatcatg cgtatgaaat tgcgtgatgt ccgtactggt
    10141 tctacatttg acacaagcta ccgtccagag gaaaaatttg aacaagctat tatcgagact
    10201 gtcccagctc aatacttgta caaaatggat gacacagcat acttcatgaa tacagaaact
    10261 tatgaccaat acgaaatccc tgtagtcaat gttgaaaacg aattgcttta catccttgaa
    10321 aactctgatg tgaaaatcca attctacgga actgaagtga tcggtgtcac cgttcctact
    10381 actgttgagt tgacagttgc tgaaactcaa ccatctatca aaggtgctac tgttacaggt
    10441 tctggtaaac cagcaacgat ggaaactgga cttgtcgtaa acgttccaga cttcatcgaa
    10501 gcaggacaaa aactcgttat caacactgca gaaggaactt acgtttctcg tgcctaatct
    10561 ctagaaagag gtcattctat gggaattgaa gaacaacttg gcgaaatcgt tatcgcccca
    10621 cgtgtacttg aaaaaatcat tgctatcgct actgcaaagg tagagggtgt tcactctttt
    10681 tcaaacagat cagtgtctga taccctttca aaactttcac tcggccgtgg catttatctt
    10741 aaaaacgtgg acgaagaact cacagcagat atctatctct accttgagta cggagtaaaa
    10801 gttcctaagg tagcggttgc tatccagaaa gctgtcaaag atgccgtccg taatatggct
    10861 gatgtagaac tcgctgctat caatattcac gttgcaggta tcgtcccaga taaaacacca
    10921 aaaccagaat tgaaagatct atttgacgag gacttcctca atgactagtc cactattaga
    10981 atctagacgc caactccgta aatgcgcttt tcaagctctc atgagccttg agttcggtac
    11041 ggatgtcgaa actgcttgtc gtttcgccta tactcatgat cgtgaagata cggatgtaca
    11101 acttccagcc tttttgatag acctcgtttc tggtgttcaa gctaaaaagg aagaactaga
    11161 taagcaaatc actcagcatt taaaagcagg ttggaccatt gaacgcttaa cgctcgtgga
    11221 gagaaacctc cttcgcttgg gagtctttga aatcacttca tttgacactc ctcagctggt
    11281 tgctgttaat gaagctatcg agcttgcaaa ggacttctcc gatcaaaaat ctgcccgttt
    11341 tatcaatgga ctgctcagcc agtttgtaac agaagaacaa taaaaagaaa aagtgtttga
    11401 tagatatcaa acactttttt ctttgcctcc cactatctaa aaaaatgata atagatataa
    11461 ttgtaaacaa aaatccagat aggttttgca tgattgagaa agttaaaaaa actatggcag
    11521 agaatcgtta atctcagatt gtcggtagaa cgataaacaa gggcaaaaaa gaaaccaatc
    11581 agactataat ataataaact aattggatct ctgtgagata gtatcaaatg gctaatccca
    11641 aagatgatag cagataggat aacatccaaa taatacttgg actagggaaa gaaggtgttc
    11701 ataaaatacc ctctatcaag agtctcctca aaaacaggac cgatgattac aggcaggaca
    11761 aaagataaga tagtcgataa aaaggttggt tgtccatttg aaagaaccac ggtaaaatac
    11821 tcatcatgaa tattcctatg attaatcaaa tgagcatagc gtgcccaaaa attaccgaga
    11881 atctgataaa ccacataagt tgcaaataag gagaagacaa atgaccagtc ccagctcttt
    11941 ttctttttta acctccaaat taatagaaaa ggaaacttcc cactaatccc actgttaaaa
    12001 taagagaata gacatcagct cctaacccta aaatgatcgt cacatacaat ccaattgttt
    12061 gtggtaaata ggtagatagt aaaataataa gcaaaaatat tccaaattgt cttagtttct
    12121 ttgtgtttct catcgtactt ttttgaaaga ttaccctgtt cggaagccgt acttccaagc
    12181 atctatataa gaattaagtg ccccttgcct catataggga gcaaattctc tataatataa
    12241 ccatctacta tatccatctt cccaaacagc aagaccactt gaagtttgct ccaagtcctc
    12301 agttgaaata actgtaaatg tttctgtacc tgtcattgca agtaccttct taaaatatag
    12361 tga
//
LOCUS       DAVHIU010000049        12352 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVHIU010000049 DAVHIU010000000
VERSION     DAVHIU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.051"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            2..1257
                     /locus_tag="VVN06_001190"
                     /pseudo
     CDS             2..1257
                     /locus_tag="VVN06_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1428..3752)
                     /gene="pflB"
                     /locus_tag="VVN06_001191"
     CDS             complement(1428..3752)
                     /gene="pflB"
                     /locus_tag="VVN06_001191"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU5562740.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            4100..5161
                     /gene="dinB"
                     /locus_tag="VVN06_001192"
     CDS             4100..5161
                     /gene="dinB"
                     /locus_tag="VVN06_001192"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5562741.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(5308..6153)
                     /locus_tag="VVN06_001193"
     CDS             complement(5308..6153)
                     /locus_tag="VVN06_001193"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5562742.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            6409..7277
                     /locus_tag="VVN06_001194"
                     /pseudo
     CDS             6409..7277
                     /locus_tag="VVN06_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7578..9467)
                     /locus_tag="VVN06_001195"
     CDS             complement(7578..9467)
                     /locus_tag="VVN06_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU5562743.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            9605..11170
                     /locus_tag="VVN06_001196"
     CDS             9605..11170
                     /locus_tag="VVN06_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU5562744.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYVSAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            11170..11910
                     /locus_tag="VVN06_001197"
     CDS             11170..11910
                     /locus_tag="VVN06_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5562745.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(12019..12234)
                     /locus_tag="VVN06_001198"
     CDS             complement(12019..12234)
                     /locus_tag="VVN06_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562746.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         3814 a         2657 c         2320 g         3561 t
ORIGIN      
        1 tatccagatt tcagacatca tcaataaaga tacacacaag gaaatcatcg ccaaactgga
       61 ctacgacgcc ccatcttgcc ctgagtgcgg aaaccaattg aagaaatatg actttcaaaa
      121 accgtctaag atcccttacc tcgaaacaac tggtatgcct tctagaattc tccttagaaa
      181 acgtcgtttt aagtgctatc attgcttgaa aatgatggtc gctgaaactt ctatcgtcaa
      241 gaaaaatcac caaattcctc gtattatcaa ccaaaagatt gcgcaaaagt taattgaaaa
      301 gatttctatg accgatattg cccatcagct ggccatttca acttcaactg tcattcgaaa
      361 gctcaatgat tttcacttta aacatgattt ttcttgtctt cctgagatta tgtcttggga
      421 tgagtatgct tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa
      481 tatcatcact gttcttgagg gtagaacaca agctatcatt cgaaatcact ttcttcgcta
      541 cgatagagtt gtccgatgtc gcgtcaaaat cattactatg gatatgttta gcccttacta
      601 tgacttggct aaacagcttc gctttcaaat ttctaggctc aggctgaaac agtctcccag
      661 actgtttcac tcccgaatgc aaaaattgtt cttgatcgct ttcacattgt acaacatctt
      721 agccgtgcta tgagtcgtgt gcgtgttcaa atcatgaatc agtttcatcg aaaatcccat
      781 gaatacaagg ctatcaagcg ctactggaaa ctcattcaac aggatagtcg taaactcagc
      841 gataaacatt tttatcgtcc tacttttcgt atgcacttaa ccaataaaga gattttaaac
      901 aagcttttga gctattcgga agacttgaaa caccactatc aactctatca actcttgctg
      961 tttcacttcc agaataagga accagagaaa tttttcgggc ttattgagga caatctaaag
     1021 caggttcatc ctatttttca gactgtcttt aaaaccttcc tcaaagataa agaaaagatt
     1081 gtcaacgccc ttcaactaca ctattctaat gccaaactgg aagcgaccaa taatctcatc
     1141 aaacttatca agcgcaatgc ctttggtttt cggaactttg aaaacttcaa aaaacggatt
     1201 tttatcgctt tgaacatcaa aaaagaaagg acgaaatttg tcctttctca agcttagctt
     1261 ttcttcaacc cactacagtt gacaaagagc ctgaaaactt caaaaaacgg attttcatcg
     1321 ctctgaacat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag cttttcttca
     1381 acccactaca gttgacaaag agcccttttt attattcaag aacttgatta gctcaatgca
     1441 tccaaggcgt catccattga aagaacttcg tggaagacac gttgtgtcaa ttcagttttt
     1501 tgttctggag tgaggtattt agtgtttaca cagtatccag agatacgtac gataacgtct
     1561 tcgcctgaca tgattttttc gtaaacatcg ttcaagtcca taacgttcaa gttaacgtgt
     1621 tgtccaccgt tttcgaagta accatcaagg attgttacca agttatcaac ttgttcatca
     1681 cgagtcttac caagagcgcg aggtgatact tgtgtagtca atgagatacc gtcagctgca
     1741 taactaaagt caaggctaga aagtgagttc aagttttgca accaaccacc tttagctttg
     1801 ttagatgggt tagcacctgg tgagaagaat tcaagtttag acaagttcac agaaccatct
     1861 tcgttgaggt atacaccttt gtgaactggt gagttaccag tttgtttaga gtaagcaacg
     1921 ttagatgtga ttgtcaaaag tgatactgta gcttctgcgt ctttgtatag tttgtggcta
     1981 cgtagacgag ttgtgtaagc ttcgatcaac cattctgcca attcgtttga acgtgggtca
     2041 tcttcacccc agcgtgggta gtcaccgatt gtttcgtaat cgtagatgta gccatcttca
     2101 tcacggattg gtttaactgt agcgtattta atcgctgaca atgtatcaac agtgttagca
     2161 aatccacaaa taccgaatcc catgttggca cgttgtttag ttggcaagaa ggccatttga
     2221 acagcttcgt agttgtactt atcagtcatg tagtggatga tgttcaaggc atctacgtaa
     2281 gtgtcagtca accagtcaag agatttttca aagttcgctt taactgattc aaattcaaga
     2341 acttcgtcac gaataggatc gatgtcaaat actttgtagt ctttgtgaac atcgtcgtaa
     2401 ccaccattca aaccagtaag aagggcttta agaacgttta cacgagcacc gaagtactgg
     2461 atgttgtggc gttgttcttc attttctgga tcaagtggag acacacagca tgagatacag
     2521 ctcatttcac catatccgtc tttagccatt gttgttacac cttcgtattg gatagaagag
     2581 tgtttgtggc tcatgtgcat acagtagcga cggaagttgt atggcaattt gtcagtccaa
     2641 agaactgtca agtttggttc tggtgagtta ccgatgttgt caagagtgtt caagaaacgg
     2701 tagtccatct tagtaacacg gtgacgaccg tcgttaccca taccagccat agaagttgtg
     2761 ataaaggttg ggtcacctga gtacaattgg tcataagctt tggtacgagc aaatttaact
     2821 gtacgaagtt tcataacgaa atcatcaacg aattcttgga tttctgattc agtaaatgta
     2881 ccacgagcaa ggtcacgttc tgcaaagatg tccaatacga ttggtacacg acctagagat
     2941 gtagcagcac cgttaatcac acggcagaca gccatgaaag caatgttaac ccattggatt
     3001 gcttctttca cgttcatcgc tggtttgcga acatcaaccc cgtaaaggtc acccaggcga
     3061 acaacttgtt gcaatgcttg gtattgaagg tttacttctt cacgaagacg gattgtttct
     3121 tcatcgattt ctttgattgc attccagtcg tttacttttt cttgcatcaa gtagtctgca
     3181 ccgtaaagag caagacgtgc gtaaacaccg atgatacgtc cgcgtgagta tgcatctgga
     3241 agaccagtta cagtgtgtgc atgacgagcg cgacgaatat ttgaagtgta ggcacggaaa
     3301 ataccgtcgt taactgttgt tacatattta gtgaagattt cgtgaacagc tgggtctggt
     3361 tcgtatccat tttcttttaa agtagtttca gccatacgga taccaccttt tggcatgaag
     3421 ttcaatttga agagttcatc attttggata ccaaagataa cttcattttc tttgtcgata
     3481 aatccagcag ggatatcagc gatagatgtt ggacgagtgt ccattgggaa acgagtttct
     3541 tcgtagtgtg ctttagtttc ttctacaatt ttcttgatgt gaagtgaacg ctctgttggt
     3601 cctgcaagga agctttcgtc tccatcataa ggtgtgtagt tagcttgtac aaagcgtgat
     3661 acacttgctt tttctttcca atctacgcct ttgaagcctt cccaagcttt gtcaaaaata
     3721 tcttgtgctt caacaactgt cttaacaacc atattaatgt cctctttttt ctagtaacag
     3781 tcatctgtta cattcatgag acaagtatac catatagtaa ccgatttcaa caagtgaaaa
     3841 atccctattt ttacactttc ttttctaaaa cagtctatat tttatttcaa actgtattat
     3901 atttttgaaa aaataaagtc cttttttctt tttttcagaa aaaagggtat aataaaagaa
     3961 aataagcagt aacactcaat ggaaatcgaa aagcaaacta ggaagctagc cgcagatttc
     4021 tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga ttttcgaaga
     4081 gtataaaaag gtgctaggca tgttgatttt tcctttgtta aatgatttgt caagaaaaat
     4141 catccatatt gacatggatg ccttttttgc tgcggtggaa atcaaggata atcctaaact
     4201 cagaggaaaa cctgtcatta ttggaagcga ccctcggcaa acaggtggac ggggagtcgt
     4261 ttctacctgt agctatgagg ctcgagcttt tggtgtccat tctgccatga gttccaagga
     4321 agcttatgaa cgttgtcccc aggccgtctt tatctcagga aattatgaga aatacaaagc
     4381 tgtgggactc cagattcgag ctatttttaa gcgctataca gatttgattg aacccatgag
     4441 cattgacgaa gcctatttgg atgtgacaga aaataaactc ggtatcaagt cagcggtcaa
     4501 aattgctcgc ctcattcaaa aagatatctg gcaagaactc catctaactg cttctgccgg
     4561 tatttcctac aacaagttct tagctaaaat ggcgagtgat tatcaaaaac ctcatggttt
     4621 gacagtgatt ctacctgaac aggctgagaa ttttctcaaa caaatggata tttccaaatt
     4681 tcatggagta ggaaaaaaga cagtagaacg tcttcatcaa atgggcgttt ttactggtgc
     4741 tgatttactt gaagttcctg aggtgaccct aatagaccgt tttggtagac taggctatga
     4801 tctgtatcga aaggctcgtg gcattcacaa ctctccagtc aaatccaatc gcatccgtaa
     4861 atcaatcggc aaggagaaaa cctacgggaa gattctccgt gctgaggaag atatcaaaaa
     4921 agagctgact attctatcag aaaaagtcgc tctcaatcta catcaacaag aaaaagctgg
     4981 aaaaattgtc attttgaaaa tccgctacga ggacttttca actcttacca aacgaaaaag
     5041 tcttgctcaa aaaacacaag atgctagtca gataagccaa atagccctgc aactctatga
     5101 agaattaagc gagaaagaaa gaggtgtccg cctattgggg attaccctga ctggatttta
     5161 aagctcaatg aaaatcaaag agcaaactag gaagctagtc gcaggctgct caaaacactg
     5221 ttttgaggtt gcagataaag ctgacgcggt ttgaagaaat tttctaagag tataaaacct
     5281 tgaagagttg ccccttcaag gtttttctta tacaaataaa cggacaaaac tgtaaagtag
     5341 caaaacacta ccaagcacga tacggtattt accaaaaagg gtgaagtcgt gttttttcac
     5401 atagctggtc aagaagcgaa tagccaccat gctgaccgca aaagctactc ccatcgcgac
     5461 caagagcaaa aacaattgcc caaagctcaa gagttctccg gctttcacaa atttgaaaat
     5521 ctttaaggca ctagctccaa acataacggg aatcccaaga tagaaggtaa attctgtcac
     5581 aactgaacga ctggttccat ttaacaaacc accgacaatc gttgcacctg aacggctagt
     5641 ccctggtaaa agagcaagaa cttggaagag tccgatatag aaagcggtcg tataaggaag
     5701 cttgtccaac tctgttacac ttggctcgat agcacgcgct ttattgcgct tttccaaata
     5761 gatgaaggca accccgtaga taatcaacat gagagcaact gaaaccatgt tatggaagtg
     5821 ggtatcaaac caatcatcaa atttaaagac accaagtaaa ggcaaagtgg caatcaagac
     5881 cttcaaccat agtctccaag tcttacgaac ttcctgtttg tccttagtcg gtttaaaagg
     5941 attgagcttg ttaaaataaa tcaccataac tgctaaaata gcaccaagct gaatcacgac
     6001 attaaacatg gacataaagg cttcattttg attttggtat tggataaact cctctgctaa
     6061 aatcaagtga cctgtactgg aaatcggcaa ccattccgta attccttcaa caatcccgaa
     6121 gaagatagat tttaaaattt caataagata catatatttc tccttatatt atatttaacg
     6181 cagttagcta ctgccaaagc ccaagtggta tacttggaat aagccactgt ggattagttc
     6241 atttcctcca atcgcttgac gatttccgaa agggtctaac cacagctttt tgtttcaatt
     6301 ggtaatgagt ttgataacgc atagacgttt tatctcgtct aaggtaacaa ggcaaaaagt
     6361 tcgtggataa ccaagtagac ttgaaatact atatctggag gtactcccat gctttatgtg
     6421 ggcattgatg tcgctaaaaa taaacacgat gttacagcct tgaatgttcg aggaaaactg
     6481 ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta gatctgtctt
     6541 ttcgacagct caaccaagac tgtctcatcg ctcttaaact tctttctgac cccaatcgtg
     6601 aacaatttca acacgataat cggcaagtag aactaaaaat actggctaga catattcatc
     6661 gtctcaagaa aaaacagtct gattggaaag tacaatacac tcgttgtctt gatatcatct
     6721 ttcctgagtt ggataaaatc gttggaaagc attcagaata tacctaccaa ctcttgacac
     6781 gctaccctaa tcctcagaaa aggcttgagg caggatttga taagctgata gaaattaagc
     6841 gattgaccgc ttctaaaatt caggatatcc tctcagtcgc acctcgttct atcggaacaa
     6901 catctcctgc tcgtgaattc gaaatcatcg aaatcatcaa acattacaag aggctcattg
     6961 acaaggcgga aacatgtgtc aatgacttga tggctgagtt caactcggtc atcacgacgg
     7021 ttactgggat tgggggtcgt ttaggggcgg tcattttagc cgagattcga aatattcatg
     7081 cctttgataa tcctgctcaa ttacaagctt tcgctggact ggattcttct atttatcagt
     7141 caggtcagat tgatttagct ggaagaatga tcaaacgggg ttcccctcat ctgcggtggg
     7201 cactcataca agctgccaaa gcatgcgctc gcttttcacc tgcttttaag gcctatctta
     7261 agactaagtt agaataagga aaacattaca atgtagccat catccacctt gcaaaaaaac
     7321 ttatccgaac cctgttttat atcctaaaaa agagctgcca tttgacgaac aaaaagtgat
     7381 gtaatttctg aaattaacac aggattacct ttgaaaaaat ggactttttc aagggctttt
     7441 tgccgtgctc taaaatcatc gccaaatcat caaattgaaa taagaaatag tttagataaa
     7501 actattgact tttcttatag tttgtctttc ttctatcttc cattatatca tactttttca
     7561 gtctatggta gctctcttta aaaggctccg atactgccgc cacctccgcc tccagagaag
     7621 ccaccaccag aacttccact tccagaagat acagagtagg tacttgctgt atttgcgaca
     7681 ctagcataat ggctcatttg cgcgcttgaa tgataaaaca tactgtgcca gccataagct
     7741 acatagagat tgatatctgg attttcaact tgaatctgat gaaccttcat caaatgacta
     7801 accttgtccg catagccaaa taaggttgca tagaccaaga gacgattcca aacaacaata
     7861 ctttccaatt cagccttatc cagtcgtgca atctcacgta acatattttc aaaactggtc
     7921 cagagatagt agacctcagc tcccgcttca tttagaacac catcacgatt atctagtcga
     7981 agcttccaat aatagaaaac agacaaaact aaccctagaa aaccaagtat tggcaaaggg
     8041 aggtaaagat agccatgaac gtctaaactg tacaagaaca aaccaaatcc gataaatagg
     8101 ggcaagatag tcaagacacc catacccact tgcaaagcct tttccaaacc agttaaagga
     8161 cgatagtaat ctgggagtcc ccagaagaca actcgctttc tcactccttc ttgcatctct
     8221 tttaatactg cttcgaaaga agatttgagc tgaatccctc ttgcttgaat ccgtttctca
     8281 tcagcagctt tagctccgcg ataaaggctg tcagatacct tgtaatccgc aaacaaattg
     8341 gaaagagtgg cttctttttt gcctgaaaag gctagattta gacagtctct ctcaaatctt
     8401 gacaaaccat cttctttcac caatctcaag ccaactgcat ctccttctga aataatagaa
     8461 acattcccac ggtccatcac atctagcaag gtagcttgaa taagttggtc aaaagtgaat
     8521 ttgccagccc ccttgactaa gggactcact tcctccaagg aggtcgagta gacagcctct
     8581 gataaaacca taggctctaa ttccattggt ggttcataga gacgatgatt tttggcatat
     8641 ttgactgaag gagtggtctt tcttctataa ataaaataga agcagacact caataacaag
     8701 gagatagaaa ggatcgaagg gaggacccaa gtaacaagtt gtttactctg atctttttct
     8761 ctaacaatcg agtcttctat cttattaaac tcttctaaac gattcccttt caatccctga
     8821 tccctagcgc tagcaaaatc ggtccgaggc cagtaggcat gcaactcaac tccacgctta
     8881 gccggaagat tgtctaaacg gatagtataa tcaaggttac tcttttcaat cgttccctct
     8941 ctaaaaagtt tccctgtatg gaaaaagagt ttttcagccc ccttgtctcc ccttacatga
     9001 aattcaaact tttcaataga ctctgaacta tctgtcagag gttgccaatt taattcagcg
     9061 atatcatcat aaaggaaaag taaatttttt aagttccaga cgaggtcaac ttcaactatg
     9121 tcgccctcct gacctggatt atagactctc acagtataac catccgcttc ttctgttact
     9181 tcgctagtca catctgctag ttctgcaccg tttttcgcgg cctgaacctt tggatgaggg
     9241 tcaatgtcaa acccgctagg catcttacca gcacgtccaa gtcccacgat ttgtccctta
     9301 aagtcctcct caaactggta aactatcttc tgtctaaact ctgccgtatt atctgcatga
     9361 atatacaaat ccccttgata agagtttatc ttgaaatcaa tggcaaaaac agagagtgga
     9421 agaaggcaaa acaagcctaa caccagtaag aaaaaagttt ttttcatcaa ctaagccccc
     9481 tttttatctt tcttatcatt atagcatttt ttcgtaagta aaggcttact tatgttgaaa
     9541 aattacgcat ttttcgataa aataggagac agttattttt taatagatca gaataggaga
     9601 aatcatgaga aaaatatact tatctatttt cacaagtctc ttgctgatgc taggacttgt
     9661 caatgttgct caagccgatg aatatttacg catcgggatg gaagcagcat atgctccctt
     9721 taactggacc caggatgatg atagcaacgg agctgtcaaa atcgatggga ccaatcagta
     9781 tgccaacgga tacgatgttc aaatcgccaa gaaaatcgct aaggacttag gtaaagaacc
     9841 tttggttgtt aaaaccaagt gggaaggtct agtccctgcc cttacttctg gtaagattga
     9901 catgattatc gcaggtatga gtccaactgc agaacgcaaa caagaaattg ccttttcgag
     9961 cagttactac actagcgaac cagttttgct tgtcaaaaaa gattctgcct acgtaagtgc
    10021 taaatctttg gatgacttta acggtgcaaa aatcacttct caacaagggg tctacctcta
    10081 cgacttgatt gcacaaatcc caggtgctaa aaaagaaaca gccatgggag acttcgctca
    10141 aatgcgccaa gctcttgagg ctggtgtcat tgatgcttat gtttctgaac gtccagaagc
    10201 actgactgct gaagctgcga actctaagtt caagatgatt caagtagaac ctggtttcaa
    10261 aactggggaa gaagatacag ctatcgctat cgggcttcgt aaaaatgaca atcgtattag
    10321 ccaaatcaat gccagcattg aaaccatttc aaaagatgac caagttgcct tgatggatcg
    10381 tatgatcaag gaacaacctg ccgaagctac aacaactgaa gagactagca gtagtttctt
    10441 tagccaagtc gctaaaattc tttctgaaaa ctggcaacaa ctcttgcgtg gtgctggtat
    10501 cactctttta atctctatcg tcggaaccat cataggtctc attattggac ttgccattgg
    10561 tgtcttccgt actgctcctc tctctgaaaa caaagccatt tatggccttc aaaaactagt
    10621 cggctgggtt ctcaatgtct acattgaaat tttccgtggt acgccaatga ttgttcaatc
    10681 gatggttatc tactatggaa ctgcccaagc tttcgggatc aaccttgacc gtacactagc
    10741 tgctatcttc atcgtttcaa tcaataccgg tgcctacatg actgaaatcg tccgtggtgg
    10801 tatcctagca gttgacaagg gacaatttga agctgcgact gctcttggta tgacccataa
    10861 ccagaccatg cgtaagattg tcctacctca ggtagtccgt aacatcctac ctgcaactgg
    10921 taatgaattt gtcatcaata tcaaagatac atctgtattg aacgttatct ctgttgtcga
    10981 actttatttc tcaggaaata ccgtggcaac acaaacctat caatacttcc agacatttac
    11041 aatcatcgcc gtgatttact ttgtcctcac cttcaccgta acacgtatcc tacgctttat
    11101 cgagcgcaga atggacatgg atacctacac tacaggtgct aaccaaatgc aaacggagga
    11161 tttgaaataa tgacacaagc aatccttgaa attaaacacc tcaaaaaatc ctatggacaa
    11221 aacgaagtgc taaaagacat ttcactcact gtccacaagg gagaggtcat ctctatcatc
    11281 ggaagctctg gaagcggaaa atcgaccttc ctacgctcca ttaacctact tgaaacacca
    11341 actgatggac aaatccttta tcatggacaa aacgtcctcg aaaaaggcta tgacctcacg
    11401 caataccgtg aaaagttggg gatggttttc caatccttta acctctttga aaatctcaat
    11461 gttcttgaaa acacaatcgt cgctcagaca actgtcctaa aacgcgaacg cacagaagct
    11521 gaaaagattg ccaaagaaaa cctggaaaag gtcggcatgg gagaacgcta ctggcaagcc
    11581 aaaccaaaac aactctcagg tggtcaaaaa caacgtgtgg ccatcgctcg tgccctctcc
    11641 atgaatccgg acgccattct ctttgatgaa ccaacatcag ctctcgatcc agaaatggtt
    11701 ggagaagtcc tcaaaatcat gcaggacctg gctcaggaag gcttgaccat gattgtcgta
    11761 acccatgaaa tggaatttgc ccgtgatgtc tctcaccgtg ttatctttat ggataagggc
    11821 gtgatcgctg aagaaggtaa accagaagac ctcttcacca atcctaaaga agaccgaaca
    11881 aaagagttcc ttcaacgcta tctcaaataa aaagaaaagg ctgcatcaac cgtgcagtct
    11941 ttttgctgtc ctcaaaatga aaaggcagat tctctatctc tactacttga aggtcaagaa
    12001 tccgccgtta tccaaagatt aatcttcaaa tttttcataa tttttttcat agaaatcaat
    12061 taggcctagg gctgtttcaa atgaaatatt ttttactttt gcacgaccct gcgccagagc
    12121 aatgatagac atttcacgcg cattcgtttc tttagagata cggtagcctg tgattttctt
    12181 atcacgaacc cagccaacaa ctgattctac tttttcaaag tttgacttag ccatgttttt
    12241 ctcccatttt cttctttata atatttaatt gtatacgttt ttactacttt tgtcaataaa
    12301 aaaaacatat attcaacgaa aataattagt tcagaatttt cttacttcct tt
//
LOCUS       DAVHIU010000050        12134 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVHIU010000050 DAVHIU010000000
VERSION     DAVHIU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.052"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            93..1118
                     /gene="trpS"
                     /locus_tag="VVN06_001199"
     CDS             93..1118
                     /gene="trpS"
                     /locus_tag="VVN06_001199"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5562747.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1270..2748
                     /gene="guaB"
                     /locus_tag="VVN06_001200"
     CDS             1270..2748
                     /gene="guaB"
                     /locus_tag="VVN06_001200"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5562748.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2799..3896)
                     /gene="recF"
                     /locus_tag="VVN06_001201"
     CDS             complement(2799..3896)
                     /gene="recF"
                     /locus_tag="VVN06_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU5562749.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3899..4267)
                     /gene="yaaA"
                     /locus_tag="VVN06_001202"
     CDS             complement(3899..4267)
                     /gene="yaaA"
                     /locus_tag="VVN06_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU5562750.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4426..5676
                     /locus_tag="VVN06_001203"
     CDS             4426..5676
                     /locus_tag="VVN06_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5562751.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLALDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILDETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERTYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5673..6956
                     /locus_tag="VVN06_001204"
     CDS             5673..6956
                     /locus_tag="VVN06_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5562752.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            6998..7819
                     /locus_tag="VVN06_001205"
     CDS             6998..7819
                     /locus_tag="VVN06_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562753.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7830..8375
                     /gene="pgsA"
                     /locus_tag="VVN06_001206"
     CDS             7830..8375
                     /gene="pgsA"
                     /locus_tag="VVN06_001206"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5562754.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIVLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8372..9199
                     /locus_tag="VVN06_001207"
     CDS             8372..9199
                     /locus_tag="VVN06_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU5562755.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9184..10023
                     /locus_tag="VVN06_001208"
     CDS             9184..10023
                     /locus_tag="VVN06_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU5562756.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10016..10810
                     /locus_tag="VVN06_001209"
     CDS             10016..10810
                     /locus_tag="VVN06_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5562757.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10869..11687
                     /gene="mreC"
                     /locus_tag="VVN06_001210"
     CDS             10869..11687
                     /gene="mreC"
                     /locus_tag="VVN06_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5562758.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11687..>12134
                     /gene="mreD"
                     /locus_tag="VVN06_001211"
     CDS             11687..>12134
                     /gene="mreD"
                     /locus_tag="VVN06_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5562759.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNF"
BASE COUNT         3690 a         1831 c         2602 g         4011 t
ORIGIN      
        1 tgtaaaaagc aaggctgatt tattttgata aattacggtt atttcattaa aaaaatgcta
       61 taattgaaag gactatatcg aaggagaaca aaatgactaa acccattatt ttaacaggag
      121 accgtccaac aggaaaattg catattggac attatgttgg aagtctcaaa aatcgagtat
      181 tattacagga agaggataag tatgatatgt ttgtgttctt ggctgaccaa caagctttga
      241 cagatcatgc caaagatcct caaaccattg tagagtctat cggaaatgtg gctttggatt
      301 atcttgcagt tggattggat ccaaataagt caactatttt tattcaaagc cagattccag
      361 agttggctga gttgtctatg tattatatga atctagtttc gttagcacgt ttggagcgaa
      421 atccaacagt caagacagag atttctcaga aaggatttgg agaaagcatt ccgacaggat
      481 tcttggtcta tccaatcgct caagcagctg atatcacagc tttcaaggct aattatgttc
      541 ctgttgggac agatcagaaa ccaatgattg agcaaactcg tgaaattgtt cgttctttta
      601 acaatgcata taactgtgat gtcttggtag agccggaagg tatttatcca gaaaatgaga
      661 gagcagggcg tttgcctggt ttagatggaa atgctaaaat gtctaaatca ctaaataatg
      721 gtatttattt agctgatgat gcggatactt tgcgtaaaaa agtaatgagt atgtatacag
      781 atccagatca tatccgcgtt gaggatccag gtaagattga gggaaatatg gttttccatt
      841 atctagatgt ttttggtcgt ccagaagatg ctcaagaaat tgctgatatg aaagaacgtt
      901 atcaacgagg tggtcttggt gatgtgaaga ccaagcgtta tctacttgaa atattagaac
      961 gtgaactggg tcctattcgt gagcgccgta ttgaatttgc taaggatatg ggagaagttt
     1021 ataatatgat tcaaaaaggt agtgaaagag cgcgtgaagt tgcgggtcaa accctatctg
     1081 aggtaaaagg ggcaatggga cttcattact ttaactaagt ttattttaca agaaactatc
     1141 atttctatct cgaagaaaag atagtttttt atttacccct gtaacatttg acaaattttt
     1201 atagaatggt atgatagata caatataaaa agagtcaagc tcaaaaagaa agaaaagagg
     1261 aaacttcgaa tgtctaattg ggacactaaa tttttgaaaa aaggttttac ctttgatgat
     1321 gtattgctta ttccagctga aagtcatgtg ttgcctaacg atgcagattt aacaactaaa
     1381 ttggcagata atttgacttt aaatatccca attattaccg ctgccatgga cacagttaca
     1441 gagagtcaaa tggccattgc tattgctcgt gcaggtggtc tcggagttat ccataaaaac
     1501 atgtcaattg ctcaacaagc agacgaggtt cgtaaggtaa aacgttctga aaatggagtt
     1561 attattgatc cgttcttctt gacgcctgaa catacaattg ctgaagcaga tgagcttatg
     1621 ggtcgttacc gcatcagtgg tgttccagtt gttgaaacac ttgaaaatcg taaattggtt
     1681 ggtattttga caaaccgaga tcttcgtttt atttcagatt ataatcaacc aatttcaaac
     1741 catatgacta gtgaaaatct tgttactgct cctgtgggta cggatcttgc aacggctgag
     1801 agtattcttc aagagcatcg tattgaaaaa cttccgttgg tcgatgaaga aggcagtctt
     1861 tctggtttga tcactatcaa agatattgaa aaagttattg agtttccaaa tgcggctaaa
     1921 gatgaatttg gtcgtcttct agttgcaggt gcagtaggtg ttacttcaga tacatttgaa
     1981 cgtgcagagg ctctttttga ggcaggagcg gatgcgattg ttattgatac tgcacatggt
     2041 cattctgcag gtgtcttgcg taaaattgcc gagattcgtg ctcatttccc agatcggact
     2101 ttgattgctg gaaatattgc tactgctgaa ggtgcacgtg ccctttatga agcgggtgta
     2161 gacgttgtta aggttggtat tggaccaggt tctatctgta ctactcgtgt gattgctggt
     2221 gttggtgttc cgcaagtaac agctatctac gatgctgcag ctgttgcgcg cgaatatggt
     2281 aaaacgatta ttgctgacgg tgggatcaag tattctggag atattgtaaa agcacttgct
     2341 gcaggtggaa atgctgttat gcttggatct atgtttgctg gaactgatga agctccaggc
     2401 gaaactgaaa tcttccaagg acgtaaattc aagacttacc gtggtatggg atcaattgct
     2461 gctatgaaga aaggttcaag cgaccgttat ttccaaggtt ctgtcaatga agcaaacaag
     2521 ctcgttccag aaggaattga aggtcgtgtt gcttataaag gagcggcagc tgatattgtt
     2581 ttccaaatga ttggtggtat tcgctctggt atgggttact gtggtgcagc taaccttaaa
     2641 gaactacacg ataatgctca atttattgaa atgtctggtg ctggtttgaa agaaagccat
     2701 cctcatgatg tgcaaattac taatgaggca ccaaattatt ctatgtaaaa aacaatgaaa
     2761 agaactccag tgaaaacagg agttctttta caatgttgtc aatttccatt tacagaagct
     2821 ttaccatcct gaatagtgaa gatacttaga ttttctggca gattttgaag atggtctaag
     2881 cttgttgttg tgataaaggt ttggattgat tgagaaatcg tttctaataa ttttaactgt
     2941 ctagtgttgt caagttcact catcacatcg tcaagcagta atataggaga ttctgtggta
     3001 atgctttcca ttaattcgat ttctgctaat tttatcgaga ggacgagact acgatgttga
     3061 ccttggcttc cgaaactagc atccatccca tttatataaa aagaaatgtc atctcgatga
     3121 ggaccgacac cagtattctt tttaaataaa tctctggatc tacttttttc taaagcaatt
     3181 ttgaaagatt cggataagtt ttgtttgtca gttatattga cagaagattg ataggatatt
     3241 gacaactctt cgatctgatt agagagttca aaatgtttct tacgcccaaa tgattctagt
     3301 ttttttatga aatctaagcg gtgattcatt acacgacatc cataatcaac tagctgatca
     3361 tctaacacag aaaggaatgt ttcatctatt ttttgagctg attttaggta agtgtttctt
     3421 tgctttagga tgtggttata attggttaag tcagataaat agattggctt aatttgccca
     3481 agttccatat caatgaattt tcgtcgaatc gaaggtgctc ctttaattag ttgtaaatct
     3541 tcaggagcaa ataagacaac attcatgtgt cctacataat ctgaaaggcg tgcctgtttt
     3601 aagtgattaa cttttgttac acggcctttt tgtgttagtt cgatttctag gggaatggat
     3661 ccagtttttt tctgaacgag acctgaaaga tgaagttgtt cctcatcaaa atgaatgaga
     3721 tttttatctg ttcgagttcg atgactacgc gttaaggcta aaaaatagat agcctctaac
     3781 atgtttgttt ttccttgtgc attacgtccc aaaaagacat tcaatttagg attaaagtct
     3841 attttcgttt ctttgtaatt acgaaaagtc ttgagagata gatgttgtag ccacatgatt
     3901 atctaccagg gaatcgtgga gcttgttggc ttttaggtga tgaagcaggt ttcgatttgt
     3961 cttttttaac tcccttattc atctttttga caagtttagc gatccgttct ttttcaactt
     4021 tatcagcttg gtattcatct tgttcttcag aagtaggttg tgtcaacaag atgtcaatgt
     4081 tcatgtcagg gatgtcaact ttatcgccaa tacgaagttt ttttccacga cgactctcta
     4141 attccctatt aaagtaaaca gaatgttcag agagaaatga tttaatagct cctccgctat
     4201 gtgtaattcc aagttctttg agtagtgctt ggagggtaat aaattcttca aataatttgt
     4261 attccataat tcacctcaat ctttggtatt ataccatatt ttcctaaaaa tagtgagaga
     4321 gacagcgaga aatgtaagcg atttttattt caaaagtgtt acagggaaca agaaaatttc
     4381 aggttttcgt gatataatag aagtctgtat ataaggaggt aaatcatgga gttagtgcat
     4441 ggaatttcaa cacattttat ccaatcaaaa aagtttaaaa caaacaaaat taccgtgcgt
     4501 tttaccgctc cattagccct tgatacgatt gcaggtcaca tgttgagtgc aagtatgcta
     4561 gagactgcta atcagatgta ccccacttct caagatttga ggagacactt ggccagtcta
     4621 tacggtacag atatgtcaac caattgtttc agaagagggc aaagccacat tatagaattg
     4681 acatttacct atgttcgtga tgagttttta agtaggaaaa acgtgctaac ctctcagatt
     4741 ttggaacttg taaaagaaac tcttttttca cccgtagtag ttgataatgg gtttgatccg
     4801 gccttatttg aaattgagaa aaaacaattg ctagcaagtt tagcagctga tatggatgat
     4861 tctttttatt ttgcacataa agaattggat aaattgtttt ttcatgatga acgtcttcaa
     4921 ttggaatata gtgatttacg aaatcgtatt ttagatgaaa ctccacaaag ttcttattct
     4981 tgtttccaag aatttttagc caatgatcga atagatttct ttttcctagg tgattttaat
     5041 gaggttgaaa ttcaaaatgt attagaatca tttggcttta aaggtcgaaa aggagatgtg
     5101 aaggttcagt attgtcaacc ttattctaat atccttcagg aaggtatggt tcggaaaaat
     5161 gtgggacaat ctattttgga attaggttat cattaccgtt ctaaatatgg tgatgagcaa
     5221 catttaccca tgattgtaat gaatggttta cttggtggat ttgctcactc taagctcttt
     5281 acaaatgtcc gtgaaaatgc tggattagct tataccattt caagtgagct tgatttattt
     5341 agtggattct tgaggatgta tgctggtatc aatcgagaaa atcgtaacca ggctcgtaaa
     5401 atgatgaata atcaactgct tgatttaaaa aaaggttatt ttacagagtt tgagttaaat
     5461 cagaccaagg aaatgattcg ttggtcgttg ttactttctc aagataatca atcttcattg
     5521 attgaacgta cttatcaaaa tgccttattt ggaaaatctt cagcagactt taaaagttgg
     5581 attgcaaagc ttgaacaaat tgacaaagat gctatttgta gagtagctaa taatgtgaaa
     5641 ctacaagcga tttactttat ggaaggaata gaatgacaaa ggttgttttt gaagaaaaat
     5701 actatccagc tgtaaaagaa aaggtttatc gaactcgttt ggccaacgga ttgacagttg
     5761 ctcttttgcc taaaaaggaa tttaaagagg tttacgggag tgtcactgta cagtttggtt
     5821 cggtagatac gtttgtcaca gaagttgacg gagatgtaaa acaatatcct ggaggaattg
     5881 ctcattttct tgaacataaa ttatttgaga gagaagattc tagtgatttg atgtcggctt
     5941 ttacgagtct aggtgcagat agtaatgcct ttacaagctt tacaaaaaca aactatcttt
     6001 tttcagcaac ggattatttt ttagaaaatt tatatttact tgatgaattg gtaacatcag
     6061 cacactttac tgaagcttcc attctgacag agcaggatat tattcagcaa gaacgagaaa
     6121 tgtaccaaga tgatccagat tcgtgtttat tcttttcaac tttagcgaat ttgtatcctg
     6181 gtacaccttt agcaactgat atagttggaa gtgaggagtc catttcccaa atcaatctaa
     6241 ctaatttgca agaaaatttt acaaagtttt acaaacctgt aaacatgtct ctgtttttag
     6301 ttggtaattt tgatgtggag cgagtacagg actattttga aagcaaagaa ctgaaagatt
     6361 cagattttca ggaagtagca agagaaaagt tgtttttaca gcctgtaaag ccaacagata
     6421 gtatgagaat ggaagtatct tctcccaaac tagcgattgg agttagaggt aagcgagaag
     6481 tttctgaagc ggattgctat cgacatcata ttttattaaa attattgttt gcaatgatgt
     6541 ttggttggac ttcggatcgt tttcaaaaat gttatgaatc aggtaaaatt gatgcatcct
     6601 tatctctgga agttgaagta acaagtcgct ttcattttgt catgttgaca atggatacga
     6661 aagagccagt tgctttgtct catcaattta ggaaggctat tcgtaatttt acaaaggatt
     6721 tagatattac agaggaacat ttagatatta tcaaaagaga gatgtttggc gaatttttca
     6781 gtagcatgaa ctctcttgaa tttattgcaa cgcaatatga tgcttttgaa aatggtgaga
     6841 caatttttga tttgccgaaa attttacagg aaattacttt agaggatgtc cttgatgctg
     6901 gacatcattt aatagatgat ggtgacatag ttgattttac aatattccca tcgtagtaac
     6961 ctattataat agacactaga aagaagggat gacaagtatg agaaaaaaaa caattggaga
     7021 ggttttacga ttagctagaa tcaatcaggg attgagttta gatgaattgc agaaaaagac
     7081 agaaatccag ttagatatgt tggaagcaat ggaagcagac gatttcgatc aacttccaag
     7141 tcctttttac acgcgttctt tcttgaaaaa atatgcatgg gctgttgagt tagatgacca
     7201 aattgttttg gatgcttatg attctgggag tatgattact tatgaggaag tagatgttga
     7261 tgaagatgag ttgacaggtc gtagacgttc aagtaagaaa aagaagaaaa aaacatcatt
     7321 tttaccttta ttttatttta tcctttttgc tttatcgatt ttaatttttg tgacttatta
     7381 tgtttggaac tatattcaaa ctcaaccaga ggagccttct ctttctaatt acagtgtggt
     7441 tcaatcaaca agttcaacta gctctgttcc ccactcctca agtagtagtt cttctagtat
     7501 agaatcagct ataagtgtat caggcgaagg aaatcatgta gaaatcgctt ataagacaag
     7561 taaggaaaca gttaaattgc aattggcagt ttcagatgtt acaagttggg tcagtgtttc
     7621 agaaagcgaa cttgagggcg gtgtaacctt atcgccaaag aagaaaagtg cagaagcaac
     7681 agttgcaact aaaagtcctg taacaattac gttaggtgtt gtaaaaggtg ttgatttgac
     7741 agtagataat cagactgttg atttatcgaa attaacagct cagactggac aaattactgt
     7801 aacctttact aaaaattaag gaaaaacgaa tgaaaaaaga acaaattccc aatctcttaa
     7861 caataggtcg aattctcttt atacctattt ttatctttat tttaacgata ggaaattcga
     7921 tagagagtca tatagttgca gctattatct ttgctgttgc cagtattacc gactatttag
     7981 atggatattt agctcgtaaa tggaatgtgg tcagtaattt tggtaaattt gcagatccta
     8041 tggcggataa gttactagtt atgtcggctt ttattgtgtt gattgagtta ggtatggctc
     8101 cggcttggat tgttgcagtg attatctgtc gtgagttagc tgtgacaggt ttaaggcttt
     8161 tattggttga aactggtgga acaattttag cagcagcaat gcctggaaaa attaaaactt
     8221 ttagtcagat gtttgccatt attttcttgc tattacattg gactttgctt ggtcaagttc
     8281 tactttatgt agccttattt ttcactatct actctggcta tgactatttc aagggtagtg
     8341 cctatgtatt taaagggaca tttggttcga aatgaaatca ataattgatg taaaaaatct
     8401 ttcttttcgc tataaagaaa atcagaacta ctacgatgtg aaggatatta cgtttcacgt
     8461 gaaacgtgga gaatggcttt cgattgtagg gcataatggt agtggtaaat caacgacggt
     8521 tcgattaatt gatggcttac tggaagcaga atccggagag attgtaattg atggccaacg
     8581 tttgactgag gaaaatgttt ggaatatacg tcgtcaaatc ggtatggttt ttcaaaatcc
     8641 agacaatcaa tttgttggag cgactgttga agatgatgtt gcttttggtt tggaaaatca
     8701 gggactttct cgtcaagaaa tgaaaaagag agtagaagaa gctctggctt tagttggcat
     8761 gttggacttt aaaaagagag agccagcgcg tctatcaggt ggccaaaagc aacgtgtggc
     8821 cattgcaggt gttgtagccc taagaccagc tattttaatc ttagatgaag caacgagtat
     8881 gttggatcct gaggggcgta gagaacttat tgggacagta aaaggaattc gaaaagacta
     8941 tgatatgacg gtcatttcta ttacccatga tttggaagaa gtcgccatga gtgatcgcgt
     9001 attggtcatg aaaaaagggg aaattgaatc aactagtagt ccaagggagc ttttctctcg
     9061 aaatgattta gatcaaattg gattagacga tccttttgct aatcaattaa aaaaatcttt
     9121 gagccagaat ggctatgatt tacctgaaaa ttatttgaca gaaagtgagc tagaggataa
     9181 gctatgggaa ttgctctaga aaatgtgaat tttacatatc aagaaggtac tcccttagct
     9241 tcagcagctt tgtcggatgt ttctttgacg attgaagatg gctcttatac agctttaatt
     9301 gggcacacag gtagtggtaa atcaactatt ttacaactct taaatggttt attggtgcca
     9361 agtcaaggga gtgtgagggt ttttgatacc ttaatcacct cgacttctaa aaataaagat
     9421 attcgtcaaa ttagaaaaca ggttggcttg gtatttcagt ttgctgaaaa tcagattttt
     9481 gaagaaacgg ttttgaagga cgttgctttt ggaccgcaaa attttggagt ttctgaagaa
     9541 gatgctgtga agactgcgcg tgagaaactg gctctggttg gaattgatga atcacttttt
     9601 gatcgtagtc cgtttgagct gtcaggggga caaatgagac gtgttgccat tgcaggcata
     9661 cttgccatgg agccagctat attagtctta gatgagccaa cagctggtct agatcctcta
     9721 gggagaaaag agttgatgac cctgttcaaa aaactccacc agtcagggat gaccatcgtc
     9781 ttggtaacgc atttgatgga tgatgttgct gaatatgcaa atcaagtcta tgtaatggaa
     9841 aagggacgtt tagtaaaggg gggcaaacca agtgatgtct ttcaagacgt tgtttttatg
     9901 gaagaagttc agttgggagt acctaaaatt acggcctttt gtaaacgatt ggctgataga
     9961 ggcgtgtcat ttaaacgatt accgattaag atagaggagt ttaaggagtc gctaaatgga
    10021 tagtatgatt ttagggcgtt atatcccagg ggattcgatt gttcaccgat tggatccacg
    10081 tagcaaattg cttgctatga tgctactgat tttgattgtg ttttgggcta ataacccctt
    10141 gacgaatctg attcttttta tagcgacagg gatatttatt gccttgtcag gagtatctct
    10201 ttcatttttt attcagggct tgaagtctat gtttttcttg attgccttca caactatttt
    10261 tcaactattt ttcatttcta atgggaatgt tttatttgag ttttcgtttg tgagaatcac
    10321 ggattatgct ttgcaacaag ctgggattat tttttgtcgc tttgtattga ttattttctt
    10381 ttcaactttg ttaaccttaa caaccatgcc tttaagtttg gcatcagctg tcgaagcttt
    10441 attagcacct ttaaagcgtg tgaaagttcc agttcatgaa attggattga tgctgtctat
    10501 gagtttgcgt tttgtcccaa ccttgatgga tgatacgacg cggattatga atgcacagaa
    10561 agctcgtgga gtggattttg gagaaggaag catcgttcaa aaagtaaagg cgatgattcc
    10621 cattttgatt cctctttttg cgacaagttt aaaacgtgca gattccttgg ctatcgctat
    10681 ggaagcgcgt ggctatcagg gtggaaaagg cagaagtcaa taccgacaat tgaaatggac
    10741 tctaaaggat acgctgacca ttcttgttat tctcgtactt ggttgttgtt tatttttctt
    10801 aaaatcttag tagctttcag gaattgataa aaagttactg taacagtttt tgatataaag
    10861 gtagggatat gaaccgtttt aaaaaatcaa aatatgtcat tattgttttt gtcactgttc
    10921 tgcttgtgtc agctctctta gcgacgactt attcaagtac aattgtgaca aaattaggag
    10981 atggaatctc attggttgat agagttgtac aaaaaccttt tcagtggttt gattctgtca
    11041 aatcagattt ggctcatttg acacgaacat ataatgaaaa tgaaagtttg aagaaacagc
    11101 tttaccaatt agaagttaaa tcaaatgagg tggaaagttt aaagacagaa aatgaacaac
    11161 tgcgccaatt gcttgatatg aagtctaaat tgcaagccac aaagacttta gcagcagatg
    11221 ttattatgcg ttctccggta tcttggaagc aggagttgac cttagatgca ggtagatcaa
    11281 aaggtgcttc tgagaacatg ttagctattg caaatggtgg cttgattggg agtgtttcaa
    11341 aagtagagga gaactctact atagtcaacc ttctgacaaa tacggaaaat gctgataaga
    11401 tttctgttaa aattcaacat ggctctacta caatttatgg aattattatt ggctatgaca
    11461 aggaaaatga cgttcttaaa attagccaat taaatagtaa tagcgatatt agtgcgggag
    11521 ataaggtgac tactggtgga ttaggaaact ttaacgttgc tgatattcct gttggtgaag
    11581 tggttgccac aacgcatagt acagactatt tgacacgaga agtaactgtt aaattgagtg
    11641 cagatactca taatgtagat gtgatagaat tagtggggaa ttcataatga gacagttgaa
    11701 gcgagttgga gtatttttat tgcttccttt ctttgttcta attgacgccc atattagcca
    11761 gcttctgggc tcatttttcc cccatgtaca tttggctagt cattttcttt ttctatttct
    11821 cttatttgag acgatagaag tatcagagta tctctaccta gtctattgtt ttgttatagg
    11881 cttggtttat gatgtttact ttttccatct aatagggatt acaactctct tatttatctt
    11941 attgggagcc ttccttcata aattgaatag tgttattttg ttgaatcgtt ggacaagaat
    12001 gctagctatg attgtgctga cattcctgtt tgaaatgggt agttatcttt tggcttttat
    12061 ggtagggttg acagtagata gcatgtcgat ttttatagtc tatagcttgg taccgacgat
    12121 gattttaaat tttt
//
LOCUS       DAVHIU010000051        12100 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVHIU010000051 DAVHIU010000000
VERSION     DAVHIU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.053"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            26..97
                     /locus_tag="VVN06_001212"
     tRNA            26..97
                     /locus_tag="VVN06_001212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Gln,seq:ttg)
     gene            259..1461
                     /gene="rpsA"
                     /locus_tag="VVN06_001213"
     CDS             259..1461
                     /gene="rpsA"
                     /locus_tag="VVN06_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU5562760.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            1547..2044
                     /locus_tag="VVN06_001214"
     CDS             1547..2044
                     /locus_tag="VVN06_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5562761.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDTMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            2044..3699
                     /gene="dnaX"
                     /locus_tag="VVN06_001215"
     CDS             2044..3699
                     /gene="dnaX"
                     /locus_tag="VVN06_001215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5562762.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTTISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTNLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            3727..3921
                     /locus_tag="VVN06_001216"
     CDS             3727..3921
                     /locus_tag="VVN06_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU5562763.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGCYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            4071..4841
                     /gene="sufC"
                     /locus_tag="VVN06_001217"
     CDS             4071..4841
                     /gene="sufC"
                     /locus_tag="VVN06_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5562764.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            4869..6131
                     /gene="sufD"
                     /locus_tag="VVN06_001218"
     CDS             4869..6131
                     /gene="sufD"
                     /locus_tag="VVN06_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU5562765.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            6142..7368
                     /locus_tag="VVN06_001219"
     CDS             6142..7368
                     /locus_tag="VVN06_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5562766.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            7355..7795
                     /locus_tag="VVN06_001220"
     CDS             7355..7795
                     /locus_tag="VVN06_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5562767.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            7849..9261
                     /gene="sufB"
                     /locus_tag="VVN06_001221"
     CDS             7849..9261
                     /gene="sufB"
                     /locus_tag="VVN06_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5562768.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     VKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(9460..10701)
                     /locus_tag="VVN06_001222"
     CDS             complement(9460..10701)
                     /locus_tag="VVN06_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5562769.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAISAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            10804..11913
                     /locus_tag="VVN06_001223"
     CDS             10804..11913
                     /locus_tag="VVN06_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5562770.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3607 a         2187 c         2657 g         3649 t
ORIGIN      
        1 aatccctctc tctccatttc attaatgggg tatagccaag cggtaaggca agggactttg
       61 actccctcat gcgttggttc gaatccagct accccagttc ttaggtaata atcaagataa
      121 aaagcaaaat atcttagggt attttatttt tataattgaa agacgtgaat gatatgaaca
      181 tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc aagtttaaga aaaacttgat
      241 tgttggagga ttttttagat gaacgaattt gaagatttgc taaatagcgt tagtcaagtt
      301 gagactggtg atgttgttag tgctgaagta ttgacagttg atgcgactca agctaacgtt
      361 gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc gcgaattgac aaacgatcgt
      421 gatgcagata tcaatgactt tgttaaagta ggagaagtat tggatgttct tgtacttcgt
      481 caagtagttg gtaaagatac tgatacagtt acataccttg tatctaaaaa acgccttgaa
      541 gctcgcaaag catgggacaa acttgttggt cgcgaagaag aagttgttac tgttaaagga
      601 acgcgtgccg ttaaaggtgg actttcagta gaatttgaag gtgttcgtgg atttatccca
      661 gcttcaatgt tggatactcg tttcgtacgt aacgctgagc gttttgtagg tcaagaattt
      721 gatactaaaa tcaaagaagt taacgctaaa gaaaaccgtt tcatcctttc acgtcgtgaa
      781 gttgttgaag cagctactgc agcagctcgc gctgaagtat tcggtaaatt ggctgttggt
      841 gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg gcgctttcat cgaccttggt
      901 ggtgttgacg gattggttca cttgactgaa ttgtcacatg aacgtaacgt atcaccaaaa
      961 tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc ttgatcttaa cgaagaagaa
     1021 ggacgtgtat cactttcact taaagcaaca gtaccaggac catgggatgg cgttgagcaa
     1081 aaattggcta aaggtgatgt agtagaagga acagttaaac gtttgactga cttcggtgca
     1141 tttgttgaag tattgccagg tatcgatgga cttgttcacg tatcacaaat ttcacacaaa
     1201 cggattgaaa atccaaaaga agctcttaaa gttggtcaag aagttcaagt taaagttctt
     1261 gaagttaacg cagatgcaga acgcgtgtca ctttctatta aagctcttga agaacgtcca
     1321 gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc gtccacgtcg tccaagacgt
     1381 caagaaaagc gtgatttcga acttccagaa acacaaacag gattttcaat ggctgatttg
     1441 tttggtgata tcgaacttta atcaaattga aaattcacaa aatcctttgt ttactaaaca
     1501 agggattttt cttttgtctc tgctcctttt ttgatataat agttctatgt taaaatcaga
     1561 aaaacaatca cgttatcaaa tgttaaatga agaattgtcc ttcctattgg aaggcgaaac
     1621 caatgttttg gctaatcttt ccaacgccag tgctctcata aaatcacgtt ttcctaatac
     1681 cgtatttgca ggcttttatt tgttcgatgg aaaggaattg gttttaggcc ccttccaagg
     1741 aggtgtttcc tgcatccgta ttgcactagg caagggtgtt tgtggtgagg cagctcactt
     1801 tcaggaaact gttattgttg gagatgtgac gacctatctc aactatattt cttgtgatag
     1861 tctagctaaa agtgaaattg tggtgccgat gatgaagaat ggtcagttac ttggagttct
     1921 ggatctggat tcttcagaga ttgaggatta cgatactatg gatcgagatt atttggaaca
     1981 atttgtcgct attttgcttg aaaagacagc atgggacttt acgatgtttg aggaaaaatc
     2041 ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa acttctccca gttagttggt
     2101 caagaagttg tggctaagac tcttaaacaa gcggtggagc aagagaaaat aagtcacgct
     2161 tatctttttt ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat ctttgccaag
     2221 gctatgaact gtcccaatca agtgggtggc gaaccttgca ataactgcta tatttgtcaa
     2281 gcagtgacgg acggtagttt agaagatgtc attgaaatgg atgcagcttc taataatggg
     2341 gtagatgaaa ttcgcgaaat tcgtgataaa tctacctatg cgcctagcct tgctcgttat
     2401 aaggtttata tcatagatga ggttcacatg ctgtctacag gagcttttaa tgccctccta
     2461 aagacgctgg aagaaccaac acagaatgta gtctttattt tggccactac tgaattgcac
     2521 aagattcctg ctactattct atcccgtgtg caacgttttg agtttaaatc aattaagaca
     2581 caggatatta aggaacatat tcactatatc ttagaaaaag aaaatatcag ttctgaacca
     2641 gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa tgcgggacgc cttgtctatt
     2701 ttggatcaag ccctgagttt gacacaggga aatgagctga cgactactat ctctgaagaa
     2761 attactggca ccattagcct atcagccttg gatgattatg tggcggcctt gtctcaacag
     2821 gatgttccca aagctttgtc ttgcttgaat cttctttttg acaatggtaa gagcatgact
     2881 cgttttgtga ccaatctttt gcactattta agagacttgt taattgttca aacaggggga
     2941 gaaaatactc atcatagttc agtctttgta gaaaatttgg cacttcctca aaaaaatctg
     3001 tttgaaatga ttcgcttagc aacagtgagt ttagcagata ttaagtctag tttgcagccc
     3061 aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa tcaagtccga accagctcta
     3121 tcaggagcgg ttgaaaatga aattgctacg ctgagacagg aagttgcccg tctcaaacaa
     3181 gagctttcta atgtaggtgc ggttcctaaa caagttgcac cagctcctag tcgaccagct
     3241 acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc aatctatctt acaagaggcc
     3301 gtcgaaaatc ctgatttagc acgtcaaaat ttaattcgtt tgcagaatgc ctggggagag
     3361 gtaattgaaa gtctaggtgg gccggacaag gctctgctag ttggttctca accggttgct
     3421 gccaatgaac accatgctat tcttgctttt gagtctaact tcaatgctgg tcaaactatg
     3481 aaacgagaca atctcaatac catgtttggt aatatcctca gtcaggcggc aggtttttca
     3541 cctgagattt tagctatttc catggaggaa tggaaagaag ttcgcgcagc cttttcagcc
     3601 aaagccaaat cttctcaaac tgaaaaagaa gtagaagaaa gcctgattcc agaaggattt
     3661 gaatttttgg ctgataaagt gaaggtagag gaagactaaa gaaagatttc atgatacaat
     3721 aagtttatga ataaacaaca atttattatt atggcgctgt ttacagctgc tgagacctat
     3781 tttttcaatg aagcctggat gactggctgc tatattatgg cagccttttg ggcaatttta
     3841 ctatttagaa atttccgagt cagttatgtg atgggcaaaa tcgttgatgt catcgatcag
     3901 cattttaata ggaaagacta gccctcagct tccagacaaa atcaaagcct tttaggcttt
     3961 tttttgttat actagaaaag tatatttata gaatttttgc tctatttctg gggaaatcag
     4021 acgtttttct agtaagtact gtaaaagttt tgaaaaagaa aggaactatc atgtcagtaa
     4081 tagagatcaa agatcttcac gttgagattg aaggaaaaga aattttaaaa ggggttaacc
     4141 tgaccctgaa aacaggagaa attgccgcta tcatgggacc aaatggtaca ggtaaatcga
     4201 ctctttctgc cgctatcatg ggaaatccaa actatgaagt aactaaaggt gaagttttgt
     4261 ttgatggcgt aaacatcctt gagttggaag tggatgagcg tgcgcgtatg ggacttttcc
     4321 ttgctatgca atacccatca gaaatccctg gaattaccaa tgctgagttt cttcgtgccg
     4381 ctatgaatgc gggtaaagaa gatgatgaga agatttcagt tcgtgagttt attactaagc
     4441 tagatgaaaa aatggaattg ctcaacatga aagaagaaat ggcagagcgt tacctcaacg
     4501 aaggcttctc tggtggtgag aaaaaacgca atgaaattct tcaacttttg atgttggagc
     4561 caacatttgc tcttttggac gagattgact caggtcttga tattgacgct cttaaagttg
     4621 tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg tgctatgatc atcactcact
     4681 accaacgtct tttgaactat atcacacctg atgtggtaca cgtgatgatg gaaggtcgtg
     4741 ttgtcctttc tggtggtcca gaattggctg cgcgtttgga acgtgaagga tacgcaaaat
     4801 tagctgaaga acttggctac gactacaagg aagaattgta attccctcgt atcttttagg
     4861 agaagtaaat gactagagaa aatattaaac ttttttcaga aatgcacgct gaaccaagct
     4921 ggttggctga tctccgtcaa aaagcttttg acaagattga gactttggaa ttaccagtta
     4981 ttgagcgtgt caaattccac cgttggaatc tgggtgatgg aacgattaca gaaaatgagc
     5041 catcagcaaa tgttccagat ttcacagctt tagatcatca cttgaagttg gtgcaagtag
     5101 gaactcaaac tgttttcgaa caaactccag ttgagttagc tgaacagggt gttgtcttca
     5161 cagactttca ctcagcttta gaagaaattc cagagctgat cgaagaattc ttcatgtcat
     5221 ctgttaagta tgatgatgac aagttggcgg cttaccacac agcttacttt aacagtggtg
     5281 ctgtactcta tattccagat aacgtagaaa ttacagagcc aattgaagga attttctacc
     5341 aagatagcga tagcaatgtg ccgtttaaca agcatattat gattatcgct ggtaaaaatt
     5401 ctaagattag ttatctggag cgtttagagt cacgcggtga aggaagtgac aaagcaactg
     5461 ccaatatcac agtggaagtg attgcacgtt ctggtgcgca agtcaagttt gctgctatcg
     5521 accgtctagg tgaaaacgtc actgcctaca ttagccgtcg tggtaaatta ggcaacgatg
     5581 caagtattga ctgggctatc ggtgtcatga acgaaggaaa tgtcgttgct gattttgata
     5641 gtgacttgat tggtaatggt agccatgctg acctcaaggt tgtagctctt tcaagtggtc
     5701 gtcaggtaca agggattgat actcgtgtaa ctaactatgg ctgcaactca atcggaaaca
     5761 ttctacaaca tggggttatc cttgaaaaag caactttgac tttcaatggt atcggccaca
     5821 tcatcaaggg tgctaaggga gcagatgcgc aacaagagag ccgtgttctc atgctttcag
     5881 accaagcgcg ttcagatgct aacccaattc ttttgattga tgaaaatgac gtaactgcag
     5941 gacatgcagc ctctattggt caggtagatc cagaagacat gtattacctc atgagtcgtg
     6001 gattggataa ggcaactgca gagcgtttgg ttgttcgtgg tttccttgga tctgttatcg
     6061 tggagattcc agtcaaggaa gttcgtgatg aaatgattgc aactatcgaa gagaaattgt
     6121 caaaacgcta aggggaagcc tatgttagat gtagaagcga ttcgcaagga ttttccaatt
     6181 ttagatcaga ttgtcaatga tgaacctctg gtctatctgg acaatgctgc gacgacacaa
     6241 aaaccactag tagttctgaa agctattaac agctactatg agcaggacaa tgccaatgtt
     6301 caccgtggtg tccatacctt agcggaacga gcgacagctt cttatgaagc tgctcgtgaa
     6361 accattcgta agtttattaa tgcaggctct acaaaggaag ttctctttac cagaggaacg
     6421 acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa ttctcgctga gggagaccag
     6481 gtcttgattt cagtaatgga acaccattct aatatcattc catggcagga agcttgtcga
     6541 aagactggag cagagcttgt ctatgtctat cttaaagacg gtgccttgga tatggaggat
     6601 ttgcgagcta aattgactga taaggttaaa tttgtttccc tagctcatgc ctccaatgtt
     6661 cttggtgtgg tcaatccgat caaggaaatc actcaattag cccaccaagt tggggcaatt
     6721 atggtagtgg atggtgctca atctacacct catatgaaga ttgatgtcca ggacttggat
     6781 ctggactttt tcgccttttc gggtcacaag atggctggtc cgactggtat cggtgtcctt
     6841 tacggcaaag aaaagtatct tgagcaaatg tctccagtag aatttggcgg cgagatgatt
     6901 gattttgtct acgagcaatt tgctagttgg aaggaattgc cttggaaatt tgaggctgga
     6961 acgccaaata tggcaggagc tattggactt gcgactgcag ttgattatct ggaaaagatt
     7021 ggtatggatg ccgttgaagc tcatgaacag gaattgattg cgtacgtcta tccaaaactg
     7081 caggcaattg agggattgac catttacggt tctcaggatt tggctcaacg ttcgggtgtt
     7141 attgccttta acctaggtga tctccatcct cacgatcttg cgacggctct ggattatgaa
     7201 ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct tgcttcagta tttggaagtc
     7261 ccagcaacag ctcgtgcaag tttttatatc tacaatacca aggcagattg cgacaaacta
     7321 gtcgatgccc tacaaaagac aaaggagttt ttcaatggca ctttctaaac tagatagcct
     7381 ttatatggca gtggtagcag accattcgaa aaatccacat caccaaggga agttagaaga
     7441 tgctgagcaa atcagtctca acaatccgac ttgtggggat gtcatcaacc tctctgtcaa
     7501 gtttgatgca gaggaccgtt tggaagatat tgcttttcta aattcaggat gcacgatttc
     7561 aactgcttct gctagtatga tgacagatgc cgttttagga aaaaccaaac aagaaatttt
     7621 agaactggcg actatttttt ctgaaatggt tcaagggcaa aaagatgagc gtcaaggcca
     7681 acttggagac gcggcattct tgtcaggtgt tgccaaattc cctcaaagaa tcaagtgtgc
     7741 aaccctagct tggaatgccc ttaagaaaac aattgaaaat caagaaaaac agtaagacaa
     7801 gtttcttttg tcttatgaat tattagaaat gaagaaagaa aggatactat ggctgaagaa
     7861 agagtagaac caaaaccaat tgaccttggt gaatataaat ttggtttcca tgacgatgta
     7921 gagcctgtct tatcgacagg aaaaggactc aacgaaggtg ttattcgtga attatctgct
     7981 gttaagggtg agcctgagtg gatgttggag ttccgtttga agtcttatga aaccttcaaa
     8041 aaaatgccca tgcaaacttg gggagcagac ttgtcagaga ttgactttga tgacttaatc
     8101 tactaccaaa aaccatctga caaaccagcc cgttcttggg atgatgtacc tgaaaagatt
     8161 aaagaaacct ttgaacgtat cgggattcca gaagctgaac gtgcttattt agcaggggct
     8221 tctgcccagt acgagtcaga agtggtttac cacaacatga aggaagagtt ccaaaaatta
     8281 ggtattatct ttacagatac agattccgca ctcaaggaat acccagactt atttaaacaa
     8341 tactttgcga agttggtacc gccgacagat aacaagttgg cagccctcaa ctcagcagta
     8401 tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca aggtagatat tccacttcaa
     8461 acttatttcc gtatcaataa cgaaaatata ggtcagttcg aacgtacctt gattatcgtt
     8521 gatgagggag caagcgtcca ctacgtagaa ggatgtacag caccaacata ttcaagcaat
     8581 agcttgcacg ctgccattgt agaaattttt gctttggacg gagcttatat gcgttataca
     8641 actatccaaa actggtctga taacgtctat aacttggtaa caaagcgtgc taaggctcaa
     8701 aaggatgcca ctgttgagtg gattgatgga aacttgggtg ccaaaacgac tatgaaatat
     8761 ccatctgttt accttgatgg agaaggagcg cgtggtacca tgctctctat cgcctttgct
     8821 aatgcagggc aacaccaaga cacgggtgct aagatgattc acaatgctcc acataccagc
     8881 tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa aggttgacta ccgtggacaa
     8941 gtcaccttta acaagaactc taagaaatct gtttcccaca ttgaatgtga taccattatc
     9001 atggatgact tgtcagcatc agatactatt ccatttaatg aaattcacaa ctcgcaagtg
     9061 gctttggaac acgaagccaa agtatctaag atttcagaag agcaattgta ttatctcatg
     9121 agccgtggat tgtcagaatc tgaggcaact gaaatgattg tcatgggatt tgtagaaccc
     9181 tttacaaaag aacttccaat ggaatacgca gttgagctga accgcttgat tagctatgaa
     9241 atggagggat cagttggata aaatttgatt ttatactctt cgaaaatctc ttcaaaccac
     9301 gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca
     9361 aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt gttctttgat tttgaagatg
     9421 aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa
     9481 acggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc
     9541 taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc
     9601 ttcataagtc aaatggccaa ccactgttcc agcttcaagc ggtgctgaga ttgctttgga
     9661 atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcga ttagatagat
     9721 atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct
     9781 atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata
     9841 atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc
     9901 atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc
     9961 tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat
    10021 accttctaac atgtagttgg ttgaagtgat tgtcattcca gcaaaagtag aagaaggttt
    10081 tttggtgatt tccaagactt gtgggtattt tttgatgagg ttgcgagcaa caatagcgac
    10141 atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc
    10201 tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc
    10261 caagagtttt gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc
    10321 tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac
    10381 agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata
    10441 aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc
    10501 atagaccaga taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc
    10561 atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc
    10621 aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa
    10681 agttaaaaat atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg
    10741 ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa
    10801 ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc
    10861 atcttttacc tatggagaca gatgggatct ttgattaacc cttttgttag cgtgcttaat
    10921 acaattatga ttccattttt attagggggc tttctttatt atttgacaaa ccctattgtt
    10981 actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt
    11041 actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag
    11101 ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata
    11161 gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta
    11221 aacttatcct atgttgatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg
    11281 agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg
    11341 gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag
    11401 agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat
    11461 attagtggag tttcgattga cgcaatcatt ataggttgtt tagcttatat tggctatagt
    11521 attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt
    11581 ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat
    11641 ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat
    11701 atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt
    11761 ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc
    11821 tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata
    11881 atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt
    11941 actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc
    12001 acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattccgtg
    12061 tcagggtaag ttccactggt tttcatagtc tattaaagtt
//
LOCUS       DAVHIU010000052        11828 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVHIU010000052 DAVHIU010000000
VERSION     DAVHIU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.054"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(83..1102)
                     /gene="adhP"
                     /locus_tag="VVN06_001224"
     CDS             complement(83..1102)
                     /gene="adhP"
                     /locus_tag="VVN06_001224"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU5562771.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIATADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1441..2253)
                     /locus_tag="VVN06_001225"
     CDS             complement(1441..2253)
                     /locus_tag="VVN06_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5562772.1"
                     /translation="MTKKIIALDLDGTLLNSDSQISDFTKETIKKVTEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2443..3861
                     /locus_tag="VVN06_001226"
     CDS             2443..3861
                     /locus_tag="VVN06_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU5562773.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALISDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTSIGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMAEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            3966..4673
                     /locus_tag="VVN06_001227"
                     /pseudo
     CDS             3966..4673
                     /locus_tag="VVN06_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            4918..5898
                     /gene="folP"
                     /locus_tag="VVN06_001228"
     CDS             4918..5898
                     /gene="folP"
                     /locus_tag="VVN06_001228"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5562774.1"
                     /translation="MVKSLEGVTDMSSKTNHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAHEVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEKELADFEKLPIEELMETFFERALARAEEAGIAQENILLDSGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5900..7222
                     /locus_tag="VVN06_001229"
     CDS             5900..7222
                     /locus_tag="VVN06_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU5562775.1"
                     /translation="MKEIQNNQWIANYRTDQPHFGLERMVELLALRGDPHLKLKVIHV
                     GGTNGKGSTIAFLKNMLEKMGLRVGVFSSPYLIHYTDQISINGESIPEARLESLMVDY
                     QSLLVGEVSTKLQETTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRITPEALTVIDRIAEGKVAPR
                     LAYGADYQVCHQESVVTGEVFDYTSSVRQGRFQIGLLGLHQIENAGMAIALLDTFCQE
                     DGRELVANILIAQALEETSWPGRLEVVSRDPLMILDGAHNPHAIKALLATLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFDDSRATAESVLEEAAKSRNLSYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRTYLMERKNENGYTKD"
     gene            7203..7757
                     /gene="folE"
                     /locus_tag="VVN06_001230"
     CDS             7203..7757
                     /gene="folE"
                     /locus_tag="VVN06_001230"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5562776.1"
                     /translation="MDTQKIEAAVKMIIEAVGENVNREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7800..8612
                     /gene="folK"
                     /locus_tag="VVN06_001231"
     CDS             7800..8612
                     /gene="folK"
                     /locus_tag="VVN06_001231"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5562777.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLET
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8657..8974)
                     /locus_tag="VVN06_001232"
     CDS             complement(8657..8974)
                     /locus_tag="VVN06_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU5562778.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9330..9776
                     /gene="rplM"
                     /locus_tag="VVN06_001233"
     CDS             9330..9776
                     /gene="rplM"
                     /locus_tag="VVN06_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5562779.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9795..10187
                     /gene="rpsI"
                     /locus_tag="VVN06_001234"
     CDS             9795..10187
                     /gene="rpsI"
                     /locus_tag="VVN06_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5562780.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10295..11515)
                     /locus_tag="VVN06_001235"
     CDS             complement(10295..11515)
                     /locus_tag="VVN06_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5562781.1"
                     /translation="MTVTKTNSNTYNLKVYIPKEIRPKMGIKGNHFVKRYKTRKEAKE
                     AELEILTKIHQLENGEDILLSKTNDILFSEFFNNIWWDSYKAGQTTSTTRPPTQVTID
                     NTKTVFRKHILPMFGNYSINFLNQNKQVVLNLMTAKAEEYANFKVIRSYVNSIFDWAE
                     ELEYIESNRLSKTIKRIKATKKLKLQESKNEEDLYLSSEELQDWFDAFREDLENDKLS
                     LKDYVLFFTTFILSDRKSESYALHWKNIDLENAQIDLRHALDKYKNVKSTKGNKKTIF
                     SIPSYLVSLLSEWKKQQKYELAKFGIMQTSDQLVFTYIDTKGNVNSPLHVDYLNNKMN
                     SVKRRHPKLKHATPHKLRHTGATLAKKAGMSLEAISEALTHSDTITTKTYVNTSNIVP
                     LSAGQVAYQHLKNK"
     gene            complement(11557..11826)
                     /locus_tag="VVN06_001236"
     CDS             complement(11557..11826)
                     /locus_tag="VVN06_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562782.1"
                     /translation="MQVILPTEQVQEIQSMITNLLQNEISHFKEDLGLDSPYLNKGQT
                     CKYLGISNNTLDGWIQKGLPVIKIGKTVRFNKNEIDKWLCRKAML"
BASE COUNT         3383 a         2226 c         2570 g         3649 t
ORIGIN      
        1 gagtaatctt gaattcttat ttaaaattta atataaaaaa gttggaagaa gcttccaact
       61 tttaaaatag gtttctatta aattagtggg tgaagtcgag taccatacgt ccttggattt
      121 ggcctttttc catttcgtcg aaaatggcaa cagcatcttc tactggacgt ttttgaacaa
      181 ctgggactac cagaccttct gcaccaaatt ggaaggcttc ttctaagtct ttacgagttc
      241 cgacaagaga accgatgact tggattccat cgaggactgt tttaacgatg ctgagttcca
      301 tcatttcaga aggaagacca acagcgacga cgcgaccacc agcacgaatg gagtcaacag
      361 cctggttgaa ggcaacttta gacacagcag ttacgacagc tgaatgagct cctccatcag
      421 ttttttcttt aatgagtcca gctacatctt caacttcgag gccgttaatc acaatgtcag
      481 cgcctacttc ttttgcaagg gtaagtttgt cattgttgat atcgactgcg ataacatgag
      541 cattgaatac ttttttagcg tattgaacag cgaggttacc aagtccacca gcaccgtaaa
      601 gaacaaccca ttggcctggt tcaacttttg cttctttgat agctttatag gttgttactc
      661 cagcacatgt gatagaagaa gcttgggctg gatcaagtcc gtcaggaact ttgacagcat
      721 agtcagcagt tgcgatacat tgttcagcca taccaccgtc tactgagtag ccagcatttt
      781 tcactgtacg gcaaagggtt tcgcgaccag ttgtacagta ttcgcaagtg ccacatcctt
      841 caaagaacca agcaacgctg acgcggtcac cgactttaag gcttttcaca tctggagcaa
      901 tctctttaac gataccgata ccttcgtgcc caagaacacg tcctgggact tgaccaaagt
      961 caccatgagc aacgtggagg tcggtgtggc aaacgccaca gtattcaact tctacaagtg
     1021 cttccccagt ttcaagtgga cggagtactt tttcttcaat agcaacacca gtgctttctg
     1081 gatttacaac aacagctttc atagctatcc tccttgatat tgaccgagaa cagggacaac
     1141 gggactggat tgattttttg tcaacagagt cgagtgtgac gagctctctt gtatttatat
     1201 gtgaagtata tcacaaaagg aagccttttc caaggttttg gagacaaaaa gtagaacaat
     1261 cgattaactg gttgatgaga ctgtgaaaag agcacaaaga ccagcttgca agctggtcat
     1321 actcttcgaa aatctcttca aaccacgtca gcttcatcta caacctcaaa gcagtgcttt
     1381 gagcaaccta cggctagctt cctagtttgc tctttgattc tcattgagta tcagtatagg
     1441 ttataagaat aagtcttgta gggttttggc aaccccatct tggtcgttgg taagggaaat
     1501 ttgctcatct gcataaggga gtagctctgg attggcattt ttcatggcat aacccttccc
     1561 agcaaaagcg agcatttcgg tatcattgtg ttcatctcca aaggcaatca aatctttttt
     1621 gtcacgattc attatcttga gcaagtagtc caaagcaaag gccttgttga caccttttgg
     1681 ggtacattca aggatattga gcggacctcc ccaggtattg atagaaagtt gatgctggta
     1741 gaaggcgttc atgtcttttg ccaaggcata tttgtcactg gctctggtct gcaagaggat
     1801 acagttaggg tccttggtca ccaattcagg ctggaattga tcttcaggct ggaaagcttc
     1861 tacaccaaat agtttgggat tggcaatttc ttcattggga tttgtaatgt agaatttttt
     1921 acgatattct ccagcgataa aatcggcttg aatgtcctct gaacgttgaa ccatatctag
     1981 cagatatttt ttgtctacag tcaaacactt ttcaaaatcc caaacttggt ctggtaaatg
     2041 agtaagggat ccgttgaagt taatcatagg agtgtctaag cccagttcac ggtaaaaatc
     2101 ttttgacatg cggtaagggc gacctgtcgt aataataacc tgatggcctt tttcagtaac
     2161 ttttttaatg gtttctttgg taaagtcaga aatctgactg tctgagttga gcagggttcc
     2221 atccagatct agtgcaataa tttttttcgt catataaacc tttctagtgt ctttccagat
     2281 aagatgtctt ctattataac aaaaagcaga cagaaaagca gattccaaac aaaaaatgaa
     2341 attccatcaa tttcttaaat aaattaaaca aagatattga aaaagtgaat gataaatgat
     2401 ataatacaga tattgttcgt aaaaattaaa aggagattga taatggacaa attatttaaa
     2461 ctaaaagaga acggtacaga cgttcgtaca gaggttctcg ctggtttaac aactttcttt
     2521 gcaatgagct atattctctt tgtaaaccca caaattcttt cgcaaacagg gatgcctgct
     2581 cagggtgtct tcctcgcaac gattattggt gcagtagcgg gtaccttgat gatggctttt
     2641 tatgctaact tgccatacgc tcaagcgcca ggtatgggac tgaatgcctt ctttaccttc
     2701 accgtcgtat ttggacttgg ttattcttgg caagaagcct tggctatggt cttcatctgt
     2761 gggattatct cattgattat taccttgaca aatgttcgta aaatgatcat tgaatcgatt
     2821 ccaaatgccc ttcgctcagc tatttcagct ggtatcggtg tctttcttgc ctacgtggga
     2881 attaagaatg ctggactttt gaaatttacg attgatccag gcaactatac tgttgtggga
     2941 gaaggggctg acaaggctca agcaacgatt gcagcaaact cttcagcagt tccaggattg
     3001 gtcagcttta ataatccagc tgttttggtg gctcttgcag gacttgccat tactatcttc
     3061 tttgttatca aggggattaa agggggaatt attctttcta tcttgacaac aactgttctt
     3121 gctatcgcag ttggtttggt agatttgtct agtatcgatt ttgctaataa ccatgttggt
     3181 gcagcttttg aagatttgaa gacaatcttt ggtgcagctc ttggttcaga aggtttggga
     3241 gctttgattt cagatacagc tcgcttacca gaaactctta tggcaattct tgccttctcg
     3301 ttgacagata tttttgatac aattggtact ttaatcggta ctggtgaaaa agttggtatc
     3361 gtagcaacaa atggtgaaaa tcaccaatca gctaagttgg acaaggctct ttactctgat
     3421 ttgattggta cttctattgg tgccatcgca ggtacttcaa acgtaacgac ttatgttgag
     3481 tctgctgctg gtattggtgc aggtggacgt acaggtttga cagccttggt tgtggcaatc
     3541 tgttttgcaa tctcaagctt ctttagccca cttctagcta tcgtaccaac agccgctaca
     3601 gctccaatct tgattatcgt cggaattatg atgttggcta gcttgaaaaa tatccattgg
     3661 gatgatatgg ctgaagcggt tcctgctttc ttcacatcta tctttatggg attcagctac
     3721 tctatcactc aagggattgc agttggtttc ttgacttaca ctttgactaa gcttgtcaaa
     3781 ggtcaagcta aagatgttca tgtaatgatt tggattttgg atgccttgtt tatccttaac
     3841 tacattagca tggcactata aatgctataa tataaaaagg gggtctcccc tttttatatt
     3901 atagttactt gaaaattgct tcaaactagg taggtgattt tgattgaatg gtaggagtat
     3961 aattgatgat aaaaaagaat atttgggatg aagtattaaa cagggggaag tggttaataa
     4021 tctttctagt atggcttttg ttttctcagt ttcctttagc cctagcaact tttctaacta
     4081 gcagaaaatt tccactgctt caaactggac tattagtagg agccttatct cttgtgattt
     4141 taactgtatt tataataggt gctaggaaaa cacagttggc tagttttggt ttatcgtttt
     4201 tcaaggctaa ggatttggct agattggcct taagctatct agtgattctt gcctttaata
     4261 ttcttggtgt ggtcttactg cgtttgatga atgaaacaac aaccagcaac caatcaaaca
     4321 ttaatgatct ggttcagaat agttctctga tttccagttt cttcttgttg accttgcttg
     4381 ctccgatttg ttaggaaatc ttgtgccgtg ggattgttcc taaaaagatt ttccgaggca
     4441 aggagaactt gggatttata gtaggtatga ttgtgtttgc tttattgcac caaccaagta
     4501 atttaccttc tttattgatt tatggaggta tgtccacggt tctatcttgg acggcttaca
     4561 agacccaacg tttggaaatg tctatcttac ttcacatgat tgtgaatggg gttgttttct
     4621 gtttgttagc tcttgtagtg atcatgagtc ggacattagg gatttctgtt taatactcaa
     4681 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaaacac tgttttgagg
     4741 ttgtagataa gactgacgaa gtcagtcaca tatatacggc aaggcgacgt tgacgcagtt
     4801 tgaagagatt tttgaagagt ataagactat tgacaattgc ttacttgttt ctactgggaa
     4861 aaagatgaat gtaatcgtgt ccattttttt ctttttatgg taaaatagag aaataatatg
     4921 gtgaaaagcc ttgagggagt gaccgatatg tcaagtaaaa ccaatcatgc aaagacagct
     4981 atttgcggta ttatcaatgt aaccccagac tccttttcgg atggtggtca attttttgct
     5041 cttgagcagg cactccagca ggctcgtaaa ttgatagcag aaggagccag catgctagat
     5101 atcggcggag aatcgactcg gccgcggccg ggaagtagtt atgttgagat agaagaggaa
     5161 atccagcgtg ttgttccagt gatcaaagct attcgcaagg aaagtgatgt cctcatctct
     5221 attgatactt ggaagagtca agtagcagaa gctgctttgg ctgctggtgc caatctagtc
     5281 aatgatatca ctggtcttat gggtgatgag aaaatggccc atgaggttgc taaggctggt
     5341 gcgaaagtag tcattatgtt taatccagtc atggcacgac ctcagcaccc tagctcgctc
     5401 atcttccctc attttggctt tggtcaagct tttacagaaa aagagttagc tgactttgaa
     5461 aaattgccaa tcgaagagtt gatggagact ttttttgaac gagcactagc gagagcagag
     5521 gaagctggga ttgctcaaga aaacatcctg ctggattcag gaattggctt tggtctgacc
     5581 aagaaagaaa atctgcttct tttacgggat ctggataaac tacatcagaa aggctatcca
     5641 atctttctcg gagtgtcgcg caagcggttt gtcatcaata tccttgaaga aagtggtttt
     5701 gaagtcaatc ctgagacaga acttggtttc cgaaatcggg acacggcttc ggctcatgta
     5761 acaagtatcg ctgcgagaca gggtgtagaa gtggtgcgcg tgcatgacgt agctagtcac
     5821 aggatggcgg ttgaaattgc ctccgctatt cggcttgctg atgaagcgga aaatttagat
     5881 ttaaaacaat ataaataaga tgaaagaaat tcaaaacaat cagtggattg ccaactaccg
     5941 gacggaccaa ccgcattttg gcttggaacg aatggtggaa ctgttagcct tgcgtggaga
     6001 tccccatctc aaactcaagg tcatccatgt cggagggacc aatggcaagg ggtcgaccat
     6061 tgctttttta aaaaatatgc tagaaaagat ggggctgaga gttggtgttt tcagctcgcc
     6121 ctatctcatt cattacacag accagattag catcaatggg gaatccattc ccgaggctag
     6181 actggaatcc cttatggtag actatcagtc tttgctggtg ggggaagtgt ctaccaagtt
     6241 gcaggagaca accgagtttg agattatcac agctctggct tatgactatt ttgcctcaga
     6301 gcaagtagat gtggctatca tggaagtggg catgggtgga cttttagata gtaccaatgt
     6361 ttgccagcct attttaacag gcatcacaac cattggactg gaccatgtag ctctacttgg
     6421 tgataccttg gaagctatag cagagcagaa agctggtatt atcaaacaag gaattccctt
     6481 ggtgacaggt cgtatcactc cagaagcctt gactgtgatt gaccgcattg cggaagggaa
     6541 agttgcgccg agacttgcct acggagcaga ttatcaggtt tgtcatcaag aaagtgtagt
     6601 gacaggggaa gtctttgact ataccagttc tgtcagacaa gggcgcttcc agattggcct
     6661 gcttggtttg caccagatag agaatgctgg gatggccata gctttacttg atactttttg
     6721 tcaagaagat ggtcgagagc tagtagctaa tatcttgatt gctcaagctc tagaagaaac
     6781 aagttggccg gggcgcttgg aagttgtgtc tagagacccc ttgatgattt tggatggagc
     6841 ccataatccc catgctatca aggctttatt ggcaaccttg caagaacgct ttgcggatta
     6901 tcacaaggaa atcctcttta cttgcatcaa aaccaaggcc ttagaggata tgttggactt
     6961 gctgggggcc atgccagata ccgagcttac cctgacacat tttgacgaca gtcgggcgac
     7021 tgctgaaagc gtgctggaag aggcagccaa gtctagaaat ctcagctacc aaggttggca
     7081 ggattttcta gagcagaaat tgacagataa aaaagaagag aaaaaaacag ttaggattgt
     7141 cacgggttcc ttgtatttct tgagccaagt gagaacctat ctgatggaga ggaagaacga
     7201 gaatggatac acaaaagatt gaagcagctg taaaaatgat tatcgaggct gtaggagaga
     7261 acgttaatcg cgagggcttg caggaaacac ctgctcgtgt agcccgtatg tatcaagaga
     7321 ttttttcagg tcttggtcaa acagcagagg aacatttgtc aaaatccttt gaaattattg
     7381 acgataatat ggtggtagaa aaggatatct ttttccatac catgtgtgaa caccacttct
     7441 tgccatttta tggtagagcg cacattgcct acattccaga tggtcgtgtg gcaggcttgt
     7501 ctaagctagc ccgtacggtt gaagtttatt cgaaaaaacc acaaattcaa gaacgtttga
     7561 atatcgaagt ggccgatgcc ttgatggact atctaggtgc taaaggagcc tttgttgtca
     7621 ttgaggcgga acatatgtgt atgagtatgc gtggtgttag aaaaccaggc actgcaacct
     7681 tgacgacagt agctcgtggt ctatttgaaa cagataagga tctccgtgac caagcttatc
     7741 gtttaatggg gctataaaaa gaatccgctt caagcggatt tttctagaaa ggaatcatta
     7801 tggatcaact gcagattaag gatttggaaa tgtttgccta tcatggtctt tttcctagtg
     7861 agaaagaatt ggggcagaaa tttgtcgttt cagccatcct atcctatgat atgaccaagg
     7921 cagctacaga cttggattta acagcctctg tccattacgg agaattgtgt cagcagtgga
     7981 cgacttggtt tcaggaaacg agtgaagatt tgattgaaac ggtagcctat aaactggtgg
     8041 aacgtacctt tgagtcttat cctcttgtcc aagaaatgaa gttggaactg aaaaaacctt
     8101 gggcaccggt gcatttgtca ctagatactt gctcggtaac cattcatcgc cgcaagcaac
     8161 gagcctttat cgccctagga agcaatatgg gagataaaca agtaaacttg aagcaagcca
     8221 ttgacaaact gcgagctcgt ggcatccata ttctcaaaga gtccagtgtc ttagagacgg
     8281 agccttgggg tggagtggag caggatagct ttgccaatca agtggttgag gtggaaacct
     8341 ggctaccagc acaagacttg ttagaaacct tgttagccat tgagtcagag ctgggacggg
     8401 tgagagaagt gcattgggga cctcgtttga ttgatttgga cttgctcttt gtggaggacc
     8461 agatccttta tacagacgac ctcatattgc ctcatcctta catagcggaa cgcctttttg
     8521 tccttgagtc cttacaggaa attgcgcctc attttatcca tccgatatta aaacaaccga
     8581 tccgcaactt gtatgatgct ttgaaaaaat agaaaaactc tagttttcag ttacttgcaa
     8641 ctgaaggcta gagtttttaa aataggtcat tttcttctgg gaggaggata gtttctctac
     8701 cgtccatgtc taaaaccagt actcttgggg gataacgagg gtcgaaagga tggttaaagt
     8761 caaaatcaat ggctgtaggg aggtgttgac ttgaaaagtg gaaggtaatc tttccttggt
     8821 tattaagcaa ttgaaactcg agttcttctt ccaattcaaa gacatttttt aagaaatgat
     8881 cgatgatata ccaaaaagag tcaatgatgt catcgggcaa gctggtaaca atgccaaaac
     8941 tagcagaccg catgtgggta ttggtaaaag ccatatctct gtcccctttc ttttccctta
     9001 tcatacagca aataggatta aaaatcaaga aaaggtgatt ttttgaaaag gattttagtt
     9061 acagggagaa atagggaaaa aattcccaaa aatctaccga agttaatagg taaattccca
     9121 aattaacttg attatataac tttcagttat tttgagaagt taccgaaaaa tattttttat
     9181 atctattgac ttttaggggt gaaatttggt atgatagtag gcggtattgt ttaccccatt
     9241 tgaaaggccc cggaaccttc caaatacttt tcgatgggaa ggaacaccca tcaccgtaaa
     9301 caaaaatcga actatatata ggagaaatca tgaacaaaac aacatttatg gctaaaccag
     9361 gccaagttga acgtaaatgg tacgtagttg acgcaactga tgtaccactt ggacgtcttt
     9421 ctgcagtagt tgctagcgta cttcgcggaa aaaacaaacc aacatttaca ccacacactg
     9481 atacaggtga cttcgtgatt gttatcaatg ctgaaaaagt taaattgact ggtaaaaaag
     9541 caactgataa aatctactac actcactcaa accacccagg tggattgaaa caaatctctg
     9601 caggtgaact tcgttctaaa aatgcagtac gtttgattga gaaatcagtt aaaggtatgc
     9661 ttccacacaa tacacttgga cgcgctcaag gtatgaagtt gaaagtattt gttggagctg
     9721 agcacactca cgctgcacaa caaccagaag ttcttgacat ttcaggactt atctaaggaa
     9781 aggaacaata aagtatgtca caagcacaat atgcaggtac tggacgtcgt aaaaacgctg
     9841 ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac tgttaacaaa aaagatgttg
     9901 aagagtacat cccacacgct gaccttcgtc ttgtcatcaa ccaaccattc gcagttactt
     9961 caactgtagg ttcatacgac gttttcgtta acgttatagg tggtggatac gctggtcaat
    10021 caggagctat ccgtcacggt atcgctcgtg cccttcttca agtagaccca gacttccgcg
    10081 attcattgaa acgcgcagga cttcttacac gtgactcacg taaagttgaa cgtaagaaac
    10141 caggtcttaa gaaagctcgt aaagcatcac aattctcaaa acgttaatca actcgattat
    10201 atcaacgttt cgaagcactc acaggtttgc cctatgggtg cttttttcta tactttctac
    10261 aaaagtttgc cctaaaattt gccctaaatt ttccctactt gtttttaaga tgttggtagg
    10321 caacttgtcc agcagataat ggaactatgt ttgaagtatt aacataagtc ttcgttgtaa
    10381 ttgtatcgct atgagttaat gcttcagaaa tggcttctaa gctcattcct gcttttttag
    10441 caagtgtcgc tccagtatgt cgtaatttat gaggtgtagc gtgttttaac tttgggtgtc
    10501 gtcttttaac agagttcatt ttgttattga gataatcaac gtgtagaggg ctatttacat
    10561 tacctttcgt atctatatag gtaaagacta gttgatcgga tgtttgcatt ataccaaatt
    10621 tcgctaactc atatttctgc tgttttttcc attctgaaag aagtgataca aggtaactag
    10681 gaattgaaaa aatcgttttc ttatttcctt ttgttgactt tacattctta tatttgtcaa
    10741 gtgcatgcct taagtcaatt tgagcgtttt ctaagtctat gtttttccaa tgtagggcat
    10801 aggattcaga ttttctatcg cttagaataa aggttgtaaa gaataggaca taatctttta
    10861 gtgataattt atcgttctcc aaatcttctc taaaagcatc aaaccaatct tgtaattctt
    10921 cactagatag atacaaatct tcctcatttt tggattcttg gagttttagc tttttggtag
    10981 ctttaattcg tttaattgtc ttggataatc gatttgattc tatgtactct aattcctcag
    11041 cccaatcgaa aattgagttt acataacttc gaataacctt gaaattagca tattcttcag
    11101 ctttagcagt cattaagttt agtacgactt gcttattctg attcagaaaa ttgattgaat
    11161 aattaccgaa cattggtaag atatgctttc tgaaaacagt tttagtatta tcaatagtaa
    11221 cctgtgttgg tggtctagtt gttgaagttg tttgaccagc tttgtatgaa tcccaccaaa
    11281 tattattaaa gaactcagag aatagaatat cgttagtttt agaaagtagg atatcctcac
    11341 cattttcaag ttgatgtatc ttagttaaaa tttctagttc agcttctttg gcctcctttc
    11401 ttgttttata gcgtttaaca aagtgatttc ctttaatccc cattttaggt cggatttctt
    11461 taggtatata cactttaaga ttgtaagtgt tactatttgt ttttgtaact gtcatgatgt
    11521 tctcctttta cgagtcagga tatatcatta gagatattat agcatagctt ttctacaaag
    11581 ccatttatca atctcatttt tattgaaacg gacagtctta ccaattttaa taacgggtag
    11641 ccccttttgt atccagccat ctaatgtatt gttagatatc cctagatatt tgcaagtttg
    11701 ccccttgtta aggtaaggac tatctaaacc taaatcttct ttgaaatgag aaatttcatt
    11761 ttgcaagaga ttagtaatca tcgactgtat ttcttgaact tgttctgttg gtagaataac
    11821 ttgcattt
//
LOCUS       DAVHIU010000053        11786 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVHIU010000053 DAVHIU010000000
VERSION     DAVHIU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.055"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..241
                     /locus_tag="VVN06_001237"
     CDS             <1..241
                     /locus_tag="VVN06_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5562783.1"
                     /translation="GTGFLISLLFECLQYILAIGITDITDLTLNTLGVCVGLLIYQIF
                     IRVFKSQTRKWINILGMLSLGFAYLVLLLLHLIGV"
     gene            266..1813
                     /gene="purH"
                     /locus_tag="VVN06_001238"
     CDS             266..1813
                     /gene="purH"
                     /locus_tag="VVN06_001238"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5562784.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            1935..3197
                     /gene="purD"
                     /locus_tag="VVN06_001239"
     CDS             1935..3197
                     /gene="purD"
                     /locus_tag="VVN06_001239"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5562785.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            3600..4088
                     /gene="purE"
                     /locus_tag="VVN06_001240"
     CDS             3600..4088
                     /gene="purE"
                     /locus_tag="VVN06_001240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5562786.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            4075..5166
                     /gene="purK"
                     /locus_tag="VVN06_001241"
     CDS             4075..5166
                     /gene="purK"
                     /locus_tag="VVN06_001241"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU5562787.1"
                     /translation="MSSSKTIGIIGGGQLGQMMATSAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            5176..5403
                     /locus_tag="VVN06_001242"
     CDS             5176..5403
                     /locus_tag="VVN06_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU5562788.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            5466..6764
                     /gene="purB"
                     /locus_tag="VVN06_001243"
     CDS             5466..6764
                     /gene="purB"
                     /locus_tag="VVN06_001243"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5562789.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(6819..10757)
                     /gene="strH"
                     /locus_tag="VVN06_001244"
     CDS             complement(6819..10757)
                     /gene="strH"
                     /locus_tag="VVN06_001244"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU5562790.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(11072..>11786)
                     /locus_tag="VVN06_001245"
     CDS             complement(11072..>11786)
                     /locus_tag="VVN06_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5562791.1"
                     /translation="AIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITV
                     VRALNDLAKDGYIVRQQGKGTFISRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKL
                     VYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFK
                     LDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSE
                     TYKRSDYYKIKFISCDRDH"
BASE COUNT         3138 a         2404 c         2730 g         3514 t
ORIGIN      
        1 cgggacaggt ttcttgatta gtttattgtt tgagtgctta cagtatattt tagcaatagg
       61 tataacagat ataacggatt tgactttaaa tacgctaggt gtctgtgtag gcttactgat
      121 ttatcaaatt tttataagag tgttcaaatc acagactaga aaatggatca atatcttagg
      181 tatgcttagc cttggttttg cttatcttgt tttactgtta ctgcatttaa ttggtgttta
      241 actaatgatt aaaaaggaga atataatgac taaacgcgtc ttaatcagcg tctcagacaa
      301 agcgggcatt gttgaatttg cccaagaact caaaaaactt ggttgggaga ttatctcaac
      361 aggtggaact aaggttgccc ttgataatgc tggggtggat accattgcta tcgatgatgt
      421 gactggtttc ccagaaatga tggacggtcg tgtgaagacc ctccacccaa atatccacgg
      481 agggcttctc gctcgtcgtg acttggatag ccacttggaa gcggctaagg acaacaagat
      541 tgagctcatt gaccttgtgg tggtcaacct ttacccattt aaggaaacta tccttaaacc
      601 agatgtgact tatgctgatg cagttgaaaa tatcgatatt ggtgggccgt ctatgcttcg
      661 ttcagcagcg aaaaatcatg ccagtgttac agttgtggta gatcctgctg actacgctgt
      721 ggttttggac gaattggcag caaacggcga aacctcttat gaaactcgcc aacgtttagc
      781 agccaaagta tttcgtcaca cagcggctta tgacgccttg attgcagaat acttcacagc
      841 tcaagtgggt gaaagcaagc ctgaaaaact cactttgact tatgacctca agcaaccaat
      901 gcgttacggt gagaatcctc aacaagacgc ggacttttac cagaaagctt tgcctacaga
      961 ttactctatt gcttcagcca aacagctcaa cgggaaagaa ttgtcattta ataatatccg
     1021 tgatgcagat gctgctatcc gtatcatccg tgacttcaaa gatagtccaa ccgttgtggc
     1081 tctcaaacac atgaatccat gtggaattgg tcaagctgat gacatcgaga ctgcttggga
     1141 ctacgcttat gagtctgacc cagtatctat ctttggtggg attgtcgtcc tcaaccgtga
     1201 ggtggatgct gcgacagctg agaagatgca cggcgttttc ctcgaaatca tcattgcacc
     1261 aagctatacg gatgaagcgc tagccatttt gatcaataaa aagaaaaact tgcgtatcct
     1321 tgccttgcca tttaatgctc aagaggctag cgaagtggaa gcagaataca caggtgtagt
     1381 cggtggactt ctcgtgcaaa atcaagacgt ggtcaaggaa agcccagctg actggcaagt
     1441 ggtgactaaa cgtcagccaa ctgagacaga agcgactgct cttgagttcg cttggaaggc
     1501 tatcaagtac gtcaaatcaa atggtattat cgtgaccaac gaccacatga cacttggtgt
     1561 tggtccaggt caaaccaacc gtgtggcttc tgttcgcctt gccattgacc aagccaaaga
     1621 tcgtctggac ggggcggtcc ttgcttcaga tgccttcttc ccatttgcgg ataacgtgga
     1681 agaaatcgcc aaagcaggaa ttaaggccat catccagccc ggtggctctg tccgtgacca
     1741 agaatccatc gaagcagcgg ataaatacgg cttgactatg gtctttacag gtgtgagaca
     1801 ttttagacat taagaagata aaagggaaga aaacagtttc tttccttttt tggcttaaaa
     1861 tgctaactga aacaagatta aaacgaactt ttttgatata atgttggtaa ataattcgca
     1921 aaagaggttg aggaatgaaa ctgcttgttg tcggttctgg tggtcgtgag catgcgattg
     1981 ctaaaaagtt acttgaatca aaagacgtgg aaaaagtctt tgtagctcct gggaatgatg
     2041 ggatgactct ggatggtttg gaattggtaa atatctctat ttccgaacat tctaaattga
     2101 ttgacttcgc aaagaccaat gatgttgctt ggacctttat cggtccagat gatgcccttg
     2161 ctgctggcat cgtggatgat tttaaccaag ctggacttaa ggcctttggt ccgactaggg
     2221 ctgcagcgga gctggagtgg tccaaggatt tcgccaagga aatcatggtc aaatacggcg
     2281 ttccgacagc aatatatggc acattttcag atttcgagga agccaaggcc tatatcgaaa
     2341 agcatggtgc gcctatcgta gtcaaggcgg atggcttggc acttgggaag ggtgtcgtcg
     2401 ttgctgagac ggttgagcaa gcggtcgaag ccgctcatga gatgcttttg gacaataaat
     2461 ttggtgactc aggtgcgcgc gtggttattg aggaattcct tgaaggagag gaattttcac
     2521 tctttgcctt tgtcaatggt gataagttct acatcatgcc aacggctcag gaccacaaac
     2581 gtgcctatga tggcgacaaa gggcctaaca cgggtggtat gggtgcctat gcgccagttc
     2641 cacacttacc acagagtgta gttgatacag cggttgacac cattgtcaag ccagttctag
     2701 aaggggtgat taaagaaggt cgtccttatc tgggagttct ttacgcaggg cttatcctga
     2761 cagctgatgg accgaaagtc attgagttca acgctcggtt cggagatcca gaaactcaga
     2821 ttatcttgcc tcgcttgacc tctgactttg ctcaaaatat cacagatatc ctggatagca
     2881 aggagccaaa tatcatgtgg acggacaagg gtgtgactct gggtgtggtt gtcgcatcca
     2941 agggctaccc gctagactat gaaaggggcg ttgagttgcc agccaagaca gaaggcgatg
     3001 tcatcaccta ctatgcaggg gctaagtttg cggaaaatag cagagcactg ctctcaaatg
     3061 gcggacgagt ttatatgctc gttaccacag cagataccgt caaagaagcc caagccagca
     3121 tctaccaaga actatcccaa caaaaaatag aaggactctt ctaccgaaca gatatcggaa
     3181 gcaaggcaat taagtaaaga tataagaata acgcgccgta gtcgccaaac acgataatgg
     3241 tcgtcgtggt gaaaagacca gaacagtgaa tgttctggtc agggggaaac ttggagacct
     3301 taggctcaaa gtttaggaat gaaaccgaag gtttgcttcc gcctccatca cctaagatca
     3361 ttatcaaaaa gaaaaataaa aattcacaaa atacgttaat gatcgtatgg tttgcgagcg
     3421 ttagcgagct aatatagaac aatcaccgcc gttgtgaaag aacgattgga tgataatcca
     3481 atcgttcagg gaaattggaa gaccttgggt ttccaattta ggcatgagac acctttggtg
     3541 gctgctgccg tccctcacaa gctaaggtga ttgttgaaaa agaggaaaaa ggagaagaaa
     3601 tgaaaccagt aatttccatc atcatgggct caaaatccga ctgggcaacc atgcaaaaaa
     3661 cagcagaagt cctagaccgc ttcggtgtag cctacgaaaa gaaagttgtt tccgcacacc
     3721 gtacaccaga cctcatgttc aaacatgcag aagaagcccg tagtcgtggc atcaagatca
     3781 tcatcgcagg tgctggtggc gcagcgcatt tgccaggcat ggtagctgcc aaaacaaccc
     3841 ttccagtcat tggtgtgcca gtcaagtctc gtgctcttag tggagtggat tcactctatt
     3901 ctatcgttca gatgccgggt ggggtgcctg ttgcgaccat ggctatcggt gaagctggag
     3961 cgactaacgc agctctcttt gccctccgtc tcctctctgt agaagataag tccattgcgg
     4021 atgcacttgc caactttgct gaagaacaag gaaaaatcgc agaggagtcg tcaaatgagc
     4081 tcatctaaaa caatcggaat tatcggtggc ggtcaactgg gtcagatgat ggccacttct
     4141 gctatctaca tgggccacaa ggttatcgcg ctggatcctg cggcggattg cccggcctct
     4201 cgcgtggcgg aaatcattgt ggcaccttat aacgatgtgg acgccctccg tcagttggca
     4261 gaccgttgcg atgtcctcac ttacgagttt gaaaatgtcg acgctgacgg tttggatgcc
     4321 gttatcaagg atggacaact ccctcaagga acagatctgc tccgcatttc gcaaaatcgt
     4381 atttttgaaa aggacttttt gtcaaacaag gctcaagtca ctgtggcacc ctacaaggtc
     4441 gtgacttcta gcctagactt ggcagatatc gacttgtcga aaaactatgt cctcaagact
     4501 gcgactggtg gctacgatgg tcatggacaa aaggttattc gttcagaagc agacttggaa
     4561 gcagcctatg cgctagcaga ctcagcagac tgcgtcttgg aagaatttgt caactttgac
     4621 cttgagattt ctgtcatcgt gtcaggaaat ggcaaggagg tgacgttttt cccagttcag
     4681 gaaaatatcc accgcaacaa tatcctgtct aagaccatcg taccagcccg catttctgaa
     4741 agtctagcag acaaggctaa agctatggca gtgcgaatcg cagaacaact caacttgtct
     4801 ggaactctct gtgtggaaat gtttgcgact gatgatgacg ttattgtcaa tgaaatcgcc
     4861 ccacgaccac ataactctgg gcactattct attgaagcct gtgatttctc tcagtttgac
     4921 acccatattc tgggtgttct cggagcacca ttaccagtca tcaaactcca tgcgccagcc
     4981 gttatgctta atgtcctcgg tcagcatgtc gaggctgctg agcaatatgt gacagaaaat
     5041 tcaagcgccc acctccacat gtatggtaaa atagaagcga agcacaaccg caagatggga
     5101 catgtgacct tgtttagtga tgtaccggat agtgtggatg agcttgggga agggattgat
     5161 ttttaggaca agtctatgat acaaattatc gttaatacat ttattgaaaa ggataagact
     5221 ggagcagttg ttgaagtgtt gtatgccagt gctgaccaag ataaggtaca agctaaatat
     5281 gaagaactag ctgcacaata ccccgaaaat tatttagcta tctatgatgt accgctggat
     5341 acggatttga atacactaga tcattacccg tctgtgttta ttgaaaaaga ggagtttgag
     5401 tagaaatctt ggtttaccta gatagcttat tcccaacagc ttaagaagaa aggaaaaatt
     5461 aacacatgat caaccgttac tctcgccctg agatggcgaa tatttggagt gaagaaaata
     5521 aataccgtgc ttggcttgag gtggaaatcc tctctgacga ggcatgggct gagttggggg
     5581 aaatccctaa ggaagatgtg gctttgattc gcaagaaggc ggactttgac atcgaccgta
     5641 ttttggaaat tgagcaggag acgcgccacg atgtggtggc tttcacgcgt gcggtttctg
     5701 agactcttgg tgaagagcgc aagtgggttc actatgggtt aacttctacc gacgtggtgg
     5761 atactgctta tggttacctc tacaagcagg ccaacgacat catccgtcgt gaccttgaaa
     5821 acttcactaa tatcatcgct gacaaggcca aggagcacaa gttcaccatc atgatggggc
     5881 gtactcatgg tgtgcacgct gagccgacaa cctttggtct taaattggca acttggtaca
     5941 gcgaaatgaa acgcaatatc gagcgtttcg agcatgcagc tgctggtgta gaagctggta
     6001 agatttctgg tgcggttggg aactttgcca atatcccacc atttgtagag gagtatgtct
     6061 gcgataaact tggcatccgt gcccaagaaa tctctacaca agtccttcct cgtgaccttc
     6121 acgctgagta ctttgcggtt cttgccagca ttgcgacttc aatcgaacgt atggcgactg
     6181 agattcgtgg tctacaaaaa tctgagcaac gcgaagtaga agagttcttt gctaaagggc
     6241 aaaaagggtc ttcagcaatg cctcacaaac gcaacccaat cggttctgaa aatatgactg
     6301 gtctggcgcg tgtcattcgt ggtcacatga ttacggctta tgaaaacgtc gctctctggc
     6361 acgaacgcga tatttctcac tcatcagctg agcgtatcat cacaccagat acgaccattt
     6421 tgattgacta catgctcaac cgttttggaa atatcgtcaa gaacttgaca gtcttcccag
     6481 aaaatatgat ccgaaacatg aactcgactt ttggtcttat ctttagccaa cgggctatgt
     6541 tgacattgat tgaaaaaggc atgacccgtg agcaagccta tgacttggtg caaccaaaaa
     6601 cagcctactc ttgggacaac caagtagact ttaaaccact tcttgaggca gattcagaag
     6661 taacatcacg tctcactcaa gaagaaatcg atgaaatctt caacccagtt tattacacca
     6721 aacgagtgga tgatatcttt gaacgtcttg gactaggtga ttaattaaaa aataaacagc
     6781 gagcttcaat ctcgctgttt attttttatc gaaaagactt agtcttcttt tcttttagtg
     6841 agtccatagg ctgctagtgt ggccatgagt cctgcgacta ctagtcctgc agaatcgtga
     6901 gttcctgttt caggaagttt tttctctatt accacaggag ctggatcttg aggaagagct
     6961 ttgctttcct cagcaggagc agttgatgga gctggttggc ttgggatttc tagttttggt
     7021 ttttcttcag caatagcggc ttgtccgttt tcatcgccta catgtgttac catagttccg
     7081 acttcgacta tttgagtaac ggcttcctgt gctacgacac tatttacaag tgttttcact
     7141 tccttaccat cggcagaagt gctcacagag tagaagttgc tacgatgtcc attgacgccc
     7201 ttagtaatga cttgtgtttt tcctttgagt aagagtggat tttcacgagt cactgtggta
     7261 aatggaattt cttctttttg gatatccagt ctaggtttta cctcagtagt tggtgcaaga
     7321 ccactttcat cacccttgtg agttacagga gcgccaactt caaccacttg gtttataact
     7381 tctttggtta cctggctatc aaggactgtt tctgttgttt ttccattttc agtgagtaca
     7441 gagatgtaat gagttcgctc acctttgact cctgctgtga taatattttc ctgaccagct
     7501 gggaggttag gattttcttt cttgataact tcaaatggaa tttcttcagt tcttgtgatg
     7561 agttctggtc tggtttcaac attggcagcc acttcatttt catccaggct tcctgaatga
     7621 gttgcagctg gtttgaggcc ttgaagagcg gcttttaggt tggctacaag tgtgtcaagc
     7681 tcagcttgtt tattacggtt gaggttgtaa tttagagctg ttttagctgc gtcaagggcc
     7741 tcaagacttt ctttactata tccttctaag tttgtaggaa ttttagctaa ttcttcgcgg
     7801 agagcattat aattagcacg gaagtagtct ttgttgtggt ctgcaaaggc agtcatgagt
     7861 tcaaagattt cctcttcctt gtattcagcg cttggtctat ctgcccagat tgaaagcata
     7921 cttccgactg ttggaagatc tacttcagga tatttggtag aagctagttg attgaatggt
     7981 gtttttccag tattctcaat agctttcttg aggaaaccac caccatcttc tggtttttga
     8041 ccaagaatgt agtaccagtc accgttggta ttcaagaatt tatagccttt gcttgctagg
     8101 tattgaggtg atgcgaggtt atatccccac cagcctttag accagtaaga aatcaagaca
     8161 tctttgtcaa actgaacatc gtccttgtct tcatagtaga agccatcgtt gaaggccatt
     8221 ggttgaagcc ctctttcttt ggccatagct gcgagggtgt tggcatattc ggcaaatttg
     8281 ccatagagtt gataccactt gaggtagtac cagccttggg cactagtcgc atcgttggcg
     8341 tattcgtcag taccaaagtt gaaaatcttt gttttacctg caaagaagtc catgtattta
     8401 ccgatgaggg cttttacaaa gttcatcgct tcttcgtttt tcaagtccat agttgttttt
     8461 gaaactttat caaagtgggc ttgaggattt ttaataccta atttttccat ggcaaccagc
     8521 atagcatcca tgtgacctgg actgttaata gctgggatga gaccgatgtc cttagattta
     8581 gcgtattcaa ttagctctgt tacttctgcc tgtgttagtg cagtaccgtt tggatcgtcg
     8641 tagtaagctt tagttccttc gataatagct tttttaacgt catcactagc ataggttttt
     8701 ccgttggcag taatggtcat atcatcgagt agaaagcgaa gtccgtcatt tcctagaagg
     8761 agatggacat cagaatatcc gagctcactg gccttgtcta cgatgcgttt gagctggttc
     8821 agagtaaagt atttgcgtcc agcatcgatt gagattacct tgtttttggc aagtttttca
     8881 acctcacgtt tagcttcttc ttctttttga gcttcaggcg tgagggtcaa gttgttgaca
     8941 gtttcttgaa gtttagcaat ggcttgatca atcgtatctt gttgggcacg gctaaggttg
     9001 ctatcgagag agcgaatagc tttttcagct tcttttacgg ccgcgacgct ttctgcagta
     9061 taacggttca ggtcttttgg tacctcgtta agtgcttgct ctgcagattc ataatcagct
     9121 gcgaagtatt cagcgttggc atttgcaaaa tgacgcatga gtttgaagag gtgtgatggt
     9181 gaataacgtg cagatggagt gtcagcccaa gcagctacca taccaccgat gattgggata
     9241 tcagctcctt ctgtttttgg tacagaagtg attggtgtgt ttttaatacc attgagcccc
     9301 tgatcgagat tgtaccagcc ttggccatca gcgtttcgtc caagaacgta gtaccaagca
     9361 tcattggtat taaggatttg gtgacctttt tcagctagta gtttagaaga agcgacatcg
     9421 tagcctcccc aaccaccagt ccacatagaa acgatgatgt ctttgtcaaa actaccaaag
     9481 cttgtgtcgc tattgtagta gataccgtcg ttaaaggcca ttggtttgag accgtgcgat
     9541 tttacaatac gagcgaggtc attggcgtag gcaataaatt tttcatagcc ttttacaggg
     9601 tagccttcgt ttggatagta tttatcagct tgaagcacac tccaaccttt agcatctgtc
     9661 gcatcattgg catattcatc aagtccgatg ttgaagattt cagtcttttt cgcgaaataa
     9721 gcagcatact tgtcgataag ggcttttgta aaagcgacag cttgttcgtt gtcaagatcg
     9781 acagtacggg ctgatttctt cccaaaatag ctaaagttag ggttttggat tcccaattct
     9841 ttcatggcat tgagaatcgc atccatgtgt ccaggactat ttactgtcgg aatgagaccg
     9901 atacctttat ctttggcata gttaatcaga tctgtcattt gactttctgt taagtgattg
     9961 ccgtttggat cgttgtaata atcatttgta cctttttcaa tggcgcgttt gacatcgtca
    10021 ctggcatagg tcttgccgtt agctgtgatg ctcatatcgt ccaacatgaa acggagtcca
    10081 tcatttccga ctaataggtg taaatcagtg tagccataat gtttcgcttt atcgatgatt
    10141 tccttgagct gttctggtga gaaatattta cgtccagcat caatagaaac aattttcttt
    10201 ttcgctagtt tttcatttac agttgcagca cgttcctttc ctgtctctgt ttccggtttg
    10261 tcagcctctg ctttcgcttc atctttttta gctggtttat ccttgtcagt cttgtctgta
    10321 tttgactctt tagaatcaac ctctttcgct tcttcctttt tagggctagc ttcttcttcc
    10381 tttttattag cagtttcttt ttcagcagaa gttggagtta ccacctctgc tttatcacta
    10441 ggagttgaac taacttcctc ttgtggtttt tcttctgttt ttggaagact agctacctta
    10501 tcagtagctg gagtttctgt ttctacagtt tttggagctt ctggttgaag ctctgcttta
    10561 ggtgtttcct cagtccgatt ttcggatgat tgaggggaat cagaaaccgt atggatggtc
    10621 ggttggtttt ctgtagtagt aggagtaact ccatcggctg caacagtctg tgcttggaag
    10681 gcaaatccaa ttagaacaga agctgctcct acagcgtatt tacgaataga aaaacgctgt
    10741 tgtttttcat gtttcattgc aaaacctcct gattgcattg ttatattgat agcgattata
    10801 taaatcaacg cctttatttt atttcttata ttaacgagag tcaagaggag atgacaaaaa
    10861 actataataa gtataaaaaa atataaaatt taaacttaag atttcagatt ggtcgaaaaa
    10921 atacatatat atatctagta taatttttgg ttctatttct ataaaatatt ccacaaatta
    10981 tagaatcttc caaaaatagg taagcgctac ctttttggtg tggtataata agcatagaaa
    11041 aagcccgagc gattaggctc aggctttctt cttagtgatc acggtcacat gagatgaatt
    11101 taatcttgta gtaatcagat cgtttgtaag tttcactgta ttctaaaact tggccagttg
    11161 attcgagttt ggtgatttta gtttgtagga cagtagggaa ttgttcatcg actccgagga
    11221 ctgaagccgc atgttctgga gttggaaaga ctatttcgtt gatttcttca aagtgttcat
    11281 cattcatgtg aatgtggtag tctaacttga aacgattata gatagaacta tagtattcaa
    11341 gatttggata atttgcgttg atatattgtt ctgggatgta ggatgtatgg tagatataaa
    11401 cgacaccgtt tgattcgcgg atacgttcaa tcttgtagta gaattgatcg ccgcgtagac
    11461 ccaatttttc caagtaaaca agcttgtttc cgcgttcaat tgaaagaaca gttaccttat
    11521 catctttagc attgaagagt tcaatatctg aaaactctac aagcttgtgt ttgcgtgcac
    11581 gtgaaatgaa ggttcctttt ccttgttggc ggacaatata gccatctttg gcaaggtcgt
    11641 ttaaggcgcg aacaactgtg atagagctga catcgtacat tgaaatgagt tctgcttcag
    11701 tgtaaaattt atctccactg ctaaactgcc cagagatgat tttatttttt aattcgtctt
    11761 ttatgtattg atatttggga attgcc
//
LOCUS       DAVHIU010000054        11751 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVHIU010000054 DAVHIU010000000
VERSION     DAVHIU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.056"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            450..1127
                     /locus_tag="VVN06_001246"
     CDS             450..1127
                     /locus_tag="VVN06_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU5562792.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            1114..1911
                     /locus_tag="VVN06_001247"
     CDS             1114..1911
                     /locus_tag="VVN06_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU5562793.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            1928..3031
                     /locus_tag="VVN06_001248"
     CDS             1928..3031
                     /locus_tag="VVN06_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5562794.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELILDPLNYPIENYVKRVKSE"
     gene            3332..4351
                     /gene="galE"
                     /locus_tag="VVN06_001249"
     CDS             3332..4351
                     /gene="galE"
                     /locus_tag="VVN06_001249"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5562795.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4353..5318
                     /locus_tag="VVN06_001250"
     CDS             4353..5318
                     /locus_tag="VVN06_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5562796.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFLYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(5369..5575)
                     /locus_tag="VVN06_001251"
     CDS             complement(5369..5575)
                     /locus_tag="VVN06_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5562797.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5627..6103
                     /locus_tag="VVN06_001252"
     CDS             5627..6103
                     /locus_tag="VVN06_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU5562798.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            6112..6783
                     /locus_tag="VVN06_001253"
     CDS             6112..6783
                     /locus_tag="VVN06_001253"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5562799.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLVVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            6914..7252
                     /locus_tag="VVN06_001254"
     CDS             6914..7252
                     /locus_tag="VVN06_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU5562800.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     ELKSEFVRKI"
     gene            7255..7884
                     /locus_tag="VVN06_001255"
     CDS             7255..7884
                     /locus_tag="VVN06_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU5562801.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            8124..9275
                     /locus_tag="VVN06_001256"
     CDS             8124..9275
                     /locus_tag="VVN06_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5562802.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            9587..10336
                     /gene="truA"
                     /locus_tag="VVN06_001257"
     CDS             9587..10336
                     /gene="truA"
                     /locus_tag="VVN06_001257"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5562803.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            10326..11099
                     /locus_tag="VVN06_001258"
     CDS             10326..11099
                     /locus_tag="VVN06_001258"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5562804.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNVGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            11077..11538
                     /locus_tag="VVN06_001259"
     CDS             11077..11538
                     /locus_tag="VVN06_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5562805.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNDWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         3342 a         2202 c         2693 g         3514 t
ORIGIN      
        1 attaaacaag taaaagaaca atacttaggg agacctgtga caatgatatc ttcctctgga
       61 agtagttcta attcaaacaa tgtttgttcc atgactgtat cctcctgtta tgataaattc
      121 cagaaagcat agagcaaatt cgtggttcgt ctgactaaat ttcttatctg aacaccacct
      181 tcaaatacaa attttagtca aacttttatt ttttctaact ttctaattat attctaccat
      241 gtgtttttat atgtcaagaa ttttctgaat aaactgttta ttttttattt tttgacattt
      301 ttgcaaattg tttaaaatct ttcaattgga ttcacttatt actttaggac attgaaactt
      361 ctttcgccag aggtgcttat attactggga agttgaagat tgaaatgtgg tataataaga
      421 ggtaatagag ttttggaaag tgagagaaga tgatttcaaa gagattagaa ttggtagctt
      481 cctttgtgtc acagggggct attttactag atgtgggaag tgaccatgct tatctgccta
      541 tcgagttggt tgagagaggc caaatcaaaa gcgctattgc aggtgaggtg gtggaaggtc
      601 cctatcagtc tgcggttaaa aatgttgagg ctcacggcct aaaggagaaa atccaagtcc
      661 gtttagccaa tggcttggca gcttttgaag agactgacca agtgtctgtc attaccattg
      721 ctggcatggg tggtcgtttg attgctagga ttttagaaga aggtttgggg aagttagcta
      781 atgtagagcg tttgatcctc cagcccaata atcgtgaaga cgacttgcgt atctggctac
      841 aggatcatgg attccagatt gtagcagaaa gcatcttaga agaagctgga aagttttatg
      901 agattttggt ggtggaagca ggacaaatga agctatcagc cagtgatgtt cgctttggtc
      961 ccttcttgtc caaagaagtc agtccagtat ttgtccaaaa atggcaaaaa gaagctgaga
     1021 agctagagtt cgccctcgga caaatcccag aaaaaaatct ggaagaacgt caagttctag
     1081 tagataagat tcaagctatc aaggaggtgc tccatgttag caagtgaagt gatccaagct
     1141 tatgaagcct tttgccctca ggaattttct atggagggag acagctgtgg tctgcaaatt
     1201 ggcactttag acaagggtat ccaaagggtc atggttgccc tcgatattcg tgaagagacg
     1261 gtggcagagg ccattgaaaa gggtgtggac ttgattatcg tcaagcacgc gcctatcttt
     1321 cgtcctatca aggacttgct tgccagccgt ccgcaaaatc agatttacat cgacctgatt
     1381 aagcatgata tcgcagttta tgtcagccat accaatattg atatcgttga aaatgggctc
     1441 aatgactggt tttgtcagat gctaggaatc gaggagacga cttatctgca ggaaacaggt
     1501 ccagaacgtg gaattggacg tattgggaat attcagcctc agacattttg ggaattggcc
     1561 caacaggtca agcaagtctt tgacctagat agccttcgaa tggtgcatta tcaagagaat
     1621 gatttgcaga agcctatttc aagagtagca atttgtggtg gaagcgggca gtctttctat
     1681 aaggatgctt tggcaaaggg ggcagatgtc tatatcactg gtgatatcta ctaccatact
     1741 gctcaggata tgctgtctga tggcttgtta gcattggatc caggtcacta tatcgaagtg
     1801 ctttttgtgg aaaaaatcgc agcactcctt actcaatgga aagagaaaaa aggctgggaa
     1861 ctagagattt tacctagtca agcatcgacc aatcctttcc gtcatatcta gttagaaagt
     1921 gaagacaatg aaaaaagttg ccattattgg agcaggaatt gtgggagcaa cagctgccta
     1981 ctacctctcg agagaaagtg acctagaggt gaccgttttt gaccatgagc aaggtcaagc
     2041 caccaaggcc gcagcaggaa ttatcagtcc ttggttttcc aaacgccgta ataaagcctg
     2101 gtacaagatg gcgcgcttgg gggctgactt ttatgtggat ttattagctg atttagagaa
     2161 atcaggacaa aaaatcgact tttaccagcg ttcgggagtc tttctcttga aaaaggatga
     2221 atccaagttg gaagaacttt atcaactggc cctccagcgc agagaagaat ctcccttgat
     2281 agggcaatta gccattctgg accaagcttc agctaatgaa ttattccctg gtttgcaggg
     2341 atttgaccgc ctgctctatg cttctggtgg agcgagagta gatggccaac tcttggtaac
     2401 tcgtctgcta gaagccagtc atgtcaagct ggtcaaagaa aaagtgactc tgacactctt
     2461 atcatcaggc taccagattg gcgaagaggt gtttgatcag gttattttgg caacgggagc
     2521 ttggttgggg gacatgttag agcctttagg ttatgaagtg gatgtccgtc ctcaaaaagg
     2581 acaactacgg gattatcagc ttgcccaaga catggaagat taccctgttg tcatgccaga
     2641 aggggagtgg gatttgattc cttttgcagg tgggaaattg tccttaggcg ctacccacga
     2701 aaatgacatg ggatttgatt tgacggtaga tgaaaccttg ctccaacaaa tggaggaggc
     2761 caccttgact cactatctga ttttggctga agctacttca aaatctgagc gtgtgggaat
     2821 ccgtgcctat accagtgatt tctctccctt ctttgggcag gtgccagaat tggcaggtgt
     2881 ctatgcagcc agtggactag gttcatcagg cctcacaact ggtcctatca ttggttacca
     2941 tctagcccaa ctgatccaag acaaggagtt gatcttggac cctctaaatt acccaattga
     3001 aaactatgtc aaacgagtaa aaagcgaata atactcaatg aaaatcaaag aacaaactag
     3061 gtaactagcc gcgggttact caaagcactg ctttgaggtt gtggatagaa ctgacgaagt
     3121 cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta accataccca
     3181 tggcaaggcg acgttgacgt ggtttgaatt ggattttcga agagtataag cattttactg
     3241 aaattttatc aggtagttta ggatggcaaa tgacattccc tgtcaaaaat ggtaaaataa
     3301 gaaaaaataa tctgagaatc gaggaaaaaa gatgcaagaa aagattttgg taactggtgg
     3361 tgccggtttt attggaaccc acactgttat tgaattgatc caagcaggcc atcaagttgt
     3421 tgtggtggat aatcttgtca atagcaatcg taagagttta gaagttgttg aaagaatcac
     3481 aggagttgag attcctttct atgaggcaga tattcgtgac acagctaccc tcagagatat
     3541 tttcaagcag gaagaaccga ctggtgtcat tcactttgct ggtttgaagg ctgttggcga
     3601 atcaacacgt atccctcttg cctactatga caacaatatc gctggaactg tcagcctttt
     3661 gaaagccatg gaagaaaaca actgtaaaaa catcatcttc agttcttctg cgacagttta
     3721 cggggatccg cacacagtgc ccatcttgga agatttccca ctttcagtga ccaacccata
     3781 cggtcgtact aagctcatgc tagaggaaat tttgactgat atttacaaag cagactcaga
     3841 atggaatgtt gtcttgcttc gttactttaa cccaatcgga gcccatgaga gtggtgattt
     3901 gggagaaaat ccaaacggta ttccaaacaa tctcttgcca tatgtgactc aagtagccgt
     3961 tggaaaatta gagcaagtgc aagtgtttgg agacgattac gatacggaag atggaacagg
     4021 tgttcgtgac tatatccacg ttgtcgattt ggctaagggt cacgttgcag ctttgaaaaa
     4081 aatccaaaaa ggttcaggac taaacgttta taaccttgga actggtaaag gttactcagt
     4141 tcttgaaatt atccaaaaca tggaaaaagc ggtgggacgt cctattcctt accgtatcgt
     4201 agaacgtcgc ccaggtgata tcgctgcctg ctactcagac ccagcaaaag ctaaagcaga
     4261 actcggttgg gaagcagaac tcgacatcac ccaaatgtgt gaagacgcat ggcgttggca
     4321 aagcaagcat ccaaatggat ttgaagacta agatgatgat ttcaatcatc gtcccttgtt
     4381 taaacgaaga ggaagtactt cctctttttt atcaggctct ggaagcttta cttccagatt
     4441 tggaaacaga aatcgagtat gtctttgtcg atgatggatc aagtgatggg accttggaac
     4501 tcttaaaggc ctatcgggag caaaatccgg cagtccatta tatttctttc tctcgaaatt
     4561 ttggcaaaga agcagccctt tatgcaggct tgcaatatgc gacaggagat ttggtggtgg
     4621 tgatggatgc agacctccaa gatcctccta gtatgttgtt tgagatgaaa aatgtactag
     4681 acaaaaatgt agacttggac tgcgttggga cacggagaac tagtcgggag ggagaaccct
     4741 tctttcgcag tttctgtgct gttctctttt atcgcctcat gcaaaaaatc agcccagtgg
     4801 ctctgccgtc gggtgtccgt gattttcgta tgatgagaag gtctgtagtc gatgccattt
     4861 taagcttgac cgagtccaat cgtttttcaa aggggctctt cgcctgggtg ggtttcaaaa
     4921 cctactattt ggactatcca aatgtcgaaa ggcaggctgg caagacaagt tggagtttta
     4981 ggcaactctt tttatactcc attgaaggga ttattaattt ttcagatttt cccttgatta
     5041 tagcctttgt agctggtctc ctatcttgtt ttctttctct gctgatgacc ttttttgttg
     5101 tggttcggac cctcattttg ggcaatccga catctggttg gacctctctg atggctgtta
     5161 ttctctttct tggaggcatt caactcttga ccattgggat tctcggcaag tatatcagta
     5221 agatttattt agagactaaa aaaagaccac tttatcttat caaagaaaaa agtgaccttc
     5281 ctgattttac agaaaaaaat aaagtgaaaa gactataatt ttacatggaa atgtgctaaa
     5341 ctagagggag tggattatcg ccattgattt aggcttcctg gtttccaatc agggcgcgag
     5401 ttgggcaatt tttaacagcc tctaagacat cctgactagg agaaatttct ttttccagtt
     5461 ggtcagggtc atcgtaaaaa cgcacgattc cattatcgtg gtaatcaaat aaatcagaat
     5521 aagtttggca aagcccacag gcgatacatc gttcaggtat aagtgtgatt ttcatgttca
     5581 tatttatatt gtaataagaa aagtaaaaaa aaacaaggag taaggtatgg caaaagaacc
     5641 gtggcaagaa gatatctatg atcaagaaga atcaagagca gagcgtcggc atcgaaacca
     5701 cggaggggct gataggatgg ctaatcgtat tttgacgatc ctagctagta ttttctttgt
     5761 aattgtggtg gtgatggtca tcgttctcat ctatctatca tcggggggga gtaatcgcac
     5821 agcagcctta aaagactttc atgattctga tgcaagtgta gtacaaatct catcttcaag
     5881 cagttctcag cctgagcaga gttcagagcc agaatctact tctagtagtt cagaagaagc
     5941 tgctaatcct gaaggaacga ttaaagttct cgcaggagaa ggggaagcag ctattgccgc
     6001 tcgtgcagga atctccattg ctcagttaga ggccttgaat cctgggcaca tggctacagg
     6061 atcttggttt gctaatccag gtgatgttat aaaaataaaa taggagccga tatgaaaaca
     6121 attcaaattg ctattgatgg tcctgcttcc agcggtaaga gtacggtcgc aaagattatt
     6181 gctaaggatt ttggattcac ctaccttgat acaggagcta tgtatcgtgc agcgacctat
     6241 atggctctta agaaccaatt agtagttgaa gaagtcgaag cccttctagc cttgttggac
     6301 cagcatccaa tcagctttgg acgttcagaa actggagacc agcttgtttt tgtaggagat
     6361 gtggatatta cccatcctat ccgtgaaaat gaagtgacca atcatgtttc tgctattgca
     6421 gcaattcctg aagtgcgtga gaaactggtt tctctccaac aagaaattgc ccagcaaggc
     6481 gggattgtca tggatggtcg cgatattgga actgttgtat tgccacaagc agaattgaaa
     6541 attttcctag tagcttctgt tgatgagaga gcagagcgtc gttacaagga aaatattgcc
     6601 aagggaattg aaacagacct tgaaacccta aaaaaggaaa ttgctgcgcg tgactacaag
     6661 gatagtcatc gtgagacttc tcctctcaaa caagcagagg atgctgtcta ccttgataca
     6721 actggtttga acattcaaga agtagttgaa aaaatcaaag cagaagctga aaaaagaatg
     6781 taaatgtaga aaagccgata gattgatatc ggtttttttc taaatttgtc aaaatgatga
     6841 ttttagggta taatagttgc tgttcgagaa attttgattt tcaaagaata gtgaatgatg
     6901 ataaggagaa accatgaaca atttaccaaa ttgtccaaaa tgtaactcag agtatgtcta
     6961 cgaagacggt gccctactgg tttgcccaga gtgtgctcat gagtggaatc ctgctgaagt
     7021 tgcagaagta gaagaaggtc ttgtcgctat cgatgccaac ggaaataaat tgactgatgg
     7081 tgatacagta actctcatca aggacttgaa agtaaaaggt gcgccaaaag atttgaaaca
     7141 agggacgcgc gtgaaaaata tccgcatcgt agaaggcgac cacaatatcg actgtaagat
     7201 tgatggtttt ggtgccatgg aacttaaatc agagtttgtg aggaagattt aagcatgtca
     7261 aaacactata aacttgtatt ttatagccgt atcttcttgt ttctagcggc ttttacggga
     7321 gtttatcttg aaattactaa gcatggtggt tttgggatgc ttctctatta cacggttctg
     7381 tccaacctct tggtaactat ttttaccctt tatcttctaa aggttatgag ccgtgtaggt
     7441 gaaaattggc aaaggccaag tctcttgcgc ttaaaaggtg gggtcaccat gagtatcatg
     7501 attacctgtg tgatttacca tttcctcttg gcgcccattg cgactaattt ctatacccta
     7561 gaaaatttcc tttgccacta tatcgttccc atctggtttt tagcggatac cctctttttt
     7621 gacaaacagg gtcaatacaa gatttgggat ccagcagtgt ggaccatttt accctttctg
     7681 tatatgatgt ttgctctttt taatggcttg gttctaaaac tcaatattcc aaatgctaag
     7741 gataatcctt tcccttactt ctttttgaat gtgaacaagg gttggaatgt tgtgtttaag
     7801 tggtgtctga ttatctttgt tgcctatatg gtagcaggat ttattttcta ctttatcaag
     7861 caaatcaaga gaaagtcatc ctaatactct tcgaaaatct cttcaaacca cgtcagtttc
     7921 catctgcaac ctcaaaacac tgttttgaac aacctgcggc tagcttccta gtttgctctt
     7981 tgattttcat tgagtataag atacaaggga ttcattttcg aatttaagaa ggagtctaca
     8041 aaccaatgag gcttcttctt ttcttgctta tttgataact atcaaaaaag tagaagatta
     8101 agagaaatat agaaagagat ttcatgaaac aatttttaga acgggccagc attttggctc
     8161 tctccctcgt tttgattacc tccttttcca tttcgagtgc cctaccagcc atgtttgact
     8221 attatcaggg ttattctaag gaacaaattg agctcttggt gagcttgcct tcctttggaa
     8281 tcatgatgat gttactgcta aatggtttct tagaaaaaat atttcctgag cgcttacaga
     8341 ttagtttggg cttgctgatt ttatcattga gcggtacagc tcccttctgg taccaagcct
     8401 atccctttgt ctttggaaca cggcttctct ttggtttggg tcttgggatg atcaatgcca
     8461 aggccatttc tattatcagt gaacgctacc aaggaaaaag gcgaattcag atgttagggc
     8521 tacgcgcttc tgcagaggtc gttggagctt ctctcattac cttggccgtc ggtcagttgt
     8581 tggcctttgg ttggacagct atctttctag cctatagtgc tggatttttg gtgctgcccc
     8641 tttatctgct ctttgtccct tatggaaaat caaagaaaga agtcaagaaa agagcgaagg
     8701 aagcaagtcg tttaactcga gaaatgaaag gcttgatttt taccttagct atcgaagcgg
     8761 cagttgtagt ttgtaccaat acagctatta ccatccgtat tccaagtttg atggtggaaa
     8821 gaggattggg ggatgcccag ttatctagtt ttgttcttag tatcatgcag ttgatcggga
     8881 ttgtggctgg ggtgagtttt tctttcttga tttctatctt taaagagaaa ctgctcctct
     8941 ggtctggtat tacctttggc ttggggcaaa tcgtgattgc cttgtcttca tccttgtggg
     9001 tggtagtagc aggaagtgtt ctggctggat ttgcctatag tgtagtcttg acgacggtct
     9061 ttcaacttgt ctctgaacga attccagcta aactcctcaa tcaagcaact tcatttgctg
     9121 tattaggctg tagtttcgga gcctttacga ccccattcgt tctaggtgca attggcttac
     9181 taactcacaa tgggatgttg gtctttagta tcttaggagg ttggttgatt gtaatctcta
     9241 tctttgtcat gtacctactt caaaagagag cttaggattg attcctaagt ttttcttttg
     9301 atactcaatg aaaatcaaag agcatagttg attgagtttg gaatagtacg ctgtagctgc
     9361 taaaatattt ctagaaatta atttgacttt cctaatagag ttgttcatat cttatttcaa
     9421 tctactatag aaagctagcc gcaggttgct caaaatagtg ttttgaggtt gcagatggaa
     9481 actgacgtgg tttgaagaga ttttcaagag tataagattt ttatacccat acaatatttg
     9541 ttttaggtcc ttgtttacag actaatttct tgataaaata gaggttatga cgagatataa
     9601 agcaactatt tcctatgatg gttatgcctt tgctggcttt cagcgccagc ctcatgcgcg
     9661 tagcgttcag gaagaaattg aaaaaacctt gaccagatta aataaagggc aaaccattac
     9721 tgttcacggt gctggtagga cagatagtgg ggttcatgcc ctgggacagg tcattcattt
     9781 tgacctgcct tatcagatgg atgaggagaa actccgtttt gccttggata cccagtctcc
     9841 tgaagatatt gatgtgattt cgattgagct tgtggcagct gattttcatt gccgttatgc
     9901 caagcatagc aagacctatg agtttattgt ggatagagga cgtcccaaaa atccgatgcg
     9961 ccgtcactat gccacccact ttccctaccc actcgatgtg gaacgaatgc agattgcaat
    10021 caaaaagcta gagggaaccc atgattttac cggttttaca gcctctggga ctagtgtaga
    10081 ggataaggtt cgcaccatca cagaagctag tttaatagtc gatgagacag gacaattttt
    10141 gacctttacc ttttccggaa atggtttctt gtataaacag attcgcaata tggtggggac
    10201 tcttctcaaa atcggtaaca accgcatgcc agtagagcag attgatctca tcttggagaa
    10261 gaaggacagg caacttgcag gtcccactgc agcaccaaat ggtttgtatt taaaggagat
    10321 tcgttatgaa gaataatcgt attttagcac tttctggaaa tgatattttt agtggtggtg
    10381 gactgtcagc tgatttggct acctatacct tgaacggctt gcatgggttt gtagcagtga
    10441 cttgtttgac agccttgaca gaaaaaggat ttgaagtctt tccaactgat gataccattt
    10501 ttcaacatga attagatagc ttgcgtgatg tggaatttgg gggaattaag attggtcttc
    10561 tccctactgt cagtgtggct gagaaggcct tggactttat caaacaacgc ccaggagtac
    10621 ctgtggtgtt ggatcctgtc ttggtctgca aggaaacgca tgatgtagct gtcagtgagc
    10681 tctgccaaga gttgattcgc tttttccctt atgtcagtgt gattacgcct aatctcccag
    10741 aagcagaatt attatccggt caggaaatta aaaccttgga agacatgaaa actgcagcgc
    10801 agaaattgca tgatttagga gcgccagcag tcattatcaa gggaggcaat cgtcttagtc
    10861 aggacaaggc tgtggatgtc ttttatgatg gacagacctt tactatccta gaaaatccag
    10921 ttatccaagg ccaaaatgtt ggtgcaggtt gtacctttgc ctctagcatt gccagtcact
    10981 tgattaaagg tgataaactt ttgccagcag tagaaagctc taaggctttc gtttatcgtg
    11041 ctattgcaca agcagatcag tatggagtaa gacaatatga agcaaacaaa aacaactaaa
    11101 atcgcccttg tatccctatt aaccgccctt tctgtggttc taggttattt cttaaaaatc
    11161 ccaacaccta caggaattct aactctttta gatgctggtg tcttctttgc ggccttttac
    11221 tttggtagtc gtgaaggagc ggtagtcgga ggactagcaa gtttcttgat tgacctctta
    11281 tcaggctacc ctcagtggat gttctttagc ttggtcaacc atggcttgca gggatttttc
    11341 gcaggattta aaggaaaaag tcagtggtta ggccttattt tagcaactat tgccatggta
    11401 ggaggctacg ccttgggttc tactttgatg aatgactggg cagcagccct cccagaaatt
    11461 ctaccaaatt ttatgcaaaa tatggtaggg atgattgtag gatttattct tagtcaaagt
    11521 atcaagaaga ttaagtaata ctcttcgaaa atctcttcaa accacgtcag cttccatctg
    11581 caacctcaaa acactgtttt gagcagcctg aggctagctt tctagtttgc actttgattt
    11641 tcattgagta taaagaggtt gagtaaaaag tatttcaaat aagaaaaacg catagtatag
    11701 gatgttgaaa tgatgaactg ccccccaaaa gttagacaga aaaaatctaa c
//
LOCUS       DAVHIU010000055        11618 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVHIU010000055 DAVHIU010000000
VERSION     DAVHIU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.057"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(197..367)
                     /locus_tag="VVN06_001260"
                     /pseudo
     CDS             complement(197..367)
                     /locus_tag="VVN06_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(510..1109)
                     /locus_tag="VVN06_001261"
     CDS             complement(510..1109)
                     /locus_tag="VVN06_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562806.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(1246..2130)
                     /locus_tag="VVN06_001262"
     CDS             complement(1246..2130)
                     /locus_tag="VVN06_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU5562807.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(2134..2520)
                     /locus_tag="VVN06_001263"
     CDS             complement(2134..2520)
                     /locus_tag="VVN06_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU5562808.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2513..3856)
                     /locus_tag="VVN06_001264"
     CDS             complement(2513..3856)
                     /locus_tag="VVN06_001264"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5562809.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3943..4827)
                     /locus_tag="VVN06_001265"
     CDS             complement(3943..4827)
                     /locus_tag="VVN06_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562810.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4839..5456)
                     /gene="cysE"
                     /locus_tag="VVN06_001266"
     CDS             complement(4839..5456)
                     /gene="cysE"
                     /locus_tag="VVN06_001266"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU5562811.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHSFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHAVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5472..7685)
                     /gene="pnp"
                     /locus_tag="VVN06_001267"
     CDS             complement(5472..7685)
                     /gene="pnp"
                     /locus_tag="VVN06_001267"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5562812.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(8370..9236)
                     /gene="metF"
                     /locus_tag="VVN06_001268"
     CDS             complement(8370..9236)
                     /gene="metF"
                     /locus_tag="VVN06_001268"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU5562813.1"
                     /translation="MSCQTPSLSFEVFPPNPAVGNDNIILALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNEHFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(9300..11549)
                     /gene="metE"
                     /locus_tag="VVN06_001269"
     CDS             complement(9300..11549)
                     /gene="metE"
                     /locus_tag="VVN06_001269"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU5562814.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
BASE COUNT         3260 a         2618 c         2281 g         3459 t
ORIGIN      
        1 gttcaaggta ataatccaaa cacgaaacca gtccacgttt ttcaaggact ggttttgata
       61 tagcacgttt aagtactgac ttctgagcta ctatagtaga ttgaaactag aatagtacac
      121 ctctacttct aaaatattgt tagaaatcga tttgacttcc ctgattgatt cgtcctattc
      181 ttatttcatt ttaatataat tgatagtggt cgccccagcc agatacctta tctgctatcc
      241 atttaggaac ccctaactta agcaatcccc ataatcgtct cgatttcttc ttccattgct
      301 tccagataat cactcgtagg cgagtacgca agcgctcatc tatgctagtg actatacttt
      361 tcatatttat aattcattcc tttcgtttca ctcaaggcac aacacagaat gaaaaagtgt
      421 tgtgatcttt attttgtttt ataataatag tgagaaagcc tatcactact acaaatcacg
      481 gggaggtgaa taagtgagtg gtacagccac tacctcgcat attttgtcac atcatttaac
      541 ggtacataat aagttgtacc atctgaataa gttgctacaa tatcatttgc atgctctcct
      601 tcacctttag caaaggttgg agctcctgct ggatgatttt tatttgcctc tttcaatttt
      661 tcaataatgg catttttttc tgtatctttt atattatcag gatttttcac taagattttg
      721 tctggatatg tcggtttagc agaaacaatt tttactgtta cttctttttt attcgaagca
      781 cttgtccagt ttccagcatt atctttagca tttaatttta cagtaattcc tgaactagga
      841 acttcagtag caggttgatt atcaacatta ttcaacttta atttcaaaag agctgttgca
      901 tcagacgttt tatcaatcgt tatatataat gatgaattgt tattataacc agttccttca
      961 tatttagctg tttgtgagct acttgaaaca gaactgaaat tatacccact acctccctga
     1021 ttatcttcaa tgcttacgtc taaatgaact tccccactat tatttggctt agcaacaact
     1081 gttatagtaa aataacataa aatttgcata aatagattag ggaaatcaaa gcagcttcta
     1141 ggaatgtttt agcagtcaca gtgtactttc ccagcatcaa gccactataa ctctgcacat
     1201 aaaaatggag aagatggcaa tcctcttctc caaatattaa cttctttaca aaccaactat
     1261 agttgacaaa gaacctaaaa tcaattgata acacaaggtc aggtcggtca actctttcaa
     1321 ctgaagccct gtcaactctt cccatttatc aatcttgtat tggagagaat tgcggtgcag
     1381 atagagttgc tgggctgttt tagtgagaac agcactattt tcccaaagag agagaatgat
     1441 ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga cattctttaa ttggcttcaa
     1501 gtccacgagt ctttctccca tactccaaag atagagctga gaaaaagtat gaacaccttg
     1561 gtgaccctga cgccaccatg tcttgaacaa atcccgctca gctttgatta agtctgatag
     1621 ggcttgatgt cctgtctgag accaaacctg acccaacatg atagaaagac gaagtccaaa
     1681 atcatactca accgcttcaa tcgtatcact taaaatatct cttacagaag tgtatttgtc
     1741 ttgttgaagc acgaaaacat aatcctgagc tccgacctgt agcactgtct gacaattcgg
     1801 aaaaagagtc cgcatcatat ctagccaaga agtcagattt tcctgctgaa aataagaaag
     1861 atggcaataa accaactgaa tctttttaaa aacttgcggt gcctgtccct tgccttcaac
     1921 cagataggaa taccaagggt ttagcgaacg agcctgctcc tgctgggtca aaagggcaac
     1981 caactgcttt tcacgctcgc tgagcccagc ttcctccagc aaaatccact gctgagaagc
     2041 taaagggagc gtgagatagc cctctttctc tactggttgg tctgaaatcc gagcctcagg
     2101 aaaccagtct tgtagttctt tttccctcat gttctagccc tccacttttt ggatgcacca
     2161 tgaaaccaaa ctctcaagac gttccagatt ctcagtcata tggagatagc ccataaccgc
     2221 ttcaaatccc gtggacatac gataagtcac gacatctgca tttttagcct ttgtgtggct
     2281 attggtattg cggccacgtt tgtagatttc ttcttctttt tccgttagga cctgctcctc
     2341 caacatgaga gcaatcaggc gagcctgagc cttggctgac acatacttgg ttgcttcttg
     2401 atggagttta ttgggtttgg tcataccttt gaggatgagg tgacggcgaa tatacataga
     2461 atacaccgca tccccctcaa aggctagcgc aatcccgtta atgagattga catcaatcac
     2521 gtgtccacct cactccatcc ttggtatcaa ggagcttaat tccttgagta accaattggt
     2581 cacggatttg gtctgctgtc gcaaagtcac gattggcacg cgcctcttgg cgtttttgaa
     2641 tcaagtcttc aatctctgca tccaaaactt cctcaacaaa gacaattcca aaaatttcta
     2701 acatatctgc aagagcttgc ttgacacttg catcatagtt ccctgagttg atccatttgg
     2761 ccatttcaaa gacaactgtg ataccgttgg cagcattaaa atcttcatcc atagctgcta
     2821 caaacttatc tttaaagttt tgtaactctt gggcatccac gtttcctgta aatggttgtt
     2881 cgtaagtatt cttcagatac ttgagattgg tctcggcatc gcgaactgcc ttttccgtga
     2941 agttgatagg cttacggtag tgctgggtcg caaagaagaa acgaagtact tgcccatcaa
     3001 gagttttaag ggcatcgtgt accgtaatga agttacccaa ggacttagac attttgacat
     3061 tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc aaaagccttg cctgttttag
     3121 cttcagattg ggcaatttca ttggtgtggt gtggaaactc taggtcagct ccaccaccgt
     3181 ggatatcaat ggtatcacct aaaatctctg tcgacatgac tgaacactca atatgccaac
     3241 ccggacgtcc aggtccccaa ggactatccc aagaaatctc acctggtttg gaagatttcc
     3301 atagagcaaa gtctacagga ttttccttac gagccgtttc ttcatcggta cgacctgaag
     3361 cacctagctc caaatcttcc aaggttttat tagccaattt agcatagttg tgggattttt
     3421 ctacacggaa atagacatcc ccttgactct cataggcaaa gcctttctcg atcaagtctt
     3481 ccacaaaacg gatgatgtct gccataaact ccactacacg cggatggcga gtcgcaggtt
     3541 tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat gtacttatcc gcaacctcct
     3601 gaggcgtgat gccttcttcc ctggcacggt tgataatctt atcatccaca tctgtaaaat
     3661 tggaaatata ggcaacctta tacccacggt actcaaaata gcgacgaatc gtatcaaaag
     3721 ctaccgtcga acgggcgttt cccacgtgga tatagttgta caccgttggc ccacaaacat
     3781 acatcttgat cttgccgtcc tcaatcggga caaattctcg caaatcacga gacatggtgt
     3841 catagatttt aatcataaat cataatcagg aaagctaaaa tccaagaact gttagtttca
     3901 tcactaaact aagagctcaa gtaaatttca gtccgaatgt ctctacactt cggaatccct
     3961 tgctcctttc tcattcagat aaaccacctg tgtctgtttg acaaagccaa ttttttcata
     4021 caaacgtttg gcacctacat tgctatcttc cactgcaatc tgaaattcct tatcattttg
     4081 ctcaattagt tggttgacaa gggattttgc taagtagctt ccatagcctt tcccacgttc
     4141 aggttctgat attgctaaac cgtagaagta attcgtatcg ctcgataaat caaccgtgca
     4201 agtcccaata acctgaccag cttttaataa aatatatagt cggctttctg gatctttcag
     4261 agcttcagcg acatatctat ccacaacttc tctcgattca tgttcctctg aaaatgcctg
     4321 aaattttaat tgactaattt gatcctgata cgaactatct gctaacaaaa tttcaagatg
     4381 ggaaacattt gctaacggat aaggtcttct atccttacct aaccaagttt ctgtctcttc
     4441 atcctcgatt agtccccagt tactggcaaa gtcaggatga ttctctaaaa aaatacgttc
     4501 tgtctgaaaa gtgactgacc gaatagggaa agaagctgtt tctctctcaa aactagtaaa
     4561 caatgcacgc gcaatcccct gacggcgatg acctggatga accagtatcg tcacttctac
     4621 atcttggtca tctgcataga cagttaataa accaacaagt ttgccttttt cataataaag
     4681 gaaaaaggcg ggcatgtttg ggtcaaaatt aagcatgtta gagagatagg gatcgcgata
     4741 ggtaccgtca tagttttggc aacagttaat tacttttttc gcctcagata gctcctcttg
     4801 gcttaacttg tttcttgctt gaatcatata ggtatcctct acaaaccaga cgatctgtga
     4861 ctggcatctt tagcctgctc gagtttattg acataatact ctcgtttttc ttcgactgcg
     4921 tgaataacag gctcatcttt cttaccatga agacggacaa tcttggccgg aataccgaca
     4981 accgtcacat cactaggtac atctgctacg acaactgctg cagcaccgac cttggcattt
     5041 tcaccaattt ccacaggtcc gataacttgg gcatgggctg atatgagggc tccctttcgt
     5101 acagtcggat ggcgtttgcc acagtctttc cctgttcccc caagagtcac tccgtgatag
     5161 agaagaacgc ctttttcaac aatcgctgtc tctccaatca ccagaccaga accatggtca
     5221 ataaagacac ctgaatcaat ctgggctcct ggatgaatct caatctgagt ccaaaagcgc
     5281 caaaactgac tgtgcatacg agctaagagt ttgaagctgt gcttccagag aaaatgcgag
     5341 agacggtggg ccgccaaggc cttgacacct ggataagtca gcaaaacctc caaagtggtg
     5401 cgggccgctg gatcattttc ttttacgata tcaatggttt cgcgccacca ccccatacat
     5461 ttctcctttt cttattctga atcttttggt gtttctgtaa attctttctt aggtttgtga
     5521 tccttgtgat gacgtgggcg gtgagggcgc tcagactttt cacctttttc atcatgctca
     5581 ggttttggcg gacgaggtag aagagccttc atagaagcat cgatacggcc tttttcatca
     5641 atcttgataa ccttaacatc aacttcatcc ccgatttcta ccaaatcctc tacacgattg
     5701 gtacgagtcc aagccatctc agagatatga acaagggcat ctgtcttatc aaagaggtta
     5761 acaaaggcac caaatttctc gatacgaacg actttagcac ggtaaacttc atccactttg
     5821 gcttcacgaa ccaaaccagc aataatttct ttggcacggt taatagcatc ttggtcacta
     5881 gagtagatag acacatttcc ttcttcgtct atatcaatct taacacctgt ttcagcaata
     5941 atcttgtcga tggtttctcc acccttaccg atgacaatct taatcttgtc cacatcaatc
     6001 ttgatcgtat caattttcgg agcagttgga gccaattctg gacgaacttc tggaatggtt
     6061 gcttcaatga catcaaggat ttcaaaacgc gctttcttgg cttgagcaag agcctccgtc
     6121 aagatttctg cagtaatccc ttgaatcttg atatccattt gaagggctgt aatcccatca
     6181 cgagtacctg caaccttgaa gtccatatct ccaaagtgat cttccaaacc ttggatatct
     6241 gtcaatactg tgtagttatt tccatctgag ataagtccca tagcaatacc agctactggc
     6301 gccttgattg gcacaccacc agccataagg gcaagagttc ccgcacagat agaagcttga
     6361 gatgaagaac cgtttgattc caaaacttct gctactagac ggatagcgta tgggaattct
     6421 tccaagcttg gcaagacttg agcaagagca cgctcaccaa gagcaccgtg accgatttca
     6481 cgacgacctg gcgcaccgta acgacctgtt tcccctacag aatattgagg gaagttatag
     6541 tggtgcataa agcgtttctt gtactctgga tccaaaccat caatgatttg agtttctccc
     6601 atcggagcca aggtcaagac tgaaagagct tgagtttgcc cacgagtaaa gagacctgaa
     6661 ccatgtacac gaggaaggaa gtcaacaacc gcatccaaag gacggatttc atcgacctta
     6721 cgaccatcag gacgcacctt gtcttctgta attaaacgtc gcacttctgc gtgttccatt
     6781 tgttccaaga tttcagccac atcacgcata atacggtcaa attcttcgtg gtccgcatat
     6841 ttttcttcgt aaacggcagt cacttggtct ttcactgctt gagttgcagc ttcacgggcc
     6901 aatttctctt ctacttgaac tgccttttgg aggtcactgt tgtaggctgc aatgatttca
     6961 gcttgcaatt cagcatccac gtgaagcaat tccacttctg ctttttcttt accgacagca
     7021 gcaacgattt cttcttggaa ggcaatcaat tctttgacag cttcgtgccc tttaagaagc
     7081 gcttccaaca tgatttcttc tgacaattct ttggcaccag actctaccat gttgatagcg
     7141 tgcttggttc cagctactgt caattcaaga agagattgct ctgcttgttc ttgacttggg
     7201 ttgatgatga tttggccatc tacatatccc acttgtaccc cagcaattgg tccgtcaaat
     7261 ggaatatctg aaatagacag tgccaaagat gaaccaaaca tagcagccat tggtgcagat
     7321 gcattttcat cataagaaag cactgtattg atgacttgga cttcattacg gaaaccttcc
     7381 gcaaacatag gacgaatcgg acggtcaatc aaacgcgctg tcaaggtcgc atctgttgaa
     7441 ggacgtcctt cacgtttcat aaagccacca ggaaacttcc cagccgcata cattttttct
     7501 tcgtagttga cttggagtgg gaagaaatcc ccagttgcca ttttcttaga cataacggca
     7561 gcagtcaaga cagttgactc accgtaacgt acgacaacag atccatttgc ttgcttagca
     7621 acctgaccag tctctacaat taactcacga cccgcaaaag tcgtttgaaa cacttgtttt
     7681 gccattttaa tcccctttgg attgatgaaa ttatacgcct tgcctacaaa gatcaagata
     7741 ccaaggacgt caaaagcaaa gtaaaaatag gaaactgacg aagtcttcga tgaagacaag
     7801 acagtttatc ttttttacac agcttttcgg ccgtgttcaa ttacacaaga tattttggac
     7861 ggttcggctt gccgaacatt tctgtagaaa aataggaagg tgacttcgca ctcgacgagt
     7921 gctaggaagc ttatcttttt tcctaagaaa tgagaccaaa attcaagtca tcaagatacc
     7981 aagccgtcaa gcaactcaaa ggaagatagg aaatcgaacg acggagcgac tgctcctagg
     8041 gagatttatc tttttccaca gagttgtagg caagttcagt tttcaagata catcattaga
     8101 aaggtttaat actaaagtat ctaaagcttt cacgctaatc gctatcgggc gattagctaa
     8161 atgctttact aactctctcg tcaaataaca tcgatttgac tcactcgtgt cgttaaatct
     8221 tacagtttaa atgcattgta ttatttaata ccttcatctt tgtatcaagt acgtacagaa
     8281 tttattttat catatttttc ttaaaaagtg aggtctttac cattaaaaag gaaccattcc
     8341 cctcacctga gaagaatggt ttgcttttat tatcctagag actggtgatt aaacaaggca
     8401 tgggttgctt gatggatgta ttttgctgta tcagcattat tcatcgtata gagatgcaca
     8461 ccggcaacat cctgagttac caagtctacg atttggtcca ctgcataggc aagtcctgct
     8521 gctctgagcg actcagggtc atgctcatac ttgtctaaga tggctttaaa tttgcgtgga
     8581 agatggatat tctcacaagt cttcaagagt cggagagcct gatttcgatt cagaattggc
     8641 ataattcctg catgaatggg aacatcaatc ccagccaaga tgcacttgtc ctgaaaatca
     8701 tagaagtgct cattgtcaaa gaagagctga gttacgaggc tcgaacagcc tgcatccact
     8761 ttcttcttaa gattttgaat atctgaaatc tgatttggcg aatctggatg cccttctgga
     8821 tagcaagctc caataatatc aaagtgaggg gcttgttcct tgataagctc aatcaagtcg
     8881 gttgcatagc ggaaatcctt ttgtggttcc acgtctggaa taatatcccc acgaagagcc
     8941 aagattttct gcaccccaac tttgtccaag tcagcaatag tttcagcaac cttgtcctta
     9001 gttagataaa tagctggcaa gtgggcaatg gtcggaatcg ccaagtcatt ttggataaag
     9061 tcagccaaac gaaccgtcgt ttccttgata ttaaatttat tattgctggc agttacactg
     9121 ataaaatggg gagccaactc ctgcatatcc tgcaaggcta aaataatgtt atcattaccc
     9181 acggctgggt ttggagggaa cacttcaaat gagagtgacg gtgtttggca tgacatatgt
     9241 aataaccttt tctagttgat ttctttttga acaaccactg tatggagaga aatccaatct
     9301 tacaatttct cacgcgcagc tttagctgct tcaacaaggc ggatcaagct ttcttttgtt
     9361 tctgggatac cacgtgtttt caaaccacag tcagggttga tccaaacttt cttgcttggc
     9421 actttagcaa ggatggcttc gattgtgttg tcgatttcgc cttcatttgg tacacgaggt
     9481 gagtggatat cgtaaacccc aggtcccact tctgtttgga agtttttcgc tttgagttcg
     9541 tccaagattt caaggtttga acggctggct tcaaaggaaa taacgtctgc atccatgtta
     9601 tcgatagctg ggatgatatc tgtaaattct gagtaacaca tgtgagtgtg gatttgtgtg
     9661 tctgacgcta ctgttgagtg taccaagcgg aaggcaggaa ttgcccagtc aaggtaatct
     9721 tcgtaccagt cactacgacg gagtggcaat ttttcacgaa gagcagcctc atcaatttgg
     9781 atgattttca caccagcagc ttcaaggtca agcacttcat ccttgatagc aagggcgatt
     9841 tgaagagtag aatccttgat agagatgtct tcacgtggga atgaccagtt gaggatggta
     9901 acaggtccag tcaacatacc tttaacaggt ttgtttgtac gactttgtgc atagctagac
     9961 catttaacag tgatagggtt aagacgagtg acatcacccc agatgattgg tggttttacc
    10021 ccacgcatac cgtatgattg tacccatcca tttttagaga agaggtatcc tgacaagttt
    10081 tgaccgaagt actcaaccat gtcattacgc tcaaattcac cgtgaacaag gacatcaaag
    10141 tcaatatctt cttgccactt gatccattcg tcaatcgttt cagcaaggaa agcgtcgtac
    10201 tctttttgag acaattcacc tttacggtaa gccaaacgtt tggcacgaac ttcttttgtt
    10261 tgagggaatg aaccaatcgt tgttgttgga agagctggaa gtttaaaagc ttcttcttgg
    10321 atagcttcac gttctgcaaa ggctggcaaa cgagtgtagt ctgcgtctgt caagccagcg
    10381 atacgcgcac gaagttcagc attttcacca acacgctcag tcgcaaagag ttctttgttg
    10441 gctgcaagag cttctgaacc ttgaccattg cggatagcat ccaagtcacg gatttcatcc
    10501 aatttttcaa ctgcaaaggc aaagtggttc aagagtgctg gttcaaattc ttcattagca
    10561 gttgtaaatg gcacatgaag aagtgagcaa gagcttgtca aaacgatgtt ttcagctggg
    10621 atttgctcaa gaacagccaa gctcttttcg tagttgttac gccagatgtt tttaccattg
    10681 acaatacctg catagagagt cttgtcagct gggaagccac ctttaacgag ttcaagagtt
    10741 ttcttacctt caacaaagtc aagaccgata gcatctactg gtaagtttac aaggtcagcg
    10801 tatacgtcac gaacatcacc gaaataagtt tgaagcaaga cttcaagacc ttttttgtca
    10861 gccaagagtt tgttgtaaag gttcaagaag agagcttttt cttcagctgt caagtctttt
    10921 acaagagccg cttcatccaa ttggatgcga gtcgcaccaa gttcagccaa tttagcaaaa
    10981 acttcttggt aagcaactac taagctatct acgaagtcgt ctgctttcac gccttcttca
    11041 aagtctgaca attgaaggaa agtgaaggga cctacaagaa caggacgagt gttcaatcca
    11101 agttcttttg cttcttggaa ctcatcgaaa atcttgtgac cagccaattt tacttgagtg
    11161 tctttttcaa atttaggaac gatgtagtgg tagttagtgt tgaaccattt cttcattgga
    11221 agggcgcgaa cgtccccttt ttctccctgg taaccacgtc ccaaagcgaa gtagcgctca
    11281 aggtcagaca agtccaagtt ttgaacggat gcaggcacca cgttgaaaag gaaagccgca
    11341 tctaggaagt tatcatagtg agaaaagtca tttgatggaa tttcagtgat gcctttttct
    11401 ttgacaatgt tccagtgttt agcacgcaag tcttttgctg ctgctaaaag ttcttcttct
    11461 gagatttctt ttctaaagta tttttcagtt gtaaatttta attcacggaa ttcgcccaaa
    11521 cgagggaaac cgatgattgt agttgacatg atgtgtcctc caaaatttgt tgttgaaact
    11581 atcttaacag aaaagaaagc gtctgtataa ttgtaaaa
//
LOCUS       DAVHIU010000056        11527 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVHIU010000056 DAVHIU010000000
VERSION     DAVHIU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.058"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            109..762
                     /locus_tag="VVN06_001270"
     CDS             109..762
                     /locus_tag="VVN06_001270"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5562815.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            772..1236
                     /locus_tag="VVN06_001271"
     CDS             772..1236
                     /locus_tag="VVN06_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU5562816.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTADLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1249..2517
                     /locus_tag="VVN06_001272"
     CDS             1249..2517
                     /locus_tag="VVN06_001272"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5562817.1"
                     /translation="MLLIKNGHVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2531..2791
                     /locus_tag="VVN06_001273"
     CDS             2531..2791
                     /locus_tag="VVN06_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562818.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2802..4163
                     /gene="pdrM"
                     /locus_tag="VVN06_001274"
     CDS             2802..4163
                     /gene="pdrM"
                     /locus_tag="VVN06_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU5562819.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAVVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4317..5285
                     /locus_tag="VVN06_001275"
     CDS             4317..5285
                     /locus_tag="VVN06_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5562820.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5301..6293
                     /locus_tag="VVN06_001276"
     CDS             5301..6293
                     /locus_tag="VVN06_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5562821.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6511..7554
                     /locus_tag="VVN06_001277"
     CDS             6511..7554
                     /locus_tag="VVN06_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU5562822.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYKVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7618..9303
                     /gene="lpdA"
                     /locus_tag="VVN06_001278"
     CDS             7618..9303
                     /gene="lpdA"
                     /locus_tag="VVN06_001278"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5562823.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9367..10356
                     /locus_tag="VVN06_001279"
     CDS             9367..10356
                     /locus_tag="VVN06_001279"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU5562824.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAKINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10429..11499)
                     /gene="xerS"
                     /locus_tag="VVN06_001280"
     CDS             complement(10429..11499)
                     /gene="xerS"
                     /locus_tag="VVN06_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU5562825.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3392 a         2174 c         2612 g         3349 t
ORIGIN      
        1 ttcaaatcgt atatagtcga tttattaaat ctttagataa attgtaaaaa gtggtttcca
       61 tagccacttt ttgctataat agaggcagta aaaatgaagg agtcagctat ggaacactcg
      121 tcttggcatg ctttgattaa ggcgcaatta cctgagggtt atttcgggaa aatcaatcag
      181 tttatggagc aggtctattc tcaggggatt atttatccac ccaaggaaaa ggtttttcag
      241 gctctcttga caacactgct tgaagaagtt aaggtggtaa ttctagggca agacccctat
      301 cacggaccag gtcaagcgca gggcttgagt ttttctgtac ctgactctat cccagctcca
      361 ccatccttgc aaaatatctt gaaagaattg tcagatgata tcggagttaa gaaatctcat
      421 gatttgacag cttgggctga gcaaggagtc ttgcttctta atgcttgttt gactgttcct
      481 gctggacagg ccaatggtca tgctggtcaa atctgggagc cttttactga tgctgtgatt
      541 caggtggtca atcatctaga tagaccagtc gtttttgtac tctggggagc ttatgcacgt
      601 aagaagaagg ccttagttac caatcctcat cacttgatta tcgaatcagc ccatccaagt
      661 cctttgtcgg tttatagagg attttggggt tccaagccct tttccaaggc caatgcattc
      721 ttaaaagaga caggacaaga gccaatcgat tggcttagat aaggagaaaa tatgcctcag
      781 ttagcgacga tttgctacat tgataatggg aaagaactgc tcatgctcca tcgtaataag
      841 aaacccaatg atgtccatga agggaaatgg attggtgtgg gtggtaagct agagagagga
      901 gagacgcccc aggaatgcgc ggcgcgtgaa atccttgaag aaacagggct caaagccaag
      961 ccagttctaa aaggtgtcat cacttttcct gaatttacag cagatttaga ctggtacacc
     1021 tatgttttta aggtgacgga gtttgagggc gacttgattg actgcaatga ggggacgcta
     1081 gaatgggttc cctatgacga ggttttgagc aagccgactt gggaaggtga ccacaccttt
     1141 gttgagtggc ttttagagga taaacccttc ttttcagcca agtttgttta tgatggggat
     1201 aaattgttgg atacccaagt tgatttctat gaataaagga gaaagcagat gctactaatc
     1261 aaaaatggtc atgtaatgga tcccaagtct ggtttggatc aagtttgtga tgtcttagtt
     1321 caagatggga aaattatcaa aattgcgcct gagatcacgg aagaaggagc agaaacgatt
     1381 gatgctactg gtcttgtggt tgctcctggt ttggtcgata ttcatgtgca tttccgtgaa
     1441 cctggtcaaa cacataaaga agacattcat actggtgccc tagcagccgc tgcaggtggt
     1501 tttactactg tcgtcatgat ggctaatacc agtccaacca tttcagacgt ggagactttg
     1561 caagcagttc tccagtcagc tgccaaagag aagattaatg tcaagacagt tgcgaccatt
     1621 actaaaaact ttaatggtaa aaacttgact gactttaagg cgctcttaga agctggtgcg
     1681 gttggtttct ctgatgacgg tattccgctt gagagcagta agattgtcaa ggaagccatg
     1741 gaggaagcca aaaaactcaa tacctttatc agccttcatg aggaagatcc aggtttgaac
     1801 ggtgttcttg gctttaatga aaatattgct agagaacatt tccatatctg cggtgctact
     1861 ggggtggctg agtacgctat gatggcgcgt gatgtcatga ttgcctatgc aactaaagcc
     1921 catgttcaca tccagcattt gtctaaggaa gaaagtgtta aagtagtgga gtttgctcag
     1981 gggttaggtg cagaagtcac agcagaagta gcgccacagc atttctctaa gacagaagca
     2041 cttcttttaa cacaaggtag caatgctaag atgaatccac cgcttcgttt ggaatcagac
     2101 cgtcgtgccg ttatcgaagg tctcaaatca ggtgtcatca cagttattgc gactgaccac
     2161 gcgcctcatc atgtagatga aaaaaatgtt gaggatatta ccaaagcgcc atctggtatg
     2221 actggcttag aaacatctct gtctctcggc ttgacctatt tagtagaagc tggtgagttg
     2281 agcttgatgg aattacttga aaaaatgaca tacaacccag ccaagcttta caactttgaa
     2341 gcaggttact tggctgagaa tggtccagca gatatcacta tttttgatgc caaggctgac
     2401 cgccttgtgg actcccattt tgcttccaaa gcagctaatt caccattcat cggtgaaacc
     2461 ttaaaagggc aggttaaata taccatctgt aagggacaaa tcgtctatca agcttgatag
     2521 gagttagaat atgaattatt ttcgaaaacg tagggagaga caagcgaaat ctaatagtgg
     2581 aatttactgt cccgcagcta atcgttccaa aattcaatat gaaactaaag aaaaagctga
     2641 ccatgcggtt caatatgata acggaggttt agttagaagc tattattgtc gtacatgtgc
     2701 gtgttggcat actacttcaa aatctaataa acctccgcta agtttgaaaa actatggtct
     2761 gaaactcttt ggtttagata aatcagaata gaaagagaag tatgtataag acaaagtgtt
     2821 tacgagagaa gttagtatta tttttaaaaa ttttcttccc aatcctgatt taccaatttg
     2881 ccaattattc tgcctctttt gttgatactg caatgacagg tcaatacaac actatggact
     2941 tggctggtgt atctatggca accagtatct ggaatccttt ctttacattt ctaacaggga
     3001 ttgtgtcagc cttggtgcct atcattggtc accatcttgg tcgaggcaaa aaggaagaag
     3061 ttgcgtctga tttttaccaa tttatttatt tggccttggg cctatctgtg gtcttgctgg
     3121 ggatggtact tttcttggca ccaacaatct tgaatcatat tgggttagaa gcagtagtag
     3181 cggcagtagc ggttcgctat ctttggtttt tatctatcgg gattatcccc ttgttgctct
     3241 ttagcgtcat tcgttccttg ctggattcgc tgggcttgac caaactgtcc atgtacctca
     3301 tgcttttgtt actccctctc aatagcggat ttaactatct cttgatttac ggtgcctttg
     3361 gtgttccaga actgggaggg gctggtgctg gtttaggaac atccttggcc tactgggtct
     3421 tgcttgggat ttctgttctg gttttattta aacaggagaa gctcaaagcc ttacaccttg
     3481 agaaacgaat tccacttaat atggataaca ttaaggaagg agttcgttta ggtctgccta
     3541 ttgggggaac tgtcttcgcg gaagtggcta tcttttcagt ggttggcttg attatggcta
     3601 agttttcgtc cttgattata gctagtcacc agtcagctat gaacttttca agtcttatgt
     3661 acgcctttcc tatgagtatc tcatcggcta tggctattgt cgtttcctat gaagtgggag
     3721 ccaagcgatt tgatgatgcg aaaacctata ttggtctagg aagatggact gctctcattt
     3781 ttgcggcctt caccctaacc ttcctttaca tttttagggg aaatgtggcc agtctttatg
     3841 gtaacgaccc aaaatttatc gatttgacag cgcgtttttt aacttatagt cttttcttcc
     3901 agttagcaga tacctttgcg gcaccgcttc agggaatttt gcgggggtat aaggatacag
     3961 ttattccttt ttaccttggt ttgcttggtt attggggcgt agcaatccct gtggctacgc
     4021 tatttgattc cctaacagat tttggagctt attcttactg gatcggcttg attattagtt
     4081 tgattgtgag tggggcgctc taccgttggc gtttaactgt gattatgaag agatttgaat
     4141 ctttagcaaa atccaaacgc taaaaagttt gtgaaaaaat attcttgaca agaaaatccc
     4201 ttgttttcac ggggttttct tttttatttt gtgaaatttc ttttagaaaa actttgacag
     4261 tatttgccaa aagcggtaaa atagtaaggt aagaagaaag ttagaaaggc aagaagatgt
     4321 caactttaga taaaaatctt ttgctagaaa tgttccgtaa gatggaagaa atccgtcgca
     4381 tggacttaaa aattgcacaa ttagtaaaga aagggaaagt gccaggaatg acgcactttt
     4441 ctgttggtga agaggcagct aacgtggggg ctatgttagc tctcaatcca gatgatctga
     4501 ttacctcaaa ccaccgtgga cacgggcaag ctattgctaa agggattgac ctcaacggaa
     4561 tgatggctga aatccttggt aaatacactg gaacctgtaa agggaaaggt ggatctatgc
     4621 atatcgctga ccttgatgct gggaaccttg gtgccaatgg tatcgtaggt ggtggtatgg
     4681 ggatcgctgt cggtgcagcc ctcagtcagc aaatgcaaaa taccggtaaa atcgttgtct
     4741 gcttctttgg agatggtgcg accaatgaag gtgttttcca cgaagcagtg aacatggctt
     4801 ctatctggaa cctgccagtc attttctatt gcattaacaa cggttacggt atctctgcgg
     4861 atatcaagaa aatgaccaat atagaacata tccatcaacg tagcgccgct tatggaattc
     4921 ctggaatgtt catcgaagac ggtaacaatg tcatcgatgt ctatgaagga tttcagaaag
     4981 ctgtagacca tgttcgcagt ggcaatggtc cagtcttgat tgaaagtgta acttatcgct
     5041 ggctcggtca ctcatcatct gaccctggta aatatcgtac gcgtgaagaa gtggaattgt
     5101 ggaaacaaaa agatccaatc gaaaacctcc gcaattacct tattgaaaat aacattgcaa
     5161 gtgccgaaga attggaagaa atccaagcgc aagtaaagga agcagtagaa gcttctgtta
     5221 aatttgcaga ggaaagccca ttcccaccgc ttgaatcagc ctttgaagat atttacgcag
     5281 actaaggaga aagagataaa atggaaacaa aaacaatgtc cttccgtgac accattatcc
     5341 ttgctatgtc tgaggaaatg cgtcgcgatg aaaatgtgtt cttgatggga gaagacgtcg
     5401 gtgtcttcgg aggagacttc ggtacttctg ttggaatgct tgaagaattt ggtccagaac
     5461 gtgtccgtga ctgtccgatt tctgaagctg ccatctctgg agcagcagca ggagcagcca
     5521 tgacaggact tcgtccaatc gtcgatatga ccttcatgga cttctctgtt attgccatgg
     5581 acaatatcgt caaccaagct gctaaaacac gttacatgtt tggtggtaaa ggtcaggttc
     5641 caatgactgt tcgttgtgca gctggtaacg gagttggctc tgccgcccag cactcacaat
     5701 ctctagagtc ttggtttact cacattccag gacttaaggt tgtggcacct ggaacacctg
     5761 cggacatgaa aggcctgctt aagtcttcta tccgtgataa caacccagtt attatacttg
     5821 agtacaagtc agaatttaac caaaaagggg aagtgccagt tgatccagac tacacaattc
     5881 cacttggggt tggtgaaatc aaacgccaag gaacggatgt aacagttgtt acttatggaa
     5941 aaatgcttcg ccgtgtggtt caagctgctg aagaattagc agaagaagga atttcagttg
     6001 aaattgttga cccacgtacc cttgttccgc ttgataagga tatcatcata aactcagtga
     6061 agaagactgg taaggttgtt ctggtcaacg atgcccacaa aacaagtggc tatatcggtg
     6121 aaatttcagc tattatttca gaatcagaag catttgacta tctagatgca ccaattcgcc
     6181 gttgtgcagg agaagatgtg ccgatgcctt atgcacaaaa cctagaaaat gcaatgattc
     6241 caacagttga aagtatcaaa gatgcaatcc gaaaaactta taacaaagaa tagagttaca
     6301 taagataagt tatgtaaata aaataaagct gacgagaaac tttgtatctt ttaggtgcag
     6361 agctcttcgt cattaatatc ttagattaga gcacgggcta aaagctcgga aaaaagataa
     6421 atctccttgg cttcatcgaa gccatcgtcg atttcctatt tttcagtcgc ttttgacgcc
     6481 ctttgtatca taaatagaat tggagaggtc atggctgatg acaagctaag agcgactcct
     6541 gcagctagaa agttagcgga tgatttaggg ataaacctct acgacgtttc tggctcaggt
     6601 gcaaacggtc gtgtccacaa agaagacgtg gaaacttata aagacacaaa cgtggttcgc
     6661 atttcgccac ttgcaaaacg aattgccctc gaacataaca ttgcttggca ggaaatccaa
     6721 ggaaccggtc atcgtggtaa aatcatgaag aaggatgttt tggccctgct tcctgaaaat
     6781 atcgaaaacg atagcatcaa atctcctgct cagattgaaa aagtggaaga agtccctgat
     6841 aacgtaacac catatggtga aatcgagcgt attccaatga caccaatgcg taaggttatt
     6901 gcccaacgca tggttgaatc ttacttgact gcgccaacct tcaccctcaa ctataaagtt
     6961 gatatgactg aaatgttggc tcttcgtaag aaggttcttg agccaatcat ggaagcaact
     7021 gggaagaaga ctactgtaac agaccttctt tcgcttgcag ttgttaagac tcttatgaaa
     7081 cacccataca tcaacgcttc attgacagaa gatggcaaga ctattatcac tcacaactat
     7141 gtcaatcttg ccatggcagt tgggatggat aatggattga tgacacctgt cgtttacaat
     7201 gctgagaaaa tgagtctttc agaactggtt gtagccttta aggatgttat tggccgtacc
     7261 ttggatggta aattggctcc aagtgagtta caaaattcaa cattcacaat cagtaatttg
     7321 ggaatgtttg gtgttcagtc ctttggtccg attattaacc aacccaactc agctatcctt
     7381 ggtgtcagtt cgacaatcga gaagccagtt gtcgtcaatg gtgaaattgt gattcgccca
     7441 atcatgagtt taggattaac cattgaccac cgtgtcgtag atggtatggc tggtgctaag
     7501 tttatgaagg acttgaaaga gttgattgaa acgccaatct caatgttaat ttaagagcaa
     7561 gagtattcat ttttaagata tagataaagg aaggaagaca tggccttaga agtaattatg
     7621 ccaaaagccg gcgtggatat gacagaagga caaatcgtcc aatggaataa aaaagtcgga
     7681 gaatttgtaa aagaaggaga aatccttttg gaaatcatga ctgataaagt cagcatggaa
     7741 ttggaagccg aagaagatgg gtacttgatt gccattctca aaggagatgg tgaaactgtc
     7801 cctgtaacgg aagttatcgg ttaccttggt gaagaaaggg aaaacatccc aacagctgga
     7861 gcagcatcgc cagaagctag ccctgtacct gtagcaagta cctcaaacga tgatggtaag
     7921 agcgatgatg cttttgatat cgttgtgatt ggtggaggtc ctgctggtta tgttgcagcc
     7981 attaaagctg cccaattcgg cggtaaggtt gcccttgttg agaaatctga acttggtgga
     8041 acctgcttaa accgtggatg tattccaacc aagacctacc ttcataacgc tgaaattatt
     8101 gaaaatatcg gtcatgctgc aaatcgtggt atcgtgattg aaaatcctaa cttcactgtt
     8161 gatatggaaa aacttttaga aactaaatct aaagttgtta atactcttgt tggtggtgtt
     8221 gcaggacttc ttcgtagcta cggagttact gttcataaag gaattggtac aatcactaaa
     8281 gacaagaacg tcttggtaaa tggttctgaa ttgcttgaaa ccaagaaaat cattcttgct
     8341 ggtggttcaa aagtcagcaa gatcaacgtc cctggtatgg aatctccact tgtcatgact
     8401 agtgatgaca ttcttgaaat gaacgaagtg ccagaaagcc ttgttatcat cggtggtgga
     8461 gttgtcggta tcgaactcgg tcaggccttc atgacatttg gttcaaaagt gactgttatc
     8521 gaaatgatgg accgtatagt tccagctatg gatgtggaag tttctaagaa ccttcgcttg
     8581 attcttgagc gtaaaggaat gaccatcttg actggtacta aactgcaaga aatcattgag
     8641 gaaaatggtc aacttcgtat caaggttgaa ggaaaagacg atatcatcgc aagcaaagct
     8701 cttctttcaa ttggtcgtat gccagacctt gaaggtattg gagaggttga gtttgaattg
     8761 gatcgtggtt gtatcaaggt caacgaatac atggaaactt cagttccagg catttatgca
     8821 ccaggtgaca tcaacggtac taagatgttg gctcacgcag ctttccgcat gggtgaagtt
     8881 tccgctgaaa atgcacttaa aggaaatcat gcagttgcca aattgaattt gactcctgca
     8941 gccatctaca ctctccctga agtagcagca gtaggtttga cagaagaaca agcccgtgag
     9001 aaatacgatg ttgccatcgg taagtttaac tttgctgcta acggtcgtgc tattgcatct
     9061 gacgcagctc aaggtttcgt aaaagttatc gctgataaga aatacggaga aatccttggt
     9121 gtacacatta ttggtcctgc agccgcagaa ttaataaacg aagcatcaag catcatcgaa
     9181 atggaaatca ctgttgagga aatgctgaag accatccacg ggcacccaac ttactctgaa
     9241 gtgatgtacg aagcgtttgc agatgttcta ggaatggcca tccattcacc taagaaaaaa
     9301 taaaacgaag atagattaga ctggaaggtt atttccagtt tctatcatat aaagtaaagg
     9361 gatatcatga aatacattat caatcattca aatgacactg cttttaatat tgccttggaa
     9421 gaatacgcct ttaaacacct tttggatgag gatcaaatct tcctactttg gattaacaag
     9481 ccatctatca ttgttggtcg tcaccagaac actatcgaag aaatcaaccg tgattatgtt
     9541 cgcgaaaatg gtattgaggt agttcgccgt attagtggtg gtggagctgt ttaccacgat
     9601 ttaaataacc tcaactacac gatcatctcc aaagaagatg aaaataaggc atttgacttc
     9661 aagagcttct caactccggt tatcaatacc ctggctcaac ttggggttaa agctgagttc
     9721 acaggtcgta atgaccttga gattgatggt aagaagttct gtggcaatgc ccaagcctat
     9781 atcaacggcc gtatcatgca ccacggttgc ttgctctttg acgttgattt gtcagtcctc
     9841 gcaaatgccc ttaaggtttc aaaagataaa tttgaatcaa aaggtgtgaa atccgtccgt
     9901 gcccgtgtaa ccaatattat caatgaatta ccaaaaaaaa tcacagtcga aaaattccgt
     9961 gatttactct tggaatacat gaaaaaagag tacccagaga tgactgaata cgtcttttca
    10021 gaagaagaat tggccaaaat caatcacatc aaggatacta agtttggaac ttgggactgg
    10081 aactatggta aatcacctga atttaacgtc cgtcgtggaa ccaaattcac tagtggtaag
    10141 gttgaagtct ttgctaacgt tactgaatca aaaatccaag acatcaagat ttatggtgac
    10201 ttctttggta ttgaagacgt tgctgcagta gaagatgtcc ttcgtggggt gaaatacgaa
    10261 cgcgaagatg ttcttaaggc gctaaaaacc attgatatca cacgctactt cgctggtatt
    10321 agccgtgaag aaatcgctga agcggtagtt ggataaatca aaaagaagtt ggccaactaa
    10381 ccaacttctt tttttgatat ttatttacat aatttaaaat acgtaaaatt ataaactatc
    10441 tagagcattc ttttgttcat cattaacaat atgggtatag aggtcagtga cttgtgtgct
    10501 ggcatgtcct agttggtgac tgactaaaac ttgtgattta gtcgcatcat agagcctagt
    10561 tgctagcgta tggcgcagtt tatggggtgt tacacgcact ttaaaatcct ctgagtattt
    10621 agcaaccatt ttctcaacgc tagaagcatc gatacgatta ggaacacctc tgtagagagt
    10681 taaaaaaagg gctgtatctg ttttttccgt tttatagcgt tgattccgaa tggccagata
    10741 attctctaaa taaggtttag caaaagcagc gacattgact gagtcacgtt tgccaccttt
    10801 tcgagtaaca tcaataacca tcatttttag attgagatct cttagatcta gattaacagc
    10861 ttcagataag cgaacaccag atgccaagag aagggcaata atggctaaat ctcgttcttt
    10921 atttttgttg aatgatgaga gagctcgatt tgaaagctgt tgtgggtgct cttgatcgat
    10981 ataagttaga aaaccttctg tttcatcacc tagaaagagt ttttgcttga tattttcagc
    11041 tctggcagca agggtttctt tctttttctt ggttgaaact tttttcatta cattacgata
    11101 gaaataaggt tccccctgat cgttttcaac ctcctcggtt agatacttgt aaagactaga
    11161 aagtgctgat aaggttcgat tgatagttgt ctgtgaaaca ccctgttttg ttgtattagc
    11221 attcagcaag ggacgttcac gtagataaag gataaaggat tccatgtctt tcttagacat
    11281 attttccaaa actgataaag gaatatcgga tattttatca gcgtttgaaa taccagactc
    11341 caaaacccag ctgaaaaatc ggtcatattc cttaaggtat tcgtacaggg ttgtaaaact
    11401 gtagggtaca gccagcttag attggtagta ttccagaaca taccagggca tgagttgttt
    11461 tagtttgtcg attcgttcca gtaaaatctc acgtttcatg tattttctcc ataagtaagt
    11521 atactag
//
LOCUS       DAVHIU010000057        11364 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVHIU010000057 DAVHIU010000000
VERSION     DAVHIU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.059"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(134..1588)
                     /locus_tag="VVN06_001281"
     CDS             complement(134..1588)
                     /locus_tag="VVN06_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU5562826.1"
                     /translation="MWELYSRIWELGDQWRKENSYIVNEGKKNERVEIPRPSVAIVAK
                     ALQEICHFTFIGEGVISDISKLYLYHLDLGHYVSSNDIFRKLLLKYDSRLTSNKFFLE
                     LISYIRTETKMKPPLDDYRYIPVANGVYNIKTHKLEEFSPNFVITSKIQTEYNPCARK
                     PILDGWFDFDRWLEALAVNDKEVVALLWQVINEAINPNRTRKKMVLMVGDGNNGKGTF
                     QALLENLIGRSNISNLKPDQFGKEFYLGALEGKVCNIGDDISNKYLDEVSDLMSVISG
                     DPVQVNKKSLQPVEARFRLLCIFSGNDLPRARNKTMGWYRRLCIIPFRADFNGQKERH
                     EIKDRFIKNKELLEWVLFKVLNMPDFDSFIEPEAVQKMLSKYKNDNDYIKVWVENFYI
                     PNGWHEINHVPMFIARNKLKEFAEDIGIDKPKLGQFSNAVMAELEKTTSYKYSAKNGT
                     VSSKFYDVLDPQGFQRKRFVKGIWGIHLEDEDLG"
     gene            complement(1668..2528)
                     /locus_tag="VVN06_001282"
     CDS             complement(1668..2528)
                     /locus_tag="VVN06_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU5562827.1"
                     /translation="MTIYEARGFQSNLVYPFDKIEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNTSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            complement(2525..2797)
                     /locus_tag="VVN06_001283"
     CDS             complement(2525..2797)
                     /locus_tag="VVN06_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562828.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            complement(2790..3131)
                     /locus_tag="VVN06_001284"
     CDS             complement(2790..3131)
                     /locus_tag="VVN06_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5562829.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            complement(3112..3405)
                     /locus_tag="VVN06_001285"
     CDS             complement(3112..3405)
                     /locus_tag="VVN06_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562830.1"
                     /translation="MSYIVKIYLDSEKIPDEPYFTKEIFLATCMGNATKLAVTMGLLT
                     KQALDLETKTAILKALDTIIETQKAILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            complement(3402..3617)
                     /locus_tag="VVN06_001286"
     CDS             complement(3402..3617)
                     /locus_tag="VVN06_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562831.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            complement(3610..3876)
                     /locus_tag="VVN06_001287"
     CDS             complement(3610..3876)
                     /locus_tag="VVN06_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562832.1"
                     /translation="MTKEEIRNILDELDDLTLGLNTAENAFDATFHFFSKNISTKEKT
                     MLLTLEYDTQSAIFNLAYDCLINAKENLVNTIRMYRKELEEKHE"
     gene            complement(3894..4034)
                     /locus_tag="VVN06_001288"
     CDS             complement(3894..4034)
                     /locus_tag="VVN06_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562833.1"
                     /translation="MILDLGKMTQAEFDEVMADIKARNPNLLQLITDFLDRKVTPEEV
                     DD"
     gene            complement(4046..4546)
                     /locus_tag="VVN06_001289"
     CDS             complement(4046..4546)
                     /locus_tag="VVN06_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562834.1"
                     /translation="MISDKLEAISTNLEEIQKSLVGAETALAKREISRVLNDLDDVYS
                     ELTSTEYHEQQQTIKEQTERMKQRVIAEVIGELEEREESYYRNFWGDTQFIKLLSNFD
                     GFLFLNTYLAEITKENTYPMEQSQLLNYVWELIAVDIAKKKRGKKNVLDSWTDSSLYT
                     RGMMID"
     gene            complement(4543..4683)
                     /locus_tag="VVN06_001290"
     CDS             complement(4543..4683)
                     /locus_tag="VVN06_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562835.1"
                     /translation="MEETTYDIIAKSLDRISMELHQADENNDFSRIWVLSGQLKGQWR
                     KT"
     gene            complement(4948..5097)
                     /locus_tag="VVN06_001291"
     CDS             complement(4948..5097)
                     /locus_tag="VVN06_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562836.1"
                     /translation="MTNYDRAKRFYSRATYVLSDFLHGRIDEQEFRKQWQRLADKAVN
                     ETKKN"
     gene            complement(5084..5230)
                     /locus_tag="VVN06_001292"
     CDS             complement(5084..5230)
                     /locus_tag="VVN06_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562837.1"
                     /translation="MEQYTKRASALIITFNRGVISQDEFIEEFTKLREKVVKAVSEAK
                     NDKL"
     gene            complement(5234..5662)
                     /locus_tag="VVN06_001293"
     CDS             complement(5234..5662)
                     /locus_tag="VVN06_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562838.1"
                     /translation="MRKKHQVVKFKDIAEKLPELEGKRLEEITKVLGYRNLESCRTNL
                     CKLKQNNRLDFTLEKGVYSKFALLDGTVKEELEDKELSERGRYLKSVDRYKAMLNAFS
                     IAFDSTVKAETRQKAEHDGLKALDRIPDKHYALLYDMMEG"
     gene            complement(5816..5989)
                     /locus_tag="VVN06_001294"
     CDS             complement(5816..5989)
                     /locus_tag="VVN06_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU5562839.1"
                     /translation="MTDKIKKVGRPKGENSNTVKLTVRVSEQLNSKLEEYGKKNGLTK
                     PETLRKAFENLTK"
     gene            complement(6228..6476)
                     /locus_tag="VVN06_001295"
     CDS             complement(6228..6476)
                     /locus_tag="VVN06_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562840.1"
                     /translation="MENEFKTVTNAKGVEIPKYFKDFKKLVEMDRQLAEYLCMNYELL
                     DSEDLGAFLETVEQGFSWILELIESKDLLYSPQTGKKA"
     gene            complement(6488..7093)
                     /locus_tag="VVN06_001296"
     CDS             complement(6488..7093)
                     /locus_tag="VVN06_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU5562841.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAGVTHHTIQELLRKHKADFESY
                     GIIAFEMRKLDGRGRPMKIYRLNEQQATLLITYLRNTEPVRKFKMNLVKAFFEMRDEL
                     SKRYLQRELEKPKRKTLTEAIKSWEKAPQHAYSTLTNLLLKGATGKNKAQLMQERESE
                     NGIDSLTSAELTNYQRLEDMAIAMINLNMRYSEIKELIFKV"
     gene            complement(7115..7492)
                     /locus_tag="VVN06_001297"
     CDS             complement(7115..7492)
                     /locus_tag="VVN06_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562842.1"
                     /translation="MLYHWGLTFSEEISDLEHKGENNMNNKQVYQAQTTDHGIARALG
                     EEIRMITDVEETTYNGHGEIIVEYTSDVALTKLFDRIKALYEKGESYETKPFLHFKVL
                     EDDIEPSPNGVFTAKEVAEFLGI"
     gene            complement(7567..7758)
                     /locus_tag="VVN06_001298"
     CDS             complement(7567..7758)
                     /locus_tag="VVN06_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5562843.1"
                     /translation="MRLTLKALRANSNMKQSEVAQKLGISTTTWSKWENGKSFPDVAQ
                     VKEIEKLFGVAYDDIIFLS"
     gene            7962..8678
                     /locus_tag="VVN06_001299"
     CDS             7962..8678
                     /locus_tag="VVN06_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562844.1"
                     /translation="MADNIKRLLSAKGLNPRQLAIALDFKYTTVNDWVNAKTYPRIDK
                     IEMLANFFNVSKSDLVENKNAETPTTSLIQSIYDQLTPPRQAKALTYLKKQLLEQKNE
                     NIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNIEKCFYTDEIPDDYDAIAYV
                     VGNSMEPKIKNGDYLFIKNTPQVDYNTIGIFQVDGANYVKKLRQGYLESLNPDCADIQ
                     LDESNDIRTIGEVVGIYREK"
     gene            8697..9365
                     /locus_tag="VVN06_001300"
     CDS             8697..9365
                     /locus_tag="VVN06_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5562845.1"
                     /translation="MKFCPECGNSVVGCKFCPNCGYSINASLKTTPQKVATPSSRPST
                     VRSKRTDKVGPLEIDKNHRTYRIHGARKAKGSSGLIGGTAKVVGKTTLALGTGGLSLI
                     PSLIKKDKNDTDWYSFDDLVSYELVINDQSVVSGGVGQALVAGAVFGVIGAVAGGIVA
                     KRKATTKILNMTIRITSNDFTKPVVFIDLIKKPVKNTSKEYKEAIENAQKIIGALDVI
                     THNS"
     gene            9504..10172
                     /locus_tag="VVN06_001301"
     CDS             9504..10172
                     /locus_tag="VVN06_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU5562846.1"
                     /translation="MTYSQNYLDDILVRMAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLDSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGAEFQTASPAETPYLMTQWADNTAYRLEHAQNEKEVLEILADTHIQIERIHPFSD
                     GNGRTGRLILMYLAMKYLNAPIIIPKDSRAHYMELLANQNVTGLADLFKKSLDYERIR
                     KKQF"
     gene            complement(10636..11094)
                     /locus_tag="VVN06_001302"
     CDS             complement(10636..11094)
                     /locus_tag="VVN06_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562847.1"
                     /translation="MVTDIEQARKNYMADYTPVDLTDAHKLLELAEKLQNEDISINTY
                     ELYKHPEAREKLFWQIAEATYMLLEDTGELQGATPTDEHLKTFVDYLEEKFNNIIKKL
                     IAGTDVAKLQGFLEAVEKDNPHLTKQQKGQLLKDTVVTELFKLWIKRRSK"
BASE COUNT         3088 a         2417 c         1953 g         3906 t
ORIGIN      
        1 tactaaccta aatactcgca aagactttta tatcaacgtt tacaaaggtt agtgtttagg
       61 ttatgtttct attttttgca taacctaaat tgctataacc cttggtgtaa ttggtttttt
      121 agtattaaat ttcctaacct aaatcttcat cttctaagtg tattccccat atacctttaa
      181 caaatctttt acgttgaaaa ccttgtgggt caagtacgtc ataaaactta gagctaaccg
      241 ttccgttttt ggctgagtac ttatatgacg ttgttttttc caattcggcc attactgcat
      301 tgctaaattg tccaagttta ggtttatcta tacctatatc ctcagcaaac tcttttaatt
      361 tatttcttgc aataaacatt ggaacatggt ttatttcatg ccacccgttt ggtatgtaga
      421 aattctctac ccaaactttg atataatcgt tatcgttttt gtacttgcta agcatttttt
      481 gtaccgcttc aggttctata aagctatcaa aatctggcat atttaaaact ttaaacaaaa
      541 cccactctag cagctctttg ttctttataa atcggtcttt gatttcatga cgttctttct
      601 gaccgttaaa atctgcacgg aaaggaatga tacaaagccg tctataccac cccatggtct
      661 tatttctagc tctaggcaaa tcattgcccg aaaagataca caatagtctg aaacgtgctt
      721 ccacgggttg taagcttttt ttattgacct gcactggatc tccactaatt acactcataa
      781 ggtcagaaac ttcatccagg tacttgttag aaatatcatc tccaatatta caaacctttc
      841 cttccaaagc gcctaaatag aactctttcc caaactggtc aggtttcaaa ttactaatat
      901 ttgaccgtcc tattaaattc tccagcaagg cttgaaaagt accttttcca ttattaccat
      961 ctccaaccat tagcaccatt ttcttacgtg ttcggttggg attgatagcc tcatttatga
     1021 cttgccacaa taaagctaca acttctttat cgttaactgc taaagcctct aaccatctat
     1081 caaaatcaaa ccaaccatct aaaataggct ttctggcgca tggattgtat tccgtttgta
     1141 tcttactggt aatgacaaaa ttaggactaa attcctctag tttatgagtt ttaatattat
     1201 aaactccatt cgctactgga atatatcgat aatcgtccag cggtggtttc atcttagttt
     1261 ctgtacggat ataggagata agttctaaaa agaacttgtt agaagtcaaa cgtgagtcat
     1321 attttaaaag caattttcgg aaaatgtcgt tacttgatac ataatggcct aaatccaaat
     1381 ggtaaagata taacttacta atatcactta taaccccctc acctataaag gtaaaatggc
     1441 aaatttcctg taacgctttg gctacgattg caacacttgg tcgtgggatt tctacccttt
     1501 cgtttttctt tccttcattg acgatatagg agttttcctt acgccattga tccccaagct
     1561 cccaaatcct actataaagt tcccacatcg ttctaggagg ttgttcttgc tctctgacct
     1621 ccaagatttc actttttagg tttgctaatt cttctagttc tatggttcta acctctcttt
     1681 ctatactcag cccgtgcaat actgctaaaa gtacggtcta gctcctcaat aggcaatggt
     1741 tcaaccgtca cgctattggc tatctttgtc agttcataag ctgtctctag gtcacaatca
     1801 acccacttat taaagagtaa gcctacaaat cgtgtcagtg ctacgttgcg ccctccttcg
     1861 tctccaaatc cgttgaaaag cgtgtcaata atcctcatag tcattgacct ctggccactt
     1921 actctaggtt tataacgctc agtagttcct tgcttcgctc ttggttcaac cttggggact
     1981 ggataatcaa gtccatgctc tacaatcttt tgataagttg ctgggtcgcc tgttgttact
     2041 ggtagccctt ggagttgtga ccaggttaaa ctggccatgt caaagggtag cccaatcttg
     2101 tcagcaatct cttttactac ctgcttatag gttgcctcgt tcatcacgtt gttagatttc
     2161 accacaaggc gaaaacgggg cttttctatg ctatgtttga tagtcgggta taagatataa
     2221 gagtagccaa acaaagcgct agaaacggct tctatgaagt cctcagtcgt cccctgtata
     2281 tcgtcataat caaggaaaat caaatcccga tagattagac tggtgttgtt tcgtttgtaa
     2341 ctcccgtttt tctccggcat aaccttacca cttaaacaat atggcgcctg ggttcttttg
     2401 tattcctccg gatcagcact ttcagggact accaaaggct taaagcgttc aatgtactgg
     2461 aacggctcaa tcttatcaaa tggatagaca agattactct ggaaacctct agcctcgtaa
     2521 attgtcattc atccaccccc agaaacttcc agatgtcagt tactaaatag taaatcattc
     2581 ttgaaccatc gtccggcggt tgatagcgtc ttaaaccctg actttcccac ttattcagcg
     2641 tgttgtcact aattttcaat tcctctttga gttcaactct atttattaac ttggtcaatc
     2701 ttggcggtac tttctcacgc gcttccaggt atctttccac tacctccaga atgccatgcg
     2761 ctaggtcttg ctcgctttct cggcttaggc taaacatatc cgcccacctc cttcaaggtt
     2821 tctttgtagc tttctaggtc gctattcatc aacaccgcta ggcgcttgct ctcctcctgt
     2881 acttggttgt aaaaagcctt agcgccatct agtaactcgc ctttattcgc tggaataaag
     2941 tacccactaa aaacgccaca tctaacccct acaataggga tgttgtgctt aactactaga
     3001 cgcttgataa tgtcacgtac agttctttct gttagcttgg ttgtcaggct aatttctgac
     3061 cctgttatgg agttctcagc ccctaccttg attagtctta aaactcgctt gtcattctct
     3121 gatagactca tccaacccct cctctccttt gcttttattt acaaaggtta tttgtccgct
     3181 cgctacccct tgcaaaatag ctttttgagt ctctatgatt gtatcaaggg ctttcaagat
     3241 agctgtctta gtttctaaat ccaacgcttg ttttgtcaac aagcccatag ttacagctaa
     3301 ctttgttgca ttgcccatac acgtagctag aaaaatctct ttagtaaaat aaggttcatc
     3361 tggtattttt tcactatcaa ggtatatctt cactatgtaa ctcattccat ccctccccat
     3421 acgttgactc ctgcaagctg gatatatcgc ccatattcag ggtttaaatc ctcgctaggt
     3481 gtttttatcg tctgttggtt ttctcgctca atttgggcgc tttttttgcg gtctcggtgg
     3541 tttaaataaa gcagtaagcc aatcaaaatc acgattaaga cagcagcctg tgtgttactt
     3601 atatctagtt cattcatgtt tttcctccaa ttctttgcgg tacattctga tagtattaac
     3661 tagattttcc tttgcattga taagacaatc ataagcaagg ttaaaaatag cgctctgtgt
     3721 gtcgtattcc aaggttaaaa gcatggtctt ttctttggtg cttatgtttt tgctgaaaaa
     3781 gtgaaaagta gcgtcaaaag cgttttcagc ggtgttaagt cctaaggtta aatcatctag
     3841 ttcgtctaaa atatttctaa tttcttcttt tgtcatggtt gtttcctttt caactagtcg
     3901 tccacctctt cgggtgttac ctttctatct aaaaaatcag tgatgagctg taataggttt
     3961 ggatttcttg ccttgatgtc agccattact tcatcaaatt ctgcttgtgt cattttgcct
     4021 aggtctagta tcattgcatt gcctcctaat caatcatcat tccacgagta tataaggatg
     4081 aatctgtcca tgaatccaaa acgttctttt ttcctcgttt tttctttgcg atatcaaccg
     4141 ctatgagttc ccatacataa ttcaataatt gactttgttc cattgggtag gtattttctt
     4201 tagttatttc agctaggtaa gtgtttaaga aaagaaatcc atcaaagttg gaaagtagct
     4261 ttataaactg agtatcaccc caaaaatttc tgtaataaga ctcttctcgt tcttctagtt
     4321 cacctattac ctcagcaatc accctctgtt tcatcctttc tgtttgttcc tttatcgttt
     4381 gctgttgttc atggtattct gtgctagtta gttcactgta aacatcatcc aagtcgttca
     4441 acactctact gatttctctt ttagctaatg cagtttcagc ccctacaagg cttttttgta
     4501 tttcttctaa atttgttgaa atagcttcta atttatctga tatcatgttt tcctccattg
     4561 gccttttaat tgtcctgata aaacccatat tctagaaaaa tcgttatttt cgtcagcttg
     4621 gtgtaattcc atactaattc tatccaagct tttagcgata atatcgtatg ttgtttcttc
     4681 catactttta cctctgtttc tatgtttgtg taatcgcctt gatggctttt taatgctttt
     4741 ttcctgtcac atgcctcatg ctctcggtcg ccaaacttct gagcgtgggg cttttttgag
     4801 ttgtttctta tacagttttt ttcttgtaag tactagcctc acgctcggag tcgccaaatt
     4861 gaaagcgtga gaaagtacca gtttaaagag ttggcgctct atgcttttca aaaccttttc
     4921 taattgcttg cctgcacttc ggttttctta gtttttcttc gtttcgttta cggctttatc
     4981 tgctagccgt tgccattgtt ttctaaattc ttgttcatct atccgcccat gcagaaagtc
     5041 gctcaggacg taagttgcac ggctgtaaaa tcgcttggct ctatcataat ttgtcattct
     5101 ttgcctcact tactgctttt actacctttt ctcttagttt tgtaaattct tcaataaact
     5161 catcttgaga tataacccca cgattaaaag ttataatcaa ggcgcttgct ctttttgtgt
     5221 attgttccat ctgttacccc tccatcatgt cataaagtag ggcgtaatgt ttatctggga
     5281 ttctgtccaa ggctttcaag ccgtcgtgtt ctgctttttg tctggtttcc gccttgaccg
     5341 tactatcaaa ggcgatagaa aaagcattca gcattgcctt gtagcggtct acgctcttca
     5401 aatagcgccc acgttctgac aattctttat cttccaattc ctcttttacc gtaccgtcca
     5461 agagtgcgaa cttgctataa actccttttt ctagtgtaaa atctaggcga ttgttttgtt
     5521 tcagcttgca gaggttggtt ctgcaactct ctaggttacg gtaacctaat actttagtaa
     5581 tttcttctag acgcttaccc tctaattctg gcaatttttc agctatatct ttaaatttta
     5641 ctacttgatg tttctttctc attctttacc tcatttttgg gtacgcaaaa agcgcactct
     5701 atttctatgt ttgacaaata agagtacgca tgataagata tttacgtacc tcattcgtgg
     5761 ggacggatac tttgcaactt ctcggattgg cgtttgacaa gttgcaaggt attttttatt
     5821 tagtaagatt ttcaaaggct tttctcaagg tttcaggttt tgtcaaacca ttctttttac
     5881 cgtactcttc cagcttacta tttagctgtt cgctaactct gactgttaat tttacggtat
     5941 tagagttttc tcccttcgga cgtcctactt tctttatttt gtcagtcatc ggattgcctc
     6001 ctttcttgac gacataaatt attataatta ttgtcgtctt aaatgtcaac ccctaaaccg
     6061 aaaaaaattt aaaatcttcc cagcgtacct tattttattt tcaatgtact agcggaaacc
     6121 gctgtattat gctataatct aggtatagaa aaatatctat acccttgatc cagtcgcttg
     6181 cttcagtcgc caaacgttca gcaagtgact tttttttatt gtctttttca tgctttcttc
     6241 cctgtctggg ggctataaag caaatcttta ctttcgataa gttccagaat ccagctgaat
     6301 ccctgctcca ccgtttcaag aaatgcacct aggtcttcac tgtccaataa ctcgtagttc
     6361 atacaaagat attcggctag ttgtctgtcc atctcaacta gctttttaaa atccttgaaa
     6421 tacttaggaa tttctactcc cttggcattt gtaactgtct taaattcatt ttccattttc
     6481 tatactccta tactttaaaa attagttctt taatttctga atatctcata ttcaagttga
     6541 tcatcgctat tgccatatct tccaagcgct gatagtttgt cagttccgca cttgttaaac
     6601 tgtcaatacc gttttcactt tctcgctctt gcatgagttg ggctttgttt ttcccagtcg
     6661 ctccctttag cagtaggttt gtaagagtgc tataggcatg ctgaggtgct ttctcccatg
     6721 atttgatagc ttcggttaag gtcttgcgct ttggcttttc cagttcccgt tgaagatagc
     6781 gtttagaaag ttcatcacgc atttcaaaaa aggctttaac caggttcatt ttgaatttac
     6841 gaactggttc tgtatttcgt aagtaagtga ttaacaaagt agcttgttgc tcgttcaagc
     6901 gatagatttt cattggtcgc cctcggccgt ctaatttacg catttcaaat gcgataattc
     6961 cgtagctttc aaaatcagct ttatgcttcc ttaaaagttc ttgtattgtg tgatgagtga
     7021 ctccagcaca ttcagcgatg atctcactcg tagtatacgg ctctttcttg ccgtccatgt
     7081 aaaccagttc catgtttttg ctccttattg ttaatcaaat tcctaaaaac tccgccacct
     7141 ctttagcagt aaaaactcca tttgggcttg gttcaatatc atcttccaat actttaaaat
     7201 gtaagaatgg ttttgtctcg tagctttcgc ctttttcgta aagtgcttta atacggtcaa
     7261 atagttttgt caatgcaacg tcactggtgt actcaacaat gatttcaccg tgtccattgt
     7321 aagttgtttc ttcaacgtct gtgatcatac gaatttcttc gcctaatgct ctagcgatac
     7381 cgtggtcagt tgtttgtgct tgatatactt gtttattgtt catgttgttt tctcctttat
     7441 gttctaggtc ggatatttct tccgagaagg tcaatcccca gtggtaaagc accagtggag
     7501 tatcttgtag atgtaatata gtattgcgat tgggttactc ctttctatat ccgtttaaat
     7561 cggatattag cttaaaaaaa taatgtcgtc ataagcaaca ccgaaaagtt tttcaatctc
     7621 ttttacttgg gcaacatcag gaaaactttt tccattttcc cacttactcc aagtagttgt
     7681 agaaattcct aatttttgtg ctacctctga ttgtttcatg ttgctattag cccgcaacgc
     7741 tttcaatgta agacgcaaat agccctcact cctttctttt ggatatccgt ttaaatcgga
     7801 tacaatgaca gtatacggta aaaccgataa cttgtcaaca ctttttttga ttttattttc
     7861 tttttttttg tttcccttgc tttttctacg ttttaaccgt ataattaaat tatgaattat
     7921 agaaaggcgg ttgcactatg tcattaggaa ataagcaaat aatggcggac aatatcaaaa
     7981 gacttttaag tgcaaaaggt ttaaaccctc gtcaattagc tattgcatta gattttaagt
     8041 atacaaccgt caacgattgg gtaaatgcta aaacttaccc acgtatagat aaaatcgaaa
     8101 tgctggctaa ttttttcaac gtttcaaagt ctgacttagt agaaaataaa aacgcagaaa
     8161 cacctaccac ttccctcatc caatccatct atgaccaact cactccacca agacaagcca
     8221 aagccttgac ttatcttaaa aagcagctgc tggagcagaa aaacgaaaac atagtatcag
     8281 aaaacattat cagtctggac gactataggg aaagcaagac gctccctgtt atcggagttg
     8341 taaccgccgg aaacgggata acccaagacg ataacctaaa catagaaaaa tgcttttata
     8401 ctgatgaaat accagacgac tatgacgcta tcgcttatgt cgtcggcaac tccatggagc
     8461 caaagataaa gaatggcgac tacctattta tcaagaatac acctcaagtt gactataaca
     8521 ctatcggtat cttccaagta gacggcgcta actacgtcaa gaaactgcgt caagggtatt
     8581 tagaaagcct taaccctgat tgtgctgata ttcaactaga cgaaagcaac gatatacgca
     8641 ccatcggcga agtcgtgggc atttatagag aaaagtaaaa aaggagtaaa aaaattatga
     8701 aattttgtcc tgaatgcgga aattctgttg tcggttgcaa gttttgtccg aattgcggat
     8761 actcaatcaa tgcctcgctt aaaacaactc cccaaaaagt agcaactcca tcgtctagac
     8821 cttctactgt aagaagtaaa cgtactgata aagtcggacc tcttgaaata gacaaaaatc
     8881 atcgtacata tagaatacac ggagcaagaa aagcaaaagg ttcatcaggt ctgattggcg
     8941 gaactgctaa agttgttgga aaaactacct tagcgcttgg gacaggcggt ttgtctttga
     9001 ttccatcttt aattaaaaaa gataaaaatg ataccgactg gtactccttc gatgatttag
     9061 tatcttacga attagttata aacgatcagt ctgttgtttc cggcggtgtt ggacaagctt
     9121 tagtagctgg ggcggtattt ggtgtaatag gagccgtagc tggcggaatc gtcgctaaaa
     9181 gaaaagcaac taccaagatt ttgaatatga caatccgaat aacatctaac gattttacta
     9241 aaccagttgt ttttatagat ttaattaaaa aacctgttaa aaacacatct aaagaataca
     9301 aagaagctat tgaaaatgct caaaagatta ttggagcttt agatgttatt acccataatt
     9361 cataaacaaa aatataccac ctcatcgaat accccctttt ttcatggtct attgtgcaaa
     9421 aacggggaaa attgaagaat agaaagccga tttacagact aaagcgtaaa attgggcaaa
     9481 agtgacaaat agaaaggaga attatgacct actcacaaaa ctatctggac gatatactag
     9541 tacgcatggc ctaccattct agcggtattg aagggaatac tatctctcta cccgaaaccg
     9601 tcagcattat cttagaaagc accctgccac gcaacggcaa aagtattcgt gagttttatg
     9661 agattgaaaa ccataaacag gccttttctt atctcttgga ctcattggca aaccatcaag
     9721 ctcttactgt ggggcttgtt caagattttc acgccttatt agtggatagg ctgcaacatg
     9781 atcgcggaca atttaagcaa gttcaaaatg ccattatcgg ggcggaattt caaacggcta
     9841 gcccagcaga aacgccctac ttgatgacac aatgggcgga taatactgct tatcgtttgg
     9901 agcatgctca gaatgaaaaa gaagtattag agattttagc agacacccat atccagattg
     9961 aacggattca cccatttagc gatggtaacg gtcgtactgg tcgcctgata cttatgtact
    10021 tggctatgaa gtatctaaac gctcctatca tcatcccaaa agactcaagg gcgcattata
    10081 tggaactcct cgcaaaccaa aatgtaacgg gactagctga cctcttcaaa aagtcactgg
    10141 actacgaaag aatacgaaaa aaacagtttt aaaacaacaa aaaaaaaaga caagctagct
    10201 gctgctgact tgtctaatat cgcctgaact gaaataacgg gaaagattca ggcgattagt
    10261 agcatcttga gagattggaa gcagaaaggt ctggttactg ttgagttggg actctttcaa
    10321 acctcttagt ctacgctatt attataccac tcttgcattt aaaaagtgac tgacggcatt
    10381 tctcacgctc atcagtctat aattctctaa tgaagtataa tgcggtataa tttagacgat
    10441 tttcaacact tcggcatttt agaaacgctt aaagcgctct aaacaatatc aaaaaaaata
    10501 aaactcttga aatattgggg cttgtatgct agaatgaaag cataaaggga aaaacagtgc
    10561 ttctagcata tgtaactaaa aagccccacc gttacagcga tggggttttt tgctgccctc
    10621 ttaaagggtt aaatgctact tgctacgtct ctttatccat agcttgaaaa gctcagttac
    10681 taccgtatct tttagtagtt gccctttctg ttgttttgtt agatgcgggt tgtctttctc
    10741 tacagcctct aaaaagcctt gtagttttgc tacgtccgtg cctgctatca atttctttat
    10801 gatgttgtta aacttctctt ctaggtagtc cacaaatgtt tttagatgtt catctgtcgg
    10861 cgttgcgcct tgaagttctc ctgtatcttc taatagcatg taagtagctt cagcaatctg
    10921 ccaaaataat ttctctcttg cttctggatg tttgtataac tcgtaagtgt tgatacttat
    10981 atcctcgttt tgtagctttt cagctagttc tagtagtttg tgtgcgtctg taaggtctac
    11041 tggcgtatag tctgccatgt agttctttct tgcttgttca atgtctgtta ccatgcttac
    11101 ctctcttgag tgcctctaaa gaaaaagtga tgcccgtttc cgccttttct ttttactggt
    11161 tttattatag cataccccca ctttttaaaa gagtgggggg gtgctcttta atagtcttaa
    11221 tagttttaat agttttaata gtattgaaaa ttttgacccc ttagaaaagc agtaatatca
    11281 agggtttaca agttcgctac ctcgacgatt accccccttt tttgtacctt tttagtataa
    11341 atttgtagta attcttttta aaaa
//
LOCUS       DAVHIU010000058        11269 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVHIU010000058 DAVHIU010000000
VERSION     DAVHIU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.060"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..187
                     /locus_tag="VVN06_001303"
                     /pseudo
     CDS             1..187
                     /locus_tag="VVN06_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            311..1567
                     /locus_tag="VVN06_001304"
                     /pseudo
     CDS             311..1567
                     /locus_tag="VVN06_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1608..1745
                     /locus_tag="VVN06_001305"
     CDS             1608..1745
                     /locus_tag="VVN06_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEU5562848.1"
                     /translation="MPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKI
                     F"
     gene            complement(1883..3187)
                     /gene="eno"
                     /locus_tag="VVN06_001306"
     CDS             complement(1883..3187)
                     /gene="eno"
                     /locus_tag="VVN06_001306"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5562849.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            3351..3797
                     /locus_tag="VVN06_001307"
     CDS             3351..3797
                     /locus_tag="VVN06_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU5562850.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKIWEVKKEEPAKTSLWKKWFS"
     gene            complement(3794..4909)
                     /locus_tag="VVN06_001308"
     CDS             complement(3794..4909)
                     /locus_tag="VVN06_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU5562851.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(5023..5247)
                     /locus_tag="VVN06_001309"
     CDS             complement(5023..5247)
                     /locus_tag="VVN06_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5562852.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            complement(5192..5563)
                     /locus_tag="VVN06_001310"
     CDS             complement(5192..5563)
                     /locus_tag="VVN06_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU5562853.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(5622..7055)
                     /gene="glgA"
                     /locus_tag="VVN06_001311"
     CDS             complement(5622..7055)
                     /gene="glgA"
                     /locus_tag="VVN06_001311"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU5562854.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(7052..8191)
                     /gene="glgD"
                     /locus_tag="VVN06_001312"
     CDS             complement(7052..8191)
                     /gene="glgD"
                     /locus_tag="VVN06_001312"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU5562855.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(8181..9323)
                     /locus_tag="VVN06_001313"
     CDS             complement(8181..9323)
                     /locus_tag="VVN06_001313"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU5562856.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(9313..11241)
                     /gene="glgB"
                     /locus_tag="VVN06_001314"
     CDS             complement(9313..11241)
                     /gene="glgB"
                     /locus_tag="VVN06_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU5562857.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3439 a         2490 c         1982 g         3358 t
ORIGIN      
        1 gatgacgagc gacttttttg aagcttggtt tcagaagttt ctcttaccaa cattaaccac
       61 accatcggtt attattatgg ataatgcaag attccataga atggggaagc tagaactctt
      121 gtgtgaagag tttgggcata aatttttacc tcttcctccc tactcacctg agcacaatcc
      181 tattgagact ctataatatt tgtagtgggt aaccccccta tggatattat ggagcctatt
      241 ttattgtaga aaaaaagtcc catatgacct ataatgaaaa gcgacaaaac aactcattag
      301 aaagaatcat atggaacaat tacattttat cacaaaatta ctagacatta aagacccaaa
      361 catcaagatt ctagatatca tcaatatgga tacacacaag gaaatcatcg ccaaactgga
      421 ctacgacgcc ccatcttgcc ctgagttcgg aaaccaattg aagaaatatg actttcaaaa
      481 accttctaaa attccttatc ttgaaacgac tggtatgcct actagaattc tccttagaaa
      541 gcgtcgattc aagtgctatc actgttcaaa aatgatggtc gctgaaactt ctatcgtcaa
      601 gaagaatcac caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa
      661 gatttctatg actgatattg cccatcagct ttccatctca acttcaactg tcattcgaaa
      721 gctcaatgac ttccacttta aacatgattt ttcttgtctt cctgagatta tgtcttggga
      781 tgagtatgct tttacaaaag gaaagatgag cttcattgcg caagatttta acaatctcaa
      841 tatcatcact gttcttaagg gaagaacaca agctgtcatc cgaaatcact ttcttaaata
      901 tgatagagcc gtccgatgtc gcgtcaaaat cattactatg gatatgttta gtccttacta
      961 tgacttagct agacaacttt tcccatgtgc taaaatcgtg ttggatcgct ttcacattgt
     1021 acaacatctt agccgtgcta tgagtcgtgt gcgtgtccaa atcatgaatc agtttcatcg
     1081 aaaatcccat gaatacaagg ctatcaagcg ctactggaaa ctcattcaac aggatagccg
     1141 taaactgagt gataagtgat tttatcgtcc tacttttcgt atgcacttaa ccaataaaga
     1201 gattttaaac aagcttttga gttattcaca agacttgaaa caccactatc agctctatca
     1261 gctcttgctg tttcacttcc agaataagga accagagaaa tttttcggac ttattgagga
     1321 caatcttaag caggttcatc ctatttttca gactgtcttt aaaaccttcc tcaaagataa
     1381 agaaaagatt gtcaacgccc ttcaactaca ctattctaat gccaaactgg aagcgaccaa
     1441 taatctcatc aaacttatca agcgcaatgc ctttggtttt cgaaactttg aaaacttcaa
     1501 aaaacggatt tttatcgccc tgaatatcaa aaaagaaagg acaaaatttg tcctttctca
     1561 agcttagctt ttcttcaacc cactacagtt gacaaagagc caataaaatt cctttaaaaa
     1621 caatcacgga cagggttggt cattccgact ctgaagtcac tacttccatc tacacccacg
     1681 tcacaaagaa catgaaagat gaagcaatca atgtactgga taaagttatg aaaaagattt
     1741 tttaaaaagt tttgtccctt ttttgtcctc taaatacaaa aatagccctt cggataaaat
     1801 ccgaggggct agaaacgttg ttaaatcaac ggccgaactt ttgaatttca tggttcggga
     1861 taaaatagtt cactgaacta ttttattttt taaggttgta gaatgatttc aatccacgat
     1921 attcagctac ttcaccaagt tggtcttcga tacgaagcaa ttggttgtat ttagcgatgc
     1981 ggtctgtacg tgaaagtgaa ccagtcttga tttgtcctgc gttagttgca actgcaatat
     2041 cagcgattgt tgaatcttca gtttcacctg aacggtgtga tacaacagca gtgtaaccag
     2101 cttctttagc catttcgata gcttcaaaag tttcagtaag agtaccgatt tggttaactt
     2161 tgataaggat tgagttagca gcaccttctt ggataccacg tgcaaggtag tcagtgtttg
     2221 ttacgaagaa gtcgtcacca acaagttgta ctttcttacc aagacgttca gtaagagctt
     2281 tccaaccatc ccagtcattt tcatccatac catcttcaat agtgatgatt gggtatttgt
     2341 taaccaattc ttcaaggtag tcgatttgtt ctgcagatgt acgaacagca gcaccttcac
     2401 cttcaaattt agtgtagtcg taaactttac gttctttatc gtagaattct gatgaagcac
     2461 agtcaaatcc gataaatacg tctttacctg gtacatatcc agcagcttca atcgcagcaa
     2521 ggatagtttc aacaccatct tcagttcctt cgaaacgagg agcgaatcca ccttcgtcac
     2581 ctacggcagt ttccaaacca cgtgatttaa ggattttctt aagagcgtgg aagatttcag
     2641 caccgtaacg aagggcttct ttaaatgttg gcgcaccaac tggcaagatc atgaactctt
     2701 ggaaagcgat tggagcgtca gagtgagaac caccgttgat gatgttcatc attggagttg
     2761 gaagaacttt agtgttgaat ccaccaagat agctgtaaag tgggatttca aggtagtcag
     2821 cagcagcacg agctacagcg atagacacac cgaggattgc attcgcaccc aatttacctt
     2881 tgttaggagt accgtcaagt gcgatcatag cacggtcaat agcttgttga tcacgtacat
     2941 cgtagccaat gatagcttca gcaatgatgt tgtttacgtt gtcaacagct ttttgtgtac
     3001 caagaccacc gtaacgagat ttgtcaccgt cgcgaagttc aactgcttcg tgttcaccag
     3061 tagaagctcc tgatggaacc ataccacgtc cgaaagcacc tgattcagtg taaacttcta
     3121 cttcaagtgt tggattaccg cgtgagtcta ggacttcgcg agcgtaaaca tcagtaataa
     3181 ttgacatttt ttactctcct tatgagttaa attttttaca cctctataat accttaaaac
     3241 ccctcctttt tcaagaaaaa acgttatctt tgtgcaactt ttccttaact ttataaagta
     3301 atcgctttct tttgtctgtt ttattctaac ttttatgata tactgttttc atgacagatt
     3361 tatcaaaaca attacttgaa aaagctcatg gtgggttaaa aataaatccg gatgagcaaa
     3421 gacgctatct tggtactttt gaggaaagag ttcttggata tgtagatatt gacacagcaa
     3481 atagccctca gttagaaaaa ggctttttat ttattttaga aaaccttcag gaaaaagcag
     3541 agccactatt tgtgaagatt tcaccaacta tcgaatttga taagcaagtt ttctacttaa
     3601 aagaagcaaa agaaactgat agtcaagcca ccatagtatc tgaagagcat attacttctc
     3661 ctttcggcct ggttattcat agcaatgcac cagttcaagt agaagaaaaa gaccttcgac
     3721 ttgcttttcc aaaaatttgg gaagttaaaa aggaagaacc agccaaaaca tccttatgga
     3781 agaaatggtt tagctaaatc ttgcacatat ttaataagtg cccaatattg gcagccgtgc
     3841 gctccagata gagactggca tttttcaaac tatcttctaa aggttcactt ttctccaaaa
     3901 tagaaaagac agcttggata ttttcaaatg gtaggggagg taaatcttca gcaagactac
     3961 cgcaaatagc aataacagga actccaacag gggttctttt tgcaacacct ataggcgctt
     4021 tcccagcaaa gctttgacta tcaagtcttc cttctccaac aacaaccaag tcagcatctg
     4081 aaactttctt atcaaagttg attaagtcca agcaggtatc aattccagac acgatacttg
     4141 cctgagcaaa ggcacacaaa ccaccagcaa ggcctccacc tgctcctgct cctttaattt
     4201 ctaatgttgc aggtgagaat ttttcataaa aatcttggat cgcctgatct acgactgcaa
     4261 acatagtcgg atgtagacct ttttgattgc caaaagtgta agtcgcacct tgatgaccac
     4321 ataagggact cacgacatct gctagaatat gaatttgaac accttcagga attttatagc
     4381 aattttctgt tgaaacagaa gctaagttta ataaggattg accggaagca ggcaagacat
     4441 ttccatccct atcataaaat tgataaccta aaccagcagc aatccccagt cctccatcat
     4501 tactggccgt gccaccaaca ccgatataaa tatctttaat ccctttagaa atgagatgga
     4561 gaatcaactc tccaatacca caagtttgga tttgaagtgg atttcgtttc tctagcggaa
     4621 tttttccaag accaaccaag tcagctactt caaatagtgc cagttcccct ttttgaaaat
     4681 agcgcatggc ttctttttgt ccaaaagggt ctgtcacttg gatccatttt tcttttaggt
     4741 caagagaatg tcggatagca tctacagtac cttctccccc atcaccaaca gggcagagga
     4801 gacattctac atctgctatc gattgttgga agcctctttt tattgcttca gctacctgtt
     4861 gagctgtcaa gctttcctta aacgaatccg gtgcaattac aatcttcata ttttccctca
     4921 ttctaaacag tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa catgatgtgg
     4981 tatttctttt ttgagcattt ctttggaaca aaaggcgatt cctcactccg ccgacttcaa
     5041 cattaataga ttattgaccc catcaccgat tgccaccgtt ctttctttag aaagttttag
     5101 tttctttctc catttttcca gagtttcttt tttgacctgg ggacttataa tttgtccaac
     5161 taattttcct gttaaaagac cttctttgac ttcaagctgg ttggcagtga aataggcaat
     5221 accaagggat tttgctaatc tctccaacta ttggtgtaaa tccaccagac accagaccaa
     5281 ctaggatgcc attcttttgg agaatagaga tgaactctgg gacatttagc gatagatgaa
     5341 ttgagttgaa gacgttatca aagaccaaaa taggaagacc ttccaacaag gacactcttt
     5401 ttcttaaact gctttcaaag accaactctc ctcgcattgc tcgacttgta atctgcgaaa
     5461 tttccgcctc atgacctgcc tctctcccta aaagatcaat cacttcttct aggattaagg
     5521 ttccatctac atccaaaaca cacaagcctt ttacttgaga catcagttct cctctctaaa
     5581 cagcctaaaa atcgtatgaa gtcatcatac gattttatct attaattaac taaactatgg
     5641 tacaagtcaa ggtatgactt gcaggctgta tcccatgaga agtcactctc catagcttgt
     5701 ttttgtaggt ttctccaaat gtctggatgg tttctataca agtccaatgc tgtttggaaa
     5761 gtccaattta accaataagg agatagattg tcaaagctaa agccagtacc gcttccttcg
     5821 attggattga aagcgcgaac tgtatctcgc aagcctccaa cttcatggac caatggcaag
     5881 gttccataac gcatagccat catttgagac aagccacacg gttcaaaacg acttggcatg
     5941 aggaagaggt cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa agtgatattt
     6001 gttgatagct tgtctgggta aatctgagca aaccatgaga aagctccttc aaaggctgga
     6061 tcgccagttc ccaaaagaac aatctgaaca tcttcttgca agatatggtg aagactttcg
     6121 accaccacat caaaaccttt ttgacgtgtc aaacgagaaa caattcccac cagtggaacg
     6181 tctgctctaa caggcaagcc aactctttct tgcaattttg ccttattttt ggctttccca
     6241 gacaaatctt cctgattgaa atgatagtct aaaagagcat ccgtctgagg attataaaga
     6301 tcagcatcaa tcccattcac gataccagat actttaccag actccatttt aagaatctga
     6361 tccaaattac atccaaactg actagtcata atttcatgag catagctagg tgaaacagtt
     6421 gaaacacggt tcgcatagag aatacctgcc ttcatccagt tcagacagtt gttccatcga
     6481 agggtgccat cagcgtaacg ttcaaagcca actccaaaca aatcacccaa cattccttct
     6541 gaaaattgtc cttggaattc taaattatga atggttaaaa ctgtttcaat gtcctcatag
     6601 gcttgaatcc aacggtattt ttccttcaac aagaaaggaa tcatagctgt atggtagtca
     6661 tgaacatgga gaagatcagg aataaagtca atcctttcca tagcctcaat ggcagccagt
     6721 tggaaaaagg caaagcgttc tccgtcatca aaatcaccgt aaacatgacc acggaagaaa
     6781 taatattgat tgtcaataaa gtagaaggtt acaccattta atactgtttt cttaattcca
     6841 caatactgtc tgcgccaacc aacgctcacc tcaaaatgaa gcacatcttc aatctgactt
     6901 ccaaatttag cctctaccat atcatagtag ggtaaaatca ctgcaacttc gtgcccagct
     6961 tttaccagtg attttggaag agcgccaatg acgtctccca aaccacctgt ttttgaaaag
     7021 ggtgcaccct ctgctgctac aaataaaatt ttcatgaatg aatatcctct gttactttag
     7081 cacctttctt aaccacaact ggatgttctg cagttcctcg aatcacaaca ccaggctcaa
     7141 cttcaacccc tttgtccaag atagcatatt cgacctgagc cccttctcca ataacaacac
     7201 gagggaagag caggctatct ttaaccaagc tatccttatg gacatgaata ttacgtgata
     7261 gaacagaatt agctacttga ccttcaataa tactaccaga ggcaaactga gaagtgctta
     7321 ccttagatgt attagcatag taagttggct cttcgttttt gacctttgta taaatctttt
     7381 ggtttggtga gaaaagagaa tagaattttt gtgattcaag catatcgata ttcgcttgat
     7441 aataagattt aacagagtga atattggcta gatagcccgt gtactcgtag gcgaaagctc
     7501 cctcttttac agccaaatct cgtaaaacat agcgcaattt ctctggatgt tcttttttag
     7561 cttcttcttc caagtgttca atcaaccaag gtgtatcaac gacaaagata tctgtagaca
     7621 tattgaacgt ttcagctgtt gacttgctat caaagagttt atgagaaaga acatggtctg
     7681 tttcatctac atccaagatt gcatttactt ctgaaatatc tttcttagct agttttttat
     7741 aaactacagt gataggctct tttgttgtac tatgtaggtg gaaaacttgg ttcaaatcaa
     7801 tgttaataag aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa cgtttcaaat
     7861 aagtaagaag ctgttggtag tattcttttc caactgtact actttctaca cgggtattgt
     7921 aaattcctag atagtaatgg ctaagaaggg ttgataagcc ccactcgcgt cctgaacgaa
     7981 tatggtcaaa tactgagctg atattatcct gctggaaaat accaaagaca ctacgaacac
     8041 ctgcattagc aaggcttgaa agtgggaagt caatcaaacg atatttccca ccaaatggca
     8101 aacttgctac tggacggtgg tccgtcaatg tcgacatatt gtgaaaacca actgtatttc
     8161 ctaaaatggc agaatattta tcaatcttca tctgttgcta cccccactac ttcattatat
     8221 cctacaactt gtacttcatc tgttccatca atttcgacac cgtcagaaat aatcgcacct
     8281 tcaccaataa tggcacgttt aatcttagct ccttgaccaa tgatagctcc actcatgata
     8341 actgaatcaa ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag gatagaatgt
     8401 ttaacagttc catcaacgaa acatccgtct acaactaatg agtcttccac atgagcattt
     8461 gccccgagga agtttggtgg tgaaatcaag tttcttgagt aaatcttcca ttgacggtta
     8521 cgactatcca aggcattttc tggagaaata tactccatgt tcgcttccca aagtgactca
     8581 atagtaccaa catctttcca ataaccacta aattcgtaag cataaacact ttcacctgac
     8641 tcaaggtaat ttggaatgac atttttacca aagtctgaca tgccaacctt gctcttttca
     8701 gcagcgacta acatattacg aaggcgttgc caatcaaaaa tgtagattcc catagaagct
     8761 tttgtagatt taggttgagc tggtttttct tcaaattcaa caatacgatt gttagcatct
     8821 gtgttcatga taccaaaacg gcttgcttct ttaagaggga cgtctaaaac tgctactgtc
     8881 aagctggcat tattatcctt atgagactgg agcatatcat catagtccat tttgtagatg
     8941 tgatccccag acaaaatcaa gacatactca ggattgacac tgtcgatata gtcgatattt
     9001 tggtaaatag cgtgactagt cccctcaaac caacgatttc cttcacttgc agaataaggt
     9061 tgaagaatag agacacctga attaataccg tctagtcccc agcttgaacc attcccaata
     9121 tggttgttga gagcaagtgg ttgatactgt gtaacgaccc caacattgtg aatccctgag
     9181 ttggcacagt ttgatagggc aaagtcaatg atacggtagc gcccaccaaa ttgcacagct
     9241 ggttttgcga tgctttgagt gagtttaccg agacgagttc cttgcccacc agcaagaatc
     9301 aaagctaaca tttcattttt cattttctac tcctttttgg tttttatttg tgacggtttt
     9361 agtagatttc aagcgacgtt tgattttcca tacacttgct cccatagccg gtagggtaaa
     9421 ggttaaggtc tgctcataat ctttccatag tccttcttgc gtttgaacag tttgattatg
     9481 ttctttccaa acgcctcccc actcttccaa ctcagtattc catacttctt cgtaaattcc
     9541 tgcaacgggt agtccgattg taaaatcttt ccgctcaaca ggtaccatat taaagataca
     9601 gactaacatt tctccctttt tacccttacg aataaaggaa agaacactct ggtctcgatt
     9661 atccgcatca atgatttcaa taccatcata gctggtatca atttcccaca gacagcgatg
     9721 atctttgtaa aactggttta gctgagaagc gaaatacttc atcttagcat tcattgggtc
     9781 ttctaggtta gaccattcca actgttcttc agatttccat tctaggaatt gaccgtattc
     9841 gctacccatg aagagcaatt tcttaccagg gtgacaaatt tggtacgtat agagattgcg
     9901 caagcctgca aattgattgt aacgatctcc ccacatctta tgcatcatac tcttcttgcc
     9961 atgaaccact tcatcgtgcg agaatggcaa gagataattc tccttgaaaa catacataaa
    10021 gctgaaagtc accaggttaa agtcatattt acgatagatc ggatcttctt cgtagaaacg
    10081 gaggatatca ttcatccagc ccatgttcca tttgtagtca aatcctagac caccaatctc
    10141 tttcattccc gtaatcttgg tcgcagacga actttcttct gcaatcatca tcacatctgg
    10201 atattctaac ttaataacct cattcaagcg ctgaaggaaa taataacctt catagttgag
    10261 atttccgcca tctttattag gtgtccatgg agcatcatca tagtccaaat agagcatgtt
    10321 gctaacagca tccacacgaa taccatccaa atgatagaca tcaatccaat gcttaatgca
    10381 agaaattaag aaggactgga cttcattttt tccaaggtca aaattaaggg caccccaacc
    10441 atggttatga gccttattat ggtcttggta ttcaaaagtc ggtgtcccat cataataggc
    10501 taaggcatca tcgttgatgg taaagtgacc tggtacccag tccacaataa ccccaatatt
    10561 atgggtatga cactcctcga caaaatcttg aaactcctct ggtcggccat aagcatgctc
    10621 taaagcgaag taacccataa gctgataccc ccaactcaag cccaaaggat gggacatcaa
    10681 gggcataaac tcaatatgag tatagttcat ttcaacgaga taaggaatga gttcatcctt
    10741 gagctgggca aaactataag gactgccatc agaatttctt ttccatgatc cagcgtgaac
    10801 ttcataaata ttgacaggac gctcttcaaa gccccaacgt tttcttcgtg ccagccaaag
    10861 tccatccttc catttcttct caggaagctc tgttaagatt gcccctgttc ctggacgagc
    10921 ctcatacctg acggcaaaag ggtcaatctt catcagttga tgaccatttt gacgtgtgac
    10981 atgatatttg taaatatgcc cttcttgagc catattggta aagacttccc agaccccaaa
    11041 gtcatttctt accattggaa tctgattttc aatccagttg gtaaaatcac caaccaagtg
    11101 aacagcctga gcattaggtg cccaaacacg gaaggtatag ccatgctctc catttagttc
    11161 ttccctatgt gctcctagat aatgttggag ataaaaattt tcacccgtca taaaggtttt
    11221 taatgcttct ctattatcca tatactcccc ttctcctgta agcgttttc
//
LOCUS       DAVHIU010000059        11103 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVHIU010000059 DAVHIU010000000
VERSION     DAVHIU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.061"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            11..733
                     /locus_tag="VVN06_001315"
     CDS             11..733
                     /locus_tag="VVN06_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU5562858.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VVN06_001316"
     CDS             730..1578
                     /locus_tag="VVN06_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU5562859.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VVN06_001317"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VVN06_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU5562860.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VVN06_001318"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VVN06_001318"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU5562861.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VVN06_001319"
     CDS             complement(3249..5957)
                     /locus_tag="VVN06_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5562862.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMETTANAYLTTAQTLDLAVMSNYGLDQADQEELRQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VVN06_001320"
     CDS             complement(5959..6660)
                     /locus_tag="VVN06_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562863.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VVN06_001321"
     CDS             complement(6910..9321)
                     /locus_tag="VVN06_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU5562864.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGRI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VVN06_001322"
     CDS             complement(9374..10066)
                     /locus_tag="VVN06_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU5562865.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10963)
                     /locus_tag="VVN06_001323"
     CDS             complement(10601..10963)
                     /locus_tag="VVN06_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562866.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
BASE COUNT         3251 a         2539 c         2066 g         3247 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat attctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcggacgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatgt caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ctcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaaccagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaagag ttgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttcct gatgttgctt agatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ttgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg tcttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgtctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat atgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tatttagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagcactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tccttgtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 gtgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataggtatt gccaatcgaa atcggcccat gttctggaga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgacc ggaaagaccg cctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact tttcatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttccg ccccttcctt agtaaaacgc tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcac
     8881 aagtgcctta ctaatattca gctgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct atacggccaa atacttttgc tcctaaagaa ccattaccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgt ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcgtcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga taactgagtc gtcaagtgaa gcgtaacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatgtt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttatttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 acaattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat ttgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gttttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatc gtctgttaca accggtgtat caaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aagagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta acttcaccag aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgctt atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcagctgta tttaccgctg ttcccaacaa gacaaaagga caaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cacttgattt cataataaaa tctccttttt
    10981 tatttttttc aaataaaatt gtatcatatt tgctttttaa aaaaagtatt ttctgatatt
    11041 ataaaataaa aatttgtata atatgaaata aaatataata taatactttt cagaaaccag
    11101 aaa
//
LOCUS       DAVHIU010000060        10881 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVHIU010000060 DAVHIU010000000
VERSION     DAVHIU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.062"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            213..1028
                     /locus_tag="VVN06_001324"
     CDS             213..1028
                     /locus_tag="VVN06_001324"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU5562867.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASDFVNGHILYVDGGILAYIGKQPE"
     gene            1047..1688
                     /locus_tag="VVN06_001325"
     CDS             1047..1688
                     /locus_tag="VVN06_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU5562868.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     NPLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITYNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1719..2720
                     /locus_tag="VVN06_001326"
     CDS             1719..2720
                     /locus_tag="VVN06_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU5562869.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYFLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMGSLFQGISHFHFSGITVAIGQEVRTLLLLLLEEAKRRGI
                     IVSMDLNLRTKMISVLEAKYEFSKFARFADYCFGIDPLMIDDQNLEMFPRDRASLKEV
                     ENRMRQLKEAYGFKAIFHTLRSSDEQDKNVYQAYALGERFEESVQLKTAVYQRVGSGD
                     AFISGVLYQLLHHSYLKTTIDFAVASATLKCTLPGDHLSTSETSIEKLLANAQDIIR"
     gene            2730..3359
                     /locus_tag="VVN06_001327"
     CDS             2730..3359
                     /locus_tag="VVN06_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU5562870.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKQLVDLYQDDQSVCIGAGTVIDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGDFDRISEIAQQYVTLR"
     gene            complement(3739..4112)
                     /gene="tnpA"
                     /locus_tag="VVN06_001328"
                     /pseudo
     CDS             complement(3739..4112)
                     /gene="tnpA"
                     /locus_tag="VVN06_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4460..7663)
                     /locus_tag="VVN06_001329"
     CDS             complement(4460..7663)
                     /locus_tag="VVN06_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU5562871.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRVAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRIYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYKDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESGNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIDNNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYIIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQATQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7886..8362
                     /locus_tag="VVN06_001330"
     CDS             7886..8362
                     /locus_tag="VVN06_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU5562872.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTTTGCGLMPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLISRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(8584..10797)
                     /locus_tag="VVN06_001331"
     CDS             complement(8584..10797)
                     /locus_tag="VVN06_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEU5562873.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSIPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHYFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
BASE COUNT         3207 a         2338 c         1908 g         3428 t
ORIGIN      
        1 tatttttatc ttgtcaatag ttttttcaaa aaaatattag cgctttcatt gcttatttaa
       61 ccattgcaat agacagaaac ttcaaaatct tccgtttttt taggaaaaca aagattttca
      121 gaaataaaaa tatttaatta ttgacaaaac cggtttacta gaatataatt tggttataga
      181 gaagtcttaa caaatttgaa aggaagtatc aaatgacaaa tacatcattc tcaattgagc
      241 agttttcttt aaaaggaaaa attgctctca tcaccggcgc ttcttatgga attggatttg
      301 ctattgccaa atcctacgct gaggccggcg ctactattgt ctttaacgat atcaatcaag
      361 acctggtcaa taaagggatt gaagcttatc gtgaagttgg catcgaagcc catgggtatg
      421 tctgtgacgt gacagacgag gacggtatcc aagccatggt caagcaaatc gaacaagagg
      481 ttggtgtcat tgacatcctt gttaataacg ctggtattat ccgccgcgtt ccaatgtgcg
      541 aaatgagcgc cgctgatttc cgtaaggtca tcgatattga cttaaacgca ccatttatcg
      601 tttcaaaagc agtcattcct tctatgataa agaaagggca tggaaagatt atcaatattt
      661 gctcgatgat gagcgaactg ggacgtgaaa cggttagcgc ttatgctgcc gctaaaggcg
      721 gcttgaaaat gttgacccgc aacattgcgt ctgaatacgg tggagccaat atccaatgta
      781 acggaatcgg accgggttat attgccactc ctcaaacagc acctcttcgt gagttgcaag
      841 aagatggttc tcgccaccca tttgaccagt tcatcattgc aaaaacacct gctgcacgtt
      901 ggggaaatcc tgaagattta atgggccctg ctgtctttct cgctagtgat gccagcgact
      961 ttgtcaatgg ccacatccta tatgtagatg gcggtatctt agcctacatc ggaaaacaac
     1021 ctgagtaaaa atagaaagag gatcttatga aaatcgcatt aatcaatgaa aatagtcaag
     1081 ctagtaagaa tcacattatt tacgatagtc taaaagaagc aacagataaa aaaggctacc
     1141 aattatttaa ctatggtatg cgtggagaag aaggtgaaaa cccattaaca tacgtacaaa
     1201 acggcctaat ggctgccatc cttttaaata caaaagcagt cgactttgtt gttaccggct
     1261 gtggtacggg tgtaggagct atgcttgctt taaacagctt ccctggtgtt gtctgtggtc
     1321 tagcagtgga cccaactgac gcttaccttt attctcaaat caatggtggt aacgccttgt
     1381 ctatccctta tgccaaagga tttggctggg gggcagaact gaccctcaaa ttgatgtttg
     1441 aacgcttatt tgctgaagaa atgggcggtg gctacccaag agaacgtgta atccctgaac
     1501 aacgcaacgc tcgtatctta aacgaggtga aacaaatcac ctacaatgat ttgatgacca
     1561 tccttaaaac aatcgaccaa gacttcctca aagacaccat ctctggcaaa tacttccaag
     1621 aatacttctt tgaaaactgc caagatgatg aagtcgctgc ttatttgaaa gaagtattag
     1681 ccaagtaaag ctattctaaa ccagaaagga actaatggat gacgaaaata ttactgtttg
     1741 gcgaaccatt aattcgaatc tcaccattag atgccaccag tatcggcgat catgttgcca
     1801 gttcgactta ttttggcgga tcagaaatta acatcgcttg taatttgcaa gccctgggta
     1861 tctcaacgaa agtctttacc gcactccctg ccaacgagat tggagatcgt tttctcacat
     1921 tcttgaaaca gcaccaaatc gataccagtt caatctgtcg gcttggcgat cgaatcggcc
     1981 tctacttttt ggagaacggc tttggttgtc gtcaaagtga agttttctac gatcgtaagc
     2041 atacgagtat cagccagatt cggccaaaca tgctagatat gggttctctc tttcagggga
     2101 ttagccattt tcattttagt ggaatcactg tagctatcgg tcaagaggtt cgtacgctcc
     2161 ttctcctact cttggaagaa gccaagcgcc gagggattat cgtttcaatg gacctcaatc
     2221 tgagaacaaa gatgatttca gtcctagaag ccaagtatga attttctaag tttgcacgtt
     2281 tcgctgacta ttgctttggt attgatcctc tcatgattga tgaccaaaat ctagagatgt
     2341 tcccaagaga cagagccagc ctaaaagagg tggaaaatcg catgcgacaa ttaaaagaag
     2401 cctatggttt caaggccatt ttccataccc tccgctctag tgatgaacaa gacaaaaatg
     2461 tctatcaggc ctatgctctg ggagaacgat ttgaagagtc tgtccaacta aaaactgcag
     2521 tctatcaacg agttggtagc ggggatgcct ttatatctgg tgtcctttat caactactcc
     2581 atcattccta cttaaaaact accattgact ttgcagttgc gagcgcaact ctcaaatgta
     2641 ctcttccagg agaccatctc tccacttccg aaactagtat tgaaaaatta ctggcaaatg
     2701 cacaagatat cattcgttag gagaattaca tgaccaaatc agatacgatt attgaactaa
     2761 aaaaacaaaa aattgtcgct gttattcgag gaaatacaaa ggaagaagga ctgcaagcct
     2821 cgattgcttg tatcaagggc ggtatcaaag ctattgaaat cgcctatacc aatcagtatg
     2881 cagggcaaat catcaagcaa cttgtagact tgtatcagga cgatcaaagt gtttgtatcg
     2941 gtgcaggtac tgtgattgat gccgtaactg ctagagatgc tattctagcc ggagcaaatt
     3001 acgttgtttc tccatctttc catgctgaaa ctgcaaaaat gtgcaatctc tacagcacac
     3061 cgtacattcc aggctgtatt actctcacag agataacgac tgcacttgaa gccggtagtg
     3121 aaatcattaa actcttccca ggtagtgctc tcagtccagc atatatctct gcagtcaagg
     3181 caccgatccc acaagtttcc gtaatggtaa ccggaggagt cggcctaaac aacattcctc
     3241 aatggttcgc tgctggtgca gacgccgttg gaattggtgg cgaactcaat aaactcgctt
     3301 cccaaggcga ctttgaccgc atcagcgaga ttgcccaaca gtatgttaca ctcagataaa
     3361 atcataacca cccgtctaag gggtggttta tcccagagct ataagccgtt tctctccagc
     3421 ggcgtctaaa aacgctcgct gaacttcgtt caggtcgtgc tatattactt gactaatgcc
     3481 cgtcacaacg ggcttttacc ttgttctaaa tccgccatca gaaaacggat cttcatattc
     3541 ttttacactc aatttatcta gtgctatagt agattgaaac tggaatagta cacctctact
     3601 tctaaaacat tgttaaaaat cgatttgact gtcctgatcg atttgttctg ttcttatttc
     3661 attttactat atatcatgct ttactcgttc tcaaattttc atacttgtga agaaatcatc
     3721 cactggataa tttctttaat cttgactata tttcttaatt gtggctttat taagccctac
     3781 tgtgctcaca taataacctt ccgcccagaa atgccgattc ccaaacttat acttgagatt
     3841 ggcgtgtttg tcaaacatca tgagtgcact tttgcctttt aaatacccca cacttagcct
     3901 cgacggaata ctgactaaca tgtgtacatg gtctggcatt aagtgaccct cgatcatttc
     3961 aacaccttta taactacaca agcgatgaaa tatttcgcct aaactacttc tatattgatt
     4021 atagatgact tttcgtctat acttaggggt gaacacaata tgatagaaca catccacttt
     4081 gtgtatgata aactatgagc cttttgtgcc atattttttc tcctttcgct ttacaattgg
     4141 attgaacacc tttattgtat cgcgtttgga gtttttttgg tataaccttc gacgcgcacc
     4201 tcacggagcg agacggacta atatagtgga gtgaaatagg atacgaacaa attgattagg
     4261 aaaatcaaat gaatttatag aaatctttta gcagttataa cgtcctattc tagtttcaaa
     4321 acgctatagt cacataatga agtaaaaaag gataagtatc aacttatctt ttttaaaaga
     4381 aaaatccgaa gatatttggc cttcttcgga tttttctatt ttccacagtt tcatgtaatt
     4441 catctagatg atgaacaaat tagttgttct ttcctctacg gaatagataa aacgccccaa
     4501 gtagcaagaa ccctagactt gccaagattg actgaccttc tcctgtctga gggagattct
     4561 tttgatccga atggttcttt tcctcttcag atttttcctt ttcttttgaa ttctgtactt
     4621 gtggctgagt tgcttgctct agctttttaa agacttcctg atctggagct gattcctggg
     4681 tttcaggatt atagtaggca atcttatatt catccccttc ttttcgaatg atatagactc
     4741 cacgtttcaa aacttggaat tggttggaaa tagtagagac agaatcatca tatttcacaa
     4801 tgccccaaac tccttgtttg gcatcataaa cagactgaag ggtttcgtta ttgtcgatga
     4861 ggctactttc taactctttt atcatttgat tgaaggtggc acgatccacg ttagggatga
     4921 gcatatagcc ataagaatct ccattttgct tatgagcctg actaatcgta agaaattcat
     4981 tttcaacttc cttgtctgac tgtccttcat tgatatcctt ccaggctccc ttttgcaaag
     5041 ccttactcat actgattgaa ctcttcttaa agaaaaagta accaatattc tttttcgaat
     5101 cggacgattc taaaaagaca ctttgggttt caggataatc cttttcttgt tctgtaaggg
     5161 aggcttcttt atcattgaca tagactttat atggattacc tgattccagt tttctctggt
     5221 caattgtagt tgcagcagta tctgttgaag tgttttggat attgcttcct aaaaaggcga
     5281 tcttatcctt tagcataaac cagctcttat gagcagtcaa tgtttgattc cagttggtga
     5341 aatccatggt tgctgtcgca ttggcatcat ctagtttgct cgttccaacg aaagcagacg
     5401 gtaaaacttt acctgtatcg ctatccgctc tcttagcatc cgtctctgtt gtaccaggca
     5461 tcttatatgg attgactgtt ggccagtagc catcgctata gtgactcaaa tcgccattgt
     5521 aaagatagaa catcccatca ctcgtatacc aaccacgttt attttccttg ttcatatgtt
     5581 cgtaattcaa ggtacgactg gaaaagagtg acaagccaaa tccaaaccct ttctctgcat
     5641 tgtacatggc tgttttatcc atcttgttaa aggcagatag gtaacttgtt cttggaacac
     5701 ttgcgactcc tgcatcactt aacaaggatt gcatcaaact gatatcctta taagtcttca
     5761 aattcttaaa gacatcataa taactatccg attgaacaat ggtcttcaca agactctgca
     5821 aacgttgttt ggtttctcct tcagacatat ccgctattcg gtgaatccct cttagtactt
     5881 ctactgcggc cacgtgcccc tcgctatttg cacgactgat cgagcgtcca cgactcatat
     5941 ccatcagctc tccattcacc agcaaaggag caaacgattt atcaatccag tggtacatgg
     6001 tttgcatttt atctttatcg attggattct tggtcttttg aatgactggc aacagttgag
     6061 acaggccatc aatcaaaaca ttcccataag cacccgtata ggcaacattg gtatggtcga
     6121 tataggatcc atcttgataa aaaccttcac cttggtctac caacttgaac acttgctcaa
     6181 tcgagcgaat ggtagaagaa atttcttgat catctttacg cagtaaacca gctattactt
     6241 ttactcttcc catatcaact aagtttccac ctagagcctt aaatgggtta tcagtcgtct
     6301 ttcggaaatg ttcgggatct ggtacaaatt tttcaattac atctgtatat tttttaattt
     6361 cctcatcaga gaagtattct ttcatcagag acaaggtatt gttgatggca cgaggtgtac
     6421 caatttcata atcccaccag ttcccaacaa tgctcttttc actattgtag acatgtttat
     6481 gcatccattc catggaatcc ctgactgttc gaacgacagt ttcatcttta taataacgag
     6541 aagaaggatt ggtcacttgc ttggccatct cctccaattt ccgataagtg gcagtcagat
     6601 ttgcagacgt tttataattt gaaaattttt cccacaaata gatgcggtcc gcctgacttg
     6661 aaatactgga taggctatca gctacctttc cttccaattc ctggtttaat ttggccatct
     6721 gttcattttt agaatcatag tattgattcc cagcgatgat gccattccag tcatccaaac
     6781 ggtctgtgta tgcatcctta acagaggcta gaatcttcaa aggaatcttt ttcacttcct
     6841 tgccatcttt actgacaatg acattggttg tcccttcctt aagaggttct aaaattccat
     6901 ttttgactga agcaacgtca ggattttcta ccttataagt atagtccgca agagaaaaaa
     6961 catgtttttt tccaattggt aaatcaatct tttcctcaag ctgtttatct gtttgagaat
     7021 cctcagaagg ctggtctgct acctctacca gctcaatatc cttaaaggaa acagtcccag
     7081 ttcctgtttc atagaataac tccagcttga ttttatcaac atctaaagtc gggctatagt
     7141 ctgcttcaat ggtctgccag tcctttgttc ctgacgtcgt tgcagaattc cacaatcgct
     7201 tgtccttacc actttcctca atgatacgaa ctttggcaat cccgacttta ttatctgttt
     7261 taatcttgaa acgcagttta tactttttct tagcttcaat aggaaccata cggtgaaccg
     7321 ctacccttaa tttctcaggg cttgagatag tgatagcccc atccttagcc tcaatgactc
     7381 gagttgaggc atctgcagaa ctattcttct ggtctaccca agctgaccac ccctgagctt
     7441 tgcttcctgt ccacggactg cttccgtcct ccgtctgacc aaaatcacca ttttcaacta
     7501 aattttttgt atccctttgc tgatactgaa cttccttctg gctttgctga atggtattcg
     7561 tagtcgtttc ttccgctcca actgtcatat aatggtttaa taaaaatcct gataaaacaa
     7621 gtgaagacaa actcacaatg agcttctttg tttttgtttg catgtataca ctccaataaa
     7681 catattgtaa aatcaatagg aactaaatct caaattagag atttatagca actaagtaaa
     7741 ccggtttaat taaatcatac catttttaaa gttatttgta aacggtttct atcatattga
     7801 gggcttattc ttcaacaata tcttccatcc gactcgacga aaaaagtaaa aactgttaca
     7861 atgtagaaaa agaaaagagg ccctcatgac ttcactttat gatttttccg ttttgaacca
     7921 aaacaaccaa gcaactccct tggatagcta tcgtggcaaa gttctcttga ttgtcaacac
     7981 tactactgga tgtggtttaa tgccccagta ccagggactt caagaactct atgaacgcta
     8041 tcaagatcag ggcttcgaaa tattggattt cccttgcaat cagtttatgg gacaagcacc
     8101 cggcagcgca gaggaaatca acgccttctg tagcctacat tttcaaacca ccttcccacg
     8161 ttttgccaag attaaggtca acggtaagga agcagaccct ctctatgtct ggttaaaaga
     8221 ccataaatct ggcccactag gaaaacgaat cgaatggaat ttcgctaagt ttctcattag
     8281 tcgtgatggg caagtctttg aacgcttttc ttcaaaaaca gacccaaaac aaattgaaga
     8341 ggcgatacaa actctactat aattcacaat ctcactatga ttaggtttcc tttaacctga
     8401 tgaatagtga gattttttga ttggctttga cttaaataga aaaacacccc atgatatgaa
     8461 acatgaagtg ttgtaaagtc tatgttgtag gtgcttattt cacattttca atgtgaccag
     8521 tgataacgaa taccatacag aatcttcata tacactaaac aaatgacttt ctaattatat
     8581 caattagttt tggctagtaa atatcatttc caacaaacgc cctctcaatt ccttatcctg
     8641 atgatgcaag atattcatta agtcatgaga gtttttcgca ttgatgaatt gatttaacaa
     8701 tctatctttt aattcatatg gaagagaagc tgtctttagt agtctaaaaa cttcgtcatt
     8761 taaagatgtc cttttattat ctttccattc aaatttagct gtatcattct tatttggcaa
     8821 ttcaattata gacacattcg ttcctttaaa atgaattcta tgttttctat tgcttggaac
     8881 gatactagaa tctccttgta atgctaactc taccattccc atttcccaat cgattgataa
     8941 tcttgtttta tatctttgac cattttgatc ttcaagcatt tcaaaataat gttgttttcc
     9001 tgggaataca taccaatcta caacttcagg taaatcaaca cccatatcta tctcagaacc
     9061 aaccaaggga atgattgcac cactttttgc aaacacaggc gtagtcgaga tgtccctata
     9121 aacacttaac ttcacaccac ctgtgtattt tttctctgaa aaaaagtcat accattcacc
     9181 ttcagggaac catacatcta cttttgcaga ttggaatgtc aaatccatct tttctacaat
     9241 gggagccacc atcagttctg ttccaaaaaa gtattggttt ggaacattat agctctcatc
     9301 attctctgga tagaaataat agattggact gattaatggg gcaccttcct catgtgtctg
     9361 tacattcatg gtatatagat agggaatcat ctgatgtctc aaacgaaggt atttcttcat
     9421 aatcttagat gttgtttctg aaaaaaacca aggttcttta ctattaaaag gacttctaga
     9481 actatgtaat cgagtaatcg gactaaaaac accaaactgt agccatctag tttgtagctc
     9541 ttcgtcataa tcccccaaca tatgtccacc gatatcatga ctccaccaac tataaccgat
     9601 attagatgct gtcgctgtaa aatagggttg aaatcttaag gaattccaac taataatagt
     9661 atcccctgaa aaaccaacag ggtagcggtg actaccagga cctgcatatc ttgataaaat
     9721 caaaccacct tctgcatttt tacaactatc ctgatagtga taatggttta aaagccaaag
     9781 tggatctagc atgccttgtg tcccctgttg ccagtcaatc caccaaaaat ctactccctg
     9841 cttttctagc tcataatgaa catctttaaa gtaggcttcc ctaaaagagg gattaaaaaa
     9901 atcaaaaata gcaggttctt ctagttctac atttaacccc aatcgttttg cgatttgagg
     9961 ataagcttct tcataagccc gtatcccatc agcaggatgg acatttaagg agagttttaa
    10021 ctttctatca tgaagttgtt gcaataactg ttctggattt ggtattaagt ttctattcca
    10081 actatatcct gtccagccac ttccaaaacg agctggaatg tcagttatat gccaatccat
    10141 atctaacaca ccgatagata atggaatttt ctctgtttca aatctgtcta ttaaatccaa
    10201 gtattcatcc gacgtataag gccaatatct actccaccaa ttgcctaaag catatcttgg
    10261 caacaagggt attgaaccag tcaaatggta aaagtctctg attgctcctc tataatcatg
    10321 cccataggca aagaaataca ggtcaatttg attttctctc tcaatataac cagattgttc
    10381 atcccaaata aatccttgag aatcatccaa taaggctata ccatttcggc taataattcc
    10441 atcttctaac gagattgctc catctgcctt atccagagtc cgagctgttc cttttaacgt
    10501 ttcaatagat tcaccaaaat accagcgact accatatacg gcaaaatttc cttttaattc
    10561 tataaataaa ttttcggcgt taaattctcc tttattaaag tgcagatgaa aatagtccgt
    10621 cataatatct agtacatttg atgtctcgat ataatctaac gaaacttggc caaaatctct
    10681 attatagata agttgtgtcg ttctatcctc aaaatttcca gttcgagagt attctaacct
    10741 tactagcttg tctgttaata cagagattcg ataaaactct cccttaaaaa ttttcaattt
    10801 gttttcctcc ttttatggta gcataaaaac agaacgcacc atttttgatg cgtttttcat
    10861 tattctgaat gcaatgttct a
//
LOCUS       DAVHIU010000061        10699 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVHIU010000061 DAVHIU010000000
VERSION     DAVHIU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.063"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(207..1649)
                     /gene="gtfA"
                     /locus_tag="VVN06_001332"
     CDS             complement(207..1649)
                     /gene="gtfA"
                     /locus_tag="VVN06_001332"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU5562874.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSVFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNHSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(1926..2006)
                     /locus_tag="VVN06_001333"
                     /pseudo
     CDS             complement(1926..2006)
                     /locus_tag="VVN06_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2007..2840)
                     /locus_tag="VVN06_001334"
     CDS             complement(2007..2840)
                     /locus_tag="VVN06_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5562875.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(2854..3720)
                     /locus_tag="VVN06_001335"
     CDS             complement(2854..3720)
                     /locus_tag="VVN06_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5562876.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(3734..4993)
                     /locus_tag="VVN06_001336"
     CDS             complement(3734..4993)
                     /locus_tag="VVN06_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5562877.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(5085..7247)
                     /locus_tag="VVN06_001337"
     CDS             complement(5085..7247)
                     /locus_tag="VVN06_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU5562878.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            7355..8215
                     /locus_tag="VVN06_001338"
     CDS             7355..8215
                     /locus_tag="VVN06_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5562879.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKKGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            8212..9147
                     /locus_tag="VVN06_001339"
     CDS             8212..9147
                     /locus_tag="VVN06_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU5562880.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(9273..9360)
                     /locus_tag="VVN06_001340"
     tRNA            complement(9273..9360)
                     /locus_tag="VVN06_001340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9324..9326),aa:Ser,seq:gct)
     gene            complement(9367..9440)
                     /locus_tag="VVN06_001341"
     tRNA            complement(9367..9440)
                     /locus_tag="VVN06_001341"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9404..9406),aa:Ile,seq:gat)
     gene            complement(9476..9546)
                     /locus_tag="VVN06_001342"
     tRNA            complement(9476..9546)
                     /locus_tag="VVN06_001342"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9512..9514),aa:Gly,seq:tcc)
     gene            complement(9566..9638)
                     /locus_tag="VVN06_001343"
     tRNA            complement(9566..9638)
                     /locus_tag="VVN06_001343"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9603..9605),aa:Phe,seq:gaa)
     gene            complement(9642..9715)
                     /locus_tag="VVN06_001344"
     tRNA            complement(9642..9715)
                     /locus_tag="VVN06_001344"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9679..9681),aa:Met,seq:cat)
     gene            complement(9726..9815)
                     /locus_tag="VVN06_001345"
     tRNA            complement(9726..9815)
                     /locus_tag="VVN06_001345"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9777..9779),aa:Ser,seq:tga)
     gene            complement(9826..9899)
                     /locus_tag="VVN06_001346"
     tRNA            complement(9826..9899)
                     /locus_tag="VVN06_001346"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9863..9865),aa:Ile,seq:cat)
     gene            complement(9913..9986)
                     /locus_tag="VVN06_001347"
     tRNA            complement(9913..9986)
                     /locus_tag="VVN06_001347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9950..9952),aa:Met,seq:cat)
     gene            complement(9992..10065)
                     /locus_tag="VVN06_001348"
     tRNA            complement(9992..10065)
                     /locus_tag="VVN06_001348"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10029..10031),aa:Pro,seq:tgg)
     gene            complement(10081..10154)
                     /locus_tag="VVN06_001349"
     tRNA            complement(10081..10154)
                     /locus_tag="VVN06_001349"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10118..10120),aa:Arg,seq:acg)
     gene            complement(10164..10249)
                     /locus_tag="VVN06_001350"
     tRNA            complement(10164..10249)
                     /locus_tag="VVN06_001350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10213..10215),aa:Leu,seq:taa)
     gene            complement(10257..10328)
                     /locus_tag="VVN06_001351"
     tRNA            complement(10257..10328)
                     /locus_tag="VVN06_001351"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10294..10296),aa:Gly,seq:gcc)
     gene            complement(10351..10423)
                     /locus_tag="VVN06_001352"
     tRNA            complement(10351..10423)
                     /locus_tag="VVN06_001352"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10388..10390),aa:Thr,seq:tgt)
     gene            complement(10442..10523)
                     /locus_tag="VVN06_001353"
     tRNA            complement(10442..10523)
                     /locus_tag="VVN06_001353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10487..10489),aa:Leu,seq:tag)
     gene            complement(10529..10601)
                     /locus_tag="VVN06_001354"
     tRNA            complement(10529..10601)
                     /locus_tag="VVN06_001354"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10566..10568),aa:Lys,seq:ttt)
     gene            complement(10626..10698)
                     /locus_tag="VVN06_001355"
     tRNA            complement(10626..10698)
                     /locus_tag="VVN06_001355"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10663..10665),aa:Asp,seq:gtc)
BASE COUNT         3308 a         2373 c         1931 g         3087 t
ORIGIN      
        1 aattcccaat aaaatcagct ctttactaga caaccccttc agcttttcaa taaagcctat
       61 tgatttcaaa atgaatcctt tctctccaaa tcagctgatt cagataatag taagctatcc
      121 tatattgtac cactttttta gcaatttgaa aacaaaggaa acgttttcca aaataaaaac
      181 cctattttat ccaccatatc aaggcttcaa aatgatactt caactccatt ctcaattacc
      241 cgataagtct gattttgcaa atcaatttct actactgctg ttacggactt atctttattt
      301 tgacgtttga ttacaatgct gtgagctgtt ggtgtctcta tctcagtagt cccttctaga
      361 tcaaaggctt ctgaatggtt acggaaagaa aatagattga gaagggcctt cacaacaggt
      421 cgttgcactt cttttgctat ttcctcgttg ctatagtaat gacgattaat atttcgacct
      481 tctttagttt cttctaataa tttcaagtca ttcttgcctg ctaatagacc cacatagtaa
      541 acctgaggaa tacctggggc aaaagcttga attagacgag cgagaaaata cttgacatca
      601 tcatctccaa gcgctgaata gtaggttgaa ttgatttggt agatatctaa gttgttatac
      661 tcggcactag agtacttacg tttgacattg gctccaacct tatagagttc atttgaagca
      721 tagtcaatct cctcatcggt caggatatcc ttgacatcta ctactccaat cccatcatgg
      781 gtatctagcg tcgtaaattg cttcatcggg ctcatcttta accacttagc caaacgctct
      841 gttctggaac tgtaaagagt ataaagtgtc accattggaa gagcaaaatc ataaacatag
      901 taatcatggt ctgctatttt aaactgaatc gaatagtgtt catgaatctc aggtaaaagc
      961 tctgtcccat actcagcagc gatatctcga actttgtcca ataaatccca aatatctggt
     1021 tccacaaaga aatcattagt atccaatttc ttcactgcat aagcaaaggc atctagacga
     1081 atcaaatcac acccattact tgccaagtgc tgaatggtct tacggataaa ttccatagtt
     1141 acttctttgg tcacatcaag atcaatctgc tcctcaccaa aggtattcca caaatgttcc
     1201 actgaaccat cttcaaacac aatctcttgc tttggtgcac gatccttacg cttgtaaatt
     1261 aaatctacat cagactgtgt cggacggttt tctggccaaa acttatccca gtttaaaaag
     1321 agagctttaa attcactggc ttcatgtttt tcttgatagt ccttataata cttggattga
     1381 cgagaaatat gattaatcat aaaatcaaac ataagataat atttctcacc taaacgctta
     1441 acatcctccc aatcaccaaa aactgagtcc acttcgtcgt agtcaactgg cgcaaatcca
     1501 cgatcacctg ttgatgggaa aaatggtaaa aggtgaactc ctccaatagc atctccaaaa
     1561 tgctcttcca aattatcata taagtcttta agattatttc caaggctatc agaataggta
     1621 atcaacatgg ttttattttg aatcggcatc attactctcc tttttctaat tgaagccaag
     1681 tctcatatga tctggcttcg taaatcaaat tcattttaaa tctctattta tcatcaaact
     1741 cgtactaata tagactgtga taaacaaagt actactttct tgttttctac atagaattat
     1801 caacaagctg aactcttcct ctgtgtcaaa gactatagat tccatgagct cttcttatac
     1861 tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttac cgtaggtatg gttactgact
     1921 tcgtcagttt catccacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
     1981 gtttgctctt tgattttcat tgagtattac ttcactgccc cgttgctcat tcctgaaatg
     2041 atatggcgtt ggaagaagag atagacaatg gtgatactga taatgccgac cacgtaagag
     2101 gcaaagcttg gtccgtagtc gttgaaatat tggcctgcgt agttgtattg gaacaaaggc
     2161 agagtccaca ttttggaatc ccggttcaag acaaggagtg gcaacatgaa gtcattccag
     2221 aaccaaaggg cattgatgat catggttgtc gcatgcatcg gtttcatcat tgggaagatg
     2281 atgcggaaat aggttgtaaa ttgattagcc ccatcgatct ctgctgcttc atccagactt
     2341 tctggaatcg agattttgat atagccaaca tagagaaaga gggtctgtgg aatcgcatag
     2401 gtcaagtaga gtaagatcaa accaaaggta ttagccaaac cgagtttact catcataacc
     2461 gtaatcggaa tcatgatgac ttggaaaggt acgaagattc cgaggattaa gagggtatac
     2521 atgatggtaa aggcttttct tttactcata ttgcgagcga tggagtaggc tgccataggt
     2581 ataaagatca ttactgcaag taaagacaag acagtgatga cgacagagtt ccaataatag
     2641 cctccaatcc catcagctaa gagacggcta aagttgtccc atgtgaagtt ggttggaaag
     2701 ccaaagaaat tatctacaat atccttagtg ggtttgaagg aactaaagag ggtagcaagg
     2761 agcggcacta aaatcagaac cgatcctaga atcaatagaa tgtatttgcc aatcagggct
     2821 tttctttcat cttgtttcat catgcttctc ctcttaaatt tcaaatttct tagatactct
     2881 caattggatg atcgaaatca ctacaattaa gaagaacaag attacggcaa tggcattggc
     2941 ataaccgaat tggttgtttt taaaggcata gttataaacc aagagcccaa gtgaggttgt
     3001 ggcattgttt ggaccaccac cggtcatggc aaagacttgg tcaaaggcag tcagcccacc
     3061 ttttagggct aggataaaga ccatagagac acttggtagc aagtaaggca attcaatgtt
     3121 ccagaaaact tgcttgctcg tcgcaccatc aatccttgct gcctctgtaa tctcagttgg
     3181 aatagattgc aaaccagcta ggaagatgat gatgggcata gccacccctt gccaaagaag
     3241 gacaaagaca gccgcaaaga ttgctcccca cttagtccct aaaagactgg tttggagaaa
     3301 ttcaatatga agggcattcc caatcgctgg aagaccgtag ttgaagactt gcttgaagat
     3361 caaagccact gtcaaaccag ataaaacagc tgggaagaag aaccaagcac ggaagaaggt
     3421 ttggcctttg attttagaat tcaagacacg cgcaatgaag atcccgagtg caatctcacc
     3481 aaccaccatg gcaatcgtaa tgattgcggt aaagccaatc gcattcatga attttggatc
     3541 catgaagagg agcttaaagt tgtttaagcc gacaaatttg tagttataag tcaatcctgt
     3601 ccagttggta aaactgtaaa aggctccttg aaacatcggc acatagaaga aaattgcttg
     3661 taacaagagg gggatgacca caaaagccca tgcccaatat ttttgtaata cttttttcat
     3721 agtctctcta ctcctaatcc acatccgctt tcatcgggtt aaagaaggca ttcaaatcat
     3781 tgaccatgcc ttgtttatca ccggtcaaga catagttcat ggtcaaggta tggaagtctg
     3841 cttcactggt ccagtattgt tgcaaccaga ccaagtgacg atccgtaaag gcatattcgg
     3901 tcataccagc aagcggtgaa tcttctcctg cttgtttgac tccttcgatc gctgttggag
     3961 atccgtccac atcgtagtat ttttgcatga cttctggacg ggtcatatat tccacaaagg
     4021 cattggcttc ttttggatgt ttggtggtgg ctgagataga ccatgccaag tctcccgcac
     4081 caacggttaa gctttgtcct ttttcttttc ctggaatcat gaaggtccca atcttaaagt
     4141 tcggtttttg ttcattaatc gctgtgatcg cccaagaccc atttggtgtc atgaggacat
     4201 ccccacgtgc gaaggctccg ataacatcgg tatagccagc accttcccag ttcttttgct
     4261 tagagccatt gatgcgaagg atgtccatga ccttgatatc atctttcata atcggatccg
     4321 acaatttaat ggcatttggt tgagaataac gaaggtattg atttgcttct tttcctccac
     4381 ctgttgctgt cgcaaaggct aattgattgt aaccattgag tgtccaagca tctgcacctg
     4441 caattccaaa tggtgtttgt cctttagcaa cgatatcttt gactagctgt tcaaattcat
     4501 cccaggtttc aggaaccttc aagcccagtt cttcgaattt atctttgttg tagtaaattc
     4561 cataagcatt agctgtaaaa ggaacgttgt aaactttttc gtttacagca tatttttcag
     4621 cgtagccatt tttcacgcgt ttcaggtagt ctttgttgct caaatcttca aaaacacctg
     4681 cttttgccca ttcttgcagt tcgatggact gtgggtaaat attgaccaca tcaggcacat
     4741 ctcctgcgag aacgcgtgtc ttcaatactt caccagcatt tggtacattg acgactttga
     4801 ccttgatctt agggttttcc ttctcaaaat cacgagtgat ttcttccaag gttttggtca
     4861 tttctttttt ctggttgaaa tactcgatgg tcactgtgcc atccgcagat ttaccatagt
     4921 tggagcaagc gccgagccca aacaaagcta aacctgtagt tgcaagaagt ccgatttttt
     4981 tataccattc cattagaaag cctcctttat aaatttatac acccttattg aactgcaccc
     5041 caaaagttag acagaataaa tctaactttt ggggtcagta catatcatag ttttctaaaa
     5101 atatactgtc tactcaaaaa atctccttgg gataagataa cagttaagcc cgcatacatt
     5161 agttctgcac ctgagtaaac ttcgccattt tcctgtaatt tatatagtcc ctcttcatcc
     5221 aaatctttta attttaaagt tgtttccatg gtctctacaa cagataaaac gcgaacgtag
     5281 gttacaatcg tttgatttcc gtaattaaat tgtacagctg cttcattgga tacagtatca
     5341 ggattaatta gtctatactg ctgtcctaac tgaactactg gtcgtaattc tttatacaag
     5401 ttcacctgat tagcaatcgt agctttctct tcatctgata aatttgtcaa atcaagctca
     5461 tagcccaaat ttcccatcat tgctacatgg ccacgtgttt ctaatggtgt cattcgtccc
     5521 atctgatgat tcggtactgc tgacacatga gcccccatag aaatggttgg atagagatag
     5581 gatgaaccgt attgaattgg taaacgtgca atggcatcag tattatcact agcccagact
     5641 tgtgggaaat agcgtatcat accaagatca tttcgtccac caccaccaga gcaggactca
     5701 aagagaatat ggctgtgctt ctctgtcaga taagaaacga gttcgtaaag ccccagcatg
     5761 tactgatgag attgcatctg tgtctctaga taagttaatc cattccctag cttagtgata
     5821 ttgcggttca tatcccattt aatgtaatca atctcatgat aagataggag ttgatctaag
     5881 acacttttca agtattctac tacctgagga ttggcaagat taagtactaa ttgattccga
     5941 gaataagtat gctcataatc aggaacctga atagcccagt caggatgttg acgatacaaa
     6001 tcactatcta cagaaatcat ttcgggttct aaccaaagtc caaactgcaa acctctttca
     6061 tggatagctg aaatcagact ttctagactt ccacccagtt tttcctcatt aacaacccaa
     6121 tcacctaaag cacgattatc atcaaaacga ttgccaaacc aaccatcatc taatacaaaa
     6181 agttcaatgc caactttctt agcttcatct gctaactcta acagtttttc tctctgaaag
     6241 tcaaagtaag tagcttccca gttattgatt agaattggac gttctttttt agaaaattca
     6301 cttagcataa tgtgcttcag tacaaaattc tgactttcat gactaatacc agttaatccc
     6361 tgatctgaat gagtcactaa agctaccggt gtttcaaagt attcctcagg ggctaacttc
     6421 caagaaaagt tttctggatt aatgccaata gccacccgaa cttcattcaa ttgatttttt
     6481 tgaacaaaag cttcaaagtt gccactatac attagttgaa tagcaaacac attcccagca
     6541 tcctctgtga ctccttgttc gcatagtaga agagctggtg tttgagcatg accagaagca
     6601 cctcggtttg agctaatcga aaagattcct tgttctacct gttgacgtct aacagtcttt
     6661 tcacgagcat aagcaccctg cagagttact atttcgtaat ctgcagctgg aaaatcagcc
     6721 ataaaagaaa aatctttatg gatgacaact tcctgattac tattattatc taatttactg
     6781 tagctagcaa tagtcgcatc attattaaaa gtagtataat acaaagtcag actaagttga
     6841 gccttagaat cttctaacat taagacaaga gtctctgtat cgtccatgct atgtggagaa
     6901 ggtaagccct gtggaccatt ctgacctttt aaaatctttg cttctacaaa tcgaaagtct
     6961 gttacttcag ttacactatg ctgaacctgt atggttggtt tcctaaaatc tcctaagcca
     7021 tgttgtccaa aaatctgtcg ctgagtatct aaactaaagg ttcgattagt agccgttgga
     7081 tttcctgaaa aggcatggtc tcgttcataa acactattgg aacctttata gttcttaata
     7141 gtctttccta aatgtttcaa aagtaagtag ccatttcgat tttcaataat caaacttaga
     7201 tttttactct caacataaaa tagattattc tctatcctaa ctcccattta cttcacctca
     7261 tcactttatt gattatattt tatcacctga aatcgctttc cgaaatagaa aaatgtctca
     7321 agaatatggt aaaatgttag gtaggaggta gcacatgtta gttttttcag aataccagac
     7381 tggaacaatc gaccttgccc taagctttta tggatatgag gaatgcacac ctaattactc
     7441 ttttggtcca gccattcgtg atacatacgt tctacattac attactaaag gacaaggaaa
     7501 attttattac aagggtaaaa ttgttgattt aaaaaaagga gatttctttc tattaaaacc
     7561 agaggaacta accttttatc aagcagatag taaagaacct tgggcctact actggttagg
     7621 aatcactgga gggaaatccc ctgattattt tgctctttct caaatttctg atcaatccta
     7681 tctcatccaa tctgaaactt gtcataccca gactactgca aaactcatct cagacattgt
     7741 ccgcttcgct cagattacaa aatcaagtga attagctcaa ctccatatca tgggacaact
     7801 tcatgaactg atgtttcatc tgggaactat tgctcccaat cagaaaaaaa agaatatttc
     7861 atcaacccac caactctatc ttgaatgcaa acgattaatt gatagccact atcctcaatc
     7921 acttacaatt caagatttag caaaagaact atccgttcac agaagctact tatcaagcgt
     7981 attcaaagaa tttaatacct tatcacccaa agaataccta ctctacgttc gaatgcaccg
     8041 agctagacaa cttctcgaaa atacccaaga gtccatcaag gtaattgcat actcggtagg
     8101 tttttcagat ccactccatt tttcgaaagc ttataaacaa tactttaatc agactccaag
     8161 tcatacaaga aaagaatact ctcaatacca actagtaaga aaggcaacat tatgaaatcc
     8221 taccaagctg tctaccaaat cctatctaaa gaaaccgact atatcagcgg agaaaaaatc
     8281 gcagaaaaac tatccctaag ccgaacagca atttggaaag ccatcaagcg actagaacaa
     8341 gaaggcattg aaattgatag tatcaaaaat agaggatata aactgatgaa tggtgacctt
     8401 attcttccag agattctaga agaaaatctt ccaattaaag tcagctttaa acccgaaaca
     8461 aaatcaacac aactagatgc aaaagaagca attgatttag gccatgaagc aaataccctc
     8521 tatctagctt cctatcaaac agcaggccga ggccgttttc aacgttcctt ctactcacca
     8581 caaggtggta tttatatgac actccatctt aaaccaaatc tcccctatga caaattacca
     8641 tcctacacac tacttgtagc tggagctgtc tacaaagcta ttaagaacct aactttaata
     8701 gatgtcgaca taaaatgggt caatgatatc tatctaaaca atcataaaat tggaggaatc
     8761 cttactgaag caatgacctc tgtagaaact ggcttagtca cagatatcat tattggagta
     8821 ggtatcaatt tcactattaa agacttccct caggaattaa aagaaaaagc tgccagctta
     8881 tttaaagcta cagctcctat aacaaggaat gaattgatca tagaaatctg gcgtgctttc
     8941 ttcgaaacac cagcagaaga gctattatac ctatacaaaa aacagtcatt cattctagga
     9001 aaagaagtca ctttcacact agagcaaaaa gactacaagg gacttgctaa agacatctca
     9061 gaaaatggaa aacttttaat tcaatgtgat aacggaaaag aaatctggct aaatagtggc
     9121 gaaatttctc tcaatagttg gaagtaaaat aacacaatta taatataaac gatataaaaa
     9181 taacttcaga ttagtaattc aattaagttt tacggatctg aagttttatt gtctctaaaa
     9241 acaaaaaaga gagttacaaa ctctcattaa aacggagaat aagggattcg aacccttgcg
     9301 ccagttaccc gacctaacga tttagcaaac cgtcctcttc agcctcttga gtaattctcc
     9361 aattaatggg cacgagtgga ctcgaaccac cgacctcacg cttatcaggc gtgcgctcta
     9421 accacctgag ctacgcgccc aagttaaaaa acttggtaat ttgaacaaag ttcaaagcgg
     9481 gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tagttttacc actaaactac
     9541 acccgcataa atactatcaa taaaatggcg cgagacggaa tcgaaccgcc gacacatgga
     9601 gcttcaatcc attgctctac caactgagct accgagcctt attgcgggag caggatttga
     9661 acctacgacc ttcgggttat gagcccgacg agctaccgag ctgctccatc ccgcgttaat
     9721 ataaaaggag gatgtgggat tcgaacccac gcacgctttt acacgcctga cggttttcaa
     9781 gaccgttccc ttcagccgga cttgggtaat cctccaatat tcaaatggac cttgtaggac
     9841 ttgaacctac gaccactcgg ttatgagccg agagctctaa ccagctgagc taaaggtccg
     9901 acaagatcat tatagcggcg aaggggatcg aacccccgac ctcccgggta tgaaccggac
     9961 gctctagcca gctgagctac accgccatga atcgggaaga caggattcga acctgcgaca
    10021 ccttggtccc aaaccaagta ctctaccaag ctgagctact tcccgagtta aatagaaaaa
    10081 tgcaccctag aggagtcgaa cctctaaccg cctgattcgt agtcaggtac tctatccagt
    10141 tgagctaagg gtgctccata ttatgccgag gaccggaatc gaaccggtac gatcgttacc
    10201 aatcgcagga ttttaagtcc tgtgcgtctg ccagttccgc caccccggcc tctctaagcg
    10261 aacgacggga ttcgaacccg cgacccccac cttggcaagg tggtgttcta ccactgaact
    10321 acgttcgcac tgttttcttc tatctaaaaa tgccggctac atgacttgaa cacgcgaccc
    10381 tctgattaca aatcagatgc tctaccaact gagctaagcc ggctcatttg ttatatctta
    10441 atgcgggtta agggacttga acccccacgc cgttaagcgc cagatcctaa atctggtgcg
    10501 tctgccaatt ccgccaaacc cgcatatatg acccgtactg ggctcgaacc agtgacccat
    10561 tgattaaaag tcaattgctc taccaactga gctaacgagt ctaaaataac ttgcgttacc
    10621 ttaaacggtc ccgacgggaa tcgaacccgc gatcttcgcc gtgacagggc gacgtgataa
    10681 ccgctacact acgggacct
//
LOCUS       DAVHIU010000062        10495 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVHIU010000062 DAVHIU010000000
VERSION     DAVHIU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.064"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(22..1446)
                     /locus_tag="VVN06_001356"
     CDS             complement(22..1446)
                     /locus_tag="VVN06_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5562881.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(1990..4269)
                     /gene="pulA"
                     /locus_tag="VVN06_001357"
     CDS             complement(1990..4269)
                     /gene="pulA"
                     /locus_tag="VVN06_001357"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU5562882.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHFLVVVNAKSDPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4381..6339)
                     /gene="ligA"
                     /locus_tag="VVN06_001358"
     CDS             complement(4381..6339)
                     /gene="ligA"
                     /locus_tag="VVN06_001358"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5562883.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6432..7610)
                     /locus_tag="VVN06_001359"
     CDS             complement(6432..7610)
                     /locus_tag="VVN06_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5562884.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7690..8553)
                     /locus_tag="VVN06_001360"
     CDS             complement(7690..8553)
                     /locus_tag="VVN06_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU5562885.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLDHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8556..10457)
                     /locus_tag="VVN06_001361"
     CDS             complement(8556..10457)
                     /locus_tag="VVN06_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5562886.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3136 a         2336 c         1892 g         3131 t
ORIGIN      
        1 acaatttcct gattttacac gttatttcac atcaaaaaca atggatttga catttgtcat
       61 cgcttcaatg ctatatttaa ttccttgcac tccagctcca gaacctttga caccaaggaa
      121 tgggaaatta tctggaccac gctgggtttt attattaatg tggactgtac ctacttcaag
      181 tttttcagca atttcaaagg cttttttgaa atcatttgta aaaactgatg attgaaggcc
      241 gaattcagat tcgttggcaa aggcaatagc ctcctctaca ctagccacac gaatgattgg
      301 taaaacagga ccaaatggct cttcccatgc cactttcata tcttttgtaa cttggtcaaa
      361 aagcactggc cagaggagat tgccctcacg tttgattggt gtaagagcct gagcctcttt
      421 ttcttgtgca tcctcaatca agccccaaat gaagtcggct gaagcattgt caataacagg
      481 tgtaatatca gcattgtcaa atggatcacc aactgttaat ttagaaactt cttcctgaag
      541 caaagtagct aatttatctg ctacactttc gagaacaatg acacgtttaa tggccgtgca
      601 acgttgtcct gagtagctaa aggctcccgc aacaatttgc ttggcagcat gttccaaatc
      661 tgcatcttct agtacaagag ctgcatcttt cccaccaagt tccaacatga taggacgcat
      721 accagctaaa cgaccaatac gttctccaat aggagttgaa cctgtaaagt tgatgaagtt
      781 gacttctttg tgctcaatga tataatcccc aatttctgaa ccacgacctg taatggtgtt
      841 gaaaacacct gccggaatcc ctgcttcttc aaatgcttta gccaacaaga gtccagaaat
      901 ggaaccttgt gttggtggct taaacatgac cacattccct gcaatcaagg caggtgcaat
      961 tttagaagca gataaattaa ctggataatt aaaaggagca atcgctagca cgataccaac
     1021 tggttcacga cggacaacag ccagtttgtt tttacttgct gcctcaaaac caccaccttc
     1081 cattgcttgt ccagtgatac ggagaccttc ctcagcagca taacgaatca agtctgctgt
     1141 acgcactact tctccaattg ctgctttaat cccttttgct acttctttgg caaggatagt
     1201 accaattttt tccttatcgc gttctaaaat agctgctgtt ttatgcaaat aagccgcacg
     1261 ttcaattgct gataaagctc gccatgctgg cagggctgca cgcgcagctt gcatagcctc
     1321 atcagcttca gtctgagtca tggctggaac tgtacccaat tcttcttgat tgattggtga
     1381 gtaaatcgta atttcttgtt cagatgattt ccattttcca tttactaaat tctgatatct
     1441 tgtcaaattc ttgtcctcct gaaaatgatt aagatatatt ttaccaaatt ttcagaaaat
     1501 cacaaccctt tttgcaaaat ttttgagatt attttcacaa acttgatttt tcaaagtata
     1561 ctcaataaaa ataaaaaaaa tccactacgt caaggcgagg ctaatgtggt ttgaagaaat
     1621 tttcgaagag cgtgaatgag tatcatctat agtaaaataa aaaactgaac aatttggttg
     1681 gggacagcca aaccaatttc tcacaatgtt tcagaaacaa gggtgtgcta ctccaatttc
     1741 ggcctactat aactgtcata gattgctgaa acaaagtcta ggtaaaagtc ttcataataa
     1801 aaagacctcc tatcaaatgt tcaaaaactt tgataggagg tcttgtttcg tgaaaatatt
     1861 tatcaaattt tctatacaag tgagctgtta gccaggttct ttctattctt tcaatttcaa
     1921 tgaatggatt ttttaccaat actcataact gggaattttt ctgtctaaaa atagcgagat
     1981 agatggtatt tataaaacac tcaagacagc tagactaata tcatttaaaa cattatcttc
     2041 ttttgagcga ctgttggtta ccaacatagc taaatttcct gcattttcaa attgataggg
     2101 gtctgattta gcattcacaa ccaccaagaa gtgttctttg ccgtgaactt catagataag
     2161 gtagccgcta tgttcaatcg cagaatgcac aaagacatga tggtaaattt catcatagct
     2221 agagtaagaa aaggcaccag tttttgtctt caatcggatg acttgacgga taaactcaat
     2281 actgtcttga cgctcattaa tcaagttcca gttcacttgg ttcacactgt caggagcatt
     2341 atagctattc atcgcacgct ctctatcatc atgggttaac tcaccatttt caccagtcgc
     2401 aaccagtttg gtacgaccaa attcttgacc gatttccata aaggccatcc cctgcatgag
     2461 cagattcatg gctgtggcag tttcgacctt gcgcatgatt tgctctgaac tttggtctgg
     2521 atgaagggtt gccaataaat cgtgaagatt gtaattgtca tgggcttcta catagttaag
     2581 cacctgattt ggatgtgtat agcttcctaa ttcacgactt cctaggattg ctttagctag
     2641 aattggctct gtcgcagcac cactgacaaa acctgacttg atagcaccat aaacttctcc
     2701 ccctttgaca gcatcgcgct gattgtcatt aaagaaacca atatttggca tctggtaggc
     2761 attgtccttc ttggccttat cataaggggc aagacctgtt cccatatccc atccttctcc
     2821 atagaggata atgttggagt cgatttcatc caagctttga cgaatcatct gcatggtctt
     2881 gacatcatga atccccatca agtcaaaacg gaagccgtca atattatatt cctgcaccca
     2941 gtatagaaga gaatcaatca tatacttgcg aaacatttcg tgttcactgg ctgtttcatt
     3001 tccaacaccc gttccattct ggaaggtacc atctggattc atacgataat agtaatcagg
     3061 gactgttgtt tggaatggtg catcaacaac tgagaaggta tggttataga ctacatccat
     3121 aatgactcca atacccgcat cgtgataagc ttgaaccatc accttcaaat cacgaatgac
     3181 ctgagctgga tcatctggat tagttgaaaa actagtttct ggcgcgttat agttttgtgg
     3241 atcataaccc cagttgtagg ttacatttcc atcctcatcg tattctttat gacggtctgc
     3301 aattggttgc aattgaacat aattgtagcc cagcttcttg atgtaatcaa aagcagttga
     3361 ctggccgtat tggttaactg ttccagcctg agcagcaccc aagaaagttc ctcgaagatg
     3421 ttcatctaca cccgatgtag gtgatttagt caaatcacga atgtgcattt cacagataac
     3481 tgccttacat ggattttcca agcgccaagt agcctccgaa ccgtgcttaa cctcgaagtt
     3541 ttcaacttgc ttttctacat ggctcagaat agctgaacgt ttgccatcag ggctggtcgc
     3601 gattgtataa ggatcacgtg tcagtgtttg gtgatgaggg aattggactt gatactgata
     3661 agtcttacct accaaatctt cttcaacatc caaactccag acaccgattg tattgtcctt
     3721 atgattataa gagtagctat tgcctctttt catctcaaaa gtcttccaaa cgggtgcatc
     3781 attagcagct gattcataaa cgacaacttg cacttctgtc gctgtaggtg accagagaga
     3841 aaaatgagcc tgattgtcct ctacacggca acccaattct ccttggtaac cccaatgatg
     3901 atcaaaacta gcactgttaa tggccttatc aaaggcaaaa ggattttgat ttttatagaa
     3961 aggactggca atagcaggat tttcagagta ataaatccta tcatcgcctt ccaaaatcca
     4021 gacctctgtt aataggggat agtgattaaa acggatagaa tattctttac tagtttgacc
     4081 tgtatgaacc acaaaattca agctttctat aacatgtgaa cttgggtgtt caaagctaaa
     4141 taaagctcca aaataatctt ctttgtaggt tagcaaatca attcgttgat cctgactttt
     4201 tacaaaggag caagtgtcat attctccatt cttacgatgg taatgaatgc gcatagggta
     4261 gttatacatt ttttattttt cctttttact ttgtttctat ttcactaata aatttttgtc
     4321 aatctcgtct caattaacag acatagtcat attctctaaa ctctgttttt aaacgatcca
     4381 ttacaaactt tctagccatg cctcatctct gacctggata ccaagttctt gtgctttttg
     4441 cagtttactt ccagcgtctg cacctaccac gacgaggtcg gtctttttag aaacactacc
     4501 tgtcactttg gcacccagac tttcgagttt acttttagct tctgagcgct tgagtcgttc
     4561 caattttcct gtcaatacca cggtcaaacc tgacaaggcc gcatccgcta ctaccgtctg
     4621 tcctttatag tccagattga ccccagtttc tttcaattct ctgagcagaa tttcagagcc
     4681 ttctgtcgca aaataagtct gaagactttt ggcaatcacg ccacctagac tttcaatact
     4741 agccacttcc tctgaatctg cctgatacag attttcaatt gaatggaaat attgaagtaa
     4801 aagctgacta gccttgcttc cgacatgacg aattcccaaa ccaaataaga gcttctcggc
     4861 agaattttcc tttgatgctt ggatagcctg atacagttta gcagcggact tttccttaac
     4921 cccctctaaa aggaggaaat cctcttcttg caaacgataa atatccgcca catccttgac
     4981 taaattagca gcaaaaagct tctcaacaat agatggacca aggcctgtaa tattcatagc
     5041 atcacgagaa gcaaagtgaa tcaagccttc catgatttga gcagggcaac gcggattgat
     5101 acaacgtagg gccacttcat cttcaaagtg caacaagtca gagttacaac ttggacagtt
     5161 tgtagggata tctagttttt cttcagaaac ccgtttggac tctaccacac gtaaaacggc
     5221 agggatgatg tcaccagcct tatatacaat gaccgtatcg tcttttcgga tatctttttc
     5281 agcaatataa tctacattgt gcagggtcgc acggctaaca gtcgtaccgg caagttgtac
     5341 tggtgttaga ttagcagttg gagttacaac accggtacgg ccaactgtcc agtcaactga
     5401 taagagttga gcttcttttt cttcagcagg gaacttgtag gctactgccc actttggagc
     5461 cttaacggta aaaccaagtt cttcttgact tgctaggtcg ttgaccttga ttaccactcc
     5521 atcaatatcg taaggcagat tttcccgttc ctgtcctact tcttggataa aattccagat
     5581 ttcatctatg ttttcagcca agattcgctt aggattgacc acaaaaccta gttgttctag
     5641 gtacttcaaa cccttttctt ggctatcacg agttgaaggg ctggcttctt gatagagaaa
     5701 cgttgcaaga ttacgcttgg caactactgc tgtatccaac tgacgcagag ttcctgctgc
     5761 cgcattacga ggattagcaa attcaggctc tccattttct tggcgcactt ggttaacttg
     5821 gtcaaaggaa gcgcgtggca tgtaacattc cccacgaact gtgatatcta gttcttctgg
     5881 caaagtcaaa gggatgtcct taacacgctt gaggttttct gtgatatttt caccaattga
     5941 accatctcca cgtgttaccc cagcaaccaa aatccccttt tcataagtca gcgagataga
     6001 taagccatcg attttcagct cacaaatata ggtcggatga gccacttcct tacgaacacg
     6061 cgcatcaaaa gcatctagct cctcacgtga aaaagcatcc tgcaaactat aaagaggata
     6121 ctgatgactg tatttttcaa aaccatctaa aaccttgcca ccaacacgat gagtcggact
     6181 gtctgctagc acttgctctg gataagcagt ttctaactcg accaactcac ggtaaaggcg
     6241 gtcatactca ctgtctgaaa ccgagggatt atcgctggta tagtactcag tcgcatagcg
     6301 attgagcaaa gcgactaact cattcattct tttattcata agaccatttt accataaaac
     6361 aagccctcct cacaaacgag aaggacggaa aaaacactta gtttgaaatt atttttgaaa
     6421 ctcaagcaac cttatatcaa tttttcaaaa tgagttcgaa cataaataaa cgatatacaa
     6481 gacaagatga taacaccact tccaattatc aggaaagaag agagatgtac acttggcaag
     6541 actgtcataa atccttttgc aataggcata aatagaatag ctaaggtaaa aattgtactc
     6601 agtactctcc caagaaattc gctctcaacc ttggtttgta cttgagtaaa aaagtgaata
     6661 ttaaaaatcg tcataaacaa ttcacaaact aaatttccag aaaaggaaag aaaagttgga
     6721 agtggtaatc ccatcataaa aactccgaca cctgtcaaag ccagtaaaat caaaagatta
     6781 taaatattag ctttaatttt actagctaga agagccccaa tgatggaacc aatagccccc
     6841 atagttaaaa tacttgcata ggctccttct gacccgtaaa gctgattcga aaagggaagt
     6901 agaaattcaa aagctgcaaa aaagaaatta acgctggaag ctaccagcaa aaggaagaaa
     6961 atttcttgct gatgccagat atagtgtaac ccatccttga tatctacaaa aatatctctc
     7021 ccagtaaaag cctttttctc ttgaactttt gcttcctctt ttggaaggaa agccactaga
     7081 acaaaagcaa tgaaaaaagt cagcgagtct agcagtagcg tcatatggag acttgcaaac
     7141 tgtaaaacaa ggaaggaaag aacaggagag ctaacaccta caacctgcaa aaccagctct
     7201 aggcgagaat tatagatcac aatctcatct ttctccacca cttcagttat gatagcttta
     7261 ttggctgtgc gagaaaaggc aaaagcaata gcctgcacaa tgttagcaac aatcaaagcg
     7321 ccaatcatcc agctatcatt ccttatgaaa gaaatagcca gacaaagaat cccacaaaca
     7381 agatctgccg tcattaaaat cttacgacga gaaaaacggt ctgaaataac tccgccaaag
     7441 ggattgacga gaatagatgt gacgagctca gaaatctgat acattcctaa aactgtctgt
     7501 cctatagtcc ccatggaagc caaccagaca ctatttccat aatcatagag catatttccc
     7561 attttattga tagccccacg gctaatcaac tgcactgcat agcgattcat attaaagctc
     7621 ctctcaaatt ttgaaactat tgtatcaaaa ccgaaaggag ctttttattt tttcccttat
     7681 ttgggaaaat taacttttga caaatttttc gtagtgttcc tgataatagg ctacttgctc
     7741 tggaagacct aacacatcaa aaatatgcat ggcctcttgc atctgcttac agccttcttt
     7801 acactgtcct ttttgatata aggcaaaacc ttttaaataa tggaaaacat tacgctcata
     7861 aagcttaata cctttgtcaa taatcttctc tgtataagcc tcaaaatagt tggcattata
     7921 aaaagaagaa tgatctaaac aatgctggta acaattgagg gccaaaatca acactaatct
     7981 cttatggcga ctaatctctt ggtaaaattc ctccctctcc ataacttctc taccaatccg
     8041 agtgacatag tctacatcgt agaaactata gaggttaccg aaaagaatca actcatacat
     8101 ggtccattct tctgttttga agagataatc tgctacctta tccaaatcat cctgcttcat
     8161 atcataactc gcatctcttt gacaaatcag accttgtagc aaaatccagt tcagctcaaa
     8221 ataaagggga gtcgtcgaac tcttagactt ttcaagttgt tctctttgaa gcttttgaaa
     8281 acctgcaata tcgtttgaat agtaaagtgg gataatctgt gccatcatag acacatgttc
     8341 atgattatga aaattccttg ccttatccat gaaattttcg attgttacat gaatgttatc
     8401 caaaatctca aagaaacggg agactgccag gtcagactcc ccaagctcaa agcgagataa
     8461 ctgagaggta gagcaggatt cgcctgctgc ttcctttaaa gaataatttc cacttgttcg
     8521 aaattcacga aatacttttc caagatgttc catctttaca cctgatctga taattcttcc
     8581 cactcaagca tagcttcttc ctgacgatgg ctgattttgt ccagctcagc ctgtaattcc
     8641 atgagtttgt cggcatcgtt tgtttccaac atttgttcag aaatggcttg gctttgactt
     8701 tctagctctt caatttcagc ttctagactt tcgatttgtc gcatgagttt gcgaacttct
     8761 ttttgacttt ctttctgggc ctgatagtca ttgactggac ttgcttcctt tgcttgattg
     8821 ctagttgaag cttcctcagt ctgactcatt tctgctgttg ctttcttctc aacatagtag
     8881 tcgtaatctc caaggtagag agttgaacca ttctcagaca attccaaaac atgagttgcc
     8941 acacgattga taaagtaacg atcatgactg acaaaaagca aggtaccatc aaagtcaatc
     9001 aaggcatttt ctagcacttc cttactatca atatccaagt ggttggtcgg ctcatccaga
     9061 atcaaaaagt tattgttttc catagacaat ttagctaaaa gcaaacgagc tttttcgcca
     9121 ccagatagca tgccgactga ttttttaaca tcatctcctg agaaaaggaa ggctccaaga
     9181 cggttgcgga tttcaacttc tggtgtcagt ttgaaatcat tccagagttc atccagcacc
     9241 gtattacttg gtgtcagctt gctttgggtt tggtcatagt aacccacctc aacattagcg
     9301 ccaaagcgct tttctccctt gataaaagga atctggtcca caatagactt gataaaggtt
     9361 gacttgccga taccatttgg accaacgata gcgacagcat tcatcttacg aagatctagg
     9421 ttgataggtt gtgacaagac ttccccgtca taaccaatag cagtattttc aacagtcaaa
     9481 acaacattgc ccgacgtttt ttcagactgg aaggtcatgt tggctgcttt cttgccagct
     9541 tcaggcttgt ccaaacgctc cattttttct agttgtttac ggcgagattg agcacgttta
     9601 gtcgttgaag cacgaactag attgcgattg acaaagtctt ccagagcagc gatttccttc
     9661 tgttgctttt catagttttt tgcctcagta gctagctttt gctccttcaa ttcgacaaaa
     9721 cgagagtaat tccccacata gcgatccaag gaatgcttgg tcaaatctag cgtaattgtc
     9781 gcaaccttgt ccaagaaata acggtcgtgg ctgacgataa ggagggcacc gctatagttt
     9841 accaagtaat tctctagcca ggcgatggtt tcaatatcca agtggttagt tggctcgtcc
     9901 aagaccaaga gattgggctt ttcaaggagc attttggcaa gtgccaaacg agtattttga
     9961 ccaccagaaa gctcagcaat tttcatctgc cacatagact cgtcaaactt gaatccattc
    10021 aaaatcgctc gaatatcagc ttcataggta aagccacctg cttggcgaaa attctcagat
    10081 aagcggtcat aatctgacat cagtttatcc aaatcctcac cagacttttc acccatctcc
    10141 agctccatct gacgcagttg tctctccgtc cgacgcaaat tattaaagac atgaagcatt
    10201 tcatcgtaga tggtattttc agactcaaaa cggctatctt gggctaggta agacagagaa
    10261 atatcttttt tcttattgat ttctccgcta gttggctcct cttctccaac taaaatcttc
    10321 aaaagagtag acttacctgc accatttttc ccaacaaggg caatccgatc tcgttcatca
    10381 acctgcaggt tgatattatc gaaaagaacc tctcctgcaa aagaacgttc aattttatta
    10441 gcttgtaaaa taatcataag agtagtatag catgtttccc taaggcattc aagat
//
LOCUS       DAVHIU010000063        10091 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVHIU010000063 DAVHIU010000000
VERSION     DAVHIU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.065"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            68..199
                     /locus_tag="VVN06_001362"
     CDS             68..199
                     /locus_tag="VVN06_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU5562887.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            220..1770
                     /gene="dltA"
                     /locus_tag="VVN06_001363"
     CDS             220..1770
                     /gene="dltA"
                     /locus_tag="VVN06_001363"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU5562888.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            1767..3011
                     /gene="dltB"
                     /locus_tag="VVN06_001364"
     CDS             1767..3011
                     /gene="dltB"
                     /locus_tag="VVN06_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU5562889.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            3025..3264
                     /gene="dltC"
                     /locus_tag="VVN06_001365"
     CDS             3025..3264
                     /gene="dltC"
                     /locus_tag="VVN06_001365"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU5562890.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            3257..4525
                     /gene="dltD"
                     /locus_tag="VVN06_001366"
     CDS             3257..4525
                     /gene="dltD"
                     /locus_tag="VVN06_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU5562891.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            4680..5120
                     /gene="adcR"
                     /locus_tag="VVN06_001367"
     CDS             4680..5120
                     /gene="adcR"
                     /locus_tag="VVN06_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU5562892.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            5120..5824
                     /locus_tag="VVN06_001368"
     CDS             5120..5824
                     /locus_tag="VVN06_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU5562893.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            5817..6623
                     /locus_tag="VVN06_001369"
     CDS             5817..6623
                     /locus_tag="VVN06_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU5562894.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            6633..8138
                     /gene="adcA"
                     /locus_tag="VVN06_001370"
     CDS             6633..8138
                     /gene="adcA"
                     /locus_tag="VVN06_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU5562895.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(9309..10082)
                     /locus_tag="VVN06_001371"
     CDS             complement(9309..10082)
                     /locus_tag="VVN06_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5562896.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
BASE COUNT         2926 a         1956 c         2157 g         3052 t
ORIGIN      
        1 gaaaaataag ctagtaagaa tcactcttat tttagcgaaa aattacagaa atgaatggtt
       61 tttcttgatg aaacagagaa aagaattgta cctctttctt ggtcggacag ccttgtattt
      121 tcttatcttt ctagggctgc tttacttctt tagctatctt ggtcagggtc aaggaagctt
      181 tatctataat gaattttaac ttgaaggaga atccctattg tgtcaaataa accaatagca
      241 gatatgattg aaaccattga gcattttgct cagacacagc ctagctatcc tgtctataat
      301 gttttggggc aggaacacac ttatggcgat ttaaaggctg attcggatag tttggctgca
      361 gtcattgacc aactaggctt gcctgagaag tctcctgtgg ttgtttttgg tggccaagaa
      421 tatgaaatgt tggcaacctt tgtagcgctg actaagtcag gtcatgccta cattccaatt
      481 gatagccatt cggccttgga gcgagtttca gctattttag aagtagcaga gccaagcttg
      541 attattgcca tttcagcctt tcccttggag caggtttcta caccaatgat aaatctagct
      601 caggttcaag aagcctttgc ccaagggaac aactatgaaa tcacgcatcc agtcaaggga
      661 gatgataatt actacattat ctttacttct ggtacgactg gtaagcctaa gggagtgcag
      721 atttcacatg ataatctcct cagctttaca aactggatga ttacggataa ggaatttgcg
      781 acaccgagtc gtccgcaaat gctggcacag ccaccttatt cttttgactt gtctgtcatg
      841 tattgggcac cgaccttggc acttggtggt acgcttttca ctcttccttc agtcatcact
      901 caggacttta agcaactctt tgcggctatc ttttcattgc caatcgctat ctggacatca
      961 acaccatcct ttgcagatat ggccatgttg tctgaatact tcaacagtga gaaaatgcct
     1021 ggaatcacgc atttctactt tgatggtgaa gaattgacgg tcaaaacagc tcaaaaactg
     1081 cgcgagcgtt tcccaaatgc ccgtatcatc aatgcttacg gcccaacaga agcgacagta
     1141 gctctgtcag cagttgccgt gacagacgag atgttagcga ctctcaaacg cctaccaatc
     1201 ggctatacca aggctgattc tccaaccttt atcattgacg aggaaggaaa taaactgcca
     1261 aatggcgaac agggagaaat cattgtttct gggccagctg tttcaaaagg ttatatgaaa
     1321 aatcctgaaa aaacagcaga agccttcttt gagtttgaag gtctgccagc ctatcacaca
     1381 ggcgatgtgg gaaccatgac agatgagggc ttgcttctct acggcggacg catggacttc
     1441 cagattaagt ttaacggtta ccgcattgag ttagaagatg tctctcaaaa cctcaacaag
     1501 tctcgcttta tcgaatctgc tgtcgcagta ccgcgctata acaaggacca caaggtacaa
     1561 aatctattgg cttatgtcat cttaaaagac ggtgttcgtg agcagtttga gcgagatatc
     1621 gatattacca aggccatcaa ggaagacctg acagacatca tgatgtccta tatgatgcca
     1681 tctaaattcc tttaccgaga cagtttgcca ctaactccaa atggaaagat tgacatcaaa
     1741 ggattgatta acgaggtgaa taagagatga tggagttttt tcaacagctt cctcatttag
     1801 agccatatgg caatcctcag tattttgttt atgtgattgc tgcaaccttg cccatcttta
     1861 taggtctctt tttcaagaaa cgctttgcct ggtatgaagt gttggtaagt ctcttcttta
     1921 ttgtcaccat gttggtgggt ggaaagacca atcaactagc tgccttgggt atttaccttt
     1981 gctgggaaat attgctcctg cttttctaca agcattatcg aaaaagcaag gatggcaagt
     2041 gggtcttcta cttagttagt tttctgtccc tacttccgat tatctttgtc aaggtgcaac
     2101 cagctatcaa tggaacgcag tctttgcttg ggttcttggg aatttcttac ctgacctttc
     2161 gttcggttgg gattatcatc gagctgagag atggagtgat taaggatttt accctctggg
     2221 aattcctccg tttccttctc ttcatgccaa ctttctcgag tggtccaatc gatcgcttta
     2281 agcgatttaa tgaaaattat caggctattc ctgagcgaga tgagttgatg gatatgctgg
     2341 atgaatctgt ccgctatatc atgtggggct ttttgtataa gtttatccta gctcatgttt
     2401 taggagagac cttactacct cctctgaaga atttagcctt gcagtcaggt ggcttcttta
     2461 atctctatgc cttggcagtt atgtatactt ttggtctgga gctcttcttt gactttgcag
     2521 gttattctat gtttgctttg gccatctcaa acttgatggg aatccgtagc cctatcaact
     2581 ttaacaagcc ctttttatca agggatttaa aggagttttg gaatcgctgg catatgagtc
     2641 tgtccttctg gttccgtgac tttgtcttta tgcgaatggt gatggtgtta accagaaaga
     2701 aagtctttaa aaatcgtaat gtaacctcaa gcatggccta cattgtaaat atgctgatta
     2761 tgggattttg gcatggtgtg acctggtact atatcgccta tggactcttt catggactag
     2821 gcttggtcat caatgatgcc tgggttcgca agaaaaaaac gctcaataag gaacggaaaa
     2881 aagcagggaa ggctgcccta cctgagaatc gctggattca gttgcttggc atggttgtca
     2941 ctttccatgt tgtcatgttg tcattcttaa tcttttctgg attcttgaat aatctatggt
     3001 ttaaaaaata aaaggaaata aaatatggat atcaaatcag aagttattga aattattgat
     3061 gagttgttta tggaagatgt ttctgacatg atggatgaag atctttttga tgcaggtgtc
     3121 ttggatagta tgggaacggt tgagttgatt gtggagattg agaaccgttt tgacattcgt
     3181 gtccctgtaa cagagtttgg tcgtgacgac tggaatacag ctaataaaat catagctggt
     3241 attgtggagc tacaaaatgc ttaaacgctt atggatgatc ttcggaccgg tcttgatcgc
     3301 tggtttgttg gtttttctgc tcattttctt ttatcctact gagatgcatc ataatctagg
     3361 agctgaaaag cgttcagcag tggctactac tatcgatagt tttaaggagc gaagtcaaaa
     3421 agtcagagca ctatctgatc caaatgtgcg ttttgttccc ttctttggct ctagtgaatg
     3481 gcttcgtttt gatggtgctc atcctgcggt attagctgag aaatacaatc gttcctaccg
     3541 tccttatctt ttaggacagg ggggagctgc atcgcttaac caatattttg gaatgcaaca
     3601 gatgttacca cagctggaga ataaacaagt tgtgtatgtt atctcacctc agtggttcag
     3661 taaaaatggc tatgatccag cagccttcca gcagtatttt aatggagacc agttgactag
     3721 ttttctgaaa catcaatctg gggatcaggc tagtcaatat gcagcgactc gcttactgca
     3781 acagttccca aacgtagcta tgaaggacct ggttcaaaag ttggcaagta aaggagaatt
     3841 gtcgacagca gacaatgaaa tgattgaatt attggctcgt tttaatgaac gccaagcctc
     3901 cttttttggt cagttttcgg ttagaggcta tgttaactac gataagcatg tagctaagta
     3961 tttaaaaatc ttgccagacc agttttctta tcaagcaata gaagatgttg tcaaagcaga
     4021 tgctgaaaaa aatacttcca ataatgagat gggaatggaa aattatttct ataatgagca
     4081 gatcaagaag gatttgaaga aattaaagga ttctcagaaa agctttacct atctcaagtc
     4141 gccagagtat aatgacttgc agttggtttt aacacagttt tctaaatcta aggtaaaccc
     4201 gatttttatc attccacctg ttaataaaaa atggatggac tatgctggtc tacgagagga
     4261 tatgtaccaa caaacggtgc agaagattcg ctaccagtta gaaagtcaag gttttaccaa
     4321 tatagcagat ttttctaagg acggcgggga gcctttcttt atgaaggaca ccattcacct
     4381 tggttggttg ggttggttgg cttttgacaa ggcagttgat cctttcctat ccaatcccac
     4441 accagctccg acttaccatc tgaatgagcg ctttttcagc aaagattggg cgacttatga
     4501 tggagatgtc aaagaatttc aatagataat ataatataga agagttcaag aatgaaacta
     4561 gttttcacgt tttttcttga ctcttttttt gcttgtgata taattaactg gtaaacaaaa
     4621 tgtcaaagaa tagtattttt ctcttaaaaa agagaagtct aaaaggaaag gagtcagata
     4681 tgagacagct agcaaaggat atcaatgctt ttttgaatga ggtgattttg caggcggaaa
     4741 atcagcatga aatcctaata ggtcattgca ctagcgaggt ggccctgacc aatactcagg
     4801 agcatatcct tatgctcttg tcagaggaat ctttaacaaa ttcagaattg gcccgtcgtc
     4861 tcaatgtcag tcaggcggca gttaccaagg ccattaagtc tttggtcaag gaagggatgt
     4921 tggaaacatc taaagattct aaagatgcgc gtgtgatttt ttatcagttg actgacttgg
     4981 ctcgtccaat cgctgaggag caccaccatc accatgagca tacactttta acctatgaac
     5041 aagtggcgac tcagtttact ccaaatgaac aaaaagtgat tcagcggttt ttgactgctt
     5101 tagtaggaga aatcaaataa tgagatatat tacggtagag gatttgtcct tctattatga
     5161 taaggagcct gttcttgaac atatcaatta tagtgttgat agtggggaat ttgttacctt
     5221 gactggggaa aatggagcgg ctaagacgac gctcatcaag gccagtcttg gaattctgca
     5281 accacgcatt ggaaaggtgg ctatttcaaa gacaaatacg caaggtaaga aattgagaat
     5341 agcctatctt cctcaacaaa ttgccagttt taatgctggt tttccaagta cggtctatga
     5401 atttgtcaag tcgggtcgtt atccgagaaa aggctggttc cgtcgtttga atgctcatga
     5461 tgaggagcat atcaaggcca gtctggactc agttggcatg tgggaacatc gagacaaacg
     5521 cttgggttct ctatctgggg gacaaaagca gcgagcggtg attgcgcgta tgtttgcttc
     5581 tgaccctgat gtgtttatcc tagacgagcc gacaacgggg atggatgcag gaagtaaaaa
     5641 tgaattttac gaactcatgc atcacagtgc ccatcatcat ggcaaggctg ttttgatgat
     5701 tacccatgac cctgaagaag ttaaggatta tgcggatcgc aatattcatc tagtccgtaa
     5761 ccaagactcg ccatggcgtt gtttcaatgt tcatgagaat ggccaggagg tgggccatgc
     5821 ttagtttatt atcttacgac tttatacaac gcgccttttt ggcggttatt gctatgagtc
     5881 ttttctcacc ggtattggga accttcctca tcttgcgtcg tcagagtttg atgagtgata
     5941 cccttagcca cgtctcactt tcaggtgtag cctttggtct ggttttgggg atttctccaa
     6001 ctgtttctac tattgccatt gtcttgattg cggcggtctt tctggagtat ctccgtacgg
     6061 tttacaagag ctttatggaa atcgggacag ctatcctcat gtcaacaggt ctggctgttt
     6121 ctctgattgt catgagcaag ggtaaaagct cgagttcaat gagtttggac caatatctct
     6181 ttggttcgat cgtgactatc agtgaagaac aggtcatttc cctctttgtc attgcggcgg
     6241 ttgttttgat tttgaccttt ctctttcttc gtcctatgta tatcttaact tttgacgaag
     6301 atacggcctt tgtggatggc ttgccagttc gtaccatgtc cattcttttt aacatggtga
     6361 caggggtggc tattgccctt atgattcctg cagcaggagc tcttctggta tcgaccatta
     6421 tggtcttgcc agctagtatt gccctgcgtc tggggaaaaa ctttaaatcg gttatgctgc
     6481 ttgccagtgc gattggcttt ttgggaatgg tagcaggact ttacatttcc tactatgcag
     6541 aaacacctgc aagtgcaagt attaccatta tttttgtaac tgtctttata ctaatcagtt
     6601 tagtaagacg ttttatcaaa taggagacga acgtgaaaaa aattagctta ttactagcca
     6661 gtctatgtgc cttgttttta gtggcttgtt ccaatcaaaa acaggcagat ggtaaactca
     6721 atatcgtgac aaccttttac cctgtctatg aatttaccaa gcaagtcgca ggagatacgg
     6781 ctaatgtaga actcctaatc ggtgctggga cagaacctca tgaatacgaa ccatctgcca
     6841 aggcagttgc caaaatccaa gatgcagata ccttcgttta tgaaaatgaa aacatggaaa
     6901 catgggtacc taaattgcta gataccttgg ataagaaaaa agtgaaaacc atcaaggcga
     6961 caggcgatat gttgctcttg ccaggtggcg aggaagaaga gggagaccat gaccatggag
     7021 aagaaggtca tcaccatgag tttgaccccc atgtttggtt atcaccagtt cgtgccatta
     7081 aactagtaga gcacatccgc gacagcttgt cagcagatta tcctgataaa aaagagacct
     7141 ttgagaagaa tgcagctgcc tatatcgaaa aattgcaagc cttggataag gcttacacag
     7201 aaggcttgtc tcaagcaaaa caaaagagct ttgtgactca acacgcagcc tttaactatc
     7261 ttgccttgga ctatggactc aaacaagtcg caatctcagg cctttctcca gatgcagagc
     7321 catcagctgc tcgcttggca gaattgacag agtatgtcaa gaaaaataaa atcgcctata
     7381 tctattttga agaaaatgcc tcacaagccc ttgctaacac actttcaaaa gaagcaggtg
     7441 tcaaaactga tgtcctcaat cctttagaaa gtctgacaga agaggacacc aaggctggag
     7501 aaaactacat ttccgtgatg gagaaaaacc tcaaggcttt gaaacaaaca acagaccaag
     7561 aaggcccagc aatcgaacct gaaaaggcag aggataccaa gacagtccaa aatggttact
     7621 tcgaggatgc agctgtcaag gaccgcacct tgagtgacta tgcaggtaac tggcaatcag
     7681 tttatccttt ccttgaagac ggcacgtttg accaagtctt tgactacaag gctaagttga
     7741 ctggtaagat gacccaggct gagtacaagg cttactatac aaaaggctat cagacagatg
     7801 tgactaagat taacattact gataatacta tggaatttgt tcaaggtgga caaagcaaga
     7861 aatacactta caagtatgtc ggtaagaaaa ttttgactta caagaaaggc aatcgtggcg
     7921 tgcgtttcct atttgaagcc acagatgctg acgctggaca attcaagtat gttcagttta
     7981 gtgaccacaa tatcgcccca gttaaggcag aacatttcca tatcttcttt ggaggcacaa
     8041 gccaagaagc cctctttgaa gaaatggaca actggccaac ctactaccca gataacctat
     8101 ctggccaaga aatcgcccaa gaaatgttgg cgcattgatg agaaatcgac tagttcataa
     8161 gagctagtcg gttttttgat actagatggc taacttcatt atagaccttt cagaactaag
     8221 ggaacctgaa aaaataaata aaactcttga aatatctggg cttatatgct agaatgtagt
     8281 tagtgtataa ggttcattag aacaccaaag cccctaacta gtgccatagt taagggcttt
     8341 tttgacgtat cttagcgatt taacgggtgt ttataggcta gtcagtcggt ctacttctta
     8401 ccatctagcc atttgcagac gaaatacaag ataattccag ctataacatc tgctataata
     8461 gtaagagcaa gctctggtat aaggctcatt agtttcacct cctctcacga acccatagga
     8521 acgtaatcgg taaccgatga caaaagtatt ataccacact aaatttagat catcaacctc
     8581 acttaatgct tgaactattg tagattatat gctaaaatgt agttaattga taaggttcat
     8641 tagaacacca aagcccctaa ctatggccgt agttaagggc ttttttgacg tatcttagcg
     8701 atttaacggg tgtttatagg ctagtcagtc ggtctactcc ttactatcta gccatctgca
     8761 gacgaaatac aagataattc cagctataac atccgctata atagtaagag caagctctat
     8821 gataaggctc attagtttca cctcctctca cgaacccata ggaacgtaat cggtaaccga
     8881 tgacaaaaat agtataccac aatacattta gatcatcaag gtcgcttaat tcttgaaata
     8941 tagggtctag gcgacaaagt ctttaaaatc agaaaaacgc ataatatcag gtgtgcaaaa
     9001 acttgatact atgcgtttta ttgtgggaag gtttactcca ttttctcctg aaattgagtt
     9061 tttgtccagt ctctgttttt agggttgcta agaaaataat gtcatgtggt gaatatttgt
     9121 aaatcagtca gcagacagaa cgaatactct tcgaaaatct tttcacatca tgtcagcttc
     9181 gtctttccgt atatatgtga ctgacttcat cagttctatc tacaacctca aaacagtgtt
     9241 tcgagctgac ttcatcaatt ttcaaatctg tactttgagc aagctgagac tagcttccta
     9301 tttgattttc attgaatatc agaaacccat tctccatcaa ataattcgac tgcgtctaat
     9361 aatttttggt ctggcaaggt gtcagaaata aaggttgtgt atttggatag gggattaatt
     9421 ttaaaaaatc cagtcttgta aaatttagaa ctatcaatca gtaagatggt ttcatgggct
     9481 ttgtcaataa tattcttttt tgaaatagct tggctgagag aagcttcata aacatattgg
     9541 tcatcaatac ctcttgctga acaaaatgct aaatcgatat taaaatgatc taataaagaa
     9601 ttttccttat catagttgac cacggaacag gattgatgtt tgacctcgcc agatgtgata
     9661 aagattttgg agctatcttt aacagtttca gatagggttt gtgcagtatg taaaccattt
     9721 gtaaaaataa tcaaattatc aagttcagaa agatagggac aaagttcgta gacagtagta
     9781 ctagaatcta gatagataca cataccagac cgaataaagt ctttagcgag actagcgatt
     9841 agtctttttt gcctagtact ttctccttca cgtatttgat gagaaagttc aattgtgttc
     9901 atagaggaca gggtcacgta tccgtgcttt cttttgataa gaccttgatt ttctaagaaa
     9961 attaaatcac gacgtaaggt acttgtgctg gagaaagtga tttctgccag ctcttttacg
    10021 gcaattcttt ttttcttttt gataatttca atcaattcaa gtacacgttc atcttttatc
    10081 ataagctcct c
//
LOCUS       DAVHIU010000064         9724 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVHIU010000064 DAVHIU010000000
VERSION     DAVHIU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.066"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            44..6058
                     /locus_tag="VVN06_001372"
     CDS             44..6058
                     /locus_tag="VVN06_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5562897.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLVELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTKLINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKKLELRNVSDIELYSQTNGTYR
                     QHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNGQSVYKITAKAEKLQQEL
                     ENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRYAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPFILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIAEVKAKQKQAGTKYSIGVYDRITSNTWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEAKFDSSNPAMKNFFGPVG
                     NKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVLDPTERFQNAADLQNYVH
                     NMFDLIYMMEYLEGQSIVNKLSVYQKIEALRKIENQYLTDPADGNDVYATNVVKNLTE
                     DEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSGEKGTPGDLMG
                     RRIAFELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGKERGLVTDELVLKKVFDG
                     KYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPRYGTKTINNVDELQKLMDEAVL
                     QDATGTRWSNYNPETDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6110..6286
                     /locus_tag="VVN06_001373"
     CDS             6110..6286
                     /locus_tag="VVN06_001373"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5562898.1"
                     /translation="MQAGIPPDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            6506..7402
                     /gene="ylqF"
                     /locus_tag="VVN06_001374"
     CDS             6506..7402
                     /gene="ylqF"
                     /locus_tag="VVN06_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU5562899.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDRYIGRPRWQRLKKSKNSL"
     gene            7366..8145
                     /locus_tag="VVN06_001375"
     CDS             7366..8145
                     /locus_tag="VVN06_001375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5562900.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8161..9711
                     /locus_tag="VVN06_001376"
     CDS             8161..9711
                     /locus_tag="VVN06_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5562901.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGAICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3300 a         1833 c         2005 g         2586 t
ORIGIN      
        1 aaggtctttg taacatatat aaattttatt tcaaggagga ataatggaaa agtattttgg
       61 tgaaaaacaa gagcgttttt catttagaaa attatcagta ggacttgtat ctgcaacgat
      121 ttcaagttta ttttttatgt ctgtattagc tagttcatct gtggatgctc aagaaactgc
      181 gggagttcac tataaatatg tggcagattc agagctatca tcagaagaaa agaagcagct
      241 tgtctatgat attccgacat acgtggagaa tgatgatgaa acttattatc ttgtttataa
      301 gttaaattct caaaatcaac tggtggaatt accaaatact ggaagcaaga atgagaggca
      361 agccctagtt gctggtgcta gcttagctgc tctgggaatt ttaatttttg ctgtttccaa
      421 gaaaaaggtt aagaataaaa cggtattaca tttagtattg gttgcgggaa tgggaaatgg
      481 tgtcttagtt tcagtccatg ctttagaaaa tcatcttttg ctaaattaca atacggacta
      541 tgaattgacc tctggagaaa aattacctct tcctaaagag atttcaggtt acacttatat
      601 tggatatatc aaagagggaa aaacgacttc tgattttgaa gtaagtaatc aagaaaaatc
      661 agcagccact cctacaaaac aacaaaaggt ggattataat gttacaccaa attttgtaga
      721 ccatccatca acagtacaag ctattcagga acaaacacct gtttcttcaa ctaagccgac
      781 agaagttcaa gtagttgaaa aacctttctc tactaaatta atcaatccaa gaaaagaaga
      841 gaaacaatct tcagattctc aagaacaatt agccgaacat aagaatctag aaacgaagaa
      901 agaggagaag atttctccaa aggaaaagac tggggtaaat acattaaatc cacaggatga
      961 agttttatca ggtcaattga acaaacctga actcttatat cgtgaggaaa ctatagagac
     1021 aaaaatagat tttcaagaag aaattcaaga aaatcctgat ttagctgaag gaactgtaag
     1081 agtaaaacaa gaaggtaaat taggtaagaa agttgaaatc gtcagaatat tctctgtaaa
     1141 caaggaagaa gtttcgcgag aaattgtttc aacttcaacg actgcgccta gtccaagaat
     1201 agtcgaaaaa ggtactaaaa aaactcaagt tataaaggaa caacctgaga ctggtgtaga
     1261 acataaggac gtacagtctg gagctattgt tgaacccgca attcagcctg agttgcccga
     1321 agctgtagta agtgataaag gcgaaccaga agttcaaccg acattacccg aagcagttgt
     1381 gaccgacaaa ggtgagactg aggttcaacc agagtcgcca gatactgtgg taagtgataa
     1441 aggtgaacca gagcaggtag ccccattgcc agaatataag ggtaatattg agcaagtaaa
     1501 acctgaaact ccggttgaga agaccaaaga acaaggtcca gaaaaaactg aagaagttcc
     1561 agtaaaacca acagaagaaa caccagtaaa tccaaatgaa ggtactacag aaggaacctc
     1621 aattcaagaa gcagaaaatc cagttcaacc tgcagaagaa tcaacaacga attcagagaa
     1681 agtatcacca gatacatcta gcgaaaatac tggggaagtg tccagtaatc ctagtgattc
     1741 gacaacctca gttggagaat caaataaacc agaacataat gactctaaaa atgaaaattc
     1801 agaaaaaact gtagaagaag ttccagtaaa tccaaatgaa ggcacagtag aaggtacctc
     1861 aaatcaagaa acagaaaaac cagttcaacc tgcagaagaa acacaaacaa actctgggaa
     1921 aatagctaac gaaaatactg gagaagtatc caataaacct agtgattcaa aaccaccagt
     1981 tgaagaatca aatcaaccag aaaaaaacgg aactgcaaca aaaccagaaa attcaggtaa
     2041 tacaacatca gagaatggac aaacagaacc agaaccatca aacggaaatt caactgagga
     2101 tgtttcaacc gaatcaaaca catccaattc aaatggaaac gaagaaatta aacaagaaaa
     2161 tgaactagac cctgataaaa aggtagaaga accagagaaa aaactcgaat taagaaatgt
     2221 ttctgatatt gagttgtata gtcagacgaa tggaacctac agacaacatg tttcattgga
     2281 tggaattcca gagaatacgg atacttactt tgtcaaggta aaatcttcag catttaaaga
     2341 tgtctatata ccagtagctt caataactga agagaaaaga aatgggcagt cagtttataa
     2401 aatcacagcc aaagctgaga aactccagca agaactagaa aataaatatg tcgacaattt
     2461 caccttctac ctcgataaga aggctaaaga ggaaaataca aactttactt cctttagcaa
     2521 cctggtcaaa gctataaacc aaaatccctc tggaacctat catttagcgg ccagcctgaa
     2581 tgctaacgaa gtggagcttg gtcctgatga aagatcctat atcaaggaca cctttactgg
     2641 tcgtttaatc ggtgaaaaag atggcaagaa ttatgctatc tataatttga aaaaacctct
     2701 gtttgaaaac ttgagtggtg ctacagtaga aaaactgagt ctaaaaaatg ttgctatttc
     2761 agggaaagat gatatcggtt cactggcaaa tgaagctcag aataatacaa aaattaagca
     2821 agttcacgtc gatggtgttc tggctggtga acgtggtatc ggtggtttgc tggctaaggc
     2881 tgagcaatca agcatcacag agagcagttt caagggaaga attatcaaca cttatgaaac
     2941 gactgctgcc tacaatatcg gtggtatggt cggtcatttg acaggtgaca aggctttact
     3001 tactaagtca aaagcgacag tagccatttc atctaacaca aatacttcag atcagactgt
     3061 gggtggactt gcaggcctag tagaccgaga tgcacagatc caagatagct atgctgaagg
     3121 tgatatcaac aatgtcaagc actttggtag agtcgctgga gtggcaggca atttgtggga
     3181 tcgaacttct ggtgatgtta ggtatgctgg aagtttgacc aatgttctca gcgatgttaa
     3241 tgtaaccaac ggaaatgcca tcactggtta ccactataac gaaatgaagg taaaggacac
     3301 attcagcagc aaggccaaca gagtctacaa tgtcaccttg gtcaaggatg aggtcgtcag
     3361 caaggaatcc tttgaagaaa gaggaacaat gctagatgct tctcaaattg caagcaaaaa
     3421 agcagaaatc aatcctttca ttctaccaac agtggagcca ctttcaacaa gtggcaaaaa
     3481 agacagtgat ttttctaagg tggcctatta tcaagctaag cgcaacttga cttataaaaa
     3541 cattgaaaaa ttgctacctt tctacaacaa ggcaaccatc gtcaaatacg gaaacctggt
     3601 caatgagaac agtcttttat atcaaaaaga actcttgtca gcagtcatga tgaaggacaa
     3661 ccaagtcatc acagacattg tttctaacaa acagactgca aacaaactct tgcttcacta
     3721 caaggatgat ttatctgaga agctggatct caaataccag aatgatttcg ccaaattagc
     3781 agaatatagt ctgggcaata ctggacttct ctatacgcca aaccaattcc tgtatgacca
     3841 aacctctatc atcaagcaag tcttacctga cttacaaaag gttgactatc attcagaagc
     3901 catcagaaag acgctgggta tttctccaaa cgtcaagcaa actgagctct atctagaaga
     3961 ccagttcgcc aaaacaaaac aacaactgga agacagtttg aaaaaactct tgtcagcgga
     4021 tgctggactg gctagtgcta acccagtcac tgaaggttat cttgtagata aaatcaaacg
     4081 caacaaggaa gccttgctac ttggcttgac ctatctggaa cggtggtata actttagcta
     4141 tggtcaggtg aatgtcaaag acctagttct gtaccatttg gacttctttg gtaaggggaa
     4201 tgcttcacca ttagatactc tgattgagtt gggtaaatct ggctttaaca atcttctagc
     4261 taagaataat gtcgatactt atggtatcag tcttgccagt caacatggaa cgacagattt
     4321 gtttagcacg ctggaacatt accgaaaagt ctttttacca aatacaagca ataatgactg
     4381 gtttaaatca gagactaagg cttacattgt cgaagaaaaa tccactattg cagaggtcaa
     4441 agccaagcaa aagcaagcag gaactaagta ctctatcggt gtttatgacc gaattactag
     4501 taatacttgg aaataccgca atatggtctt gcctctcctg accttgccag agagatccgt
     4561 atttgtcatc tcgaccatgt ctagtctagg atttggagct tatgatcgct accgcagtag
     4621 tgaccataaa gcgggcaagg ctctcaatga ttttgttgaa gaaaatgcgc gtgaaacagc
     4681 caaacgtcag cgagatcact acgattattg gtatcgtatt ttagatgaac agtcacgcga
     4741 aaaactctat cgtacgattc tcctttatga tgcctataag tttggggatg atacaacatc
     4801 aggaaaagct acagcggagg ctaagtttga tagctccaat ccagctatga agaacttctt
     4861 tggaccagtt ggcaataagg tagtacacaa ccagcatgga gcctacgcta caggggacgg
     4921 cgtctactat atgtcttacc gcatgctgga caaggatgga gccattactt atacccatga
     4981 aatgacccat gattcggatc aggatattta ccttggtggc tatggtcgaa gaagtggctt
     5041 gggaccagag ttcttcgcaa aaggcttatt gcaagcccct gaccaaccaa gtgacgcaac
     5101 cattaccatc aattctatct tgaaacactc aaaatcagat agtacagagg gatcccgtct
     5161 tcaagtcttg gatccgacag agagattcca aaacgcagca gatcttcaga actatgtcca
     5221 taacatgttt gaccttatct acatgatgga atatctcgaa gggcagtcaa tcgttaataa
     5281 actaagtgtt taccagaaaa tagaagctct cagaaaaatc gagaaccaat atctaacaga
     5341 tcctgctgat ggaaatgatg tttatgctac taatgttgtg aaaaatttga ctgaagatga
     5401 agctaaaaaa ttgacgagct ttgatagttt gattgacaat aacattcttt cggctcgtga
     5461 gtataaggct ggcacttatg agagaaatgg ctactttact ataaaactct ttgcaccaat
     5521 cttttcagca ttgagcggtg agaaaggaac tccaggtgac cttatgggac gaaggatagc
     5581 ttttgaactt ctagctgcta aaggatttaa agatggtatg gtaccatata tttccaacca
     5641 atacgaagaa gatgctaaac aacaagggca aactatcaat ctttatggta aagaacgggg
     5701 cttggtgacc gatgagcttg ttttgaaaaa ggtatttgat ggtaagtata aaacttgggc
     5761 agaatttaag acagctatgt accaagaacg ggtggatcag tttggaaact tgaagcaggt
     5821 gacctttaaa gatccgacaa aaccatggcc aagatatggc acaaagacta tcaataatgt
     5881 ggatgaattg cagaaactaa tggatgaagc tgttttgcag gatgctacag gtacacgttg
     5941 gagtaattat aatccagaaa cggacagtgc cgtccataag ttgaagagag caatctttaa
     6001 agcctatctt gaccaaacaa atgattttag aagttcaatt tttgagaata aaaaatagtg
     6061 tttactatta ggaaataaag tttaaaaagg tgatgaagaa caaaccaaga ttcaagcagg
     6121 aattcctcct gataatgaag taagttatgc tcttatttat cagcaggaaa ctcttcctgc
     6181 aacaggttca tcaacttctg cgcttacagc tttaggccta ttagctgttg gtagtttagt
     6241 tcttttggtt cataatatga cgggaacagt tttttgctcc ctctgaaaag tcatcatttg
     6301 atggcttttt tctatatagg gtaaaagata gggtaaaagg ctatcatcgg acaaaataaa
     6361 gaaggcatga tataatataa agtagatttc tatgtcataa aacaagaact atttggacat
     6421 cattcatttg aaaactctct atgttcaaac tatagtaaaa taaaataggg gatctaaatc
     6481 cttgctacga aaggaaaaaa actcaatggc tactattcaa tggtttcctg gtcacatgtc
     6541 taaagctcgt cgacaagtgc aggagaattt aaaatttgtt gattttgtga cgattttagt
     6601 tgatgcacgc ttacctctat ctagtcaaaa tcctatgttg atcaagattg ttggtgataa
     6661 accaaaactc ttgattttaa acaaggccga cttggctgat ccagcaatga ccaaggaatg
     6721 gcgtcagtat tttgaatcac aaggaatcca gacgctagct atcaactcca aagagcaagt
     6781 gactgtaaaa gttgtaacag atgcggccaa gaagctcatg gctgataaga ttgctcgcca
     6841 gaaagaacgt gggattcaga ttgaaacctt gcgtaccatg attatcggga ttccaaacgc
     6901 tggtaaatca actctgatga accgtttggc tggtaaaaag attgctgttg ttggaaacaa
     6961 gccaggggtc acaaaaggtc aacaatggct taaaaccaat aaagatctgg aaatcttgga
     7021 tacaccgggg attctctggc ctaagtttga ggatgaaact gttgcactta agttggcatt
     7081 gactggagct atcaaagacc agttgcttcc tatggatgag gttaccattt ttggtatcaa
     7141 ttatttcaaa gaacattatc cagaaaagct ggctgaacgc ttcaaacaaa tgaaaattga
     7201 agaagaagcg cctgtgatta ttatggatat gacccgcgcc ctcggtttcc gtgatgacta
     7261 tgaccgtttt tacagtctct tcgtgaagga agtccgtgat ggcaaactcg gtaactatac
     7321 cttagataca ttggaagacc tcgatagata cattggaaga cctcgatggc aacgattaaa
     7381 gaaatcaaag aactccttgt gacagtcaag gagttagaaa gccctatttt tttagatctt
     7441 gaaaaggata atcgctcagg agttcaaaag gaaatcagca agcgtaaaag agccattcaa
     7501 gctgaattag atgaaaattt gcgcttggaa tccatgcttt cttatgaaaa agaactttat
     7561 aagcaaggat tgaccttaat tgcaggtatt gatgaggttg gtcgtggtcc tcttgctggt
     7621 cctgtagtcg ctgcggccgt tattttacct aaaaattgta agattaaagg tctcaacgac
     7681 agcaagaaaa ttcctaaaaa gaaacatctg gagattttcc aagccgttca agaccaagcc
     7741 ttgtcgattg gaattggtat catagataat caggtcatcg accaagtcaa tatctatgaa
     7801 gcaaccaaac tagccatgca agaagcaatc tcccagctca gccctcaacc agagcacctt
     7861 ttgattgatg ccatgaaact ggacttgccc atttcacaaa cctccattat caaaggagat
     7921 gccaactccc tctctatcgc agcagcatct atagtagcca aggtaacacg tgatgaattg
     7981 atgaaggaat acgatcagca gttccctggc tatgatttcg ctactaatgc aggatatggc
     8041 acagctaaac atctggaagg cctcacaaaa ctaggagtta ccccaattca ccgaaccagc
     8101 tttgaacccg ttaaatcact ggttttaggt aaaaaagaaa gttaattgaa aggaaatacc
     8161 atggaggaac agtcggaaat agtccgttct aagaaagaat tcgcctttgc atccagcact
     8221 atactatccc aagttggtcg aggaatcatt gtcggcctca tcgttggaat tatcgtcgga
     8281 tcctttcgtt tcttaattga aaagggcttc cacctgatac aaggagttta tcaagatcaa
     8341 gggtacttag tacgcaatct ttttgtactg gttttgtttt atatactcat ctgttggctc
     8401 agtgccaaac taacacggtc agaaaaagat attaaaggct caggaattcc tcaagtcgaa
     8461 gccgaactga aaggcctcat gtccctcaac tggtggggca ttctttggaa aaaatatgtg
     8521 ctaggtattc ttgctattgc cagtggactc atgctgggtc gagagggacc cagtattcaa
     8581 cttggagcag ttggtggtaa aggaattgcc aagtggctca aatccagtcc agtagaggaa
     8641 cgttccttga ttgccagtgg agctgcagca ggtttagccg cagcctttaa tgctcctatt
     8701 gcagcacttc tctttgttgt agaagaagtc tatcaccatt tttcgcgctt tttctgggtc
     8761 tcaactctag cagccagcat cgtagcaaac tttgtgtctc tactcatgtt cggtttgaca
     8821 ccagtattgg atatgccaga taacattcct cccatgaccc tagatcagta ttggatatat
     8881 ctcgtcatgg gaattttcct tggattttca ggctttctct atgagaaagc tgtattaaac
     8941 gttggaagag tttatgactt gattggtcaa aaaatccatt tggatagagc ttattatccc
     9001 atcttggctt ttatccttat cataccagtc ggaatcttct tacctcaaat cattggtggc
     9061 ggaaatcagc ttgtcctttc tttaactgaa caaaatttta gtttccaagt tttattagct
     9121 tactttttga tccgctttat ttggagtatg attagctatg gaagtggact gccaggagga
     9181 attttcctcc ccattttagc tcttggttct ttgcttggtg ccttaattgg tgctatctgt
     9241 gtcaatcttg gacttgtcag tcaagagcaa ttccctatat ttgtcattct aggaatgagt
     9301 ggctattttg gagccatatc aaaagctccc ttaaccgcta tgatcctcgt aactgagatg
     9361 gtaggagata ttcgcaacct catgccactt ggtcttgtca ctcttgtttc ttatattatc
     9421 atggatttgc tcaaaggtac gccagtctat gaagccatgc tggaaaaaat gcttccagaa
     9481 gaagtatcta gcgaaggaga agttacactt atcgaaatac cagtttctga taaaattgct
     9541 gggaaacaag ttcatgaact caacttacca cacaacgtcc tcatcacaac tcaagtccat
     9601 aatggcaaga gccaaacagt taacggctca accagaatgt atctgggtga tatgattcac
     9661 ctggttattc caaaaagtga aattggaaaa gtcaaagatt tgttgttgta gtacaatcat
     9721 ttac
//
LOCUS       DAVHIU010000065         9644 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVHIU010000065 DAVHIU010000000
VERSION     DAVHIU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.067"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1193)
                     /locus_tag="VVN06_001377"
     CDS             complement(<1..1193)
                     /locus_tag="VVN06_001377"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5562902.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRIDDLIQFSKKHNIPLITIKELQEYRKAYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKIIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILN"
     gene            complement(1213..1848)
                     /gene="ribE"
                     /locus_tag="VVN06_001378"
     CDS             complement(1213..1848)
                     /gene="ribE"
                     /locus_tag="VVN06_001378"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5562903.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(1833..2933)
                     /gene="ribD"
                     /locus_tag="VVN06_001379"
     CDS             complement(1833..2933)
                     /gene="ribD"
                     /locus_tag="VVN06_001379"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5562904.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTIDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(3067..3181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3338..3931
                     /gene="ruvA"
                     /locus_tag="VVN06_001380"
     CDS             3338..3931
                     /gene="ruvA"
                     /locus_tag="VVN06_001380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5562905.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            3941..4504
                     /locus_tag="VVN06_001381"
     CDS             3941..4504
                     /locus_tag="VVN06_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5562906.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            4501..5178
                     /locus_tag="VVN06_001382"
     CDS             4501..5178
                     /locus_tag="VVN06_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562907.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(5315..6346)
                     /locus_tag="VVN06_001383"
     CDS             complement(5315..6346)
                     /locus_tag="VVN06_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU5562908.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(6601..7284)
                     /locus_tag="VVN06_001384"
     CDS             complement(6601..7284)
                     /locus_tag="VVN06_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU5562909.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMNSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(7296..8240)
                     /locus_tag="VVN06_001385"
     CDS             complement(7296..8240)
                     /locus_tag="VVN06_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5562910.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(8401..9566)
                     /locus_tag="VVN06_001386"
                     /pseudo
     CDS             complement(8401..9566)
                     /locus_tag="VVN06_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2746 a         1850 c         1884 g         3164 t
ORIGIN      
        1 atatttaaaa tgtgacccat tcgatttttc tttgtttcta aataaaaact atcgtaagga
       61 ttggcttcta tttcgattga tattctactg gaaatggtaa ttccatattt ttctaactgt
      121 tcaaccttgt caggattatt tgtcagtaaa tgaagtgact gaagtcccag atctttaagc
      181 atttgtgctc caatatgata ttctcttaaa tcaccttcaa agcctaatgc aagattggca
      241 tcaagcgtat ccatgccttg atcttgtaaa tgataggctt ttaatttatt gataagtcca
      301 attcctcgtc cctcctgtcg caagtaaagt aagacacccg aaccattctc aacaattatt
      361 ttcatagctt tatcgaattg ctgtccacaa tcgcaacgta aagagcctaa aacatctcct
      421 gttaaacatt cggagtggac ccgacataat acattggctt catcctctat gtttcccata
      481 ataagagcaa gatgatgttc cccatttagt ttatctatat agctaattgc tttgaaatta
      541 ccgtatctag taggcatatt gacagttgaa actcgttcta ccagctgatc atatgctttt
      601 ctatattctt gtaattcttt gatggtaatt agtggaatgt tgtgtttctt cgagaactga
      661 attaaatcat ctattctcat cattttgcca tcatgattca ttatttcaca acataggcca
      721 cactctttta gtccagctaa ttttaataaa tcaacagttg cttctgtgtg tccatttctt
      781 tctagaacac cacctttttt tgcaattaaa ggaaacatgt gtcctggcct gcgaaaatca
      841 gagggtgtta tatcttcagc tacacacata cgtgcggtca gtcctctctc ctcggcagaa
      901 atacctgtgg tcgtttcttt ataatcaatt gaaactgtaa aagcagtctt atgattatct
      961 gtattgtttt caaccatagg tgaaagcatt aattgattag ctaaactttc gctcataggc
     1021 atacaaatta atcctttggc ataagtagcc ataaaattaa cattttctgt tgtagctgct
     1081 tgtgcagaac aaattaagtc tccttcattt tctctatcct tgtcgtctat aacaagaaca
     1141 agtcgtccct tctgcaatgc ttctaatgct tcttgtattt ttcgatattc cattgactga
     1201 ttatcctttc tgctaaaatc cattttgata taatagttcc ttcgatattt ctgattttgg
     1261 agagttatcc atcagttttt gcacatattt acctaagata tcattttcaa gatttactgt
     1321 actcccgact tgtttactct taagaatggt ttgtttcaag gtatgaggga taacagatac
     1381 tgaaaagttt actttggaga ctttagcgac agtcagacta atgccgtcaa ttgtaataga
     1441 tcctttttca actattaaat ctaaaatttc tttttgtgtg ttgatttgat accatacagc
     1501 attatcatct ttttttattg acgagatttt tcctgtacca tcaatgtgtc ctgtaacgac
     1561 gtgaccccca agtcgaccgt tgacagataa ggctctttct agattcacct cacttccatg
     1621 ttttaataga gtaagagctg ttcgactcca tgtttcattc attacatcaa ctgtaaagga
     1681 ttgatgattg aaatgagtaa ctgtaagaca gataccattt actgctatac tatcgcctaa
     1741 atggatatcc gttaatattt ttgaggcttt aattgatagt ttacaattac gagagtcttt
     1801 ctgtattctt tcaacttttc cgatttcttc aattattcct gtgaacatgg ataaatcact
     1861 tcactttcta tgagatagtc atttcctatt tgagaaaaag cataaggttt caatctaata
     1921 gcgtcatttg gcaaagaaat gccttcacct ccgacaggaa acttggcact gcctccaaaa
     1981 atttttggtg caatatatat tttcagctca tcaacaattt gttgttccaa agcactccaa
     2041 ttcattagac tgcccccttc tagaactagg ctatcaatct gcatgtttcc tagatgttgc
     2101 attaaactcg ataagtctat atgattgcct tttttcttta tggaaagtat ttcacagcca
     2161 tgattttgat atagcttcat tttatttttg tcttcagagg aagtggcaat gtaagtttta
     2221 atatcatttg ctgtttttac gattttagag gtaagaggag ttcgtaaatg tgtatcgcat
     2281 atgatacgga taggattttt cccttcctcc aatctacatg tcagcaaagg atcgtcttga
     2341 ataacagtat tgactcccac cataattgca ctaacatggt gtcgtaactg atgcacatgc
     2401 tttcttgcct cttcttcagt aatccatttg gattgatttg ttttagtggc tatttttcca
     2461 tcaattgtca ttgcatattt cataaaaaca tagggtacat gctgggtaat atactttcta
     2521 aaacttttta ttaagttaag acactcattt tctaaaattc caacagtaac ttgaagatta
     2581 ttttcctcaa gtatctttac tccttttcca gatacaatag gattacagtc taggcttcca
     2641 atgactactc ttgtaatacc actatcgatt atagcatcta tacaaggagg tgttttcccg
     2701 aagtgacaac agggttcaag tgttacataa agcgtcgctc cgacagggga ttctctacag
     2761 tttttaagag catttctctc agcatgtgag ccaccaaaaa actcatgata accttgtccg
     2821 atgatgtgat tatcttttac aataactgcg ccgaccatag gattgggatt gacgtaacca
     2881 gccccttttt gtgccagttt tattgctaat ttcatatatt ttgaatcgct catctcacta
     2941 cctccaaaaa aatatacctt gaataggaga ctactcaagg catacaaaag aaaacttatg
     3001 cgattaacaa aaatgctctg aaatgacaag taatcatttc agagcacgca aaaagcacaa
     3061 atatactttt atcttctttc atccagacta tactgtcggc tttggaattt caccaaatca
     3121 tgcctttcgg ctcgtgggct ataccaccgg tagggaattg caccctgccc tgaagatagt
     3181 tattcaatta cagatgatta tagtacttaa ttttgaatat gtcaacagat aaataccgat
     3241 tgtttttgat atactgtatt tgtgataatc gattctcgct cctcggataa agaaaatatg
     3301 atatactaga taaacgaaat aagagagaag gaacactatg tacgcatatt taaaaggaat
     3361 cattaccaaa attactgcca aatacattgt tcttgaaacc aatggtattg gttatatcct
     3421 gcatgtggcc aatccttatg cctattcagg tcaggttaat caggaggatc agatttatgt
     3481 gcatcaggtt gtgcgtgagg acgcccattt gctttatgga tttcgctcag aggatgagaa
     3541 aaagctcttt cttagtctga tttcggtctc tgggattggt cctgtatcag ctcttgctat
     3601 tatcgctgct gatgacaatg ctggcttggt tcaagccatt gaaaccaaga acatcaccta
     3661 cttgaccaag ttccctaaaa ttggcaagaa aacagcccag cagatggtgc tggacttgga
     3721 aggcaaggta gtagttgcag gagatgacct tcctgccaag atcgcagtgc aagcaagtgc
     3781 tgaaaaccaa gaattggaag aagctatgga agccatgttg gctctgggct acaaggcaac
     3841 agagctcaag aaaatcaaga aattctttga aggaacgaca gatacagctg agaactatat
     3901 caagtcggcc cttaaaatgt tggtcaaata ggagcagaga atgacaaaac gttgttcgtg
     3961 ggtcaagatg accaacccac tctacatcgc ctatcatgat gaggagtggg gccagcccct
     4021 ccatgatgac caagcgttgt ttgagttgtt gtgtatggaa acctatcagg caggcctgtc
     4081 ttgggaaacg gtactcaaca aacgccaagc tttccgagaa gcctttcata gctatcaaat
     4141 tcactcagtc gcagagatga ctgacactga attggaagcc atgctggaga atccagctat
     4201 cattcgaaat agagccaaga tttttgctac acgcgctaac gcccaagcct ttctacagtt
     4261 acaggcagag tacggctctt ttgatgccta tctttggtct tttgttgagg ggaaaactgt
     4321 cgttaacgat gttcctgatt atcgccaagc cccagctaaa acacccttat ctgagaaatt
     4381 agccaaagat ctcaaaaaac gagacttcaa gttcacaggc ccagtcgccg tattgtcttt
     4441 tctacaggct gcagggctag ttgatgacca cgagaatgat tgtgagtgga aaggtcttaa
     4501 atgatgtcta acaaaaataa gggaattctg atttttgcga ttctctatac agtcctcttt
     4561 gtgtttgatg gcgttaaatt gctggcttct ttaatgccat ctgccattgc aaattatctt
     4621 gtttatgtag ttttagcttt atatggctcc ttcttgttca aggatagatt gatccaacaa
     4681 tggaaggaga ttagaaagac taaaagaaaa ttcttctttg gagtcttaac aggatggctc
     4741 tttctcattc tgatgactgt tgtctttgaa tttgtatcag agatgttgaa gcagtttgtg
     4801 ggactagatg gacaaggtct aaatcagtct aatattcaaa gtacctttca agaacaacca
     4861 ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg tagaagaatt atttttccgt
     4921 caggtcttat tgcattactt gcaggaacgg ttgtcaggtt tactaagcat tattctggta
     4981 ggacttgttt ttgctctgac tcatatgcac agtttggctc tatcagagtg gattggtgca
     5041 gttggttact taggtggagg ccttgccttt tctattattt atgtgaaaga aaaagagaat
     5101 atctactatc ccctacttgt tcacatgtta agcaacagcc tctccttaat cattttagct
     5161 atcagtatag taaaatgaaa tgagaacagg acaaatcgat ttctaacaat gttttagaag
     5221 tagaggtgta ctattctagt ttcaatatac tgtaatatgt gatgaaaatg ccagtaatga
     5281 taccgagaaa aaagctgaga aacttttccc agctttattt gttatagtca aagagaatga
     5341 cttgttcctg tgcatctaca tgagcatgga ccccaaaggg tacaattgct cttggagttg
     5401 cgtggccgac attcagatta tagacaatcg ggatattgct gtcaatgata tccaatagtg
     5461 cctctttata gtcgtcatgg aaagtttcat ccataggttt tccgaccaag agtccattga
     5521 tgaccgcgaa tatgccagtg tcctttaaag ttagcaacat ctttttgaag tcttctggct
     5581 taggcttttc ttcgcttgtt tcgagcaaga ggattttccc ttcccagtct gacaagtcag
     5641 ggaaaagttt gtatttttgg cagagttccg tgctatctgc gtatcgagag ttgtcaaaga
     5701 tatcgtagag ggattcgagg caaccaccga ggattttccc ctcgaactga gcacttcctt
     5761 gcaacaagtc aaaacctgta tttgtatgac tgacacgagg tgttcccagg gccgtgggac
     5821 taaaatcagt tcgttcctca taccaaacgt cactagggcg gatttctgaa attcttcccg
     5881 tctcaatcaa ttctttaaag tagtgaaggc tataggctag catttctttg tctaattcac
     5941 aaatgtctgc taaaaaggat tgaccataaa aagtcttgat tcctaattta tgcaacatga
     6001 ggtggttcat ggttgtatcc gagaagccaa gaaaaatttt ttgtttgata accttttgga
     6061 gttggtcatt ttcaaaaaga taaggtagca agcgataggt atcgtctcca ccgatggcac
     6121 ataggatcat gtcgatgcta tcatcagaaa aggcatgaat caaatcctct gcacgagctt
     6181 caggatggtc cttgataaag tctaatcctt ttaacgaatg gggcaaaaag atgggattga
     6241 gtcccagatc cttgagacgt tggataccca agtccacttc gtgtttgaca aaatcctctc
     6301 cgataatgcc actagataaa ctaacaatac caatagtaga aaccatatct catcctccta
     6361 gaaatagatt gagcctattt tatcacaaaa tataagagaa agctatgtgg gatttcagaa
     6421 gaatactttt aatactcttc gaaaatctct tcaaaccacg tcagttttat ctgcaacttc
     6481 aaagctgtgc tttgagcaac ctgcagctag cttcctagtt tgctctttga ttttcattga
     6541 gtacaactca aaaaagtaag tgaaaaagca accgcacctg cagttgcttt tatcgttttc
     6601 ttattcttga tagcgtgttg gtcctgcact tgcactacgg atattcaagc gtttcttgag
     6661 atagaagcga agggctagga gggctgctcc aataatagct agtggcaatg gatccagtac
     6721 tgggttaagg ctagctggta ggaagcttgt tgcaaagaag acaagcaacc aaaggaacat
     6781 agaagctagg ataactagta cagatttcca gaaaggtgga cgttgactgc gatccatatc
     6841 tggtccatag tattggtaaa caaagtagta catcaagtag aaggcaaatc caccaaccag
     6901 tccaactaat agaagagtaa tcaatccata gccgaaagct tgatctgccg caaagaaggt
     6961 tgtgagggcg ctgacaaggg caaagaggct agtgatgaaa agagctgagt tcataatcat
     7021 cagttttgga tcatcatttt cttttggatg ctctttttcg tactgctctt tgacagtgaa
     7081 gctatgagcc caatgagttg gtgcgccgta tagggaacgg gcagttgtac ctttagattg
     7141 ctcctcaagg atttggggaa tgacttcctc aaaaatagtc tggatttcag cgtctgtttt
     7201 cccatctttg atgaattgtt gggtagcaat gtggacaaac tcttggtttt tcttagttaa
     7261 tttttgtaga tcaatttgag acataggaac tcctcttaga accatttttt atggatgaga
     7321 tagagagtga gcgagacact catagcaaag gcgataaaga cgattaacca gaaggcattt
     7381 ggctctccgt ttaggggaat ttcattatcc ttaaagttca tcccgtaggc agaaaagacc
     7441 atggttggga tggacatgac gatggtcaca agggccaagg ttttcatgat gttgttctgg
     7501 ttgttagaaa tgatagaggc aaaggtctct gtcatagaat gcaagacgtt tccataaata
     7561 tctgccatct cgatggcctg ttgggtttca atcagggtgt cttcaagcag gtcttcgtcc
     7621 tcaaggtatt tcttgatatt gctggttgaa ctggtcaatt tcttaatcac gcgctcattt
     7681 gttttgaggg aggccttgaa atagacgata gttttttcca attccatgag ctcaatcaat
     7741 tcttcattac gagttgattg atgcagttga ctttcgattt gttcactctt gcggtcgatt
     7801 gaacgaaggg ctgttaggta aagctctgca ttgcgataaa gaatttgaaa gataaaacgt
     7861 gaacgcatga aggtatagaa attacgcaat cgacggttga taaagacatc aaggacaggt
     7921 agtggttcca aacacgtagt gataatggtt tcctcagtga tgataatacc aagcgggatg
     7981 gttacgtagt aggtgcggtt atttctttcc tccgtgaccg gcacgtctac gataatcagg
     8041 gtatactcgt cttcaatggt aatacgagac atttcttccg catcgagcgg tgctcgaagg
     8101 tcggcaatat caatatcgaa ggtgttggcg atttcgagtg attcattttg agtcggattg
     8161 acgagattga tccaagtacc cggttcaagc gtatcgatct ctttaaattc agttgttgta
     8221 gagagaaaaa cttgtttcat atcccttatc ctttctcatt tttcagattt tttcacactg
     8281 tactattata ctacaaaatc ggcttttttc aaataaaatc ttacaaagac accgttgtta
     8341 ttggattttt actttgtctt gaaatgttga tttttaaatt tgctcccctt tttgctcccc
     8401 ctatagtgct tttagtgcag tttcgtagaa tgatactgct ttttttgcgt tctcttttga
     8461 gaggtgacta tatatatcca tggtcattga taaagtggaa tgacctaggc ggtactggag
     8521 ttccttgtat ggtattccag agttaagcaa gagactagcg tgagtgtgac ggaatccgtg
     8581 aaatccaatg ttaggaacat cagcacgctt aaaatgtgtt tgcaatcttc ttttaagtct
     8641 tgaactgcta ggatactcat gaatgaaatc agaaaatacc acgcgttcac gttgccctat
     8701 cttccaggcc tctttagttt ggcgtagttt gtactgcttc agcatgctaa cagtcgcttg
     8761 atctatatct atatcccgat aactagcctt agactttggg ctattgattt ctatatcccg
     8821 atttagtgtt ttggtaatat gcacaacggc attatctaag tcaatatcag accaggaaag
     8881 agctaaagct tcattgatac ggcaaccagt agccaatagg aacttataga gtgttgtctc
     8941 atagtagtaa cgatatctat gagtatctaa attatcgaga taaccaagaa attttttagt
     9001 tcttgatttt caaagtgctt tacttttttc cgctttgctt tctgagtgtt acgaggtaaa
     9061 acaacatcac gcgcaggatt ggacggtata gcctgcatgg ttacgccata ctgtaagata
     9121 cgtttgttta aagcgtgtat cttgtcataa tgtagaaaag ccgctttttc ccctctatta
     9181 gtcttgtcag caagttcgtt gataattgac tggataagtg gagtagtgag cttatcgagc
     9241 ttgtaagccc caaaaatagg caatatatga ttatcgatta gctttttaac attgccttga
     9301 gtgtttggct taaccgtatt cttataactt tccaaccaga gagttgccaa ttcttgatag
     9361 ttttttatgg taacagcctt ttgtctggta gatcctgctt tttgaaaagc tattttttct
     9421 tgattggctt tctgcttaac ttccttttgt gtccgacctg ttactttggt ttttactttc
     9481 tttcctgtta cctggtcaac tcctagatat acattagcac gataaacttt gctaccgtct
     9541 ttttttataa cttcagttat tttcatgatg ataaaccttt ctaaaataca tcagcaggca
     9601 agccattatc aaaaaggttt tatatcatgc taggagctac gaga
//
LOCUS       DAVHIU010000066         9563 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVHIU010000066 DAVHIU010000000
VERSION     DAVHIU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.068"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..337)
                     /locus_tag="VVN06_001387"
     CDS             complement(<1..337)
                     /locus_tag="VVN06_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562911.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLV"
     gene            complement(1016..1648)
                     /locus_tag="VVN06_001388"
     CDS             complement(1016..1648)
                     /locus_tag="VVN06_001388"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5562912.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(1682..2383)
                     /gene="pyrF"
                     /locus_tag="VVN06_001389"
     CDS             complement(1682..2383)
                     /gene="pyrF"
                     /locus_tag="VVN06_001389"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5562913.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(2616..3836)
                     /gene="divIB"
                     /locus_tag="VVN06_001390"
     CDS             complement(2616..3836)
                     /gene="divIB"
                     /locus_tag="VVN06_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU5562914.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEKSEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKAFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(3846..4904)
                     /locus_tag="VVN06_001391"
     CDS             complement(3846..4904)
                     /locus_tag="VVN06_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5562915.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(4906..6258)
                     /locus_tag="VVN06_001392"
     CDS             complement(4906..6258)
                     /locus_tag="VVN06_001392"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5562916.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMAAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(6362..7003)
                     /locus_tag="VVN06_001393"
     CDS             complement(6362..7003)
                     /locus_tag="VVN06_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5562917.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7005..9026)
                     /locus_tag="VVN06_001394"
     CDS             complement(7005..9026)
                     /locus_tag="VVN06_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5562918.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFIYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDIENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNILYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(9097..9396)
                     /locus_tag="VVN06_001395"
     CDS             complement(9097..9396)
                     /locus_tag="VVN06_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU5562919.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWNGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
BASE COUNT         2821 a         2122 c         1701 g         2919 t
ORIGIN      
        1 agactagaaa agaagaaata attgcagtac tacctacaat agctagatac ctctttttag
       61 aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt atctgactag
      121 aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat cctcgaatag
      181 aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg aaagcaagaa
      241 aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaac cgaggtgatt
      301 gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct tataataaaa
      361 atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt aaggcttact
      421 ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa ttcttacatg
      481 atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat tgtcaccacc
      541 tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg gggtaatatc
      601 tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac cagtgctgtc
      661 actattatta attccagcag aggcgttttc tttctcaagc tcactagttg tatccgaacc
      721 ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg tagggagaaa
      781 caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct tttcatgaca
      841 tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata catattacat
      901 tttcattata tcaacaaaag aaagcgttgt caattatttt atttaaaata ttgtcatatt
      961 ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta ctgacctaac
     1021 cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg ccctctggcg
     1081 tgatgtaacc ttcttcttgg gctagatgga taagctcgct atagtttgaa agcgtcacaa
     1141 gtttaacacc agcatctgca aagttcttat ctgcttttgg caattggtag ctgaaaatcg
     1201 ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct tcaagaactg
     1261 aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga gctacgcgac
     1321 cttcgatttg attaccagct ccgtggtctt ttggtttgct acgaatgtag gcaaaaggca
     1381 agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca gttcctgcaa
     1441 tcacttctac ttcaggaaag gcttctttga tagcttccac aaaaccattt tcaattaggg
     1501 tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt gacttgatac
     1561 cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt ttcaagaggt
     1621 ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct aatctagttc
     1681 tttaattcca gtcctgtgtc cattcatcct tgatggcatg ataagctgca acaggatcct
     1741 cagcttgggt aatgggacgt cccactacga tatagtcact gccgatttga taggcatcag
     1801 caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga atccctggtg
     1861 tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc tgagccgagc
     1921 aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc acagactctt
     1981 gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt gatgtgagct
     2041 gcgttacagc gatcaatttg gcttgactcc caagaccttc acgcgccgcc ttcatcatct
     2101 ctacaccacc agccgcatgg acattggtca tatccacacc aagctgagac aagaccttca
     2161 tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag acactatgac
     2221 ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc tccatcccta
     2281 ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg accgcctcaa
     2341 aactaggaaa atcaagagca atgattggac gatgttctcg cataggtttc tccttttacc
     2401 tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg ccaacagcaa
     2461 ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc tgcctctctg
     2521 gactgcttta aaggtttttt actattctat tatttctact agcacttgtc aagtaaaaac
     2581 atggtcacta aagaactata agagaaaagg taaacctagc gacgcgatga gcgctgggtc
     2641 gtttgatttc gattgctctc ttcctcttgt tttttctgtt cttcttctaa acgtttctgt
     2701 tcttcttcct gttttttctc ggcttcctta gcctcttgtt tggctttttc ctcagcttcc
     2761 ataattaatt tatccgccac agtgtaactg taaattccag cttccatgtc aatcacactc
     2821 ggttctgaca attgtggttt aatcttacta taataaggca gtttcttact catttcagat
     2881 agaggaacca agacttcgtc cgaatcattc atggtcaatc gaattaaatc ggatgtcacc
     2941 ttgcttgggg ctaattccac cttttggata gccgccttga gttctgggct aatttgagca
     3001 agttctgaga caaaagcctt gatttgttca ctatcattaa agagaactga taaataagtt
     3061 tctggtaaac tgttcaggct cacagaacta gtctcaagct gaccactgga aagaatagga
     3121 taatgatttt caccagaaat atagtaggcc acaatatcat attccttgac cttaatagtg
     3181 aacttagttg gaaattgata gacaagttga gctgattcaa cccaatagtt agacttaatc
     3241 tgcttttcat attttgcctt gtctagcaga aggttaatcg tataatccga atcctgaatg
     3301 cctgaagcct gtcgaatatc atcagctgta gtttgcaccg ttccctcaac acgaatatct
     3361 ttcatggtcg cataaggact gagcaagtag gcagagacaa tcaataaaag cagacttgga
     3421 aataaaatcg tgaaggctcg caagatatgg ataccaggaa tctttgcttt ggctggtttt
     3481 tcctttatag cctttttagc aagcttttta tcctgttcct ccttctcttt agactctgat
     3541 tcttctttct cttctttctc tttgtcagcc tctgaggatg ctactttttc ttcagacttt
     3601 tccttagctg attctgaatc ttcctggtct gtttcactct cctggtcctg tttgtcctct
     3661 gacttctcag attcttctcc catccgagct tgtctttcct tttccttctc ctcagctaga
     3721 gccgcttctt cttcagcctt cttttttaga tattcttggt ttcgtttctg ccattctgat
     3781 aactctttca attcttcgag gatttctttg tcctcatttt tcttatcttt tgacatttac
     3841 tttccttatg ataaatcttt tttcaacaat tgataaaaat ctgctagaga tttcaattcc
     3901 ttagaagctt tcatcttagc ttggtaatct tccttgtgac ttagtaagtg agaaagcttc
     3961 tcttccaaac tatccaaggt caaatcgctt tcttgaaggt cttctgcata gcctttctta
     4021 acaaagtaag ctgcattttc aatctggtca ccacgactag cttcacgacc aagtggcaca
     4081 atgacatgca attttgctat cgccaagagc tcaaaaatcg tattggcacc accacgtgtc
     4141 acaacaatat cagccaattc catcaagggt tgatagagat cggtcacata gtcaacacga
     4201 aaaagatttt ggctcaactc attcaggcta gaatctccag ttagattgat aatattgtag
     4261 cgctctgtta gttctttctt atggtctgtc accaattggt taaagacacg agcacctgca
     4321 gaaccgccaa caaacaatac agtcggcaat ttatgattaa agtggctttg aatatccacc
     4381 aattcatctg gttctggatt tttttgatct gaaaccttgg tcactgctcc cacatgctca
     4441 accttagaca aactcgaagc ttgctcaaag gttgaataca tcttagtcgc aaacttatag
     4501 gcgattttat tggccaagcc catagacagg tcagattcgt gaataaagac aggcactcct
     4561 gacacacgcg cagcgataac aggcggtact gagacaaagc ccccctttga aaaaagggtc
     4621 tgtggacgaa gtcgcaacat gataaagagc gattggacga ttccccagcc aactttgaag
     4681 acgtccagca tattttgcca agagaaatag cgacgcaatt ttccagtcgc aatagaatgg
     4741 aaggtgacat ccaaacctga cttaaggatt tcttggtgtt caataccacg cttgtccccg
     4801 atatagtgga cttcccaacc atcttcgatg aacttgggca ttaacaaaag attgagggta
     4861 acgtgtccaa ccgtcccccc acctgtaaag acaatttttt tcatattatt cttttaactc
     4921 cgctactgtg tcgataaaga ggtcgccacg tacttcaaag ttagcataca tatcccagct
     4981 ggcattggca ggactaagaa gaaccacatc tccttgagtc gcaagctcat aggccttgcg
     5041 ggtcgcatct gcaatatctg tcgcctccac ataagcgaca ccagccttgt ctgctgcccg
     5101 tttgacacgt tctgcagatt gacccaggat gaccatcttc ttgagtccag taatgtctgg
     5161 caccaattcg tcaaactcat tgccacggtc caaaccacct gcaatcaaga cgaccttgct
     5221 gttgtcaaat cctgacaagg ctttttgagt agccaagata ttagttgatt tactgtcgtt
     5281 atagaattta acacccttga tgtcatccac aaactggaga cgatgtttga caccaccgaa
     5341 ggctgaaaga gtttccttga tggtttgatt gtccacacca cgaagcttgg ctacagcaat
     5401 agtcgcaagg gcattttcca cattgtggct acctggaaca ccgatttcat tcgctgccat
     5461 gactacttca ccacggaagt agagttgacc atcttccaga taagctccat caaccttttc
     5521 aagtgttgaa aatggtacaa cagtggcttc tgtcttggaa gccaagtctt ttgccaagtc
     5581 ttgattaaag ttcaagacaa ggaaatcagc tgctgccatc ttgttctgga tattccactt
     5641 ggctgctaca tattcctcaa ataatccatg ataatcgata tgagttggca taaggttagt
     5701 aataaccgca atctcaggat ggaattcttg aacacccatg agttggaaag aagaaagttc
     5761 cataacaagc gtgttcttgt ctgtcgctat ttgagcaacc tgactagcag gatagccgat
     5821 atttcctgat aaaagaccat gttgcccagc agcagtcaaa acttccccaa tcatagtcgt
     5881 tgtggttgtc ttaccgttcg atcctgtgat accaataatc ggtgcttctg aaatcaaata
     5941 agccaattcc acctcagtca agactggaat tcccttggcc aaagcctttt caatcatggg
     6001 attgttgtag gggatacctg gatttttcac cataagggca aactcttcat ccaagagttc
     6061 caaaggatgg ccacctgtaa tgaccttgat cccttcttcc agcaaacatt gggcagctgg
     6121 attgtcctcg aatggtttcc catcatttac tgtcacaatg gcacctaact tgtccaacaa
     6181 acgagccgca gattcaccag acttggccaa acctaaaaca agaactttct tatttttaaa
     6241 ttgatctatt actttcatgt ctcgaactcc atttctactc ctactatttt accattttta
     6301 tggaaataaa aaagccacaa agtgtgtttg tgactctttc ttctaactga atcttaccat
     6361 atcatctatg tgataaatcg gtaactcgaa tgacctgatc cacttgctcc caaatcagag
     6421 gattgtgggt cgcaataata atggtccgat tcggattttt taaagattct aggatggaaa
     6481 gtaattcctc agagtttttg gggtctaagg aagcggttgg ttcatctgca agaatcaaag
     6541 gcggatcctt taaaattatc ttcgctagtg caacacgttg tgcttctcct cctgataact
     6601 caaatatagg ttgcttcaaa tccaaataag agaggtttac acggtttaga gcttgtttca
     6661 tcaaagagat tttctctttt tccttcaact ttttaccaac taaacccaga ttgagattct
     6721 ctttgacggt ttggctttca attaagccaa aatcttgaaa taagtatccc aagtaatctc
     6781 taaagaaaac agaaggcttg atgtccttaa gagaagtgcc atcatagatg atttgccctt
     6841 tgtcatatgg ctccaatcgt ccaatcatat tcaagagtgt tgtcttacca cagccacttg
     6901 taccgattaa ggcataaatt ttcccacctt caaaatgaag attcgtatct gaaaatagct
     6961 gacggcttcc aaatttttta gatatattct ttagttcaat catcctattt tcctttcata
     7021 attgtcatag aaacacgaga ttctttctgc gcttgacggt aaagcgtcaa aactgcacta
     7081 gctagaaaga ccaataaagt gagcaagcca atcaccaagt ctcgactgct taaaataaag
     7141 agactagcac caaatacaaa actggcaaat tggctaacca tatactgagc atgtgtttca
     7201 aaaaatcgta aacctgaaat tcgtttaatc aagatatctc ggcggaattg ctcgaaatat
     7261 agaatattga cagaataaaa tagtaacaag gaactagcta tccccacaat agctcctaag
     7321 attaaagttg ctgtttcagt ttgaacttca ttataacgag ttagataaac acttcttcct
     7381 tctttaagat aggatacttg ctcataaatt ccagctttct tcaagagttc tagcccactc
     7441 tcatatcctt tgataaagag ttgttttcca gcattgatag accaactaga taaggatata
     7501 aaactatcac ctgtagaagt cggcgtgaat accactaaaa tcggatcagt caaatactga
     7561 gtggatacgg gattctcacc gttattataa acaaaccgct tttctcccat tgaaagataa
     7621 ctaacgtgcg ctttcatctc ataatccaaa ggagcacttg cctcctcacc agattttcca
     7681 taataactca atctttcttc aaaaactttc ttaagttctg cttctcgaga gcgcaaatgt
     7741 tctgggagca agaggataaa ctcacctttt tggagatggg ctaacttctg tttggtctca
     7801 gcatctacca cgaccttttc cttgtccaaa taactgggac taacatagag cgtattagca
     7861 tctggactat aggtatccag tgtctctccc tgttcatttt ttccttgtgg attggcaaaa
     7921 tggagcagat tatcctttac ataaagagct tgttcttctt cgattgcttc cttggcaaag
     7981 gcataccact tgctctgatt ttctatatct tttcctctat cacctaagcc aaaagaaatt
     8041 tgataatagt ctgctctatc ctgccatgct tgttttgaaa tttcaagttc tttcaatcgt
     8101 tggtaagacg tcaaacctgt cttaacagcg tatcctactg taaagacagc aaccaactga
     8161 cacaataggg ttaaaatcat caggcgttta aggggtaatc tccccttaat aacgggaact
     8221 aatgctttgt aattcaaact cattagataa aggagcatta gtaaaattga aatcgccaat
     8281 aaaaacaaca gatagaaact aatcccaaaa ccataggtgg ctaacaagat aggataaaac
     8341 aaaccttgac taaaaagaac gactccccca cctaggaagg aaaggagggc tgatagaagg
     8401 agccatttga tatcagtaga taaagaatgc cccatgatgg ataagagagt ctgaccagaa
     8461 aagagtttta tacctgctgc tctcatttcc ttaatccgag tgataatcac taaagcaaag
     8521 aaagataagc caaatattgc taaactaatt aaaataaggg gatttagtaa tattcgaaaa
     8581 gcaagaaaat agggcggtat ctttcggtca gcacttgctt tataacccaa atctcctaat
     8641 ttatcggcaa gcttttcttt cgtcaaggaa cctgacaaaa ggagataact atttagcgga
     8701 tcactacgtt cacgactttc ttggctagct tcttggaatt cttttggtaa agttccctga
     8761 ccataagttg cataaataaa gtgagtcgtc ccatccttac tcggctctac aattcttcta
     8821 gctattaaac tctgttctga atttgcaaaa ttctccaatt cctgttcaaa tacctcacgc
     8881 gtcggttcct gagtatcttt tttgacacga agtaaagaaa cggaatcata gcttgcatat
     8941 aaatattgtg gcgcacgtaa gacaataatc caagcaagga agaagctgag aaaaaaagtt
     9001 gataataata tgaatagttt cttcatagta gactccttgt aaacaaaatt ccccctgtaa
     9061 tttcttacaa ggggaacgat ttaaatcaat gaacgattag tcataatcac agtaaaatgc
     9121 tacttgttct ccccatttag tccaaatcca tgcataggag gtttttccag caggagcttc
     9181 tcctttatca gaatgaccat tccatctgct cacgatggat gaataatgat attttttacc
     9241 atgatagtaa tttgaaaaag cccaaccacc tcctgaacct tctccatatg tccatactcc
     9301 tccatctgga tattgtacag cagctgatgc agctcccaat aatgtaaaac ttgaaataag
     9361 agctagagca agtaatctat gttttttcgt tttcatttta ttttttcttt caaaaaaagc
     9421 acaccctgag caacaatgca acaaaataaa tcctcctctc tcttttgttg aaaccgcttt
     9481 cttatgtgat aagaataaca tttttattat ttgttgtcaa ggaaaaaatc gaatttttta
     9541 gatattttac tatattatct cta
//
LOCUS       DAVHIU010000067         9513 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVHIU010000067 DAVHIU010000000
VERSION     DAVHIU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.069"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..66
                     /locus_tag="VVN06_001396"
     CDS             <1..66
                     /locus_tag="VVN06_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5562920.1"
                     /translation="EDLSKISEPEKLYAYIAEATA"
     gene            104..406
                     /locus_tag="VVN06_001397"
     CDS             104..406
                     /locus_tag="VVN06_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5562921.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            414..1217
                     /locus_tag="VVN06_001398"
                     /pseudo
     CDS             414..1217
                     /locus_tag="VVN06_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1254..1730
                     /locus_tag="VVN06_001399"
     CDS             1254..1730
                     /locus_tag="VVN06_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU5562922.1"
                     /translation="MPYFIGGHPGFNCPLLDDGVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGEHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1887..2312
                     /gene="lacA"
                     /locus_tag="VVN06_001400"
     CDS             1887..2312
                     /gene="lacA"
                     /locus_tag="VVN06_001400"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU5562923.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFIVATKIKGMVAAEVSDERSAYMTRSHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2343..2858
                     /gene="lacB"
                     /locus_tag="VVN06_001401"
     CDS             2343..2858
                     /gene="lacB"
                     /locus_tag="VVN06_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU5562924.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHVETHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2869..3798
                     /locus_tag="VVN06_001402"
     CDS             2869..3798
                     /locus_tag="VVN06_001402"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU5562925.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGNLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            3800..4780
                     /gene="lacD"
                     /locus_tag="VVN06_001403"
     CDS             3800..4780
                     /gene="lacD"
                     /locus_tag="VVN06_001403"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU5562926.1"
                     /translation="MALTEQKRARLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5014..5850
                     /locus_tag="VVN06_001404"
     CDS             5014..5850
                     /locus_tag="VVN06_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5562927.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGFKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5886..6203
                     /locus_tag="VVN06_001405"
     CDS             5886..6203
                     /locus_tag="VVN06_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5562928.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6203..7894
                     /locus_tag="VVN06_001406"
     CDS             6203..7894
                     /locus_tag="VVN06_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU5562929.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALVFVQEQFD"
     gene            8109..>9513
                     /gene="lacG"
                     /locus_tag="VVN06_001407"
     CDS             8109..>9513
                     /gene="lacG"
                     /locus_tag="VVN06_001407"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU5562930.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLNL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLIDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         3015 a         1728 c         1975 g         2795 t
ORIGIN      
        1 gaagacctat caaaaatttc tgaaccagaa aaactttatg cttacattgc tgaagcaacc
       61 gcttaatctt gtctattaaa aaataaaatc ggaggaaatc caaatgatta aaattcttgc
      121 tgcctgcggt gcaggtgtta actcaagtca ccaaattaaa agtgctctag aagaagaact
      181 ttcaaaccgt ggttatgatg ttcactgtga tgcagtcatg gtgaaagatg taaacgaaga
      241 ccttatgaaa ggttacgata tctttacacc aatcgctgca acagaccttg gttttgaacc
      301 aggtatccca gttatcgaag ctgggccaat cttattctgt atcccagcaa tgagcgttcc
      361 agtatttgac aatattagac ttcctgcgaa acaaaatatg gtatagtagt tctatgaatg
      421 atgaagcaag taaacaacta actgatgcac gatttaagcg tcttgttggt gttcagcgta
      481 ccacttttga agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
      541 gtggacgaaa acctaaatta agcctagaag accttcttat ggccactctt caatagtgcg
      601 agaatatcga acttatgaac aaattgcggc tgattttggt attcacgaaa gcaactttat
      661 ccgtcggagc caatgggttg aagtaactct tgttcaaagt ggtgttacgg tttcaagaac
      721 tcctctcagc tctgaggaca cggtaatgat tgatacgacg gaagtacaaa tcaatcgccc
      781 taaaaaaaga attagcgaat tattctggta aaaagaaatg ccacgctatg aaggctcaag
      841 cgattgtcac aagtcaaggg agaattgttt ctttggatat cactgtgaac tattgtcatg
      901 atatgaagtt gttcaaaatg agttgcagaa atatcggaca agctggtaaa atcttggctg
      961 acagtggtta tcaagggctc atgaagatat atcctcaagt acaaactcca cgtaaatcca
     1021 gcaaactcaa gtcgctaata gctgaagata aagcctataa ccatgcgcta tctaaggaga
     1081 gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt taaaatgttt tcaacaacct
     1141 atcgaaatca tcgtaaacgc ttcggattac gaatgaattt gagtgctggt attatcaatc
     1201 atgaactagg attctagttt tgcaggaagt ctaataaaga aactagcaag gtcatgcctt
     1261 actttattgg tggacatcca ggatttaatt gtcctctact tgatgatgga gtctatgaag
     1321 attattatct agagtctgag aaagaagaga cttgctctgt tccacgtcct ttcccagaaa
     1381 caggtatgtt ggatttccaa gatagaagtc catggctaga gggacaaaaa gaaatagatc
     1441 ttagttatga tcttttcagc aaagatgcag tgactttgga tgaattgcaa tctcgaacaa
     1501 ttgcccttcg ttctcttaaa catgataagg gattgaaagt gcattttgca gaatttccaa
     1561 acctcatcat ctggtcaact ttgaacaagg gacctttcat tacctttgaa ccatggtctg
     1621 gcttgtcaac attccttgaa gaaggagaac atttagaaga taagaagaat gtttgtctct
     1681 tagaagccaa tcaggttgaa gaattagggt ttgagataga agttttataa aaaagtaaaa
     1741 aataaataac aaaaacaaac acaaactatt gacttcttca aacaatagta gtatattatg
     1801 tttgtgagga ctaattgact gttttttaaa gacagccaat acactgctgg aatccagcat
     1861 agaaaaaata aaaaggagta tacaatatgt ctattgttat cggtgcagat gctgcaggtt
     1921 tgagattgaa agaagttgtg aaagacttct tagaaaaaga aaacttccac cttgtggatg
     1981 ttacagctga aggtcaagac tttgttgatg tgactctcgc tgttgctgca gaagtaaaca
     2041 aagaagaaca aaaccttggt atcgtgattg atgcttatgg agctggtcca tttatagttg
     2101 caactaagat caaaggaatg gttgctgcag aagtatctga cgaacgttca gcttatatga
     2161 ctcgtagcca caacaactca cgtatgatca ctatgggggc acaacttgtt ggtgatgaat
     2221 tggctaaaaa catcgctaaa ggatttgtta atggtaaata cgacggtgga cgtcatcaaa
     2281 tccgggttga catgttgaac aaaatgggct aattagatta cagtaagaaa ggtaagttaa
     2341 aaatgagaat tgcaattgga tgcgaccaca tcgtaacaga tgaaaaaatg gcggtttcag
     2401 aatttttgaa atcaaaagga tatgaagtca ttgactttgg tacatatgac catacacgga
     2461 ctcactaccc aatctttggt aaaaaagtag gggaagctgt aactagcggc caagctgatc
     2521 ttggagtatg tatctgtggt actggtgttg gtatcaacaa cgctgtaaat aaagttccag
     2581 gtgttcgctc tgccttggtt cgtgatatga caacagccct ttatgctaaa gaacaattga
     2641 acgccaacgt tatcggtttt ggtggtaaga ttactggtga attgctcatg tgcgatatca
     2701 tcgaagcttt catccatgct gaatacaaac catctgaaga aaacaaaaaa ttgattgcga
     2761 aaattgaaca tgttgaaaca cacaatgctc aacaaacaga tgcaaacttc ttcactgaat
     2821 tccttgaaaa atgggatcgc ggagaatacc acgactaaga ggtgacctat gattttaaca
     2881 gtcacaatga acccatccat tgatatttcc tatcccttgg atgagttgaa gattgatact
     2941 gtcaatcgtg tggtagatgt aaccaaaacg gctggtggta aagggctcaa tgttacacgc
     3001 gtactttcag aatttggcga ttctgttctt gctactggtt tagtaggtgg caaccttggt
     3061 gagtttttgg ttgaacatat cgataatcaa gtaaagaaag atttcttctc aattcaggga
     3121 gaaactcgta actgtatcgc tattctccac ggagacaacc aaacagaagt tcttgaaaaa
     3181 ggtcctgaag tattggaaca ggaaggtcaa gactttttgg aacatttcaa aaaactcttg
     3241 gagtcagttg aagtagtggc catctcaggt agtctgccag ctggccttcc agttgattac
     3301 tatgcgagct tggtagaact tgctaatcaa gctggcaagc ctgtagtctt ggactgctca
     3361 ggtgcagcac ttcaggctgt tcttgaatca ccccataaac caacagtcat caaaccaaat
     3421 aatgaagaat tgtctcagct tcttggaaga gaagtttctg aggatttgga tgaattaaaa
     3481 gaagtacttc aagaaccttt gtttgcaggg attgaatgga ttatcgtttc acttggtgcc
     3541 aacggtactt ttgccaaaca tggtgacact ttctacaagg tagatattcc tagaattcag
     3601 gtggtaaatc ctgttggatc tggagactct actgtggcag gaatttcttc aggacttctt
     3661 cacaaagaat cggatgcaga attactcatc aaggcaaatg tccttggtat gctcaatgct
     3721 caagaaaaaa tgactggtca tgtcaacatg gccaactatc aagttctata tgatcaatta
     3781 atagtaaaag aggtataaaa tggctttaac agaacaaaaa cgtgcacgct tagaaaaact
     3841 ttctgatgaa aatggtatca tctcagctct tgcatttgac caacgtggtg ctttgaaacg
     3901 cctcatggct caacaccaaa cagaagaacc aactgtggct caaatggaag aactgaaagt
     3961 cttggtagca gatgaattga ctaaatacgc ttcatcaatg cttcttgacc ctgagtatgg
     4021 acttccagca actaaagctc ttgatgaaaa agctggtctt ctccttgctt atgaaaaaac
     4081 aggttatgac acaacaagta caaaacgctt gccagactgc ttggatgttt ggtctgcaaa
     4141 acgtattaaa gaagagggtg cagatgcagt taaattcttg ctttactatg atgtagatag
     4201 ttcagacgaa ctcaaccaag aaaaacaagc ttatatcgag cgtatcggtt ctgagtgtgt
     4261 ggctgaagat atcccattct tccttgaaat ccttgcttac gatgaaaaaa ttgcagacgc
     4321 aggttctgta gaatatgcga aagtaaaacc acacaaagtt atcggtgcta tgaaagtctt
     4381 ttcagaccca cgctttaaca ttgatgtctt gaaagttgaa gttcctgtta acattaaata
     4441 tgttgaaggc ttcgctgaag gtgaagtggt ttacacacgt gaagaagcag cagccttctt
     4501 caaagcgcaa gatgaagcaa cgaacttgcc atacatttac ttgagtgctg gtgtatcagc
     4561 taaactcttc caagatactc ttgtatttgc tcatgaatca ggtgcaaact ttaacggagt
     4621 tctttgtggc cgtgctacat gggcaggatc agttgaagct tacatcaaag atggtgaagc
     4681 agcagctcgc gaatggcttc gcacaactgg atttgaaaac attgatgagc tcaataaagt
     4741 tcttcaaaca acagcgactt catggaaaga acgtgtgtaa gaaagtcctc ctagtttagg
     4801 aacatgaatc taaaaaaatt taaaaaaagt tgtatgtaaa ggtttacaaa ataacttact
     4861 tgtgctatac ttaaatcaca agttaataca aggtgagtgt tactaagtaa tattaggcat
     4921 gatcacaggt gaattagaaa tcagctgatt ttctagttca tttgtggtca ttttttgtac
     4981 tcatatactt ttaagatata aaaggaggtt gacatgtatc gaattctaaa tccaatgaat
     5041 cacaatgtct cgcttgtcag aaatgataag ggagaagagg tgattgtaat tggtaagggg
     5101 attgcattcg gaaagaagaa gggggatttg attgctgaaa atcaggttga gaaaatcttt
     5161 cggatgaaga ccgaagagtc cagagaaaac tttatggctc ttctcaaaga tgttccgctt
     5221 gattttatca cagtgaccta tgaaatcatt gataagctat caaagaaata tcattatccg
     5281 attcaagagt atctctatgt aaccttgaca gatcatattt actgttctta tcaagctcta
     5341 actcaaggaa ggtacaagga tagtaatctg ccagatattt ccgctaagta tcctgtcgct
     5401 tttcaaatcg caaatgaagc ttttgaaatt taccgtcaga agctagcaga tcattttcct
     5461 gaggacgaaa ttattcggat tgcttatcat ttcattaatg ctgaaggtga aaatgaagtg
     5521 gaacttgtgg agtcgattga taagaggaaa gaaattctca ggaatgttga agaagtttta
     5581 acggactatg caattcaacg aactaaaaag aataaccatt tctatgatcg ctttatgatc
     5641 catttgaatt atttcttgga ttatttagac agatctagag atgataacca atcacttctg
     5701 gatatggaag atcatattaa acaatcctat ccaaaagcct tcgagattgg tttcaagatc
     5761 tatgatgtga ttacgcaaca tacgggtctt gatttgtata aaagtgaacg agtttatcta
     5821 gttctacata tccaacgttt attgtcataa aaatttattt aaaactatat aaggagaatt
     5881 ctatcatgaa tagagaagaa gtaacattgt taggttttga aatcgtagcc tatgctggcg
     5941 atgctcgttc aaaactattg gaagccttga aggctgctga agctggtgat tttgaaaaag
     6001 cggacgctct ggtagaggaa gctggtagct gtattgcaga ggctcaccac gcgcaaacaa
     6061 gtctattgac taaggaagct tcaggtgagg acttggctta tagtgtaacc atgatgcatg
     6121 gccaagacca cttaatgaca actatcttgt taaaagattt gatgcatcat ttaattgaac
     6181 tctacaagag aggagttaaa taatgaataa actaattgca tttatcgaga aaggaaagcc
     6241 tttctttgaa aaactatctc gtaatatcta tcttcgtgct attcgtgatg gtttcattgc
     6301 aggtatgcct gttattctct tctcaagtat ctttatcttg attgcctttg taccaaactc
     6361 atggggcttt aaatggtctg atgaagttgt agcctttctg atgaaacctt atagctattc
     6421 tatgggtatt ctggctctct tggtagctgg tacaacagct aagtcattga ctgactcagt
     6481 aaaccggagc atggaaaaaa ccaatcaaat caactatatg tcaacattgt tggcagcaat
     6541 tgttggtttg ttgatgttgg cagctgatcc tatcgaaagt ggtctagcta ctggattctt
     6601 ggggacaaaa ggtttgcttt cagccttcct tgctgccttt gttactgtag ccatctataa
     6661 ggtttgtgtt aagaacaacg tcactattcg tatgcctgac gaagttccac caaatatctc
     6721 acaagtcttt aaagatgtga ttccattcac tctatctgtt gtttctcttt atgctcttga
     6781 cttattagca cgttattttg ttggttctag tgtggcagaa tcaatcggta aattcttcgc
     6841 accactcttc tcagcagcag acggatacct tggtattacc attatctttg gtgcctttgc
     6901 cttcttctgg tttgttggga ttcatggtcc atctatcgtt gaaccagcta tcgcagctat
     6961 tacctatgcc aatgccgaag tcaacttgaa ccttctccaa caagggatgc atgcagacaa
     7021 gattcttact tctggtacac aaatgtttat cgttaccatg ggtggtacag gtgcgacatt
     7081 ggtcgttcca tttatgttca tgtggttgac aaaatcgaaa cgtaaccgtg caatcggacg
     7141 tgcttcagta gttcctacct tcttcggtgt aaatgaacca atcttgtttg gtgcacctct
     7201 tgttttgaat ccaatcttct tcattccatt tatctttgct ccaattgcaa acgtatggat
     7261 tttcaaattc tttattgaaa ctcttggaat gaactcattc actgctaatc taccatggac
     7321 aactccagct ccactaggtc tagttcttgg aactaacttc caagtgctat cattcattct
     7381 tgctgccctt ctaattgtgg ttgacgtagt tatttattac ccattcctta aagtctatga
     7441 tgaacaaatt cttgaagaag aacgttcagg taagtctaat gatgaattga aagaaaaagt
     7501 tgctgcaaac ttcaacactg caaaagcgga tgctattctt gaaaaagcgg gtgtcgatgc
     7561 agcacaaaat accatcactg aagaaacaaa tgtcctcgtt ctctgtgctg gtggaggtac
     7621 aagtggactc cttgcaaatg ctttgaataa ggcagcagca gaatacaatg tccctgtgaa
     7681 agcagcagca ggcggctatg gtgctcaccg tgaaatgtta ccagagttta atctagttat
     7741 ccttgcccct caagttgctt caaactttga agatatgaag gcagaaacag ataagctcgg
     7801 tattaaactt gctaagacag aaggcgctca atacatcaaa ttaactcgtg atggaaaagg
     7861 tgccctagta ttcgtacaag agcaattcga ttaaggctag gaactgtgaa acagtctccc
     7921 atcgttacgg aaatcgctat ggcgaatttc ctaatcgcct tattaattcg tcggtaaaaa
     7981 gatatcgttt ttacctcctc atgtcacaat tcagtgactt ggtacaagaa gtgagatgga
     8041 gaagcatggc tcactgactc ctctcctctc acttttactt tatttaaatc aagaaatagg
     8101 tgaaaaaaat gacaaaaaca cttccaaaag actttatttt cggtggcgca acagctgctt
     8161 atcaagcaga aggtgctaca catactgatg gtaaaggacc agtagcgtgg gataaatatc
     8221 ttgaagataa ctactggtac actgcagaac cagccagtga tttttacaat cgatatccag
     8281 ttgacctcaa gctagcagaa gagtatggtg tcaatggcat tcgaatttct attgcctggt
     8341 cacgtatttt cccgactggt tatggtcaag taaatgctaa aggtgttgag ttttatcata
     8401 atctatttgc ggagtgccac aaacgtcatg ttgagccctt tgtaactctt catcactttg
     8461 acacgccaga agctctccac tcaaatggcg acttcttaaa ccgtgaaaac attgaacact
     8521 ttgtagacta cgcggctttc tgttttgaag aatttccaga agtaaactat tggacaacct
     8581 tcaatgaaat tggaccaatc ggtgatggtc aatatttagt tggtaaattc cctccaggaa
     8641 ttcagtacga ccttgccaaa gtcttccaat cgcaccacaa tatgatggtg tctcatgcac
     8701 gcgcggtaaa attgtacaaa gagaaaggct ataaagggga aattggtgtt gttcacgccc
     8761 tgcctactaa atatcctcta gatcatgaaa atccagcaga tgttcgtgca gctgagttgg
     8821 aagatatcat ccacaataaa ttcatcttag acgcaactta tctaggtcgc tattcagctg
     8881 aaaccatgga aggtgtcaac catatcttat cagtcaatgg tggtagtttg aatcttcgtg
     8941 aagaagattt tacagcatta gaagctgcaa aagacttgaa tgatttccta ggaatcaact
     9001 actatatgag tgactggatg gaagcctttg atggagaaac tgaaattatc cataatggta
     9061 aaggtgaaaa aggaagctct aagtatcaaa tcaaaggtat tggtcgtcgt gtagctcctg
     9121 actatgtatc acgcacggat tgggattgga ttatctaccc tcaaggtttg tatgaccaaa
     9181 tcatgcgtgt gaagaaagat tatcctaact acaagaaaat ttatatcact gaaaatggtc
     9241 tcggctataa agatgagttc gttgataaca ctgtttacga tgatggtcgt attgattacg
     9301 tgaaacaaca cttggagatc ttgtctgatg cgattgcaga tggagctaat gtaaaaggtt
     9361 acttcatttg gtcattaata gatgtcttct catggtcaaa cggttatgag aaacgttatg
     9421 gtctcttcta cgtagatttt gaaactcaag aacgttatcc taagaaatca gctcactggt
     9481 acaaaaaagt agcggaaact cagattatag act
//
LOCUS       DAVHIU010000068         9499 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVHIU010000068 DAVHIU010000000
VERSION     DAVHIU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.070"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            17..1195
                     /locus_tag="VVN06_001408"
     CDS             17..1195
                     /locus_tag="VVN06_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU5562931.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            1419..2198
                     /gene="rpsB"
                     /locus_tag="VVN06_001409"
     CDS             1419..2198
                     /gene="rpsB"
                     /locus_tag="VVN06_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5562932.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            2277..3317
                     /locus_tag="VVN06_001410"
     CDS             2277..3317
                     /locus_tag="VVN06_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU5562933.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            3563..3886
                     /gene="tnpB"
                     /locus_tag="VVN06_001411"
     CDS             3563..3886
                     /gene="tnpB"
                     /locus_tag="VVN06_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU5562934.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(4027..4335)
                     /locus_tag="VVN06_001412"
                     /pseudo
     CDS             complement(4027..4335)
                     /locus_tag="VVN06_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4326..4724
                     /locus_tag="VVN06_001413"
     CDS             4326..4724
                     /locus_tag="VVN06_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5562935.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(4921..5841)
                     /gene="cysK"
                     /locus_tag="VVN06_001414"
     CDS             complement(4921..5841)
                     /gene="cysK"
                     /locus_tag="VVN06_001414"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU5562936.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(6126..6761)
                     /locus_tag="VVN06_001415"
     CDS             complement(6126..6761)
                     /locus_tag="VVN06_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU5562937.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYTSSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            6817..8115
                     /locus_tag="VVN06_001416"
     CDS             6817..8115
                     /locus_tag="VVN06_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5562938.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            8112..8774
                     /locus_tag="VVN06_001417"
     CDS             8112..8774
                     /locus_tag="VVN06_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU5562939.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            8854..9402
                     /gene="raiA"
                     /locus_tag="VVN06_001418"
     CDS             8854..9402
                     /gene="raiA"
                     /locus_tag="VVN06_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5562940.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
BASE COUNT         3063 a         1690 c         1964 g         2782 t
ORIGIN      
        1 ggagtatcta cgtaatatga agaaaaaaat cttagcgtca cttttattaa gtacagtaat
       61 ggtttctcaa gtagctgttt taacaactgc gcatgcagaa acgactgatg acaaaattgc
      121 tgctcaagat aataaaatta gtaacttaac agcacaacaa caagaagccc aaaaacaagt
      181 tgatcaaatt caggagcaag tatcagctat tcaagctgag cagtctaact tgcaagctga
      241 aaatgataga ttacaagcag aatctaagaa actcgagggt gagattacag aactttctaa
      301 gaacattgtt tctcgtaacc aatcgttgga aaaacaagct cgtagtgctc aaacaaatgg
      361 agccgtaact agctatatca ataccattgt aaactcaaaa tcaattacag aagctatttc
      421 acgtgttgct gcaatgagtg aaatcgtatc tgcaaacaac aaaatgttag aacaacaaaa
      481 ggcagataaa aaagctattt ctgaaaaaca agtagcaaat aatgatgcta tcaatactgt
      541 aattgctaat caacaaaaat tggctgatga tgctcaagca ttgactacga aacaggcaga
      601 actaaaagct gctgaattaa gtcttgctgc tgagaaagcg acagctgaag gggaaaaagc
      661 aagtctatta gagcaaaaag cagcagctga ggcagaggct cgtgcagctg cggtagcaga
      721 agcagcttat aaagaaaaac gagctagcca acaacaatca gtacttgctt cagcaaacac
      781 taacttaaca gctcaagtgc aagcagtatc tgaatctgca gcagcacctg tccgtgcaaa
      841 agttcgtcca acatacagta caaacgcttc aagttatcca attggagaat gtacatgggg
      901 agtaaaaaca ttggcacctt gggctggaga ctactggggt aatggagcac agtgggctac
      961 aagtgcagca gcagcaggtt tccgtacagg ttcaacacct caagttggag caattgcatg
     1021 ttggaatgat ggtggatatg gtcacgtagc ggttgttaca gctgttgaat caacaacacg
     1081 tatccaagta tcagaatcaa attatgcagg taatcgtaca attggaaatc accgtggatg
     1141 gttcaatcca acaacaactt ctgaaggttt tgttacatat atttatgcag attaatttac
     1201 agagggactc gaatagagcc ctcttttcag gttttaccgt gacaatccct attaaaaatt
     1261 atatcaaaat agcttgaaaa tattggaaaa gtatggtaga atgaaaattg tcgtgtgaac
     1321 gataatactc attcttgatg aattgtgaag cagttgccct tgggtcgttt tgcgagttga
     1381 agtcaagaag aggaaaaaaa caaaaaggag aaatactcat ggcagtaatt tcaatgaaac
     1441 aacttcttga ggctggtgta cactttggtc accaaactcg tcgctggaat cctaagatgg
     1501 ctaagtacat ctttactgaa cgtaacggaa tccacgttat cgacttgcaa caaactgtaa
     1561 aatacgctga ccaagcatac gacttcatgc gtgatgcagc agctaacgat gcagttgtat
     1621 tgttcgttgg tactaagaaa caagcagctg atgcagttgc tgaagaagca gtacgttcag
     1681 gtcaatactt catcaaccac cgttggttgg gtggaactct tacaaactgg ggaacaatcc
     1741 aaaaacgtat cgctcgtttg aaagaaatta aacgtatgga agaagatgga actttcgaag
     1801 ttcttcctaa gaaagaagtt gcacttctta acaaacaacg tgcgcgtctt gaaaaattct
     1861 tgggcggtat cgaagatatg cctcgtatcc cagatgtgat gtacgtagtt gacccacata
     1921 aagagcaaat cgctgttaaa gaagctaaaa aattgggaat cccagttgta gcgatggttg
     1981 acaccaatac tgatccagat gatatcgatg taatcatccc agctaacgat gacgctatcc
     2041 gtgctgttaa attgatcaca gctaaattgg ctgacgctat tatcgaagga cgtcaaggtg
     2101 aggatgcagt agcagttgaa gcagaatttg cagcttcaga aactcaagca gattcaattg
     2161 aagaaatcgt tgaagttgta gaaggtgaca acgcttaatt tatacaaata gtaattacct
     2221 aggagggcgg ggcttagccc ggctctccta ttttcaaaaa atataggaga attaaaatgg
     2281 cagaaattac agctaaactt gtaaaagagt tgcgtgaaaa atctggtgcc ggtgttatgg
     2341 acgctaaaaa agcgcttgta gaaacagacg gtgacatcga aaaagcgatt gaattgcttc
     2401 gtgaaaaagg tatggctaag gcagctaaga aagctgaccg tgttgctgca gaaggtttga
     2461 ctggtgttta tgttaacggt aatgttgcag cagttattga agtaaacgct gaaactgact
     2521 tcgttgcaaa aaacgctcaa ttcgttgaat tggtaaatac tacagctaaa gtcattgctg
     2581 aaggaaaacc tgctaacaat gaagaagctc ttgctttgat aatgccttca ggtgaaactc
     2641 ttgaagctgc atacgtatct gcaacagcaa ctatcggaga gaaaatctca ttccgtcgct
     2701 ttgcattgat tgaaaaaaca gacgcacaac actttggagc ataccaacat aacggtggac
     2761 gtatcggtgt tatttcagtt gttgaaggtg gagacgaagc acttgctaaa caattgtcaa
     2821 tgcacatcgc agcgatgaaa ccaacagttc tttcttacaa agaattggat gagcaattcg
     2881 ttaaagatga gttggcacaa ttgaatcacg ttatcgacca agacaacgaa agccgtgcaa
     2941 tggttaataa gccagctctt ccacacttga agtatggatc aaaagctcaa ttaactgatg
     3001 atgttattgc tcaagctgaa gctgacatca aagctgaatt ggctgcagaa ggcaaaccag
     3061 aaaaaatctg ggacaaaatt attccaggta aaatggatcg cttcatgctt gataacacta
     3121 aagttgacca agcttacaca cttcttgcac aagtttacat catggatgac agcaagacag
     3181 ttgaagcata ccttgaatca gttaacgctt cggtagttga gtttgctcgc tttgaagttg
     3241 gtgaaggtat cgagaaagct gcaaacgact tcgaagctga agttgcagct acaatggcag
     3301 cagccttgaa taactaataa tagaaaggaa gcaatttgct tccttttttg tatagggctt
     3361 atatttttcc tgaaattgtt tttcccccag tctcactcga ttagaataat taaaacaaaa
     3421 agccctataa taagggctaa gtgcatttag gagactatac ttcaaattca tagtaagcgc
     3481 gtcataacaa ggtatctatc ctaattcccc aacttccagt atactagata aaaaataatt
     3541 atcggaggaa aggtattgta ctatgatgat tcaactaagt gatttaggtc aagttcacat
     3601 tgtttgtggc aagacagata tgaggcaggg aatagactca ttagcctatg tagttaaaac
     3661 ccactttgaa ttggattctt tctccggtca agtctttctc ttttgtggtg gacgtaaaga
     3721 ccgctttaaa gtcctttact gggatggtca aggattttgg ctactatata aacgctttga
     3781 gaacggaaaa ctgacttggc taagtacaga aaaggatgtc aaagctctca catctgaaca
     3841 agtagactgg cttatgaagg gcttttctat cactccccaa atatagtggt ttgaaactag
     3901 aatagtacac ctctgcttct aaaacattgt tagaaatcga tttgactgtc ctgttcttat
     3961 ttcattttac tataaattca tcagaaagtc gtgatttcta ttgaaatgag gactttcttt
     4021 ttatactcat ctgttttcac aaagcattct agtccatctc cgattaacga tggactttat
     4081 cacctccttc tccagtcctt gtataacatc ttggagttga ttcatgacat cttccaaaat
     4141 ttgaaaggct ttattcttaa atccacgttt acgaatctct ttccacactt gttcaatggg
     4201 gttcatctct ggtgtgtatg gaggaataaa tgcaaagcca atattagtcg gaatctttaa
     4261 ggtacttgat ttatgccata tagcattgtc cataacgagt aaaagataat catctggata
     4321 agcttgtgaa atctcctatt cctaaagccc ctttagcgca taactttggc tcagcttcta
     4381 ttatcgctca caccatccat cagaagttta atctgaaggt acccaattat cgccaagaag
     4441 aagattgggc taggatgggt ttaccaatca cacgtaagga aatctctaat cggcatatca
     4501 agacgagtca atactatttg gagccccttt ataacctctt gcgagagaga ctattgactc
     4561 agcccttact tcatgcggat gaaacttctt atagggtgct agagagtgat agtcagctga
     4621 cttactattg gacttttttg tctgggaaag ctgagaaaca agggattacg ctttaccatc
     4681 atgtcctaat cgatttgttc atatcttatt tcaatccatt ataaatagtg agaaatatct
     4741 atcctatctt ctagaatgtc ttccaaacgt ggaaactctc gtaaacaaag aggttttaga
     4801 ggcctattta ccatggacta aagttgtaca agaaaagtgc aaataagaaa tctccagatt
     4861 aggaactatc cgtgagttct ctagtctgga gatttttcaa tagacttcgt tattggacgg
     4921 ttacaattca taaagtgctg tagagagata acgttcaccg ttatctggtg ctagggcaag
     4981 gactttttta cctgtaccta attttttggc aacctcgatg gctccgtaga tagctgcagc
     5041 tgaggaaatc cctacaagga agccttcttt tccaccaatt tcacgtccga gtgcaagagc
     5101 gtcatctgat gttacacgaa cgataccatc ataggcttta gtatcaagtg tatcaggaat
     5161 aaatccagct gagatacctt gaattttgtg aggaccaggt ttttcaccag atagaatagc
     5221 agattcatct gcttctactg caaaaacttg aatgttagaa ttttctgatt tgagtgcatg
     5281 agaaacacca gaaatcgttc caccagtacc tactccagca acaaaggcat ctaatccatc
     5341 tttaccgaaa gcagctagta tctcagctcc tgttgttctt tcgtgtactt ctggattagc
     5401 tggattgtca aattgaagag gaaggaaacc atcacgttca gcagcgattt cttgagcctt
     5461 agcaatagca cctttcattc cctcgctacc aggagttagg acgagttcag caccataagc
     5521 ttggataatt ttacgtcgtt ctacactcat agtttcaggc ataacgatga cgactttata
     5581 ccctttagca gcacctaccc atgaaagtcc aataccggtg tttccacttg ttgcttcaac
     5641 aatagtagaa ccaagtttca gaataccatc ttgttcagct ttttcaatca tgctaagggc
     5701 aatacggtct tttacagatg aaccaggatt aaatgcttca agctttatat agacgtctgc
     5761 agcaccttct ggcacgatgt tgttaagttt aacaatcggt gtttgaccga ttaattcagt
     5821 aatgttgtta taaatagaca tatgaatacc tctttgtata agatatcttt agtataacgc
     5881 tatccatacc atttgtaaaa tatagaaatc ctatcgaagc gataggattt ctatatatca
     5941 tagatcaaag tcattaattt tcaagtgatt gcgttcagtt aattctagta aataggtgtg
     6001 aaggaaaacg atatcaggtt tcgtttgaat tgcttcattt tctagtatat tttggtacgc
     6061 atctttttat tttgctcgct aaagtagttg tgagaatgtc aacgaaacgc tgtattattc
     6121 attgtttaca ctaagtttac aggaacttca acctctcgta aaccttggtc agttaaagtg
     6181 acttttccat taaaaaactc cacaagtgca gctttaatag tttctttttc ttctttatca
     6241 acataaatca tcgtatcgac ttgatctgta aagtttgtat ccagctccat gagatcatgt
     6301 tctttaagga agttactgta ctcttggtac tgagcataag acatttgaat agcaatgcca
     6361 gcctgttctt ttatttcaat aataccaatt tctttgacag ctaaggcgac actgctggtg
     6421 taagcacgaa ttagtcctcc agcgcctagt ttaataccac caaagtagcg tgtcacgacc
     6481 acacagacat tggtgagatt gtgattttct agtaccccaa gcatgggaac accagcagta
     6541 ccactaggct caccatcatc acttgtacgt ttaatttcac tacgttctcc aataataaag
     6601 gcagagcagt tatgtgtcgc tttgtagtgt tcttttttga tggcagtaat gaagtcacga
     6661 gcctcttctt cgctataaac acgcttggca tggcagataa agcgagattt tttgatttct
     6721 tcttggactt ggccgtcctc tttaattgtc ctaaattcca tgatttaatt gtactaaaaa
     6781 atcatctaaa gtgctagttt ttacgaataa agaagtatga aagtaaattt agattatctc
     6841 ggtcgtttat ttactgagaa tgaattaaca gaagaagaac gtcagttggc ggagaaactt
     6901 ccagcaatga gaaaggagaa ggggaaactt ttctgtcaac gttgtaatag tactattcta
     6961 gaagaatggt atttgcccat cggtgcttac tattgtcgag agtgcttgct gatgaagcga
     7021 gtcagaagtg atcaaacttt atactatttt ccgcaggagg attttccgaa gcaagatgtt
     7081 ctcaaatggc gcggccaatt aactcctttt caagagaagg tgtcagaggg actgcttcaa
     7141 gcagtagaca agcaaaagcc aaccttagtt catgcggtaa caggagctgg aaagacagaa
     7201 atgatttatc aagtagtggc taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt
     7261 cctcgcatag atgtttgttt ggagctgtac aagcgcctgc aacaggattt ttcttgcggg
     7321 atagctttgc tacatggaga atcggaacct tattttcgaa caccactagt tgttgcaaca
     7381 acccatcagt tattgaagtt ttatcaagct tttgatttgc tgatagtgga tgaagtagat
     7441 gcttttcctt atgttgataa tcccacgctt taccatgctg tcaagaatag tgtaaaggag
     7501 aatggattga gaatcttttt aacagcgact tcgaccaatg agttagataa aaaggtccgt
     7561 ttaggagaac taaaaagact gaatttaccg agacggtttc atggaaatcc gttgattatt
     7621 ccaaaaccaa tttggttatc ggattttaat cgctacttag acaagaatcg tttgtcacca
     7681 aagttaaagt cctatattga gaagcagaga aagacagctt atccgttact catttttgct
     7741 tcagaaatta agaaagggga gcagttagaa gaaatcttac aggagcaatt tccaaatgag
     7801 aaaattggct ttgtatcttc tgtaacagag gatcgattag agcaagtaca agcttttcga
     7861 gatggagaac tgacaatact tatcagtacg acaatcttgg agcgtggagt taccttccct
     7921 tgtgtggatg ttttcgtagt agaagccaat catcgtttgt ttaccaagtc tagtttgatt
     7981 cagattggtg gacgagttgg acgaagcatg gatagaccga caggagattt gcttttcttc
     8041 catgatgggt taaatgcttc aatcaagaag gcgattaagg aaattcagat gatgaataag
     8101 gaggctggtc tatgaagtgc ttgttatgtg ggcagactat gaagactgtt ttaactttta
     8161 gtagtctctt acttctgagg aatgatgact cttgtctttg ttcagactgt gattctactt
     8221 ttgaaagaat tggggaagag aactgtccaa attgtatgaa aacagagttg tcaacaaagt
     8281 gtcaagattg tcaattttgg tgtaaagaag gagttggagt cagtcataga gcgattttta
     8341 cttacaatca agctatgaag gattttttca gtcggtataa gtttgatgga gacttcctgt
     8401 taagaaaagt tttcgcttca tttttaagtg aggagttgaa aaagtacaaa gagtatcaat
     8461 ttgttgtaat tcccctaagt cctgatagat atgctaatag aggatttaat caggttgagg
     8521 gcttggtaga ggcagcaggc tttgagtatc tggatttatt agagaaaaga gaagagagag
     8581 ccagttcttc taaaaatcgt tcagagcgct tggggacaga acttcctttc tttattaaaa
     8641 gtggagtcac tattcctaaa aaaatcctac ttatagatga tatctatact acaggagcaa
     8701 ctataaatcg tgttaagaaa ctgttggaag aagctggtgc taaggatgta aaaacatttt
     8761 cccttgtaag atgaggaaaa aatttgcaaa aatagaatga aagcgttata ataaaatcat
     8821 aaaaacaaaa atgtttagaa agaaggtact catatgatta aatatagtat ccgtggtgaa
     8881 aacctagaag taacagaagc aattcgtgat tatgtagttt ctaaactcga aaagatcgaa
     8941 aagtacttcc aaccagaaca agagttggat gcccgaatta acttaaaagt ttatcgtgaa
     9001 aaaacggcta aagtggaagt aacgattccg cttggatcta ttactctccg cgcagaagat
     9061 gtatctcaag atatgtatgg ttcaattgac cttgtaactg ataaaattga acgtcagatt
     9121 cgtaaaaata aaacaaaaat cgagcgtaaa aataaaaata aggtagcaac tggtcaatta
     9181 tttacagatg ctttggtgga agattcaaat attgtccagt ctaaagttgt tcgttcaaaa
     9241 caaattgatt taaaaccaat ggatttggaa gaagcaattc tacaaatgga tttattgggg
     9301 catgatttct ttatctatgt ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt
     9361 gaggatggcg aaattggttt gttagaggtt aaagaatctt aatttcaata tgtgtaaagg
     9421 taggtttact gaattgtaaa ctaccttttt cttataattg atagaattac tgattacact
     9481 tttaaaaagt cgctttttg
//
LOCUS       DAVHIU010000069         9478 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVHIU010000069 DAVHIU010000000
VERSION     DAVHIU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.071"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            54..134
                     /locus_tag="VVN06_001419"
                     /pseudo
     CDS             54..134
                     /locus_tag="VVN06_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(293..2116)
                     /gene="lepA"
                     /locus_tag="VVN06_001420"
     CDS             complement(293..2116)
                     /gene="lepA"
                     /locus_tag="VVN06_001420"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU5562941.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(2195..2923)
                     /locus_tag="VVN06_001421"
     CDS             complement(2195..2923)
                     /locus_tag="VVN06_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU5562942.1"
                     /translation="MTDYYVIGDVHGKAEMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            complement(2925..4592)
                     /gene="recN"
                     /locus_tag="VVN06_001422"
     CDS             complement(2925..4592)
                     /gene="recN"
                     /locus_tag="VVN06_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5562943.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            complement(4599..5030)
                     /locus_tag="VVN06_001423"
     CDS             complement(4599..5030)
                     /locus_tag="VVN06_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5562944.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            complement(5023..5838)
                     /locus_tag="VVN06_001424"
     CDS             complement(5023..5838)
                     /locus_tag="VVN06_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU5562945.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            complement(5831..6706)
                     /locus_tag="VVN06_001425"
     CDS             complement(5831..6706)
                     /locus_tag="VVN06_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5562946.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            complement(6703..6915)
                     /locus_tag="VVN06_001426"
     CDS             complement(6703..6915)
                     /locus_tag="VVN06_001426"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5562947.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            complement(6893..8233)
                     /locus_tag="VVN06_001427"
     CDS             complement(6893..8233)
                     /locus_tag="VVN06_001427"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5562948.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGNYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            complement(8361..8999)
                     /gene="udk"
                     /locus_tag="VVN06_001428"
     CDS             complement(8361..8999)
                     /gene="udk"
                     /locus_tag="VVN06_001428"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU5562949.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            complement(9107..9405)
                     /locus_tag="VVN06_001429"
                     /pseudo
     CDS             complement(9107..9405)
                     /locus_tag="VVN06_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
BASE COUNT         2670 a         2306 c         1729 g         2773 t
ORIGIN      
        1 gttatataaa aataaacaca caaacaaata ctccaagcat ttttctgttc taatactcaa
       61 tgaaaatcaa agagcaaact aggaagctag ccgcaggtta ctcaaaacac agttttgagg
      121 ttgtagatga aactgacgaa gtcagtaacc atacatacgg taaggcgacg ctgacgtggt
      181 ttgaagagat tttcgaagag tataaaaact aaaaaagcag accatctaag cctgctttac
      241 tattgattct tatataaatt tcctgtgaac aaggaaaggc atttctgata acttattctt
      301 catccatact caagacgctg aggaaggctt cttgcggaac ttcaactgat ccgatggatt
      361 tcatgcgttt cttaccagct ttttgttttt caaggagttt acgcttacga gaaacgtcac
      421 caccataaca tttagcaagt acgttcttac gaagggcctt gatatcagta cgagcgacaa
      481 tcttgtgtcc aatagccgct tggattggaa cttcaaattg ttggcgaggg atgattttct
      541 tgagtttatc aacgatgagt ttcccacgtt cgtaggcaaa gtccttgtga acgataaagc
      601 tgagggcatc caccttatct ccattgagaa ggatatccat tttcaccagc ttagatgggc
      661 gatattctga taattcgtag tcaaagcttg cataaccacg tgtcgaagac ttaagtttat
      721 caaagaagtc aaagacaatt tcagcaagag gaatttgata gataacattg acacggttat
      781 catcaatata gtccatagtc acaaagtccc cacgcttacg ctgagctagc tccattactg
      841 ctccgacgaa ctcctgtggt accatgattt gcgccttgac ataaggctct tcaatggtcg
      901 caatcttagt tgggtctgga aactcagatg ggttagacac atccatagac tcaccgtcgg
      961 tcaaattaac tttgtaaata acagacggag ctgtcatgat gaggtcaata ttgaactcac
     1021 gctctaaacg ttcctgaata acatccatat ggagaagtcc aagaaatcca caacggaaac
     1081 caaatccaag tgcctgagat gtttctggtt caaactgaag actagcatca ttcagttgca
     1141 atttttcaag cgcttcacgc aggtcattgt acttgtttga ttcgattggg tagagacccg
     1201 caaagaccat aggattcatc tgcttataac catgtaatgg ttctgccgca ggattggttg
     1261 ccaaggtaac ggtatcaccc acacgagtat cctgaaccgt cttgatagac gccgcaatgt
     1321 aaccaacatc accagtcgca aggaaatcac gaccaaccgc ttttggtgta aaaataccga
     1381 cttcggccac atcaaaggtc ttactattgc tcatgagctg aatcttatca ccaggtttga
     1441 ccactccgtc catgacacgc acttggagga taaccccacg gtaagcatcg taaacagagt
     1501 cgaaaatcaa ggccttaagt ggcgccgtca catcacccgt tggtgctggt actttttcta
     1561 caatttgctc gaggatttct tcaatcccaa taccagcctt ggcagaagcc aaaactgctt
     1621 cactggcatc caaaccaatc acatcttcaa tctctgtacg cacgcgctcc ggatctgcag
     1681 ccggcaggtc aattttatta atgataggca tgatttccaa atcattatcc aaagccagat
     1741 aaacgttggc aagagtttga gcctcaattc cttgagccgc atcgaccacc aaaatagcac
     1801 cctcacaggc agctagcgaa cgtgaaactt cataggtaaa gtcaacgtgc cctggtgtgt
     1861 caatcaagtg gaaaatataa gtttccccat cttttgcagt gtaattcaac tcgatggcat
     1921 tcaacttaat agtaattcca cgttcccgct ctagatccat gctatccaaa agctgggcct
     1981 gcatttcacg acttgaaacc gtctctgttt tttccaaaat gcggtctgct agagttgatt
     2041 tcccgtggtc aatatgggcg ataatagaga agttacggat cttcccctgt cgttttttca
     2101 attcttctaa gttcatgatt ctcttccttt cagggtatct atttattata aattgttttt
     2161 gatattttga caagaccata ccctgctagg agtactaatc ttcagcgaca aagccgtcat
     2221 tttcgataaa gtggtgttct gtcattcctt ggtctgtaaa gacaatcccg tgaaggacac
     2281 caccataaac agctcctcca tccattccaa tcttgccatc ttctgtagtc caaagctcag
     2341 atgtaccgcg ttcttgctgt aacaaaccat agaccggtgt atgaccgaag acaatggttt
     2401 ttccagtatg attttcagct ccgtggaatg gttttctaag ccatactttt ttataatctg
     2461 ttgtttcatg ccagtcgtcc aaagtcaaat caatacctgc gtgaacaaat atatacttgt
     2521 ctgtctctac tacaaatggc atttgacgaa tgaattcgac caagtctgac gcttcagcgg
     2581 caacccgctt ggcatcttct actccatcaa ctggtgcatc caagggacga cctaggatag
     2641 agttaatggt tgtatctcca ccattgcgac tataatggtc ataactttct tctgggtcat
     2701 ctagccaagt caaaaacata tactcgtggt ttccggacaa acagatagcc ccttgattgt
     2761 ccaccaagtc cttgaccatt tcaagaacac ggtgactatc ctcacctctg tcaatcaaat
     2821 cacctagaaa gagcaactgg ggctgaccat cccaggtttt gagaaggtct tccagcatct
     2881 cagcttttcc gtgaacatct ccaattacat aataatctgt catcttattt ctccctgttt
     2941 ctcaacaatt ctctggcttg cgtcagggct gcttctgtca catcatcacc tgccaacatc
     3001 ttggcaactt cctccactcg ctcttcgacc gtcaagagac gaacagtcga aaccgttgaa
     3061 tggtcattac taatcttctc aataaagaat tgataatctg caatcgcaat tacttgtggc
     3121 aaatgggaga tagccaaaac ctgaccatgc tgaccaattt tatgaatttt ctgagcaata
     3181 gcttgagcaa cacgacctga aactcccgta tccacctcat caaagacaat gctagtcttg
     3241 ccttctttac gtgaaaaggc agacttaatg gctaacatga gacgagataa ttcccctcca
     3301 gaagcaacct taaccaaggg tttaaagtct tctccagggt tggttgaaat ataaaactca
     3361 accattttat ttccctcacg actgaatttt cccttactaa aacgaacctg aaactgggct
     3421 ttttccatat aaagatcttg cagttcttgt ttaatctcag cttcgagttg ctgagccaaa
     3481 ttatgacgag cagaagcaag ttgacctgcc aaattgacaa gattgacttc caacttctta
     3541 agctctgctt ccatgtcctc agacgaaaga ttattgcctg tcaagagatt gtattcttcc
     3601 gtaatcttgg caaaataaag caaaacatca tcaacagtcc caccatactt acgagtaata
     3661 gtatgaagga ggtccaaacg attctcaacc tgcatcaggc gattgccatc aaaatcaagg
     3721 tcctcaatga tagcttccaa acgtttgcta atgtcttcta aaacatagta ggtctcagac
     3781 agagagcttg aaatttcacg gtattcagga tcatactctt cgacactttc catgtcattc
     3841 atagctgaac gaacattggc cagacttgaa aaatcttcat tgtccaacat actgtaggca
     3901 ttggtcagtg tatccgcaat atttttgtgg ttgaggagtt tatctcgctc ttgattgaga
     3961 gccaagtctt ctccagcctg caagtttgct gcctcaatct ctgccatttg aaattccaac
     4021 atttcgatac gtgccttgtg ttcctgttgg tttttcttga cttccagaac ctgcttgcgc
     4081 attttccgat aggcatcaaa actcgtttga taggtttctt tcaagtccca aaaagcggca
     4141 tcaccaaatt catccaacat ctggatatgc agttggggac gcattaactc ctcatggtca
     4201 tgctgaccat gaatatctac aagatgttgc ccaatagctc gcaaaacaga cagattaacc
     4261 atctgaccat ttacacggct gatactacga ccattttgca agatttcccg acggatgata
     4321 atttcatcac ctaattctaa accttgctca tcaaaaattt cctgtaaaag acgactattc
     4381 tcaactgaga aaagcccctc aatctctgcc tttggtgcac catgacgaat aacatctgtc
     4441 gtcgcacgag ctcccaacat catattcatg gcatcaatga taatcgactt ccctgcaccc
     4501 gtttcaccag tcaggacagt catccccttt tcaaaattga gggaaatagc ctcaataatg
     4561 gcaaagtttt ttatcgaaat ttcaagtaac atatagacct accaattttt tacttgttca
     4621 aagatttcct ctgctagact tccacttctg gcaatgacta aaatcgagct atcatcagtc
     4681 aaacagctaa aaatcttgtc tgcaaaagtc tcgattaact gagcttttac aaaagccgta
     4741 tttcctggaa taacttggag attgatcatc ttatccatca attcagccga ttcgatattg
     4801 tcttcagcca gttgcagact ttttacgatt gattttggca attcgtagac ataggtgttg
     4861 tctctcaaag gaattttgac aatacctaac tctttgatat ctcgggatac cgtcgcctga
     4921 gtggcagtga tacctgcttc tttcaaatgt tctacaattt cttcttgcgt gccgatttga
     4981 taatctgtca ccaatcttct aattttttca agtctctctt ttttattcat ttttaaattg
     5041 actatgcgcc ctctctactg cttctttaat ctcagcaaga atctgattgc ttgctgactt
     5101 ttcttttttc aaatacgcta aaaattcaat atttccatgt ccaccttgga tgggagaaaa
     5161 gtccaagcca aggactgaaa aacctacctc tactgccata gctgttacag attcaaggac
     5221 attctgatga accttagcat ctcgaataat tccatttttc ccaatctgct cacgtcctgc
     5281 ctcaaactga ggtttgacaa gtgctaccac ctgaccttga tcagccaaga cacggtgcaa
     5341 ggctggcaaa atcagactaa gggaaatgaa actcacatca atactggcaa agctcggctc
     5401 ctgctcgaaa tcagtctttt cagcatagcg gaaattgaac tgctccatgc tgacaactcg
     5461 tgggtcttgg cgtaatttcc aagccaactg attggtacca acatcgactg caaagaccaa
     5521 cttggcacta ttctgtagca tgacatcggt aaaacctcca gtagaggccc cgatatcaat
     5581 cgtagtcgcg ccatccaccg acaaatcaaa gacctgcaag gccttttcca gtttcaaacc
     5641 accacggctg acatacttga gtttctcccc cttgagtttt aattcggtgt catctggaat
     5701 tttctctcct ggcttgtcaa accgttctcc attaaggact gctacgacta ggccagccat
     5761 cacacctcgc ttggcctgct ctctcgtttc aaacaacccc tgtttataag ctagtacatc
     5821 cactctttcc ttagccattg attctcaaac tttctactac acttacaatc gattctgttt
     5881 caaagggaag ctgctgggca atttcttcta atttttcatt agcttgatcc agggtttggt
     5941 tacaaaaggc aatggactct tccaagccca acagggcagg ataggttgat ttttctgcct
     6001 gcagatcctt ttgaggtgtc ttgccgattt cctcaaaact agctgtcaca tccagtacat
     6061 catctctgac ttgaaaagca agtccaatca attcacccac agttttcagc ttcacctgca
     6121 tttcaggtga caattcagct ataatagctg ccgcttggaa gggataggct agtaacttcc
     6181 cagtcttatt ggcatgaata gtctgaagtt cttccaaaga caagtgctgg tgttcgccct
     6241 ccatatccaa aacttgccct gccaccatac ccagactacc tgaagcaagg gataagttgg
     6301 caatcaagtc caccttaatc tgacttggca aatctgcctg cgcaatcaag gcatatgagt
     6361 ctaagaataa ggcatctcca gccaaaatgg ccatagcttc accgaatttc ttgtgattgg
     6421 ttaaccgccc tcttcgataa tcgtcatcat ccatagcagg aaggtcatcg tgaatcaagc
     6481 tccctgtatg aatcatctct aaggcagtag ctacctgcgc gtgagcaggt ttgatggtaa
     6541 cctgcaaggc ttccagaact tctaacaaga gaaaaggccg aatacgcttg ccaccagcat
     6601 gaatagaata gagaacagac tcccgtaaac tagaggcaaa ctgctggtct ccataaaaat
     6661 cttccaaagc cgactcgaca agagctaatt tttcttgctt tttcattcaa aatcactttc
     6721 tgttccgtct tcttgcatga ccttgaccaa ggtcttttca gccttgtcca gcgtagcttg
     6781 gagctctttt gacaagacca tgcccttttg aaaggcagta atcgcatctt ccagagcaat
     6841 ttcaccattt tccaaacttt ggacaatggt ttccagttct gctagatttt cctcaaattt
     6901 cttttgtttt gacatcttta acctctaatt ctacttgacc atctcgcatc aaaagcgtta
     6961 cttggtcttt tttcttcaaa ctctcaaccg aatctacaac ggactcttct tttttgacaa
     7021 tagcataacc acgcgccacg attcggctag tatccaacat gagcaaagct tccgaaagtc
     7081 gcttggcctc agcaaccttg gcgtcataaa ctaacgccat ttggctacct aagagcttgt
     7141 ccaactgtcc taaacggtct tgatagcgtt ggattttggt aacaggtgat aattgtacta
     7201 attgatgagt tcttgcttga actaattgtt tgttatcaga aatccgagtt cgcaaacttt
     7261 gtttcaaacg cagttgcagt tggtccaagc gttgcaaata accgtcatac aagcgctcag
     7321 gttgtctaaa gataacagac tgactgcatt ttttcaaagc ctcttgtttc ttagatagaa
     7381 catttcggac tgccgttacc atccgttttt cctgattttg caaatgagct aatacatcca
     7441 acttggtcac aggtgttgcc agttcagccg ccgctgttgg cgttgcagcg cgtcgatctg
     7501 ccacaaaatc tgccaaggtc acatccgtct catgccccac actagagata actggcaaac
     7561 gagattcaaa aatagctcgt accacaattt cttcgttaaa ggcccagaga tcctcaatag
     7621 aaccacctcc acgaccaata atgagcaaat ccaaatcgtc ccgttgatta gcacgcgcaa
     7681 tatttctagc aatttcctcc gcagcccctt caccttgaac cttggtcgga taaagaagga
     7741 tgtcaacacc tgggaatcgc ctgctgacgg tcgtgataat atctcgaata acggctccac
     7801 tacggctggt tactacacca attctcttag aaaattgggg cagagcttgc ttgaagcgtt
     7861 cttgaaacag gccttcttct gtcaattttt tcttaagttg ttcaaactga atcgcaagcg
     7921 ccccaacccc atcaggctca gctttttcaa tgatgatgga gtagttacca cttggttcat
     7981 agacctgtac acgcccaatc acattgatct tcattccttc ttccaggtca aaccctaatt
     8041 tctgataaat cccagaccag atggtcgctt gaataactgc atggtcatcc tttagggaga
     8101 aatattggtg agtaggtcgt ttacgaaagt tggaaacttg accagttaaa tagacccgtt
     8161 ccaagtatgg gtctttatcg aatttcattt tcagatactt ggtcaaagtt gttaccgata
     8221 aatacttttc catctccacc tactattcat ttacttgctc tttcatgggt attattatac
     8281 caaaaatatg cctaaaaatc tccatttatg taccattatg agggaaaaat agaaaaagga
     8341 ggcaaggcct ccacatgtga ttatttgctg tttcgagctt cttccaaaat ctttgcaatc
     8401 ttggtcgtca acaggtcgat agccacggta ttgctaaccc cttcaggaat gacgatatca
     8461 gcataacgct tagttgactc gataaactgg tggtacattg gtttgaccac acctaagtac
     8521 tggttaataa cgctatcaag gctacggcca cgctcctcca tatcacgctt gatacgacga
     8581 ataatgcgca catcgtcatc cgtatccaca aaaatcttga tatccatcaa atcgcgcaga
     8641 cgcttgtcct ccaagaccaa aataccctca acgataaaga catcttgagg ttcctgacga
     8701 taggtcttgc tactccgtgt atgctctgta tagtcgtagg tcgggatgtc caccggacgc
     8761 cctgccaaca attccttaat ctgctcgatc atcaagtctg tatcaaaggc aaaaggatgg
     8821 tcatagttgg ttttgacacg ctcttcaaag gtcaaatgag actgatcctt gtagtatgaa
     8881 tcatgctcaa tcatggaaat cttttcatca ggaaaatgcg ataaaatggc tcttgaaaca
     8941 ctggttttac caccaccaga accacctgtc actccaataa tgattggtct attttgcatg
     9001 ctatcctccg ttattttatc cgtcgtctat tctatcacat tttttacaaa atttatagtc
     9061 tcatcagcaa aaaacaccag agtcttccct ctgatgttcg acagttttaa cgaaagccct
     9121 gtagggtttg agcaagcatc tgcaagaaag ccagtctccc tctttcctgg ctaggagctt
     9181 aatccataaa ccaaaatcaa tgacagactt aggtgcctgg taatgatgcg tcatgaggta
     9241 aagtgacaac ccttgactca aattggccat gaccgattgg tatttttcat ttctgtctaa
     9301 attttgtata gcaagacgaa acaggtgact caactcacta ttttcattta tttctttttg
     9361 ttgaagaagt tgctccaccc tttctcgaaa ctcatttcgc atcatggaat tatcacacca
     9421 aatcctgggc ctaagttccc atcagaacct gtaatggact ctaaatattt atctgaaa
//
LOCUS       DAVHIU010000070         9445 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVHIU010000070 DAVHIU010000000
VERSION     DAVHIU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.072"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            12..230
                     /locus_tag="VVN06_001430"
     CDS             12..230
                     /locus_tag="VVN06_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /protein_id="HEU5562950.1"
                     /translation="MSQFAVMDNEMLARIEGGDVSDIYRGYANQVSPFGSYPPILKNS
                     GPFPVSGYCPRGYHDRGYIGAGFHLCGI"
     gene            262..951
                     /locus_tag="VVN06_001431"
     CDS             262..951
                     /locus_tag="VVN06_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562951.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            993..1226
                     /locus_tag="VVN06_001432"
     CDS             993..1226
                     /locus_tag="VVN06_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562952.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1377..1988
                     /locus_tag="VVN06_001433"
     CDS             1377..1988
                     /locus_tag="VVN06_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5562953.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2149..2943
                     /locus_tag="VVN06_001434"
     CDS             2149..2943
                     /locus_tag="VVN06_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU5562954.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            2940..3575
                     /gene="trmB"
                     /locus_tag="VVN06_001435"
     CDS             2940..3575
                     /gene="trmB"
                     /locus_tag="VVN06_001435"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU5562955.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3701..4180
                     /gene="rimP"
                     /locus_tag="VVN06_001436"
     CDS             3701..4180
                     /gene="rimP"
                     /locus_tag="VVN06_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU5562956.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4224..5360
                     /gene="nusA"
                     /locus_tag="VVN06_001437"
     CDS             4224..5360
                     /gene="nusA"
                     /locus_tag="VVN06_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU5562957.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5382..5675
                     /locus_tag="VVN06_001438"
     CDS             5382..5675
                     /locus_tag="VVN06_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU5562958.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5668..5967
                     /locus_tag="VVN06_001439"
     CDS             5668..5967
                     /locus_tag="VVN06_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU5562959.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            5984..8776
                     /gene="infB"
                     /locus_tag="VVN06_001440"
     CDS             5984..8776
                     /gene="infB"
                     /locus_tag="VVN06_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5562960.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9027..9377
                     /gene="rbfA"
                     /locus_tag="VVN06_001441"
     CDS             9027..9377
                     /gene="rbfA"
                     /locus_tag="VVN06_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5562961.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2888 a         1776 c         2192 g         2589 t
ORIGIN      
        1 atacaaaaat gatgtcacaa tttgcagtta tggataatga aatgcttgct cgtatagagg
       61 gtggggatgt atctgatatt tatagagggt acgctaatca ggtaagtcca ttcggttctt
      121 accctcctat attaaaaaat tcaggtcctt ttccagtaag tggttactgc ccgcgcggtt
      181 atcatgaccg tggttatata ggagcaggtt ttcatttatg tggaatatag gctgaaatac
      241 aagaaatgag gaagaatttc tatgaaaaag tatcaacttc tattcaaaat aagtgcagtt
      301 ttctcttact tatttttcgt atttggtctt tctcagatga cgcttattat tcaaaattat
      361 tggcaatttt cttcccagat tggcaatttc gtctggattc aaaatttctt gagtttgcta
      421 tttagcggag tcatgatttg gattctggtt aagacaggtc atggttatct ctttcacatt
      481 ccaagaaaaa aatggctttg gtattcgatt ttgacagtat tagtggtagt gctccagatc
      541 tcttttaacg ttcagacagc taaacatgtt cagtcaactg ctgaaggttg ggctgtattg
      601 ataggttata gtgggaccaa ctttgctgag ctaggtatct atgtaaccct gtttttcctg
      661 actccactaa tggaagagtt gatttatagg ggattacttc aacacgcctt ctttaagcat
      721 tcgcgatttg gccttgattt gcttcttcct tccattttgt ttgctcttcc tcattttttg
      781 agtctgccta gtctgttaga tatcttcgtc tttgcaacat ttggaatcat ctttgccggt
      841 ttgacccgct ataccaagag tatttatcca tcctatgcgg tgcatgtgat caataatatt
      901 gtagcgacct tcccgttttt gctcactttt ctacataggg tcttggggta aaaaaacaaa
      961 aggcttgctt ttcagccata gaggaggtca tcatgtataa acacttattt ttcctagatt
     1021 ccaaaacttt agatcggttg acaccctata ttctagtctt ggcttctgac accattgctt
     1081 ttaatgtttt tgtgctaacc tttgtatctg cggtggtttt taatttccta aattccatgc
     1141 tagctttaat ggctatattc ataggggctg gctatgtggt cggattttgg ttactaatac
     1201 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gctgtacttg agtacggcaa
     1261 ggcgacgttg acgtggtttg aatttgattt tcgaagagta taaaatggtt tgttttggaa
     1321 aggctagaac taaagaatga cgtgtaggaa gtctgtttgt tgaggaggat aattttatgg
     1381 agttttttga taaatttcat gccttgtgtt ttggattttt agtactaata attgtcatta
     1441 cagttcctta tacgattaac catggggatt tttttcaaaa tgaatctgca ttgattattg
     1501 taagtcttct tgtaacctcg ctgagtgttg cttatgctag aaagtttgaa atgatttctt
     1561 ttgggatgtt aagcaagaaa caacttttgc ttttcattgc aatctttctt ctaagtgtac
     1621 ttgagacgct ggtttatatt catttcttcg ctgtttcttc tggctcaggg gtccaacact
     1681 tggcggaagt cagcagagga atttccctgt ctttgatttt gactacctca gtttttggcc
     1741 ccatccagga ggaactcatt ttcagaggac ttcttcaagg tgcggttttt gacaattctt
     1801 ggttaggact tgtgctaact tcctctctct tttctttcat gcatggacct tctaatgtcc
     1861 cttcgtttat tttttatcta cttgggggct tgttgctggg ccttgcttat aaaaagagcc
     1921 aaaacttatg ggtttctact ctagtccaca tgttttacaa cagttggcca ctcctatatt
     1981 atttataaaa attatgaaaa ggtaagcaga agacggtgct tacctttttc aagctataat
     2041 gatacccaag ccaatccaga ggcttttctt tgagaatcgg gttagagtgg ttgacgaata
     2101 ggccaaaaac tagtagaata gtaaggaaac tttatacgga ggaaagaaat ggatttgggt
     2161 gataatgagc taacactgac tcccatacct gggaaaagtg gcaaggctta tatgggtagc
     2221 tatcctgatg ggaagcgcat ctttgtaaaa atgaacacct ctccaatcct acctggtcta
     2281 gctagagaac aaattgctcc acaattatta tggagtcgtc gtttggcaga tgggcgtgat
     2341 atgtgtgctc aagaatggtt gacaggcaag atattgaccc cctatgatat gaatcgtaag
     2401 caaatcgtca atattttaac ccgtcttcat cgctcacgtc cgttgatgac acaattgagt
     2461 cgtttgggct atgccatgga aacacctgta gatttactac agtcttggca agaaacggct
     2521 ccagatgctt tgcgtaaaaa tcattttatc agtgaagtga tggctgattt acgtcagact
     2581 attccaggat ttagagagga ccatgcgacc attgtccatg gagatgtacg acatagtaat
     2641 tggattgaga cagatagtgg cttgatttat ttagtagatt gggattcggt tcgcttgacc
     2701 gatcgcatgt ttgatgtggc ccatatgctc tgccattata tttcagaaca tcagtggaag
     2761 gaatggttga cctactacgg ttacaagtac aatcaaacgg tattaagtaa attgtattgg
     2821 tatggtcaat tgtcttattt gagtcagatt tccaagtatt atatgaacca agatttagaa
     2881 aatgtcaatc gggagattca tggtttgcgt catttccgag acaagtatgg aaagagaaga
     2941 tgagagttag aaatcgtaaa ggggcaacag aattactaga ggcaaatccc cagtatgtgg
     3001 tcctcaatcc cttggaagcc aaggcaaaat ggcgggactt gtttggcaat gataatccca
     3061 ttcatgtgga agttggaagt ggaaagggtg cctttgtttc aggtatggcc aagcaaaacc
     3121 ctgacatcaa ctatatcggg attgatattc aaaagtctgt tttgagctac gctttggaca
     3181 aagtgcttga agctggagtg tctaacatca agctcttgtg ggtagatggt tctgacttga
     3241 ctgactactt tgaagacggt gagattgatc gcttgtatct gaacttttca gatccatggc
     3301 cgaaaaaacg ccatgaaaag cgtcgtttga cctacaagac cttcttggat accttcaaac
     3361 gtatcttgcc tgaaaatgga gaaattcatt tcaagacgga taaccgtggc ttgtttgagt
     3421 acagtttagt gagcttttct caatatggca tgaaactcaa tggtgtctgg ttagatttgc
     3481 atgccagtga ttttgaaggc aatgtcatga cagaatacga gcaaaaattc tcaaacaagg
     3541 ggcaagttat ctaccgagtt gaggcagaat tttaagagat aacctaaaat taggctgtac
     3601 aagtgctttt gctttacata agttggcaaa cgtgctatac tgatagtaag aatatgaaaa
     3661 gtgaggcggg gaaatatctt cgcctcttgc ttatgaggag gtggacgcaa tcgcaacaat
     3721 cgtagaatta gtcagagaag ttgtagaacc tgtcatagaa gctccttttg aactcgtgga
     3781 tatcgagtat ggaaagattg gcagtgacat gattctcagt atttttgtag ataaacccga
     3841 aggaattacc ttgaacgaca cggcagactt gacagaaatg atcagtcctg tcctagacac
     3901 catcaagcca gatcccttcc cagaacaata tttcctagaa attaccagtc caggtttgga
     3961 acgtcctttg aaaaccaagg atgccgtcgc tggagcggtt ggaaaataca tccatgtcgg
     4021 gctctaccaa gccatcgata agcaaaaggt ctttgaagga accttgttgg ccttcgaaga
     4081 ggacgagttg actatggaat atatggacaa gacgcgtaag aaaaccgtcc aaattccata
     4141 cagtttagta tcaaaagcac gtttagcagt taaattatag aaaaagaaag gatagctttt
     4201 gaggattcaa aagtgaagaa aacatgagta aagaaatgct agaggccttc cgcattttgg
     4261 aagaagacaa gggaatcaaa aaagaagata tcatcgacgc agtagtagag tcgcttcgtt
     4321 ccgcttatcg cagacgctat ggtcagtcag acagcgtagc tattgacttc aacgaaaaaa
     4381 caggtgactt tacagtttat actgtccgtg aagttgttga tgaagtattt gatagccgtt
     4441 tggaaatcag cttgaaagat gctcttgcca ttaattcagc ttatgaactt ggagacaaaa
     4501 tcaagtttga agaagcacca gctgagtttg gtcgtgtagc agcccaatct gccaaacaaa
     4561 ccatcatgga aaaaatgcgc aagcaaacac gtgccatcac ttacaatact tacaaagaac
     4621 atgagcaaga aatcatgtct ggtacagtag aacgctttga caatcgcttt atctatgtca
     4681 accttggtag catcgaagcc caattgtcaa aacaagacca aattcctgga gaagtttttg
     4741 cttctcatga tcgtatcgaa gtttatgttt acaaggttga agacaaccct cgtggtgtga
     4801 acgtctttgt tagccgtagc catccagaaa tgatcaaacg tttgatggag caagaaattc
     4861 cagaagttta tgatggaact gttgaaatca tgagcgtggc tcgtgaagca ggtgaccgta
     4921 cgaaggttgc tgttcgtagc cacaatccaa acgtggatgc tatcggtaca atcgttggac
     4981 gtggtggtgc taatatcaag aagattacta gcaaattcca cccagctcgt tacgatgcta
     5041 aaaatgaccg catggtacca atcgaagaaa atatcgatgt tatcgagtgg gtagcagatc
     5101 cagctgaatt tatctacaat gccatcgctc ctgctgaggt tgaccaagtt atctttgatg
     5161 aaaacgacag caaacgtgcc ttggtggttg ttccagataa caagctttct cttgccattg
     5221 gtcgtcgtgg acaaaacgtg cgcttggcgg ctcacttgac tggttaccgt atcgatatca
     5281 agtctgctag cgaatttgaa gccatggaag acgctgcttc agtagagttg gaagtagaaa
     5341 acgatactgt agaagaataa aagctgctag aggagggaaa gatgaaaaca agaaaaatcc
     5401 ctttgcgcaa gtctgttgtg tctaacgaag tgattgataa gcgtgatttg ctccgcattg
     5461 tcaagaacaa ggaaggacaa gtctttattg atcctacggg caaggccaat ggccgcggcg
     5521 cttatatcaa actagacaat gcagaagccc tagaggcgaa aaagaagaag gtctttaacc
     5581 gcagctttag catggaagtg gaagaaagct tttatgacga gttgatcgct tatgtggatc
     5641 acaaagtgaa aagaagagag ttgggacttg aataagcaaa agataagtaa tctcttgggg
     5701 cttgctcagc gagcagggcg catcatatcg ggtgaagaat tggtggtcaa ggccattcaa
     5761 gacggcaagg ccaagttggt ctttctagct catgatgctg gacccaatct gaccaagaag
     5821 attcaagata aaagtcatta ttatcaagta gaaattgtaa ccgtgttttc aacactggaa
     5881 ttaagcatag cagtcgggaa atcgagaaag gttttggctg taacagatgc tggatttaca
     5941 aagaaaatga ggtctcttat ggaatagaag aggaggacat gatttgtcta agaaaagatt
     6001 gtacgaaatc gcaaaagaac ttggaaaaga aagtaaagaa gttgtagcgc gtgcaaaaga
     6061 gttgggcttg gatgtgaaaa gccactcatc aagtgtggaa gaagctgtcg ctgcaaaaat
     6121 tgctgccagc tttaagcctg cagctgctcc gaaagtagaa gcaaaacctg cagcaccaaa
     6181 agtaagtgca gaaaagaaag ccgaaaaatc tgagccagct aaaccagctg tagctaagga
     6241 agaggcaaaa ccggctgagc cagttgctcc gaaaacagaa aaagtagcag cgaaaccgca
     6301 aagccgtaat ttcaaggctg agcgtgaagc ccgtgctaaa gagcaggcag aacgacgcaa
     6361 gcaaaataag ggcaataacc gtgaccaaca acaaaacgga aaccgtcaga aaaacgacgg
     6421 ccgtaatggt ggaaaacaag gtcaaagcaa ccgcgacaat cgtcgcttta atgaccaagc
     6481 taagaagcag caaggtcagc aaaaacgtag aaatgagcgc cgtcagcaag aggataaacg
     6541 ttcaaatcaa gcggctccac gtattgactt taaagcccgt gcagcagccc taaaagcaga
     6601 gcaaaatgca gagtacgctc gttcaagtga ggaacgcttc aagcagtatc aggctgctaa
     6661 agaagccttg gctcaagcta acaaacgcaa ggaaccagag gaaatctttg aagaagcggc
     6721 taagttagct gaacaagcac agcaagttca agcagtggtt gaagtcgtcc ctgagaaaaa
     6781 agaacctgca gtggatacac gtcgtaaaaa acaagctcga ccagacaaaa atcgtgacga
     6841 ttatgatcat gaagaagatg gtcctagaaa acaacaaaag aatcgaagta gtcaaaatca
     6901 agtgagaaat caaaagaata gtaactggaa taacaacaaa aagaacaaaa aaggcaataa
     6961 caagaacaac cgtaatcaga ctccaaaacc tgttacggag cgtaaattcc atgaattgcc
     7021 aacagaattt gaatatacag atggtatgac cgttgcggaa atcgcaaaac gtatcaaacg
     7081 tgaaccagct gaaatcgtta agaaactttt catgatgggt gtcatggcca cacaaaacca
     7141 atccttagat ggggaaacaa ttgaactcct catggtggat tacggtatcg aagccaaaca
     7201 aaaggttgaa gtggataatg ctgacatcga acgtttcttt gtcgaagatg gttatctcaa
     7261 tgaagatgaa ttggttgagc gtccaccagt tgttactatc atgggacacg ttgaccacgg
     7321 taaaacaacc cttttggata ctcttcgtaa ctcacgtgtt gcgacaggtg aagcaggtgg
     7381 tattactcag catatcggtg cctaccaaat cgtggaaaat ggtaagaaga ttaccttcct
     7441 tgatacacca ggacacgcgg cctttacatc aatgcgtgcg cgtggtgctt ctgttaccga
     7501 tattacgatc ttggtcgtag cggcagatga cggggttatg cctcagacta tcgaagccat
     7561 taaccactca aaagcggcca acgttccaat catcgtagcc attaacaaga ttgataaacc
     7621 aggtgctaac ccagaacgcg ttatcggtga attggcagag catggagtta tgtcaaccgc
     7681 ttggggtgga gattctgaat ttgttgaaat ctcagctaaa ttcaaccaaa atatcgaaga
     7741 attgttggaa acagtccttc ttgtagctga aatccaagaa ctcaaggcag acccaacagt
     7801 gcgtgcaatc ggtacggtta tcgaagcgcg cttggataaa ggaaaaggtg cggtcgcaac
     7861 ccttcttgta caacaaggta ccttgaatgt tcaagaccca atcgttgtcg gaaatacctt
     7921 cggtcgtgtc cgtgctatga ccaacgacct tggtcgtcgt gttaaagttg ctggaccatc
     7981 aacaccagtc tctatcacag gtttgaacga agcaccgatg gcgggtgacc actttgccgt
     8041 ttacgaggat gaaaaatctg cgcgtgcagc aggtgaagag cgtgccaaac gtgccctcat
     8101 gaaacaacgt caagctaccc aacgtgttag ccttgaaaac ctctttgata cccttaaagc
     8161 tggggaactc aaatctgtta atgttatcat caaggctgat gtacaaggtt ctgttgaagc
     8221 cctttctgcc tcacttcaaa agattgacgt ggaaggtgtc aaagtgacta tcgtccactc
     8281 agcggtcggt gctatcaacg aatcagacgt gacccttgcc gaagcttcaa atgcctttat
     8341 cgttggtttc aacgtacgcc ctacaccaca agctcgtcaa caagcagaag ctgacgatgt
     8401 ggaaatccgt cttcacagca ttatctacaa ggttatcgaa gagatggaag aagctatgaa
     8461 agggatgctt gatccagaat ttgaagaaaa agttattggt gaagcggtta tccgtgaaac
     8521 cttcaaggtg tctaaagtgg gaactatcgg tggatttatg gttatcaacg gtaaggttgc
     8581 ccgtgactct aaagtccgtg ttatccgtga tggtgtcgtt atctatgatg gtgaactcgc
     8641 aagcttgaaa cactataaag acgacgtgaa agaagtgaca aacggtcgtg aaggtggatt
     8701 gatgatcgac ggctacaatg atattaagat ggatgatgtg attgaggcgt atgtcatgga
     8761 agaaatcaag agataagatt ttttgctcct ttcttaggtg gtgagggacg caagcaaacc
     8821 gatggtttca ttgcttattt ttgagcctag ggtctcaaaa atcccctgtg atgggactga
     8881 taaatcagtt ccatcacttt caccacggcg aaagaagcag atgacttcaa attgaacttc
     8941 gtttcaattt aaactgaaaa tcaagaagtt taaaatagct aggtctgctg gcctagcttt
     9001 tggttcaaag tagagaaagg aatatcatgg caaatcattt ccgtacagat cgtgtgggca
     9061 tggaaatcaa gcgtgaagtc aatgagattt tgcaaaagaa agtccgtgat ccacgtgtcc
     9121 aaggtgtgac catcacagat gttcagatgc tgggtgactt gtctgttgcc aaggtttatt
     9181 acaccatttt gagtaacctt gcttcggata accaaaaagc ccaaatcggg cttgaaaaag
     9241 caactggtac catcaaacgt gaacttggtc gcaatttgaa attgtacaaa atcccagatt
     9301 tgaccttcgt caaagacgag tccatcgagt atggaaacaa gattgacgag atgctacgca
     9361 atctggataa gaactaaaga agaggggttg cccctctttt ttggtggagg aaaataggtt
     9421 gaatttgaaa tggaaaaata ttctt
//
LOCUS       DAVHIU010000071         9405 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVHIU010000071 DAVHIU010000000
VERSION     DAVHIU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.073"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..1121
                     /locus_tag="VVN06_001442"
                     /pseudo
     CDS             1..1121
                     /locus_tag="VVN06_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1249..1452)
                     /locus_tag="VVN06_001443"
     CDS             complement(1249..1452)
                     /locus_tag="VVN06_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5562962.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1445..2527)
                     /locus_tag="VVN06_001444"
     CDS             complement(1445..2527)
                     /locus_tag="VVN06_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5562963.1"
                     /translation="MSKLQQILIYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYYGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2653..3435)
                     /locus_tag="VVN06_001445"
     CDS             complement(2653..3435)
                     /locus_tag="VVN06_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU5562964.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3435..4778)
                     /locus_tag="VVN06_001446"
     CDS             complement(3435..4778)
                     /locus_tag="VVN06_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5562965.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4877..6193)
                     /locus_tag="VVN06_001447"
     CDS             complement(4877..6193)
                     /locus_tag="VVN06_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU5562966.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6386..7612
                     /locus_tag="VVN06_001448"
     CDS             6386..7612
                     /locus_tag="VVN06_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU5562967.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7811..9385
                     /locus_tag="VVN06_001449"
     CDS             7811..9385
                     /locus_tag="VVN06_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5562968.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LNEKQTGFVIRNKGDK"
BASE COUNT         2768 a         1812 c         2065 g         2760 t
ORIGIN      
        1 ctggactaaa gatagagttt ctcttgaata cggtctccca agtcgtacga tacttcttaa
       61 ctggctagca caatacagga aaaacgggta tactattgtt gagaaaacaa gagggagagt
      121 acctaaaatg ggacgtaagc caaaaaagag acctgaagag aggacagaat tagaacgtct
      181 tcaagcagaa aatgagtacc tgagagcgga gaatgccatc ctaaaaaagt taagagaact
      241 ccgattgaag gaggaaaaag agaaagacag aaattgttta agaattaatg actgagtttt
      301 cgttagatct tcttctaaaa gccattaaac tagctcgttc gacctactac tatcacttga
      361 aacagctaga caaaacagat aaggaccaag agcttaaagc tgaaattcaa tccattttta
      421 tcgaacacaa gggaaattat gcttatcgtc gaattcattt agaactaaga aatcgtggtt
      481 atctggtaaa tcataaaaga gttcaaggct tgatgaaagt actcaattta caagctaaaa
      541 tgcgacagaa acgaaaatat tcttctcata aaggagacgt tggtaagaaa gcagagaatc
      601 tcattcaagg ccaatttgaa ggctctaaaa caatggaaca gtgctacaca gatgtgactg
      661 aatttgccat tccagcaagt actcaaaagc tttacttatc accagtttta gatggcttta
      721 atagcgaaat tatcgcctat aatctttcaa cttcacccaa cttagaacaa gtacaaacaa
      781 tgttggaaca ggcattcaca gagaagcact acgagaatac gattctccat agtgaccaag
      841 gctggcaata tcaacacgat tcttatcatc ggttcctaga gagtaaggga attcaagcat
      901 ctatgtcacg caagggaaac agccaagaca acggtatgat ggaatctttc tttggcattt
      961 taaaatccga aatgttttat ggctatgaga aaacatttaa atcacttaac caattggaac
     1021 aagctattgt agactatatt gattactaca acaataaacg aattaaggta aaactaaaag
     1081 gacttagtcc tgtgcaatac agaactaaat ccttcggata aattatttgt ctaactgttt
     1141 gggggcagta cattttaaca cttgatacta tgcgttttat tgtgggaaga ttttcctcat
     1201 tttctagtga aattgaactt tttctaacat cttttgatag atgacttctt aatcaattat
     1261 catttccatt gttgtttctt ggggtttcat cccttgtctt tgataaaagc ggagagcacc
     1321 ctcgttatca ttccagacac ttagggttag attattgcag ccaatttctt tagcataata
     1381 gcagaaaaat gacaataaaa tagcccctag atattttgaa aggaaagcat aggggctata
     1441 tagtttaatc aaaataaagc aaatctttgc tggtgcttgt aaagaggtcg aagccatcct
     1501 tggtaacaac accgcagtct tcaatacgaa caccgacttt accagggata tagatacctg
     1561 gttcaacaga gaagcacatg ccttcttcga tgaccatgtc gtttccttcc atgatagatg
     1621 ggaattcatg gacatccata ccgataccat gcccgagacg gtggttgaag tactcaccat
     1681 aaccagcttt ttcgatgacc tcacgggcag cgcggtccac ttcatgagca gtcacacctg
     1741 gcttgataaa gtcaagagca gcttgttggg cttcaagagt caagttgtaa atatctttct
     1801 tgaattggtc tggtttgccg acagcgactg tacgagtcat atctgacgca tagccattga
     1861 ccagaacacc caggtcaaag aggagaagag catcattttc aaccttatta gctgctggaa
     1921 tgccgtgtgg attcgcagca ttatcaccag tcaagaccat ggtatcaaag ctcatttcat
     1981 aaccttcacg tttcatggca aagtcgattt gtgcgatgat atctgtctca gtcttatcaa
     2041 gagaaatatt gtcaaaacca acatgaacag ccttgtcagc ataaagacct gcaaccatca
     2101 ttttttgcac ttcatcagct gatttgatga ggcgcatgcg ttggatacga ggagtgaggt
     2161 tgtcaaactc agcagtctca aaaactgttt tcaaaccata gtatttggtc aagatgagat
     2221 tgtcaaactc aacagcgaca cgtttgaagt caagttgtgg aagagcatgt ttgatttttt
     2281 gccatggatt ttcagaatcg acatagccca ctactgggaa ggaaacggtg ctacttgcac
     2341 gttctacttc aagagctggg acaaagagga gaggttcctg atccgctagg acaaagagga
     2401 acatttggcg ttcatgggga tcactgtaaa aaccagtgag gtaattgatt gtgacggggt
     2461 cagatacgac agcgacgtct agtttttctg attcaagata tattaggatt tgttgtaatt
     2521 tagacatatg ctaccttctt tctacccctc tattttggca aaaaatgaga gaaaatgcaa
     2581 gggagaaggg taaaagaaaa gacaaaaaac tccgacaaga taacggagtt ctttgatatc
     2641 attttccttt gtttaagaaa agtcagcctt gcttttgacg tcactgtact cttcagcgat
     2701 ttcttggctg agaatgtcct cataggcagg gagttggatg tcctgaccat atttcttctt
     2761 gagggcagta gtaactaggc gataagccag attggcatta cgagagagga taggcccgtg
     2821 gaagtaggaa ccaaagacat tcttataatg aaccccttca ccgacctttt cttcgttgtt
     2881 tccatttcca tagacaacct gtcccagcgg tttttggtca tcagagagga aggtacgacc
     2941 ttggtgattt tcaaatccat agtaggtttc atcgaaatct tcattgtgaa tcttgatgtc
     3001 accgataaaa cggttattgg tctggttgag cgtgtagtgt cccatgaccc ctagcccttc
     3061 gatacgtttt cctgaagctt caacataata ttgacccaat agttggaaac caccgcagat
     3121 agccagaact acaccgtcgt tttggatgta gttgtcaatg ctctcttttt tagcaggtag
     3181 gtcgtctgca atgatacttt gttcaaagtc ttgaccacca ccgaaaaagg ctatgtcgta
     3241 gtgattttca tcaaagtcat catggagaga aacgatgtca acggtcacat gggctcccag
     3301 tttttcagcc acatacttga gcatgaggat gtttccattg tccccgtagg tattcatgag
     3361 atttccgtag aggtgggcaa tgttgagctg ataggggtaa ttgccatctt ttgaggaaag
     3421 tgaagtataa accattagtt catctccttt ctaacaatct gacgactagc cagcagttca
     3481 cgaaattcca gcatggcagt ataagttgcc agaatatagg catgcttgca gtcttgattc
     3541 tcaatggtct tgagaacttg ctccagatta ctcgtttcag tgattttctc agctggatag
     3601 ccagtcactc ggaggcgacg agcgatttca gaatgacgaa caccgccagc gttgatttca
     3661 ggaatgtcca tgtcagtgat ttgctcaaag tctgcatccc agatccagct agtgtcaatt
     3721 ccatctgcat agttggcatt aaggaggaca gataggctaa atggataagg tgctagtttg
     3781 atcatttcga tagcttgggt tgcaccgact ggatttttaa tcaagacaag ggtacattcc
     3841 ttgtcaccga tatgaaaggt ttcttggcgt ccaaagacag cacggctctt gtcaaatccc
     3901 tgtttgatga gttgcgaatc ggcacctagg aaacgggcga tggccacagc agctagggcg
     3961 ttatagatat tatagagccc gccgatttgg ataccgtatt cttggccgtc tatgacaaag
     4021 cgagagcgat tgttggtcaa ctcaaccagt tttgtcaaac gatagtcgag atcaggacgt
     4081 ttgcatccac aaccttcaca gatataggca cccaagtttg cataggtatt atgctcatat
     4141 ttgaggatgc cttggcagtc aggacagaga atcccttcgg tattgtagtg agccagttgg
     4201 gctggtcctt tttccaagtc aaaaccaaaa tactctatag ggtttggaat agttggcttg
     4261 tagaaaagtg gactgtctcc gttaaggaga acagtagcag ttggaacttt ccgaatggct
     4321 tccaatatca tgttataggt agtatagatt tcaccgaaac ggtccatctg gtcacggaag
     4381 atattagtaa tgacaaaaag actaggctgg atatagtcac agatacgaga tagactggct
     4441 tcgtcaattt cgaggacggc aatatttttc ccagtttttg aagatttggc tgttaggaag
     4501 gttgttgcaa tccctgtaat catgttggca ccgcttgggt tggttagaac ttgaccataa
     4561 acctctttta aaatgccgac agtgagggca gttgtcaggg tttttccatt tgttccagtg
     4621 acaacgacaa tctcgtagtt cttagctagg ttttgtaaaa tatctttatc aaattgaagg
     4681 gcgactttac ctggtagcgt acttccacgg ccaagacggc ttaaaatgaa gtgggaagaa
     4741 cgcccagcaa gaaggcccaa agtagttttt aagttcatgt ttctattata tcataaaaga
     4801 ggagaaagac agatttgaga ttccgcagaa gtaaaaacag cccaaagagg gctgttcgat
     4861 acgattaaat ctcaacttaa atcgcaaaca agtcattcaa gttctcagcc aaggttgggt
     4921 gagtgaagat ttgttttgtg aagtaagtgt aaggaatctt gttgtccata gcaacagtga
     4981 tgatgttgat gatttcttga gaaccttctg agaagatgct tgctccaaga atttcttttg
     5041 tttcagtatt gacaacagct ttgaaggcac cgcgaaggtc tccatttacg tgaccacgag
     5101 gcattgctgc aacggggatt tccttaacag cgtatggaag tttcaaatca gctgcttggc
     5161 tttcagtcaa accaacttgt gaaagtgcag gtgtgatgaa catagtattt ggcacattga
     5221 gacggtcttc aagtgtgtag ctgccatctc cagcaaggta gctgtaaaca acacggaagt
     5281 catcaagtga aatgtaagta aattgaaggc caccgttgac atctccaact gcaaagacac
     5341 caggaacgtt tgtttgacag tgtttgtcta ctttaatagc accacgttca gttagttcaa
     5401 tatctgtatt ttcaagttga agtggttcta catttggttt gcgtccagtt gcgtagagaa
     5461 gggcgtcgaa acggtaagtt tcgtcttcag ttacgacaag cacttggtca ccatcgtttt
     5521 tgatttcagt agtatggata ttttgaagca attcaatgcc atcttcttcc atgtattgtt
     5581 tagcaagagc tgcgatggaa ggttctgcac gaggtaggaa tgtatccaag gcatctagga
     5641 ctgtgacctt gcttccaagt ttgttgtaaa ggccggcaaa ttcaagaccg atatttccgc
     5701 caccaaggat tccaagtttt tcaggtaatt tgtccaagct ttggatacct gttgagtcaa
     5761 agatgttttt gcttgtagca agtccaggga ttggcaagac gtttgaaaca gcaccagtgt
     5821 tgatgacgat tgtttcagca gtcagttctt tcttttcatc accagcttgg atttcgatga
     5881 ctttatttga aaggaagtga gcttccgcat caaagatatc tacgcctgta ccagcaacag
     5941 tcgcatagtt tttaccgttg aggcgaccag tgatcgtgtt tttagtagca atgacttctt
     6001 caaaagacaa gtccttttca gcagcaacta gcaaggtttt agttgggata caaccaatgt
     6061 tgatacaagt tccaccgtac atagccttgc tgcgttcaac gagggcaact tttttgccag
     6121 ctgaagccaa tttacctgct agtgttttac cagctttacc aaatccaata acaattaaat
     6181 cgtatgttaa catttatctt cctcctattc gaatttcatt ctagcacaag aaaaaaactt
     6241 ttgcacaatt ctgctacgga aaagacagaa aaagataaaa acgctttcca gacaaaaaac
     6301 tagtaatttt ttgacaagct tgataaacta aaatcctatc tatttttata taagatagaa
     6361 taaatgttcg aaaataggag gttttatgaa atcgaatcgt tatattattg cctttgctgg
     6421 ggtcatttta cacttaatgc tgggttcgac ttatgcttgg agtgtttatc gtaaccctat
     6481 tattgaaaaa acgggatggg atcaggcttc tgttgccttc gcctttagtc tagcaatctt
     6541 ttgtttgggt ttatcggctg catttatggg gcgtttggta gaaaaatttg gtccgaaagt
     6601 catggggagt ctatctgctt ttctatacgc aggtggaaat atcttaacag gatttgcaat
     6661 agaccgtcag gaactgtggt tgttgtatct agcttatggc attttaggtg ggcttggttt
     6721 gggagcaggc tatattaccc ctgtgtcaac gattataaaa tggtttcctg ataaacgtgg
     6781 tctcgcaaca ggtttagcga ttatggggtt tggttttgct tctttattga ctagtcccat
     6841 agcgcaacac cttatcgcag gggtagggat tgtagaaact ttttatattt taggagcaag
     6901 ttactttatt atcatgctcc tagcttcaca attcattaag cgtccaaatg agcaagagct
     6961 tgcaatttta tcttcttcag ggaaagaaaa aacagcctct ttgacgcaag gaatggctgc
     7021 aaatcaggct ctaaaaagca atcggtttta tatgctttgg attattttct ttatcaacat
     7081 agcttgtggt ttaggcttaa tttcagcggc atcgccaatg gcacaggaga tggctggctt
     7141 gtctacaagt catgcagcag taatggtggg tgttttgggg attttcaatg gatttggtcg
     7201 cttgctctgg gcgagtttgt ctgactatat cggtcgccct ctaaccttta gtatattact
     7261 gcttgtcaat cttttctttt ctctctcact ttggctcttt acagattccg ttttatttgt
     7321 agttgctatg tctattttga tgacttgcta tggagctggt ttttctttga ttccagctta
     7381 tctcagtgat atttttggaa ccaaggaatt ggccgctctg catggttata ttttaacagc
     7441 ttgggcaatg gctggtttag cgggacctat tttattagca gagacttata aaatggctca
     7501 ttcgtacaca caaaccttgt tcgtttttct cattttatac agtatcgcct tggctttgtc
     7561 ttattatcta ggtcgttcaa tcaaaaaaga aagtcaaaaa ccgcttacat gattttctgt
     7621 agaaatgggg ctatcttttg ctctggtctt gtgttatact agataggttg caaagaaaac
     7681 agtacttttc ttttgtggaa aagaagcaac acgattttat atctagttat atgaaaatac
     7741 gtcataaaaa gaaaagtata aactaagccc tatagggtgg cttcgcacca cctttagaaa
     7801 gaagaataac gtgaaattta atgaattaaa cttgtctgct gatttgctag cagaaattga
     7861 aaaagctggt tttgtagaag ctagtcctat ccaagaacaa actattcctt tggcccttga
     7921 aggcaaggat gttatcggtc aagctcagac tggtacagga aaaactgcag cctttggctt
     7981 gcctaccctt gaaaaaatcc gtacagaaga agcgactatc caagctttgg tcatcgctcc
     8041 aactcgtgaa ctagctgtcc aaagtcaaga agaactcttc cgctttggtc gtagtaaggg
     8101 agtcaaagtc cgttcagtat atggcggatc aagcattgaa aaacaaatta aggctcttaa
     8161 atctggtgcc catatcgtgg tgggaactcc aggtcgcctc ttggacttga ttaaacgcaa
     8221 ggccttgaaa ttacaagaca ttgaaaccct tatccttgac gaagcggatg aaatgcttaa
     8281 catgggcttc cttgaagaca tcgaagccat tatttcccgt gtacctgaga accgtcaaac
     8341 tttgcttttc tcagcaacta tgccagatgc catcaaacgt atcggtgttc agtttatgaa
     8401 agcccctgaa catgtcaaga ttgcggctaa ggaattgaca acagaattgg ttgaccagta
     8461 ctatatccgt gttaaggaac aagaaaaatt tgacaccatg actcgtctca tggatgtggc
     8521 acaaccagaa ctcgctattg tatttggtcg taccaaacgc cgtgtggatg aattgactcg
     8581 tggtttgaaa attcgtggct tccgtgcaga aggaattcat ggcgacctag accaaaacaa
     8641 acgtcttcgt gtccttcgtg actttaaaaa tggcaatctt gatgttttgg ttgcgacaga
     8701 cgttgcagcg cgtggtttgg atatttcagg tgtgacccat gtctacaact acgatattcc
     8761 acaagatcct gagagttatg ttcaccgtat cggtcgtaca ggtcgtgctg gtaagtcagg
     8821 tcaatctatt acttttgttg ctccaaacga aatgggttac cttcaaatca ttgaaaactt
     8881 gactaagaaa cgcatgaaag gtctcaaacc tgcaagtgta gaagaatcct tccaatcaaa
     8941 aaaacaggta gctctcaaga aaatcgaacg tgattttgca gatgaaacca ttcgtgccaa
     9001 ctttgagaaa tttggtaagg atgctcgcaa attggctgct gagtttactc cagaagaatt
     9061 ggcaatgtat atcttgagtc tgacagtcca agacccagat agccttccag aagtggagat
     9121 tgcacgtgaa aaaccactac cgtttaaacc atcaggtaat ggtttcggtg gtaaagctaa
     9181 gggaggtcgt ggaggccgtc gtggggatga ccgtcgagag cgtgatcgcc gtggcaatgg
     9241 tcgccgtgat gagttcaaaa aaggaagtcg tggcaacgat cgttttgata aggaaaaacg
     9301 ttaccgtaag gataataaaa aaccacgcaa tactttaaac gaaaagcaaa caggctttgt
     9361 tattcgtaac aaaggtgata aataagaaaa agcggttcaa aatga
//
LOCUS       DAVHIU010000072         9400 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVHIU010000072 DAVHIU010000000
VERSION     DAVHIU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.074"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(59..1039)
                     /gene="dusB"
                     /locus_tag="VVN06_001450"
     CDS             complement(59..1039)
                     /gene="dusB"
                     /locus_tag="VVN06_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5562969.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKGPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1026..1898)
                     /gene="hslO"
                     /locus_tag="VVN06_001451"
     CDS             complement(1026..1898)
                     /gene="hslO"
                     /locus_tag="VVN06_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU5562970.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2063..3490
                     /locus_tag="VVN06_001452"
     CDS             2063..3490
                     /locus_tag="VVN06_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5562971.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3649..5157
                     /gene="glpK"
                     /locus_tag="VVN06_001453"
     CDS             3649..5157
                     /gene="glpK"
                     /locus_tag="VVN06_001453"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5562972.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5199..7025
                     /gene="glpO"
                     /locus_tag="VVN06_001454"
     CDS             5199..7025
                     /gene="glpO"
                     /locus_tag="VVN06_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU5562973.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKRTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALAQNDLAELKN"
     gene            7095..7799
                     /locus_tag="VVN06_001455"
     CDS             7095..7799
                     /locus_tag="VVN06_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU5562974.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8096..8299
                     /locus_tag="VVN06_001456"
     CDS             8096..8299
                     /locus_tag="VVN06_001456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562975.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8386..8973)
                     /locus_tag="VVN06_001457"
     CDS             complement(8386..8973)
                     /locus_tag="VVN06_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562976.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2692 a         1878 c         2131 g         2699 t
ORIGIN      
        1 tgattttgtt cgctttttat atttaatatt atacttttgt cctaggctct tttttgcatt
       61 atactttttc caattgcaag agagcttcaa tctctgctag ggtgctagct tgcgaaatgg
      121 ctccacggag tttggcagcg ccagatgttc cacggagata gtgaggagcg aggccgcgga
      181 attcacgaac tgcgacgttt tctcctttga ggttaatcaa tcgtttcaag tgttcgtagg
      241 cgatcttcat cttgtcttca aatgtcaaat caggtaggat ttctcctgtt tcaaagtaat
      301 ggttgatttg gttgaagagg taaggatttc ccatggcagc tcggccaatc atgactgcgt
      361 cagcaccaac ttcttcgatg cgttgcttgg cttcttggac agtacggata tcaccgttgg
      421 cgatgaatgg aatcttggtt agagcttggg caaccttgta aagggtctca aggtccgcgt
      481 ggccagtata catttgttca cgggtacggc catgcatggc gagggcagaa acacctgcag
      541 cctcagcagc gagggcattt tctactgcaa gagatgggtc cgcccagccg gtacgcattt
      601 tgacagtaag tgggatatca aggacagact ggaccttgtt gatgatagag tagatcttgt
      661 caggaccctt gagccacata gcaccagctt cgttcttcac gattttgttg acagggcagc
      721 ccatgttgat atcgacgata tcggtcttgg tgttttcttg gatgaattct gctgcgcgtg
      781 ctaggctgtc ttcatcgcta ccaaaaagtt ggatagagac agggttttcg ccctcatcga
      841 tatgaagcat gtgcagggtt ttttcgttgt tgtattggat tcccttgtca gagaccattt
      901 ccattacaac gagtccagct ccaagctctt ttgctatggt acgaaaggct gagttggtca
      961 cgccagccat aggcgctaaa acggtacgat tgggaatctc aatattgcca atcataaaag
     1021 gtgtattaag atttgtcacg aatgagttcc tccaggtcct tttcatcaaa gttgtaagta
     1081 gtttggcaga attgacaagt gatttctgca ccgtggtctt cctctttcat ttcctgtaag
     1141 tctgagcttg gaaggctggc aagagcgttc ataaagcgtt catggctaca gtcacattgg
     1201 aaacggattt cttcttcaga aagacgcttg taggcttcgt ccccgtagat agccttgagg
     1261 agggcttcga tatggtcgtc gctttcgaga agagtagaga tagctggcat ttcttggatg
     1321 cgtttttcaa agcgagcaat ctcttctttc ttggctcctg gcaagacttg aactaggaaa
     1381 ccacctgcaa ccttgacctt gtcttcctcg tccaaaagga cattgaggcc gaccgctgaa
     1441 ggcgtttgtt ggctttcagt aaggtaaaag gcaaggtctt caccgatttc tccagagatg
     1501 aggggagtta tagagttgta aggatttcca gtaccgtagt ctgtgataac gaggaattga
     1561 ccatttccaa caaaaggtcc gactaggact tcaccagtcg cagtcttttt gatgtcaaca
     1621 ccaggatttt gaacatagcc tttgacgttc cccttggtat cagcgacggt gataatagca
     1681 cctagagagc tagatcccaa caccttaact gtaagtttgg tatttccttt ttcattggct
     1741 gcgagaatct ggctagcgat aagagttcga ccaagcgcta cagttgagct agcttgggtt
     1801 tgatgttttt cttgagcagt gcggacggtt tcagtgctat caaggacaaa agcacgaaag
     1861 gctccgcttt ctgatatagt tttaataatt ttatccatag ctactatttt agcataaaaa
     1921 tgcccaaagg gggagccgtg tgtttactga ttttcaggat aatggaccag gaaatcagca
     1981 tgaaaataaa aagagaaaca gattatttta gcatttgtca gatttatgct atgcttaagg
     2041 tagaaaatga aaggggtaac aaatgtattt aggagatttg atggagaaag ccgagtgtgg
     2101 tcaattttca atcctttcct ttctattaca agagtctcag acgaccgtca aggctgtaat
     2161 ggaagaaaca ggattttcaa aagcaaccct aaccaaatat gtcaccctgc tcaatgacaa
     2221 ggctttggat agtggcttag agctgactat tcactcagaa gatgaaaatc tgcgtctgtc
     2281 tatcggtgca gctaccaagg ggagagatat tcggagcttg tttttggaga gtgctgttaa
     2341 ataccagatt ttggtttatc ttctctacca ccaacagttt ttagcccatc agctggctca
     2401 agaattggtg attagcgagg ctacgcttgg tcgtcacttg gctggtttaa atcagatttt
     2461 gtcagaattt gatttatcca tccaaaatgg acgttggcga ggtccagagc atcagattcg
     2521 ctatttctat ttctgtcttt tccgaaaggt ctggtcgagt caggaatggg aaggtcacat
     2581 gcagaaacca gagagaaaac aggagattgc caatttagag gaaatctgcg gtgcaagttt
     2641 gtctgcgggg cagaaattgg acttggttct ctgggctcac atcagtcaac aacgtcttcg
     2701 ggtcaatgct tgtcagtttc aagtcataga agagaaaatg cgagggtatt ttgacaatat
     2761 cttttatctt cgtttgctga gaaaggttcc gtcctttttt gctgggcaac atattccact
     2821 aggagttgag gatggtgaga tgatgatatt cttctctttt ctcctatctc atcgcattct
     2881 tcctcttcat actatggagt atattcttgg ttttggaggg cagttggcag atttactgac
     2941 gcaattgatt caagaaatga agaaggagga actattgggg gattatacag aggaccatgt
     3001 cacctatgaa ctcagtcagc tttgtgctca agtctatctc tataagggct atattttaca
     3061 ggatcgctac aagtaccagt tagagaatcg tcatccatat ttactgatgg aacatgattt
     3121 taaagagaca gcagaggaga tttttcatgc tctgcctgct tttcaacagg ggacagattt
     3181 agataagaag attctctggg aatggctcca gttaatcgaa tatatggctg aaaacggtgg
     3241 ccagcatatg cggattggtc tggatttgac atctggtttt cttgtctttt caaggatggc
     3301 agccattttg aaacggtatt tggaatacaa tcgttttatt accattgaag cttatgaccc
     3361 tagtcggcat tatgatttgc tggttaccaa taacccgatt cataagaagg aacagacacc
     3421 agtctattat ttaaaaaacg acttggatat ggaagatttg gcagggattc gtcagttatt
     3481 attcacttaa aaggcttggt taatccaggt cttttttgtg aaattcacac aatctcctca
     3541 cattttttta aaaattaaaa aaagttgata aacaagaaag cgctttattt tgtatactag
     3601 taagtgtaaa gaggaaacac ctcaagatct ttatcaggag gacagtatat gtcacaagaa
     3661 aaatacatca tggccattga ccagggaact acaagttctc gtgccatcat tttcaacaaa
     3721 aaaggggaaa aggttagctc gagtcaaaaa gagtttaccc agattttccc tcaggcaggt
     3781 tgggttgagc acaatgccaa tgaaatttgg aactctgttc agtcagttat tgcgggtgct
     3841 ttcatcgaaa gtggtgtcaa gccaaatcaa atcgaggcaa tcgggattac caaccaacgt
     3901 gaaacaacgg ttgtctggga taagaaaaca ggacttccta tctacaatgc tatcgtttgg
     3961 cagtcacgcc agacagcacc tttggctgag caactaaaaa gccaaggtta tgtggaaaaa
     4021 ttccatgaaa agactggttt gattattgat gcttacttct ctgctaccaa ggttcgttgg
     4081 attttggatc atgtagaagg tgctcaagag cgagcagaaa aaggggaatt gctctttggt
     4141 actatcgata cttggttggt ttggaaattg actgacggtg cggctcacgt gactgactac
     4201 tcaaatgcag ctcgtaccat gctttataac attaaagaac tcaaatggga tgatgagatt
     4261 ttggaaatcc ttaacattcc gaaggctata cttccagaag ttcgttctaa ctccgaaatc
     4321 tacggcaaga cagctccatt ccatttctac ggtggagagg tgccaatctc aggtatggct
     4381 ggggaccaac aagcagccct ctttggacag ttggcttttg agccaggtat ggttaagaat
     4441 acttatggaa caggctcttt catcatcatg aatactgggg aagagatgca gttgtctgaa
     4501 aacaacctct tgacaaccat tggttacgga atcaacggta aggtttatta tgccttggaa
     4561 ggttctatct tcatcgcagg aagtgctatt cagtggcttc gtgacggtct tcgcatggtt
     4621 gaaaattcac cagaatctga aaaatacgct cgtgattctc acaacaacga tgaagtttat
     4681 gtcgttccag cctttacagg tctaggcgct ccatactgga accaaaatgc tcgtggttcc
     4741 gtctttggtt tgactcgtgg aacaagcaaa gaagacttta tcaaggcgac tttgcaatct
     4801 attgcttatc aagtgcgtga tatcatcgac accatgcaag tggatactca gaccgccatt
     4861 caagtactga aggtggatgg tggtgcagcc atgaacaact tcctcatgca gttccaggcg
     4921 gatattttag gcattgacat tgcacgtgct aaaaacctgg aaacaacagc tctaggagcg
     4981 gccttcctag caggtttgtc agtagggtac tggaaagact tggacgagtt gaaactcttg
     5041 aacgagacag gagaactctt tgagccatct atgaacgaat ctcgcaagga acaactctac
     5101 aagggctgga agaaggctgt gaaagcaact caagtctttg cggaagtaga cgactaatac
     5161 tggcagaata aagcgattta tttagaaagt gtgtaaatat ggaattttca aagaaaacac
     5221 gtgaattgtc aattaaaaaa atgcaggaac gcaccctgga cctcttgatt atcggtggag
     5281 gaatcacagg agctggtgta gccttgcagg cggcagctag cggtcttgag actggtttga
     5341 ttgaaatgca agactttgca gaaggaacat ctagtcgttc aacaaaattg gttcacggag
     5401 gtcttcgcta cctcaaacaa ttcgacgtag aagtggtctc agatacggtt tctgaacgtg
     5461 cagtggttca acaaatcgct ccacacattc caaaaccaga tccaatgctc ttaccagttt
     5521 acgatgaaga tggagcaacc tttagcctct tccgtcttaa agtagccatg gacttgtacg
     5581 acctcttggc aggtgttagc aacacaccaa ctgcgaacaa ggttttgagc aaggatcaag
     5641 tcttggaacg ccagccaaac ttgaagaagg aaggcttggt aggaggtgga gtgtatcttg
     5701 acttccgtaa caacgatgcg cgtctcgtga ttgaaaacat caaacgtgcc aaccaagacg
     5761 gtgccctcat tgccaaccac gtgaaggcag aaggcttcct ctttgacgaa agtggcaaga
     5821 ttacaggtgt tgtagctcgt gatctcttga cagaccaagt atttgaaatc aaggcccgtc
     5881 tggttattaa tacaacaggt ccttggagtg ataaagtacg taatttgtct aataagagaa
     5941 cgcaattctc acaaatgcgc ccaactaagg gagttcactt ggtagtggat tcaagcaaaa
     6001 tcaaggtttc acagccagtt tactttgaca caggtttggg tgacggtcgt atggtctttg
     6061 ttctcccacg tgaaaacaag acttactttg gtacaactga tacagactac acaggtgatt
     6121 tggagcatcc aaaagtaact caagaagatg tagattatct acttggcatt gtcaacaacc
     6181 gcttcccaga atccaacatc accattgatg atatcgaaag cagctgggca ggtcttcgtc
     6241 cattgattgc agggaacagt gcctctgact ataatggtgg aaataacggt accatcagtg
     6301 atgaaagctt tgacaacttg attgcgactg ttgaatctta tctctccaaa gaaaaaacac
     6361 gtgaagatgt tgagtctgct gtcagcaagc ttgaaagtag cacatctgag aaacatttgg
     6421 atccatctgc agtttctcgt gggtctagct tggaccgtga tgacaatggt ctcttgactc
     6481 ttgctggtgg taaaatcaca gactaccgta agatggctga aggagctatg gagcgcgtgg
     6541 ttgacatcct caaagcagaa tttgaccgta gctttaaatt gatcaattct aaaacttacc
     6601 ctgtttcagg tggagaattg aacccagcaa atgtggattc agaaatcgaa gcctttgcgc
     6661 aacttggagt atcacgtggt ttggatagca aggaagctca ctatctggca aatctttacg
     6721 gttcaaatgc accgaaagtc tttgcacttg ctcacagctt ggaacaagcg ccaggactca
     6781 gcttggcaga tactttgtcc cttcactatg caatgcgcaa cgagttggct cttagcccag
     6841 ttgacttcct tcttcgtcgt accaaccata tgctctttat gcgtgatagc ttggatagca
     6901 tcgttgagcc agttttggat gaaatgggac gattctatga ctggacagaa gaagaaaaag
     6961 caacttaccg tgctgatgtc gaagcagctc tcgctcaaaa cgacttagca gaattaaaaa
     7021 attaagaaaa aataaaagag gtggagggca gcattccttg tcgcccgtcc cttcttttta
     7081 atggagacag aaagatgatg aatgaattat ttggagaatt tctagggact ttaatcctga
     7141 ttcttctagg aaatggtgtt gttgcaggtg tggttcttcc taaaaccaag agcaatagct
     7201 caggttggat tgtgattact atgggttggg ggattgcagt tgcggttgca gtctttgtat
     7261 ctggcaagct cagtccagct catttaaacc cagctgtgac catcggtgtg gccttaaaag
     7321 gtggtttgcc ttgggcttcc gttttgcctt atatcttagc ccagttcgca ggggccatgc
     7381 tgggtcagat tttggtttgg ttgcaattca aacctcacta tgaggcagaa gaaaatgcag
     7441 gcaatatcct ggcaaccttc agtactggac cagccatcaa ggatactgta tcaaacttga
     7501 ttagcgaaat ccttggaacc tttgttttgg tgttgacaat ctttgctttg ggtctttacg
     7561 attttcaggc aggtatcgga acctttgcag tgggaacttt gattgtcggt atcggtctat
     7621 cactaggtgg gacaacaggt tatgccttga acccagctcg tgaccttgga cctcgtatca
     7681 tgcacagcat cttgccaatt ccaaacaagg gagacggaga ctggtcttac gcttggattc
     7741 ctgttgtagg ccctgttatc ggagcagcct tggcagtgct tgtattctca cttttctagt
     7801 ttatactctt cgaaaatcaa attcaaacca cgtcagcgtc gccttaccgt actcaagtac
     7861 agcttgcggc tagcttccta gtttgctctt tgattttcat tgagtattag aaaacaatta
     7921 tgttgataga gcttgggcaa gagcccaatt tcagcaaaaa atgaagtaaa tcttctcata
     7981 ataaaacgca tcatatcaag cacgaaaatt ccacgaggtc aactacagtc agaaagctga
     8041 acaacaagcc aaaacgccca aaaaaggcgg caaaaagcaa gcacctgcaa gcaacgtgcc
     8101 gaaatggtca aatcctgatt atgtcaacga attagaccca aaaatcgttg atatgctagt
     8161 agaatttcac aagtcacaag gcactttgga aactcccgag gcgcaagcag aaatcgccca
     8221 aaaacgtgaa gaaatcgagc aaaggagagc tgagcttgag ggtaaaaaac aagagctttt
     8281 gaaccgcttg aacaaataga gtttcgcaag tattatgctt acaaattact tgagcaatta
     8341 actaaaatat aaaccctgcc tttatatcta ggcagggttt atattttaga aattcacgta
     8401 ggttgttacg gtttttacat acccagtata gtttgagttt ctatagtatt cagtgataaa
     8461 cttccatttt ctttgagcaa catggatata agtacttgtt atgtagtatg gatatgggct
     8521 ttgtgaatcc aagtaagact gataagcttg tataccaaaa tatgctccac caattattgc
     8581 accccatgga ccccccaata aagcacctat cctaccaatc atataactga ttccagcacc
     8641 agtcatgaag ttagcgaatg tgttagcttg tttattccca tgtattgtgt tgacgtaatt
     8701 ccaaacatta ggatcgtatg atctaaaaga tatatttagg tcgatttcat tcttttgata
     8761 agccatataa aatgccccat tgatatagac gccgtcagca cgtcgttcaa tagtgtctac
     8821 acttccatct ggattgacaa cctcaagaac ttcatcgctt aaaatattta cttgcgtatc
     8881 tccgaaccgc actgatgagc cattctcaaa ctgagcctca ccagatacaa ctttagagtt
     8941 tgccgataag ctatcatcag caaaaacaaa caagcgacgg ggaaatgcta gacatacaga
     9001 aaacagacat aactagcaaa cacatgcatt taaacatctt agacataacg gaaactcctt
     9061 tgtatttttg atttttttca acttttatta tacaataaaa ccaaataaaa agaaagcggt
     9121 aacaatatgc ttaatgcgaa aattttttat atatttttat gtttgatcgt tatcgaaact
     9181 acaggcttgt tgttgttgaa aagaggtctc gaaatgggtt atttagacac agaagctatt
     9241 atcctcgcag ttttttcatt tgctttttac aacctatgtt cattcgcttg ggtctgctct
     9301 acaataaaaa acaataaaaa ataaatagac gtattttcaa aaaaaaaaaa aatgcatatt
     9361 tatcttagca aaacgacgat ttaaatcgtc gtttttttgt
//
LOCUS       DAVHIU010000073         9342 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVHIU010000073 DAVHIU010000000
VERSION     DAVHIU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.075"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(1..192)
                     /locus_tag="VVN06_001458"
                     /pseudo
     CDS             complement(1..192)
                     /locus_tag="VVN06_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(238..471)
                     /locus_tag="VVN06_001459"
     CDS             complement(238..471)
                     /locus_tag="VVN06_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5562977.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(477..1469)
                     /gene="plsX"
                     /locus_tag="VVN06_001460"
     CDS             complement(477..1469)
                     /gene="plsX"
                     /locus_tag="VVN06_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5562978.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1466..2236)
                     /gene="recO"
                     /locus_tag="VVN06_001461"
     CDS             complement(1466..2236)
                     /gene="recO"
                     /locus_tag="VVN06_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5562979.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGTCLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2233..3402)
                     /locus_tag="VVN06_001462"
     CDS             complement(2233..3402)
                     /locus_tag="VVN06_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5562980.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGECYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3551..4561
                     /locus_tag="VVN06_001463"
     CDS             3551..4561
                     /locus_tag="VVN06_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU5562981.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4719..4799)
                     /locus_tag="VVN06_001464"
                     /pseudo
     CDS             complement(4719..4799)
                     /locus_tag="VVN06_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4834..5271)
                     /locus_tag="VVN06_001465"
     CDS             complement(4834..5271)
                     /locus_tag="VVN06_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5562982.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5356..7989)
                     /gene="polA"
                     /locus_tag="VVN06_001466"
     CDS             complement(5356..7989)
                     /gene="polA"
                     /locus_tag="VVN06_001466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5562983.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8245..8595
                     /locus_tag="VVN06_001467"
     CDS             8245..8595
                     /locus_tag="VVN06_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU5562984.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            8810..8926
                     /locus_tag="VVN06_001468"
     CDS             8810..8926
                     /locus_tag="VVN06_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU5562985.1"
                     /translation="MVGVFRQQTLYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            8919..9152
                     /locus_tag="VVN06_001469"
     CDS             8919..9152
                     /locus_tag="VVN06_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562986.1"
                     /translation="MFSQLHMREEQVLLAQDYASETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         2637 a         2318 c         1751 g         2636 t
ORIGIN      
        1 cgtttttact ttggcaaaga tattctcaac cttgcttatc tccttagata gcgcatgatt
       61 acaggcttta tcttcaactg ttagcggctt gagtctgctg gatttacgtg gagtttgtgc
      121 ttgaggatat atcttcatga gcccttgata accactgtca gccaagattt taccagcttg
      181 tccgatattt ctcatttcta aaaaccatct acttccgttg acttggaact ccgattgcta
      241 ttttccttga atgattttaa ccacatctcc tacgttttgg agttggtcaa tttcttcatc
      301 gctgatttcg atactaaatt catcttccag agtcaagata aactccatca agtcaacaga
      361 atccgcatcc aaatcgtctt tcagactcaa ggattctgtc acgacaaagt cctctccctg
      421 tcgctcttgg ataatggtca caatacggtc aaaaatttct ttttctgtca tctcttttat
      481 tctcctgaaa attcacgcgc agtctgggca accacgtctg tttctagcat ggtacggatc
      541 tgacgaatcg tactataaac agccttggca tcgcttgagc catgagtctt gacaacaggt
      601 gccttaacac caaacaagac cgctccacca acatctgaat aattgagctg ttttttcaaa
      661 cctctgaggc tgtccttgag aaggagggca cctagtttcg ctcgaagacc accacctgta
      721 atagctgtct tgagcaagcc catgattccc atagctgtcc cttcgatgga tttgagcaca
      781 gcgtttcccg tgaaaccatc tgccacaaca acatctgcaa cgccattcat caaatcacgc
      841 gcttccacgt ttccgataaa gttcaaactt tcatcagccg ccagtaattc ataagtttcc
      901 ttacgaagcg ggtcgccctt gctactctct gttccgttgt taagcaagcc aacgcgtggt
      961 tgcgcaatgc cacgaacatt cttggcatag aaagaaccta ggacagcgta ttgatggagg
     1021 tgctgggctg tattttctgc attagcaccg aggtcaagca tgtcaaaacc tttcccatct
     1081 acagtcggca atgttgacat aagtccagga cggtcgatat tcttgatacg acccacgatg
     1141 aagaatccag cagccaacaa agcacctgta ttcccagccg aaaggacagc gtctgcttca
     1201 ccatctttga cagccttggc tgccaatacc atactggcat ttttcttatt ccgaatagct
     1261 ctcgtaggtt catcatccga atcaatcttc tcatccgtat ggataatgct gacgcgctct
     1321 gtcgctgtca gatattgctt gattttagct tcatctccgt agagttgaac ctcgatatct
     1381 gaaaagtcag atagggcttg attgacaccc tcaacgatgg cctgaggtgc gtaatcgccc
     1441 cccatggcat ctactgcgat ttttttcatt tcttttcctc ttttagtaat tgtccccagt
     1501 ctgctaggga atcaataaat ttctttgatt ttaggtgaat cccaacgtac tcttcatata
     1561 gttgatccat aaattggcgt agctcttgct tgattccagg cttgagcgaa atggtctcca
     1621 aagtctcaaa atcaatagct tgaaattgat tgagcagata ggggatattg ggattgagat
     1681 ggcaacgtct ctcatcctca tgataatgct ctggacagag acaggttcca tatttgaaag
     1741 aaaagtcaaa agcctgacca acccgatggc agaagacaca ctcattaaaa ttgaggctga
     1801 ttccaaatcg agtcaaaatt tgaatttcaa aaatattggt caaaacctga taatccaagc
     1861 ctgcttccat caactccaaa gtcttttgca aaaaagcaaa caagggagca tcctgctgat
     1921 tgtcctgcaa actagcatct gcaagagctg ccacataggt cgcataggcc atgacaaaga
     1981 ggtcactatt aatcttggga aaagtcatga cctcatgata gtcttcgatg taactgagtc
     2041 cgtcatcatt gattcgcaag agaaatcgtg ccagcaccaa gggctgaata acaggcgcca
     2101 gcttagactg accagcgtgt ttgacaaaaa acatgcgttt gccaacctgc tctgtaaaaa
     2161 ttttgacgag cttgtcatcc tcacgaaaat tgcgattgta aagcaccaag ccttgactcg
     2221 tgatagactg aatcatgctt ctctcatgta ctcctcaagt cgtttcatgg cttctttgat
     2281 agtctccatg ctggctgcat aagataggcg gacgtagcat tccccgtaac gtccaaaggc
     2341 tgcaccaggg ataaaggcaa cggccttctt ctgagcaaaa tccttcagaa aagcaaagga
     2401 gtcttgattg tagcccgctg gaattttagc aaaaatatag aaggcaccgt ctggtttgat
     2461 aatctcaaaa ccaagagcag tcattttttc gatgatatag tcccgacgct ggatatattc
     2521 cttcttcatg ggctccgcat catttttacc agccgtcaag gcttctaccg cagcatgttg
     2581 cgccatggta tttgcggcag tgaccaagta ttggtgactc ttgattaact gggctgtgaa
     2641 ggccgcagga gcgaaaatca gccccaaacg ccaacctgtc atggcatgcg atttagacaa
     2701 accattgata ataatagcct ggtctctcaa catcgttcct agagacacat gggcttcgcc
     2761 tgtgtaggtc aattctgagt aaacctcatc acagacaaca aaaatttcgt acttgcgtaa
     2821 aacagctgcc aaggcctcta actgctctcg actgtaggta attcctgtcg gattggctgg
     2881 atagttgaga ataaccgcct tgagcttatc accctgctcc aaaatggcct tctccaacat
     2941 ctcaggagtc aagacaaaac cattttcagt cgtatcaatc tcaacaattt ctgccccaac
     3001 tagattgaca atcggttcat aacccggata agcaggagct ggcaagagca ccttgtctcc
     3061 ctcttccaaa atagccgtca aagtcgcaga taaagcctct gtcgctccga tagtgaccaa
     3121 gatttcattt tcaggagcat agtccagttg gtacttttcc ttaacaaaat cactggccgc
     3181 ctgacgtaga gtcagcagac cactcatccc tgtatagtag gattggttct gatcaatcgc
     3241 tcgcttggcc gcctccttga catggtctgg cgttgtaaaa tcaggttccc ccaaggtcaa
     3301 acgcaagacc ccaggaatct ccgaaataac ctggtcaaac tgacgaatca acgaaacttg
     3361 aattttatct aactgtttat taaagcgctt agttaagtcc atagatacct cctactttca
     3421 aaacgtatct ctattatact acaaaagccc cccgaccgca aaaatacatt agagaatagc
     3481 tttccacttt ctattttact tttcctgttt acaggtctat aatggtaaca gattctattt
     3541 ggaggttttt atgtcatttc tatcaaaaaa tggagcaggc atcttggtct gccttctcat
     3601 ttctatcgta tcttggtact taggaggatt cttccctgtg attggcgcgc ccgtttttgc
     3661 gatttttatg ggcatgctcc tacatccctt tctctcgtct tataaacaac tggatgttgg
     3721 attgaccttt agttccaaga aattgctcca gtatgccgtt atcttgcttg gttttggtct
     3781 caatatctct caagtcttcg cagttgggca atcttcactc cctgtcatcc tttccaccat
     3841 ttcaatagcc ttgattgttg cctacctctt ccagcgcttc tttgccctgg atacaaaact
     3901 ggctaccttg attggagtag gttcttctat ctgtgggggt tctgccattg cagcgacagc
     3961 gcctgttatc catgctaagg aaaaggaagt agcccaagcc atctccgtta tctttttctt
     4021 caatgtcttg gctgcgctca tctttccaac cctcggcacc tggcttcatc tatccaatga
     4081 cggcttcgcc ctctttgcag gaactgcggt caatgatact tcctctgtaa ccgccacagc
     4141 tagcgcttgg gacagtctct accagaccaa taccctcgaa tccgcaacca ttgtcaaact
     4201 cacgcgaacc ctagctatca tccccatcac tctctttctc tcctactggc aaagtcgcga
     4261 gcaaaaaaat aagcaaggct tacaactgaa aaaagtcttc ccacttttta tccttcactt
     4321 catccttgca tcgctcctaa ccacccttct gacctctctc ggtgtgtcta gtagtttctt
     4381 tacccctctc aaacaactct ctaaattcct cattatcatg gccatgagtg ccatcggtct
     4441 caaaactaat ctggtcgcta tggtcaaatc cagtggaaaa tccattgttc tcggagccgt
     4501 ctgctggatt gccatcatcc tcaccagcct tggtatgcag acccttatcg gcattttcta
     4561 atactcttcg aaaatctctt caaaccacgt caacgtcgcc ttgccgtata tatgttactg
     4621 acttcgtcag ttctatctgc aacctcaaaa cggtgttttg agctgacttc gtcagttcta
     4681 tctgcaacct caaaacggtg ttttgagctg acttcgtcag ttctatctac aacctcaaaa
     4741 cagtgttttg agcagcccgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
     4801 aacacaaaag gtagcccatc agctaccttt ttcttatgct tcctcaatca agcgagtatg
     4861 ttctctcttg atacagcgat tcatcacgat atcatcacat ccaccatcac gcaaaatctc
     4921 tttcgcttct aaactttcaa gtcctagctg tgcccaaaaa atcttggcat cagctttgag
     4981 aaaatcacgc gccacatcgg gcagaaattc actgcgacga taaacattga caatatctac
     5041 aggaaaagga atttcagcga ggctagcata agccttttca cccaagattt cgccacctgc
     5101 cgccttggga ttgactggga tgattttata gccccgagcc tgcatttcct ttgttactcg
     5161 attgctggtt gtttcttcac ggtcagacaa acccaccaca gcaagggttt tactcgttgc
     5221 gagatactga cgaatcacgc catcacttgg attgataaat tcttgactca tagaaatcct
     5281 cctttttcat cagtatagca cattttgaaa aggtttgcag aattatacta caaaaaagga
     5341 ggactagccc cctttttatt tagcctcgta ccaggttgcc ccttcattct catctgcgat
     5401 aagaggaaca ctgagttgaa tggcttcttc catggtttgt ttcaccaatt ttttcatctc
     5461 taccaattca gatttaggca cttcaaggac gatttcatcg tgcacttgta acagcatctt
     5521 agtctgataa ccacctgcaa ccaaggcttt atccagctga atcatggcaa tcttgagaat
     5581 atctgctgcc gaaccctgga taggtgagtt gatagcagtt cgctccgcaa aaccacgaat
     5641 attgaagttg cgcgaattga tatctggcaa ctcacggcga cgcttaaaga gggtctctac
     5701 atagccctta tcacgcgcct cccgcaccac ttcatccatg tagtttttaa tacctggaaa
     5761 acgttcaaag taggtatcaa tgtaggcttt ggcttcctta cgactaattc ccaaattatt
     5821 agacaagcca aagtctgaaa tcccataaac cactccaaag ttaactgcct tggcattgcg
     5881 acggtcgttt gcagtcacat catcaggacg ctcaatgcca aagacccgca tggctgtcga
     5941 agtatggata tctgccccct cttggaaggc cttaatcaag tgctcatcct tagaaatatg
     6001 cgccaaaacg cgcaattcaa tctgtgaata gtcagagctg agtagcacac tatcctccca
     6061 ctctggcaca aaagccttcc gaatcaagcg cccctgttcc aatcgggcag gaatattttg
     6121 caagtttgga tccacactag acaaacgccc ggtctgggtc aaatcctgca catagcgagt
     6181 atgaatcttt ccatcagcca aaatccagtc ctgcaagcca attacataag tagattgaat
     6241 cttagcaatt tgacggtaat ccaggatttt cttaacaatc ggagcaatag gagcgagacg
     6301 ctccaacaca tccactgctg tcgaataacc tgtcttggtt ttcttagtgt attctagagg
     6361 aagtcccaat ttctcaaaga gaagcacgcc caactgctta ggcgagttga cattaaactc
     6421 ctcaccagcc agctcgtaaa tctcttgagt cagtttttca atgacaagct cattttcagc
     6481 ctgcatctca agcaaggtct ctttcttgac cataatccca gcaatttcca tcttggcaag
     6541 gacaaaagcc agaggttgct ccatatcata aagaagctct aattgcccat tttcgctgag
     6601 tttttcaagt aaaataggct ctgtttctac caaaacagca agtttacaag ctaagtgttc
     6661 caagaatttc tcacgttcag gaatggcctt tttaacaccc ttaccgtaga aagtttcatc
     6721 atcaaccaag taagtctgac cataaagact agcgatggtc gcaatttcat tgtcctccac
     6781 agtcgaaagg aggtatttag ccaaacggat gtcaaaagca ggcgcctgca aatccacacc
     6841 aaaacgttgc aaaagaactt taaccttctt aaagtcataa actctcagag atgttttttc
     6901 taagaaatcc ttgaaaatcg ggtcttgcaa cagctcaagc ttgtctgtgg catagagctt
     6961 atccccacaa gaccaggcaa atccaaccaa attatccgta tggtaattct caccaaaaag
     7021 ctcaaagtgg aagatagact cttcactcag catatcttga ctgatttggt caacaatagt
     7081 aaaatccaaa ctctcagaca catcagctga cgacacattt aaagcctgct ttagctgttt
     7141 gaagcccatc tcatcgtaga atttcccaag attttcaaca tctggaccac tatagaccaa
     7201 gtcctctaaa ccaatcgcaa tcggtgcctt ggtatcaatg gtcgctagtg ttttagataa
     7261 aaaggcctgt tccttgtcat tgatgagatt ttccttcatc ttagaagtct tcattccatc
     7321 aatattttca taaatcccct caagcgaacc atgctccagc aagagcttaa tacccgtctt
     7381 ttcaccgact ttggtcaccc cagggatatt atccgactta tcacccatga gcgccttgag
     7441 atcgataaac tgagctggtg tgaggcccat ttcttccatg aggtaatctg gcgtaaaggc
     7501 ctcaaactca gccacacctt tcttggaaat ttcaaccacc gtatgctcat ccgtcagctg
     7561 aatcaaatcc ttgtccccac tgacaatggt aatatcaaaa ccatcctgct ctgctagctt
     7621 atccagcgtc ccaatgatgt catccgcctc atactgagcc agctcatagt gacgaatccc
     7681 catatgatcc agcaactcac gaatgaaagg aaattgctca cgaaactcat caggagtctt
     7741 ggcccgacca cccttatagt ccgcatacat ctctgtccgg aaggtcgtct ttcccgcatc
     7801 aaaagccacc aaaatatgac tcggctcaac ccgctccaac aaatgactca acatcaactg
     7861 aaaaccataa atcgcattgg tatgcaaacc agccgcattc ttaaaacggt ccaactgctg
     7921 atacagcgca aaaaacgccc gaaaagctac agaagaccca tcaatcaata ataatttttt
     7981 cttatccata cacccattat aaaggaaaga atcaaaaaat accattggga agagctagag
     8041 caagtatttt tcaaactttt tccgaataaa tagatagagc cagagaattt agtaaaccta
     8101 gatttaaaaa tgtgctataa tatagtatat tgaatctata atagtacacc ttgactgcta
     8161 aaatatttct ataaattaat ttgactttcc tgatagagtt attcacatct tatttcaact
     8221 cactatagaa ggaggaatag gaggattctc agacatccgg gcatcagccc aactaatgat
     8281 ttgattgcta agaaaatatt cagcaatcca gaaatcactt gtcaatttat tcgcgatatg
     8341 ctggacttgc cagcaaaaaa tgtgaccatt ttggagggaa gcgatattca cgtattactc
     8401 tccatgcctt actcggtgca ggatttttat accagtatag acgtcttggc ggagttggat
     8461 aacggtactc aagtaattat tgagattcaa gtccatcatc agaatttttt catcaatcac
     8521 ttgtgggctt acctgtgcag tcaggttaat caaaatcttg aaaaaattcg tcagcgagaa
     8581 ggtgatactc actagatcta caaacacatc gctcctgttt acgtcattgc tatcgtggat
     8641 agtaattatt tctcagatga cctggctttt catagcttta gtatgcgcga agacacaaca
     8701 ggtgaggtat tggcgattac caacaatgga caggaaaacc atctggttaa gatggcattc
     8761 ttggaattaa aaaatacaga gaaaccagca aagacaaggt tcgcaagcca tggttggagt
     8821 ttttcggcaa caaacccttt acccagcaac cgcaacgagc cattacccaa gcaaatcaac
     8881 tgctggacta caagagctgg tccgaggagg acaggaaaat gtttagtcaa ctacatatgc
     8941 gagaagaaca agtcttgtta gcacaggact atgcctcgga aactgctagg gctgaaggcc
     9001 ttgaacaagg actagagcgt gggaaagttg aaggaagggc agaaaggaaa ctttttgcct
     9061 tcctagacat agtacgccaa ggtcttctga cttctgaggt tgccagccag caattaggta
     9121 tgtcagtatc tgaatttgag gcactgttgt aaaatggctc cataatatcc ataggggatt
     9181 tacccactat ggatattatg gagcctattt tgtgtaaaaa aaaaagtccc atatgaccta
     9241 taatgaaaag cgacaaaaca actcattaga aagaatcata tggaacaatt acattttatc
     9301 acaaaattac tagacattaa agaccctaat gtccagattt ta
//
LOCUS       DAVHIU010000074         9314 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVHIU010000074 DAVHIU010000000
VERSION     DAVHIU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.076"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            479..1522
                     /locus_tag="VVN06_001470"
     CDS             479..1522
                     /locus_tag="VVN06_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU5562987.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1689..3776
                     /gene="topA"
                     /locus_tag="VVN06_001471"
     CDS             1689..3776
                     /gene="topA"
                     /locus_tag="VVN06_001471"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5562988.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEVASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3882..4241
                     /locus_tag="VVN06_001472"
     CDS             3882..4241
                     /locus_tag="VVN06_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU5562989.1"
                     /translation="MRLIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4334..4966
                     /locus_tag="VVN06_001473"
     CDS             4334..4966
                     /locus_tag="VVN06_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU5562990.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQLGVTQLHGTKVVF"
     gene            5010..5666
                     /locus_tag="VVN06_001474"
     CDS             5010..5666
                     /locus_tag="VVN06_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU5562991.1"
                     /translation="MFEIFKSYQFNQEKVHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5685..6317
                     /locus_tag="VVN06_001475"
     CDS             5685..6317
                     /locus_tag="VVN06_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5562992.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNICQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6345..6779
                     /locus_tag="VVN06_001476"
     CDS             6345..6779
                     /locus_tag="VVN06_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU5562993.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6791..7828
                     /gene="leuB"
                     /locus_tag="VVN06_001477"
     CDS             6791..7828
                     /gene="leuB"
                     /locus_tag="VVN06_001477"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5562994.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERIAGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            7825..8094
                     /locus_tag="VVN06_001478"
     CDS             7825..8094
                     /locus_tag="VVN06_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU5562995.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8098..8457
                     /gene="leuD"
                     /locus_tag="VVN06_001479"
     CDS             8098..8457
                     /gene="leuD"
                     /locus_tag="VVN06_001479"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5562996.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8619..9242
                     /locus_tag="VVN06_001480"
                     /pseudo
     CDS             8619..9242
                     /locus_tag="VVN06_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2883 a         1638 c         2065 g         2728 t
ORIGIN      
        1 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatt ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataag aaatggtcta aaatagatga tgatactttt
      241 cgaaaatctt tttatgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatattgt
      481 gacaaataat aaactgtatt cgttgataga atttagaaat aaaatatatg aagaattaga
      541 actttccaga agtgatttag cgattttact atgtgccatg cttatcgcct ctatcggatt
      601 aaatatggat tcgactcccg tgattattgg agccatgtta atctctcctt tgatgacacc
      661 tattctggga gtggggctct ctctagctat atttgatttt aaattgttaa gaaaatcttt
      721 taaaatatta gctattcaaa ttcttgccag tttaatagct tcaacacttt atttttatct
      781 ttctcccatt tcgtatgcta gttcggaaat tgttgctaga acctctccga ctatttggga
      841 tgttctcatt gcttttgtag gagggatagc aggtattatt ggtgctagga aaaaagagac
      901 caataatatt gttcctggtg ttgctattgc aaccgccttg atgcctcctc tttgtacagt
      961 aggttatgct attgcttctg ctaatctaaa atttatcata ggctcctctt acctattcct
     1021 catcaattgt agctttattg tcattgcgac ttatataggt gttaggttga tgatggttaa
     1081 gaaacattat tttaaagata atgaagaaga ctctaaaatg cgtaggattt tgcttctagt
     1141 tgctgttttg ctgatgattc cgagtttcat ctctgcaacg actttagtga gagaaacgtt
     1201 gaaaaaagag tcccttaaga aatttatatc agagcagttt caggggcata atattttgaa
     1261 aaaaacctat tctaaaaaga ctcataccct aaagctaacc atttcaggaa attatttgac
     1321 agaagaagaa ctcgatatga tttcaagtaa gagaggtgac tatggtttaa gtgatgtttc
     1381 tgttcaagtt tcacaattgt ctgattcaga acaacttagt aaggaagaac tggtggagta
     1441 tttcttccag tatatcaagg ataaggaagc aaaagaaaag gaaaaagcta ataagtttta
     1501 tacagagtct gaggagcaat aatttcttga gaatagctgg tttttctcgt gagtcttcta
     1561 tgtatatcaa aggaagactg aggttttaag tataaaactt ttcttctatt atagtagcta
     1621 agtcttgaca gggttcaaaa aatggtttac actttataaa gtttattact ttgaaaaggt
     1681 gtgatactgt ggctacggca acaaaaaaga aaaaatcaac agttaaaaaa aatctagtca
     1741 tcgtggagtc gcctgctaag gccaagacga ttgaaaaata tctaggcaga aactacaagg
     1801 ttttagccag tgtcgggcat atccgtgatt tgaagaaatc cagtatgtcc gtcgatattg
     1861 aaaataatta tgaaccgcaa tatattaata tccgaggaaa aggccctctt atcaatgact
     1921 tgaaaaaaga agctaaaaaa gctaataaag tttttctcgc gagtgacccg gaccgtgaag
     1981 gagaagcgat ttcttggcat ttggcccata ttctcaactt ggatgaaaat gatgccaacc
     2041 gtgtggtctt caatgaaatc accaaggatg cagtcaaaaa tgcttttaaa gaacctcgta
     2101 agatcgatat ggacttggtc gatgcccaac aagctcgtcg gatcttggac cgcttggtag
     2161 ggtattcgat ttcgcctatt ttgtggaaga aggtcaagaa gggcttgtca gcaggtcgcg
     2221 ttcagtccat tgcccttaaa ctcatcattg accgtgaaaa tgaaatcaat gccttccagc
     2281 cagaagaata ctggacagtt gatgctgtct ttaaaaaggg aaccaaacaa tttcatgctt
     2341 ccttctatgg agtagatggt aaaaagatga aactgaccag caatgacgaa gtcaaggaag
     2401 tcttgtctcg tctgacgagt aaagactttt cagtagatca ggtggataag aaagagcgca
     2461 agcgcaatgc tcctttaccc tataccactt catctatgca gatggatgct gccaataaaa
     2521 tcaatttccg tactcgaaaa accatgatgg ttgcccaaca gctctatgaa ggaattaata
     2581 tcggttctgg tgttcaaggt ttgattacct atatgcgtac cgattcgact cgtatcagtc
     2641 ctgtagcgca aaatgaggtg gcaagcttca ttacggatcg ttttggtagt aagtattcta
     2701 agcacggtag caaggtcaaa aacgcatcag gtgctcagga tgcccatgag gctattcgtc
     2761 cgtcaagtgt ctttaataca ccagaaagca tcgctaagta tctggacaag gatcagctca
     2821 agctatatac ccttatctgg aatcgttttg tggctagcca gatgacagcg gccgtttttg
     2881 ataccatggc tgttaaattg tctcaaaaag gggttcaatt tgctgccaat ggtagtcagg
     2941 ttaagtttga tggttatctt gccatttata atgattctga caagaataag atgttaccgg
     3001 acatggttgt tggagatgtg gtcaaacagg tcaatagcaa accagagcaa catttcaccc
     3061 aaccgcctgc ccgttattct gaagcaacac tgattaaaac cttagaggaa aatggggttg
     3121 gacgtccatc aacctacgcg ccaaccattg aaaccattca gaaacgttat tatgttcgcc
     3181 tggcagccaa acgttttgaa ccgacagagt tgggagaaat tgtcaataag ctcatcgttg
     3241 aatatttccc agatatcgta aacgtgacct tcacagctga aatggaaggt aaactggatg
     3301 atgtcgaagt tggaaaagag cagtggcgac gggtcattga tgccttttac aaaccattct
     3361 ctaaagaagt tgccaaggct gaagaagaaa tggaaaaaat ccagattaag gatgaaccag
     3421 ctggatttga ctgtgaagtg tgtggcagtc caatggtcat taaacttggt cgttttggta
     3481 aattctacgc ttgtagcaat ttcccagatt gccgtcatac ccaagcaatc gtgaaagaga
     3541 ttggtgttga gtgtccaagc tgtcatcagg gacaaattat tgagcgaaaa accaagcgta
     3601 atcgcctctt ctatggttgc aatcgctatc cagaatgtga atttacctct tgggacaagc
     3661 ctgttggtcg tgactgtcca aaatgtggca acttcctcat ggagaaaaaa gtccgtggtg
     3721 gtggcaagca ggttgtttgt agcaaaggcg actacgagga agaaaagatt aaataagagg
     3781 aggggcccga aagtaaattt caggctcctt ttggttagaa cttgacaaaa tacatcattt
     3841 tatgcgaaac tagaagaaga ttattttaac taggagaagt tatgcgtctt atctatctga
     3901 taattggttt tttatcactg accttggcta ttgttggggt tgttttaccc ttgttgccga
     3961 caacgccttt ccttttgttg tctattgctt gtttctccag aagttccaag cgtttcgaag
     4021 actggcttta tcataccaag ctctatcaaa catatgtagc tgattttcgc gagactaagt
     4081 ctattacgcg tgaacgaaag aaaaaaatca tcgtctctat ctacgtcttg atgggaattt
     4141 ctatttattt tgcacctctt ttaccagtca aaatcggtct gggtgctttg accatcttta
     4201 tcacttatta tcttttcaag gtcattccag acaaagaata gttaaaacag tagttatttg
     4261 ccttgataaa attgaaagca tattcataac aatatgatat aataaatttg aagtaatatt
     4321 caaggagaat caaatgattt acgaattttg tgctgaaaat gtgactttac ttgaaaaagc
     4381 gatgcaggct ggagctcgtc ggattgaact ctgtgataat ctagcagttg gtgggacaac
     4441 acccagctat ggagtgacta aggcagcggt tgaactggca gctaactacg atacaaccat
     4501 catgaccatg attcggccac gtggtggtga ctttgtctat aatgacctag aaattgctat
     4561 catgctagaa gacattcgtt tgactgctca ggctggaagt caaggggttg tatttggagc
     4621 tttaactgct gataaaaagt tggataagcc taatctggaa aagttaattg ctgcatcaaa
     4681 aggaatggaa attgtctttc acatggcctt tgatgaacta agtgatgaag atcaaccgga
     4741 agctattgac tggctcagtc aagccggtgt cactcgtatc ctaactcgtg ctggtgtgtc
     4801 tggcgactcc ttagaaaaac gttttgttca ctatcacaga attttggagt acgctaaagg
     4861 taaaattgaa attctaccag gtggggggat tgaccttgac aaccgtcaaa cctttatcga
     4921 ccagttgggg gtaacacaat tgcatggtac taaggttgtt ttttaaaaaa tagaaaggaa
     4981 ctgctagctt tgggtagcag ttttcactta tgtttgaaat ttttaaatcc tatcaattta
     5041 atcaagaaaa ggttcatgat tatggtttta tagaaaatag cgaagtctgg acatatagtt
     5101 gccagatttt gcaaggtgac tttgtcatga ctgtgtccat cactgctgat aatgtgaact
     5161 ttcaagtctt tgaccaagag actggtgacc tctatcctca cgttcatatg gaaagcatga
     5221 ggggaagttt tgtcggaaat gtccgtgagg cttgtctgga gattctttac cagattcgga
     5281 aggcttgttt tgatgtgcaa gattttatct gtcatcagac taagcgtatc atgactcaag
     5341 ttcaggaaaa gtatggaaac cagttggagt atctgtggga aaaatcgcct gatacagctg
     5401 tattgcgcca tgaaggcaat caaaagtggt atgccgtctt gatgaaaatc tcttggaata
     5461 agctggaaaa gggcagagaa ggacaagtgg aagcagtcaa cctcaagcat gaccaagtag
     5521 ctaatttgct ttcacaaaag gggatttatc cagccttcca tatgagcaag cgctactgga
     5581 ttagtgtgtc ccttgatgat actttatcag atgaagaagt actggaattg atagaaaaaa
     5641 gttggaactt aacctctaaa aaatgaaata ttttaataat tttcatgaac tttcaattag
     5701 ctaaatattc tttactgaag aaattttcag aaaatatagg atttaccaca ccagaggaat
     5761 gtggtgccat cttcaaatac ctgattgaga atgtcaagac ggatcgtcag atcatctatt
     5821 cgcctcactg tcatgatgac ctcggaatgg cagtggcaaa tagccttgct gctgtcaaga
     5881 atggtgcagg acgtgttgaa gggactatca atggtattag ggagcgagct gaaaatgctg
     5941 ctttggaaga aattgcagtg gctctcaata tttgccaaga ttactaccaa gtagaaacca
     6001 gtattgtcct aaatgagacc atcaatacgt cagaaatggt ttctcgcttc tctggtattc
     6061 cagttcctaa aaacaaagcc gtcgttggtg gcaatacctt ctcccacgaa tctggtattc
     6121 accaagatgg agtccttaaa aatcctctca cttatgagat catcacacct gaattggttg
     6181 gtgttaagat tccgcttgga aaattatctg gtcgccatgc ttttgttgag aaactgagag
     6241 aattggccct agattttaca gaagaggata tcaaaccact ctttgctaag ttcaaggcac
     6301 tggccgataa gaagtaagaa atcacagatg cagatactcg agctttggta gctggaacca
     6361 tggttgaaaa tccagaaggc ttccactttg atgatttaca acttcaaact catgcagata
     6421 atgacattga agcgctcgtt agcctagcca atatggatgg tgagaaagtc gaatttaatg
     6481 cgacagggca aggttccgtt gaagcaatct ttaacgctat cgataagttc tttaaccaat
     6541 ctgttcgttt ggtgtcctac actatcaatg cggtaacaga tggaatcgat gcccaggatc
     6601 gggttttggt cactgttgaa aacagagata cagaaaccat ctttaatgca gcagggcttg
     6661 attttgatgt gttgaaggct tctgctattg cctatataaa cgctaatacc tttgttcaaa
     6721 aagagaatgc aggtgagatg ggacgcagtg tttcttacca cgatatgcct agtgtgtaaa
     6781 ggagaaggct atggcaaaga aaatagtagc tctagtagga gacggaattg gcccagaaat
     6841 catggaggct ggtttagaag ttctggaggc tctagctgaa aaaacaggtt ttgactatga
     6901 gattgacaga cgaccgttcg gaggtgcaga tattgatgca gcaggacctc ccttacctga
     6961 tgaaaccctt aaggcaagta gggaagcaga tgctatccta ctagtagcta tcggtagtcc
     7021 tcagtatgat ggagtagcgg ttcgccctga acaaggcctg atggctctcc gtaaggaact
     7081 caatctttac gctaatattc gtcctgtaaa aatctttgac agtctcaagt atttgtcacc
     7141 actcaaaccg gaacgaattg ctggtgtaga ctttgtcgtg gtgcgtgaat tgacaggcga
     7201 gatttacttt ggagatcata ttcttgaaga gcgcaaagcg cgtgatatca acgactatag
     7261 ctatgaggaa gtggagcgga ttattcgcaa agcctttgaa attgcaagaa atcgcagaaa
     7321 aatccttact agtatcgata agcaaaatgt tctagcgacc tcaaaactct ggcggaaagt
     7381 agctgaggaa gtcgcacagg atttctcaga tgtaaccttg gaacaccagc tggtagactc
     7441 agctgctatg cttatgatta ccaatcctgc taagtttgat gttattgtaa cggagaatct
     7501 ttttggagat attttatctg atgaatcaag cgtcttatct ggtacacttg gggttatgcc
     7561 atcagccagt cattctgaaa atggaccaag tctctatgaa cctattcacg gttcagtacc
     7621 tgatattgca ggtcaaggaa ttgccaatcc tatttccatg attttatcag ttgtcatgat
     7681 gttgagagat agtttcggac gttatgagga tacagagcgt atcaaacgtg ctgttgagac
     7741 aagtctggcg gcaggaattt taacgagaga tataggaggt caggcttcaa caaaggaaat
     7801 gatggaagct attattgcaa ggttatgaag ttagacgaaa aaattactct agtccttttg
     7861 atttggaatg tcatcatttt cttgatttat ggcattgaca aattcaaggc aagaagaaga
     7921 gcttggcgca tcccagagaa aatcttactc attttagccc ttacttgtgg tggttttggg
     7981 gcctggttag ctggaatcac ttttcaccat aagactcgaa aatggtactt taaaacagtt
     8041 tggtttcttg ggatggtgac cacactagta gccttatatt ttatttggag gtaatggatg
     8101 gcagggtctt cgagggaaca cgctgcttgg gctctagcgg actatggttt taaggtcgtg
     8161 attgcaggat ctttcggtga cattcattac aataatgaac tcaataatgg catgttgcca
     8221 atcgttcagc ctagagaggt tagagagaaa ctagcccagc taaaaccaac cgaccaggta
     8281 actgtggact tggaacaaca aaaaatcatc tcaccagttg aagaattcac cttcgagata
     8341 gatagcaagt ggaaacataa actcctaaat agtttggatg atatcggtat taccttgcag
     8401 tatgaagagt tgattgctgc ttatgaaaaa caacgaccag cctactggca ggattagaaa
     8461 aaatagaaaa ggaaatatag taaactgaaa taagatgtaa acaaatgaat tggagcttaa
     8521 catccatttc cagcaatttt ttagaaacta cagtggacta ttctggattc aacacattat
     8581 aaaattatga caaaacacat tcacaagaag gctacgacat tttaaaaggt gagggcggat
     8641 gtatcgtttg ccctactaaa gttggttaca ttatcatgac cagcaacaag gcaggacttg
     8701 agcgtaagtt cgcagccaaa gaacgtaagc gtaacaaacc aggtgttgtt ctctgcggta
     8761 gcatggatga acttcgcgct ttagcgcaac tcaacccaga aattgaagca ttctactaaa
     8821 aacattggat gaagatattc ttcttggttg tatccttcct tggaaaccag aagcctttga
     8881 aaaactcaaa gcatacgggg atggccgtga agaacttatg actgatgtac gtggtactag
     8941 ctgttttgtt atcaagtttg gaaaagcagg tgaacaattg gctgccaagc tttgggaaga
     9001 aggtaaaatg gtctacgcct catatgcttc aatgacaaaa cgattgaaac tcgctatgag
     9061 agcaaggtgt aatggtgtct atggtcgata aggacggcaa actcatccca gaacaaggag
     9121 gagcacgttc aacttcacca gcaccagttg tgatccgtaa agggcttgac attgataaaa
     9181 tcatgatgca cctgtcagat acttttaact catgggacta ccgtcaggtt gagtattatt
     9241 aagataaaaa aagaagtcta gtgttatgag atattaaagc ttctaacact ggactttttg
     9301 tttagaattt cttt
//
LOCUS       DAVHIU010000075         9192 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVHIU010000075 DAVHIU010000000
VERSION     DAVHIU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.077"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            432..1907
                     /locus_tag="VVN06_001481"
     CDS             432..1907
                     /locus_tag="VVN06_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5562997.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMAAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1873..2588)
                     /locus_tag="VVN06_001482"
                     /pseudo
     CDS             complement(1873..2588)
                     /locus_tag="VVN06_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2675..2812
                     /locus_tag="VVN06_001483"
     CDS             2675..2812
                     /locus_tag="VVN06_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5562998.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            2843..3703
                     /gene="speE"
                     /locus_tag="VVN06_001484"
     CDS             2843..3703
                     /gene="speE"
                     /locus_tag="VVN06_001484"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU5562999.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3700..4959
                     /locus_tag="VVN06_001485"
     CDS             3700..4959
                     /locus_tag="VVN06_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU5563000.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4959..6086
                     /gene="nspC"
                     /locus_tag="VVN06_001486"
     CDS             4959..6086
                     /gene="nspC"
                     /locus_tag="VVN06_001486"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU5563001.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6083..7168
                     /gene="aguA"
                     /locus_tag="VVN06_001487"
     CDS             6083..7168
                     /gene="aguA"
                     /locus_tag="VVN06_001487"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU5563002.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLYEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEVLEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGSAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7178..8053
                     /gene="aguB"
                     /locus_tag="VVN06_001488"
     CDS             7178..8053
                     /gene="aguB"
                     /locus_tag="VVN06_001488"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU5563003.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8234..9043)
                     /locus_tag="VVN06_001489"
     CDS             complement(8234..9043)
                     /locus_tag="VVN06_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU5563004.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2689 a         1773 c         2068 g         2662 t
ORIGIN      
        1 aatactttac aagttcttaa aaacatgata tagtaataaa gcttagaaaa tgagatgatg
       61 ttttctagca aatataaacc cgagtaaaaa atgcctacgg acaggcaggg ttgaatgccg
      121 aagcgtggtt gaaaagccac attattgata gggttaaaag cctactttta taagttgatg
      181 ttaggacact tgttctaatt cataaatttt tagtgtggtg aaagcacacg tcatcttgtg
      241 aaacgatcaa taaagtacgt aatatttgct actagagagt taggaaacat cgggaacaga
      301 catactcaac agaaaccaaa ataaacacgt cagaagattg cagagcaggt gaaaacctgc
      361 tcttttttca tgagtcaacc tttagttcct tagttttcat aaggtcctaa aaatattgaa
      421 aggagtatgt tttgaaagag ttagatcaaa accaagcccc aatttatgag gccttggtga
      481 agttacgcaa gaaaaggatt gttccctttg atgttccagg tcacaagcgt ggacggggaa
      541 atccagaact tgtcgaactc ttaggagaaa aatgtgtagg cattgatgtc aattcgatga
      601 aacccttgga taatttaggc catcctattt cgattattcg tgatgcagag gagctggctg
      661 cagatgcttt tggagctagc catgcctttc taatgattgg tggaacaact tcatcggtgc
      721 agactatgat tctggcaacc tgcaaggcag gagataagat tattctgcca cgaaatgtcc
      781 ataaatctgc tatcaatgcg ttggttctat gtggtgccat tcccatctat atcgagatga
      841 gtgtagatcc taagattggt atcgctttag gtcttgaaaa tgaccgagta gcacaggcca
      901 taaaggacca tccagatgct aaggctatcc taatcaacaa tcctacttac tacggcatct
      961 gttcagacct aaaggggttg acagaaatgg ctcatgaagc tggcatgatg gttttagtag
     1021 atgaagccca tggagcgcat ttgcatttca ctggtaaact tccaatttct gctatggctg
     1081 caggagctga tatggcagca gtttccatgc ataagtctgg tgggagtttg acacaaagtt
     1141 cgcttctttt aatcggggag cagatgaatc ccgagtacgt tcgtcagatt ataaacctga
     1201 cccagtctac atctgcctct tacttattga tggctagttt ggatatttca cgtcgcaact
     1261 tggcccttcg tggtaaagag tcgtttgagg aagtcattga gctatccgag tacgctcgtc
     1321 atgaaatcaa tgccattggc ggttactatg cttactcaaa agagttaata gatggtgtgt
     1381 cggtctgtga ttttgatgta accaagttgt cagtttacac tcagggaata ggcttaacag
     1441 gtatcgaggt ttatgacctc ttgcgagacg aatacgacat tcagattgag tttggtgata
     1501 tcggcaatat cttggcctat atttccatcg gcgaccgcat ccaagacatc gagcgcttgg
     1561 ttggtgctct ggctgatatt aagagactct attcacgaga tgggaaggac ttaatagctg
     1621 gagaatatat tcagcccgag ttggtgctgt ctccacaaga agccttctat tcagagagaa
     1681 aaagtttgac cttagatgag tctgttggac aggtctgtgg agaatttgtc atgtgctatc
     1741 ctccagggat tcctatcttg gctcctggtg aacgtattac acgagaaatt gtagactata
     1801 tccaattcgc caaggaacgt ggttgctccc tccaagggac ggaagatcca gaggtcaatc
     1861 atatcaacgt tattaagaga aagacaaact ataagaaaag tcaatagttt tatctaaact
     1921 atttcttatt tcaatttgat gatttggcga tgattttaga gcacggcaaa aagcccttga
     1981 attagaagcg gtcaatcgct taatttctat cagcttatca aatcctgcct caatcctttt
     2041 ctgaggatta gggtagcgtg tcaagagttg gtaggtatat tctgaatgct ttccaacgat
     2101 tttatccaac tcaggaaaga tgatatcaag acaacgagtg tattgtactt tccaatcaga
     2161 ctgtttttct tgagacgatg aatatgtcta gccagtattt ttaggtctac ttgccgatta
     2221 tcgtgttgaa attgttcacg attggggtca gaaagaagtt taagagcgat gccatgagcg
     2281 tctttcttat ccgttttagt cttgcgaagt gataatgatt tggcaaattc cttgatgagc
     2341 aaaggattgt aggtgtaaac tttatatcct tgttcatgca ggaagttcag tagattaaag
     2401 gcataatgtc cagtatcttc aagagcgatg agacagtctt ggttgagctg tcgaagagac
     2461 agatctaaga gttcaaaacc agctttatta tttgaaaaag tgagtggttt aagaacagtt
     2521 tttccttgaa cattcaaggc tgtaacatcg tgtttatttt tagcgacatc aatgcccaca
     2581 taaagcatgg gagtacctcc agatgtagta tttcaagtct acttggttat ccacgaactt
     2641 tttgccttgt taccttagac gagataaaac gtctatgcgt tatcaaactc attaccaatt
     2701 gaaacaaaag ctgtggttag agcctttcgg aaatcgtcaa gcgattggag gaaatgaact
     2761 aatccacagt ggcttattcc aagtatacca cttgggcttt ggcagtagct aactgcgcta
     2821 aatataatat aaggagaaat agatggattt atggttttct gaagttcata ctccagatgt
     2881 caaattgtct ctgagaacag ccaagcaact ttacgctgga aaaagtgaat ggcaggatat
     2941 cgaagtcttg gatacgccag cttttgggaa aatactgatt ttaaatggcc atgtcttgtt
     3001 ctcagatgcg gatgatttcg tctacaatga aatgaccgtt cacgttccca tggctgtcca
     3061 cccaaatcca aagaaagtat tggttattgg gggtggtgac ggcggtgttg cccaagtatt
     3121 aacactctat cctgaactgg aacaaatcga tatcgtggaa ccggatgaga tgttggtcga
     3181 ggtctgtcgt gagtatttcc cagactttgc tgcagggcta gatgatcctc gtgttaccat
     3241 ttactaccaa aatgggctac gctttttgcg aaactgcgaa gatgattacg atattatcat
     3301 caacgatgcg acagatccat ttggccatac ggaaggactc tttaccaagg aattctacgg
     3361 caatagttat cgagctctga aggaagacgg catcatgatt taccagcatg ggagtccctt
     3421 ctttgacgag gatgagtcgg cctgccgaag catgcaccgc aaggtcaatc aagcctttcc
     3481 aatcagtcgg gtttatcagg cccatattcc aactagccca gctggctatt ggttgtttgg
     3541 atttgcatcg aaaaaatacc accctgtcaa agattttgac aaggaaggtt ggaaaaaacg
     3601 ccagcttttc acagaatact acactgcaaa cttacacgtg ggagccttta tgttgcccaa
     3661 gtatgttgag gacattttag aagaagagga aggaaaaaaa tgagtcgttt actagttatt
     3721 ggttgtgggg gcgttgccca agttgctatt tcaaaaattt gtcaagatag cgaaacattt
     3781 acagagatta tgattgctag ccgtaccaag tcaaaatgcg atgacttgaa agcgaagcta
     3841 gaaggcaaaa caagtactaa aattgaaact gcagcacttg atgctgacaa ggtagaagag
     3901 gtcattacct tgattgaaag ctacaaacca gaagctgttt tgaatgtagc tctgccttat
     3961 caagatttaa ccattatgga tgcttgtttg gcaacaggtg ttcactatat cgatacagcc
     4021 aactacgaag cagaagacac agaagaccct gagtggcgtg ctatctacga aaaacgttgt
     4081 aaggaacttg gttttacagc ctactttgac tactcatggc agtgggctta tcaagagaaa
     4141 ttcaaagaag caggcttgac tgctcttctt ggttctggtt ttgacccagg tgtaactagt
     4201 gtcttttcag cttatgccct caaacactat tttgatgaaa tccattatat cgacatttta
     4261 gactgtaatg gcggtgacca cggttatcca tttgcaacca actttaatcc agaaattaat
     4321 ctccgtgagg tttctgcgcc aggttcttac tgggaagatg ggaaatgggt cgaagtcgaa
     4381 gctatgtcta tcaagcgtga gtatgatttc cctcaagttg gacaaaaaga tatgtatctc
     4441 cttcaccatg aagaaatcga atcattggcc aagaacattc caggtgtcaa acgcattcgt
     4501 ttctttatga cttttggtca atcttacttg acgcacatga aatgtcttga aaatgttgga
     4561 ctccttcgta cggataccat taactttaac ggccaagaaa ttgttccaat tcaatttttg
     4621 aaagccttgc ttccagatcc tgctagcctt ggcccacgta ctgtaggtaa aaccaatatt
     4681 ggatgtatct ttacaggtgt caaagatggc gttgaaaaga ctatctacat ctacaatgtt
     4741 tgcgaccatc aagagtgcta tgcagaagtc gggtcacaag caatttcata cacgacaggc
     4801 gttccagcca tgattgggac aaaattagtc atgaacggaa cttggaaaca agctggagtg
     4861 tataaccttg aggagttaga tccagatcca ttcatggaag ctttgaatga gtatggtttg
     4921 ccatgggttg tggttgaaaa tccacaaatg gtggactaat gaagttagaa caagtaccaa
     4981 caccagccta tgttattgac ttggccaagt tagaagctaa ttgccgcatt ctacaatatg
     5041 tacaagaaga ggtcggttgc aaggtcttgc ttgcccagaa ggcatattcc ctctacaaaa
     5101 cttatccctt gattagccag tatctatcag gtacgacagc tagtggactc tatgaggcca
     5161 aattggcaag ggaagaattt cctggtgaag tccatgtatt tgcgcctgct ttcaaggatg
     5221 cagacttgga ggaattgctt gggataacag accatatcgt ctttaactca gagagacagt
     5281 tgcgtaagca tggggctcgt tgtcgagagg ctggtgtcag tgttggtttg cgcctcaacc
     5341 ctcagtgttc aactcaagga gatcacgcgc tctatgaccc ttgtgcacct ggttctcgct
     5401 ttggagttac tatagacaag attccgagtg atttgctaga tttggttgac ggacttcatt
     5461 ttcataccct ttgcgagcag ggagcagatg atttacaaac aactttgaaa gcagtagaag
     5521 aacagtttgg tccctactta catgaggtaa aatggctcaa tatgggtggt ggtcatcata
     5581 ttacaagaga aggttacgat gtggatttgc tgatttcaga aatcaagcgt atccgaaaaa
     5641 cttacaatct tgaaatctat atcgagcctg gtgaagccat tgcgcttaat gcgggttatt
     5701 tagcaactga ggtattagat attgtagaaa acggtatgga aatcttggtt ttagacgcct
     5761 ctgcgacctg ccatatgcct gatgtacttg agatgcccta tcgtccacct ttgagaaatg
     5821 gctttgagtc acaggaaaaa gcccatacct acagactttc ttctaatacc tgtctgacgg
     5881 gcgatgtgat tggtgattat agttttgaaa atccagtcca aatcggagac agactttatt
     5941 ttcaagacat ggccatttat tcttttgtca aaaataatac ctttaatggt attggattgc
     6001 caagtctcta tctcatggac gaacagggag actgtagctt actcaaagct tttggctatc
     6061 aagactttaa agggagatta tcatgatgga cagtccaaaa aaattaggct atcacatgcc
     6121 agcagagtac gaaccccatc atggtaccct catgatatgg ccgactcgac caggatcatg
     6181 gccttttcaa ggaaaggctg ctaaaagagc atttactcag atcatcgaga ccatagcaga
     6241 aggggaaaga gtctatcttt tggtggagca ggcctatcta tatgaagccc aatcctatct
     6301 tggagacaag gttgtttatt tagacattcc caccaatgat gcctgggcgc gtgatactgg
     6361 cccaaccatt ctcgtcaatg ataaaggtaa gaaattagcc gtggattggg ccttcaatgc
     6421 ttggggaggc acctatgatg gtctttatca agattatgaa gaggatgacc aagtagccag
     6481 tcgttttgct gaggtcttgg aaaagcctgt ctatgatgct aaaccttttg tactggaagg
     6541 aggcgcaatc catagcgatg gtcaaggaac tattctcgta actgaaagtt gcttgcttag
     6601 tcctggtcgc aatcctaacc tgactaaaga ggagattgaa aacacattat tagaaagtct
     6661 tggtgctgaa aaagttattt ggcttcctta tggtatttat caggatgaaa ccaatgaaca
     6721 cgtcgataat gttgctgcct ttgttggttc tgctgagctt gttttggctt ggacagatga
     6781 cgaaaatgat ccccagtatg ccatgtcaaa agcagatctc gaactcttag aacaggaaac
     6841 agatgcaaaa ggttgtcact tcaccattca taaattgcct atccctgcag ttcgacaagt
     6901 tgtgacagaa gaagatttgc caggctacat ctatgaagaa ggagaagaag agcgatacgc
     6961 aggtgaacga ctagcagctt cctacgtaaa cttttatatc gccaacaagg ctgtcttggt
     7021 tccacagttt gaggatgtaa acgaccaagt ggccatagat atcctcagca agtgtttccc
     7081 agaccgtaaa gttgtcggaa taccagccag agatattctc ttaggtggtg gcaatatcca
     7141 ctgtatcacc caacaaattc cagaatagga gaaaaagatg agaaatgtaa gagttgcaac
     7201 cattcagatg caatgcgcta aggatgtggc aacaaatatc caaaccgcag agcgtttagt
     7261 acgtcaggct gctgagcaag gagcccaaat tattctcttg cccgagttgt ttgaacatcc
     7321 ctatttctgt caggaacgtc agtatgacta ctaccagtat gcccaatctg tagcggaaaa
     7381 tactgcaatt cagcatttta aggtgattgc taaggaacta caagttgttt taccaatcag
     7441 tttctatgaa aaagatggta atgtcttgta taactctatt gccgtcattg atgcagatgg
     7501 ggaagtgctg ggcgtttatc gaaagaccca cataccagat gaccattatt atcaagaaaa
     7561 attctatttc acgcctggta acactggttt caaggtctgg aatactcgct atgctaagat
     7621 tggtatcggt atctgttggg atcaatggtt ccctgaaaca gcgcgctgtc ttgcattgaa
     7681 tggtgctgaa ttgctctttt atcctacagc tatcggttca gagccaattt tggatacaga
     7741 tagttgtggt cactggcaac gtactatgca agggcacgca gcagcgaata ttgttccagt
     7801 catcgcagcc aatcgttatg gtttagagga ggttactcct agtgaggaaa atggcggaca
     7861 gagttccagt cttgacttct acggttcctc ctttatgacg gatgaaacag gagctattct
     7921 agaacgagct gaaagacaag aagaagctgt tctgttagct acttatgacc tagacaaggg
     7981 agcaagtgaa cgcctaaact ggggcttgtt tcgagataga agaccagaaa tgtatagaca
     8041 aattacagat tagtgtggga gaaatgagag attcattctg ctagactaac ttcttattag
     8101 caactataag atactatggc atctagtaaa tcgattttta tgattcgcta ttcttgtcta
     8161 ttgattagtc cgtattttaa aatattagca aaaaagcaaa tagcagtaac ttctgtctat
     8221 ttgcttttct tttttataga atatatttct caatagcacg cgcaacgccg tcttcttcgt
     8281 tgcttgaggt aacggcatcc gcaagagatt tgacataatc gctggcattt cccattgcaa
     8341 tcccaagccc tgcaaactgg agcatttcga tatcgttatt agcatcgccc atggccataa
     8401 tctctgagga atcaatcttc aaaatctcag ctagtcgtga aagagcagta gcctttgtcg
     8461 ttccaagcgg cattgcttca taaatgacag gctgcgaacg aactccactg aatcgttggc
     8521 aaagctcttc agcaaaacgc tgctcaaaat cgtctgtttg ttcttttgtt cctaaaaaca
     8581 taccttggaa catccggaac tttccactag tcgcttcttc aagagaaatt tcagtcaggt
     8641 ctgaaaatac tagtttagca tcattttcaa taacttgatt gggtttgcca ccgagaacaa
     8701 aataatgtga ctcgtcaaaa agtgtcaact gaacatcact cttttcagca aggtcataga
     8761 ggtattcgat gtcagctgga ctcagttctt tccagtcaac tagactccaa tcactggtct
     8821 ggtgagttga acaaccgttg ttaacaataa tatattcgtt ctggaggtca agctccagtt
     8881 ttttgtagta ggggaggaca ccgaaaaggg ggcgacccgt acagagaacc agtttgacac
     8941 ctttttcaat ggctttgtga atagcagtaa tgtgagcttg tgggatttcc ttggcttcat
     9001 tgaggagggt gccgtccata tccaaggcta gtagtttaat cataggtctt ccttctttat
     9061 cttttgctat tattatagca tatttttgag aagaaattga tagaaagctt gagactaatt
     9121 gattttatag tttaagatgt tttgatgaca attcatgatt tgaagaggat atttcgcaaa
     9181 gatatgctat ac
//
LOCUS       DAVHIU010000076         9158 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVHIU010000076 DAVHIU010000000
VERSION     DAVHIU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.078"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            126..5066
                     /locus_tag="VVN06_001490"
     CDS             126..5066
                     /locus_tag="VVN06_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU5563005.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5156..6352
                     /locus_tag="VVN06_001491"
     CDS             5156..6352
                     /locus_tag="VVN06_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5563006.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6488..7015
                     /locus_tag="VVN06_001492"
     CDS             6488..7015
                     /locus_tag="VVN06_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU5563007.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7092..7448
                     /gene="glnR"
                     /locus_tag="VVN06_001493"
     CDS             7092..7448
                     /gene="glnR"
                     /locus_tag="VVN06_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU5563008.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7485..8831
                     /gene="glnA"
                     /locus_tag="VVN06_001494"
     CDS             7485..8831
                     /gene="glnA"
                     /locus_tag="VVN06_001494"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5563009.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8927..9046)
                     /locus_tag="VVN06_001495"
     CDS             complement(8927..9046)
                     /locus_tag="VVN06_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU5563010.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         2948 a         1680 c         2022 g         2508 t
ORIGIN      
        1 ctattgtttt ttgtctaaaa atgttttagg ataaaaatgt gattatcatg attctagagt
       61 taaaaaacta cagaatataa agcgctttct tgaaaacaac aaaatcaatc ttttaggagg
      121 agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt aagttatcag
      181 taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc ttggctgaag
      241 aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga gagagtgagc
      301 attcgaccaa tgaagctgat aagcagaatg aaggggaaca tactagagaa aacaagctag
      361 aaaaggcaga aggagtagcg acagcatctg aaactgcaga agcagctagc gcagctaaac
      421 cagaggaaaa agcaagtgag gtggttgcag aaacaccatc tgcagaagca aaacctaagt
      481 ctgacaagga aacagaagca aagcccgaag caactaacca aggggatgag tctaagccag
      541 cagccgaagc taataagact gaaaaagaag tccagccaga tgtccctaaa aatacagaaa
      601 aaacattaaa accaaaggaa atcaaattta attcttggga agaattgtta aaatgggaac
      661 caggtgctcg tgaagatgat gctattaacc gcggatctgt tgtcctcgct tcacgtcgga
      721 caggtcattt agtcaatgaa aaagctagca aggaagcaaa agttcaagcc ttatcaaaca
      781 ccaattctaa agcaaaagac catgcttctg ttggtggaga agagttcaag gcctatgctt
      841 ttgactattg gcaatatcta gattcaatgg tcttctggga aggtctcgta ccaactcctg
      901 acgttattga tgcaggtcac cgtaacgggg ttcctgtata cggtacactc ttcttcaact
      961 ggtctaatag tattgcagat caagaaagat ttgctgaagc tttgaagcaa gacgcagatg
     1021 gtagcttccc aattgcccgt aaattggtag acatggccaa gtattatggc tatgatggct
     1081 atttcatcaa ccaagaaaca actggagatt tggttaaacc tcttggagaa aagatgcgcc
     1141 agtttatgct ctatagcaag gaatatgctg ctaaggtaaa ccatccaatc aagtattctt
     1201 ggtacgatgc catgacctat aactatggac gttaccatca agatggtttg ggagaataca
     1261 actaccaatt catgcaacca gaaggagata aggttccggc agataacttc tttgctaact
     1321 ttaactggga taaggctaaa aatgattaca ctattgcaac tgccaactgg attggtcgta
     1381 atccttatga tgtatttgca ggtttggaat tgcaacaggg tggttcctac aagacaaagg
     1441 ttaagtggaa tgacatttta gacgaaaatg ggaaattgcg cctttctctt ggtttatttg
     1501 ccccagatac cattacaagt ttaggaaaaa ctggtgaaga ttatcataaa aatgaagata
     1561 tcttctttac aggttatcaa ggagacccca ctggccaaaa accaggtgac aaagattggt
     1621 atggtattgc taacctagtt gcggaccgta cgccagcggt aggtaatact tttactactt
     1681 cttttaatac aggtcatggt aaaaaatggt tcgtagatgg taaggtttct aaggattctg
     1741 agtggaatta tcgttcagta tcaggtgttc ttccaacatg gcgctggtgg cagacttcaa
     1801 caggggaaaa acttcgtgca gaatatgatt ttacagatgc ctataatggc ggaaattccc
     1861 ttaaattctc tggtgatgta gccggtaaga cagatcagga tgtgagactt tattctacta
     1921 agttagaagt aactgagaag accaaacttc gtgttgccca caagggagga aaaggttcta
     1981 aagtttatat ggcattctct acaactccag actacaaatt cgatgatgca gatgcatgga
     2041 aagagctaac cctttctgac aactggacaa atgaagaatt tgatcttagc tcactagcgg
     2101 gtaaaaccat ctatgcagtc aaactatttt tcgagcatga aggtgctgta aaagattatc
     2161 agtttaacct aggacaatta actatctcgg acaatcacca agagccacaa tcgccgacaa
     2221 gcttttctgt agtgaaacaa tctcttaaaa atgcccaaga agcggaagca gttgtgcaat
     2281 ttaaaggcaa caaggatgca gatttctatg aagtttatga aaaagatgga gacagctgga
     2341 aattactaac tggctcatct tctacaacta tttatctacc aaaagttagc cgctcagcaa
     2401 gtgctcaggg tacaactcaa gaactgaagg ttgtagcagt cggtaaaaat ggagttcgtt
     2461 cagaagctgc aaccacaacc tttgattggg gtatgactgt aaaagatacc agcctaccaa
     2521 aaccactagc tgaaaatatc gttccaggtg caacagttat tgatagtact ttccctaaga
     2581 ctgaaggtgg agaaggtatt gaaggtatgt tgaacggtac cattactagc ttgtcagata
     2641 aatggtcttc agctcagttg agtggtagtg tggatattcg tttgaccaag ccacgtaccg
     2701 ttgttagatg ggtcatggat catgcaggag ctggtggtga gtctgttaac gatggcttga
     2761 tgaacactaa agactttgac ctttattata aagatgcaga tggtgagtgg aagctagcta
     2821 aggaagtccg tggtaacaaa gcacacgtga cagatatcac tcttgataaa ccaatcactg
     2881 ctcaagactg gcgcttgaat gttgtcactt ctgacaatgg aactccatgg aaggctattc
     2941 gtatctataa ctggaaaatg tatgaaaagc ttgatactga gagtgtcaat attccgatgg
     3001 ccaaggctgc agcccgttct ctaggcaata acaaggtaca agttggcttt gcagatgtac
     3061 cggctggagc aactattacc gtttatgata atccaaattc tcaaactccg ctcgcaacct
     3121 tgaagagcga agttggagga gacctagcaa gtgcaccatt ggatttgaca aatcaatctg
     3181 gtcttcttta ttatcgtacc cagttgccag gcaaggaaat tagtaatgtc ctagcagttt
     3241 ccgttccaaa agatgacaga agaatcaagt cagtcagcct agaaacagga cctaagaaaa
     3301 caagctacgc cgaaggggag gatttggacc ttagaggtgg tgttcttcga gttcagtatg
     3361 aaggaggaac tgaggacgaa ctcattcgcc taactcacgc aggtgtatca gtatcaggtt
     3421 ttgatacgca tcataaggga gaacagaatc ttactctcca atatttggga caaccagtaa
     3481 atgctaattt gtcagtgact gtcactggcc aagatgaagc aagtccgaaa actattttgg
     3541 gaattgaagt aagtcaggaa ccgaaaaaag attacctagt tggtgatagc ttagacttgt
     3601 ctgaaggacg ctttgcagtg gcttatagca atgacaccat ggaagaacat tcctttactg
     3661 atgagggagt tgaaatttct ggttacgatg ctcaaaagac tggtcgtcaa accttgacgc
     3721 ttcgttacca aggtcatgaa gtcaactttg atgttttggt atctccaaaa gcagcattga
     3781 acgatgagta cctcaaacaa aaattagcag aagttgaagc tgctaagaac aaggtggtct
     3841 ataactttgc ttcaccagaa gtaaaagaag ctttcttgaa agcaattgaa gcggccgaac
     3901 aagtgttgaa agaccatgaa actagcaccc aagatcaagt caatgaccga cttaataaat
     3961 tgacagaagc tcataaagct ctgaatggtc aagagaaatt taaggaagaa aagacagagc
     4021 ttgatcgttt aacaggtgag gttcaagaac tcttggctgc caaaccaaac catccttcag
     4081 gttctgccct agctccgctt cttgagaaaa acaaggcctt ggttgaaaaa gtagatttga
     4141 gtccagaaga gcttgcaaca gcgaaacaga gtctaaaaga tctggttgct ttattgaaag
     4201 aagacaagcc agcagtcttt tctgatagta aaacaggtgt tgaagtacac ttctcaaata
     4261 aagagaagac tgtcatcaag ggtttgaaag tagagcgtgt tcaagcaagt gctgaagaga
     4321 agaaatactt tgctggagaa gatgctcatg tctttgaaat agaaggtttg gatgaaaaag
     4381 gtcaagatgt tgatctctct tacgcttcta ttgtgaaaat cccaattgaa aaagataaga
     4441 aagttaagaa agtatttttc ttacctgaag gcaaagaggc agtagaattg gcttttgaac
     4501 aaacggatag tcatgttatc tttacagcac cacactttac tcattatgcc tttgtttatg
     4561 aatctgctga aaaaccacaa cctgctaaac cagcaccaca aaacaaagtc cttccaaaac
     4621 ctacttatca accggcttct gatcaacaaa aggctcctaa attggaagtt caagaggaaa
     4681 aggttgcctt tcatcgtcaa gagcatgaaa atactgagat gctagttggg gaacaacgag
     4741 tcatcataca gggacgagat ggactgttaa gacatgtctt tgaagttgat gaaaacggtc
     4801 agcgtcgtct tcgttcaaca gaagtcatcc aagaagcgat tccagaaatt gttgaaattg
     4861 gaacaaaagt aaaaacagta ccagcagtag tagctacaca ggaaaaacca gctcaaaata
     4921 cagcagttaa atcagaagaa gcaagcaaac aattgccaaa tacaggaaca gctgatgcta
     4981 atgaagccct aatagcaggc ttagccagcc ttggtcttgc tagtttagcc ttgaccttga
     5041 gacggaaaag agaagataaa gattaaatat cgaaaaatct tgtgaaatct ttccttatat
     5101 ttccaaagtg tgatataata gtttcgaata aaataaataa aggagttttt gtaacatggc
     5161 aaaacttact gttaaagacg ttgacttgaa aggtaaaaaa gtcctcgttc gtgttgactt
     5221 caacgtacca ttgaaagatg gcgtaatcac taacgataac cgtatcacag cagctcttcc
     5281 aactattaag tacatcatcg aacaaggtgg acgtgcaatt cttttctctc accttggacg
     5341 tgtgaaagaa gaagctgata aagctggtaa atcacttgct cctgtagcag ctgacttggc
     5401 agcaaaactt ggtcaagatg ttgttttccc aggtgtcact cgtggtgctg aattggaagc
     5461 ggcaatcaac gctcttgaag atggacaagt tctcttggtt gaaaacactc gttacgaaga
     5521 tgttgacggc aagaaagaat ctaaaaacga tcctgaactt ggtaaatact gggcatcact
     5581 tggagatggt atcttcgtaa acgatgcatt cggtacagct caccgtgcac acgcatctaa
     5641 cgttggtatc tcagcaaacg ttgaaaaagc agttgctggt ttccttcttg aaaacgaaat
     5701 tgcctacatc caagaagcag ttgaaactcc agaacgtcca ttcgtggcta tccttggtgg
     5761 ttcaaaagtt tcagacaaga tcggtgttat cgaaaacttg cttgaaaaag ctgataaagt
     5821 ccttatcggt ggtgggatga cttacacatt ctacaaagca caaggtatcg aaatcggtaa
     5881 ctcacttgta gaagaagaca aattggatgt tgcgaaagct cttcttgaaa aagcaaatgg
     5941 taaattgatc ttgccagttg actcaaaaga agctaacgca tttgctggtt acactgaagt
     6001 gcgtgacact gaaggtgaag cagtttctga aggcttcctt ggtcttgaca tcggtccaaa
     6061 atctatcgcc aaatttgacg aagctttgac tggtgccaaa acagttgtat ggaacggacc
     6121 tatgggtgta tttgaaaacc cagatttcca agctggtaca atcggtgtga tggacgctat
     6181 cgtgaaacaa ccaggagtta aatcaatcat cggtggtggt gactcagctg ccgcagcgat
     6241 taaccttggc cgtgcagaca agttctcatg gattagtacg ggtggtggag catcaatgga
     6301 acttcttgaa ggtaaggttc ttccaggact tgcagccttg acagaaaaat aagattttat
     6361 aaacaaatca aagaagagag ggatgaaagt tcctcttttc ttttgcttaa aataaaaacg
     6421 cttcctctca actattactc ataaaaatca ccgatttatg ataaaatgga aatagaaagt
     6481 tgagattatg agttatttta aaaaatataa attcgataaa tcccagttca aacttggtat
     6541 gcgaaccttt aaaacaggta ttgctgtttt tctagttctc ttgatttttg gcttttttgg
     6601 ctggaaaggt cttcaaattg gtgctttgac agccgttttt agcctgaggg agagttttga
     6661 tgagagtgtt cattttggga cttcgcgtat tctaggaaat agtatcggtg gactctatgc
     6721 cttggtcttc ttcttattaa atactttttt ccacgaagcc ttttgggtga ccttggtagt
     6781 tgttccaatc tgcaccatgt taaccattat gacaaatgta gccatgaata acaaagcagg
     6841 ggttattggt ggtgtagcag ctatgttaat cattacccta tcaattccaa gtggtgagac
     6901 aattttgtac gtgtttgtgc gtgtattaga aacgtttatg ggagtttttg tcgcaattat
     6961 cgtaaattac gatattgatc gtattcgtct ctttttagag aaaaaagaaa aataatgtta
     7021 cattttataa cattatcaat tgacgtttgt ctttttttag actataatag acagaaagaa
     7081 ggaaattgta aatgaaggaa aaagaatttc gccgaaatat ggctgttttt cctatcggca
     7141 gtgttatgaa gttgaccgat ttatcggcgc gtcagattcg ttattatgaa gatcaagagt
     7201 tgatcaagcc cgatcgaaac gaaggaaatc gtcgcatgta ttccttgaat gacatggatc
     7261 gtctgcttga aatcaaagat tatatctctg aaggttataa tatcgctgcc attaagaaaa
     7321 aatatgctga acgtgaagcg aaatccaaga aagcggttag tcagactgaa gtacgtcgtg
     7381 cacttcacaa tgaactcctc caacaggggc gttttgcttc agtacagtca ccttttggtc
     7441 gcggttaggc aaccgcaagt agtcatagat taaggagaaa acttatgcca atcacagctg
     7501 cagatattcg tcgtgaagtc aaggaaaaaa atgttacctt tattcgtctt atgttctcag
     7561 atattttggg aaccatgaaa aacgtcgaaa ttcctgctac agatgaacag ttagataagg
     7621 tcttgtcgaa caaggttatg tttgatggat cttctattga aggttttgta cgtatcaatg
     7681 agtcggatat gtacttgtac ccggacttgg atacatggac agtcttccct tggggagatg
     7741 aaaatggaag tgttgcaggt ctgatctgtg atgtttatac aacagaaggt gaaccatttg
     7801 cgggtgaccc tcgtggtaat ttgaaacgag ctcttcgtca catggaagaa gttggattca
     7861 aatccttcaa ccttggtcca gagccagaat tcttcctatt taagttggat gaaaatgggg
     7921 acccaacact tgaagtgaat gacaagggtg gctactttga tttggcacct actgaccttg
     7981 cggacaacac acgtcgtgag attgtgaatg tcttgaccaa aatgggattt gaagtagaag
     8041 cgagtcacca cgaggttgcg gttggacagc atgagattga ctttaagtac gatgaagttc
     8101 tccgtgcttg tgataagatt caaatcttta aacttgttgt taaaaccatt gctcgcaaac
     8161 acggacttta cgcaacattt atggcgaagc caaaatttgg tattgctggc tcaggtatgc
     8221 actgtaatat gtccttgttt gatgcagaag gaaataacgc cttctttgat ccaaatgatc
     8281 caaaaggaat gcagttgtca gaaacagctt accatttcct aggcggtttg atcaagcatg
     8341 cttacaacta tactgccatc atgaacccaa cagttaactc atacaaacgt ttggttccag
     8401 gttatgaagc gcctgtttac attgcttggg ctggtcgtaa ccgttcgcca cttgtgcgcg
     8461 tacctgcttc acgtggtatg ggaactcgtc ttgagttgcg ttcagtggat ccaatggcga
     8521 acccttacgt tgctatggct gttcttttgg aagttggttt gtatggtatt gaaaataaaa
     8581 tcgaagcacc agctcctatc gaagaaaata tctacatcat gacagcagaa gagcgcaagg
     8641 aagctggtat tacagacctt ccatcaactc ttcacaacgc tttgaaagct ttgacagaag
     8701 atgaagtggt taaagctgct ctcggagatc acatctacac tagcttcctt gaagccaaac
     8761 gaatcgaatg ggcaagttat gcaaccttcg tttcacaatg ggaaattgat aattatttag
     8821 acctttacta atactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt
     8881 ctaagccatc aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg
     8941 aaaaatcctc ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatca
     9001 aggagtcact atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact
     9061 tcaaatcgaa atctacaagt ttactaactt ttacaagctt caaaattaca catctgtaaa
     9121 tattttttcg aaagatatat ttgaagccat agtaaatt
//
LOCUS       DAVHIU010000077         8863 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVHIU010000077 DAVHIU010000000
VERSION     DAVHIU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.079"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            122..277
                     /locus_tag="VVN06_001496"
                     /pseudo
     CDS             122..277
                     /locus_tag="VVN06_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VVN06_001497"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VVN06_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU5563011.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RFYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDIDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDSFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDIERDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VVN06_001498"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VVN06_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU5563012.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVTFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="VVN06_001499"
     CDS             7614..8792
                     /locus_tag="VVN06_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563013.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2743 a         1854 c         1882 g         2384 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagcaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagtctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagttaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgttttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagaggac ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatatc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgattctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatattga gcgagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggtgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaaattgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggtcgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc ggcagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttaccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactatgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 attgtgtctg tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaagtggtg
     8581 aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaa
//
LOCUS       DAVHIU010000078         8717 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVHIU010000078 DAVHIU010000000
VERSION     DAVHIU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.080"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..135)
                     /locus_tag="VVN06_001500"
     CDS             complement(<1..135)
                     /locus_tag="VVN06_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU5563014.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     N"
     gene            complement(216..2867)
                     /locus_tag="VVN06_001501"
     CDS             complement(216..2867)
                     /locus_tag="VVN06_001501"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5563015.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVVHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(2860..3438)
                     /locus_tag="VVN06_001502"
     CDS             complement(2860..3438)
                     /locus_tag="VVN06_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5563016.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(3438..3998)
                     /locus_tag="VVN06_001503"
     CDS             complement(3438..3998)
                     /locus_tag="VVN06_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563017.1"
                     /translation="MARTELPDKIETECLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYAFLKSEWEVI"
     gene            complement(3985..4434)
                     /locus_tag="VVN06_001504"
     CDS             complement(3985..4434)
                     /locus_tag="VVN06_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU5563018.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(4847..5242)
                     /locus_tag="VVN06_001505"
     CDS             complement(4847..5242)
                     /locus_tag="VVN06_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU5563019.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(5338..5592)
                     /locus_tag="VVN06_001506"
     CDS             complement(5338..5592)
                     /locus_tag="VVN06_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU5563020.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(5602..6936)
                     /locus_tag="VVN06_001507"
     CDS             complement(5602..6936)
                     /locus_tag="VVN06_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU5563021.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(6936..7169)
                     /locus_tag="VVN06_001508"
     CDS             complement(6936..7169)
                     /locus_tag="VVN06_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU5563022.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            7200..7274
                     /locus_tag="VVN06_001509"
                     /pseudo
     CDS             7200..7274
                     /locus_tag="VVN06_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7482..7634)
                     /locus_tag="VVN06_001510"
     CDS             complement(7482..7634)
                     /locus_tag="VVN06_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563023.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
     gene            complement(7631..7813)
                     /locus_tag="VVN06_001511"
     CDS             complement(7631..7813)
                     /locus_tag="VVN06_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563024.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(7815..8594)
                     /locus_tag="VVN06_001512"
     CDS             complement(7815..8594)
                     /locus_tag="VVN06_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563025.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEALWTSREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         2448 a         1997 c         1694 g         2578 t
ORIGIN      
        1 attatttaac ttataagttt taacagctac ggaatcaatt tcaacagcaa cttgaacatc
       61 tatgcctgat tttttaaggc cacttgtagt gccacctgca ccgctaaata aatcaatagc
      121 aacaattttc cccatagtat tctcctaaag tttctccttt ttattataac attatcaaat
      181 gtaaaaccca acccgagagg gttaggtttt taacatcatt tcaccaactt cttcatctca
      241 tcaatacgtg cgacggtcgc gtcatattta gcttggtagt cagcttgttt gtcgcattct
      301 ttttggacga cttctggttt ggcgttggct acgaagcgtt cgttagagag tttcttacca
      361 accatgtcca gttctttttg ccatttagca agttccttgt cgagacgggc cagttcttct
      421 tcaacattga ggagatcggc cagtggcagg tagatttctg ctcctgtgat gacacttgac
      481 atagccagtt caggtgcagg gatggttgat gcgatttcca aatgttctgg atttgtaaag
      541 cgtttgatgt agttgacatt gctgttaaag aaggcttcca agtcgctatc gcttgtctta
      601 acaaggatgg tgataggttt gcttggtgct acatttactt ccgcacgcgc attacgaacg
      661 gcacggatca agtctttgag actttccaca ccagtgtggg cagcaaggtc ctcaaaggct
      721 gggttgacag ttgggtatgc tgctgtcacg atagagcctt cagcgtactg accaaagatt
      781 tcttctgtca cgaatggcat gattgggtga agaagacgga ggattttatc cagcgtatag
      841 aggagaacag atcgagtaat gaccttatcg tcttcattgt cgctgtaaag aacttccttg
      901 gtcaactcaa cataccagtt ggcaaattct tcccaaatga agttgtagag gatgtggcca
      961 gcaacaccaa actcaaactt atcaaagtta gcagttgctt ttccaatcgt ttcattgagg
     1021 ttgtggagaa tccagcggtc cgtcacatta ccagcctcac ctgttgcaac ttttgtgaca
     1081 ttgtcatgca ccacatccag cgtcaaacct tcattgttca taaggatgta gcgagagatg
     1141 ttccagatct tgttaatgaa gttccatgaa gcatccattt tctcataaga gaaacgcacg
     1201 tcttgtcctg gcgcagaacc gtttgaaagg aaccatcgaa gggcatcggc accgtatttc
     1261 tcgataacat ccattgggtc aatcccgtta ccgagagact tagacatctt gcgtccttgc
     1321 tcgtcacgaa taagaccgtg gataaggacg ttttggaatg gttgacggcc tgtgaattcc
     1381 aatgactgga agatcatacg agacacccag aagaagatga tgtcgtaacc tgttaccaag
     1441 gttgaagttg ggaaataacg tttaaagtct tctgagtcga cttcaggcca gcccatggtt
     1501 gaaaatggcc agagggcaga actgaaccaa gtatccaaga cgtcttcgtc ctgagtccat
     1561 ccgtcacctt ctggagcttc ttcgccgaca tacatttcac catcagcatt gtaccaggca
     1621 gggatttggt gaccccacca aagctgacga gagataaccc agtcgtggac attttccatc
     1681 cattgaagga aggtatcgtt gaaacgaggt gggtagaatt cgaccttgtc ctctgtgtct
     1741 tggttggcaa tggcgttctt agccaattgg tccatcttga cgaaccattg agtagacaag
     1801 cgtggctcaa ctacgacacc tgtccgttct gagtgaccga ctgaatggac acgtttttcg
     1861 attttgacaa gggcaccgat ttcttccaac ttagcaacga ctgccttacg agcttcaaaa
     1921 cgatccatgc ctgaaaattc aaaggcaagc tcattcatag ttccgtcgtc gttcatgacg
     1981 ttgacttgtg gcaagttatg acgttggcca accaagaagt catttggatc gtgggcaggt
     2041 gtgattttca cgacaccagt accaaactca ggatctgcgt gctcatctcc aacgattggg
     2101 atgagtttat tagcgattgg aaggatgacg tttttaccaa tcaagtcctt gtagcgcggg
     2161 tcttctggat taaccgcaac cgcaacgtcc ccaaacatag tctcaggacg agttgtagca
     2221 acttcaaggg cgcgtgaacc atcttccagc atgtaattca tgtggtagaa ggcaccttcc
     2281 acatccttgt gaatcacctc aatatcagaa agggctgtgc gagctgctgg gtcccagttg
     2341 atgataaact caccacggta gatccagcct ttcttgtaaa ggtccacaaa gaccttacga
     2401 acagcttttg acaaaccttc gtcaagagtg aaacgctcac gagaatagtc tacagagagc
     2461 cccatcttgc cccattgttc cttgatggta gtggcatatt cgtctttcca ttcccagact
     2521 ttcgtcaaga aagactcacg accaaggtca tagcgggaaa tgccctcacc acgcaagcgc
     2581 tcctcaacct tagcctgagt cgcaatacca gcgtggtcca ttcctggaag ccaaagggta
     2641 tcaaaacctt gcatgcgttt ttgacggatg ataatatctt gcaaagttgt atcccaagcg
     2701 tgaccaaggt gaagtttacc tgtaacgttt ggtggtggaa taacgattga ataaggctta
     2761 gccttttgat cgcctgaagg cttgaaaaca tcagcatcaa gccatttttg ataacgacca
     2821 gcctcaacct cggctggatt gtatttaggt gaaagttctt tagacatgtg tgtgtccttt
     2881 ctctattttg tttattttat tttgaatttg cttagcagct tcttctgcag acaaattcgt
     2941 attatttatt ttaaagtagt ggttcaactc attcggttga tgttgggaat ttaattgaag
     3001 tgtttcagcg gtctctataa tttctctttc agatacctca atatgtcgtt ttaagggttt
     3061 gtactttaat cgattctccg ttcgatttcg acgtatgcac tcttcaagac ttgtttccaa
     3121 ctcaacaaaa agaatctctt gatgataatc atccaacaaa ctctgaattt gctttaaata
     3181 tatcagatga tactgatttg aaaaatcaat tacatctgtt aaaattactg atcgctgatt
     3241 tcttgcactc gctccaagga atgaaaaagt gattccacga acaaattccc acatttcctc
     3301 tgtatagtcc tgatagattt ctagtgcaaa atcaatggct tgatggttat aaaagagggt
     3361 agcatccgtc agtcgagata attcttgacc aatggtcatt tttcctgatg ctggagcacc
     3421 aatgatgaaa agatgcatca aatcacctcc cactcactct tcagaaaggc atatctcaaa
     3481 tcatcacagc agttgccttg agcatctttg cggtctctta tgcgagcttc gagggtaaag
     3541 ccaagttttt ccgcgactcg ttgactttga aggttatatc caaagcaagt tagttcaatc
     3601 ttgtgaagac ccaaatcttt aaaggctaag tcaatcaagg cacgcgcagc ttctggcaca
     3661 taacctcgcc cccaatagtc tgggtgtaag gtatagccga tttccagcac atcatcttca
     3721 tggcgatggt tgaaatcgac agagccaacg atcttatccg ttcctttgac gacaatcccg
     3781 tagcctgctg ggagattttc cttttgatta cgctccggaa gaatgtgctc cagataataa
     3841 atctcatctt ccaaggtctt gactggagga aaacctgctg gataggcgac ctctggcaaa
     3901 ctagcatagt caaagatatc ctcagcatcc gccacagtac ggactcgtaa aacgagacat
     3961 tctgtttcga ttttatctgg tagctcagtt cttgccatcc ttcttcctcg cttttttgat
     4021 gaaactgccc ttcatatcta cacgcttgtc cagatagcga taaacgcgct gatatccatc
     4081 tcccatgaaa taggttgggg caaacagttg atttttaaaa tgtccctttt catccaggag
     4141 ttctggaaca acaagtcgct caagaatctt ggcaaagatg tggcaaatac cgtcttcctc
     4201 aacaatccta tctacccgac aatctaaaac aagtggacag gcgtctaaaa taggagtctg
     4261 agttcgttca gaaatttcat aatgcactcc caaacgttcc aatttctcct gatgactgat
     4321 aaaaccagcc tgctccatcg caagcataga agtttcatca gaaatattca cagtaaattt
     4381 ttgatactgt ttgatctgct ctgcggcatt ctctctcgca acgactccaa tcacaaccca
     4441 atctcctaga ctataagagg aactacaggt cgtgatgtta tagccaaaat tctaatcttg
     4501 atatcctaaa ataaaaacag gaaaaccata atatagttta cttgtgttaa aagattgctt
     4561 cataacaacc ccctttgact aagacgtaaa agaaaagccc tgccatctac atgacaggga
     4621 cgaatgtgtt tatccgcggt accacccaat ttcgggcagt tgcccgcaac tctcgtcttt
     4681 aaagcaaaaa gacactttta tttcaatttt tcatccatta gcaaccagtt ttgacacatt
     4741 tgtggacttc tcagcgccgc cactttctgt aaaatgtggg attcaaaaca cctctaatgg
     4801 ctctattgta gcaagatttt ttcggaaatg caaggctcaa gaaaaattac ttgaacttga
     4861 tgccattttc tttcaatttc ttgatggtag ctgggccgat tcctttcaag gcaaggagtt
     4921 ctttttcagt ccagtttttg aaatctgaag cagacttgat tccttcatca tagaaagtct
     4981 tggcacgatc aagtggaagt ccacccaaat ttgctgcaaa atcttctaca gaattagcta
     5041 ctttttgagc ttgctctttg gtagcttcta ctgcttgttc aactttttta gaaacagcct
     5101 taccagctgc gcttgctgac tgttctgcac gcttcacgac tttttttgtc tcatctacaa
     5161 ccttagtcac agtacttgaa aaggcacctg cgcgacggag gccatttcgt aattgttttt
     5221 tacgattgag tttctttgac atgataaaat cctttcaata aaaaatccct gctctgacaa
     5281 ggatttaaca taaacattct atcaagattt tagtgaaaaa tcaagaatct ttctcgttta
     5341 ataatttcgc tcaccaaaca agccttctgg caaatcatgg aatcccttgc ctgctttgaa
     5401 gttttcaaac ttaccaagca agaactgata agcatccaag cgactctcgt agcgaatcat
     5461 ggcatctttg gcacgctcgt cctggtcttc ttcgtaaact ttttgacttt ccttgtgcgc
     5521 catatcgtta atcatgattt gggtattgac ccaagcttca aattccttca taaattcttg
     5581 ttcgtaactc attttttctc cttattctaa tccacgaaaa tagtccgtat caatctcacg
     5641 gtagggctgg atatcctgaa catactccaa cactccttgg aattctccgt cttcatcgtg
     5701 tactgcagca taggtgatgt ggacaaactt acctcgcgac tcagacttga accacatttc
     5761 atacttgtct ttgcttccct cacgaagccc cttcatgata gttttgacct tgtccaagta
     5821 cttaggcgga tggcagagtt cgacattgcg cccgacttgg gacggcgtcc gtttgaaaat
     5881 catctcatca gctggcgtat tgtcattgta atactggaaa atatcttctt tattgacaaa
     5941 ggtaatctcc atagggagat gattgaggat gagattggcc tgctcgactg aaagatagcc
     6001 attaccaaaa gcctgttgac tatggcggtc cagcacagct tccttttcct taggggtaaa
     6061 ggtaatggta aaatggcctt ctggcgtatc tatgacttgt tgaacttgac cttctgccgt
     6121 atctagctgt acaggttcct ctgcaatctt ttcctcaata aagctctgtc gttctggcac
     6181 ccatttctct gacggacgga tgatggcata gccataggca tcgctctcct ccgcaatctg
     6241 aagccagtca tcctgagtaa aagactcaag gagaatcatg aggaggatgg actcttcctt
     6301 gaaaatcata ctttcaaact ctgtcgcaaa agcttcaaaa gcttccttta cactgctaat
     6361 tgacacttct ggtagtgact tggctgtcgt tagagctgtt tgaaagagtt ccctaatctg
     6421 atcatccact ccccacataa ctttgggagg tgaatcgtgt ccatagcgct ccatgatagg
     6481 aaagaagagt tcttccttac gttggtaatg gatgtcaaat tgccccacaa gccccatctg
     6541 acgcaccaag cccttgcgca tctccgcaag catttcctcg tcttccatca actcataggt
     6601 atctaacaat ctacgaatgc gaatcaaagc tgcacggaga gccagatttt cttctttgaa
     6661 gacacgaact gggtgacctg gatgctcagt atctgaaact tcgacacctt tgatagcatt
     6721 tttaaaaaga ttggcatgga catcacagag ttccataaca tcttcaaaag tgacgcccga
     6781 atccgagttc atcagctcgt gctccataag ggaaatctcg atggctgaca cgcccgtaaa
     6841 ggtcgcatca aagcgatctt gaaccgactc aggagaggcg ccattgtgca attctaacaa
     6901 aatatcccgt aggacatgaa tccgttcatc tgccattagt ctaatccaat cacttcgtag
     6961 ccattcgctt ccagtgtgcg tacaatcttg tccataggag ttcctgctag cttagaaccc
     7021 tgtttaagtg atactttacg accaactgta ttgcgcatta agggattggc aaggggttta
     7081 aaacccaact ccactagaat ttccaagact tctggatgct tgtccaccac ttctgcaaca
     7141 ggaattgaca catcgatgat attgtccatg acgacctcca ttgtatgttc taatactcaa
     7201 tgaaaatcaa agagcaaact aggaagctag ccgcaggttg ctcaaaacag tgctttgagg
     7261 ttgtagatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg gatagaactg
     7321 acgaagtcag taacatatat actgcaaggt gaagctgacg tggtttgaag agattttcga
     7381 agagtataaa atgattttac tgcttatatt ataccatatg gaaagagaat aaaaaactag
     7441 cagtggagtt cctgctagtt ctctgcgact tggattacca tctagataag tttaaagagc
     7501 caatccaata tcttaaacac tatcttgtaa aacagcaatt ggactgcaaa agagatgatc
     7561 atccaaaaga atttcacaat cccaataatc ggtttgataa aaataatggc aagaagtccc
     7621 cagaaaaatt tcatttttta tcctctggct taatgagcca ccgagccgta tccattagct
     7681 tatctgcaat aaagagtttt cccagaccaa caacgacatg gtcatctttc tttatcgttt
     7741 tcattacttt tacaccttgc acggtcaaaa agtaattacc ataaatttta gctagagttt
     7801 ttatgagtcc catattactc tccttcgatg atttcagcct cttttcggat tgtttcaaca
     7861 tcttcttgat attgaacttc actatagtta actagcttgg acaattcttt ctgcaagctt
     7921 tcacccatcg gtttcatagt tgcaaaggtt aaaccacctg aaatgatacc acccaagata
     7981 ggaataaatt tccccattcc tttggccagc cctcccttgg taagattcac accaaatatt
     8041 cttaagactt ttttcaaaat aggataccaa agcgtctttg ttaaagcttt attaggtact
     8101 gttttcatta cctgtttggc aattgttata ccaccagcac gtagcaaagc agcagttcca
     8161 ttcaccccca acatgacgcc tagataaagc aagagggtat tttgagcatc ttcacttaac
     8221 tcctcacgtg aagtccaaag agcctcataa ccataaatat aacctaattc ttgagccaat
     8281 ttcagagaga aagcataaaa ttgagccaca tcagctggaa tggtaattgc catagcaagc
     8341 cctccaggta atcctgccaa aacagaagtc ccactcgcta ataatacatt gtcccgaata
     8401 caagcattag ctacacgatc taatatttct tgagatagaa gagtcgttgg accttgttcc
     8461 aataaggtag gaatatcttt tggatccaat tctttggaaa acttatccac taaaaatttc
     8521 gaacgatcaa ccttaacaac aggtagtttc accacttgtt gcaatacttg catagctaag
     8581 tcttgttcag ccatatacaa actccttatt tcttttttgc accttatcat attatatttc
     8641 ctacaaaaaa agcattttct aattagataa cattaaaaaa tacagaaccc cttttttata
     8701 ttaaaatgaa acaagaa
//
LOCUS       DAVHIU010000079         8544 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVHIU010000079 DAVHIU010000000
VERSION     DAVHIU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.081"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..777
                     /locus_tag="VVN06_001513"
     CDS             <1..777
                     /locus_tag="VVN06_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5563026.1"
                     /translation="IDSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIIS
                     STGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMK
                     AVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAM
                     QHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALST
                     IAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1379..1459
                     /locus_tag="VVN06_001514"
                     /pseudo
     CDS             1379..1459
                     /locus_tag="VVN06_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2098..3459
                     /gene="trpE"
                     /locus_tag="VVN06_001515"
     CDS             2098..3459
                     /gene="trpE"
                     /locus_tag="VVN06_001515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU5563027.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPIFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAVGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3456..4022
                     /locus_tag="VVN06_001516"
     CDS             3456..4022
                     /locus_tag="VVN06_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5563028.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            4033..5037
                     /gene="trpD"
                     /locus_tag="VVN06_001517"
     CDS             4033..5037
                     /gene="trpD"
                     /locus_tag="VVN06_001517"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5563029.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5034..5801
                     /gene="trpC"
                     /locus_tag="VVN06_001518"
     CDS             5034..5801
                     /gene="trpC"
                     /locus_tag="VVN06_001518"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU5563030.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5788..6387
                     /locus_tag="VVN06_001519"
     CDS             5788..6387
                     /locus_tag="VVN06_001519"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5563031.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6365..7588
                     /gene="trpB"
                     /locus_tag="VVN06_001520"
     CDS             6365..7588
                     /gene="trpB"
                     /locus_tag="VVN06_001520"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5563032.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7581..8357
                     /locus_tag="VVN06_001521"
     CDS             7581..8357
                     /locus_tag="VVN06_001521"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5563033.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8329..8430)
                     /locus_tag="VVN06_001522"
                     /pseudo
     CDS             complement(8329..8430)
                     /locus_tag="VVN06_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2505 a         1608 c         2067 g         2364 t
ORIGIN      
        1 atcgactctt ttcctttaat ttggtttcat acgctgttag ataaagtgtt acggacgtgt
       61 aaagaatttg gtgttaatgc tatagttgag tactttggag aggaggatac aattagtaat
      121 tcaattatat caagcacagg tagcctagta gatggtgtta ttgtttttta tgaaagtgta
      181 gatgatatca gaattcagta tttaaagaag aaccatatgc cttttcttgt tttcggtgaa
      241 tctcaaacgt ctggagtagt ctatgtatct aataataatt ttcaagctac ttatgatatg
      301 atgaaagctg taactgaaga aaaatttaaa aatatgtggt tgcttatggg aggagaatcc
      361 catgttaata aggatcgtga aagaggtgtg cgttcttttc tgaatgataa aaattacttt
      421 atggatttaa aagtcattta tggcttatct acaattgatt cagtttattc ttatgcaatg
      481 cagcacttga ctacccaaaa ttatccggac attatttttg tttcaggaga tgaaaaggtt
      541 cagggcttga ttcgtgcgtg ctatgagaaa ggaattctta ttccagatga tatttctatc
      601 attggatttg ataatattcc tatttctcag tattatactc cagctttgtc tacaattgct
      661 cccaattatg ttaaattggc taaagaaatg atagaaggtg ttttagcaat tattaaaggg
      721 gaaagtgtca catctgttga agtttctcct aaatttgtta ggagacagag tttctagtat
      781 tgtttaattc ttgaaaatcc tagtggaaaa gatggagtgt tctaaaataa gttctgttta
      841 gcctccattc agtttctatt gagttggatc caatttctaa caagtgggac atgttcatgt
      901 tcgatctaag gaagacatgg tcacattcga atgatatttt caaaaaaatc gtaaaaaagt
      961 cttgacaaag aaaaaacaaa gtattaaaat aagttcaaca aatcagaaaa gtaactattt
     1021 ttgatcttca gggagcctgt ggtgattgtg aataggtggt tggaagtagt gaaagtgggc
     1081 tgattttgaa aatgaatttg aaacaatgaa aattcggtgc gcacacctta cagtgcagct
     1141 tgttgttaaa caaggcagag atgtaaaggg atagtccctt tataattgag gtggcaccgc
     1201 gttaccaacg ccctcacacg gaagtatatt ctgtgtgtgg gcttttttct atccgtcgtt
     1261 tggtttatct tttattaggg ccttaagtcg ctttgatgaa cttgagttct atctacagct
     1321 ccttgcctac tactaaaagc aaacaaaaag gctggactaa tacgaaaaaa gctagtgata
     1381 ctcaatgaaa atcaaagaac aaactaggaa actagccgca ggttgctcaa aacactgttt
     1441 tgaggttgtg gatagaactg acgaagtcag ctcaaaatac tgttttgagg ttgtggatag
     1501 aactgacgaa gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag
     1561 ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagctcaa aacactgttt
     1621 tgaggttgca gatagaactg acgaagtcag taaccatatc tacggcaagg cgacgttgac
     1681 gcggtttgaa gagtttttcg aagagtataa aggtgtaccg agatgaacat ctcttcatca
     1741 atgtctctgg tcgtggaggc aaggacctag ctgcgattgc agactactag aagctaaaaa
     1801 ataatagatg gaaaaattac agttctttct ctcacagaga gcttgtggtt gctgaaaaca
     1861 agcagagaaa gctgtaaagt tgggttcatc tgaaacgaga gaagaaaaca tcattctcaa
     1921 gggagtgccc tttatcgcac agcctgttac aggagatatc tgagacagga atgagagata
     1981 ggagaaatcc tataattgag gtggcaccgc gaatttcgtc ctcacgcaag ttattttgcg
     2041 tggggatttt tcatacagtc gctacgaact agaattagaa ctgaaaggaa aattacaatg
     2101 gaacgaatca ttcatggaga tgtcttatca ccaatcttgg cttatatgcg cttaaagggg
     2161 caacacaagg ttatcttaga gagtattccg agagacaagg aaacagctcg tttttctatc
     2221 ctagcctata atccaatttt tgagattaag tttgaaaatg gagttctcta tcaaaatggt
     2281 caagtgattg atcgggatcc cttggatttc ctttatgaag tgattcataa gagtcagcac
     2341 cactcagagc taccttttgg tggtggagcc gttggttttg tgggttacga tatgatttca
     2401 ctttatgaag aaattggtca aatccctgag gatacaattg ggacgccaga catgcatttc
     2461 tttgtctatg agagctatat ggtctttgac cacaagaagg agaagataca tgtcatagag
     2521 gatgcacttt atagtgagcg cagtcaagag gccttggaaa aatccttgaa ccaagtgctt
     2581 gaggaattac gcattcctgc tccaaatgaa tttgaagact tggatctatc tccgttagac
     2641 ttcaaacctc atatcgctcc tcagaagttt gaggaaatgg tggaaacagc tcgtgacttg
     2701 attcgtaatg gtgatatgtt ccaatgcgta ctcagccagc gcttttcagc agaggttact
     2761 ggaaatccat ttgacttcta cagaaatctc cgcgtgacca atccatctaa ttacctttat
     2821 ttctatgact ttggggatta tcaaatcatc ggtgccagtc cagaaagttt ggtttctgtc
     2881 aaaaatggca tcgtgacaac caatccgatt gcagggacgc gaccaagagg ggctacggat
     2941 gaagaagaca agaccttggc gactgacctc ctctctgatg agaaggaaac agcagaacat
     3001 cgaatgttgg tagacttggg gcgtaacgat attggccgca tctctgaaac ggccagtgtc
     3061 caagttacta agtatatgga ggtggagctc ttccgctacg tcatgcattt gaccagtgtg
     3121 gtcaagggac gtttgcttgc agaactcact gccatggatg ccttgaaagc aacgcttcct
     3181 gctggaaccg tttctggagc accaaagatt cgggcgatga gacgcatcta tgaactggaa
     3241 acggaaaaac ggggcgtata tgcaggagca atcggctact tgtctgcgac gggtgatatg
     3301 gatttggcca tcgccatccg aaccatgatt ctcaaaaatc aaagagccta tgtgcaggct
     3361 ggggcaggga ttgtctacga ctctatcgcc caaaacgaat accaagaaac cattaacaaa
     3421 gcaaaatcta tgactagaat tggagaacta agaccatgat tttattgatt gacaactatg
     3481 attcttttac ctataacttg gcccaataca ttgggaattt tgcagaagtt caggttctga
     3541 gaaatgatga ttccaagctg tatgaagaag ctgaaaaagc agatggtctg gtcttttctc
     3601 ctggtcctgg ttggccagtt gatgctggaa agatggaaga catgatttgt gattttgcag
     3661 gcaagaagcc gattctaggg atttgtttgg gtcaccaagc cattgcagaa gtctttggtg
     3721 gtaagctagg tttggctcca aaagtcatgc atgggaaaca gagcaatatc aactttgaag
     3781 cgccatctgt tttgtatcaa ggtattgagg atggccgtgc ggtcatgcgt tatcacagta
     3841 ttttgattga ggaaatgcca gaagactttg aagtgacagc tcgttcgact gatgaccaag
     3901 ccatcatggg gattcaacat aaaaacctac caatttatgg cttccagtac catccagaga
     3961 gcattggaac gccagacggc ttgtcttcta ttcggaattt tatcgaggag gttgtaaagt
     4021 gaggaaacta ggatgaaaga gattattgaa aaactagcaa aatttgaaaa tttatcaggt
     4081 gtggaaatga cggatgtcat tgagcgtatc gtaactgggc gtgtaactga agcgcagatt
     4141 gcttctctcc tcttagctct taagatgaag ggggaaacac ctgaagaacg tacagccatt
     4201 gcccaagtca tgagaggaca tgcccagcat attccaactg agattcatga tgctatggac
     4261 aactgtggta caggtgggga caagtctttc agttttaata tttccacaac tgcagccttt
     4321 gtcttggctg gtggcggtat tcacatggcc aagcatggta accgctcgat ttcttctaaa
     4381 tctggttccg cagatgtcct cgaagccttg ggaatcaatc ttgacctcaa accagctgaa
     4441 ctaggtaagg tctttgataa aactggaatc gtctttctct tcgctaaaaa tatgcaccca
     4501 gctatgaaat acatcatgcc agctcgtttg gaattgggaa ttccaacgat tatgaacttg
     4561 actggtccac tgattcatcc aatggcttta gaaacacagc ttcttggaat tagtcgtcca
     4621 gaactcctag aaagtacagc tcaggttttg aaaaatatgg gtcgcaaacg tgccatcgtg
     4681 gttgctggac cagaagggtt ggatgaagct ggcttgaacg gaacaaccaa gattgcactt
     4741 cttgaaaatg gcgaaatcag cttgtcaagc tttactccag aggatttggg aatggaagac
     4801 tatgctatgg aagatattcg tggtgggaat gctcaggaaa atgcagaaat tttgcttagc
     4861 gttctgaaaa acgaagcaag tccattcttg gaaacgacag tcttgaatgc tggtcttggt
     4921 ttctatgcta atggtaagat tgatagcatc aaggaaggag ttgccttggc ccgtcaagtg
     4981 attgctagag gcaaggccct tgaaaaactc agactgttac aggagtacca aaaatgagtc
     5041 aggaattttt agcacgaatc ttagagcaga aggcgcgtga ggtggagcag atgaagctgg
     5101 agcaaatcca gcctctgcgc cagacctatc gcttggcaga atttttgaag aatcatcagg
     5161 actgcttgca ggtaatcgct gaggtcaaga aagctagccc tagtttggga gatatcaatc
     5221 tcgatgtgga tattgtgcaa caggcccaga cttatgaaga aaacggagca gtgatgattt
     5281 cggtgttgac agatgaggtt ttctttaaag ggcatttgga ttatctacgg gaaatttcca
     5341 gtcaggtaga gattccgacg ctcaacaaag actttatcat agatgaaaag caaatcatcc
     5401 gcgctcgcaa tgcaggtgcg acagttatct tgcttattgt ggcagccttg tccgaagaac
     5461 gcctcaagga actgtatgac tacgcgacag agcttggtct ggaagtctta gtggagactc
     5521 acaatctagc tgaactagag gtagcccaca gacttggtgc tgagattatc ggggtcaaca
     5581 accgcaactt gactaccttt gaggtcgact tgcagaccag tgtagattta gccccttact
     5641 ttgaggaagg tcgctattac atttctgaat ctgccatttt cacagggcag gatgcggaac
     5701 gactagcccc atactttaac ggaattttgg tagggacagc tcttatgcag gcagaaaatg
     5761 tggcccagag aatcaaggag ttgcagattg acaaaggtta aaatttgtgg actatcgacc
     5821 aaagaagcgg tggaaacagc cgtttcagca ggagccgact atatcggttt tgtctttgca
     5881 cctagtaaaa gacaggtgac tttagaagag gcagctgagt tggcaaagct tattcctgca
     5941 gatgtgaaaa aggttggagt atttgtttca ccaagtcggg tagaactgct ggaagcgatt
     6001 gacaaagttg gcttggactt ggttcaagtt cacggtcagg tggcagctga tttatttgag
     6061 aatttgcctt gtgccagtat tcaggctgtg caggtagatg gaaatgggca tgtccccaat
     6121 tctcaggcag attatctact ctttgatgcc cctgtggcag gaagtggtca gtcctttgat
     6181 tggggtcaac tggatacgac tggactagca cagcccttct ttatcgcagg tggccttaat
     6241 gaagataatg tagtaaaagc aattcaacat tttactccct atgcagtaga tgtatcgagc
     6301 ggagtagaga cagatggaca aaaagatcat gaaaagatta gaagatttat agagagggta
     6361 aagcatggca tatcaggaac caaataaaga tggattttac ggaaaattcg gcggacgttt
     6421 tgtcccagaa acattgatga cagcagtttt ggagttggag aaggcctacc gtgaaagtca
     6481 ggcagaccca agtttccaag aggaattaaa ccagcttttg cgccagtacg taggacgtga
     6541 aactcctctt tattacgcaa aaaacttgac ccagcatatc ggcggagcca agatttatct
     6601 caaacgggaa gaccttaacc atacaggagc ccacaagatt aacaatgcct tgggacaagt
     6661 ttggcttgcc aaacgcatgg gtaaaaagaa aattatcgca gaaactggtg ctggtcagca
     6721 cggtgtggca actgcaactg ctgcggccct ctttaacatg gaatgtacca tctacatggg
     6781 tgaggaagat gtcaaacgcc aagccctcaa tgtcttccgt atggagcttt tgggagctaa
     6841 ggtcgaggcc gtgacagatg gttcgcgcgt gctcaaggat gcggtcaatg cagcccttcg
     6901 ttcatgggtg gctaatatag atgataccca ctatatcctt ggttctgcct tggggcctca
     6961 tccatttcca gaaattgttc gtgacttcca aagtgtcatt ggtcgagagg ctaaacaaca
     7021 gtaccgtgac ttgacaggac aaaatctgcc agatgcccta gtagcctgtg ttggtggtgg
     7081 gtcgaatgcc atcggtctct tccatccctt tgtagaagat gagtcagtag ccatgtatgg
     7141 agctgaagcg gctggatttg gtgtggatac ggagcaccac gcagctacct tgaccaaggg
     7201 tcgtccaggt gtccttcacg gttccctcat ggatgtgctc caagatgccc atggtcaaat
     7261 tcttgaagcc ttctctatct cagcaggttt ggactatcct ggtatcggtc cagagcattc
     7321 tcactaccac gatattaaac gtgccagcta tgtccctgtg acagacgaag aagccttgga
     7381 aggattccaa ctcttgtctc gtgtggaagg gattatccca gctttggaat ctagccatgc
     7441 tatcgccttt gcggtgaaat tggccaaaga acttggacca gaaaagtcta tgattgtatg
     7501 cctatcaggt cgtggggaca aggatgtggt tcaagtaaaa gaccgcttgg aagcagatgc
     7561 agcaaagaag ggagaagctc atgcctaaga cactaacaaa aaaattaaat gctattaaag
     7621 cagctggaaa aggaattttt gttccctata tcatggctgg tgaccacgaa aaaggtttgg
     7681 atggtcttgc tgaaacaatt cactttttag aagatttggg tgtctctgca attgaagtgg
     7741 gtattccctt ttcagaccct gttgcagatg gacctgttat cgaagaagct ggcttgcgca
     7801 gtttagccca cgggacctct acccaggctt tagttgaaac cttgaaaacc attgaaacag
     7861 agattccact ggtcatcatg acctacttca accccctctt tcagtacggt gtggagaact
     7921 ttgtcaaaga tctggctgat acagctgtga agggcttgat tatcccagac ctgcctcatg
     7981 aacatgccaa ctttgtagaa ccatttttgg cagatacaga tatcgccttg attcctttag
     8041 taagcttgac cacaggaatt gagcgccaga aagagttgat tgaaggggcg gagggattcg
     8101 tctatgccgt tgccatcaat ggggtaacag ggaaatcagg caattaccgt gcagatttgg
     8161 acaagcactt ggcgcaactt catcaagtgg ccgacatccc agtcttgaca ggttttggtg
     8221 tatctagtca agccgatctg gaacgcttca atgcggtgtc agatggtgtt atcgtcggtt
     8281 cgaaaatagt aaaagctctc caccaaggag agccgattca ggactttatc aaacaagcag
     8341 tagcttacca aaaataatta aacaagtagc aagtaagagg aaaggcacga aaggaagtct
     8401 ttcctttttc ttttgcagga gaaaggctag ttttatagtg aattgaatct ggaatattac
     8461 actacggttc ctaaaatatt ttcagaaagt gatttgaatt tcccaataaa tttgttcagt
     8521 ttttaattga ttttactata gaag
//
LOCUS       DAVHIU010000080         8231 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVHIU010000080 DAVHIU010000000
VERSION     DAVHIU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.082"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..694)
                     /locus_tag="VVN06_001523"
     CDS             complement(<1..694)
                     /locus_tag="VVN06_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5563034.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEK"
     gene            complement(750..1772)
                     /locus_tag="VVN06_001524"
     CDS             complement(750..1772)
                     /locus_tag="VVN06_001524"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU5563035.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(1904..4375)
                     /gene="parC"
                     /locus_tag="VVN06_001525"
     CDS             complement(1904..4375)
                     /gene="parC"
                     /locus_tag="VVN06_001525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU5563036.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(4795..6738)
                     /gene="parE"
                     /locus_tag="VVN06_001526"
     CDS             complement(4795..6738)
                     /gene="parE"
                     /locus_tag="VVN06_001526"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU5563037.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            6874..7515
                     /gene="plsY"
                     /locus_tag="VVN06_001527"
     CDS             6874..7515
                     /gene="plsY"
                     /locus_tag="VVN06_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5563038.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIMFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVVFGFAPVFCLYLAVVFFGTLYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILNNYDPLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(7560..>8231)
                     /locus_tag="VVN06_001528"
     CDS             complement(7560..>8231)
                     /locus_tag="VVN06_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5563039.1"
                     /translation="RVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDV
                     GKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCS
                     PNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPD
                     NGMMESFFGILKSEMFYGYEKSFQSFKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYR
                     TKSFG"
BASE COUNT         2235 a         1867 c         1551 g         2578 t
ORIGIN      
        1 atttttctag ttctttttgg tatttgctat tggattccaa tttttctttt tgccattttt
       61 taaaaacctc gttatattct tttgttgtaa caatatcttt ttgcaatttc attcctttaa
      121 agatatatgg ttccccctta ataccaactt gtgagtatgg ttttgagaat ggtactacgt
      181 tacttacaac tggaaaacca ccagatgaag ctgttggcat caataatgaa ctatctgtcg
      241 accaagcttg agctttggca tatttttcat atctcttctc taggtcagtg gtctcagaaa
      301 cagcatcttc taacaatttc ttatatttat ccaaaccagg tttagctaca acatccttat
      361 cttttccttt cgtaatgcca aggtgtttca tggcagaacc agattttgga tccataatat
      421 tcaagtaaga cgctggatct tgatagcttg gagcccatcc tgtactgttc aaatcatagt
      481 ctttttgaga aggagcaaca ttgccgtatt tatcattttc catcaaacca tcaataacat
      541 ttccaataac gtctgtcctc gatgttcgag tcgctatact gtagcccaat gatgctggat
      601 ctactgcata gacataagaa aatgttgtcg gtgcatctgc ttctttatca gtttttccac
      661 aagccactaa aatagctgac gtgctcagga ccactcctgc tgttaagagc cactttttct
      721 atttcataaa aaatctcctt tggtttattt taatctactt ttacaatcca accttctggc
      781 gcttcaatat cgccaaactg aatacccgtc aattcattat ataatttacg cgtcacagga
      841 cctacttctg tttcactata gaatacatgg aaatcatcac catgttgaat acctccaatt
      901 ggagaaataa ccgctgctgt accacaggca cctgcctcta caaaacggtc aagattatca
      961 attggaacat caccctcaat aggagttaat cccaagcgat gttctgccaa ataaagcaag
     1021 gaatacttgg taatagatgg caagatagat ggactcaatg gtgttacaaa ttcattatca
     1081 gctgtaattc caaagaagtt agctgatccg acttcttcaa tctttgtatg agttgatggg
     1141 tccagataga taacatctga gaaatggcgt gacttggcca tttttcctgg taagagactt
     1201 gcagcatagt ttccaccaac cttagccgca cctgtaccat ttggtgctgc acggtcgtac
     1261 tcatcctgaa tcaagaaatt ggttgggacc aaaccacctt taaagtaatt tccaactggc
     1321 atagcaaaga tggtgaaaat gtactcttct gccggtttta ccccgataat atctccgaca
     1381 ccaatcaaaa gagggcgaag atataaggtt ccacctattc cgtatggtgg tacgtattct
     1441 tcattcgcac ggacaactgc tttacaagct tctacaaaca tgtctgtcgg aacttgtggc
     1501 atcaagagac ggtcacatgt acgttgcagg cgtttagcat tttcatcagg acggaacagt
     1561 tgaacactgc catccttagt acgataagct ttcaaacctt caaatgcttg ttgtccatag
     1621 tgaagacttg gagaagactc tgaaatatgc aaagttgcat cctctgtaag ctctccttga
     1681 tcccattgtc catttttgaa atgagcaaga tagcgataag gtaatttcat ataggaaaaa
     1741 ccgaggtttt cccaatcaat cgttactgtc atattccact ccttattcta aaaacctatt
     1801 tcttatattc tacactattt ttctaaaata gcaagtatat tttgtaattt tcagaaaatt
     1861 tctccaataa aaaccagctc ttagaactga ttcttcattt cacttattta tcttcagtaa
     1921 ctacttcctg aagataagcg tcaaaaactt cttcatctga aatcgtgtca gaaatgaagc
     1981 ttccattgct agtgcgttct gacaagttca agtcttgcaa tcggctttca tagattgttc
     2041 ctttattgga ttggacaagc agagtttggt cgttcacatc cacttccgta ctgaagaaat
     2101 cgccaacaaa tccttgctct gcaactgctc ctgccaagaa gacacgatgc ggtttgtttt
     2161 tcaactcacg caagacttgt aatcctcgtt tggcacggct ggttgctaga atttcctcaa
     2221 tggaaacacg tttcaagctt ccacgctggg tcaagaggta gaaggacgaa gtattacaga
     2281 taaagccaga ttggaggaca tcatcttctt tcaaattcat agccttgaca cctgctgcct
     2341 tagcaccgac aaccggaacc tcttcgatat tgaaacgcag ggcataacca ttttgactaa
     2401 ccaagacaac atcatctagt ttaatcggag ccactgctac aatctgatct gtatcgtctt
     2461 tgagcttagc atacttgaca gacttagatc tataggtccg ccatggagtg aattcttttc
     2521 gctctacccg tttgatttga ccaaggcgag tcactgcaaa gtaggttgtc gcatcgtcaa
     2581 actgatccag tacttccaca taaaggattt cttcattcgt ttcaaagttt gtgatggttt
     2641 ggctcagatg ctctccgatg tccttccaac gaatatctgc caactcatgg attggtctgt
     2701 agatgacatt tccaagactt gtgaacatca agaggtgctg ggttgtcttg gcagattgaa
     2761 caaaaatcaa acggtcatca tcacgcttgc caatttcttc caaggtggaa gccgcaaagg
     2821 aacgtggact ggtacgcttg atgtaacctg ccttggtcac gctgacgtag gtatcttcct
     2881 cagcgataag actagctgta tcaatctcaa ttgctttcgc agtgtcttct aaagaactca
     2941 aacgaggagt tgcaaatttc ttcttgacct cacgaagttc tttcttcatg agattgtaca
     3001 tagtcctttc atcaccgata atagccgcca gcatagcaat cttctcacga agctctgctt
     3061 cttcttcctg caagacaacc acatcggtat tggtcaaacg gtacagttgc aaagttacga
     3121 tagcctcagc ctgttcttcc gtaaaatcat agctaacttt gaggttttcc ttggcgtccg
     3181 ccttattctc agaagcacgg ataagagcaa tgacttcatc caaaatcgaa atcacacgaa
     3241 tcaaaccttc gacgatatgg agacgtttct cagccttttc tttgtcaaag cgtgaacgcg
     3301 ccaaaatcac ttctcgacgg tgagcgatgt agctagacag gattggaaca atcccaacct
     3361 gacgaggtgt gaaattgtca atcgccacca tattaaagtt gtagttgatt tgtaggtcgg
     3421 tgtacttaaa taagtagttg agaacaagct cagtattagc gtctttctta agttcgatag
     3481 cgatacgaag accatcacgg tcagactcat cacgaacctc agcaatccca gctaccttgt
     3541 tattaacacg aacatcatcg attttcttga ctagattggc cttattgatt tcataaggaa
     3601 tctcagtaat aacgatttgt tccttaccac cttttagctt ttcaatttca gtcttggaac
     3661 gaacaaccac gcgccctttc ccagtctcat aagctttctt gatttcatca cgaccctgaa
     3721 taatagcccc tgtagggaag tctggtccag gcaagaattc catgagttta tcaatctttg
     3781 cagttgggtg gtcaatcatg taaactgcag catctatgac ctcagctaaa ttatggggag
     3841 gaatgtctgt ggcataacca gccgaaatcc cagtcgaacc attgaccaag aggtttggaa
     3901 aggctgctgg caagaccgtt ggttctttct ccgtatcgtc aaagttccat gcaaaaggaa
     3961 ctgtcttttt ctcgatatcc tgaagaaggt agcctgcaat ttcagacaaa cgtgcctcag
     4021 tataacgcat agccgcagga ggatctccgt ccatagaacc gttattaccg tgcatttcaa
     4081 ctagaatctc acgatttttc cagttctgtg acatacgaac catggcatca tagatagaag
     4141 aatccccgtg tgggtggaaa ttccccatga tgttcccgac tgacttggcc gacttacggt
     4201 agctcttgtc aaaagtattg ctatccttat tcatagaata aagaatacgg cgctgaaccg
     4261 gcttcaaccc atcacgaata tctggcaaag cccggtcttg aataatgtac ttggagtagc
     4321 gaccaaagcg ctctcccatg atgtcctcca gggacatgtt ttgaatgtta gacataagat
     4381 acaaagccca taaaatacca agtgaaaata gaaaattctt gaagtaagca aactcacaag
     4441 agaatttatc tttttcacac agtatctagg gcgtgttcaa ctcctttcaa agaatgtaga
     4501 gtaggttttt atgcagtaaa agatatttta cgggaattcg tcccgtgttc agttacgata
     4561 agtaaccaaa ctatcctgtt tgtatttttc aatatgaaaa tatggttttc caaaattagt
     4621 cttagtttgt gtcttagccg ctcccttaaa cgcctctttg agataagcac tcatagcaga
     4681 ttcttcatta ataatcctgc aattttttca aaccaagatt ttcaaactgc tttttcacat
     4741 agtcattcac atccgactct aatttccagt ttactaacat attattttct ttcattaaaa
     4801 cactgtcgct tcttctagcg taaacttgac attatcttca atccatttac ggcgtggttc
     4861 taccttatct cccatgagaa cattgacgcg gcgttcggcg cgcgctaaat cttcaattgt
     4921 gacacggatg agggtacgtg tttctgggtt catggttgtt tcccagagct ggtccgcatt
     4981 catctcacca agtcctttgt atcgttggag ggtagcgcct ttaccgaact gtttacggag
     5041 ttcttctagt tctccgtccg tccaagcgta ggccacttct tctttcttgc ctttaccttt
     5101 ggacatcttg taaagaggtg ggagggcaat atagacatga cctgcctcga ctagcggacg
     5161 catgtaacgg tagaaaaatg tcaagagcaa ggtctggata tgggcaccgt cggtatccgc
     5221 atcggtcatg ataatgatct tatcatagtt ggcatcttca atagagaagt ctgctccaac
     5281 acccgcacca atggtataaa tcatggtatt gatctcttca tttttgagga tatccgccat
     5341 cttggccttg gctgtattga caaccttacc acgaagaggt agaatagcct ggaacttgcg
     5401 gtcacgacct tgtttggcag aaccaccggc agagtccccc tcaactagat agagttcatt
     5461 cttagcagga ttcttagatt gggctggggt caatttccca gacaacaagc ccttatcttt
     5521 cttgtttttc ttcccatttc ggctctcatc acgcgcctta cgtgctgctt cacgagcatc
     5581 acgggccttg atagccttgc ggatgaggtt agaagctaat tccccatttt ccataaggaa
     5641 aaaggtcaac ttatcagcca ctattccatc cacaactggg cgagctaggg ggcttcctag
     5701 tttatccttg gtctgtcctt caaactgcaa gtgttcttca ggaactaaga tagaaagaac
     5761 ggccgctagt ccctcacgat agtctgaacc ttcaaggttt ttatcttttt ccttgagaag
     5821 acctgtttta cgtgcatagt cattcatgac cttggtaatg gcagacttga gtcctgtctc
     5881 gtgcgttcca ccgtccttgg tgcgaacgtt attgacaaag gatagaatgt tatctgagaa
     5941 tccgtcattg tactggaggg ctacttccac ttgaaaacca ttgtcttccc cttcaaagta
     6001 aagaactggc gtcaagattt ccttatcttc gttgagataa gaaacaaaat cttgtactcc
     6061 attctcatag tggaactcaa tcgcttcatc tgttcgcttg tccgttaaag acaaggtcac
     6121 atttttcaag agaaaggctg attcattaag gcgctctgaa atggtattgt acttgaaatc
     6181 tgtcgtagaa aagatagtcg cgtcaggcat aaaagtaact ttggtgcctg ttttagactt
     6241 gggtgctgta ccgattttct tcaaagtcgt gacaggtttt ccaccatttt caaaacgttg
     6301 cttgtaaact gcgccatcac gggtaatttc aacttctaac cagctagaaa gggcgttaac
     6361 aacggaagaa cccactccgt gaagtccacc tgatgtctta tagccacctt gaccgaattt
     6421 ccctccggca tgaagaatgg taaagataac ctcaacagtt ggaattccca tagcgtgcat
     6481 acctgtcggc atcccacgtc catggtcttg aaccgttaga ctaccgtctt tattgatggt
     6541 tacatcgata cgatcaccaa acccagacaa ggcttcatcg actgcattat caacgatttc
     6601 ccaaactagg tgatgaagac cagcgccatc ggtcgatcca atatacatcc ctggacgttt
     6661 tcggaccgca tccaaccctt ctagcacctg aatagcatca tcattataat tgttaatatt
     6721 gatttccttt tttgacacaa ggaacctcct attcgttcat ctttactatt ctacaggttt
     6781 tccaaggatt ttgcaaaatt tttctttctc cgatgtgaca attttagcag agattctctg
     6841 cttttctttc ccaattcatg atataatagg agtatgatta caatagtttt attaatccta
     6901 gcctatctgc tgggttcgat tccgtctggt ctctggattg gacaagtatt ctttcaaatc
     6961 aatctgcgtg aacatggttc tggaaatacc ggaacgacca ataccttccg cattttaggc
     7021 aagaaagctg gtatggcaac ctttgtcatt gactttttca aaggaaccct agcaacgctt
     7081 cttccgatta tgtttcatct acaaggcgtt tcgcctctca tctttggact tttagctgta
     7141 atcggccata ccttccctat ctttgcagga tttaagggtg gtaaggctgt cgcaaccagt
     7201 gctggagtgg ttttcggatt tgcgcctgtc ttctgtctct accttgcagt tgttttcttt
     7261 ggaaccctct atctaggcag tatgatttca ctttccagtg tcacagcatc tatcgcggct
     7321 gtcatcggag ttctactctt tccacttttt ggttttatcc tgaacaacta tgaccctctc
     7381 ttcatcgcta ttatcttagc acttgctagt ttgattatca ttcgtcataa ggacaatata
     7441 gctcgtatca aaaataaaac tgaaaatttg gtcccttggg gattgaacct aacccatcaa
     7501 gatcctaaaa aataaaatgc cagttctgtg aactgcaccc caaaagttag acaattaatt
     7561 tatccgaagg atttagttct gtattgcaca gggctaagtc cttttagttt taccttaatt
     7621 cgtttattgt tgtagtaatc aatatagtct ataatagctt gttccaattg cttaaacgac
     7681 tgaaacgact tctcataacc gtaaaacatt tccgatttca gaatcccaaa gaaggactcc
     7741 atcataccat tgtctgggct gttaccctta cgtgacatgg atgcttgaat tcccttactc
     7801 tctaggaacc gatgataaga atcgtgttga tattgccagc cttggtcact atggagaatc
     7861 gtattctcgt agtgcttctc tttgaatgcc tgttccaaca ttgtttgtac ttgttctaaa
     7921 ttaggcgaac aagaaagatt aaaagcaata atttcactgt taaagccatc taaaactggt
     7981 ggtaagtaaa gcttttgagt acttgctgga atggcaaatt ctgtcacatc tgtgtagcac
     8041 ttttccattg ttttagagcc ttcaaattgg ccttgaatga gattctctgc cttcttgcca
     8101 acgtctcctt tatgagaaga atattttcgt ttctgtcgca ttttagcttg taaattgagt
     8161 acttttatca agccttgaac tcttttatga tttaccagat aagcacgatt tcttagttct
     8221 aaatgaaccc g
//
LOCUS       DAVHIU010000081         8040 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVHIU010000081 DAVHIU010000000
VERSION     DAVHIU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.083"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            80..457
                     /gene="mscL"
                     /locus_tag="VVN06_001529"
     CDS             80..457
                     /gene="mscL"
                     /locus_tag="VVN06_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU5563040.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKTAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(499..1593)
                     /gene="hemH"
                     /locus_tag="VVN06_001530"
     CDS             complement(499..1593)
                     /gene="hemH"
                     /locus_tag="VVN06_001530"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5563041.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESQQIRFLVIKDWYQQQSLLDFWTDEIRKILRNEVGEE
                     SFKVIFSAHSVPIFALDYGDPYIDQIFDNSRLIAEQLGLMTDQYTNTWQSESDIGIPW
                     IKPDVLEYLREQKQHPEHYIFVPISFISEHIEVLFDNDVECYDLCQELGVTYHRPPMP
                     NTDSRLIDALVATVRANEDKEFKTFLPEEETFDELAPSATTKDIMKETDDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(1671..2457)
                     /gene="srtB"
                     /locus_tag="VVN06_001531"
                     /pseudo
     CDS             complement(1671..2457)
                     /gene="srtB"
                     /locus_tag="VVN06_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(2572..3570)
                     /gene="srtG1"
                     /locus_tag="VVN06_001532"
     CDS             complement(2572..3570)
                     /gene="srtG1"
                     /locus_tag="VVN06_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HEU5563042.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(3588..4784)
                     /gene="pitB"
                     /locus_tag="VVN06_001533"
     CDS             complement(3588..4784)
                     /gene="pitB"
                     /locus_tag="VVN06_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEU5563043.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(4829..5380)
                     /gene="sipA"
                     /locus_tag="VVN06_001534"
     CDS             complement(4829..5380)
                     /gene="sipA"
                     /locus_tag="VVN06_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEU5563044.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLATL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFSMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(5380..7977)
                     /gene="pitA"
                     /locus_tag="VVN06_001535"
                     /pseudo
     CDS             complement(5380..7977)
                     /gene="pitA"
                     /locus_tag="VVN06_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516426.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus tip adhesin PitA"
BASE COUNT         2237 a         1658 c         1506 g         2639 t
ORIGIN      
        1 ctccatttat gagcctttta attgccttct ataatgattt aggatacaat gaaacacgaa
       61 acattgaaag gaaaacagta tgttaaaaaa tctaaaatcg ttcttgcttc gaggaaatgt
      121 tattgacctt gctgtcggtg ttgtcattgc ctctgctttt ggtgctatcg ttacttcact
      181 tgtaaacgac attatcactc ctcttatttt aaatcccgct ttgaaaactg ctaaagtcga
      241 acgtatcgct caactttctt ggcatggagt cggctatggt aactttttaa gtgctattat
      301 caattttatc tttgtgggta ccgccctctt ctttattatc aagggcattg aaaaagcaca
      361 gaaactgact ggcataaagg aagaaaaaac tgccgaaaaa aaaccaaccg aattggaagt
      421 ccttcaagaa ataaaagctc tccttgagaa aaaataatcg attaaaagga tctagcgcaa
      481 agctaaatcc ttttttcttt actctttggg taacttgcct gctttttcaa gcattttctt
      541 gatcagataa ggcatcttaa cattttcacg accttttttc tcaatgagtt ttttgacaaa
      601 ttctggcatc tgaaggtcgt ctgtctcttt catgatgtcc ttggtagttg ccgaaggcgc
      661 taactcatca aaggtttctt cttctgggag aaaagtttta aattctttgt cttcgttggc
      721 tcttacagta gcaactaaag cgtcaattaa acggctatcc gtattgggca tcggtggacg
      781 atggtaagtg acacctaatt cttgacacaa atcataacat tccacatcat tatcgaacaa
      841 gacttcgatg tgctcactga taaagctaat cggaacaaaa atataatgtt ctggatgttg
      901 cttttgttct cgtaaatatt ctaagacatc tggcttgatc caagggattc caatatcgct
      961 ttcgctctgc caagtattgg tatactggtc tgtcattagg cctagttgtt cagcaatgag
     1021 cctgctatta tcgaaaatct gatcgatata aggatctcca tagtccaagg caaaaatggg
     1081 aacgctatgg gctgaaaaga ttaccttgaa ggattcttct cccacctcat ttcgtaaaat
     1141 ttttctaatc tcatctgtcc agaagtccag caaagactgt tgttgatacc agtccttaat
     1201 aactaaaaat cggatctgct ggctttccag aaacttttca taccccatga ctgagtaaaa
     1261 agaataatga ggttccaaaa tcaagcagat acattcttca acaccatcag tttccatctg
     1321 cttgatcacg tctggaataa agggacgaga aaatttgttg gcaaagtaga taccgtactc
     1381 ctcccctaaa cgttctttga ctaaattgac ctcctcacgt gtaatccgct gcaaaggcgt
     1441 cccaccgata cgaatatagt tgtcatagag agtctggatt tcgtggtctt ggggtctaac
     1501 tccacgacga atgtttgtaa agaattctgc tactccttca aaactaatct cctctggaga
     1561 tccaaaggtc atcattaata tggctttttt catgttatca tccttatgat attattttct
     1621 ctatccattc tatcatgaaa gaaaaaaata gtaaacgttt cctacgtcta ctatttcttt
     1681 tgctcttttg tttttttatc tcgtcttata attaggatga taaggacaag aagtgggaat
     1741 tccgtgatcg aaataccgtg ttcctactgc ttccccactt tcatcctgtg ctactttcac
     1801 cggtgcatcc gttattttag ctaaaaggat atgtcgtcca ttagttatgt taaggaaaca
     1861 ggtgcttaat aaaacaatct tgtccgttga ggtcaaggaa acatctcgct tgtacttggc
     1921 tttacttagc agatattgat agtaagcctg gtgttcctca tccttggaac tcaaggttcg
     1981 ataaatctcc gtgtcatagg catccacttc taaaatccca aaaatttcaa gaccacgttc
     2041 tcgaccattg tagtagatag aaccgtagcg atgctggtcg aagaattcct tatcagcaaa
     2101 tttcttaatc tcaccgaaca tagcccctga agccatagag tgtccgtaga tgatcgtatt
     2161 aaaatcagta aagttgggat taaagcgata gtcgagaaaa attgatcccg ttatcgtata
     2221 atcgccaaag gcattgcggt caaggtattt gacattatcc tgactttgta gaagcggata
     2281 gtctaccttg gtcccatcaa ggctaatcca accgcaaaca tctttgttca agtgctgtag
     2341 ggtttcaaaa cctgtgctcg aagcatcctt ttcaatagga ttatagtgat catagtcact
     2401 tgcttctact actctctgcc aactaaaaag agctacaaaa acacagaaga ataccagaaa
     2461 tacgttttta aatcgcgctc ttttgaaccg tcatcagcac tcctttctat gcgagatttc
     2521 ttaaaaggaa aggaggcggg acaaaaccgc ctcttttcta ttcaccctta gtcagtaaaa
     2581 gggtctgctt cttgcatttt ttttactctc tcttctacga cgtaggatca agtagaggac
     2641 aaggagtaag aaaatcaaga gcaagaacaa tacaaacaag ataaggtacc aaatccatag
     2701 tgggattgat gagaggaaac gtccaagaga cgagtcatca aagacactgt atggaaatgg
     2761 ttttgatttt tttgtatgga aagtattttt agggacagtg tctgtaatct ttgcgactac
     2821 gatatgacga ccattggtca catcgagaaa acaggtactg agtaggatga tacgatcact
     2881 cggtgagagt gagatatccc gcttgtgcat ggcgactgag agcaggtggt ctagataggc
     2941 ctgctggcgg tcctcaccct gtattcctgg atcatagatg ttaaagtcat aggcatcaac
     3001 ctccaacatc tcaaagatct cgagcccttt ttccacacca ttgtagtata tactaccgta
     3061 acgatgctgg tcaaaaaatt cctgatcagc aaacttagcc acatcaccaa acatgacccc
     3121 attttctacg tggtgcccgt agataatggt attaaagtct tcgaacttag gattatttcg
     3181 tgcatcgaga aagatagcgc ctgtcgctgc aaactcacct ttggagtcct tgttgagata
     3241 cttttcattg tccttggctt ggactaaggg atagtcgata ttggtgccgt agacattgat
     3301 ccaaccgagg acttcgggat tgagttcttg gagtttgctg aagcctgaaa aactggccag
     3361 ctcatcctgt tgcgtcgtga caggtcgata agcttcgtac tcacttgacg aagctaagga
     3421 gtagacttgg ttggaatccc aaagggcgta gaaaccaaag agaaagatca aagctgcaaa
     3481 aatgagcagc aagtgattga tcaccccatc cacagcaaga acgagacgcc tagacagact
     3541 cttttttggt ttctcacgct tggttttcat catctgcccc cagccgttta ggcttctcta
     3601 cgttttttag acaagacaac aaatccagcc aaaccaagac caatcatcaa aatgaatgga
     3661 aggttatcaa tcaagagacc agtaggagta acgtcgtcga ttttgttagt aactttgttg
     3721 tcgtttttct tttcacctat gaggatatct gttaatgtaa aatctacatt tttttgtcct
     3781 tgagatgttt gttctgcacc atttgtagta taaattgatg aatctgtata acctgctact
     3841 ccagtttcag ttactgtata tttagaacct gctggtagtt gttcaactgt aaatgtttca
     3901 ccatctttaa gagtaaaatt gtactcaact gtatctgctg gggtcactac tgccaacacc
     3961 tctgacttag atccatgttt tactataata ttcgtaacag gggtttctgc tgttttattt
     4021 gtacttggaa gttgaatctt gatatggaat tggaaatctc ttgttttatc ccctgaagct
     4081 ccatcaactt ttttagtaat tgttaatgct ttcgcattgg ggtctacttg actcggatca
     4141 tcaggattag gctccttact tgcgtctttg gtatatgtgt tatcaaataa attataaacg
     4201 ccttgttcac ttggttctac tttaggggcg ttagatttat tattttcctt aaagtatact
     4261 gatgaaatat agacaccacc atttacttta ttcttaacaa taacgtgcat ttcatatgta
     4321 cgtttgtcgt atctcatggc atcaattgtt ttaccatttt tggtaatcgc ctgccatcca
     4381 gtttttgctt ccgctacatc ataaacgtat tcaccagcat ggccataagc aactcccgaa
     4441 aaaatgtctt ctgttgcttt ttgaatctta tcagcagttg ggtgatcatt tttattgtat
     4501 tctacatttc tatctggaat tgcaactccg gtttgatatg gcgcaccgtt agaagattgt
     4561 cctgtttttt ctgtaaactt aaatgtaaat gtcgctgttg gtgttgttat tccctctgca
     4621 atattcaacg ttttattaat cttcacaaca gcattatttt caccatttgc tttggttatt
     4681 gctgaattat catccgcaaa agtcgggaca cctctgaata cggacaaagc ggttatagct
     4741 gcaatgcttg cagtgaatag ctttttaaag aatgtttttt tcatgattat ttcttttgtt
     4801 tttttatttt cttttttcat tatgatcatc aaaagcctcg tcttctaatt actgttacaa
     4861 cttttccttg agtatcctgt atagggatgg ttccaaaagc acgactgtct acagccgttg
     4921 ttcgattgtc cccgaggaca aaaagttgtc ctgctgggac tttcatcgag aaggttgccc
     4981 cttccttata gagcagcgtt tctttgtaga tatcttgctc ttgttgagga gaaccgttga
     5041 tgatgagacc attttcgttt atatcgattg tacttccttc ggttgctatg acacgcgcca
     5101 ctctttcctt acctttataa ctatagacta gcaaatcacc aatcgaatag cgtttatcca
     5161 acctataata gacgaccaag tcgccatctt ttaaggcggg cttcatgcca tcgtcattgt
     5221 atcttaataa tccaaagacg aaaaagtaaa gggtggctag tacaaatcca accatcagaa
     5281 gtttactgac taagtaaagg atatctcccc agacactcgg cggagactta tcccgattga
     5341 cttgtgttac tggtttctta tgtttctttt taagcagcat cacttcctcc tattcaccac
     5401 aaggtgacca agtaagatga taaagaagat ccctcctaga ggaagaacga ctccaagcac
     5461 gagagtgcta ctcaagaagt caattcccgt ttcaggtacg ctgtttttat tattcgtgac
     5521 tgtcgcagac ttatttgcat caagagttcc ttctttttta tcataggaca cctgataccc
     5581 acgagacgaa cttgcttctt cttcgatact ataacgagca tttagaggaa ggtcgagaat
     5641 cttgatggtt ttatctttct ttagatcaac tgtcgcctta ccattggtga attgtagcgt
     5701 tgttttttga ttatttacta ttgcactata cgatccattc aatggtttat tttgcgcatc
     5761 ctttacgtta atggtaatct taaaggattg cagaagatta gcaaaagcac ctgtaacttt
     5821 ttttgtgact gaaagtgtcg gaagtctgtg gttggtgacg attgtttgaa catcctctga
     5881 caaagtcccc tcttgttttt cataggtgac atgatatccc gttctagagt tctctacttc
     5941 ttccacctta tagtgggtat cacgaggcag ataactgagg gcagtttcct gactattttt
     6001 gagagaaatc tgtgctttcc cgttagtcac tgttgtattc ccaaacttgc cgttaatcgg
     6061 cttgccatcc ttatctgaaa gggtaacatt aattgtgaaa gatttattag gtgattgttc
     6121 accttctagg attttcttta cggtgagact tggaagctgt cggtaaataa ctttgaagga
     6181 tggtttctct ttcgtacctg tcttttgaac ttcgactgtg tatcccggag cgtctggtgc
     6241 tttcagttcg tagtcatatc ccttagctac ctttgcaaaa ctagtatttt cagtaaaact
     6301 attcgtttga actggactag tgacctttcg ataggaatcc tttatacttc catctttctt
     6361 ttggtttaag tttaattcga cactaggtgg acgatttgct gatggatttg attttccatc
     6421 cacacctttc cactcaatct ctacaggaac cgtcaatgga tctgacggct tgaaagtggg
     6481 cttctcagaa tagtctttta ttttggtacg tgaggtccca ataccatagg aataggcaac
     6541 ttttgccccc ttgttggagt agagtttagt agcatcggct tcttcggcat catcactagt
     6601 aagtgtctta tcacccgcaa tcgcatcaaa tgcctcttgg ctagaggtga cagtaaactt
     6661 gagaacgtac ttggcgtcat cctccaaagt gtaactcgga ttaaacttgg ctgttacttc
     6721 cactaatccc tgttcgttct tcttagtttc aatcgtaact tgattgtcat tcagttcttg
     6781 ctcgttacca tccttgattt tgacgacacg aaggtgtgaa gcatcaccag gtaacagctg
     6841 cacgtacttg gacaagacat ctgatatagt tacatggtgt acgcctagag gtagaatttt
     6901 atctgtgatt tctttgaatt tttgttgcaa gtcattttca ttattggcag aaaatatttc
     6961 gttggggatt ccagaattat aaccactgat atatccttta agagatgtga tactatcgtt
     7021 tgaacctata tatctgaatt tgattgaata aaagccatct atttttggag ctattaaact
     7081 agatatattc agcattcctc tctctaaacc agaggtaaac caatatggag tatcaatcat
     7141 acgtcccaca tttggattag aataatagtt atatattgtt ctcccactat tagactcgta
     7201 atacatatta gcttcgccat ctgacagcag aataactact ttttttgccc cagcccttgc
     7261 actttgcaac aatctttgag cttcagttaa cccagcattt atatttgtcc cagtgccaat
     7321 gcccgcgtcc ataccataag aatgactaga gtcagcaatt gttaacgaac taacaatatt
     7381 ttttgagcta ttagaatccg tgctccaaga tactaaagtt ttagcatcat catatgaaga
     7441 gatatttgaa taccaaccag ctcaatttgg ccaagttcca tattgataac tcagataata
     7501 ttcattataa taacggccat tatactgatt atcaatcttt ccggcaaaac caactataga
     7561 cagacggttt tgggaattag aaagaattgt atcaatgagc ccctgacgac cattcacacc
     7621 tttcagtgta tgttttagtg cttcaatcct actaatgtat cgtctactag agtaagacca
     7681 cacatctcgc tcttccatac tccctgaaaa atcggcaacc aagactacat ccaagggttc
     7741 tgactgggtc tccgtctggg tctccgttcc cagtttagat gtgatatcca gtgacaactc
     7801 atactggtct tcttgccctg atatcggagt aatcgttttg tgcagagttg tctgaggttc
     7861 agttgttgaa tctgcacgta aagaattaat tggtgcacac aaaaaaagaa tagcgagaaa
     7921 gataactaaa ctactggtaa aatattgaat cttcttaaaa tcatattgaa cgttcatgct
     7981 atctcccttg ctagaatatg ttttaatctg atactatata aatctgagac ttccataaga
//
LOCUS       DAVHIU010000082         7974 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVHIU010000082 DAVHIU010000000
VERSION     DAVHIU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.084"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            25..567
                     /gene="hpt"
                     /locus_tag="VVN06_001536"
     CDS             25..567
                     /gene="hpt"
                     /locus_tag="VVN06_001536"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5563045.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            583..2541
                     /gene="ftsH"
                     /locus_tag="VVN06_001537"
     CDS             583..2541
                     /gene="ftsH"
                     /locus_tag="VVN06_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5563046.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            2663..3142
                     /locus_tag="VVN06_001538"
     CDS             2663..3142
                     /locus_tag="VVN06_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5563047.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            3236..3307
                     /locus_tag="VVN06_001539"
     tRNA            3236..3307
                     /locus_tag="VVN06_001539"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3269..3271,aa:Glu,seq:ttc)
     gene            3559..5104
                     /locus_tag="VVN06_001540"
     rRNA            3559..5104
                     /locus_tag="VVN06_001540"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            5156..5228
                     /locus_tag="VVN06_001541"
     tRNA            5156..5228
                     /locus_tag="VVN06_001541"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5189..5191,aa:Ala,seq:tgc)
     gene            5353..>7974
                     /locus_tag="VVN06_001542"
     rRNA            5353..>7974
                     /locus_tag="VVN06_001542"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2455 a         1459 c         2113 g         1947 t
ORIGIN      
        1 atagaaaaaa tagataggta aaaaatgtta gaaaacgata ttaaaaaagt cctcgtttca
       61 cacgatgaaa ttacagaagc agctaaaaaa ctaggtgctc aattaactaa agattatgca
      121 ggaaaaaatc caatcttagt tgggatttta aaaggatcta ttccttttat ggctgaattg
      181 gtcaaacata ttgatacaca tattgaaatg gacttcatga tggtttctag ctaccatggt
      241 ggaacagcaa gtagtggtgt tatcaatatt aaacaagatg tgactcaaga tatcaaagga
      301 agacatgttc tatttgtaga agatatcatt gatacaggtc aaactttgaa gaatttgcga
      361 gatatgttta aagcaagaga agcagcttct gttaaaattg caaccttgtt ggataaacca
      421 gaaggacgtg ttgtagaaat tgaggcagac tatacctgct ttactatccc aaatgagttt
      481 gtagtaggtt atggtttaga ctacaaagaa aattatcgta atcttcctta tattggagta
      541 ttgaaagagg aagtgtattc aaattagaaa gaataatctt taatgaaaaa acaaaataat
      601 ggtttaatta aaaatccttt tctatggtta ttatttatct ttttccttgt gacaggattc
      661 cagtatttct attctgggaa taactcagga ggaagtcagc aaatcaacta tactgagttg
      721 gtacaagaaa ttaccgatgg taatgtaaaa gaattaactt accaaccaaa tggtagtgtt
      781 atcgaagttt ctggtgtcta taaaaatcct aaaacaagta aagaaggaac aggtattcag
      841 tttttcacgc catctgttac taaggtagag aaatttacca gcactattct tcctgcagat
      901 actaccgtat cagaattgca aaaacttgct actgaccata aagcagaagt aactgttaag
      961 catgaaagtt caagtggtat atggattaat ctactcgtat ccattgtgcc atttggaatt
     1021 ctattcttct tcctattctc tatgatggga aatatgggag gaggcaatgg ccgtaatcca
     1081 atgagttttg gacgtagtaa ggctaaagca gcaaataaag aagatattaa agtaagattt
     1141 tcagatgttg ctggagctga ggaagaaaaa caagaactag ttgaagttgt tgagttctta
     1201 aaagatccaa aacgattcac aaaacttgga gcccgtattc cagcaggtgt tcttttggag
     1261 ggacctccgg ggacaggtaa gactttgctt gctaaggcag tcgctggaga agcaggtgtt
     1321 ccattcttta gtatctcagg ttctgacttt gtagaaatgt ttgtcggagt tggagctagt
     1381 cgtgttcgct ctctttttga ggatgccaaa aaagcagcac cagctatcat ctttatcgat
     1441 gaaattgatg ctgttggacg tcaacgtgga gtcggtctcg gcggaggtaa tgacgaacgt
     1501 gaacaaacct tgaaccaact tttgattgag atggatggtt ttgagggaaa tgaagggatt
     1561 atcgtcatcg ctgcgacaaa ccgttcagat gtacttgatc ctgccctttt gcgtccagga
     1621 cgttttgata gaaaagtatt ggttggccgt cctgatgtta aaggtcgtga agcaatcttg
     1681 aaagttcacg ctaagaacaa gcctttagca gaagatgttg atttgaaatt agtggctcaa
     1741 caaactccag gctttgttgg tgctgattta gagaatgtct tgaatgaagc agctttagtt
     1801 gctgctcgtc gcaataaatc gataattgat gcttcagata ttgatgaagc agaagataga
     1861 gttattgctg gaccttctaa gaaagataag acagtttcac aaaaagaacg agaattggtt
     1921 gcttaccatg aggcaggaca taccattgtt ggtctagtct tgtcgaatgc tcgcgttgtc
     1981 cataaggtta caattgtacc acgcggccgt gcaggcggat acatgattgc acttcctaaa
     2041 gaggatcaaa tgcttctatc taaagaagat atgaaagagc aattggctgg cttaatgggt
     2101 ggacgtgtag ctgaagaaat tatctttaat gtccaaacta caggagcttc aaacgacttt
     2161 gaacaagcga cacaaatggc acgtgcaatg gttacagagt acggtatgag tgaaaaactt
     2221 ggcccagtac aatatgaagg aaaccatgct atgcttggtg cacagagtcc tcaaaaatca
     2281 atttcagaac aaacagctta tgaaattgat gaagaggttc gttcattatt aaatgaggca
     2341 cgaaataaag ctgctgaaat tattcagtca aatcgtgaaa ctcacaagtt aattgcagaa
     2401 gcattattga aatacgaaac attggatagt acacaaatta aagctcttta cgaaacagga
     2461 aagatgcctg aagcagtaga agaggaatct catgcactat cctatgatga agtaaagtca
     2521 aaaatgaatg acgaaaaata accctgagag aggctggagc ctctcttttt tgtgcagttt
     2581 aggagctaaa gggaacagaa tggagaaaat ggaacaaatg tgttttctaa tctgttagac
     2641 tgtatctaga aaggggaaaa ttatgattaa agaattgtat gaagaagtcc aagggactgt
     2701 ttataagtgt agaaatgaat attaccttca tttatgggaa ttgtcggatt gggaccaaga
     2761 aggcatgctc tgcttacatg aattgattag tagagaagaa ggactggtag acgatattcc
     2821 acgtttaagg aaatatttca aaaccaagtt tcgaaatcga attttagact atatccgtaa
     2881 gcaggaaagt cagaagcgta gatacgataa agaaccctat gaagaagtgg gagagatcag
     2941 tcatcgtata agtgaggggg gtctctggct agatgattat tatctctttc atgaaacact
     3001 aagagattat agaaacaaac aaagtaaaga gaaacaagaa gaactagaac gcgtcttaag
     3061 caatgaacga tttcgagggc gtcaaagagt attaagagac ttacgcattg tgtttaagga
     3121 gtttactatc cgtacccact agtaagtcat gcaaaaaaaa tgaaaaaaat tagaaaaagt
     3181 agttgacaaa gtttgaaaag actgtataat agtaagagtt gaaaataaca actcaggtcc
     3241 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
     3301 cggactatgg tatgttgcgg ttggaacact tgatgaaaaa aagtttaaaa aagcttaaaa
     3361 atcttcaaaa aagtgttgac aagcgaaagc ggctgtgata tactaatata gttgtcactt
     3421 gagagaagca agtgacaaag acctttgaaa actgaacaag acgaaccaat gtgcagggcg
     3481 ctacaacata agttgtagta ctgaacaatg aaaaaacaat aaatctgtca gtgacagaaa
     3541 tgagtgagaa ctcaaacttt taatgagagt ttgatcctgg ctcaggacga acgctggcgg
     3601 cgtgcctaat acatgcaagt agaacgctga aggaggagct tgcttctctg gatgagttgc
     3661 gaacgggtga gtaacgcgta ggtaacctgc ctggtagcgg gggataacta ttggaaacga
     3721 tagctaatac cgcataagag tggatgttgc atgacatttg cttaaaaggt gcacttgcat
     3781 cactaccaga tggacctgcg ttgtattagc tagttggtgg ggtaagggct caccaaggcg
     3841 acgatacata gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga
     3901 ctcctacggg aggcagcagt agggaatctt cggcaatgga cggaagtctg accgagcaac
     3961 gccgcgtgag tgaagaaggt tttcggatcg taaagctctg ttgtaagaga agaacgagtg
     4021 tgagagtgga aagttcacac tgtgacggta tcttaccaga aagggacggc taactacgtg
     4081 ccagcagccg cggtaatacg taggtcccga gcgttgtccg gatttattgg gcgtaaagcg
     4141 agcgcaggcg gttagataag tctgaagtta aaggctgtgg cttaaccata gtaggctttg
     4201 gaaactgttt aacttgagtg caagagggga gagtggaatt ccatgtgtag cggtgaaatg
     4261 cgtagatata tggaggaaca ccggtggcga aagcggctct ctggcttgta actgacgctg
     4321 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gctgtaaacg
     4381 atgagtgcta ggtgttagac cctttccggg gtttagtgcc gtagctaacg cattaagcac
     4441 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca
     4501 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat
     4561 ccctctgacc gctctagaga tagagttttc cttcgggaca gaggtgacag gtggtgcatg
     4621 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccccta
     4681 ttgttagttg ccatcattta gttgggcact ctagcgagac tgccggtaat aaaccggagg
     4741 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca
     4801 atggctggta caacgagtcg caagccggtg acggcaagct aatctcttaa agccagtctc
     4861 agttcggatt gtaggctgca actcgcctac atgaagtcgg aatcgctagt aatcgcggat
     4921 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga
     4981 gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc cagccgccta aggtgggata
     5041 gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct ggatcacctc
     5101 ctttctaagg ataaggaact gcgcattggt cttgtttagt cttgagaggt cttgtggggc
     5161 cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt cgatcccgct
     5221 aggctccatt ggtgagagat caccaagtaa tgcacattga aaattgaata tctatatcaa
     5281 atagtaacaa gaaaataaac cgaaaacgct gtagtattaa taagagttta tgactgaaag
     5341 gtcagaaaat aaggttaagt taataagggc gcacggtgga tgccttggca ctaggagccg
     5401 acgaaggacg tgacaaacga cgatatacct tgggtagctg taagtaagcg atgatccagg
     5461 gatttccgaa tgggggaacc caacaggtaa tacctgttac ccacatctgt taaggatgtg
     5521 aggaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa agcaaaagcg
     5581 attgccttag tagcggcgag cgaaacggca gaagggcaaa ccgaagagtt tactcttcgg
     5641 ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga atgatttggg aagatcagcc
     5701 aaagagagta atagcctcgt atttaaaata gtctttgtac ctagcagtat cctgagtacg
     5761 gcgggacacg tgaaatcccg tcggaatctg ggaggaccat ctcccaaccc taaatactcc
     5821 ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cgggagggga
     5881 gtgaaataga acctgaaacc gtgtgcctac aacaagttcg agcccgttaa tgggtgagag
     5941 cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat gcgaggttaa gttgaagaga
     6001 cggagccgta gggaaaccga gtctgaacag ggcgaattag tatcatgacg tagacccgaa
     6061 accatgtgac ctacccatga gcaggttgaa ggtgcggtaa gacgcactgg aggaccgaac
     6121 cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc ggagaaattc caaacgaact
     6181 tggagatagc tggttctctc cgaaatagct ttagggctag cgtcgacatt agagattctt
     6241 ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc agataaactc
     6301 cgaatgccaa tgaattatgg tcggcagtca gactgcgagt gctaagatcc gtagtcgaaa
     6361 gggaaacagc ccagaccacc agctaaggtc ccaaaataat tgttaagtgg aaaaggatgt
     6421 ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca aagagtgcgt
     6481 aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa acaatttacc
     6541 gaagctgtgg atacctttat aggtatggta ggagagcgtt ctatgtgtga tgaaggtata
     6601 ccgtgaggag tgctggaacg catagaagtg agaatgccgg tatgagtagc gaaagacagg
     6661 tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc gccctgggtt
     6721 agtcgggacc taaggagaga ccgaaaggtg tatccgatgg acaacaggtt gatattcctg
     6781 tactagagta tgtagtgatg gagggacgca gtaggctaac taaagcagac gattggaaga
     6841 gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt aattctataa cattgagctg
     6901 tgatggggag cgaagtttag tagcgaagtt agtgacgtca cactgccaag aaaagcttct
     6961 agcgtttaaa catactctac ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc
     7021 tcaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt aacttcggga
     7081 gaaggggtgc tgactttagg tcagccgcag tgaataggcc caagcaactg tttatcaaaa
     7141 acacagctct ctgctaaatc gtaagatgat gtataggggg tgacgcctgc ccggtgctgg
     7201 aaggttaaga ggagtgctta gcgtaagcga aggtatgaat tgaagcccca gtaaacggcg
     7261 gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacccgca
     7321 cgaaaggcgt aatgatttgg gcactgtctc aacgagagac tcggtgaaat tttagtacct
     7381 gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt actgcagttt
     7441 gatattgagt gtctgtacca catgtacagg ataggtagga gtctaagaga tcgggacgcc
     7501 agtttcgaag gagacgctgt tgggatacta cccttgtgtt atggccactc taacccagat
     7561 aggtgatccc tatcggagac agtgtctgac gggcagtttg actggggcgg tcgcctccta
     7621 aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt cgcagagtgt
     7681 aaaggtataa gggagcttga ctgcgagagc tacaactcga gcagggacga aagtcgggct
     7741 tagtgatccg gtggttccgt atggaagggc catcgctcaa cggataaaag ctaccctggg
     7801 gataacaggc ttatctcccc caagagttca catcgacggg gaggtttggc acctcgatgt
     7861 cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa
     7921 gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc cgtc
//
LOCUS       DAVHIU010000083         7908 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVHIU010000083 DAVHIU010000000
VERSION     DAVHIU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.085"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..201)
                     /locus_tag="VVN06_001543"
     CDS             complement(<1..201)
                     /locus_tag="VVN06_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563048.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGTNNFSLPNVSPTYGQYY"
     gene            complement(351..617)
                     /locus_tag="VVN06_001544"
                     /pseudo
     CDS             complement(351..617)
                     /locus_tag="VVN06_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(642..953)
                     /locus_tag="VVN06_001545"
     CDS             complement(642..953)
                     /locus_tag="VVN06_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU5563049.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(878..1120)
                     /locus_tag="VVN06_001546"
                     /pseudo
     CDS             complement(878..1120)
                     /locus_tag="VVN06_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1121..1753)
                     /locus_tag="VVN06_001547"
     CDS             complement(1121..1753)
                     /locus_tag="VVN06_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563050.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(1767..2762)
                     /locus_tag="VVN06_001548"
     CDS             complement(1767..2762)
                     /locus_tag="VVN06_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5563051.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2759..3457)
                     /locus_tag="VVN06_001549"
     CDS             complement(2759..3457)
                     /locus_tag="VVN06_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU5563052.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KIVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(3535..4545)
                     /locus_tag="VVN06_001550"
     CDS             complement(3535..4545)
                     /locus_tag="VVN06_001550"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU5563053.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAETCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGFQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(4529..5536)
                     /locus_tag="VVN06_001551"
     CDS             complement(4529..5536)
                     /locus_tag="VVN06_001551"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU5563054.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLAIYGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIAAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(5523..6476)
                     /gene="mvaD"
                     /locus_tag="VVN06_001552"
     CDS             complement(5523..6476)
                     /gene="mvaD"
                     /locus_tag="VVN06_001552"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU5563055.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(6458..7336)
                     /gene="mvk"
                     /locus_tag="VVN06_001553"
     CDS             complement(6458..7336)
                     /gene="mvk"
                     /locus_tag="VVN06_001553"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU5563056.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVS
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(7458..7787)
                     /locus_tag="VVN06_001554"
     CDS             complement(7458..7787)
                     /locus_tag="VVN06_001554"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5563057.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2278 a         1863 c         1467 g         2300 t
ORIGIN      
        1 ataatattga ccataagtcg gtgaaacatt gggaagtgaa aaattattag ttcctggact
       61 atcttgtaat gtctcactta cctcatattc ttcacctgta ctagaaataa cactcaaagc
      121 agatactgtc gataactggc tagccaataa agtactcgca ataattgaaa tacccaattt
      181 tttataaaca gttttcttca ttattgtatc ctcctaatgt aattatagcg tactattcta
      241 aatttcttaa tctactatag aatcaagaaa tctaccacct tctttaaata ccctccatta
      301 tcacataaac aggtaaactt ttcaattaat gactgcgctt ttcaatcacg ctagaggtac
      361 ttacttgctt ctttgatact aagttcagcc attctttcct tgtttttctc aataaagcat
      421 gttacccaag tgggattcgt tttggagtag tctcgcagag tccagccaat ggctttattg
      481 ataaaaaatt ctgtttggtt caagttatga agtagaatct tttccattaa ttgagtattg
      541 gtcttctctt ttcttaacaa ctggtggtca atagcgacac gtctcagcca gatattatct
      601 gataggctcc attttatact caataaaaat caaagagcaa actaggaagc tagccgcaga
      661 ttgctcaaaa cactgttttg aggttgcaga tagagctgac gtggtttgaa gagattttcg
      721 aagagtatta agattatttc ttctagttca gggtgttcat acaccaaact ccctactact
      781 cgatctagga tatctaccgt gtcccaccag gattttgtca cgactaactg ctctagctta
      841 ggcaaatcgg tttcctttag ataagactgc attgctttca aatagttagc agccacatat
      901 tggtattttc taggctcctt ttcccagcaa gtgtctgcaa aatcccaatc gataatcttt
      961 gtttttttcg cttctagtaa atatttttat agagtttatt tctttcaggc accgcaatac
     1021 ctagaaaaga aaattgatgg cgcatatagg cttccatgga ccttgctttt ttagagtctt
     1081 ttgctgcttc tagctcctca agtaaatctg ctaaactcat ctaaaactca tcttgcccca
     1141 ccaaatggtg ctgaaaggca tagacagccg cctgggtacg atcgctgact tcaagtttgg
     1201 caagaatgtt ggacacgtgg gtcttgaccg tcttgagaga gataaagagg tcatctgcga
     1261 tgcgctgatt ttcgtagccc ttggcgatga gttggagaac atctcgctca cgcgcagtca
     1321 attcttcatg aagttccata tgattgcggt ggtattcaac cttcttgcta acctcttgct
     1381 caatggccag ctcgccagca gctaccttac tgacggcatg aagcaattca tctgcactag
     1441 aagtcttgag catatagcct ttggcaccag catctaagac tggcatgatt ttttcattgt
     1501 ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg attgctaagg
     1561 tcgcgtcaat cccattcatc tcaggcatga caatatccat gacaatgaca tctggacgca
     1621 gttccaaggc caagtcaatc ccttgagacc cgttggacgc ctcacccaca acttctacat
     1681 cgtcttggag gtcaaagtag cttttcaaac ccaaacggac catttcatgg tcatctacta
     1741 gtaaaatttt catctttact cctttatcat tccttatcta acaggggaat acggatatca
     1801 actgccagcc cttgcttggg agctgtcaag agttgaactg ttccagccat atcttcaacc
     1861 ctctccttga tatttcgcag tccataactc aagtcgtcta agcttcctaa ccggaaacca
     1921 atcccattgt ccaccacctt cagttgcaat tcaacatctg tctgatagag gtagacatct
     1981 aggcaagatg cctgggcatg gcggagggta ttgctaatca actcttgcag gatacggaag
     2041 atatgctcct cgattttctt aggcaatttc gtcatattct gcttgagact aaccctaaga
     2101 tcactcttgt cctcaagctc ttttaagaga atttgaatcc cttctatcaa gctcttctgc
     2161 tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca aatccttctg ggcagtttct
     2221 aaaatagctg tgacactctg caactggatc tgcatctttt ctctatccaa tttcaaagcc
     2281 tgctgactga tacccgataa aatcatgtgg gccgcaaaca actcctgact gactgtatcg
     2341 tgcaaatccc gagcaattcg cttccgttct ttctcgatga tttcctcttc ctgagcaagg
     2401 ctatgatttt cagctttttg aagagcttct gtcaaaaggt taagtttacc tgataaggac
     2461 ttgaaactgg catccaaatc tggatctgca acctgaacca cttcttgccc tgccaataaa
     2521 cgcttgagat tagcctgcat ttttcttaga gaaagctctt cgatccctcg ccaaaacagg
     2581 gctaagagac aggttatgga catgctgaaa accaacaata aaaagacaaa tttttctgtt
     2641 ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag caagctgtgg
     2701 gagaaaaaaa agacaaacag gaaggaggtg agagcaataa tgacataggc ttgttttttc
     2761 atcctctaac cacctccaca tcaccaatca tagtggtcaa gaaaatcttg acactcttgt
     2821 tactcttgag atagtctttt gtttcttgat gatagtgttc attgcggagg gctcgcttgg
     2881 gctggttgaa aaaagtcaaa tccccataga gacagttaac gctgagactg acttccacat
     2941 ctacaggtac gatgattttg gtcgttccta ctatctttct gaggataatg acattgtcat
     3001 gattggttaa gatgaccctc tccagatgaa tagtgtcctt gcccatgaag cgaaagagat
     3061 tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg atgaagattt ccaaaccaac
     3121 gatttttctc cttcttaacc gtcacgacct cttcaaaaac caaattggtc tgctcttttt
     3181 cctggttcat catcgggtaa agaagaaaga ggctatagat aaccgcaaca aaaatagcta
     3241 gaatcacaaa aggattgagc ataacgatga aaaagaagag aatggttgcc gctactaaaa
     3301 gaagattatt tccctcttta ccagtgtagt agcgaatcaa aagcaaaaag aggaatagta
     3361 tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat cagagctcct gtcagaagac
     3421 aggcttcgat aaataaaaag attttaaatt ttctcatagg ttcatcctct cccttctatt
     3481 ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga aaaaaaggcg ctggttacgc
     3541 ctttttcatc tgatcatttg cttcttttaa ttttccataa agaagatagt ctactttttg
     3601 tagatctgct atggtggcac agttaaggga acacataatc aagcgtagat ctgctttcca
     3661 gccttggaca atgccaatca cttcttcaac tgtgtaggtt tcaaccaatt ccagaacggt
     3721 tcgtgacaat cccacagcct tagcaccaaa aaccaagcac ttaatcatat ccagcggatt
     3781 ccgaacccct ccactaacca agagttcgac cttatctttc cattcttggg cattgagaag
     3841 ggcctgcatg gtagactgac cccattgatt gaggtaatca cgctggccac tacgacggtt
     3901 ttcgatatag gcaaagctgg tgccaccacg acccgatagg tccactgtac gaacaccgaa
     3961 ttcataggct ctttcgattg tcttggcatc cattccaaag cccacttcct tgaggacaat
     4021 aggaacggga atttgcttgc tataatctgc tagatgcgat tgccagcttc taaacttcct
     4081 ttctccctcg ggcatgagta attcctgcat gacattgaca tgcacttgca atagaacagg
     4141 attcatctct tctacagtct gaaatcctaa ctcgacaggc ttgtccaatc caatattggt
     4201 tccaaggagg agattgggat gactagactt gacagaaaaa gaatcatccg ttggattttt
     4261 gagagctgcg ctataagaac ccgttacaaa taaaatacca caggtttccg ccacctgagc
     4321 cagcttttga ttgatttctc ttcccttatt acttccacca gtcatggcat tgatataaaa
     4381 aggaaagtcc cactttcgac cagcaaactc tgtcgaaaga tcgatttcat ccagattgta
     4441 aagaggcaag gaagaatgaa tcagctccac ctcatcaaag ctattatagg aacttttctg
     4501 ctcaagggca tagaggatat gctcgtcctt acgatttgtc gtcatgtcct atcctttctt
     4561 gatataagag ctcaatcccc agatcggccc aacgattttt taaggttttg gttgattgcg
     4621 catcaaaact cagggcgatg ccacagtcac caccaccagc accactactc ttggcaacgg
     4681 cctgcaaatc ttgactggct tctttcaact gtctaagcaa aggcgtgtaa atatctgtac
     4741 tcaagccttc taaaagcttg ctggctactt ctacttgctc gataatcttt tctgatttcc
     4801 cctgttccaa ggcttcgacc aaagaaacca ccgtttcttt tgaggaagtt aaaaaatttt
     4861 gattgatatt ttgcttgatt tgctggacca tgtgactcga tacagccact tccttggtcc
     4921 atcccactaa gaaatcacat tctaaagttg gtttcacttg tgaaattgaa aatccccaat
     4981 cacgctccag aactgtcgcc aagttttctt cttctaacca agcagccacc ttctggcgat
     5041 caaatgactg gtagagaacc aaatcctctg ccgcaataca ggcaaggtcg cccatggaac
     5101 cattgtctcc tcgcttaagc aagacagcac tagtcagctt gaacaagaga ttctgatcaa
     5161 ccgaaagatt atagagagcc agtaaagcct tgacaaccaa gacaacgacg ctgccactag
     5221 aacctagacc aaactttttc ccttctcgtt ccattttgcc atagattgct agagaaaaag
     5281 gtcttaaatt ctgaccgcga acagcgagga agtctcccat caaagcaatc gtttcttgaa
     5341 tcaagctgta gtcaggattg ggccttaagt ccactgcgaa atcaaacata tctgaataga
     5401 tacggtagct gtcagaaaaa gcaatctcag ccctcatata gatgggaata tcctttatca
     5461 aagctaactg ccctggctct aaaatagcat attcacctgc caaatagagt tttccgcaag
     5521 ttttaacagc aatcatcttg actcaaatcc tttgtttttg acacaatcaa gcgataacgc
     5581 tgaccgaaaa tttctgacaa atgctccaag tctttctcct gacagaagac cttaacattg
     5641 ggaccagcat ccatggtaaa gtagcaggcc tctcctttct cacgaagctg acgaacaaag
     5701 gccatagcct cataagaggc atccgtcaga taagaaaagg ctggactagc agtctttgtc
     5761 gtagcatgca tagccagggc atttttctcc gttaattctc caatcttggc aaaatcattt
     5821 tccttgagat aaatcagcat atcctgatag tccttctcag actgacgaac ccagtcgtcg
     5881 aaagtcgtcg aggtttccac acaaagtttc atcccgtcac ggctagagat tggttttttc
     5941 ttgtcctcta gcaccaacat aatcatagct agtttcaagt ctgtctctac agggtaaatt
     6001 tctccactat ccttatccca ggctcctagt ggtccataaa aactccgaga agaagagcct
     6061 gaggcaaatt tggcttcctg tgccaactga cttctatcca atccaagttt gaaataagca
     6121 ttacaagcct tgaccagggc ggacaaacca ctagaacttg aggacaggcc cgctgccgta
     6181 ggcatattgt tttgagtatc gatacggaca aagccctcac cagctggacg ataacggtca
     6241 ataatcttac tcatcttggc atgctcgacc tcattttgta gctgaccatt gatgtaaaat
     6301 tcgtcagctg ttacattggc tggtaaaggc gacaaggtcg tctctgtata catattttcc
     6361 aaagttagag aaatactgct agtagcaggc accatctctt tttctttttt ctttccccaa
     6421 tatttgataa tagcaatatt tgcgtaggaa cgtactgtta caggctctct atccatgtct
     6481 gaacagctcc tttctcttct aatctttctg ctagttcttg tgcgtgtgtc aaattggtta
     6541 ccaaggctat gatacaacct cctagcccac caccgctcat cttggcaccc agagcaccat
     6601 ggctaagagt cgtttcaacc aaaaagtctg cctcagggct actgactcca atttctttta
     6661 aatgtaaatg cgcttgactg aggatttgtc ccagtccttc agcatctttt tgtgaaatcg
     6721 caacttctgc ttgctgggtt aattctccca aggcatgcaa aaacggtagg gcatccttgc
     6781 ccttattttg aaccacttgg atggcttcac gagtatgacc ataaacaccc gtatcggcaa
     6841 tcaccaaata ggcggataaa tccatctcaa gttctgtaaa tcctacgttc ttgataaagc
     6901 gaataggttg gtcactgaga caggtcttag catccaaacc actaggattc atatgggcaa
     6961 tcatctcagc tcgattgacc aagatttcta gtacatcatg aggcagatca gcctgatagt
     7021 agtcaaatac cgcacgaatg gccgctatgc tgatagccgc tgacgaaccc atcccccgtt
     7081 tctcagggat agccgagtca atctcacaac gaacgcaggc ttctgtgata tccaaatact
     7141 ccagtgaggc ataaaccgcc atggacaagg tatcctcctc ataaaggcgc caaggactct
     7201 ctgcagaaac taccttacag gtcacctcca cctccaaaag aggcagggaa atggcaggat
     7261 aaccgtaaac gaccgcatgt tcccctatta aaattatctt actatgtgcc tgaccgacac
     7321 caactttttt tgtcattttt tccttttact agacgaaaaa acgtcttatt tttcatacaa
     7381 gtattaattc tttcctatct attttattat attttcacaa aaaaagcgat tgtttccatt
     7441 cacaatcgct tctttcatta ttgaacccat tcgccattat agttgacaga atagccatct
     7501 acggtcgtat tcactgccaa ggcacctgag ctataagcgt agtaccatct gccattgacc
     7561 tggaaccaac ctgtcgtcat agaacgatga aagaaactcc ataccattaa gtaaagagga
     7621 aagtcgtgag ggagcatgcg ccattgacaa cctgttttag tgacgtacaa agtctcatta
     7681 acaagtactc gtttcggcca tttataggtg cggtgtttgg agaaataggg ttcaatctta
     7741 gcccattctt gatcatttaa atcagtatca tatgctttgc gtgtcataac tctagtttaa
     7801 catttttttg tgaatagagg ttctaaataa tcgaccacga aaatttctta agtgaaaaac
     7861 gccttatgaa gtatgctacg ggaaagttat gcacttaatt tgacaatt
//
LOCUS       DAVHIU010000084         7785 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVHIU010000084 DAVHIU010000000
VERSION     DAVHIU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.086"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            37..726
                     /gene="coaB"
                     /locus_tag="VVN06_001555"
     CDS             37..726
                     /gene="coaB"
                     /locus_tag="VVN06_001555"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU5563058.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISTDQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            738..1289
                     /gene="coaC"
                     /locus_tag="VVN06_001556"
     CDS             738..1289
                     /gene="coaC"
                     /locus_tag="VVN06_001556"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU5563059.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLITPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1273..1836
                     /locus_tag="VVN06_001557"
     CDS             1273..1836
                     /locus_tag="VVN06_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5563060.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2132..2659
                     /locus_tag="VVN06_001558"
     CDS             2132..2659
                     /locus_tag="VVN06_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5563061.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2671..3186
                     /locus_tag="VVN06_001559"
     CDS             2671..3186
                     /locus_tag="VVN06_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU5563062.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3183..3650)
                     /locus_tag="VVN06_001560"
     CDS             complement(3183..3650)
                     /locus_tag="VVN06_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU5563063.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWIQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKEIF"
     gene            complement(3723..4211)
                     /locus_tag="VVN06_001561"
     CDS             complement(3723..4211)
                     /locus_tag="VVN06_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563064.1"
                     /translation="MVITVKQMETPEEIEGKSLVHWKAWRESYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALDHFSEIFLWVLKDNKRAIAFYQKMGYTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(4177..4740)
                     /locus_tag="VVN06_001562"
     CDS             complement(4177..4740)
                     /locus_tag="VVN06_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563065.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNFGMMKLAEKLRMKKEAHIPKVRYYQGVY
                     YDSIKYGILREEWEEANGHHCQTDGNS"
     gene            complement(4750..6738)
                     /gene="uvrB"
                     /locus_tag="VVN06_001563"
     CDS             complement(4750..6738)
                     /gene="uvrB"
                     /locus_tag="VVN06_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU5563066.1"
                     /translation="MINRITDNKFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVSKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6815..7768)
                     /locus_tag="VVN06_001564"
     CDS             complement(6815..7768)
                     /locus_tag="VVN06_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5563067.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVALGQYRLDSIHLDTYSLAF
                     TLFTIPFWILQGTAEELVTRAWLIPQLAKRTNLKVAIIISSSLFTLLHLGNPGITFLS
                     AIDLFLFGVAMSLYLLKTDTIWGIGGIHGAWNFAQGNLFGVLVSGQSSGTSIMKFTPQ
                     GNQDWLSGGSFGIEGSIVSSIILFLLILYLAYQLKKENERM"
BASE COUNT         2313 a         1738 c         1388 g         2346 t
ORIGIN      
        1 ttttacttac cgcttatttt tgtgtacaat agtgctatga aaattttagt tacatcgggc
       61 ggtaccagtg aagctatcga tagcgtccgc tctatcacta accattctac aggtcacttg
      121 gggaaaatta tcacagagac tttgcttttt gcagggcatg aagtttgttt aattacgaca
      181 aaacgagctc tgaagccaga gcctcatcct aacctaagta ttcgagaaat taccaatacc
      241 aaggaccttc taatagaaat gcaagaacgt gttcaggatt atcaggtctt gatccactca
      301 atggctgttt ctgactacac tcctgtttat atgacagggc ttgaggaagt tcaggctagc
      361 tccaatctaa aagaattttt aagcaagcag aatcatcagg ccaagatttc ttcaactgat
      421 gaggttcagg ttttgttcct taaaaagaca cccaaaatca tatccctagt caaggaatgg
      481 aatcctacta ttcatctgat tggtttcaaa ctgctggttg atgttaccga agatcatctg
      541 gttgacattg cacgaaaaag tcttatcaag aatcaagcag atttaatcat cgcgaatgac
      601 ctgactcaaa tttcaacaga tcagcaccga gctatatttg ttgagaaaaa tcagcttcaa
      661 acagtccaga ctaaagaaga aattgcagaa ctcctccttg aaaaaattca agcctatcat
      721 tcttagaaag gaaaactatg gcaaacattc tcttggctgt aacgggttca atcgcctctt
      781 ataagtcggc agatttagtc agttctctaa aaaaacaagg ccatcaagtc actgtcttaa
      841 tgactcaggc tgctacagag tttatccaac ctttgacact acaggtactc tcacagaatc
      901 ctgtccactt ggatgtcatg aaggaaccct atcctgatca ggtcaatcat atcgaacttg
      961 gaaaaaaagc agatttattt atcgtggtac ctgcaactgc taacactatt gcaaaactag
     1021 ctcacggatt tgcggacaac atggtaacca gtacagctct agccctacca agtcatattc
     1081 ccaaactaat aactcctgct atgaatacaa aaatgtatga ccatccagta actcagaata
     1141 atctgaaaac attagaaacc tacggctatc agctgattgc tcctaaggaa tccctactag
     1201 cttgtggaga ccacggacga ggagctttag ctgacctcac aattatttta gaaagaataa
     1261 aggaaactat cgatgaaaaa acgctctaat attgcaccca ttgctatctt ttttgctacc
     1321 atgctcgtga tacactttct gagctcactt atctttaacc tttttccatt tccaatcaaa
     1381 ccgaccattg ttcatattcc tgtcattatt gccagcatta tttatggtcc acgagttggg
     1441 gttacacttg gatttttgat gggattactt agcttgacgg ttaacacgat tacgattcta
     1501 ccgacaagct acctcttctc tcccttcgta ccaaacggaa acatctactc agctatcatt
     1561 gccattgtcc cacgtatttt gattggttta actccttact tagtctataa actgatgaaa
     1621 aacaagactg gtctgatttt agctggggcc cttggttcct tgacaaatac tatctttgtc
     1681 cttggaggaa tcttcttcct atttggaaat gtttataatg gaaatatcca acttcttctg
     1741 gcaaccgtta tctcaacaaa ttcaattgct gaattggtca tttctgcaat tctaacccta
     1801 gccattgttc cacgactaca aaccttgaaa aaataaaaac aatctccgct ttcaaacctg
     1861 aaggtggaga ttttgctcta tacagtttct ttttaatgaa atctttacta tagtaaaacg
     1921 aaataagaac aggacaaatt gaacaggaca aatcgatcag gacagccaaa tcgatttcta
     1981 acaatgtttt agaagtagag gtgaactatt ctagtttcaa tctactataa cttttaacta
     2041 aaaaatgatc tataaaccca agtaaattct tgctttattg tctggtttat tgtaccatac
     2101 tagtgtatat gcagttaaaa aggagattct tgtgaataca cggaaaaaga cacaatttat
     2161 gacaatgaca gcccttttaa cggctattgc gattttgatt ccaattgtta tgcctttcaa
     2221 gattgtcatt ccacctgctt cctatacttt ggggagccac atcgctattt ttatagccat
     2281 gttcttgtcg cccttgatgg cagtttttgt catcctagcc tctagttttg gatttttgat
     2341 ggctggctat cccatggtta tcgtttttag ggctttttcc catatatctt ttggtgcttt
     2401 aggagctctt tacctacaaa aattccccga taccctagat aaaccaaaat cttcctgggt
     2461 tttcaacttt gttttggctg ttgttcatgc ccttgctgaa gtattagcct gtgtcctttt
     2521 ttacgcaact tcgggtacca atgtagaaaa catgttctat gttctatttg tactagttgg
     2581 atttggcaca attatccata gtatggtaga ctatacatta gcactagctg tctataaagt
     2641 gcttcgaaaa cgccgttaaa aggaaaagct atgacaaaag atcgcaaaca agccctgctc
     2701 caactcttga aagaagctcc taaagccctt aatggccaaa gtttggcgga acattttcat
     2761 gtcacacgtc aggtcattgt gcaggacatt gcaattttga gagccgatgg cgctcctatc
     2821 ctatccacca atcgtggcta catctataag caaattgata ccaatcctta tgttcacaaa
     2881 cttttcaaag tgaaacatga agttgaagaa atcggtcagg aactccttgc tattgtcgat
     2941 aatggcgggc atgtccagaa taccttgatt gaccatcccg tttatggaga aattgaaacc
     3001 ttgcttaaac tgtcttgtcg cagagatgtg caacattttc tagaacaagt cgagcattct
     3061 gactttagac ctctatctga attgacagat ggcgtccatt accacctagt cgaagccgaa
     3121 acacaacaag acctccacta tatcgaggag gccttggatc agctaggtta tttagtaaaa
     3181 gactaaaaaa tttctttctc ccaatcgtct tctgtacggc gagggtagaa aaattcagat
     3241 ttgtccggag cttccatcat ctccatcaaa ggtagcatat cataggccag atccaagttt
     3301 ggaatctggt ctttttgaat ccaagaaacc tctccttcat ctgaagagcg aagggtacca
     3361 gagaactcag tcgccttata acaaacgaca atatagcgcc cacctgtatc tagtggccaa
     3421 tttttaatgc cgacaagttg aggattttgg atagtcaacc ctgtttcttc atagatttca
     3481 cgaatgacag actctgcgaa agattcgcca ttttctacat gacctcctgg aaaggcataa
     3541 cctgaccagc gattggtgtc aggagagcga tactgcatga ctacgcgctg agtttcgagg
     3601 tcttcaatca gacagatatt tgttaaaatt gttaattggg aacgggacat aaatttactc
     3661 gctttctaag ttattaaatt tttagacttg gtaccagtgc catgagcttc tactttttaa
     3721 aattatttag aatagaatac catccgtttt tcctttatag gctttccaag ttcaagtatt
     3781 ttttcttgtc catcaaaagt ataacccatt ttttgataga aagcaatggc gcgcttatta
     3841 tctttcaata cccataagaa aatttcagaa aaatgatcaa gagcagtcaa agctgctttc
     3901 attaactttt gtgcgatgcc ttttccgtaa tagtctttta aaacatataa agcaataatt
     3961 tcaccagctt gaatagtctc atcacgaaag ttgccataac tgataaaacc aactaccttc
     4021 aggccatcca tcgcaatcaa tgtattttct ggatactttt gactaaagag tcgacacctt
     4081 tctaatgtca tcgtctcttg aaattctgca ggcaaaaggt cgtcataaga ttctctccac
     4141 gctttccagt gaacaaggga tttaccttct atctcttcag gagtttccat ctgtttgaca
     4201 gtgatgacca tttgcttcct cccattcttc tctcaaaatg ccgtatttaa tactatcata
     4261 ataaacacct tgataataac gaacttttgg aatatgagct tcttttttca ttcttaattt
     4321 ttcagccaat ttcatcatac caaaatttcc tgaccaagtt gtcaaaccca gatgctccaa
     4381 ctccaagtaa tcctgaaacg tcctatctat ccactgcaac atagcaactt tcccaatacc
     4441 agtgttccag aattttttat cataaatacc aattcccaat tccatccatc ttgtttgttt
     4501 acatacccaa taacgcgaaa cagtcccaac tagtttatca tcaacaaaaa taccaaggcg
     4561 atttgagttg cttaaaatgg attctgattt ttgtagttcg aattctttaa aatttgaaaa
     4621 atactgataa tcatcataat agggagcatc atactgtttc caaactggat taagttgtga
     4681 ataagccatt tcccataaag acatcaaatc ttctttcttt aactttctta aataaatcat
     4741 actattcccc taatccaacg ccttcacttc cagcatcata tcacgaatct gagctgctag
     4801 ttcaaagtca agcacttcga cggcttcttg catttgtttt tcgagtttct taacaagttc
     4861 tttgcgctct tgtttgttga ggctattgat atcaacttcc ttgtcttctt ccttagaaac
     4921 tgccttggtc acagcaatca ggtcacggat ttctttcttg attgtctgtg gtacgatacc
     4981 atgctcttca ttataggcca tctggatttt gcgacggcgg gcagtttcat cgatagcacg
     5041 ttgcatagac tgggtaaccg tgtccgcata catgataaca tgaccttcac tgttacgggc
     5101 ggcacgtcca atggtctgga tgagtccacg ttcgttacga aggaaacctt ccttgtcagc
     5161 atcaagaatc gctacgaggc tcacttcagg aacgtcaatc ccttcacgaa ggaggttgat
     5221 tccgaccaag acatcaaaga cacccaagcg caggtcacgg ataatctcag tccgctccaa
     5281 tgtcttgata tccgagtgca tgtacttgac cttgataccc atttccttga agtagtcggt
     5341 caagtcttct gccattttct tggtcaacgt ggtgataaag gttcgttcgt tcttctcaac
     5401 acgagcatta atttcaccta agaggtcatc aatctgtccc atagtcggac ggacttccac
     5461 ctctggatcc aagagtcccg ttggacgaat gatttgctca atcactgtct cagtctgttc
     5521 attttcatag tcaccaggtg tcgctgaaac gtaaacaatc tggtgcacat ggctttcaaa
     5581 ctcctcccga cggagaggac gattgtccag agcagacggc aaacggaaac cgtaatttac
     5641 taacatttcc ttacgagaac ggtctccatt gtacatgccc ttgatttgtc ccatggtcat
     5701 gtggctctcg tcaatcatga ttaagaaatc atctggaaag aaatcaagaa gtgtgtaagg
     5761 aggctctcct tcgctccgtc catccatgtg acgtgaatag ttctcaaccc cgttggtata
     5821 gcccatctca cgcagcattt cgatatcgta ctctgtccgt tgtttcaagc gctgggcttc
     5881 aagcagtttg ccttcctttt caaagacagc tagttgctct tctaattctg cttgaatctt
     5941 ggcaatagca acttccatgt ggtcgtcatt ggtcacaaag tgagtcgcag ggaaaatcgc
     6001 taaatgatcc acttctccaa ggacttgacc tgtcaaagct tcaacctcac gaatacggtc
     6061 aatttcgtct ccaaaaaatt ctactcgaaa agcgtgttca tcacgggaag cagggaaaat
     6121 ctccaccaca tccccacgaa cgcgaaatct tccccgttgg aaatcaatat catttcgctc
     6181 aaattggata tccaccaagt cattcaagag tttatcacgg gaaatttcaa gacctggacg
     6241 gagactaacg acactatcag cgtattcctt gggcgaaccc agaccataga tacaagagac
     6301 cgaggccacg acaataacat cattgcgctc caatagggct gaagtcgctg agtgacgaag
     6361 cttgtcaatc tcgtcattga cagaactatc cttctcaata taggtatcac tagaagggac
     6421 ataggcttct ggctggtaat agtcatagta ggatacgaag tactcaactg cattttcagg
     6481 gaaaaattcc ttgaactccc catagagctg accagccaga gttttattgt gggcaatgac
     6541 tagagtgggt ttattgactt tggaaatgac ctgactcatg gtataggtct tccctgttcc
     6601 ggtcgccccc atcagaatct gagctttttc tccaccctcg atattatcta ccaactgctc
     6661 gatagcttgg ggttgatctc ctgatggttg atattttgat actagtttaa atttattatc
     6721 tgtaatgcgg ttaatcataa gaatcctctc tccatgtgct attcctattt tagcatactt
     6781 gtatcatttt cacgaagaaa aggacgaacc gaagtcacat cctttcattt tctttcttta
     6841 attggtaagc gagataaaga atcagcagaa acaaaataat actggacacg atggaaccct
     6901 caataccaaa agacccacca gatagccagt cttgattgcc ttgtggagta aatttcatga
     6961 ttgaggttcc tgaagactga ccactaacta atactccaaa gaggttgccc tgtgcgaaat
     7021 tccaagctcc atgaatacca cctatacccc atatagtatc tgttttaaga agatataggg
     7081 acatggcaac cccaaataaa aagagatcaa ttgcagataa gaaagttatg cctggatttc
     7141 ctaaatgaag taaggtaaag agactactag atatgataat agcgacttta agattggttc
     7201 tcttagccaa ttgtggaatc agccaagcac gagtaaccaa ttcttctgcc gttccttgta
     7261 aaatccaaaa aggtattgta aataaagtaa aagctagcga ataagtatcc aagtgaatgg
     7321 agtccaaacg atattgccct aaggccacca aacctaacaa ggtcagaaga aaaagtgcca
     7381 gacctagact aaatcctttc agaagattgc tgaggaaatt ctctctataa aatcccaagg
     7441 ttcgaatagg tcttttctca acttttcttg tccatctgaa cacagtgtta agaataaaac
     7501 caaaggccag taatcctaaa attaaacgaa agtcacttgt tttatccgtc aagctctgat
     7561 gcaaggtctg caagtagctt gcaagatttt gaccagcctc tccaaaattt ctagcaagtc
     7621 cgatgagagc taacaagcca ataccataca aagtaaaagc cacaaactct cctatgaaga
     7681 caaaaacaaa ggctaacaag gggcttgtgt aaatgtttaa actcattttt gaagcttgga
     7741 agtctttaaa tattcttttg ttcttcatat ccccgtcctc ttttc
//
LOCUS       DAVHIU010000085         7524 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVHIU010000085 DAVHIU010000000
VERSION     DAVHIU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.087"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(209..1177)
                     /locus_tag="VVN06_001565"
     CDS             complement(209..1177)
                     /locus_tag="VVN06_001565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5563068.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1322..2137)
                     /locus_tag="VVN06_001566"
     CDS             complement(1322..2137)
                     /locus_tag="VVN06_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU5563069.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     STFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2162..2659)
                     /locus_tag="VVN06_001567"
     CDS             complement(2162..2659)
                     /locus_tag="VVN06_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5563070.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2732..4093)
                     /gene="radA"
                     /locus_tag="VVN06_001568"
     CDS             complement(2732..4093)
                     /gene="radA"
                     /locus_tag="VVN06_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5563071.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINETAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4107..4622)
                     /locus_tag="VVN06_001569"
     CDS             complement(4107..4622)
                     /locus_tag="VVN06_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5563072.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4624..5067)
                     /locus_tag="VVN06_001570"
     CDS             complement(4624..5067)
                     /locus_tag="VVN06_001570"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU5563073.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5124..5221)
                     /gene="ffs"
                     /locus_tag="VVN06_001571"
     ncRNA           complement(5124..5221)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVN06_001571"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5254..5721)
                     /locus_tag="VVN06_001572"
     CDS             complement(5254..5721)
                     /locus_tag="VVN06_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5563074.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSKESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5922..7208)
                     /locus_tag="VVN06_001573"
     CDS             complement(5922..7208)
                     /locus_tag="VVN06_001573"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5563075.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7439..>7524)
                     /locus_tag="VVN06_001574"
     CDS             complement(7439..>7524)
                     /locus_tag="VVN06_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU5563076.1"
                     /translation="DFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         2365 a         1857 c         1237 g         2065 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtacagtca tatttctttt ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatctattt attcggaaaa aagacgaaaa
      181 aacctgagaa tcatctcagg cttggtcatt aaattttttt ctcaatatcg aaaagtggag
      241 aaagtggtcg tttttcatga atacgtacga tagcatcccc taggagatga gcgattgaaa
      301 tctgctcaat cttatcaatc aaacgctctt ctggcagata gatggtatcc aaaacaacca
      361 atttcttaat agctgatttt tggatattgt ccgtagcagg accagaaaga actgggtgcg
      421 tacagcttgc atagacttca acagcaccag cttccgcaag agcatctgcc gcatgacaaa
      481 tcgttccagc ggtatcaatc atatcatcaa tcaagataca agtcttgcct tcaaccttac
      541 cgatgatgtt cataacttca ctagtattca tcttatcaac gctgcgacgt ttatcaataa
      601 tagcgataga tgttttcaaa aattctgcca acttacgagc acgagtcacc cctccatggt
      661 ccgggctgac aaccacatag tcagaaccaa ccataccacg acgctcaaaa taatctgcaa
      721 tcagaggagc acccatcaaa tgatccacag gaatatcaaa gaatccttga atttgcgcag
      781 catgcaagtc gatggtcaat aaacgatcca ctccagctac ttcaagcata tttgcgacaa
      841 gttttgaagt gattggctca cgcgctctcg cctttctatc ctgacgtgca tacccatagt
      901 aaggcatgac aacattgaca gattctgcac tcgcacgctt caaagcatct accataatca
      961 aaatttcaag cagattgtca tttacaggcg aactagttga ttgtaggata aagacgtgtt
     1021 tcccacggat tgattcttca atgttgacct gaatctctcc atctgaaaat tggcgaacac
     1081 ttgatttccc caactctatc ccaatctcct gcgccacacg ttctgccaat tctttattag
     1141 aagaaagggc aaacagcttt aaatcagaaa aagacatgat ttcctccggt atatatgtat
     1201 aacttgtgcc tttcacaaga ttttccatct accattgtag cgctttttgc actatttttc
     1261 aatcaaaaat aaaagaaggg caccatattt gtacccttgc atcattcttt tgaaaaatat
     1321 tctaggtcat caactcattg tgtttctcaa caaagcaata agcatgataa aaaccataga
     1381 gagcaatagc cgtaaccact ggaatcgcta aaggcaactc tgtttccaac tccacaaaag
     1441 gagagttaaa caagaagtga gttcccaagg ctaaacctag aaaaataagg ccctgtttct
     1501 tgccaacctt ctgtccttta taggctctgt aaagcaagta aacacctact acagctagac
     1561 ctgaaaaagt ccagtgagag gcaattcctg agatgatacg ctctaaaatt cgcgaaatag
     1621 taaagtcaaa gccctctggc aaatccgtac gaatataacc aatatcctca atcatttgga
     1681 atcccaaacc ggaagcaatt ccaagtaaaa acaaagattt taattttcgc acaggaatca
     1741 aagccaaaac aaaaacaagt ggcaataatt tcaagggttc ttctaccaaa ggagccacaa
     1801 tagcactttc aaaggcattt aaaaatgtac tatctgggaa aagaaccccc agtaaatcat
     1861 ggatataagt attagcaaaa ctagacaacc agcctgaaag gaacatccct cccaataaag
     1921 acagaatcaa aaccttcttt ggcaattccc attttttccc aatacggaag agaaaataaa
     1981 gagccggaat catgtaaaag agagctagaa agatagaaac tcccattagt ccatattccg
     2041 cacctgacct cgaaccgtcc gtatagtaga tggtttcata ctgtaaacca atacatagca
     2101 ataaaataaa aataaataaa atactgctct tcttcataca ctttctttct aaatgaagta
     2161 tttataattc tacgactgtc atacttcctg tatcaacatt gtaaatagca ccagagataa
     2221 tgacatcgtc tggtattagg ggagactcga taagcagttg catatcctcg cgtacactct
     2281 cttctatatc ttggaagggc aagaagtcct ggtctgacac atctacacct aattcctctt
     2341 ttaaatactc ctgaaaaggt tcattttcaa aggtctgagc accacagtct gtatggtgca
     2401 ataccacaat ctctcttgtc cccatttgtt gctgggaaat aactagcgaa cgaatcatgt
     2461 cttcagtcac tcgaccacct gcattccgca aaatatgagc atccccaagt gccaagccca
     2521 gagcttgcgc aacgtgcaaa cgtgagtcca tacaggtcac aatggctact ctggttttag
     2581 gtttaagtgg cagatttaac tgcccatgta gggcaacata agcctgattg gcttgcataa
     2641 actgttcaaa atacgacacg attccctcct tgaaaatttg atagtcaaat atttctccta
     2701 tcttatcatt tttaagagaa tttgtcacgg attatgcaaa gacctttttc aaaacttcct
     2761 gaatcgttgt cacgccaatg acctgaattt ccttaggcag agtgattcct gtcaaggaat
     2821 tctgaggtac ataaatctta gtaaagccca gtttagcagt ttcgttgatg cgttgctcaa
     2881 tacgattcac gcgccgaatc tctcctgtca agcccagttc tccgacaaaa cattcctgag
     2941 gattagttgg cttgtctttg tagctcgaag caatagcaac tgcaacagcc aagtcaatgg
     3001 caggttcatc caatttaaca ccaccagcag atttgagata ggcatcctga ttttgcaaga
     3061 gaagccctgc ccgtttttcc aaaacagcca taatcaagct agcacggtta aaatcaagtc
     3121 ctgtcgtagt acgcttggca tttccaaaca tggtcggtgt taccaaagcc tgaacctccg
     3181 ccaaaatcgg acgcgtccct tccatggtta caacgatgga ggaaccagtc gccccatcca
     3241 aacgctcttc taggaaaact tgactcggat tgagtacctc aaccaagccg cccgactgca
     3301 tctcaaaaat cccaatctca ttagtggaac caaaacgatt tttgaccgct ctcaaaatac
     3361 gaaaggtgtg gtgacgctcc ccttcaaagt aaagcaccgt atccaccata tgctccaaca
     3421 tacgaggccc agccaaggtt ccttctttgg tcacatgacc tacgataaag atggcaatgt
     3481 tattggtctt ggccaactgc atgagttcag cggtcacttc acgtacctga gaaacagacc
     3541 cctgcacccc tgaaatctca ggagacatga tggtctggat ggaatcaata atgagaaagt
     3601 ctggctggat acgctccact tctgcacgaa cactctgcat attggtctct gcatagagat
     3661 aaaactcact atcaatatca cctaagcgct ctgcacgtag tttaatctgc tgggcagact
     3721 cctccccact gacatagaga actgtcccca cttgggacaa ctgggttgag acttgtagga
     3781 gaagagttga tttcccaatc ccaggatccc caccgatgag gacgagactt cctggtacca
     3841 ctccgcctcc aagcacacgg ttgaattcct ccatctccgt cttggttcga ttgacattga
     3901 tggaagtcac ctcagctagt ttcatgggct tggttttctc acctgtcaag gacacacgcg
     3961 cattcttgac ctcggcaacc tcaacctctt ccacaaaaga agaccaagac ccacagttgg
     4021 ggcaacgtcc cagatattta ggggaattat acccacaatt ttgacataca aatgtcgctt
     4081 ttttctttgc gatgacaaac ctctttctat atctctaact cacactcaat cacttggcaa
     4141 aaatcaatct tctcatttgg cacaaactgg cgcatgagca ttcgatgagc aacaactacc
     4201 acagtctgat gttctcgata cttagacata cattctagaa accgagactt catttccgta
     4261 gctgtctcat attgaatagg actattagga agcaactccc ccttgttttc taaaaacagt
     4321 cttctagctg tttcaaagtt ttctattcct gttttataga cctgccattc atgtaataaa
     4381 ggctctactc ttaaaggaag acccgtagca cagaccacat acgaagccgt ttctaaagct
     4441 cttgtgactg cagaagatac gattatttca gctgacgaga gtaaaggatt tttgctcaat
     4501 ttctggactt gctgccgtcc catctcagac aagggtgcca aatctatccc aaatcctata
     4561 taagaacgct cctctaactc acggtaatct ggctccccat gacgtacaaa gataatcttc
     4621 attctagtgc cctgtcgatc caaatccacc agttcgaacg ccatcagctg catctccatc
     4681 tgcaattaag aaagtagcaa aaacagcctg gacaatacgt tccccaactt caagaacaac
     4741 ctcttggtct gtgatattct tcatctgcgc aaaaatatgc ccttcatttc caggatttcc
     4801 ataataatcc ccatcaatga ccccaactga gttaattaaa accaagccct tcttacgagg
     4861 atttgaagaa cgatcataga ggtagagaac ctcagtcggc tgcatataag ccttaacccc
     4921 tgtcggaacc aagacaatct ctcctggcgc aacaactgta cgcacagcaa cctttaagtc
     4981 gtaaccagcc gcatgcgctg tctcacgctt gggcaataaa ttttcatctg taaaactcga
     5041 aaccaattca aaaccacgaa ttttcataat tttctctttt ctattatcat ttattctaga
     5101 ttattctatc ttatttattc ggaaaaagca cgaaaaaaag agcacacaat tcaaatcgct
     5161 tagggctgct ggattcctcc cctgacccgc ttcacgcaga actgttgctc cactatttat
     5221 tatatcacat tcctattcat tttaaaagca aaattatttt ttccgtctat ttctaaaaaa
     5281 atcctgcatg atagctgcgc attcatcttc caaaattcct atttcaacct ccacacgatg
     5341 attgagacgc tcatctgtca agatatcgta caaactccca gcagcgccaa atttctggtt
     5401 tttagcccca tagaccacat ttggaatgcg ggcaagccca atcgccccac tacacatgac
     5461 acaaggttca atggtcacaa aaagtgtgca atccagcaag cgccagctct ccttactcaa
     5521 gttcgcatcc tctatagcca taatttccgc atgcataacc gctcgctgta attcctcacg
     5581 cgcattatgc ccacgaccaa tgatttcccc atctttgaca atcacacaac caattggaat
     5641 ttcatcgtgt tcaagagcaa tctcagcctc tctcaaagcc tccctcataa agacttcttt
     5701 ttcttcaagc gtataataca tctcttttct cttttcctac ttatcgattt tattattata
     5761 tcatgaatcc caacacaaaa gaagccaccg aatgcggtga ctctataggg agattattat
     5821 gaaaaagaaa agtttaggat atttgttaca acaagttagg aggtcttctt gtaaccgtct
     5881 atagtatacc cgacctatct taaacaaatc ttaaaaatct cttaggacca aacactttct
     5941 aaaatatttg tttgttcacg accaggacct actgagaaag tagaaatacg aacgccaacc
     6001 aattcactca cacgacgaac atagttacgc gcattctcag gaagatcttc caaattgcga
     6061 actccggtaa tatcttctga ccaacctggc aactcttcat agataggctt gcaacgtttc
     6121 aattgctcaa gactagctgg atagtagtca atacgttgac cgtcaagatc ataggccaca
     6181 cagattttca cagtatccaa accgctcaaa acatcaatag agttcaaaga aaggttagta
     6241 ataccagaaa cacgacggct atgacgcatc acaactgagt caaaccaacc tacacgacgt
     6301 ggacgaccag ttgttgtacc atactcatga cccacttcac ggatacgttc tcccacttca
     6361 tcaaacaact cagttgggaa aggaccatct cctacacgac tcgtataagc tttacataca
     6421 cctacaacct tgtcaatctt gcttggaccg acaccagaac caattgtcac accaccagct
     6481 acagggtttg atgacgtaac aaatggataa gtaccttggt cgatatctag cataacacct
     6541 tgtgcacctt caaaaagcac acgtttgcca ttatcaagcg catcattcaa gataacagat
     6601 gtatctatca cgtatttctt gatttgttga ccatattcgt aatattcttc aaaaatatca
     6661 tcgaaaacaa tcgctttact gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg
     6721 ttacgttcta aacgctcacg gaaaatatct ttatctaaaa gatctgcaat acgaattcca
     6781 acacgagcag ccttgtccat ataagctgga ccaattccct taattgtcgt accaatctta
     6841 ttgtcgccct tagcttcttc ttgcaagcga tccaactcga tatgataagg caaaataaca
     6901 tgcgcacgat cagaaatacg caagttatca gttgttacac cttcctcatg aagatagctc
     6961 aactctttta caagagattt aggatttaca accataccat tcccaatgac agatattttt
     7021 tcagggaaga aaatcccaga tggaatcaag tgcaacttaa atttcttacc gtcaatcaca
     7081 atcgtgtgac cagcattatc accaccttgg taacgtgcaa tcacttctgc attcgctgaa
     7141 aggaagtctg taatcttccc tttaccttca tcaccccatt gggtacctac aacaacaact
     7201 gaagtcataa ttttgtctga gccctcaggc tcttcctttc tcacatacat ggcaggactc
     7261 tcacctgcaa ttatatctta caatttatta taataaaaaa tcgccttttt atcaagaaga
     7321 aacaatagaa agatttgcta tttccaacta ttaaaaaatg atttagaaaa attactagct
     7381 atttactatt atctttccat aaaagagtaa attagttcgg aaatttacta aaattacctc
     7441 aacaagaaat aaaaccccga ttcattacca atttttcaag atacaaacga taagcaacac
     7501 gataatggta aacgataaaa tccc
//
LOCUS       DAVHIU010000086         7339 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVHIU010000086 DAVHIU010000000
VERSION     DAVHIU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.088"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            127..1506
                     /gene="glmU"
                     /locus_tag="VVN06_001575"
     CDS             127..1506
                     /gene="glmU"
                     /locus_tag="VVN06_001575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU5563077.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1516..2061
                     /locus_tag="VVN06_001576"
     CDS             1516..2061
                     /locus_tag="VVN06_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5563078.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            2073..2387
                     /locus_tag="VVN06_001577"
     CDS             2073..2387
                     /locus_tag="VVN06_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU5563079.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2404..3096
                     /locus_tag="VVN06_001578"
     CDS             2404..3096
                     /locus_tag="VVN06_001578"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU5563080.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYSYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHFGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3173..3664
                     /gene="rocS"
                     /locus_tag="VVN06_001579"
     CDS             3173..3664
                     /gene="rocS"
                     /locus_tag="VVN06_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU5563081.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3762..4352
                     /locus_tag="VVN06_001580"
     CDS             3762..4352
                     /locus_tag="VVN06_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5563082.1"
                     /translation="MERLEDYIAFDLEFNQHEGQKHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     YGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4361..6124
                     /locus_tag="VVN06_001581"
     CDS             4361..6124
                     /locus_tag="VVN06_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5563083.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(6226..7339)
                     /locus_tag="VVN06_001582"
                     /pseudo
     CDS             complement(6226..7339)
                     /locus_tag="VVN06_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2279 a         1234 c         1600 g         2226 t
ORIGIN      
        1 taaaaatcaa aaaaaatcaa aaaaatgggt taaggaagag actttagagc attttttcat
       61 tcaagagtgc ggaatgattt gaaatatggt ataataaaag ggaatttcta cagaaaagag
      121 aagattatgt caaattttgc cattatttta gcagcgggta aagggactcg catgaaatct
      181 gatttgccaa aagttttgca caaggttgcg ggtatttcta tgttggaaca tgttttccgt
      241 agtgtgggag ctatccaacc tgaaaagaca gtaacagttg taggacacaa ggcagaattg
      301 gttgaggagg tcttgactgg acagacagaa tttgtgactc aatctgaaca gttgggaact
      361 ggtcatgcag ttatgatgac agaacctatc ttagaaggtt tgtcaggaca caccttggtc
      421 attgcaggag atactccttt aatcactggt gaaagcttga aaaacttgat tgatttccat
      481 atcaatcata aaaatgtggc cactatcttg actgctgaaa cggataatcc ttttggctat
      541 ggacgaattg ttcgtaatga caatgctgag gttcttcgta ttgttgagca gaaggatgct
      601 acagattttg aaaagcaaat caaggaaatc aacactggaa catacgtctt tgacaacgag
      661 cgtttgtttg aggctttgaa aaatatcaat accaataacg ctcaaggcga atactatatt
      721 acagacgtca ttggtatttt ccgtgaaact ggtgaaaaag ttggcgctta tactttgaaa
      781 gattttgatg aaagtcttgg ggtaaatgac cgtgtggcgc ttgcgacagc tgagtcagtt
      841 atgcgtcgtc gcatcaatca taaacacatg gtcaacggtg ttagctttgt caatccagaa
      901 gcaacttata tcgatattga tgttgagatt gctccggaag ttcaaatcga agccaatgtt
      961 accttgaaag ggcaaacgaa aattggtgct gagactgttt tgacaaatgg tacttatgta
     1021 gtggacagca ctatcggagc aggagcggtc attaccaatt ctatgattga ggaaagtagt
     1081 gttgcagacg gtgtgacagt cggtccttat gctcacattc gtccaaattc aagtctgggt
     1141 gcccaagttc atattggtaa ctttgttgag gtgaaaggat cttcaatcgg tgagaatacc
     1201 aaggctggtc atttgactta tatcggaaac tgtgaagtgg gaagcaacgt taatttcggt
     1261 gctggaacta ttacagtcaa ctatgacggc aaaaacaaat acaagacagt cattggagac
     1321 aatgtctttg ttggttcaaa ttcaaccatt attgcaccag tagaacttgg tgacaattcc
     1381 ctcgttggtg ctggttcaac tattactaaa gacgtgccag cagatgctat tgctattggt
     1441 cgcggtcgtc agatcaataa agacgaatat gcaacacgtc ttcctcatca tcctaagaac
     1501 cagtaggagc ctatcatgga gtttgaagaa aaaacgctta gccgaaaaga aatctatcaa
     1561 ggaccaatat ttaaactggt ccaagatcag gttgaattac cagaaggcaa gggaactgcc
     1621 caacgggatt tgattttcca caatggggct gtctgtgttt tagcagtaac ggatgaacaa
     1681 aaacttatct tggtcaagca gtaccgcaaa gctatcgagg cagtctctta cgaaattcca
     1741 gccggaaaat tggaagtagg agaaaacaca gcccctgtgg cagctgccct tcgtgaatta
     1801 gaggaagaaa cagcctatac agggaaatta gaactcttgt acgattttta ttcagctatt
     1861 ggcttttgta atgagaagtt aaaactatat ttagcaagcg atttaacaaa agtggaaaat
     1921 ccgcgtccgc aggatgagga tgaaaccttg gaagtccttg aagtgagctt agaagaagcg
     1981 aaagaattaa tccaatcagg tcatatttgt gatgccaaga caattatggc tgttcagtat
     2041 tgggagttgc agaaaaaata gaggaggtca gtatgggtaa atctttatta acggatgaaa
     2101 tgattgaaag agctaataga ggcgaaaaaa tttcaggtcc tcctttgcta gatgataatg
     2161 aggaaactaa gattttacca acctcttctt cccgttttgg ttatgccaat cctaaggatc
     2221 atggttttag ccaggaaacc ttgaagattc aggtcgaacc atctattcat aaaagccgtc
     2281 gtattgaaaa taccaagaga aatgtcttca attctaagtt gaataaaatc ttatttgcgg
     2341 tcatttttct cttgattttg cttgttttag caatgaaact tttgtaatag aaaaggaatt
     2401 gaaatgaaaa taggaattat tgctgctatg ccagaagaac tggcttatct ggtccagcat
     2461 ttagataatg cccaggagca agttgttttg gggaatacct atcatacagg aaccattgct
     2521 tctcatgaag tcgttcttgt agaaagtgga attggtaagg tcatgtctgc tatgagtgtg
     2581 gcgattttgg ctgatcattt ccaggtggat gcccttatta atacgggttc agctggggca
     2641 gtagcagaag gtatcgctgt tggggatgtc gtgattgctg acaaattagc ctatcatgac
     2701 gtggatgtca cagcttttgg ctattcttat ggacaaatgg cgcaacaacc gctttatttc
     2761 gaatcagaca aaacctttgt tgctcaaatc caagagagtt tatctcaatt ggaccaaaac
     2821 tggcattttg gtttgattgc tacaggagat agttttgttg caggaaatga caagatagaa
     2881 gcgattaagt cccatttccc agaagtttta gccgtggaga tggagggggc agctattgct
     2941 caagcagcgc atgccctcaa tctcccagtc ttagtcatca gagctatgag tgacaatgcc
     3001 aaccatgaag caaacatctt ttttgatgag tttattatcg aagctggacg tcgctctgcc
     3061 caagtcttgt tgaccttttt gaaggcttta gattaagcgg aaatttgaca gtttttctag
     3121 cttatgataa gatttaagta aagaaaagct agaaaacgtt tcagaggata ttatgagtat
     3181 tgaaatgacc gtcagtgaga ttgcagaggt cttaggatta tctcgccaag caatcaataa
     3241 ccgtgtcaaa gaattaccag aagaagacac agataaaaat gacaaagggg taacagttgt
     3301 taccagaagt ggcttgatta agctagaaga aatctataaa aaaacgattt ttgaagatga
     3361 gcctgtcagt gaagatgtca aacaacgtga actgatggag attctagtgg atgagaagaa
     3421 tgcagaaatt cttcgtcttt atgaacaatt aaaagccaag gatcgtcagt tatcagaaaa
     3481 agacgagcag atgcgtatca aagaccgtca gattgctgag aaagacaaac aattagatca
     3541 gcaacaacaa ttgacccttc aagccatgaa ggatcaagaa aatcttaagc tagagctgga
     3601 ccaagcaaaa gaagaagtcc aatccactaa aaaaggcttt tttgctcgtt tatttggagg
     3661 ataatcaagg agctgtttag gttaaatgct aacctgatgg cttcttttgt ttgtgaatag
     3721 tatagttgat tagaacagag acagtagagg agaggtacag aatggaacga ttagaagatt
     3781 acattgcttt tgacttagaa tttaatcagc acgaaggaca aaaacacttg attcaagtat
     3841 cggcagtgcg ttttagagat ggtcgggaaa tcgatgccta tgattcctat gttcatacca
     3901 gcgtccctct aaaaagcttt atcaatggtt tgaccgggat tacagctgag accttaaaag
     3961 atgctcctcc agttgagaaa gtcttagaag aattccaaga gtttgtgggt tctttgctga
     4021 tgataggcta caatgctgct aaaagtgatt tacctatttt agcagagtat ggtttagact
     4081 atagccaaca gtacaaggtg gatttgtatg atgaagcttt tgaacgtcgg agttcagatt
     4141 tacatggtat tgccaatctc aaattgcaaa ctgttgcctc atttcttgga tttcaaggtc
     4201 agtctcataa tagtttagag gatgctcgga tgacggcgcg tgtttacgaa tccttcttag
     4261 agtcggatca agctagggaa ttgttagaga cagaaagtag cttatcaaac aatccttttg
     4321 gtggcttgga cttgtctcaa ttatttgact aggagtgcct atgaaacctg aaaaacctaa
     4381 aaaattgggt cttaaacaga tttacctcaa tcttgataaa atcctctttc tatttttctt
     4441 gatttttatg atagtggagt atttgtgggt accattcaat tcatggttgg ctagcttctt
     4501 attagatcag acaggttatc tttttatttc ctataacaat gtcttagcca tccttactca
     4561 ttctccattg attagtctag cttttttagc cttgattgta attaaccttc tggttgctta
     4621 ttttcaaata ggactcctct ttattggtgc tcgtcatctc ctctataatg aaaagcgaag
     4681 gctaattgag tatatccgca aggttttcca tgaaagtttt ggatttatga aaaagctaac
     4741 ttgcgctaaa gccttgttta tctttttcta tatagctatg ctttttcctt ttatacggaa
     4801 aatgctgaag atttattatc ttaataaaat cgtcattcca gagtttatcc aagcttattt
     4861 agaagataaa tattggatgt ggtggctgag catcctactt ctatctctta tctttctcta
     4921 tgtctctgtc agattgatgt ttgcccttcc gaagattgta tatgatcagc tgactgttcg
     4981 agaagcagtg atgtttagct tggagaaaac caagaagaga gttacttttt atgcttggaa
     5041 tttattttac atcttgctca aagcacattt attattttat cttcctttga ttcctttatt
     5101 attggctcaa actttggtag atgatatcac tcagagagag tctctgattc ttggtatttt
     5161 gaattttgtc gtgattaaaa atctatatta tatggctctg acttattttt tggttaaatt
     5221 tgtttcattt ttgacaggaa aggagctgga tatgcttcct aggagagaaa aagatcacat
     5281 cgtgcgatgg ggtgtcatga cttgtgccag tcttttcttt gccttagaag gttatattta
     5341 tctggaggct cccatggttc atctacctca acttatttct caccgagggg tttccaatgc
     5401 aaatgggatt caaaatacag tagagtcctt ggaaactaca gcacaactca aaccagactt
     5461 ggtggagacg gacgtgcagg aaacaaaaga tgggcagttt gtcatgatgc atgatgctaa
     5521 cttgaaaaat ctagcaggta tcaataaaag tcctcaagac ttaaacttgg aggagcttaa
     5581 agggattgat atttttgaaa atggctacca gactaaaatt tcaagctttg aagattatct
     5641 cagtcgagcc aacgaacttg gtcaaaaatt actaattgaa attaaaacca gtaaaaaaga
     5701 tagtccagac atgatgaacc gctttttagc ccgttatgct gcaaagctca agatttatgg
     5761 acatcaaatc cagtctttag actaccatgt tgtcgaaaaa gtaagacagt atgatgccga
     5821 actgccagtt tatttcatca tgccctacaa ttctgtcttt cctaaaacaa gagcgacagg
     5881 atatactatg gagtactcaa ccttggatga atattttgta agcaaactat ggacaacgga
     5941 tcagaaactt tatgtctgga ctgtcaatga ttcagaagct atcagcaaat ctcttcactt
     6001 aggagtagat ggagtgatta cagatgattt ggaaaaagtt cagcgggaaa tagaagtagc
     6061 ccaagaagat ccagaatata cggatttgct cttgaaaaaa gcattggaat tctttgaatt
     6121 ttagtaataa aaaagaggaa cggttgttcc tcccatgata ggtaagtatt cttgtgaatg
     6181 cttgcttttt tggctctttg tcaactgtag tgggttgaaa aaaagctaag cttgagaaag
     6241 gacaaatttt gtcctttctt ttttgatatt cagagcgata aaaatccgtt ttttgaagtt
     6301 ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt ttgatgagat tattggtcgc
     6361 ttccagtttg gcattagaat agtgtagttg aagggcgttg acaatctttt ctttatcttt
     6421 gaggaaggtt ttaaagacag tctgaaaaat aggatgaacc tgcttaagat tgtcctcaat
     6481 aagtccgaaa aatttctctg gttccttatt ctggaagtga aaaagcaaga gctgatagag
     6541 ttgatagtgg tgtttcaagt cttccgaata actcaaaagc ttgtttaaaa tctctttatt
     6601 ggttaagtgc atacgaaaag taggacgata aaaacgttta tcactcagtt tacggctatc
     6661 ctgttgaatg agtttccagt agcgcttgat agccttgtat tcatgggatt ttcgatgaaa
     6721 ctgattcatg atttggacac gcacacgact catagcacgg ctaagatgtt gtacaatgtg
     6781 aaagcgatca agaacgattt tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc
     6841 ctgagcctag aaatttgaaa gcgaagctgt ttagccaagt catagtaagg actaaacata
     6901 tccatcgtaa tgattttcac ttgacaacga acggctctat cgtagcgaag aaagtgattt
     6961 cggatgacag cttgtgttct gccttcaaga acagtgataa tattaagatt atcaaaatct
     7021 tgcgcaataa agctcatctc catctcccga ttgaaacagt cactccccgg actgtttcaa
     7081 cgtcccaaga cataatctta ggcagatttc taaaattaca ctcaaagtgg aagtcattga
     7141 gctttcgaat gacagttgaa gttgagatgg aaagctgatg ggcaatatca gtcatagaaa
     7201 tcttttcaat taacttttga gcaatctttt ggttgatgat acgagggatt tggtgatttt
     7261 tctttaccag gggagtctca gcaaccatca tttttgaaca gtgatagcac ttgaaacagc
     7321 gttttctaag gagaattct
//
LOCUS       DAVHIU010000087         7216 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVHIU010000087 DAVHIU010000000
VERSION     DAVHIU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.089"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            31..342
                     /locus_tag="VVN06_001583"
     CDS             31..342
                     /locus_tag="VVN06_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563084.1"
                     /translation="MLSGLIFFLAQGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGV
                     LTIDFLFFVLLAFISSQRFSSLMSIITACHSTFYYMTADYLRENYPDFYDKHISLWEC
                     L"
     gene            361..546
                     /locus_tag="VVN06_001584"
     CDS             361..546
                     /locus_tag="VVN06_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563085.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(813..1256)
                     /locus_tag="VVN06_001585"
     CDS             complement(813..1256)
                     /locus_tag="VVN06_001585"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU5563086.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1286..3508)
                     /locus_tag="VVN06_001586"
     CDS             complement(1286..3508)
                     /locus_tag="VVN06_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5563087.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            4504..5133
                     /locus_tag="VVN06_001587"
     CDS             4504..5133
                     /locus_tag="VVN06_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5563088.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(5256..7148)
                     /gene="pepO"
                     /locus_tag="VVN06_001588"
     CDS             complement(5256..7148)
                     /gene="pepO"
                     /locus_tag="VVN06_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU5563089.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVVAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1941 a         1708 c         1390 g         2177 t
ORIGIN      
        1 attagactcg agacatcttt tgagtggctc ttgctctctg gtttaatttt cttccttgct
       61 caaggactcc tgctatttct cttgatcgtc cgactcaaac atcaattcgc tgagatttat
      121 cctcaaatca ataaaaagat tcgcttctac tatttagggg ttctcaccat tgattttcta
      181 ttttttgttc tcttagcctt cattagttct cagcgttttt catctcttat gtcaattatc
      241 actgcttgcc attctacttt ttattatatg acagctgact acctaagaga aaactatcca
      301 gacttttacg acaaacacat ctctttatgg gagtgtctct aaggaaaagg aggttttagc
      361 atgaaaaaaa tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg
      421 gcatttttga catttaaaag ggggcttact tgggactgga ttttgattta tagcggttgg
      481 ctcattttct ttcatcctgt gctattgacc tatctttcaa accaactttg tgaccacttt
      541 agttaactct attcccagat tagaccgaga ttctggcgtt ttgctttaca aattctccta
      601 tgggatagcc tgatgattct ctccttggtg tctttaagtg atattccact tttccttcag
      661 ggaactctcc tcatcctagg acacctcatc ccttcctatc gcatctgcca aagcctgaaa
      721 agagacttcc cccaagcata tcaagaaccg atttcttttt ggagtatttt atgatagatg
      781 agaaagacca agccgactgg gcttggtctt tcttatctct ttttagtatc taggataatg
      841 gtaacaggtc cgttattaac cagctcaacc tgcatatctg ctccaaagat acctgtctga
      901 acgggcactt cttgcgctaa tttttgattg aaagcatcat agaagtctga tgccatatca
      961 ggtttagctg cccctgtaaa ggctggacga ttgcctttct tagtatccgc aaagagggta
     1021 aactgagaaa tagagaggat ttctccttca atatctttga cagacaggtt catcttgcct
     1081 tctgcgtctg aaaaaatccg catattgacc agttttctca cagcatagtc caaatcttcc
     1141 tcttggtcct ctggtccaac accaaccagc aataaaagtc cctgattgat ttttccctga
     1201 atctggcctt ctatactcac ttgggctttt ttaacccgtt ggataatgat tttcataata
     1261 gcctttctag taagagctag gacaactagc cgttggtccg tttgacagag taaacttctg
     1321 gcacactctt aattttatcg acaaccgtgg tcagtgtaga gaggttggca ataccgaagg
     1381 acacatggat attagcaaac ttcatatcct tggttggttg ggcattgacc gttgaaatat
     1441 tcttggttgt atttgaaaga acttgcagta catcgttcaa cagtcctgta cggttgagac
     1501 cgtagatatc gatatgggcc atatactcct tatttgagct agagtactgg tcttcccatt
     1561 ccacatcaag gagacgttgc tcgtagtttt cttgggcacg cagattcata cagtccacac
     1621 ggtgaatagc cacaccacga cccttggtaa tgtagccaac aatatcgtca ccaggcacgg
     1681 ggttacaaca cttagcaatc cgcactagga gaccagaagc accttcaata accactcccc
     1741 cctcatgctt gaccttgaga gtttctttat tttcaacctt gacctcgcca cctttgacaa
     1801 gctcctctgc ctcagccttg gccttggcac gctcttcctc acggcgttcc ttttcagtca
     1861 gacggttaaa gacggtaatc gcaccgattt ccccaaaacc aatggccgca aagagggagt
     1921 cttctgtctt gtaactggtc ttttgcagaa cttgatccat gtggcgcttg tccataaatt
     1981 tatttgccac atagccattt tcttggaact gagccatcag catctcacga cccttgttga
     2041 cagacaattc cttatcttgg tttttaaaga actggcgaat cttattgcgc gccttgctag
     2101 tcttgaccat attgagccag tcacggctag gtccaaagga gttcgggttg gcgataattt
     2161 caacctgatc ccctgtcttt aacttggttg tcagtggaac catgcggcca ttgaccttgg
     2221 caccagttgc tttttcaccg accttggtat ggatttcgta ggcaaaatca atcggtcctg
     2281 aatctttggg aagggaacgg acagctccat ctggggtaaa aacgtaaatc tcctcagcca
     2341 gatagttttc cttaacagag tccacaaatt ccttagcatc atcagcctgg tcttggagct
     2401 ccatcatctc cttgatccag ttcattccaa tagctgattc cttgctgtta acttgcccct
     2461 ttataccttt cttataagcc cagtgagccg caaccccgta ctcagccacc tcgtgcattt
     2521 ccttggttcg aatctggaat tcaatcggcc cttttggccc ataaacagtc gtatggatag
     2581 actgataacc attggccttg cggttggcaa tatagtcttt gaagcgacct ggcatcggtt
     2641 tccaaaattc atgcacgtaa ccaagcatgg cataaacatc actttgggta tctaaaatac
     2701 aacgaatagc aatcagatca tagatttcct caaaccgttt tctcttgtcc tgcattttgc
     2761 ggaaaattga gtaaatatgc ttgggacgac cataaatctt ccctttcaag tgacgttctg
     2821 tcgtatactc ctctaatttt gtgactacct catccaccaa ggcctcacgc tccctgcgct
     2881 tttccttcat catatgggta atcttgtaaa actccgttgg attgagataa cggaaagaca
     2941 agtcttctaa ttcccatttg acactggaaa tccccaaacg atgggcaagt ggggcataga
     3001 tttccatggt ttctttggaa atacgctcct gcttgtcttt tcgaagatgt ttcagggtcc
     3061 gcatattgtg caagcggtca gacagtttga ccaaaataac gcggatgtcc tcagacatgg
     3121 ccatgagcat cttgcgatga ttttccgcta attgctcctc gattgatttg tactcgacct
     3181 tgccaagctt ggtaactccg tcaacaatca cccgcacatc aggaccaaac tctctttcca
     3241 aatcgtccaa ggtcgcatct gtatcttcca ccacatcatg caagaatcca caagctactg
     3301 ttacagcatc cagctttagc ttagctaaaa tacctgccac ttggataggg tgaatgatat
     3361 aaggctcgcc tgatttgcga tattgaccac tgtggcattc aacagcatag accaaggcct
     3421 tatggacaaa atgaacatcc tcttccgtta aatattcttt ggttaaagcg acaacttctt
     3481 cgcctgttaa attcacttct ttcggcatct ctactctcca attcttccta ccattttatc
     3541 acttttttaa gaatatgaaa actagattgg aacagaataa gaaaaaaata attcaaaatt
     3601 gcttgataat tctgaattat cggcccataa tatactacga agttagattt taaacttagg
     3661 tgatagaagg agagatagaa gaacggaaac catattgtaa cccaaagact ttctgatttc
     3721 cccaattcca ttgaagatac gaaagataaa cggtggaact cgtatcacat acactggtac
     3781 cttgactgga ttttggaatt aatactcaat gaaaatcaaa gagcaaacta ggaaactagc
     3841 cgcaggttgc tcaaagcact gctttgaggt tgtagataag actgacaaag tcagtcacat
     3901 atatacggca aggcgacgtt gacgcggttt gaagagattt tcgaagagta taaaaatcct
     3961 caagatactt tcttctatcc tttagtttat aaggagaata cctatgaaaa aaactgctat
     4021 ttctatcttt gctctcctaa tgttaggagt ttgctgcctg ttcctattca gccagcaaag
     4081 ctataaaaaa cagtcgttca atactatgct aacgaccaga acctgcccag taggataact
     4141 tatagtgaat atagcgacaa acgagaagcc aactacggta gcactctaaa catcacgtct
     4201 atcaaacaag ctaatgacgg agtttatgca acctatgaag ggcaattgac acctttccaa
     4261 tattgataaa ttgataacca gcctgtcttc atctagtcat gctggttttt aagttcattt
     4321 taaatcctta cctattctcc ctaactgtgc tatacttaat ttatactcaa tgaaaatcaa
     4381 agagcaaact agaaagctag ccgcaggctg ttcaaagcac tgctttgagg ttgcagataa
     4441 agttgacgca gtttgaagag attttcgaag agtattagta cattctttga gattggagct
     4501 agtatgaaaa tccataaaac cgtgaatcct gttgcctatg aaaataccta ttatctagaa
     4561 ggcgaaaagc acctcatcgt cgtcgatcct ggtagtcatt gggaagccat tcgtcagaca
     4621 atcgagaaga tcaacaaacc gatctgtgct attctcttga cccacgccca ttatgaccat
     4681 atcatgagtc tggacttggt tcgcgagacg tttggcaatc ctcctgtcta tatcgcagag
     4741 agcgaagcca gctggctcta cactcctgtc gataatctct ccggtctccc tcgccacgat
     4801 gatatggcag atgtggtcac aaaacctgca gaacacacct ttgtctttca cgaagaatac
     4861 caactagagg aatttcgttt taaggttcta ccgaccccag ggcactctat cggtggtgtt
     4921 tccctagtct ttcctgatgc tcacctagtc ttgacgggag atgctctatt ccgcgaaact
     4981 atcggacgga ccgaccttcc gactggtagc atggagcaac tccttcatag tatccagacc
     5041 caactcttca ccctaccaaa ctacgatgtc tatccaggac atggtccagc tactactatc
     5101 gctcacgaaa aggccttcaa tccctttttc tagcaagatg atgacaatcg aaatttaagt
     5161 aaactatcca gcaaatcttt ctattacaaa aggcatccta tcaaggtttt cacacgtgat
     5221 tggatgcctt ttttctgatg actagatttt ttgtattacc aaataatcac gcgctcctct
     5281 ggtgaacgcc acattccgtc tccttctttg acatcatagg ttgtaaagaa atcgtcgaag
     5341 tttggtactt gcacattgac acggagtttg gctggtgcgt gcacatcgac gctagccaaa
     5401 agtttcataa attctggtcg acctttcatg cgccagatgc gaccgaagtt gtagaagaac
     5461 tcttctgctg agaagtctgc ttctctctta gctgcttcaa gcgctgctgc gattcctccc
     5521 aagtcagcca cgttttctga tacagtcaat ttaccgttaa tggttgctcc ataagaatcc
     5581 tgtccatcaa attggtcaat gactttttgt gttttctcct tgaaggcagc atagtcgctc
     5641 tctgtccacc aatccttgag gctaccattt tcgtcaaagg aagccccgtt agtatcaaag
     5701 gcgtgggaaa tttcatgggc aatcactgcc ccaataccac cgtagttagc agaagatgac
     5761 tgatgcaagt catagaaagg cgcctgtaaa atggccgctg gaaagacaat caggttcttc
     5821 tgaggattgt agtaggcatt gaccatatga gcaggcatgc cccattcctt atagtctaca
     5881 ggctggttcc acttactcca actgtgcttg atttccacac gcgcaaaggc tagagcattc
     5941 tcaaaaagac tggcagtttc attcactacc ttatccttgt aacgtgcagg caattcttct
     6001 ggatagccaa tataaggttt gatcacattg agcttcacga tagcctgttt acaggtttct
     6061 ggagtgagcc agtcattctt aagcagacgc tccttataga catcaatcat ggttgccact
     6121 tttttctcca catccgcctt ggcttctgga gagaacttct cacgggcgta ccaaagcccc
     6181 agggcttgct tgaaaggttc ctgtgctaga tgataagctg ctttgacctt atcttttgcc
     6241 tctggaactc cagaaagggc acggctgtag gcaccagaca aaacacggat atcctctgtt
     6301 aaatagctgg ttgaaagatt gacaacactc aaaatcaagg ttgctttaag gagagaccag
     6361 gcttcctcac tgtaaaattg ctctgctgct tgccagaaac gttcctcgtc tacaataacc
     6421 ttgtctggta attgcccaat aactgctttg aagaagtcat ccaaaggtag ggcaggcgcg
     6481 aatttcttga aatcttcgta agaatatgga tgatagagtt tagcatattc tgaactttct
     6541 tcattagaga gcaccactgc cacaactcgg cggtccaatt caagtctttt ttctagcaag
     6601 tcttcaattt cttcatcaga gaaatcataa gccttgagga gatttgcgct gctttctttc
     6661 caaagagtca agagctcttc gcgctgagga tgttcttctg catagtaggt cgtatctggc
     6721 aagattgtgc ttggagcgct agcccataga acattgattc tagcatccat aaagtctggc
     6781 gatacaccaa aaggaaggaa gtttggtttt cctgcaagct caaactctgc tagtttagct
     6841 gtaaaatccg caaaagtttc caattcttgg aattctttaa ggagtggtaa gacaggtgtg
     6901 ataccgtcag cttctctctt gtcaaaatca cgaactaggc ggtggtattt gacaaagttt
     6961 tccaagatag catcctcagg cacttcttca cctgctaacc acttgtctgt tgtcgccagc
     7021 atcaggtctt caatttcctg gtctaaatca acaaaacctc ctgtttgaga cttatctgct
     7081 gggatttcag ctgtctgttg ccattctcca ttgatagcat cataaaaatc atcttgataa
     7141 cgtgtcatct tgttctcgct ttcatttgta tttgcattta tcttaacaaa aatcactagg
     7201 gaatgcaatt aataac
//
LOCUS       DAVHIU010000088         7093 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVHIU010000088 DAVHIU010000000
VERSION     DAVHIU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.090"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(147..1007)
                     /locus_tag="VVN06_001589"
     CDS             complement(147..1007)
                     /locus_tag="VVN06_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU5563090.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(1282..2268)
                     /locus_tag="VVN06_001590"
     CDS             complement(1282..2268)
                     /locus_tag="VVN06_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU5563091.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(2540..4015)
                     /locus_tag="VVN06_001591"
     CDS             complement(2540..4015)
                     /locus_tag="VVN06_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU5563092.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(4273..5196)
                     /locus_tag="VVN06_001592"
     CDS             complement(4273..5196)
                     /locus_tag="VVN06_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5563093.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(5210..6139)
                     /locus_tag="VVN06_001593"
     CDS             complement(5210..6139)
                     /locus_tag="VVN06_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5563094.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(6458..6679)
                     /locus_tag="VVN06_001594"
     CDS             complement(6458..6679)
                     /locus_tag="VVN06_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563095.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(6633..6893)
                     /locus_tag="VVN06_001595"
     CDS             complement(6633..6893)
                     /locus_tag="VVN06_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563096.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         2099 a         1429 c         1372 g         2193 t
ORIGIN      
        1 tttatgttat aataggctcc ataatatcca taggggattt acccactaca aatattatag
       61 agccgatttt cattgagtag caatcctgtc atccttggca ggatttttct atttcaaaaa
      121 agcaagtcga ttgacctgct tctgctttac ttttcttgtg ctaatgtttt aaaatctttg
      181 tctaatatgg gttgcacttc tatttgattg gcgtctagtt ggtggtaaaa agatgggggt
      241 aatacctcta agaattttgc atagtccaag cgagctatgg attcatagtc ttctggttta
      301 gagccgtctc ctgtgatttg aaccaagtca atctcccact ggacttcctt atccaccaat
      361 tgctcaatat aggctgcagg aacaaacggt tctctcggta aaactgtctt gactccttga
      421 gctagatggt aaacgccatg cacttttcct tctccatcct gatagaagga aactctcgca
      481 aagtaggcat ctgtctcttg aaccgcacgc acacgcgctt caaactgagc caagccatct
      541 gcctctaaaa agcttttcaa gttctcacga ctaaagaaaa taggattaaa aattccttgg
      601 caaatcgtaa aacgagctac agggtctgct agataggctt gaatacttgc ttggaaataa
      661 gcttcaaaat caaaaccatc tagcccttca atatcagtcc ctgtataagc aagcaaagca
      721 tctaaaaatg acttgataat ctgccagtct gtctttgtta gtaggtcagg aagatcgaca
      781 cggtaagcct tttgaccatc agcataactg accttaaaga gaaattgtga ttgccccact
      841 atcgcacact caatatactc gagacgatta agaggctgac ggagatagac tacatcatag
      901 ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact tggcattcaa aatctcttgt
      961 tctcctaaaa tgctctgttt atttggaata aaaaatgttt tcgccataac tggactcctt
     1021 tgaaatcgtt tacatacagt ataccttatt tttctgaaag atacaaaaac aaactttata
     1081 ttcttcgaaa atctcttcaa accgcgtcag cttcgccttg ccgtagatat atgtaactga
     1141 cttcgtcagt cttatctaca acctcaaagc agtgctttga gcagcctgcg gctagcttcc
     1201 tagtttgctc tttgattttc attgagtatt agatttttga gtacaaagca tagaaaaaca
     1261 gcccctaagg actgtttgat cttatacagt agctgcctga tccaagcttt caccgatagc
     1321 ggctaggcgc tcaatcactt cagcttgtgt caattcattt tttgaaacat agcggttacg
     1381 tgggtgaaca cggcactcgt gtgagcatcc acgaaggtac ttgtcttcat tttcttctga
     1441 tgtcaagata cgacggttac agaagggatt tccacagttg acataacgtt cacatggtgt
     1501 tccatcaaac cagtctttcc ctacgatggt tgggttgaca tggttgacat caacggcaat
     1561 acgctcgtca aagacgtaca ttttcccatc ccaaagctca ccttgaactt ctgggtcttt
     1621 accgtaagtt gcgattcctc cgtgcaattg gccaacatct ttgtaacctt cacgaaccat
     1681 ccagcctgag aatttctcac agcgaacacc acctgtacag taaaccacga cacgcttgtc
     1741 catgaatttt tctttgttgt cacggaccca ttgtggtaac tcacggaagt tgcgaatatc
     1801 tgggcgaata gccccacgga aatgtcctag gtcgtactca taatcgttac gtgtgtcaag
     1861 gacaacggta tctttatcaa gaagcgcttc tttgaactct tttggagaca agtaagcacc
     1921 tgttgtttca agtgggttga tgtcattgtc aaagtcgttg tcttccaaac caaggtggac
     1981 aatttctttc ttgtagcgaa caaacatctt cttgaaggct tgttcacttt cttcgtcaat
     2041 cttgaaccag agatcttcca ttcctggaag gctgtgaacg tagtccatgt atttttgagt
     2101 tgtttcatag tcacctgaaa ctgttccgtt aattccctcg tcagcgacta ggatacggcc
     2161 tttaaggccg attgatttac agaaagccaa gtggtctgca gcaaattgct ctgcattttc
     2221 aattggagta taaaggtagt aaagtaagac acgaatatct tttgccataa gatttgttct
     2281 cttttctatt cttaaatttt ctaaattttt tatttatcca tactagtata cctgcttgag
     2341 aaaacgaatg caatttattt gactggaaat tgtagaggat tctcgaaatt tcctgaatgg
     2401 ttaaaataag gacaagagaa aacatggata tctatatcct tgtgccaaaa aaaccactgc
     2461 cctccccaga ccaacctgag gaaagcagtg attcttattt taggagttag gaatgaatac
     2521 acgaaatcaa tttagctgat tattttttgt ttttcaagaa ttcatcgtat tgtttttgca
     2581 tttcgttcaa tactttttcg taggcacctt cagatttcaa tttttccatc aattctggaa
     2641 tagctttatc tgggtctaca gtaccagtgt tgatagctgt atcaaattgt tgcattgtgt
     2701 tagcaatagc tgagatttca gatttcacat tgtcagtatt gaagataaat ccaagcgctg
     2761 gagattcttt agcttctgcc aattctttct tagaattttc gatttgttgg tctgtaacgt
     2821 tttcgttgat gtaaaggatc cagttgttac cagtgttcca tccacccatg tgagtgtttc
     2881 ctttgtagcc atcaagaacg cgaacacggt tttctttacc ttcaattttt tcccagttct
     2941 tgccttctgg accgtaaaca agaccgttca agagttctgg attcgtattc aagaggttca
     3001 agatttccat tgatttttct ttgttcttag agttgtttga gatgacaaag ttagcaactt
     3061 gtgttgtttg gtttttcttg atgaagttag taattggttt gatttggata tctttgttgg
     3121 caacacgtga aagcaagctg ttaccgtagt cagctggtcc tactgtttct tcacgaacga
     3181 accaagtatc ttgttgaagg tcaaaggaag tatcgcttgt tgcgacgtct tttggaatgt
     3241 agccagcttc atagaatttg tgaagagtct tcaagtgttc tttgaaacga ggcacttcgt
     3301 aacggtttac aactttagta gtatcgcctt caaggtcgat aacgaatgga agaccgtttg
     3361 ctactgggta gtcaaagtta tcagatggga tgaaaacttt accaatagca aatggtacta
     3421 cgtctggagc tttttcttta atttgtttca agactggctc aagagtttcg taagaagtaa
     3481 cacctgaaat atcgatacca tatttagcaa ggagagttcc gttgaaggca aagttttgag
     3541 atgatgcaac gttggctgca actggaacag cgtaaatctt accatttaca gtattaccct
     3601 tgatgtaagc tgggtcaagt gctttgtaaa ggtctttacc ttcttttttg tacaattctg
     3661 tcaagtcagc gtaagcacct ttttgagcat ttacaatata gttatctgca aaggcaatat
     3721 catagttttc accagatgat gtgataactg acattttctt accatagtca ccccagccaa
     3781 ggtattggat atccaatttg gcaccaactt tttcttcaat gattttgttg gcatttgcta
     3841 acaattcatc caagttgtct ggtttgtcac cgatttggta cattttgata acaggtttgt
     3901 cacctgaatc agcagctttt ttgctgttac ctgtcaaatt tccacaagca gcaagacctg
     3961 cagccaaagc gactacacta gcagatgcaa aagcatattt tttccagttt ttcatgataa
     4021 aaactccttt ttttattttt aaacttataa acaatgtaat gatcttatac tcaatgaaaa
     4081 tcaaagagca aactagaaaa ctagccgcag gctgctcaaa gcactgcttt gaggttgtag
     4141 ataagactga cgaagtcagt tacatatatc tacggcaagg cgacgttgac gcggtttgaa
     4201 tttgattttc gaagagtatt aacttcacac aagggaagtt gggaactgag aaatgttctt
     4261 tttcaataag cactattctt tcacaccacc gatagtcaaa ccttttacaa agtagcgttg
     4321 gaagaatgga tataaaatcg cgattggaag ggttgcaacc acaaccatgg ccatacgacc
     4381 tgtttctttc ggtagagcaa ctcccagttg accagtcaag ccgaccgctt tggcaatgta
     4441 gtccatattt tgttggattt gcatgagcaa atattgcaat ggatacaagt tatcactctt
     4501 gatgtaaaga agggcgttga accagtcatt ccagaaacca agagctgtca aaagcgtgat
     4561 ggttgcgata cctggtagtg acaatggcaa acagatttgg aagaaaatcc gggcctcact
     4621 ggcaccatcg atacgagccg attcgagaat ggcttctgga atggtcttct tgaagaagga
     4681 acgcatcaag atgatattga atggtgagag aagcattgga acaatcaagg cccaaacagt
     4741 gtcaccaagc tgaagtacac gggtcaccat gatataacct ggtaccaaac cagcgttgaa
     4801 caacatactg agaaggacga agatggtaaa aaatctgcga tacttaaagg ttgtccgtga
     4861 aatagcgtag gcataggttg ttgtgataaa gacatttgtc aatgtcccaa ctacggttac
     4921 aaagacagag atgaagaggg cttgtaggat tttatcctta aactgtgcca aaaactcaaa
     4981 accgtctaag ccaaattggg atgggaagaa gctatagccg tattggagga ggcttttctc
     5041 gtctgtcact gaaataatga taacgaatac aaaaggtagg atacaagaga gggcaatcaa
     5101 acccgaaatg atactgaaga agatatctgc tttcttactg aaggagtgaa tgccgacatt
     5161 atcaattttt tcttttttaa ttttcttttt tgccatattc tcctcctttc tagaacaaag
     5221 ctgagtttgg atcgactcgt cttgcaagca agtttgatag gataaccaga atcaaaccaa
     5281 caacggattg gtaaagaccg gctgctgcag ccataccgat atctgctgtc tgagtcaaac
     5341 cattaaaaac atatacgtcc aaaacgttgg ttacattgta aagctgacca gcattgtgtg
     5401 ggatttgata gaagagaccg aagtctgcgc ggaagatatt tccgactgca aggatggtca
     5461 atacagttac aagcggagtc aactgaggaa tggttacgtt gcgaatacgt tgccacttgc
     5521 tagctccgtc cactgtcgct gcttcgtagt aggttggatc aattcccatg atcgtcgcat
     5581 agtacatgac actgctatat ccaaaacctt tccaaatacc taggaaaagt aggagatagg
     5641 gccagatgcc caggtcagcg tagaaattga cttctttgag accaagactt tccaatagat
     5701 gattgaacac ccctttatca atatttagga aggcatctgt aaagaaactg atgataaccc
     5761 aagacaagaa gtaagggaac aacatagaag tttggaaaat cttcaccatt ctcttagaac
     5821 ggagctcgct gaggataatg gcaatcccta cagatacaac taaacctaga aagataaagc
     5881 caagattgta gaggacagta tttcgtgtga taataaaggc gtctcttgaa ctaaataaga
     5941 atctgaaatt atcgagtccg acccatttac tatttatgat actatctatg aaaccattac
     6001 tggtcatgtg gtagtctttg aaggcaacca cgttcccaaa tactggaatg taaaagaata
     6061 gaatcaacca gagtgcccct ggcaaaacca tcaagagaaa gatccagttg tctctcaatg
     6121 tttttgaaaa ctttttcata atttcctccc tttttatttt gatatccatc taaaaattct
     6181 tttttagact tttgataacg attacattat tagtatactc ctatttgcag gttaggttaa
     6241 actcctaatt atagaaaaaa ctccacaaat tatgtagcag atttaaaact ttatcaccac
     6301 tatcaaacaa atgtcctaaa tcaattgttt attttatctc tattagccca gtgatggcgt
     6361 cactctgtta taagcatcca acaacggggt atactgaaaa atctccagac tagggaactc
     6421 agagatagtt cctaatctgg agatttttaa tatgttatta ggcgtttgct ttcaacttag
     6481 caataacctc tttaagatta tcaatcacct ctgcttcagt atgctcagag cctttttcat
     6541 ctgccaagaa caaaactgct ttttgaagtt ctttttgaga gttttcaagg acatccttat
     6601 ctactgtttc aaggtttgag tctttaagaa gtttacttaa ttccttggct aatttcttga
     6661 gtttgatttg cagactcatc ttctcctgct gtttctttgc ccgctgtttg tcctccatcc
     6721 ttagttgctg actggctttc cttaatggac tctagggaag caatggcatc tttgactgtt
     6781 tgcaagatat cacgtaaacc ttgctctgtc aaactatcat ctgcaaaagc tttcttagcc
     6841 tctgccaaaa ccagacgtgc tgaatctgtg gtaggattcg atacacctgt caatgatctc
     6901 aaaagatttt ctaaggtttg agtctgctta ctaatactag actaaaatca aaaagcatta
     6961 tataacagtg atatgaaatc aactaaagaa gaaatccaaa ccatcaaaac acttttaaaa
     7021 gactctcgta cagctaaata tcataaacgc cttcaaatcg ttctatagta aaatgaaaca
     7081 agaacagggt aaa
//
LOCUS       DAVHIU010000089         7009 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVHIU010000089 DAVHIU010000000
VERSION     DAVHIU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.091"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..596
                     /locus_tag="VVN06_001596"
                     /pseudo
     CDS             1..596
                     /locus_tag="VVN06_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            657..1619
                     /locus_tag="VVN06_001597"
     CDS             657..1619
                     /locus_tag="VVN06_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5563097.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETISKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIA
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1799..2806
                     /gene="gap"
                     /locus_tag="VVN06_001598"
     CDS             1799..2806
                     /gene="gap"
                     /locus_tag="VVN06_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5563098.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2875..3711)
                     /locus_tag="VVN06_001599"
     CDS             complement(2875..3711)
                     /locus_tag="VVN06_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5563099.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNFPILGDPLYNSNSKTSRLMLHAFRLSFTHPLTLEKLNFTALSNTFETELKK
                     NG"
     gene            3837..6032
                     /locus_tag="VVN06_001600"
     CDS             3837..6032
                     /locus_tag="VVN06_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU5563100.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6085..6237
                     /gene="rpmG"
                     /locus_tag="VVN06_001601"
     CDS             6085..6237
                     /gene="rpmG"
                     /locus_tag="VVN06_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5563101.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6247..6423
                     /gene="secE"
                     /locus_tag="VVN06_001602"
     CDS             6247..6423
                     /gene="secE"
                     /locus_tag="VVN06_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5563102.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6478..>7009
                     /gene="nusG"
                     /locus_tag="VVN06_001603"
     CDS             6478..>7009
                     /gene="nusG"
                     /locus_tag="VVN06_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5563103.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2192 a         1239 c         1502 g         2076 t
ORIGIN      
        1 tggctgtcat ccaactacca tccactatgc gctcaaagct atgggctaca ctcgaaaaaa
       61 agaaccacac ctactatgaa caagacccag aaaaagtagc cttatttctt aaaaatttta
      121 atagtttaaa gcacctagca cctgtttaga ttgatgaaac aggattcgat acttattttt
      181 atcgagaata tggtcgctca ttaaaaggtc agttaagaag aggtaaagta tctggaagaa
      241 gatatcagag gatttctttg gttgcaggtc taacaaatgg tgagttaatc gctccaatga
      301 cttacgaaga gatgatgacg agcgactttt ttgaagcttg gtttcagaag tttctcttac
      361 caacattaac cacaccatcg gttattatta tggataatgc aagattccat agaatgggta
      421 agttagaact tttatgcgag gagtttgggc ataaactttt acctcttcct ccctactcac
      481 ctgagtacaa tcctattgag aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat
      541 taccaagttg caataccttt tacgaggctt ttttatcctg ctcttgtttc aattgactat
      601 agttcacgga tacagttggg aaagaagcta ttaaatattt ggagagtcct tttgggatga
      661 acatgtcaaa tattactata gatgggcaag aaatggatct gagtcaaacg atgccttctt
      721 atgctcaagt agtgagtctt tatgatgata caagtatctc tgttagtaat aatgagacgg
      781 acaaagtgtt ggctggctcc ctctatactg atacaaatga acagggatta acgattccaa
      841 gcagtttact aaaaaattgg aatgaacaga caggaaaaaa tttgacagct aatgatctta
      901 ttggcaaatc agtctcagcc agcattgtag aaagtgctgc cgaaactagt aagattgctc
      961 aatttcaaac gaagattgta cgtgtaatca atgatgaaga tgacatggag gacagcaaca
     1021 gtttcatgct gtctcatcaa atggaaacga tttcgaaaga ggctggattt acgaaagctg
     1081 tatcttattt tatcttggaa ctcaaagatc catcacagac aaaagtagta acagaagaat
     1141 tacagaaaaa taagaagtat actgtgcttt ctcaacagag agttcttgat attgtgatta
     1201 cctttattcg tgttattcag ggattattga ttgtgctttc atcacaagct attgtggtag
     1261 cagcggttat gattggtatc attatttaca tcaatatcat gcaacgttcc aaggaaatag
     1321 gtgtcatgaa ggcagttggc tatcagaatc gtgatgtcaa aggaattttt atttacgagg
     1381 ctatctggat tgtaggaatc gccttgctac tggctttttt ggtagcacaa ggggtgggaa
     1441 gtttagcgaa tgcgattgta agtcactttt acccatccat cgctaaggtc tttgaattaa
     1501 atttgttatc tgttttagga actctagttt tcgctctctt gcttggctat gtatcagcct
     1561 acttcccagc acgtaaaatt agtaaaatgg accctgtaga atcgctacgc tatgaatagt
     1621 caagggtgtt actagattat cgtccctaaa aaagaaacta acagagaaaa acaatgcccg
     1681 tacttgcaat tcaactcaaa tagttatata ataggtttgt tgaaaggtga tttgtagtga
     1741 ttcaagttac tcttttcaca ataaaaattt catgattttc ataaggagga aatcactaat
     1801 ggtagttaaa gttggtatta acggtttcgg acgtatcggt cgtcttgctt tccgtcgtat
     1861 ccaaaacgta gaaggtgttg aagttacacg catcaacgac cttacagatc cagttatgct
     1921 tgcacacttg ttgaaatacg acacaactca aggtcgtttc gacggtactg ttgaagttaa
     1981 agaaggtgga ttcgaagtta acggtaaatt cgttaaagtt tctgctgaac gtgatccaga
     2041 gcaaatcgac tgggctactg acggtgtaga aatcgttctt gaagctactg gtttctttgc
     2101 taagaaagaa gcagctgaaa aacaccttaa aggtggagct aaaaaagttg ttatcactgc
     2161 tcctggtgga aacgacgtta aaacagttgt attcaacact aaccacgacg ttcttgacgg
     2221 tactgaaaca gttatctcag gtgcttcatg tactacaaac tgcttggctc caatggctaa
     2281 agctcttcaa gataactttg gtgttgttga aggattgatg actactatcc acgcttacac
     2341 tggtgaccaa atgatccttg acggaccaca ccgtggtggt gaccttcgcc gtgctcgcgc
     2401 tggtgctgca aacatcgttc ctaactcaac tggtgctgct aaagctatcg gtcttgtaat
     2461 cccagaattg aacggtaaac ttgacggatc tgcacaacgc gttccaactc caactggatc
     2521 agttactgaa ttggtagcag ttcttgaaaa gaacgttact gttgatgaag tgaacgcagc
     2581 tatgaaagca gcttcaaacg aatcatacgg ttacacagaa gatccaatcg tatcttcaga
     2641 tatcgtaggt atgtcttacg gttcattgtt tgacgcaact caaactaaag ttcttgacgt
     2701 tgacggtaaa caattggtta aagttgtatc atggtacgac aacgaaatgt catacactgc
     2761 acaacttgtt cgtactcttg aatacttcgc aaaaattgct aaataattct tgaatcgata
     2821 aaaagcaagg cctcttggtc ttgcttttct tatatggaaa aatggatgac acaatcatcc
     2881 attctttttt aattctgttt caaaagtatt tgagagggca gtgaagttta atttttctaa
     2941 tgtaagtgga tgggtaaagg aaagtcgaaa ggcatgaagc ataagccgac ttgtctttga
     3001 attgctgtta tagagagggt cgcccaaaat agggaagtta tgatgcgaaa ggtgcacacg
     3061 aatctgatgg gttcgccctg tctttagctt gcaatgagcc aaggaagtct tgtttgagaa
     3121 ttgctttaat ctgcttacat acgtttcagc atattgccca ttttttgcat caactattct
     3181 ttttctacga tcatggcgat cacgtccaat tttgtctctg aaaacaagtt cttttctgtt
     3241 gatatttcca tcaactagag cccaatattc tctagaaatc tcttttttct ccaataagcg
     3301 attgagaatg ggcaggataa aaggattttt ggcaaagaga actaagccac tggtttccat
     3361 gtccagacga tgaacgacat agcaggtttg gccaacatag gtactgacat ggttaagaag
     3421 ggcaatttcg tttggttgat taccatgcgt tttcatcccc tctggtttgt ttacaataat
     3481 caagtgttga tcttgataaa cttcctgcac taagtctggg ttgccccaag ggatcgtctt
     3541 ttgggaataa tcttcctcgt caaaagtcaa ctggcaaaca tctccaggat ttacgatttc
     3601 gttccagtgg acttcttctt gatttatcaa aatatgtttc ttgattctca aaaaatgacg
     3661 gatttttcta gggatgagga gttgttcctc aagtaattgc tttaccgtca tttgaggtag
     3721 agaggcgggt aatgtaaatg tgaattgcat acagatattg taacaaaaaa agccctattt
     3781 ggataggaaa tagctaaatt cttgtcttcc tatgatgaag atgataaaat aaacgcatga
     3841 aattagataa attatttgag aaatttcttt ctctttttaa aaaagaaaca agtgaactag
     3901 aggactctga ttctactatc ttacgtcgct ctcgtagtga tcgaaaaaaa ttagcccaag
     3961 taggtccgat tcgaaaattc tggcgtcgtt atcatctaac aaagattatc cttatactag
     4021 gtttgagtgc aggattgcta gttggaatct atttgtttgc tgtagccaag tcgaccaatg
     4081 tcaatgattt gcaaaatgcc ttgaaaactc ggactcttat ttttgaccgt gaagaaaaag
     4141 aggctggtgc cttgtctggt caaaagggaa cctatgttga gctgactgac atcagtaaaa
     4201 acttgcagaa tgctgttatt gcgacagaag accgttcttt ctataaaaat gacgggatta
     4261 actatggccg tttcttcttg gctattgtca ctgctggacg ttcaggtggt ggctctacca
     4321 ttacccaaca gctggctaaa aacgcctatt tatcgcagga tcaaactgtt gagagaaaag
     4381 cgaaagaatt tttccttgcc ttagaattaa gcaaaaaata tagtaaggag caaattctaa
     4441 ccatgtacct taacaacgct tattttggaa atggtgtgtg gggtgtagaa gatgcgagta
     4501 agaaatactt tggagtttct gcatcagaag tgagtctgga tcaagctgcg actctggcag
     4561 ggatgctcaa ggggccggaa ctgtataatc ccttgaattc tgtagaagat tctactaatc
     4621 ggcgcgatac tgtcttgcag aatatggttg cagcaggata tattgataaa aaccaagaaa
     4681 ccgaagctgc tgaagttgat atgacttcgc aattgcacga taagtatgaa ggaaaaatct
     4741 cagattaccg ttacccctct tattttgatg cggtggttaa tgaagctgtt tccaagtata
     4801 atctaacaga ggaagagatt gtcaataatg gctaccgcat ttacacagag ctggaccaaa
     4861 actaccaagc aaatatgcag attgtttatg aaaacatatc gctatttccg agggcagagg
     4921 atggaacgtt tgctcaatca ggaagtgtag ctctcgaacc gaaaacaggg ggagttcgtg
     4981 gagttgtcgg tcaagttgct gacaatgata aaactggatt ccggaatttc aactatgcaa
     5041 cccaatcaaa gcgtagtcct ggttctacaa ttaagccttt agttgtttat acgccagcag
     5101 ttgaagcagg ctgggctttg aataagcagt tggataacca taccatgcag tatgacagct
     5161 ataaggttga taactatgca gggatcaaaa cgagtcgaga agttcctatg tatcaagcct
     5221 tggcagaatc gcttaatcta cctgctgttg ccactgttaa tgatttgggt gtcgacaagg
     5281 cttttgaggc aggcgaaaaa ttcggactca acatggaaaa ggttgaccgt gttcttggtg
     5341 tcgccttggg aagcggtgtt gaaaccaacc ctcttcaaat ggctcaagca tacgccgcct
     5401 ttgcaaatga aggtttaatg cctgaagctc attttattag tagaattgaa aatgctagtg
     5461 gacaagttat tgcgagtcat aaaaattcac aaaaacgggt gattgataag tctgtagctg
     5521 acaagatgac cagtatgatg ttggggactt tcaccaacgg taccggtatt agttcatcgc
     5581 ctgcagacta tattatggca gggaaaactg gaacaactga agcagttttt aatccggagt
     5641 acacaagtga ccagtgggta attggttata ctccggatgt agtgattagc cactggcttg
     5701 gctttccgac cactgatgaa aaccactatc tagctggctc tacttcaaac ggtgcagctc
     5761 atgtctttag aaacattgcc aataccattt taccttatac gtcaggaagt acctttacgg
     5821 ttgaaaatgc ttataagcaa aatggaattg caccagccaa tacaaaaaga caagtacaaa
     5881 ccaatgataa tagccagaca gatgataatt tgtctgatat tcgagggcgt gcgcaaagtc
     5941 tagtagatga ggctagccgg gctatctcag atgcgaagat taaggaaaag gctcaaacaa
     6001 tatgggattc gatagtcaat ctatttcgct aagatgcttg tcaaagccta gctttcttgt
     6061 tataatggat aagatggagg cgttatggca ctaaaaaaag caagcctagc ttgtgcggtt
     6121 tgtggttcga gaaactattc aatcaagatc agcggaaacc ccaagcctac acgactagaa
     6181 gtaaataaat tttgtaagca ttgtggcaag tacactacac atagagaaac gagataggag
     6241 agagcgatgc gttttattgg agatattttt agacttctta aagacacaac gtggccaact
     6301 cgcaaggaaa gctggagaga ttttcgttct atcatggaat acacagcttt ctttgtagta
     6361 attatttaca tttttgacca gttgattgtt tcaggtttga ttcgatttat taacattttt
     6421 tagaagattg gtggagttaa ttacactaga aatcttctat ttatgaaagg aaatatcatg
     6481 gatagttttg ataaaggatg gtttgtttta caaacttatt ctggttatga aaataaggtg
     6541 aaagaaaatc tattacaacg tgcacaaacc tacaatatgt tggataatat tctacgcgtt
     6601 gaaattccaa cacaaacagt gcaagttgaa aaaaatggaa agagaaaaga agtagaagaa
     6661 aatcgctttc caggttatgt tcttgtagaa atggtcatga cagatgaagc ttggtttgtt
     6721 gttcgaaaca caccaaatgt tacaggattt gtcggatcac acgggaacag atcaaaacca
     6781 actccattat tggaacaaga aattcgtgac attttggtat ctatgggaca aactgttcaa
     6841 gaatttgatt tcgatgttga gattggtcaa actgtacgta ttattgatgg tgcttttgca
     6901 gactacactg gtaagattac agaaattgat aataacaaag tgaaaatgat tatctctatg
     6961 tttggtaatg acacagttgc agaagtaaac ctaaaccaaa tcgcagaat
//
LOCUS       DAVHIU010000090         6931 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVHIU010000090 DAVHIU010000000
VERSION     DAVHIU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.092"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(138..1019)
                     /locus_tag="VVN06_001604"
     CDS             complement(138..1019)
                     /locus_tag="VVN06_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU5563104.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(1160..2488)
                     /gene="vncS"
                     /locus_tag="VVN06_001605"
     CDS             complement(1160..2488)
                     /gene="vncS"
                     /locus_tag="VVN06_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU5563105.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVVQD"
     gene            complement(2485..3141)
                     /gene="vncR"
                     /locus_tag="VVN06_001606"
     CDS             complement(2485..3141)
                     /gene="vncR"
                     /locus_tag="VVN06_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU5563106.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCIFTVRNVGYKLERK
                     "
     gene            complement(3190..3273)
                     /gene="pep27"
                     /locus_tag="VVN06_001607"
     CDS             complement(3190..3273)
                     /gene="pep27"
                     /locus_tag="VVN06_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU5563107.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(3452..4831)
                     /gene="vex3"
                     /locus_tag="VVN06_001608"
     CDS             complement(3452..4831)
                     /gene="vex3"
                     /locus_tag="VVN06_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU5563108.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(4883..5530)
                     /gene="vex2"
                     /locus_tag="VVN06_001609"
     CDS             complement(4883..5530)
                     /gene="vex2"
                     /locus_tag="VVN06_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU5563109.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(5543..6820)
                     /locus_tag="VVN06_001610"
     CDS             complement(5543..6820)
                     /locus_tag="VVN06_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563110.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYDSSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         1930 a         1528 c         1351 g         2122 t
ORIGIN      
        1 ttatcctatt ctagtttcaa aacgctatag aagcagcatt gtgctagtct agattcagtt
       61 tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc aggttttatc
      121 tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat acgttcttca
      181 accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc gaagagtttt
      241 ttcttgtcgt attctgcttc gtttgcttcg taatcacgag caaatttacg agttgcgtta
      301 gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag tttgatagct
      361 gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg gaatcctgga
      421 agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt tactgggtaa
      481 gggccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc aaccattgct
      541 ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc ttcaccaccg
      601 atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc aacaacttct
      661 ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat gattgaagta
      721 taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag gtggatagct
      781 actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg agcaactttg
      841 taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg agctttttta
      901 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacca
      961 actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac gattgccatt
     1021 ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca ttttatcact
     1081 ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct aactccatct
     1141 atgtaaaccc tttctctccc tagtcttgga cgacttttgg aaaagctata aagaaggtta
     1201 aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat agactcttaa
     1261 ccacaaaaag ccccatacca gaccccttga ccttgcgact ggcattgtca gaaaaagact
     1321 gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt tctccttctc
     1381 tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg ctgatgagat
     1441 tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg gtcaaactat
     1501 tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc agatttttcg
     1561 tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca gaagagaggg
     1621 aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca actcccagat
     1681 actggtctct atccttataa cgaccgatat tctctctcat attttcgatt aggattttca
     1741 aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg accttcatct
     1801 tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca gtcaagagat
     1861 gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta gaatccacgc
     1921 gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg atttccaaaa
     1981 tcggtgcaac aatagtccga gcgtagatgt aggccaccaa gagggaaatc agaaaggagg
     2041 ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt tgtaaatcca
     2101 tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc tcgcgctcct
     2161 caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc ttgacttcta
     2221 acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc tgggaataca
     2281 agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg gcaatggctg
     2341 ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga aaaagaaaat
     2401 aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta tagataaata
     2461 tctttgtaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa cattgcgcac
     2521 agtgaagata caatccaagt ctagcttttt ccgcaattcc ttgatataaa catcaataac
     2581 acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct gagaccgagt
     2641 caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt tggcattgat
     2701 aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct tggtatcctt
     2761 gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt
     2821 taagagggag agggagaaag gtttttccag atagccatct gccaaagagg caaaggcact
     2881 catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct gactggtttt
     2941 acgaatctca gctaggactt ctaagccgtt aagcttgggc atctggatat ccagtaaaac
     3001 cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat
     3061 agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca tctcttcatc
     3121 ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt cttagaataa
     3181 atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa gcaactgacc
     3241 actagataaa acgttgtgaa attcctttct cataaattcc ataactttag tatattatat
     3301 ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac tgctttcgat
     3361 ttattttgaa ttgttaaata gctattccta tccactattc ttgaatagaa acacaagatg
     3421 caatctttat tccagactca ttttttcaaa tttattcacc atccagcaag agctcttttg
     3481 gttgttttct aaggagattg cttgaagcaa gcgccataac gagaaccact agaaccaagg
     3541 caaggacaaa aatgatgata aagtctgatg tctgaatgga aatgtctagg ctcgacaagg
     3601 tcttgctaaa gccatctact tctgcaccgc caccaaggtt agaggcttga gccgccttac
     3661 tagcctgttt ggcaacacct gaagtcacat tggcaaggac agtgtttcca attgcacggg
     3721 cagtgtaatt agctagaaag taagcagaaa ctagagcagg gatagcaatc aagatagatt
     3781 cggttatgaa ttgacccaag atacttgcct gcttgaggcc gatagagagg agaattccca
     3841 cttccttgcg acgggcgttg atccaaaggc tgagcaagag ggcaaggagg agaactgaga
     3901 agctcaagct accccagaag aggaggttgg ccatcttgta cataccagag atagattgct
     3961 caagagctgg gtagttagag gagctcttga cgagtgtgta gctcttccag ttgataccac
     4021 tgatgccatt caactctttc ataacatcat ccaagttctt gtctgctgtt acaaagaagg
     4081 ttgcgtcccc ataaatggct gtgtcttctg tgtatccata aagttttgca gcagtgtgaa
     4141 tgtctgtaat agctgtgttt tcgtaaagtt cttgtgagta ggttactgct gacttattat
     4201 gaccatcaaa gagtcccttg attgtcactt caactgtttc cttggctcct ttttcattat
     4261 ctgcatcgta gatattagag tccagtttaa ccttgtcccc tactttccag ccgtgtttgg
     4321 ctgccaagtc cttgtgcaag aggattttat ccttgtcgtc gttggttaag tgctctcctt
     4381 cgactagttt ataagaacca gagacaaact tgtcttcttt agaggagtca ttgacacctg
     4441 taatcatcaa gctacttcca aaacgtttgg cacgatcagc agtgagattc ttcttggttt
     4501 ctggcgtttc aatcaggtca tatccagtca aatctccgat agcgttgata cgtttgacat
     4561 aagactcaat ggccttgttt tcggtgattt ttttgatgtc ttcacccttg atattcccag
     4621 caccacgagg cgttccttgg ttgacgcgac gattgatttg catggagaag ctattggtga
     4681 tatttttaaa ggtctcctga gaagccttgg cagtagctcc cttgattgac aagccgacca
     4741 aactcaagct cgccataagg agaataatca ggaagatgac aatcgatttg aaaaacttcc
     4801 ttgtaacata ggcaaatgcg ttgtgtaaca tagattccct ttctagattt tgttttaatc
     4861 attctattaa aataagctca aattatttac tagtattgcg cgtttcagtc agtttcttat
     4921 cctttaattc aagtgtaata tctgacgctt gtgccacttc tttactgtga gttacgacaa
     4981 tcacacattt acctgttttc tgggcaagtg atttgagtag ttcgacaata tctccagcag
     5041 ttttaggatc cagatttcct gttggctcat cagctagaat aactggagct tctgagacca
     5101 aactgcgagc aatggcaaca cgttgctgtt gaccacctga taactggaga acattccgct
     5161 tgatctggct ttcatccaaa ccaagctcaa gaagtgtatc cttgcttgcc tttttgttga
     5221 ccaagcggat attttccagc ggagaaagat aatctatcaa gttataattt tgaaagacca
     5281 gggaaatatg gtgcatgcga tggtaagaat agcccttctt acgaatatcc tctccttgaa
     5341 aaaggataga accttcaaca ggactatcta gaccagcaag tagggacaag agtgtggatt
     5401 ttcctgctcc tgactcccca ataatactgt aaaattttcc gggttcaaaa ttataatcga
     5461 tctgatatag gactgcttca gcagtattct tataacggta ggtaacatct tgtaattgta
     5521 ataaagtcat gatttctcct tcttaactaa tagatgataa aatttctttc ggtgattttc
     5581 taaataagaa taggaaacaa agggctacag ataagcaact aagcagaact agaaaaacat
     5641 aggattctgc aaaagataag atgctagttg ataaactgct tgctttggct agtgtatctt
     5701 gtaagcttgc ctgatctcca cttgctagta gagtttggag taggtaagtt gtgattccgt
     5761 ttcctgcaac aaatgctgga agcaaagctc caagagatac caaaactacc tctaaacaga
     5821 attgtaggaa gatcgagctc ttgccttttc caagtgcaag taaaatcccc acttcataga
     5881 cccgttctct caaccagaga gacaaaacca gaattaaggc tccagctcct gctatcaaca
     5941 tcccataaag gaagatggtc aggaaggttt ggaaagttgc aactgagtct ttgatttgtt
     6001 caaaagcctt gttttccttt tcgacttggt agccttgatt ttccaaggcc aagttttcta
     6061 cctgcttcat gagtccgtcc atttccttag gattttctac atagaagcgt gctgcactga
     6121 cttgagcttc actattgccc aaaagggttt ggctactgtc atagtctgta aagacttgat
     6181 tttcactgaa gtcagaagac aagcctgtga atttctcttg ttttttacca gaaaagatgc
     6241 cgataatctc aaactctact gtttgtcctt ttccagattc agactgacca gcatccaagc
     6301 caatcttgtc atgaagcgaa agaccgttct tcttagccaa ttcttcgtgg ataaggattt
     6361 tcttggaatc ccctttttga aggtgtcgcc cttcttttag attgaaagcc gaactggtaa
     6421 aggttacatc cttggatgaa tcctcaagag ccgttaagct aaccaagtta ttgtctgcag
     6481 ctgataaatc atcacgctcc acgctctgct cgccagtcac tgcttccttg tcttttagtt
     6541 ttgcgaccgt ctcaagttca ggagagacat tttccagccc cttaatcttg cttacagatg
     6601 ctaggtctga caacttgaat gtctgaccat tctctatctt cttaatagaa aaagatgtat
     6661 tgagtgattt ataaagattg ctttctactg ttttgttgga cttcatcaga gtcaaacagg
     6721 ctgaaattcc ggccaataag accaataaaa tcagaaataa aataaaactt ctcagtcgct
     6781 ttctgctgac ataagcccaa gatctttgga ttggattcat ttgtcacctc catatttgta
     6841 agactattat aaaacccaaa tatgaaatgt ttatgaaata cgaaaaaaaa atatcgagta
     6901 ggggataatc tctagcccct ctcacaccac c
//
LOCUS       DAVHIU010000091         6794 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVHIU010000091 DAVHIU010000000
VERSION     DAVHIU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.093"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..292
                     /locus_tag="VVN06_001611"
                     /pseudo
     CDS             1..292
                     /locus_tag="VVN06_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            459..659
                     /locus_tag="VVN06_001612"
     CDS             459..659
                     /locus_tag="VVN06_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563111.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            684..1100
                     /locus_tag="VVN06_001613"
     CDS             684..1100
                     /locus_tag="VVN06_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563112.1"
                     /translation="MKAALESLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1331..2038
                     /gene="ccdA2"
                     /locus_tag="VVN06_001614"
     CDS             1331..2038
                     /gene="ccdA2"
                     /locus_tag="VVN06_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU5563113.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2049..2606
                     /gene="sdbB"
                     /locus_tag="VVN06_001615"
     CDS             2049..2606
                     /gene="sdbB"
                     /locus_tag="VVN06_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU5563114.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2980..4371
                     /locus_tag="VVN06_001616"
     CDS             2980..4371
                     /locus_tag="VVN06_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5563115.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4514..5431
                     /gene="adcAII"
                     /locus_tag="VVN06_001617"
     CDS             4514..5431
                     /gene="adcAII"
                     /locus_tag="VVN06_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU5563116.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5439..>6794
                     /gene="phtD"
                     /locus_tag="VVN06_001618"
     CDS             5439..>6794
                     /gene="phtD"
                     /locus_tag="VVN06_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU5563117.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NK"
BASE COUNT         2091 a         1248 c         1431 g         2024 t
ORIGIN      
        1 ccagaaaaag tagccttatt tcttaagaat tttaatagtt taaagcacct agcacctgtt
       61 tagattgacg aaacaggatt tgatacttat ttttatcgag aatatggtcg ctctttaaaa
      121 ggtcagttaa gaagaagata tcagaggatt tctttggttg caggtctaac aaatgaatgg
      181 tgagttaatc gctccaatga cttacgaaga gacgatgacg agcgactttt ttgaagcatg
      241 gtttcagaag tttctcttac caacattaac cacaccatcg gttattatag taaaatgaaa
      301 taagaacagg acaaatcgat caggacagta aaatcgaatt ctaacaatgt tttagaagtc
      361 tatatgtact attctagttt caatatacca taactgctcc tagtcaacct gcaagtcctg
      421 tcccagctcc aagctcagct ccgactcaag atcctagagt ggtagcaagc gcttcggcta
      481 gctcaacgag cacacaagct caagagcaag ttgacaagtc tgaacttcgt gccttgagtc
      541 aagagttaga ccaacgcttg aaagctttgg caacagtatc tgatccaaaa atcgatgcaa
      601 ccaaggctgt cctcctagat gctcaaaaag ctccggaaga tagtgccttg acagagtaag
      661 gtctgcgtac tgcagtagag tctgtgaagg ctgccttgga gtctctcaaa gatgtgaaag
      721 caaatgctag cgatagcaag cctgcacagg acaagaagga tgcaaaacaa ggaacggaag
      781 atagtaagga ttcagataag atgactgaaa caaactcagt tccagcagga gtgattgtgg
      841 tcagtctact tgccctccta ggcgtgattg ccttctggct gattcgccgt aagaaagagt
      901 cagaaatcca gcaattaagc acggaattga tcaaggttct aggacagcta gatgcagaaa
      961 aagcggataa aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc cttgatttcg
     1021 tgaaagaaga aaatggctca gcagagacag aaactaaact agtagaggag cttaaagcaa
     1081 tccttgacaa actcaagtaa aacatgattt atgaaatgtc agacctaaaa gacattttcg
     1141 ttcacaaaag cagcgatgag taatccttgt cgttgctttt tggctatttt gggggataag
     1201 atccgccttg actagcaaaa gaaaagattg gtccaatagt gcccgatacg attttgcgta
     1261 gcagaaagct tacaaataaa attgaatata gtaaaatagg attagaatta ttaagaaagt
     1321 tggttcttta ttggaaacga tagtattttt aatctctgtt tttctagcag gtgttttatc
     1381 ctttttttct ccttgtattt ttcctcttct gccagtctat gctgggattt tattggatga
     1441 tcaagaaagt gcaaaaagct tttctttgtt tgggagaaag gttctctggt caggcttgat
     1501 tcgaacactt tgctttatcg ctggtatctc tctcattttc tttattctag gctttggtgc
     1561 tggttacttt ggtcatattc tctatgcaaa ttggtttcga tatggcatgg gagctattat
     1621 tatcattttg ggtcttcacc agatggaaat ttttcatttg aagaaattag aagttcaaaa
     1681 aagttttacc tttaaaaaat cagactctaa tcgttattgg tcagcttttt tacttggtat
     1741 tacctttagc tttggttgga caccttgtat tggtccagtt ttaagttctg ttttagcact
     1801 tgcggcttct ggaggcaatg gcgcttggca aggagcgatt tatactctca tttacactct
     1861 gggcatggcc cttcctttct tggtattggc actagcttca ggtctagtca tgccatattt
     1921 tagtaaaatc aagcgtcata tgatgctact aaagaaaatt ggtggtttcc tcattgtttt
     1981 aatgggaatt ttgttactat taggacaagt aaatgttcta gctggaattt ttgaataaag
     2041 gaggacaaat gaaaaaagta atgtttgctg gcttaagtct cttgtcatta gttgtattga
     2101 tggcctgtgg tgaggaagaa actaaaaaga ctcaagcagc acaacagcca aaacaacaaa
     2161 cgactgtaca acaaattgct gttggaaaag atgctccaaa cttcacattg caatccatgg
     2221 atggcaaaga agttaagtta tctgatttta agggtaaaaa ggtttacttg aagttttggg
     2281 cttcatggtg tggtccatgc aagaaaagta tgccagagtt gatggaacta gcggcgaaac
     2341 cagatcgtga tttcgaaatt cttactgtca ttgcaccagg aattcaaggt gaaaaaattg
     2401 ttgagcaatt cccacaatgg ttccaggaac aaggatataa ggatatccca gttctttatg
     2461 ataccaaagc aaccaccttc caagcttatc aaattcgaag cattcctaca gaatatttaa
     2521 ttgatagcca aggaaagatt ggaaagattc aatttggtgc tatcagtaat gcggatgcag
     2581 aagcagcatt taaagaaatg aactagaatc gataaaatct gattacaaga tgtagtcaga
     2641 ttttggtaac ttctaaaact aacttccagt ttcccacaac ctagctttta gtatgagaaa
     2701 aacgcgtgac atcagtggaa atccgtctta gactaacttc cactggtttt ctttttcttg
     2761 atatataagg gttcaaaagc gaaaagactc agaaaaagtg cacgacaaat agccctaaaa
     2821 cagagtcgtc ttagagtaac ttccagtttt gggagagaga tggaagttac tttgagaagt
     2881 tacggtagtc agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt
     2941 atggtaaaat agaatcatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag
     3001 aatgttagtt tagataaaac agagatgcat aggcatttga agttatggga tttgattttg
     3061 ctgggtatcg gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca
     3121 acacttgctg gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta
     3181 tcagccctct tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt
     3241 tacctctatg ctatcttagg agaattccct gcctggttgg ctggttggtt aaccatgatg
     3301 gagttcatga cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg
     3361 ctctctcaat acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca
     3421 gggacatttg ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta
     3481 ctaaatgcca aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct
     3541 ttagctctct ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt
     3601 gctccttacg gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc
     3661 ttgatgttct tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag
     3721 actcctcaaa aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc
     3781 tatgccttgg tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat
     3841 gatgccgttg cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca
     3901 ttagtggcta tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt
     3961 atgatttaca gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag
     4021 actagcaaga taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca
     4081 ggaatgttcc cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg
     4141 atcatgttgg cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg
     4201 gaatttaaaa caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc
     4261 ttcatgttac agtataatat gaatacctgg attgctttcc tagttgcgtt gttagtagga
     4321 agtattattt acttcactta tggctataag cattctacca tagaagaata aagtgaaagt
     4381 ctattgagtt gttgacactc agcagatttt tatatgtgaa agaaatttca aattttttct
     4441 aaaaatatct tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt
     4501 taaggagttg ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt
     4561 gtgcttgggg gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac
     4621 cagtttttat cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg
     4681 gatgattcag tcaagtagtg gtattcactc ctttgaacct tcggcaaatg atatcgcagc
     4741 catctatgat gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag
     4801 tctggatcca aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac
     4861 cttggaacgt gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac
     4921 gctctatgac cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat
     4981 cgctgataaa ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca
     5041 agcctttatc aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc
     5101 gactcagaaa acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg
     5161 gcttaatcaa cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact
     5221 aacagaaatt caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa
     5281 cgcttcttca aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct
     5341 gaatccttta gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa
     5401 tatgagtatt ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc
     5461 tagcaggttc agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc
     5521 aagctggtca ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg
     5581 gtcaaaaggc agaaaatttg acaccagatg aagtcagtaa gagagagggg atcaacgccg
     5641 aacaaattgt tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc
     5701 attactataa tggcaaggtt ccttatgatg ccatcatcag tgaagaactt ctcatgaaag
     5761 atccgaatta tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggctatgtga
     5821 ttaaggtaga cggaaaatac tatgtttacc ttaaagatgc ggcccatgcg gacaatattc
     5881 ggacaaaaga agagattaaa cgtcagaagc aggaacgcag tcataatcat aactcaagag
     5941 cagataatgc tgttgctgca gccagagccc agggacgcta caccacagat gatggttata
     6001 tctttaatgc ttctgatatc attgaggaca cgggtgatgc ttatatcgtt cctcacggcg
     6061 accattacca ttacattcct aagaatgagt tatcagctag cgagttagct gctgcagaag
     6121 cctattggaa tgggaagcag ggatctcgtc cttcttcaag ttctagttat aatgcaaatc
     6181 cagctcaacc aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa
     6241 atcaagggga aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac
     6301 gccatgtgga atctgatggc cttattttcg acccagcgca aatcacaagt cgaaccgcca
     6361 gaggtgtagc tgtccctcat ggtaaccatt accactttat cccttatgaa caaatgtctg
     6421 aattggaaaa acgaattgct cgtattattc cccttcgtta tcgttcaaac cattgggtac
     6481 cagattcaag accagaacaa ccaagtccac aatcgactcc ggaacctagt ccaagtctgc
     6541 aacctgcacc aaatcctcaa ccagctccaa gcaatccaat tgatgagaaa ttggtcaaag
     6601 aagctgttcg aaaagtaggc gatggttatg tctttgagaa gaatggagtt tctcgttata
     6661 tcccagccaa ggatctttca gcagaaacag cagcaggcat tgatagcaaa ctggccaagc
     6721 aggaaagttt atcgcataag ctaggagcta agaaaactga cctcccatct agtgatagag
     6781 aattttacaa taag
//
LOCUS       DAVHIU010000092         6772 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVHIU010000092 DAVHIU010000000
VERSION     DAVHIU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.094"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            32..2011
                     /locus_tag="VVN06_001619"
     CDS             32..2011
                     /locus_tag="VVN06_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5563118.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2078..3574
                     /locus_tag="VVN06_001620"
     CDS             2078..3574
                     /locus_tag="VVN06_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563119.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3574..4500
                     /locus_tag="VVN06_001621"
     CDS             3574..4500
                     /locus_tag="VVN06_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563120.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINVVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4509..5576
                     /locus_tag="VVN06_001622"
     CDS             4509..5576
                     /locus_tag="VVN06_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563121.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5587..6513
                     /locus_tag="VVN06_001623"
     CDS             5587..6513
                     /locus_tag="VVN06_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563122.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(6588..>6772)
                     /locus_tag="VVN06_001624"
     CDS             complement(6588..>6772)
                     /locus_tag="VVN06_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5563123.1"
                     /translation="KIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFI
                     ALNIKKERTKFVLSRA"
BASE COUNT         1998 a         1264 c         1421 g         2089 t
ORIGIN      
        1 tctataattc acataaacag aggagtagaa aatgaaaaaa aatcgtgtat ttgctacagc
       61 aggtcttgtt ttattagcag caggtgtact tgcagcatgc agttcttcaa aatcatctga
      121 ttcatcagcc cctaaagctt atggctatgt ttatacagca gacccagaaa ccttggacta
      181 cctgatttca agtaaaaata gtacaacagt agtgacttca aatgggattg atggtttatt
      241 cactaacgat aattacggta atcttgctcc tgcagttgca gaggattggg aagtctctaa
      301 ggatggtttg acctacactt ataagattcg taaaggggtt aaatggttta cctctgatgg
      361 agaagaatat gcagaggtga cggctaaaga tttcgtgaac ggtttaaaac acgcagcaga
      421 taaaaaatca gaagctatgt atttagctga aaattcggtt aaaggcttgg cagattatct
      481 atcaggaact tcaacagatt tttcaacagt tggtgtcaag gcggttgatg attatacgtt
      541 acaatacact ttgaaccagc ctgaaccgtt ctggaactct aagttgacct attctatttt
      601 ctggcctctg aatgaagaat tcgaaacatc aaaaggaagc gattttgcta aaccaacaga
      661 tccgacatcc ttgctttata atggtccatt cttgttgaaa gggttgactg caaaatcttc
      721 tgtagagttt gtaaaaaatg agcaatattg ggataaagaa aatgtccacc tagatactat
      781 taatctagct tactatgatg gatcagatca ggagtcgcta gagcgtaact tcactagtgg
      841 agcttatagt tatgcccgtc tttaccctac cagctccaac tattctaagg ttgcagaaga
      901 atacaaggac aatatctatt acacacaatc aggctctggg attgctggtc tgggtgtgaa
      961 tattgatcgc caaagttaca actatacttc taaaactaca gattcagaga aagtagctac
     1021 taagaaggca ttgcttaaca aagatttccg tcaagccttg aattttgctc ttgatcgctc
     1081 agcttactca gctcaaatca atggtaaaga tggagcagct ttagcagttc gtaatttatt
     1141 tgtaaaacca gactttgttt cagctggtga gaagaccttt ggtgatttag tcgctgctca
     1201 acttcctgct tatggtgatg agtggaaagg tgtgaattta gctgatgggc aggatggttt
     1261 attcaatgct gacaaggcca aggcagagtt tgcgaaagct aagaaagctt tagaagcaga
     1321 cggcgttcag tttcctattc atctggacgt tccagtagac caagcatcaa aaaactacat
     1381 atctcgtatt cagtccttta aacaatctgt agaaacagtt cttggtgttg aaaatgtcgt
     1441 tgttgatatt caacaaatga caagtgatga attccttaat attacttact atgctgccaa
     1501 tgcttcatct gaggattggg atgtatcagg aggagtttca tgggggccag actatcaaga
     1561 cccatctact tacctggata ttttaaaaac aactagcagt gaaactacaa aaacatattt
     1621 aggatttgat aatccaaata gcccttcagt agttcaagtt ggtttgaaag aatacgataa
     1681 attagttgat gaagctgcca gagagacaag cgacttgaat gtccgttatg aaaaatatgc
     1741 agcggctcaa gcatggttga cagatagttc actctttatt cctgctatgg cttcttctgg
     1801 tgcagcacca gtgctttcac gaattgttcc atttactgga gcttctgcgc aaacaggctc
     1861 taaggggtca gatgtttact tcaaatattt gaaattacaa gataaagtgg tgactaagga
     1921 agagtatgaa aaagctcgtg aaaaatggtt gaaagaaaaa gctgaatcaa atgagaaagc
     1981 tcaaaaagaa ttggcaagtc atgtgaagta aataatttct aatagaactt tctctccatg
     2041 aggagaaggt tcttttggga tttttaaagg aaataatatg aaaaaatata tttttatgcg
     2101 tgttttgcgg tcattggttt cgattttctt agtaacgact ttgacctaca cgattatcta
     2161 taccttggtt cctcgaaaat tgattttcaa gcaggatcct aactataata aaattgcgac
     2221 aacggctgat aaacgtgata actatgaaaa tactgtgttt gagcgtatgg gctacattga
     2281 gtattacgat actaaagagt tgcaagaaaa ggcaagtagc atggattctt ctgtaacagt
     2341 agaagcaaat gcgaccaata aagctattta tgaaaagtac atcaatcaat taggtcatgg
     2401 ttggactttg ggagaattta ctgaaagtgg tcaattctat gctactcgtg aaattccaat
     2461 ttttgaacgt gtttttcact tctatgctaa cttgattgac attgatcata caaataaaat
     2521 ccaagaccct gaaaatccag acttgaaacg ctaccttcgt tttgaaaatg atccagctat
     2581 cggatggtca ttggtcggtt caggaactaa acataaatat ctcttgtact ttaacagtca
     2641 gttcccattt gttcatcaaa actttgtgaa cttgaattta ggtgactctt acccaaccta
     2701 tgctaataca ccagttcttc aggttattac tcaaggtcaa ggacaaacca aaactgccca
     2761 agttcagttc ccaacaggta agaaaacgtc ttctgtaaat atttactcaa gaacctacaa
     2821 gtcacctagt caggctgact ctcgtgaagt agctagctat gggaaagatg atccttatac
     2881 agcgactgaa agtaattacc aatatccatc tatgattgtc agctctgcta ttactggttt
     2941 gattggtttg gttcttgcct atgctcttgc cgtgccactt ggttcagcca tggctcgttt
     3001 caagaacact tggattgata gcctctcaac agggactttg accttcttgc ttgctcttcc
     3061 aacgattgcc ttggtttaca tcgttcgatt gattggatca tctattgccc ttccagattc
     3121 attccctatc ttgggagctg gagattggcg ttcttacgtt ttaccagcag tcatccttgg
     3181 tttgttgggt gctcctgtta cagccatttg gattcgtcgt tacatgattg acttgcaatc
     3241 tcaagacttt gttcgtttcg ctcgtgcaaa aggtttgtct gaaaaagaaa tttcaaacaa
     3301 acacatcttt aaaaatgcca tggttccgct ggtttcagga attcctggtg ccattattgg
     3361 ggttatcggt ggtgcaaccc ttactgaaac agtcttcgcc ttcccaggta tgggtaaaat
     3421 gttgattgac tctgtaaaag catctaataa ctctatggtc gttggtcttg tcttcatctt
     3481 tacatgtatt tctatcttct cacgtctttt gggagatatt tggatgacta ttattgaccc
     3541 acgtattaaa ttgactgaga aaggaggcaa ataatgtcta caatcgataa agaaaaattt
     3601 cagtttgtaa aacgtgacga ttttgcctct gaaactattg atgcgccagc atattcttac
     3661 tggaaatcag tgtttaaaca atttatgaag aaaaaatcaa ctgtagtcat gttgggaatc
     3721 ttggtagcca tcattttgat aagtttcatc tacccaatgt tttctaagtt tgatttcaat
     3781 gatgtcagca aggtaaacga ctttagtgtt cgttatatca agccaaatgc ggagcattgg
     3841 ttcggtactg acagtaacgg taaatcgctc tttgacggtg tctggttcgg agctcgtaac
     3901 tccatcctca tttctgtgat tgcgacagtg attaacgtgg ttatcggtgt ttttgtcggt
     3961 ggtatttggg gtatttcaaa atcagttgac cgtgtcatga tggaagttta caacgtcatc
     4021 tcaaacatcc cacctctttt gattgttatt gtcttgactt actcaatcgg agctggattc
     4081 tggaatctga tttttgccat gagcgtaaca acatggattg gtattgcctt catgatccgt
     4141 gtgcaaatct tgcgctatcg tgacttggaa tacaacttgg cgtcacgtac tttgggaaca
     4201 ccaaccttga agattgttgc caaaaatatc atgcctcaat tggtatctgt tattgtgaca
     4261 accatgactc aaatgcttcc aagctttatc tcatacgaag ccttcttgtc tttcttcggt
     4321 cttggattac cgattacagt gccaagtttg ggtcgtttga tttcggatta ttcacaaaac
     4381 gtaacaacca atgcttactt gttctggatt ccattgacaa cccttgtctt ggtatccttg
     4441 tcccttttcg tagttggtca aaacttagcg gatgctagtg atccacgtac acatagatag
     4501 gagtagaaat gacaaaagaa aaaaatgtaa ttttgactgc tcgcgatatt gtcgtggaat
     4561 ttgacgttcg tgacaaagta ttgacagcca ttcgcggcgt ttcccttgaa ctagtcgaag
     4621 gagaagtatt agccttggta ggtgagtcag gatcaggtaa atctgttttg acaaagacct
     4681 tcacaggtat gctcgaagaa aatggtcgta ttgcccaagg tagtattgac taccgtggtc
     4741 aggacttgac agctttatct tctcacaagg attgggaaca aattcgtggt gctaagattg
     4801 cgactatctt ccaggaccca atgactagtt tggaccccat taaaacaatt ggtagtcaga
     4861 ttacagaagt tattgtaaaa caccaaggaa aaacagctaa agaagcgaaa gaattggcca
     4921 ttgactacat gaataaggtt ggcattccag acgcagatag acgttttaat gaatacccat
     4981 tccaatattc tggaggaatg cgtcaacgta tcgttattgc gattgccctt gcctgccgac
     5041 ctgatgtctt gatctgtgat gagccaacaa ctgccttgga tgtaactatt caagctcaga
     5101 ttattgattt gctaaaatct ttacaaaacg agtatcattt cacaacaatc tttattaccc
     5161 acgaccttgg tgtggtggca agtattgcgg ataaggtagc ggttatgtat gcaggagaaa
     5221 tcgttgagta tggaacggtt gaggaagtct tctatgaccc ttgccatcca tatacatgga
     5281 gtctcttgtc tagcttgcct cagcttgctg atgataaagg ggatctttac tcaatcccag
     5341 gaacacctcc gtcactttat actgacctga aaggggatgc ttttgccttg cgttctgact
     5401 acgcaatgca gattgacttc gaacaaaaag ctcctcaatt ctcagtatca gagacacatt
     5461 gggctaaaac ttggcttctt catgaggatg ctccaaaagt agaaaaacca gctgtgattg
     5521 caaatctcca tgataagatc cgtgaaaaaa tgggatttgc ccatctggct gactaggagg
     5581 aaggaaatgt ctgaaaaatt agtagaaatt aaagatttag aaatttcctt cggtgaagga
     5641 agtaagaagt ttgtcgcggt taaaaatgct aacttcttta tcaacaaggg agaaactttc
     5701 tcgcttgtag gtgagtccgg tagtgggaaa acaactattg gtcgtgctat catcggtcta
     5761 aatgatacaa gtaatggaga tatcattttt gatggtcaaa agattaatgg taagaaatcg
     5821 cgtgaacaag ctgcggaatt gattcgtcga atccagatga ttttccaaga ccctgccgca
     5881 agtttgaatg aacgtgcgac tgttgattat attatttctg aaggtcttta caatcaccgt
     5941 ttatttaagg atgaagaaga acgtaaagag aaagttcaaa atattatccg tgaagtaggt
     6001 cttcttgctg agcacttgac tcgttaccct catgaattct caggcggtca acgtcaacgt
     6061 atcggtattg cccgtgcctt ggtcatgcaa ccagactttg ttattgcaga tgagccaatt
     6121 tcagccttgg acgtttctgt acgtgcccaa gtcttgaact tgctcaaaaa attccaaaaa
     6181 gagctcggcc tgacctatct cttcatcgcc catgacttgt cggttgttcg ctttatttca
     6241 gatcgtatcg cagttattta caagggtgtt attgtagagg ttgcagaaac agaagaattg
     6301 tttaacaatc caattcaccc atatactcaa gccttgcttt cagcggtacc aatcccagat
     6361 ccaatcttgg aacgtaagaa ggtcttgaag gtttacgatc caagtcaaca cgactatgag
     6421 actgataagc catctatggt agaaatccgt ccaggtcact atgtttgggc gaaccaagcc
     6481 gaattggcac gttatcaaaa aggactaaac tagtaatggt tttataattt ccatgtcaat
     6541 ttttatggaa attaggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6601 aggacaaatt tcgtcctttc ttttttgatg ttcaaagcga taaaaatccg ttttttgaag
     6661 ttttcaaagt ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc
     6721 gcttccagtt tggcattaga atagtgtagt tgaagggcgt tgataatctt tt
//
LOCUS       DAVHIU010000093         6713 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVHIU010000093 DAVHIU010000000
VERSION     DAVHIU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.095"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1755
                     /locus_tag="VVN06_001625"
     CDS             <1..1755
                     /locus_tag="VVN06_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU5563124.1"
                     /translation="MLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTA
                     MWALSGNFDASKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINIS
                     GDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAY
                     AKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKI
                     YNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTS
                     GVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNK
                     DGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSG
                     EEVFRRHFDKVVDGLLSLKVPSVRAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSN
                     IALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEK
                     LIIYNRTDAEIQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISL
                     RNAGIPLSLAEVEVYTYK"
     gene            1812..3578
                     /locus_tag="VVN06_001626"
     CDS             1812..3578
                     /locus_tag="VVN06_001626"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU5563125.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            3724..4875
                     /locus_tag="VVN06_001627"
     CDS             3724..4875
                     /locus_tag="VVN06_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU5563126.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGYDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            5020..5904
                     /locus_tag="VVN06_001628"
     CDS             5020..5904
                     /locus_tag="VVN06_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5563127.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(6021..6098)
                     /locus_tag="VVN06_001629"
                     /pseudo
     CDS             complement(6021..6098)
                     /locus_tag="VVN06_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            6302..6553
                     /locus_tag="VVN06_001630"
     CDS             6302..6553
                     /locus_tag="VVN06_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5563128.1"
                     /translation="MKEFQFERKQRFSLRKYVIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEY"
     gene            6541..>6713
                     /locus_tag="VVN06_001631"
     CDS             6541..>6713
                     /locus_tag="VVN06_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563129.1"
                     /translation="MGVLGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEK"
BASE COUNT         2164 a         1114 c         1465 g         1970 t
ORIGIN      
        1 atgttgccta tgtatactaa taatacaaag tcgttatcgt tagatttgac gccacatact
       61 tacgctgttg ttaaagaaaa tccaaataat ttacatattt tattgaacaa ttacaggaca
      121 gataagacag ctatgtgggc attatcagga aattttgatg catcaaaaag ttggaagaaa
      181 gaagaattag agttagcgaa ctggataagc aaaaattatt ccatcaatcc tgtagataat
      241 gactttagga caacaacact tacattaaaa gggcatactg gtcataaacc tcagataaat
      301 ataagtggcg ataaaaatca ttatacttat acagaaaatt gggatgagaa tacccatgtt
      361 tataccatta cggttaatca taatggaatg gtagagatgt ctataaatac tgaagggaca
      421 ggcccagtcc ctttcccaac accagataaa tttaatgatg gtaatttgaa tatagcatat
      481 gcaaaaccaa caacacaaag ttctgtagat tacaatggag accctaatag agctgtggat
      541 ggtaacagaa atggtaattt taactctggt tcggtaacac acactagggc agataatccc
      601 tcttggtggg aagtcgattt gaaaaaaatg gataaagttg ggcttgttaa aatttataat
      661 cgcacagatg ctgagactca acgtctatct aattttgatg tgattctata tgacaataat
      721 agaaacgaag ttgctaagaa acatgttaat aatttgtcgg gtgaatctgt tagtctagat
      781 ttcaaagaaa aaggagcaag gtatattaaa gttaaattac taacgagtgg agtgcctttg
      841 agtttagcag aagtagaggt ttttagagaa tcagatggta agcaatctga agaggatata
      901 gataaaataa cagaagataa agtagtctct acaaataagg tagctactca aagttcaacc
      961 aattatgagg gtgtagctgc tttagcagtt gatggtaata aagatggaga ttacggacat
     1021 cattcggtga ctcatactaa ggcagattct aacgcttggt ggcaggtcga tctgggagaa
     1081 gagtttacgg tttctaaagt tgatatttat aatagaacag atgccgaacc tcagcgttta
     1141 tctaattttg atgttatttt tctatcttca tcaggagaag aagtttttag aagacatttt
     1201 gataaagtag ttgatggttt gttatcttta aaagtacctt ctgtaagggc taagctagtc
     1261 aaaatagaat taaaatcagc agctattccg ttaagtttag cggaagttga agtctatggt
     1321 tcaaagagaa ctccgaagaa actttctaat attgcattaa ccaaagaaac tcgacagagt
     1381 tcaacggatt acaatggttt ttctcgtcta gcagttgatg gaaataaaaa cggagattat
     1441 ggtcatcatt cagtgactca taccaaagga gattctcctt catggtggga gatagattta
     1501 gcacaaaccg aagaattaga aaagttaatt atttataata gaacagatgc tgaaattcag
     1561 agattatcaa attttgatat tattatatat gattcaaata atcatgaagt ttttaaacaa
     1621 catattgaca gtttagaaag caataatcta tccatagact taaaagggct gaagggaaaa
     1681 aaggttagaa tttctttgag aaacgcagga attcctttaa gtttggcaga ggtagaggtt
     1741 tatacttata agtaatttta aaaattatca tcggtagtat gaaagaaaca gaaaaataag
     1801 aggaaaatag tatgattcaa catccacgta ttgggattcg tccgactatt gatggtcgtc
     1861 gtcaaggtgt acgcgaatca cttgaagtgc aaacaatgaa catggctaaa agtgtggcag
     1921 atttgatttc aagcacattg aaatatccag atggggaacc tgtggaatgc gtgatttctc
     1981 catctactat tggccgtgta ccagaggctg cagcttccca tgagttgttt aaaaaatcaa
     2041 atgtttgcgc aacaattaca gttacaccat gctggtgtta tggtagtgaa actatggata
     2101 tgtctccaga tattcctcat gctatttggg gatttaatgg gacagaacgc ccaggagctg
     2161 tctatcttgc agctgtacta gcttcacatg ctcaaaaagg gattccagcc tttgggattt
     2221 atggtagaga cgtccaggaa gctagtgata cagctattcc agaagatgtc aaagaaaaac
     2281 ttttacgtta tgcgcgtgca gctcttgcaa ctggcttgat gagagacact gcttacctat
     2341 caatgggtag tgtttcgatg gggattggcg gttctattgt aaatccggat ttcttccaag
     2401 aatacttagg aatgcgaaat gaatcggtag atatgacgga gttcacgcgc cgtatggacc
     2461 gtggtattta cgaccctgaa gagtttgaac gtgctatggt ttgggtaaaa gaacatatca
     2521 aagaaggagt tgaccgaaat cgtgaggatt tgattctttc taaagaagag aaagaaaaac
     2581 aatgggaatt tgttattaag atgttcatga ttggacgtga cttaatggtt ggtaacccaa
     2641 gacttgctga acttggtttt gaggaagaag cagttggtca ccatgcttta gtagctggtt
     2701 tccaaggtca acgtcagtgg acagaccatt tcccaaatgg ggactttatg gaaactttcc
     2761 tcaatactca gtttgactgg aatggtattc gaaaaccatt tgtatttgcg acagagaatg
     2821 attcactaaa tggtgtgtct atgctcttta attatctatt aacaaatact ccacaaatct
     2881 ttgctgatgt gcgcacttat tggagtccag aggctgttaa acgtgtaacg ggacatactt
     2941 tagagggttg tgctgcagct ggcttcttac atctaatcaa ctctggttct tgtacattgg
     3001 atggtacagg gcaagctact cgagatggca aacctgttat gaaacccttc tgggagttgg
     3061 atgaaagtga agtgcaggct atgcttgaaa atacagactt cccaccagca aaccgcgaat
     3121 acttccgtgg aggaggattc tcaactcgtt tcttgacgaa gggggatatg ccagtaacaa
     3181 tggtacgtct caatcttcta aaaggggttg gtccagtgct acaaattgca gaaggttata
     3241 cacttgaact tcctgaagat gttcaccata ctttagataa tcgtacagat ccaggatggc
     3301 caactacttg gtttgctcca cgtttgacag gaaaaggtgc tttcaagtct gtctatgacg
     3361 tcatgaataa ttggggagct aatcacggag ccataacata tggccacatt ggagcagact
     3421 tgattacctt ggcttctatg ttgagaattc ctgtcaatat gcataatgta cccgaggaag
     3481 atatctttag acctaaaaat tggtccttat ttggaacaga agatctagaa tcagcagact
     3541 atcgtgcatg tcagttgttg ggaccgctac ataaataaaa attgtgtata taggaggtga
     3601 acttatgtcc ctcctatcct tttaaaaaga tttgttaaac aattcacaaa taattgaaaa
     3661 cgaatacaaa aactaatata atgatgttaa atagatagcg cggaggcgca ggaggaaaat
     3721 tatatggcta cattttatgt tccggcagtc aaccttattg gtaaaggtgt tgtaaatgaa
     3781 gtgggtcctt atatcaagga acttggctat aaaaaggcac ttttggtgac agataagtac
     3841 atcgaaggca gtgacatttt acctaaggtt ttaaaaccac tggatacaga aggaatcgaa
     3901 tatgtcatct ttagcgatgt agagccaaac cctacttgta agaatgtcac agatggggta
     3961 gctgctttgc aagaacatgg atgtgatttt atcatcagtc ttggtggggg ttctccacag
     4021 gatgcagcta gttgtatttc tatcatggct acaaatggtg gaaaaccaca ggattatgaa
     4081 gggcttcata agtctgctaa aaaaggcttg ccagttgtgg ctatcaatac aacggcagga
     4141 acttctgcag aaatcactat taactatgtg attactgatg aagaacgcaa ggttaagatg
     4201 gtaatggttg acaagaatag ccttgctctt atctctgtta atgacccgga actcatgctt
     4261 tccaaaccta aaggcctgac tgctgctact ggtatggatg ctctgactca cgctgttgaa
     4321 gctttggtaa cacctggtgc ttatgatgta accaagaaac tgtctattgg tgctattgag
     4381 cttatcaagg aatatcttcc tcgtgctgta gaaaatggat acgatattga agcgcgtgaa
     4441 ggtatggtca atgccatctt ccttggtggt atgagcttta ataatgctgg tcttggttat
     4501 gttcactcaa tggctcacca actcggtgca gtatataatt tgccacatgg tgtttgctgt
     4561 gccatgttgc taccagttat agaacgtgaa aatgctaaac gtgtaccaga agctttccgc
     4621 aatgttgcca aagccttggg ccttcatgtg gaaggtaaat cagatcaaga gtgtgccgat
     4681 tatgcgattg ctgagattga gaagctttct gagacagtag gtattcctaa gaaattgact
     4741 gaacttggta ttgaagaaaa agatttcgac tttgaatacc tttctaagaa tgccttgatt
     4801 gatgcctgcg caccaggaaa tccatttatg ccaaccttag aagaaacgat tgccttttat
     4861 aaagagttat tttagcaaga gattagacga caataggaca attcacctct aattattgac
     4921 atttcaggaa aaatcgcagt aaaaacggca cagccttctt taaatgtgct ataatactag
     4981 aaaaatactt gtggaggttc cattatggca atatttttca tgatttttct gattgtttgt
     5041 gtgctcctat tggtgatagt cacactgagt acagtttatg tggttcgtca gcagtcggtg
     5101 gcgattattg aacgctttgg gaaataccaa aaggttgcta atagcggtat tcatattcgc
     5161 ttgccttttg ggattgactc gattgcagca cggattcagt tgcgcttgtt gcaaagtgat
     5221 attgtggttg agactaagac caaggacaat gtgttcgtta tgatgaatgt agcgactcag
     5281 taccgtgtca acgagcagag cgtgacagat gcttactata aactcatacg tccagaatct
     5341 cagattaaat cttatatcga agatgctctt cgctcttctg ttccaaaatt aaccttggat
     5401 gaattgtttg agaaaaaaga tgagattgcc cttgaggttc aacaccaagt agcagaagaa
     5461 atgaccactt acggctacat tatcgtgaaa accttgatta ccaaggtcga accagatgca
     5521 gaagttaagc aatctatgaa tgaaatcaat gcggcgcaac gtaagcgggt cgcagcacaa
     5581 gaattggcgg aagctgacaa gattaaaatt gtcactgcag ctgaagcaga agcagaaaaa
     5641 gaccgccttc atggtgtggg gattgctcaa caacgtaagg cgattgtgga tggattggca
     5701 gagtctatca ccgaactcaa ggaagccaat gttggcatga cagaagaaca aatcatgtct
     5761 atcctcttga ccaaccagta tttggatacc ttgaatacct ttgcctctaa aggaaatcaa
     5821 accatctttt taccaaatac gccaaatggt gtggatgata tccgaacaca aatcttgtca
     5881 gcccttcgcg ctgagaagaa ataatagact aatactcttc gaaaatctct tcaaactacg
     5941 tcagcgtcgc cttgccgtat atttgagctg acttcgtcag ttctatctgc aacctcaaaa
     6001 cagtgttttg agctgacttc gtcagtctta tctactacct caaaacggtg ttttgagcaa
     6061 cctgtggcta gtttcctagt ttgctctttg attttcattg agtataaaga gcttggcaac
     6121 aggctctttt tgtattgcgt ttattaagaa aaacaaaaaa catcgatata catttaacta
     6181 aaaattattg tatgttatct cccttaatta ggaatgataa gggaataact agaaagattt
     6241 gtgaatacaa actatttctg atatacaaaa tatacagtaa taatgaatga tgggagatgg
     6301 gatgaaagaa tttcaatttg agagaaagca gcgtttttct ttgaggaaat atgtaatagg
     6361 agcttgttcg gtcttgctag gaacgagttt attttttgct ggtatgggtg ctcagcctgt
     6421 acaggataca gaaacgagtt cagcactaat ttcaagtcat tatttggatg agcaggattt
     6481 atctgaaaag ctgaaatctg agttgcaatg gtttgaatta gaaaacaagc ttttgaactt
     6541 atgggagtat taggttacta tgaaggattt gttccttatg tttcaaatca atacaaaaac
     6601 caagctgaag aagaaggcaa accgctatct gataaatata ttttcgaaaa aatcttagga
     6661 aaaacatatg cagccttcaa aaaagatcaa attaatgagc gtgtcgagaa att
//
LOCUS       DAVHIU010000094         6702 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVHIU010000094 DAVHIU010000000
VERSION     DAVHIU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.096"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..2214
                     /locus_tag="VVN06_001632"
     CDS             <1..2214
                     /locus_tag="VVN06_001632"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU5563130.1"
                     /translation="KKCGFDLQNTANHKDSLETFLRRFFFTYQDTNYPLSILAADKKT
                     DLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDVFRKETGFPIIYGDLIENLYQ
                     LLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLATFSNQDVIREVVYVESRVDTD
                     QKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKASDKALYKMEGELEVKLPHKI
                     ELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDYFLPRGFANLYVSGVGTKDST
                     GFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVKADWSNGKVATTGLSYLGTMS
                     NGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGGYPGEDFDSLAELTYSRNLLA
                     GDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYLLNAHKVKAEVVFTHGSQDWN
                     VKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSIDFRESINALLTKKLLGQETDF
                     QLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQAIQNQYPDKDFERYGKTYQT
                     FNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSSTNKGLLSAQLLEFGQKKYLQ
                     PYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKGYLNLQNRNDLLLVEDITADE
                     WMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNTDYHLTVDLAQSMLTLPC"
     gene            2231..2701
                     /locus_tag="VVN06_001633"
     CDS             2231..2701
                     /locus_tag="VVN06_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5563131.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(2818..2973)
                     /locus_tag="VVN06_001634"
     CDS             complement(2818..2973)
                     /locus_tag="VVN06_001634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563132.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKATLTRIEEI
                     FEEYKSS"
     gene            complement(3244..6594)
                     /locus_tag="VVN06_001635"
     CDS             complement(3244..6594)
                     /locus_tag="VVN06_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5563133.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLEQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6624..>6702)
                     /locus_tag="VVN06_001636"
     CDS             complement(6624..>6702)
                     /locus_tag="VVN06_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU5563134.1"
                     /translation="LVDKSQFACEIAIKVWRNSLKFSII"
BASE COUNT         2073 a         1459 c         1141 g         2029 t
ORIGIN      
        1 aaaaaatgtg gctttgattt acaaaataca gccaatcaca aagactcact tgaaaccttt
       61 ttacgtcgtt tctttttcac ttatcaagac accaactacc cactttctat cctagcagct
      121 gataaaaaaa cagacctgct gacatttttt caatcagaag atgaactgac agcagatatt
      181 ttttatactg ttgcttttca acttttaggc ttttcttatt tggttgactt cgaagatagt
      241 gacgtttttc gtaaagaaac tggatttccc attatatatg gtgacttgat tgaaaatctc
      301 tatcagttac tcaatactcg caccaaaaaa ggaaatactc ttatcgacca acttgttagt
      361 gatggtctta ttccggagga taatgactac cactacttta acggcaagag tttggctact
      421 ttttctaacc aggatgtcat tcgtgaagtc gtttacgttg agtctcgtgt cgatactgac
      481 caaaaagggc tatcagacct agtaaaggtt agcattattc gccctcgttt tgatggaaaa
      541 atccctgcca tcatgacagc cagcccttat catcagggaa ccaatgacaa ggctagtgac
      601 aaggctctct acaagatgga gggcgagctt gaggttaagc ttcctcacaa gattgagcta
      661 gagaaacctc aactaaatct cgtccaaccc caaggtaaag ctgagcttat agcagaggct
      721 gaggaaaagc taactcacat caactctagc tatacactaa acgactactt ccttccgaga
      781 ggctttgcca atctctatgt ctcaggtgtt ggtaccaaag actcgacagg tttcatgact
      841 aatggagact accagcaaat cgaggcttat aaaaacgtca tcgattggct taacggtcgt
      901 tgccgtgcct ttactgatca cacgcgccag cgtcaagtca aggctgattg gtcaaacgga
      961 aaagttgcta caacaggact ttcctatcta ggtaccatgt ccaatggtct tgcgactaca
     1021 ggagtcgatg gtttagaagt tatcattgcc gaggcaggaa tttcatcatg gtacaactac
     1081 taccgtgaaa acggtctggt gactagccca ggtggttatc caggtgagga ctttgactcc
     1141 cttgctgagt taacctattc tcgtaatctc ttagctggcg actatatccg tggcaatgaa
     1201 gctcaccaag ctgacttaga aaaagtgaaa gctcagctag accgaaaaac tggggactac
     1261 aatcagtttt ggcatgaccg caattatctg ctcaatgccc ataaagtaaa ggcagaggtt
     1321 gtctttactc atggttctca ggattggaat gtcaaaccac ttcatgttta ccagatgttc
     1381 catgctcttc ctactcatat acacaagcat ctctttttcc ataatggtgc ccatgtttac
     1441 atgaacaatt ggcaatcaat tgacttccgt gagtccataa atgccttatt gaccaagaaa
     1501 ttactaggac aggaaacaga ctttcaactt cctactgtta tctggcagga caatacagct
     1561 ccacagactt ggttatcact tgataacttc ggtgggcaag aaaactgtga aaccttctca
     1621 cttggtcaag aagagcaagc tattcaaaac cagtacccag ataaggattt tgagcgctat
     1681 ggtaagacat accaaacctt caatacagag ctctatcaag ggaaagctaa tcagattact
     1741 attaaccttc ctgtgactaa agatctccac ctaaacggtc gcgctcagct caatcttcgt
     1801 atcaaatcca gtacaaacaa ggggctctta tctgcccaac tgctagaatt tgggcaaaag
     1861 aaatacctac aaccttatcc agctatttta agtgctagaa ccattgacaa cggtcgctat
     1921 cacatgttgg aaaatctctg tgaattgcca tttagaccag aggcacaacg agtcgtgaca
     1981 aaaggttacc ttaatttaca aaatagaaat gatttactgt tagtagagga tattactgca
     2041 gatgaatgga tggacgttca atttgaactg cagccaacta tttacaagct aaaagaagga
     2101 gacactctcc gtttagtcct ctatactact gactttgaaa tcaccatccg tgacaataca
     2161 gactaccact tgactgtcga cctcgctcag tccatgctta ccttaccttg ctaaaaggag
     2221 ttacattatt atgaataaat cagaacaccg ccaccaactt atacgcgctc ttatcacaaa
     2281 aaacaagatt catacacagg ctgagttgca agcccttctt gctaagaacg acattcaagt
     2341 aacccaggca accctctcac gcgacatcaa aaatatgaac ctatcaaaag tccgcgaaga
     2401 agatagcgct tattatgttc ttaacaatgg ttccatctca aaatgggaaa aacgtctcga
     2461 actctacatg gaagacgccc ttgtctggat gcgcccagtt caacaccaag tcctactaaa
     2521 aacccttcct ggactggctc aatcctttgg ttctatcatt gatactttga gcttccctga
     2581 cgctatcgct accctttgtg gtaatgatgt ctgtcttatc atctgtgaag atgcagatac
     2641 tgctcaaaag tgctttgaag aactgaaaaa attcgcccca ccatttttct ttgaagaata
     2701 ataaatgact acaactaaca attatgcttt taaaaaacct ggtagaaagt ctttaacatc
     2761 agaaaacgca tagtatcaag tgttgaaaac ccttgatact atgcgttttg ctttatccta
     2821 ggaagattta tactcttcga aaatctcttc aatccgcgtc aacgtcgcct tgccgtaggt
     2881 atggttactg acttcgtcag tttcatctac aacctcaaaa cagtgttttg agcagcctgc
     2941 agctagtttc ctagtttgct ctttgatttt cattgagtat tatttcattt tctcctgcaa
     3001 ttgaattctt gctcagcttt ttgttttcta tttctttaaa atcaaagtag ctcttttgtt
     3061 aataactcga tcaacaaaca tcgtggtaca agtatctact ttgaaattta tcaaccactt
     3121 aacaactgat actgtattct aggaaaacga tgacattctt cctaataaaa cttctcatat
     3181 atagcataaa tttctactct ttttaatttt aaatgagtct cagcatggca atcactttag
     3241 aatttaagcc gcttctccag ctcgattatt gataagttca atgatttgaa caatattacg
     3301 aagtactggt agttggtgtt ggaagagtag ttgaacagaa ccataagatt taaacggctc
     3361 ttgcgttaac actttcgtct ctaaaaaacc attttctaca atgtagtcta caatcaattt
     3421 tacaaatgaa atctgcctgg cattaagatt ctcatccgac aaaaatttag aaaaaatacg
     3481 attggcagac tctctatcta agccaataat ctcacgaacc aatctcggaa ttgccttatt
     3541 ttcataatga ctttggtagt ctgctttact acctaatttt tcccaaagca atttttcaag
     3601 tgcaagcata tcatcagatg tcaacttttt attatggtat aacttagaaa tggactcctc
     3661 atccagatga gttttcaaat aatgctcaac tttttcattg taagaccgaa gatcgttgac
     3721 ctgcaaaaat gctgtggtct cgtgaacagt ggagagtata cgatcttcaa agttaacata
     3781 gtagggttta cggtctgttt tatccaaaaa ctgtaataaa tctcgaatag caagacgaat
     3841 tttttccaaa tcagacaagt taacttcttt ccaaaattca ggctcctgta ctttcctgat
     3901 aatttcagcc tgctcaaaaa cctgtgggat attgccaata gcagaaagag ctctagccgt
     3961 cttcatcact tgggaaatat gaacagtgga agatttagct gtcagttgct ccaactgaat
     4021 atgaagcaac cacaaatcaa atctcctcgc catctcatct tctttatctt catcaaataa
     4081 aagcggagag agattttttt gaatggtttc acttgtaaca gcagttagat tctgccaact
     4141 ttccaatttc ctatagctat aaactgtatc taaaaccata cgaaccctga agtccaactc
     4201 atttaagctc tctatacgac cttgaagttc cgagacaagc tgctgacggt atgctctcgc
     4261 aaactgatct tcttggtatt ggagtccctg aagttctcga atcaagtcca ctttgatatt
     4321 aaataaacgc tgagtcagcg aaacaatgtg acgaccctct ccatctcttg gatctgcacg
     4381 aaaataatca aaattgtccc cataatcaaa taccaagaag ttttccttat cctgctcagg
     4441 tccaaataaa tctttacata gacgggttcc tcgaccaatc atctgccaaa acttagtttt
     4501 agagcgtact ttcttgaaga agactaaatt aacaacctct ggtacatcaa tacctgtatc
     4561 taacatatcg acagaaatcg caatttgagg atacttctcc ttaattttaa aatcatcaat
     4621 caaggtctga taatgcttaa tactataatc aatcacctga gcatagtcgc tccctttttc
     4681 aggatagcgg ttgttaaaaa tacctctgat atattccgca tgatcatgat ttttagcaaa
     4741 aataatagtt ttaccaattt catcacccga ggctgtctga attcctcttg tcatgagttc
     4801 atttaaaaca atttctactg tacttttatt gaaaataaag gaattaaata cactcccatc
     4861 aatatctttt tcacagctat tgtcttcaaa tttgctatca aaatgttcct tttcttcttc
     4921 ggacaaatca tcataatgta gaccatccgt aggtagtttc agtttggttt cgatagaatg
     4981 ataggctact aaatatccgt ctttaacagc ctcttccaaa tcatatgcat atgttggaac
     5041 cccattctcc aaattaaaga atccataggt gtttttatct aaatcttgac gcggagtagc
     5101 tgttaagcct acaattcttg catcaaaata atcaaaaatg gacttgtatt tctgataaat
     5161 agaacggtga gattcgtcaa ttatgataag gtcaaaatgc ccaacagtaa aagggcgttg
     5221 atttacttct tcttgaccac taatcgctcc aatcatggtc ggataagttg aaaagaccat
     5281 acgacttgat tgagctcctt ctttatcttc taagaagtta caaacggaaa gatctgggag
     5341 taattttcta aacgaatcat aggcttgctt aaccaaggaa gttctatcgg ctaagaagag
     5401 aacgtttttt acccagttat gacgtgataa gatatcaact agagaaactg ctgtacgagt
     5461 tttccccgcc ccagttgcca taaccaaaag tgcctgtcta cgatgattag agaaagcttc
     5521 acaaacgctt gcaatggcat gtttttggta gtgacgcccg gaaatatcgt ccctaatttt
     5581 actagaaata tcctcaagcg gtttttgaag atgacgtcta tccatcacta attgcaattc
     5641 ttcttgagag taaaagcctg caatctggcg gcggttcgga ccatcaagta tatagtgctt
     5701 caacccattt gtaataaagc aaattggctg atagccgata tgtttctcaa gagcttcagc
     5761 atattctttg acctgtactt cccctacttc tggattgaca gaggcttttt tagcctccac
     5821 aatcgctaaa attttcccat ttttaccata aagtacatag tcacagtaac caattcctga
     5881 accgtgcttg agaccatcaa cggctatctc aacacgacag ttttcttcaa atatccatcc
     5941 tgctaaacgg agatcgatat caataaagag ctttcttgtc tctgcctcag ataattgttc
     6001 aatatggaaa ggcacatctt gccgttgcgc tgcagtctca cgtttttcag tataagtctc
     6061 ttgaacttct acggactgag cagccatctg ttcatgaaaa tcaggcagtt ctggtaaact
     6121 gtcttgtaac tttatcatag attgtggagt ttctcggtat ttgatgtttg ctgaaagtgg
     6181 taagcacttc tcatcaaaat aacgctccac aaattcattg ctgtaacaat aatcgataaa
     6241 attaacaaac tgatacaaat gatgtaaagc taaaatcgct tctcgttcct taatttcacc
     6301 accatgagca gcatggtttc cccaccgaat caacagaact atctgcttgt ggagttcaga
     6361 atctacgata tccctaaaat catcatccca tactaaagaa gatagagtag cacgataggg
     6421 agcttctaaa tatgaatcgt gactatatat ccaatggaca gcctgctcca aagcacgacg
     6481 cgccataaag gcagtagcca cagttgatgt agcaatcata ttttcagctt caatgcaagg
     6541 ttttgaaaaa gattcatatt cgtcattttt taaaagaaag ctaaaatttc ccattttttt
     6601 ctcactttaa acaaatagtt tagctatatt atactaaatt tcaagctatt tctccacact
     6661 tttatagcta tctcacaagc aaattgtgat ttgtcgacca gg
//
LOCUS       DAVHIU010000095         6540 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVHIU010000095 DAVHIU010000000
VERSION     DAVHIU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.097"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VVN06_001637"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VVN06_001637"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5563135.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VVN06_001638"
     CDS             1377..1886
                     /locus_tag="VVN06_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563136.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GIGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VVN06_001639"
     CDS             1963..4368
                     /locus_tag="VVN06_001639"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5563137.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENTVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVIIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQVSLEEKVNA
                     EVR"
     gene            complement(4436..5437)
                     /locus_tag="VVN06_001640"
     CDS             complement(4436..5437)
                     /locus_tag="VVN06_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5563138.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            5584..5990
                     /locus_tag="VVN06_001641"
                     /pseudo
     CDS             5584..5990
                     /locus_tag="VVN06_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6113..6379
                     /locus_tag="VVN06_001642"
     CDS             6113..6379
                     /locus_tag="VVN06_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5563139.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
BASE COUNT         2005 a         1209 c         1496 g         1830 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtagag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggagccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggtt ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggaatagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtaga gagtgtcgaa tcaccagctg ctgatctctc aaaaattgtt
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagaagc ccgtcagatc gtttgtggtg ccccaaatgt gcgtgctggg
     2221 atcaaggtca tggtggctct tccaggagct cgtatcgctg ataactacaa aatcaaaaaa
     2281 ggaaaaatcc gtggtttgga gtcacttgga atgatctgtt cacttggtga attgggaatt
     2341 tctgactcag ttgtgcctaa ggaattcgca gatggcatcc aaatcttgcc agaaaatacc
     2401 gtgccaggtg aggaagtctt ttcttaccta gacttggatg atgaaatcat cgaactttcc
     2461 atcacaccaa accgtgcaga tgccctttct atgtgtggag tggctcacga agtggcagcc
     2521 atctatgaca aggcagtcaa ctttaaagaa tttactctaa cagaaactaa tgaagctgcg
     2581 gcagatgccc tttctgtcag cattgggaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgattatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct ctactttggt
     2761 caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt tttcaatggt
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt cagaatcgtc ttctcgcttt
     3061 gaaaaaggca tcaatgtggc aacagtcaat gaagcccttg atgcggcagc tagcatgatt
     3121 gcagaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatacc
     3181 tctgatgtgg aagtttcttc aacccttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggtt ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga agctgacctc
     3361 tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caactagtct accaaaagac
     3421 gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt tcgtaccatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatgctc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttac gagattggaa aagtctttga acaaacaggt
     3721 aatccaagag aagaacttcc aaatgaaatt aacagttttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaggga
     3841 atccttgaag ccctatttac tcgcttggga ctccaagtaa cctatacagc aacatctgaa
     3901 atcgctagcc ttcatccagg tcgtacagca gtcatttcac tcggtgacca agttcttggt
     3961 ttccttggcc aagtgcatcc agtcactgct aaggcttatg atattccaga aacgtatgta
     4021 gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc atttgtagaa
     4081 atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc agaagtgact
     4141 catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agatagctta acggacgaag aagtcgcacg ctatatggaa
     4321 aaaatccaag tatcgctcga agaaaaagtg aatgcagaag tgcgttaaac gatagtatga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca atgtctaaat
     4501 ctctaacata gtagtggttt ttaggcaata agatatgatc tatcgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg aagtgcattc aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccactattgg
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc atcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata gggaaaaaaa tacgaaactt aaaatagaag
     5281 ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt ctggtagata
     5341 gccaaatatt aagattagaa gatggaaaaa aagaataagc tatgataggg ataacaaaga
     5401 gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct caatttgcaa
     5461 tggttgctcg gagctaatgt gactttactt gattgatatt ctttttcttg tcttcctcat
     5521 agattaagta caacccaagt aaaaaagttc aatttaagat tgaaatgatt aaaaggcaca
     5581 gaaatgcaag acaattatac tacaaaaggc aaacatttga caatcgatag ccgtcgctta
     5641 atcgaacgat ggaaaaaaga aggaaaatca aatagagaaa ttgcctctct acttggaaaa
     5701 gctcctcaaa ctatccacac tgaaatcaag cgtgggacag tccgacaatg tcttggaaaa
     5761 gggcgcttca aagagattta ttctgccgac tacgctcaac agtcttaaga aactaaagga
     5821 aaagattctc cactatacat aaccaaaaat tttcgcctga aatgatggtg atggctaaag
     5881 gggttaacgt gggaatttca accatttact attgaattca tcgtggaaaa ttggggttaa
     5941 gcaagcagga tctgctttat cctagaaaag gaaaatcgct taagaaatag gctagcacca
     6001 actttaaacc cgctggtcaa tctatcgaat cataattcgc tcattttttt aaaaatacat
     6061 agacgtaaaa aaaagagggt taagtgatga agaatagtgc tattgggagt aattggaagg
     6121 atgtccatct gagctcttta gcaaggagga aatccttgaa agtgatatgc gagtggctat
     6181 catgagcgag ttgattgagg ctaggtatga acaaggaatc agtcagaaaa agctggaaga
     6241 agtcagtgga ataagccagc ctgtcatagc taggatggag acaggagaga gcagtcttca
     6301 gttggatacg gtcttaaaag ttctaaccag tctaggaaag acactagcag tcgttccact
     6361 tgaacagggg aaaagttgac tagaatcagt tatagtcttt tctaataaca ggtcatagtc
     6421 acttataaga attactaata acatgtttta gtattctaat tggaatatag ctgtaaagta
     6481 agaattacaa aggatttgag atgtttgctt caagtctttt tcttttgtcc gaaacagata
//
LOCUS       DAVHIU010000096         6524 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVHIU010000096 DAVHIU010000000
VERSION     DAVHIU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.098"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(63..2321)
                     /locus_tag="VVN06_001643"
     CDS             complement(63..2321)
                     /locus_tag="VVN06_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5563140.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2550..2780)
                     /locus_tag="VVN06_001644"
     CDS             complement(2550..2780)
                     /locus_tag="VVN06_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU5563141.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            2970..3653
                     /locus_tag="VVN06_001645"
     CDS             2970..3653
                     /locus_tag="VVN06_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5563142.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3653..4387
                     /locus_tag="VVN06_001646"
     CDS             3653..4387
                     /locus_tag="VVN06_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5563143.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKVDAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4541..5398
                     /locus_tag="VVN06_001647"
     CDS             4541..5398
                     /locus_tag="VVN06_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU5563144.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5540..6412
                     /locus_tag="VVN06_001648"
     CDS             5540..6412
                     /locus_tag="VVN06_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5563145.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENILALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERARKDKLV"
BASE COUNT         1833 a         1226 c         1464 g         2001 t
ORIGIN      
        1 acagttattt tagtaaaaaa agaagcagaa aaatttcatt ttctactcct tttctatagg
       61 atttattttt ctttttcaga acttttaact tcagcttttt tggcagattt aatctgaatg
      121 tttcccttgc tagtcataac tgctttgaga tctttttcgc ttggattttc aaggtagtag
      181 tcagtgattg tgtcctcaat atagtcttga atagtccgac gaagtgggcg tgctcccatt
      241 tttggatcat aacctaggtc aaccaacttt tccttgacct tatcagttac atccaaacga
      301 atgttgttgc tagagaggcg cttgttaaca tctgctagca tgagctcgac aatctgaagg
      361 aggttatcct tgctgagagc cttaaattcg ataatgccat caaaacggtt cataaactct
      421 gggctaaaga agttaccgag ttcaccgagg acagaattgg tacgtccttc tctagcagca
      481 ccaaatccaa cgctagcttc ggtctttcct gtacctgcat ttgaggtcat gataatgatg
      541 gcatccttga agctaacggt gcgtccttgc ccgtctgtca aacgaccatc gtccaagact
      601 tgaagaaaca tgtgcataac atctgggtga gctttttcca cttcatcgag aaggatgaga
      661 gaatatggat tgtggcgaac tttttcagtt aattgaccag cctcatcata gccaacataa
      721 cctggagggg cgccgaccaa cttagccaca ctatgttttt ccatgtattc actcatatca
      781 aagcgaatca tactatcagc agaaccaaaa agttcgatag ccagttgttt ggaaagttct
      841 gtcttaccga caccagttgg cccaacgaag aggaagcttc cgattgggcg gttaggggta
      901 ccaagtccga cacgattacg gcgaatagcc ttggcaatct tatcgactgc atcatcttgg
      961 ccaataacat gagacttgag atcttcggct agatggatga gttgagattg ttctttctct
     1021 ttcaaatcac caacagggat attggttttc tgctcgataa tgtgctcaat agttttctcg
     1081 ctgatgatag gagtatcctg gtctgtgatc tttttctttt gcatttcctt atacttggca
     1141 atctggtcgc ggaagtaggc cgccttctca aaatcttctt ctcgtgtagc ttgagacttg
     1201 agattttcag cctcaatcaa gcgctgatca attactttag gatccacaaa attcaaggtc
     1261 aagttcatct tagaaccagc ttcatctagg aggtcaatgg ccttgtcagg caagaagcga
     1321 tcttggatgt agcgattgga aagagttgca gctgcttcaa tcgcagcatc tgtatattga
     1381 acgtggtggt aatcttcgta tttcttttga atccctttga gaatagtgat tgtttcgtcc
     1441 accgttggtt catcgacttt aacaggctgc atacgacgct cgagggcagc atccttttca
     1501 atgatacggt attcattgag ggtagtagca ccgactagtt gcagttctcc acgagcaagg
     1561 gctggcttga ggatatttcc tgcgtccata ttaccatcac tcgcagaacc agcaccaaca
     1621 atttcatgga tttcatcgat aaagaggatg atgtcttcac gtttgcgaat ttcttccatg
     1681 agtttttgca tgcgttcttc aaattgtcct cgaatccccg ttccttgaac taagctaacc
     1741 acatccagac ggatgacttg tttaccttgg agtttatgtg gcacatcgcc atcgacaatt
     1801 ttctgagcta gaccttcgac aacggccgtt tttcctacac caggttcacc gataaggaca
     1861 ggattattct tggttctacg attgagaatc tcgatgacac ggataatctc atcgtcgcgc
     1921 ccaataacgg ggtcaatgtc tccacgacgg gcaatttcag ttacattaat accaaattct
     1981 tccagcaggc ctttttcttg gctaggtggc ggagtttgag cagatccacg attttgggaa
     2041 ccataaccgc cgtttccacc gtaacctcca cctgattggg ttgggggaat aggcggagta
     2101 ttgctagaag gtctgaaatt gtttagatca ttgaagaaat caccaaaggg atcgaagtca
     2161 cgattgttca gatccgtcat acctttgaag aggctattgt taggatctgt tttgataatc
     2221 ttatagcagt tttgacagag gtcaatttgt ttttgttttc cattgagatt ggtgtaaaga
     2281 tgaattgttg agtcgttaat tttacagttt tgacaaagca tacatctacc tcatttcttt
     2341 ctttagcctg acttgtttaa aggtcaaaaa tagtcaaatt ctatactctc attataacag
     2401 gattggggtg taaagcaagt aaaaagattg aagctttgag aagttgttac aatgaaaatt
     2461 tttgtgaatt tggactataa aaaaatccta tttgtgagac aaaaaggatt ttacttagac
     2521 atgcagggac aatcccaact tgttttagat tagtagagaa gttcgtaaat ttccattgcg
     2581 attaagtcaa tgctatcaaa cgtatacgtt tcgcgatctt ctacgtcacg aagggtaaag
     2641 attgggccgt tttcttcgtc gtggctgtac gcaacttctg caacaacaac tccttcgcgt
     2701 tcaaaattac gttttaaatt tccgccatcg tttgccattg cttcaaggcg gttgatgatt
     2761 ctaaccaaat gtgattccat tttgattctc cttcatttct tatcgttact attttaccat
     2821 aaaggagggc aacttgacta gaaaaaacta agatttctcg ctaattctgc tagtttaaag
     2881 atttttaaat aaattggata aaaaatttaa attttaattc aaaacatgtt ataatcatac
     2941 agtattctat tttagaaagc agtgtgacta tgaatttttc ttttttacct aagtatttac
     3001 cttattttaa ctatggggct gttgtgacga ttcttatttc tatctgtgtt atctttttgg
     3061 gaactatttt gggtgttgtc ttggcttttg ggcaacgttc aaagtttaaa ccgcttgttt
     3121 ggttggccaa cttgtacgtt tggattttcc gtgggacacc gatgatggtt caaattatga
     3181 ttgcctttgc tcttatgcat atcaatgctc cgactattca gattggaatt ttaggtgttg
     3241 atttttcgcg tctgattcca gggattttga ttatctctat gaatagtggt gcttatgttt
     3301 cggagactgt tcgtgccgga atcaatgcgg ttccaaaagg tcagctagag gcggcttatt
     3361 cgctagggat tcgtcttaaa aatgcgatgc gttatgtgat tttgccacaa gcagtcaaaa
     3421 atatcttgcc agcattgggg aacgaattta tcaccattat caaggacagc tccctcttat
     3481 cagctattgg ggtcatggag ttgtggaatg gggctacaac agtttctaca acaacctatc
     3541 tacctttaac accactttta tttgcagcat tttactactt gattatgacc tctattctga
     3601 cagtagcctt gaaagctttt gaaaaacata tgggacaagg agataagaaa taatgacaga
     3661 aaccttgata aaaattgaaa atttacataa atcttttgga aagaatgaag tattgaaggg
     3721 catcaacctc gagattaaaa gaggagaagt tgtcgttatc atcggtcctt cagggagtgg
     3781 gaaatctacc ttgcttcgct ctatgaattt gttggaggaa gcaaccaagg ggaagcttat
     3841 ctttgaggga gtcgatatta cggacaagaa gaatgacctg tttgccatgc gtgagaagat
     3901 gggcatggtt ttccaacaat ttaacctctt tcctaatatg actgtgatgg aaaatattac
     3961 cttgtcacct atcaagacta aaggtgaaag cagggaagtt gctgagaaga gagcccaaga
     4021 gcttttggaa aaagttggtt tgccagataa ggtagacgct tatccacaga gtctgtcggg
     4081 tgggcagcaa caacggattg ccatcgcgcg tgggttggct atggaaccag atgttttgct
     4141 ctttgacgaa ccaacttcag ccttggatcc tgaaatggta ggtgaggtat tggctgttat
     4201 gcaagacctt gccaagtcag ggatgactat ggttatcgta acacatgaga tgggatttgc
     4261 ccgtgaggtg gcagatcgtg ttatctttat ggcagacggt gtggttgttg aagatgggac
     4321 acctgagcag atttttgaac aaacccaaga acaacggact aaagatttct tgagtaaggt
     4381 tttataatct attttaaaat ttcaagacaa gattagtgaa aagttcaacc agagcttttt
     4441 cttatagttt gaaactatag gataacctag gaaagaagtg ttagagggca ctagcagttt
     4501 ttggtataat ggaagatatt tgaaagaaaa gagaagtgat atgacacaga ttattgatgg
     4561 gaaagcttta gcggccaaat tgcaggggca gttggctgaa aagactgcaa aattaaagga
     4621 agaaacagct ctagtgcctg gtttggtagt gattttggtt ggggataatc cagccagcca
     4681 agtctacgtt cgcaacaagg agaggtcagc tcttgcggct ggttcccgta gcgaagtagt
     4741 gcgagttcca gagaccatta ctcaagagga attgttagac ctgattgcta aatacaatca
     4801 ggatccagct tggcatggga ttttggttca gttgccatta ccaaaacaca ttgatgaaga
     4861 ggcggttcta ttggctattg acccagaaaa ggatgtggat ggtttccatc ctctaaatat
     4921 gggacgcctt tggtctggac atccagtcat gattccatca acacctgcag gaattatgga
     4981 aatgtttcat gaatatggga ttgaattaga aggtaaaaat gcggttgtca tcggtcgttc
     5041 taatatcgtt ggaaaaccca tggctcagct tcttttggcc aagaatgcaa cagtgacttt
     5101 gacccactca cgcacccata atcttgctaa ggtggctgct aaagcagata ttctggtggt
     5161 cgcaatcggt cgtgccaagt ttgtgactgc tgactttgtc aaaccaggtg cggtagtcat
     5221 tgacgttggg atgaatcgtg atgaaaatgg taagctctgt ggggatgtgg attatgaggc
     5281 agttgcccca cttgctagct atattacgcc agtccctgga ggtgtcggcc ctatgaccat
     5341 tactatgctg atggagcaaa cttatcaagc agcacttcgg acattggata gaaaataaga
     5401 gaaacattcg attaatagag tgtattttct atagctatat ctaaaatggt agaaatgaat
     5461 attaaatttt agatataaga ttacaaaagg aggtctgcgt ctccttttta ttgtataata
     5521 aaagtgagag gaaaaaagga tgaaagtgat taatcaaacc ttattagaaa aagttattat
     5581 tgaacgttct cgttcaagtc ataaaggaga ttatggtcgt ctgctgctgc ttggtggtac
     5641 ttatccttat ggaggtgcca tcatcatggc tgctttagca gctgtaaaaa gcggtgcagg
     5701 attggtgacc gttggaacgg acagggaaaa tatcctggct ctgcacagcc atttacctga
     5761 gcctatggcc ttttctcttc aagaccagca attgttaaaa gagcaattgg agaaggcaga
     5821 agttgtcttg ctggggcctg gtttacgaga cgatgcttct ggagaaaatc tagtaaaaca
     5881 ggtctttgtt aatttaagcc aaaatcagat tttgattgta gacggaggtg ccttgaccat
     5941 tcttgctagg acaagtttgt catttccctc aagccagctt atcctaactc ctcaccaaaa
     6001 agaatgggaa aaattatctg gaattacgat tgaaaagcaa aaggaagatg caacggctag
     6061 tgttctgact tccttccctc aaggaacaat tttggtggag aaaggcccag ctactcgtat
     6121 ttgggaagtt ggccaatctg attattacca gttacaggtt ggtggtcctt atcaggcgac
     6181 tgggggaatg ggagatactc tggctgggat gatcgcagga tttgtaggcc aattccgaca
     6241 ggccagtctc tacgagcgtg tggcagtagc gacccatctt cattcagcca tagcccaaga
     6301 actatctcaa gaaaattatg tggtcttacc gacggaaatc agcaggtatc ttcccaaaat
     6361 aatgaaaatt atttgtcaac aagagagggc aagaaaagat aaacttgttt aaaatagaca
     6421 gataaatcta cttccttctc ttaatttatg attcttgaaa tgcgcctgct ttaaggctaa
     6481 actagacgta tcaaagttaa gagtttgttt ttgtaactgg aaag
//
LOCUS       DAVHIU010000097         6389 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVHIU010000097 DAVHIU010000000
VERSION     DAVHIU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.099"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(30..1118)
                     /locus_tag="VVN06_001649"
     CDS             complement(30..1118)
                     /locus_tag="VVN06_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU5563146.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1136..1804)
                     /locus_tag="VVN06_001650"
     CDS             complement(1136..1804)
                     /locus_tag="VVN06_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU5563147.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(1823..4261)
                     /locus_tag="VVN06_001651"
     CDS             complement(1823..4261)
                     /locus_tag="VVN06_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU5563148.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4412..5710)
                     /locus_tag="VVN06_001652"
     CDS             complement(4412..5710)
                     /locus_tag="VVN06_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5563149.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            complement(5727..6035)
                     /locus_tag="VVN06_001653"
     CDS             complement(5727..6035)
                     /locus_tag="VVN06_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5563150.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6081..>6389)
                     /locus_tag="VVN06_001654"
     CDS             complement(6081..>6389)
                     /locus_tag="VVN06_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5563151.1"
                     /translation="VPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQLADA
                     ALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKELHKK
                     "
BASE COUNT         2068 a         1340 c          954 g         2027 t
ORIGIN      
        1 accatttgga aaacagtagt ccttattgtt tattttgaat ttacataggc atctacagcg
       61 ataatagctt gagcaacatc tgaaggcgaa atcttaaacg gcatctgatg aattgtctca
      121 ccctccatag ttgcttgttt accaactttt attaaatcat catatccaac ttgatccaaa
      181 tgcatttctt ttagagttgt tggcatacca atttttttgt aaaactcaat atacttatca
      241 agttcttctt taggtctatt ttccaataat agttgtacta aagttccata agctactttt
      301 tcaccatgtg ttaaatgatg aatgtcacct gtcaatgcag taaaaccatt atgaattgca
      361 tgcgccgcag ctaatcctcc actttcaaaa cctagaccac tcaataaagt attagcttca
      421 acaatatttt ctaatgctgg tgtcaccact ttagcttcac aagctgccat agcctgtaaa
      481 ccatctgcaa acagcgtttc ttcacatttc ttcgcaattg caactccagc caatgtttgc
      541 tgttgtccca acatagtttt tccatttgcc tgcataaccg cacgcgcctc aacccaagtt
      601 gctaaaccat ctgcaatacc agacgctaat aaacgcttag gggcttgtga aataactttt
      661 gtatcaacca aaactaaatc tggattttta gaataaaata gataatgatc aaatgcacct
      721 tcatctgtat aaataacaga taaagcagat acaggtgcgt cggtcgatgc aattgttgga
      781 gcaataataa caggcttttc aatcaaatct gcaatagctt ttgcgctatc aatcgtcttt
      841 cccccaccaa gaccgataat actatcacaa ttttctttct cagccaaggc aacaactcga
      901 ttgatttcat tgtcagaagc ttcaccatta aatagcgcca gaacaatatg gaaaccatac
      961 ctatgtagat aatcttcaaa tcgttttcca acaatatcat aaaccaactg atcgcataat
     1021 agaatcgggt aatttcccaa atccaaaatt gatttggcat tttcaaacaa ggcattttcc
     1081 ccctgaatat atctagaagg actagcaaaa attctcatat gtatttcctc tctatctaaa
     1141 tggaacgact attttgagta acaaaccaat cgtcagaaaa atcatcaacc gccttttgga
     1201 tagatggcat ggcgaaagct gattcaaaaa catctgctcc tgctgtaaca gcatgcgcac
     1261 ctgcagctaa agcattattt acttgtgcta catttttaaa ggatgcagct aaaatcttac
     1321 taggagagtt ctgtctatca atagcaagag ctaattgacg aatgacagaa tttgaatcaa
     1381 tgttcagatt ttccattcta ttataatatg gagctaggta atccgctcct gcttcgatag
     1441 ctaataatcc ctgaataact gtataaatag ctgttgcagt gatatggtag ccctcttttt
     1501 ttagcacctt tattgcacgt aatccagctg gagtaacagg tactttgata aatatatcat
     1561 ctcctgcttg tcttcgaatt tcatgagcat cctttaagat gccttcaaaa tcttgagaaa
     1621 tcacctgaac atgaatagag ggtgtagagc caatcaattc tcttacatct ttgattcgtt
     1681 caaaaaaatt aatagaaccc tctctttttg caatagtggg atttgaagtt accccagcta
     1741 gcggcaaaat ttcagaccac tttttaatct catctaaatt taatgtgtca agcataaatt
     1801 ccataaaaag aacctcttta ttttacaaag tatgctcagt acgtcctata atgtcatctt
     1861 gggttgcctt agaaagaaca ttgaagaatg cagagtatcc tgcaacacga acaattaagt
     1921 ctctgtgttt ttcaggatgt ttctgagcgt caatcagcgt ctctctggaa acaacattgt
     1981 attgaatatg gtacccatgt aaacgattaa agaatgttcg taacaaagca attagtttta
     2041 atttatcttc ttctttggct aacgtttgag gatttacttt ctgatttaag agaaccccac
     2101 ctacgatttc atctgttggt aattttgaaa cagattttaa aacagatgta gggccgtgtt
     2161 gatccatatt atgtgatggt gaacaaccct ctgctaacgg tgtacccgcg ttgcgtccat
     2221 ctggagttgc taatgttcca cgtccctgcc ctacgttggc tgagatagaa gatgttcctg
     2281 aataacgaat tcctccaatt ggacctcttc cataacgtgt attaggatat ttagcaattt
     2341 catcaacata aatgtcataa gcagcagtaa ccaatttgtc agcataatca tcatcattac
     2401 catacttagg tgcatcatga atcaacattt cttgaatgac cttaccttct tctccggcat
     2461 aatctgtttc cagtgcatgc caaagctgac ttgggcttat acgttcttcc tcaaacacca
     2521 attttttaat tgcagctaat gaatccgaca aatttgcaat tccaacttgc aatcctgata
     2581 tataatcata tactgctcca ccttctttaa ggtgttttcc acgaccaata caatcatcag
     2641 tcaatgctga acatagaata tcaggaactt ctcgttccaa tgataaatca atagaatttt
     2701 caacaataac actcattcgt gtcaaatatc ttagtgtttt atcccaagca ttttctaatt
     2761 cagaaaagtt cttcatatcc ttaaaatgac caaagcttgg tgcaaaccgt ttacccgaag
     2821 ccggatcaat tccatcattc atcgtgataa gtagaacctt agggaagttc atataactca
     2881 tacctgtgca acgatagccc catttccccg gaactgccgt ttcaacacat ccaatggcac
     2941 tgtaatcata agcatcatct tccaatactc cttttgcaat aaaagaagga ataataatct
     3001 catcattatt aaatgcaggc ataccaaaac caagtttcat cacttcaata cactcattca
     3061 tgaaacgagc atctaaacct gcatggtaac gtacagttag attaggttgc ggtagatggg
     3121 tttgtgcaac tgattttaat accaaataag ataatgggtt aacagcatcc ttcttatctc
     3181 gagtctgtcc accaattgta acattttgat ataaaggact tcctgctgaa gaaaatgtat
     3241 gtgattgact gcgaacctta ttaattgtaa ttgtcttaat ccaaagattt gtcagacgtt
     3301 caacaatgct atcttctgtt tctttaccac tttctaaatc agccttcata tatggataca
     3361 tatattgatc aaaacggcca tatgaaagag agtggccatt agattcaatt tgtaaaatac
     3421 attgaataaa ccaaactgat tgaatagctt ctgcaaaagt agttgccggt tcatatggga
     3481 ctctagagca aatatctgca atctcaagta attctttctt acgtttagga tttgcatttt
     3541 cggctaaact tttagcaaga gcaacaaagc gctttgcata tactttaata gcatcgatta
     3601 cgataaatat agagtcgtaa aaatgatatt tatcaatact tgctggatct gttaaatcta
     3661 gagctacctt cgctttacga gcccgctctt caaaacctct taaaccaaat tgcaaaagtt
     3721 tctgatagtt aactgctaag tgagcatctc cagaattcat cttaccttcc ataccgaaga
     3781 atcctgtttc catataaaca gacacttctt caggtaataa ggcaccagct ctagcacgta
     3841 aattattatt ttcccaaaac ggagcaatac ttctaagttg ttcttttgtt tcttctgtaa
     3901 tatagaaaac atctccatca cgcttttcaa aaagatccaa ctcattgaga acaaattcta
     3961 gcgtatattc cggaaaaata ggagcatctt tattggaaga agcttgattt cccgcaatca
     4021 tagattcttc ttcaatatag atagacatat tttccaaaat ttctttcagc atatatgcac
     4081 gttttaggac attaggttgt tccttgtatc gttcataggc gcgtgttgct agaactgctc
     4141 tctcagcatc aatataaggt tttttattta aaacatcttc tcgatatttg ttcatccttt
     4201 cagtaaggct gccaaaatat tctgtcttga ttgttgttct tgctacttct gtatttacca
     4261 tcgtaagcac ctttctttcg aaaatgtttt tatttctttc gaaaatataa taccgatttt
     4321 tatagaaatt ttcaagcttt taactagaaa aattttaact ctctaatttt tagcacaaat
     4381 gtgtgagttt aacagtaaca cacaaatacc tctaagattg tttgatttca ttttggcaag
     4441 ctaaacgatc ttgtatctta aagaatggaa agtaaaccat tgtagacatc gccaatacta
     4501 ataactgagt cacaacacct tgccatccac ccaccaaaaa tcctgataga atagctggtg
     4561 tactccaagg caatgttacc cctgagaatg gttgcatgaa acctgttgca atagctccat
     4621 atactatcac agctgcaagt acaggaacaa gaatgaaagg tacaaacata actggattca
     4681 tgacaatcgg aaatccaaat acaactggct catttacgtt aaatattgct ggaaaagctg
     4741 caacttttcc taaggcttgg tattgttttg attttgctgc aaaaagcatg gcaactacaa
     4801 gaccaaacgt aatccctgaa cctgatagaa ttaaaaatga atctaaaaat tgttgagtaa
     4861 caatatgtgc accattttct aatgatagat taccagaggc taacatagct ttattagcat
     4921 caagattaga taaaagcaga gctgtcacta ctccatttac taccgattgc ccatgaacac
     4981 caaaccacca caaaaatgat ataaagaatg caattccaat agcaccatac aaagatccag
     5041 ttaaaccttg caacggaact tgaatagcag aataaatcat ttctatgaat gttccgccat
     5101 tagtcaatga cttcgctaaa atatatacaa tcatagaaga taagaaaatt acaaatgctg
     5161 gaatcattgc ttcaaactgt ttggcaatag cttgtggaac ttgttctggc atcttaataa
     5221 caatttttct ctttataaag aaggtataaa tacttcctac taccaaacct ataatgatag
     5281 caccgataat tccttggcct ccaaaccaaa ctttactaat agcgtcccca atcgcctcac
     5341 cttgtttagg gatataagat gatcttagca aaataaagaa tgcagataca gatagaactc
     5401 cagctggtaa agcctctact ccgctattct tagcataaga ataggcaatt gaaaaacaag
     5461 aaattagacc cataatagca aaagttcctg aatatacttg cataaacggc tctgtccaat
     5521 tagctccaaa aacactagca atgctcttat ttaatccttc gaacggcaat tgtcccataa
     5581 tcaagaacaa actgccaact actgtcaatg gcaaaattgc taacatccca tcttttagag
     5641 ctataatgcc acgcatattc acaaacttca tcatcggtgc aatgattttc tgaacatcca
     5701 tctttgacat aataaatctc cttttcttac ccactaatca aagatagggc caaatctaat
     5761 acttttttcc catctaacat accatagtcc atcatcggaa taacagctat cggaacatca
     5821 cacttatcac aaatttcttt tgatttatct aatgtataag caacttgtgg acccaatagt
     5881 gcaacatcta tatttggcgc ataatccgct aatttagact gagaaaacgc ctctatttct
     5941 gcctcaactc cactagattg cgctgcaatt ttcatattat ttacaagcat accagtagaa
     6001 aaacctgctg cacaaaacaa accaatcttc accattatgt tttcctcctc tatgttaata
     6061 acaatgataa tactctagta ttatttttta tgaagttctt ttctcaaact aataatttct
     6121 ttgattaaat taatctccgt catactagtc atgagatgat cttgtgaatg aacaaagaga
     6181 gctgtaattt ctgtctttgt accagacgct tcctgtgcca agaattttgt ttgtagatta
     6241 tgagcttcta ataaggctgc atcagctaac tggatttctt cttcacatcg ttcacatgta
     6301 ccgttcttta tgtaatctaa tgctttataa atatgttgtt tcgcatcgcc agcatataaa
     6361 attaaaccca tgataatttg atctggaac
//
LOCUS       DAVHIU010000098         6292 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVHIU010000098 DAVHIU010000000
VERSION     DAVHIU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.100"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(262..1335)
                     /locus_tag="VVN06_001655"
     CDS             complement(262..1335)
                     /locus_tag="VVN06_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU5563152.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            1491..2168
                     /locus_tag="VVN06_001656"
     CDS             1491..2168
                     /locus_tag="VVN06_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563153.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            2161..3135
                     /locus_tag="VVN06_001657"
     CDS             2161..3135
                     /locus_tag="VVN06_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU5563154.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(3184..5127)
                     /gene="thrS"
                     /locus_tag="VVN06_001658"
     CDS             complement(3184..5127)
                     /gene="thrS"
                     /locus_tag="VVN06_001658"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5563155.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5185..5361)
                     /locus_tag="VVN06_001659"
     CDS             complement(5185..5361)
                     /locus_tag="VVN06_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU5563156.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(5368..5508)
                     /locus_tag="VVN06_001660"
     CDS             complement(5368..5508)
                     /locus_tag="VVN06_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU5563157.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(5938..6138)
                     /locus_tag="VVN06_001661"
     CDS             complement(5938..6138)
                     /locus_tag="VVN06_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563158.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
BASE COUNT         1746 a         1180 c         1419 g         1947 t
ORIGIN      
        1 aaaaatggat acgagaatgt tcagttagct ttttatgtat ttgcctttgt cttacttcct
       61 tttttgcttt ttctaagtgg catcttttta atctataatg gggtcatttt gttgaagcga
      121 gagggacgtt ccaagcccaa ttatctttca atgctatttg atttttattg actattagat
      181 tctatttaaa aagacttaga atgcacatga aagtatgtat tctaggtctt ttgttttgta
      241 tacatttaat tttcaagagt attatctatt tttccatgcc tggtaacgag tatcaatcaa
      301 taactgggta aggcgaccca aggtttctct ttcttctgga gtgagggatt gagcttggac
      361 aaagagatga tgaatgtatt caagcgcctt taaggaagcc aagtcattga tagagctaat
      421 accagcctca agaatagtgc caaagaagag gtcgaagtag agctgaagtt cttcgtcagg
      481 gactgtggcc acccattctt taaaggttgt gtctacttgc tggctatcac tgttggtctt
      541 atccagttgg acgaagtgct tgtcctcaat ctgccaacta aaggtatcgt gctgggcgat
      601 gccacccagg gcagtactct gaacgatgat ttggtgagca ggaatttcca gcatcatacc
      661 gataatggaa ccttgtggaa tgaatatctt gcttctatcc attatccttt gataacccgc
      721 agtctgtgtc aattcttgat ggagaccagg tgcatcaaat gtataaactg ctgtgatctg
      781 attttgcaaa ctttgctcaa tttggctagc agcatagata gcgagatttc ctcccttgga
      841 atgcccggcc agaataacct tttgcttagg atgatgagca aaaaagttct ttaaatagcg
      901 aagggcgtgc ttttgagcag gaatttcctt catataggtc aggtggaaat cttccttcca
      961 gccaatgata ctgtcatctg tcccacgaaa gacaatcaga taggtatcga ggctgacacg
     1021 ataagtcata gccgcaaatt gcttttgcag ttcagggtcg atgtcgttga taaaatggga
     1081 gagtttgcaa tttttgaagc gcttgtgttg agccaattca tctaataatt gaaggcgatt
     1141 tttgctagta agcatggtgg gctctcttgg aacctgaggt gctagatcta aaagacgttg
     1201 aggaagtgtg gagaccagat tatcaaagga gaggtaggtg atttctatta aggttagaat
     1261 gtctaactca ttcaagggaa ggtcgtaata agaatcatat gcgacatctt tcagatagtc
     1321 aaaaatattg gccataagag ctcctttctt tttatttatc ttagcaaatt tccctcaaat
     1381 tagctagtag catagcctgt ttgtactggc taaaaacagg ctatttcaaa ttcagtttca
     1441 gaccatctag catggaaaaa tctgttataa taatagaaaa ggagaagcgc atgcacaaga
     1501 ttttattaat agaagatgat caggtcattc gtcaacagat tgggaaaatg ctctctgaat
     1561 ggggatttga agtggtcctg gtagaagact ttatggaagt tttgagtcta tttgttcagt
     1621 cggaacctca tctggtcctc atggatattg gtttgccctt gtttaatggt tatcactggt
     1681 gtcaggaaat ccgcaagatt tccaaggtac ctatcatgtt tctttcttcg agagaccagg
     1741 ctatggatat tgtcatggca atcaatatgg gggcggatga ctttgtgacc aagccttttg
     1801 accagcaggt tcttttagct aaggttcagg gcttgttgcg tcgttcctat gagtttgggc
     1861 gtgatgagag tttgctggaa tatgctggtg ttatcctcaa taccaaatcc atggatttac
     1921 attatcaagg gcaagtcttg aatttgacca agaatgaatt ccagatttta cgcgtgttat
     1981 ttgagcatgc aggcaacatc gtagcacgtg acgacctgat gcgggaactt tggaacagtg
     2041 actttttcat tgatgataat accctctctg tcaatgtggc tcgtttgcgt aaaaagttgg
     2101 aggagcaggg attggtagga tttatcgaga ccaagaaagg aatagggtac ggattgaagc
     2161 atgcttgatt ggaaacaatt ttttctagcc tatctgcgct cccgtagtcg tctttttatc
     2221 tatctgcttt ctttggcatt tcttgtctta ctctttcagt ttttatttgc cagtctagga
     2281 atttacttcc tctacttttt cttcttgtgt tgctttgtaa ccatattatt tttcacttgg
     2341 gacatattgg tggaaacgca ggtctatcgc caggaacttc tctatggaga gagggaagcc
     2401 aagtctcctt tggaaatagc tttagcagaa aaattagaag cgcgtgagat ggaactctat
     2461 cagcagaggt caaaagcaga aagaaaactg acggatttgc tggattacta taccttgtgg
     2521 gtccatcaga taaagacccc cattgcagcc agtcaactct tagttgcaga agtggtcgac
     2581 cgccaactga agcagcagct agaacaggaa attttcaaaa tcgactccta taccaaccta
     2641 gttttacagt acctgcgttt agaaagtttc catgatgatt tgctcttaaa gcaggttcaa
     2701 attgaggact tggtcaagga aataattcgt aaatatgctc ttttctttat tcaaaaaggc
     2761 ttaaatgtca atctacatga ccttgataaa gaaatcgtga cggataaaaa gtggctgcta
     2821 gtggttattg agcaaatcat ctcaaacagt ctcaagtaca ccaaggaagg tggtctggag
     2881 atttatatgg atgaccaaga gctttgtatc aaagatacgg gaatcgggat aaaaaacagt
     2941 gatgtcctcc gagtatttga acgtggcttt tcaggataca atggccgttt gacccagcag
     3001 tcctctggac ttggccttta tctatctaag aaaatttctg aagaactggg gcaccagatt
     3061 cgtatcgagt ctgaggtcgg aaaaggaacg acagtgcgga ttcagtttgc tcaagtgaac
     3121 ttagtccttg agtaaaaata gatgagaaaa agcctccaga ttggaggctt ttatgctaga
     3181 ctcttatttc tcaacgcgtg atttgttggc gatatcagct aggatagctt gaacaaaatt
     3241 atcaactgag acagtttgtg tttctttttg gccgtagcga cgaacgttga ctgtttcgtc
     3301 ttccatttct ttgtctccaa caattaattg gtaaggaatc ttgctggttt gtgaagcacg
     3361 gatcttgaac tgcatttttt cattgcgctc atctacgtct gcacggacac cgcggtcacg
     3421 gagtttcttg gccacttccc aagcgtagtc cacgtgtttt tcgttagata ctgggatgag
     3481 ggttacttgg tgtggtgcca gccatgttgg gaaggctccc ttgtagttct caatcaagat
     3541 agctgtgaag cgttccatag ttgagataac cccacggtgg atcatgactg gacggtgatc
     3601 ttcgccatca gctccgatgt atttgaggtc gaagcgttct ggcaacaaga aatcaagttg
     3661 gatagtagaa agggtttctt cttttccaag ggcagtttta atctggatat ccaattttgg
     3721 tccgtagaag gctgcttcac cttcggcttc aaagtagtcc acgcccattt catcaagagc
     3781 tgcacgaagc atggtttggg cattttccca catctcatcg ttatcaaagt acttatgagt
     3841 atcttgaggg tcacgaagag agaggcggaa gcggtagtca gtcaagttga agtcttcata
     3901 aacatcgata atcaactgaa gggcacgttg gaattcttct tggatttgtt ctggagtaac
     3961 gaataggtga ccgtcgttga gtgacatttc acgtacacgt tgaaggccag tgagggcacc
     4021 agatttttcg taacggtgca tcataccgat ttcagcgata cggattggca attcacggta
     4081 agagtgaaca tggtgtttaa aaacttggat gtggtgcgga cagttcattg gacgaaggac
     4141 aaattcttcc ccgtcaccca tgtccatggt tgggaacatg tcttcttggt aatgatccca
     4201 gtgaccagaa gtcttgtaaa gctcaacaga agcaagtggt ggagtgtaga cgtgttggta
     4261 gccagaagcc aactctttgt ttacgatgta gcgttccaat tcacgacgga tagtcgcacc
     4321 atttggcaac cagaatggca aaccttgtcc cacttcttgt gaaatcataa agaggtcaag
     4381 ctctttacca agtttacggt ggtcacgttc cttagcttct tcacgcattt gaaggtagtt
     4441 tttcaagtct ttcttgtcaa accaagctgt accgtagata cgttgcatca tagcgttgtc
     4501 gctgtttcca cgccagtacg caccagctac atggagaagg tggaagattt ggatacgacc
     4561 tgttgatgga acgtgaggtc cacggcagag gtctacatat tcaccctgac gatagatagt
     4621 caaaccgcct tcgtcttctg agtgttcttc aatcaattcc aacttgtaag ggtcattttt
     4681 gaagatttca cgtgcctcgt ctttagtcac ttcttcacga atagatggga agttttcttt
     4741 gacgattttt tgcatttctt cttcgatacg aggaaggtct tcgttagaga tttgaccagc
     4801 tgtgttgtca gtatcgtagt agaaaccatc ttcgatggct ggaccaactc ccaagtgaat
     4861 gtctgggaaa agacgacgag ctgcttgggc gaacaagtga gctgctgagt gacgcaagat
     4921 tggaagggca tcttcgtgat caggtgtcac aatttcgatg cttccatctt cagtgatagc
     4981 gcgagtagtg tcgatgagtt tgccgttgaa tttaccagcc aaggcttttt tagctaggga
     5041 attgctgata gattgggcaa tttcaaaagt tgttacgcca gattcgaatt cacgaacagc
     5101 gccatctggg aaagtaatgt taatcatgat gttctccttt tttattattt catagtttct
     5161 aagcattgat tttttatcag cagactactt ccgagtccta acgttcaatt gcttttcttt
     5221 atcagagagg aagttttcca tttccgtcag aatgtctata tccaaacgtt gagaaagttc
     5281 taatagccac cagatatttt ctgaaagttt ttgttccagt gtgtagggtg tttcatcata
     5341 gtagcgtcct tgctttgtca tcaccagtca ttggaaattt ccaatatcat tagataaagc
     5401 caagaggtct tcttctaccg tccacttgaa atcatgatgc ttaacttcca gttcgtgata
     5461 agcttgtcgg attgcccaac tgcgctccac taattcttgc aactccataa ttagtatcct
     5521 aataaaaaaa tgcgacatcc tcgaaaggac gtcgcaacgt ggttccacct tcatttatgt
     5581 acctcaaaag agagggacac ctctgatcgg ctctaacgtg gccaccgttt tatgttttca
     5641 taaaaaactc aagagtagta tcagtttaga cttcctatgc gtttccagca accacgcact
     5701 ctctagtaga aagggactaa gtgacttgtc tctatggatt attatagcat gattgtgaga
     5761 tttttcaagg attttgtgaa agtttttgtt tttcattttt tgtagaatat gcttgatgct
     5821 aataccagaa tccatgccga ccagagcaac tgtttcatag attagcaagt aaattagtat
     5881 aaactcacca aaggttatga tagtaaagta aaagagcagt ccgttaccaa ctaaccatca
     5941 aagtgagaaa cggaaacggt agctatagag taacgataca atcaagatta ccagaggtgt
     6001 aaagaagaga agattgttta tatagagttc cttgagtgta agggggtcag aaacaactag
     6061 taagaacaac tcataaagtg gccaataaaa gaataatatg accagatagt aggggagatg
     6121 ggaatagaat ctacgcatga taatcacctc gttttaaaaa acagacaact tagatctctt
     6181 gctatgatta ttatagcatg tttaaaacaa gaaagtaaat ttgttgacaa agaagatttt
     6241 tcttttcact agaatcatag ttagttttct tggtagaaat attttgataa ta
//
LOCUS       DAVHIU010000099         6214 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVHIU010000099 DAVHIU010000000
VERSION     DAVHIU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.101"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            27..1727
                     /locus_tag="VVN06_001662"
     CDS             27..1727
                     /locus_tag="VVN06_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU5563159.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1720..2196
                     /gene="ilvN"
                     /locus_tag="VVN06_001663"
     CDS             1720..2196
                     /gene="ilvN"
                     /locus_tag="VVN06_001663"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU5563160.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2262..3284
                     /gene="ilvC"
                     /locus_tag="VVN06_001664"
     CDS             2262..3284
                     /gene="ilvC"
                     /locus_tag="VVN06_001664"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5563161.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3391..3627
                     /locus_tag="VVN06_001665"
     CDS             3391..3627
                     /locus_tag="VVN06_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563162.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3865..4221
                     /locus_tag="VVN06_001666"
     CDS             3865..4221
                     /locus_tag="VVN06_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5563163.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            4271..5521
                     /gene="ilvA"
                     /locus_tag="VVN06_001667"
     CDS             4271..5521
                     /gene="ilvA"
                     /locus_tag="VVN06_001667"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU5563164.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1916 a         1039 c         1414 g         1845 t
ORIGIN      
        1 tttttaagag gaaagaaagg ggaataatgg agaaaatcag tttagaatct cctaagacgg
       61 ggtcggacct agttttggaa acacttcgtg atttaggagt tgataccatc tttggttatc
      121 ctggtggtgc ggttttgcct ttttatgatg cgatatataa ttttaaaggc attcgccaca
      181 ttctagggcg ccatgagcaa ggttgtttgc atgaagctga aggttatgcc aaatcaactg
      241 gaaagttggg tgttgccgtc gtcactagtg gaccaggagc aacaaatgcc attacaggga
      301 ttgcggatgc catgagcgat agcgttcccc ttttggtctt tacaggtcag gtggcgcgag
      361 cagggattgg gaaggatgcc tttcaggagg cagacatcgt gggaattacc atgccaatca
      421 ctaagtacaa ttaccaagtt cgtgagacag ctgatattcc gcgtatcatt acggaagctg
      481 tccatatcgc aactacaggc cgtccagggc cagttgtaat tgacctacca aaagacatat
      541 ctgctttaga aacagacttc atttattcac cagaagtgaa tttaccaagt tatcagccga
      601 ctcttgagcc gaatgatatg caaatcaaga aaatcttgaa gcaattgtcc aaggctaaaa
      661 agccagtctt gttagctggt ggtggaatta gttatgctga ggctgctacg gaactaaatg
      721 aatttgcaga acgctatcaa attccagtgg taaccagtct tttgggacaa ggaacgattg
      781 caacgagtca cccactcttt cttggaatgg gaggcatgca cgggtcattc gcagcaaata
      841 ttgctatgac ggaagcggac tttatgatta gtattggttc tcgtttcgat gaccgtttga
      901 cggggaatcc taagactttc gctaagaatg ctaaggttgc ccacattgat attgacccag
      961 ctgagattgg caagattatc agtgcagaca ttcctgtagt tggagatgct aagaaggcct
     1021 tgcaaatgtt gctagcagaa ccaacagttc acaacaacac tgaaaagtgg attgagaaag
     1081 tcactaaaga caagaatcgt gttcgttctt atgataagaa agagcgtgtg gttcaaccgc
     1141 aagcagttat tgaacgaatt ggtgaattga cgaatggaga tgccattgtg gtaacagacg
     1201 ttggtcaaca ccaaatgtgg acagctcagt attatcccta ccaaaatgaa cgtcagttag
     1261 tgacttcagg tggtttggga acaatgggct ttggaattcc agcagcaatc ggtgctaaaa
     1321 ttgctaaccc agataaggaa gtagtcttgt ttgttgggga tggtggtttc caaatgacca
     1381 accaggagtt ggctattttg aatatttaca aggtgccaat caaggtggtt atgctgaaca
     1441 atcattcact tggaatggtt cgccagtggc aggaatcctt ctatgaaggc agaacatcag
     1501 agtcggtctt tgataccctt cctgatttcc aattgatggc gcaggcttat ggtattaaaa
     1561 actataagtt tgacaatcct gagaccttgg ctcaagacct tgaagtcatc actgaggatg
     1621 ttcctatgct aattgaggta gatatttctc gtaaggaaca ggtgttacca atggtaccgg
     1681 ctggtaagag taatcatgag atgttggggg tgcagttcca tgcgtagaat gttaacagca
     1741 aaactacaaa atcgttcagg agtcctcaat cgctttacag gtgtcctatc tcgtcgtcag
     1801 gttaatattg aaagcatctc tgttggagca acagaagatc cgaatgtatc gcgtatcact
     1861 attattattg atgttgcttc tcatgatgaa gtggagcaaa tcatcaaaca gctcaatcgt
     1921 cagattgatg tgattcgcat tcgagatatt acagacaagc ctcatttgga gcgcgaggtg
     1981 attttggtta agatgtcagc gccagctgag aagagagctg agattttagc gattattcaa
     2041 cctttccgtg caacagtagt agacgtagcg ccaagctcga ttaccattca gatgacggga
     2101 aatgcagaaa agagcgaagc cctattgcga gtcattcgcc catacggtat tcgcaatatt
     2161 gctcgaacgg gtgcaactgg atttacccgc gattaaaaat ccaacttaaa tttattaaac
     2221 cagcctaaaa ggcaataaat aatagaaaag agagaaaagc tatgacagtt caaatggaat
     2281 atgaaaaaga tgttaaagta gcagcacttg acggtaaaaa aatcgccgtt atcggttatg
     2341 gttcacaagg gcatgcgcat gctcaaaact tgcgtgattc aggtcgtgac gttattatcg
     2401 gtgtacgtcc aggtaaatct tttgataaag caaaagaaga tggatttgat acttacacag
     2461 tagcagaagc tactaagttg gctgatgtta ttatgatctt ggcgccagac gaaattcaac
     2521 aagaattgta cgaagcagaa atcgctccaa acttggaagc tggaaacgca gttggatttg
     2581 cccatggttt caacatccac tttgaattta tcaaagttcc tgcggatgta gatgtcttca
     2641 tgtgtgctcc taaaggacca ggacacttgg tacgtcgtac ttacgaagaa ggatttggtg
     2701 ttccagctct ttatgcagta taccaagatg caacaggaaa tgctaaaaac attgctatgg
     2761 actggtgtaa aggtgttgga gcggctcgtg taggtcttct tgaaacaact tacaaagaag
     2821 aaactgaaga agatttgttt ggtgaacaag ctgtactttg tggtggtttg actgccctta
     2881 tcgaagcagg tttcgaagtc ttgacagaag caggttacgc tccagaattg gcttactttg
     2941 aagttcttca cgaaatgaaa ttgatcgttg acttgatcta cgaaggtgga ttcaagaaaa
     3001 tgcgtcaatc tatttcaaac actgctgaat acggtgacta tgtatcaggt ccacgtgtaa
     3061 tcactgaaca agttaaagaa aatatgaagg ctgtcttggc agacatccaa aatggtaaat
     3121 ttgcaaatga ctttgtaaat gactataaag ctggacgtcc aaaattgact gcttaccgtg
     3181 aacaagcagc taaccttgaa attgaaaaag ttggtgcaga attgcgtaaa gcaatgccat
     3241 tcgttggtaa aaacgacgat gatgcattca aaatctataa ctaattagaa atatatagcg
     3301 ctggagatga ttttatgaaa aagattatga gaaaaattgc atcgttatta ttggttctag
     3361 ttgtataatg taattacacc gtcggtaata gtgctagcag accaaaataa agcagattgg
     3421 tcgtatgatg aaaatgctgt aattaacatt tatgatgatg ctaattttga agatggtagg
     3481 ttgcatatga cctttgaaca attcttcaaa ttggcacaaa tagctagaga agaaggtctt
     3541 gaaattcatt ctccgtttga gagagctggt gcgactaaat ctgctcgtta tatagcgaaa
     3601 tggattttga gaaataaaaa atattaacaa atatagttgg taaatcatta ggacctaaat
     3661 cagctgttag attcggagaa gctttatcct atattgaagg tcctcttcgc agaataaatg
     3721 agacgataga tggcggttta tatcaaatag agcaaattat tgcatctgga ttgaaagaat
     3781 cgggtttaaa tgactggact gcgaaaactt tagcttcagc tattcgtggg atattagatg
     3841 tacttattta ggggttgaaa tcatatgaat attaccaatt tgttttctat caagacagga
     3901 tgtgatgaaa ctgataggca actgcaaaaa ctattttttc agttggattt acaattggga
     3961 gaattgacag atcaactaag aaaattagat tctaattttg ttcctcgtag tcaatttgta
     4021 gacacgttgg atttgaatga tgtagaatat aaagaaattt taaactattt tatcttccat
     4081 cgtaatgata gtgaagaaag tttggtagaa tggttatatg attggatttc cacaaatcgt
     4141 tatgaacttc ctaaagagtt ttcgattcgt atggctcata aataccatga aagtgttact
     4201 gaaattttcg gagatgaata actaaaaaac agtcattagt gactgttttt tatagaaaaa
     4261 gaggttttat atgttaagtt caaaagatat aatcaaggct cacaaggtct tgaacggtgt
     4321 ggttgtgaat actccactgg attacgatca ttatttatcg gagaagtatg gtgctaagat
     4381 ttatttgaaa aaagaaaatg cccagcgtgt tcgctccttt aaaattcgtg gtgcctatta
     4441 tgccatttcc cagctcagca aggaagaacg tgaacgtggg gtagtttgcg cttctgcggg
     4501 aaatcatgcg cagggagtag cctatacttg taatgaaatg aaaattcctg ctactatctt
     4561 tatgcccatt actacgccac aacaaaagat tggtcaggtt cgcttttttg gtggggattt
     4621 tgtaactatt aaactagttg gagatacctt tgatgcctca gccaaagcag ctcaagaatt
     4681 tacagtctct gaaaatcgta cctttattga tccttttgat gatgctcatg ttcaagcagg
     4741 tcaaggaaca gttgcttatg agattttaga agaagctcga aaagaatcga ttgattttga
     4801 tgctgtcttg gttcctgttg gtggtggcgg tctcattgcc ggggtttcta cctatatcaa
     4861 ggaaacaagt ccagagattg aggttatcgg agtagaggcg aatggagcgc gttccatgaa
     4921 agctgccttt gaggctggag gtccagtcaa actcaaggaa attgataaat ttgcggatgg
     4981 gattgctgtg caaaaggtag gtcagttgac ctatgaagca actcgtcaac atattcaaac
     5041 tttggtaggt gtcgatgagg gattgatttc tgaaaccttg attgaccttt actctaagca
     5101 agggatagtc gcagaacctg ctggagcagc tagtatcgcc tctttagagg ttttagctga
     5161 atatattaag gggaaaacca tttgttgtat catttctgga ggaaataatg atatcaaccg
     5221 tatgccagaa atggaagagc gtgccttgat ttatgatggt atcaaacatt actttgtggt
     5281 caatttccca caacgtccag gagctttgcg tgagtttgta aatgatatcc tggggccaaa
     5341 tgatgatatc acacgttttg agtatatcaa acgagctagc aagggaacag gcccagtatt
     5401 aattgggatc gctttagcag ataagcatga ttatgcaggt ttgattcgta gaatggaagg
     5461 ttttgatcca gcttatatta acttaaatgg taatgaaacg ctttataata tgcttgtctg
     5521 aggactaata aaaaaatatc atattatttg tacaactgat gtatatgtgc tataatgaag
     5581 atgaagaaag gggacgattc ctatggtttt agaaaactta tcgtgcaatt cttacttctg
     5641 ttcattatag cacttatgat gtttggctca gatttccatc cttcattttg atttcctatc
     5701 tattgacaag catagtcaca ctgtctttaa tactcttcga aaatctcttc aaaccacgtt
     5761 agctctatct gcaacctcaa aacagtgttt tgagcaactt gcggctagct tcctagtttg
     5821 ctctttgatt ttcattgagt ataaggtatg atttgatttc tttttgttga caaatatacc
     5881 atattaaaaa gatatataag taattaactg agcttatctg tcttgtcatc tttattaagg
     5941 atggtttaga taatcgggtg tctgcttcta ggctagcacc tcaatatcca aaggagtgat
     6001 gaatttgaag gacataagga atacctatct ctcagatgat ttattgagga agaaagatag
     6061 gagtttttga gctagtgaag gcttggattt ctaaaggtta gaactatcat cttcagttct
     6121 taaatcgaag aaataagcta tcttacggaa atagagaagc attttttaag aacttgaata
     6181 atttcgcacc ttaagagggt aataatacag tatt
//
LOCUS       DAVHIU010000100         6135 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVHIU010000100 DAVHIU010000000
VERSION     DAVHIU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.102"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..809
                     /locus_tag="VVN06_001668"
     CDS             <1..809
                     /locus_tag="VVN06_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU5563165.1"
                     /translation="SRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVD
                     AVEIDYLYQYVVSSRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVD
                     LASHISPLLRRLDNRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEI
                     GFLVLYFARFQEKRARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDE
                     LINLYPDLDFIVTTVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            802..1230
                     /locus_tag="VVN06_001669"
     CDS             802..1230
                     /locus_tag="VVN06_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563166.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            1208..1657
                     /locus_tag="VVN06_001670"
     CDS             1208..1657
                     /locus_tag="VVN06_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563167.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            1685..1996
                     /locus_tag="VVN06_001671"
     CDS             1685..1996
                     /locus_tag="VVN06_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU5563168.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            2022..3107
                     /locus_tag="VVN06_001672"
     CDS             2022..3107
                     /locus_tag="VVN06_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU5563169.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            3118..3813
                     /locus_tag="VVN06_001673"
     CDS             3118..3813
                     /locus_tag="VVN06_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5563170.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            3850..5820
                     /gene="tkt"
                     /locus_tag="VVN06_001674"
     CDS             3850..5820
                     /gene="tkt"
                     /locus_tag="VVN06_001674"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5563171.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKFP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
BASE COUNT         1787 a         1052 c         1384 g         1912 t
ORIGIN      
        1 tcagtaggaa tcgtcgtagt actagtattc atgttgtagc accttcaaaa cctactattg
       61 ttgatgagaa aatttacagt gtctgtcaaa aaattattca agaaattgaa caatatttta
      121 agatgaaggt tgatgcagtt gagattgact atctttatca atacgttgta tcttcgagat
      181 tgcaaaaacc attttcttcc gggaagcttc ctttttctca gcgagtttta gatgtcactc
      241 attactattt tagccgtatg tgtatggaca atagagagat tgaaacgaca gatcctgact
      301 ttgttgactt ggcgagtcat atcagtccct tactgaggag attagataat agagtacaga
      361 ttaagaatag tcttttatca caaattcttt taacctatcc taatctggtt aaagagttaa
      421 caactatttc taaagaagtg agtctagtat ttggttttgc gtccttgagt ctggacgaga
      481 ttggttttct agtcttatat tttgcacggt ttcaagaaaa gcgagcacgt cctctaaaaa
      541 cagtagtgat gtgtacatca ggtgtcggaa cttcagagct tttacgagca cgattagaaa
      601 agcaatttac tgaattggat attattgatg tagttgctta tcatcaatta gatgagctga
      661 taaatctata tccagattta gatttcattg tgacgacggt agctttgcag gaaccagcaa
      721 gtgtcccgtt tgtcctagtt agtgtttttc taaccgaggg tgataaacaa cgtcttcaag
      781 caaaaattca ggagataaac tatgaataat ctttcgcttg tccttatgga tatatctgtt
      841 caaaatcgtc aagaagccta caaagaatta gcaaatcaaa tcagccttct tgtttctgaa
      901 gatacagaaa aaatagaaga gcttctatat taccgtgaga gacagggaag tatagaggtt
      961 gctaaaggtg ttcttctacc acattgtgaa ggaaactttc aacatcatgt cttagtgatt
     1021 actagattaa aatcacctat cagagaatgg tcgaaggata tccagtgtgt tgaccttatt
     1081 atcggtttgg ccattgcagt atcacaggac aagtcatgta ttaaaacatt gatgagaaga
     1141 ctagcagatg aatcattcat aaatcaatta aaacagttaa caaaagaaga attacgggag
     1201 ataatatatg gaaatcaaag atattcttaa tgtgagtctg atccagacgg atttacagat
     1261 gcagagcaaa gaagaggttt ttgaggcatt agctcaacta ttggttgaga cgggttatgt
     1321 gtctgataga gaccaattta tcgaaggtct ttatcagaga gaggcagaag gacagaccgg
     1381 tattgggaat tatattgcta ttccccatag caagagttct gctgtggaga aggcgggggt
     1441 agtcatagct ataaatcaca atgagattcc ttgggagacc attgatggga aaggggtcaa
     1501 agtaattgta ctctttgcag ttggtgatga tacagaagct gctagggagc atttgaagac
     1561 cttatcactc tttgctcgaa aacttggtaa tgacgaagtt gttgccaaat tagttcgggc
     1621 tcagacatct gatgatgtga ttgcagcttt ttgttaataa gaaaaaattt tggagggtat
     1681 ccgtatgaaa attgttggtg ttgcagcttg tactgtggga attgcccaca cttatattgc
     1741 acaggaaaaa ttagagaatg ccgcaaaggt agctggacat gtgattcatg ttgagactca
     1801 ggggacaata ggggtagaaa atgaattgag tcaagagcag attgatgcag cggatgtagt
     1861 tattttagca gttgatgtta agatttctgg tatggaacgc tttgagggta aaaagattat
     1921 caaggttcca acagaagtgg cagtcaaatc tcccaataaa ctgattgcta aagctgttga
     1981 gattgttacg aaataactga aaatatttaa ggagaaaata tatgttgaaa cacttaaact
     2041 taaaaggtca cttattgaca gccatttcct atatgattcc aattgtttgt ggtgcaggat
     2101 tcttagttgc cattggttta gcaatggggg gtggtgttcc tgacgctctt gtagcaggaa
     2161 aattcactat ctgggatgct ttagcaacta tgggtggtaa agcccttggt ctcttgccag
     2221 ttgttattgc tacaggtttg tcttactcga ttgctggtaa accagggatt gcaccaggtt
     2281 ttgttgttgg tctaattgcc aattctgttg gttcagggtt tatcggtggt atcttgggag
     2341 gttatatagc tggtttcttg gttcaagcga ttattaaaaa ggtcaaagta ccaaactgga
     2401 ttaaaggttt aatgccaacc ttgattattc cttttgtagc ctctttggta agtagtttga
     2461 ttatgattta tattattgga gcgcctatcg cagcctttac caactggttg acgagcttat
     2521 tacaaagctt gggaagtgct tcaaatggtt tgatgggggc agttattgga gttctcagtg
     2581 ctgttgactt tggtggccca cttaataaaa cagtctatgc gtttgtgttg actttacagg
     2641 ctgaaggtgt gaaagaacca ttgactgctt tgcaattggt gaatactgct acaccagttg
     2701 gatttggatt ggcctatttt atcgcgaaat tactcaaaaa aaatatctat actcaagagg
     2761 aaatcgaaac attgaaatcg gctgttccta tggggattgt caatattgtt gaaggtgtaa
     2821 ttccgattgt tatgaataac ttggttccag gtctcattgc aacaggtatc ggtggtgctg
     2881 ttggtggtgc tgtttctttg acaatgggtg ctgattctgc cgtgccattt ggtggagtgc
     2941 ttatgttacc aaccatgact cgtccagtag ctggtatttg tgctttgtta gccaacattg
     3001 tagtcacagg acttgtctac gcgattttga aaaaaccaat aaaacataca gaaccagtta
     3061 tgactgttga agaagagatt gatttgtcag atattgaaat tttgtaagag ggtaacgatg
     3121 tcaagaattg aattttcacc atctttgatg accatggatt tggacaaatt caaagagcag
     3181 attacttttt tgaatgataa agtagcatct tatcatatcg atattatgga tggccatttt
     3241 gttcccaata ttaccttgtc tccttggttc attcaagaag ttcaaaaaat tagtgacaca
     3301 cctttatcag ttcatctgat ggtcacagac ccaacctttt gggtagatca agttctcgat
     3361 ttacaatgtg agtatatttg tattcatgct gaagttctga atggtcttgc ttttcgtttg
     3421 attgataaaa ttcatgatgc aggtctaaag gctggtgttg tccttaatcc tgaaacacct
     3481 gtttctacaa tctttcccta cattgattta cttgacaaag taactattat gactgtagat
     3541 ccaggttttg caggacaacg ctttttggag tctaccttgt ataaaatcca agaactccgt
     3601 cagcttagag ttcagaatgg ttatcactac atcattgaga tggatggttc ttcgagtcgt
     3661 aagactttca aacaaattga tgtggcagga ccagatattt atgttatagg tcgtagtgga
     3721 ttatttggtt tggatgacga tattgccaaa gcctgggata tctgttctag agattacgaa
     3781 gaaatgaccg gaaaaacaat gccaatcaaa taatggtttg agaagaaatt tattagttag
     3841 gaggaatata tgtcactaca atcagttaac gccattcgtt ttcttggcgt agatgctatt
     3901 aacaaatcta attctggtca cccgggaatc gtcatgggtg ctgcgccaat ggcttatagc
     3961 ctatttacaa agcaccttag aattacacct gagcagccaa actggattaa ccgagatcgc
     4021 tttatcttgt ctgcgggtca tggatcaatg ctactgtatg ctctcttgca tttaacaggg
     4081 tataacgatg tatccatgga cgagattaaa aatttccggc aatggggatc taagacacct
     4141 ggtcatcctg aagtgacgca tacgtctggt gtggatgcga catctggtcc gcttggtcag
     4201 ggaatttcta ctgccgttgg tttcgcccaa gcagagcgtt ttttagctgc taagtacaac
     4261 aaagatggtt tccctatttt tgaccattat acttatgtta ttgctggaga cggtgacttc
     4321 atggaaggag tgtctgcgga ggcggcttct tatgcaggtc atcaagcttt agataagctt
     4381 atcgtcctct acgactccaa cgacatctgc ttggatggtg agaccaaaga tactttctct
     4441 gaaaatgttc gcgcccgtta cgatgcttat ggttggcata cagttctggt agaagatgga
     4501 acagatttag cagcaatttc tacagcaatt gagacggcca agttttctgg taagccgagt
     4561 ttgattgaag tgaaaacggt aattggttac ggctcaccca ataaaagtgg tacaaatgct
     4621 gttcatggtg caccactagg agcagaagaa acaggagcaa ctcgtaagtt tttgggatgg
     4681 gattacgatc catttgaagt accagaggaa gtatattctg atttcaagac aaatgtagcg
     4741 gatcgtggtc aggaggcata cgatgcttgg gctagtttgg tgtctgatta caaggttgct
     4801 tatcccgaag ttgctagtga gattgacgct attgtagctg gaaaattccc tgtaaccatt
     4861 actgaaaaag acttccctgt ctatgagaat ggcttctctc aagcaactcg taattcgtcc
     4921 caagatgcta ttaatacagc agcagcagtt ttaccaacct tcttaggtgg atcggcagac
     4981 ttagctcact ccaacatgac ctacatcaag gcagatggct tacaagataa atataatcca
     5041 ttaaaccgca atattcagtt tggggtacgt gaatttgcca tgggaacaat cctcaatgga
     5101 atggctcttc atggtggttt acgagtttat ggcggaacct tctttgtttt ctctgactac
     5161 gtcaaagctg ctattcggct atcagccatt caggagttgc ctgtaactta tgtctttacc
     5221 catgattcaa ttgccgttgg tgaagatggt ccaactcatg aaccagttga acatttggca
     5281 ggtttacgct caatgccaaa cttgactgtt atccgtccag cggatgcccg tgaaactcaa
     5341 gcggcttggc atcatgcctt gaccagtacc accactccaa ctgtcattgt cttaacccgt
     5401 caaaacttgg tagttgaaga agggacagac tttggtaagg tcgctaaagg agcctacgtc
     5461 gtgtatgata ccccgggatt tgatactatt atcattgcta caggatctga ggtcaatcta
     5521 gctatcaaag ctgctaagga attggtttta caaggtggta aagtacgtgt ggtatctatg
     5581 ccctcaaccg aactatttga tgctcaagat gctacctaca aggaagacat tttaccatct
     5641 aagactcgtc gtcgtgtggc cattgaaatg gcagcgaccc aaagttggta caagtatgtt
     5701 ggtttggatg gcgcggtcat cggtattgac atcttcggtg cgtctgcccc agctcagact
     5761 gtgattgata attatggatt tacggtagag aatatcgttg ctcaagttaa gtccctatag
     5821 aaaccaatta caatgaagat acagctgttg tcagactagc agatgtagtg atagacacta
     5881 atcagatgat tggttattta aaaactataa tgagaatgta ataatttatc tacgaaagtt
     5941 atagtagata gtatacagaa tagagtatac tctgaaacgg ttgcgaagta cgctaatcac
     6001 tttgctactg atctagatag tttctttaat caataaacac agcatccaca gattgactta
     6061 ggatattgta agttttttga aagctagaga gaaggtctct aaaattaaaa aacgcatagt
     6121 ataggatgtt gaaat
//
LOCUS       DAVHIU010000101         6093 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVHIU010000101 DAVHIU010000000
VERSION     DAVHIU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.103"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..475
                     /locus_tag="VVN06_001675"
     CDS             <1..475
                     /locus_tag="VVN06_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5563172.1"
                     /translation="AISSLENILLKVSSNTLIYLKALNTLSNFYSLVGREQEYEANYS
                     HLMELYQTKNFEHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETIELCKQRQTSY
                     QLAPLLILVGNAGAKFLDKEQVKNYYIEARELCKIYNNPLMLMKIENYLKELDTV"
     gene            780..1400
                     /locus_tag="VVN06_001676"
     CDS             780..1400
                     /locus_tag="VVN06_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563173.1"
                     /translation="MKSMRILFLLVLIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLLYSILLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYMRDLLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIATLIFF"
     gene            1485..1928
                     /gene="tsaE"
                     /locus_tag="VVN06_001677"
     CDS             1485..1928
                     /gene="tsaE"
                     /locus_tag="VVN06_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5563174.1"
                     /translation="MYTKNEEELQSLGERLGHLLEKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFLFGGGVTVIEWG
                     NLLGDALPDTYLELEILKEEDGRRLHFQAKGLRAEKLLEELQHGV"
     gene            1918..2436
                     /locus_tag="VVN06_001678"
     CDS             1918..2436
                     /locus_tag="VVN06_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563175.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNDKIAGLVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGRQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2444..3460
                     /locus_tag="VVN06_001679"
     CDS             2444..3460
                     /locus_tag="VVN06_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5563176.1"
                     /translation="MVKKIIGMVLAFLAVTVLGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTDQWAGNSDSMILLTVNPHTKKTMMLSLERDIL
                     TKIQHKDGSIEEAKLNAAYAGGGAELAIETIQRMMNIHIDRYIMVNMQGLQQLVDAVG
                     GITVNNTLGFPISISDQEEFNTISIGVGEQTINGEEALVYSRMRYQDPEGDYGRQKRQ
                     REVIQKVVEKVLSLNSVSHYQSILKALSTNMQTNIDLSAKSIPSLLGYKDSFKTIETQ
                     QLKGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3545..4801
                     /locus_tag="VVN06_001680"
     CDS             3545..4801
                     /locus_tag="VVN06_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5563177.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGNKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4856..6022
                     /gene="recA"
                     /locus_tag="VVN06_001681"
     CDS             4856..6022
                     /gene="recA"
                     /locus_tag="VVN06_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5563178.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
BASE COUNT         1906 a          997 c         1405 g         1785 t
ORIGIN      
        1 ggctatttct tcattggaaa atatattatt aaaagtctct tcaaatactc tgatttattt
       61 aaaggcgttg aatactctat ctaatttcta ttccttagtg ggtcgtgaac aagaatatga
      121 ggcaaactac tctcatttga tggagttata tcagacaaaa aattttgagc atcaagagtt
      181 tttatttggc tacatcagag ttcgttacaa ctacgctcac tacctagtgt caaaggaaaa
      241 atataacgaa gccatccaag aagctcttga gacgattgaa ctctgtaaac aaagacagac
      301 aagctaccaa ctggctcccc tacttattct tgtaggaaat gctggagcca aatttctaga
      361 caaagaacaa gtcaaaaatt attatataga agcaagagag ttatgtaaga tttataacaa
      421 tcctttaatg ttgatgaaga tagaaaatta tttgaaggaa ttagatactg tttagttaat
      481 cttgacatta tagtttttct gaaacgttaa ataacctgta aggctgatag tgaaatgaat
      541 actatcagcc ttatttcaat ttatattctt ccaaaatctc ttcaaaccac atcagctcta
      601 tctgcaacct caaaacagtg ttttgagcaa cctgtggcta gcttcctagt ttgctctttg
      661 attttcattg agtattagtc tctcatgttt gtttagcgtc taaaagagac atttgagaag
      721 attggaaaca gttctctctt ttctccgagt ataatgattg gtaggaaggg agagaaaaga
      781 tgaaatcaat gagaatctta tttttgttag ttttaattca aatcagtttg agtagctgtt
      841 tcctatggaa ggaatgcatc ttgtccttta aacaaagtac agcttttttc atcggaagta
      901 tggtctttgt ttcaggaatc tgtgctggag taaattatct ttatacccgt aagcaagaag
      961 ttcatagtgt cctagccagt aagaagtcgg tgaagcttct ttacagtatc ctgctcttaa
     1021 ttaatttgtt aggagctgtc cttgttttgt cagacaactt gttcatcaaa aatacgttgc
     1081 agcaagaatt agttgacttt ttattgccat cctttttctt tctatttggg ctagatttgc
     1141 tgattttttt acccttgaaa aaatacatgc gtgatttgct tgctatgcta gacagaaaaa
     1201 agacagtttt ggtgactatt ttagcaacac ttctcttctt aagaaatcca atgaccattg
     1261 tctcacttct gatttatatt ggactgggtt tgttttttgc agcctatctt gtcccgaatt
     1321 ctgttaaaaa ggaagtttcc ttttatggtc atattttccg agatcttgta ttggtcattg
     1381 ctacgctcat tttcttttag agaaagcttg tttttatgaa tgttatgatg gtaagtttag
     1441 caggaaaata gacacaaaag aaaagttttt ggtataataa gagtatgtac acaaaaaatg
     1501 aagaagagct acaatcctta ggggagcgtt tgggtcatct attagaaaag aatgatgttt
     1561 taatcttaac tggagaactg ggcgcaggta aaacgacctt tactaaagga cttgcaaagg
     1621 gattacagat ttcccagatg attaagagtc caacctacac catcgtaaga gagtatgaag
     1681 gcagactgcc gctttatcat ctagatgttt atcgaattga aggagatgcg gattctatcg
     1741 acttggatga gtttctcttt ggtggtggag tgactgttat tgagtggggg aatctcttag
     1801 gagatgcctt gccagatact tatttagagt tggaaattct aaaagaagaa gatggtcgca
     1861 gattacattt ccaagcaaag ggtttgcgtg cagaaaaact cttagaggag cttcaacatg
     1921 gagtatgaat tgctcattag ggaagcagag cctaaagatg cagctgaatt agtggccttt
     1981 ttaaatcgtg taagtttgga gacagatttt accagcttag acggagatgg tattctcttg
     2041 accagtgagg agatggaaat attcctcaac aagcaggcta gttcggacaa tcagataacc
     2101 ttacttgcat tcttaaatga taaaattgct ggccttgtaa atattacagc tgaccagcgc
     2161 aagagagtcc gtcatattgg agatctcttc attgtgattg gaaaaagata ttggaataat
     2221 ggattgggaa gtttgttgct agaggaagcg atagagtggg cacaagcaag tggcattctg
     2281 cgtcgtctcc aactgactgt ccaaactcgt aatcaagctg cagttcatct ttatcaaaag
     2341 catggctttg tcattgaagg tcgccaagag cgtggagcgt atatagaaga agggaaattt
     2401 attgatgttt acttgatggg taaactgata gattagtaga aatatggtta aaaaaattat
     2461 tggaatggtg ctagcttttc tagctgtgac agttttgggt gtcggtgttt ttgcttacac
     2521 tatttatcag caaggtacag agactttggc aaaaacctat aaaaaaatag gggaagaaac
     2581 caaggttatt gaagcaacag agcctttgac cattctgttg atgggggttg atactgggaa
     2641 cgtagagcgt acagaccaat gggctggaaa tagtgattcc atgattctct tgactgtcaa
     2701 tccacatact aagaagacca tgatgttgag tttggagcgt gatatcctga ctaaaattca
     2761 acataaagat ggtagtattg aagaagcgaa gctaaacgct gcctatgctg gtggcggtgc
     2821 agaacttgct atagaaacaa ttcaaaggat gatgaatatc catatcgatc gctatattat
     2881 ggtcaatatg caaggattgc aacaattggt agatgcagtg ggtgggatta cggtcaataa
     2941 tacactaggt ttcccgattt ctatcagtga ccaagaagaa tttaatacca tttctatcgg
     3001 tgttggagag cagacaataa atggagaaga agcgcttgtt tattcacgca tgcgttatca
     3061 agacccagaa ggagactacg gacgtcaaaa gcgtcagcgt gaagtcattc aaaaagttgt
     3121 tgaaaaagtt cttagtttga atagtgtcag tcactatcag tctatcttaa aagcgctaag
     3181 tactaatatg cagactaata ttgatttatc tgcgaaaagt attccaagct tgttgggcta
     3241 taaagattca tttaaaacta ttgaaacaca acagttgaag ggtgaaggag agatacttca
     3301 aggtgtttct taccagattg tttcgagagc acatatgttg gaaatgcaaa atatactccg
     3361 acgttctttg ggacaagaag aagttactca gcttgaaacc aatgcggttt tatttgaaga
     3421 tttatttggc agagcacctg ttggtgatga agataattaa cttttgatac aaataaaaaa
     3481 atcaatcgta ggaaaattcc tgcgattttt tcaaaaaaat acgaatagat aggtaggagg
     3541 aaacatgaaa gcagaaatca ttgctgttgg aacagagatt ttgacaggac agattgtcaa
     3601 caccaatgcc cagtttttgt cggaaaaact agctgagatt ggggtagatg tatattttca
     3661 gacggctgta ggagacaatg aagttcgtct cttgtctttg cttgagattg ccagtcaacg
     3721 tagcagtctg gtgattttga caggcggttt ggggccaact gaggacgacc taaccaaaca
     3781 aaccctagct aaatttttag ggaaagcatt agtctttgat cctcaggctc aggagaagtt
     3841 ggatatcttt tttgccctgc gaccagacta tgcccgaaca ccgaataacg aaagacaagc
     3901 tcaaattgta gaaggagcga ttccactgcc aaacgaaaca ggactggctg ttggaggaaa
     3961 attagaagta gacggagtga cctatgtcgt ccttccaggt ccgccaagtg aattgaaacc
     4021 catggtctta aaccaacttc tacccaagtt gatgacaggg aacaagctgt attcccgagt
     4081 tcttcgtttc tttgggattg gcgagagcca gttggttacg attttggctg atttaattga
     4141 taatcagatc gatcctacct tggcccctta tgccaagaca ggagaagtca ctctacgtct
     4201 gtcaacaaag gctagcagtc aagaagaggc gaatcaagcg ctggatatct tggaaaatca
     4261 aatcttggac tgccagactt tcgaaggaat ttctttacga gacttttgct atggttatgg
     4321 ggaagaaact agtttagcca gcattgtggt agaagaactg aaaaggcaag ggaaaaccat
     4381 cgcggctgca gagagtttga cggcaggtct tttccaagct accgtggcga atttttctgg
     4441 agtttcaagt atatttgagg gtggttttgt gacctatagc ttggaggaaa aatcaaggat
     4501 gttggatatt cctgccaaga atttggaaga acatggtgtg gtgtctgaat ttacagctca
     4561 gaagatggct gagcaggcac gaagcaagac ccagtctgat tttggaatta gtttgactgg
     4621 agtggcagga ccagatagcc tagaaggaca cccagttggg acagtcttca taggcttggc
     4681 gcaagatcaa ggaactgagg ttatcaaggt taatattgga ggcagaagcc gagcagatgt
     4741 acgtcacatt gcggttatgc atgcctttaa cctagttcgc aaggctttat taagtgacta
     4801 acttttgata taatagtaga taggtctgag gatcattaga atgtaggaga atagaatggc
     4861 gaaaaaacca aaaaaattag aagaaatttc aaaaaaattt ggggcagaac gtgaaaaggc
     4921 cttgaatgac gctcttaaat tgattgagaa agactttggt aaaggatcaa tcatgcgttt
     4981 gggtgaacgt gcggagcaaa aggtgcaagt gatgagctca ggttctttag ctcttgacat
     5041 tgcccttggc tcaggtggtt atcctaaggg acgtatcatc gaaatctatg gcccagagtc
     5101 atctggtaag acaacggttg cccttcatgc agttgcacaa gcgcaaaaag aaggtgggat
     5161 tgctgccttt atcgatgcgg aacatgccct tgatccagct tatgctgcgg cccttggtgt
     5221 caatattgac gaattgctct tgtctcaacc agactcagga gagcaaggtc ttgagattgc
     5281 gggaaaattg attgactcag gtgcagttga tcttgtcgta gtcgactcag ttgctgccct
     5341 tgttcctcgt gcggaaattg atggagatat cggagatagc catgttggtt tgcaggctcg
     5401 tatgatgagc caggccatgc gtaaacttgg cgcctctatc aataaaacca aaacaattgc
     5461 catttttatc aaccaattgc gtgaaaaagt tggagtgatg tttggaaatc cagaaacaac
     5521 accgggcgga cgtgctttga aattctatgc ttcagtccgc ttggatgttc gtggtaatac
     5581 acaaattaag ggaactggtg atcaaaaaga aaccaatgtc ggtaaagaaa ctaagattaa
     5641 ggttgtaaaa aataaggtag ctccaccgtt taaggaagcc gtagttgaaa ttatgtacgg
     5701 agaaggaatt tctaagactg gtgagctttt gaagattgca agcgatttgg atattatcaa
     5761 aaaagcaggg gcttggtatt cttacaaaga tgaaaaaatt gggcaaggtt ctgagaatgc
     5821 taagaaatac ttggcagagc acccagaaat ctttgatgaa attgataagc aagtccgttc
     5881 taaatttggc ttgattgatg gagaagaagt ttcagaacaa gatactgaaa acaaaaaaga
     5941 tgagccaaag aaagaagaag cagtgaatga agaagttccg cttgacttag gcgatgaact
     6001 tgaaatcgaa attgaagaat aagctgttaa agcagtggag aaatccgcta ctttttcgat
     6061 ttttgattca agtttttaga ttatatatag tag
//
LOCUS       DAVHIU010000102         6027 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVHIU010000102 DAVHIU010000000
VERSION     DAVHIU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.104"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            81..1484
                     /locus_tag="VVN06_001682"
     CDS             81..1484
                     /locus_tag="VVN06_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU5563179.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            1599..2237
                     /locus_tag="VVN06_001683"
     CDS             1599..2237
                     /locus_tag="VVN06_001683"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU5563180.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            2249..2689
                     /locus_tag="VVN06_001684"
     CDS             2249..2689
                     /locus_tag="VVN06_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU5563181.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            2682..3113
                     /locus_tag="VVN06_001685"
     CDS             2682..3113
                     /locus_tag="VVN06_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563182.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            3104..3574
                     /locus_tag="VVN06_001686"
     CDS             3104..3574
                     /locus_tag="VVN06_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5563183.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            3599..4372
                     /locus_tag="VVN06_001687"
     CDS             3599..4372
                     /locus_tag="VVN06_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU5563184.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            4369..5169
                     /locus_tag="VVN06_001688"
     CDS             4369..5169
                     /locus_tag="VVN06_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5563185.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            5228..>6027
                     /locus_tag="VVN06_001689"
     CDS             5228..>6027
                     /locus_tag="VVN06_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU5563186.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFDPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTNYWGNIDISSLQGEFSSIDYFTEKDEHVK"
BASE COUNT         1993 a          790 c         1306 g         1938 t
ORIGIN      
        1 ttcaagtaag cgttcacata ttgaaaaaat aaaacgtggg gattataata aacttaatca
       61 aggacgaaga gagaagaaaa atggaagcgg ttttagcaat agatttaggt gcgacttctg
      121 gaagagcaat cgttggttac ctttctgaaa ataaactggt aatggaagaa ataaatcgct
      181 tttctaatct acctattaga gtaaaagggc atttatcttg ggatattgac tttctactag
      241 ctaaaattct tgaaagtatc cgcttggcta atactagtta caagatttta tctatcggta
      301 ttgacacatg gggagttgat tttggactga ttgataatga aggtaagctg ttattacaac
      361 ctgttcatta tcgtgatgaa agaacaaagg gagtgttaaa ggaaatatct gaaatgactg
      421 aattagaaaa actgtattca gagacaggaa atcagattat ggagataaat accttgtttc
      481 aactctttaa ggcacgtcaa gaatctcctg actctttcta taagaccaat aagattcttt
      541 taatgccaga tttgtttaat tatctcttga caggtaagtt tgctacagaa aaaagcattg
      601 cttcaacaac tcaattattt gatcctagga gtcaaaattg gaatcagaat atcttaaaac
      661 tatttgaatt ggattcatct ttacttcctg aaattgtttc agagggaaat gttcttggaa
      721 ggataaaaaa ggagtatggt ttaggcgata ttcctgttgt gaatgtttgt agtcatgata
      781 cagcaagtgc gattgtctca gtacctaaga cagaaggtag tttatttatt tcatcaggta
      841 cttggtcttt ggttggagtg gaacttactt caccgattct tactaccgaa tccttcagtt
      901 atggatttac aaatgaagtc ggtaaagatg gagtgattac atttctgaag aattgtacag
      961 ggttgtggat catagaggaa ctaagacgtt catttgaacg aagagggaaa gcctattctt
     1021 ttgatgatat taggacaatg gtggagaaag aaaaagaaaa tcttcctctg attgatactg
     1081 aatcaactga atttgcaaca gaatctgata tgcacaagac tttgacagaa tatctagctt
     1141 atcatcatga aactagagag tggacagatg gacaactatt taagattgtt tatgaaagcc
     1201 tagctgaaac gtataggaaa gcgatagagt tactagaaga actaactcat aaggtttata
     1261 agaggatata tgtgattgga ggaggtgcta gagccagtta ctttaaccaa atgattgctg
     1321 atagaactgg taaagaggtt cttacaggtt cgactgaggg tacagctgtg gggaatattg
     1381 ttgtgcagct catagctatg ggacaattaa aagggatgga agaagctcac catgttattg
     1441 aggagttcct acaattagag agttattact cccaaaagaa ttaaaaagat tgagagtttg
     1501 taaatttgcc tccctccccc ttcttaagtt ttgtacaaga aggggggata attggtgaat
     1561 tgaaaaatat ttagtgtttt gatatgagga ggacaagaat gtcagatgta aaacaagaat
     1621 taattaagta tggcaagaag ctagtagaaa cagatttgac gaaaggaaca ggtgggaatc
     1681 tcagtgtttt cgatcgtgaa aaacaattga tggcaattac cccgtcgggt attgatttct
     1741 ttgaaatcaa agaatccgat attgtagtga tggatattaa tggaaatgtt gtagagggag
     1801 aacgcttgcc atctagcgaa tggtatatgc atttgattca ataccaaact cgtgatgata
     1861 tcgatgcaat tatccatgct catacaactt atgcaacagt attagcttgt ctcagagaac
     1921 cacttccggc tagtcattat atgattgcag tggcagggaa agatgttcgg gtagctgagt
     1981 atgcaacata tggtacgaaa gaattggctg tgaatgcagc taaagcaatg gaaggtcgta
     2041 gagcagtttt actagcgaat catggaattt tagcaggtgc acaaaattta ttgaatgcat
     2101 ttaatattgt tgaagaagtt gaatattgtg caaaaattta ttgtttagct aagaattttg
     2161 gagagccagt agttcttcct gatgaggaga tggatttaat ggcagaaaaa tttaaaacat
     2221 acggtcagag aaaataggga ggatattaat gttaaaacat ataccgaaaa atatttctcc
     2281 agatttattg aagactttaa tggaaatggg acatggagat gaaatagtat tagctgacgc
     2341 gaattatcct tctgcctcat gtgcaaataa gctaattcgt tgtgatggtg taaatattcc
     2401 agaattatta gattccattc tgtatttaat gccattagat agttacgtcg atagttcaat
     2461 tcagtttatg aacgttgttt cgggtgatga tattcctaag atatggggta cctatagaca
     2521 gatgattgaa ggtcatggta cagatcttaa aacgattact tatcttagaa gagaagactt
     2581 ttatgaacgt agtaagaaag cttatgctat tgttgctaca ggagaaactt cactttatgc
     2641 taatattatc cttaagaaag gagtagttgt tgaaagagaa aatgtttaat agaggaattt
     2701 tagttgccag tcatggtaat tttgctagcg gagctctcat gaccgcagaa atgtttgttg
     2761 gtgagacaac aaatgataga gttaggacat taggtttgat gcctggagag aatattgtag
     2821 agtttgaaca ttattttaaa aatcaagtaa atgaactgtt agactcaaat caagaggtta
     2881 tcgttttgac tgacttgatt ggaggaagtc ctaataatgt ggctttgtca cggtttttaa
     2941 atttggattc agttgatatt gtaacagggt ttaatatccc tctcctagtg gaattaatat
     3001 caagttatga ttcaaaaatc aatttagaag aaattgttca aaatgctcaa aatagtttgt
     3061 ttaatgttaa acaacaactt aacgtagagg aggaagagga tttatgtcta tagagtttgt
     3121 tcgtattgat gaccgtctgg tacatggtca agttgtcact acgtggctaa aaaagtatga
     3181 tattgagcaa gttatcattg ttaatgatcg catctcagaa gataaaacac gacaatctat
     3241 tttaaagatt tctgcaccgg taggtttaaa aattgttttc tttagtgtaa aacggtttgt
     3301 ggaagtttta aactctgtgc caataaaaaa gagaacaatg ctgatatata caaatccaaa
     3361 agatgtgtat gattctattg aaggaaattt aaaattggag tacctcaatg taggacagat
     3421 gagtaaaacg gaggaaaatg aaaaggtaac gggaggtgta gctctaggtg aagaagacaa
     3481 atattatttt aagaaaatag ttgataaggg aacgagagtt gaaattcaaa tggttcctaa
     3541 tgataaagtt acaatgttgg aaaaattttt ataaaaataa tttaaggagg tacagtatat
     3601 gctattcaca caagcattac tggtgacatt agttgggatt attgccacta ttgactataa
     3661 tggaccgtta tttatgattc accgtccgtt agttacaagt gcaatggttg gcttagtctt
     3721 aggagatttc acccaaggtg ttcttattgg ttcagctctt gaattaactt ggctcggtgt
     3781 aacaggtatt ggaggttaca ctccaccaga tactatttca ggtgcgatta ttggtactgc
     3841 atttggtatt ttgtctggtc aaggagaaac tgctggtatc gctatagcag ttccaattgc
     3901 agttgctacc caacagttgg atgttcttgc aaaaacttta gatgtttatt ttgtgaaaaa
     3961 agctgataat gatgctaaaa acggagatta ttcaaagatc ggtttttatc attattcaag
     4021 tttggtttta atcactttat ttaaaattgt accaattttc ctagctatta tgcttggagg
     4081 ggaatatgtg gcagacttgt ttgctaaggt tccaccaatc gttatgcagg gacttaactc
     4141 tgcaggtgct ttactacctt caattggttt tggtatgctt ttaaatatga tgctcaagaa
     4201 aaatatgtgg gtattcttgt tgattggatt catttgttct gtgtatggag gaatgtcaac
     4261 cattgggatc tcactagttg gtattgcggt agcatacttc tacgatatga ttggaagtaa
     4321 accacaagaa acaacttcaa gtagtgatgt tgaggaggat cttgatctat gatgaataat
     4381 aaagtaacta aagttgaact taaaaaagtt ttcaaacgaa gttttatgta tggttcttca
     4441 tggaactatg agagaatgca gaacctaggt tttctatata caattcttcc agtattgaaa
     4501 aaactatacc cagacaaaga ttcagcttct cctgcaatga aacgtcacct tgagtttttc
     4561 aatactcatc aaacagcggc accatttatt cttggagtta cttccgctat ggaagaacaa
     4621 gaaggaaatg aaggtgcagc ttcaattact ggtattaaag ttggcttgat ggggccgctg
     4681 gctggtctag gagatagttt gttctggctg acactagttc ctatctgttt tagtattggt
     4741 gcgtcttatt ctaaagacgg cggtgcttta ggtatcttta tcgccttaat attgtttaat
     4801 attattaata ttcctgttaa atatttcggt ttgaaatatg ggtatactaa gggttctagt
     4861 cttatccaag aaaataatac aaaaggaaca ttgaatcgcg ttacgagtat ggcgacagca
     4921 ttagggctag tactagtggg tggtttgatt ccatcaatgg ttggtattaa ttttggatta
     4981 gaatttaaac agggggaact tgttatttct gttcaagaaa tgattacaaa attaattcca
     5041 ggatttatcc ctatggcttt gactttatta atgtgtaaat taattagaaa aggaaagaat
     5101 ccggttgtac taatctttag tgttatggct attggagtta tcctagttgt tttaggaatt
     5161 ttgaagtagt agaaagtgtg gaggtggtat ttgggatatc acctccattt tggaagagag
     5221 gtaaagagtg aaattatggt ataagaaagc tgcctcaaat tggaatgaag ccttgccgat
     5281 tgggaacggt catttaggtg gtatgattta tggttcagct acaaaagaat gtattcaact
     5341 aaacgatgag actatttggt atagaggaaa gtcagataga aataatccag actcactatt
     5401 gcatcttaaa aaaattcggg aatatctttt agatggagaa attcagaaag ccgaagaatt
     5461 gataaagtta acaatgtttg ctaccccaag agatcaaagc cactatgaat tacttgggga
     5521 actttacatt gagcatatag atattcagtc ttgtgctctt tcattgtatg aaagagagct
     5581 agatttagat acagctattt ctaatgttgt gtttgatcct aatagttgta atttacaaat
     5641 aaaaagagaa tattttacga gttttaataa gaatatttta tgttgccgta tagtgtcatc
     5701 agttcaaaac acattaaatt taaacattaa tttgggtaga aataaacggt ttaatgacga
     5761 agtatctaaa ctggattcaa gtacaatttt aatgtcggcc tctgctggag gtagaaaagg
     5821 tgttcagttt aaagtagtat gtcattctaa agttacggat ggtgaagtaa gtgtattggg
     5881 agagacaata gttattcgga atgctacaga ggtatttctt tatctcaaat caatgacgaa
     5941 ttattgggga aatatagata tttcttctct tcagggagaa tttagtagta ttgattactt
     6001 tacagaaaaa gatgaacatg taaaaaa
//
LOCUS       DAVHIU010000103         5926 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVHIU010000103 DAVHIU010000000
VERSION     DAVHIU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.105"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1096)
                     /locus_tag="VVN06_001690"
     CDS             complement(<1..1096)
                     /locus_tag="VVN06_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5563187.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKK"
     gene            1154..2320
                     /locus_tag="VVN06_001691"
     CDS             1154..2320
                     /locus_tag="VVN06_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU5563188.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2459..2785
                     /locus_tag="VVN06_001692"
     CDS             2459..2785
                     /locus_tag="VVN06_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5563189.1"
                     /translation="MYFPTSSALIEFLILAVLEKGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2772..3365
                     /locus_tag="VVN06_001693"
     CDS             2772..3365
                     /locus_tag="VVN06_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU5563190.1"
                     /translation="MTRTEYLTQLELYLKKLPEADQIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHVALLALLAAPIGIPLGIAILV
                     SLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGDQHA"
     gene            3358..4269
                     /locus_tag="VVN06_001694"
     CDS             3358..4269
                     /locus_tag="VVN06_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEU5563191.1"
                     /translation="MRKLTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLAMSKEPVY
                     DSRMEELTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSESPFISSGIDGILHIVSSSSNRFNDVIIGLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNGYILRIEGSRIKNSKLTTPNIVNIFDTDLTDSQLESTEHHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDVSSNYGSIFQFTREEPESRGTELSNPYK
                     TEKTDVKDQLIARSDDDIELISTPSRR"
     gene            4332..5396
                     /locus_tag="VVN06_001695"
     CDS             4332..5396
                     /locus_tag="VVN06_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563192.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEERIADLDTPIEK
                     NTQVEEEVSQAEVELESQQEEKMETPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEALK
                     SPTSKLETSSTHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIATINSHFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIYQEKDWTLEKILQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFSALLCVISIGIILLASLHIITRPSQASEADSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGNYLRILAFL"
     gene            complement(5633..5830)
                     /locus_tag="VVN06_001696"
     CDS             complement(5633..5830)
                     /locus_tag="VVN06_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEU5563193.1"
                     /translation="MRSLSQEKDIYSFDEPTGNLDRNSTELFLNEVEKLVNEEKIVIV
                     VTHDKDVIARASKVINMDEFH"
BASE COUNT         1778 a         1335 c         1004 g         1809 t
ORIGIN      
        1 ccttcttcca tttaaatcct ctaattaaac ctacaatcgt cccaaagcca taagcaaagt
       61 gaatggaaaa taaaagaaag ggcatcacaa ttagaaatcc atttttatgt tttaataaag
      121 tcagcaaagt gagtagtgac aaaagtagaa aataggcacc taatagtaaa gttatgaata
      181 cgaatgtgat cggtaacaat gctagactaa acacaagact caaaacaaat aaacaaggaa
      241 catagtgaaa taatgataaa cacttaaact gaacatgact tgtcaagcca atccacaaac
      301 catttaaata cttttgatgc agcattttct tgaatgttgg tcgaatatac tgataagata
      361 gaatacttgg gctatagcgg attttataac catattctcg aattctataa tgaatatcat
      421 tatcttcagt tcggccaagt tgctcattta ctaaaccaac cttctggaaa acctctcgtt
      481 tatacattcc atgaaaaata gaagaaacat atctatcctc agaactattt cgataattgg
      541 caatgctact gccaaacata ttttcctcaa caagatgcaa ggtctctgcc cattttcctt
      601 ttccttcgac aatcgtcggt ctaggccccc cacagacaaa ttcaccttgt tgaataatag
      661 ccacattgtt cattacaaaa gtctcagtaa cttttgaatg agcatcaatt tttaaaataa
      721 ggtcccctac agaatgttta actcccaggt taaaaccact agcttgattt ttcttaggat
      781 tgttatacaa tctaattgag ttaaactctg tatcttcctt tataaattgc tgaatgatag
      841 ctgtggtccc atctgtggac atagcattta taaatagaat ttcaatattc tctttaggat
      901 aggtttgatt ttttaagtct tcaattagac caggcagata tttttcttca ttgtatgcac
      961 tgattacaat ggatacgaga agactttcaa cattttgagt cactactact ctcctgaatt
     1021 tttccttcca ccattctatc ataattttca agactttccc atttttatct tgaaagccag
     1081 aaaccttact tgacattata gtgaaaaagc cttaaaataa gctgttatta aaatcagcta
     1141 gaagaggtat tatatgaaaa agcaatcact cttttttgtt ccaggtatta tcctgattgg
     1201 tgtttccttg cgaactcctt ttactgtttt acccattatt ttgggaaata tttcgcaagg
     1261 tctggaggta gaagttagtt cgcttggtgt cttgaccagc ctgcctctcc ttatgtttac
     1321 cctcttttca ccattttcta cccaactggc tcagaaaatc ggcttggagc atctcttcac
     1381 ctacagcctc ttcttcttga ccatcggctc acttattcga ctaatcaatc tgcccctgct
     1441 ctatctagga accttgatgg ttggggcaag tgtcgcagtc atcaatgtcc tgcttcctag
     1501 tcttatccaa gccaatcaac caaagaaaat tggttttctg accaccttat atgtaacgtc
     1561 tatggggatt gcaacggctc tggcttccta tctagctgtg cccattacac aagccagttc
     1621 ttggaaagga cttatcctcc tcctcacgtt actctgtcta gcaacttttt tggtctggct
     1681 cccaaatcac cgctataatc atagactagc tccacaaacc aaacaaaaaa gtcaaataaa
     1741 ggtcatgcgt aataaacagg tttgggcaat tattatcttt tcaggttttc aatccttgat
     1801 cttttacacc gtcatgacct ggttacctac catgtctatc catgcaggtc tatccagtca
     1861 cgaagctggc ttgctgactt ctatcttatc tctgattagc attccttttt caatgaccat
     1921 cccaagcctg acaaccagtt tatctactcg caaccgtcag ctcatgctca ctctggtttc
     1981 actagctggt gtggtcggca tttccatgct ctttttccca atcaataatt tcatttactg
     2041 gcttgccatc catctcctca tcggaaccgc aaccagcgcc ctcttccctt atctcatggt
     2101 caacttttca ctcaagacaa gcgcccctga aaagacagcc caattgtccg gcctatctca
     2161 aacaggaggc tatatcctag cagcctttgg gccaaccctc tttggttaca gttttgacct
     2221 gttccactct tgggtaccat ctgtagctgc cctcttgctc atcgatatcc tgatgactgt
     2281 ggccctcttt acagtggaca gagcggataa gatcctttaa acttctagtt ttagctagaa
     2341 gttttttata tataaaaatt ttacacattt ctcttgacag ggctttcttt tagatgtaca
     2401 atgtatgtgt agaaaaatta tatataaaaa tcctacacat tagaaaagga ggtttcccat
     2461 gtactttcca acatcctctg ccttgattga atttctcatc ttggccgtac tggaaaaagg
     2521 ggattcttat ggttatgaga ttagccaaac cattaagctg atcgctaata tcaaagaatc
     2581 cacactctat cccattctca aaaaattgga aggcaatagc tttctgacaa cctattctag
     2641 agagttccaa ggtcgcatgc gcaaatacta ctccttgaca aatggtggta tagaacagct
     2701 cttgacccta aaagatgaat gggcactcta tacagacacc atcaatggca tcatagaagg
     2761 gagtatccgc catgacaaga actgaatacc tgactcagct agaactctat ctcaaaaaac
     2821 tgcctgaagc tgaccaaatt gaagccatgg actatttcag ggaactcttt gatgatgctg
     2881 gagtcgaagg agaggaagaa ctcatcgcta gcttgggaac tcccaaagaa gcagcccacg
     2941 aagttctctc taatcttctt gataaaaaaa tcaatgaagc acctgctcaa aaaaataacc
     3001 gacaaatttt acatgtcgcc ttattagccc tccttgcagc acccatcggt attcctctgg
     3061 gaatcgccat cctcgtgtcc ctgttcgcaa tccttgtggc agccttgact gtcatcttgg
     3121 ctttctttgc ggtttccata ctgggtatca tcggtggatt cctattttta gttgaaagtt
     3181 tcactgtcct agcccaagcc aaatcagcct ttatcttgat tttcggttct ggtttactgg
     3241 ctatcggtgc ttcttcacta gtcttactag gtatttccta tgtagcccgc ttcttcggtc
     3301 tactcattgt tcgcttggtg caatttgttc ttaaaaaagg aaagagaggt gaccagcatg
     3361 cgtaaattga caaaaggatt tctcattttt ggtgtcgtga ctaccgttat cggctttatc
     3421 ctgctctttg taggtatcca atctgacggg attaagagcc tacttgccat gtccaaggag
     3481 cctgtctatg atagtcggat ggaagagtta acctttggca aggaagtcga aaacttagag
     3541 atcactcttc accagcacgc gctgaccatc acagactctt tcgatgatca aatccacatt
     3601 tcttaccatc catcactttc tgctcaccat gatcttatca ccaatcagaa cgatagaact
     3661 ctgagtctca ctgataagaa actgtctgaa agtccattta tctcttctgg aattgatggg
     3721 attctccata ttgtaagtag ttcatctaat cgttttaatg atgtcatcat cggactaccg
     3781 aaagggagaa ctctaaaagg gatcaacatc tcagccaatc gcggacaaac caccatcata
     3841 aatgctagcc ttgaaaatgc gaccctcaat acaaacggct atatcctccg aattgaagga
     3901 agtcgtatca aaaacagtaa actcacaacg cccaatatcg ttaatatctt tgatacagat
     3961 cttacagata gtcagctaga gtcaacagaa catcacttcc acgctgaaaa tatccaagtc
     4021 catggtaagg ttgaactgac tgccaaagat tatctccgaa tcatcctaga ccagaaagag
     4081 agccaacgaa ttaattggga tgtctcaagt aactatgggt ctatcttcca attcacaaga
     4141 gaagagcctg aatcaagagg tacggaatta agcaaccctt acaaaactga aaaaaccgat
     4201 gtcaaggatc aactcattgc gagatctgat gatgatattg agctaatatc cacaccaagc
     4261 agacgttgac aaaaacgctt acatctgcta ctatggaaac atatttatct aactaaaaag
     4321 gaggttctac catgaaacaa gaatggtttg aaagtaatga ttttgtaaaa acaacaagca
     4381 agaacaagcc tgaagagcaa gctcaagagg ttgcagacaa ggctgaagaa aggatagccg
     4441 atctcgatac accaattgaa aaaaatactc aagtagagga ggaagtctct caggctgaag
     4501 tcgaactgga aagccagcaa gaagagaaaa tggaaactcc tgaagacagt gaagcgagaa
     4561 cagaaataga agaaaagaag gcatctaatt ctactgaaga agagccagac ctttctaaag
     4621 aaacagaaaa agtcactata gctgaagaga gccaagaagc tcttcctcag caaaaagcaa
     4681 ccacgaaaga gccccttctt atcagtaaat ctttagaaag tccttatatc cccgaccaag
     4741 ctccaaaatc tagggataaa tggaaagagc aagtgcttga tttttggtct tggctagtgg
     4801 aggctctcaa atctcctaca agcaagcttg aaacaagtag cacacacagc tacacagcct
     4861 ttctcttgct cattctgttt tctgcatctt cctttttctt tagtatctac cacatcaaac
     4921 atgcttacta tggacatata gcaactataa acagccactt ccctgagcaa ctagcacctt
     4981 taactctctt ttctatcatc tctatcctag tagcgacaac actcttcttc ttttcattcc
     5041 tcttgggtag tttcgtcgtg agacgattta tctaccaaga aaaggactgg acgctggaaa
     5101 agattctcca acaatatagt caactcttgg caattccaat cttcctcact gccattgcta
     5161 gtttctttgc tttctttgac agcctacgat tttcagccct tttatgtgtg attagcattg
     5221 gaatcattct gcttgctagt ctccatatca ttaccagacc aagtcaagca agtgaagccg
     5281 actccttcta tcagttattc ttgtctgtcc ttgtgaacgg agttattatc ctcctcttct
     5341 ttgtagctga agtggcactg attggaaatt atcttcgtat cttggccttt ctttaaactt
     5401 caatcctgtc atccttggca ggattttttc tatgtcaaaa aagcaagtcg attgacctgc
     5461 ttctgcttta cttttcttgt gctaatgttt taaaatcttg tcctaggatg ggttttattc
     5521 ctagagttca aagtatgata gcgttgctat aaagttcgtt attccaaata ttatgcaaac
     5581 tagttatatg taaaattaag attagaaacc accaatctcc atttattaac ttttaatgaa
     5641 attcgtccat attaataact ttacttgctc tagcaataac atccttgtca tgagtcacga
     5701 caataacaat cttttcctcg ttcactaatt tctcaacctc gtttagaaat aattcagttg
     5761 agtttctgtc caaatttcct gtaggctcat cgaatgaata gatatccttt tcttgcgata
     5821 aactcctcat gaatagaatt ttttgtttct gaccttacga aatattggtg gctttcccat
     5881 caatctgatg actcagagct atattgagat tcaaactatc aagatt
//
LOCUS       DAVHIU010000104         5892 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVHIU010000104 DAVHIU010000000
VERSION     DAVHIU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.106"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(43..1383)
                     /locus_tag="VVN06_001697"
     CDS             complement(43..1383)
                     /locus_tag="VVN06_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5563194.1"
                     /translation="MYIFWIILYTLITNGLEIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMIGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLKDSSKKLQDNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(1396..2133)
                     /locus_tag="VVN06_001698"
     CDS             complement(1396..2133)
                     /locus_tag="VVN06_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563195.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(2138..2476)
                     /locus_tag="VVN06_001699"
     CDS             complement(2138..2476)
                     /locus_tag="VVN06_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563196.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVRYARRRYRE
                     IRQKWLKEGE"
     gene            2752..3081
                     /locus_tag="VVN06_001700"
     CDS             2752..3081
                     /locus_tag="VVN06_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU5563197.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3249..4298)
                     /locus_tag="VVN06_001701"
     CDS             complement(3249..4298)
                     /locus_tag="VVN06_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563198.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLVMGYGLPVFLVYVLLLGVGKYFLFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4295..5026)
                     /locus_tag="VVN06_001702"
     CDS             complement(4295..5026)
                     /locus_tag="VVN06_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563199.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5094..5504
                     /locus_tag="VVN06_001703"
     CDS             5094..5504
                     /locus_tag="VVN06_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5563200.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5514..5801
                     /locus_tag="VVN06_001704"
     CDS             5514..5801
                     /locus_tag="VVN06_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563201.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         1943 a         1298 c         1080 g         1571 t
ORIGIN      
        1 aataagaccg agtaacaaga actctcggtc ttattttttt catcattctg taggtatcat
       61 agtaagcacc tgacgaaaga cttgatcttg acaggtagta tttaaactgg cattgggata
      121 actttccaca atcttcataa cggtatagag accgacacct ctctcctccc ctttagaact
      181 aactccaaag gagaagattt cagaaatatc tatgccctct tctttgatgg aattttcgat
      241 gatgaaggtc tcctgtgctc cactttttaa aaaggcaatt gaaacatgag gttgaccggc
      301 ctctgcactc gcttcaatag cattatcaca aaggatagac acaatggtta gaaaatcaag
      361 cagtctcatc ccctcaacct gaatctcttc aggaacttcg acattaaaga caatgtcctt
      421 ttctctagct tttataaatt ttccagctag gagacttttg agggcacgat cccgaacatt
      481 caccaatcgg ccaaggtcat atttattgtc ctgcaatttt ttactagaat cctttaagac
      541 caagtcatag acctctttta tctgctccat atcctcctct tcaataccca gacgtaaact
      601 tgtcaagagg ttggtgtagt catggcgaaa actccttact tccttgtaaa gttcctctat
      661 atgccgacta tagcgttcca tatctctata gcgcaagacc tgctcttggt ccagtctatc
      721 atggagtttg tccttcaaat aggtatccaa tttcttgata acccccataa aaaataggag
      781 gtaaaacact aggatgagat ggcgaacagt cgttgattga ataccttgct catattcaaa
      841 gtaagacaga ctttgtatca ccaaatagta agcccccatt atccagttaa tcttagtcag
      901 ggacttttga aaacctgtat ctagaatctc ccttctcaaa ctagtaaaat catagtccaa
      961 ccatttcaaa aaggctagag aaatgaagaa attgaaaatt attatacata acccagtaaa
     1021 tgagtagcca tcatatactt gctcttgtcc caaaaatgga agcacaaaat aggagactcc
     1081 tctataaaag agattcacca atatcattgg aaagagacca taaaagaaaa ggagtttttt
     1141 aggaagccct cttaacaata agaaagacaa gcctatgcca tacaagggtt ccataaaata
     1201 agataggtaa acatttccta tcatatagcc aatcattaca aaaacaaagg ccaacagtat
     1261 cttcaaaaga aaggccttaa aaatcctctc aaaagtgaga ccaattccat ctaccttaaa
     1321 gaaaatgaca atttctagcc cattagtaat aagtgtatac aatataatcc aaaaaatata
     1381 cataaatttc ctagttcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     1441 acgcgcgatc agacagcttc caccattggg aaagaaaagg agtttttctt tcttatccaa
     1501 atgcaccaca tttgcaggat tgatgagaaa agagcgatgg cactgcaaga gacggggttc
     1561 ctgcttaaaa acctcctcta aactcgccgt aaattctagc ctgtccgtct tggtatagag
     1621 aataacacga tggggtcttg gggatgtttc gagatagtaa acctctttga aaggatattg
     1681 gaattgggca aattttgatt taaagtaaaa gcaatcttcc gccagacttt tactatcttg
     1741 actattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     1801 cttatcaatg tagtccaaag ctgacacttg gtagcgaaag gacagaggca taaactccga
     1861 gtgagtcgtc acaaagacga ttagggcata agggtcttgt tcccgaatct ttctagccac
     1921 ttccagtccc ttcatctctt catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     1981 ccccttctca tgcacctctg ccagcagttg gtccggcttg ccaaatacct caaaagagct
     2041 aggaatgata tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg
     2101 ggaaaaatca tcttctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     2161 tttgacgtat ttctctatag cgacgtctgg catacctgac agcatattga ccttcttctc
     2221 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     2281 aatttcgatg acatctcatc aaagtttccc cacattggtt ctcaagatta cttaaatttt
     2341 gaagcatatt aaaactagct ttttctgtaa caatctgtac ggtatgggct ttagttggat
     2401 gtgtttggat atagtagata tcgtcgatgt ttactttata gactgttttc tgtgtctgaa
     2461 taatcatatg tttcattgga ttctcctcct aagaaaagaa taacagaaaa gatctagccc
     2521 aagcacaccc aatcctaaac agtctttgaa aactcctaaa aggattattt tctacaacat
     2581 catgatgagc ttacaactat tttatcataa aattcactca gcgcaattca agacatttca
     2641 atgacaatta aagatttgaa taaaaaaaaa gaattaaaag tgataaacta acagtatcaa
     2701 aacacagttg ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat
     2761 acagacccca tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca
     2821 tgcaaacagg caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt
     2881 tttctagcca agactgaaac agtggataat cgattcttga ttgaactaga aaaattttac
     2941 cagacttcca gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc
     3001 gcttggcggg cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat
     3061 gagaatcaaa gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaaggcga
     3121 cgctgacgtg gtttgaagag attttcgaag agtataagtc tctacggacc aaccattatc
     3181 ctgtagtaac aaaaaagaag ctgagacaaa ctgaactcaa cttctttttt agtgtcgtat
     3241 cagcaatgtt agtcctgcat ctgacgtttt acctgataag gcaaatacaa gactagtaaa
     3301 accaaaccag ctcctagtaa ggctagaagg gctagttttt cttggaaggt aatcaaccca
     3361 acaactaatt ccacaagtaa aacaaaactg atcaatcctc gaactacctc ctgtaagcct
     3421 ttttcctttt gccccttgtc cagcggaaag agttgggtca aatactggta gtcaaaggca
     3481 tgatagaggg ccagcaactg gaaaagcaag aggtagttaa agagaactac cactgctgtc
     3541 gcaatccaag attgctcgat aaagacctgc gccagcaaag aaagcaagag cagacggaga
     3601 ctaagggcaa aaaggtctcc atttcgcaaa taagaacgca gatagagatt ttgccaaatc
     3661 ttcccagaca ccttctgaac aacctttaga ataaagtcca gataggcacg acgcttgacg
     3721 ctgtttgaaa ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa gacttgctta
     3781 cgcttgcttt cttgggaaat gacatagtcc caatccagtc cagtttcagt gaaaaatttg
     3841 ctggcctttt gacgaaagag gaaatatttt cctaccccca ataaaagcac atagaccaga
     3901 aaaacaggca agccataacc catgactaaa aacaagggcg caaataacag caagaagagg
     3961 gtctgaacaa aaatccaaaa gactagggaa atgccggttt gacgcttgag atggagcttg
     4021 atttcctctt ctccaactaa gagaaagagc ttgtctggag cctccatata ggtggcaatt
     4081 cctccccaaa gtaaaagtaa aacagacgta attcctacaa acaaaaggat aggccaatga
     4141 ttttcaggaa aatgttgtaa gagttgactg tactggtagg ctagaaagcc caacaggaca
     4201 agcaggaaca agacaaagtg gtcattgagc acatagcgca gataacaaag acactcctta
     4261 cgaaaggcct gctttctctt taaaaacaag tctttcatag atcctcctct ttggtcagag
     4321 ccaagtaaat atcattcaaa ctagcctcag gcatatcaaa ggcttcacgt agttgcagga
     4381 gattgccttt ggcacgcacc tctcccttgt gaagaatgac aaaggcatca cacatctttt
     4441 ccgccgaatc cagcacgtgg gtactcatga gaatagactt gcccttttgc ttctccactt
     4501 ccaaaagctg aatcaaatca gaaatagcca gcggatcaag accaaggaaa ggctcatcca
     4561 cgatgaaaag acttggatcc accacaaaag cacagataat catgaccttc tgcttcatcc
     4621 cttttgaaaa atgaacaggg aaccagtcta atttctgttc caaacggaac atttttaaca
     4681 agggctctac tcgttcgaaa gccacttttt gctcaatacc gtaagccata gcaaccgttt
     4741 cgatatgctc tctgagggtc aattcctcat acagactagg cgtctcagga atgtagccaa
     4801 tctgcttgcg gtagctagtc gcatctcctt gcagagtcag gccattgata ttgatggagc
     4861 cactataagg tgccaacaga ccgataatct cattgatcgt cgttgatttc ccagcaccat
     4921 tgagaccaat caaaccgacc aactgcccac tttcaacagt aaaggacaca tctttcaaaa
     4981 caggaacatg aacatagcca cctgtcaggt ttttaatttc taacatattt tctccaaatc
     5041 tggtataatg tagctatatt atatcaaaat tcagtacagt agaggtagat tttatgtcag
     5101 attgcatttt ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta tatgaagatg
     5161 agcaggtcct tgcctttctt gatatctctc aagtaacact aggacacacc ttggtcgtgc
     5221 caaaagaaca ctatcgcaat cttttggaga tggatgctac gagcgccagc caactctttg
     5281 cccaagtacc aaaagtagct caaaaagtca tgaaagtcac taaggctgct ggtatgaata
     5341 tcatttccaa ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac gttcaccttg
     5401 tgcctcgcta cagtgctgac gatgacctca agattgattt tatcgcccac gaaccagact
     5461 ttgacaaact tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat
     5521 tatccaacct actgctattt gcaggagctg cagccggaag ttatctggtt acaaaaaatc
     5581 gccaaaccat cacagatgaa gtcttgaata ccactgaccg cgttcaagct atcaaggacg
     5641 atgtggatat tatccaaaac agcctgcaaa tcattaacca gcaaaaagaa cttatcaagg
     5701 aataccaaga agacttgact tacaagttta aggtcttgga aaaggatatc cagactagac
     5761 tagctgtgat aaaagaaatg cagggaactg aagataagta aaaagagccc gacggctctt
     5821 ttttaaaacg taaacaagtt atagtgaatt gaatttagaa tagcacatca tagtttctaa
     5881 agcattgtta ga
//
LOCUS       DAVHIU010000105         5848 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVHIU010000105 DAVHIU010000000
VERSION     DAVHIU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.107"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            126..1472
                     /gene="gor"
                     /locus_tag="VVN06_001705"
     CDS             126..1472
                     /gene="gor"
                     /locus_tag="VVN06_001705"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU5563202.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            1669..2205
                     /locus_tag="VVN06_001706"
     CDS             1669..2205
                     /locus_tag="VVN06_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU5563203.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            2256..2342
                     /locus_tag="VVN06_001707"
                     /pseudo
     CDS             2256..2342
                     /locus_tag="VVN06_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2411..2626)
                     /locus_tag="VVN06_001708"
     CDS             complement(2411..2626)
                     /locus_tag="VVN06_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU5563204.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            2724..3044
                     /locus_tag="VVN06_001709"
     CDS             2724..3044
                     /locus_tag="VVN06_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563205.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(3074..3412)
                     /locus_tag="VVN06_001710"
     CDS             complement(3074..3412)
                     /locus_tag="VVN06_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563206.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(3424..4143)
                     /gene="trmD"
                     /locus_tag="VVN06_001711"
     CDS             complement(3424..4143)
                     /gene="trmD"
                     /locus_tag="VVN06_001711"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5563207.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(4133..4651)
                     /gene="rimM"
                     /locus_tag="VVN06_001712"
     CDS             complement(4133..4651)
                     /gene="rimM"
                     /locus_tag="VVN06_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5563208.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(4682..5470)
                     /locus_tag="VVN06_001713"
     CDS             complement(4682..5470)
                     /locus_tag="VVN06_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5563209.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(5574..5817)
                     /locus_tag="VVN06_001714"
                     /pseudo
     CDS             complement(5574..5817)
                     /locus_tag="VVN06_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
BASE COUNT         1672 a         1251 c         1075 g         1850 t
ORIGIN      
        1 tatttggcaa gcttttttgc aattttttgt gcgtagcaga tttttgcaat caaatcaaaa
       61 aaagaataaa atataactat caaaaaagac actctactat ttaaaagatt acaaaggagt
      121 tttcaatgag agaatatgat atcattgcta tcggtggagg tagcggagga atcgctacca
      181 tgaaccgtgc tggtgaacat ggagccaaag cagccgttat tgaggaaaag aaattaggtg
      241 gaacctgtgt caacgtcggt tgtgttccta aaaaaatcat gtggtacggg gcgcaaatcg
      301 ctgagacttt ccatcaattt ggagaagact acggctttaa gactactgat cttaactttg
      361 actttgcaac cctacgtcgc aatcgtgaag cctacattga tcgcgctcgt tcttcttatg
      421 atggtagttt taaacgcaac ggtgtagact tgattgaagg tcatgctgaa tttgtagatt
      481 ctcatactgt aagcgtaaat ggtgaactga ttcgtgctaa acatatcgtg attgctacag
      541 gtgcccatcc aagtattcct aatattcctg gtgctgagct aggtggctct tctgatgatg
      601 tatttgcctg ggaagaactt ccagagtcaa ttgccattct aggcgctggt tatatcgccg
      661 ttgaattagc tggcgtactc cacacttttg gtgtcaagac agatctcttt gttcgccgcg
      721 atcgtccttt acgtggtttt gattcctaca tcgttgaagg tttggtcaag gaaatggaaa
      781 gaacaaactt accacttcac actcacaaag tccctgtcaa gttagaaaaa actactgacg
      841 gcattaccat tcatttcgaa gatggtacta gtcacacagc tagccaagtt atctgggcta
      901 caggtcgccg tccaaacgtt aagggcttgc aacttgaaaa agctggagtg actctgaacg
      961 aacgtggctt tatccaagtg gatgaatacc aaaatactgt tgttgaggga atctatgctc
     1021 taggtgatgt aacgggcgag aaagaactga ctccagttgc aatcaaggcc ggacgtaccc
     1081 tatctgaacg tctctttaac ggaaaaacta ctgcaaaaat ggattactca actattccaa
     1141 ctgttgtctt ttcacaccct gctatcggaa ctgttggttt gacagaagag caagctatta
     1201 aagaatacgg tcaagaccaa atcaaggttt ataaatcaag ctttgcatct atgtactctg
     1261 cttgcactcg caaccgtcaa gaatcccgtt tcaaactcat aacagctggt tcagaagaaa
     1321 aagttgtcgg acttcatgga attggctacg gcgttgatga aatgattcag ggatttgccg
     1381 ttgctatcaa aatgggagca accaaggctg actttgatgc aactgtggca attcacccaa
     1441 ctgcatctga agaatttgta accatgcgtt aatcgaaaca aaagaagctg atataaatat
     1501 gtctgcttct tttttaatga ttttcgaatc aacaaaaaaa ttatccaact tgactagtca
     1561 cgaaaatcgg ctgttacagt ttttgttacc ttgtttttaa aaaatggttg acatcgactc
     1621 ctatatgagt ataatagtta ctatacatta gaaaagaggt taactatttt gaaaaaagct
     1681 cacgtttatg ctatccctgc tattggggct gctctcattg ctgctttggc acaaatcagt
     1741 cttccaattg gacctgttcc cttcactctg caaaactttg caatcggctt gattgctact
     1801 gtctttagac cgagagaggc tgtactttct gctggactct atcttcttct aggtgctatc
     1861 ggtcttcctg tctttgcagg aggtggagct ggttttcagg ctttagttgg ccctactgca
     1921 ggctatcttt ggttttatct cgtttactct ggacttactt cctctctaac caacagcaag
     1981 agtggtgttg ttaagatttt tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc
     2041 gggattctca gcttgcattt cctagctgga atggcatttg aaaaagctct tgctgtgggg
     2101 gttcttccct ttatcattcc agaccttggc aaacttctgg ctattagttt tattagccgt
     2161 cccctacttc aacgccttaa aaatcaggct tactttacta actaaaaaag gatatcgagt
     2221 tatcatgact caatatcctt ttcttttatt ttgaaaactt atactcaatg aaaatcaaag
     2281 agcaaactag gaagctagcc gcaggctgct caaaacactg ttttgaggtt gtggatgaaa
     2341 ctgacgaagt cagtaaccat acatacggca aggcaaagct gacgcggttt gaagagattt
     2401 tcgaagagta ttagttgcct ttaaaggcat ccaccatcgt ttgaaattct tcatttgaga
     2461 gagtaatccc tttgcccatt ttagtatggt ctggactcca agcacgaata tcaaactttg
     2521 caggggcacc attaaagctc acacggttaa tttccttggt ccaacctttt tcgttttcag
     2581 aaagagtcaa caagtgctct tcgatttcaa atgtaaattc tgccattttc ttctcctttt
     2641 ttagtttcat tagtttattc gtaaaatctt gtagatttta ggaaaatttt atataatatt
     2701 gatataaaag aagggaggcc aatatgagac ataaattcca gcaagttcta aataaaatac
     2761 atgatttttt aaatggatat gaccaacctg accagactga aaccaactcc cttacagcca
     2821 ctattgaaga ggctatccag aaacaaaccg ctgttcacct tatcttgtct gagacaagct
     2881 ttacaggtga catcatcaaa tatgatcagc aaggccagca aattatcgtg aaaaattttt
     2941 ccaaaaatgt gagccggatt atccgtataa gcgatattca acgcctgcga tttgtcccct
     3001 caactgtcca aacagcccaa aaaaatagat ttaagaaaga gtgagatgta gttgcttcat
     3061 cccactcttt ttcttagcga atttgttcaa aatgtaaatg aactgcaata tgatctccat
     3121 aaccacttct ttccaagtca cgttgtaaac gataggaaat gtagtgttct gcaatggtaa
     3181 tgtaacctgc gcccaataaa cgatgttcaa ccatagattg aatcatactg atagtcgcac
     3241 gttccacctt ggcttcttgt aaatccaaaa ctaccttctt agtgacttga gcaagatttt
     3301 gacgcaaatc atctgtcaaa acataaacag tttgggctgc cttcaagatg gcttggtaaa
     3361 tcttatctgg attaaattca gcaatttcgc cattacgttt gattacttgc ataggtttct
     3421 cctttattct ttgttttctt tgatttctgc cagcattttt tcttcttcta ctgtcagttg
     3481 ataatgttca agtaaatctg gtctgcgctc gtaggttttc tttaaactct cgtacaatcg
     3541 ccactgacga atcttttcat ggtggccact catcaataca tctggcacga ccatgcctcg
     3601 ataatcatag ggacgtgtgt actgaggata ttctaaaaga cctgaagaaa aactatcatc
     3661 ttggtggcta gactccttgc caatcacttc tggaatcagg cgaactgtag catcaatcat
     3721 ggtcatagct gccaattctc caccagtgag gacatagtcg cctagggaaa tctcatctgt
     3781 taccaaggtc ttaatgcgct catcataacc ctcatagtgc ccacagataa agattagctc
     3841 ttcctcttga gccaaatctt cagcataagc ctgatcaaac tgctttccag caggatcgag
     3901 gagaataacg cgcggatttt tcttttcaat agcatcaaag gaatcgaaaa taggttgtgc
     3961 tctgagcaac atgccctgac cgcctccgta gggctcatca tctacatgac gggccttttc
     4021 agcattttct cgaaaattat gatactggat atccaagagc cctttttctc gagcctttcc
     4081 aacgattgag tgctccagtg gagaaaacat ctctggaaag agggttaaaa tatcaatctt
     4141 catcgtctaa cccttctaag atttccacat cgacccgttt atttggaata tcaacattga
     4201 gaaccactgg tgggatataa ggtaaaagca aatcacgttt gccttttcgt ttgaccaccc
     4261 agacatcatt agcacctggt tgcaggattt ccttgatggt tccaaccaag ctatcaccct
     4321 catagacttc caaaccgata atctcgtgat agtaaaattc accatcgtct aggtcattca
     4381 aatcttcctc agcgaccttg agactgtatc ccttgtactt ttcgatagta ttgatatggt
     4441 acatatcttt gaatttaata atgtcaaagt tcttctgttt acggtggcta gcgatggtca
     4501 ctgtttggac aaactgatct ttttcatcaa acaaagccag ctcagctcct tttttaaacc
     4561 gttcttctgc aaaatccgtc acagacaaga ctcgcatctc accctgtaat ccctgcgtat
     4621 taacgatttt cccaacatta aagtagttca tcttgtctcc tgtaatctcc ttttttccat
     4681 cttattctga caattctcga ataatagccg caattttttc cgattctgac cattgtaaat
     4741 aatggtgatt ccctcctaaa atgagtttag tattggaagt ccaatattct gattctctgt
     4801 actctttttc tctataagtc tgacaaaaaa taaatacagg gacatgagat tctatcgaca
     4861 aattctcaaa atcttcctca gtaatctctc cagatatctg aaattctgga tcttgatttt
     4921 ccaactctaa gcctttttct tgcattaatt cccagatttt tttattcgtt tcaggactaa
     4981 atgttgcttg agttaagttc ttaaaataaa gttcaggacc acactcgtca atcagcctca
     5041 tctgctcttc catttctgga taaggatttt ctgaaaaatc agcaaacatg acttttttag
     5101 ttgtcggttc aattgctact aaagtctgac acttaattgg tttctcgagt aatttgcaag
     5161 ctaaaattcc actccaacta tgtacacaaa gtatatattc agaaatccct aattcttcaa
     5221 gtgcttcata aaccacatct gcaagattat ctagattttt tccagcttgg tcatgaatcg
     5281 gactcctacc tgtgttcgga aaatcaattg tcaaataacc aattgtagga ggaggttttt
     5341 caagtataag tgaaaaattt tcataacttg gtagcaaacc tgctccgttt agacaaacta
     5401 gcactttctt ttgcttttga taagcaacag agagactacc aatttctgta gatacttcaa
     5461 acctcttcat aaagaaatcc actgattcta tataatgaat tattaaaaat ccttatcctt
     5521 tattttatca cgttccaagg attttctcaa gttggaggaa ggggacaaca tctctacttt
     5581 cccttcaaga atccttccaa atcatgttta tgttggtaat taacggctgc ggttttgtct
     5641 ttctcaaaga tggttctagt taggtcaata tgcttgatag cagcaatagc gacggtgtag
     5701 tgaatgatat cagccagttc tctggcaagt tcctcgttcg aatcctatcc cttcttttcg
     5761 accagagcgc ctattcaaaa cctcggctac ttctccgact tcctccacta acttcataaa
     5821 gagaccttca tcagtccgag actgctgt
//
LOCUS       DAVHIU010000106         5732 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVHIU010000106 DAVHIU010000000
VERSION     DAVHIU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.108"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(88..160)
                     /locus_tag="VVN06_001715"
     tRNA            complement(88..160)
                     /locus_tag="VVN06_001715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Lys,seq:ctt)
     gene            complement(425..1696)
                     /locus_tag="VVN06_001716"
     CDS             complement(425..1696)
                     /locus_tag="VVN06_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5563210.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALTDLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            1966..2205
                     /locus_tag="VVN06_001717"
     CDS             1966..2205
                     /locus_tag="VVN06_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563211.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            2370..2549
                     /locus_tag="VVN06_001718"
     CDS             2370..2549
                     /locus_tag="VVN06_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563212.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            2686..2865
                     /locus_tag="VVN06_001719"
     CDS             2686..2865
                     /locus_tag="VVN06_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU5563213.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            2902..3354
                     /locus_tag="VVN06_001720"
     CDS             2902..3354
                     /locus_tag="VVN06_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5563214.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            3645..4115
                     /locus_tag="VVN06_001721"
     CDS             3645..4115
                     /locus_tag="VVN06_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU5563215.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNYAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDALMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPKY"
     gene            4134..5222
                     /locus_tag="VVN06_001722"
     CDS             4134..5222
                     /locus_tag="VVN06_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5563216.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            5203..5361
                     /locus_tag="VVN06_001723"
     CDS             5203..5361
                     /locus_tag="VVN06_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU5563217.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            5471..>5732
                     /locus_tag="VVN06_001724"
     CDS             5471..>5732
                     /locus_tag="VVN06_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5563218.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKG"
BASE COUNT         1734 a          965 c         1311 g         1722 t
ORIGIN      
        1 ttaagctagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa agccctatat
       61 tataagactt ttcctgttgt atttagatgc cccctacagg gatcgaacct gtgacccacg
      121 gattaagagt ccgctgctct gccagctgag ctaaggaggc aaagaaaaaa gctgtattgg
      181 tgccgaaact tcacggtttg tattgaaccc gcgcaattaa gcaggtgggc aactcgctct
      241 aactgaagct gtttccgtgt gagacggcct acatgctgtt agaagacttt tgtttcccta
      301 ataatacaaa aaatagtcgg tcaacactta agtgtgaagt cgtacaccac agcgtttcta
      361 tacttatatg ataccacttt ttcaaaaaaa atcaagagga aagtgcaatt ttttgaaaag
      421 gatttcagat ttttcgcaaa cggtcgatag cttcctttct ttgagccaaa gcccgttcat
      481 atttaccagt atcttctgct tggaaatagt gatgattacg aattttttct ggcagatagt
      541 cttgcttgac ccaatttcca ggatagttgt gtggatagag atagtcttgg gcattcccca
      601 gttccttgct tccactgtag tgcccatcac gtaggtgtcg cggaataggc aagtgccctg
      661 atgttttgag gtcagtaagt gccttatcca tagctacata ggctgagttg gattttggag
      721 aaagggccaa atcaatcacg acattggcaa tgagaatgcg ggcttctggg aacccaatct
      781 tctgggcagc atccagagca gtcacggtat gaatctgggc ctcaggattg gctaagccga
      841 tatcttcata ggcgataaca gtcaagcgac gagcgagact aggcagatcc ccagcctcaa
      901 tcaagcgggc agcatagtgg agactggcat ccacatctga gccacggata gacttttgca
      961 gggcagatag aacatcatag tgaccgtctc catccttgtc catagtgatg tagcttctct
     1021 gaagactatt ttccatgatg tctaaggtga tgtggcgaat gccctcgtca ttctcagggg
     1081 tagagagaac agccaagtcc agtgagttaa aggcagagcg aaggtctccg tttgtagagg
     1141 ttgcgatgaa atccagcgca tcctcatcta gttctattgg aaaatcaaaa ccacgttcag
     1201 ggttacttag agctatctgc agggcctctt tgacgtcttg gttagacaga ggttccaact
     1261 caaaaatttg aacacggcta cgaatggcag gagtgacgga aaagaaagga ttttcagttg
     1321 ttgctccaat catgatgacc agtccacttt ccaagagagg caagaggaag tcttgcttgg
     1381 tcttatctag tcgatgaatt tcatctagca ataggacgag accaccagaa aatttagctt
     1441 cttccgagat ttcttgcagt cgctttttac tatcaactgt cgcattgaaa gttcgaaagg
     1501 catacttggt cgttccagcg atggcagagg caatactggt tttgccgatt cccggagggc
     1561 catatagaat catggaggac aggcggttgg cttccaccat gcggcggatg atttttccag
     1621 gtccgaccag atgctcctga ccgatgacct ggtcgatggt tttagggcgc atgcgaagcg
     1681 cgagattgtc tggcatagta gtcctttcta gtgtggattt gagtagaatg actcatctac
     1741 tatatcttac tatgtccatt ctagcctatt agttgtgata tttcaatggt tttgtaataa
     1801 tcagggagtg agatagaatc ttttttacta tttaatttaa tgaggagagg tatcagcatg
     1861 agagatttga ttttccttaa agctcaaaat aggatagttg agcctaaaga agtggaagtg
     1921 ctgacttgag aaataatatt gctaaatgtc attgtatgaa ctataatgaa aagagaggtg
     1981 ataagcaatg gaaatgatgg accaagtcag gaaattttga tttttacaaa acaaattcgt
     2041 cattggattc ttagtgatca agttatttca gggaaaagaa aacttttctt ccgggaagat
     2101 actccaaaag agattttaga tttgtacgaa aacattaaat ctaaacttga ttttgcttac
     2161 caagaagttt attctaataa tgggttgaaa aaatatgaaa aatagaataa ttgatgtttt
     2221 tgaagtagta aatcgcatcc ttgtaataac tgttgaaaat cctgatttcg aagacttgag
     2281 agttaatcat actcaatgaa aatcaaagag caaactagga aactagccgc aggctgtact
     2341 tgagtacggt aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag tatcagtttg
     2401 taaaaattgg agataaaaaa tatagagtac gcagtgttcc tatgattcat tcaactccgc
     2461 ctcaatctgt cttagatcga gatactttta caattgacta tacagatgat gaattgctcg
     2521 ataaagaagc agtttttact actcattaga aaaataggtc tgaataaatt ggcttccctt
     2581 agaaccacat ctaggggctt ttatgtataa tagaataata agtatcactc ctttacaata
     2641 cgtgtaacac gtgttataat ggtgttgtca ggaggtaaaa gcgctatgcc tatgacacaa
     2701 aaagagatgg ttaaactcct tacggcccat ggttggataa agactagagg cggtaaaggc
     2761 tcccatatta aaatggagaa gcaaggggaa agacttatca caatccctca tggtgagctg
     2821 aataagtaca ctgaaagagg gataagaaag caagctgggt tgtaaaattc agcccctgtt
     2881 ttttgatatg gaggtagtga aatgttagtt acgtatccag ctttatttta ttatgacgac
     2941 acagacggaa cagaagcgac ttattttgtc catttcccag attttgaata ctcagctaca
     3001 caaggagagg ggatttctga ggctttggct atggggtcgg agtggctagg gataactgtt
     3061 gcagatttga tagagagtga tggagagtta cctcagccat cagatatcaa tagcttgtct
     3121 ttaattgata atgatccttt taaagatgat gaagatttcg tgtcaaccta tgaccttgat
     3181 aaatctttta tttctatggt atctgttgat gtatcagaat acctaggaag tcaggaaccc
     3241 attaaaaaga ctttgactat accaaaatgg gcagataagt tgggacgaga aatgggactt
     3301 aacttttctc agactttgac agacgctatt gcagataaga aagttcaagc ctaagattaa
     3361 gcgtggtatc gtggtaatag tcaataaaaa agcacgtcca cttgtgctag ttactcgcct
     3421 attgatttta tagatttatt agccctttcg agggcttttt atattgattt ttatgagaaa
     3481 tgaagaaatc agacttttaa gaaaatcact gataccaagg gtttaaatga ggtaatatgg
     3541 tataattagg acataaaata attttgtggt aagatggtag tatctatttt agcatatttc
     3601 cgagcaatcg gggcgattaa agagtcgcat agaaagagga caaaatggca acatacggat
     3661 ttttagatgt tttagaggaa gagttggaca agaactttcc ctttgacttt gagattagct
     3721 gggacaaacg caactatgcg gtagaagtga gttttctatt ggaagcgcaa aatgctgcag
     3781 gtgtggagat ggtggatgaa gacggagagg tttcatcaga tgacattctt tttgaggaag
     3841 cggtcctttt ctacaatcct gccaagtcaa cagtcaatga ggaagactat ttgactgtta
     3901 ttccttacct gcctaaaaaa ggattttccc gtgaattttt agcttatttt gcgcttttcc
     3961 ttaaagatac tgctgaggtt gggctagatg ccctcatgga ctttttggaa gatccagaag
     4021 cagaggaatt tgttatggaa tggaatcaag aagtctttga agaaggaaaa gttggcttgg
     4081 aagagggaga attttaccct tatccaaaat actaggagtt ggttggaggt gttatgaaga
     4141 aaattgggac ttatttggtt tatgtgctag ctgttgtctt tattatgcta gcttttgctt
     4201 gtggaacaat cgcatttgca gagttggggt attccgcagt tttagccttt acttttggtt
     4261 atgcctttgc tcttctaagc atgtatttaa tctttattct tcatgagctg ggtcatgctt
     4321 tttgtggcta cttgacaggc tatcggctgg tggcttttgg attaggacat tttattttga
     4381 ccaaaaagtc aggcaggttt catcttagta gaacagccat tctgaaaaat gttggtgctc
     4441 aatacattgg tttaaaagag gatgaaagcg atcaaagaat catcctgatg ctttcaggag
     4501 gcttgatggt tcatctcggc ttgatattat tggcgatatt gtttggattt ttgacaagaa
     4561 gctggtattt tgcagggact tggatttttc ttaatttatc tttcttccta aataacattt
     4621 taccagtcga catcaccgat ggagcaaaaa tttgggaatt actacaacac cctgaaaata
     4681 ccaaatacgc ctacctggta ttgagacatt ctgcccagac cttgctggct cctcaagaat
     4741 atgatttgaa agactttgtt caagcagttc ctgaggatgc acgagggagt tttgcggatg
     4801 gtgtattagg tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa gtagctaaac
     4861 agcagtttca agcattactt gagaggacaa ctactccgat gatgcagaca gttgcccaac
     4921 tatccctgct tcatatcgcc ttgttggaag gagattttga gcaagcggag caatatgcca
     4981 gtattcgaca ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa gtgatacaag
     5041 cctggtatca atttaaggta aggaaagatg tggttcaaac tcacaaggct atgaagattg
     5101 ctagacagaa aatggatagt agtcgaatgt tgagggacga gaaacgctat tatgaaaact
     5161 tgttagcaga gctggaaaag gaaatgttag aaggaactga agatggaact agaaatttct
     5221 gatttcccag gctgtaagat tgctttgttt tgtggggata agcttttgac tatcttacgc
     5281 gatgataagg caagcattcc ctgggccaat atgtgggaac tgccaggtgg gggaaagagt
     5341 gtcagggaaa atctgatata gttttgcatt aaaagaatat cctttcaagg ttattctttt
     5401 ttaattttgt cccaaaataa ttgactttgt cccgaaaata tagtattatt tgtataagga
     5461 ggaatgaatc atgaaaaaat cagatgttct tgatttaata aagtatcatt atgagggcag
     5521 agagacagaa tttagaaatc aatccatagc aattgctcgg aacttcaata aacatggtga
     5581 tacacaaata gcacaatata ttatggggtt aatgtctcaa agtgatagat tcatgccaca
     5641 aatagaaaat cctagtgagt atttgactcc tgctaaacta gatattggtc ctctcccatt
     5701 gcctctttca attatgaatg atttaaaagg aa
//
LOCUS       DAVHIU010000107         5700 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVHIU010000107 DAVHIU010000000
VERSION     DAVHIU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.109"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(426..1274)
                     /gene="dprA"
                     /locus_tag="VVN06_001725"
     CDS             complement(426..1274)
                     /gene="dprA"
                     /locus_tag="VVN06_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU5563219.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1370..2059)
                     /locus_tag="VVN06_001726"
     CDS             complement(1370..2059)
                     /locus_tag="VVN06_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5563220.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2071..2949)
                     /locus_tag="VVN06_001727"
     CDS             complement(2071..2949)
                     /locus_tag="VVN06_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5563221.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2939..3808)
                     /gene="licA"
                     /locus_tag="VVN06_001728"
     CDS             complement(2939..3808)
                     /gene="licA"
                     /locus_tag="VVN06_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU5563222.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3825..4847)
                     /locus_tag="VVN06_001729"
     CDS             complement(3825..4847)
                     /locus_tag="VVN06_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5563223.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4852..5559)
                     /locus_tag="VVN06_001730"
     CDS             complement(4852..5559)
                     /locus_tag="VVN06_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5563224.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
BASE COUNT         1809 a         1189 c          962 g         1740 t
ORIGIN      
        1 agtgtattga atctataaca gtacaccttg gctgctaaaa tatttctata aattaatttg
       61 actttcctga tagagttgtt cacatcttat ttcaattcac tatactttcc cttatactca
      121 atgaaaatca aagcgcaaac taggaagcta gccacaggtt gctcaaagca ctgctttgag
      181 gttgtagata agactgacga agtcagctca aaatactgtt ttgaggttgc agatggaagc
      241 tgacgtagtt tgaagagatt ttcgaagagt attatatgaa aaaatcctct actctactaa
      301 tccacttaga aaaaacgcat attatcaagt gttaaaaact tgctaatatg cgttttatta
      361 tggaaatgtt gggttcattt tctcttaaaa ttgatttttt cttagaattc tagcttaggt
      421 catttttaaa attcaaattc cgcaagaaca tcttgcccac tggtgaccaa ttttgctcct
      481 tcttgaatca aatgatggca accgtctgat agtccatcta aaatgctacc aggaatagca
      541 aagacatcgc gtccttcttc cattgctcgc tcacaggtaa tgagactacc tgaacgcatc
      601 ttagcctctg ctacaatcac accacgacaa agtccagcaa tgatgcgatt acgggcagga
      661 aaatgaaatt tcagaggttg ttcgccaggt ccatattcac ttagagccag atggtcattg
      721 ccgatgtagt cttgcaagcg tttattggct ttaggataaa acacatccag tcctgttcca
      781 atcactgcaa tggtttttcc gccattctga agagctgcca tatgagctgc tgtgtcaatg
      841 cccttggcca gaccactgac aataaccagt tcattttcca agccttgaat gactttttca
      901 actgacttag ctccctgttt gctacaagca cgactgccca cgaccgctac cttcgggaat
      961 ttcaggaggt caagatttcc cttgtaaaat aaaagtacag gcgcatcata tatttcactc
     1021 aaatcccaag gataacagtc atctaaaata gagaaagatg gaaatttttg aaactctttc
     1081 gacaaatgcg catcgtctat ctgaaaataa cgttccataa aaacggctgg attacggcaa
     1141 cctgatatat ctgcaatatc acccaacaaa agctcctgat caacattttc accgtattct
     1201 agcactttca aaatctgttg attggtcaaa cctgattttt ttaacttata gatttcatag
     1261 tttgtgattt tcataaataa ctccatttct ttttctactc atctatctat tcgtaaaagt
     1321 tcgtaaaaaa tcaaaaaaca tccgactatt ttgtcagatg ttggaatctt taattttcgt
     1381 ttttaagaat ttcttctaat ttacgatagt cttggacact atcgatctca taaatgctat
     1441 tgccttctaa ttcttcaaca tagacatcta gctctttgat attatcctta accatattgt
     1501 cccaatagag atcaacaaat tcaccacttg cataagcctt gtcgataaag ctgacaatct
     1561 tttctgcagt tggagcatcc cagaaggata caccactaag gatgcgacct gccttgctat
     1621 caacaataat gtcttgaacc ttgtagtcat ctccatagac caagaaccat tcgttggtac
     1681 aatcttcacg ataaacacta aaataagtcg aacgtgtcaa atcattgcgg aacatatttt
     1741 taaagagata attgtcagca tcaataacat agctgttggc caattcttct tttacaagat
     1801 agagagagta aaagttattg tagtcagcgt atttatcatt gaaaacgagg cgaacaccgt
     1861 atttttcttt taaataatcg aattgttctt taagataacc aacgatgatg atgatgtcat
     1921 tgattccttt ttctttgaga aactcaattt ggtattcaat caaaggtttt tgattaacct
     1981 gaaccaaggc tttaggggta ttttcagtca taggacgcaa gcgagttccc aatcccgctg
     2041 ctaagataat ggctttcaca cgaatctcct ttattcttta ataataatat aaactccagc
     2101 aatgacgaca agtgacgtca taatggtcag catatctaat gatgttccca agaaaagagc
     2161 tgcgaacaag acagtccata ctacatagct cacgttcaag cctgtagcct tggctggttg
     2221 caagcgattg atagcgatat aataagccaa ataggaaatc atatcaaagg ctacaaagac
     2281 aatcatgaga cctagcaatt gtccattggc tactgcaaca aatgactgat gagagaagag
     2341 cacaatcaca agataggaca agaacgaagt tacttgacgg attaagaggg cttcgatttc
     2401 actcaattca ctttccatgg caaaagagct aagaacactc tcacttcccc atgcaatagc
     2461 acaaaccaaa gcacaaagaa tcccaatgta gaaagaatta acctgttcaa ccttataggt
     2521 ctgggcaata atccctccaa taatcaagac aatcccaaat acagtatttt tcgaaatctt
     2581 gtgcttcaaa aagaagaaag ccaatagaac tgaaatcgca gggtaaatag ccgatacaga
     2641 tgaagctaaa gaacttccga tatacttaac tgcataaaga ttggcctgca taccgatagg
     2701 gcctgctagc aaggctccta tgataacact gacattgcga atatttaaga aaattgagag
     2761 gcgaactttc ccttctttta ccaagagaaa agctagtaag ataaagatgc tcaaaaaatc
     2821 atgagttgca gccaccacaa agggtgacaa atctgtaaaa atcgaaaaga tataagcact
     2881 aaccgttaga cccaagcccc agaaaatacc tgagagaagg ccaaaaggaa ctccgttttt
     2941 acttttcatc tgaacctcca taagaagcca aacctttaat agctctttgg taacgattca
     3001 caccatagtc accaaaatct tcaccttgct cttccttata gacagtccat agactccaaa
     3061 tagtatcttg taaaatttta taaatagcaa tcttttcatg agaaaccggt gtttggtcac
     3121 tctcatagtg agataagaaa gtttcttcct cttgggaagt gaattcagac tctaaaaaga
     3181 gggcagccaa atcccacatt ggatcattca ttgatgaata ttcccagtca atcaaataaa
     3241 gtcgtccttg aggtgattcg ataaagtttt caggcaccaa atcgatatga caagattttc
     3301 tgtcaacacc taagtcagcc agtcttttct ctaaggagaa gactgcattt ctaacagatt
     3361 catagttggc ataaggaatt tgttcttcaa tcaaggattc gtattttttg atttcttcaa
     3421 aaggagcaaa ttctcctctt aattccttag cagacgtatg aatagtttgt aatattggag
     3481 taattttgtc gaacttggtc ttgattgacg ttgaatcaag cgtaatcgca gattcgatat
     3541 actcatttac tttgatacca gcttcaatat caaaaagata attttttaca tctaagccta
     3601 aatcctttag tagttcaaga ttgtactttt catcttgtcg attgataagc ttttctgtcc
     3661 ctttaccaaa gaatttaaca atgtattgct tatttgttgt tttggccaaa tagttttgat
     3721 tggtcattcc acccagttgt tcaacactga ggacttcctc ttcttgacta agtaaggaag
     3781 aaattttttc tttaatgatt ttctccacaa ttaacctcca gtacttatac ttcccactta
     3841 aacactgttt taaaggctgt gtttaaatcg gttgcaaaga cacgatgaat atctttaatt
     3901 tctcttacag gttcttctag ataaaggata tttttaagac gattggcaaa tttcttgact
     3961 tccatcattt ggatagcatt ttcaaaatca atgcgaccag aacgagatga cccaaccaaa
     4021 atcaagccct tttctaaggc atcgcgagta ttgagattga ctttatattc gctaactccc
     4081 atcatgagaa tcgttccctg aggacgaatg tagcgaatca agtcattaat agctggtcca
     4141 gtaccatcac caccacaaca ttcaaaagca tggtcaaagg ccaaatcttc aggaatatta
     4201 tccgtaatat agcattcttt ggcaaatgag aagagttcca acttttccca atgacgacca
     4261 ataaccacaa tctctgcttc tggcaaagta tagttgataa tattggcaac cacaaaagct
     4321 aaacttccat ctccaataac ggcgatccgc tcccgcttgc tatgagcaag agtcaataga
     4381 cgattcatag cgtgcatgcc cacactgaca aactctgtaa tggctgcaac cgtatcttca
     4441 atagcatcat aagccaccac acgatcttta gggagagaaa caaactctct cataaagcca
     4501 tcaaatccac tagacaagaa atgggtccct gtcatgtagt tttcatagaa ttcttcatca
     4561 ctctgcatag gagactgatt gggaatcatg acaacttttt gaccaacctc gtaggttccg
     4621 gtcgggtcag aaatgacggt tccacatgac tcgtgaatca ttgccattgg aagcttttta
     4681 ttcaaaatct tgggatcacg ttttccctga tagtaacgct gatccgcatg acagacagcc
     4741 atgtagttgg gacggataag gatatgattc tcttggtcaa tagcctcttc ctgatatttg
     4801 acattgataa acttaggctt agttagttga taaatttgat taatcatttt actagtcttt
     4861 ctcaatcata ctttttgcaa tcttcaaatc tgttacggtt gtaatcttca gatttgagta
     4921 ttcacctttg gccaaggcca catcttttcc tttgatcaca aagattttac atgcatctgt
     4981 caagatttcc ttctcttcat cagaaagaga tccataaagg tccatgaagt ccttgcaacg
     5041 gaatgtttga ggtgtttgtc cttgataaag gtgagcacga tttggaatat ctgtaataaa
     5101 ttgaccattg gtactttcaa cgatagtatc aaccgcttct accactgtgt ccactgcgtc
     5161 atgattttgg gcaagttgga tattgtcctg aatcatgcga agtgtaataa atggacgaac
     5221 agaatcgtgg gtaacaacga tatcctctgg agtaagcgga cgataagcat caatggcttc
     5281 aatgattttc ttaatacttg tattgcggtc agcaccaccc tttgtaatga tgatacgttc
     5341 cttataaaga ggaagatatt tatctacaag atcttctgca tgagaaaccc agtctccatg
     5401 aacaccaact acaatttttt caatacttgg ctccaagaca aatttttcaa ttgtatgaat
     5461 caaaataggt cgatcaccta gctctaaaaa ttgttttggc aagttactga tccccatgcg
     5521 tgtgccagtt ccaccggcaa gaattcctgc ataaatcatc tattttctcc tttgtcttac
     5581 taaaaaaact tattctctct attataccac aatctagtcc tatcataaaa gaaatactgg
     5641 tcctcccctt ctagaatagg agaattatgc agttttatta ttcaaagaaa ctagtccatt
//
LOCUS       DAVHIU010000108         5646 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVHIU010000108 DAVHIU010000000
VERSION     DAVHIU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.110"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(281..2617)
                     /locus_tag="VVN06_001731"
     CDS             complement(281..2617)
                     /locus_tag="VVN06_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5563225.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            2750..3499
                     /locus_tag="VVN06_001732"
     CDS             2750..3499
                     /locus_tag="VVN06_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5563226.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(3792..4406)
                     /locus_tag="VVN06_001733"
     CDS             complement(3792..4406)
                     /locus_tag="VVN06_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU5563227.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(5222..5491)
                     /gene="rpsO"
                     /locus_tag="VVN06_001734"
     CDS             complement(5222..5491)
                     /gene="rpsO"
                     /locus_tag="VVN06_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5563228.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
BASE COUNT         1724 a         1258 c         1090 g         1574 t
ORIGIN      
        1 aacaatagat agcatcttca ttttcaaact accaacctat ccgaccattt gttctagcaa
       61 tagaagagag ttaaaattac ttcaatctgt cgctttctct ttcttacatc aaacttcaaa
      121 tagtctaact acagatattt gaatctatta cctcaatcca agcaggcttg agaatcactc
      181 ttgggttcac cgatagataa tagacatgta ggccttgcac atcagatcta cgatagatcc
      241 atcgtataaa aaactgcaat ccacagattg cagtctttct ttattttttc gcttggtaac
      301 gactggtcag gatgaaaatc acggtaaaga ccaacatcat gacaccataa acaggtaatg
      361 tttgtcctgt taaggttgaa atttcaagca gtttttggat tcttgggaag agagctgtgg
      421 ctaggaagcc tcctactgac cagacaatca agaggacacg ccatagggtg aatggcatgc
      481 aggctctaaa tacggataag aaaccaattg accccaagag ataatagagg agagttgaga
      541 tttctaactc agaccaacct tggctactcc caaatatttt cacaaacagg acgctgaaga
      601 cgaccatgag agcgcttggt agggcacgaa gcatggattt tctgaggaaa tttggctcaa
      661 caggtttgat atttcgctca aaagtcagaa cgaatggtgg gaaaccttcc acaaactggt
      721 caatcatggt aatctggatt ggaatgaagg ggaaaatcaa gatccattca gaccgaccta
      781 gtaaagcact ggcgatacag ataactgcga gcaagaatga gtagatggtc tttatcaaga
      841 aaatcggggc gatgtgggca atgttattga ccacgcgacg accctcgaag agaatctcag
      901 gaacatcatt aaagtctgag ttcaagagaa ccagattggc aatctgacgg gttgctggat
      961 ctccctccgc catcacgata gaacaatccg cctcacgaag ggccaagata tcattaaccc
     1021 cgtcccctgt catagccgtt gtatgacccg cttttttcaa cgtttggatg atgagtttct
     1081 tttgatgagg ggaaacacgt ccgaaaatag ctgtctcctc cgccatggcc atcaattcct
     1141 catcggtgat ttttgagcaa tctacatagc tgtgatagtc cgcaaaacca gccttctggg
     1201 caatgctgga caccgtaact ggattgtcac cagagataat cttgagtccc acctcttgag
     1261 aacggagata gtccagcgtc tctgctgctc cctctcgaat ggggtccaag atttccagca
     1321 aggctagagc ctgaatatca gatggtttct gtggtttgtg atggtctaat ttctcctgac
     1381 tgagagctaa gaccaagaca cgtgaacctc tctccaaggc ctctctggct tctgggacct
     1441 cagaatccag caacatctca ggtgccccta agaaaactgt ccccaagcct tctaactcca
     1501 tagcccccca cttgcggtcg ctcgagaagg gaagattgga aatcatagga taagcaacat
     1561 ctcccacaaa acgctggcga atggcttggg cagttggatt cttatcctca ctatgggcca
     1621 tgtagctggt caagatgctg gcaatagcct cttcaccata cgtttccgtc aacggaagaa
     1681 cagcctccac ctgcatcttt ccttgggtga tggtacccgt cttgtccaga cagagcatat
     1741 ccacgcgcgc caaggtctca acagagtaca tctcctgcac caagaccttt ttcaagccca
     1801 acttaatcac tgcagtcaag agcgaagtaa tggtcaaaag ggcaattccc ttaggcaaca
     1861 ttcccaaaag agctgtcgac gagtttacaa cggatgactt gagaggcagg ccttttaaaa
     1921 gcaaggcttc cagcaagaga gccagaccaa agggaatgat aatcttccca gtaaaaccag
     1981 ccaacttgtc cagcgatttc atgatacggg agttgatggg tttaacggtc ttggcttcca
     2041 gcataagttt ggcagcatag ttgtctgcac cgacatggtg aacttgagat aaaactgacc
     2101 cactggctag gaaacttcct gacaaaagta agccgtcaac ttccttttgc accaaatcac
     2161 tttctcccgt taacatggct tcattgactt ccgcaaagcc ttccaaaacc aaggcatcac
     2221 taggaatctg ctctcctgca gacaaacgaa tgacatctcc tagcactaat tcttcaggat
     2281 taagagcaac ttcctgacca tcacggatgg ttttgacctt ttccttggtc atgagattga
     2341 gcttgtccac catgtgtttg gctcgtagct cggtcacaat cccagaaaaa gcgttaaagc
     2401 agataacagc aaagaagacc agattgctcc aagcctgcac aaaggcaagg gccaaagcaa
     2461 tggcaaagtt caaagcgtta aaaagggtaa agacatttcg tttaacgatt tgccaagtgc
     2521 tggtactggc tgatgcggta aagtcattga ccaaaccctc agcctgtctt tccttgactt
     2581 ctctttgggt taatcccata atcttatttt tatccataaa ttctattata tcattttttt
     2641 taacagaatt ctaatttcat cccaaatatg catctagtca agggaaaagt ttgccagccc
     2701 tcctttgata aggtataata ataacaagaa aacaatcgaa ggagatcgca tgttttatac
     2761 ttatttgcgt ggattagttg tattgctctt atggtccatc aatggcaatg ctcactatca
     2821 taatactgat aaaattccta atcaagatga aaattatatt ttagttgcgc ctcaccgtac
     2881 ctggtgggat cctgtttata tggcctttgc gaccaagcca aaacagttca tctttatggc
     2941 aaaaaaagaa ctctttacca accgtatctt tggttggtgg attcgtatgt gtggcgcctt
     3001 tcccatcgac cgtgaaaatc ccagcgcctc agccatcaaa tatcctatca acgttctcaa
     3061 aaaaagtgac cgctctctca tcatgtttcc aagtggtagc cgccactcaa acgatgtcaa
     3121 ggggggcgca gcactgattg ccaaaatggc caaggtccgt atcatgccgg ttacctacac
     3181 cggtcccatg actttgaagg gcttgattag ccgtgaacgt gtcgatatga actttggaaa
     3241 tccaatcgat atctcagata tcaagaaaat gaatgatgaa ggcattgaaa cagtcgccaa
     3301 tcgtattcaa acagaattcc aacgtctgga cgaagaaacg aaacaatggc acaatgataa
     3361 aaaaccaaat ccactctggt ggtttatccg catccctgcc ctcatccttg ctattatcct
     3421 cgctatccta accatcatct ttagctttat cgcaagcttc atctggaacc cagataagaa
     3481 aagagaagaa cttgcataga agaaatgaac cttggccaaa cagctaaggt tttcatttat
     3541 atatcaaatt gaaactagaa tagtacacct ctacttctta aaacattttt agaaatcgat
     3601 ttgactgtcc tgatcgattt gtcctgtttt tgtttcattt tactatattt ataagaatct
     3661 cctaatcaaa tcaaaagcca ctctacaata gagtaacttt aagattgtcc atttatactc
     3721 aatgaaaaag ccctctgaaa atcagagagc tgatttgagc tcgggctaaa atcctagtga
     3781 aacaaaaaaa tctacacgat cagaaaagtc tctatcgtgc cattttcata catgatgtat
     3841 agtccaagta gaatgaatac taagggcaca atgattcgct cgtatttttc aattgtctcg
     3901 aatattaacg gaatagagga taacacccga ctaatctcgc aaaagataat tatgccgatt
     3961 acaaacacaa gcaaggccac gagggtctgt gaccaatcta acgaagcaaa ataaggtata
     4021 tagataccta aattatctcc gccagacgca attgtcagca atgtaactgt ccaaaacagt
     4081 tgatttgcct tgctttgttc taatctttca ataatttctt cctcttcttc ttccgcatct
     4141 tctccaacaa ttgcaaagcg aatccctaaa tagataggga ttaaaccaag caatccaacc
     4201 atccattctt caggcacgaa attaacgaca taagcagcaa ctaaactcgc ccctacaagt
     4261 aagcctgtgc ctagatattg ccccgcataa atatgccatt tctgtttatt ctgtgatagc
     4321 tgtgcaaata aaataattaa aataattaaa taatcgatac tggtggaaat ataaacacca
     4381 atagcagata tgattgtctg tcccataaaa aaaacctcct gtattagtca gtaataaatc
     4441 agcagatttt aggagagcac agacacatta atttagctat cgctagtggg taatgtcgaa
     4501 cgtaggaaag cattttactc ctcctcaaaa cgtagttaca aagtaatttc taactggaat
     4561 tcagtgacta cttccatgta aagtataaca tactatactt tactttgtaa agagtgggct
     4621 cttctagggg gcttgcatac agctactcgg cttgaaaagc cgagtaactg cgcaccttta
     4681 tggtgtaatt agctgatacc atttgaggtt tttgtagtct ccaaaattgt taccgataaa
     4741 acctcaataa aaaagccctc tgaagtcaga gagctgattt tagcccgggc taaaatccta
     4801 atgaaaaaga tgaaactcct tgtgctcatc gaacactgtg tcatttccct attttcatac
     4861 ggatttttga cgcccttagc atcatgattc ttgctagata aatcgtttat agactaggcg
     4921 acgaggcgaa gatcgtacga aactttcagt gaaacaaaaa aaatctcccc gaagggagaa
     4981 aagctagttt attttacctt acagtgctag gaaccgtaac cgaagattat acttgagtat
     5041 agcgaggtaa ggtgacaacg caaaaaagct acccaattta gagtagcttt attatcaaga
     5101 tatgctcaat tgaacacggc ctaagcactt ggcaaaaaag ataaaatctc ctagaaactg
     5161 aagtttcttc gtcaattttc ctatttttgc tttgtgcttt tgacagcctt tgtatcttga
     5221 attagcgacg aagtcctaga gagttgatca actcacggta acggttaacg tcgtttttac
     5281 gcaagtatgc aagcaagtta cgacggcgac cgattttttt catcaatcca cggtaagtag
     5341 cgtggtcttt tttgtgttgt ttgatgtgtt cattaaggtg gttgatttcc caagtaagga
     5401 cagcaacttg aacctctact gaacctgtat caccttcgtg acgtgcatat tgtgcaatga
     5461 tttcattttt tttctctttt gagattgcca tgatgtttct cctttttatt tggcttcatc
     5521 cgagtgacag gttggcatgc ctgtaaccaa gaagaagtta tttgtcttta cgacaatccc
     5581 tattttacca agatttaaat tttctgtcaa acattaatac tctcatagtt attatttttc
     5641 aagtaa
//
LOCUS       DAVHIU010000109         5547 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVHIU010000109 DAVHIU010000000
VERSION     DAVHIU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.111"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(147..2144)
                     /gene="metG"
                     /locus_tag="VVN06_001735"
     CDS             complement(147..2144)
                     /gene="metG"
                     /locus_tag="VVN06_001735"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5563229.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2268..3527)
                     /locus_tag="VVN06_001736"
     CDS             complement(2268..3527)
                     /locus_tag="VVN06_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563230.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3529..4230)
                     /locus_tag="VVN06_001737"
     CDS             complement(3529..4230)
                     /locus_tag="VVN06_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563231.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4214..5413)
                     /locus_tag="VVN06_001738"
     CDS             complement(4214..5413)
                     /locus_tag="VVN06_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5563232.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPATGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
BASE COUNT         1592 a         1271 c         1004 g         1680 t
ORIGIN      
        1 catgttttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt tttgagcaac
       61 ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaataa aaaaacagaa
      121 ctagcctgaa ctagtcctgt ctacttttac ccaatcacac ttccatttgg tacagctgga
      181 tcaactgtga gaagggttaa tttgccatca tgttcagctg agagaatcat accctggctg
      241 acatattttt tcatcatttt acgtggtttg aggttagcaa cgatttgaac tttcttgccg
      301 accaattctt gttcatttgg atagtatttt gcaattcctg aaagaatctg acgatcttct
      361 ccatcaccag catccaagcg gaattgaagc aacttatctg aaccttctac tttagacact
      421 tctttgactt ctgccacacg gatttcaacc ttgtcaaagt cttcaaactt gatttcatcc
      481 ttgtttagtt tgagctcaac ttcgtccgga ttccattctt tttcgactgc tggtttattg
      541 ccttccattt gttccttgat ataggcgatt tcttcttcca tatttagacg tggaaagata
      601 ggtgttcctt tggcaactac agtcacatct gctgggaagt cagccaaact caagttttca
      661 agactagaaa cttcttccaa accaagttga gtcaaaactg cacgactagt ttccatcata
      721 aatggttcaa tcaagtgagc aactacacga atgctggctg ccaagtggct catgacactt
      781 gccaattggt cacgaagagc ttcatccttg tccaagaccc atggtgcagt ctcatcgatg
      841 tatttattgg tacgagagat cagagtccag actgcttcaa gcgcacgtgg atagtcaact
      901 gcttccatgt gtgtatggaa gtctgcgatt gattgttctg caacctcagc aagaacatga
      961 tcaaattcag tcacaccttc tacataggca gggatttgtc catcaaagta cttattaatc
     1021 atggaaaccg tacggttaag gaggttccca aggtcattag ccaattcata gttgatacgg
     1081 ccgacatagt cttcaggagt aaaggttccg tctgaaccaa ctggaaggtt acgcatgagg
     1141 tagtaacgaa gtggatctag tccataacgc tctaccaaca tttcagggta aacgacattc
     1201 ccttttgact tagacatttt tccgtctttc atgacaaacc aaccatgggc aatcaaacga
     1261 tcaggtaatt taacatccaa catcataaga aggattggcc agtagataga gtggaagcga
     1321 aggatatctt ttcctaccat atggaagact gttccattcc agaacttgtc aaagttacca
     1381 tgttcgtctt gagcgtagcc aagagctgtc gcatagttaa gaagggcatc aatccaaacg
     1441 tagacaacgt gttttggatt tgatgggaca ggcactcccc atgtaaaggt tgtacgagat
     1501 accgccaaat cttccaaacc tggctcgatg aagttgcgta gcatttcatt aaggcgacca
     1561 tctggcgtga taaattcagg atgagctttg aaaaattcga ccaaacggtc ttggtatttg
     1621 ctaaggcgaa ggaagtatga ttcttcagaa acccattcaa cctcatgacc tgatggagca
     1681 ataccaccag tcacatttcc agcttcatca cggaaaactt ctgccagctg gctttctgta
     1741 aagaattctt cgtctgatac tgaataccaa ccagagtatt cacccaagta gatatcatct
     1801 tgagcaagta agcgttcaaa gacctgtgca acaacttttt catggtagtc atcagttgta
     1861 cggataaatt tatcgtatga gatatctagt aattgccaga gttctttaac tccaaccgcc
     1921 attccatcaa cataggcttg aggtgtaata ccagcttctt ccgctttctg ctggattttc
     1981 tgaccatgtt catcaagacc tgtcagataa aatacatcgt agcccatcag gcgtttgtaa
     2041 cgtgctagga catcacatgc gatagttgtg taggcagaac cgatatgaag tttcccagat
     2101 ggatagtaaa tcggcgttgt aatataaaaa ttcttttcag acataatttt tcctttccag
     2161 gcaaatgaaa cctgtttttc taacacttca ttatatcaca tttttaatga atttcgatag
     2221 ggaaatccat acaaaaacaa gatagacgag tgtccatctt gttgatctca ttcataacga
     2281 agggcttcaa ttggatcaag tttcgatgcc ttgttggctg gcaagactcc aaaaatcata
     2341 ccaacactag ccgaaactgc aagactaaat agggcaactg ggattgatac tccaacttct
     2401 ataccttcta ttaaaccttg cagtaacaaa cctgctaagg cagttaaacc acttgcaatt
     2461 gtcaagccaa ttaagccacc taacaaggtc aaaatcatgg attcaatcaa aaactgaatt
     2521 aaaatattgg cacgtgttgc gcccaaagcc ttacgaagac caatctcacg agtgcgctct
     2581 gtcaccgaaa ccagcatgat gttcatgaca ccagttcctc caacaaagag agaaatccct
     2641 gcgatggaac taataatcgt cgtcataaaa ctaaacgatt gttgaatttc tgcaaataca
     2701 acggactcat ctgccacctg gtattctccc tgttgtaagc ctgcaagctc tgtcattttt
     2761 cgtgccaatt ctggacccag agttggggtt aaactggtat cattcactcg aaagacaata
     2821 ttagctattt catctacatt aaaattcgca gcaagggaga tattggtagt aataggcaag
     2881 ccaccaaacc catatatttt tgatctttta gcctccggac tagtataaac cccaatgacc
     2941 cggtaactaa atccattgac ttctacaacc ttgttaatag cctcttgagg agattcaaat
     3001 aaactaatgg acaattcctc atctagcaaa atgacacttg caaactcttt gaaatcttgc
     3061 tctctcagac tacgacctgc aataatttca ttcttaacag cgtccatgta agttctgttt
     3121 ccacctgtca aattagcatt ctcaaccttt ttatcttgat aggtcaagat ggcattcgtt
     3181 gaattggtta catagtaact atccactccc ttcagtttag ctgcctcttg aacccaggat
     3241 tcttgcggtt ttggcggttc aacaggaact tcctcttcct ttccagaaac cgtaaaagct
     3301 gattgtttct gagtaaaaga cccatcttta ctttttttag gagagaaaaa gacgctaata
     3361 tttttctgag atttagtcat atctttattg acttgacgag atagggaatc acccaaagcc
     3421 ataatcacaa caactgatga aacaccgata ataatcccaa tcatagtaag caaagaacgc
     3481 atcttgtgag ccatgataga tgaaaaggca aatttcagat tctgcatctt agttttcctc
     3541 ctttcctaac tgagcactgt cagacgaaat gaccccatcc cgaatgacaa tctgacgttt
     3601 ggcataggca gcaatctcag gctcatgcgt taccatgata atggtttttc cttctttatt
     3661 caaatcaacc aataattgca taatttggtt acctgttttg gtatccaagg ctcctgtcgg
     3721 ttcatccgct aggataatag aaggattgtt taccaaggca cgcgcaatgg ctacacgttg
     3781 cttttgacca ccagataatt ctgaaggtaa atggtgacta cgttctgtca attcaacctt
     3841 gtctaaatat tcctcagcca acttgcgacg ttttgaagac gaaactcctg cgtaaatcaa
     3901 gggcaattct acattttgca gagcattgag cttcgataga agaaagaact gctgaaagac
     3961 aaaaccgatt tgttggttac ggaccttagc cagttgtttt tcaccaagcc cagccacttc
     4021 ttgaccttca agataatatt ctccactggt tggtgtatcc aacatgccaa tcgtattcat
     4081 cagagtggac ttaccagacc cagatggtcc catgatggct acaaattcac cctcattcac
     4141 ttctagattg atatttttga gaacctgcaa ttcttggtca ccattacggt agcttctgaa
     4201 gatatttttt agactaatta gttgcttcat cagtcttcac ctcttttcct tcttccaagg
     4261 aagatgttgg attactgatg accttagcac cattcgttaa accagaagtg atttcttgat
     4321 tttctgcgtc agcatttccc aatgaaacct caactttttt agccttttgt tgttcatcca
     4381 caatccagac ataattttta ctatcatcca ttactagact gctaacagga acaagaatag
     4441 ccttagtttt gcttttaacc tcaatgttga cagaaaaacc ctgtttcaaa tcaccaacct
     4501 cgcctgtcac atcaatagta taagggtatt tagaacctgt attattcccg gttgctggac
     4561 tagctgcttc accattgttt ttaggatagt cagaaatata gcttaatttc ccagtccatt
     4621 ttttatcagg atacacttta gaagtaaagc ttacttcttg acctacagaa aggttggcta
     4681 gattgtactc agacaattct cccttgactt gtaaattttc attgctgaca atatgaacca
     4741 taacttgact cgcccctgtt ggagatttag aaacattgct attgacttcg actacagttc
     4801 cctctagggt actgagaaca gttgttgcat ccaattgact ttgagccttg cttaattgcg
     4861 ctgcagcatc tgcacgcgca tcacgggcat cacccaattg agcatcaata gaagcaacag
     4921 aatttccagc cactggagtt gggctttgca ccgttgcatc ttctcctcct actggcgctg
     4981 gtaactgtgg agcctgagct gaagcggctt catttcgtgc ttgattgagt tcattgatat
     5041 gacgatctgc cttagctact gctcgactag ctgaatcata ggccgcctgc gcttctgaac
     5101 tactgtactt gactaaagcc tgcccttcgc tgaccttatc gcccacagaa acaaggattt
     5161 catctaaatc acccttacta gcatcaaaat aaacatattg ttcatttttt gctgttactg
     5221 tccctgacaa taaaacagag gatgccacgc ttccttcctt ggcaacaaca agatgagtag
     5281 gctcatcttt tacagcagtc tgagaaggtt gtctaaaaag taaaatcccc ccggcaccca
     5341 atacaactac actcgcagca ccgattgctg catacagttg ccacttttta gctttaccat
     5401 tctttttctt cataatgaaa ctccttttct tttttacaat actttgctat tataccaaat
     5461 ttccctccag caaacaatgc agttcagatg gaaataatgg cagttcagga ttaaacaatc
     5521 gttcggaatt ttgcttttct attgtac
//
LOCUS       DAVHIU010000110         5453 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVHIU010000110 DAVHIU010000000
VERSION     DAVHIU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.112"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            8..2038
                     /locus_tag="VVN06_001739"
     CDS             8..2038
                     /locus_tag="VVN06_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU5563233.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            2042..2326
                     /locus_tag="VVN06_001740"
     CDS             2042..2326
                     /locus_tag="VVN06_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5563234.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            2339..3685
                     /locus_tag="VVN06_001741"
     CDS             2339..3685
                     /locus_tag="VVN06_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5563235.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            3689..4546
                     /locus_tag="VVN06_001742"
     CDS             3689..4546
                     /locus_tag="VVN06_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5563236.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            4543..>5453
                     /locus_tag="VVN06_001743"
     CDS             4543..>5453
                     /locus_tag="VVN06_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU5563237.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSSSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVL"
BASE COUNT         1727 a          737 c         1151 g         1838 t
ORIGIN      
        1 aagagaaatg tttgattata gagcactagt tattttgatg actttgatgg atcattgtga
       61 gctatcatta tatgaattat ctgttaaggt gagcttacct ataaaggaag tcaaagaagg
      121 aatagattat ttagtgcctt atctagctaa taaagggata gtgctggata aaaaacaagg
      181 tcgctatagt ttatcaaatc gtacgaagca gtctttgaca gatattatta agtcggatga
      241 attggtttta ccaaagtcga ctcgcttagc tttaatctat ctttacactt tttgccgatt
      301 agattttata tcgaataatc attaccaaga ctttttgaaa gtaagtaaga atacaacctt
      361 atctgatatt caatcattaa gaaagattat gttagataat gatttggagc tagggtacag
      421 tagagcaaaa ggttatactt tacacggttc agagtggaat aagcaccgtt tagcttttca
      481 aatggttagt gagttgctgg aatcctccat agggatttgg ggtttggatt atgttttatc
      541 cagttgggga tattcattaa cttatgattt gattgatcag gtagttaaag attattatga
      601 gaagctacag ctagtaccta ttgttaatca attaaaagtt taccttttcg gtttagtatt
      661 cattctgtgt aggtatcaaa gggatgttga aagagtatgt ctttcagaga cattagtatc
      721 tcctgttatt caagatataa cgactatttt attggataca gtggttgatt tgggaattat
      781 agatacggtg ttttctgagg atgattatcg ctatatcaca gttttattat caagttgctt
      841 tgaaggtgaa gtagatgttg ctccggttta ctttaatcaa ttaacagagg ctattataag
      901 tagaatggaa gatatttctt tgttacattt taaacaaagg gaaggattga gagaaaatct
      961 tcgtcgccac cttataccgg cttactatag attaaagttc ggcctaccta gttcaaatga
     1021 atatgtattg catgttaaag aacattatcc tgatttattt gaattagtca aagattcttt
     1081 gatgcccttg atggacgcta ttgacaatcc catacctgat agtgaaacag catattttgt
     1141 tatccatttc ggaggttatt taaagaaagc agatactttg cctcaaaaat ggtataaagc
     1201 agcaatcatt tgtcctaatg gtgttagttc ttcattgatg ttaaaagaga atctattagc
     1261 attatttcct caaattgagt ttataggaac ctcaaagatt gatgatttac aggtgaaagc
     1321 tagtagtgac tatgatatgg ttttttctac cataaaggtg gagacagaaa agccaaatta
     1381 tctagtttcg gttatgatga ctgaagagca agcaatacaa ctagtagaac tagtgttaaa
     1441 agattttccg aatttagagt atggagattt tgagattgaa caaatcctga atattgtcaa
     1501 aagatatgga attattacac aagaattgga attaagattg gcgttaaaga attatcttta
     1561 tcaaaaaaat gatagaaagg aaattgtacc agtgctagaa caacttatta ccaaagaaac
     1621 ctatcaggtt agttcgcaaa aattaggatg gaaggaggcg attcgtttag cagctaaacc
     1681 gcttttggat caagataaga taacggagaa ctaccctgag gcaatgattc aaaaagtaga
     1741 agagtttgga ccttttatta atttagggaa gggagtagca attcctcacg ctcggccaga
     1801 tgaaggtgtg aatgaaatag gaatgtcaat gttagttttg gaagaaccga tttatttatt
     1861 ggataatcca gaacaagagg taagattgtt gatttgtatt gcagccattg ataatgagag
     1921 tcatttaaag gctttgtcac atttaacaac aattttaaga gataaaaatc atgttcaaac
     1981 tttaatttca tcaaaaaact atgatgacat taaaatgata attaaacagg aggattagat
     2041 aatgttaaaa attggtacag cttgtggttc aggattaggt tcaagtttta tggtacagat
     2101 gaatattgaa tctgtattga gtgatttgaa tgtttcggat gtagaagttg aacattatga
     2161 tttaggtgga gcagatccaa atgcagctga tatttggatt gttggtcgtg atctagctga
     2221 ttcagctagt catcttggag atgttcgtat cttaaatagt attattgata tggatgaact
     2281 acgagaatta attactaaac tttgtgaaga aaaaggactt atataggagg ctagcgttat
     2341 gatgaagttc atattggata ttgttagtac accagctatt ttagtagctt taattgcaat
     2401 tttaggatta gttcttcaga agaagaaatt acctgatatt attaaaggtg gaattaagac
     2461 ctttgttggt ttcttagttg tatctggtgg tgcaggaatt gtacaaaatt ctttaaatcc
     2521 atttggtacc atgtttgagc atgcttttca tttatctggc gttgtgccga ataatgaagc
     2581 aattgtagct gtagctttaa caacatatgg ctcagctact gcactgatta tgtttgcagg
     2641 catggtgttc aatatcttaa tcgctcgttt tactcgattt aaatatatct ttttaacagg
     2701 gcaccacact ctatatatgg catgtatgat tgcggtcatt ttatcagttg ctggctttac
     2761 tagcttgcct ctcatcttac taggaggatt agcactcggt attattatga gtatttcccc
     2821 agcatttgtg caaaaatata tggttcaatt aactggaaat gacaaggtgg ctttaggtca
     2881 tttcagttct ttgggatatt ggttgagtgg ttttactggt agccttatcg gtgacaaatc
     2941 aaaatcaaca gaggacatta aatttccaaa gagtttagct tttttacgtg atagtactgt
     3001 tagtatcact ttatctatgg cagttattta cattattgta gctatctttg cagggtcaga
     3061 atatatagaa aaagaaatca gtagtggtac aagtggtcta gtttatgctt tacaattagc
     3121 aggtcaattt gcagcagggg tatttgttat tttagcaggt gttcgcctta ttttgggtga
     3181 aattgttcca gcctttaaag gtatttcaga gcgtcttgta cctaattcaa aacctgcttt
     3241 ggattgtccg attgtttata cttatgcacc caatgcagtt ctaattggat ttatctctag
     3301 ttttattggt ggtttagtaa gtatggcaat tatgattgct tcaggaacgg ttgttatctt
     3361 accaggtgtt gtgcctcatt tcttctgtgg agcgactgca ggtgtcattg ggaatgcatc
     3421 tggtggtgtt cgtggagcca ctattggagc atttttacaa ggtattttaa tcagttttct
     3481 tccagtcttt ttaatgccag ttttgggagg acttggtttc caaggatcaa ctttctcaga
     3541 tgcagatttt ggtctatcag gaattatttt aggaatgtta aatcaatttg gctcacaagc
     3601 aggcattgtg attggtcttg ttcttattct agcagttatg tttggagtat cctttattaa
     3661 aaagccatct gcaaaggagg aataagggat gattttaagt aaaaatagag aagatgagtt
     3721 aagaaaattt gcgactaaca tccgattaaa tactcttaga acattgaatc atcttggatt
     3781 cggacattac ggaggaagtc tgtctatcgt agaagtttta gcggtgcttt atggtgaaat
     3841 aatgccaatg actccagaaa tatttgcagc acgagataga gattatttca tattatcaaa
     3901 aggtcatgga ggaccagctc tatacagtac actctatttg aatggtttct ttgacaaaga
     3961 attcttatat tctttaaata caaatggaac caaattaccg tctcatcctg atagaaatct
     4021 aacgccaggc atagatatga caacgggctc tttaggacaa ggaattagtg ttgcaactgg
     4081 acttgcatat ggtcagagaa taagaaagag tcccttttat acttatgcta ttgttggaga
     4141 tggtgagtta aatgaaggac aatgttggga ggctatacag tttgcttctc atcaacagtt
     4201 atccaactta attgtatttg ttgatgataa caaaaaacaa ttagatggtt ttacaaagga
     4261 tatttgtaat ccaggtgatt tcgtagaaaa attttcagca tttggatttg aatccattag
     4321 ggtcaagggt tcagatatta gagaaattta tgaagggatt gtccaattaa aacagtcaaa
     4381 taattcatca cctaagtgca ttgtattaga tactattaaa ggtcaagggg ttcaagagct
     4441 ggaagaaatg aaatccaatc atcatcttcg ccctactgta gaggagaaac aaatgttaac
     4501 ttcagttgta gaaagattaa gtcaggaatt ggaggaaaca gaatgatgag aagtacgaaa
     4561 gaattacggc atgtatatag agacttcctt ttagaggcta atcaaagttc ttctgatata
     4621 gtagtcttag aagccgattt gtcaagttcg atggctactc ataatcttga aaaggacttt
     4681 ggagaccgtt atgtgaatgt tgggattatg gaagcagaaa tggtcggcct tgcagcaggc
     4741 ttgtctattc aggggtttag accttatctt catacatttg gtcctttttc ttcacgaagg
     4801 gtatttgatc aattatttat ttctcttgga tacgcacaat tggatgccac tgtgattgga
     4861 tcagatgcag gagtaactgc agaaatgaat ggtgggacac atatgccatt tgaagaaatt
     4921 ggattgttac gtttaattcc taaatcgatc atttttgaag caactgatga tatccaattt
     4981 cgtgaaatat tgaaccagac attagactta aaaggactaa aatatattcg aacaattaga
     5041 aaagctccag ttcctgtgta tcaaggtgga gaagattttt ctaaaggcta cattgagtta
     5101 agacatggtg aagatcttgt aatcgttgct tctggtataa tggttgctcc aagtattcga
     5161 gttgcagatg aactgtctaa attaggttat tcagtaggtg tgatagattt atttagaatc
     5221 aaaccgatac cagaacagat aaaaacaatg ttaagtggaa aaactatatt tactgtagaa
     5281 aaccacaatc agataggtgg aattggcagt tctttatgtg aattattctc tgatgatgga
     5341 ataacaaaaa ttcatcggat gggtgttcaa gaaagatttg gtcaagtagg gaaaatggat
     5401 tatcttctta atgagtatgg tttgagtgaa tcgaatataa aagagaaggt tct
//
LOCUS       DAVHIU010000111         5293 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVHIU010000111 DAVHIU010000000
VERSION     DAVHIU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.113"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            36..1358
                     /locus_tag="VVN06_001744"
     CDS             36..1358
                     /locus_tag="VVN06_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5563238.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1360..3246
                     /locus_tag="VVN06_001745"
     CDS             1360..3246
                     /locus_tag="VVN06_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU5563239.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3414..3758
                     /locus_tag="VVN06_001746"
     CDS             3414..3758
                     /locus_tag="VVN06_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5563240.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3768..5180
                     /gene="lacG"
                     /locus_tag="VVN06_001747"
     CDS             3768..5180
                     /gene="lacG"
                     /locus_tag="VVN06_001747"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU5563241.1"
                     /translation="MENLQFKVLPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKLGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
BASE COUNT         1652 a          744 c         1136 g         1761 t
ORIGIN      
        1 ttaatttata aaaaataaaa aaaggagttc cggtgatgaa caatatttta gcgtttttag
       61 aaacaaaagt cgctccgttt ggtgaaaaag ttggcaacca acggcatttg aaagctattc
      121 gtgaaggatt tatgatggca atgcctttga ttttagtcgg ctctttattt cttattctaa
      181 tcagttggcc tcaagaggat tttacaaatt ggctgaatag tgttggattg ctaagtatct
      241 tgacaactat gaatcagtca acagtagcga ttatctcctt ggtcgcttgt ttcggtattg
      301 cctacaggtt gtcggaagga tatggtacag atggtccgtc ggcagggatc atagccttat
      361 ccagttttgt attgatggca cctcgttttt cgagtatggt ttatgataaa aatggggagc
      421 aggtcaagca gttatttggc ggcgcaatac cattttctag cctgaatgca tcttctttgt
      481 ttatggcgat tactattgga ttggttacag cagagattta tcgtatgttt atccagcgcg
      541 gaattacgat aaaaatgcca agtggtgtcc cagatgtggt aagtaaatca ttttcagctc
      601 ttttacctgg ctttactact tttgttttgt gggctctggt cttaaaaggt cttgaagcgg
      661 caggagttgc aggaggtctc aacggactcc taggtgcaat tgttggaaca ccgcttaagt
      721 taattgcagg aacgcttcca ggtatgattc tatgtgttat tgtaaactca ttcttttggt
      781 tctgtggagt taatggggga caagttttaa atgcttttgt agacccagtt tggttacaat
      841 ttactacaga aaaccaagaa gctgtggctg caggacaaac actccaacac attattacat
      901 taccgtttaa agatttattt gtatttattg gtggcggtgg agcgactatt ggtcttgcga
      961 tttgtctctt cctatttagt aagagtcgtg cgaataaaac attaggtaag ctagctatta
     1021 taccgtctat ttttaatatc aatacagcta ttctatttac gtttccaaca gttttaaatc
     1081 cgattatgct gattccgttt attgctactc ctacaatcaa tgccttgatt acctatgtat
     1141 caatggctgt aggattagta ccctatacaa caggtgtaat ccttccgtgg acaatgccac
     1201 cgattatagg aggcttcctt gcaacagggg ctagttggcg aggagctcta ttacaagttg
     1261 ttttgatttt ggtttctgta gcaatttatt atccattctt caaaattgca gataaacgca
     1321 atcttgaaaa agaaaaagct actgttggag ggaaataaga tggttatcag agtatttgat
     1381 caacagaaaa atacttattc tagctttgcc ttagaggaat taagttacta tatgaatcgg
     1441 gtctttaaga ctaacataga gcttgtcgag gagaaggaag cggatatttt tgtaggatta
     1501 gtcaataaag aggacagaaa agaccatgtt cttatctcat tagacaaggg taaggggaga
     1561 attgagtcta atacaattgt aggtttactt attggaattt accgaatgtt tcatgaattt
     1621 ggggttgtgt atactagacc agggcgcgga catgactttg ttccagagtt acgatttgaa
     1681 gattttttag ataaacagct atctatagat gaaacagcca gttactatca taggggagta
     1741 tgtatagagg gagcggattc atttgaaaat atactagatt tcattgattg gctacctaag
     1801 attgggatga acagtttttt catccagttt gaaaatcctt actctttttt gaaacgttgg
     1861 tatgaacatg aatttaatcc atatctaaat aaagaacaat tttcaaatga attagtacaa
     1921 gaattgagtg ataggttgga taaagaattg caaaaaagag gtcttattca tcatcgtgtt
     1981 ggtcatggat ggacaggtga agttttaggt tactcttcaa aatttggctg ggaatcaggt
     2041 cttagtattt cagaggagaa gaaaccctat gtcgctgaaa taaacgggaa acgagaattg
     2101 tttaatacgg ctccgatttt aaccagcctg gatttttcaa atccagatgt agctgataag
     2161 atggtagaaa ttatcaagga ttatgccaag aaaagacctg atgttaacta cttacatgta
     2221 tggttgtcgg atgctcgtaa taatatttgt gaatgcgaaa actgtagaca agaattggtt
     2281 tcggatcagt atattcgtat tctcaatcaa ttggataggg ctttaacgag tgagggatta
     2341 gatacaaaga tttgttttct gctttatcat gagttgttat gggcacctca gaaagaaaaa
     2401 ttagataatc ctgaacgctt taccatgatg tttgcaccga ttacaagaac atttgaaatg
     2461 agttatgcag atgtagattt tgataattcc atacctacgc ctaaacctta tatgcgtaat
     2521 aaaattatac ttccgaattc tcttgaggaa aatttatctt atctttttga gtggcaaaaa
     2581 gcatttaaag gagatagttt cgtatatgac tatcctttag ggcgtgctca ttatggcgat
     2641 ttaggctata tgaaaattag tcaaactatt tacagagatg tatcttatct ttccaaccta
     2701 catttgaacg ggtacatttc gtgtcaagaa ttacgtgccg gattccctca taattttcct
     2761 aattatgtca tggggcaaat gctctggaag aagacaagaa gttatgaaga attgattgaa
     2821 gaatactttt ctgctttgta tggggaaaat tggcagtctg ttgttgaata tttagaaaaa
     2881 ttatccattt attcctcttg tgattatttt aatgcaattg gcagtcgtca aaatgatgtt
     2941 ttagcgaatc attattatat agcttacaat ctagctgata attttttacc aattattgag
     3001 gaaaatattt ctaagttatt aaatagtcaa aaggatgaat ggaaacagct cagttatcat
     3061 cgtgaatatg ttgttaagat ggcgaaggct ttatatcttc aagcaactgg aaaaacaagg
     3121 caagctcaag atgaatggag aaatgtgttg aattatatcc gtgggcacga attgctattt
     3181 caatctaatt tggatgttta tcgtgtaatt gaagtagcaa aaaattacgc tggtttccac
     3241 ttataaatca taagtataga aaatgaacta aggtattcag agaagattga ccctaaatat
     3301 tatgaaattt aaggattttt aagatattta gggtcgactt tctatttgta tcgtagcgaa
     3361 gtcattttaa taatgatgtg taaaagatgg atcaagattg aggaggaaga aagatgaaat
     3421 caaaagaaga aataaatatg cttggtttta caattgtcgc ttacgcagga gatgcaaggt
     3481 cagatttgat ggatgctttg gcgtttgcga gagatggata ttttgaacag gcaagagaat
     3541 tggttgagtc tgcaaacgac tcaatagtgt ctgcccatcg agaacagact aatttattag
     3601 cggaggaggc atatggagat aattttgaag tgagctttat tatgattcat ggtcaagata
     3661 ctttgatgac aacgatgcta ttgtatgatc aggtaaagtt ttttattgat gaatatgaac
     3721 gaattcgaaa gattgaagaa catattggtt tgcaatgagg attagtcatg gaaaatttac
     3781 agtttaaagt cttaccgaag gagtttttat taggagctgc taccgctgct tatcaagtag
     3841 agggtgcaac tagggtagat ggcaaaggaa taaatatgtg ggatgtttat ttgcaagaaa
     3901 atagtccgtt cttaccagat ccagctagtg atttttatta tcgttacgaa gaggatatag
     3961 ctttggcggc agaacatggt ttgcaggctt tgcgtttatc tatttcttgg gttcgtatat
     4021 ttcctgatat agatggagat gctaatgtat tagctgttca ttattaccat agagtttttc
     4081 agtcttgctt aaaacataat gtgattccgt ttgtttcttt acatcatttt gattcgcctc
     4141 agaaaatgtt agaaacaggg gattggttga atagagagaa tattgatcgt ttcatacgat
     4201 atgctcgctt ttgtttccaa gaatttacag aagtcaagca ttggtttaca atcaatgaac
     4261 tgatgtctct tgctgcaggt caatatatag gaggtcagtt tcctccaaat catcattttc
     4321 aattatctga agcaattcaa gcgaatcata atatgttgtt ggcgcatgct cttgcagtcc
     4381 tcgaatttca tcaattaggg attgagggaa aggtaggttg tattcatgct ttaaagctag
     4441 gctatcctat tgatgggcaa aaagaaaata ttttggcagc taaacggtat gatgtttata
     4501 ataataaatt tctattagat ggaacttttt tgggctacta cagtgaggac acgctttttc
     4561 acttgaatca aatattggaa gctaataatt ctagctttat tattgaagat ggtgatttag
     4621 aaattatgaa gagagctgca gctcttaata cgatgtttgg gatgaattat tatcgttcag
     4681 aatttattcg tgaatacaaa ggtgaaaata gacaagaatt taattcaaca ggaataaaag
     4741 gacagtcttc ttttaaatta aatgctctag gtgaatttgt aaaaaaacct ggtattccga
     4801 caacagattg ggattggaat atttatcctc aagggttatt tgatatgttg cttcgtatca
     4861 aagaagaata tcctcaacat ccggtcattt atttaactga aaatggtaca gcccttaaag
     4921 aagttaagcc agagggcgag aatgatatta ttgatgacag taagagaatc cgttatattg
     4981 agcaacattt acacaaagtt ttagaggctc gagatagggg agtcaatatt caaggctatt
     5041 ttatatggtc tttgcaagat caattttctt gggcgaatgg ctacaataag cgatatggtc
     5101 ttttctttgt tgattatgaa acacagaaga gatatattaa gaaaagtgct ctttgggtaa
     5161 aagggctaaa acggaattaa ggttagcgat ttgactgatg tttaatatat tttaactatg
     5221 agcttaaatt ttttataaga ggagttttat ggataagcta gtcgctgcca ttgaaaagca
     5281 acaagggaaa ttt
//
LOCUS       DAVHIU010000112         5276 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVHIU010000112 DAVHIU010000000
VERSION     DAVHIU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.114"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..849)
                     /locus_tag="VVN06_001748"
     CDS             complement(<1..849)
                     /locus_tag="VVN06_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5563242.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLMSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYD"
     gene            complement(850..1419)
                     /locus_tag="VVN06_001749"
     CDS             complement(850..1419)
                     /locus_tag="VVN06_001749"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5563243.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDEYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(1490..2605)
                     /gene="ychF"
                     /locus_tag="VVN06_001750"
     CDS             complement(1490..2605)
                     /gene="ychF"
                     /locus_tag="VVN06_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5563244.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(2725..3891)
                     /locus_tag="VVN06_001751"
     CDS             complement(2725..3891)
                     /locus_tag="VVN06_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5563245.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(4327..4887)
                     /locus_tag="VVN06_001752"
     CDS             complement(4327..4887)
                     /locus_tag="VVN06_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter"
                     /protein_id="HEU5563246.1"
                     /translation="MELQQYDELTFENIKHIDENGVEFWYARDLQKTLEYTEWRNFLL
                     VIDKAKIACDTAKNPIVEHFVDVNKSIPNGYNTTRDIKDFKLTRYACYLIVQNGDPRK
                     EVIALGQTYFALKTRQQELQENFDNLTEEQKRLAIRDEIKHHNKSLSESAESAENATY
                     SLPRATMFNNPEARAFSGFSKSSDLV"
     gene            complement(4992..>5276)
                     /locus_tag="VVN06_001753"
     CDS             complement(4992..>5276)
                     /locus_tag="VVN06_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEU5563247.1"
                     /translation="DKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRETLIERVE
                     TCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTILVRLN"
BASE COUNT         1500 a         1037 c          910 g         1829 t
ORIGIN      
        1 atcatagcac aaagataaaa acttccgaga gtctgcatga ctttgttttt ggtgaaaact
       61 tgaaagaatt tcttctaggt atgatttcaa aataggtgat aaagtttttg aaatttgttt
      121 ttctaaagct gactgtcctc gttgataatc cttttctctc aaaagcatat cactagctgg
      181 aaagatagtg agttctgtct tattttcttt cgataattgt gtttctactt caaatgacct
      241 gataccatca atttcatcac caaaaaactc aattcgacaa ggttctaact gggatatttc
      301 aaaaatatct aaaatatctc ctcgaagact aaattcgccc tgagtttgta cttgagtaac
      361 ttttcgatag ccattttcct ttaactgatg gataaacgcg tgttgatcat attcttcacc
      421 aactgagatt tttacaatac tatctttgaa tgcattggga gacggtaaaa tcaatcgact
      481 tgctgcgata ttacaaacta aaatcccttt cttagatgaa tcagtcaaaa aacgcaaggc
      541 ttcaacccgt gaaataattt tttcctgtga agacatcaaa aactccacca taggagcatc
      601 atctaccaaa aatggataga cgagttcctc acccaagata gatataagat cactaacaag
      661 tccttctgct tctccataag ttgacatcaa taacacaatc ctatcttctt tttctaaact
      721 gcttgcaatt gcaagagcct tagtagatgt tgataaacca agtattagtt gtcttttctt
      781 atctgttaaa ttttgatgcc attttttaat ctgatcattt tctgagaata aatctaataa
      841 ggtcaccatt tatccgttat acctctgcat tgttttctca aaatttttct cttgtaaata
      901 gtagtttaca gaatcgtcaa ctttgtcaac agactgtaaa ataccgatat actcatccct
      961 gtcaaactta ctcaaaacat gatgaacaac tgacatacca tttttaggtc ttccaattcc
     1021 aatcttaaca cggttaaaga cctgagttcc tatatgttga ataatagact tgataccatt
     1081 atgaccacct gctgagcctt ttgctcttaa acgaattttc ccaacttcca tgtcaagatc
     1141 atcgtaaatg ataagtaaat cgtcaatatc caaaccatag taagttaata aagcatgaac
     1201 tgcttttcca ctttcattca taaaggtcgt tggtttaacc agataaattt tttctccatt
     1261 taggaaaaag gatgctaggt cagcttgaaa tatcttatcg tgtgtaaaag tgacattctg
     1321 tttcttcgct agttgatcaa tcaacataaa accaacattg tgttttgttt caaaatattt
     1381 atcccctgga tttcccaagc ctacaagtaa tttggtcatt tatttttcct ttcaaaagcc
     1441 aaaagggttg gaattttttt ccaacctaat tgacaccatt tattaatttt tagacattaa
     1501 agcggaattc catgatatcg ccatcttgaa cgatatattc ttttccttct tcacgcaagc
     1561 gtccagcttc ttttacggcc ttttcagatc cgtatttcac tagatcttca tatgacatgg
     1621 ttactgcacg aataaagcct ttttcaaagt ctgagtggat aataccagct gcttgaggag
     1681 ccttcatacc acgtttgaaa gtccaagcgc gaacttcttt ttcaccagct gtgaagtaag
     1741 ttcccaatcc aagcaagtgg taagctgcac gcgtcaactt atctacacct gattctgtca
     1801 aaccaatggc ttcaagaaac tcttttttat cttcatcatc caattcagaa atttcttcct
     1861 cagcacgcgc agaaataacg actacttcag cattttctgt cgctgcaaat tcacgaattt
     1921 gtttgacata gtcgatagag tcaggttctg aaaccacatc ctcgtccaca ttagctacat
     1981 aaagaactgg tttagtcgtc aaaaggaaaa gacctttgac aaccttttgt tcctcatctg
     2041 taaattcaat ggtacgagct gatttcccgt cttctaggac tggtttaatc ttttgaagaa
     2101 cattgaattc tgctactgat tctttatctt tttgcgtacg tgccatcttt tctacacgcg
     2161 catatcgttt gttcactgat tctaagtcag caagaattaa ttccagatta attgtatcaa
     2221 tatctgcaag tggatctaca aaggcgtctt cacgtccttg ctcgcgcatc acattttcat
     2281 catcaaaagc acgaactacg tgaacaatcg catctacttc acgaatattg gccaagaatt
     2341 tattccctag cccttctcct tttgaagctc cttttacaat ccctgcaata tccgtaaatt
     2401 caaatgttgt gggaactgtc tttttaggag ttatcatttc agttagtttt tgtaggcgtt
     2461 catctggaac ttccaccatt ccaacatttg gatcaatagt cgcaaatggg tagtttgctg
     2521 cctctgctcc tgcttttgta attgcattaa atagtgttga tttaccaacg tttggcaaac
     2581 caacgatacc tgctgtcaaa gccatatttc tcattctccg ttttcatttc aatccctaat
     2641 attataacac aaaaagggta aacttgctaa aattccttga tatagctatt tcccataact
     2701 gaaataataa ttttatggac ttttttagga actttttaat ttttcaatag ctttttcata
     2761 aaatgaagta gcatttttct gattgtcttt ggataggtgg ctataaatat ccatggtcat
     2821 agaaagtttt gcatgaccga gccgtgtttg tatctctttg taaggtagtc cagcattgag
     2881 caagatacta gcatgagtgt ggcggaaggc gtggaacgta aaacgagggc atccagcacg
     2941 ttccaaatgt tttactaacc tgaaactaag cattctagcg tctatatgct tatcaaagct
     3001 attagagaat actttctcat aggtcaaccc catttctttc cctatttggg attgtcttgc
     3061 tttatagagc cgtagcatga gcaccgtctt attatccaag tcaattaccc taatacttga
     3121 tttagtctta gggccagtga tttctttgat aatatttaac gttttattta cgtctagtgt
     3181 gccgtttttt aggtcaatat cagaccattt gagggctaaa cactcacgaa tacgcaaacc
     3241 agtcgctaaa agcgtcttat atagcaccgt atcgtagaaa tttttgtaag tgtttggcag
     3301 ctgctccagg taagtcaaga atttttttaa gttatcatca tccagatatt tcagtttttg
     3361 cccttctttt tccttacgac gtggcacgat aacatcacgg gcagggttaa aggggatagc
     3421 ttgcaaagat acggcatatt taagtatgcg tttgtttagc gagtgaagga gaggatactc
     3481 ttgaaaccct ttgcctagtt gattatagtc ttttgcccat tgattgactt gcttttgaat
     3541 gattgggggc gtcagtttat ccagcctata atttccgaaa gctggaagta gatatttatc
     3601 tattttgaat ttggtaccca ttaaggtcga ttttttaacc gtatggcagt acgtttctag
     3661 ccaattctcc accagttcgg cgtaagtctt tatctcaact gctttatata ctgtagaccc
     3721 gtctttttca aagtcgattt gagcctgtag cgctttggct ttgagttctc tcttggttcg
     3781 ccctgatata gtcgtcttga ctttcttacc tgttacggta tcgataccaa ggtagacgct
     3841 agagcgatag actgtagacc cgtctttttt ggtgtattct gttattttca tggttttact
     3901 cctttttcca tcagcaggca agcaattaga aaaggttttg agtttatacc atgcgagggg
     3961 ctacgagaat acccctattt tcgattttaa ttagccaggc ggtaaataat accagaatag
     4021 gaaataaggc ggatatgggg tttgtatgag cgtttttaca tatcatcaaa gagtgaaagt
     4081 tggttattat ccggttgctt tttaagtttc ttttgtttgt tttttaggtc ttggcaacca
     4141 cgcttttttc ggagtcttca cataaatgtt ggaaaaagtc ggattttcaa gaatactgga
     4201 aaaaaacaat tttcttcatt ttctgagtcc ctaaaaagcg atggggtaaa caattccaat
     4261 cccgtttcta ttagttctta atggttggaa aactaacatt tctaacaccc taaaaaggag
     4321 aagggtttag acaaggtcag aggacttgct gaagcccgag aaagcacgcg cttcaggatt
     4381 gttgaacatt gtggcgcgtg gtaggctata ggtcgcattt tcggcacttt cggcagactc
     4441 agataatgat ttattgtgat gtttaatttc gtcacggata gcaaggcgtt tctgttcttc
     4501 ggtaagattg tcaaagtttt cttgtagctc ttgttgacga gttttcaacg cgaaataggt
     4561 ttggccaagt gctataactt cttttcgtgg gtcaccattt tggacaataa ggtaacaagc
     4621 gtaacgggta agtttaaaat ctttgatatc tcttgttgtg ttataaccgt ttggaattga
     4681 cttgttgacg tcaacaaaat gctctacaat ggggttttta gcggtatcgc aagctatctt
     4741 tgccttatca attactagaa gaaagtttct ccattctgta tactcaaggg tcttttgtaa
     4801 gtcccgagcg taccaaaatt ctactccatt ttcgtctatg tgtttgatat tttcaaaagt
     4861 taattcatcg tactgttgga gttccataat aaagtatcct ttctaaaata ttgtaagtgg
     4921 ttgacatttg ttgacatcaa caaaactagt agatatggga cttatatggg cttgtttgtg
     4981 atgggtgatt tctagttcaa tctgactaag attgtttgat aagaatattt cattgaccgt
     5041 ctccaattac acataggaca caatttattc ttacaaaaat aggcttgata taaacgcaaa
     5101 ctcccatctt ctcgacgaat aaatcgaaga gtatctccac aagtctcaac gcgttctatc
     5161 aaggtttccc tataacctaa ctctttaaaa atatctgcca atttcaaact taataatttt
     5221 cgtcctcgcc agtttctatc cttgcctctt ttatttttat ctaaaagaat tttatc
//
LOCUS       DAVHIU010000113         5252 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVHIU010000113 DAVHIU010000000
VERSION     DAVHIU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.115"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..186
                     /locus_tag="VVN06_001754"
     CDS             <1..186
                     /locus_tag="VVN06_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5563248.1"
                     /translation="EDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSM
                     LSHLKKEVETHYQPKKL"
     gene            complement(476..946)
                     /locus_tag="VVN06_001755"
     CDS             complement(476..946)
                     /locus_tag="VVN06_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5563249.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(912..1718)
                     /locus_tag="VVN06_001756"
     CDS             complement(912..1718)
                     /locus_tag="VVN06_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5563250.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(1925..2566)
                     /locus_tag="VVN06_001757"
     CDS             complement(1925..2566)
                     /locus_tag="VVN06_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563251.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(2563..4671)
                     /locus_tag="VVN06_001758"
     CDS             complement(2563..4671)
                     /locus_tag="VVN06_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5563252.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(4726..5019)
                     /locus_tag="VVN06_001759"
     CDS             complement(4726..5019)
                     /locus_tag="VVN06_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU5563253.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
BASE COUNT         1630 a         1162 c          850 g         1610 t
ORIGIN      
        1 gaggacgcca agaataagaa aagctacgat aggcttgcta tctgctatgt tcgtattggg
       61 atttgtaggg ataatgctaa acttatccaa aaaggtttct cactcctaga gctgaccgag
      121 gaaacttcta tgctgtctca tctcaaaaaa gaagtagaga cccattatca accaaagaaa
      181 ttataaaaaa agtcgaggga tttcctcgac tctttacata ttatttgttc gttagttctt
      241 aataccagcc attgttaaac cagaagtttt tagctctgcc aagccacata gtgtgtttac
      301 tactatgtgt ccatgtcacc atgaatcgcc gaacgattta acgagaaagt atgactttta
      361 cgtttatccc aactcaatta tgacattttt ttcaaaagtc aatatatctc actttttcaa
      421 cgacaagaaa gaggctgcca attgaccaac ctcttattct gaacccatca ctccatcact
      481 ttttagcttc attcgctttc ttagcgactg caatctggta ttcgacttgg tcattcccct
      541 tacctgtaca accatgagca attgtagtcg ctcctgtctg atgcgctatt tcaaccaatt
      601 ttttagaaat aagagggcgg ctcaaggcag ataccaaggg gtacttttgt tcatagtagg
      661 catgtgactg gagagctact agcacataat ctgtagcaaa ttcgtcctta acatcaatga
      721 cataagattc gactgcccca accttaagag ccttatcatg gatgaaatct aagtctttcc
      781 cttcacccac atccatacaa actgcaacaa catcatagtc tttcttcagc caggtaatag
      841 caactgatgt gtctaaaccg ccagaatagg ccaaaattac tttttcttta ctcatgttct
      901 tttccttctt tctatttttc aatggacgct ttaaaggcat cctcaatgag tttgtctaat
      961 tccccagact ccttcaactt ttgaatggtt ttatcgactg cctctttcaa ttccttgcta
     1021 tcttttttca tggctaccgc ataggaatca tcttgctctt tttcaaaatt gaggtctgcg
     1081 attgctaaat caggattatt ttccacaaat cccttggcaa cgggttcttc aaaaataacg
     1141 gcatccactt gccctgattt taaatctgtg attaaattcc catttttagg cagagatacg
     1201 agggaagaat tttgtagcaa atctttcgcc atcgtctctt gaatcgaacc tttctgcgct
     1261 ccgacctttt tctgcgccaa atcgttgaca gactgataag tggctaaatc agattttttg
     1321 acaatgagtt tattttttgc agtatagtag ggagttgaaa aatcaaacac cttgctccgt
     1381 tcatctgtct tagaaacacc tgatatggca aggtcagctt ttcctgattg aacgctagcc
     1441 agtacattgt caaaactcat aggagatagt tccaattcga cacctagttc tgttgcgata
     1501 gccttggcta attcgatatc tgaacccaca atctgatttt tcccatcaac cactttttga
     1561 tattcaaatg gagcaaaatc tggattgagg gctacaacca attttccttt ggacttgatg
     1621 gcttcaattc cagccgattg attgttacta caagcaaata gtagggggag catcactaaa
     1681 ccaaacatcg tcatcaacac cttcttcatc ttattcataa taaagcctcc tttatgttta
     1741 ttctttttta ttgtataaat aatactccta tactcatcct ttgtcaagcc taaattgaaa
     1801 aaattttcaa tttgaaacaa aaaaaaccta gaaatactac ataaaatgtc attcctaggc
     1861 gtatttatgc tatttttctc tggaaaacat gagtattcag ccagtcaaaa ggagctgaat
     1921 gaactcattt tccctcgccc aattcaatga cacgatgaca ttgttgggct acataagcat
     1981 cgtgggtcac aataatgact gttttccctt ctcgattcat ctctaagaga aacttcaaga
     2041 ccaaatctct attttcagga tccagcgaac ctgtaggttc atcggctaaa atcagctggc
     2101 tgggttttaa gatggctcta gcaactgcaa ttcgttgttg ttcgccccca gacaactcgg
     2161 agaccctttg atgcaaagta gctgacaaac ctactctctc taaaatctct tccacctttt
     2221 tgagcttgtc tttcttaggc aatttcacat atttcagcgc cagcatgaga ttgtactcga
     2281 ccgtttcatc atcaatcagg gcaaaatttt gaaacagata agagatatgt tcacggatta
     2341 ttgtttgcga cttagcagaa ttaaccgcta gatttgtctg accaaaaatc tcataccgtc
     2401 cgctataatc accatctatc aaacccaata aatttaacaa ggtcgacttc ccactaccac
     2461 tcttaccaac aatagctacc aaatccccct gatcaatcct gagagataag ttatccaaaa
     2521 tcacttttcc cccaatggtt ttggtaatat ttttcaactc aatcataaga tgcccccttt
     2581 caataactct actagacttc ttttctccat cctagaagct aagcctagca caaatagtat
     2641 atccagacat gtaaaacctg caaacagtag aagtggtaag aacgcatggg caaagaaaat
     2701 caagactaga agagggaaac tatagcccag caagagcaga acgaggagag gacggtagcg
     2761 atcgaccagt ttccacccca taaacttctt ggtaatgata tccctgcgct tcaataagaa
     2821 agttgttact agtaagaagt aggaaatcat catgctaagg agaccaaaca aagcaaagag
     2881 taggttaaaa ttccgaacag catctcgata agaatccact ttctcttgtt gaatggcttg
     2941 aatagatgaa aattttaaat aatttccatc tgacaatttc tcaactaact ctgtaatctc
     3001 tttttgatgt tgaaccgtat tttcaatttt aatcggatta tttaagccag ttgttgacag
     3061 ggaggctttc tcatcccaca tcatatcaga atcattgacc aagctaataa ttggattgga
     3121 gagattttcc tttcgcttat cactatatgg gaaaaatgac caatctcctt cataataggc
     3181 aatctcgaca tccatctcct ctatcgttcg tttttgctgc tcttcatact tcatcgaatg
     3241 aaaggcaatt aacttcccca agagctgatt tttatcttct tcacctttcg tacttgctgg
     3301 catcaaaata acttttttaa taccggtatc tggtagcttg aatcccttgc tctttagaaa
     3361 attgcgattg gcatagtaaa catccaccgt atctgttaac tgatattgct gaatctgttc
     3421 tgattggaca aaatttttta caggaagact gctactctgc acatagcccg cctgcgtttt
     3481 ttctaccaaa tcctgataaa atcgatagaa ataatccaat gccttccctg agcctgccaa
     3541 ctgctcttgc cacaagttat cattgagttg gaaagtttct aaggtcagat aatttccttg
     3601 gctgatccac tgtttctggt aatccaactc tttgttttct tgctccaaac tttttcctac
     3661 cccaactagc aagactgtca gtaaaatggt agttccaatt ttcatcaaat aattaaagag
     3721 tagaccaaat ttgaaagatg aaaaaccttt cagcagagag ctgattgtca ttttttggat
     3781 taagaggtaa gtcaaccaac tgataaagag ataaagctgc aacagcaaaa aatgagacaa
     3841 ccacagcata ggaaacaaat cttttggctt ataatcaagc aagaaaaaca cgcctagatt
     3901 gatcacaaga gccccaccta ggaggaggta aaggttgcct tttacaacat cagctaaaac
     3961 agccctatct tgaaaaccaa gtaatttttg taccccaact cgtttcatct ccatcatcgg
     4021 ttgatacact gtcactaaca caagaagcaa aatagccaag acaaaaacaa tggcagataa
     4081 aagcaaatct cgatttatga cttccactgc acttttgtag gtcggctcta gcaaggtagc
     4141 ctggtctatc ttgaaaaaat cgctccattt ctgtacaatc ctatccttgt ccatctcttg
     4201 tgtagaagtt atcgtatagc gaccatttaa actacgagat gtatccttga tataggtttg
     4261 aaaagtcata agctgaatag gtttggcttt tagaaaggtc ggaatcgtac caagtttatt
     4321 ggaaatttct ttattactat agactccttc accatctgtg gtaaaatcaa gagaagaaat
     4381 cccaaactct tggtagggga aggtatcttt atcaaaaaca ccagacttga ccacctcatc
     4441 accactgtct gttttgatga tggagacttt atactccttt gatacatcct caaaaaatcg
     4501 aagaacagac gctgcaggtt cgttaatatc tttcaaatac aaatccaaag aatctacagt
     4561 ctttcccatt tcttgaatcc gaaccacttc tcgtgtatag tttacattaa aaacaaaaaa
     4621 agtggtacac agaagcagga gtaacataca aagatgactg atttttttca tagagatacc
     4681 tcccgtgaag aaactccctc tccaaaaggg gagggagaat tttaattaaa atccatagaa
     4741 atattccatt cccgttggtg ggaatttgaa gattgaagcc cgagcccatg cctcagcttc
     4801 tgcacgatcc ttagagaatt tatttccatc tttaacagtt gctgtatgag agcgagtaga
     4861 atgtaaataa tcagaataac caaaatttcc tgaccatcct actccatagt tccattgacc
     4921 accatcaacc catactgcca aaacacttgt agaagcaagt atactacata ccattaaaga
     4981 tgcaacacca attttctcat gatttatttc tcctattatc gagagctatt ccgtagaaac
     5041 agctatagta atttgtcaac aagtatcgta gtccattgga ctcaccttcc ttctatctca
     5101 tttcattttt aaacaacgaa cttactctta actagaagaa tagttctctt ctgtatcagg
     5161 tctttgaaca accagagtag tattaatcac actctaacta ttactcaaga atcagcccaa
     5221 taaacaatat ggatagcgct ttcaaatttt ta
//
LOCUS       DAVHIU010000114         4980 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVHIU010000114 DAVHIU010000000
VERSION     DAVHIU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.116"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..184)
                     /locus_tag="VVN06_001760"
     CDS             complement(<1..184)
                     /locus_tag="VVN06_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563254.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKS"
     gene            complement(644..1372)
                     /locus_tag="VVN06_001761"
     CDS             complement(644..1372)
                     /locus_tag="VVN06_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5563255.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1530..2939
                     /locus_tag="VVN06_001762"
     CDS             1530..2939
                     /locus_tag="VVN06_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU5563256.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2957..4252
                     /locus_tag="VVN06_001763"
     CDS             2957..4252
                     /locus_tag="VVN06_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5563257.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4257..4565
                     /locus_tag="VVN06_001764"
     CDS             4257..4565
                     /locus_tag="VVN06_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5563258.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4562..4870
                     /locus_tag="VVN06_001765"
     CDS             4562..4870
                     /locus_tag="VVN06_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU5563259.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1698 a          849 c          893 g         1540 t
ORIGIN      
        1 ttgactttcc tgtaccattt ttaccagtaa ttaatgtcac gcctgaactc gaagtatcaa
       61 aagaaatatt atcaaacaat atacgatgat ccaccctata cgaaatattt tctactttca
      121 ttttttctcc ttttgaacta atagcaaaag aaacagttct gttttcatac tctttcccct
      181 tcatctatat tattcgtaat gaaaaataaa aaaataaaat ttttttaaat gattatgatt
      241 cagaatttat tcaatatatt aaaacaatat ttgaacaaat aaattgggag attgaaattc
      301 atttctagca atttgttaga agtcacagtc tactattcta acctcagtat actatatgtg
      361 ttacaagaaa agactcaaaa acttctcgtc agtgtaagaa tatttgaaac actatttaaa
      421 actaaataac tataagaagt agatcaggtg aacaatttca gtacctactc tataagctat
      481 acgaagatat ttttcaacat gatggataaa acgatcactc ttttaaagcc atcttattta
      541 gaaaagagaa accatttaag catagatggg accttcttgc cctgttttaa atttttctta
      601 aactagagtc tgtaaaagat tggcttcatt ttcacatttt ctcctaggag gaatgtcgta
      661 atgcaaaaga attaaattca aataaatcat agcgatgacg actaatcgag tactcaaacg
      721 gagttccgtt atccaaataa gccacttgtt ctacttcaaa taccggttcc gttggaagga
      781 gctgcaagta atgttgctca ttttctgaac tagaagaagc tcgtaagatt tttgtagcac
      841 tggcaatatg caatcctaac tttccttcaa tgtaattgta aatcgatttt tgtaaaatat
      901 cttcagtaat acctggaata acatcggtac tcatataagt ttgttccaga acataaggtt
      961 taccgtcaat aatacgcagg cggtaaatat tataaactgg actctgcaaa gcgacctgta
     1021 gtttttctgc taaaaattca tttgcaaatt ctaatttgaa gtgaataatc ctcgagtcaa
     1081 ttgtagaatg agcatcttca gatatttttg tcagtccccg gatatctctt tctggaacga
     1141 ttaattttct ttttgagctg ccacgagaga gaacaaaggt tccctgccca cgttttctaa
     1201 aaattaagcc ctcagaaact aacaagtcta aagctttttt gatcgtcatt cgactacagg
     1261 caaactgttt agctaatgtc atttcatcag gtaattgttc gttcatggta tatttattac
     1321 aaagaatctt ttcacgaatc tcattgtaaa taaataaata ttttgtactc atttaatctg
     1381 gttctctcta taatattcta atagtagtat aacaaagttc aagaaatgag gcaaacacaa
     1441 gatatgtatc aacaattttt aaaaatgtct agacaaattt taaaattatg ctatacttta
     1501 tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa gttagtattt cctaatctgt
     1561 tttggtgggg agcagcttct agcggacctc agacagaagg tcaatatgga aaagtacatg
     1621 aaaatgtgat ggactactgg ttcaaaacgc atccagaaga ttttttcgat aatgtcggac
     1681 ctcttgtagc cagtaacttt tttcatactt acaccgaaga tttccacttg atgaaggaaa
     1741 ttggagttaa ttctttccgc acttccatcc aatggagtcg actcatcaag aatttagaga
     1801 caggtgagcc tgatccaaaa ggtattgctt tctacaatgc catcattgaa gaagctaaaa
     1861 agaaccagat ggatcttgtg atgaatttac atcattttga tttaccagtg gaacttcttc
     1921 aaaaatacgg tggttgggaa agcaaacatg tagtggagtt attcgtgaag tttgccaaga
     1981 ctgctttcac atgctttgga gataaggttc attactggac aactttcaat gagccaatgg
     2041 tcattccaga agcaggatac ttatatgctt tccattatcc aaatctaaaa ggaaagggaa
     2101 aagaggccgt acaagtcatc tataatctaa accttgctag tgcaaaagtg attcaactat
     2161 atcgctcatt agaacttgat ggaaagattg ggattatttt aaacttgaca cctgcttatc
     2221 caagaagtaa ttctccagaa gacttagaag caagtcgatt tacagatgac ttctttaaca
     2281 aagtcttctt gaatccagct gttaaaggaa ctttcccaga aagattggta aaacagctag
     2341 agagagatgg cgtgttatgg agtcataccg aaaaagagct tcaactgatg aaatcaaata
     2401 cggttgattt tcttggagta aactactacc atccaaaacg tgttcaagca caagcaaatc
     2461 ctgaggaata tcagacgccc tggatgccag accaatactt caaagagtat gaatggccgg
     2521 agcgtcgcat gaatccatat cgtggttggg aaatttttcc gaaatccatt tatgatattg
     2581 ctatgattgt gaaggaagaa tatggtaata tcccatggtt tatcagtgaa aacggaatgg
     2641 gtgttgaaaa cgaagcacgg tttatcgatg aaaatggagt tatcgatgac gtgtatcgta
     2701 ttgaatttta tgaagaacat ttaagatggc tacataaagc cattgaagag ggaagtcact
     2761 gttttggata ccacgcttgg accgcatttg attgctggtc ttggaataat gcatataaga
     2821 atcgttacgg atttatctcc gttgatttag aaacgcaaaa gagaaccatc aagagctcag
     2881 gaagatggta tcgcaaagta agtgacaata acggttttga agtagaaatt gaggagtaaa
     2941 gagaggagat attcatatga aaaactcaaa atttatagac caatttgcca cctttgctgg
     3001 taaactaggg aaccaaattc atttaaaaac cctaagagat gcattcgtaa cagtaatgcc
     3061 attatatatt ttggcaggtt taatcgttct tttgaacaac acggtattta agtggatttt
     3121 ccaaggggat acattaacaa gattccaata ttggggaata acaattgcaa acggtacttt
     3181 aagtatttca ggtatgatta ttgctgtaat ggttggttat ttcttagcta aaaacagaga
     3241 tttcgaaaac ccgttagcag catcaatgct atcattagtt tctttaattg tgatgatgcc
     3301 aaatacagtt tctgtagttc ctgacggagc aaaagatgct gtaaacattt caggtgttct
     3361 ttcattcaac aacacaggta caggcgcaat gttcgccggg gttatcgtag cgattattgc
     3421 aacagaatta ttcattgaat tatcaaatgt taaagcttta caaatgaacc ttggtgaaaa
     3481 tattccacca gctgttagta gatcatttag cgtattactt ccagtcatga ccgtcatctc
     3541 cttatttggg gttgtttcag cattattatt caatataact ggaatgaact taatctcaat
     3601 cattacaatc tttattcaag aaccaattcg tcatattggt acaagcttaa tcggggtcat
     3661 tattatttac tctttaggaa atatgttatg gctatttggt attcaccaag cagttattta
     3721 cagtgccatc ctagaaccat tactattaat taacattact gaaaacatca ctgcagcaaa
     3781 taatggacaa gccattccac acatcatcaa cctatcacaa atacaaacat tcgctttaat
     3841 gggtggtagt ggatctacat tatgtttatt aatagcaaca ttcttagtga gtcgcaatgc
     3901 tgtctctaaa aacgtggcta aattatcttt tggacctggt atcttcaata tcaatgaacc
     3961 agtattattc ggttacccaa tcgtttataa catttcatta gctattccat ttatcacagt
     4021 tccagtcctt ggtattttaa tcagctactt agcaacagtt acaggattca tgagtcctgc
     4081 atttatacaa gttccttgga ctacaccagt attcttaaat gcatggttag caacagcagg
     4141 ggacgtgaga gcagttctag ttcaattcat catctttgca cttggagttc ttctatacat
     4201 tccatttatc aaagttaatg acaaagttgt tgaacaagaa atggaaggtt aagattatga
     4261 aacaaatttt attagtatgt aatgcgggaa tgtcgacaag tatgcttgtt aaaaaaatgc
     4321 aacaaagtgc gactgaacgt gggattgaaa tctctatcca agcaaaatca atgacagaag
     4381 ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg gccacaaatt cgttatgagt
     4441 tacttgctgt gaaagaaatt gcaggaaata tccctgtaga taccattgat atgcgggatt
     4501 acggaatgat gaatggagca aaagttctgg aacaagcact tgcatggata ggagaaataa
     4561 gatgacagaa gaaatggaat atatctgttt tcagctcatt gccaatagtg gagcagccaa
     4621 atcatccttt attgaagcca tccaactagc taaggcagga aatttaaaag aagcgaaaat
     4681 taaagttgaa gaagcggaag actccttagt agaagcgcac aagattcact ctaatctcat
     4741 acaaaaagaa gctactggag aaaaaatagg attctccttg ctatttatgc atgcagaaga
     4801 ccaaatggct tcaacagaga ttattcagtt gctatctaaa gaattcatcg atctctatca
     4861 aaataaatag gaacactgtc attcccatct gtctttatta atgacgacta cattgtagga
     4921 acctccaaaa gttggacaaa atgattgaaa ggattggatc ctgcattaca caagatccaa
//
LOCUS       DAVHIU010000115         4904 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVHIU010000115 DAVHIU010000000
VERSION     DAVHIU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.117"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..558
                     /locus_tag="VVN06_001766"
     CDS             <1..558
                     /locus_tag="VVN06_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5563260.1"
                     /translation="TRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNW
                     GKFIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLD
                     VRPYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYG
                     LSPLKSINFHRARWKEMVKSYFEKK"
     gene            650..1504
                     /locus_tag="VVN06_001767"
     CDS             650..1504
                     /locus_tag="VVN06_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5563261.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMCISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            1616..2500
                     /locus_tag="VVN06_001768"
     CDS             1616..2500
                     /locus_tag="VVN06_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU5563262.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            2663..4402
                     /locus_tag="VVN06_001769"
                     /pseudo
     CDS             2663..4402
                     /locus_tag="VVN06_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            4677..>4904
                     /locus_tag="VVN06_001770"
     CDS             4677..>4904
                     /locus_tag="VVN06_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563263.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNF"
BASE COUNT         1596 a          759 c         1053 g         1496 t
ORIGIN      
        1 actagagcat tactaaaaat gatgggctta aacgttccgg tttatgacta tcctattgag
       61 cttaaagagt tttatggtcg aaagatttat gctggtattt taggagagat tgtgaatata
      121 cctgataatt ggggaaaatt tattaagcct aaagctggtt caaaagtctt tactggaaga
      181 gttgttaatg gaacccatga tttaataggt attggtctac ctttcgacta tcctatatgg
      241 attagtgagg ttgtagaatt catagctgaa tggcgttgtt ttgtgttaga tggtcgcgta
      301 ttagatgttc gaccctatac aggtgattat catgcacaat ttgatgcaag tgtaattgat
      361 gaagcgatat catgttggaa agatgcgcca atagcttatg gactagatat cggtgttact
      421 cgcgatggca gaacacttgt tgttgaagta aatgatggtt atgcattggg aaattatggc
      481 ctatctcctt taaaatcaat caattttcat agagctaggt ggaaagaaat ggtaaaatcc
      541 tattttgaaa aaaaatgaaa tttttaaaat ccaacaggat gttattttct aaaataaata
      601 tggtagataa ccaatataaa aagcatcaaa atgaacaagg aggaatctga tgcttggagc
      661 aattattgga gatattgtcg gttctgttta cgaatggaac aatatcaaaa caaaggactt
      721 tcctttattt cggaaggact gcttttttac agatgacacg gttatgacct gtgctgttgc
      781 agaagcaatt atgaatggtg gacaaaaaga cgatttcatt gacgcgatga agaaatatgg
      841 cagaatgtat ccgaatgctg attacggtgc tcggtttaat gcatggctaa acagcgataa
      901 ccgtgagcct tataatagct ttgggaatgg atcagctatg tgtatttctc catgtgcttg
      961 gatcatggac tgtggttttt atgcgaaaac tggtatgtgg ccatcatcta gaggacttac
     1021 gagtctttct gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg ctatggctac
     1081 agctgatgct atctttctat gtcgttttta ctttggtggt tattgtagag agtacgagca
     1141 atcaattaac gacaatccta cagagtgtaa aagacgaatt aaggattata tagaaaaaga
     1201 atacgattac aatctatctc aaactttaga tgaaatccgt cctaactatc gttttaacga
     1261 aacatgtcag gaaactgtcc ctcaagccat tatcgccttt cttgaaagta gagacttcga
     1321 agatgcgata agaaatgcca tctcacttgg tggcgacagt gatactctcg cagcaatcac
     1381 ttgtaacata gcagaggcag cttacggtat tcctgattgg atcaaggata aggcctattc
     1441 ttacttggat gaacccttaa aggatgtagt taggcgatgg gaaaatagaa taaaagcgta
     1501 ttaatctcca gtacgtcaga tttctctttt ctgtattttc attgtataat aggattacat
     1561 ttacttgcat acgacgaaat gtaaacattt ctgtttaaaa ttaaggataa aatcaatgaa
     1621 aaaagcaatg gtaattatca accctacttc tggtggcgag aaggctttgg attacaaaga
     1681 aaagctggag aataaagcaa aagaatactt tgaatatgtt gaaaccaaaa ttaccgaaaa
     1741 agcgctggat gcaacacatt ttgctgaaga agcttctcgt gagcagtatg atgcagtggt
     1801 tgtgtttggt ggagatggaa ctgtcaatga agtcatttca ggtattgatg agagagacta
     1861 cattcctaag ttagggatta tcccaggcgg tacgggtaac ctcattacaa aacttttgga
     1921 aatcaatcaa gacatcgatg gcgcaattga cgaactggat tttgatttaa ccaataagat
     1981 tgatatcggt aaagcaaatg acaactattt tggttatatc tttagtatcg gttctctgcc
     2041 tgaggcgatt cacaatgttg aaatagagga caaaacaaaa ttcggtattt taacctatgc
     2101 tgtaaatacc atgaagtctg tcatgacaga tcaggtcttt aacattaagg ttgagacaga
     2161 aaatggaaat tatgttggtg aagctagcca tgttttggtc cttttgacaa attacttcgc
     2221 tgataagaaa atctttgaag aaaacaagga cggctatgcc aacattttga ttctgaaaga
     2281 tgcctctata ttctccaaat tatccgtcat tcctgattta ttaaaagggg atgttatcgc
     2341 aaatgataat atcgagtata tcaaagcgcg taatattaaa atctcttcag atagtgaatt
     2401 ggagtcagat gttgacggag ataaatcaga taacctacct gtagaaatca aagtcctagc
     2461 tcagcgagta gaagtatttt caaaaccgaa agaggattag tatatagaga aagccttttt
     2521 taaggctttt tgtatacttt aaaagatagt tcctttaaca acggacattc cttgcaaata
     2581 gtttacaaaa atagtatact ggattcattg agtttgaaaa cgtttgcgta aaatttgaat
     2641 gaatacttta ggagacaaat tgatggaatt gagtgctatt taccataggc ctgagtcgga
     2701 gtatgcctat ctttataagg ataagaaact ccatattcga attcgaacta agaaagggga
     2761 cattgaaagc atcaacttgc actatgggga cccttttatc tttatggagg agttttatca
     2821 ggatacaaaa gaaatggtca agataacttc tggtacctta tttgaccatt ggcaggttga
     2881 agtgtcagtt gactttgcac gtatccagta tctctttgag ctcagagata cagaaggtca
     2941 aaatattttg tatggcgata aagggtgtgt ggaaaattct ctagaaaatc ttcatgcaat
     3001 tgggaatgga tttaagttgc cttatcttca tgagattgat gcctgcaagg ttcctgactg
     3061 ggtttcaaat acggtatggt atcagatatt tcctgaaaga tttgccaatg gcaatgctct
     3121 attaaaccca gaagggactt tagactggga ttcatctgtc acacctaaga gcgatgattt
     3181 ctttggtggt gatttacagg ggattattga tcatatggat tacttgcaag acttgggtat
     3241 tactggacta tatctttgtc ccatctttga atctacaagc aatcacaagt acaatacgac
     3301 agattacttt gaaattgacc gtcattttgg agacaaggag acctttcggg aactggtgga
     3361 tcaagcgcat catcgtggca tgaaagtcat gctggatgcg gtatttaatc atattggttc
     3421 gcaatctctt caatggaaaa atgtcgtcaa aaatggtgaa cagtctgctt ataaggattg
     3481 gttccatatt caacaattcc cagtgacaac tgaaaagcta gttaataaga gagacttacc
     3541 ctatcatgtt tttggtttcg aggactatat gcctaagcta aatacagcca atccagaggt
     3601 caagaattat cttttaaagg ttgcgactta ttggattgaa gagtttaata tcgatgcttg
     3661 gcgtttggat gtggctagtg agattgacca tcagttctgg aaggattttc gtaaggcagt
     3721 tttagctaaa aatcctgatc tttatatcct aggagaagtc tggcatacat ctcagccttg
     3781 gctaaatgga gatgagttcc atgccgtcat gaattatcct ttatctgata gtatcaagga
     3841 ctatttctta cgaggaatta agaagacaga ccagttcatc gatgaaatca attgagagtc
     3901 tatgtattac aagcagcaga tttcagaggt catgtttaat ctcttggatt cacatgatac
     3961 agagcgaatc ctgtggacgg ccaatgaaga tgttcaactg gttaaatcag ccttagcctt
     4021 tctcttttaa caaaaaggaa caccgtgcat ttattacgga accgagctag ccttgactgg
     4081 aggaccagat ccagattgtc gtcgttgtat gccttgggaa cgtgtatcaa gtgacaatga
     4141 tatgctgaac tttatgaaga ggctgattaa gattcggaaa tacgcgtcag taatcatttc
     4201 gcatggcaag tatagccttc aagaaatcaa atctgatcta gtagctctgg aatggaaata
     4261 cgaaggatgg atcctcaaag caatattcaa ccaatcaaca gaagattatc ttttagagaa
     4321 agaagcagta gcactagcaa gcaattgcca agaattggag aatcagcttg tcatctctcc
     4381 agatggattt gtgattttct aaaaactagt tgatgaagat tatggtacat ttcatatctt
     4441 atatagtata ataaggctag ttactaaact tgtaaaggag aacttaaatg aattgtagag
     4501 gacatgaaac aagacaaaga attgttagag attttgaagt ttagcctaaa gcacatatta
     4561 agctgttagc aaatcaacaa aaacatagtg atgcaggagc aactattgaa gatgaatatt
     4621 atgtatttat cgctgagagt aaaattgatg gcaagaagga agttattcag tgttgcatgg
     4681 gtgcggcaag ggatttttta gaactaatta atcacaaagg gctacctctt tttaatccgc
     4741 ttgtaggtga ttctcatgta aataatagac aagaatatga caatacaggg agtggaaatt
     4801 tacaacctga aaagtggaat gaaactgcaa agcagcttta taatgttata atgtggttga
     4861 ttattttatg gaatgctaag ccggatacac ctttatttaa tttt
//
LOCUS       DAVHIU010000116         4835 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVHIU010000116 DAVHIU010000000
VERSION     DAVHIU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.118"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            85..1260
                     /gene="mutY"
                     /locus_tag="VVN06_001771"
     CDS             85..1260
                     /gene="mutY"
                     /locus_tag="VVN06_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU5563264.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1315..2019
                     /locus_tag="VVN06_001772"
     CDS             1315..2019
                     /locus_tag="VVN06_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563265.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2012..3361
                     /gene="vicK"
                     /locus_tag="VVN06_001773"
     CDS             2012..3361
                     /gene="vicK"
                     /locus_tag="VVN06_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU5563266.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3363..4172
                     /locus_tag="VVN06_001774"
     CDS             3363..4172
                     /locus_tag="VVN06_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5563267.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4263..4505
                     /locus_tag="VVN06_001775"
     CDS             4263..4505
                     /locus_tag="VVN06_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU5563268.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4507..4761
                     /locus_tag="VVN06_001776"
     CDS             4507..4761
                     /locus_tag="VVN06_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU5563269.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
BASE COUNT         1495 a          831 c         1108 g         1401 t
ORIGIN      
        1 gatagaaaag ttatatacta tctattgaaa atattataaa tcagatacaa agacaggtga
       61 aaccttctgt gttatactag agatatgtta gatttgaaag aatacggtat cgtcatgtgg
      121 ccggaggaga aagtcatttc tttccgtgag aaacttctcg cttggtatga tgaaaacaaa
      181 agagatttgc cttggaggag aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg
      241 cttcagcaga ccagggtgga tacagttatc ccttactacg aaagattttt ggactggttt
      301 ccaactgtcg aaagtctggc aactgcgcct gaggagagtt tactgaaagc ttgggagggc
      361 ttgggttatt attctcgagt tcgcaatatg caggctgcag cccagcaaat tatgactgac
      421 tttggtggcc aatttccaaa tacctatgaa ggaatttcca gcttgaaagg gattggacct
      481 tacacagcag gagccatttc cagtattgct tttaacttgc ctgagccagc tgtagatggt
      541 aatgtcatgc gggtcttggc gcgtctgttt gaagtcaacc acgatattgg gattccaagt
      601 aatcgaaaaa tttttcaggc aatgatggag atcttgatta atccagatcg tccgggtgat
      661 tttaatcaag ccttgatgga cttagggtct gatattgagt ctcctgtaaa tcccagacct
      721 gaagaaagcc cagtcaagga ctttagtgcg gcatatcaga atggaaccat ggaccgttat
      781 ccaattaaat ctcctaagaa aaagccggtt cctatttatc ttaaagcctt agttgtgaaa
      841 aattctcaag gacaattttt acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt
      901 tggcatttcc cctttataga agttgataac ttttcgcaag aagagcagtt tgacctcttt
      961 catcaggttg cggaagaaag tgtgaactct ggccccagtc cagaagagag tttccagcag
     1021 gactatgacc tagatgttga ttggcttgat gtttgttttg atacagtcca gcatgttttt
     1081 agtcatcgaa aatggcatgt acagattgta gcaggtcagg tgagtgactt ccatgatttt
     1141 tcagataggg aagttcgttg gctttcacca gaagaattca agaattaccc acttgccaaa
     1201 ccccaacaaa aaatctggca ggcttatgca caagccaact tagacagtag ccaagactag
     1261 ctattgtgtc ttctgactat tttttggtat aatagtagag aaaaaggtga acatatgaaa
     1321 aaaatactaa ttgtagatga tgagaaacca atctcggata ttatcaagtt taatatgacc
     1381 aaggaaggtt acgaagttgt aactgctttt aatggtcgtg aagcgctaga gcaatttgaa
     1441 gcagagcaac cagatattat tattctggat ttgatgcttc cagaaattga tggtttagaa
     1501 gttgctaaga ccattcgtaa gacaagcagt gtgcccattc ttatgctttc agccaaagat
     1561 agtgaatttg ataaggttat cggtttggaa cttggggcag atgactatgt aacgaaaccc
     1621 ttctccaatc gtgagttgca ggcgcgtgtt aaagctcttc tgcgtcgttc tcaacctatg
     1681 ccagtagatg gtcaggaagc agatagtaaa cctcaaccta tccaaattgg ggatttagaa
     1741 attgttccag acgcctacgt ggctaaaaaa tatggcgaag aactagactt aacccatcgt
     1801 gaatttgagc ttttgtatca tttagcatcg catacaggtc aagtcatcac gcgcgaacac
     1861 ttgcttgaga ctgtctgggg ttatgactat tttggtgatg tccgtacagt tgatgtgact
     1921 gtacgacgtc tgcgtgagaa gattgaagat acgcccagcc gaccagagta tatcttgacg
     1981 cgccgtggtg tagggtatta catgagaaat aatgcttgat ttactgaaac aaaccatttt
     2041 taccagagat tttatcttta tcctgatttt gttaggtttc atccttgttg tgaccctctt
     2101 attactggaa aatagacgtg ataatattca gttgaagcaa atcaatcaaa aggttaaaga
     2161 tttgattgca ggagattatt ccaaggttct tgatatgcaa ggtgggtctg aaatcaccaa
     2221 tattaccaat aatttgaatg acttgtcgga ggttattcgt ctcactcagg aaaatctaga
     2281 acaagagagt aagaggctaa atagtattct gttttatatg acagatgggg ttcttgcgac
     2341 caaccgtcgg ggtcagatta tcatgattaa cgatacagcc aagaagcaac tggggttggt
     2401 taaggaagat gttctgaata gaagcatttt ggaattgctc aagatagaag aaaactatga
     2461 attgcgtgat ttgattaccc aaagtccaga attgttgcta gattcccaag atatcaatgg
     2521 cgaatatttg aaccttcgag ttcgctttgc cttgatacgt cgagagtctg gctttatttc
     2581 aggtttggtg gctgttttgc atgatacgac ggagcaggag aaggaagaac gcgaacgaag
     2641 actctttgtt tccaatgtta gccatgagtt acggactcct ctgactagcg taaaatccta
     2701 tcttgaagcc ttggatgagg gggctttgtg tgaaactgta gcaccagact ttatcaaggt
     2761 ttctcttgat gagaccaacc gtatgatgcg catggtgacg gatctcctcc atctttcacg
     2821 tattgataat gctaccagtc acctagatgt ggaactgatt aacttcactg cttttattac
     2881 ctttatcctc aatcgttttg acaagatgaa aggacaggaa aaggagaaaa aatatgagtt
     2941 ggtgagagat tatcccatca attctatctg gatggaaatt gatacagata agatgacgca
     3001 ggttgtcgac aatattttaa ataatgctat taagtattcg ccagatgggg gtaaaatcac
     3061 tgtcagaatg aagacaactg aagaccagat gattttatcc atttctgacc acggtttggg
     3121 gattcctaag caggatttac cacgtatctt tgaccgtttc tatcgtgtgg atcgtgctag
     3181 aagtcgtgca caaggtggta caggtctagg actgtctatc gctaaagaaa ttatcaaaca
     3241 acataagggc tttatttggg ccaagagtga atacggcaag ggttcaacct ttaccattgt
     3301 gctcccttat gataaggatg cagtgaaaga agaagtatgg gaggatgaag tagaagacta
     3361 gaatgagtga aataggcttt aaatacagta ttttagcgtc gggttccagt ggaaattctt
     3421 tttatctgga aacctcaaaa aagaagcttt tagtagatgc aggcttgtct ggcaagaaaa
     3481 ttaccagtct gctagctgaa attaaccgta agccagaaga cctggatgcc atcttgatta
     3541 cccatgagca ttcagatcat atccatggag taggcgtttt ggctcgcaag tatggtatgg
     3601 atctttatgc caatgaaaag acctggcaag ctatggaaaa tagtaaatat cttggcaagg
     3661 tggattcttc gcaaaagcat atttttgaaa tgggtaaaac caaaaccttt ggagatatcg
     3721 acatcgagag ttttggtgta agccatgatg cagtcgcacc gcagttctat cgctttatga
     3781 aggatgataa gagttttgtc cttttgacag atacaggtta tgtcagtgac cgtatggcgg
     3841 gcattgtcga aaatgcggat ggctatctta tcgaggccaa ccatgatgta gagattttgc
     3901 gatcaggttc ttacgcttgg cgactcaaac aacgaatcct atctgacctt ggtcaccttt
     3961 ctaacgagga cggtgctgaa gctatgattc ggacgctagg aaatcgtact aagaagattt
     4021 accttgggca tttatctaag gaaaacaata tcaaggaact ggctcatatg accatggtca
     4081 atcagctggc tcaagctgat ctgggagtcg gagtagactt taaggtttat gatacctcac
     4141 cagataccgc aacaccattg acagagatat agaaagaacg ctgagaaggt gttcttttta
     4201 tattgactga acacctaaaa agtaatacaa tggtgttacc attaaaaaag ggagcacaaa
     4261 agatgactac tattacatta aaagtttctg aagctgataa aacatttatg aaagcaatgg
     4321 ctaagtttga aggagtttcc ctttcggaac ttattcgcac caaaactctt gaagccctag
     4381 aagatgaata cgatgctcgt gtggcagatt tagcctatca agagtattta gaagacttgg
     4441 aaaaaggagt tgaacccatt acttgggaag aaatgatgca tgatttaggc ttgaaggatg
     4501 aataatttgt ataaattagt tccaacaaga cgtttcatca agcaattgaa aaaattggac
     4561 cgttatacgc agaagctaat tacaaactat ttacaaatca atgttttgga agacccaaga
     4621 cgacacggaa aggctttggt tggtaatcgc gttggtcaat ggcgctatag aattggtaat
     4681 tatcgagtta tcgtacaaat tgtagatgat gaattagttg ttgctactct agaagttggt
     4741 catcggagag atatttattg aattaaagct gagtaataac aatatagaaa gtaaggagag
     4801 taaaaatttg aagaaaaaat ggttttttgc agatt
//
LOCUS       DAVHIU010000117         4819 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVHIU010000117 DAVHIU010000000
VERSION     DAVHIU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.119"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..823
                     /locus_tag="VVN06_001777"
     CDS             <1..823
                     /locus_tag="VVN06_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5563270.1"
                     /translation="IFKYIPMYGVLIAFKDYNPLKGILGSDWIGFSEFTKFISSPNFG
                     ILLANTLKLSIYGLLLGFLPPIILAIMLNQLLSEKVKKRIQLILYAPNFISVVVIVGM
                     IFLFFSVGGPINNFLSMFGMKADFLTNPDFFRPLYIFSGIWQGMGWASTLYTATLVNV
                     DPALVEAARLDGANIFQRIWHIDIPALKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLN
                     LPTSEIISTYVYKVGLVSGDYSYSTAVGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            834..1724
                     /locus_tag="VVN06_001778"
     CDS             834..1724
                     /locus_tag="VVN06_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5563271.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            1753..3369
                     /locus_tag="VVN06_001779"
     CDS             1753..3369
                     /locus_tag="VVN06_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5563272.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFTFISGNGNEDFKFLFAAFDIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            3379..4698
                     /locus_tag="VVN06_001780"
     CDS             3379..4698
                     /locus_tag="VVN06_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU5563273.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAVKHMDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         1538 a          830 c          975 g         1476 t
ORIGIN      
        1 tatctttaaa tacatcccta tgtatggggt tttaatcgca tttaaagatt acaatccttt
       61 aaaaggaatt ttagggagtg attggattgg tttttctgag tttacaaaat tcatatcctc
      121 tcccaacttt ggtatcttgt tagccaacac attaaaatta agtatctatg gtttattgct
      181 tggcttttta ccaccaatca ttcttgcgat tatgctcaat caactcttga gtgaaaaagt
      241 caaaaaacga attcagctca ttttatacgc accaaacttt atctcagtcg ttgttattgt
      301 cggtatgatt ttcctcttct tttcagtggg aggaccaatc aacaattttc tttctatgtt
      361 tggaatgaag gctgacttct tgacaaatcc agacttcttt agacctttat acatctttag
      421 tggtatctgg caaggaatgg gctgggcttc aacgctctac acggcaacat tggtaaatgt
      481 agatccagcc ttagtagaag cagctcgact ggatggagcc aatatcttcc aacgaatctg
      541 gcacattgat attccagctc ttaagcctat tatggttatc caatttgttt tagctgcagg
      601 tggaattatg aatgtcggat atgaaaaagc attcttgatg cagacatcgt taaatttgcc
      661 aacttctgaa attatctcga catatgtcta taaagttggt cttgtatcag gagactattc
      721 ttactcaaca gcggttggtt tgtttaatgc agtgattaac gtagtattgc ttgttgcagt
      781 taaccaaatc gttaaacgca tgaataatgg tgaaggaatt taaggaggaa agtatgaaaa
      841 attcgattat ggatacaaaa tttgatagac gtatcttact cttaaataaa atcattattg
      901 tctttatcgt tttgatgact ttgcttcctt tactttatat cgtcgtagca tcctttatgg
      961 atcctaaggt tctggttagt agagggatta gctttaatcc agccgattgg actgtagaag
     1021 gttaccagcg tgtattcagt gaccaatcta ttctaagagg ttttatcaat tctctactat
     1081 actcttttgg atttgcagct ttaacagtat tgctatctgt gtttacagct tatcctcttt
     1141 ctaagaaaga cttggttgga cgtcgttgga ttaactactt cttgattgtg actatgttct
     1201 ttggtggtgg tttagtccca acttacttgc tcgtaaaaga attgggaatg ctcaatactc
     1261 catgggctat cattgttcca ggtgctgtca atgtttggaa tattattctt gctagggcct
     1321 atttccaagg attgcctgaa gaattagttg aagctgctgt cattgatggt gcaaatgatt
     1381 tacagatttt cttcaaaatc atgcttcctc ttgcaaaacc aattatgttt gttctcttcc
     1441 tctatgcttt tgtaggacag tggaactcat actttgatgc aatgatttat atcaaggatc
     1501 caaacttgga accattgcaa cttgtacttc gtaaaattct cattcagagc caaccaggtc
     1561 aagacatgat tggagcacaa gcggctatga atgaaatgaa acgtttagct gaattgatta
     1621 aatacgcaac tattgtcatt tccagcttgc cattgattgt tatgtatcca ttcttccaaa
     1681 aatactttga taaaggaatt atggctggtt cacttaaagg ataaaaaaag aaaaaataaa
     1741 aggagttttc tcatgaaatt caaaacattc tcaaaatcag cagttttgtt gacagctagt
     1801 ttagcagtac ttgcagcctg tggctcaaaa aatacagctt caagtccaga ttataagttg
     1861 gaaggtgtaa cattcccgct tcaagaaaag aaaacattga agtttatgac agccagttca
     1921 ccgttatctc ctaaagaccc aaatgaaaag ttaattttac aacgtttgga gaaggaaact
     1981 ggcgttcata ttgactggac caactaccaa tccgactttg cagaaaaacg taacttggat
     2041 atttctagtg gtgatttacc agatgctatc cacaacgacg gagcttcaga tgtggacttg
     2101 atgaactggg ctaaaaaagg tgttattatt ccagttgaag atttgattga taaatacatg
     2161 ccaaatctta agaaaatttt ggatgagaaa ccagagtaca aggccttgat gacagcacct
     2221 gatgggcaca tttactcatt cccatggatt gaagagcttg gagatggtaa agagtctatt
     2281 cacagcgtca acgatatggc ttggattaac aaagattggc ttaagaaact tggtcttgaa
     2341 atgccaaaaa ctactgatga tttgattaaa gtcctagaag ctttcaaaaa cggggatcca
     2401 aatggaaatg gagaggctga tgaaattcca tttacattta ttagtggtaa cggaaacgaa
     2461 gattttaaat tcctatttgc tgcatttgat ataggggata acgatgatca tttagtagta
     2521 ggaaatgatg gcaaagttga cttcacagca gataacgata actataaaga aggtgtcaaa
     2581 tttatccgtc aattgcaaga aaaaggcttg attgataaag aagctttcga acatgattgg
     2641 aatagttaca ttgctaaagg tcatgatcag aaatttggtg tttactttac atgggataag
     2701 aataatgtta ctggaagtaa cgaaagttat gatgttttac cagtacttgc tggaccaagt
     2761 ggtcaaaaac acgtagctcg tacaaacggt atgggatttg cacgtgacaa gatggttatt
     2821 accagtgtaa acaaaaacct agaattgaca gctaaatgga ttgatgcaca atacgctcca
     2881 ctccaatctg tgcaaaataa ctggggaact tatggagatg acaaacaaca aaacatcttt
     2941 gaattggatc aagcgtcaaa tagtctaaaa cacttaccac taaacggaac tgcaccagca
     3001 gaacttcgtc aaaagactga agtaggagga ccactagcta ttctagattc atactatggt
     3061 aaagtgacaa ccatgccaga tgatgccaaa tggcgtttgg atcttatcaa agaatattat
     3121 gttccttaca tgagcaatga caataactat ccaagagtct ttatgacaca ggaagatttg
     3181 gacaagattg cccatatcga ggcagatatg aatgactata tctaccgtaa acgtgctgaa
     3241 tggattgtaa atggcaatat tgatactgag tgggatgatt acaagaaaga acttgaaaaa
     3301 tacggacttt ctgattacct cgctattaaa caaaaatact acgaccaata ccaagcaaac
     3361 aaaaactaga ggttgattat gggagataag aaatacacag tagaaaaagc caatcgtttt
     3421 atagcagaaa ataaacatct cgttaatact caatataagc ctgaagaaca tttttcagct
     3481 gagattggtt ggatcaatga tccaaatgga tttgtctatt ttcgtggaga ataccatctc
     3541 ttttatcaat tctatccata tgatagtgtt tggggaccta tgcactgggg acatgctaaa
     3601 agtaaggact tggtgacttg ggagcacttg ccagtggcac ttgctcctga ccaagattat
     3661 gaccgaaatg gttgtttctc aggctctgcc attgtcaagg atgatcgcct ctggctcatg
     3721 tacactgggc atatcgaaga aaaaaccggt gtccgccaag tgcaaaatat ggcattttca
     3781 gatgacggga ttcactttga aaagatttcc caaaatccag ttgtaactgg aacagacttg
     3841 ccagatgagt tgattgctgc tgatttccgt gatccaaaac tctttgaaaa agatggacgc
     3901 tattactccg tagtagctgt caaacacatg gataatgtgg gctgtatcgt tctactaggg
     3961 tccgataacc tagtagaatg gcagttcgaa tccatctttt taaaaggggg agaacaccaa
     4021 ggctttatgt gggaatgccc agattacttc gagttagatg ggaaagattg ccttattatg
     4081 tcacccatgc gttatcagcg tgagggagac tcatatcata acatcaactc atcgcttttg
     4141 ttcacgggta aggtagattg gagagaaaaa cgttttatcc cagaatcagt tcaagaaatt
     4201 gatcatggcc aagacttcta tgcgcctcaa acattgttgg acgatcaaaa tcgtcgtatc
     4261 ctgattgctt ggatgcagac atgggggcgt acccttccaa cccatgacca agaacacaaa
     4321 tgggcatgtg ccatgacttt acctagaatt ctaagattgg aagatggcaa actaagacaa
     4381 ttccctgtta aaaaaggcca atatcaaatc caaatagata aagattgtca ttaccactta
     4441 ggaaatgata tagattatct tgaatttggt tatgacagta atgcgcagca agtttacatt
     4501 gatcgtagcc atcttattca aaaaattcta ggtgaagaag aacaggacac tagtcgacgg
     4561 tatgtagata ttgaagctaa agaattggaa gttgttctag ataaaaattc catcgagatt
     4621 tttgtcaatc aaggtgaagc aagcttgact gcaacttatt acttaacggt gccagctgag
     4681 ctatcacgaa ttgattaaaa attaagttat ttctcctaaa gaaaaagttc tctttctaaa
     4741 atagtggaaa gaggactttt tgtgttttgg gtatataagc ttagtttatg gtatttgtaa
     4801 aattggtgtt ggattatga
//
LOCUS       DAVHIU010000118         4727 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVHIU010000118 DAVHIU010000000
VERSION     DAVHIU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.120"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(67..1890)
                     /gene="dnaK"
                     /locus_tag="VVN06_001781"
     CDS             complement(67..1890)
                     /gene="dnaK"
                     /locus_tag="VVN06_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5563274.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2370..2894)
                     /gene="grpE"
                     /locus_tag="VVN06_001782"
     CDS             complement(2370..2894)
                     /gene="grpE"
                     /locus_tag="VVN06_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5563275.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(2921..3955)
                     /gene="hrcA"
                     /locus_tag="VVN06_001783"
     CDS             complement(2921..3955)
                     /gene="hrcA"
                     /locus_tag="VVN06_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5563276.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4119..4628)
                     /locus_tag="VVN06_001784"
     CDS             complement(4119..4628)
                     /locus_tag="VVN06_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU5563277.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         1255 a         1060 c          904 g         1508 t
ORIGIN      
        1 tatcttttca tgataatctg caatgtcaga aagattattt ttattaacac tcataacact
       61 agccccttat ttttcagtaa actctccgtc tacgacgtca tcgcctgcgt ttcctgttgc
      121 ttgtgcgcct tctgctcctt cttgagcttg ttgcgctgct gcggcttgtt cgtagagttt
      181 cacagcaagg ccttgagctt tttcgttcaa cgcttcaagt ttcgctttca tgtcgtccaa
      241 gttgttgtct tcttgagctt tcttaaggtc atcaagggca gcttgggcag cgtcacgttc
      301 tgcgtcgaag cctttacctt cagtttcctt gattgtcttt tcagtcgcaa agattgcttg
      361 gtctacttca ttacgaaggt ctacttcttc tttacgtttc ttatcagctt cagcgtttgc
      421 ttcggcatct ttcatcatgc ggtcgatttc ttcgtcagtc aaacctgagt tcgattggat
      481 gacaatagtt tgttcttttt gagttccaag gtctttggcc ttaacagaca cgataccgtt
      541 cttgtcgatg tcaaatgtta cttcgatttg aggaattcca cgaggtgcag ctgggatatc
      601 agtcaattgg aagcgtccaa gagtcttgtt atctgctgcc attgggcgtt caccttgaag
      661 aacgtggata tcaacggctg gttggttgtc tgctgctgtt gagaagactt gtgatttaga
      721 tgttgggatt gtagtgttgc gatcgataag ttttgtaaat actccaccca ttgtttcgat
      781 accaagtgac aatggcgtta catcaagaag gacaacgtcc ttgacatcac cagtaatcac
      841 accaccttgg atagccgcac ccatagcaac tacttcatca gggtttactg atttgtttgg
      901 ttctttacca gtttcagctt taacagcttc aacaacggca gggatacgag ttgaaccacc
      961 aacaaggata acttcgtcga tttctgacaa gctcaaacct gcatctgaaa gggcttgacg
     1021 aactggaact tttgtacgtt caacaaggtc acgagtcaaa tcgtcaaatt tcgcacgagt
     1081 caaagtcatt tccaagtgaa gaggtccagc ctcacctgca gtgataaatg gcaagctgat
     1141 ttgtgttgaa gttacaccag aaaggtcttt cttcgctttt tcagccgcat ctttcaaacg
     1201 ttgcattgcc atcttgtcag tagacaagtc gataccgttt tctttcttga attctgctac
     1261 caagtggtca atgatttttt ggtcaaagtc gtcaccacca agtttgttgt cccctgcagt
     1321 tgacaatacg tcgaagacac cgtcacccaa ttcaaggata gagacgtcga atgtaccacc
     1381 accaaggtca aataccaaga ttttttcttc tttgtcagtc ttgtccaaac cataagcaag
     1441 agctgctgca gttggttcgt taacaatacg ttctacttca agaccagcaa ttttaccagc
     1501 gtcttttgtt gcttgacgtt gagcgtcgtt gaagtaagcc ggaactgtga taacagcttt
     1561 ggttactttc tcaccaaggt agtcttcagc gtagcctttc aagtattgaa ggatcatagc
     1621 tgagatttct tgtggagtgt attcttttcc atttgcagaa actttttcag aagttcccat
     1681 cttagatttg atagagataa ctgtatctgg gtttgtgact gcttgacgtt ttgcagcatc
     1741 accaacgata atttctccgt ttttgaatga gactacagat ggagttgtgc ggtttccttc
     1801 tgggtttgcg atgattttgc tttcagttcc ttcaagaact gcaactgctg agtttgttgt
     1861 acctaagtca ataccgataa ttttagacat gtgtttttct ccttgttagt taattttcta
     1921 tttttctttt tgatactctt cttaagtggc gacgccgtca gagcttgact ttgtcaatat
     1981 ctttgactaa actttgagcc taaggtctca aaatttgcgc aaatagcgcc acagcgaaga
     2041 gtatcgtctt tggttcgctt cgctcactat tacaaagcca aacatatttt atctttcttc
     2101 atagtttatt gtcgtttcgg acaagttttc tattctgtaa attgcgacac gaggagtcga
     2161 aatcgatttt atttcgacga cgagttagta aggaagctag acaaacgcca tagcgattgc
     2221 cgttttctga cgagttgctt tagctaccgt cagaattgcc gaatcgaaca cgccctaagc
     2281 tctgagtaaa aaagatagat tttcttgtgt tcatcgaaca cttcgtcaat ctcctatttt
     2341 tactgcgagc tttttacggg ctttgtatct tagttataca ccactaccat tgctgggcgt
     2401 aggatgcggt catggagttt gtagcctttt tgaaagactt gggcgatggt atctactggg
     2461 tgttcatcgt ctgctgggag agtttggatg gccatatggt agttatggtc aaattcgcca
     2521 tctgctgcga tttcttcaat tccttcttct ttcaaagcgt gaatcaagct ttcttgcacc
     2581 atccccaagc ccttcttcac atcatctgtc aaaccttcaa ctgcaagtgc acgctcaagg
     2641 ttgtcaagag atggtaagat tgcttttgcc aagtcctggc tacgataacg ttgcaagttt
     2701 tgacgttctt cattggcacg gcgttggata ttttgcattt ctgcatgagc gcgaagatat
     2761 ttgttttcga actcatctgc acgttcattt gccaagtcca actcagactt ttcaggagtt
     2821 gtttcttcag ctgtttccac aacttcctct tcttgaactt cttctacttc ttcatttttt
     2881 atatcttggg ccatgttcgc ctcctttaat gatttcaatc ttaatgtact tcataatgat
     2941 tactgctgag gtagcggtaa aaatctgtca acttcatagt caaaacacgg ttgaccacat
     3001 tgacttgatt gattagctgt tggtaatcca gattaactgg accgataatg gctagaattc
     3061 caactccccg ataaggaatg aggaacttac tactaatcac cgctaggtca gctaaacagg
     3121 actcttgacc gtctgcaaca cgaacatttt gcatctgttc ctcacgcaac ccctcacgaa
     3181 tctccaaggc caccttttgc ggttggtcaa agaactgata ggctgctaga ttggcaaaat
     3241 tcaagagatg gaccttgccc gccatcacaa tgttttcgtt gaacatttcc ttaaagatgt
     3301 gttcaaagag atcgatgaca ttatccgttg ttgtaaagta acgctggata atctgcggaa
     3361 tctccgtccg aatcttgtag tgaatatcta aaacggtgtg accgaggaaa cgttcctgaa
     3421 tgatgctctt cagtttcagc aaatcctcct gcaagaagtt ccttggaatc agaaactgac
     3481 tagtaaccgt tcgcgactcg tctagggtaa ataccgccaa ggctgtatgt tgccccaaaa
     3541 caacgatatc aaaggctgtc aaacgttgcc tgctcggctc aacatccagt gccactaccg
     3601 tacagccact caggtctgtt agtaagttag cagcctcttg cagaatatcc tccaatttga
     3661 agaattcctg atcaaaggct ttgacaatct catatacctc attttcagcc agccggtcaa
     3721 aatccagtga gtgtttcaca tagtactgaa aaccagcaac acttggcatc cgaccgcttg
     3781 aagtatgagc cttctcaagc aacccttgtt tttctagttc cgccatgtca ttacgaatgg
     3841 ttgcactgct agagttaata gactcttgca aggcttttga tccgacaggt tcgtgcgttt
     3901 tggtaaagat gtcaataatc agatttaaaa tatcctgctg acgctctgta accatctcat
     3961 cactcctctc tgtctgatca attagcactc aatgcatcca agtgctaact tatgattcta
     4021 ttatacaccc ttagaagaga atgtcaagac aaaaactcaa aaaattagca ctcttttatc
     4081 aagagtgcta aaaatcaatc tgaagaccta gaaccttgct attcatctac tcggtatttc
     4141 ctttttagtc tgaaataacc atgaattaga taagaaatca gaaaagcata taaaacaaaa
     4201 ataagaaaga aagacataga aagatcctct ctaaacaaac tcgtacaaac aacaagacca
     4261 cctaagaaag tagctgtaca ggaacgaagt ctagatcgca taacttccca tttgagcccc
     4321 ttactcatat agacttgatc ctctggaagt aaatcagaag acgatttacg aagaatcgaa
     4381 caagaacctg ctcctatcaa ttcccaacct ctatctctaa aatcttgcag ttcatgctta
     4441 tattttttaa gaaatctaga atcatagata cggtagatga catcgtctgg ttgacattgg
     4501 tcaaaataga acaaaccaaa acgactcgtt ctatacctcc aacctttcaa atgcatctca
     4561 tgtaaatatt cttcttcctt gtccaaatca acaatggtga aaatccgaaa ttctactctg
     4621 ctattcattg tcttacccca aaattagaaa acatgcctgg cgttatttat tagataattc
     4681 tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcg
//
LOCUS       DAVHIU010000119         4692 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVHIU010000119 DAVHIU010000000
VERSION     DAVHIU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.121"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(121..192)
                     /locus_tag="VVN06_001785"
     tRNA            complement(121..192)
                     /locus_tag="VVN06_001785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(157..159),aa:Arg,seq:cct)
     gene            complement(234..581)
                     /gene="rplS"
                     /locus_tag="VVN06_001786"
     CDS             complement(234..581)
                     /gene="rplS"
                     /locus_tag="VVN06_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5563278.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(700..1029)
                     /gene="crcB"
                     /locus_tag="VVN06_001787"
     CDS             complement(700..1029)
                     /gene="crcB"
                     /locus_tag="VVN06_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5563279.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1023..1397)
                     /gene="crcB"
                     /locus_tag="VVN06_001788"
     CDS             complement(1023..1397)
                     /gene="crcB"
                     /locus_tag="VVN06_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5563280.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1394..1660)
                     /locus_tag="VVN06_001789"
     CDS             complement(1394..1660)
                     /locus_tag="VVN06_001789"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU5563281.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1776..2219)
                     /locus_tag="VVN06_001790"
     CDS             complement(1776..2219)
                     /locus_tag="VVN06_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU5563282.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2323..3258
                     /locus_tag="VVN06_001791"
     CDS             2323..3258
                     /locus_tag="VVN06_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU5563283.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3312..3596
                     /locus_tag="VVN06_001792"
     CDS             3312..3596
                     /locus_tag="VVN06_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU5563284.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3653..3871)
                     /locus_tag="VVN06_001793"
     CDS             complement(3653..3871)
                     /locus_tag="VVN06_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563285.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3868..4692)
                     /locus_tag="VVN06_001794"
                     /pseudo
     CDS             complement(3868..4692)
                     /locus_tag="VVN06_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1444 a         1039 c          851 g         1358 t
ORIGIN      
        1 gattgcccaa atttttcaat aataaaacgc ataatattat ggagacacac aaaatcacct
       61 atattatgcg ttcttttaat atcaaagact ttttgacaaa cttctttgat gtctaattac
      121 tgccccctgc aggaatcgaa cctgcaacta ctccttagga gggagttgtt atatccattg
      181 aactaaggga gctagataaa aactctgctg aaaagcagag ttttttagtc gaattaacga
      241 cgaatttctt tgatacgagc tgctttacct tgaagagcac gcaagtagta caatttcgca
      301 cgacgtactt taccgtaacg aacaacttcg attttttcaa cacgtggagt gtggattggg
      361 aagatacgct caacacctac accgttagag attttacgaa ctgtgtagtt ttctgagatt
      421 ccagcacctt tacgtgcgat aacaacacct tcaaaaatct ggatacgttc acggttacct
      481 tcgacaactt tcgcatgtac acgaacagtg tcaccaggac ggaatgatgg gatatctgta
      541 cgaagttgac cttcagtcaa gctttggatt aatggattca ttttattctc ctatctttgt
      601 caatcttgag gaatcttcct cagcggataa actgtatttt tgtgcgtcca ttacacacaa
      661 gatatagttt accaaatttc taaaaaaaag taaagaaatt tatagcaaaa ttcctaaaaa
      721 aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa gaataaaaga ccttcttatc
      781 acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa gttgttaaac cgccacaaaa
      841 tcctgttgct agaatagcat atacttcctt agactctaca tgattgtaga atactccaat
      901 taaaaaacaa cctaaaagat tagctatgag cgttccgagt ggcaatttag aagcttgatt
      961 atagcgagaa aagaaatacc gtacgagtgc tcccaagcca caggcgattg caagatagac
     1021 gattaccatt tcttcctccc caaatagtaa gctaaaagca ggcctccacc gatactcaaa
     1081 agcaaataca gtatcaaact aagataacgc cctgtatcaa gcagcttcac agtatcaagc
     1141 attaggctgg aaaaagttgt taaacctccg caaaaacccg tccccagtgc taaaatcaag
     1201 cccttactag tccccttata gaccagatag cccttgacaa gataaatcaa gcagaaaatt
     1261 cccagatagt tgacaaagag agtgccccaa ggaaagtctg gactggctgg taaccaggtg
     1321 gaaactagat atcggacaag tccgcccaac atagcagcta gaaaaattcc tagcggataa
     1381 aattgttctt ttttcatttg atattttgat cctgataatc acgcgaacgt ttgagtatgt
     1441 ccgaaaaagt cgctacaata gtctcctgat agcgcttgtc ctctacacga ctcctaacct
     1501 tttcaaaaat aacttcttct ctcttagtat ctaaaatcgg tttacctgaa gctttcttat
     1561 aagcaaccac cccctcaact aaatgcattc gttcttctaa cagtttgaca atttgatcgt
     1621 cgatttgatc gatttcttga cgaataatat ctaaatccat acagtctcct cctttatttg
     1681 aattattgta tcaaaaagcc atcaattagg ctagtaaaat cccaactctc gataagaaaa
     1741 atgcactgat tgattacatc agcgcacttt tatgtttatc ctactttagc agccaattct
     1801 tctgcgaatt gttccaagcg ttcaatatct tcttcctcgg cagaaagatc aactttaaca
     1861 cactctgaac ctttttctgc tcctgttgac acaaagacac ggtcaaagtc atcaacagcc
     1921 ttacagaatt catcgtagaa ggtatctcct gagccgacca ctccgtagat tttgccattc
     1981 aagttgagat ctgctaggtc ttcgtagaag tccatcatct catctggcaa ttcaccatca
     2041 ccataagtat aggtcgcaac gatagcgatg tctgcttcca agaagtctga agcgtcaaca
     2101 gttgtacatt catcaacatc aacatccaag cccaagtcac gtaatttgtc tgctacaata
     2161 tctgcaattt cttcggtatt accggtcata ctggcaaata caatttttgc taatgccata
     2221 tcgtcctcct caatttatct ttctccatta tatcacatct ttttcatttt aaaaataaaa
     2281 attcttgtgc tacaatgaaa tgagaaagaa ttgaggttat ttatggagat ttgccaacaa
     2341 attttagaaa aaattaaaga atacgacacc attatcattc atcgtcatat gaaaccagac
     2401 cctgatgcct tgggaagtca ggtgggattg aaagccttgc tggaacatca tttcccagaa
     2461 aaaaccatca aagccgtcgg ttttgatgaa ccaactctta cttggatggc tgagatggat
     2521 cttgttgaag atagagccta ccaaggcgca cttgtcatcg tctgtgatac agctaatact
     2581 gctcgtatcg atgataagcg ctatagtcaa ggtgattttc tcattaagat tgaccaccat
     2641 ccaaatgatg atgtatacgg tgacctgtct tgggtcgata ctagttcaag tagcgctagc
     2701 gagatgatta ccctatttgc ccaaacaacc caactagcct tggcagatcg cgatgctgag
     2761 ttgctctttg caggaattgt cggtgataca ggtcgcttcc tctacccttc taccactgca
     2821 cggactcttc gcctggctgc ttatttgaga gaacataact ttgactttgc ggctctcact
     2881 cgcaaaatgg acactatgag ctacaaaatt gctaaactgc aaggctacat ctacgaccat
     2941 ctggaagtgg atgaaaatgg tgctgctcgc gttatcctga gtcaagaagt cttgaaacaa
     3001 tacaatgtaa ccgatgctga aactgcagcc attgtcggtg cacctggacg cattgacaga
     3061 gtgagtctct ggggaatttt tgtcgaacag gctgatggcc actaccgagt tcgcttacgc
     3121 agtaaaatcc atcctatcaa tgaaatcgcc aaggagcatg atggtggagg ccaccctcta
     3181 gcaagtggtg ctaattccta tagcctagaa gaaaacgaaa tcatctacca aaagttaaaa
     3241 aacttgctta aaaactgata aaatacttgc caaacttttc agaatctgat agactagtac
     3301 ggtaacaatc tatggctcgc aaagagacca tggcagaaag gaaatattgc aaaatgaaaa
     3361 aagatatcca tccagaatat cgcccagttg tcttcatgga cacaactact ggttaccaat
     3421 tccttagcgg ttcaacaaaa cgctctaacg aaacagttga gttcgaaggc gaaacttacc
     3481 cattgatccg tgtggaaatt tcatcagact cacacccatt ctacactgga cgtcaaaagt
     3541 tcactcaagc agatggacgc gtggatcgtt tcaacaaaaa atacggtctc aaataatgat
     3601 aagagaacag tttcggctgt tcttttttgt ttcttgaaat caactgctgt tttcatgttc
     3661 cagactcatc tgtaggttcg gtttccatgc tactaggcag gaaggaaata gctgtttcaa
     3721 cacgtccata atgagctgta ctattgtcac gaaccacact ttcattgatg gtccaagtgg
     3781 aattcatttt cttaaaagct tctcggactt tttccaaatc tttggaggca atggcctgct
     3841 ctaaggtttc aaaacgagga cttatactca tctgctttca caaagcattc tagtccatct
     3901 ccgattaacg atggacttta tcacctcctt ctccagtcct tgtatgacat cttgaagttg
     3961 attcatgaca tcttccaaag tttgaaaggc tttattctta aatccacgtt tacgaatctc
     4021 tttccacact tgttcaatgg ggtttatctc tggtgtgtat ggaggaataa atgcaaagcc
     4081 aatattagtc ggaatcttta aggtacttga tttatgccat atagcattgt ccataacgag
     4141 taaaagataa tcatctggat aagcttgtga aagctcttct aaaaaggcgt tcatccactc
     4201 agtattacat ccaccagctc ttaagaaaaa tgattcgcct gtatgggcat caacagctcc
     4261 ataacaatag tgaaattctc gtatatagtg actatggaca tgtggaccta ctcctattgg
     4321 agcccaacaa gatcccagtt tactgattct accgaaacca gcctcatctt ggtacatcaa
     4381 gcgaacctta tgaaaaggtc tactggtttt aaaacgcttt cttttcttct tgaattgaga
     4441 ttttattttt agacgcgaca atggtttgag cgtctgcttt cctaggatgt tctggacgtg
     4501 gcataatatt tcgccaacca tggcgcttca acagttgata gaaggcatca cgtgtgtagg
     4561 aacgatataa gcctgaaata aggtattgta acaaattctc ctgcctctgt agccttcaaa
     4621 tggcgggcaa gaaagacttt ctcttcctca actgtcatat atgcatggtt acgaccacca
     4681 cgtgtttctt ga
//
LOCUS       DAVHIU010000120         4612 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVHIU010000120 DAVHIU010000000
VERSION     DAVHIU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.122"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            51..848
                     /locus_tag="VVN06_001795"
     CDS             51..848
                     /locus_tag="VVN06_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU5563286.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            996..1118
                     /locus_tag="VVN06_001796"
     CDS             996..1118
                     /locus_tag="VVN06_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU5563287.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1140..2180
                     /locus_tag="VVN06_001797"
     CDS             1140..2180
                     /locus_tag="VVN06_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU5563288.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2351..3625
                     /locus_tag="VVN06_001798"
     CDS             2351..3625
                     /locus_tag="VVN06_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU5563289.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3635..4513
                     /gene="truB"
                     /locus_tag="VVN06_001799"
     CDS             3635..4513
                     /gene="truB"
                     /locus_tag="VVN06_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5563290.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
BASE COUNT         1372 a          841 c         1057 g         1342 t
ORIGIN      
        1 acctaaagtt tgtgatataa ttatcaagaa aaaagattta ggagtttatt atggtttctt
       61 cggaatttat ctcaaagatt gaatttgctt gcaataagaa agaaagtctt tatagtcaaa
      121 gcaaatttaa gtatgcgatt cgttcgatgt tcgcaggtgc atttttaacc ttcagtactg
      181 ctgcaggtgc agttggggct gacttgatta ataaaattgc accaggtagt ggacgcttcc
      241 tctttccatt cgtttttgct tggggcttgg cctacattgt ttttttgaat gccgagttgg
      301 tcacttcaaa catgatgttc ttgactgctg gtagtttctt aaaaaaaatc tcttggagaa
      361 aaacagctga gattttacta tactgtacct tgttcaacct tatcggagcc ttgatagcag
      421 ggtggggctt tgctcattcg gcagcctatg cgaatctgac acacgatagt ttcatctcag
      481 gtgttgttga gatgaagtta ggccgctcaa atgaattggt cttgcttgag gcgattttgg
      541 caaatatttt tgtaaatatt gcgattctgt catttatttt ggtcaaagat ggtggtgcca
      601 aactttggct tgtgttgtca gctatttaca tgtttgtatt cttaacaaac gagcacattg
      661 cggcgaactt tgcttctttc gcgattgtga aattcagtgt tgctgcggat tcaattgcca
      721 acttcggtgt tggaaatatg cttcgccact ggggtgtgac tttcatcgga aactttatcg
      781 gaggaggcct cttgatgggt cttccatatg ccttcctcaa taaaaacgaa gatacttatg
      841 tagattaaga aaatgagcac gattgagtcg tgcttttttc attttcaaaa taaggtaata
      901 gctatttctt atatcaaaat atagaaaact gatatttgta aactataact caaggtgcta
      961 caatatcctt aataaaataa tatggaggtc accttatgac ttgtgatttt aaatttgaaa
     1021 ctctacaact acatgctggt caagttgtgg ctccagctac taagtctcgt gcagtgccga
     1081 tttatcaaac aacatttttt gtttttgatg acacgtagga aggtgccgat ctgtttgcct
     1141 tgaggaaacc agggaacatt tatactcgta tcaccaatcc tacaacagct gcccttgaag
     1201 gtggtgttga agcgctagca acagcatcag gtatgactgc agtgacttat actattttgg
     1261 cgattgccca tgctggtgac catgtagtgg ctgcttcgac tatttacggt ggaaccttca
     1321 atcttttgaa agaacccctt cctcgttatg gtatcacaac aacctttttc gatattgata
     1381 atttggagga agtagaagca gctatcaaag acaataccaa gcttgtcttg attgaaacct
     1441 tgggtaaccc cttgattaat attccagacc tggaaaaact ggcagagatt gctcataaac
     1501 atcaaatccc acttgtgtca gacaatactt ttgcaacacc ttatttgatt aacgtcttct
     1561 ctcatggcgt tgacattgcc attcactctg tgactaagtt tatcggtggg catggtacaa
     1621 ctattggagg aataattgtc gatagtggtc gttttgactg gacggcttca gggaaattcc
     1681 ctcaatttgt tgacgagggt ccaagctgcc acaatttgag ctatactcgt gatgtgggtg
     1741 cagcagcctt tattatagct gttcgagttc aattgcttcg tgatacaggt gcagccttgt
     1801 caccattcaa tgctttcctc ttgctacaaa gacttgaaac ctcttcactt cgtgtggaac
     1861 gccatgtaca aaatgctgag acaattgttg attttcttgt caaccatcct aaggtagaaa
     1921 aggtaaatta tccaaaactt gcagatagtc cttatcatgc cttggctgag aaatacttgc
     1981 caaaaggtgt cggttcaatc tttaccttcc acgtcaaagg tggcgaggaa gaagcacgca
     2041 aggtcattga taatttagaa atcttttctg accttgcaaa cgcggcagat gctaaatcgc
     2101 ttgttgtcca tccagcaaca accactcacg gtcaattgtc agaaaaagac ctagaagcag
     2161 caggtgtcac accaaactaa attcgtttgt caatcggtct tgaaaatgta gaagatttga
     2221 ttgaagactt gcgcttggcc ttggaaaaaa tttaaagtaa aagaagataa acagtgggct
     2281 tcgactcact gtttttgatt ttccctcagg catgatataa tggttaaaga agtctagaaa
     2341 gaggaacgat atgaacgaaa tcaaatgtcc caactgtggg gaagtcttta cagtaaatga
     2401 gagtcagtat gccgaactct tgtcccaagt gagaacggca gagtttgata aggaactaca
     2461 cgataggatg aagcaggaac tggccttggc tgagcaaaag gccatgaatg agcaacagac
     2521 taaactggct cagaaggatc aagaaattgc gcaattacag agtcagatcc aaaactttga
     2581 tacagaaaaa gaattggcca agaaagaggt tgaacagaca agccatgagg ctctcttggc
     2641 taaggacaag gaagtacagc tcttagaaaa tcagttggct accttgcgtt tggagcatga
     2701 aaatcaacta caaaagaccc tttctgacct agaaaaagaa cgggatcagg ttaaaaacca
     2761 actacttttg caggaaaagg aaaatgaatt atctttggct tctgttaagc aaaactacga
     2821 agcccagctc aaggcagcta gtgaacaagt cgagttttat aagaatttta aggctcaaca
     2881 atctacaaaa gcgattgggg aaagcctaga acagtatgca gagagtgagt ttaacaaggt
     2941 tcgtagtttc gcctttccaa atgcttactt tgagaaggat aacaaggtct cttcgcgtgg
     3001 gtctaaaggg gactttatct tccgtgagtg tgatgaaaat ggagttgaaa tcatttctat
     3061 catgtttgag atgaaaaacg aagcggacgg aacagagaag aagcacaaga atgcagattt
     3121 ttacaaggaa ttggacaagg accgtcggga gaagaactgt gagtatgccg ttttggtgac
     3181 catgcttgag gctgataatg actactttaa cacagggatt gttgacgtca gtcacgagta
     3241 tgaaaaaatg tatgttgttc gtcctcaatt ctttatccaa ttgattggtc tcttacgtaa
     3301 tgcggcgcta aattccctaa aatacaagca ggagttggcc ttggttcgcg agcaaaatat
     3361 tgacattacg cattttgagg aagatttgga tgcctttaag ctagcttttg ctaagaacta
     3421 taattcagct tcgactaact ttggaaaagc tattgatgaa atcgacaagg ccatcaaacg
     3481 catggaagag gttaagaaat tcctgaccac atctgaaaac caactccgtt tagctaacaa
     3541 caaattggaa gatgtctctg ttaaaaaatt gacccggaaa aatccaacaa tgaaagcgaa
     3601 gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac ggtattatta acttaaaaaa
     3661 ggaagcagga atgacctcgc atgatgcggt ttttaaactg cgtaagattt tgggaaccaa
     3721 gaaaattggt catggtggaa ccttggatcc ggatgtggtg ggtgttttgc cgattgcggt
     3781 tggcaaggcg acacgcatgg tcgagtttat gcaggacgag ggtaagatct atgaggggga
     3841 aatcactctg ggctattcca cgaagactga ggatgctagt ggggaagtgg tcgcagaaac
     3901 ccctgttttg tctctcttgg atgaaaagct tgttgatgaa gcgattgcta gcttgactgg
     3961 gcctattact cagattcccc ctatgtattc ggcagttaag gttaatggtc gcaagctcta
     4021 tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa cgtcaggtga ccatttatca
     4081 atttgagcga acaagtccga tttcttatga tggccaactt gcccgattca cttttcgtgt
     4141 aaaatgcagt aaagggacgt acatccgtac tttgtcagtt gatttgggtg aaaagcttgg
     4201 ttatgcggct catatgtccc atttgactcg tactagtgct gctggcttac aattagaaga
     4261 cgctcttgcc ttggaggaaa ttgctgaaaa agtagaggct gggcaattag attttctcca
     4321 tcctttagag attgggacag gtgaccttgt caaagttttc ctaactccag aagaggctac
     4381 agaagttcgc tttggtcgtt ttattgagct agaccaaacg gacaaagaac tggctgcctt
     4441 tgaagatgat aaattgttag ccattctaga aaaacggggc aatctctata agccaaggaa
     4501 ggtttttagc tagatcgttt aggaataaaa atcgggtgat agataacaat tgcttgataa
     4561 aaccccatac taatagtaga atggttttgg gaattataat attccaattg tt
//
LOCUS       DAVHIU010000121         4591 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVHIU010000121 DAVHIU010000000
VERSION     DAVHIU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.123"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            37..471
                     /locus_tag="VVN06_001800"
     CDS             37..471
                     /locus_tag="VVN06_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563291.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            483..1673
                     /locus_tag="VVN06_001801"
     CDS             483..1673
                     /locus_tag="VVN06_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU5563292.1"
                     /translation="MIKKVTIEEIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKENFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYKYSQQDEFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYVSANNVIRDDASSFHT
                     FYFDPETGQPLKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1684..2175
                     /locus_tag="VVN06_001802"
     CDS             1684..2175
                     /locus_tag="VVN06_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU5563293.1"
                     /translation="MTTPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELNQTYQVTFNTKTTPTGNDGAVEVNIM
                     DYI"
     gene            2190..2969
                     /locus_tag="VVN06_001803"
     CDS             2190..2969
                     /locus_tag="VVN06_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU5563294.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            2956..3774
                     /locus_tag="VVN06_001804"
     CDS             2956..3774
                     /locus_tag="VVN06_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU5563295.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKILA"
     gene            3774..4067
                     /gene="yajC"
                     /locus_tag="VVN06_001805"
     CDS             3774..4067
                     /gene="yajC"
                     /locus_tag="VVN06_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5563296.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRKEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4089..>4591
                     /locus_tag="VVN06_001806"
     CDS             4089..>4591
                     /locus_tag="VVN06_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligohyaluronate lyase"
                     /protein_id="HEU5563297.1"
                     /translation="MKEERRQFFERIDGDQYRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIDQVREFRPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEEELENIL
                     VSLEKQI"
BASE COUNT         1374 a          810 c         1031 g         1376 t
ORIGIN      
        1 agtaaaccgg taaactcaaa aaagaaagga gaaaagatga aaattgtact tgtagggcat
       61 ggacattttg ccacagggat ttatagttct ttacaattga ttgcaggcaa tcaagaaaat
      121 gtggaggcga ttgactttgt ggaaggaatg tcagcagatg aactcaagca aaaaatctta
      181 cttgcaattt caaatgaaga agaagtttta atcctaagtg atctcttggg aggatcgcca
      241 ttcaaggttt cttctaccat aatgggagaa aatccagcca aaacaatgaa tgttctctcg
      301 ggtttgaact tagccatgtt aatggaagca gtctttgcta gaatggctca tagctttgat
      361 gaggttgtta ataaatcagt agtggcggcc cagggcggag tcgtaaatgg taaagaattg
      421 ttttcaacgg atgcagagga agaggaagaa gatttcgaat cgggtattta aagggtaaaa
      481 gaatgataaa aaaggttacg attgaagaaa taaaatcgcc tgagcgcttc ttagaagtac
      541 cacttctgac gaaagaagaa gtcggccagg caatcgataa ggttattcgg cagttagaac
      601 tcaaccttga ctatttcaag gaaaatttcc cgacgccagc tacctttgat aatgtctatc
      661 caatcatgga taatacggaa tggaccaatg gtttctggac aggagaactg tggttggctt
      721 ataaatacag tcaacaggat gaatttaaaa acatcgctca taaaaatgtt ctttctttcc
      781 tggatcgtgt caataagaga gtagaattgg ctcaccatga tctcggcttc ttgtacacac
      841 cgtcttgtat ggctgaatat aagataaatg gagatggaga ggccagagag gcagccttga
      901 aagctgcaga taagttgatt gaacgctatc aagaaaaagg tggttttatt caagcttggg
      961 gagacttggg caagaaagag cattaccgtt tgattatcga ctgcttgctc aacatccaac
     1021 tcttattctt tgcttatcaa gaaacaggtg atcaaaaata ctacgatatt gcagaaagtc
     1081 atttctatgt ttcagctaat aatgtaatcc gtgatgacgc ttcgtccttc cacaccttct
     1141 actttgatcc tgagacaggt caacccctta aaggtgtaac gagacaaggg tatagtgatg
     1201 attcatgctg ggcacgtggt caatcatggg gagtctatgg tattcctttg acttatcgtc
     1261 acctgaaaga cgagtcctgc tttgacttgt ttaagggtgt aaccaattat ttcttgaatc
     1321 gtctgccaaa agatcatgtt tcttattggg atttgatttt taatgatggt agtgatcaat
     1381 cacgagattc ttcagcaaca gctatcgctg tctgtgggat tcatgaaatg ctaaaacatc
     1441 tcccagaggt ggatgctgac aaagatattt ataaacatgc tatgcatgcc atgcttcgtt
     1501 ctttgatcga acattatgca aatgatcaat ttacccctgg tgggacaagt ctcctccacg
     1561 gtgtgtactc atggcattca ggtaaaggag tggatgaagg caatatctgg ggtgactact
     1621 attacctaga agcccttatc cgtttctaca aggactggaa cctatattgg taggaggaga
     1681 aatatgacaa cgccaaatat tattatgacc cgtatcgatg aacggttgat tcatgggcaa
     1741 ggacaacttt gggtaaaata cctaggttgt aatacggtca ttgttgccaa tgacgaagta
     1801 agcacggaca agatgcaaca aactctgatg aaaacagttg tgccagactc agttgccatg
     1861 cgtttcttcc ctttgcaaaa ggtgattgat atcattcaca aggccaatcc tgctcaaacg
     1921 atctttatcg ttgtaaagga tgtgaaggac gctttaacct tggtagaagg tggtgtccct
     1981 attaaagaaa tcaatatcgg gaacattcac aatgcccctg gtaaagagca agtgacacgc
     2041 tccatcttcc tgggtgaaga ggacaaggca gccctcaagg aattgaacca aacttatcaa
     2101 gtaacattta atacgaaaac aactccaaca ggaaatgatg gagctgttga agtcaacatt
     2161 atggactata tttaaaagag gagatcgtta tgtcgattaa tgtatttcaa gcgattttaa
     2221 ttggattatg gacagctttc tgttttagtg gaatgctgtt aggaatttac accaatagat
     2281 gtattgttct gtcatttggt gtcggaatta ttctaggtga tttgcctact gctcttgcaa
     2341 tgggagctat tggtgaattg gcttatatgg gattcggtgt tggtgctgga ggtactgttc
     2401 caccaaatcc aatcggacct ggtatctttg gtaccttgat ggctatcact agtgctggta
     2461 aagtcagtcc agaagcggct cttgccctct ctactccgat tgctgtagcg attcaattct
     2521 tacaaacttt cgcctacact gtacgtgctg gtgcgcctga aacggcgatg aagcacttga
     2581 aaaaccataa tttgaagaaa ttcaagttca ctctaaatgc aacaatttgg ttgtttgcct
     2641 ttattggatt taccttgggt tgcttgggtg ccctttcaat ggataccttg ttgaaactcg
     2701 tagactacat tccaccagta ttgcttacag gtttgacagt tgctggtaaa atgctcccag
     2761 ctatcggttt tgcaatgatc ttgtcagtga tggctaagaa agagttgatt ccgtttgtct
     2821 tgttgggata tgtttgtgcg gcttatctaa acatcccaac aattggtatt gcaattgtag
     2881 gtactatctt tgctttgatt gaattttata acaagccaaa aacagcgaat catgtggtag
     2941 aggaggaagc acacgatgac tggatctaac aaattaacaa aacgtgatta tcttaaaacg
     3001 tctttgcggg cattcttttg tcaaaatgga tttaactata gtaactatca gggattagga
     3061 tatgctaatg tgatgtatcc tgctttgaaa aaacactatg gagaggatca ggaaggtttc
     3121 taccaagcct tggaagaaaa ctgtgaattc tataatacca acccacactt cctgcctttt
     3181 attaccagtt tgcaccttgt aatgttggaa aatggccgtc cggcaaaaga gacacgtagc
     3241 atcaagatgg ccttgatggg gccattggca ggtattgggg attctctctc acaattttgt
     3301 ttagctccat tgttctcaac catcgcagct tcttttgctc aagaaggcct ggttgtcggt
     3361 ccaatcttgt tcttcctagc gatgaatacg attttgacag cgattaaatt gtcaactggt
     3421 ctctatggat acaaactagg aacaactgtg attgataagc tgagcgaaca gatggcaacg
     3481 atttctcgta ttgctaatat tatcggtgta accgtaattg ctggtttggc agcgacatct
     3541 gtgaagatta tggtgccaat cacctttgct gcaggggaag ttaaagcaga cgctaaacaa
     3601 agtatcgtaa gtattcaggg aatgcttgat aaggttgctc cagctcttct accagcccta
     3661 tttacacttt tagtttacta cttgatcaaa gaaaagaaat ggacaacata taaactcgtt
     3721 attttaacag ttatcatcgg aattatcgga agctggctta agattctagc ttaatggata
     3781 caacattgtt ttatggaata gtgattgtct tggcagtgag ccccctttta ctgtcaagct
     3841 ttcattctat tcgtcagcaa aagttgcttc gcaaacagat ggagcaacga aaagagtatt
     3901 tagcttcttt aacatctggt gatgaagtgt tgttattgtc tggaattcat ggaaaaatca
     3961 tttctatcaa agatgacttg gtctccttgc agattgcaaa aggtgtggtc atctatgtag
     4021 aaaaggaaag tgtaatggga aagacaaaag aactgctttt taagtagttc tttttctggt
     4081 aatagataat gaaagaagaa agaagacaat tttttgaaag aattgatggg gaccaatatc
     4141 gtgattatat cttgtcccac tgttcaaaag actatgagaa agtcaagagt tcccttgagc
     4201 gcttgatgga caatcgtttt atgtttgata gtccttggga tatggaacct tgttcaaaaa
     4261 tccatcaaat ccagccgatg gtatgggatc aagtatttga agatgatcca gaatgggctt
     4321 atatgctcaa tcgacaagaa tatctcttgc agtttatgat agggtatctg gtagaaggag
     4381 ataagggcta tattcaaaag tgcaagttct ttctatttga ctggattgat caggtgagag
     4441 aatttcgtcc tcaatctttg atgactagaa ccttggatac gggtattcgt tcctttactt
     4501 ggttgaaact actcttgctc ctcttgaaat ttgacttgct agaggaggaa gaactagaga
     4561 acattttggt cagtctagaa aagcagattg a
//
LOCUS       DAVHIU010000122         4588 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVHIU010000122 DAVHIU010000000
VERSION     DAVHIU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.124"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            10..708
                     /locus_tag="VVN06_001807"
     CDS             10..708
                     /locus_tag="VVN06_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU5563298.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     misc_feature    773..860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            896..1210
                     /gene="rplU"
                     /locus_tag="VVN06_001808"
     CDS             896..1210
                     /gene="rplU"
                     /locus_tag="VVN06_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5563299.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1226..1570
                     /locus_tag="VVN06_001809"
     CDS             1226..1570
                     /locus_tag="VVN06_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU5563300.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1587..1880
                     /gene="rpmA"
                     /locus_tag="VVN06_001810"
     CDS             1587..1880
                     /gene="rpmA"
                     /locus_tag="VVN06_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5563301.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2085..2290)
                     /locus_tag="VVN06_001811"
                     /pseudo
     CDS             complement(2085..2290)
                     /locus_tag="VVN06_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2728..3645
                     /locus_tag="VVN06_001812"
     CDS             2728..3645
                     /locus_tag="VVN06_001812"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5563302.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3738..4586)
                     /locus_tag="VVN06_001813"
     CDS             complement(3738..4586)
                     /locus_tag="VVN06_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU5563303.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1453 a          780 c          951 g         1404 t
ORIGIN      
        1 gaaaatacaa tgggagggaa aatgaggtta ttacctataa gaaaaatatc acgtcagtct
       61 aaaaggttag cacttttttt gacgttttgt gctggatatg tggatgctta cacttttatt
      121 gttcgcggga atacccttgt agctggacaa actggaaatg ttgtctttct ttcagtagaa
      181 ttaattaaaa ataatgtttc ggatgttagg gacaaggttc tcaccttgct agcgtttatg
      241 atgggagtct ttttattaac gatttataag gaaaaattga gaattgtgaa aaaacctatt
      301 ctgtccttga ttcccttggc aatcttatca atcattattg cttttgtgcc gcaaactgtg
      361 gataatatct atctagtgcc gcccttggcc ttctgtatgg gactggtgac aactgctttt
      421 ggagaagtgt cgggtattgc ctataataac gcttttatga cagggaatat caaacggacc
      481 atgctggctt ttggagatta tttccgaacc aagcacactc cttttttgcg tgaaggattc
      541 atatttgtta gcctgcttag tagttttgtc cttggcgttg tcttttcagc ctatttgacg
      601 attttctatc atgaaaagac cattcttggt gttcctatta tgatgagcgt tttttacctc
      661 agcatgcttt ttgcctcttg gcagaaaaaa gtaaaagaaa aagcttgatt ttaggtgtta
      721 ttacttgtaa gaaaatagga gatatgctat acttgaagag ttgactatgc acgatcctgt
      781 gcaaccgcac gaacatcgtt gcttgattat tcaagtttaa gtggttcgtc ccacgatgct
      841 aggcgagtct tcacaaaaaa tcggggaaac ccataaaaat cataggaggt gcataatgag
      901 cacatacgca attatcaaaa ctggcggaaa acaagttaaa gttgaagttg gtcaagcagt
      961 ttacgttgaa aaattgaacg ttgaagctgg tcaagaagtt acttttaacg aagttgttct
     1021 tgttggtggt gaaaatactg ttgtcggaac tccacttgtt gctggagcta ctgtagttgg
     1081 aactgttgaa aaacaaggaa aacaaaagaa agtggttact tacaagtaca aacctaaaaa
     1141 aggtagccac cgtaaacaag gtcaccgtca accatataca aaagttgtca tcaacgcaat
     1201 caacgcttaa ttttaaggag aacacatgat acaggcagtc tttgagagag ccgaagatgg
     1261 cgagctgagg agtgcggaaa ttactggaca cgccgagagt ggcgaatacg gcttagatgt
     1321 cgtgtgtgca tcggtttcta cgcttgccat taactttatc aattctattg agaaatttgc
     1381 aggctatgaa ccaatcctag aattaaacga agatgaaggt ggctatctga tggttgaaat
     1441 accaaaagat cttccttcac accagagaga aatgacccag ttattctttg aatcattttt
     1501 cttaggtatg gcaaacttat cggagaacta ttctgagttc gtccaaacca gagttatcac
     1561 agaaaactaa cacggaggaa aacattatgt taaaaatgac tcttaacaac ttgcaacttt
     1621 tcgcccacaa aaaaggtgga ggttctacat caaacggacg tgattcacaa gcaaaacgtc
     1681 ttggagctaa agcagctgac ggacaaactg taacaggtgg atcaatcctt taccgtcaac
     1741 gtggtacaca catctatcca ggtgtaaacg ttggtcgtgg tggagacgat actttgttcg
     1801 ctaaagttga aggcgtagta cgctttgaac gtaaaggacg cgataaaaaa caagtttctg
     1861 tttacccaat cgctaaataa aaaggtccat tgaacctttt atcccgaacc ttgaaatgta
     1921 aaggtgagga agctagaaac agcttaaaat aaggctttcc gagttttcgg agagcctgtt
     1981 tttttgtttt ggtacccata ctttttaata tgtatagtgg attgaatctg gaatagaaca
     2041 ctgttaaaaa ttgatatgag tttcctactc gatttcttca gattttattt caattgacta
     2101 taagaactgc tatctggttt caaatattgg cttgtcgtcc cttcacttgt ctgaatggtt
     2161 tgagccgttc cttctgataa gggaaaagat attctttagc tgaaactgtc tgagaatcca
     2221 agctaacaga tggagagaaa aatagcccag tctgaagcac actagatggg agttgttgga
     2281 aagcagtcat ctagtttttc tttgctgttt tggctttttt ctttgtcatc agggaaagtg
     2341 acttgcttca aatcagggag tttaggaaat tcttcaaaca tcttgcccaa gttgtctcat
     2401 tttgtaaatt ttacagccat aggtgtacct cgattctaaa ttctaccttt tcggcaccaa
     2461 ttagtaattt aaagttctca ggagttagtt gcattgcttt ttcatacata agcatagtat
     2521 gacactttat ggttaccgaa caagtctaat cactccttgc aaaattttga agatgtccta
     2581 caagtttttg agaagaatat tgcaaagttc atagttaagc aacgatagaa taaagtgctt
     2641 tttgaaaaca ggtacacttc atgtgaaaaa aacaacattt cagattttct gttccagaaa
     2701 tagctatcgc tatgatataa ttattttatg attattacta ttcctataaa aaaccaaaaa
     2761 gatattggca caccatctga ttcagtcgtt gttctcggct attttgatgg catacataag
     2821 gggcatcaag aattatttcg tgttgccaac aaggctgcga gaaaggattt attacctatc
     2881 gtcgttatga cctttaatga atctccaaag atcgctttag agccttatca tccagatttg
     2941 tttttgcata ttttgaaccc tgctgaacgt gaaagaaaat taaagcgcga aggtgtagaa
     3001 gaattatatc tccttgattt tagtagtcaa ttcgctagtc tcactgcaca agaattcttt
     3061 gcaacttata tcaaggctat gaatgccaaa attattgttg caggttttga ttatacattt
     3121 ggttctgaca aaaaaacagc agaagattta aaggattact ttgatggaga agttatcatt
     3181 gttccacctg tagaagatga gaaaggaaag attagttcaa ctcgtatccg tcaagctatt
     3241 ttagatggaa atgtgaaaga agcaggaaaa cttttggggg caccgcttcc atcaagaggt
     3301 atggtggttc atggtaatgc tcgtggtcgt acaattggtt atccgacagc gaatttagtg
     3361 cttttagacc gtacttatat gccagcagat ggcgtttatg tcgttgatgt tgagattcaa
     3421 agacagaagt atcgtgctat ggctagtgtc gggaaaaatg tgacctttga tggagaagaa
     3481 gcacgttttg aagtcaatat ttttgatttt aatcaagata tttatgggga aaccgtcatg
     3541 gtttattggc ttgatcgcat tcgtgatatg accaaatttg actcagttga ccaattagtg
     3601 gatcagttaa aggctgatga agaagtaact cggaattggt cttaagagct tgagtaaata
     3661 aaataaaaaa gaggttgtct gtaacccaaa agatagatga tttagtctaa cttttgaggt
     3721 cacgacatta cctcttttta ttctttttca aaggtgaagc cttctcctag gatttcatgg
     3781 gcttctgtaa tagttataaa ggcttgagga tcgattcgat gaatcatttc cttcgttttc
     3841 acaatttcat ttcttccgac aatacagtag atgattttca aattttcttt actatagtag
     3901 ccttgaccag aaataaaagt aacacctctt ccgaggtcat cattaatcgc cttagcaagt
     3961 tggtcaggac gttttgtgat aatcataaag cctttgccgg catatcctcc ttcaccaatc
     4021 aaatcaataa cacgagaaac aataaaatca aacaaaagcg tgtaggaaac caatctcaaa
     4081 tccttgaaga ttaggagaat caacatgaga atacaaaaat ctaagataaa gagcagtttt
     4141 cctatggata tatgagtgta tttgttgaga atacgagcta gaatatcagt tccgccagtt
     4201 gtacctccag cattaaaaat aattccaagg ccaattccca ataggattcc cgctataagg
     4261 gctgtgatta gtaaatcacc ttgaagatca atatgaaggg gaatatgctc aaaaaaagct
     4321 aaccaggcgg acaaagctaa ggttcctagt aaactagaat agagggattt ggctccaaag
     4381 atcttccaag ctaggatgaa aaggggaata ttaatcagca ggttcatgag ggaaacaggg
     4441 attttaaaaa gataaaaggt gatgagggta atgcctgtcg cccctccttc aaagagatga
     4501 tggggaacta caaaataagt cagtccaaaa gcataaatag cagcacctag taaaatggta
     4561 aaaatggggt aaattttttt aatcatag
//
LOCUS       DAVHIU010000123         4583 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVHIU010000123 DAVHIU010000000
VERSION     DAVHIU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.125"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            8..1822
                     /locus_tag="VVN06_001814"
     CDS             8..1822
                     /locus_tag="VVN06_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5563304.1"
                     /translation="MEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYI
                     IYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFS
                     IRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQ
                     HPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSE
                     YERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGA
                     ELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDY
                     VPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKA
                     VDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSR
                     GSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKP
                     VKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKV
                     NVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1718..2212)
                     /locus_tag="VVN06_001815"
     CDS             complement(1718..2212)
                     /locus_tag="VVN06_001815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563305.1"
                     /translation="MKIKEQTRKVTAGCSKHCFKVVDGTDEVSSKHCFEVADRTDEVS
                     SKHCFEVADRTDEVSSKHCFEVADRTDEVSSKHCFEVADRTDEVSNHIYGKATLTWFE
                     EIFEEYKSLHNKTHSTKVNKLDTMRFSFLFNVGCLISKGDNSLLQLISTESAELSNLL
                     IDGF"
     gene            complement(2262..2894)
                     /locus_tag="VVN06_001816"
     CDS             complement(2262..2894)
                     /locus_tag="VVN06_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5563306.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIVELLQELSKNYPL
                     YITTTKNTPTAQDMTKNLGIHHFFDGIYGYSPEAPHKADVIHQALQIHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLEYFQ"
     gene            complement(2916..3788)
                     /gene="sdaAA"
                     /locus_tag="VVN06_001817"
     CDS             complement(2916..3788)
                     /gene="sdaAA"
                     /locus_tag="VVN06_001817"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU5563307.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3797..4468)
                     /gene="sdaAB"
                     /locus_tag="VVN06_001818"
     CDS             complement(3797..4468)
                     /gene="sdaAB"
                     /locus_tag="VVN06_001818"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU5563308.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
BASE COUNT         1228 a          934 c         1031 g         1390 t
ORIGIN      
        1 tgatctggtg gaaattttaa ataaacaaca aaagcaggtt ttctggggaa tatttgatat
       61 tttcagtatg gtggtttcca tcattgtatc ttatatttta ttttatgggc tgattaatcc
      121 agcacctgtt gactacatta tctatacgag tttggccttc ctgttctatc aattgatgat
      181 tggtttttgg gggttgaacg cgagcattag tcgttacagc aagattacgg atttcatgaa
      241 aatctttttt ggtgtgactg ctagcagtgt cttgtcatat agtatctgtt atgccttctt
      301 gccactcttc tccatccgtt ttatcattct ctttatcttg ttgagtacct tcttgatttt
      361 attgccacgg attacttggc agttaatcta ctccagacgc aaaaaaggta gtggtgatgg
      421 agaacaccgt cggaccttct tgattggtgc cggtgatggt ggggctctct ttatggatag
      481 ttaccaacat ccaaccagtg aattagaact ggtcggtatt ttggataagg attctaagaa
      541 aaagggtcaa aaacttggtg gtattcctgt tttgggctct tatgacaatc tgcctgaatt
      601 agccaaacgc catcaaatcg agcgtgtcat cgttgcgatt ccgtcgctgg atccgtcaga
      661 atatgagcgt atcttgcaga tgtgtaataa gctgggtgtc aaatgttaca agatgcctaa
      721 ggttgaaact gttgttcagg gccttcacca agcaggtact ggcttccaaa aaattgatat
      781 tacggacctt ttgggtcgtc aggaaatccg tcttgacgaa tcgcgtctgg gtgcagaact
      841 gacaggtaag accatcttag tcacaggagc tggaggttca atcggttctg aaatctgtcg
      901 tcaagttagt cgcttcaatc ctgaacgcat tgtcttgctc ggtcatgggg aaaactcaat
      961 ctaccttgtt tatcatgaat tgattcgtaa gttccaaggg attgattatg tacctgtgat
     1021 tgcggacatt caagactatg atcgtttgtt gcaagtcttt gagcagtaca aacctgctat
     1081 tgtttatcat gcggcagccc acaagcatgt tcctatgatg gagcgcaatc caaaagaagc
     1141 cttcaaaaac aatatccgtg gaacttacaa tgttgctaag gctgttgatg aagctaaagt
     1201 gtctaagatg gttatgattt cgacagataa ggcagtcaat ccaccaaatg ttatgggagc
     1261 aaccaagcgc gtggcggagt tgattgtcac tggctttaac caacgtagcc aatcaaccta
     1321 ctgtgcagtt cgttttggga atgttcttgg tagccgtggt agtgtcattc cagtctttga
     1381 acgtcagatt gctgaaggtg ggcctgtaac ggtgacagac ttccgcatga cccgttactt
     1441 tatgaccatt ccagaagcta gccgtctggt tatccatgct ggtgcttatg ccaaagatgg
     1501 ggaagtcttt atccttgata tgggcaaacc agtcaagatt tatgacttgg ccaagaagat
     1561 ggtgcttcta agtggccaca ctgaaagtga aattccaatc gttgaagttg gaatccgccc
     1621 aggtgaaaaa ctctacgaag aactcttggt atcaaccgaa ctcgttgaca atcaagttat
     1681 ggataagatt ttcgttggta aggttaatgt catgccttta gaatccatca atcaaaagat
     1741 tggagagttc cgcactctca gtggagatga gttgaagcaa gctattatcg cctttgctaa
     1801 tcaaacaacc cacattgaat aaaaaagaaa aacgcatagt atcaagtttg ttaaccttgg
     1861 tactatgcgt tttattatgt agagacttat actcttcgaa aatctcttca aaccacgtca
     1921 acgtcgcctt gccgtagata tggttactga cttcgtcagt tctatctgcc acctcaaaac
     1981 agtgttttga gctgacttcg tcagttctat ctgccacctc aaaacagtgt tttgagctga
     2041 cttcgtcagt tctatctgcc acctcaaaac agtgttttga gctgacttcg tcagttctat
     2101 ctgccacctc aaaacagtgt tttgagctga cttcgtcagt tccatctaca accttaaaac
     2161 agtgttttga gcagcctgcg gttactttcc tggtttgctc tttgattttc attgagtatt
     2221 acttcatttt cttctgaaat ggagttgttg cctagtctat gttattgaaa atactccaaa
     2281 acttctaagg gtttgtgagc gatataatca ggttgatagt ttagtagatc tgcttgctct
     2341 ccaaatcccc aagtgatggc caatttctga atacctgttt ctcgagctcc cagcatatca
     2401 aacttggtat ctccgatgat gatggcttgt tctggtgcta gttgatgtat ctgcaaggct
     2461 tggtgaatga catctgcctt atggggtgct tcagggctgt aaccataaat gccatcaaag
     2521 aaatgatgga ttcccaagtt ttttgtcatg tcttgagcgg taggtgtatt ctttgtagtg
     2581 gtgatgtaga gagggtaatt cttggatagt tcttgaagta attctacaat ctgaggaaag
     2641 agttgagctt catagatgcc ttttgcctta tagtaagaac gatatatctg cacggcttca
     2701 gaaatttggt ctttggacag gcaggtcgca aaactacttt cgagaggtgg tcccataaaa
     2761 ccacgaatag ttttggcatc agggctaggc acccccagct ctttaaaggt ataggtaaag
     2821 gcattgtgaa tcccgataga actatcaacg agggttccat ccaaatcgaa aaaaatcgct
     2881 gtgatagagg tcatggtttc tcctatttga taagcttatt ctccgaaaat ttctttttgg
     2941 aggcgacgac cagtaggggt ggtagcgagt ccaccttcag ctgtttcacg aaaggcagtt
     3001 ggcatgcttg ctcctacttg gtacatggca tcgatcactt catccacagg gattttagat
     3061 tcgatacctg ccaaggccat gtctgctgcg atgaaagcaa agctagctcc catggcatta
     3121 cgtttgacac agggaacttc gaccaaacct gcaacagggt cacagatgag gcctagcata
     3181 tttttaatga caaaggcaat agcttgactg gcctgataag gtgttccacc tgcagccaga
     3241 gtcaaggcgg cagcactcat agcagaggct gaaccaactt cagcttgaca cccaccctca
     3301 gcacctgaga tggaggcatt gtttgcgatg actagtccaa aggcaccagc agcaaagagg
     3361 aaatccaatt gttgctcgtg gctgaggtct aatttttcaa tagcagcagt gagaacggat
     3421 ggcagacagc cagcacttcc agcggttgga gtggcacaga ccaagcccat tttggcattg
     3481 tgttcattga ctgcgatggc atttcgggca gccgagagaa tcgtataatc tgacagagtt
     3541 tttccgtttt cgatgtagtg atccaatttg gcagcatctc cacctgtcag gccactacga
     3601 gatttatttt cattgaggcc aagttggaca gaggctttca taacttccag attgcgttcc
     3661 atgagaagga agacttcttc acgttcgcga ccggtcaatt caaactctgt tgtaatcatg
     3721 agttctgcga catttccttg aaagtctaga tctgcttgct cgaccaattc tttgatagaa
     3781 taaaacatgc ttcctcctat ttaaagaaat tgacattgtg gagatgaggg atttttcgaa
     3841 tttcttcgat agcctcatca cagttgcgac tgtcaacttc gataatcata atggcttttt
     3901 caccagcttt ttcacgagtg acattcatct gggcgatatt gataccatag cgggaaagcg
     3961 cctctgtaac aagggcaatc atacctggaa tatcttgatg aacgatgatg atagtcggtg
     4021 tattcatatt gagagagacg gcaaaaccat tgagttcggt tacctgaata tttcctccac
     4081 cgatagaaat accagtcacg ctgatggtct tgtgggcatt tttaacagta attttagtgg
     4141 tgttagggtg aggggcattg ctgtctttct gaatggtcca gacaatcttg ataccacgct
     4201 tgtgggcaat ttccagacta tttggaattt caggatcatc tgtatccatt cccaaaatac
     4261 ctgcaacaag ggctaggtct gttccgtgac cacgataggt cttggcaaat gagttaaaaa
     4321 gttggaattc aacttctgtc ggagtatcat caaaaatgga agagacaatc ttcccaatac
     4381 gaacagcacc agcggtatgg ctactagatg ggccaatcat aactggtccg atgatatcaa
     4441 agacagattg aaaacgaagt gatttcatca gtttcccctt ataaaaattc ttatctctat
     4501 tatatcaaag aatgaggggc ttggctttaa ttgtggatga aaacctttct aatacctcaa
     4561 atagcataaa aatagtatct ttt
//
LOCUS       DAVHIU010000124         4574 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVHIU010000124 DAVHIU010000000
VERSION     DAVHIU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.126"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(143..1129)
                     /locus_tag="VVN06_001819"
     CDS             complement(143..1129)
                     /locus_tag="VVN06_001819"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU5563309.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1323..3791
                     /gene="gyrA"
                     /locus_tag="VVN06_001820"
     CDS             1323..3791
                     /gene="gyrA"
                     /locus_tag="VVN06_001820"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5563310.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3791..4534
                     /gene="srtA"
                     /locus_tag="VVN06_001821"
     CDS             3791..4534
                     /gene="srtA"
                     /locus_tag="VVN06_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU5563311.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         1399 a          805 c         1025 g         1345 t
ORIGIN      
        1 taatggattg aaataagata tgaacaaatc gattaggaat ttaaagcatt ttataacaac
       61 gttttagagt aacggggtac tatttcatat aatacaggaa aaaacaactc cctgatgatt
      121 caaggagttg tctatagtta aattagtttt tagaagcttc ttggaattct ggatttttcc
      181 atgcttcgtc aataatagct tgcaattctt tagcagatgc ttgcattttt tgagtttctg
      241 catcgttcaa tggaatgttt actggacgaa cgataccatg tgcaccaaca acagctggtt
      301 gaccgataaa gacattctca actccgtatt gaccttcttg gaatactgaa agtggaagta
      361 ctgcgttttc atcgtcaagg attgctttag tgatacgagc aagggctact gcgataccgt
      421 agtatgttgc accttttttg ttgatgattg tgtaggctgc atcacgaaca ccttcgaaca
      481 attcaatcaa ttcagcttct tgaacatttt gagtgtcttt aagaaattct tcaaggttta
      541 caccagcgat gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt tcacccatga
      601 tgtaggcgtg cactgaacga gcatccacat ccaatttttc agcaagtgct tgacggaaac
      661 gagctgagtc aagtgaagta cctgaaccga taacgcgttc tttagggaaa ccagagaatt
      721 tccaagttga gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag atacctttga
      781 aaccagattc aacaacttga gttacgattg atttgttgat agcaaggttt ttacctacaa
      841 ggtcaagacg agtttcacct ggtttttgag gtgcacctgc agtgatcaca acaaggtcag
      901 cgtctgcaca gtcagagtat tgagctgcat agattttttt aggtgaagtg aaggcaaggg
      961 cgtgactaag gtcaagcgca tcaccaacag ctttttcatg caattgtgga atttcgataa
     1021 ttccaagctc ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat gaacctacag
     1081 caccatcacc gacaaggata acttttttgt gttgtttagt tgaagtcatt gttttaaaca
     1141 tctccttaat tttattaggg gattttccct agacaacttc attctatcac ttttaaaaaa
     1201 ctttgtcacg aatatgcctt atagttctcg atgtaaacgt tttagtggtt tagaggctga
     1261 aatagatggg aatttatggt ataatgttgt tacttactaa ttgtgaaatg aggcatttat
     1321 taatgcagga taaaaattta gtgaatgtca atctgacaaa ggagatgaag gcaagtttta
     1381 tcgactacgc catgagtgtt atcgtagcgc gagctcttcc tgatgttcga gatggcttaa
     1441 aacctgttca ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc ccagacaaac
     1501 cccataaaaa atctgctcgt attacagggg atgtcatggg taaatatcac ccacacgggg
     1561 attcctctat ttatgaagcc atggtccgta tggctcaatg gtggagctac cgttacatgc
     1621 ttgtagatgg tcatgggaat tttggttcca tggatggaga tagtgctgcc gctcaacgtt
     1681 ataccgaggc acgtatgagc aagattgctc tggaaatgct tcgtgatatc aacaaaaata
     1741 cagttgattt cgttgataac tatgatgcca atgaacggga acccttggtc ttgccagcgc
     1801 gttttccaaa ccttttggtt aatggagcaa ctggtatcgc ggttgggatg gcaaccaata
     1861 ttccacctca taatctgggt gaaaccattg atgcagtgaa gttggtcatg gataatcctg
     1921 aagtgactac caaggacttg atggaagtct tgcctggacc agattttcca actggtgctc
     1981 ttgtcatggg gaaatcaggt atccataagg cttatgaaac aggtaaaggt tcgattgtcc
     2041 tacgttctcg tacagagatt gaaacgacta agactggtcg tgagcgtatc gttgtaacag
     2101 aatttcctta catggtcaat aaaaccaagg tgcatgagca tattgttcgc ttggttcagg
     2161 aaaaacgcat tgagggtatc acagcagtac gtgatgagtc aaaccgtgaa ggtgttcgat
     2221 ttgttattga agtcaagcgc gacgcctcag ccaatgttat tctcaataac ctcttcaaaa
     2281 tgacccaaat gcaaaccaat tttggtttca atatgctcgc tatccaaaat ggtataccga
     2341 aaattttgtc tcttcgtcag attttggatg cttatatcga gcaccaaaaa gaagtggttg
     2401 ttcgtcgtac acgttttgat aaggaaaaag cggaagcgcg cgctcatatc ttagaaggtc
     2461 tcttgattgc gctagaccat atcgacgaag tgattcgtat catccgtgct agtgaaacgg
     2521 atgcggaagc tcaagctgag ttgatgagca agtttaagct ttctgaacgt caaagtcaag
     2581 ctatccttga tatgcgtctt cgtcgtttga caggtttgga acgcgataag attcaatctg
     2641 agtatgatga cctcttggct ctgattgcgg atttagcaga tattcttgct aagcctgaac
     2701 gtgtttctca aattatcaaa gacgaattgg atgaagttaa acgtaaattt tctgataagc
     2761 gccgtacaga gttgatgatt ggacaggtct tgagtctcga ggatgaggac ttgattgaag
     2821 aatcggatgt cttgattacc ctttctaaca gaggctacat taagcgtttg gatcaggacg
     2881 agttcactgc tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg aaagatgatg
     2941 actttgttcg tgagttagtg tcaactagca cccatgatca tctgctcttc ttcacaaaca
     3001 agggacgtgt ctatcgtctt aaaggttatg aaattcctga gtatggtcgg actgccaaag
     3061 ggctaccagt agtcaatctc ttgaaattgg atgaagacga aagtattcag acggttatca
     3121 atgttgagtc tgatcgcagt gatgatgctt atctcttctt tacaacccgt cacggtattg
     3181 tgaagagaac cagtgttaag gagtttgcca atattcgtca aaatggtctc aaagcgctga
     3241 atttaaagga tgaagatgag ttaatcaatg tcttgttggc agaaggagat atggatatta
     3301 tcattggtac caagtttggt tatgcagttc gctttaatca atcagccgtt cgtggtatga
     3361 gtcgtatcgc cactggtgtg aaaggtgtta accttcgtga aggagacaca gttgttggtg
     3421 ccagcttgat tactgatcaa gatgaggttc ttattatcac agaaaaagga tatggtaagc
     3481 gtacagtcgc tactgaatac ccaacaaaag gtcgtggtgg taagggaatg cagacagcta
     3541 aaattaccga aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg gatgaggatt
     3601 tgatgattat cactgataca ggtgtcatga ttcgaaccaa tcttgccaat atttcacaaa
     3661 caggacgtgc aactatggga gttaaagtaa tgcgcctgga tcaagatgct cagatagtga
     3721 ctttcacaac ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac gaaacagaag
     3781 gtgaagcata atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa atctgctgac
     3841 aaatatccta gcaggatttc tgatattact gtcactggct ttgattttta atactcaaat
     3901 tcgaaatatt ttcatagtct ggaataccaa taagtatcaa gttagccagg tatcaaaaga
     3961 aaaattagaa gaaaatcagg atacagaagg caattttgac tttgattctg tcaaagctat
     4021 ctcttcggaa gctgttctaa cttctcaatg ggatgctcaa aaattaccag ttattggggg
     4081 aattgcaatt cctgaattgg aaatgaattt gccgattttt aaaggacttg ataatgttaa
     4141 tctcttctac ggagctggta caatgaaacg cgagcaagta atgggagaag gaaattatag
     4201 tctagctagt caccatatct ttggtgttga taatgctaat aaaatgttat tttctccttt
     4261 agataatgct aaaaatggca tgaagattta tctaaccgat aaaaataaag tttatactta
     4321 tgaaatacgt gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg atgatagaga
     4381 tggggtcaat gaaatcacat tagtaacctg tgaagacctt gctgctacag aacgtattat
     4441 tgtcaaaggt gatttgaaag aaacaaaaga ttattcacaa acatctaatg aaatcctaac
     4501 agctttcaat caaccatata aacaatttta ttaatacaaa tcagtgaaat ccatgatttc
     4561 actgattttt taag
//
LOCUS       DAVHIU010000125         4567 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVHIU010000125 DAVHIU010000000
VERSION     DAVHIU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.127"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(352..903)
                     /locus_tag="VVN06_001822"
     CDS             complement(352..903)
                     /locus_tag="VVN06_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563312.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1253..2077)
                     /gene="nadE"
                     /locus_tag="VVN06_001823"
     CDS             complement(1253..2077)
                     /gene="nadE"
                     /locus_tag="VVN06_001823"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU5563313.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2074..3534)
                     /locus_tag="VVN06_001824"
     CDS             complement(2074..3534)
                     /locus_tag="VVN06_001824"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5563314.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMHNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3650..4138)
                     /locus_tag="VVN06_001825"
     CDS             complement(3650..4138)
                     /locus_tag="VVN06_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563315.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4128..4337)
                     /locus_tag="VVN06_001826"
     CDS             complement(4128..4337)
                     /locus_tag="VVN06_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5563316.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1309 a         1056 c          840 g         1362 t
ORIGIN      
        1 aatgagacta ttttaatatt agatattttg atagaaaaaa acataaataa aattggttga
       61 atttaattcc ctaactcgat agaggattct attttaaatt taacaacagc taggattaca
      121 ctataaaaag cacattacca cctgttcggc tggagaatct agtcgattag ctccctctta
      181 gttacaagct tcaagcgatt ttttaactaa tactaaacca gattaaagaa gaaactagaa
      241 catctgtctt gtactaatca atatgctatt ttaaagttgc aaataaactt ggaatgctaa
      301 ttattatatt tacaataaat agttgacttc gatagaaaca gaaaatagaa ttcaacattc
      361 ttccctactt ataccataat aaataagatc cgcaagatag ccttttctct ctacaccatt
      421 aacaatggtt tgtagatagg acattccagc cttttccatg acacgacctg aagctgggtt
      481 gagactggca taacgtgctc tgaccttttg aaaacctgct tgagtaaaac aaaagtccaa
      541 gacagctttc aaggcctctg tcatcatacc atgtccccag taagccttgc ctagcacata
      601 gccaatttca cagcttaaat cagcctcgtc tatcttaaca atgctgatat ctcctatcac
      661 ttgctctggg ttttctttta gacaaatggc ccatttgtaa tagttgagat tagtatagga
      721 agcaacccaa ttgcgaatcg agtttcgagt gatttcgaca tcaggatggg gatcccaggt
      781 aacataggtc aaattctcag cggatgaagc ccaattttga aacatggctt ctgcatcact
      841 ctccacaaat cttcttaaaa tcaaacgttc tgtctgtaat atttgcgtac cgatagattt
      901 catgatgcta cctcacttcc ttatttctat gctcgtagta aaaagactca actcagtaac
      961 agctagaaaa cgagtttgtc ttctaatttc ttgacgcagt cgctgcctgg tttgaaatgc
     1021 gctctagcaa gctttttcaa acctagtcat cgttgcaggg gtaagacgat gaaatcgaac
     1081 tcttcgagtt actgaatctg cctcattttc ttatttctaa actcggggtt caagatcccc
     1141 cagaccttct ctcactttct tatttcaaga tgagaggctg aaaacctcca gtggaggttt
     1201 ttcttgcttt ttaatttcag ggtaagaagc taaaaaggtc ccccggacct ttttactccc
     1261 aaaagtcatc aaatacggtg atgggtaagt ggcgtttgtg ttggcctttg tgccaccagt
     1321 tttcaatggt cgcttgagct tctgggctga ttgttttgcc ttctaggtag tcgtcaatct
     1381 ctgcgtaggt gactccaagt gcgacttcgt cagctaggcc tggtttatct tcttctaggt
     1441 ctgccgttgg gattttttca taaagggctg gctctgcgcc aagtttctgc aagagctgtt
     1501 ttccttggcg tttattgagg cggtaaagag ggagaatatc cgcaccgccg tcaccaaact
     1561 tggtaaagaa acctgtgata ttttccgcgg cgtggtctgt tccaatgacc gctccgctat
     1621 gggaaccagc aagggcatac tgagcaatca tacggcaacg tgccttgata ttccccttgt
     1681 tgaagtctga aacaggacta cctgtcgctt caactgcagc tgtcatggca tcagctgatt
     1741 ccttgatatt cacaaccaag ctgacatctg gctggatgaa ggctagggct ttttgagcat
     1801 ctgcttcatc agcttgcact ccgtatggca ggcggacagc gataaatttg tagctatcgt
     1861 ctcccgtttc agctcgcagt tcttccatag ctaattgcgc caaacgtcct gccaaggttg
     1921 agtcttgtcc cccagaaatc cctagtacaa aggtttttag gaagggatgt tttttcagat
     1981 atctttttaa gaaatcaata gaacgacgga tttcttcctg ggcatcaatc actggtttga
     2041 cacccagctc ttggataatc gtttcttgca aactcattct tcttctcctt caccaagggc
     2101 ttccttgcgc atcttatcaa tcaagtccat cttatcttgc catacatcac gcgccaaatc
     2161 cactggatag tgctgcggat tgagcacacg cttatactca tcccacaact tgtcaaattc
     2221 cttacgggca taatcctgaa tgtcagtcaa actaggcaag ttgtaaacta atattccttc
     2281 tttgaagata tccaccaaga gaggaacggc atcaaaatta cgaaccgtct tcttgatgta
     2341 tgtataggtc ggatggaaca tcttgatttc tgtcatgtcg ctaatatcca caccatcata
     2401 agtgatgtag tcaccttctg acttgccttt ttcacgactg gtaatgcgcc acacctgctt
     2461 cttacctggc gtcgacactt tttccgcatt attagacagc ttaatcgtat tgtgcatctg
     2521 accagtttca tcttcaattg caacaatctt gtaaaccgcc ccaagagccg gctggtcata
     2581 ggctgtaatc agcttggtac ccacacccca gacatcaatc ttggcctttt gcatcttgag
     2641 gttaaggatg gtattttcat ctagatcatt agaagcataa atcttagcct ctgtaaatcc
     2701 agcctcgtcc agttgctgac ggactttctt agaaatgtag gcaatatccc cagagtcaat
     2761 ccgcacaccc ataaagttaa tctgatcacc cagctcacgc gccacctgaa tggcagctgg
     2821 tacaccgatg cgaagggtat cataggtatc cacaagaaag acacaatttt tgtgggtcgc
     2881 agcgtaagcc ttgaaagcct catagtcatt gccataaacc tgtaccaagg catgggcatg
     2941 ggttcccaaa acaggaatgt caaagagctt acccgcacgc acgttgctgg ttccattggc
     3001 gccaccaatc accgctgcgc gtgttcccca gatggccgca tccatttctt gagcccgacg
     3061 tgtcccaaac tccatcaagg gttcatcttc gataaccgaa cgaatacgag ctgccttcgt
     3121 cgccaccaag gtctggtagt tgacgatgtt caaaagagcc gtttcgacca actgacattg
     3181 ggctagaggt ccttccacct gcacaatcgg ttcattagca aaaaccaaat ccccttcttg
     3241 ggcagaacga acggtcaact ccaacttgaa attgcgaagg taatccaaga acgccccatg
     3301 ataaccaagc gactccaaat aggctatatc actatctgaa aaacgcaagt cttcaagata
     3361 gttcacaatt ctttccaaac ctgcaaaaac cgcatagccg ttcttaaaag gctgttggcg
     3421 gaaatacacc tcaaagaccg ccttcttatt gtgaatccct tggtcaaagt aaacctgcat
     3481 catgttgatc tggtacaagt ccgtgtgcaa tgtcaaacta tcatctggat acatactttt
     3541 cctacttcct tagctagaaa cccatgaaaa ttttcaagaa ctttcatgta ttccaataaa
     3601 ttagtactat tatatcacat tttagctgga ttgagaaaag agtaacaagc tattctccac
     3661 tctccaattc atccatatct tgttcaaatt ttttctgagc ccattcgcca tagctcttaa
     3721 gaccaagatt gccaataaag acccacggaa ggtaaatgac ataagtaatg acccaagcag
     3781 acaggtattt aaaattcaaa ggattgtgct gataaatttc tatgttgaat tgataattct
     3841 gcaacatcaa aagagccgta atagccaagg ttaggaaaaa acaacccaaa atcgtaaaat
     3901 gaaaacgact atagtaggtc actcccagat aacgggcacg gttaaaaaag taaaatgtcc
     3961 ctatgatgat aacgattagc agcatattag aattaaaaag gcttggtgct aatactgaaa
     4021 tgatataaga taggagcgac aaagcgatgc agatatagaa actttcagag cccgctttat
     4081 tgatcagttg ttcttctctt tcgtctagta attgataata ataaaatcta tttttcatct
     4141 tcttcctccc aaaatagttg gtctagggtt ttccctaaac atctgcaaat agactggcag
     4201 agcgagagac tgggattgta ttttcccgcc tctatcaaac caatagtctg gcgtgtcacc
     4261 ccgacagcct ctgccagttg accttgtgtt aaatcacgct ctacccgagc taattttaat
     4321 tttaaatttt tagccacctt cgtcctcctt atagttttaa tactcatcta ctcttaaaaa
     4381 atccaaaacc aacacaagct atcagctatt tattataacc ccattaattt gcactccagt
     4441 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
     4501 ttttattttt atttagtatt agctttagta ggatctttta aacgagtttt tattgcttat
     4561 atttctt
//
LOCUS       DAVHIU010000126         4546 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVHIU010000126 DAVHIU010000000
VERSION     DAVHIU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.128"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            154..351
                     /locus_tag="VVN06_001827"
     CDS             154..351
                     /locus_tag="VVN06_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563317.1"
                     /translation="MAMNICQRVIRLEETVERISEALDGFIENANHNAIVSNKNIERI
                     QESFEIVFKELDELKAVPDKH"
     gene            444..968
                     /locus_tag="VVN06_001828"
     CDS             444..968
                     /locus_tag="VVN06_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563318.1"
                     /translation="MTLKTFSDKAKTFNFTYEFKDQDTAQVAGSALMGYMIGTYVVPS
                     ISITYKNKGTLVAEYVEDHKLNKTFKRIRDGFKDYYKQPVNDEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELKLLDYAERLLSDKPILMDSMTAFGTLEMLGNESINLFQKLD
                     VEGEYKGLADYSGQ"
     gene            1031..1297
                     /locus_tag="VVN06_001829"
     CDS             1031..1297
                     /locus_tag="VVN06_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563319.1"
                     /translation="MVRSLKARLEKLEEYAGKTEYATTDECISLYVDCLRFAFQQDEI
                     KIDMDDFIKTPFKDIKLADYKKSKKRMDELREMTGISEFSELEQ"
     gene            1348..1689
                     /locus_tag="VVN06_001830"
     CDS             1348..1689
                     /locus_tag="VVN06_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU5563320.1"
                     /translation="MANYLMTGNVKKASELSEITRKTAYNYLNDATFKRIYRERRSEQ
                     LKESTTLLQNASVEAVNVLREIMLDKTVSPYARQQSAQSILNMAYKAVEMVEVVEQLE
                     ILEAKILDEGD"
     gene            1676..1927
                     /locus_tag="VVN06_001831"
     CDS             1676..1927
                     /locus_tag="VVN06_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563321.1"
                     /translation="MKAIKNRLNKLNKLAVVDEVDTVFLTQIDTGHFEVRYRIGGKNP
                     KDIIEVYPTKEEAEKAISEIKNTKGLRIFWDNLALLEDN"
     gene            2546..2965
                     /locus_tag="VVN06_001832"
     CDS             2546..2965
                     /locus_tag="VVN06_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU5563322.1"
                     /translation="MTPEQVKETLEGVKWINKEIEGLYLELATLESGIIKKQELSNTR
                     VQTSRVNTAENNLISVLKLKEDTLQRIERLTEERMEISRLIDKLANPLERSVLRLFYL
                     NVLEAWQVAEEIDRSKTTVYLIRQKAIEHLAGLVHAD"
     gene            complement(3416..3610)
                     /locus_tag="VVN06_001833"
     CDS             complement(3416..3610)
                     /locus_tag="VVN06_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU5563323.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(3675..>4546)
                     /gene="dnaN"
                     /locus_tag="VVN06_001834"
     CDS             complement(3675..>4546)
                     /gene="dnaN"
                     /locus_tag="VVN06_001834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5563324.1"
                     /translation="PDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISAST
                     PLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLT
                     LEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFYTRLLEGNY
                     PDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPE
                     VGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTD
                     EDFMQLITPVRTN"
BASE COUNT         1563 a          777 c          922 g         1284 t
ORIGIN      
        1 aaataatata tatacgtgtt atataggcgc gaacttaaaa agtaaaacta accctaatct
       61 aacacccttg aaacgttgat atagtaggct tttgaagggt tattttttaa acctaatcaa
      121 aacctaatct aagagaaaaa aggagcaaac cagatggcga tgaacatttg tcaaagagtg
      181 attagactag aagaaaccgt tgagcggatc agtgaggcgc tggatggctt tattgagaat
      241 gcgaaccata acgctatagt aagcaataaa aacatcgaac ggattcagga aagttttgaa
      301 atagtcttta aagagcttga tgaactaaaa gcagtacctg acaaacacta aaacatttaa
      361 acaaaatggg gtagatagta ccgaccccct tgcaatggtg taactactag tgacacccta
      421 aaaacagaaa gaggaaaaac aacatgacat taaaaacatt ttcagataaa gcaaaaacat
      481 ttaatttcac ttacgaattt aaagaccaag acaccgccca agtggcaggg agtgcattga
      541 tgggctacat gattggaact tatgtagtac cgtctatttc aatcacttac aagaataaag
      601 ggacgctagt agctgagtac gtggaggatc acaagctaaa caagaccttc aaacgtatcc
      661 gtgacggttt caaagactat tacaagcaac cagtgaacga tgaagcattt gaagaacgtt
      721 ataaacggga gcgcgtgctt caactcaaag agtcagagga ctttgagagc ttgctgaata
      781 aggtcacaga ctacgagctg aaactattag actatgctga acgcttgctt tctgataagc
      841 ctatccttat ggactctatg actgcttttg gtacgttgga aatgctgggt aatgaaagta
      901 ttaacctatt ccaaaaattg gacgtagagg gcgaatataa aggccttgct gattattctg
      961 gtcaataaac acaggggagc aatccccttt ttttacataa atcagacaga attacacacg
     1021 aggtaggggg atggttagaa gtttaaaagc aagactagaa aagttagaag agtatgctgg
     1081 aaagacggaa tatgcaacaa ctgatgaatg tatatcttta tatgtggatt gtttaagatt
     1141 tgcttttcag caagatgaaa taaagataga tatggatgat tttattaaaa caccatttaa
     1201 agatattaag ctagcagact ataagaaatc aaaaaaacgt atggatgaac ttagggaaat
     1261 gactgggata agtgagtttt cagaattaga gcaataaagc gaggtgaaca gtatttgaca
     1321 gaattaagca gaaaaaaaga aatatttatg gccaattatc tcatgactgg gaatgtaaaa
     1381 aaagctagtg aattaagtga gatcaccaga aagacggctt acaattactt gaacgatgca
     1441 acctttaaga gaatttaccg tgaaagacgt agcgaacaac ttaaggagtc tactacgctg
     1501 ttacagaatg caagcgttga ggcagtcaat gtattaaggg aaatcatgct tgataagaca
     1561 gtgagcccgt atgcgagaca acaaagcgcc caaagcattc taaacatggc ttataaggcg
     1621 gttgaaatgg ttgaggtagt ggagcaatta gaaatattag aggcgaagat actagatgaa
     1681 ggcgattaaa aaccgtttaa ataagttgaa taagttagcg gtggttgatg aagttgatac
     1741 cgtgtttttg acgcagattg atactggaca ttttgaggta agatatagga taggtggaaa
     1801 aaatcccaaa gatattatag aagtctatcc gacaaaagaa gaagcagaaa aagctatttc
     1861 agaaattaaa aatacaaaag gattgcggat attctgggac aatttggctt tattggagga
     1921 taattagagc ttatcattta tcaagcagat aagagaaata ataaccctcc ttaactcctt
     1981 gagaaacgac aagcagataa gcgaagtttt tagccctctt atttcggttc tactccttgc
     2041 agaatgacaa gcagataagg acgacaataa gccttcttaa ttgcttacaa aacgataggg
     2101 ggttaagacg aatttaaaag ctccttattt tcgtttttct ccttgtcaaa cgataaggaa
     2161 ataagaacta aaataactcc ccttattttc ctaccaaatg ataaggagat aagagcaatt
     2221 taaaagtctg ttatttttgg atttgttgct taccaaatga taagcagata agcaaagaaa
     2281 taacttgcct taattccttg cagaatgaca aggaaataag agagataaaa acatccctta
     2341 attgcttatc aaaccacaag gagatacggg ggctatctcc ctccccacgc acttctatga
     2401 gctttacacc gtcattgtac attttttctc acggggaagc atttgaagct agagcgaaca
     2461 tagagaaaac gaaaaaagcc aagacactct ctgtctcagc taatagttct cacaaagact
     2521 attatatcat acaggaggcc gagacatgac accagagcag gtaaaagaaa cactagaggg
     2581 cgttaagtgg ataaacaaag agatagaagg cttatattta gagcttgcaa ctttagaaag
     2641 tggtattatc aaaaagcaag aactaagcaa taccagagta caaacaagca gggtaaatac
     2701 ggcagagaat aatcttataa gtgttctgaa gctaaaagag gatacgcttc agaggattga
     2761 gcgacttact gaggagagaa tggaaatatc taggctgatc gataagctgg ccaatccgct
     2821 tgagcgttct gttctcaggc tgttctactt gaatgttctc gaagcttggc aggttgctga
     2881 agaaatagat aggtcaaaga ctacagtata ccttataagg caaaaagcca tagaacactt
     2941 agctggtctg gtacatgcag attgatttaa ccttaccaca gagactaatg tgtatagggt
     3001 gccctaggct tcatattcgc ctccctattt tgcttggttt tgtaccggac gattaaaaca
     3061 ccatggataa actagaagcc cttagaaacg aatcaggggg acttttgtga ggtcataatc
     3121 ttataaacca cgcgcttcat ggtataatat acctatcagc aaccagcaat aaaaaaagca
     3181 cgtttgaccg tgctagtttc ttgcctgctg aactcgtcat ttattgccct tttgtggggc
     3241 tttttttgtg gactttttga gaatttttaa gaacttttaa tgatttctaa tcaaatgaga
     3301 tttttcaaaa agtcagtaat atcaagggtt taggcgctat aaacaagttt aaaagcgtgt
     3361 agattacaat ctaacacaaa aagggataaa gtgctaaccc tctaactaag ggttctcagt
     3421 caataatttt attcattttt cgctcaaaat catatcgccc catcatgaca acatgctcac
     3481 aattactgca ttttattttg atatctgctc ctacacgtgt aatttcccaa cgattagcct
     3541 ttttaccagt cgacttgatt gtacaagcgt gtgatttttt catctcaaca aaatttccaa
     3601 cttgatacat actactctcc ttttcttttt cgattatatc ataaaagagg cgagccaggc
     3661 tcaacctctt tcacttaatt tgtacgaact ggtgtaatga gctgcatgaa gtcttcgtca
     3721 gtatctgctg gcacaagagt aaatggacga acagctgaga taaagctaat ggtcaccttt
     3781 tcgctattta aagctttaag agaatcaatc aagtaagttg ggttgaaact aatggtcaaa
     3841 tcttcaccag taacctgatc agtatcgatt tcttcgttta ctttaccaac ttctggagag
     3901 tgaacatggg cgctaacaac cccatcctta atttcaagtt tcacagtacc attttgagtc
     3961 gcacttgata aaagacgggc acgctccatt gactggcgta agtttaccac attaaaagta
     4021 atagtagtgt taaagtctgt tggaatcaag cgatctgtat caggatagtt tccttctagg
     4081 agacgagtat agaagctaat attttcgctt ctaaagagga tttggttatt ggcaaagaaa
     4141 atctctacag tttcgatatc atctgtaaat accgctgaaa attcgcgtag agaacggcta
     4201 ggaattacga catcaaaatc atcactattt ttttcaagag tcaatttttt ctggcttagg
     4261 cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt gactcaatac gaagtggaca
     4321 cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa aggctgtttc attaataatt
     4381 ttcttgagta attttgtttc aagtattaaa ggagtgcttg ctgaaatttc ttggattcgt
     4441 ggatattgtt cgctatcttt tccttttagg gtaatttctg atttgccact ggttaaaaca
     4501 atttgatttt gttcaatttc tttaaaatca agagtcacat caggta
//
LOCUS       DAVHIU010000127         4521 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVHIU010000127 DAVHIU010000000
VERSION     DAVHIU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.129"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            90..254
                     /locus_tag="VVN06_001835"
     CDS             90..254
                     /locus_tag="VVN06_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5563325.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            338..904
                     /gene="amaP"
                     /locus_tag="VVN06_001836"
     CDS             338..904
                     /gene="amaP"
                     /locus_tag="VVN06_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU5563326.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            916..1086
                     /locus_tag="VVN06_001837"
     CDS             916..1086
                     /locus_tag="VVN06_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU5563327.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1125..1733
                     /locus_tag="VVN06_001838"
     CDS             1125..1733
                     /locus_tag="VVN06_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU5563328.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1764..1967
                     /locus_tag="VVN06_001839"
     CDS             1764..1967
                     /locus_tag="VVN06_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5563329.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2077..2349)
                     /locus_tag="VVN06_001840"
                     /pseudo
     CDS             complement(2077..2349)
                     /locus_tag="VVN06_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2494..2940)
                     /locus_tag="VVN06_001841"
     CDS             complement(2494..2940)
                     /locus_tag="VVN06_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563330.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            3010..3669
                     /locus_tag="VVN06_001842"
     CDS             3010..3669
                     /locus_tag="VVN06_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5563331.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(3721..>4521)
                     /locus_tag="VVN06_001843"
     CDS             complement(3721..>4521)
                     /locus_tag="VVN06_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU5563332.1"
                     /translation="DLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERSG
                     IYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMNE
                     DGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEYR
                     AFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSLA
                     TIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT         1394 a          822 c          807 g         1498 t
ORIGIN      
        1 aaaaaaggag ggtaggcagt gttgtgatca attattgctg gaggtcttat tggtctcttg
       61 gcaggtaaaa tcactaaaaa agtagttcta tgggaatcat cgcaaatgta ttcgctggtt
      121 tagtcggggc atatgcagga caatctcttt taggtagttg gggtccatca atcgctggaa
      181 tggctttgct cccatctatt gtaggtgcag cgattgtgat tactgtagtg tcattcttta
      241 caggtagaaa gtaaactttt cgccagtaaa gttagcaaac tatttttaaa tcaatgacgg
      301 gaaaaatagt ttaaatgtta aatcgaaagg attgtatatg tcaaaagcaa agaaaatatg
      361 tttcattatt ttctgtattt taatcttgac aattttcctt cctgttttga tagattatca
      421 tcaagttagt gatctaggta ttcatctact tagctggaga cagaactccg tagttgaatt
      481 ctatcttgct agatatgtct tttgggggac agtggttcta tcaactttag ttttattatc
      541 cattttagtt gtgatgtttt atcctaaacg ttacttggaa atccaacttg aaactaaaaa
      601 cgatacatta aaattaaaga attcggcaat cgaaggtttt gttagaagtt tggtgagtga
      661 tcatagattg atcaagaacc caactgttca tgtaaattta cgaaaaaata aatgtttcgt
      721 tcatgtagaa ggtaaaattc ttccttcaga caacatcgct gacagatgcc aaataattca
      781 aaatgaaata actaatggat tgaagcagtt ttttggtatt gagcgtcaag taaaacttga
      841 agttgcagta aaaaattacc aaccaaaacc tcaaaacaaa aagactgtta gtcgtgtgaa
      901 gtaaggaagt aaaaaatgga atggcttaaa caatatcgat atccaattat cgctggtctc
      961 ataggcgtat ttctggcttg tttgattgtc tcctttggct tcttcaaaac aatatttgta
     1021 ttgattttag gagcactggg agttgcagct ggattatata tcgaaaaaaa ctatatagat
     1081 aaataaaaaa ataaaaatta ctaatttaat taaaggagtt tcatatgtca aacgaaaaaa
     1141 acacaaacac taacgtagaa aagaaagatg ctactgttgt agctcacgaa atcaaagggg
     1201 aacttactta cgaagataaa gttatccaaa aaatcattgg tctttcacta gaaaacgttt
     1261 caggtctttt gggaatcgat ggtggtttct tctcaaatct taaagaaaaa atcgttaaca
     1321 gcgatgacgt aacaagtggt gttaacgtag aagttggtaa aacacaagtt gcagttgact
     1381 taaacgttat tgttgagtac caaaaaaatg ttccagcttt atattcagaa atcagagaaa
     1441 tcgtatcttc agaagttgct aaaatgactg acttggaaat tgttgaaatc aacgtaaacg
     1501 ttgtcgacat caaaactaaa gaacagcatg aagcagactc agtaagcctt caagatcgcg
     1561 tatctgacgt tgctgaatca acaggagaat tcacttcaga acaattcgaa aaagctaaat
     1621 ctggtcttgg atctggtttc tcaactgttc aagaaaaagt tagcgaaggt gtagaagctg
     1681 ttaaaggtgc agcaaatggt gtagtatctc acgaaaacac tcgtgtaaac taagataaaa
     1741 taaatataac aggagaaatt atcatgtcag tagaagaaaa attaaatcaa gctaaaggtt
     1801 ctattaaaga aggtgttggg aaagccatcg gtgatgaaaa aatggaaaaa gaaggagcag
     1861 ctgaaaaagt tgtttctaaa gtaaaagaag ttgccgaaga cgctaaagac gctgtagaag
     1921 gtgctgtaga aggtgttaaa aacatgttga gtggcgacga taaataaggt taaaagttac
     1981 tttatctttt tggtaacatt agtcaaaaga gtctgagtca agatgattct cagaaaacaa
     2041 aaagctagag attcccaatt gcggaactct agctttttaa ttttgcctct ttctcttatt
     2101 atatttcagc aggttgttgg ccatgagtat gaatcccatg tcaattctca cttgacgctt
     2161 acctctcaga tgacatctct tataacccaa acaaaccttt atctgcccaa agacagattt
     2221 catatcaatc ttacgtttag cgaaaatttg tctacccttg gaagataaaa gtgcctgata
     2281 ttctttagtt tttaaacact ggtaacgttc attcatatac agtccctttt gaggggctga
     2341 ttcaggttca taatcgcagt caacattgat ttcaaggctg tttgctttct atctcccctt
     2401 tttcataatg tatagtaaaa tgaaataata acaggacgaa tcgatcggga cagtcaaatc
     2461 gatttctaac aatgttttag aagcagaggt gtactattct agtttcaatc tactatattt
     2521 tcgtacaggt gcttcaacca tttgaacaat ttcaaatcct tctttttggt aaagattctg
     2581 agctctttga tttgcctcga agacatttag agaaatacta tctatatctt cattttcaaa
     2641 ggccaaacta acaaatttcc ttaaagcctg gctacctaag tcttgctcct gtttctgggg
     2701 gttgataaaa aatctcccga tatgaagatt gctgtcttct agcctgattt tctggataag
     2761 tcccacaaac tcttgttcat caaagattga aaagactcct tccaaggctt gaagggtcag
     2821 tggaaaagga atccttggtc ccatccattg ttcttgaaag actttgccta aggagttgga
     2881 ccattggcat acgagctgag cgttttctat actcaccttt tcttcaaaac gaattgtcat
     2941 cttttcctca ccatcttatc tatgtttctc cattatacta tttctcccat tttttacgaa
     3001 tagataagta tgattgattt ttattttttt ctcgtcggga gcattctcgc ttcctttctt
     3061 ggtttggtca ttgaccgttt tccagagcaa tccattatca gttcagccag tcactgcgat
     3121 tcctgtcaga ctcgcttgcg tcccttagat ttgattccga ttctctcaca ggtcttcaat
     3181 cgctttcgct gtcgctactg caaagttcgc tatcctgtct ggtatgccct ctttgaatta
     3241 gtcttaggac tcctctttct gctttactct tgggaattgc tttccttggg gcaagtcgtc
     3301 ctaatcaccg ctggtttgac cttgggtatc tacgactttc accatcagga atatccctta
     3361 ctggtctgga tgactttcca cctaatccta atagcttcct ctggctggaa tctggtcatg
     3421 gtctccttcc ttgctcttgg aattttggct cattttatcg atatccgcat gggcgcagga
     3481 gatttcctct ttttagcttc ttgtgctctc gtctttagcg taacggagtt actgatcttg
     3541 attcagtttg cttctgcgac gggtatcctg gcctttatcc tgcaaaagaa aaaggaaaga
     3601 cttcctttcg tgcctttcct cttacttgct acttgtttga ttatttttgg taagctactg
     3661 cttgtttgat aaagtccaat ttctgccata tgtccttcat gaaattattt cacagttaaa
     3721 ttataaatta tttcttttgt acaaagggat gatgttatca aatcgatctg ttcttctatc
     3781 ttcttgatac tgatcaaaaa atttcatttc gactgaaaat atttcgctta taaactgtaa
     3841 acgaatactt tgtttagaca ttatagtcgc tagactgact agatgattac tcaaaacgac
     3901 gtccagaata ctctttactt tgcttggttt tttaacaaaa atttgatcat ccaaaggttc
     3961 aatcattttg taaccttttt gcgcaatttg acgataaaag taagaatgtt gctttggagt
     4021 caataatcct aacttaaaag ctcgatactc taaagcctgt atcgaaacat tcaaatccga
     4081 cttcaataaa atataactat caggattgct gacacgcttg ccaaccctct cttcaaattt
     4141 gactaaaaac tcttcttttg gcaataaaaa acatgatgca aaataatttg cttcttgctc
     4201 caaacgatcg ccatcttcat tcatatcttt atatttatgt aaaagaatat gtcctagctc
     4261 atgagctaag tcaaaatttc gacgtacaga tgatttattc gttcccaaca caatataagg
     4321 tcttcccaat tttgaccatg cgctataagc atcagcttgg ccattaatta atcgttccac
     4381 gatatagatg cctgaacgtt ctaatttata aagcaaatca tgattatctt ttgaaatacc
     4441 taatttttcc ctggcataaa gagccaattc ctcaatggat tctcccttat gataagattc
     4501 actcactaca ttacttaggt c
//
LOCUS       DAVHIU010000128         4428 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVHIU010000128 DAVHIU010000000
VERSION     DAVHIU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.130"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>4428
                     /locus_tag="VVN06_001844"
     CDS             <1..>4428
                     /locus_tag="VVN06_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5563333.1"
                     /translation="KSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALNISDKEKVVL
                     DKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKAVVSKNPVTDNNTSNEEARIKEDS
                     NQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTYDR
                     IFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAP
                     FGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPH
                     AFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIF
                     SYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRK
                     AGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVE
                     FDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRG
                     KIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKS
                     QVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDE
                     KEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGK
                     STYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNT
                     ARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSIS
                     LKEIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQI
                     VPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEE
                     MEALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPK
                     IPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSGSK
                     IANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSRE
                     HFIRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIR
                     GQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKD
                     TYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGE
                     GQGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMI
                     SYYLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKS
                     LFNIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDD
                     GTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVS"
BASE COUNT         1730 a          662 c          871 g         1165 t
ORIGIN      
        1 aaaaagtaca gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa
       61 tgaggtagtc ttagcagaca catctaactc tgaagatgct ttaaacatct ctgataaaga
      121 aaaagtagta ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc
      181 tatagaaact tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagcagt
      241 tgttagtaaa aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga
      301 agattccaat caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa
      361 tcccaaaaaa gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga
      421 aaaagcaatc aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag
      481 aatttttaac ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat
      541 agaaggtatt acatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag
      601 aaaggaaatt ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg
      661 gaaaaatttt gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag
      721 acataaggct atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga
      781 cttaaaagga actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta
      841 ttataatggt ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc
      901 acatgggatg catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa
      961 ctttaacggc atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc
     1021 tgacgcagga tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat
     1081 caaacacaac gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg
     1141 tgagaaatat tggcaagcta ttagggcatt aagaaaagca ggcattccaa tggttgtcgc
     1201 tacgggtaac tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca
     1261 tctgaaaatg accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc
     1321 ggtcgcttct gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag
     1381 ttttaaatac agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga
     1441 tggaacaaaa gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga
     1501 tttgataggt ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga
     1561 tttaaaaaat gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa
     1621 tactgtaaat tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc
     1681 ggatgaaggt actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg
     1741 gaacatgatt aatcctgata aaaaaactga agtcaaaaga aataataaag aagattttaa
     1801 agataaattg gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa
     1861 tgtaggtgac gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta
     1921 taaagaagat atcatcgttc cagcaggatc cacatcttgg gggccaagaa tagatttact
     1981 tttaaaaccc gatgtttcag cacctggtaa aaatattaaa tctacgctta atgttattaa
     2041 tggcaaatca acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc
     2101 ttctactgtt ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa
     2161 tcttaaggga gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac
     2221 tgctcgacct atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc
     2281 tagacaacag ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc
     2341 aactttcaaa aacactgatt ctaaaggttt ggtaaattca tatggttcca tttctcttaa
     2401 agaaataaaa ggtgataaaa aatactttac aattaagctt cacaatacat ccaacaggcc
     2461 tttaaccttt aaagtttcag catcagcggt aactacagat tctctaactg acagactaaa
     2521 actggatgaa acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat
     2581 tcatccagaa aaagtcaaag gggcaaatat tacatttgag catgacactt tcactatagg
     2641 ggcaaattct agctttgatt taaatgcggt tataaatgtt ggggaggcta aaaataaaaa
     2701 taaatttgta gaatcattta ttcattttga gtcagtagaa gaaatggaag cattaagctc
     2761 caatggcaag aaaaccgact tccaaccatc tttatcaatg cccctaatgg gatttgctgg
     2821 gaattggaac cacgaaccaa tccttgataa atgggcttgg gaagaaggat caaaatcaaa
     2881 aacaatggaa ggttatgatg atgacggtaa accaaaaatt ccaggaacct taaataaggg
     2941 gattggtgga gaacatggta tagataaatt taatccagca ggagttatcc aaaatagaaa
     3001 agataaaaat agaacatctc tagatcaaga tccagactta tttgccttta acaaccaagg
     3061 tgttcatgca gaatccacta gtggttctaa gattgctaac atttatcctt tagattcaaa
     3121 tggaaatcct caagatgctc aactagaaag aggactaact ccatctccac ttgttctaag
     3181 aagtgcagaa gaaggactaa tttcaattgt aaacaccaac aaagaaggag aaaaccaaaa
     3241 agaccttaaa gttgtatcaa gagagcactt cataagagga attctaaatt ctaagggaaa
     3301 tgacgctaag ggtatcaaat catccaaact aaaagtttgg ggtgacttga aatgggatgg
     3361 actcatctat aacccaagag gtagagaaga aaatgcccca gaaagtaagg ataaccaaga
     3421 tccagctact aagatacgag gacaatttga accgattgcg gaaggtcaat atttctataa
     3481 atttaaatat agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat
     3541 tgataacaca gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagct
     3601 aatcacaaag gatacttatc acaaggtaaa agatcaatac aagaatgaaa ctttatttgc
     3661 tagagatcaa aaagaacatc ctgaaaaatt tgatgagatt gcaaatgagg tgtggtatgc
     3721 aggagctgct cttgttaatg aagatggaga ggttgagaaa aatcttgaag taacttatgc
     3781 aggtgagggt caaggaagaa ataggaagct tgataaagat gggaatacta tctatgaaat
     3841 tagcggagca ggagatctaa gaggaaaaat catcgaagta atcgccctag atggagcaag
     3901 caactttacc aaaatccata ggattaaatt tgctaatcaa gctgatgaaa aagggatgat
     3961 ttcctattat ctagtagatc ctgatcagga ttcatctaaa taccaaaagc ttggcgaaat
     4021 tcctgaatct aaatttaaga atttaaaaaa tgttaaagat gatagcctaa acaaagagac
     4081 agctgaggtg gaaaataacc ttttagtaga taatcagtct atcgaaggaa aatctctctt
     4141 taatattcat aaaactattt caacaattag agactttgaa aataaagacc taaagaaact
     4201 cattaaaaag aaatataagc aagaagacga ctttgtaaat ggtggaacaa gaacagtaga
     4261 gcgtgattat aaatacgatg ataaaggaaa tataatagcc tacgatgatg ggactgatct
     4321 agaatatgaa actgagaaac ttgacgaaat caaatcaaaa atttatggtg ttctaagtcc
     4381 gtctaaagat ggacactttg aaattcttgg aaagataagt aatgtttc
//
LOCUS       DAVHIU010000129         4260 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVHIU010000129 DAVHIU010000000
VERSION     DAVHIU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.131"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            262..2595
                     /locus_tag="VVN06_001845"
     CDS             262..2595
                     /locus_tag="VVN06_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5563334.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2608..4071
                     /locus_tag="VVN06_001846"
     CDS             2608..4071
                     /locus_tag="VVN06_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU5563335.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILDPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4071..>4260
                     /locus_tag="VVN06_001847"
     CDS             4071..>4260
                     /locus_tag="VVN06_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU5563336.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         1491 a          641 c          885 g         1243 t
ORIGIN      
        1 taaatgtgat tatttatttt tgaaaaatag ctcaccagcc cgactgaaag tgcttataga
       61 atgataataa gtcgcctgcc gaaaacagcg gaaaatagcg gtgttatgcg gagataatct
      121 gacgcgatgc gaaagtatat tgcatactta ttttcaacaa tttagcagag tatttttata
      181 agtgtgatat aatagaagta taatttgttc tgatagttta ttttatggag aagtagattt
      241 ttagaatgcg gagggttcaa tatggttgag tttataaagt ctaagaaaga aatgagtgag
      301 gaggatatta aagcaaattt catcactcct gctattgtat ccaaaggatg gaaaaatggt
      361 gagcatatcg cttacgaaga atacttcact gatggtcgaa ttgaggttag aggagataag
      421 gctcgtcgta aagaaggaaa aaaatcagac tattcactgt attaccaatt tggaactcga
      481 attgcaattg ttgaggcaaa ggataataaa cacagcgttc gagcaggatt acaacaagct
      541 attgaatatg gagagatttt agatgttcca tttgtttatt cttcaaatgg tgatggcttt
      601 attgaacacg accgtatcac gagagaagaa cgtgagctgg agttagacga attccctact
      661 cgtgaagaat tattttctcg tatgacgaag gaaaaaggat tgacgtacga aattacagaa
      721 gctatctcaa ctccatacta tacagacgcc ttctcaatga aaacgccacg ctattatcag
      781 caaatagcta tcaaccgtac tattgaaaca gttgccagag gacaaaaacg agtaatgttt
      841 gtgatggcaa caggaacggg gaaaacgttc atggcttttc aaattattca tcgccttcga
      901 aaagctggtt tggctaaacg agttttattc ttagcggata gaaacatctt agtagaccaa
      961 acgatggctg aagactttaa gccattcgaa aaggtaatga cgaaaattac accaaaactt
     1021 ttgactgctc ctgaaaaatt aaattctttt gaaatttatc tagggcttta tcagcaacta
     1081 actggtgaag atggaactga aacacattat caaaaatttg acaaagactt ctttgattta
     1141 atcgtaattg atgaagcgca ccgtggttca gctaaggaaa acagtaactg gcgtaagata
     1201 attgattatt tcagttctgc gacacagatt gggatgaccg ctactcctaa agaaaccaag
     1261 aatgcttcca atacggaata ctttggtgag ccaatctata cttatagttt aaaacaggga
     1321 atcgaggatg gttttttggc tccatatcgt gttatgaggg ttaatttaga tgtggatgtg
     1381 gatggttatc gtccagaaac tggaaaagtt gatactaacg gacaattaat agaagatagg
     1441 tactacggca ggaaagattt tgataaaacc attgtcattg atgatagaac gcaaagagtt
     1501 gccaagtttg tttctgatta tatgaagcaa agcaatgcac gatttgataa aacaattgtt
     1561 ttttgtgttg atattgacca tgccgagcga atgcgtgctg cacttgtaaa agagaatcta
     1621 gacttagtcc aagaagacta tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga
     1681 aaagctcaac tggataactt tatggatgtc aattctaatt ttcccgctat tgtaacaacg
     1741 tctaaattat taacgacagg agttaatgct aaaacatgtc gtttgattgt tttagactct
     1801 aatatccaat ccatgactga atttaaacaa attattggtc gtggcacacg tctttatcct
     1861 caaaagggga aagaattttt tacgattatt gattttcgaa atgttaccaa tttgtttgct
     1921 gaccctgatt ttgatggtga tcctgtgaag gtgctggaaa caggtgcgaa aacagtcagt
     1981 ggttctacgc ccggtttcgt agatgaggaa ggtgacccag tagaaaaata tatcgttaca
     2041 gacaagcagg ttaccattct taattctact gttcaagtat tagatgaaaa cgggaaactg
     2101 attaccgaaa gcctgaccga ctacactcgg aagaatatct taggtagcta cgccactttg
     2161 aacgatttta tcacagtttg gcatacggcg gataaaaaga agcttatctt agacgaactt
     2221 tataaaaaag gagtttatct agatgctatt cgagagtcgg agggaatatc agaacaagaa
     2281 atcgatgatt ttgatttact cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg
     2341 gaacgtatca ataaactcaa acaaagcgga tatttatata aatatagtga ggaagcgcgt
     2401 gctgttttgg aaattttact gaacaaatac atggataaag gtattggaga actcgaaagc
     2461 attgaaacat taaaacttcc agaatttcag atatatggtg gaaccttcaa aatcatcaat
     2521 acttattttg gagataaaaa acgatattta caagcaatta aagaattgga gcaagagcta
     2581 tttacagtag cttaatgaaa ggaaagtatg tcaattacat catttgtaaa aagaattcaa
     2641 gatatcactc gaaacgatgc tggtgttaat ggtgatgctc aacgtattga gcaaatgtct
     2701 tggttattat tcttaaaaat ttatgatagc cgtgaaatgg tttgggaatt agaagaagac
     2761 gagtatgagt caattatccc agaggaatta aaatggcgaa attgggctca tgctcaaaat
     2821 ggggaacggg tattgacagg cgatgaatta cttgattttg tcaataacaa gttattcaaa
     2881 gagttgaaag agcttgaaat aacttcaaat atgcctattc gaaaaacgat tgttaaatca
     2941 gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct tgttacgcca agtcatcaat
     3001 gttattgatg aagttgattt caatagccct gaagatcgtc attcgtttaa tgatatttac
     3061 gaaaaaattc ttaaagatat tcaaaatgct gggaactcag gagaatttta tacgccacgt
     3121 gcagcgactg attttattgc cgaagttctt aacccaaaac ttggagaatc aatggcagac
     3181 cttgcttgcg gaacaggagg cttcttgact tcgactctga accgtttaag tagtcaacgt
     3241 aaaactagtg aagataccaa aaaatataat acagctgttt ttggtattga aaagaaagca
     3301 tttcctcatc ttttagcagt tacaaatctg tttcttcacg aaattgatga ccctaaaatt
     3361 gttcatggaa atactttgga gaaaaatgtt cgtgaatata cggatgatga aaaatttgac
     3421 attattatga tgaatccacc ttttggaggg tcagaattag aaacaataaa aaataacttt
     3481 ccagcagaat tacggagttc tgaaacagct gatttattta tggctgtcat tatgtatcgt
     3541 ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg gttttctatt tggtgaaggt
     3601 gtaaaaactc gcttgaaaca aaaactggta gatgagttca acttgcatac gattattagg
     3661 ttgcctcata gtgtctttgc accgtataca ggaatccata cgaacattct tttctttgat
     3721 aaaacaaaga aaacagaaga aacttggttt tatcgtttag atatgccaga tggttataaa
     3781 aatttctcga aaactaagcc gatgaagtca gaacacttca atcctgttcg tgactggtgg
     3841 gaaaatcgtg aagagattct ggaaggtaag ttctacaaat ctaaatcatt tacacctagt
     3901 gaattggctg agttgaatta taatttagac cagtgtggct ttccaaaaga ggaagaggaa
     3961 atcttagatc cctttgagtt gattcagaat tatcaagcgg aaagagcaac tttaaatcat
     4021 aagattgata atgtattagc tgatattttg cagttgttgg aggacaaata atgacaccag
     4081 aacaacttaa agcaagtatt ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc
     4141 ccaatgacga acctgcaagt gaattattaa agagaattaa agctgaaaaa gaaaaactta
     4201 tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga gatatttcgt ggtgatgatg
//
LOCUS       DAVHIU010000130         4066 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVHIU010000130 DAVHIU010000000
VERSION     DAVHIU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.132"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            121..1632
                     /locus_tag="VVN06_001848"
     CDS             121..1632
                     /locus_tag="VVN06_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU5563337.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1654..2985
                     /locus_tag="VVN06_001849"
     CDS             1654..2985
                     /locus_tag="VVN06_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5563338.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQLKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHKRSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            3080..>4066
                     /locus_tag="VVN06_001850"
     CDS             3080..>4066
                     /locus_tag="VVN06_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5563339.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKVLLEGCSQRSVSLDYALPNQGMLSFWLAQYKKN
                     GYTIVEKTRERVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIK
                     LARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRV
                     QGLIKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPA
                     STQKLYLSPVLDGFNSEIIAFNLSTSPNLEQVKSMLEQAFTEKYYENTILHSD"
BASE COUNT         1209 a          808 c          858 g         1191 t
ORIGIN      
        1 tattagtttt ttagacttta atatggtaaa acaaatataa atagaaagcg ttttcttaaa
       61 tgtttattta agaaagtagt tggtttttta cactttgtta gacatcagga ggaaaaacaa
      121 atgagtgaaa aagctaaaaa agggtttaag atgccttcat cttacaccgt attattgata
      181 atcattgcta ttatggcagt gctaacttgg tttatccctg cgggggcctt tatagaaggt
      241 atttacgaga ctcagcctca aaatccacaa gggatttggg atgtcctcat ggcaccgatt
      301 cgggctatgc taggtactca tccagaggaa ggttcgctca ttaaagaaac gagcgcagcg
      361 attgatgtag ccttcttcat ccttatggtt ggggggttcc ttggcattgt caacaaaact
      421 ggtgctcttg acgtagggat tgcctctatc gtgaagaagt ataagggccg cgaaaaaatg
      481 ttaattttgg tactgatgcc tttgtttgcc ctcggtggta caacttatgg tatgggtgaa
      541 gaaacaatgg ccttctatcc actccttgtg ccagttatga tggccgttgg ttttgatagc
      601 ctgactggtg ttgcaattat tttgctcggt tctcaaatcg gctgtttggc atctactctg
      661 aatccatttg cgacaggtat tgcttcagcg actgcgggag ttggtacagg ggacggtatc
      721 gtacttcgtc tgatcttctg ggttaccttg actgctctta gtacttggtt tgtttaccgt
      781 tatgcggata agattcaaaa agatccgact aagtcactgg tttatagtac tcgcaaagaa
      841 gatttgaaac actttaacgt agaagaatct tcatctgtag aatctacact tagcagcaaa
      901 caaaaatcag ttctcttctt atttgtgttg acattcatct tgatggtatt gagcttcatt
      961 ccatggacag accttggcgt taccattttt gatgacttta atgcttggtt gactggtctt
     1021 ccagttattg gtaatattgt cggttcatct acttctgcac taggtacttg gtacttccca
     1081 gaaggcgcaa tgctctttgc ctttatgggt atcctgattg gtgttattta tggtcttaaa
     1141 gaagataaga ttatctcttc cttcatgaat ggtgctgctg acttgctcag tgttgccttg
     1201 atcgtagcga ttgctcgtgg tattcaagtt atcatgaacg acggtatgat taccgataca
     1261 atcctcaact ggggtaaaga aggcttgagc ggtctatctt cacaagtctt tatcgttgta
     1321 acttatatct tctatctacc tatgtcattc ttgatcccat cttcatctgg tcttgccagc
     1381 gcaactatgg gtatcatggc tccacttgga gaatttgtaa atgtccgtcc tagcttgatt
     1441 atcactgctt accaatctgc ttcaggtgtc ttgaacttga ttgcaccaac atctggtatt
     1501 gtgatgggag ctcttgcact tggacgtatc aacattggta cttggtggaa attcatgggc
     1561 aaactcgtag tcgctattat tgtagtgacc atcgcccttc ttctccttgg aaccttcctt
     1621 ccattcctat aaaatagtga gtgaggtgat tccatgaaaa tagatataac aaatcaagtt
     1681 aaagatgaat ttcttatatc attaaaaacc ttgatttcct atccttcagt actcaatgaa
     1741 ggagaaaatg gaacaccttt tggacaagca atccaagatg tcctagaaaa aactttagag
     1801 atttgtcgag acataggttt cactacctat cttgacccta aaggttatta cggatatgca
     1861 gaaatcggtc agggagcaga gcttctggcc attctctgtc atttggatgt tgttccatca
     1921 ggtgatgaag cagattggca gacaccgcca tttgaagcaa ctatcaaaga cggctgggta
     1981 ttcggacgtg gtgtccaaga tgataaaggc ccttcgctcg cagctctcta tgcagtaaaa
     2041 agcttgctgg accaaggtat tcagttgaaa aagcgcgtac gctttatctt tggtaccgat
     2101 gaggaaaccc tctggcgctg catggcacgc tacaatacca tcgaagaaca ggccagtatg
     2161 ggctttgcac ctgactcatc ttttcctctg acctatgctg aaaaagggct tctacaggtc
     2221 aaacttcatg gccctggatc ggatcaacta gagcttgaag taggaggcgc ctttaacgtt
     2281 gtaccagaca aggccaacta ccaaggtccc ctctatgaac aggtttgtaa cgatctcaaa
     2341 gaagctggtt atgattacca atccactgaa caaaccgtaa cggttctcgg agtgccaaag
     2401 catgctaagg atgctagtca aggtatcaat gctgtcatcc gactagctac cattcttgct
     2461 cctctccaag aacaccctgc tctcagcttt cttgcaacac aagcaggtca agacggcaca
     2521 ggaagacaaa tctttggtga tatagcagat gaaccttctg gtcacctatc ctttaatgtc
     2581 gcaggtctca tgatcaatca taaacgttct gaaatccgta ttgacattcg gactcctgtc
     2641 ttagctgaca aggaagaact agtagagttg cttacaagat gtgcacaaaa ctaccaactc
     2701 cgctacgaag agtttgacta tctagcgcct ctatacgtcg caaaagacag taaactcgtt
     2761 agcacactga tgcaaatcta ccaagaaaag actggcgata acagtcctgc tatttcatcc
     2821 ggtggtgcca cttttgctcg caccatgcca aattgtgtag ccttcggcgc cttattccca
     2881 ggagcgaagc agacagaaca tcaggcaaat gaatgtgccg ttctagaaga tttgtaccgt
     2941 gctatggata tttatgccga agccgtctat cgacttgcaa cttaatcagg caactgtttc
     3001 tacaaaaaaa aatcgaccga ttaatgaact gcaccccaaa agttagacag aataaatcta
     3061 acttttgggg tgttttatta tgaaattgag ttatgaagat aaagttcaga tctatgaact
     3121 aagaaagcaa ggacaaagct tcaaacagct ttcaaaaaga tttggtgtgg atgtttctgg
     3181 tctaaagtac atggtgaaat taattgaccg ttatggcata gaaatcgtta aaaaggggaa
     3241 gaatcgtcac tattcttcta aattgaaaca agaaatgatg gataaagtct tactggaagg
     3301 ttgttcacaa agaagtgtta gtcttgatta tgccctccca aaccaaggaa tgctttcatt
     3361 ttggctggca caatacaaga aaaacgggta tactattgtt gagaaaacaa gagagagagt
     3421 acctgagagc ggagaatgcc atcctaaaaa agttaagaga actccgattg aaggaggaaa
     3481 aagagaaaga agaaagacag aaattgttca agaattaatg actgagtttt cgttagttct
     3541 tcttctaaaa tccattaaac tagctcgttg gacctactac tatcacttga aacagctaga
     3601 caaaacagat aaggaccaag agcttaaagc tgaaattcaa tccattttta tcgaacacaa
     3661 aggaaattat gcttatcgcc gggttcattt agaactaaga aatcgtgctt atctggtaaa
     3721 tcataaaaga gttcaaggct tgataaaagt actcaattta caagctaaaa tgcgacagaa
     3781 acgaaaatat tcttctcata aaggagacgt tggcaagaag gcagagaatc tcattcaagg
     3841 ccaatttgaa ggctctaaaa caatggaaaa gtgctacaca gatgtgacag aatttgccat
     3901 tccagcaagt actcaaaagc tttacttatc accagtttta gatggcttta acagcgaaat
     3961 tattgctttt aatctttcaa cttcgcccaa cttagaacaa gtgaagagta tgttagaaca
     4021 ggcctttaca gagaaatact atgagaatac gattctccat agtgac
//
LOCUS       DAVHIU010000131         4053 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVHIU010000131 DAVHIU010000000
VERSION     DAVHIU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.133"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..401
                     /locus_tag="VVN06_001851"
     CDS             <1..401
                     /locus_tag="VVN06_001851"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5563340.1"
                     /translation="EFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIPLIASKMA
                     KSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDTIEQAI
                     ERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(726..2675)
                     /gene="mutL"
                     /locus_tag="VVN06_001852"
     CDS             complement(726..2675)
                     /gene="mutL"
                     /locus_tag="VVN06_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5563341.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            3031..3471
                     /locus_tag="VVN06_001853"
     CDS             3031..3471
                     /locus_tag="VVN06_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563342.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            3468..3926
                     /locus_tag="VVN06_001854"
     CDS             3468..3926
                     /locus_tag="VVN06_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU5563343.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT         1236 a          908 c          807 g         1102 t
ORIGIN      
        1 atgaatttat aacttcaaaa ttattatctg gagcactaga taatctcaaa agagagaatg
       61 taaacgagaa agatatagag gtagcctggg ttccaggagc ttttgaaata ccactgattg
      121 catcaaaaat ggcaaaaagt aaaaaatatg atgcaattat ctgcttggga gctgtcatta
      181 gagggaatac aagtcattat gattatgtat gtagcgaggt atctaaggga atcgcccaaa
      241 tcagtttaaa tagcgaaatt cctgttatgt ttggtgtgct aacgacagat acaattgaac
      301 aagccataga acgagctggt accaaagcag gaaataaggg ttctgagtgt gcacaaggag
      361 ctattgaaat ggtcaaccta attcgtacat tagacgcata gaacagactc tacttttaga
      421 cgaataaaga aacttggcaa aatcgcaatt taatactctg tgaaaatcat agagcaaact
      481 aggaaaccag tcgcagagtg gtaaaacaca acttcgatgt tacagacaaa actggcaaaa
      541 ttagctcaaa acactgtttt gagattactg ataaaactga cgtggtttaa aaagagtttc
      601 gaaaagaatt aggaaaacat gaagtaacta tttccatgat aaaacgcata aaatcaaggt
      661 ttttgaacac ctgattttat gcgtttttct gattttgaaa atttttccca gactttttta
      721 tacttttaat atttccccaa ctcacggaga ctggtgtgat tttcctgaat acgtcggaac
      781 atcttttcca tatccgactt ggtaaaatgc accaaaacag gacgtccgtg aggacagtta
      841 tagggattgt cacattgaga aagctgatag aggagttgtc tagctgaatg atcatcaata
      901 cgatgattgg ccttgataga tcgcttgcaa gacatcatga tagccagctc tgctcggtat
      961 ttcttgatag aaacttcctt ggtcaaaagg agcatgtcgc acatctcata gatgcctgat
     1021 tcaatctctt cttctgccat ccaaatagga tgttcacgta gaataaattg attttctccg
     1081 tactctgcta gaaagacgcc cacttcctct aagagaggca ttctttcctt gagacgcagg
     1141 gcatcatccg caggaaattc aaagatatag ggcactagga gttgctgctg actctggtca
     1201 acattgccaa tgctttcacg gtactcctcg tacttgaccc gttcctgagc agcgtgctga
     1261 tctatgatgt aaagtccatc tcgcccttgg gcaaagagat aagtcccgtg catttgtccg
     1321 aaaaactcca actctgggaa gctggatgct tcttctcgct ccagtttgtc ataagcctta
     1381 tcgatgctag caagatctaa ctctggatgg tctagctggt cgtagttagc aggctttctc
     1441 tctgcaaaat gcagttttgc tggcttggtc aatcggtcca aggtttcctt ggcaaacagg
     1501 gtcaaatcct gcccttcatc agtcaattct acctgataat cagctacttc agtttgacta
     1561 ggtcttgacg gctcagtttt ctcatagtag agcgtatttt ctttgagtgg gagaatagtt
     1621 tgctccacct tctcacgatt gcgcacggtc gatttggcaa gattttccaa ggcatctgga
     1681 atcaaggttt gttccttgag actatttgca atagcttctg aaaccagggt catcagttct
     1741 ttttccttgg aaatccgcac ctcttgctta gttggatgca cattgacatc cgctagataa
     1801 gggtcgatat ggatgtgaat gacagccagt ggaaaacgtc caaccataag cttgcttccg
     1861 aaaccatcca aaatagcacg attgagcagg aagttcttga tataacggcc attgatgaag
     1921 aggctgatat aattgcggtt agcccgagtc aactcaggta aagacacaaa acctgaaatt
     1981 tcgaaatcta ggtcagagtt ctcaatttca atcatcttct tggcactgac caaaccgtaa
     2041 atccctgcga ttgcttggcg caattgacca gtccctgctg tccgcgtcat ttccttgcca
     2101 tcactaatca agctaaaaga aatctcagga tgggccaagc ccagacggtt gacaatatca
     2161 atgatatgag acaactccgc ttgctggctc ttcatatact tgagacgggc aggcgtgttg
     2221 aaaaagagat cctccacaca aaccttggtt cccacaggac tagtcgctgg gatgacttcc
     2281 tcaacttcac ccccacgcgc gactaacttg gttccatgac tagcaccatc caccgccgtt
     2341 aacagagtca agacactaac agacgcaata gaaggcaagg cttcaccacg aaaaccaagc
     2401 gtccgaatcc gaaagagatc tgcttgattt tttatcttac tggtcgcatg gcgacgcagg
     2461 gccaactcca cctcatcgtg ggcaattcca tgaccgttat ccgtgatttg aaccttcttg
     2521 agaccagctt cctcaatctc aatgataatc tgactagagc ccgcgtcaat ggcattttct
     2581 accaactctt taaccacact ggcaggacgt tcaatgacct ccccagctgc gatttggttt
     2641 gccagcatct ctggcaattc aataatatga gacatctttc agcccctttc tgtatctatt
     2701 ttcctagaaa ttaattagtg ctattatacc atatttcaga aagaacagaa aaactccacc
     2761 acataggagc ccaatccctc cacatagaca aagtcccttg ccagagggag taaaatagtg
     2821 gtatactcaa taaaaatcaa agagcaaact agaaagctag acgcagacag ctcaaaacac
     2881 tgttttgagg ttgtagataa aactgacaaa gtcaacaact atcctacggc aagacgacgc
     2941 tgacgtggtt tgaagagatt tttgaagagt attaataaag gtgtacaaga agtgagatta
     3001 attttgtatg aaaaacaagg agagaaattt atgaaagtag aactacagat tagtgagact
     3061 tacgaggagg aaaagctgat tgtccaagca cctcaagaaa ccgaaaaagt ccagaaagtc
     3121 atcgagttcg cagaaaatct ggaccaaaga gaaaaaatca aaggaaaaat cgatgatcag
     3181 gtctatctag ttgagattgg caagattcag cgcttctata tcgagaatcg gaaggttcta
     3241 gcagaaaccg cgagtcagac ctacagcatt gatttgcgac tctatcaggt tcttaaactc
     3301 ttgccaagca attttatcca aatttcccaa tcagaaatca tcaatatcga ttccatctct
     3361 cacctcaagc tcacgccaaa cggtctggta gaaattttct tgaaaaacga aagcttcacc
     3421 tactcttcac gccgttatct aaaaaccatc aaggagaaat tagaactatg aaaaaacaag
     3481 tatttcacga tgcagctgcc ggtgttctta tcggcctcat cctctccatc ctcttttcac
     3541 tcatttatgc accaaatacc tacgcaccac taaatcccta ctctctcatc ggccaagtga
     3601 tggatcagca tcaggttcac ggtgccctgg tcttgctcta ctgcacactt atctgggcag
     3661 ccatcggtat gctcttcaac tttggcaacc gcttattcag ccgtgactgg agcatgcttc
     3721 gtgccactct gactcatttc ttccttatgc tggctggctt tgtcccacta gcaactcttg
     3781 ctggttggtt ccccttccac tggattttct acctccagct cattatcgag tttgcgattg
     3841 tctatctcat catctgggct attctctata aaagagaggc taaaaaagta gatcacatca
     3901 atcaactctt ggagcataga aaataggaac ctcataataa gcaaaaccga gaaagaaact
     3961 tctcggtttt gctttatcct gctgcacctt actatagtga aatgaaataa gaacaggata
     4021 aatcaatcag gacagtcaaa tcgatttcta aca
//
LOCUS       DAVHIU010000132         3989 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVHIU010000132 DAVHIU010000000
VERSION     DAVHIU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.134"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            178..492
                     /locus_tag="VVN06_001855"
     CDS             178..492
                     /locus_tag="VVN06_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5563344.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            508..894
                     /locus_tag="VVN06_001856"
     CDS             508..894
                     /locus_tag="VVN06_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5563345.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            923..2308
                     /locus_tag="VVN06_001857"
     CDS             923..2308
                     /locus_tag="VVN06_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5563346.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVQAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2311..2463
                     /locus_tag="VVN06_001858"
     CDS             2311..2463
                     /locus_tag="VVN06_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5563347.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2486..3748
                     /locus_tag="VVN06_001859"
     CDS             2486..3748
                     /locus_tag="VVN06_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU5563348.1"
                     /translation="MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLFEALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKRYNGR"
     gene            3693..3905
                     /locus_tag="VVN06_001860"
     CDS             3693..3905
                     /locus_tag="VVN06_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEU5563349.1"
                     /translation="MCQPYQLTDIQKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKE
                     VMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT         1323 a          714 c          869 g         1083 t
ORIGIN      
        1 aaatttgaat atttaatagg tagacacttc aaaaaatgag gtgtctattt ttttacccga
       61 ttttgaaagg aagtgaactt atgaaaacaa aaaatcaaga atcaaaaggt cgttccccac
      121 tctttaagac catcaaacat tcattcagcc aataaaaaag aaaggatagg taaaaatatg
      181 gaacttaaat ttgtgattcc caacatggaa aaaacattcg gcaatttaga atttgctggc
      241 gaggataaag tcgttcagcg aagaatcaac ggacggctaa ctgtcttatc aagaagctat
      301 aatctctatt ctgatgttca aagagcagat gatattgtgg tggtgcttcc tgctgaagct
      361 ggcgaaaaac atttcggctt tgaggaacgt gtgaagttag tcaatccacg tattaccgca
      421 gagggctaca aaatcggcac tcgtggtttt acaaattacc ttttacatgc tgacgacatg
      481 ataaaagaat aaagaaagag aggaaaaatg atgagattag caaatggcat tgtattagat
      541 aaagacacga cttttggaga attgaaattc tctgctctac gtcgtgaagt gagaatccaa
      601 aatgaagacg ggtcggtttc agatgaaatc aaggaacgta cctatgactt aaaatccaaa
      661 ggacaaggac gcatgattca agtaagtatt cctgccagcg tgcctttgaa agagtttgat
      721 tataacgcac gggtggaact tatcaatccc attgcggaca ccgttgctac tgccacctat
      781 caaggagcag atgttgactg gtatatcaag gcagacgata ttgtgctgac aaaggattct
      841 agttcattca aagctcaacc acaagcaaag aaagaaccga cacaagacaa atagtcgcta
      901 ggtagaaagg agactttttc gcatgaaaca gcgtggtaaa aggattcgcc catctggtaa
      961 agatttagtc tttcatttta cgatagcgtc actcctgcct gttttcctgc tggttgtcgg
     1021 actgtttcat gtgaagacaa tccagcagat caactggcag gattttaacc tatcacaagc
     1081 agataagatt gacattccct atttaattat cagtttcagt gtcgcaattc ttatctgctt
     1141 gctggtagcg tttgtattca aacgggttcg ctatgatacg gttaaacaac tttaccaccg
     1201 tcaaaaactg gcaaagatga tacttgaaaa caagtggtat gaatctgaac aggtcaaaac
     1261 agagggtttc tttaaagata gtgctggtcg tacaaaggaa aagataacct acttccctaa
     1321 aatgtattat cgacttaaaa atggcttgat acagatacgg gtggaaatca cgctgggaaa
     1381 atatcaagac caactcttac acttggaaaa gaaattagag agtggcttgt actgtgagct
     1441 gacggataaa gagttaaagg attcctatgt ggaatatact ttgctctatg acaccatagc
     1501 cagtcgtatt tctattgatg aagtacaagc taaagatggt aaacttcgct taatgaaaaa
     1561 cgtatggtgg gaatatgata agctccctca tatgttgatt gctggtggta caggtggcgg
     1621 taaaacttac tttatactga cactgattga agccttgctt catacagatt caaaactgta
     1681 tattcttgac ccgaaaaatg ctgaccttgc ggacttaggt tctgtgatgg caaatgtcta
     1741 ctatagaaaa gaagacttgc tttcttgcat tgaaacattc tatgaagaaa tgatgaaacg
     1801 tagtgaggaa atgaagcaga tgaagaacta taagactggc aaaaattatg cttacttagg
     1861 tctcccggca cacttcttaa tctttgatga atacgtcgct ttcatggaaa tgctgggaac
     1921 aaaagaaaac accgcagtta tgaataagct gaaacagatt gtcatgttag gtcgtcaagc
     1981 tggcttcttt ctaatactgg cttgtcaacg tccagacgca aaatatttag gcgacggaat
     2041 ccgtgatcag tttaatttca gagtggcttt aggtcgtatg tctgaaatgg gctatggcat
     2101 gatgtttggc agtgacgtac aaaaggattt cttcttaaag cgaatcaaag gtcgtggcta
     2161 tgttgatgta ggaacaagtg tcatatcaga gttttatact ccccttgtac caaaaggata
     2221 tgatttcttg gaggaaatta aaaagttatc caacagcaga cagtccacgc aggcgacgtg
     2281 cgaagcggaa gtcgcaggtg tggactgatc ttgctggctg gtgtggcaat agccacgcca
     2341 gcacttaacc ccccgtatct aacagggggg tacaaatcga caggaaacag tcaaaaaaac
     2401 attagaaaat cctttggtta caagggattt acaaaatttc agcgtatgtc aaatgggctt
     2461 taaaagttga catacgcctt tttgattgga gggattttta ctgaatgaac aaacttggtt
     2521 acagcagtta aaagaaaaac gcttggctta tggactatct caaaatcgtt tagcggttgc
     2581 gactggtatt acaaggcaat atctaagcga tattgaaaca ggaaaagtca agccatcaga
     2641 ggatttacag caatctcttt ttgaagctct ggaacgcttc aatcccgacg ctccccttga
     2701 aatgctcttt gattatgtaa ggattcgctt tcccacaacg gacgtacagc atgtggtcga
     2761 aaacatctta caactgaaac tgtcctattt tcttcatgag gactatggtt tctattctta
     2821 ttcagagcat tatgctttag gcgatatatt cgttctctgc tcccacgaac tggacaaagg
     2881 agttctggtg gaattgaaag gtcgtgggtg tcggcaattt gaaagctatc ttctggctca
     2941 acaaagaagc tggtatgagt tctttatgga cgctttggtg gctggcggtg tgatgaaacg
     3001 ccttgacctt gccattaacg ataagacagg gattttgaat atccctgtac tcactgaaaa
     3061 gtgccaacag gaagaatgta tctccgtctt ccgcagtttt aaaagctatc gcagtggcga
     3121 actggtacgc aaagaggaaa aggaatgtat gggaaacacc ctctatatcg gttcattaca
     3181 aagtgaagtt tatttctgta tctatgaaaa ggactacgag cagtacaaga aaaatgatat
     3241 tcccattgaa gacgcagaag taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc
     3301 ctattatgca gtccgtgatt tactcgtcta tgacaatcca gagcataccg cctttaaaat
     3361 tatcaatcgg tatatccgtt ttgtagataa agacgattcc aaacctcgtt ctgattggaa
     3421 actgaatgaa gaatgggctt ggtttattgg gaacaatcgt gaacgattaa aactaaccac
     3481 aaaaccagag ccttactcct tccaaaggac gctgaactgg ctatctcatc aagttgcccc
     3541 gaccttaaag gttgcgatta aacttgatga aatcaaccag acgcaggttg taaaagacat
     3601 tctcgaccat gcgaaactga cagaccgaca caagcagatt ttgaagcaac agtcagtaaa
     3661 agaacaggac gtgataacaa caaaaaaagg atatctgtca accataccag ttgacagata
     3721 tccaaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
     3781 tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac
     3841 ttagaagcaa acttaagagt gtgttgatag tgcattatct taaaattttg tataatagga
     3901 attgaagtta aattagatgc taaaaatttg taattaagaa ggagggattc gtcatgttgg
     3961 tattccaaat gcgtaatgta gataaaaca
//
LOCUS       DAVHIU010000133         3869 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVHIU010000133 DAVHIU010000000
VERSION     DAVHIU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.135"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1398)
                     /locus_tag="VVN06_001861"
     CDS             complement(<1..1398)
                     /locus_tag="VVN06_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU5563350.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYSQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNK"
     gene            complement(1651..3384)
                     /gene="ptsP"
                     /locus_tag="VVN06_001862"
     CDS             complement(1651..3384)
                     /gene="ptsP"
                     /locus_tag="VVN06_001862"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU5563351.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQRAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(3390..3653)
                     /locus_tag="VVN06_001863"
     CDS             complement(3390..3653)
                     /locus_tag="VVN06_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU5563352.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1000 a          827 c          764 g         1278 t
ORIGIN      
        1 cttattgtaa aattctcgat cactagatgg gaggtcagtt ttcttagctc ctagcttatg
       61 agataaactt tcctgcttgg ccagtttgct atcaatgcct gctgctgttt ctgctgaaag
      121 atccttggct gggatataac gagaaactcc attctcctca aagacataac catcgcctac
      181 ttttcgaaca gcttctttga ccaatttctc atcaattgga ttgcttggag ctggttgagg
      241 atttggtgca ggttgcagac ttggactagg ttccggagtc gattgtggac ttggttgttc
      301 tggtcttgaa tctggtaccc aatggtttga acgataacga aggggaataa tacgagcaat
      361 tcgtttttcc aattcagaca tttgagagta agggataaag tggtaatggt taccatgagg
      421 gacagctaca cctctggcgg ttcgacttgt gatttgcgct gggtcgaaaa taaggccatc
      481 agattccaca tggcgttgac tcaaaggcag tttgtagagc tgtttcaaga gactatcaat
      541 gtcattactt tgacttgctt gactgttagt gttgctgttg ttgcttgtgt tagtatttgt
      601 agttcctgga ttgcttacag aaggtaccca gtttgttctt ggagtgttat cgctattttg
      661 tcggcgatag gttcttaaat ttgacagatt ttcccgacca gataggaagg cttctgcagc
      721 agccaactcg ctagctgata actcattctt aggaatgtaa tggtaatgat ctccatgagg
      781 aacgatataa gcatcaccag tgtcctcaat gatatcagat gcattgaaga tatacccatc
      841 atctgtggtg tagcgtccct gtgaacgtgc aaaggctacc gcaccatcgt ttgttgaagt
      901 ccctccttca cgatgctgac tatgttcttg tttttgtcga ttgatttcct cttttgtacg
      961 gacgttatcc gcgtgggcag catccttaag gtaaacatag tattttccat ctaccttgat
     1021 aacatatcca cccttgacct cattaacaat atcctcatct tttagcttat agtttggatc
     1081 tttcatgagt aattcttcac tgaaaatcgc atcataagga accttcccat tgtaataatg
     1141 atagtggtcg ccatgtgaag tgacatagcc ttggtctgtt atcttgatga cgatttgctc
     1201 agcattgatt ccttcacgct tgctaacctc atcaggagtc aagttctccg ttttttgcgt
     1261 cgcttgtttt ccatctatat aggaaacacg attattttcc ttaaccgttc tagcttgata
     1321 cagtcccaac tcgtaagaac aaacacttaa aatcaaagct gccgcagaac caacaaggta
     1381 tttcttatta attttcatcg aaactctctt tctatttgat atttctagtt gcaattcatt
     1441 tctaaattgc agaaactcct ctctattact taactggtta atagtttact cctaaatctc
     1501 caacttgtca agaattttat gaaccattta agtatttttt gttctgacca ctactttttc
     1561 aaggttggac tttctataag aaagattttc ttgaaaattt ttttaaaata tcaaaaaaat
     1621 ccctgtaact gagttgcagg gacttttcga ttaatcaaaa ttaacgtatt ctttttgaag
     1681 ttcaagaact tcttccattg ttgagcattc tgtaagggca cggtttgcgt actcttccat
     1741 cttagctgtg tcgagtttct tcatcaagct gcgtgtacga agtacagatg ttgctgacat
     1801 agagaactca tccaagccca ttccgacaag aagtggaaca gctcgttggt caccagccat
     1861 ctcaccacac ataccagccc atttaccttc agcgtgagct gctttgatca cattgttaat
     1921 caagcgtagg attgatgggt tgtatggttg gtaaaggtat gaaacttgtt cgttcatacg
     1981 gtctgctgcc attgtatatt ggatcaagtc gtttgtacca attgagaaga agtcaacttc
     2041 tttagcaaat tggtctgcaa gcatagccgc tgcaggaatc tcgatcatga taccaacttg
     2101 gatgttatcc gcaactgcaa caccttcagc aagaaggttt gctttttctt catcaaagac
     2161 tgctttcgct gcacggaatt ctttcaagag cgcaaccatt gggaacatga tacgcaattg
     2221 accgtgaaca gacgcacgaa gaagagcacg gatttgtgtg cggaacatag catctccagt
     2281 ctcagagata gagatacgaa gagcacggaa tccaaggaat gggttcattt cgtgaggcat
     2341 atcgaagtaa ggaagttcct tatctccacc gatatccatt gtacgaacga caacaggttt
     2401 accgttcatt ccttcaagaa cagccttgta tgcttcatac tgctcatctt cagttgggaa
     2461 gtcttgagaa tccatgtaca agaactctgt acggtaaagt ccaacagctt ctgcaccgtt
     2521 gttgttaaca ccttcaacgt cttttggagt accaatattg gcagccaact cgaagtgttt
     2581 accgtcagca gtcactgttt gagcatcttt caaaagtgcc cattcagctt tttgtttcgc
     2641 ataggcttca ccagctgctt taaattctgc cgcttgttca tctgttgggt tgataatcac
     2701 ttctccagtg atcccgttaa cagcaaggat gtcaccgtct ttaacgattt cagtgatgtt
     2761 gtttgtacct aatacagcag caatttcaag tgtacgtgcc atgatagctg agtggcttgt
     2821 acgtccaccg atgttggtta caaaagcttt tacaaagttt ttgtccaatt gagctgtatc
     2881 tgaaggagtc aagtcatgcg caatcacaat cacttcttca ttgatagaag ctgggtttgg
     2941 caatttttta ccaagaaggt ttgccaatac acgttttgtc acgtcgcgga tatccgctgc
     3001 gcgttcttgc atgtatgggt tgtcttccat gccttcaaag atagtgataa acatatctgt
     3061 aacttctttc agacctgctt ctgcattcac tttcttcgca cggatagttt ccttgatttg
     3121 gctgatcatt tctgggtcag caagaaccat taagtgagca tcaaaaactt gagctgcttc
     3181 ttcaccgagc gtacctactg ctttctcgcg aataacagaa agctcgtctt gtgatgcctg
     3241 tagagcggca tcaaggcgag cttcttctgc gtttgtatct tcgactgtaa tagtctcaaa
     3301 tgacaaatcc ggctgaacga gtagatatgc ttttgcaact gcaacaccgt cagatgctgc
     3361 gattccttta agcatttctg tcatttccct tatgccaatc cttctttttc cattgtttct
     3421 gagattgcag cgatagcgtc atctgcatct gcaccttcag ctgagatagt tacgtcagca
     3481 ccttggccaa caccaagact cataacaccc ataattgatt taaggttaac tgatttacct
     3541 ttgtactcaa gagtgatatc tgaagcaaat ttgctagcag tttgtaccaa caatgttgct
     3601 ggacgtgcgt gaatacctgt ttctgccact acgtggaaat ctttagaagc catagtttga
     3661 ctctcctttt gttctttctt tttttgagtt atatgtgata acccttacaa tttagtatta
     3721 taccatcttc taggattttt ttcaagcatt ttctggattt tccttcaatt tcctttcaga
     3781 taacttgctg aaaatcttct atcctagata acaaaacaca ggatatagtt tctttaaaaa
     3841 caatttatag gataatcatt gctatttca
//
LOCUS       DAVHIU010000134         3796 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVHIU010000134 DAVHIU010000000
VERSION     DAVHIU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.136"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..2176
                     /gene="mfd"
                     /locus_tag="VVN06_001864"
     CDS             <1..2176
                     /gene="mfd"
                     /locus_tag="VVN06_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5563353.1"
                     /translation="HGFHFVDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNEL
                     EKGDYVVHHIHGIGQYLGIETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISS
                     DGKAPKLNKLNDGHFKKAKQKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAF
                     DDAFPYVETDDQLRSIEEIKRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQ
                     VVILVPTTVLAQQHYTNFKERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIG
                     THRVLSKDVVFADLGLMIIDEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSM
                     LGIRDLSVIETPPTNRYPVQTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQK
                     VSELQELIPEASIGYVHGRMSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTL
                     FIENADHMGLSTLYQLRGRVGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGS
                     GFKIAMRDLSIRGAGNLLGKSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGN
                     AELILQIDAYLPDTYISDQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAY
                     LLEIGLVKSYLDKVFVQRVERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAE
                     NKELMELVFDVQNKKDYEILEGLLIFGESLLEIKESKEENSI"
     gene            2234..2500
                     /locus_tag="VVN06_001865"
     CDS             2234..2500
                     /locus_tag="VVN06_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU5563354.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            2493..2861
                     /locus_tag="VVN06_001866"
     CDS             2493..2861
                     /locus_tag="VVN06_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5563355.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            2981..>3796
                     /locus_tag="VVN06_001867"
     CDS             2981..>3796
                     /locus_tag="VVN06_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5563356.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGELTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLP"
BASE COUNT         1310 a          546 c          791 g         1149 t
ORIGIN      
        1 acatggtttt cattttgtag atgaaaagat tttattgata actgaacatg agatttttca
       61 aaagaaatta aagcgtcgtt ttcgaagaca acatgtttca aatgcagaga gattaaaaga
      121 ttacaatgaa cttgaaaaag gggactatgt tgtccatcat atccatggga ttggtcaata
      181 tctaggaatt gaaaccattg aaatcaaggg aattcatcgt gattatgtca gtgtccaata
      241 ccaaaatggt gatcaaattt ctattcccgt ggaacagatt catctactgt ccaaatatat
      301 ttcaagtgat ggtaaagctc caaaactcaa taaattaaat gacggtcatt ttaaaaaggc
      361 caagcaaaag gttaagaacc aggtagagga tatagctgat gatttaatca aactctactc
      421 tgaacgtagt cagttgaagg gttttgcttt ctcagctgat gatgatgatc aagatgcctt
      481 tgatgatgct ttcccttatg ttgaaacgga tgatcaactt cgtagtattg aggaaatcaa
      541 gagggatatg caggcttctc agccaatgga tcgactttta gttggggatg ttggttttgg
      601 aaagactgaa gttgctatgc gtgcagcctt taaagcagtc aatgatcaca aacaggttgt
      661 cattctagtt ccgacgacgg ttttagcgca acagcactat acgaatttta aggaacgatt
      721 ccaaaatttt gcagttaata ttgatgtgtt gagtcgcttt agaagtaaaa aagagcagac
      781 tgcaacactt gaaaaattga aaaacggtca agtcgatatt ttgattggaa cacatcgtgt
      841 tttgtcaaaa gatgttgtgt ttgctgattt gggcttgatg attattgatg aggaacagcg
      901 atttggtgtc aagcataagg aaactttgaa agaactgaag aaacaagtgg atgtcctaac
      961 cttgaccgct acgccaatcc ctcgtaccct ccatatgtct atgctgggaa tcagagattt
     1021 atctgttatt gaaactccgc cgactaatcg ctatcctgtt cagacctatg ttttggaaaa
     1081 gaatgatagt gtcattcgtg atgctgtctt gcgtgaaatg gagcgtggag gtcaagttta
     1141 ttatctttac aacaaagttg acacaattgt tcagaaggtt tcagaattac aggagttgat
     1201 tccggaggct tcgattggat atgttcatgg tcgaatgagt gaagtccagt tggaaaatac
     1261 tctattagac tttattgagg gacaatacga tatcttggtg acgactacta ttattgagac
     1321 aggggtggac attccaaatg ctaatacttt atttattgaa aatgcggacc atatgggctt
     1381 gtcaacctta tatcagttaa gaggaagagt cggtcgtagt aatcgtattg cttatgctta
     1441 tctcatgtat cgtccagaaa aatcaatcag tgaagtctct gaaaagagat tagaagcgat
     1501 taaaggattt acagaattgg gctctggctt taagattgca atgcgagatc tttcgattcg
     1561 tggagcagga aatcttttag gaaaatccca gtctggtttc attgattctg ttggttttga
     1621 attgtattcg cagttattag aggaagctat tgctaaacga aacggtaatg ctaacgctaa
     1681 cacaagaacc aaagggaatg ctgagttgat tttgcaaatt gatgcctatc ttcctgatac
     1741 ttatatttct gatcaacgac ataagattga aatttacaag aaaattcgtc aaattgacaa
     1801 ccgtgtcaat tatgaagagt tacaagagga gttgatagac cgttttggag aatacccaga
     1861 tgtagtagcc tatcttttag agattggttt ggtcaaatca tacttggaca aggtctttgt
     1921 tcaacgtgtg gaaagaaaag ataataaaat tacaattcaa tttgaaaaag tcactcaacg
     1981 actgttttta gctcaagatt attttaaagc tttatccgta acgaacttaa aagcaggcat
     2041 cgctgagaat aaggaattaa tggagcttgt atttgatgtt caaaataaga aagattatga
     2101 aattttagaa ggtctgctga tttttggaga aagtttatta gagataaaag agtctaagga
     2161 agaaaattcc atttgatatt tttcttctat aaaatagata aaaatggtac aataataaat
     2221 tgaggtaata aggatgagat tagataaata tttaaaagta tcgcgaatta tcaagcgtcg
     2281 tacagtcgca aaggaagtag cagataaagg tagaatcaag gttaatggaa tcttggccaa
     2341 aagttcaacg gacttgaaag ttaatgacca agttgaaatt cgctttggca ataagttgct
     2401 gcttgtaaaa gtactagaga tgaaagatag tacaaaaaaa gaagatgcag caggaatgta
     2461 tgaaattatc agtgaaacac gggtagaaga aaatgtctaa aaatattgta caattgaata
     2521 attcttttat tcaaaatgaa taccaacgtc gtcgctacct gatgaaagaa cgacaaaaac
     2581 ggaatcgttt tatgggaggg gtattgattt tgattatgct attatttatc ttgccaactt
     2641 ttaatttagc gcagagttat cagcaattac tccaaagacg tcagcaatta gcagacttgc
     2701 aaactcagta tcaaactttg agtgatgaaa aggataagga gacagcattt gctaccaagt
     2761 tgaaagatga agattatgct gctaaatata cacgagcgaa gtactattat tctaagtcga
     2821 gggaaaaagt ttatacgatt cctgacttgc ttcaaaggtg ataaaatgga aaatttatta
     2881 gacgtaatag agcaattttt gggtttgtca gatgaaaagt tggaagaatt ggctgataaa
     2941 aatcaattat tgcgtttaca agaagaaaag gaaaggaaga atgcgtaaat tcttaattat
     3001 tttgttgcta ccaagttttt tgaccatttc aaaagtcgtt agcacagaaa aagaagtcgt
     3061 ctatacttcg aaagaaattt attacctttc acaatctgac tttggtattt attttagaga
     3121 aaaattaagt tctcccatgg tttatggaga ggttcctgtt tatgcgaatg aagatttagt
     3181 agtggaatct ggggaattga ctcccaaaac aagttttcaa ataaccgagt ggcgcttaaa
     3241 taaacaaggg attccagtat ttaagctatc aaatcatcaa tttatagctg cggacaaacg
     3301 atttttatat gatcaatcag aggtaactcc aacaataaaa aaagtatggt tagaatctga
     3361 ctttaaactg tacaatagtc cttatgattt aaaagaagtg aaatcatcct tatcagctta
     3421 ttcgcaagta tcaatcgaca agaccatgtt tgtagaagga agagaatttc tacatattga
     3481 tcaggctgga tgggtagcta aagaatcaac ttctgaagaa gataatcgga tgagtaaagt
     3541 tcaagaaatg ttatctgaaa aatatcagaa agattctttc tctatttatg ttaagcaact
     3601 gactactgga aaagaagctg gtatcaatca agatgaaaag atgtatgcag ccagcgtttt
     3661 gaaactctcc tatctctatt atacgcaaga aaaaataaat gagggtcttt atcagttaga
     3721 tacgactgta aaatacgtat ctgcagtcaa tgattttcca ggttcttata aaccagaggg
     3781 aagtggtagt cttcct
//
LOCUS       DAVHIU010000135         3744 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVHIU010000135 DAVHIU010000000
VERSION     DAVHIU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.137"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(118..1080)
                     /gene="nrdF"
                     /locus_tag="VVN06_001868"
     CDS             complement(118..1080)
                     /gene="nrdF"
                     /locus_tag="VVN06_001868"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU5563357.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADD
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD"
     gene            complement(1268..3427)
                     /gene="nrdE"
                     /locus_tag="VVN06_001869"
     CDS             complement(1268..3427)
                     /gene="nrdE"
                     /locus_tag="VVN06_001869"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU5563358.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3508..3726)
                     /gene="nrdH"
                     /locus_tag="VVN06_001870"
     CDS             complement(3508..3726)
                     /gene="nrdH"
                     /locus_tag="VVN06_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU5563359.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1076 a          693 c          804 g         1171 t
ORIGIN      
        1 acaaaatgaa acaaaaacaa aaatatcgaa gtttgttttc aaaactttcg atatttttgt
       61 tgggttataa ctttgatgtt tctagtttac tttttgatga ttgagagtgg tggagaatta
      121 gtctaaaccg tagttatagt catcgtcttg catgacttca acttcgccaa gaaggtaacc
      181 atttccgact tgagagaaga agtcgtggtt ggaagttcct gttgaaatac cgttcattac
      241 gattgggttg acatcatcag ctgaatctgg gaagagtgga tcttgtccca tgttcatgag
      301 agctttattg gcattgtagc gaaggaaggt tttaacctct tcagtccaac caacaccgtc
      361 ataaagactc tctgtgtagc cttcttcatt ttcataaaga gtatagagta ggtcgtacat
      421 ccattctttg agtttttctt gctcttcttc aggtaattca ttgaaaccaa gttggaattt
      481 gtaaccaatg taggttccgt gaacagactc gtcacgaata atcaatttaa tgatttctgc
      541 aacgttggca agtttgttgt taccgagata gtagagggga gtgaagaaac cagagtagaa
      601 gaggaaggtt tcgaggaaga cgctggcaac tttcttttca agtgggctgc cgtttaggta
      661 gatttcgttg acaatctcag ccttcttttg taggtaagga ttggtattgg tccattcgaa
      721 aatttcttca atctcagcct tagtattcaa ggtagaaaag attgatgagt aagatttagc
      781 gtggacagat tccataaatt ggatgttatt gaaaacagct tcctcatgtg gtgtacggat
      841 gtctgcgcga agggcttgaa ccccagtttc agattgcata gtgtcaagaa gggttaaacc
      901 accaaaaact tttccgacca agtctttctc tttgttagat agctttctcc agtcatccaa
      961 gtcgtttgat aagggaatac gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt
     1021 tgatttgtcg atgacatctt cgatggcatt ccagttaatg gctttgtagt aagtttccat
     1081 ttaaaatctc tttctgtgtt tagtattgcg aactcacaat tatttctact ttaccataat
     1141 tctataggag tatcgcacaa aaagtcggaa gcccgacttt taaaatgtta cataaattat
     1201 gttatgacat agtagatttg attttatcag tgctgcttag ggaaaaatag tgtttctatg
     1261 ctagaaacta aatcacacag ctttcacatt ggttggcgcc gacttctcca ccgtcatctg
     1321 taaaggtacg gacgtagtag atagacttga ttcccttgtt aaaggcatag ttacgaagga
     1381 tggacaagtc acgtgtcgtt tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa
     1441 tgtcactgcg catgaagagg gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag
     1501 cgtaaacatc gatgacttta cgcatatcca tatcgtaggc agaagtgtag taaggaatgg
     1561 tttctgtaga caagccagca gcagggtagt agattttacc aattttcttc tcttggcgtt
     1621 cttcgatacg ttgcgtaatc gggtggatag aagcagaaac gtcgttgata tagctgatag
     1681 aaccatttgg cgctacagca aggcgatttt ggtggtaaag accatcttct tgaaccttgt
     1741 cgcgaagttc agcccaatca gcaacaccag ggataaaaac atttttgaag agttctttaa
     1801 cacggtctga tgttggaaca aattcaccag ttacatactt gtcaaagtaa cttccgttag
     1861 catagtctga tttttcaaag ttgtggaagg taataccacg ttcacgcgca atattgtttg
     1921 actctaccaa ggtccagtag ttcataagca taaagtagat gcttgtaaat tcaacagact
     1981 caggtgaacc atattcaatg agttgttggg caaggtagct gtgcagtccc atggcaccga
     2041 gaccaaaggt gtgggcttgg ctatttccat ggtcaatcgt tggtacagct acgatatgtg
     2101 aactatctgt aacgaaagta agggcacgaa ccatagcacg gatagaacga ccaaaatcag
     2161 gtgaagtcat catgttaacc acgttggttg aacccagatt acatgaaaca tctgttccca
     2221 tttgaaggaa ttcttgagca tcgttgatca agcttggttc ttgaacttga agaatctcag
     2281 aacacaagtt actcatgata atctttccat caacaggatt tgcacggtta gctgtatcga
     2341 tgttgactac ataaggatag ccagactctt gttgcaattt agagatttca gtttccaaat
     2401 cccgcgcctt gatttttgtc ttgcgaatat ttggatttgc gaccaattca tcgtattttt
     2461 cagtaatgtc gatgtaattg aatggcacac cgtattcttt ttctacagag taagggctga
     2521 agaggtacat ttcttcattt ttacgagcca attcgtagaa tttatcaggt actacaacac
     2581 caagtgatag agtcttgaca cgtacttttt catcagcgtt ttctttctta gttgaaagga
     2641 aagcgatgat atctgggtga aagacgttga ggtagacaac accggcacct tgacgttgcc
     2701 ccaattggtt ggagtaagag aagctgtctt caaaaagctt cataacagga acgacacctg
     2761 aagcagctcc ttcatagcct ttgataggtg caccagcttc acgaaggttg ctgagggtaa
     2821 ttcccacacc accaccaata cgtgaaagtt gaagagctga gttgatagaa cgtccgatag
     2881 agttcatatc atccgtcact tggattagga aacaagatac caactcccca cgacgagcac
     2941 gtccagcatt caagaaggaa ggagtagcag gttggtagcg ttggtggatg atttcattgg
     3001 caatatcgat tgcaacagct tcattcccat cagcgaaata aagggcgttg aagaagacac
     3061 ggtcttccat attttcaaga tagtattcac cgtcattagt ctttaaggca tattgattgt
     3121 aaaatttata agctgccatg aatgacttga attggaagtt ttggtctttg ataaattgag
     3181 ccaattcttc caagaactct ggacggtatt tcttgataaa ggctgtttcg atgtagttgt
     3241 gttcaatgag gtaattgatt ttgtctttga ttgaatcaaa aaccatagtg tttggaacta
     3301 cattttcttt aaagaaagca tccaaggctt ccttgtcttt atgaagcatg atttgtccat
     3361 taacaggacg gttaatttcg ttattaagac ggaagtaagt cacgtcttca agatgtttta
     3421 atcccataaa atttccctta tctaattaca aaagaaaggc ttctaagtta gccctaaaag
     3481 cagtttcttc tggatgatgt actaagatta tgctaattgt ttcagttttc ctggttggaa
     3541 acctgaaaag acttcagttg gtgtttggat aacaggagct gcgctaaaac cgagctcttt
     3601 aacttgatcg acgtactcag gttgctcatc aagattgatt tcacgataag agacattatt
     3661 actgtccaag aaacgcttgg tcattttaca ttggacacaa ttgtttttag aataaacggt
     3721 taccattgtg taactcctct tcaa
//
LOCUS       DAVHIU010000136         3736 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVHIU010000136 DAVHIU010000000
VERSION     DAVHIU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.138"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(493..690)
                     /locus_tag="VVN06_001871"
     CDS             complement(493..690)
                     /locus_tag="VVN06_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563360.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
     gene            complement(675..893)
                     /locus_tag="VVN06_001872"
     CDS             complement(675..893)
                     /locus_tag="VVN06_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU5563361.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(991..1776)
                     /locus_tag="VVN06_001873"
     CDS             complement(991..1776)
                     /locus_tag="VVN06_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563362.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1778..2596)
                     /locus_tag="VVN06_001874"
     CDS             complement(1778..2596)
                     /locus_tag="VVN06_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5563363.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2598..3590)
                     /locus_tag="VVN06_001875"
     CDS             complement(2598..3590)
                     /locus_tag="VVN06_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5563364.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1250 a          811 c          685 g          990 t
ORIGIN      
        1 caaagagtaa actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga
       61 tggaactgac gtagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt
      121 cagtaaccat acgcacggca aggcgacgtt gacgtggttt gaagagattt tcgaagagta
      181 taagtccaaa actttcgtta accgggatac attataagca cattcatacc atcaaaaata
      241 gtagcaataa acttgaacaa ctaccagaaa cacgaaaaca ggcataattg atgaaatatc
      301 agcgctatta aacaatataa ataaccgcta agagtaaggc ttaacggtta tcttctacct
      361 atctattttt tcaaatcctc agtcgtttat ctacctataa cctaggcttt acgagtgcct
      421 tgctctacca actaagctaa atcggcgatt acacttacag tatagcactc tgagaataga
      481 tgtcaagtaa tattattcaa gctttttaat caaaatctga caaggatttt gcggattcca
      541 tagttgaaga aaaatctcta actttttaaa gccaagacct cgataaaagt catttgttcg
      601 atcataatct ttattagaac cttctgccac tgttttgacc tgcagataac caactttttt
      661 acgagcttca ctctctaaag tagcaagcaa ttggctccca atttttctac cttgataagc
      721 ttttttacgc cgagacaatc aatctctgca caatcttcac tcgaatagga taaccttaca
      781 aatccagtca aatcactctc ctgataggcg gtccaaactt gcagtgtcgt ggttccttct
      841 atataggctt gtgtgctttc tgggattcca aaccattctg gcaaatcctt caaaacctca
      901 acgacaactg ccttttttga ttttcatcct tgacttcttc aattacaaac atcataagtt
      961 cctttcttga ttttagtctt actttagctt ttacgaaccc gcactttcgt aggaatctaa
     1021 gcccttatcc caaagtttaa gggaaataac aaagaaaacc agagaaatca acatcaaacc
     1081 tcctacgtta aagaacacat ccttttcctg taagaaatag ctagctggat agtaggctgt
     1141 aaaggcgaaa ggcacgataa agctaatcaa ccaacgaaga agagaattgt aaatagaaat
     1201 cggatactta gcaaagtcat tgaacatata gaagatgtaa atcatggcgc ctgactgctt
     1261 agtccaaaag gcgatactgg ctgttgcgat tttaagagaa gtataaatca aggtcgcaaa
     1321 aggaatacaa actaggaaaa gcaggaattt tggaagagtc caaacaatgc tggtcactgt
     1381 tgttcccaat aaaataccac cgactaagag ttcacccaag gcatcaatct gaaaggtttc
     1441 aactaggatg tgaaagagag gattgatggg acgagtcaga tacttgtcaa actctccttt
     1501 tcggactagg cgttgcccta gtgcccagag attgtcaaaa aagagatggt ccattccctt
     1561 gggaatcaag gaaaatccat aaatgaaagc tatctcttga aaggtccagc cttctaggaa
     1621 tggaatatgt tgaaagatga cattgagaaa caagagattc aagccttgag ccagaaagac
     1681 tcccaagaca ccaaccacaa aatccacctt atattccatg atttgtttga tgtattgtct
     1741 gataaaaatc agatgcattc gttgatattt tttcatacta acctccttga atggtgataa
     1801 aggactggac ccgtttccaa attaactgag acaatcccac catcactaag agccagaaga
     1861 actgcaaaag gagtgcctga agaatctgac tggcatcgta ttttccaaca atgatcataa
     1921 ctggagtata aatcaaggac gaaaaaggca aaaaggagag aatatctgaa acaacctttg
     1981 gaaagaatgc caagggaatc aaactccccg acataaaagc cactatggaa gtcttaagta
     2041 ggttggaacc ccaaagattt ttaaacacaa aggctgaaaa tccaaagcaa atattaaaga
     2101 aaaagttaat cagataggcg agcgttaagc taaaaagata aaggacagtt aatcctaaca
     2161 cctctacaat accttgaccc gatatgattt tcatcaagac aatgacactt aaaaatggaa
     2221 ggccaacgct gataaaaatc aaccacttgg aaccaagctc ggtgaaaaga taggaggccg
     2281 caaaatgcac tggtcgcaac aaacgcatga taatggagcc atccttgacc tcctccccaa
     2341 tcataaagga cgaatcggat ctagtcagaa gattggtcac aaaactcatg atgatgtaga
     2401 gggtgatatc cgccatactg aagccctgaa tcaaagactc ttgcgaagaa tcaaagacag
     2461 ccttccagag ataaaaggcc acaaaagccc ccatgacatc gccaatccga tagagaataa
     2521 agttgactcg gtaagtaatc aactcctgaa cccctgcatt gataaagggt ttataacgtc
     2581 tccacaattt gatcatccta gagctccttt cggtagaagc gacggataat atcctcaata
     2641 tccgtatcca ccatcttcaa atcgcggatt tcaaaatcag atagggtttg cttgataatg
     2701 tcagctgact ggtagcgaga actatcaaat tcaatgttga ggctgtttcc ttgtctatca
     2761 atggtcatat cagacagacc ttcatagtga gagacgagat gactttgacc tggtagcagt
     2821 tcaaaagaga gagtcttcat cttaccaaag gtctccttga gttggctcac cgttccatca
     2881 aaaatctttt gccccttgtc aatcatgaaa atccgatcac aaagttgctc aatatcactc
     2941 aaatcgtgag tggtcaaaag aatggtagtt tcttcctctt gattgatctg agtaattgcc
     3001 cgacgaatat tatccttaac cgaaacgtcc aaaccaatgg tcggctcatc taaaaaaaga
     3061 accttgggat tgtggagcaa ggaggccgca atatccgctc gcatccgttg tcccagtgaa
     3121 agagtccgca cgggatcctt gataaagtcc ttcaaatcca agacttcatt caaaaagtcc
     3181 atacgcttat gaaagagcga gtttggcaca tcataaatct cttttaagac agtgtaggtc
     3241 tcttgcagag ccaaatccca ccatagctgg gtgcgttgtc caaagactac gccaatatct
     3301 ttgacataat cttgccgatt gtcctgggga atcttgccgt taatccgaca aaaaccagat
     3361 gttggtttca aaattcctgt cagcatttta atggttgtcg acttcccagc accatttgcc
     3421 ccgataaatc ctaaaatctg cccttttgga acctcaaagg tcaaatcctt gaccgcttca
     3481 aaggtctgct tttcaggatg aataaaggag cgcaaagccc ccttcaagcc cggttcctta
     3541 acaatcttca caaaatgttt ctgaagatgt tccacttcta tcattgccat atctatctcc
     3601 ctatcgtaag gaatagcaac attgtgtttt taactacaaa tattctaaat ccttactttc
     3661 tacaccttct acattttatt actacagaag gctttatacc aacctattat agtccaataa
     3721 aaaacgaaat gcaagc
//
LOCUS       DAVHIU010000137         3646 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVHIU010000137 DAVHIU010000000
VERSION     DAVHIU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.139"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            2..1447
                     /gene="cps4A"
                     /locus_tag="VVN06_001876"
     CDS             2..1447
                     /gene="cps4A"
                     /locus_tag="VVN06_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU5563365.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDMMEGR"
     gene            1449..2180
                     /locus_tag="VVN06_001877"
     CDS             1449..2180
                     /locus_tag="VVN06_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HEU5563366.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2189..2881
                     /gene="cpsC"
                     /locus_tag="VVN06_001878"
     CDS             2189..2881
                     /gene="cpsC"
                     /locus_tag="VVN06_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU5563367.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRDQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLQMT
                     LLGIVPNLNKLK"
     gene            2891..3574
                     /gene="cpsD"
                     /locus_tag="VVN06_001879"
     CDS             2891..3574
                     /gene="cpsD"
                     /locus_tag="VVN06_001879"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU5563368.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVFNKLDISVDKYGVY
                     GFYGNYGKK"
BASE COUNT         1173 a          585 c          773 g         1115 t
ORIGIN      
        1 catgagtaga cgttttaaaa aatcacgttc acagaaagtg aagcgaagtg ttaatatcgt
       61 tttgctgact atttatttat tgttagtttg ttttttattg ttcttaatct ttaagtacaa
      121 tatccttgct tttagatatc ttaacctagt ggtaactgcg ttagtcctac tagttgcctt
      181 ggtagggcta ctcttgatta tctataaaaa agctgaaaag tttactattt ttctgttggt
      241 gttctctatc cttgtcagct ctgtgtcgct ctttgcagta cagcagtttg ttggactgac
      301 caatcgttta aatgcgactt ctaattactc agaatattca atcagtgtcg ctgttttagc
      361 agatagtgat atcgaaaatg ttacgcaact gacgagtgtg acagcaccga ctgggactga
      421 taatgaaaat attcaaaaac tactagctga tattaagtca agtcagaata ccgatttgac
      481 ggtcaaccag agttcgtctt acttggcagc ttacaagagt ttgattgcag gggagactaa
      541 ggccattgtc ctaaatagtg tctttgaaaa catcatcgag tcagagtatc cagactacgc
      601 atcgaagata aaaaagattt ataccaaggg attcactaaa aaagtagaag ctcctaagac
      661 gtctaagaat cagtctttca atatctatgt tagtggaatt gacacctatg gtcctattag
      721 ttcggtgtcg cgatcagatg tcaatatcct gatgactgtc aatcgagata ccaagaaaat
      781 cctcttgacc acaacgccac gtgatgccta tgtaccaatc gcagatggtg gaaataatca
      841 aaaggataaa ttaacccatg caggcattta tggagttgat tcgtccattc acaccttaga
      901 aaatctctat ggagtggata tcaattacta tgtgcgattg aacttcactt ctttcttgaa
      961 aatgattgac ttattgggag gggtagatgt tcataatgat caagagtttt cagctctaca
     1021 tgggaagttc catttcccag tagggaatgt ccatctagac tctgagcagg ctctaggttt
     1081 tgtacgtgaa cgctactcac tagccgatgg agaccgtgac cgtggtcgca accaacaaaa
     1141 ggtcattgta gcaattattc agaagttaac ttctacagag gttttgaaaa actatagtag
     1201 tattcttcaa ggattgcagg attctcttca aacaaatatg ccgattgaga ctatgataga
     1261 tttagtgaat actcagttgg aaagtggggg gaattataaa gtaaattctc aagatttaaa
     1321 agggacaggt cggatggatc ttccttctta tgcaatgcca gacagtaacc tctatgtgat
     1381 ggaaatagat gatagtagtt tagctgtagt taaagcagct atacaggata tgatggaggg
     1441 tagatgaaat gatagacatc cattcgcata tcgtttttga tgtagatgac ggtcccaagt
     1501 caagagagga aagcaaggct ctcttggcag aatcctacag gcagggggtg cgaaccattg
     1561 tctctacctc tcaccgtcgc aagggcatgt ttgaaactcc ggaagagaag atagcagaaa
     1621 actttcttca ggttcgggaa attgcaaaag aagtggcaga tgatttagtc attgcttatg
     1681 gcgcagagat atactatact ctggatgctc tagaaaagct agaaaaaaaa gaaattccta
     1741 cccttaatga tagtcgttat gccttgattg agtttagcat gcatacttcc tatcgtcaga
     1801 ttcatacggg attgagcaat attttgatgt tgggaatcac gccagtaatt gctcatattg
     1861 aacgttatga tgctttagag aataacgaaa aacgtgttcg tgaactgatt gatatggggt
     1921 gctatactca gataaatagt tatcatgttt cgaaacctaa gttctttggt gaaaaatata
     1981 aattcatgaa aaagagagct cggtattttt tggaacgtga tttagttcat gtagttgcaa
     2041 gtgacatgca caatttagac agtagacctc catatatgca acaggcatat gatatcattg
     2101 ctaagaaata tggagcgaaa aaagcaaaag aactttttgt agataatccc agaaaaatta
     2161 taatggatca attaatttag gagaaaatat gaaggaacaa aacactttgg aaatcgatgt
     2221 attgcaacta ttcagagctt tatggaaaag aaagttggtc attttattag tggcaattat
     2281 aacttcttca gttgcttttg cctacagtac ttttgttatc aaacctgagt ttactagtat
     2341 gactcggatt tatgtagtta accgtgatca gggagagaag tctggtttaa ccaatcaaga
     2401 cttgcaggca ggatcatcct tggttaaaga ctatcgtgaa attatcctat cgcaggatgt
     2461 tttggaggaa gttgtttctg atttgaaact agatttgacg ccaaaagatt tggctaataa
     2521 aattaaagta acagtaccag ttgatacccg tattgtctct gtttcagtta gtgatcgagt
     2581 tcctgaagag gcaagccgta tcgctaactc tttgagagaa gtagctgctc aaaaaattat
     2641 cagtattact cgtgtttctg atgtgacaac actggaggag gcaagaccgg cgacatcacc
     2701 gtcttcgcca aatattaaac gcagtacact aattggtttt ttggcaggag tgattggaac
     2761 tagtgttata gttcttattc ttgaactttt ggacactcgt gtgaaacgtc cgaaagatat
     2821 cgaagataca ctgcagatga cacttttggg aattgtacca aacttgaata agttgaaata
     2881 ggagagagga atgccgacat tagaaatagc acaaaaaaaa ctggagttcg ttaagaaggc
     2941 agaagaatat tacaatgcct tgtgtacaaa tatacagttg agcggagata aactaaaagt
     3001 aatttccgtt acttctgtta accctgggga aggaaaaaca actacttccg taaatatagc
     3061 aaggtcgttt gcgcgtgcag gctataaaac tcttttgatc gatggcgata ttcgaaattc
     3121 agttatgtca ggatttttta aatctcgtga aaaaattaca gggctaacgg aatttttatc
     3181 tgggacagct gatttatctc acggtttatg tgatacaaat attgaaaatt tatttgtagt
     3241 tcaatcgggc actgtatcac caaaccctac agccttgtta caaagtaaaa attttaatga
     3301 tatgattgaa acattgcgta aatattttga ttatataatt gttgatacag cacctattgg
     3361 aattgttatt gatgcggcaa ttatcactca aaagtgtgat gcgtccatct tggtaacagc
     3421 aacaggtgag gtgaataaac gtgatgtcca aaaagcgaaa caacaattag aacaaacagg
     3481 gaaactgttc ctaggagttg tttttaataa attagatatc tcggttgata agtatggagt
     3541 ttacggtttc tatggaaatt atggtaaaaa ataatttagg aaagattcta tggatgaaaa
     3601 aggattgaaa atttttttgg cagtattaca gagtattatt gtcatt
//
LOCUS       DAVHIU010000138         3618 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVHIU010000138 DAVHIU010000000
VERSION     DAVHIU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.140"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            141..1370
                     /gene="arcA"
                     /locus_tag="VVN06_001880"
     CDS             141..1370
                     /gene="arcA"
                     /locus_tag="VVN06_001880"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU5563369.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1428..2444
                     /gene="argF"
                     /locus_tag="VVN06_001881"
     CDS             1428..2444
                     /gene="argF"
                     /locus_tag="VVN06_001881"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5563370.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2609..3556
                     /gene="arcC"
                     /locus_tag="VVN06_001882"
     CDS             2609..3556
                     /gene="arcC"
                     /locus_tag="VVN06_001882"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU5563371.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1145 a          708 c          757 g         1008 t
ORIGIN      
        1 taaaattgtg ctaaatatca taatttatat tattttccga gaaattatag ccattgacat
       61 tacctaggaa ttgcgttata acatacatgt aagcggtacc caaaatggtg ccaagtcaaa
      121 atttttaagg aggaaaatac atgtcttcac atccaattca ggtcttctca gaaattggga
      181 aactgaaaaa agttatgttg caccgtccag gcaaggagtt agaaaacttg ttgccggact
      241 atcttgaaag gcttcttttt gatgatattc ctttcttgga agatgctcaa aaagaacatg
      301 atgcatttgc ccaagctctt cgcgatgaag gaattgaggt tctctaccta gaacaactcg
      361 ctgctgaatc attgacctct ccagaaatcc gcgatcaatt tatcgaggaa tacttagacg
      421 aagccaacat ccgtgatcgt caaaccaagg ttgctattcg tgaattgctt cacggcatca
      481 aggacaacca agaattggtt gaaaaaacaa tggctgggat tcaaaaagtt gaattgccag
      541 aaattcctga cgaagctaaa gatctaactg acttagttga atcagattat ccatttgcaa
      601 ttgacccgat gccaaacctc tatttcactc gcgacccatt tgcaacaatt ggaaacgccg
      661 tatcgcttaa ccacatgttt gcagacactc gtaaccgtga aacactctac ggtaagtata
      721 tcttcaaata ccacccaatc tatggcggaa aagtggattt ggtctacaac cgtgaagaag
      781 atacgcgtat cgaaggtgga gacgagctag ttctttctaa agacgtcctt gcagtaggta
      841 tctctcaacg tacagacgca gcttctatcg aaaaactttt ggtcaacatc ttcaagaaaa
      901 atgttggctt caagaaagtt ttggcctttg aatttgctaa caaccgtaaa ttcatgcact
      961 tggatactgt cttcactatg gtagactatg acaagttcac tattcaccca gaaatcgaag
     1021 gcgaccttca cgtttactca gttacttacg aaaacgaaaa acttaaaatc gttgaagaga
     1081 aaggtgactt agctgaactt cttgctcaaa accttggtgt agaaaaagtt catttgattc
     1141 gttgcggtgg tggcaatatc gtagcagctg cgcgtgaaca atggaacgac ggttctaaca
     1201 ctttgaccat cgcacctggt gtggtagttg tttatgaccg caataccgtg accaataaga
     1261 ttttggaaga atacgggctt cgcttgatta agattcgcgg aagtgaattg gttcggggcc
     1321 gtggtggacc tcgttgtatg tctatgccat ttgaacgtga agaagtgtaa tcgctgttcg
     1381 gtattcgtca atagaaaatg taaaaaatag aaagaggaaa taataaaatg acaaattcag
     1441 tattccaagg acgcagcttc ttagcagaaa aagactttac ccgtgcagag ttagaatacc
     1501 ttattggtct ttcagctcac ttgaaagatt tgaaaaaacg caatattcaa caccactacc
     1561 ttgctggcaa gaatatcgct ctcctatttg aaaaaacatc tactcgtact cgtgcagcct
     1621 ttacaactgc ggctatcgac cttggtgctc acccagaata cctcggagca aatgatattc
     1681 agttgggtaa aaaagaatct actgaagata ctgctaaagt attgggacgt atgtttgacg
     1741 ggattgaatt ccgcggattc agccaacgta tggttgaaga attggcagaa ttctcaggcg
     1801 ttccagtatg gaacggtcta actgacgaat ggcacccaac tcaaatgctc gctgactact
     1861 tgactgttca agaaaacttc ggtcgcttgg aaggcttgac attggtatac tgtggtgatg
     1921 gacgtaacaa cgttgccaac agcttgctcg taacaggtgc tatccttggt gtcaatgttc
     1981 acatcttctc accaaaagaa ctcttcccag aaaaagaaat cgttgaattg gcagaaggat
     2041 ttgctaaaga aagtggcgca catgttctca tcactgaaga tgctgatgaa gcagttaaag
     2101 atgcagatgt tctttacaca gacgtttggg tatcaatggg tgaagaagac aaattcgcag
     2161 aacgtgtagc tcttcttaaa ccttaccaag tcaatatgga cttagttaaa aaagcaggca
     2221 atgaaaactt gatcttccta cactgcttgc cagcattcca cgatactcac actgtttatg
     2281 gtaaagacgt tgctgaaaaa tttggtgtag aagaaatgga agtaacagac gaagtcttcc
     2341 gcagcaagta cgctcgccac ttcgatcaag cagaaaaccg tatgcacact atcaaagctg
     2401 ttatggctgc gacacttggt aacctttata ttcctaaagt ataattttag ataataaacc
     2461 gtctaccaac agctatgagg gctgcgacta atagctttag tccggtcctc ttttatgtaa
     2521 tggtaatcta ttatttctta taaaatatgt gaaaaatcat taaattgaaa tctaaacgca
     2581 ttctattgag tgtgataaag gagaatttat ggcaaatcgt aaaattgtag tagctttggg
     2641 aggaaatgcg attctttctt ctgacccatc agcaaaggct caacaagaag ctttagttga
     2701 aacagctaag catcttgtaa aattgattaa aaatggagat gatctgatta tcactcacgg
     2761 taatggacct caagttggga atctcttgct ccaacatttg gcatcagact ctgaaaagaa
     2821 ccctgccttc ccactcgact cacttgtcgc tatgacagaa ggtagcatcg gtttctggtt
     2881 gaaaaatgct ttgcaaaatg ctctcttgga tgaaggcatc gaaaaaaatg ttgcctctgt
     2941 tgtaacgcaa gttgtcgtag ataaaaatga tccagctttt gttaacttga gtaaaccaat
     3001 cggtcctttc tattcagaag aagaagcaaa agcagaagcc gaaaaaagcg gagcgacttt
     3061 caaggaagat gctggccgtg gctggcgtaa ggtcgttgcc tcaccaaaac ctgttgacat
     3121 caaagaaatt gaaaccatcc gtactctttt aaataatggt caagtcgtcg tagctgcagg
     3181 tggtggcggt attcccgtcg tcaaagaaaa caatggacat ttgactggtg tcgaagcggt
     3241 tattgataaa gacttcgctt cccaacgttt ggcagaattg gttgatgcag acctcttcat
     3301 cgttttgaca ggtgtagatt atgtatttgt taactacaac aagccaaacc aggaaaaatt
     3361 ggaacatgtg aatgttgccc agctggaaga atatatcaaa caagatcagt ttgcaccagg
     3421 tagcatgctt ccaaaagtag aagcagctat cgcttttgtc aatggtcgtc cagaaggaaa
     3481 agcagttatt acttcccttg aaaatctagg cgccttgatt gaatctgaaa gcggaacaat
     3541 tattgaaaaa ggataagttg ttttactaat aagatgtatt ctatttctag tatctttata
     3601 tcaaattaga aattattc
//
LOCUS       DAVHIU010000139         3607 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVHIU010000139 DAVHIU010000000
VERSION     DAVHIU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.141"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            54..281
                     /locus_tag="VVN06_001883"
     CDS             54..281
                     /locus_tag="VVN06_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563372.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            569..3466
                     /gene="nanA"
                     /locus_tag="VVN06_001884"
     CDS             569..3466
                     /gene="nanA"
                     /locus_tag="VVN06_001884"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU5563373.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKAFD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1224 a          614 c          823 g          946 t
ORIGIN      
        1 atgcgaaata acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat
       61 ttaaggcatt caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt
      121 ttttcgacag ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc
      181 tggataagat tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg
      241 cttatgaaaa gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtataaa
      301 tagcataagc tgatgtccat catttgctta taaagagata ttttagttta attgcagcgg
      361 tgtcctggta gataaactag attggcagga gtctgattgg agaaaggaga ggggaaaatt
      421 ggcaccaatt tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa
      481 agaaagttaa taaaagacaa actaagtgca ttttctggag taaatgtctt atttcagaaa
      541 tcgggatata gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg
      601 ttatagcatt aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt
      661 tggaacgtct cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac
      721 tcaactttcg ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga
      781 gactgaactt tctggcaata agcaagaaca agaaaggaaa gataagcaag aagaaaaaat
      841 tccaagagat tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga
      901 tgttgaaacc aatgcttcaa atggtcagag agttgattta tcaagtgaac tagataaact
      961 aaagaaactt gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggctccagc
     1021 attctataat ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc
     1081 agtttacaat aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacgg
     1141 taattacaac gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac
     1201 agttgaaaaa ccgacaccag aactacctaa aggccgagtg cgcctctacg taaacggggt
     1261 attatctcga acaagtctga aatctggcaa tttcattaaa gatatgccag atgtaacgca
     1321 tgtacaaatc ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat
     1381 tcggaatctc actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca
     1441 actttttaaa cgctcagatt tagaaaaaaa actacctgaa ggagcggttt taacagagaa
     1501 aacggacata ttcgaaagcg ggcgtaacgg taaaccaaat aaagatggaa tcaagagtta
     1561 tcgtattcca gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg
     1621 ccgtctccat tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa
     1681 tggtaaaact tggggtgacc gagtaaccat taccaactta cgtgacaatc caaaagcttt
     1741 tgacccatcg atcggttcac cagtgaatat cgatatggtg ttggttcaag atcctgaaac
     1801 caaacgaatc ttttctatct atgacatgtt cccagaaggg aagggaatct ttggaatgtc
     1861 ttcacaaaaa gaagaagcct acaaaaaaat cgatggaaaa acctatcaaa tcctctatcg
     1921 tgaaggagaa aagggagctt ataccattcg agaaaatggt actgtctata caccagatgg
     1981 taaggcgaca gactatcgcg ttgttgtaga tcctgttaaa ccagcctata gcgacaaggg
     2041 tgatctatac aagggtaacc aattactagg caatatctac ttcacaacaa acaaaacttc
     2101 tccatttaga attgccaagg atagctatct atggatgtcc tacagtgatg acgacgggaa
     2161 gacatggtca gcgcctcaag atattactcc gatggtcaaa gccgattgga tgaaattctt
     2221 gggtgtaggt cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt
     2281 gataccggtt tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt
     2341 catctattca gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg
     2401 tcaggtagac ggtcaaaaga tccactcttc tacgatgaac aataaacgtg cgcaaaatac
     2461 agaatcaacg gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac
     2521 tggagatctt caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa
     2581 acgttatcca caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcataa
     2641 tggaaaagaa tacattatcc tcagcaatgc aggtggaccg aatcgtgaaa atgggatggt
     2701 ccacttggca cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca
     2761 aaaaggagag tttgcctata attcgctcca agaattagga aatggggagt atggcatctt
     2821 gtatgaacat actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg
     2881 ggaatttttg agcaaagatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga
     2941 gatgggcaaa ggagagatgg gcaaaggagt tattggcttg gagtttgact cagaagtatt
     3001 ggtcaacaag gctccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca
     3061 gtatgatagc aagaccttgt tgtttgcagt agataaggaa gatatcggac aggaaattat
     3121 tggtatagct aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc
     3181 cagagttcct ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac
     3241 agggggagtt aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga
     3301 ttcgcttgta actcttacta caaaagaaga ttatacttac aaagctcctc ttgctcagca
     3361 ggcacttcct gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc
     3421 tttcttcctt ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa
     3481 acatttgatt ttgtaaaaat agaaggagat agcaggtttt caagcctgct atcttttttt
     3541 gatgacattc aggctgatac gaaatcataa gaggtctgaa actactttca gagtagtctg
     3601 ttctata
//
LOCUS       DAVHIU010000140         3604 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVHIU010000140 DAVHIU010000000
VERSION     DAVHIU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.142"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..643
                     /gene="dnaE"
                     /locus_tag="VVN06_001885"
     CDS             <1..643
                     /gene="dnaE"
                     /locus_tag="VVN06_001885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5563374.1"
                     /translation="FVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLGIGVSKHPL
                     QAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADDSKKKLDVT
                     LFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQVKNHESDQ
                     EISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKTIYR"
     gene            726..1733
                     /gene="pfkA"
                     /locus_tag="VVN06_001886"
     CDS             726..1733
                     /gene="pfkA"
                     /locus_tag="VVN06_001886"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU5563375.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAALVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            1792..3297
                     /gene="pyk"
                     /locus_tag="VVN06_001887"
     CDS             1792..3297
                     /gene="pyk"
                     /locus_tag="VVN06_001887"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5563376.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         1157 a          623 c          807 g         1017 t
ORIGIN      
        1 atttgtgaaa gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga
       61 agattggacg gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag
      121 caaacatcca ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa
      181 tttgtcagaa aatagctatg ctattatctt ggttgaagtt cagaaaataa aagtgattcg
      241 taccaaaaag ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga
      301 tgtcactctc ttttcagact tatatcgtca ggttggacag gaaataaaag agggagcctt
      361 ctactatgta aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga ttgcacaaga
      421 aataagagaa gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca
      481 agaaatttca cgcattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga
      541 agaggaacag aaaaccatcg tttctcccca tcattttgta gctaaatcca atgaattaga
      601 ggagaaattg aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa
      661 gaattttaaa atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa
      721 ccaatatgaa acgtattgct gttttgacta gtggcggaga cgcacctggt atgaacgctg
      781 ccatccgtgc agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg
      841 acggatatgc tggtatggtt gccggtgaaa ttcatcccct agatgcagct ttagtagggg
      901 acatcatttc tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac
      961 ttgaagggca acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg
     1021 ttatcggtgg tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag
     1081 ctattggtct tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt
     1141 ttgacacagc ggttactact gccatggacg ctatcgataa gattcgtgat acatcatcaa
     1201 gtcaccgtcg tacttttgta atcgaagtta tgggacgtaa cgctggtgat atcgctcttt
     1261 gggctggtat tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg
     1321 aagatatcgt agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg
     1381 tcttagctga aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag
     1441 atacaagcga ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg
     1501 cgcgtgaccg tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag
     1561 gtatcggtgg tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg
     1621 gtactgcaga agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca
     1681 acccgcacaa agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa
     1741 tttagttaat aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa
     1801 cgtgtaaaga tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc
     1861 ggtgaggatg gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct
     1921 aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa
     1981 gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt
     2041 ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct
     2101 aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa
     2161 tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt
     2221 gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa
     2281 gatgatgcaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa
     2341 aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac
     2401 gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt
     2461 actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt
     2521 caattgttcg ctaaaatcga aaaccaacaa ggtatcgata acttagacga aatcatcgaa
     2581 gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg
     2641 gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc
     2701 actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta
     2761 tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgctgtc aggcgagtct
     2821 gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct
     2881 caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag
     2941 acagaagtaa tggcttctgc tgttaaagat gctactaact caatggatat caaattggtt
     3001 gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct
     3061 gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt
     3121 gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa
     3181 cgtaaagcga tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt
     3241 gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa
     3301 aaatataaaa acctatcata tccagcttta gagcttgtgt gataggtttt ttgtatagag
     3361 ggtaacaaag aggcaaaact ttcataatcg attgaaataa gatatgcaca aattgattag
     3421 aaagtcaaat gaatttctac aaatgtttta gcaatcgtaa tgtacttgtc tagattcgat
     3481 ctgatatatt ttcgatttaa tgatatggta tttaaaacct ccaaagtagc ttgctccatt
     3541 cttttactta cgtgagtgta gatgttattt actgttttag cgtttttgtg ttccactcta
     3601 acca
//
LOCUS       DAVHIU010000141         3573 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVHIU010000141 DAVHIU010000000
VERSION     DAVHIU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.143"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(103..1281)
                     /locus_tag="VVN06_001888"
     CDS             complement(103..1281)
                     /locus_tag="VVN06_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5563377.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(1737..3014)
                     /gene="ltrA"
                     /locus_tag="VVN06_001889"
                     /pseudo
     CDS             complement(1737..3014)
                     /gene="ltrA"
                     /locus_tag="VVN06_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1139 a          743 c          577 g         1114 t
ORIGIN      
        1 caatgaagta aatcttccca taataaaaca cacaatatca agttttttca acacctgata
       61 ctatgcgctt ttctgatttt taaagacttt ttaaccactc tctcatttaa aataatctcg
      121 tctgatataa attaaaatag cttctatcat cagacaaatg gctgatagcc aaaaactgat
      181 gctaatacca aaactctcag taatatagct cattagcaaa acaaatactg aaaatgctaa
      241 tgtagaaatc acttcaagaa cggaatagac attaactaaa tgattttcct ctactgtttc
      301 ctgaagaaat acactttcag gaacttcttt tagttgcgat aacataccaa ctaaagctga
      361 aaataataaa aacatctgtg cgtttggaaa atatagaata gtcagtgtca ctattgccat
      421 agctacaaga ggaaaaagaa tactttccca tttagcagca aggaactttt cagatagcct
      481 aaaagcaatt aagccactaa ttataatacc aatagagtat gctgtattag aatatcccca
      541 gtaactttcc gttttattta ataactccgt tacaaaaaca agtataatgg aagaaaccca
      601 aatcgtattt gaaaaaattt ccaataaatt tgctgataca aaaagtctta atctaggatt
      661 tctagcaact aacttccaac ctttgagcaa aatttcaaga ttagtttctg actctaacac
      721 ctccacttca gcgttaggaa gaaataacat cagaaagcta gaaatgatat acaagactaa
      781 attgatacac gtggtaggta acagaccaat tgttgcaaac aagagtccac ctaatcccca
      841 acctatcaat tgaacagctt caccagtcat tgataaggct gaattagcct tacccaaatc
      901 ggtcgcatag cgtggcacaa tagcatagga aacgggtgct gcaaaaccat ctagtatgga
      961 aattgcaaca acaaatagat aggtcaccaa aggcgctacg gattgcatta cggtaaacat
     1021 tcctaccagt atcgccaata atatagtctt tccaaattga gataaagata aaaccctatt
     1081 tagcgctaac cttttagtaa ccaacggaac taaaagactc gcaacaaaag aggatattcc
     1141 tattaagata ggaactagtg atgtggcaat tactgatttt gaaataatgt atatgttagc
     1201 aatgattgtt actctaaaga aaatatctgc taaatttgca aataattgag agactaacaa
     1261 cttgacaaat gaattagaca tgtagcgctc cccctttagt tacaaacttt ggaattaaat
     1321 ttgattttta atattttagc ataaaatagc agaaaacaaa agtgaggttt tttacatgaa
     1381 aaaacaaaaa ctattgcctt tattattcct aattttagga ggaattatga ttatcgttgt
     1441 tggctagtac taagaagtta gttagttagt agaataatta catcttcaat cgaaaaagag
     1501 actggaaaaa gtcttaaaaa tcagaaaaac gctcaaatcc tttggcgctc taagcttttc
     1561 taatgataga tttagatagt caaaaaaata tctgaaaagt tcccaggatc gtcgtctcag
     1621 catcaaaaat acgtgaaatt tgcataatta aaataaattt caggggagtt aatcgagtag
     1681 gggataatct ctagcccctc tcacaccacc gtacgtgccg ttcggcatac ggcggttcaa
     1741 ctaactttta acgcatgtcg ttcaaggtaa taatccaaac acgaaaccag tccacgtttt
     1801 tccaggactg gttttgatat agcacgttta agtaccgact tctgagctac taattgataa
     1861 tggtcgcccc agccagatac cttatctgct atccatttag gaactcctaa cttaagcaat
     1921 ccccataatc gtctcgattt cttcttccat tgcttccaga taatcactcg taggcgagta
     1981 cgcaagcgct catctatgct ggcgactata cttttcatat ttcccaatga gaaatagttt
     2041 atccatcctc gaatagacaa attcagttgc tcaatacgtc ttgttaggtc tatactccat
     2101 ttcctctgtg ttagtttctt caatttaagc ttaaatctcc gaacactatc ttgatgtgga
     2161 cgacttttcc aaccatctga tgatttccag aacccaaaac ctagatattt caactctctt
     2221 ggtctggtaa tcttagtctt ggtcatgttt actttcaaac ctagccgttt ttcaataaaa
     2281 cgactgactg aatacatcat acgcttagcg gctgcctcgc ttacgaccgt aatcacacaa
     2341 tcatctgcgt agcgcacaaa tcgaagtccc ctcttttcta attccttgtc caattcatta
     2401 agcatgatat tggataagag aggagataaa tttcctccct gtggtgtacc aactagtgtt
     2461 ttataacgtt gaccattaat gataacacct gaatgaagat acttacgaat caaggattcc
     2521 gtatctccgt cttcgataat gttatgtact aaggacatca atctatcttg aggaactgta
     2581 tcgaaaaatt tctctaggtc tatatccact atccactcat agccgtcatt taagtattct
     2641 aagagcttca tgatggcttt ttcacatgac ctatttggtc tgaaaccata actcgtatct
     2701 gagaaatggg gttcacaaat ggggctcatg acttagacaa tggcctgttg aatcattcta
     2761 tccataactg ttggaattcc tagttgacgg atacctccgt ctggtttagg tatctcaact
     2821 ctaagaactg gttgaggctt atattttctc tgttttatca gttccttagt caagcgccag
     2881 ttttgtctga gataattatc catctcttcg atagtcattc catcaatccc agctgagcct
     2941 ttattggatt ttacttgatt gtaggcttcc agcatatttt cgcgtgataa tatcttatct
     3001 agcaattttg acatgtgcgt attcctttct tgttttggtg cctgagggac atcttatatt
     3061 ggtgagcctt catctagtca atacgtgaca gactccagtt ctatcagacc tctcgtttta
     3121 cagacacaag tgaagtagga ttacttcaat ctgtcgtttt ctctttctag cgacagacct
     3181 caaacagtct ctccccagac tgtttgagta ttcacccctt cgctccactt ccattacgga
     3241 agtttcctca ctactatggg ttcggctgac ttctcatgat tcgttgttac tacgctttca
     3301 gcactcatga gacctcacgg gataagtcgt acatctttcc tcgtttatac aagatatgag
     3361 gacgttaaaa ggttacagtg aaaataggaa atctgacgca gagtctttag actctagaaa
     3421 gatttatctt ttcacacaaa ccttagtccg aattcaatta ctctcgtgat ttacgcatat
     3481 aggttacgac tacctttttg gactttagag tttgaagcct ccttatccgc tatttacgcc
     3541 ttactatcac gttcctgttc gtagagccac gat
//
LOCUS       DAVHIU010000142         3566 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVHIU010000142 DAVHIU010000000
VERSION     DAVHIU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.144"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..159
                     /locus_tag="VVN06_001890"
     CDS             <1..159
                     /locus_tag="VVN06_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HEU5563378.1"
                     /translation="IILSAKIDDDTAIAHDGESWGKLVSIGFDIIQTDWPLLLYQYLV
                     EKNKKIKK"
     gene            186..1247
                     /locus_tag="VVN06_001891"
     CDS             186..1247
                     /locus_tag="VVN06_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5563379.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1264..2268
                     /locus_tag="VVN06_001892"
     CDS             1264..2268
                     /locus_tag="VVN06_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563380.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSATQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIKEKVTMYINPRDIMQF
                     "
     gene            2280..>3566
                     /locus_tag="VVN06_001893"
     CDS             2280..>3566
                     /locus_tag="VVN06_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5563381.1"
                     /translation="MRKSLNWKDCLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGT"
BASE COUNT         1231 a          500 c          654 g         1181 t
ORIGIN      
        1 attattcttt cggctaagat tgatgatgat acagctattg ctcatgatgg cgaatcttgg
       61 gggaaattgg tttcaatagg atttgatatt attcaaacag attggccatt attactttat
      121 caatatctag ttgaaaaaaa taaaaaaata aaaaaataaa aaaataaaaa taaaggagaa
      181 ctatcatgtt caaaaacttt aaaaagattg cattcacttc ttcactaatt ttacttgctg
      241 cttgctcaaa caatgcttca aatacaaatc agtctactga tgaaggaagt gttgataaat
      301 ctcaagaaat tgtaatctat acaaattcgg cttctaatgg acgtgcagat tggttacaaa
      361 ctaaagcatc agagcaagga tacaatcttc gtttggtaga tatttcaggt ggagaactag
      421 ctgaccgttt gattgcagaa aagaataatg ctgtggctga tatggtaatt ggattaaata
      481 aattagaatt taatcgtatt aaagctgaaa aacttcttgt aaaatactca ccaaaatggg
      541 ctgatgaagt agatagctct ttgggagatt ctgaagggta ttattcacca attgtaaatc
      601 aaccattagt tttgattggt aataaagatg ctaaaatgcc atccgactgg acagatttta
      661 ctgattcatc ttataaaggt aaatatggta tttcaaaatt aagtacaggt acatctaaga
      721 acatctttgc aagtattgtt tcgagatata aagatgaaaa aggtgagtta ggaatatctg
      781 atgaaggttg gaaagttgca aaagcttacc ttaaaaatgc acacgtttat gcagaaggtg
      841 aagactatat ctcttcaatt atggataata ataatgatct aaaatatagt atgatgtggg
      901 gatctggtgt tctacaaaat caaaaagaac gtgaatataa attccaagta atgtcaccaa
      961 aagttggtgt tccttatgta actgaacaag ttgggatttt aaatacaagt aagaagcaag
     1021 cattattaaa agaatttgta gattggtttg gctcttcaga acttcaaaaa gaatggtctg
     1081 ataaatttgg ttcaattcca gctaataaaa aagctctaga gcaagcaaaa gatgaactca
     1141 aacagtttgc taatagtgtt aaaccacaag aacttgactg ggagtttatc gggaaaaaca
     1201 tcgattcttg gattgaaaaa gcacagcttg aatttgttga acaataatag ttaggattaa
     1261 gtgatgatta catttaagga tattcaaatt aagtataatg attttattgc catcgataat
     1321 ctcaatttaa caatcaatga gggagagttt ttcacatttt taggaccaag tggttgtggg
     1381 aaatcaacaa ctcttcgtgc actagttgga tttttggagc cttcacaggg gcaaatacta
     1441 gttgataatc aagatattac gaatttagat ccggaaaaac gcgaaattgg tattgtgttt
     1501 caaagttatg cattattccc aacaatgact gtttttgata atattgcttt tggtctaaaa
     1561 gttaaaaaag tttctcctga aattattaaa gctaaagttc atgaaattgc gaaaaaaatt
     1621 aatatctcag acgagcaact aaaccgaaat atttctgaat tatcaggggg acaacaacag
     1681 cgtgtagctt tagcacgtgc attagtatta gaaccaaaaa ttctttgttt ggatgaaccg
     1741 ttatcaaacc tagatgccaa actacgagtt tcgttgagaa aagaattgaa acgattgcaa
     1801 aaagaactag gaatcactac tctttatgtc acacatgatc aagaagaagc attaacatta
     1861 tcagatagga ttgctgtgtt taataatgga tttattgagc aagttggcac gccgtatgat
     1921 atttataatc aatccgcaac tcaatttgtc tgtgatttta ttggctcaat caataaaatt
     1981 agccaagaag ttcttaaaaa attacaaggc gagaataaat actttaaaga aactgaagga
     2041 tacatacgct tagagcgtgt taagttagag aaaacttcac ctcaagattt atctataaca
     2101 ggggtaatta gtgatattga gtttaatggt gtacttatta agtataaagt agacttgtcg
     2161 aatagtgaaa gtattcatat tatcgaaaag aatgatggac tatcacgtca tataatcaaa
     2221 gaaaaagtca ctatgtacat taatccgcgt gatataatgc aattttaagg agattgtgca
     2281 tgagaaagag tctaaactgg aaagattgtc tcataagatt aggagtttta tggctaatct
     2341 ttgccctgat tatttatcca aatattgaac ttttgtctgg agtgttttat aaaaatggtt
     2401 catttactct agaggcattc aataagatta tacactctca gagagcgatg agtagtatat
     2461 ttaatagctt tattttggcg atttcaatgg tctttactgt aaatattgtt gggacattga
     2521 ttgttctact cacagagtac tgggatatta ggggttcaaa aatacttaaa ttaggttata
     2581 tgagctctct agtatatgga ggaattgtat tagcgacagg atataaattt gtttatggcg
     2641 ctaatggtct tgtaactaaa acgctctcaa gtataattcc aggtcttgcc cctaactggt
     2701 ttcaaggatt tggtgcagta ttgtttatta tgacatttgc ttgtacctca aatcacataa
     2761 tgtttttaac aaatgcagtt cgtagtgttg attatcatac aattgaggca gctaaaaata
     2821 tgggtgcaag tcctttaaaa gttttcttta gtgtggtttt tccaactttt aaacctacgt
     2881 tatttgcctt aaccatctta acttttttga caggtctttc agcagtttct gctcctttga
     2941 tagtaggggg gaaagatttt caaactatta acccattgat tatcagtttc gctaaaacta
     3001 caagttcgag agatttagca gctttactag ctatcatgtt aggtttagca actattctat
     3061 tattgacact tttgaatcat gtcgaaaaag gaggtaatta tgtatctgtt tcgaaaacca
     3121 aggctagtat taagaagcag ataatttctt ctccattatg gaatactctt gctcacattg
     3181 ttgcgtatgt attgtttgca atctatatgt taccaatagt tttgataatt ctatactcgt
     3241 ttacagactc gttgacgatc aagacgggaa ctttagatct atctaaattt acgttagcga
     3301 actatgctaa tttatttaca gatgctcaat catttcgtcc ttacctagtc agcgttgttt
     3361 atgcaatttt agctgcggta attgctacaa ttatagcaat agtaatttca cgtattgtac
     3421 ataaaaacaa gagtaagttc gataaattct ttgagtatgg tgcactgata ccatggatat
     3481 tgccaggtac attgattgca ctaggattaa tgttcactta taatatacct catttaatac
     3541 tatttaattt agttttagta ggaaca
//
LOCUS       DAVHIU010000143         3562 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVHIU010000143 DAVHIU010000000
VERSION     DAVHIU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.145"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1869)
                     /locus_tag="VVN06_001894"
     CDS             complement(<1..1869)
                     /locus_tag="VVN06_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU5563382.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFD"
     gene            complement(1931..3553)
                     /locus_tag="VVN06_001895"
     CDS             complement(1931..3553)
                     /locus_tag="VVN06_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5563383.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGSGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1208 a          614 c          652 g         1088 t
ORIGIN      
        1 atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca aggtatgttc
       61 attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa gagagtattg
      121 attttcatat agggtaagat tatctttttg atagatatct ttaaaaccat acttatcaat
      181 aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa tactattatt
      241 tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat ctaaagtaga
      301 gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt tgaatttcat
      361 actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat ttccaatata
      421 tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag aagcaaatcc
      481 ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt caaccagtat
      541 aaataaagag attagaatgg caaatagatt cacagatata aactttttga taactgcaag
      601 gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca aatctgttaa
      661 aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta caagaaaaag
      721 cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt ctgctgctgt
      781 gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt ttggagtatg
      841 catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta gaaatgcaaa
      901 gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa aaaataaaat
      961 ggtcaaaata aagggaagta gtccaacaaa aatcattggg atggccccat actttgttgt
     1021 gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac tttcagtttg
     1081 aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata gagtgggaag
     1141 agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat caagaacaga
     1201 tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa taagaaacaa
     1261 tactgtcata tatccaaaat aataattttg aataaataag attgacagac ttgtaaagta
     1321 caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta aaggaatcaa
     1381 gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac tcatcagagc
     1441 ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca atttattcaa
     1501 actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca gatagacagc
     1561 atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac tacccaagta
     1621 ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt aaaacagact
     1681 actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt gatgaaagcc
     1741 atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata gatatactcc
     1801 agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg tttttaaaaa
     1861 tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca actgagtttt
     1921 gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct gcattttcta
     1981 ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat aagacaatga
     2041 tatggttagc caaagtttga ataaactcgt ggtcatggct ggcaaagatg attgattctt
     2101 taaagttttt caatccatca ttcaagcttg agatagattc caagtccaag tgatttgttg
     2161 gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc atgacacgaa
     2221 ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca gagaagagca
     2281 tacggccgag gaagccacgt aggaaagtat tgtcatcttc ttctttactt gcgaattgac
     2341 gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct tttggtaggt
     2401 aagattgact agttgtaact ccccacttga cagttccttc atagtcaata tctcccatga
     2461 ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct gtcttatcat
     2521 ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc tttacagtta
     2581 gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag ttgataaatg
     2641 gatatttacg actagatggc acaatctctt ctagctcaat cttatcaagc attttcttac
     2701 gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaaca aattcttgca
     2761 attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta gcagcaagct
     2821 cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt ccaaagtcaa
     2881 ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat actacgataa
     2941 ctgtgttatc aaagtcaatt aagaagtctt ctaaccaagt aatcgattgg atatccaaac
     3001 cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct ttggcgagga
     3061 gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt aattcttctg
     3121 gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa cctccaagtt
     3181 cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag aaatcttcct
     3241 tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt cccatgataa
     3301 cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga acagagagac
     3361 gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca gctaaaattt
     3421 ttaaaaaggt tgattttccg gaaccattag caccgattaa tccgtaagta tttccttctg
     3481 taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt gaaacatcag
     3541 atactgtaag caatattttt ct
//
LOCUS       DAVHIU010000144         3482 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVHIU010000144 DAVHIU010000000
VERSION     DAVHIU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.146"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..129
                     /locus_tag="VVN06_001896"
     CDS             <1..129
                     /locus_tag="VVN06_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5563384.1"
                     /translation="SKTMQKILDLRKKYHLQMQVERYIEDDSGMPESDKSFIDTGN"
     gene            319..1755
                     /locus_tag="VVN06_001897"
     CDS             319..1755
                     /locus_tag="VVN06_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU5563385.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDFHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMIHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            1807..2046
                     /locus_tag="VVN06_001898"
     CDS             1807..2046
                     /locus_tag="VVN06_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU5563386.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2176..2490
                     /locus_tag="VVN06_001899"
     CDS             2176..2490
                     /locus_tag="VVN06_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU5563387.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2608..>3482
                     /locus_tag="VVN06_001900"
     CDS             2608..>3482
                     /locus_tag="VVN06_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU5563388.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQC"
BASE COUNT         1175 a          441 c          712 g         1154 t
ORIGIN      
        1 tcaaagacaa tgcagaaaat cttagattta agaaaaaaat atcacttgca gatgcaagtt
       61 gaacgttata tagaagatga ttctgggatg ccagaaagcg ataagtcgtt tatagacaca
      121 gggaattaga attattgtga gagtttttgt agtttgaaag tattgaaaat cgtaaagata
      181 tcattacgag aatacaagaa gatattgttc tatttccatt aaaaattact tctttcctta
      241 acaataagga aaaagaatgc gttttcatct tttggtttga ggtttataat aagagtatca
      301 aagtcaggag gaaaaaagat gactactttt aaagatggat ttttatgggg tggtgctgtt
      361 gctgctcatc aacttgaagg tggatggcaa gaaggtggca agggaattag tgttgctgat
      421 gttatgactg ctggtcgtca tggagtagct cgtgaaatta cttcgggagt tttagagggt
      481 aaatattatc caaatcatga ggcgatagat ttttatcatc gttataaaga agacatagca
      541 ctttttgctg aaatgggatt caagtgcttc cgtacctctc ttgcatggac acgtatcttt
      601 ccaaaaggtg atgagttaga gccgaatgaa gaaggattac agttttatga taatcttttt
      661 gatgaatgct taaagaatgg tattgaacct gtcatcactc tatctcattt tgaaatgcct
      721 tatcacttag tgaccgaata tggtggttgg aaaaatagga aattgattga tttctttgct
      781 cgttttgcag aagtcgtatt taaacgttac aaagataagg ttaaatattg gatgactttc
      841 aatgaaatca ataatcaagc gaattatcag gaagattttg caccatttac taactcaggt
      901 attgtatatg aggaaggtga taatagagaa gcaattatgt atcaagcagc acattacgaa
      961 ttagttgctt ctgcacgagc tgtaaaaatt ggtcatgaga ttaatccaga ttttcaaata
     1021 ggttgtatga ttgcgatgtg tccaatttat ccagctactt gcaatcctaa ggatatctta
     1081 atggcaatga aagctatgca gaagcgttat tattttgctg atgtgcatgt tttaggtaaa
     1141 tatcctgagc atattttcaa gtattgggaa cgaaaaggta tttcagttga ttttactgcc
     1201 caggataaag aagatttact tggtgggact gtagattaca ttggtttcag ttactatatg
     1261 tcctttgcta tcgactttca tcgtgaaaat aatccttatt ttgattatct tgaaacagaa
     1321 gatttagtga aaaataatta tgttaaggct tctgaatggg agtggcaaat tgatccagaa
     1381 ggtttacgtt atgcgttaaa ttggtttaca gaccactatc acttaccact ctttattgtt
     1441 gaaaatggtt ttggagctat agatcaagtt gcagcagatg gtatgataca tgatgattat
     1501 agaattgaat atctaggtgc ccatattcgt gaaatgaaaa aggctgtagt tgaagatggt
     1561 gttgatttaa tgggttatac tccatgggga tgtattgatt tggtttcagc tggtaccggt
     1621 gaaatgcgga aacgttatgg ctttatttat gtagataaag atgataatgg gaagggaagt
     1681 tataatcgtt ccccgaaaaa atcttttggc tggtataagg aagttatttc atctaacggt
     1741 gaatcagtag aatagtataa acaaactgag attattctca gtttgtttag catattgagg
     1801 tgacatatga ttggaagtat tgcagcaatt ttaacaacat ttgcattttt accacaagtt
     1861 tttcgtgtag ttaagacaaa ggacacagga tctattgctc taggtatgta tgttatgcaa
     1921 gttattggta ttgcattgtg gcttgctcat ggtattcgta taggagactt gcctttgatt
     1981 ttagcaaata gtgtttcatt tctactctca ggaataattt tattttataa attgaagtat
     2041 aaataatttt tttccatatc atttaggaaa atgagggaat tatcattgga aacgctttat
     2101 tttataataa aaatagataa caaataagcc aacttagcac agctttgatt ggatttaaga
     2161 aaaggagaaa atattatggc aaaagcttta attatttgtg cagcaggaat gtcttcatca
     2221 cttatggcac agaaaacaac agagtttcta aaaggaaaag ggaaagatat tgtagttgat
     2281 gctattagtg ctactgaagg agggaaagct atcgcagcag cagcttttga tttgtatctt
     2341 gtcagccctc aaacaaaaat gtactttaag caatttgaag aggctggttc taaagcagga
     2401 aaaccagttg tacaaatacc tcctcaagca tatattccta ttccaatggg aattgaaaaa
     2461 atggctaatt tgattcttga gaatatttga tgaacacatg aaatgtcaat aaatatttag
     2521 gttaagataa attgttttga gtttgatata aattgattta agtgtataat atgcttcaat
     2581 atatgaaaac atacgtttta ggagggaatg aatgtgttaa acattaaaga gaaagatatc
     2641 cttcaatttt taataaaaaa taaggaacgt tttgttacaa gcaaagaact ggcagaatat
     2701 ttatcctgtt cagatagaac tgtccgtaat gtactgaaat taattgagaa gacaatgatt
     2761 attcaaggag tccggttgat ttcaaaacaa ggtcaagggt atcaaatatt ttttgagaat
     2821 caaggcgctt accaggagtt tagacaaact tatgaactgg aagaagacta tacgaaaact
     2881 gcggtttcaa aaggtgatga tcgtttagta tttatcttaa ataaattatt atttgagcaa
     2941 gttccagttc tttttgatga tttagctgat gaactttacg ttagtcgttc aacattatct
     3001 catgatttca gaaagatacg agtgatgtta tcagagtata atttgtccat tgagagtagg
     3061 gcgaataaag gagtttatgt ctcaggcgag gaacgtgata aacgacgttt tatcataaat
     3121 tatttcctag agaatcaatt ttttaaaaca atacattgct atgtaaaatt taactttttt
     3181 gatcaaactg ttccattaga agaatttgct agaattgttt tggatgaatg ccaagaggcc
     3241 aacctaaaac tatctgattt tgtactacag aatttagtag ttcacatagc attgtctgtg
     3301 attcgtttaa aatccggatt tgaaataaaa aatatagact gtcagatgac tgatgatgca
     3361 atagagagaa aggttgccca aagaattctt tctaaagtaa gagaagtaac aaatcaggaa
     3421 tttccagttc aggaaataga ctacattact ttgcatttgt tagctaagag tcaacaatgt
     3481 ca
//
LOCUS       DAVHIU010000145         3396 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVHIU010000145 DAVHIU010000000
VERSION     DAVHIU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.147"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            42..779
                     /locus_tag="VVN06_001901"
     CDS             42..779
                     /locus_tag="VVN06_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU5563389.1"
                     /translation="MFILIINRPLNDSQSFKTKSNIAQALKHLDKPIIDLSGWQRPEE
                     INYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKTYKSHITELQKRNVPVAVYAY
                     VAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMNEGVENFRAKLASLGAKN
                     IGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKTDLDYDIHQYTSKGKIAG
                     FDHDLDINVISPLKNKEETFRKLFLKP"
     gene            874..1716
                     /locus_tag="VVN06_001902"
     CDS             874..1716
                     /locus_tag="VVN06_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU5563390.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            1716..2198
                     /locus_tag="VVN06_001903"
     CDS             1716..2198
                     /locus_tag="VVN06_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU5563391.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            2198..2818
                     /locus_tag="VVN06_001904"
     CDS             2198..2818
                     /locus_tag="VVN06_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5563392.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(3071..3166)
                     /locus_tag="VVN06_001905"
                     /pseudo
     CDS             complement(3071..3166)
                     /locus_tag="VVN06_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1036 a          696 c          617 g         1047 t
ORIGIN      
        1 aaattcatcc acttatttta tttagttttt ttgggattat gatgttcatt ttaatcatca
       61 atcgcccact taatgacagc caatcgttta aaacaaaatc caatatagca caggctttga
      121 aacacttaga caaaccgatt attgacctct ctggctggca gcgtcctgag gaaatcaact
      181 acgatgccct ctcacaaaat atttcaggtg ctattgttcg tgttcacagt ggtgctcaaa
      241 caacaaaaga aaatgatgct tcatttatca atggaataga caagacctat aagagtcata
      301 tcactgaact tcaaaagcgg aatgtcccag ttgctgtcta cgcttatgta gctggaaaaa
      361 gtgtccaaga aatggaaaag gctgccgaag ttttctacaa tgctgcctct ccttacagcc
      421 ctagttacta ttggcttgac gtggaagaca aaaccatgtc taatatgaac gaaggtgtcg
      481 aaaactttcg agcaaaacta gcatcactag gtgctaaaaa catcggcatc tacgttggtg
      541 tctacttcat ggaagaacac agtattgata caggcaagtt tacctctgtt tggattccgt
      601 cctacggctc agactcagga tttttggaga gcagtcctaa aactgattta gattatgata
      661 ttcaccaata cacatctaaa ggaaaaattg ccggctttga ccacgattta gatatcaacg
      721 ttatctctcc cttaaaaaat aaagaagaaa cctttcgaaa acttttttta aaaccttaaa
      781 agcatttgtt tatcatttgc tttttctttt tgtgtctgat tgtgatacaa tatagtaagg
      841 attttttgaa atagaaatag ttggaggaaa tatatgctct catggttagc acgcgttatt
      901 aaagggattg taattgctct tggatttatc ctaccgggaa tttccggagg ggttctagca
      961 gcaatcttag gaatctatga acgaatgatt ggctttctgg cccatccctt taaagacttt
     1021 aaagaaaatg ttttgtactt tattccagtt gccatcggta tgcttctggg aatcggctta
     1081 ttttcctacc cgattgaata cctgcttgaa aattatcagg tttttgtatt atggagcttt
     1141 gcgggagcta ttatcggtac agttcctagc ctcctcaaag aatcaactcg agaatctgac
     1201 cgagacaaga ttgatttagc ttggttatgg acaaccttta tcatttctgg attaggactc
     1261 tatgccttaa attttgtcgt tggaacctta agcgccagct ttcttaactt cgtcctagca
     1321 ggcgcactat tggcccttgg cgtcttggtt cctggcctca gcccatcaaa tttacttttg
     1381 attttgggac tatatgctcc tatgttgact ggttttaaaa cttttgattt cttgggaacc
     1441 ttctttccga ttggaattgg tgcaggtgca actctcatcg ttttttcaaa attgatggat
     1501 tatgccttaa acaactacca ctcacgcgtc tatcatttca tcatcggtat cgtcctatca
     1561 agtacccttt tgatcttaat tccaaatgca ggaaacgctg aaagtatcca atacacagga
     1621 ctttcacttg tcggttatgt catcatcgcc ttcttctttg cgctgggaat ctggcttggt
     1681 atttggatga gtcaattgga ggataaatat aaataatggc aaaaaaagtt aaaatcaaaa
     1741 aaacattggt ggaacaaatc ctatctaaag cagctatccc tcatcagggg attcaaatca
     1801 atgccctaga aggagagctt cctcaaggtt atgaacgaga tcagattttc aaaaccttgg
     1861 cgcttttggg agacaagacc ggaccgatta tcggaattgt ccctatcact cagcacttgt
     1921 ctgaaaagaa actagctaaa atttctggca ataaaaaagt gagcatgatt ccacaaaagg
     1981 acttagaaaa aacaactggt tacattcatg gagccaataa tcctgtcgga attcgtcaga
     2041 aacacaatta tcccattttt atcgataaaa ttgctctaga tttggatcga atgattgtct
     2101 ctgcaggcga agtcggacac agcattatcg tcgcgccaca agacttggct agctttgtaa
     2161 aggcagactt tgtagatatt ttggaggaca tcaagtaatg aaactctact ttgtccgcca
     2221 cggtcgtacc ctctggaacc aagaaggtcg ttttcaaggt gctagcggtg attctcctct
     2281 tcttcctgaa tccattgaaa ccctaaaacg acttggccag tatctcaagg aaattccttt
     2341 tgatcagatt tattcaagtg atttacctcg agcggtcaaa tctgctgaga ttatccaaag
     2401 tcaactccat accccctgtt ctttagaaat cgttcctaat ctccgtgaat ggcaacttgg
     2461 gaagttagaa ggtttgaaaa ttgcaaccct ggaagctatt tacccgcaac aaatccaggc
     2521 ttttcgttca aatcttgctc aatttgacac tcgaatgttc ggagccgaat ccctctatag
     2581 tacaactcag cggaccatcc aatttatcaa atcattaaaa gatagtccag ctgagcgtat
     2641 tctaattgtc ggacacggcg ccaatcttac tgccagtctt cgtactctcc taggctataa
     2701 agaaccactt cttcgtaaag atggcggtct agcaaatgcc agcctgacca ttatagaaac
     2761 ccatgatttt gaaacattca ctctcaatac ttggaatgat acttcttatc aatcataatt
     2821 atagcctagc cttctgaact agaaccatag gagatatagt cagttgaaac aaaaataaga
     2881 caaaagaacc ttgtaaaaag gtattgcaac ttgccaatac ctttttgata tgaacacgtg
     2941 tcttctcaac aggattgtag agcaaaatga aataagaact gaataatatg atttgagaat
     3001 ttaaatcaat ttataagaat gttttagaag cagttatgta ctattttaga ttcacttttc
     3061 tatactaaga tgagtaggga ggaaaaggta aaagtttatg cccaaactct tcacataaga
     3121 actctagctt acccattcta tggaatcttg cattatccat taaaacttcc agactcatac
     3181 ccttctaaaa tctattcaaa ccacgtcagc ttcacattat atatgttggt tacctcacaa
     3241 gttttatctg caacctcaaa gctgttcttt gagcagccta cagctagctt cctagtttaa
     3301 tctttgcttt tcactgagta tcaagttaaa aaaatgaggc tggtcaaaaa gtctttaaaa
     3361 ttataaaacg catcatatca ggtgtacatg aactac
//
LOCUS       DAVHIU010000146         3363 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVHIU010000146 DAVHIU010000000
VERSION     DAVHIU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.148"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(156..593)
                     /locus_tag="VVN06_001906"
     CDS             complement(156..593)
                     /locus_tag="VVN06_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5563393.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQISLEKVVADAESQMKD"
     gene            complement(669..1511)
                     /locus_tag="VVN06_001907"
     CDS             complement(669..1511)
                     /locus_tag="VVN06_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5563394.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHIGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            1623..2273
                     /locus_tag="VVN06_001908"
     CDS             1623..2273
                     /locus_tag="VVN06_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5563395.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            2254..2445
                     /locus_tag="VVN06_001909"
     CDS             2254..2445
                     /locus_tag="VVN06_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563396.1"
                     /translation="MSRKSTNFSSPLPTLKVLFSGFYFYHFILLSFGGRIFEKNGRVF
                     EKKKGSLTYEKITKNFCYI"
     gene            2471..3117
                     /locus_tag="VVN06_001910"
                     /pseudo
     CDS             2471..3117
                     /locus_tag="VVN06_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            3248..>3363
                     /locus_tag="VVN06_001911"
     CDS             3248..>3363
                     /locus_tag="VVN06_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEU5563397.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIPNHSYLSQLDVTYKT"
BASE COUNT          996 a          786 c          597 g          984 t
ORIGIN      
        1 ataaaaataa tactggattc cacaaacttc tattatcctt ccaaaatgac actataaagg
       61 ctaatacaat tcctataacg agatacattt cttactcctt taatagctac attttatcat
      121 aattatccaa agaaaaaaga gggcatttat ccctcttaat ccttcatctg actctctgca
      181 tcggccacga ctttttctag actgatttga ccaagttctg cctccatagt caactgaatt
      241 ctctccaatt tttgatccaa aacatcatga atatgagctc ctacagggca atttggattc
      301 ggattgtcat ggaaactgaa gagttgacct gtcttaccaa gacattcgac cgcctgataa
      361 acatctaaaa gactaatatc cttaaggtcc ttgacaatct ctgttccgcc cgttccacgc
      421 gctactgaaa tcagctctgc cttcttcaac tgggacaaga tctttctgat aatgacagga
      481 ttgaccccga cactagcagc cagaaaatca ctggtcacct tgctttcctt cccctcgagg
      541 gcaatgatta tcagcatatg agtcgcaatg gtaaatctac ttggaatttg catcctcttc
      601 tcctttttac gaggctaccc tgcctctact cttctttttc tattattata ccctttttag
      661 ttgtaatgtc aatcgttacc acttttcaac cagtcgtcta actcccgatc gcagccctct
      721 ttctgagcca attctctcaa aaattcctga tgatgagtat ggtggatccc attgaccaga
      781 ctttcatagt aaacctcaaa ataaggaagt ctcaggtctt tagccagctg caattcagct
      841 gccacatcgt agtctacccg tcggaagtcc atatctacca ggcccttgtc atcaaactcc
      901 aaaatcatat actgggcccg caagtccttc cgtagctgag cgtccaaaaa gaaaggttgg
      961 ccaatcgaac ccggattgac aatcaattgc ccaccagtcc cgtaacgaag caactgctgg
     1021 tgaatatgtc cataaacagc aatatcacag ggaggatgag tcaccaagcg gtcaaactcc
     1081 tcttgtttgc caatatgaat caactctcgc ccccagttct tatcaggcag atgatggcta
     1141 attcccaccg tcaaatcccc aaactgacga tgaatttgaa gaggttgatt gtggagcact
     1201 tcaatttctt ctagggaaat ttcctctaaa acatactggc actggcgcaa gagatagcgt
     1261 tgactggggc gagtactatc caattcctta cggacaccat gccaaagact gtcttcccag
     1321 tttcccaaaa ctctagccgt aatcggtagt tgatccaaca agtccaaaat ccttctacgc
     1381 cctgtccctg gcatgagaat atctcccaaa agccagtatt catccactcc tatctgccga
     1441 gcatctgcca aaacagcctc caaggcggtg gtatttccat gaatatctga aagaagagct
     1501 attttcgtca tatccatctc ctcgtttttt ctcttgcaat aagtataaca taaaaagtca
     1561 cagctagaga aatctagctt tttttgatat actagataaa gatattagac aagaggaaac
     1621 gaatgacccc aaacaaagaa gactatctaa aatgtattta tgaaattggc atagacctgc
     1681 ataagattac caacaaggaa attgcggctc gcatgcaagt ctctccccct gccgtaactg
     1741 aaatgatcaa acgaatgaaa agtgaaaatc tcatcctaaa ggacaaggaa tgtggctatc
     1801 tactgactga cctcggtctc aaactggtct ctgagctcta tcgtaagcac cgcttgattg
     1861 aagtttttct agttcatcat ttagactata caagtgacca gattcacgag gaagctgagg
     1921 tcttggaaca cactgtctct gacctgttcg tggaaagact agataaactg ctaggtttcc
     1981 ctaaaacctg cccccacgga ggaactattc ctgccaaggg agaactcctc gttgaaatca
     2041 ataacctccc actagctgat atcaaggaag ctggcgccta ccgcctgact cgggtgcacg
     2101 atagttttga cattctccat tatctggaca agcactcact tcacatcggt gaccagctcc
     2161 aagtcaagca gtttgatggc ttcagcaata ccttcactat cctcagtaac gacgaggatt
     2221 tacaagtgaa tatggacatt gcaaaacaac tctatgtcga gaaaatcaac taatttctca
     2281 agtcccctac caaccctgaa agttttattt tcagggtttt atttctatca ttttatttta
     2341 ctttcatttg gtggtagaat atttgaaaaa aatgggagag tatttgaaaa aaagaaaggt
     2401 agtttaacct atgaaaaaat cactaaaaat ttttgctaca tctaaatgat ttgacctctt
     2461 cggggttgct ttggtcgttg ggattgcgat tgcatctggt tatctcaact cacgcctcga
     2521 taaatttgta gactggggac tatggacagc tcttgtaccc tttggattga tttccgtaac
     2581 caacgttggg atttccatgt tgtccactcg tttcacgggg aaattaagca aatggggaaa
     2641 ttactttggt attgttaata ccattttgtc cggtgctatt gattatatcc ttagaaataa
     2701 ggcggccatc attacctatc ccgtcacctt actcatttat acctttgcta taaagaaatg
     2761 gaaagcttcg caagaaggca gacccaacca aatgagccaa aaacaggtaa aattggcggc
     2821 catcatcatt tccatcatcg ccttcctctt tgcctttgtg accaactata tcggatatgg
     2881 gggcaagatg aatctccttg cctacgtaac aactattgcc tttgcactgt ccctcattgc
     2941 caatgctttg aatgcattgg caatgagcga cagtggggct tttggttgat ttacaatttc
     3001 gttcagctga caaaagctgg tattcaagga aacttcgcca atatcggtaa atacatcttt
     3061 tatatcctca atgcaatcgg agctttattt gtctggaatg atgaagaagt tagataatag
     3121 aaggaaactc aaataaagaa tactagccaa cttaagagaa tctcccctct gctattttta
     3181 tcattttagt tgtaactccc attcttttag agtagaatga agttagataa aagagggagg
     3241 tcttgtcatg aaaaaactct atagaatcca ttttatagca attgcagtta ttgatttgct
     3301 actatttgca ttttttatcc caaatcattc atacctctct caactagatg taacttacaa
     3361 aac
//
LOCUS       DAVHIU010000147         3329 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVHIU010000147 DAVHIU010000000
VERSION     DAVHIU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.149"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..525
                     /locus_tag="VVN06_001912"
                     /pseudo
     CDS             1..525
                     /locus_tag="VVN06_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            629..1170
                     /locus_tag="VVN06_001913"
                     /pseudo
     CDS             629..1170
                     /locus_tag="VVN06_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1247..1468)
                     /locus_tag="VVN06_001914"
     CDS             complement(1247..1468)
                     /locus_tag="VVN06_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563398.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1433..2062
                     /locus_tag="VVN06_001915"
     CDS             1433..2062
                     /locus_tag="VVN06_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563399.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2098..3321)
                     /gene="pepT"
                     /locus_tag="VVN06_001916"
     CDS             complement(2098..3321)
                     /gene="pepT"
                     /locus_tag="VVN06_001916"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU5563400.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQMLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1105 a          642 c          648 g          934 t
ORIGIN      
        1 gttgttatcc tttccttaag tctgtgtgtt tatgctctga accaacatag ctaacaggcc
       61 aatacagata aaaatcgtgt ttcatatgta aacagtaata aagacactaa gaagactgaa
      121 aatttgactc cagactaggt tagtcaaaaa gaaggaattc aagcaaaaca aattgttatt
      181 aagattacag atcaaggtta tgtaacttct catggcgatc attatcacta ctataatggt
      241 aaagttcctt ttgatgcgct ctttagtgaa gagctattaa tgaaagcccc caactatcag
      301 ccgaaagatc aagatatcgt cagttaggtt aagggaggat atattattaa ggtagatgga
      361 aagtattatg tttaccttaa agatcaagct catgcagaaa atgtacgaac aaaagatgaa
      421 atcaatcgcc aaaaacaaga acatggtaaa gacgataaag gagctagtgc agaagtatct
      481 gtagcaaaat tacagggacg ttatactacc gatgatggtt atatatagtg agttgaattt
      541 agaatagcac atcatagttt ctaaatcatt gttagaaatt actttaaaat ccccttatca
      601 atttgctcat attctatttc aatctactat agtaccacat ggtggtcact atcatttcat
      661 tcctgagagt gacttgtcgg caggtgaatt agcagccaca tatgtcttta accctaatga
      721 tattgtcaga gatacaggtg atgcttatat agtaagacat ggggatcact atcactatat
      781 tcctaagtca tctcttaaca atcccccatc acactctaac acggaagagg taggaagttc
      841 ttctagttct gtattaagta atccgtcact acatgtccat catgaggaag aagatggtca
      901 tggttttgat gccaatcgaa ttataagtga agattcagaa ggttttgtga ttcctcatgg
      961 cgaccacaat cactacatca aggttcaaac taagggatat gaagcggctt tgaaaaacaa
     1021 gattccatcc ctgcaatcca actatcaacc tggaactttt gatgagaagg cagtcttggc
     1081 aaaagtagat caacttctag ctgatagcag aagcatctac aaagacagat tgagttagct
     1141 ttaggtcagt ttactgaaag tttgaaaaaa ataaaagtta gttgattgat tacttctcta
     1201 aattttgggc acactaaaaa gaggtcttga cacctaaact ttaaaattaa agcatattga
     1261 atccgtgctg ctgtgcgaag cgaatagcgt cttggacgaa cattgtctta tctggatttc
     1321 ttatttctgt aaggccttgt aatcttggat tataactctc ctgttttagg agtacatggt
     1381 agatggcaac caataatctg cgacagatag caaggaagaa gtagaaagtc ttttgagcaa
     1441 ggtaaatcaa ctcttggaac aaatccatga agaaggaatc agacaatcct tggcagaaga
     1501 agtagaaaat ctaaaagctg ccacaaacaa ggctgatgca gacttggatg aagtaaacag
     1561 tcaggtaaaa gatgtcttga ctcgtatcgc tagcgccctt caacaagaaa aggaaaatgc
     1621 tgagcaagat tctcagacac ttgtactcta tcaaaaactc tacgatattc tcatgtcgct
     1681 tcactcttat ttagaaagta ataatggatc tgatgcggac tttgataagg tggatgcatt
     1741 attagatcaa ctttctgcta agagtaaaga caaaacagct ttacttgaat cgacaaaagc
     1801 tattctagtt ttgaatcatc aaatccagtc aaaagctagc gcaagtgaag aaacaagtcc
     1861 agcaagaaat gctgaagcaa atagtgataa caccagtgct gagaaccaac caaacgcaac
     1921 tgcagaatct aacattgaaa ttgctagcga cgaaaacaaa ccaagcaata caagagattc
     1981 taaacctgct gaatcaactt cagaaaatga aacaacagaa tcttctacaa ctactggaaa
     2041 tcaagaaaaa ccagtagaat aaaaagaaag gcgaagtagc tgagctacct cgtcttttta
     2101 gcctttataa gctacgatgc caatgatggt atcaactgca cgttccatag tctgaaggct
     2161 aacgtattca aaacgtccgt gcatattttc gccacctgca aagatattcg gagttgggat
     2221 tcccataaag gaaatcttag agccgtctgt tccaccccgg attggttcga taataggcgt
     2281 gatacctaga tcttccataa cggctttagc aatggtaatt ggagtcatat ctttttcaat
     2341 gacttctttc atattgtagt actggtctgt caagttgaga gtgacacggt agctcccaag
     2401 ttctgcattc atcttatcag cgatagattg catggatgct ttacgcgctt caaaggcatc
     2461 tttttcaaaa tcacgaatga tgtagcttgc acgcacctcc tcaacactac ctgtcacatc
     2521 cattagatgg taaaaacctt ggtaaccttc agttaactca ggtcggtcat tttctggaag
     2581 ttgattatga aaatcaattg ctagctgaag ggcattgacc atctgctctt tggcagtacc
     2641 agggtggaca ttacggcctt ggaaatgcaa ttcagcacca gcggctgaga aagtctcgta
     2701 ctgaagttca cctagtggac caccatcaac agtgtaggca aaatccacat caaaatcttc
     2761 tgcatcaaat ttattggcac caacaccgat ttcttcatct ggaccaaaac caacacgaat
     2821 ctcacagtgc ttaatttcag gatgagcagt tagatattca atggctgtca taatttcagc
     2881 aattcctgac ttgtcatcag cacctagcaa gcttgttcca tctgttgtga tgagcatttg
     2941 tcctggatat ttttcaagac tcttgaagtc agctggatcg agtttgaaac cagaattccc
     3001 tagttcaatc acaccaccat cgtagttttc aattacctgt ggattgactc cttcagcatt
     3061 aaaatcagca gtatccatgt gcgatataaa accaatctta cgtgttaaag acggatcgtt
     3121 ggctggcaag gttccaatag caaaaccatt cggtagatag taaacatttt gcagtccaac
     3181 acgtttcatt tcaggaatta ggacatttgt tgcgaagtca acctgactct gtgtacttgg
     3241 agtagtagta gagtgttcat cagagcgcgt gttgacctta acgtaggtta agaagtggtc
     3301 caagagattt ggataagtca taaaaaaac
//
LOCUS       DAVHIU010000148         3321 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVHIU010000148 DAVHIU010000000
VERSION     DAVHIU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.150"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            413..574
                     /locus_tag="VVN06_001917"
     CDS             413..574
                     /locus_tag="VVN06_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563401.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            666..2345
                     /locus_tag="VVN06_001918"
                     /pseudo
     CDS             666..2345
                     /locus_tag="VVN06_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2613..3179
                     /locus_tag="VVN06_001919"
     CDS             2613..3179
                     /locus_tag="VVN06_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563402.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1255 a          421 c          715 g          930 t
ORIGIN      
        1 tatagaattt tgttacatca agataaatat tttctgatgg tcaaatttta aaaagattaa
       61 taactctcga taaattttga accttaacaa ttgaatagac caagacaagg cgttaattat
      121 ttttggagcg taataaccta tccgtcaaga tctttcggct gaataattcg gcaaaaaaag
      181 ttctgctaag caaaaagaaa gtgagcgata agtaagagtt tgaatatagg gagggatacc
      241 ctatccgcta tgaagaaaat gaggataatg atacttctaa ggttgaagcc ggctcatccg
      301 gaatagagga ggaggtgaga ttcctatgtt gctaatccaa agcacttgtc aatgtcaaaa
      361 aacttaatta aacaaataat tattagatat aaaactttag aggaggtata tattggacaa
      421 tgactggaat ggattggctg atttaatagc aaatctgatt gcaaaatatg ctggtgcttt
      481 agatttagat aatctacccg atccaacgcc agcaaaaaat caagagatga aaaatagttt
      541 tgacatggca aaaacacaaa ttgagacgga ctaataaaag tgttataata tacttgaaat
      601 gatagtccaa acttaaatta aaaaaattat atatgatata gaaaaattat attgaggtta
      661 atactatgtc aaaagaaaaa ataaaagtat acctctatac acgagtatct acgtcaatac
      721 agatagaggg gtattcgtta gaggcacaaa aatcacgaat gaaagctttt gctatttaca
      781 acgattatga aattgttgga gagtatgaag atgccgggaa gtctggcaag tctattgaag
      841 gtagaataca gttcaatcga atgatggaag atataaaatc tggaaaagat ggagtatctt
      901 ttgttcttgt gtttaagtta tcaagatttg caagaaatgc cgctgatgtt ctttctacac
      961 ttcagataat gcaggattat ggagttaatc ttatctgcgt tgaagatggc attgattcat
     1021 ctaaagatgc tgggaaacta atgatttctg ttttataagc tgtggctgaa atagaaagag
     1081 aaaacatccg tattcaaaca atggaaggtc gcattcaaaa agcaagggaa ggaaaatgga
     1141 atggtggctt tgctccgtat ggatataaac ttgaagacgg caagctgttt ataaatgagg
     1201 aagaggcagt tgcaataaga acgattttcg accagtatgt aaatactacg ataggagcta
     1261 atgggatatc taaatactta gagaatcatg gaattagaaa aatcccaaga cagaatggta
     1321 agaatccttt gtttgatgca ggtcttataa gaaagatatt aaagaatcct gtatataatg
     1381 ggaaaatagc atttggaaga agaactttag aaaaagttca tggtacaaga aatgaatata
     1441 agcaggttga acaagatgaa tatctaatat ctgaagggat acatgaagct atagtttccg
     1501 atgaagtttg gcaagctgct caggttaagc taaaatctca agcaaagaaa tatgagcatg
     1561 tgaataaagg aaaagataca cgcacacact tgctttctgg aattgtaaaa tgcccgatat
     1621 gtggagtggg aatgtttggg aacaagtgta tcaagaaaaa gaaagatggc acaaagtata
     1681 aagattttta ttactatggt tgtaaacata ggcagatgat aagaggtcat aagtgtacat
     1741 tcagtaagca aattagagaa gaattgttag atgatgctgt tgcagaggtg attgtcaaga
     1801 tagtaagtaa tccgaaattt gcttctatga tgcaagaaaa aatcaacatg aaggtggata
     1861 cctctgaaat agaaaaagaa atagataatt accaaaagga attgagaaag agtcattcta
     1921 caaagtttaa gctaattgag gaaatagata atttagatgt tgaagataag cattataaga
     1981 gaaggaaaca ggatttagat gatagactat atcgtatgta tgacaaaata gatgaattag
     2041 aatcatcact aattgatgcg aaagcaaaga aacagactat tgaagctgaa aagcttacag
     2101 gagataacat atataaggtt ctgatctatt ttgataagct ctataaagtc atgaatgatg
     2161 tagagcgtag gcagttaatt tcagctttga tttctgaaat tcaagtttat gaagaaaaac
     2221 aatctaacgg acaatggcta aaatccatta cttttaagct accaattata gaagaaaatc
     2281 tcaacatagg tttggacaat gatgaacaag ttgaatgtgt aagtttatta gagaaacgta
     2341 gttaatcctc aaaaggttcc ccgaaccttt tgagtcccac tgacgcatca cgctgaggcg
     2401 gtatcactgc ttgtacgagt tgaagcatca gcaaagtaaa agtagatgtt cagccaatgc
     2461 gacgaggtga gaccgaaggt cgataaggtg tgttctgggt ataggactcg taaagtaagt
     2521 aggaaatatg atgagatgat acagagagcc tgaattggtc aggtgacgtg cttatttaca
     2581 ttagcctgat aatgaaaatg gagagattat cgatggttat attggaattg taccaaaatg
     2641 attattctaa agatttagta gcatttgatt ccatagaaga tgggaaagcc tttgtagctc
     2701 aaatcccggg atacactctg gaaacagaag atggttttga agtggagtat tttaatccta
     2761 aaaacatacc ggattatatg gaaattattt ttaacggaaa tattgttcct ttatctagat
     2821 ttatgttcga tcctggggaa aatgtaaaca ttatttggaa agagatttca aatttatccc
     2881 tcaaaaatga tagagtgatc gaaggatatt caaaaattga tgcatatgtc gttaacaacc
     2941 atgaagttaa agcctatgtc gaaacaagag aaacgaacta tcgtaaagca aaagacttct
     3001 tagaaagccg cggatatgaa atcgacagaa gctttttcgg aagtgaagac ggtgaagcga
     3061 ttctttacag aaaaaaaggg atcgaagttt ggcatttctt gtgtcactta gacccaatgt
     3121 ttgtagagat tgaagatgta gaaggatatg ttaaagaaga agttggagag attcaatagt
     3181 caaccaaagt tccttgtgag tatatacctt tgctagtaaa agagtcaaaa cgttataacg
     3241 aaggggggga agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag
     3301 agtagccgct cataatttat a
//
LOCUS       DAVHIU010000149         3215 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVHIU010000149 DAVHIU010000000
VERSION     DAVHIU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.151"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(117..1532)
                     /locus_tag="VVN06_001920"
     CDS             complement(117..1532)
                     /locus_tag="VVN06_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU5563403.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     EEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1545..>3215)
                     /locus_tag="VVN06_001921"
     CDS             complement(1545..>3215)
                     /locus_tag="VVN06_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU5563404.1"
                     /translation="KNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERII
                     EYIAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLL
                     AYSASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILI
                     MVWLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQ
                     MYGVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGA
                     ASMAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGL
                     FAGLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEE
                     ESSSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAP
                     FDGKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIF
                     EMDLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
BASE COUNT         1084 a          649 c          441 g         1041 t
ORIGIN      
        1 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
       61 catatttaat gagatgttgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      121 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      181 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      241 cttttagaaa gttgtgcagt ggacatagaa acgcagtcaa tacatcccca agatgtatag
      301 cccataattt ccacaccatc ttgtagagct tcagctactt gcaataaatg ttcttccata
      361 tactgaattc tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga
      421 tctttagcac ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat
      481 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct
      541 aaataaggat ttactaagcc accaataata ttcccttctc ctgaattata ctgtgttgga
      601 agagcagatt gagtcacact catgtaatag ctaaaggata aaaaatctac agtataattt
      661 tttaataact ctgcatcttc agctgcaaac tctatgttaa tgtcatgttc cttaaaatat
      721 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      781 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      841 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      901 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
      961 ttctccttag gtatatctat tcctccacta gtaaacggta attccaaaac agagtttact
     1021 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1081 aatttttcat aaaaatgaat cattctccta tcaatccatc catgatattt tcttgctaaa
     1141 tataatggag tctcatagtg tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt
     1201 tcatcaaata attcatcata atatttcaac ccagcttcgt taggttcttc ttcatctcct
     1261 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa
     1321 aacaaggata tatcttcctt atatttatga taaaaatcaa tacctaacaa ttttaagtta
     1381 tcttctgtag gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact
     1441 gataatccct taccatcttc attatatgct ccctctactt gattagctgc aaccgctcca
     1501 ccccaaagaa aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt
     1561 aattccttag aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca
     1621 ttggtattcg ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc
     1681 aagtccattt caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta
     1741 ataaaacctt gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca
     1801 ccctcatcac tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca
     1861 tcaaatggtg cataaacctt accttcactt gggataatcg ctactccgtc tccaattagt
     1921 ttatctgaaa atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga
     1981 gaaaatatca tttttttatt tgaaactcca gcttcaactt ctaaattgct agaactctct
     2041 tcttcatcga ttccaaatat ataagctaat acaaaggtaa taacaaccga aatgaccgcc
     2101 acaattaaag catttacaat atttgatggc acatcagaat aaataaattg aggcaacgct
     2161 atcaaagatg ggacagcaaa tagatatgct ttaacactag taagacctgc aaataatccc
     2221 gctaatccac caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca
     2281 tataatgcag gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt
     2341 tgttttgtat tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct
     2401 aagtttgacc ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc
     2461 aaaaaaatag gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca
     2521 ccaagaatag ctaatgtaag ccatccagct acaccataca tttgcccaac tagatttgat
     2581 aatccttcac caacaattac tccaataggt ccgactacaa ctaaggcaat acagcttgat
     2641 actaataata ctagcgtagg ttgcaaaaaa ctcttagtaa tagctggtgt taatttagca
     2701 attatttttt caatatattt catcaaccaa accataataa gaattggaac gactgatgaa
     2761 ccataactag ctggtgtcac aggtgcacca aataaactaa gaggattccc tgattgcacc
     2821 atttgaacaa aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt
     2881 acttttaatt tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca
     2941 tccccaaaaa atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt
     3001 ggtaaaataa ttaccaagac tttcagcata cctcccccta acattgctgg aatgattgga
     3061 gtcatggaac cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtacccttga
     3121 ttaactgaat cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca
     3181 tcattaccaa gtataatttg atattgtcca ttttt
//
LOCUS       DAVHIU010000150         3188 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVHIU010000150 DAVHIU010000000
VERSION     DAVHIU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.152"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..710
                     /locus_tag="VVN06_001922"
     CDS             <1..710
                     /locus_tag="VVN06_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5563405.1"
                     /translation="LERILDEISKESNIPISYKTTGKVREFEEYIENDEVNQLYFLDI
                     DIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQVSALDFVDKDINDEMF
                     KKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILYIETTGVSHKLRIIGK
                     NFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPIS
                     RLKIRKLKDILEKKSQK"
     gene            762..833
                     /locus_tag="VVN06_001923"
     tRNA            762..833
                     /locus_tag="VVN06_001923"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Glu,seq:ttc)
     gene            839..912
                     /locus_tag="VVN06_001924"
     tRNA            839..912
                     /locus_tag="VVN06_001924"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:873..875,aa:Asn,seq:gtt)
     gene            complement(953..1495)
                     /locus_tag="VVN06_001925"
     CDS             complement(953..1495)
                     /locus_tag="VVN06_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU5563406.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            1602..2749
                     /locus_tag="VVN06_001926"
                     /pseudo
     CDS             1602..2749
                     /locus_tag="VVN06_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(2779..>3188)
                     /locus_tag="VVN06_001927"
     CDS             complement(2779..>3188)
                     /locus_tag="VVN06_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU5563407.1"
                     /translation="NINVSFPGNSQVSFESPTFYRLDTKTFTEAIQKIKEQPVTVSTS
                     KNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQTGFMKVDIPKGKGTITL
                     SFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
BASE COUNT         1048 a          481 c          595 g         1064 t
ORIGIN      
        1 gactagagag aatattggat gaaatttcga aagaatcgaa tattccaata tcatacaaga
       61 caacgggaaa agtccgtgaa tttgaagaat acattgaaaa tgatgaagta aatcagcttt
      121 atttcctaga tatcgatatt catggaattg agaaaaaggg atttgaagtg gctcagctca
      181 ttcgtcatta caatccttac gctattatcg tctttatcac tagtcgatca gagtttgcga
      241 ctctaaccta taaataccag gtatcagccc tagattttgt tgataaggat atcaatgatg
      301 agatgtttaa gaagagaatt gagcaaaata tcttctacac gaagagtatg ttacttgaaa
      361 atgaagatgt tgtagattat ttcgactaca attacaaggg aaatgattta aaaattcctt
      421 accatgatat tttgtatatt gaaacaacag gggtctctca taaattgcgc attattggta
      481 agaattttgc aaaagagttt tatggtacca tgacagatat tcaggaaaag gacaaacata
      541 ctcagcgatt ttattctcct cacaagtcat ttttggtaaa tataggcaat atcagagaaa
      601 ttgatcgaaa aaacttagaa attgttttct atgaagacca tcgttgtcct atttcaagat
      661 taaaaattag aaaattaaaa gatattttag agaaaaaatc tcaaaagtga ttgacaatta
      721 gcaagaaatt gatataatgg ttatatctgc tacagataga aggtccgttg gtcaaggggt
      781 taagacaccg ccttttcacg gcggtaacac gggttcgaat cccgtacgga ctattttatc
      841 cgccatagct cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc
      901 ctactggcgg agtatagata aaagggaaca cagctgtgtt cctctttttg tatcaatttg
      961 tatcaccaag cattttcata aggaagtctg ttatttcttg agaactttct ttttttccat
     1021 gtgcaatcca agtttggcag acaccaaaaa gtgcatgagt tagatagatg ctactatatt
     1081 ctaattcggt ggtatttaga ttcagttgca taaatcgctt ttgtaaatct gtactaagca
     1141 tgatatgaag tttatttcgt aagaaatttt ggatttcttt agtcccattt tcagaaagaa
     1201 gggcagccag aagtggttct gactctagat attcaaaaac ttctaaaata gcgtctcttt
     1261 tgtgatgagc atgtttttga aaaatatatt caaatgtatg gaatagcttg ctttgatagt
     1321 gctcaatcat atcatactta tccttatagt gagtatagaa gctggaacga ctaattccgg
     1381 ctttttctac taatttgaca gtagaaattt tatcaaatgg ctgttccatc agtaattgta
     1441 ccatagcatt ttcaatagtt cgctttgttt ttaagcgttt gttactttct tgcatatttc
     1501 ctccttgtaa acaaattaga ctatatgtct aaaaatagat tttttatctt gtaatttaga
     1561 ttttttaatg tataatctat tatatcaaaa ttttagacaa tatgtttaaa aaaggagaaa
     1621 ctaagtttaa agaatggaaa gcaattttaa aaaaaaccaa cctttattat tgtcatgatc
     1681 gggatttctc ttattccaga tctgtacaat atcatatttt tgtcatcaat gtgggatcca
     1741 tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga ggcttcctat
     1801 aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga aaataaaacc
     1861 ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga tggcgattac
     1921 tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt atccaatatt
     1981 caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat aagtgattct
     2041 gtatctcaaa atttaactga tagtattcaa tcggctcagt caattgtagc tttagtacaa
     2101 gatttacagg gaagtttaga aaacttacaa aatcaatcta atcaagtatc acctattact
     2161 tctacttctt tgataggatt gtcaagtgga ttaacagaga tacaaggaga tgttactagc
     2221 aaattagttc ctgccagtca gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt
     2281 gataaagttt ctcaggacgt catccagaga gccgttgggc ttggttgaaa tctcgagctg
     2341 aaataaatgg tattatagct gttttggcag gaattttggt atatggagga gttcatctta
     2401 ttggtttaac tgctaatcat gagatgagaa tatttattct catcatccta acaagtttag
     2461 tattcatgtc tatggtgacc actttaacaa cgtggaatag ccgtatagga gctttcttct
     2521 cacttatttt gcttttacta cagttagcat caagtgcagg tacttatcca cttgctttga
     2581 caaatgattt ctttagatct attaatccct ggttaccaat gagctattca gtttcgggat
     2641 tacgacaaac aatctccatc aacaagtcat tttcctagct gtcatactag ctctatttac
     2701 tggtttaggt atgctagcct atcaacataa gaaaatggaa gaagattaaa aaaatcgacc
     2761 gattaactgg tcgatttttt atgccttaga tgactttcgt ctgtgattat agattccaaa
     2821 tagtaagaga gaagtaaagg aacagattgc tccagtaata aaaccattgg gaatgaagga
     2881 aagtgtaata gttcctttcc ccttgggaat gtcaactttc ataaatccag tttgagcttg
     2941 tttaatttct attttcttac catcttggta ggcagaccaa cctttgtcat aaggaatggt
     3001 gaagaaaata gatgtatctt gttggacatc atatgtagca aaaaccttgt ttttagaagt
     3061 tgatactgtg acaggttgtt ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc
     3121 taaacgatag aaggtaggag attcaaatga tacttgtgaa tttccaggga aactaacatt
     3181 gatattga
//
LOCUS       DAVHIU010000151         3176 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVHIU010000151 DAVHIU010000000
VERSION     DAVHIU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.153"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVN06_001928"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVN06_001928"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5563408.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1400..2176
                     /gene="recX"
                     /locus_tag="VVN06_001929"
     CDS             1400..2176
                     /gene="recX"
                     /locus_tag="VVN06_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU5563409.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2265..2798
                     /locus_tag="VVN06_001930"
     CDS             2265..2798
                     /locus_tag="VVN06_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU5563410.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT         1018 a          579 c          560 g         1019 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaataatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggtcttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctcgag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtgataa gtaagtaatt ctgctaagcg
      841 attagcaatc acttgggttt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atacccctat cttatcacat
     1381 tttgagttat aatagagcta tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct
     1441 gatggagctt gataatggcg acaaatgcta tatcaccgaa gatacaattg ttcgttttat
     1501 gttatcgaga gataaggtga taagcgaaga ggaattgaaa gagattcaag actttgctca
     1561 attttcttat ggtaagaatc tggccctcta ccacttatcc tttaaagcac gcactgaaaa
     1621 agaagtcaga gaatatctga aaaaatacga tattgataaa aacatcgttt ctcaagttat
     1681 tgctaatctt aaagaagata agtggattaa tgatggtcag tacgcttatg ctatcatcaa
     1741 taccaatcaa ctttcaggag acaagggtcc ttatgtgctg actcagaaac tagctcaaaa
     1801 agggatttca aaatctacta tagaagagaa cttgaaagaa tttgattttt ctgaagttgc
     1861 tcaacgtgta gctaataaac tattgaaaaa atatgaggga aaacttccag ctcgtgcctt
     1921 gcaagataag attatccaga acttgactaa caagggcttc tcttattctg atgctaaaat
     1981 tgcctttgac gacttagata gccaagttga ccaagaaacg actcaagaac tcatcttcaa
     2041 ggaacttgat aagcaatata ctaagtatgc tcgaaagtat gaaggatacg aactgaagca
     2101 gcgtttaact caagttttag cacgaaaggg ctacgatttt tcggatatag caagcgctct
     2161 tagagaatat ctttaatatt tccaagtgaa attcacagat tttaggtaat tttatggtac
     2221 aatagtaaat gataaactta taaattgtag aaagttggtt agttatgaag cttccaaaag
     2281 aaggcgactt tattacaatt caaagttata agcatgatgg gagtctccac cgaacttggc
     2341 gggacaccat ggtactaaaa acaacagaaa acgccattat tggtgtcaac gatcatacac
     2401 ttgttaccga aagtgatggt cgtcgttggg tcactcgaga accggctatt gtttactttc
     2461 acaagaaata ttggtttaat atcattgcca tgattcgtga taatggaact tcttactatt
     2521 gcaatatggc tagcccctac tatctggatg aagaagcact gaagtatatt gattacgatt
     2581 tggatgttaa aatttttaca gatggggaaa aacgtctctt ggacgttgaa gagtacgagc
     2641 gtcacaaacg caaaatgaat tattctgatg acttggacta tattttaaaa gaacatgtca
     2701 aaattcttgt tgattggatt aacaatggac gtggtccttt ctcagaagcc tatgtaaaca
     2761 tttggtacaa gcgctatgta gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa
     2821 aatcctgacc tttttttggt agtataaaat aaattgtgta aacacaaaaa ggaataaatc
     2881 cattatagca gagttacaac acattactag aaagagattt attcctacga ctcagtttgg
     2941 catgcttaac ttcctaactc aaaaacaaag catcgattaa tttatccgtt cttcttttga
     3001 aacagatttg aactgataat tgctcgtttt aatttaagaa gctcttttga gcaaaacttc
     3061 atttttttaa gagtccaaca atttctgtct atcgcttttc tactagcaaa aaaacctacc
     3121 ttgaggcaag ttttcattta tatatctaat ttcgtaatag tatagtaaaa tgaaat
//
LOCUS       DAVHIU010000152         3173 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVHIU010000152 DAVHIU010000000
VERSION     DAVHIU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.154"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(314..1270)
                     /gene="lytA"
                     /locus_tag="VVN06_001931"
     CDS             complement(314..1270)
                     /gene="lytA"
                     /locus_tag="VVN06_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU5563411.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1646..3016)
                     /locus_tag="VVN06_001932"
     CDS             complement(1646..3016)
                     /locus_tag="VVN06_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5563412.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         1016 a          676 c          589 g          892 t
ORIGIN      
        1 tcataatttt ttaaataaaa aaattatgac aaaacaagga agcaatttat tgatgctgct
       61 taaaaatcta aaattgatga gttcctatca aaaatcgtat cttcaacctc aaaacagtac
      121 ttaaagctat ccgactcggt ttacattgtc aaatttagat tttatttgag cataactttc
      181 tagtttgctt tctgattttt gtttaatata gtagcaaaca ttgaaggaaa tatctccaca
      241 aaaaaacgca tactattaag ctttttcaag acctaataat atgcgctgtt ctgatttgaa
      301 agacattcca ttattatttt actgtaatca agccatctgg ctctactgtg aattctggct
      361 tgtctgccag tgttccgtct ggtttgaggt agtaccagcc tgttccgtcc gctgactgga
      421 taaaggcatt tgataccatg gcgccttctt tagcgtctaa gtagtaccaa gtgtccttgt
      481 acttgaccca gcctgtcttc atggcacctt cttcgttgaa atagtaccac ttatcagcga
      541 ttttcttcca gcctgtagcc atttcgcctg agttgtcgaa ccagtaccag ttgccgtctg
      601 tgtgcttcct ccagcggtct gcaagcatat agcctgaact gtcaaagtag taccaagtgc
      661 cattgatttt ctcaaacttg tcttttggat aagagccgtc tgaatgtacg taccagtagc
      721 cagtgtcatt cttctgccag cctgtttcaa tcgtcaagcc gttctcaata tcatacttaa
      781 actgctcacg gctaatgccc cattttgcca agtaagggta tggatccaca tggtctgagt
      841 ggttgtttgg ttggttattc gtgcaatact cgtgcgtttt aattccagct aaactccctg
      901 tatcaagcgt tttcggcaaa cctgcttcat ctgctagatt gcgtaagagt tcgatataaa
      961 ggcggtagtc cgtcatgaac tcttctttag ttgaatggct ttcaatcagt tcaaccgctg
     1021 cataggtctc agcattccaa ccgcccccaa cgtcccaggc accattatca acaggtccta
     1081 cctgcatgat gcaaccgttc ccaacaatgt gcgagaaaaa acctaattct gggtctttcc
     1141 gccagtgata atccgcttca ttctgtacgg ttgaatgcgg attcccagtt gagtgtgcgt
     1201 gtacttgcct atatggttgc acgccgactt gaggcaaatc tgttcttaat ttactcacat
     1261 taatttccat attctactcc ttatcaatta aaacaactca ttttttacaa tccagaacca
     1321 gaaactcctt tatttctacc ttacaaagaa gacaatctta gtcacgatta ggcttgtaga
     1381 tagaacttca aaacgcacta ttttgacact gtaaatagga ctgacaaggt ctgcattcta
     1441 tctacaataa caccccagac taaaaagctt ttcaaaagta tatttttaca gtctctatat
     1501 gtccttttca taaatactat actttattat atcatataaa agaagtcaaa agtctgttaa
     1561 actattttca acaccaaact aaagacgaga acacaagttt tttcgatttt cactagagga
     1621 aatggatttt attcagtaaa tccaactagg attgcacttt ggttgccaag attgccttgc
     1681 cttcttttat caagggatga cggaataatg agaagtacag ttgaactgtc atggcaaccc
     1741 agataagagg ttcacaaagg ataacaccct tatatcctgc ccaaggaata atcaaaacca
     1801 caaaaacgat ttttccgatt agttcaataa agctagaaac tagaggaagg atcttttgcc
     1861 ccaagccctg caagcaattg cgataaatca acaagaggct caaaatggga taaaaggttg
     1921 aactgatttg cagatagaga cttccatttt ctatcaagta accatctgtc gaactagcca
     1981 agaaggaaac caaagctgga ctggcaaaaa agaggaaaat acaaacaaaa actgcccagg
     2041 atatacttaa acgactgccg attcgaagac cttgaacaat acggtcaggt cgcttagctc
     2101 ctagattctg agaagcaaag gtcgtcattg atgcagaaat agcggtcata ggaagaaggg
     2161 caaaggtcat aatgcgtcga gctgccgtct gggcactaat aatcactgca ccaaatgtat
     2221 taacagaaaa ctgtaaaatc acactgccga tagatacaat tgaactcatc aagcccatag
     2281 ccaaaccttg ctccaagaga tccgcgtaca agcttttgtc ccatttgaaa tgtttaaact
     2341 gtggcaagag ttctggcaca cttttacgaa tataataaaa gcagagaacc gctgataaac
     2401 cttgcgaaat aatggtagca agtcctgcgg attgaactcc cagatgcaat tgcgtaataa
     2461 aatagagatc cagaaccaca ttaaccaagg cagagaaaat cagaaatccc agggctgcta
     2521 gactgtcacc aatagaccgc aacaagcctg caaaaagatt ataagcaaag ctgacaccta
     2581 cacaggtcac aatcatagaa atatattgat aagattgagg aagaatttct gcaggagtat
     2641 ctaagtattg caagagagga tacaagccaa gaaagcccag caacataact agaatgctca
     2701 aaagagcacc taaaatccag gtggctgcta ctgcttcctt gattttagtg aaattccgag
     2761 ccccataata acgagcaatg acaatcccca tgccattgcc aacaccaagt gtaaagccta
     2821 caatcaggtc aaaaatcgct gtcgtcgctc ctactgcagc caaggattct tgaccaagaa
     2881 atcgtccaac aatcaagaca tcagcagtgt tatagagctg ttgaaaaata tttgatagca
     2941 agattggaaa ggtgaagctt aagagcgagg gaagaatcgg accatgtatc aggtccactg
     3001 ttcgtttttt attcataaag ctattatatc agctttccag aggagcgaca agaaacagca
     3061 aacttatata tagcggaatg aaataaggac agtcaaatcg atttttaaca atgttttaga
     3121 agtagatgtg tactattcta gtttcgatct attatagttg gttgagtttg gaa
//
LOCUS       DAVHIU010000153         3168 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVHIU010000153 DAVHIU010000000
VERSION     DAVHIU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.155"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            436..2451
                     /locus_tag="VVN06_001933"
     CDS             436..2451
                     /locus_tag="VVN06_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU5563413.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTPVFSYTTFGNGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGNLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWFIFLRPVGEDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWYDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEDHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2453..3085
                     /locus_tag="VVN06_001934"
     CDS             2453..3085
                     /locus_tag="VVN06_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563414.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLTG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT          937 a          573 c          670 g          988 t
ORIGIN      
        1 gtcaaatgga gatgtttgat aaaatgcttt catagctaac caactgttga aaattatgag
       61 ttacaaaatc taaagtattt aagattagtt tataaaatta ggtctctgcc tgtagaaagg
      121 aacgtgcagt tagaaaggag gaaaaaatag gatactataa ttcagactga gatttctaat
      181 taaaatatgt agttgcatca tcccttgccg ttttggcatt tagtttggct cttcctgttt
      241 tggtagcttc tcaccgtggt ggcgagtgga cctatggagg ctatcatgat ccaaataatt
      301 ggggagcaaa ttacatttga tgcgggatgg taaagaataa ttgaagaaga ttcttttatc
      361 aatagtttag taagcttgtt agtttataga ggtcaggggt atttctgccc tctgttttag
      421 aataaggaat gcactatgaa aaaattattt atactcatat ccaacctgct tgctagtttg
      481 ttctttgttt gggtatttac catttggact gatacatatg tctcctatta ttaccccaac
      541 gttgttgtac gtgattcttc ccctgaaaca acttttcagc atgttgctac acgcttggaa
      601 aaactagcag aagaaacgga tagctttatt gcgattcaac accaagatcc taactcagaa
      661 ggtactccag tgttttctta tacgaccttt gggaacggaa agttgcctga tgggcttcag
      721 gaaaaaaatc tagaagatgc tcaaagtagt agtgttgaaa caaactattt tgtattcgat
      781 ggaaacctag atattcactt gctaagggag gagctaagtc aacttggctt gactaacatg
      841 cacctgacaa ttccatctaa actatctacg ttaatggcta tctttagtaa cggatttcag
      901 ttaatcagtc tattgatttt catcttaacc tttggagcac taactttaat tagtcaaatt
      961 agccaattac ggtcgtcggg cattcgcctc atttcaggag agaaacggtg gttcattttt
     1021 cttagaccag ttggtgagga tttgaaaggg atagctgttg gtttcagttt agctggcgtg
     1081 ctcaccattc ttatgcaaaa aattctgtcg cttccaacgc aatccctaat gaccatagga
     1141 gcaggcttgc tcagttataa tctcatcttg ttgtcgatct ccctgttttt cgctcaactc
     1201 ttcgcagttg ggataaaaaa gatacacctg atgcagatta taaaagggca agtgcctgtc
     1261 agaggaatca ttagtttgat tttaatcggt caactattag cgattattat tgtaacgctg
     1321 ggaataggga gtagcttaaa gtattcccaa gcctggcaac agcatcggat tggacaagag
     1381 gcttggagcc aggaaaggca actgattacc ctatcaatca gccgtgaagg aacgagtcct
     1441 ggttttgatg aacaagctca aaggaaactc agaacttggt atcaattgat ggatctggct
     1501 gtttcagaac aaaaggcttt cttatctaga caccagttaa ttgaccgtac tttgcaaaat
     1561 ggtatggctt cctccaaaaa ctttataacc tctacagaat ggtacgatta cagcccgaat
     1621 ggcaatgtcc ttatcgtcac accgcaatac ttggagcgtc aaaacattcc tgtagataca
     1681 actattgagc aaaagatgaa tcaccttgat gtaggggagt ttgtcttatt gctgcctgaa
     1741 cacctccgtt cagaggaaga tcattataaa tctgtttttg aagacgactt aaccagtcgc
     1801 atgtctagtc aagatgaacg acagcaaatg actgctacgg taggttattt agaatcaggt
     1861 caggatcgtt ttgtgtataa tacgacccct atttcttacc agcagttttt gaaagatcca
     1921 atcatcattg ttataacacc ccaatcaact ggtccacagt ccattttgtt ttggatagac
     1981 gcagtacaga actacgttct ctttaatcaa ttgtctgatg cccaggagct tatccagaga
     2041 caaggcattg aaaattgggt ctcagaaatg caaacaggtt accacaacta catcacatta
     2101 ttggataata tccagaggga acgttgggta atgctagcag gagctgtgct tgggattgca
     2161 acttcaatct tgttgtttaa cactatgaat aggctctact ttgaagaatt tagacgtgcc
     2221 atttttatca aacgcattgc aggtctcagg ttcttagaaa tccatcgcac ttatctcttt
     2281 gctcaactgg gtgtgttttt actgggattt gttgcgagtg tatttcttca ggtagagata
     2341 ggagttgctt tcttagtctt gttactcttt actggtctat ctcttttaca gttacatgtc
     2401 caaatgcaga aagaaaacaa gatgtccatt cttgttttga agggaggtta atatgattga
     2461 acttaaacag gtgagtaaat cttttggaga acgagagtta ttttcgaatc tttcaatgac
     2521 atttgaggct ggaaaagtct atgccttaat tggttcaagt ggtagcggaa aaacaacctt
     2581 gatgaacatg attgggaaat tagaacctta tgatgggacg attttttacc gaggtaaaga
     2641 cttggccaat tataaatcga gtgatttttt ccgtcacgaa ttgggttacc tcttccagaa
     2701 ctttggctta attgaaaacc aaagtattga agaaaacctt aagctaggtc tcactggtca
     2761 aaagttgagt cggtcggaac agcggttgag gcagaagcag gctttagaac aggtcggcct
     2821 ggcttatctt gacctagata agcgcatctt tgagttatcg ggcggagaat cgcaacgggt
     2881 tgccttggca aaagttatct taaagaatcc accctttatt ctggcagatg agccaacagc
     2941 ttcaatagac ccagcaacct ctcagttgat tatggagatt ttgctatctc ttcgagatga
     3001 taataggcta atcattatcg caacacataa tccggcaatt tgggagatgg ctgatgaagt
     3061 gttcacgatg gatcatctga aataaaaatc cttgttttta attgcacgat gagttactga
     3121 aatattatca tgaatcaaga attggagtta atttagaatt gtacttta
//
LOCUS       DAVHIU010000154         3149 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVHIU010000154 DAVHIU010000000
VERSION     DAVHIU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.156"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            422..844
                     /locus_tag="VVN06_001935"
     CDS             422..844
                     /locus_tag="VVN06_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5563415.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            841..1071
                     /locus_tag="VVN06_001936"
     CDS             841..1071
                     /locus_tag="VVN06_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5563416.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            1532..1735
                     /locus_tag="VVN06_001937"
     CDS             1532..1735
                     /locus_tag="VVN06_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU5563417.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            1817..3034
                     /locus_tag="VVN06_001938"
     CDS             1817..3034
                     /locus_tag="VVN06_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5563418.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         1109 a          540 c          635 g          865 t
ORIGIN      
        1 tatttaataa aaagcactac taaacgccaa taaaaaaaac cgttatatgg tagtgctatt
       61 tacgctgtta aaatattgta tattacttcc aaatggcggt ttgttggagg tcaacgtcgc
      121 catgaagtac atcatataca ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt
      181 ctatctgcaa tagataagta cgtccaccaa tgtggtttta taaatcatat agatagaata
      241 acagaagcat gtaaacagag aaataaatct gtttatatgc ttttttggct attcagaact
      301 tttttacaaa gtttatttat cagtaatgca acaaatcccc ctttcacatt gggactaaga
      361 gtgaaaggag ataaacgagc aaggctcact tcctttccta gacagaaagg gggtgagaaa
      421 catgaaacca tcttctttcc agaccacaat agaaaatcag tttgactata tctgtaaacg
      481 tgctatggaa gacgagcgaa agaattatat gctttatctt tcaaggattg caaagcgtga
      541 ggtgtccttt tcggatgttg gcgattatct tgttagccag tttgcgacaa cagataacta
      601 ttcaactgac tttcagattt ttacactcaa tgggttatca gtaggcgttg aaaatgattt
      661 gttgagtgaa gcattacgtg agttgccaga caagaaacgt gaaattctac tgctgtttta
      721 ctttatggac atgagcgatt cagaaattgc agacctgttg aaattgaacc gttctactgt
      781 ctatcggcat agaaccagtg gactagcctt aattaaaaag tttatggagg aatttgaaga
      841 atgaaaacac aatatcctat gattcccttt cctctcattg taaaggcaac agatggcgat
      901 accgaagcga ttaaccagat tctacatcat tacagagggt acataacgaa gcgttcccta
      961 cgacttatga aagatgaata tggcaatcaa agtatggtcg ttgatgaagt cttacgtgga
     1021 agaatggaaa ccagactgat tacaaagatt ttgtcatttg aaattaagta atatcctctc
     1081 tcctttcgtg gaagcgtgct aaaccattcc acgcttcccg aacagggagg tttgttattc
     1141 caccaaagca tattgagctt tcaatgtgtt ttgataggct aacgagccat tgttctttga
     1201 aaactgaata aaagtaatcg aatacgtttc gataagaaaa gagccaacgg aactaaccgc
     1261 catgacctat cttataaaga tagcgagcga ttcatgttag tgatccgaga agcaatcttt
     1321 agcaggattg cctgcaacga cattcttatc gtgataatga tactcccata cagtcaatag
     1381 tccgagcgtg ataaaaccgt cgcaggcaat gagtatggct acatgagaac catgcagggg
     1441 tggaactccc gtgagctttg ctaaagctgt tcgattgctg gtaaaacaac ttttatgaaa
     1501 tccaaataag tgatttggaa aggaggattt tatgaagcag actgacattc ctatttggga
     1561 acgttatacc ctaaccattg aagaagcgtc aaaatatttt cgtattggcg aaaacaagct
     1621 acgacgcttg gcagaggaaa ataaaaatgc aaattggctg attatgaatg gcaatcgtat
     1681 tcagattaaa cgaaaacaat ttgaaaaaat tatagataca ttggacgcaa tctagcgtcg
     1741 ccaaagggtc ttgtatatga taaaatagta ttaagtcgta tcaaggctct ttccataaag
     1801 gaaaggagca aatgccatgt cagaaaaaag acgtgacaat aaaggtcgaa tcttaaagac
     1861 tggagagagc caacgaaaag acggaagata cttatacaaa tatatagatt catttggaga
     1921 accgcaattt gtttactcgt ggaaacttgt ggctacagac cgagtaccag caggaaagcg
     1981 tgattgtatc tcacttagag agaaaatcgc agagttacag aaagacattc atgatggtat
     2041 tgatgttgta ggaaagaaaa tgacactctg ccagctttac gcaaaacaga acgctcaaag
     2101 accaaaggtt agaaaaaaca ctgaaactgg acgcaaatat cttatggata ttttgaagaa
     2161 agacaagtta ggtgtaagaa gtattgacag tattaagcca tcagacgcta aagaatgggc
     2221 tattagaatg agtgaaaatg gttatgctta tcaaaccatc aataactaca aacgttcttt
     2281 aaaggcttca ttctatattg ctatacaaga tgattgtgtt cggaagaatc catttgactt
     2341 tcaactgaaa gcagttcttg atgatgatac tgtccctaag accgtactaa cagaagaaca
     2401 ggaagaaaaa ctgttagcct ttgcaaaagc tgataaaacc tacagcaaaa attatgatga
     2461 aattctgata ctcttaaaaa caggtcttcg tatttcagag tttggtggtt tgacacttcc
     2521 agatttagat tttgagaatc gtcttgtcaa tatagaccat cagctattga gagatactga
     2581 aattgggtac tacattgaaa caccaaagac caaaagtggc gaacgtcaag ttcctatggt
     2641 tgaagaagcc tatcaagcat ttaagcgagt gttagcgaat cgaaagaatg ataagcgtgt
     2701 tgagattgat ggatatagtg atttcctctt tcttaataga aagaactatc caaaagtggc
     2761 aagtgattac aacggcatga tgaaaggtct tgttaagaaa tacaataagt ataacgagga
     2821 taaattgcca cacatcactc cacatagttt gcgacataca ttctgtacca actatgcaaa
     2881 tgcaggaatg aatccaaagg cattacagta cattatggga catgctaata tagccatgac
     2941 gctgaactat tacgcacatg caacattcga ttctgcaatg gcagaaatga aacgcttgaa
     3001 taaagagaag caacaggagc gtcttgttgc ttagtagtac aaatgaattt actacttatt
     3061 taccacttct gacagctaag acatgaggaa atatgcaaag aaacgtgaag tatcttccta
     3121 cagtaaaaat actcgaaagc acatagaat
//
LOCUS       DAVHIU010000155         3110 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVHIU010000155 DAVHIU010000000
VERSION     DAVHIU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.157"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(58..312)
                     /locus_tag="VVN06_001939"
     CDS             complement(58..312)
                     /locus_tag="VVN06_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU5563419.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(332..2173)
                     /gene="typA"
                     /locus_tag="VVN06_001940"
     CDS             complement(332..2173)
                     /gene="typA"
                     /locus_tag="VVN06_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5563420.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2335..3060
                     /locus_tag="VVN06_001941"
     CDS             2335..3060
                     /locus_tag="VVN06_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU5563421.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDSGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          906 a          695 c          600 g          909 t
ORIGIN      
        1 aaataaattg caatcttgta aaattcctag caaaaaagag agccaaaact ctctttttta
       61 tcttctttta ctttttttga ctggcatgag tgtgatgtct ctcacactaa agtaagctag
      121 gatcaacatg gctattgcta ggaatatttc tgttggtaat tgaaaaattt tcagaaaaga
      181 tagaaccaat aaaatcaaga gtgccactaa aatacatacc atagcgacga tattgactgt
      241 ccctttaatg ctttctggtg tcgcaaatac atagagtagg agcagtaaaa ttcctaggac
      301 taaatagacc atctttctct ctttctagct cttattcagc tgattttttc ttcttgttag
      361 ctttctcacg ctctgctttg ttaaggattt gtttacgcaa acggatagac tcaggcgtta
      421 cttccatgta ctcatcgtcg ttcaagaact caagagactc ttcaagtgtc aagatacgag
      481 gcgtcttgat aacagctgtt tggtccttag tagctgaacg aacgttggtc atttgttttg
      541 ccttcgtgat gttaactgtc aagtcatttt cacgagagtt ttcgccgatg atcattcctt
      601 cataaacctc agtacctggg ttgacaaaga tcgtaccacg ttcttcgata gacatgattg
      661 agtaagttgt agccttacca gcatcgatag aaacaagggc accacggtga cgtccaccaa
      721 tttcccctgg aatcaatggc aagtactggt cgaaggtatg gttcatgata ccgtaaccac
      781 gagtcattga caagaactca gttgagtatc caatcaaacc acgcgctgga acaaggaaga
      841 ccaaacgagt ttgaccatta ccagttgaaa tcatatccaa catttcacct ttacgttcag
      901 aaaggctttg gataacagac ccttggtatt cttctggagt gtcgatttgt acacgttcaa
      961 atggttcaca tttaacaccg tcgatttctt ttacgataac ttctggacga gatacttgaa
     1021 gttcatagcc ctcacgacgc attgtttcga taaggattga caagtgcaat tctccacgtc
     1081 ctgaaacagt ccatttatct ggtgaatcag ttgggtcaac acgaagggaa acgtctgttt
     1141 gcaattctgc ctgcaagcgt tcttccacct tacgagaagt tacccattta ccttctttac
     1201 cagcaaatgg tgagttgttg accaagaaag tcatttgaag agttggctca tcgatgtgta
     1261 ggattggaag agcttctact gcatctgtcg gagtgatggt ttcaccgaca aagatgtctt
     1321 ccatacctga aacggcaatc aagtcacccg ctttggcttc ttggatttca cgacgttcca
     1381 aaccaaagaa accgaagagt tttgtaacac ggaagttttt agttgtaccg tcaagtttag
     1441 aaagggtaac ttggtcccca accttaactg taccacggaa gacacgaccg ataccgatac
     1501 gtccaacgaa gtcattgtag tccaaaagtg acacttggaa ctgcaaaggc tcatctgagt
     1561 tatctactgg agctgggata tggtcgataa tcgtgtcaaa gattggtgcc atagtcgctt
     1621 cttggtcagc tggatcatct gacaatgaag aagttccgtt gatcgctgaa gcataaacca
     1681 ctgggaaatc aagctggtcg tcatctgcac caagctcgat gaaaagttcc aagacttcat
     1741 ccactacttc tgctggacga gctgatggct tatcgatttt gttaacaacc acgattggga
     1801 caaggtcttg ttccaaggct tttttcaata cgaaacgagt ttgtggcatg gttccttcat
     1861 aggcatctac gaccaagaca acaccgtcaa ccattttcat gatacgctca acttctccac
     1921 caaagtccgc gtgtcctggt gtgtccataa tgttgatacg agttccgttg taagcaacgg
     1981 cagtattttt agcaaggatg gtaatcccac gctctttttc gatatcgttt gagtccatag
     2041 cacgctctgc caattcagta cgtgcatcaa gcgtttctga ttgtttcaat aattcgtcaa
     2101 ccagggttgt tttaccgtgg tcaacgtggg cgataatcgc aatgttacgg atatcttctc
     2161 ttaattttgt catgatttcc tctataatat tcaaaattta ttttctaact gaacgattat
     2221 accataattt caagtaaata acataactca agcaagtgta aatgttttca ctccactttt
     2281 cttttcacgt caagcctttt caaagcaagc gacttatgat aagataggca cagtatgcgt
     2341 ttagataatt tattagctca agaaaaaatc agccgaaagg ccatgaaaca agccttactc
     2401 aaaggggaaa ttctagtcga tggttgccca gcccgatccc tagctcaaaa tatcgataca
     2461 ggactacaag aactcctttt tcaggatcga atcattcaag gctatgaaca cacctatctt
     2521 atgcttcata agcctgctgg tgtcgttaca gccaacaaag acaaggaact tccgaccgtc
     2581 atggacctgc ttccatctga catccagtct gacaagctct atgccgttgg ccgactggac
     2641 cgagatacaa cgggactcct cctcttgacc gatagcggtc ccttgggctt tcagctcctc
     2701 catccccaat atcatgtcga taagacttac caagtccagg ttaatggact tctaacacct
     2761 gaccatatcc aagcctttca aaagggaatt gtctttttag atggtactgt ctgtaaaccc
     2821 gcaaaactag agattctatc tgcaagtccc tccctcagtc aagcctctat caccatttca
     2881 gaaggaaaat ttcatcaaat caagaaaatg ttcctctcgg ttggtgttaa ggtgactagc
     2941 ctcaaaagaa tccaatttgg ggacttcaca ttgaacccag atttagcaga aagtaactac
     3001 cgccctttga accaaaaaga gttacaaatc attaaaaact atttagagat gagtcgataa
     3061 aacaaaaaaa gctttaaaac taaagctttt ttcttttatt taccgaaaaa
//
LOCUS       DAVHIU010000156         3091 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVHIU010000156 DAVHIU010000000
VERSION     DAVHIU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.158"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(282..2783)
                     /locus_tag="VVN06_001942"
     CDS             complement(282..2783)
                     /locus_tag="VVN06_001942"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5563422.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAYLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVTTDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYIDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT          848 a          689 c          608 g          946 t
ORIGIN      
        1 aaactttcct tgtcccaaag acaattttta aaataatatg cacccactat tgacattact
       61 aatactataa ctgacactac aataatagaa atcttaaata ataaaatttc acacttcctg
      121 ctatcgttca ctaatctatt cattctgacc tattctaaaa gtaactctaa agttcatact
      181 ctagagttac ttctttttat ttcacaacaa tgttaactaa tttagttggc ttcaatagtc
      241 cagtggacta ttgaaggtgg cagatagagc taataaactc gttatttaac gacgatatta
      301 acgagtttat tcggtaccgc aattactttc acgatttcct taccgtcaat ttctgctttg
      361 actttttcat cagctaaagc gatttcttgt aattcttcac gtgatagatc tttagcaacc
      421 atgagtttgg cacgaacttt tcctttgatt tggacgacaa tttcaatttc atcttcaacc
      481 aatttgcttt cgtcccaagt tggccaagct acataagaga ttgactcacc tgtttctgcg
      541 actgtttgcc agagttcttc tgccaagtga ggtgcaaatg gtgcaatcaa ttgaataaag
      601 cctttggcat agtcaatata aagcttatct tccttgttag cagcattgac aaagaccata
      661 agttgggcaa tagctgtgtt gaatttgaga gactcaattt gctcagtaac agctttgact
      721 gtttcgttgt aagccttgtc aagagcacca ttgttttccg caaggatttc tttacttgta
      781 atcaaacggt aaactcggtc aaggaactta cggcttcctt ccaaaccttc ttctgaccaa
      841 gcaatcgaag catcgagtgg tcccataaac atttcataaa cacgaagggt atcggcaccg
      901 tattgttcca ccacatcgtc tgggttaaca acgttcttga gcgatttaga catcttggct
      961 ggcgcttgct ccaactcttc ccctgtttct acatggaaga aggaaccatc acgtttttca
     1021 accttgtcgg ttgtcacaag agcaccacgg tggtcacggt agcttgttcc caaaatcatc
     1081 ccttggttaa agagtttttg gaatggttcc ttagtcggaa caacaccgag gtcatagagg
     1141 aatttatgcc agaaacgagc ataaagcaag tgaagtacag catgttccgc accacccacg
     1201 tagatatcta ctggcaacca ttgtttgagg aggtcctcat cagccaattt ctcagtattg
     1261 tgcgggtcaa tatagcgaag gtagtaccag cttgaaccag cccattgtgg catggtgtta
     1321 gtttcacgac gacctttgac accatcttca cgagtcactt caagccagtc tgtcaagtta
     1381 gctagtggac tttcaccggt acctgaagga cggatgtcct tggtgacagg caagacaagc
     1441 ggcaattcag tttcaggaac agctgttgaa gttccatctt cccaatgaat aattggaatt
     1501 ggttcacccc agtaacgttg acggctaaag agccagtcgc ggagacggta ggtaaccttc
     1561 tcctgaccac agcctttttc ttccaagtaa gccacaatct tggcaatagc gtcttctttg
     1621 ttcaatccat ctaggaagtc tgaattgaca tgcaggccat cctctgtgta ggcagcttct
     1681 tcgacatttc caccttcaag tacttcgacg attggaaggt caaattgttt ggcaaattcc
     1741 cagtcacgtt ggtcgtgggc aggcacagcc ataaccgcac ctgttccata actagcaaga
     1801 acatagtccg caatccagat tggcatttcc ttaccattga cagggttgat ggcataagca
     1861 ccagtccaaa caccagtttt ttccttagca aggtctgtac gaaccaagtc agacttaagg
     1921 ctggcttggt gtttatagtc tgctacagct tctgcttgct ctgaacttgt gatagcgtct
     1981 actaattcat gttcaggagc caagacagtg aaagtcgcac cgaaaagtgt gtccggacga
     2041 gtagtaaaga ctgtaaattc cttgtctgtt ccttttactt tgaaagttac attggcacca
     2101 gttgatttac caatccagtt gcgttgcata tccttgatag actctgacca atctagttca
     2161 tctaagtcat tgagcaagcg ctctgcgtaa gccgtgattt tgagcatcca ttggcgcatt
     2221 ggtttgcgga caactggata gcctccacgc tcagaagttc cgtcaggaag cacttcttca
     2281 ttggcaatgg cagttcccaa ttcctcaacc cagtttactg gcacttcagc ttcataggcc
     2341 aagccttttt cgtaaagctt ggtgaaaatc cattgagtcc acttgtagta gtttggatct
     2401 gttgtgttga cttcacgatc ccagtcataa gaaaatccaa gcgcattgat ctgacgtttg
     2461 aagttggcaa tgttttccgc tgtaaattct gctgggtcat taccagtatc catagcgtat
     2521 tgctctgcag gcaaaccaaa agcatcccaa cccattgggt gaaggacatt gtagccttgc
     2581 gcacgtttgt aacgactgag gatatcggtt gcagtataac cttctgggtg tcctacgtgc
     2641 agaccagctc cagacggata agggaacata tcaagcgcat aaaacttagg ttttgatgca
     2701 tctgttcctg tcttaaatgt atgatgttct gcccagtagc cctgccactt aggctcaatt
     2761 tctttatgat tataaaaact catggtctct ctccaatttt gtgatgttac tattatacca
     2821 tttttgaggg aatttgaaat gtgtttaaag aaaaaactgt atactgtatc tcattaattt
     2881 tgatgactat gaattgctct atctcccact ttttatccca ttgttatgca gtaaatcaag
     2941 gagtaagtca agaagcttat ctcgtatagc acaaaatact tattttagac cgaagataaa
     3001 actggaatgc taattaatgt attcacaata aatagccgac tacctatgtc gctttttgat
     3061 ttatgaagaa cgtatagtag actgaatctg a
//
LOCUS       DAVHIU010000157         2986 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVHIU010000157 DAVHIU010000000
VERSION     DAVHIU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.159"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(162..890)
                     /locus_tag="VVN06_001943"
                     /pseudo
     CDS             complement(162..890)
                     /locus_tag="VVN06_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1150..1461)
                     /locus_tag="VVN06_001944"
     CDS             complement(1150..1461)
                     /locus_tag="VVN06_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563423.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1476..2762)
                     /locus_tag="VVN06_001945"
     CDS             complement(1476..2762)
                     /locus_tag="VVN06_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU5563424.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          892 a          656 c          526 g          912 t
ORIGIN      
        1 tttaatttga taggctttaa tacctaagct ccgactttat cacttaaaaa ataaaaaagg
       61 acagctaatc tcttgcgcat caagggattt cgccattctt taatctcgtt taagtgttgt
      121 atatggagct ttgcataatt ttttaaaact aaacaactac attaagcacg aactgtgacg
      181 ctggttccat cttccttata aagattgata agcccctctt taagagctcg aaccatgtct
      241 ccagcttgaa caggttgtgg gactttaata gtcaatagtt ccattggatt tggagcgcgg
      301 tcgattttat tgcctttggc atcatgcaaa tcttcaatat aggtttcaaa atgacggaaa
      361 cctggaccat aaaactcaac ttggtcccct tcgttaatga cgtttcgttg acgaatagtt
      421 gctgtttgtg ccgcatcatc ataagaaacc acttcagcga caaacttgta ctcagggatt
      481 ttacgacgag caccaaacaa ctgctcattt tcagatggtg taccatagta aaatcctgta
      541 gccagttcac gttgggcaac cttccacatc tcgtccacca agtcttgttt gatagcttca
      601 aacttttcag gactttcaag ataggcatcc acagccgcct tgtaacagtt ggttactgtt
      661 gatacatagt gaatagactc catacgtcct tcgattttta gactgtccac accattttca
      721 atcatatctg agatatggtc aatcatagac atatcaacgg ctgacattga aaattcttct
      781 ggaatctcac cctgcaaact cttacgttct ttcccaaatg gcatatcgta aaggtcgtac
      841 ttccaacggc atgactgaga acatccacca cggttggcat cacgcatact ctagacggat
      901 tgttttgaca attctaacct tttagacttt gcataataac tgttaaacag tcgaaaatta
      961 ccccagtttt aacaagtagt caaaatacca gataaagtaa aaaggggcta ggaaaaaagt
     1021 ctttaaaatc ttaaaaacgc atagtatcag gtattgaaca atcttgatat tatgcgtttt
     1081 attgtgggaa gatttacttc attttctctt ggaattgagt tttctcctac ttcaaaacta
     1141 gatgatttgc tatttaaaat agcgttcctt atgattttgg caacctggat taaagcgaga
     1201 aaaacaaaag aggcaactta agctttcttt atattgctta tatgttaata gttttagaca
     1261 aacaccacat aaaataggct tatcctgtat caaagataag ggataaggct caaaagggtg
     1321 attttctaat gaatcatgac accgataaca agcataatac tttttacaat catagcattg
     1381 taaagcaata atatccagct tgctatggta atgaatgcat cttgtttgct tatccactaa
     1441 gaaaccctgt gcctctatca tttccttttc ctttcttaag ctcgaactgt gacgctggtt
     1501 ccatcttcct tataaaaatt gatgagtcct tcttttaatg cacgaaccat gtctcctgat
     1561 tgaacgggtt gtgggacttt aatagtcaat agttccattg gatttggagc gcggtcgatt
     1621 ttattgcctt tggcatcatg caaatcttca atataggttt caaaatgacg gaaacctgga
     1681 ccataaaact caacttggtc cccttcgtta atgacgtttc gttgacgaat ggttgctgtt
     1741 tgtaccgcat catcataaga aaccacttca gcgacaaact tgtactcagg aattttacgg
     1801 cgagcaccaa acaactgctc attttcagat ggtataccgt agtagaaacc tgttgccaat
     1861 tcacgttggg caaccttcca catctcgtcc accaagtctt gtttgatagc ttcaaacttt
     1921 tcaggacttt caagataggc atccacagcc gccttgtagc agttggttac tgttgatacg
     1981 tagtgaatag acttcatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata
     2041 tctggaatgt ggtcaatcat agacatgtca acggctgaca ttgaaaattc ttctggaatc
     2101 tcaccctgca aactcttacg ttctttccca aatggcatat cgtaaaggtc gtatttccaa
     2161 cggcatgact gagaacatcc accacggttg gcatcacgca tactcatgtg gtttgaaaga
     2221 gtacaacgtc cagagtatga aatacacata gctccatgga caaaggcttc aatttcaaca
     2281 tctgtacgtt tgcggatctc agctaattct tccattgaaa cctcacgcgc taaaacgaca
     2341 cgagtcaagc ctagctcttt ccagaactca agggtttcat agttagtggc actggcttgg
     2401 gtagaaaggt ggatttcaag gcctggtgct tcagtcgctg caatcataat caaggctggg
     2461 tcagatacga taactgctgc aatcccgata tcacgcagtt tacggaacca ctcaccagca
     2521 ccagcttcat ttccttcgtg cataaccata ttagccgcta catagacctt ggcaccatac
     2581 ttggccgcaa actgcacgcc ttcttccatc tgttcgaaag taaagtttcc cgcacggcta
     2641 cgaagaccat aggcctgacc accgataaag acagcatctg ctccatactg aacagctacc
     2701 tttagcttct ctaaagtccc tgcaggtgat aaaacctcag gacgttttaa tgtttttgtc
     2761 atgatttctc ctatttgcaa tataaaagtt tgacattttt ccttctcatt ttatagtaaa
     2821 taaaggataa aatcaagcct agacggattg ttttgacaat tctaatcttt tagactttgc
     2881 ataataactg ttaaacagtc gaaaattacc ccagttttaa caagtagtca aaaagtctaa
     2941 aaaagtaaaa accagtatcc caagatactg actcgtaaaa atacat
//
LOCUS       DAVHIU010000158         2912 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVHIU010000158 DAVHIU010000000
VERSION     DAVHIU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.160"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(311..1621)
                     /locus_tag="VVN06_001946"
     CDS             complement(311..1621)
                     /locus_tag="VVN06_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5563425.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1858..2730
                     /locus_tag="VVN06_001947"
     CDS             1858..2730
                     /locus_tag="VVN06_001947"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU5563426.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          880 a          500 c          670 g          862 t
ORIGIN      
        1 ttgactatcc tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt
       61 atgacgaagt gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg
      121 aatccatcga taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg
      181 atgagattta cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact
      241 tttcctcgtt tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta
      301 gataataaat ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca
      361 aacttaagct gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct
      421 ctatattggg aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag
      481 tagatagagc gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt
      541 gagcactgat gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac
      601 taaagagtga catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa
      661 tggttgttgt catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca
      721 ttagtaagct catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg
      781 cttcttgctt ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc
      841 caatatcaaa tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca
      901 acaagggaaa aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt
      961 gaaccttgct gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac
     1021 tatagtgact gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga
     1081 taaagatatg attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc
     1141 cttgaaagac aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat
     1201 tgacggcttg agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta
     1261 gaggacagca aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg
     1321 ccatcaggta taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt
     1381 gttgagccaa aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc
     1441 taaaaatggt aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc
     1501 ccatgagagt ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt
     1561 tggtgcggta accaattcca atggctagag caatggcgct aatcattatt aaatgaatca
     1621 ttgaattgtc ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac
     1681 cacctgtttc ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa
     1741 ttgtattgac acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa
     1801 gttaatagta attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg
     1861 tcaatcattt caactgattt aacccctttt caaatagatg atacattgaa agcagccttg
     1921 cgagaagatg ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa
     1981 gccaaggtgt cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa
     2041 agggttttta ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat
     2101 ggggatcgtt tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc
     2161 ttaacatgtg aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg
     2221 acagctgctt atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa
     2281 actactccta atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat
     2341 catcgcttta atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt
     2401 agtgttcaga aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag
     2461 gtggaagtgg aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc
     2521 atgttggata atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt
     2581 tctcggattg aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta
     2641 gcgattgatt acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc
     2701 atgaagggtt taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat
     2761 gtctttcctc tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa
     2821 aatgaaccaa aaatagtaca caatgtggta taatcttctt atggcatatt caatagattt
     2881 tcgtaaaaaa gttctctctt attgtgagcg aa
//
LOCUS       DAVHIU010000159         2890 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVHIU010000159 DAVHIU010000000
VERSION     DAVHIU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.161"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            3..797
                     /locus_tag="VVN06_001948"
     CDS             3..797
                     /locus_tag="VVN06_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563427.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQYVLK"
     gene            801..998
                     /locus_tag="VVN06_001949"
     CDS             801..998
                     /locus_tag="VVN06_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563428.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            988..1539
                     /locus_tag="VVN06_001950"
     CDS             988..1539
                     /locus_tag="VVN06_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563429.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNLEFPSMIELFSIL
                     PEYPLKKDGREISLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSTI
                     SSNHVSFHLQPQNSVLSSLRLAS"
     gene            1598..1858
                     /locus_tag="VVN06_001951"
     CDS             1598..1858
                     /locus_tag="VVN06_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563430.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
     gene            complement(2596..>2890)
                     /locus_tag="VVN06_001952"
     CDS             complement(2596..>2890)
                     /locus_tag="VVN06_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5563431.1"
                     /translation="IKKVGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKT
                     YNHALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT          911 a          455 c          547 g          977 t
ORIGIN      
        1 acatgaatgt caaaaagata atgtcaattt ttcaatcctt ttatgttgat gtcagtattg
       61 aggaactgac tttgacttta ccaatcagtt ttgtaaaaag gtttgagtat actcaaatga
      121 cttttcataa ggaatcattt ttattgatta aagaaaagag aagggggagt ttgagttcat
      181 ttgttactca ggctcgcact atgggtgaaa aagccaatat ggatgttgtt ttggtgtttt
      241 cgaagttatc agacagtgaa aaaaagcaat tacttcaagc tagagttccg tttgtagact
      301 ttaagggaaa cctcttcttc cctccattgg gactagtact gaatgcgaat gatactgaag
      361 tccctaagga attaacacct agcgaacaat taacgtggat tgccttttta ttgacaaaag
      421 gtcaaaaagt agtagatgtt gatttgcttt cacaagtcac tggacttcca aactcaacaa
      481 tttataggtg tttgaggact tttaaagctt tatattggtt aaacaagcaa aataagcttt
      541 acacatatac ggtgtcaaag aaagaattat tcttaaaatc cgtgtcatgt ttatttaatc
      601 ccatcaaaaa acggatttta ttgccagatg gcgatataaa gcagataaaa tctgtttcta
      661 accttctata tggtggtgct tatgctttgt cgcattcaac ttttttagct gaaacggatg
      721 aaaatactag ctatgtcata tggcagagaa aattcaatca gttatccttg ccactttctc
      781 agtatgtttt aaaatgaaag atgctagaga tatggaaata tcgtcctttt gtatctgagt
      841 tttggaatga ttttaaaaat aatcatgata aacaatttgt agatccgatt tctctttatt
      901 tgaccttaaa agatgatgat gacccacgta tagaggaaga gagtgaagca ctagaaaata
      961 tgatattaca gtatctggga gaagatgatg ccagctaata cgaaagttat ttttcaagaa
     1021 atgtttgcgg attttcagaa ctattatgtt ctgattgggg gaactgctac ctctatcgta
     1081 ttggattcgc aaggatttaa aagtcgcaca acaaaagatt atgatatggt catcattgat
     1141 gaagtaaaaa ataaggaatt ttatactacc ttgaatcatt ttttagaatt gggagagtat
     1201 caaggaagtc agaaagatga gaaagcgcag ctttttcgat ttacaacaac taatcttgag
     1261 tttccttcta tgattgaact atttagtatc ttaccagaat atccattaaa gaaggacggt
     1321 cgagaaattt ccttatattt tgaccaagat gctagtttat cagccttatt attggatgaa
     1381 gattattata atatattggt gcatgtaaaa gaaaccattc aggggtattc ggtattgagt
     1441 aattgtggtc tatactcttc gacaatctct tcaaaccacg tcagcttcca tctacaacct
     1501 caaaacagtg ttttgagcag cctgcggcta gcttcctagt ttgctctttg attttcattg
     1561 agtattaatt atttttaagg ctaaagcttg gctggatatg agggaacgct ctgctacagg
     1621 tgctcaaggt ttaagtaagt ccattaaaaa gcatttgaat gaccttaccc gtttgacagc
     1681 ttccttgcta ggagatgaaa agttatcggc tataacatca agtagtgcgg taaaagcaga
     1741 catgcaccgc tttgtgatag aattagagct tgtgaagtca actattcttc aaaatgatga
     1801 catttcatta gatcaaaatg aaatttttga aattctgaaa aattttttcg atggttaaaa
     1861 taattgtagc gagatggcta tattgaattc gtctatatct ggaaactaga aaaaacttca
     1921 atttcaggag aaaatgaagt caatcttccc acaatcaaac gtatagtatc aaggtttttc
     1981 aagacctgat attatgcgtt ttttgctttt caaaactttt tgcccagttt ttgtttttat
     2041 cctctagtca cttgatttgt ttcaggtggt tttttagtat agtaaaatga aacgagaaca
     2101 ggacaaattg atcaggacag tcaaatcgat ttctaacaat gttttagaag cagaagtgta
     2161 ctattctagt ttcaatctac tatagttaaa tctgcggtca agtctactgg tgaatctatg
     2221 attgtaatac tcttccaaaa tctcatcaac cacgtcagtc ttgccttgca gtctgtatct
     2281 tactgaccaa gctagtgatg gatttagaat aggtgatttg gagcgtccta ttggctagga
     2341 aatgctgctc atagtccttt gctgaggcta gggtgtttca acattcaaca ctcaactggt
     2401 tgatctagtt gataggaagg gagttactat aaaatactca ggcttccatc atattttttg
     2461 aaacgattgt gtaatcaaaa tgtaccaata ttgtagtatt ggtacagaag atgttgtgaa
     2521 tggataaata tatcataact gctatctcaa aaagatttca tatgtctgtg catatataat
     2581 agacttcctg caaaactaga atcctagttc atgattgata ataccagcaa tcaaattcat
     2641 tcgtaatcca aagcgtttac gatgatttcg ataggttgtt gaaaacattt taaacgtttc
     2701 tactttggca aagatgttct caaccttgct tctctcctta gatagcgcat ggttataggt
     2761 tttatcttca actgttagcg gcttgagttt gctggattta cgtggagttt gtgcttgagg
     2821 atatatcttc atgagccctt gataaccact gtcagccaag attttaccag cttgtccgac
     2881 ttttttgata
//
LOCUS       DAVHIU010000160         2855 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVHIU010000160 DAVHIU010000000
VERSION     DAVHIU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.162"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..151)
                     /gene="blpC"
                     /locus_tag="VVN06_001953"
     CDS             complement(<1..151)
                     /gene="blpC"
                     /locus_tag="VVN06_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU5563432.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPIQ"
     gene            complement(208..1569)
                     /locus_tag="VVN06_001954"
     CDS             complement(208..1569)
                     /locus_tag="VVN06_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU5563433.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDTIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            complement(1580..2838)
                     /locus_tag="VVN06_001955"
                     /pseudo
     CDS             complement(1580..2838)
                     /locus_tag="VVN06_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          799 a          480 c          579 g          997 t
ORIGIN      
        1 gttgtattgg atgatgaagt cctttcgtat tgttatgctt aattatatta aggctgtata
       61 agatatcttc ccacagtcct ccacctgtaa tttggttgag ttcggtagtt gttagttctt
      121 gaaatgaagt taggttttgt ttcttatcca tgttatgatt ctcctttttg ataagataat
      181 aaatagttat agagtgttat ctgaaaatta atcagaatgg gttaaaattt tatctttgaa
      241 ataatcaaaa tatgttttct ttgcagttac actagtgacg cgaccttgta agccatattg
      301 gatgagttta ctatcctcat tagatagttt tgcaagagcg gttaatttaa agagattacc
      361 ttgctctgtt ctggtaggag tttgatcaat tgtctgaagt tggccgatga tggtggtgcc
      421 gtgatttcca atcttctcca gttttaatct tacagtttgt cctttatcta gtagaggtag
      481 atagtcagaa gatacgtagt aagtgattag tacttctctt gtatctgtga tgacagggaa
      541 tatttgagca atttctgtac cagttggaat tctattttta ccttcaaatt cgctgttcag
      601 atgaacgata cctttacttg gagcggttaa ggtattgttt cctaagcgct gcgttgcttg
      661 atctagttgt acttttaatt ctgttaattg attctccacg gttagttgtt gctgtgaggc
      721 tgtttgtaaa aactgagtgc ggagtacttc aattttggtt gctaaactat tatcataagt
      781 tgctacactt ccgataccag cttgctgaat tttgaggctt gcgatagatg attccagacc
      841 tgcaatactt tgattaattt gagataaaaa tggctcctct gccgttcctt gtgtttgttc
      901 ttgagaggct ataagataac gatttaaaat tgactggtgc ggattgccag ttggtaagcg
      961 agctctttta tttacgatag tatctctcag ctcttgatat tctccaattt gttgttgaac
     1021 ttttgtaatt tcttgttcga tggctgatga actgctattg gtaagattag cttgatttga
     1081 aacttcagtg ttagtctttg agatacccag ttcaatatca tgggattgtt tagtaaaatt
     1141 cataaaagta ttatggtagc caaactcatc ctcgctagaa aaaagatcag tcgctttttc
     1201 taagctttgt ttcaagattc caagtccttc tttttgcttc tcaagtcttt gtaattgagt
     1261 ttctaaggca gttttctgac tctcttccat tgtttcagag tatttgatga gtaagtcacc
     1321 tttctcaact acttgatttg ccactaaatg attagcaagg ataggattat cactggtcga
     1381 ctgaatagat gcaatgacac ttgtaggggt gatttcccct tgggaagtaa cagtaatttc
     1441 ttttgtggca acaagggaga aaatcaagat gaaagtaaat aataatgaaa gaggtatgat
     1501 taatactgtc gcatagttat ggtaacgtct ctgataaaac tcgacgcttc taaaaagatt
     1561 aggattcatg acattctcct tatttattga acagatgatg gtagaagcct tgcgcctgca
     1621 ttaactcttg gtgactacca acttcaatga ttttcccctg gtcaagaaca atgacacggt
     1681 tagttcgttc ggctatactg agacgatggg ctacaaagag aatggtttta tcagttaggg
     1741 acataagatt atctataact tttttctcag tcaagacatc aagaccgcta gtagcttcgt
     1801 ctagtattaa aacaggagat ttagttaaaa gagcacgagc gagggctatt cgttgcttct
     1861 gtcctcctga tagaccagct ccatcagaga gctgagtttg atagcccata ggcattcttt
     1921 caatgtcttg acggatttca gctaattcac aagctcttag aatatcttct tggctaatca
     1981 tatgattacc gcccaaggtt aaattttcca agatagagcc attaaagata taggcttgtt
     2041 ggggtaggta attaatatga cggcgcaaga cttttttatc aatgttttta atatcctgat
     2101 gattgatgga aatatgccct ttgtagggtt caaagaaatt gacaatcatt ttggctaaag
     2161 ttgttttacc agaaccacta actccaacta ggctaacctt atctccttgt ttaatcgtga
     2221 gattaatatc tgttaaggta tctcgtccaa aaccatactt ataagaaagg tcatcaaatt
     2281 caatatcgcc catcaaaaaa tgtgaatgaa cagggttttc ttgaacttga aattcagatt
     2341 cgactagata gacttcgttc aaacggttat tagcgacctt cgcagattgg agtttggttg
     2401 gggttgatag ctgggtttgg gagctagaaa aatagctact ccagtccatt cagagaaaaa
     2461 gcgttctttt gacattttgg tgatttttac agaagggtca ggatcaccaa taaccagata
     2521 gtctttcttt gtttgataga caacatagta atgttggagt ttttcttctt tgttaacgtg
     2581 aacgataaat ggatagggga catcactcat gtcaaagaga gttttatctg cttgaacagg
     2641 tcttgtttca aagcccattt catcagcggc ttttacaatg ccaagagcag tcgttccttc
     2701 tttattggtc tttgcaagtt ctctcaagtg agctagagaa aaatctgaac catagaattt
     2761 agcaatcgag gctaaggcag caacaccgca gtctctcgca tctatttgag gaacaaatgt
     2821 acgtttataa gaagtcattg gcaattcctt tcata
//
LOCUS       DAVHIU010000161         2708 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVHIU010000161 DAVHIU010000000
VERSION     DAVHIU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.163"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(56..286)
                     /locus_tag="VVN06_001956"
     CDS             complement(56..286)
                     /locus_tag="VVN06_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU5563434.1"
                     /translation="MSKKDKKIEIQVADAKVNVSKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VVN06_001957"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VVN06_001957"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU5563435.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VVN06_001958"
     CDS             complement(1113..2036)
                     /locus_tag="VVN06_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU5563436.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2692)
                     /locus_tag="VVN06_001959"
     CDS             complement(2033..2692)
                     /locus_tag="VVN06_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU5563437.1"
                     /translation="MIVVGFILKFDTIATVVLAGLVTALVSGVSLVEFLEILGKEFSN
                     QRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFIRELAGFFSIR
                     LGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGNFFAQNTFVGA
                     GGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKLISKKTRGGEQ
                     K"
BASE COUNT          889 a          590 c          400 g          829 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacta acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaattgatt tagcatagtt aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caatcccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgcagccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggttg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaat
//
LOCUS       DAVHIU010000162         2633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVHIU010000162 DAVHIU010000000
VERSION     DAVHIU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.164"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..562
                     /locus_tag="VVN06_001960"
     CDS             <1..562
                     /locus_tag="VVN06_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU5563438.1"
                     /translation="MEVVDIIQSRYRELPFIDKFTLTPRNSIHMTVIELLCHENRETE
                     FWSSNLPLDTPLQEIHDYFAKQLEIFPLLDEEIHMRVTEMGKQNILVEPADEASAKRL
                     EEIRTYVSEKAGVRFPNHDRYQFHISIGYLRIPLTEEEEEEFTKVRAELTEILLEKIP
                     TITVNRIDYTVFEDMRQFVPYHEKFK"
     gene            587..1654
                     /locus_tag="VVN06_001961"
     CDS             587..1654
                     /locus_tag="VVN06_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5563439.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALTELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            1757..>2633
                     /locus_tag="VVN06_001962"
     CDS             1757..>2633
                     /locus_tag="VVN06_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563440.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGV"
BASE COUNT          927 a          349 c          527 g          830 t
ORIGIN      
        1 gatggaagtt gttgatatta ttcaatcacg ttatagagaa ttaccattta tcgataagtt
       61 tactttaact ccaaggaatt ctattcacat gactgtaatt gaattgttgt gccatgaaaa
      121 tcgtgaaacg gagttttgga gcagtaatct tcctcttgat acacctttac aggaaataca
      181 tgattacttt gctaaacaac ttgaaatttt tccattgttg gatgaagaaa ttcatatgcg
      241 tgtgactgaa atgggaaaac aaaacatact agttgaacct gcagatgaag cttctgcaaa
      301 gagattagaa gaaattcgta cttatgtttc agaaaaagca ggtgttcgtt tccctaatca
      361 tgatagatac caattccata tttcaattgg atatcttcgg attcctctaa ccgaagagga
      421 ggaagaagag tttactaaag tcagagcaga attaactgaa attttattag agaagattcc
      481 aactattact gtaaaccgta ttgactatac tgtatttgaa gatatgagac aatttgttcc
      541 atatcacgaa aaatttaaat aaaataataa aaaaggagaa ctgatcatga aaaaaatgaa
      601 agtttggtct actgtacttg caacgggagt tgctcttact acacttgctg cttgctctgg
      661 aggttcaaat tctacgactg cttcttcatc tgaagaaaaa gctgataaaa gtcaagaatt
      721 agtaatctat tcgaactcag tctcaaatgg tcgtggtgat tggttaactg ctaaagcaaa
      781 agaagctggt tttaatatca aaatggttga tatccctggc gctcaattag cagaccgtgt
      841 tattgctgag aagaataatg cagttgcaga tatggtattt ggaattggtg ctgttgattc
      901 aaataaaatt agagatcaaa aattactagt acagtacaag cctaaatggt tagataaaat
      961 tgatcaatct ttatcagata aagataatta ttataatcct gtgattgttc aaccattagt
     1021 tttaattggg gcgcctgatg taaaagaaat gcctaaagat tggacagaat taggtagtaa
     1081 gtataaaggt aaatattcaa tttctggtct tcaaggtggt acaggacgtg caattctagc
     1141 aagtatctta gttcgatacc ttgatgataa aggtgaatta ggtgtttccg aaaaaggttg
     1201 ggaagtagca aaagaatatt tgaaaaatgc atacactctt caaaagggag aaagttcaat
     1261 tgttaagatg ttagacaaag aagatccaat acaatatgga atgatgtggg gttctggtgc
     1321 attagttgga caaaaagaac aaaatgttgt tttcaaagtt atgactcctg agattggtgt
     1381 accatttgta actgaacaaa ctatggtttt aagcactagt aaaaaacaag cgttagctaa
     1441 agaatttatt gattggtttg gtcaatcaga aattcaagta gaatatagta agaactttgg
     1501 atctattcct gcaaataaag atgccctaac agagttacct gaagatacga aaaaatttgt
     1561 tgatcaagtg aaaccacaaa atattgactg ggaagctgtt ggaaaacatt tggatgaatg
     1621 ggtagaaaaa gctgaattag aatacgtaca ataagaaata agagttaatt tttatgtcca
     1681 agtctctcta ttatttctaa atttatagaa gaaataaaga gaggcttttg gtttagtaat
     1741 agagggagtt agtgagatga ttaaatttga taatattcaa attaaatatg gtgattttgt
     1801 tgcaattgat aatctgaatt tagatataca tgaaggggaa ttttttacat ttcttgggcc
     1861 ttcaggatgt ggtaaatcaa ctactttgag agcattggta ggttttctag atccatcatc
     1921 aggaagtatt gaagttaatg gaacagatgt cactcatttg gaacctgaaa agcgtggaat
     1981 tggtattgta tttcaatctt atgctctatt tccaactatg actgtttttg ataatattgc
     2041 atttggttta aaagttaaga aggtagctcc agatgttatt aaagctaaag tatcagcagt
     2101 agcagcaaaa attaagatct ctgatcaaca gttacagcgt aatgtatcag aattatctgg
     2161 gggtcaacaa caaagggtag cgttggctcg tgctctggtt cttgaaccta aaattctttg
     2221 tctagatgaa ccattgtcaa accttgacgc aaaattacgt gtagatttga gaaaagagtt
     2281 gaaaagactt caaaaagagt taggtattac tactttatat gttactcatg atcaagagga
     2341 agccttgact ctatctgata gaattgcagt ctttaacaat ggatacatcg aacaggtcgg
     2401 tacaccagta gagatttatc ataattctca aactgaattt gtatgtgatt ttattggaga
     2461 tattaatgtt ttgaccgatg aaacagtcca cgaagtatta ctgaaaaata caagcgtttt
     2521 cttagaagat aaaaaaggat acattcgatt agagaaagtt cgattcaatc gtgaaactga
     2581 acaagatttt attctaaaag ggacgattat tgatgttgag ttttctggag tta
//
LOCUS       DAVHIU010000163         2631 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVHIU010000163 DAVHIU010000000
VERSION     DAVHIU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.165"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            16..1023
                     /locus_tag="VVN06_001963"
     CDS             16..1023
                     /locus_tag="VVN06_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5563441.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1026..1226
                     /locus_tag="VVN06_001964"
     CDS             1026..1226
                     /locus_tag="VVN06_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU5563442.1"
                     /translation="MSEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1378..1560
                     /gene="rpmF"
                     /locus_tag="VVN06_001965"
     CDS             1378..1560
                     /gene="rpmF"
                     /locus_tag="VVN06_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5563443.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1576..1725
                     /gene="rpmG"
                     /locus_tag="VVN06_001966"
     CDS             1576..1725
                     /gene="rpmG"
                     /locus_tag="VVN06_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5563444.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2205..>2631)
                     /locus_tag="VVN06_001967"
     CDS             complement(2205..>2631)
                     /locus_tag="VVN06_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5563445.1"
                     /translation="MATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATG
                     WVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMA
                     TGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          863 a          527 c          470 g          771 t
ORIGIN      
        1 tgggaggtca ttcttatgac tgaaaaacta atcaattcaa aaccaaatgg tgtattcgca
       61 ttgattctca ttgagttgac aatcgtactt ggtatcttta tatttataat gggtgttggt
      121 tcggaaaaca tttttggaat tattatcgga cctttactaa tcgtaattgc aggtctagct
      181 catgctggtt taaaagttgt caaacctcag gaagctctgg ttctgacact ctttggtaat
      241 tatacaggta ccatcaaaga acctggtttt tactttgtca atcccttcag cgtagcagtc
      301 aaccctgcaa accacactcg acttggacaa agtggtgatg ttagcacaaa atctcctttt
      361 ttaggagcta aatcatcaaa tgacaatgat gtaaatcttg aaattggcaa gaaacagatt
      421 tccctcaaag tcatgacctt gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac
      481 cctgtagaaa tcggtatcgc ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc
      541 aacgttgata actacaaaga atatctttca ttgcaatgtg atagcgccct ccgtaatatt
      601 gtccgcatct atccttacga tgtgtctcct aatgtggata ctacgggtga cgggcaagca
      661 gatgaaggta gtctccgtgg ctctagcgaa attgttgcta accgtattcg tgaggaaatt
      721 caaagtcgcg tagaagatgc tggcttggaa atccttgaag cacgtatcac ttacctagct
      781 tatgctccag aaattgctgc cgttatgctt caacgccaac aagcatctgc cattattgat
      841 gcacggaaga tgattgtaga tggtgctgta ggaatggttg aaatggcact agaacggctc
      901 aatgaagggg aattagtaga acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc
      961 cttgtcgttc tttgtggcaa tcatgatgca caaccaattg tcaacacagg aagtctttac
     1021 taaagatgtc tgaagctaaa aaaaagcaga tcccccttcg actctcaaca aagttatacg
     1081 ctgcactcgc atcatgggca gaagacgact ttcgttcagt caatgggcaa atcgaatatc
     1141 tcctcacaga gtgtgtcaag caacggaaga aagacggaaa atacgtatca gaaaccattg
     1201 acgaaccatt tgagattgat atttaacacc ttctcctgtc tgccgtgtcg gataggagaa
     1261 ttttttcatc tttttttgtc aagtaacttt actttacaaa aaaaatgtgt tatcctaata
     1321 tggttgatga aaatcagtag attgaatcga atataaatac ctaaaggaga aatcaaaatg
     1381 gcagtacctg cacgtcgcac ttcaaaagcg aagaaaaaca aacgtcgtac acactacaaa
     1441 gtaacagctc catctgtaaa ctttgacgaa actactggag attactcacg ttctcaccgt
     1501 gtatcactta aaggatacta caaaggacgt aaaatcgcta aagctgcatc agctgaataa
     1561 tagaagggag ataccatgcg cgtaaatatt acacttgaac acaaagaatc tggtgaacgc
     1621 ttgtacctta cttctaaaaa caaacgtaac actccagacc gccttcaatt gaagaaatac
     1681 tcaccaaaac ttcgcaaaca cgttgtgttt acagaagtga agtaagcatt caatacgaat
     1741 caatacagaa gaaaaacgct tgatcagcgt tttttctttt tgttaatttc actatattgc
     1801 aagggaaatc attcgaatga ctacattttt tgaaataaga atacggattc ggacgattaa
     1861 aagtaagaga aactcaaatg taagttgggg ttcctttttt gtatattttt caaataacgc
     1921 ctctacacta tttgtagcaa attcaccaac tacagttgca tcttagttaa aataagttag
     1981 aatatgtaag tgagtaccag atataccaag acatcgtcac catctaaggt atattcaaaa
     2041 tacaaaagtt gaccaactag atttctgaat atccttatat atccattctt aaaattggtt
     2101 taaatagcgt agtcttttaa actagttttg agaatccaaa aaatcttcct acatatgtaa
     2161 gaagattttt tagttcagaa tgattagatt tagctaatgg atacctatcc tacccactca
     2221 ccgttagcat tgactcgata gccatcgggt gtagtcgtgt ttactaataa atctcctgaa
     2281 ttataggtat agtaccattt accagaaact gtaaaccaac cagtagccat tgaacctgat
     2341 tcatttaagt aataccataa gcctttgtct ttaacccaac cagtagccat tgaacctgat
     2401 tcgtttaagt aataccataa gcctttgtct ttaacccaac cagtagccat tgaacctgat
     2461 tcgtttaagt aataccataa gcctttgtct ttaacccaac cagtagccat tgaacctgat
     2521 tcatttaagt aataccataa acctttgtct ttaacccaac cagtagccat tgaacctgat
     2581 tcgtttaagt aataccataa acctttgtct ttaacccaac cagtagccat t
//
LOCUS       DAVHIU010000164         2615 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVHIU010000164 DAVHIU010000000
VERSION     DAVHIU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.166"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1023
                     /locus_tag="VVN06_001968"
     CDS             <1..1023
                     /locus_tag="VVN06_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5563446.1"
                     /translation="LVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWK
                     LVAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETM
                     GIDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVIAKRIIDIMGALVGLI
                     LCGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNT
                     MQGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYVQYTS
                     EQKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVF
                     MRDGAK"
     gene            1027..1770
                     /locus_tag="VVN06_001969"
     CDS             1027..1770
                     /locus_tag="VVN06_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5563447.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDIVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGKVLKAKRGVKY"
     gene            1767..2591
                     /locus_tag="VVN06_001970"
     CDS             1767..2591
                     /locus_tag="VVN06_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU5563448.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYRKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
BASE COUNT          866 a          344 c          577 g          828 t
ORIGIN      
        1 ttagtaaaga aatattggaa gcatgtgttt tttaatctaa aaaatagcaa gaaaatttta
       61 ctgttaacag taacgaaaaa tatggaaaaa gttcttgata aattgctaga atctgatgaa
      121 ctttcatgga aattggtagc agtaagtgtt ttggataaat ctgattttca acatgataaa
      181 atacctgtaa ttgaaaagga aaaaattatt gaatttgcaa cgcatgaagt tgtggatgag
      241 gtgtttgtca atcttccagg agagagctac gatattggag aaattatctc taggtttgag
      301 acaatgggga tagatgtaac tgtaaatctt aaagcatttg ataagaattt gggtcgcaat
      361 aaacaaattc atgagatggt aggattgaat gtagtcactt tctctacaaa tttttataaa
      421 actagtcatg taattgctaa gcggattatt gatatcatgg gtgcattggt agggctgata
      481 ctatgtggtt tagtcagtat tgtattggtt cctttgattc gaaaggatgg gggctctgct
      541 atttttgctc agacgcgtat aggaaaaaat ggtcgtcagt tcacttttta taagtttcgc
      601 tctatgtgtg tagatgccga ggcgaaaaaa agagaactca tggaacaaaa taccatgcag
      661 ggtggaatgt ttaaggtgga cgatgatcct cgtatcacga aaattggtcg ttttatacgg
      721 aagactagct tggacgagct accacagttt tataatgttc taaagggaga tatgagtttg
      781 gttggcacac ggccacctac agtggacgag tatgttcagt atacttcaga acagaaacgt
      841 cgactcagct ttaaacctgg tattacaggt ttatggcagg ttagcggccg tagtaaaata
      901 accgattttg acgatgttgt aaaattagat gtggcttata ttgataattg gacaatctgg
      961 aaagatattg aaattttgct taaaactgtt aaagttgtat ttatgagaga tggagcgaag
     1021 tgaggaatga gggatagaat ccaactttta ggtgtaacaa ttgatttgct tacgatgaat
     1081 gaaacgatag atattgtaga acaatatgta ttagaaaaaa gaccactaca cttgatgggg
     1141 gtgaatgctg ataaaattaa tcagtgtcat acagatgaga aaatcaaaaa aatcgttaat
     1201 gagtcaggaa tcattaatgc ggatggagca tcagttgttc ttgcaagtaa gtttttagga
     1261 acgcctgttc ctgaacgagt agcgggtatt gatttgatgc aatgtctttt agagttgtca
     1321 aataaaaaag gatattcagt ttactttttt ggagcaaaag aagaagtttt gcaagatatg
     1381 ctcaaagtat ttaagagaga ttatccaaat ttgatagtta ttggacacag aaatggctat
     1441 ttttctgaag aggatgaaca agctattcaa gaagatattc gtgaaaagaa ccctgatttt
     1501 gtgtttattg gaattacgtc tcctaaaaaa gaatatatta ttcaaaaatt tatggatagt
     1561 ggcgtcaatt cggtatttat gggagttggc ggtagttttg atgtcttgtc tggtcatatc
     1621 caacgagcac ctctatggat gcaaaagtca aatttagagt ggttattccg tgtagctaat
     1681 gagcctaaac gtctctttaa acgttatttt gtagggaata tttcattcat aggaaaagtt
     1741 ttaaaagcaa aaagaggtgt aaaatattga accagacaga gatgattcgc ttaattcaaa
     1801 aagttgaatt agatgctata aaagagttta aaaaaatctg tgaagagaat gatatagatt
     1861 ttttcctccg cggtggtagt gtacttggtg cggtcaaata cgacggcttt attccatggg
     1921 atgatgatat ggatatcgct gtccctcgtg aagcatacga caaacttcca agtgttttca
     1981 aagatagaat tatcgctggg aaatatcagg ttcttactta tcaatactgt gatacgttgc
     2041 attgctactt tcctcgacta ttccttttag aagatgaaag aaaacgtttg ggcttgccac
     2101 gaaataccaa tctaggattg catttgattg atatcattcc tttagatgga gcaccaaatc
     2161 attcggtttt aagaaagatt tactttggca aagtatactg gtatcgtttt ttagcaagtt
     2221 taggaacaac ttatgttggc gaccatgtgg atatgcattc cactaagcaa aaactaatta
     2281 ttggtttctt taaaaaacta ggatttgcaa aactatttcc tcaaaattct gtatacagac
     2341 gcttggataa tctctataga aagtatgatt ggaaaaagca gaagtatgcg gggactatca
     2401 atgcttcttt atttgctaaa gaagttatgc cagtagagat ttggggagaa ggagtagaga
     2461 agccttttga ggataccttc tttaaagttc caacggagta tgatcgctac ctgaaaagac
     2521 tttacggaga aaactatctt tacgaagagc ctagtgatga tgaaaagaaa tcgcatttag
     2581 gaggacacta atttgttttg ttatattatt ttgca
//
LOCUS       DAVHIU010000165         2554 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVHIU010000165 DAVHIU010000000
VERSION     DAVHIU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.167"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            132..>2554
                     /locus_tag="VVN06_001971"
     CDS             132..>2554
                     /locus_tag="VVN06_001971"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5563449.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRVSFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNR"
BASE COUNT          762 a          437 c          571 g          784 t
ORIGIN      
        1 atataaaaat gatgacaaaa atacaaatga atagaaaata aattagtaag ctgatgaaat
       61 ttttctcaag agaagccatt tataggtgaa aatggtataa tatagtgaga aggatagagg
      121 agaagtgtaa attgatcgca caactagata caaaaacagt ctatagtttt atggaaagcg
      181 tcatttcgat cgaaaagtat gtgagagcag ctaaagaata cggctacact catttggcta
      241 tgatggatat tgacaatctt tatggcgctt tcgactttct agagattaca aaaaaatacg
      301 gcattcatcc tttgctaggg cttgaaatga cagtgtttgt agatgatcag gaagtgaatt
      361 tgcgcttttt agctctatct agtgtgggct atcagcagtt gatgaagctt tcgacagcca
      421 agatgcaggg ggagaaaact tggtcagtcc tgtcccagta cctggaggat atcgcggtca
      481 ttgtgcctta ttttgataga gttgagtcgt tagaactagg ctgtgattac tatatagggg
      541 tttatccaga aacactagca agcgaatttc atcatcctat cttacctctt tatcgggtca
      601 acgcttttga aagcagggat agagaagttc ttcaagtttt aacagcgatt aaagaaaatc
      661 taccgctcag agaagttccc ttgcgttcga gacaagatgt ctttatatca gcaagttctt
      721 tagagaaact attccaagag cgttttccgc aagctttgga caatttagaa aagcttattt
      781 caggcatttc ttacgacttg gatactagtc tgaaactgcc tcgttttaat ccagctagac
      841 cagcagtaga ggagttgaga gagcgtgctg aactggggct tgttcagaag gggttgacta
      901 gtaaagaata tcaagataga ctagaccaag aattgtctgt tattcatgat atgggctttg
      961 atgattattt cttggttgtt tgggatttgt tgcgttttgg acgatcgaat ggctattata
     1021 tgggaatggg aaggggttct gcagtaggca gtttggtttc ttatgcctta gacatcacgg
     1081 ggattgaccc agtagagaaa aatctgattt ttgaacgctt tcttaatcgt gaacgctata
     1141 ccatgcctga tattgatatt gatatcccag atatttatcg tccagatttt atcagatatg
     1201 ttggtaataa atatggtagt aaacatgcgg cacaaatcgt tactttttca acctttggag
     1261 ccaagcaagc tcttcgagat gtcttgaaac gctttggtgt gccagagtat gaattatctg
     1321 caattactaa gaaaatcagt tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc
     1381 agtttcgtca gcaaatcaat agtaagttag aataccaaaa agcttttgag attgcttgca
     1441 agatagaggg ctatccaaga caaacctctg tccatgcggc tggtgttgta attagtgacc
     1501 aagatttaac caactatatt cctctaaagt atggtgatga aattccactg actcagtatg
     1561 atgctcatgg agttgaggct agcggacttt tgaagatgga ctttctggga ctacgaaatt
     1621 tgacctttgt ccagaagatg caagagttgc ttgctgaaat agaaggtatt caccttaaaa
     1681 ttgaagaaat agatttggaa gacaaagaaa cgttagattt atttgcctct ggaaatacaa
     1741 aaggtatctt tcaatttgag caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag
     1801 tctgttttga agatgtcgtc gcgactactt ctctaaatcg accgggtgct agtgactata
     1861 tcaataattt tgtggcaaga aagcatgggc aggaagaagt gactgttctg gatccagtac
     1921 tggaggatat tttggctcca acctacggca taatgctcta tcaggagcag gttatgcagg
     1981 ttgcccagcg atttgccgga tttagtcttg ggaaagccga tattttgcgt cgggctatgg
     2041 ggaaaaagga tgcctctgcc atgcatgaga tgagggtttc ctttattcaa ggttcattag
     2101 aagctggtca tactgtggaa aaagcagagc aggtctttga tgttatggag aagtttgcag
     2161 gttatggttt taacaggtca cacgcctatg cctactcagc cttggccttc cagttggctt
     2221 attttaaaac acattatcca gccatttttt atcaggtcat gttaaattct tccaacagtg
     2281 attacttaat agatgcactt gaagcaggtt ttgaagtagc ctctctatcc atcaacacca
     2341 ttccctatca cgataaaatt gccaacaagg ccatctatct aggtttgaaa tccattaaag
     2401 gagtcagtaa tgatttagct ctctggatta ttgaaaatag accttattct aacattgaag
     2461 attttatagc taaattacct gagaattatc tgaaacttcc tctgctagaa cctttggtaa
     2521 aagttggtct tttcgattca tttgaaaaaa atcg
//
LOCUS       DAVHIU010000166         2514 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVHIU010000166 DAVHIU010000000
VERSION     DAVHIU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.168"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1127)
                     /locus_tag="VVN06_001972"
     CDS             complement(<1..1127)
                     /locus_tag="VVN06_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563450.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYN"
     gene            complement(1137..1553)
                     /locus_tag="VVN06_001973"
     CDS             complement(1137..1553)
                     /locus_tag="VVN06_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin"
                     /protein_id="HEU5563451.1"
                     /translation="MIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLG
                     LVSGICELLVQSHHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLE
                     GGNKDQKVRVRIYGKNTDVQNIELVFNSEKIIELNF"
BASE COUNT          772 a          540 c          341 g          861 t
ORIGIN      
        1 ttattatata aaggcattgt ttcatcattc gaataaagtc cttgataaaa tccgtttaat
       61 gaactaatcc taccttctcc attccaaaat acagcttttg ttctatttac aacttcttcc
      121 ttacttggag ctggattttg tatagcatgt ctaaagaaag gaataatacc tttagtaaat
      181 gctggagttg gtacatcatt tgtcataaat gtatacgcgg cacattcgaa attataaact
      241 gtgcctcccc cagtatatac attcatcatt tccatagcaa tcattgattc tggttccgat
      301 gcataggatc tcatatccct agctcttcca gtttcaaatg tgtttgtata atgtttttcc
      361 caccatttcc atgtatctgt tgatgagccc cagttatcac ataagccact caaccaaaat
      421 ccactaacga tagaatctgt acccgcatca tctcttattg gcgtattttt agttgccaac
      481 accaaatttt tatgatattt ttgactcgct tcaaagaatg tcggatcatt cataatagtt
      541 tcccagaacc atttttcatg atcatgccag ataaaatgcg ctccatattt ggcacaaact
      601 tccaaatatt tagcactatg cggagctaac tggttattgt aaatccaata attctcaata
      661 tttaaaacac cttttaacac actatacttt tggaattgtt catctaacca ctctggagga
      721 actgtattac gctctccagc cgacataata accaagaaaa ctggaatgtt tagactttga
      781 gcttcctcca acatcttgac ataccattct ctcaattctt tcgtatctcg tggaatacaa
      841 cttgttggtt tacagacttt tgcaggatga agttcaatcg ctgcatatgg ttttacatct
      901 tcaggaatag cttcccatgc tcccttcaat gtgttccccc accataatcc gttaccatta
      961 tcattgccat acaacggaga aagcaacaat ggcgattcat tattaatcgt cgttctcatt
     1021 tgaacacgat tatcagcctt aactcctgct aacaatccaa aacataacat cccaatacat
     1081 acaaacagta ttgtgattaa ttttcttttt attttttctt tattcatgac ttatacctaa
     1141 aaatttaact caataatttt ttctgaatta aataccaatt caatattttg tacatcagta
     1201 tttttgccat atattcttac tcttactttt tgatctttgt ttcctccttc caattttagg
     1261 aatgttatat ccccgttttt ccaagcaaac gataccttat atcctcctct tactctgaaa
     1321 cctttcactt ctccttctga ccaagcagaa ggtaaagctg gaattagtga taaccaatta
     1381 tgatggctct gtactaataa ttcacaaatt ccactcacca aacctaaatt accatcaatt
     1441 tgaaatggtg gatggtcaag aaataaattg ccaagagtcg cattatttaa caaaccatta
     1501 atctggttat aagcaggttc accttgatat agtctcgcaa aaaaatgaat cagccatgca
     1561 gcactccaac ctgtttgtgt actagcatgc aaaccactta ctaaccaatt attaatcgct
     1621 tgctccctct cctgtgaaga taaaaagtta gcgtttgata atctcctatt gatagtgatt
     1681 ttagctgctt ctgctaattc cggagtttta tgaatatcaa tctcattata aggataaagc
     1741 ccaaatagag gtgaaatgtg tctatgccca ggctctactt cttcataatc ttctaaccat
     1801 tcttggattt gcccattact acctattttt gttttaggta gtttcttttt taactccttc
     1861 acacgactaa taaaatccga attgtctcct aattgttttg caatgccaat gcatgaatca
     1921 caaaaatatc ttagaatttg attatcaatt gtagatgata gacaagcatt tccttcaata
     1981 ccatttttta agcgatattt attttctggt gagacacttg gacctgtcat caagtagcca
     2041 tccacctcaa ataaataatc ttcaaagaaa agaaatgctt cttttatcat ttcaaaatgt
     2101 tccgtaagaa tacgctcatc ttggaaatat aaatagtgtt cccaaatatg agtacataac
     2161 catggaatag ttaataccca aattgcagcc cccatggcat gagattgggg agccgtatcg
     2221 ccaaaaccat ccgtattatg atgtgctgta aaacctctag ctccatacat tttcttagcg
     2281 gttagtcttc ccggttctct cattctttcg agcatatcaa ataatggata ttctacttct
     2341 ggtaaatcac atggacctac catccaataa ttcatttgag tattaatatt aatcgtatat
     2401 ttagaacccc aaattggatt taattcatca caccatattc cttgaagatt ggcaggtaaa
     2461 ccattcggtt gactagacga tattaacaga tatcttccat aatgaaataa caag
//
LOCUS       DAVHIU010000167         2498 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVHIU010000167 DAVHIU010000000
VERSION     DAVHIU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.169"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            81..1772
                     /locus_tag="VVN06_001974"
     CDS             81..1772
                     /locus_tag="VVN06_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5563452.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKARFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            1775..2485
                     /locus_tag="VVN06_001975"
     CDS             1775..2485
                     /locus_tag="VVN06_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5563453.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT          741 a          385 c          468 g          904 t
ORIGIN      
        1 gctgctatta gatctgtcgg agaaagtgtg gaattattta tcacaccatc agacgttctg
       61 caattttaag gaggtgcagt atgcgtcata aattaaattt aaaagattgg cttattcgtt
      121 tagggttaat ctggttctta gtaacattta ttatttatcc aaactttgat ctagtagtga
      181 atgtatttgt aaaaggagga gaattttccc ttgatgctgt acatcgtgtt ctaaaatctc
      241 agagggcact tcagagtatt atgaacagtt ttaagttagc attttcactc attattacag
      301 ttaatgtcgt aggtattctt tgtgttctat ttacagagta ctttgatatt aaaggtgcta
      361 aaattttaaa attaggttat atgacctctt taatttatgg aggagtggtt ttagcgactg
      421 gatataaatt tgtctatggt ccttatggat tgataacaaa atttttacaa aatgttatcc
      481 cttctttaga ccctaactgg tttattgggt atggtgcagt cttattcatt atgacatttt
      541 caggaactgc taatcataca ttgtttttaa caaatacaat tcgaagcgtt gactatcaca
      601 ctattgaggc tgctcgaaat atgggagcaa aaccatttac tgttttccga aaagtagtgt
      661 taccaacctt aattccaact ctatttgcac ttactattat ggtttttctt agtggtttat
      721 ctgcagtagc agcacccatg attgttggtg gtaaagaatt tcaaactata aatccaatga
      781 ttattacatt tgcagggatg gggaattctc gtgatttagc tgccctactt gcaattattt
      841 taggtattgc aactacaatt ttgcttacta tcatgaataa gatagaaaaa ggtggaaatt
      901 atatttctat ctctaagact aaagcgcctc ttaaaaaaca aaaaattgcg tctaagcctt
      961 ggaatatcat tgctcacatt gtagcatatg gattgttcac agttttcatg cttccactaa
     1021 tttttatagt attatactca tttacagatc cagttgcaat tcaaacaggt aacttaacat
     1081 tatcaaactt tactttagaa aattatcgcc tattctttag taatagtgcg gcattctctc
     1141 cattcttggt cagctttatt tattctatta tcgctgcgac aacagcaaca attctcgcag
     1201 ttgtatttgc tcgagttgtc agaaaacata aagctcgttt tgatttttta tttgaatatg
     1261 gtgctctact tccttggtta ctaccaagta cacttttagc agtaagttta ttatttactt
     1321 ttaatcagcc acaatttctt gtcttgaatc agattttggt aggtagtttg gtaattctac
     1381 ttattgcata tatagttgta aaaatcccat tttcttatag aatggtacgt gctattttat
     1441 ttagtgttga tgatgagatg gaagatgcag caagaagtat gggtgcttca cctttttata
     1501 ctatgatgaa ggttatcatt ccatttattt taccggttgt tctctctgtt attgctttaa
     1561 actttaactc tttattaact gacttcgact tatctgtatt cctttaccat cccctagctc
     1621 aaccattagg tattacgatt cgatctgcag gtgatgaaac agcaacatct aatgcacaag
     1681 ctctggtatt tgtttataca attgttctga tgatcatttc tggaacggta ttatacttca
     1741 cacaaagacc ggggcgtaaa gtaaggaaat aatcatgaca gccactagtc ttgggttatc
     1801 aaatattgaa atagttgtca ggattgtttt atcagtagtc attggtagta taattggttt
     1861 agagagaggg agcaaatccc agcctgcagg catccgaact tatagtattg tttgtctagc
     1921 tgcatgtttg attatgatga cgaatgaata cgtatcttat aaatttggga caggagatcc
     1981 tacacgatta ggagctcaag ttatatcagg tgtgggtttt ctaggcgctg gaacgattct
     2041 tattacagat aaaaagaaaa ttacaggtct gacaactgca gcaggcattt gggcttcggc
     2101 aggaattgga ttagctattg gagtaggttt ttatgaggga gctcttttag tagccatttc
     2161 tgtttggggt gtgatatcca tgttccaacc actaaaaaaa tatctgcaaa atcgttctaa
     2221 aatgattgaa ttgtatatag tggttaaatc tacagaagcc tataatcgtg tattggttta
     2281 ttgtgcagaa aatggtatca gaatgaccga ttcaagaaca gcgtttggag atgttaattc
     2341 tgacagaatt gaatactttg atgttccaga taaaaaaata gcgtcattta ttactctgga
     2401 attatcaggt agatttgaac atcttcgact gatggaagaa attgctaata ttgtaggtgt
     2461 tatttatgtc gaagaaatta gctgaaattt agtaaaca
//
LOCUS       DAVHIU010000168         2460 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVHIU010000168 DAVHIU010000000
VERSION     DAVHIU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.170"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..184
                     /locus_tag="VVN06_001976"
     CDS             <1..184
                     /locus_tag="VVN06_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEU5563454.1"
                     /translation="LVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVC
                     YPISKLSTHLEKHWRN"
     gene            185..942
                     /locus_tag="VVN06_001977"
                     /pseudo
     CDS             185..942
                     /locus_tag="VVN06_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            954..1819
                     /locus_tag="VVN06_001978"
                     /pseudo
     CDS             954..1819
                     /locus_tag="VVN06_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(1899..2444)
                     /locus_tag="VVN06_001979"
     CDS             complement(1899..2444)
                     /locus_tag="VVN06_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563455.1"
                     /translation="MTGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIPANL
                     GALIEEPGFINYYSGFKNLQYLASIRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGM
                     RKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHSEED
                     IRILCDKVYAIEDKVCTLCSD"
BASE COUNT          763 a          540 c          436 g          721 t
ORIGIN      
        1 attagttgtt ttgattgggg ttgtggaagt gaccaaagtt ggacaacaaa tcatcgatag
       61 caatcgcctg accatcccaa ctgcttcatt ttggatttat ggaaccattc tagtcttata
      121 tttcgcagtt tgctacccta tttccaaact atccactcac ttagaaaaac attggagaaa
      181 ctaaatgtct gaaactatct tagaaatcaa ggaactaaaa aaatccttcg gagacaatcc
      241 catcctccaa ggactttctc tagaaatcaa aaaagggaag ttgttgtcat cctagggccc
      301 tctggttgtg ggaaaagtac cctccttcgt tgcctcaacg gcttagaaag tattcaaggt
      361 ggagatattc ttctggatgg tcagtctatc gttgaaaata aaaaagattt tcacctagtt
      421 cgccaaaaga ttggcatggt ctttcaaagt tatgaactct ttccccatct ggatgtctta
      481 caaaacctca tcctaggccc tatcaaagct caaggaaggg acaagaaaga agtaacggaa
      541 gaagctttgc aattactaga tcgtgtcggt ttgctggata aacaacatag ctttgcccgt
      601 caattatctg gtggacagaa gcaacgtgtt gcaattgtcc gtgccctcct aatgcatcca
      661 gaaatcatcc tttttgacga ggtgactgct tcgctggatc cagaaatggt gcgtgaggtg
      721 ctggaactta tcaatgattt ggcccaagaa ggccgtacca tgattttagt aacccacgaa
      781 atgcagtttg cccaagccat tgctgaccag attatcttcc tcgaccaagg gaaaatcgct
      841 gaagaaggaa cagctcaagc cttctttacc aatccgcaaa ccaaacgagc ccaggaattt
      901 ttaaacgtct ttgactttag ccaattcggc tcatatctat aaaggagatt cttatgaaac
      961 tattcaaacc actcttaact gttttagcac ttgcctttgc ccttatcttt atcactgctt
     1021 gtagctcagg tggaaacgct ggttcatcct ctggaaaaac aactgccaaa gctcgcacta
     1081 tcgatgaaat caaaaaaagc ggtgaactgc gaatcgccgt gtttggagat aaaaaaccgt
     1141 ttggctacgt tgacaatgat ggttcttacc aaggctacgc tacgatattg aactagggaa
     1201 ccaactagct caagaccttg gtgtcaaggt taaatacatt tcagtcgatg ctgacaaccg
     1261 tgcggaatac ttgatttcaa acaaggtaga tattactctt gctaacttta cagtaactga
     1321 cgaacgtaag aaacaagttg attttgccct tccatatatg aaagtttctc tgggtgtcgt
     1381 atcacctaag actggtctca ttacagacgt caaacaactt gaaggtaaaa ccttaattgt
     1441 cacaaaagga acgactgctg agacttattt tgaaaagaat catccagaaa tcaaactcca
     1501 aaaatacgac caatacagtg actcttacca agctcttctt gacggacgtg gagatgcctt
     1561 ttcaactgac aatacggaag ttctagcttg ggcgcttgaa aataaaggat ttgaagtagg
     1621 aattacttcc ctcggtgatc ccgataccat tgcggcagca gttcaaaaag gcaaccaaga
     1681 attgctagac ttcatcaata aagatattga aaaattaggc aaggaaaact tcttccacaa
     1741 ggcctatgaa aaaacacttc acccaaccta cggtgacgct gctaaagcag atgacctggt
     1801 tgttgaaggt ggccattaac tcttaaaagg gactggattt taagctccaa tcccttttta
     1861 agattttacc tataacatcc tgagtctatc taagatgttc aatctgaaca cagtgtacat
     1921 actttatctt ctattgcata tactttatca cataagatac gaatatcctc ttcactgtga
     1981 ctagcaatca aaattgttgt ccctttttca ctagagagct ttctaaacaa tgttctcata
     2041 ttttctacac ttgatttatc caaggcattc ataggttcat ctagtaaaag aatagaggga
     2101 ttctccataa ttgcttgagc aatccctagc tttttcctca tacctagcga ataagtttta
     2161 actttctggt ctttttgctc atatagacca actattttca gtgtatcatt gatttcctga
     2221 ttaccaacta ctcctcgtat gcttgccaaa tattgtaaat tcttaaagcc actataataa
     2281 tttataaaac caggttcttc aatcaaagct cccaaattag ctggaatttt tctctcagga
     2341 acaatatttt ccccattgat taacacttct ccataagacg gactatataa accagctatt
     2401 aatttaaaca atacactttt ccctgagcca ttcgcaccag taattcctat aatttccccc
//
LOCUS       DAVHIU010000169         2233 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVHIU010000169 DAVHIU010000000
VERSION     DAVHIU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.171"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..243
                     /locus_tag="VVN06_001980"
     CDS             <1..243
                     /locus_tag="VVN06_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5563456.1"
                     /translation="QKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN
                     EFADFVVQIDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            303..539
                     /locus_tag="VVN06_001981"
     CDS             303..539
                     /locus_tag="VVN06_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU5563457.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            536..949
                     /locus_tag="VVN06_001982"
     CDS             536..949
                     /locus_tag="VVN06_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU5563458.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1067..2032
                     /locus_tag="VVN06_001983"
     CDS             1067..2032
                     /locus_tag="VVN06_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5563459.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
BASE COUNT          821 a          331 c          437 g          644 t
ORIGIN      
        1 cagaaattta ttatccatgt tttaaggaat aataattata gtcgagtgat atcaggaagt
       61 gctcagcctc agttaccaat tacaaaatta aaaaaaatac ttctccccct ccccccacta
      121 gccctccaaa atgagttcgc agactttgta gttcagatcg acaaatcaca attggcaatc
      181 caaaaatctc tggaagaact tgaaactttg aagaaatctc tgatgcagga gtattttggc
      241 tgatattctg ccattgtaat tacggtaatg atttgttata atacttcaaa ggaggaaatc
      301 agatggtagt aaaaacaaga aaacaaggaa attcaatcac cattacgatt ccaagtgaat
      361 ttaatattcc aagtggtgtt aaatacgaag cgaaattgtt accaagtggt gagattatct
      421 ttactcctga agaattgggg cagcaggttt cttatgtatc tgatgatgcc tttgacttaa
      481 atttagataa aatatttgac gaatacgacg atgttttcaa agctttggtg gaaaaatgac
      541 aatctatctg acagaaaagc aaattgaaaa aataaatgct ttagcaattc aacggtattc
      601 tccaaatgag aaaattcaaa cagttagttc ttctgcctta aatatgattg tgaacttacc
      661 agaacaattt gtctttggga agtctcttta tccaacaatt tttgataaag caacgatact
      721 atttgtccaa ttgataaaga agcatgtttt tgctaatgct aataaaagaa ctgctttctt
      781 cgttttggtc aaatttttac aattaaacgg ctatcgtttt tctgtaacgg tagaagaagc
      841 agtaaaaatg tgtgtaacca tcgcagtaga agctttaact gatgaaaaaa tgacaagcta
      901 ctccaaatgg gtttctgaac attctgttag agaaaaggtc aaaaagtaac ctagtatgct
      961 ggatttgaat gagcacaaga aaataaatga acagacaata ttagaattct gtaatgcaga
     1021 aactgatatt gtctcttttt attgatgaat aagaaagtga gaaattatgg aatcaaaagt
     1081 tacaattatc atgcaagaaa tgttacctct tttaaataat gaacaattac tagcgttgag
     1141 agagagttta gaacatcatc tagtagacgg aaaaaagcag cagaagtatt cgaataataa
     1201 cctgttgcaa ctatttatta ccgccaagca ggtagagggc tgtagctcaa aaacaattcg
     1261 ttattaccag aggacgattg aaaacttgtt taatgctatt aaagagtctg tgacacaact
     1321 cacaacagat gatttaagga gttatttagc aaattaccag tctgaaaagg attgtagtaa
     1381 ggcaaattta gacaatatta ggcgtatatt gtcttctttt tttgcttggc ttgagcaaga
     1441 ggaatatatc attaaaaatc ccattcgacg gataaagaaa attaagactg agcaaaatgt
     1501 gaaggaaact tatactgatg aacatttgga aattatgcgt gataactgtg aaaatttgag
     1561 agatttggca ataatagacc tactagcatc gacaggtatg cgtgtagggg agcttgtaca
     1621 gttgaatcgt tcagatattg attttgaaaa cagagagtgt gttgtctttg gtaaaggaaa
     1681 gaaggagaga ccagtatatt ttgatgctcg gacgaaaatt catttaagaa attatcttaa
     1741 cgacagaaaa gatagtcacc ctgctctttt tgtaacgcta gttggaaaag tccagagact
     1801 tggaattgct ggtgtagaga ttcgcttaag aaagttagga gacaaactcg gcatacaaaa
     1861 ggttcaccca cataagttca gaagaacttt agcgactaag gcaattgata aaggtatgcc
     1921 tatcgaacaa gtccaaaaac tgctaggtca tagcaagatt gacacaaccc tggcctatgc
     1981 catggtcaat caaaataatg tcaagcattc acaccaaaaa ttcatctctt aaaagcaaat
     2041 cccgatttaa cgagatgttt ggggatgtta ttctaaatga aaaagaatgg aaagttagca
     2101 aatggaacga gattctaaca ataagaaatg gtaaaaatca aaaacaggtt gaagatgctg
     2161 atgggaaatt tcctatttat ggaagtgggg gaattatggg atatgctaaa gattggatag
     2221 tgaagaaaaa ttc
//
LOCUS       DAVHIU010000170         2226 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVHIU010000170 DAVHIU010000000
VERSION     DAVHIU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.172"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1113
                     /locus_tag="VVN06_001984"
     CDS             <1..1113
                     /locus_tag="VVN06_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU5563460.1"
                     /translation="SAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATN
                     YPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIFLNSQH
                     VPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKF
                     ESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAM
                     SEDIPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIE
                     VMGLAILPPRLKTELEEVGKYLLGEDNAIAAYHLEWADQLKEKYSGINKEEVNSVVQH
                     EAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1126..2136
                     /gene="galE"
                     /locus_tag="VVN06_001985"
     CDS             1126..2136
                     /gene="galE"
                     /locus_tag="VVN06_001985"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5563461.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVVIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
BASE COUNT          761 a          320 c          469 g          676 t
ORIGIN      
        1 tctgctattg ctaagaatat tgagtttcga gcatcaacta aatacggaga gctagagatt
       61 acgataaatt tgtcaaaacc cgaaaaagat ccaaaaacaa ttgctgctga aaaattagta
      121 aaagcaacta attatcccaa atgtttacta tgtatggaaa atgaaggata tcaggggcga
      181 ataaattatc cagcacgttc gaatcataga attattcggt tgaaacttgg tgacgaagta
      241 tggggattcc agtattctcc ctactcatac tttaatgagc atgcgatatt tttgaatagt
      301 caacatgtac caatggctat tacctctaaa acatttgaac aattattgga gattgttgat
      361 attttgccag gatattttgc tggttcaaat tctgatcttc ctatctcagg aggctccatt
      421 cttagtcata accactacca gggagggaaa catattttcc caatggaaaa agcaaaattt
      481 gagagtgaat ttcgttttaa agactttgag gatgttaatg ctggtattgt aaaatggcca
      541 atgtcagtaa ttaggttaca aagtgaaaat aaaaatcgct tgttagattt agctactaaa
      601 attctaaata agtggagaga atattcagat ttagaagtag acatcattgc tatgtctgaa
      661 gatattccac accatacggt gactcctatt gctcgtaaag tagatgggag atatgaattg
      721 gatattgttc tgagagataa tcatactact gaacaatacc cagatggtgt tttccatcct
      781 catcaagatg tacaacatat taaaaaggaa aatattgggt taatcgaagt gatgggactt
      841 gcgatattac cacctcgttt gaaaacagag ttagaagaag ttggaaaata tcttttagga
      901 gaagataatg caattgcggc ttatcattta gaatgggccg accaattgaa agaaaaatat
      961 tctggtatta acaaagaaga ggttaatagc gtagttcaac acgaagcagg acaagtgttt
     1021 gcgagagttc ttgaagatgc aggtgtttat aagaatactc cgtctggaca tgaagcgttt
     1081 atgagatttg ttaaatcagt tggaattaat taggaggaaa cccaaatggc aatattggta
     1141 acaggcggtg ctggttatat tggtagccat accgtagtag aattactaaa tttaggaaag
     1201 gaagtcgtca ttgttgacaa cctttctaac tctagcatct tggtgttaga ccgtattgaa
     1261 gcaattacag gaatacgtcc cgtgttttac gaattagatg tttgtgataa acaagcattg
     1321 agaaaggtat ttgaacaaga atcgattgat gctgcaattc attttgcagg ttataaagct
     1381 gtcggagaat ccgtgcaaaa gcctgtgatg tactacaaaa ataatattat gagtacattg
     1441 gcacttgttg aagtgatgtc agaatttaat gttaaaaaga ttgtcttttc ttcaagtgcg
     1501 actgtatatg gaattaacaa tcagtcacct ctaattgaga cgatgcaaac aagtgcgaca
     1561 aatccttatg ggtatacgaa agtgatgctt gagcaaattt taaaagatgt tcatgtggca
     1621 gattcagaat ggagtattgc gttgcttcgt tatttcaatc caattggtgc tcatgagtct
     1681 ggtttgattg gggaagatcc ctcaggaatt cctaacaact tgatgcccta tattgcgcaa
     1741 gtagcggtag gtaagctgtc agagctaagt gtgttcggaa atgattatga tacgcttgat
     1801 ggtactggtg tgcgtgatta tatccatgta gtagatttag caataggaca tataaaagct
     1861 ttagaaaaag tatctgaaaa aacagatgtt tatatctata acctaggctc gggagaaggc
     1921 actagtgtat tacaacttgt aaatacattt gaaagtgtta ataagatccc tattccttat
     1981 aaaatagttc caagacgttc aggagacgtt gcaacttgtt atgcaaatgc agacaaagca
     2041 tataaggaat taaattggag gacaacaaaa tcgattgaag acatgtgtag agatacatgg
     2101 aattggcaat caaaaaatcc caatggctat aattgatgat actgtctata tgagtgttgt
     2161 attttgggaa aattacttaa ccttttaatg aaagataatt ttttaaaaaa atgtttgaaa
     2221 gcgctt
//
LOCUS       DAVHIU010000171         2141 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVHIU010000171 DAVHIU010000000
VERSION     DAVHIU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.173"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1876
                     /locus_tag="VVN06_001986"
     CDS             <1..1876
                     /locus_tag="VVN06_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU5563462.1"
                     /translation="SKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQY
                     LRDSCFIENFGVEHTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLL
                     DEKNIELLLSKAPYLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLF
                     DKRVYFNEWVEYHDFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRH
                     FGVSLQSGFNSFTIIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQF
                     ENDVIFIIAGTNASVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFV
                     KPDVSYYGGGNGDFINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALL
                     IDAAIPWNDKKTFTDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFP
                     VPISSESYPYVAKATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAE
                     DTPGYVRENAARNIFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSG
                     DGQGVRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE
                     "
     gene            1927..>2141
                     /locus_tag="VVN06_001987"
     CDS             1927..>2141
                     /locus_tag="VVN06_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5563463.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLT"
BASE COUNT          722 a          274 c          427 g          718 t
ORIGIN      
        1 aagtaagata attgaagttt ttgaggaaca ttttgataga gcgataacat gtgagatgtt
       61 ttctgattca tcaacgtttg cttctataaa tttttcggag tatggtattt caaaaagcaa
      121 atttcaacag tatttgagag attcttgctt tattgagaat tttggagttg agcacactac
      181 tgtttctgat attcaaaatt ctatcgtgac attttatgat gtccatacag acatttttag
      241 attactgaat aaattaaata ttgatatttc agaagctaat atcatgaatc agaccacggt
      301 cttgcttgat gaaaaaaata ttgaattgct actatcaaaa gcgccctatc ttgtctccat
      361 gattgtggaa gatttttcta agttatctgt agatgatttt tctttagata ataatgattt
      421 aaaaataaat cttccatctc caatgaacga accagttgtt ggcgttattg ataccttatt
      481 tgacaaaaga gtttacttta atgagtgggt agaatatcat gattttgtct cacctgatat
      541 tagcaaagat agccaagatt acaagcatgg gacagctgtg acttctttaa tcgttgatgg
      601 agccaatttg aaccctaatc tggatgatgg ttgtggaaat tttagagtga ggcattttgg
      661 tgtgtcacta caaagtggat ttaattcatt tacaatcata aaacagataa aagagattgt
      721 ttctcagaat gctgatatta aggtttggaa tctttcttta gggtccaatg atgaaataag
      781 agaaaatttt atctctgcag agggagcatt attagacgaa attcaatttg agaatgatgt
      841 aatttttatc atagctggga caaatgcttc agtaataaat gggaaacgca aaagaatagg
      901 tgctcctgct gattcactta attctataat agtaaattct gttgatttta ataatcaatc
      961 agtgtcatat tcacgagagg gtattgtttt atcattcttt gttaaaccag atgtgtcata
     1021 ttatggagga ggaaatggtg actttataaa tgtttgtgaa cctctaggat taggtcgtgt
     1081 agctggaaca agttttgcgg caccttttat tgcaaggaaa atggcctatc tcattcatat
     1141 tatgggactt agccgagaag aagcgaaggc tttgctaatt gatgcagcaa taccctggaa
     1201 tgataagaaa acattcactg atttatcttt gataggaaat ggtattgttc caataaaaat
     1261 ggatgatatt ttatctacac ctgatgatga aattaaattt attgtgtcag atatctctag
     1321 ggcttatgat acctataatt atgattttcc agtacctatt tctagtgaga gttatccata
     1381 tgtagctaaa gcaacaatgt gttatttccc taactgctca agaaaacaag gggttgatta
     1441 tacaaatact gagatgcagc ttacttttgg taggttaaag tcagacggta tcaaatcgat
     1501 aaataaagac aaccagcatg ccgaagatac cccaggatat gtgagagaaa acgctgctag
     1561 aaatatattt aggaaatggg ataatgtcaa gcatattgga gagtcgttta cttctcgaaa
     1621 gagagctaaa gcaattttaa atccatctaa tcctcagtgg gggatgagta ttaaaacaat
     1681 cgaacgtttg aaaagcggtg atggacaagg agtacgtttt ggtgtagtag ttacattaaa
     1741 agaattaaat ggggttaatc gtattgaaga ttttattcag caagctgaat tacgcggttg
     1801 gctagttaat agattgcaag tagaagctca agtagacctc tttaatagtt taaatgaaga
     1861 aattgaattt gaatagtgga tattttatcc ttattttaga tcttaggaac acaagagggc
     1921 aaagatatga atattactta tatagttgga aatggtttgg atttgcaata tggtttgaaa
     1981 acaagatata gggatttcta cgaattccaa aataaggttt atataagtag gacagagaac
     2041 gaggaaaaat attcaaattt catttatgag tctctatttt ctgataaagt aaatgattat
     2101 gaaaattggt cagattttga attgtcaatt ggtaaattga c
//
LOCUS       DAVHIU010000172         2073 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVHIU010000172 DAVHIU010000000
VERSION     DAVHIU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.174"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            453..1589
                     /gene="dnaJ"
                     /locus_tag="VVN06_001988"
     CDS             453..1589
                     /gene="dnaJ"
                     /locus_tag="VVN06_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5563464.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEVKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1911..>2073)
                     /locus_tag="VVN06_001989"
     CDS             complement(1911..>2073)
                     /locus_tag="VVN06_001989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563465.1"
                     /translation="KIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHDKPTLTWFEEIF
                     EEYHSPFHN"
BASE COUNT          589 a          399 c          481 g          604 t
ORIGIN      
        1 tgactgtgga aagaaatgga aatgtcattt ataaagaatt tacagaagta gctgttggta
       61 agagtaaata tgactacact tctgtgttta aagatgttat gatttacttt tcggatcata
      121 tatgtcagga gttacaccta tataaaacac aagacccaca ctataataaa tttgagtttg
      181 atttagctaa aaggtctttg aagattacta ctagagataa tacaaccatt tttatttcaa
      241 atgaataatc taaccacaga ggttgcaacc cagcctctgt ttttcggtaa aaagggatag
      301 ggccgagtgg ctggagtatt atctcatcaa tataaaagaa aggaattgaa cccgacctaa
      361 attctcggaa aaaagagaaa tctgtctagg agcgtcgctc cagcgtcaga tttcctattt
      421 ttcagtcgaa ttttacggtc ttggtatctt gtatgaacaa tactgaattt tatgatcgtc
      481 tgggggtgtc aaaaaacgct tcggcagacg agatcaaaaa ggcttatcgt aagctttcca
      541 aaaaatatca cccagatatc aacaaggagc ctggtgctga ggacaagtac aaggaagttc
      601 aagaagccta tgagactttg agtgacgacc aaaaacgtgc tgcctatgac cagtatggtg
      661 ctgcaggcgc caatggtggt tttggtggag ctggtggttt cggtggtttc aatggggcag
      721 gtggcttcgg tggttttgag gatattttct caagtttctt cggcggaggc ggttcttcgc
      781 gcaatccaaa cgctcctcgc caaggagatg atctccagta tcgtgtcaat ttgacatttg
      841 aagaagctat cttcggaact gagaaggaag ttaagtatca tcgtgaagct ggctgtcgta
      901 catgtaatgg ctctggtgct aagccaggga caagtccagt cacttgtgga cgctgtcatg
      961 gcgctggtgt cattaacgtc gatacgcaga ctcctcttgg tatgatgcgt cgccaagtaa
     1021 cctgtgatgt ctgtcacggt cgaggaaaag aagtcaaata tccatgtaca acctgtcatg
     1081 gaacaggtca tgagaaacaa gctcatagcg tacatgtgaa aatccctgct ggtgtggaaa
     1141 caggtcaaca aattcgcctc gctggtcaag gtgaagcagg ctttaacggt ggaccttatg
     1201 gtgacttgta tgtagtagtt tctgtagaag ctagcgacaa gtttgaacgt gaaggaacga
     1261 ctatcttcta caatctcaac ctcaactttg tccaagcggc tcttggtgat acagtagata
     1321 ttccaactgt tcacggtgat gttgaattgg ttattccaga gggaactcag actggtaaga
     1381 aattccgcct acgtagtaag ggggcaccga gccttcgtgg cggtgcagtt ggtgaccaat
     1441 acgttactgt taatgtcgta acaccgacag gcttgaacga ccgccaaaaa gtagccttga
     1501 aagaattcgc gactgctggt gacttgaaag taaatccaaa gaaaaaaggc ttctttgacc
     1561 atataaaaga tgcctttgat ggagaataat actcttcgaa aatctcttca aaccacgtca
     1621 gcgttgcctt gccgtatata tgtgagtcgt atctacaacc tcaaaacagt gttttgagca
     1681 gcccgtggct agtttcctag tttgcttttt gattttcatt gagtataaac aaaaagagct
     1741 gtcgggctct ttttacttta tagatttttt aagactttcc taagtaatga cggacggtag
     1801 cgacctcctt cgaagttcca tacctaaact ttgaacctaa gtcttaaagt ttccggacag
     1861 ctgaaaccaa gctgtttcag gtgttttcat tacggcagaa agtcttcgat ttagttgtga
     1921 aatggtgaat gatactcttc aaaaatttct tcaaaccacg tcagcgtcgg cttgtcatgg
     1981 gtatggttac tgacttcgtc agttctatcc acaaccttaa aacggtgttt tgagcagtct
     2041 gtgcctagct ttctagtttg ctttttgatt ttt
//
LOCUS       DAVHIU010000173         2042 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVHIU010000173 DAVHIU010000000
VERSION     DAVHIU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.175"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..165
                     /locus_tag="VVN06_001990"
     CDS             <1..165
                     /locus_tag="VVN06_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563466.1"
                     /translation="IISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIE
                     KTKMAEEFIY"
     gene            176..760
                     /locus_tag="VVN06_001991"
     CDS             176..760
                     /locus_tag="VVN06_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU5563467.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLIIYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            760..1455
                     /locus_tag="VVN06_001992"
     CDS             760..1455
                     /locus_tag="VVN06_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU5563468.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            1443..>2042
                     /locus_tag="VVN06_001993"
     CDS             1443..>2042
                     /locus_tag="VVN06_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563469.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIY"
BASE COUNT          709 a          267 c          386 g          680 t
ORIGIN      
        1 atcatttcac atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac
       61 ggaagagtag aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa
      121 aatttaatag aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga
      181 taataaaaaa ttaaaagtaa aagatttagt aagtatcggt gtttttggcg taatttattt
      241 tgccttcatg tttggagttg gtatgatggg cttgattcca atattgttct taatataccc
      301 gacagtatta gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa
      361 gccatgggca ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca
      421 tacctacgta gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa
      481 gattggtaat tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg
      541 gatatgtagc tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt
      601 gatgacgatg ggaaaagatt atgttgatgt attagaaaag ttaataattt atcctcacat
      661 ggctttagta gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc
      721 tctattgaaa aaacactttg aaaaagcagg catcgtataa tgcctacttt ttcttcaaat
      781 gctcccttta atcccaatcc gatctctaag atattagtcg tatttatcac aggcttaact
      841 gttatgcata gcataaacat ccgttttgag ctggcaattg tttgtcttat tggtattttg
      901 ctttatttga atggatacaa aaaaacactt ttcaaatgga ttatattgtg tgggatactt
      961 tactccttgc ctaattttat ggtgttatct gaattaaacc ctatagttaa gatgtttttg
     1021 agtataccta ttgttattag aatgtttatt ttaccattta tggcagcaag ctttatgata
     1081 aagacctcgg atgtaggcgc aataatttca tcgatggata agcttaagat ttcaaagaat
     1141 gtatccatac ctattgcggt tatgtttaga ttcttcccat cttttaagga ggagaagaaa
     1201 aacatcaaaa tggctatgag agtaagaggg ataaatttta aaaacccagt caaatatctt
     1261 gaatatgttt ctgtgccact actcattata tcatctaata tatcagatga cattgcaaaa
     1321 gcggcagaaa caaaggcaat agaaaatcca attgccaaga ccagatacat tcgcgtaaag
     1381 atacagctaa ttgattttgt ttatgtttta gcggttgctg gacttattgt gggaggctta
     1441 atatggttga aataaaaaat ttaagtcttg attatggtga agagcatata ttagatgata
     1501 tatcactatc catagccgag ggagagtgcg tgctatttac aggaaaaagt ggaagtggta
     1561 agtcatcttt aataaattca atcaatggac tagctgtaag gtatgataac gcaaagacaa
     1621 agggcgaaat aattattgat ggtaagaata taaaaaattt ggaactttat caaatctcaa
     1681 tgcttgtttc aactgttttt caaaatccta agacatattt ttttaatgtc aatacgacat
     1741 tagaattatt attttatttg gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt
     1801 tgaaggatat gcttgagata ttcccgataa aaaatctttt gaacagaaat atatttaatc
     1861 tatccggcgg tgaaaaacaa attctttgca ttgcagcttc ttatatagca ggtacaaaga
     1921 ttatagttat ggatgagtct tcatcgaatt tagatattaa aagcataagt gttttggcaa
     1981 agatgctaaa gatattaaaa gagaaaggca taagcataat tgttgcagag catagaattt
     2041 at
//
LOCUS       DAVHIU010000174         1967 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVHIU010000174 DAVHIU010000000
VERSION     DAVHIU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.176"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(249..1739)
                     /gene="lysS"
                     /locus_tag="VVN06_001994"
     CDS             complement(249..1739)
                     /gene="lysS"
                     /locus_tag="VVN06_001994"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5563470.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINELFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLFFPTMK"
     gene            complement(1867..>1967)
                     /locus_tag="VVN06_001995"
     CDS             complement(1867..>1967)
                     /locus_tag="VVN06_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU5563471.1"
                     /translation="TYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT          555 a          397 c          410 g          605 t
ORIGIN      
        1 gtgaatctga acaaatcaat tggggaatcc aagtcaattt ctagtacttt ttgaacagtt
       61 tcagcgtact atcgtaaaat gaaataaaac atgcacaaat tgattaggga atttaaacca
      121 atttctaaca atgtcttaga aatcaaagtg tactatttca acttcaatat actacacttc
      181 attttctcct taaattcagt ctctttttga atcaaaaaat cctctggtaa gagccagagg
      241 ataagaattt atttcattgt tgggaagaac aatacatcac ggatagttgt tgtatcagtg
      301 aggagcatgc agagacggtc gataccgatt cccaaaccac ctgttggtgg cataccgtat
      361 tcaagagctt caatgtagtc atagtcgatt cctgtcgctt catcatcacc aagttctttg
      421 gctttagctt gggcttcaaa acggctaagt tggtcgattg ggtcgttgag ttcagtaaag
      481 gcattaccgt actccttagt catgataaag agctcgaaac ggtcagtaaa gcgttggtct
      541 tcaggatttt tcttagcgag tggagataca gctactggat gtccatagac aaaggttggt
      601 tggattaaag tttcttcaac aaactcttca aagaaggcat tgatgatgtg accaacctca
      661 gtgtagtgtt tctcaactgg aactttcttc tcagcagcga tagctttagc ttcttctaaa
      721 gtcatgtctt gccagaaatc gacaccagta atttctctga tagcatccac catatgaaca
      781 cgcttaaata gttcgttaat cttgatttca gtaccttggt agttgactgg gccatcgccc
      841 ttaactgatt tagcagcgtg ttggataatg ccttccgtca agtccatgat gtcttggaag
      901 tctgcataag cttggtaaac ttcgatagaa gtaaactcag ggttatgagt agcgtccatt
      961 ccttcgttac ggaagatacg gccaatttca tagacacgtt ccataccacc cacgataagg
     1021 cgttttaagt gaagctcagt cgcgatacga agcaccatgt caatgttttg ggcattgtgg
     1081 tgggtgataa atggacgggc agcagcacca ccggcttcat tatgaagaac aggtgtttcc
     1141 acttcaagga atcctttttg gtcaaggtaa cgacggattt cagagatgat ttttgaacga
     1201 gtgacaaagc gttcaaagct ttcacggtta gaaatcaagt caaggtaacg tttacggtaa
     1261 attgtttcaa cgtctgtcaa accatggaat ttctcaggaa gaggacgaag agccttagac
     1321 aagtgtgtga tgtgggttgc cttgatagag agttctccca tatccgtacg catcacttca
     1381 ccttcgacac caaggaagtc accaaggtct gcttttttga agatttcgta gttttcttca
     1441 ccgacagcat ccttacgaac gtagatctga atctggcctt cgcggtcttg aaggtgggca
     1501 aaaccaactt ttcctttacc acgtttggtt atcaagcgtc ctgcgatagt agctgtttcg
     1561 tttttatcgt gtaattgttc tttatcgagg ttggcatatt tatcttttaa ttcttgtgaa
     1621 tttgcagtac gttcaaaacg ttttccgaaa ggatcgattc cttgttcgcg gagcgcagcc
     1681 attttttcac ggcgaacgat ctgctggtca tttagttctt ccatatgttc tgtagacatg
     1741 ggatcctcct agttttactc aaaattgaat acgatgagtc attttttgcg atactcccat
     1801 tttatcataa ataagcaaga aattcacgga taatctttaa aaactaaaaa gaacctgacg
     1861 ctaaaatcac tggaaagaag tgaaagtatc tcccgcccca ctagcgatat agccaaatgc
     1921 tgctttagga ataacttgtt gcgccattgg ctccaaatca taggtat
//
LOCUS       DAVHIU010000175         1923 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVHIU010000175 DAVHIU010000000
VERSION     DAVHIU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.177"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            96..1766
                     /locus_tag="VVN06_001996"
     CDS             96..1766
                     /locus_tag="VVN06_001996"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5563472.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT          541 a          372 c          445 g          565 t
ORIGIN      
        1 agagatattt acgaacgttt agagagtttt aggatgttaa aaatcttgtt ttgtgatata
       61 atgaattatc atataagagg ttagaggaat tttgaatgaa aacagatatt gaaatcgcac
      121 agagtattga gttgaagcca attgttgatg ttgtagagaa acttggtatt tcttacgacg
      181 atttggagtt gtacggaaag tacaaggcta aactcagctt tgataaaatt cgtgcagttg
      241 agagcaatcc agtcggtaaa ttgatcttgg ttactgccat caacccaaca cctgcaggtg
      301 aaggaaagtc gacgcttacc attggtcttg cggatgcctt gaacaagatt ggcaagaaaa
      361 ccatgattgc tatccgcgaa ccgtctcttg gtccagtcat ggggatcaag ggtggtgctg
      421 ctggtggtgg gtatgcacaa gttctgccaa tggaagacat caacctccac tttactggag
      481 atatgcatgc tattacaact gccaacaatg ccctttctgc cttgattgac aaccacttgc
      541 accaagggaa tgagctggga attgatcaac gtcgtatcct ctggaaacgc gttgtggact
      601 tgaacgaccg tgcgctccgc catgtgactg ttggtcttgg tggtcctcta aacggtattc
      661 cacgtgagga tggttttgat attacagttg cttcagaaat catggcaatt ctttgcttgg
      721 caacggacat cgaggacttg aaacgtcgtt tggcgaatat cgttattggt tatcgctatg
      781 accgtacgcc tgtttctgta ggtgatttgc aggttgaggg tgccttggct ttgattttga
      841 aggatgctat taagccaaac ttggttcaga caatttacgg tacacctgcc tttgtacacg
      901 gtggtccatt tgccaatatc gctcatggct gtaactctgt tttggcgacg acaacagccc
      961 ttcacttggc tgattacact gttactgaag ctggttttgg tgcggacctt ggtgctgaga
     1021 aattccttga tatcaagaca ccaaacttgc caacatctcc agatgcagtt gttattgtcg
     1081 caacccttcg tgcccttaag atgaatggtg gtgtggctaa agacgctctg actgaagaaa
     1141 atgtagaggc agttcgtgca ggttttgcta acttgaaacg ccacgttgaa aatatccgta
     1201 agttcggtat tccagcagtt gtagctatta acgaatttgt atctgataca gaagctgaaa
     1261 ttgcagcctt aaaagaactc tgtgcctcaa tcgatgtacc agttgaattg gctagtgtct
     1321 gggctgatgg agcagaaggt ggagtagcac ttgccgaaac agttgttaag acaattgctg
     1381 aaaatccagc taactataaa cgtttgtacg ataatgacct ttctgtccaa gaaaagattg
     1441 aaaaaattgt cactgaaatc tatcgtggta gcaaagtgaa ctttgagaag aaagctcaaa
     1501 cacaaattgc tcaaatcgtt caaaacggtt gggacaaatt gccaatttgt atggctaaaa
     1561 ctcaatacag tttctcagac aatccaaatg cgcttggagc accagaaaac tttgaaatta
     1621 ccattcgtga attggtacca aaattaggtg caggcttcat cgttgcctta actggtgatg
     1681 ttatgaccat gccaggtctt ccaaaacgtc cagcagctct caacatggat gttgaaagcg
     1741 atggaactgt actaggcttg ttctagtgga ttgaaattga ctttaagtga gtttggaaat
     1801 aatatccaag ctcatttttt ctcagactgc aagcagttca tcaataagat ttatactctt
     1861 cgaaaatctt ttcaaactac gtcagcttca ccttgccgta ggtaatatga tactgactct
     1921 gtc
//
LOCUS       DAVHIU010000176         1896 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVHIU010000176 DAVHIU010000000
VERSION     DAVHIU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.178"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            125..1756
                     /locus_tag="VVN06_001997"
     CDS             125..1756
                     /locus_tag="VVN06_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU5563473.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIGLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          552 a          397 c          366 g          581 t
ORIGIN      
        1 aaataataca cttatcaatt ataattttct tttacaaact tgacattaac acttgttaga
       61 tttactttta tcctttaaaa tagaatagga gatattccgc gattattttt cggaggaaaa
      121 agaaatgtcc attaattggc aggaaatttt atttcacttt ttaggtggtc tggggctatt
      181 cttatatagc atcaagacca tgggagacgg tttacaacaa gctgctggag atcgccttcg
      241 tttttacatt gacaaatata ctagtaatcc tttctttgga gttctggttg gtattgggat
      301 gactgctcta attcagtcta gttctggtgt aacagttatt acagtcggcc tggtcagtgc
      361 cggtctctta accttacgtc aggctatcgg gattgtcatg ggtgctaata ttgggacaac
      421 tgtcacatcc tttctcatcg gttttaaatt aggtaactat gccctaccta tgctctttat
      481 cggtgccgtc tgtctctttt ttacaaaaaa tcggacagtc aataatatcg gacgcatcct
      541 ctttggtatc ggtggtatct tttttgccct caatctcatg agcggcgcaa tggctccact
      601 caaggattta caggtcttta aggactatat gattgagcta agtaagaatc ctgttttggg
      661 tgtctttgtc ggtactggct tgaccttgct aattcaagct tcttcggcta ccattgggat
      721 tttacaaaac ctctacgccg gcaatctaat tgacctacag ggagctttgc cagttctatt
      781 tggtgacaat atcgggacaa ccattacagc catcattgcc tctttagggg ctaatattgc
      841 agctaaacgg gtagcaggag ctcatgttgc cttcaacgtt atcggaacag tcgtctgcgt
      901 tatttttcta gttcctttta ctgtcctgat tcattggttt gaagctacgc taaatctagc
      961 accggaaatg accatcgcct ttgctcacgg aacctttaat attaccaaca ccattgtcca
     1021 atttccattt atcggagctc tggcttactt tgtaaccaag attattcctg gagaggacga
     1081 ggttgtcaaa tacgaaccct tatatcttga tgaacatttc atcaaacagg ccccatctat
     1141 cgctctagga aatgctaaga aagagctctt gcacttagga aactacgctg ctaaagcctt
     1201 tgacctttcc tataagtaca tcattggctt ggatgaaaaa gttgctgaaa aagggcataa
     1261 aaccgaagaa gcaattaaca ccatcgatga gcaattaaca cgttatctca ttgccctttc
     1321 aagcgaagct ctcagccaaa aagaaagtga agtgcttacc aatatccttg attcctcccg
     1381 tgatttggaa cggattggag accacacgga ggctctactc aatctgactg actatcttca
     1441 acggaaaaat gttgaatttt ctgatgccgc cttgaaagaa ttagaggaag tttaccgcca
     1501 aactagtgac tttatcaaag atgctctgga tagtgtggaa aacaatgata ttgaaaaagc
     1561 acgcagtctt gtagaacgtc atgaagcaat caataagata gaacgtgttc tcagaaaaac
     1621 ccacatcaaa cgcctcaaca aaggcgaatg ttcaacacaa gctggggtca actttatcga
     1681 catcatctca cactacactc gtgtatcaga ccacgctatg aaccttgctg aaaaggtttt
     1741 tgcagaacaa atctaagaac caagaagcta tccatcataa ttggatggct tcttactttt
     1801 tcctaagcaa gactaggctg aatgaaactg aaagagtatt ctgcagatat atagtatatt
     1861 gaaactagaa tagtacacct ctatttctaa aacatt
//
LOCUS       DAVHIU010000177         1851 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVHIU010000177 DAVHIU010000000
VERSION     DAVHIU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.179"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1125
                     /locus_tag="VVN06_001998"
     CDS             <1..1125
                     /locus_tag="VVN06_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5563474.1"
                     /translation="ISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQ
                     AVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNN
                     HVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSEKVTTVAEFGDSSKLTVGE
                     TAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGP
                     LINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQM
                     VNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHLEKYDVITKVDDKEIASSTD
                     LQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES"
     gene            1183..>1851
                     /locus_tag="VVN06_001999"
     CDS             1183..>1851
                     /locus_tag="VVN06_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5563475.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEACAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVE"
BASE COUNT          672 a          333 c          346 g          500 t
ORIGIN      
        1 attagctttt ttagtggagc cttgggtagt ttttcaataa ctcaactaac tcaaaaaagt
       61 agtgtaaaca actctaacaa caatagtact attacacaaa ctgcctataa gaacgaaaat
      121 tcaacaacac aggctgttaa caaagtaaaa gatgctgttg tttctgttat tacttattcg
      181 gcaaacagac aaaatagcgt atttggcaat gatgatactg acacagattc tcagcgaatc
      241 tctagtgaag gatctggagt tatttataaa aagaatgata aagaagctta catcgtcacc
      301 aacaatcacg ttataaatgg cgccagcaaa gtagatattc gattgtcaga tgggactaaa
      361 gtacctggag aaattgtcgg agctgacact ttctctgata ttgctgtcgt caaaatctct
      421 tcagaaaaag tgacaacagt agctgagttt ggtgattcta gtaagttaac tgtaggagaa
      481 actgctattg ccatcggtag cccgttaggt tctgaatatg caaatactgt cactcaaggt
      541 atcgtatcca gtctcaatag aaatgtatcc ttaaaatcgg aagatggaca agctatttct
      601 acaaaagcca tccaaactga tactgctatt aacccaggta actctggcgg cccactgatc
      661 aatattcaag ggcaggttat cggaattacc tcaagtaaaa ttgctacaaa tggaggaaca
      721 tctgtagaag gtcttggttt cgcaattcct gcaaatgatg ctatcaatat tattgaacag
      781 ttagaaaaaa acggaaaagt gacgcgtcca gctttgggaa tccagatggt taatttatct
      841 aatgtgagta caagcgacat cagaagactc aatattccaa gtaatgttac atctggtgta
      901 attgttcgtt cggtacaaag taatatgcct gccaatggtc accttgaaaa atacgatgta
      961 attacaaaag tagatgacaa agagattgct tcatcaacag acttacaaag tgctctttac
     1021 aaccattcta tcggagacac cattaagata acctactatc gtaacgggaa agaagaaact
     1081 acctctatca aacttaacaa gagttcaggt gatttagaat cttaattgac atctatgtaa
     1141 agaaagcttt acataagaga aaagatgtgt tagtgtagaa tcatggaaaa atttgaaatg
     1201 atttctatca cagatataca aaaaaatccc tatcaacccc gaaaagaatt tgatagagaa
     1261 aaactagatg aactagcaca gtctatcaaa gaaaatgggg tcattcaacc gattattgtt
     1321 cgtcaatctc ctgttattgg ttatgaaatc cttgcaggag agagacgcta tcgggcttca
     1381 cttttagctg gtctacggtc tatcccagct gttgttaaac agatttcaga ccaagagatg
     1441 atggtccagt ccattattga aaatttacag agagaaaatt taaacccaat agaagaagca
     1501 tgcgcctatg aatctctcgt agagaaagga ttcacccatg ctgaaattgc agataaaatg
     1561 ggcaagtctc gtccatatat cagcaactcc attcgtttac tttccttgcc agaacagatt
     1621 ctctcagaag tagaaaatgg caaactatca caagcccatg cgcgttccct agttgggtta
     1681 aataaggaac aacaagacta tttctttcaa cggattatag aagaagatat ttctgtaagg
     1741 aagttagaaa ctcttctgac agagaaaaaa caaaagaaac agcaaaaaac taatcatttc
     1801 atacaaaatg aagaaaaaca gttaagaaaa ctactcggat tagatgtaga a
//
LOCUS       DAVHIU010000178         1808 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVHIU010000178 DAVHIU010000000
VERSION     DAVHIU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.180"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            154..1647
                     /locus_tag="VVN06_002000"
     CDS             154..1647
                     /locus_tag="VVN06_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU5563476.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHRLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
BASE COUNT          612 a          278 c          370 g          548 t
ORIGIN      
        1 cctacggcaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat aaaatattca
       61 ggtgacgcat agatatagtt aattgaagct ttgtttgaaa tctgataaaa taatgatatt
      121 actaagtttt aaaaactaaa gaaaagggaa gatatgatta caggcgaatt aaaaaataaa
      181 atcgatcagc tgtgggaaat tctttggaca gaaggaaacg caaatccttt aacaaatatt
      241 gaacagttga cttatctctt atttatgaaa gatttggata gtgtcgagct tggacgtgaa
      301 agtgatgctg aatttctagg gattccttat gagggagttt ttccaaaaga taaacctgaa
      361 taccgttggt caacttttaa aaatatagga gatgctcagg aagtttatcg tttaatgact
      421 caggagattt ttccgtttat taaaaatctc aagggggata cagatgatac agccttttca
      481 cgatatatgc gagaagctat ttttcaaata aataaacctg ctacgcttca aaaggcaatt
      541 tctatcttag atgtttttcc aactagggga ttagatgtag attttgataa tgacaaacaa
      601 agtattactg atatcggaga tatctatgaa tatctgttat caaaattgtc gaccgcaggt
      661 aaaaatggac agttccgtac acctcgtcac atcatcgata tgatggttga gttgatgcaa
      721 ccgactatca aagatatcat ctcagatccc gctatgggtt ctgctggctt cttagtatct
      781 gctagccgtt acttaaagcg taagaaagat gaatgggaaa ccaatacaga taatatcaat
      841 cattttcata atcagatgtt tcatggaaat gatacggata cgactatgtt gagacttggg
      901 gcgatgaaca tgatgctaca tggagtagaa aatccacaaa tcagttacct tgactcgctg
      961 tctcaagata atgaagaagc cgataaatat actttggttt tagcaaatcc tccttttaag
     1021 ggctcacttg actacaattc aacctctaat gaccttcttg caaccgtaaa aaccaaaaaa
     1081 acagaattac tctttctttc tcttttcttg cgaactttaa aaccaggtgg acgagcagca
     1141 gttatcgtac ctgatggtgt cctttttggt tcgtctaaag ctcataaagg aattcgtcag
     1201 gaaattgtag agaatcatag gcttgatgct gtaatctcaa tgcctagtgg tgtgttcaag
     1261 ccttatgctg gagtttcaac tgccattctc atctttacaa aaactggtaa tggtggtact
     1321 gacaaagtct ggttttacga tatgaaagcg gatggtttaa gtttggatga taagcgacaa
     1381 ccgattagcg acaatgatat tccagatatt atcgaacgct ttcatcatct tgaaaaagaa
     1441 gcagaacgtc agagaacgga tcaatctttc tttgttccag ttgctgagat aaaggaaaat
     1501 gattatgatt tgtctatcaa taaatataaa gagattgagt atgaaaaagt tgagtatgaa
     1561 ccaacagaag tcatattaaa gaaaatcaat gatttagaaa aagaaattca agctggcttg
     1621 gctgaattgg aaaaattact caagtaggga ggtggctgta tgaaaaaagt gaagttgggg
     1681 gaagtttgtg agattttatc tggttacgct tttaaatcat cacaatttaa tgataacaaa
     1741 attggtctcc ccttaatacg aattcgagat gtagaaagag gtttttctga tacctacttt
     1801 gaaggaac
//
LOCUS       DAVHIU010000179         1748 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVHIU010000179 DAVHIU010000000
VERSION     DAVHIU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.181"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>1748
                     /gene="phtE"
                     /locus_tag="VVN06_002001"
     CDS             <1..>1748
                     /gene="phtE"
                     /locus_tag="VVN06_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU5563477.1"
                     /translation="AVIVSLSLCAYALNQHRSQENKDNNRVSYVDGSQSSQKSENLTP
                     DQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSEELLMKDPNYQLK
                     DADIVNEVKGGYIIKVDGKYYIYLKDAAHADNVRTKDEINRQKQEHVKDNEKVNSNVA
                     VARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLSASELAAAKAHLA
                     GKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELYDSPSAQRYSESD
                     GLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPISGTGSTVSTNAK
                     PNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAYIVRHGDHFHYIP
                     KSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRIIAEDESGFVMSHG
                     DHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSNAKEMKDLDKKIE
                     EKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVGIGHSHSNYELFK
                     PEEGVAKKEGNKVYTG"
BASE COUNT          608 a          328 c          345 g          467 t
ORIGIN      
        1 agctgttatc gtatccttga gtctatgtgc ctatgcacta aaccagcatc gttcgcagga
       61 aaataaggac aataatcgtg tctcttatgt ggatggcagc cagtcaagtc agaaaagtga
      121 aaacttgaca ccagaccagg ttagccagaa agaaggaatt caggctgagc aaattgtaat
      181 caaaattaca gatcagggct atgtaacgtc acacggtgac cactatcatt actataatgg
      241 gaaagttcct tatgatgccc tctttagtga agaactcttg atgaaggatc caaactatca
      301 acttaaagac gctgatattg tcaatgaagt caagggtggt tatatcatca aggtcgatgg
      361 aaaatattat atctacctga aagatgcagc tcatgctgat aatgttcgaa ctaaagatga
      421 aatcaatcgt caaaaacaag aacatgtcaa agataatgag aaggttaact ctaatgttgc
      481 tgtagcaagg tctcagggac gatatacgac aaatgatggt tatgtcttta atccagctga
      541 tattatcgaa gatacgggta atgcttatat cgttcctcat ggaggtcact atcactacat
      601 tcccaaaagc gatttatctg ctagtgaatt agcagcagct aaagcacatc tggctggaaa
      661 aaatatgcaa ccgagtcagt taagctattc ttcaacagct agtgacaata acacgcaatc
      721 tgtagcaaaa ggatcaacta gcaagccagc aaataaatct gaaaatctcc agagtctttt
      781 gaaggaactc tatgattcac ctagcgccca acgttacagt gaatcagatg gcctggtctt
      841 tgaccctgct aagattatca gtcgtacacc aaatggagtt gcgattccgc atggcgacca
      901 ttaccacttt attccttaca gcaagctttc tgccttagaa gaaaagattg ccagaatggt
      961 gcctatcagt ggaactggtt ctacagtttc tacaaatgca aaacctaatg aagtagtgtc
     1021 tagtctaggc agtctttcaa gcaatccttc ttctttaacg acaagtaagg agctctcttc
     1081 agcatctgat ggttatattt ttaatccaaa agatatcgtt gaagaaacgg ctacagctta
     1141 tattgtaaga catggtgatc atttccatta cattccaaaa tcaaatcaaa ttgggcaacc
     1201 gactcttcca aacaatagtc tagcaacacc ttctccatct cttccaatca atccaggaac
     1261 ttcacatgag aaacatgaag aagatggata cggatttgat gctaatcgta ttatcgctga
     1321 agatgaatca ggttttgtca tgagtcacgg agaccacaat cattatttct tcaagaagga
     1381 cttgacagaa gagcaaatta aggctgcgca aaaacattta gaggaagtta aaactagtca
     1441 taatggatta gattctttgt catctcatga acaggattat ccaagtaatg ccaaagaaat
     1501 gaaagattta gataaaaaaa tcgaagaaaa aattgctggc attatgaaac aatatggtgt
     1561 caaacgtgaa agtattgtcg tgaataaaga aaaaaatgcg attatttatc cgcatggaga
     1621 tcaccatcat gcagatccga ttgatgaaca taaaccggtt ggaattggtc attctcacag
     1681 taactatgaa ctgtttaaac ccgaagaagg agttgctaaa aaagaaggga ataaagttta
     1741 tactggag
//
LOCUS       DAVHIU010000180         1734 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVHIU010000180 DAVHIU010000000
VERSION     DAVHIU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.182"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            103..1131
                     /gene="queA"
                     /locus_tag="VVN06_002002"
     CDS             103..1131
                     /gene="queA"
                     /locus_tag="VVN06_002002"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5563478.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
BASE COUNT          497 a          396 c          327 g          514 t
ORIGIN      
        1 tttttgtcaa gtgaaaacga tttaatttct aaaaaaagag cgtccaaact tgaaacgtgt
       61 tataatggat aggattagaa cttagaaaga atgaacagat atatgaatac agctgatttt
      121 gatttccact tgccagagga attgattgcc caaacgcccc ttgaaaaacg tgatgcctcc
      181 aaactcctca tcgtcaaccg tgagacagga gaaatgcaag ataaacactt ccactctatt
      241 attgatatgc tggaacctgg tgatgccctt gtcatgaacg acacccgagt tctccctgcc
      301 cgcctctatg gtcaaaaagt ggagacagga ggtcatgtgg aacttctcct ccttaagaac
      361 actagtggag acgagtggga agttctggct aaacctgcca aacgcctcaa ggtcggtact
      421 cgtatcagct ttggtgatgg ccgcctcagc gctgtcgtta cagaagaatt gacccacggg
      481 ggacgcattg tccgctttga ataccaagga attttcctag aagtcttgga aagtctggga
      541 gaaatgcctc tgccacctta tatccacgaa aaattagatg accgtgaacg ttatcaaacc
      601 gtctacgcca aggaaagcgg ctctgctgca gcaccgactg ctggtcttca cttcaccaaa
      661 gaactgctgg cagaaatcca agctaagggt gttcatctag tctatctgac tctccatgtc
      721 ggactcggaa cctttagacc tgtttctgtg gataatctgg acgaacacga aatgcactca
      781 gagttctatc aactttctga ggaagctgct gccacccttc gctctgtcaa aaaaaatggt
      841 ggtcgtgtca tcgctgtcgg aaccacttct atccgcacct tggaaactat tggttccaag
      901 tttgatgggc aaatccaagc agattctggt tggaccaata tctttatcaa acctgggtat
      961 gagtggaagg tcgtggatgc cttctcaacc aacttccacc tgccaaaatc aactctggtc
     1021 atgttggttt ctgcctttgc aggccgtgaa ttagtcttag atgcctacca ccattccatc
     1081 caagaacact accgcttctt cagttttggt gacgccatgt ttatttattg agaaagaatt
     1141 tctctaaatc ttctaatacc aataaatcgc taagatatta tttcaaagaa catctacaat
     1201 tgaaactcta gctagctgta gaagaggcct agtacattga aattaaaatg cttcccccta
     1261 gcttcgaaaa tattgccata gattgcgttg actctccaaa ttgattcatc tatattttat
     1321 ttcagcttcc tatattttct tcgctgtttg taaatcaaaa tgcaagacac atgagtagca
     1381 ccatatttgt tactcttatc tgtcctctca agagactatt atgagttatt tcagaatcat
     1441 tcactacttt gaccctgact ctccttagtc tcaaaatcaa agacttatac tcttcaaaaa
     1501 tctcttcaaa ccgcgtcaac gtcaccttgg attatatatg tgactgactt cgtcagttct
     1561 atctacaacc tcaaagcagt actttgagca acctgcgact agttttctag tttgctcttt
     1621 gattttcatt gagtattaaa caaaaagtga acaaatctga attctaatgt acagaagact
     1681 aggcttgttc acttttttat agtcgctata agatgacctt atctatagct tttt
//
LOCUS       DAVHIU010000181         1728 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVHIU010000181 DAVHIU010000000
VERSION     DAVHIU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.183"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            33..484
                     /locus_tag="VVN06_002003"
                     /pseudo
     CDS             33..484
                     /locus_tag="VVN06_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            649..948
                     /locus_tag="VVN06_002004"
     CDS             649..948
                     /locus_tag="VVN06_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU5563479.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1057..>1728)
                     /locus_tag="VVN06_002005"
     CDS             complement(1057..>1728)
                     /locus_tag="VVN06_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5563480.1"
                     /translation="RIYLELRNRAYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDV
                     GKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCS
                     PNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQD
                     NGMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYR
                     TKSFG"
BASE COUNT          559 a          338 c          302 g          529 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agtgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggaaaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca actcctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatcaaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaatc acgtaagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct tttattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcagc tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aaggcctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttcaata ataaaacgca tagtagcaag
     1021 agttttgtac tgaccccaaa aagttagaca attaatttat ccgaaggatt tagttctgta
     1081 ttgcacaggg ctaagtcctt ttagttttac cttaattcgt ttattgttgt agtaatcaat
     1141 atagtctaca atggcttgtt ccaattgctt aagcgactga aacgacttct cataaccgta
     1201 aaacatttcc gatttcagaa tcccaaagaa ggactccatc ataccgttgt cttggctgtt
     1261 tcccttacgt gacatggatg cttgaattcc cttaccctca agaaactgat gataagaatc
     1321 gtgttggtat tgccagcctt ggtcactatg gagaatcgta ttttcgtagt gcttctctgt
     1381 gaatgcctgt tccaacattg tttgtacttg ttctaaatta ggcgaacaag aaagattaaa
     1441 agcaataatt tcgctgttaa agccatctaa aactggtgat aagtaaagct tttgagtact
     1501 tgctggaatg gcaaattctg tcacatctgt atagcacttt tccattgttt tagagccttc
     1561 aaattggcct tgaatgagat tctctgcctt cttgccaacg tctcctttat gagaagaata
     1621 ttttcgtttc tgtcgcattt tagcttgtaa attgagtact ttcatcaagc cttgaactct
     1681 tttatgattt accagataag cacgatttct tagttctaaa taaatccg
//
LOCUS       DAVHIU010000182         1637 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVHIU010000182 DAVHIU010000000
VERSION     DAVHIU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.184"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..754
                     /locus_tag="VVN06_002006"
     CDS             <1..754
                     /locus_tag="VVN06_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5563481.1"
                     /translation="DYIDTLCKKHNINYIINYGTLIGAVRHEGFIPWDDDIDLSMPRE
                     DYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKIIDTRNTKTYESGIFIDI
                     FPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKDWIRTAFWLLLRPVSPRY
                     FANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKTIDLPFENLSLPAPEKFD
                     TILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            756..1565
                     /locus_tag="VVN06_002007"
     CDS             756..1565
                     /locus_tag="VVN06_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU5563482.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHHRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          555 a          294 c          289 g          499 t
ORIGIN      
        1 ggattatatt gatactctct gtaaaaagca caatatcaac tatattatta actacggtac
       61 tctgattggg gcggttcgac atgagggctt tatcccttgg gacgacgata ttgatctgtc
      121 catgcctaga gaagactacc aacgatttat taacattttt caaaaggaaa aaagcaagta
      181 taagctccta tccttagaaa ctgataagaa ctactttaac aactttatca agataaccga
      241 cagtacgact aaaattattg atactcgaaa tacaaaaacc tatgagtctg gtatctttat
      301 cgatattttc cctatagatc gctttgatga tcctaaggtc attgatactt gttataaact
      361 ggaaagcttc aaactgctgt ctttcagtaa acataaaaat attgtctata aggatagcct
      421 tttaaaagat tggatacgaa cagccttttg gttgctcctt cgaccagttt ctcctcgtta
      481 ttttgcaaat aaaatcgaga aagaaattca aaaatatagt cgtgaaaatg ggcagtatat
      541 ggcttttatc ccttcaaaat ttaaggaaaa ggaagtcttc ccaagtggta cctttgataa
      601 aacaatcgat ttaccctttg agaatttaag ccttcctgca cctgaaaaat ttgatactat
      661 tttgacacaa ttttatggag attatatgac cctaccacca gaagaaaaac gcttctacag
      721 tcatgaattt cacgcttata aattggagga ttaggatgca atatttagaa aaaaaagaaa
      781 ttaaagaaat tcaactagcc ctgctggact atattgatga gacttgtaag aaacatgata
      841 ttccttattt tctcagttat ggaaccatgc ttggagccat ccgccacaaa ggtatgattc
      901 cttgggatga tgatattgat atttcccttt atcgtgagga ttatgagcgt ttactgaaga
      961 ttattgaaga agaaaatcac catcgctaca aggttctttc ctacgataca tcttcttggt
     1021 acttccataa tttcgcatcg attttggaca cttctactgt tatagaagac catgttaagt
     1081 acaagcgtca tgataccagc cttttcatcg atgtcttccc aattgatcga tttacagact
     1141 tgagcattgt cgacaagagc tataagtatg tggcccttcg tcaactagct tatatcaaaa
     1201 aatcacgagc agttcacggt gatagcaaac taaaagattt tcttagatta tgtagctggt
     1261 acgctctccg atttgtcaat cctcgctact tttacaagaa aattgatcaa ctagtcaaaa
     1321 atgctgtaac caacactcct caatatgaag gaggagttgg gatcggtaag gaagggatga
     1381 aagaaatctt cccagttgat acctttaaag aactgatttt aactgagttt gagggccgta
     1441 tgttgcctgt tcccaaaaaa tatgaccaat ttttaaccca gatgtatggc gattatatga
     1501 caccaccatc aaaagaaatg caagagtggt atagtcatag cattaaagct tatcgcaaaa
     1561 actgattgag ggggattata caaactacta agatagaggt tattcaaaaa cataatttta
     1621 gtagaaaatg aaataca
//
LOCUS       DAVHIU010000183         1627 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVHIU010000183 DAVHIU010000000
VERSION     DAVHIU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.185"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..746)
                     /locus_tag="VVN06_002008"
     CDS             complement(<1..746)
                     /locus_tag="VVN06_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU5563483.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKV"
     gene            868..1614
                     /locus_tag="VVN06_002009"
     CDS             868..1614
                     /locus_tag="VVN06_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5563484.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
BASE COUNT          571 a          208 c          301 g          547 t
ORIGIN      
        1 aatacctttt gataatcaat aagatcttca ggatgaagtg cgttgattcc atccatttca
       61 tatttccgat ttaataaacg atatttgttt tcaccaaatt gatgaaaagg gagtagttga
      121 acttggtcga tatttaatga gttaaataga gtagcgaatt tttctgcatc ctctaaacta
      181 ttgttaaaat taggaataac tgggattctt aaaacgatag ttttattttg agaaaaagca
      241 taatgaatgt ttttaataat catttgatta aaaaccccag tcactttttt atgttttata
      301 gaattataat gttttaggtc tgtgtagata aaatccacat attgaattaa atcgataaat
      361 ttttcatgat caacaaaggc agtagtttca atggcagtgt gtatgtgatg ttctttagct
      421 gattttaaga tggctttagc aaattcaaac tgagcaaata tttcacctcc tgataaagtt
      481 aaacctccac cggattcttc gtaaaattct ttgtctttta atacctctgt aataatttct
      541 tctacagtct tttcttcacc gactaaggtg aatttctctc gttgagcatc tttcattttt
      601 tcaggtttca ttctttgaga ttcaggatta gaacaccatg gacagcgcag aggacatcct
      661 tttaaaaaaa cagttgtacg aatacccggg ccgtcatgaa ttgaaaagtg ttgaatatta
      721 aaaataattc ctttagatat ttccataaga gttcctcctc ttcataagtc tatcctatca
      781 caaaaacgaa agaaaaacaa ttacgaacga aaggttaatt tgttgttttt tgctttttat
      841 tttggtagaa taaaacgggg gtgtaaaatg aaaagattgg aacaaattat taaattagta
      901 tcagaatatg aaaagatcga cgttaataca ttatcggaaa aattaaatgt atcgaaagta
      961 acgattagaa aagatttaga taaattagag tcaaaaggtt tattacacag agagcatgga
     1021 tatgctgtat taaatagtgg agatgactta aatgtgcgtt tgtcaattaa ttatgaaatt
     1081 aagagaaaaa ttgttcagga agcagtaaaa ttggtgtcag ataatgaaac aataatgata
     1141 gaatctggat cgacctgtgc tttacttgct gaggaaattt gcaagcaaaa aagaaatgtt
     1201 acgattgtaa caaattcatt ttttatagca aattttgtga gagcttatga ttcatgtcgt
     1261 attattgttc ttggtggaga gtttcagaag gattcacagg ttactgtggg acctttatta
     1321 aaagaaatga tacagacttt tcatgtgcat caagcttttg ttgggacaga tggttacgat
     1381 aaagagatgg gctttacagg aaaagattta atgcgcagtg aggtagttca atatatttca
     1441 gcagcatcgg ataaagtcat tgtactaact gactcaagta aatttgataa aaggggtaca
     1501 gtaagaagat ttgctttaag tcaagtctat gaagtaataa cagacgaaaa actttctaaa
     1561 caaaatatag ctacattaga aaatgctggg ataatggtta aggtagtttc gtaagaggtt
     1621 aagtgta
//
LOCUS       DAVHIU010000184         1622 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVHIU010000184 DAVHIU010000000
VERSION     DAVHIU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.186"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(117..1253)
                     /gene="lctO"
                     /locus_tag="VVN06_002010"
     CDS             complement(117..1253)
                     /gene="lctO"
                     /locus_tag="VVN06_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU5563485.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT          468 a          372 c          299 g          483 t
ORIGIN      
        1 actgtcctga ttgatttgtc ctattcttat ttcattttat tatagttttc gatagcgatt
       61 tattcttcca atacacgaag aaaaacctcc acattcagtg gaggcaatct gttttatcaa
      121 tacaatttta agtcacgagg gtcaactggg aaggttgggt tgtatggatt gtgacggagc
      181 ttaaagtgtt tgacatcttc aatggtctga gctccagaca attgcataac tgtcttcaat
      241 tccgcattca agtgttcaaa gacttgacgc acaccgacac taccaccgag agccaagcca
      301 taaataacag ggcgaccaat agctaccaag tctgctcctg aagccaaggc tttaaagacg
      361 tgttgaccac gacgaacacc agagtcaaag acaatcggca cacgtctatc aactgcttct
      421 gccacttctt gaagcgagtc aaaggcagct ggtccaccgt cgatttgacg accaccgtgg
      481 ttggttaccc agataccaga agctcctgca gcaagtgaac gttcaacgtc ctcacggcat
      541 tgtggtccct tgacatacac aggaagacca gagtattcag cgataaattc tacatcgcgt
      601 ggagacaagc gttgtttagc tgatttgtaa acaaagtcca ttgatttacc agcaccttct
      661 ggcaggtatt cttcaacaat cggcatgcca actgggaaga caaaaccatt acgcttatcc
      721 acttcacgat tcccccctac agtagcatct gccgtcaaga caatcgcttt ataaccttca
      781 gccttcacac ggtccatgat gtggcggttg ataccgtcat ccttactaaa gtaaaattgg
      841 aaccaatggg gtgtcccttg aagggcttca gaaatctctg gaaggtcaac agtagagtaa
      901 gagctggttg tataaagaga accaaactca tgcacaccac gcgcagtcgc cacttccccc
      961 tgttcatttg ccaatttatg agccgcaaca ggcgccataa tgattggtga agatagtttt
     1021 tcacctgcaa attcaatctc tgtacttgga ttttcaacat cgcaaagtgt atgaggaacg
     1081 atgagtttgt ggttaaaggc gcggatattc tctcttaaag tgaaagtatc ttccgcccca
     1141 ctagcgatat agccaaatgc tgctttagga ataacttgtt gcgccattgg ctccaaatca
     1201 taggtattga tgaagtctac atgaccttct gcattgcttg ttttgtatga cataaaatgc
     1261 cctccttgat taagtaagcg tttgcttttt actacacaac tataataact cttttttcaa
     1321 tcattttcaa gagttttgct taaaaaccat cttatttttt catacaatat actagcccta
     1381 ctttccttaa actctaaact ttagtctata ttaagtgaat cgttgagatt ttttgctgcc
     1441 aggcatttga actacttttt tatttgctga ttctatctac taatactata aaaaaacaag
     1501 tgaataagac accagaacat cctctaatag gacctatgga cttatatcag tcccatatat
     1561 actgtttaag caagatacta tcctagttga cgctaacttc ctatcccact tattaatttt
     1621 tt
//
LOCUS       DAVHIU010000185         1614 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVHIU010000185 DAVHIU010000000
VERSION     DAVHIU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.187"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..392
                     /locus_tag="VVN06_002011"
     CDS             <1..392
                     /locus_tag="VVN06_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5563486.1"
                     /translation="DNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISSKMA
                     GKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYADSP
                     FILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            389..>1614
                     /gene="tilS"
                     /locus_tag="VVN06_002012"
     CDS             389..>1614
                     /gene="tilS"
                     /locus_tag="VVN06_002012"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5563487.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKT"
BASE COUNT          537 a          244 c          313 g          520 t
ORIGIN      
        1 ctgataatgt agctcataat ctattgggat attacatttc aaaccaatct gatgccacat
       61 tcaaatccaa aatgtctgcc attatgggag atgattggga tccaaaagaa aaattgattt
      121 cttctaagat ggccgggaag tttatggaag ctatttataa tcaaaatgga tttgtgctag
      181 agtctttgac taaaacagat tttgatagtc agcgaattgc caaaggtgtt tctgttaaag
      241 tagctcataa aattggagat gcggatgaat ttaagcatga tacgggtgtt gtctatgcag
      301 attctccatt tattctttct attttcacta agaattctga ttatgatacg atttctaaga
      361 tagccaagga tgtttatgag gttctaaaat gagggaacca gattttttaa atcattttct
      421 caagaaggga tatttcaaaa agcatgctaa ggcggttcta gctctttctg gtggattaga
      481 ttctatgttt ctatttaagg tattgtctac ttatcaaaaa gagttagaga ttgaattgat
      541 tctggctcat gtgaatcata agcagagaat tgaatcagat tgggaagaaa aggaattaag
      601 gaagttggct gctgaagcag agcttcctat ttatatcagc aatttttcag gagaattttc
      661 agaagcgcgt gcacgaaatt ttcgttatga tttttttcaa gaggttatga aaaagacagg
      721 tgcgacagct ttagtcactg cccaccatgc tgatgatcag gtggaaacga ttttgatgcg
      781 cttgattcga ggaactcgct tgcgctatct atcaggaatt aaggagaagc aagtagtcgg
      841 agagatagaa atcattcgtc ccttcttgca ttttcagaaa aaagactttc catcaatttt
      901 tcactttgaa gatacatcaa atcaggagaa tcattatttt cgaaatcgta ttcgaaactc
      961 ttacttacca gaattggaaa aagaaaatcc tcgatttagg gatgcaatct taggcattgg
     1021 caatgaaatt ttagattatg atttggcaat agctgaatta tctaacaata ttaatgtgga
     1081 agatttacag cagttatttt cttactctga gtctacacaa agagttttac ttcaaactta
     1141 tctgaatcgt tttccagatt tgaatcttac aaaagctcag tttgctgaag ttcagcagat
     1201 tttaaaatct aaaagccagt atcgtcatcc gattaaaaat ggctatgaat tgataaaaga
     1261 gtaccaacag tttcagattt gtaaaatcag tccgcaggct gatgaaaagg aagatgaact
     1321 tgtgttacac tatcaaaatc aggtagctta tcaaggatat ttattttcct ttggacttcc
     1381 attagaaggt gaatcaattc aacaaatacc tgtttcacgt gaaacatcca tacacattcg
     1441 tcatcgaaaa acaggagatg ttttgattca aaatgggcat agaaaaaaac tcagacgttt
     1501 atttattgat ttgaaaatcc ctatggaaaa gagaaactct gctcttatta ttgagcaatt
     1561 tggtgaaatt gtctcaattt tgggaattgc gaccaataat ttgagtaaaa aaac
//
LOCUS       DAVHIU010000186         1614 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVHIU010000186 DAVHIU010000000
VERSION     DAVHIU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.188"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            290..1162
                     /locus_tag="VVN06_002013"
     CDS             290..1162
                     /locus_tag="VVN06_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU5563488.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1314..1571)
                     /locus_tag="VVN06_002014"
                     /pseudo
     CDS             complement(1314..1571)
                     /locus_tag="VVN06_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          529 a          287 c          316 g          482 t
ORIGIN      
        1 ttttcgcctc taacaatcgc aatctcttct ttatctacga acgaaatcgc tttctatttt
       61 tgtaagtaaa gcatcacacg aaatccacga aaatgaaaac ctttgttgtg ttttcgtaaa
      121 aaatttgttg acagagcacg aaacgcctac tataattaaa tcaagtttaa ggaaataaca
      181 aaaaaacaaa tcggagggaa agacgatgaa cacattaaat gagaaagtaa tcaatatctg
      241 taaagcagta gttaaagaaa ctttaatcca agacatttag gagaaagaaa tgaagaaatc
      301 aagaaaatta gccacattag gaatctgttc agccttgttt ttaggcttgg ctgcttgtca
      361 acaacaacat gctacttctg agggtacgaa tcaaaggcaa agcagttcag cgaaagttcc
      421 atggaaagct tcatacacca acctaaacaa ccaggtaagt acagaagagg tcaaatctct
      481 cttatcagct cacttggatc caaatagtgt tgatgcattt tttaatctcg ttaatgacta
      541 taataccatt gtcggctcaa ctggcttatc aggagatttc acttccttta ctcacaccga
      601 atacgatgtt gagaaaatca gtcatctctg gaatcaaaag aagggcgatt tcgttgggac
      661 caactgccgt atcaatagtt attgtctttt gaaaaattca gtcaccattc caaagcttga
      721 aaagaatgac cagttgcttt tcctagataa tgatgcgatt gataaaggaa aggtctttga
      781 ttcacaagat aaggaagagt ttgatattct attttcgaga gttccaactg aggcaactac
      841 agatgtcaag gttcacgctg aaaagatgga aacattcttc tcacaatttc aattcaatga
      901 aaaagctcga atgctgtctg tagtcttgca cgacaatctg gatggcgagt atctgtttgt
      961 aggccacgtt ggggtcttag tacctgctga tgacggtttc ttatttgtag agaaattgac
     1021 tttcgaagag ccctaccaag cgattaaatt tgctagtaag gaagattgct acaagtattt
     1081 gggcaccaag tatgcggatt atacaggcga gggactggct aagcctttta tcatggataa
     1141 tgataagtgg gttaaacttt aatagtgtta tgatatgaca gtaatcttag gatttttatt
     1201 tctatttttg ataagttggt gggttatttc aatcgttaat agccagaaac cacgcaaaaa
     1261 tgaaacattt attggctatg tccaaagata cgatattgat gggaatggct atcaatcatt
     1321 accgtgtcct cagaactgag aggagttctt gaaatcgtaa aaccactttg aacaagagtt
     1381 acttcaaccc attggctccg acggattaag ttgctttcgt gaataccaaa atcagccgca
     1441 atttgttcat aagtgcggta ttctcgcaca tattgaagag tggccataag aagatcttct
     1501 aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat
     1561 acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa caagacgttt aaat
//
LOCUS       DAVHIU010000187         1608 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVHIU010000187 DAVHIU010000000
VERSION     DAVHIU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.189"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            279..>1608
                     /gene="dnaA"
                     /locus_tag="VVN06_002015"
     CDS             279..>1608
                     /gene="dnaA"
                     /locus_tag="VVN06_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5563489.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRL"
BASE COUNT          549 a          280 c          289 g          490 t
ORIGIN      
        1 ggaaaaatca ttatttcttt ttcaaatcaa gaagaatata gtagaattat caacagcctg
       61 aaataaggct gttcttttat ttttttatct cacaaggtta tccactatgt ttttcgataa
      121 aaagcttaat aaatcaataa tttcttcttt tatccccaac ctgtggataa agtttggtaa
      181 cattgtggat tatttttcac agcttgtgga aaattcttgc tatctatggt aaaatatctc
      241 tagtattaaa cttttaaata gtaaaggagg agaaaggatt gaaagaaaaa caattttgga
      301 atcgtatatt agaatttgca caagaaagac tgactcgatc catgtatgat ttctatgcta
      361 ttcaagctga actcatcaag gtagaggaaa atgttgccac tatatttcta cctcgctctg
      421 aaatggaaat ggtctgggaa aaacaactaa aagatattat tgtagtagct ggttttgaaa
      481 tttatgacgc tgaaataact ccccactata ttttcaccaa acctcaagat acgactagct
      541 cacaagttga agaagctaca aatttaactc tttatgacta tagtccaaag ttagtatcta
      601 ttccttattc agatacggga ttaaaagaaa agtatacctt tgataacttt attcaagggg
      661 atggaaatgt ttgggctgta tcagccgctt tagctgtctc tgaagatttg gctctgacct
      721 ataaccctct ttttatctat ggaggaccag gccttggtaa gactcactta ttaaacgcta
      781 ttggaaatga aattctaaaa aatattccta atgcgcgtgt taaatatatc cctgccgaaa
      841 gctttattaa tgactttctt gatcacctaa gacttgggga aatggaaaag tttaaaaaga
      901 cctatcgtag tcttgatctt ttgttaatcg atgatataca gtcactcagc ggaaaaaaag
      961 tcgcaactca ggaagaattt ttcaatacct ttaacgccct tcatgacaag caaaaacaga
     1021 ttgtcctaac gagtgatcgt agtccaaaac atctagaagg gctcgaggag aggcttgtca
     1081 cgcgttttag ttggggattg acacaaacta tcacaccccc tgactttgaa acacgtattg
     1141 ccattttaca aagtaagacg gaacatttag gctacaattt ccaaagtgat actctagaat
     1201 acctagctgg gcaatttgat tcaaatgttc gagatcttga gggagccatc aacgacatca
     1261 ctttaattgc cagagtaaaa aaaatcaagg atatcactat tgatattgct gcagaagcca
     1321 ttagagcccg caaacaagat gttagccaaa tgctcgtcat cccaattgat aaaatccaaa
     1381 ctgaagttgg taacttttat ggtgttagta tcaaagaaat gaagggaagt agacgccttc
     1441 aaaatattgt tttggcccgt caagtagcca tgtatttatc tagagaacta acagataata
     1501 gtcttccaaa aattgggaag gaatttgggg gaaaagatca taccacagtc attcatgccc
     1561 atgccaaaat aaaatctttg attgatcaag acgataattt acgtttag
//
LOCUS       DAVHIU010000188         1593 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVHIU010000188 DAVHIU010000000
VERSION     DAVHIU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.190"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(541..1548)
                     /locus_tag="VVN06_002016"
     CDS             complement(541..1548)
                     /locus_tag="VVN06_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5563490.1"
                     /translation="MLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQSILSH
                     KFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFFNPNY
                     YGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAGAIIY
                     LFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMALFKQN
                     PFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRLMMDM
                     SQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLVSDMT
                     D"
BASE COUNT          581 a          337 c          268 g          407 t
ORIGIN      
        1 gaagtgtcaa agactacagt gaaaatagga aatttgacgc agaaactttg gagtttagga
       61 agacatacag taaaatgaaa tacggacgga acaatgtgat tttggaattc aaattaaatt
      121 ataacaatat tgtagaagta tcattctagt attcaagatt cagtttacta tgtcttttca
      181 caccaacctt attccgaatt caattacttt tgtgatttac atatatagat taagactatc
      241 ttttatactt taaaatttct cgctacctta tccactatat gctcctcgct atcacgtttc
      301 tattcatagc ctacgatttc actattgctt tctctgacaa ttcttatttc ctgcgtcaga
      361 cttaaaacga tctatcccca gaccatttta atccgctacc tcacgatagt caggcttggg
      421 gagcgctatt gtattcaccg gtagtggatc cctacagagg acttacacct cagatgcacg
      481 acatgcccat cgtataaaaa aatctcctac ccaaggtaga agatttcaaa cttataaaac
      541 ttaatccgtc atgtccgata ccaacattcg atgctccaat ggaatactgc acataactag
      601 caagaaaata aagcctgact gaatccagaa gagagccaag tcaaaaattc cgtgcacagc
      661 aaccactgta aggaaagata gataaaggcc gataatcgga cgtttccccg actcctgact
      721 catatccatc atcaagcgaa caggagcaac agaagacaaa actaataaaa tggtacccac
      781 aattccgtaa ctcagaatcg tatcaatata aagactgtgg gcatgttcat gataaggagc
      841 atgtatccga ggataagagt gcatataggt caatggccct tcaccccaaa aaggattttg
      901 cttaaacaag gccatcccag catcccagat agaaatgcgt tcttccatag aagagtctaa
      961 agtacccatt cgaactccca aatcactaga aaagaggaaa ctcaaaccaa tcgcgaagac
     1021 cccaatacta agccaaaagg ccttccagtt tttaatagtc gtaaagagat agataattgc
     1081 tccagcgata atagcaggaa aggcagttcg attttgagta aagttcaaac caaagagatt
     1141 aacaaagcct gcaatcacac agaatacttt caaccaattc aacttggtcg ttgtaaacag
     1201 atagaaagca atcataatac agaaacaaca aataattcca taataattag gattaaagaa
     1261 ggtcacttct gcccggttct gatgccacac ctgcatattg ggtgaaagaa aagcatagtt
     1321 aaatttcttc acaatttgga aatgttctaa actggcaaaa gcagctgaca agacactacc
     1381 aaacaagacg aactgcaaaa tcaatcgaaa gaatttatgg gataaaatcg actgatagtg
     1441 caaaaagaaa atagtaaata gaaacattcc tactgaagcc acaagaccca tccaattttg
     1501 tgcaagaatg gatataacag tactatagct aagaaaaaga agcagcatcg gatgctcccc
     1561 cattttctga agaatacttt tcatgtctcc tgt
//
LOCUS       DAVHIU010000189         1552 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVHIU010000189 DAVHIU010000000
VERSION     DAVHIU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.191"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(141..1487)
                     /gene="celB"
                     /locus_tag="VVN06_002017"
     CDS             complement(141..1487)
                     /gene="celB"
                     /locus_tag="VVN06_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU5563491.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
BASE COUNT          566 a          317 c          250 g          419 t
ORIGIN      
        1 ctattttgct tcatacccta catagcgaaa aaatatgaaa aagcagagaa gaatatctta
       61 aaaagacctc ttcactgcta atgttaacgc tcattattta aactatatgg attctatcat
      121 cgagtatact tttttactta ttagatacct tgctcttctt tcaccaattt ttgatcatat
      181 acacggatga atggaagata gactaggaat gctgcaaatg cacatactag agcaactaat
      241 acagctcgaa gatctgctgt ccctaagaaa gctccaatcc ctactggagt tggccatgga
      301 acctgtgcga taattggctt aataaagttt agagaattcg ctacgtaata aatagtagca
      361 gtaaccattg gtgctaaaat aaatggtata gctaaggctg gattatagat aataggtaat
      421 ccaaaaatta atggttcatt aatattaaat aaggccggaa ctacagatgc tcgtcctatt
      481 gctttaagct gttcagattt agaggcaaaa gcaatatata aacatagtcc taaagttgca
      541 ccagaaccac ctgcaattac aaacatatta gaaaattcac ctgcaacagc gaagtgcccg
      601 ccagcagcat tttcagccat gttagcaaga gcaattggac taacaaatgc aaaaacaatg
      661 ttcgcaccgt ggatacctac aatccaaagt agttgagtca atagataaat aatcattaaa
      721 ccaatccacg aattagtcag attggataca aaaccaaatg gaattgcaat gactttaaaa
      781 atatccgttc ccattgctac aagaagaccg ttgataaaga taacaacaaa tgcaacaaca
      841 aatcccggaa ccaaagcggt aaatccacga gaaactcctt ctggaacagc ttcaggcatt
      901 ttaataaccc aattatgttt aacacacata cgataaataa gaacagtcac aattgccata
      961 atgattgcgg taaaaatccc tgttgtccca aaacgtgcga ctccatttcc cattgcccat
     1021 ccatctgcaa ttactgcacc ttcttttaga cttgtcacag tcttcatcat tccaccatca
     1081 aaaatgattt gcggtactgt catgacaaaa gccatcaagg caagcaaggc accattaaga
     1141 ggattcatat tgagttcttc ttcctctgca taaatttttg tcaattcata tgcaagtgat
     1201 agaacgaaat aaagagatag agaacccata gtcgcatagt ttgcaaccat gtaaagtgat
     1261 gtgaatttat caaatgaagc agagaaaata tctgccacaa ttggccaaaa tgagaaagct
     1321 tgtggcaaaa tactgaatac caaaaacatt gatcctacaa tagtaaatgg tacagcagcc
     1381 atacctgcag ccgtgatagc acgtactact ttaaactgag caagtttgcc cattggtccc
     1441 ataacatggt tttcaagaaa accaaacaac ccgttttgtt gatccataaa tagacctcct
     1501 taataaaaca taataatttt tactttctaa agactggttt caaatacaaa tt
//
LOCUS       DAVHIU010000190         1551 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVHIU010000190 DAVHIU010000000
VERSION     DAVHIU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.192"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(84..887)
                     /locus_tag="VVN06_002018"
     CDS             complement(84..887)
                     /locus_tag="VVN06_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU5563492.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLFSEKMYLDLDLDFS"
BASE COUNT          505 a          286 c          254 g          506 t
ORIGIN      
        1 ctaaaacttt gatgatatac gttttcttgt gagaatattt acttcatttt tgcctaaaat
       61 tcaatgttta ctcagtattt ggattatgaa aaatcgaggt ctaaatctag atacattttt
      121 tctgaaaaca aatcattttg accaccgagc aagagatttt caaaaaaagc tgttaaaaac
      181 tcagaacgtc gctgtaaaat ctttgcatta tctaatacca aggcatcacg aaaatatttg
      241 gaatgttgct gaaatggtgt attatcaata tcaaaaccaa actcacgaag atactgaatc
      301 aaaaagaccg ttactgtccg agtgtttcct tcgcgaaatg gatgaatctg ccagattcct
      361 gaaataaaat gctggatttg tttaaccaca tccgcctgag ttagtgtcgc atatgcaact
      421 tgtttttcct gattaaaatc ataatctaag gtcatttgaa tcatggagta atcagagtac
      481 acaacacttt caccattcaa aacaggttca ttctttgtga tattggtctg acgaaattgc
      541 cccaccggaa tagagggttc aaatatatct tgaaacaact ccttatgaat agcaagtaag
      601 gtcgcaggac taaagctaaa gcctcttcga gacaatagtt ctacaatgcg tagagaaacc
      661 aagtctgcct cttccgtact tgcatcaata gtatggtgat aagccgttgc atcctcataa
      721 acctgctcat aagtcagttc tccccgggac tgtttctcag ccaaagattc catatacgct
      781 gatggcacta gattgtcaac tttctgcaga ccaaaaccta tccgccataa atcacgcttc
      841 gcttcataag acaagtttgg attgtcaatg ttgtaagttg attgcataga aatatccttt
      901 caattgtttt tctgtgtcat cggtaaattg tgaaataggt atggaattgg cgaaaattat
      961 aaaaatcctt cacaataggt tctatgaaat atatatactt catcttaata gtgagcaagc
     1021 taaacttagc atttcatgcc ctcatatggg atgttcttta actaaataat atgattatcg
     1081 agatatatct ggataaatga actaataagt ctgacgcgta gacttatcaa agtcattggc
     1141 atacaccact atgaactcgt tgatctgttc aaatcccaac acattacctg agaagaaagt
     1201 tgcaatgttg tttttggtgc gggtttgaat ttaaaaattt gttatgtagt acctaatcta
     1261 aggaattaga acaatgcctc taatttttct ttaatacact gaaacattga tgattctggc
     1321 tgtatttttg aaacagctct tctttgctcc tggaaaatat cttcagaagt tatattctct
     1381 attcctaacg ctacttgaat ttttttctaa aatattcttt tccgttgcca tctttagaaa
     1441 aatcataacc ttccctatct acgctgttac ataaattagc taaaaaaaac tatggggttg
     1501 ggaaaggaag ataagaaacg tatttagccc aataatctat aaagcttctt t
//
LOCUS       DAVHIU010000191         1525 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVHIU010000191 DAVHIU010000000
VERSION     DAVHIU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.193"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..295
                     /locus_tag="VVN06_002019"
     CDS             <1..295
                     /locus_tag="VVN06_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563493.1"
                     /translation="DKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQNSDDKRGVIA
                     SNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKLEISR"
     gene            351..1046
                     /locus_tag="VVN06_002020"
     CDS             351..1046
                     /locus_tag="VVN06_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563494.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1058..1474
                     /locus_tag="VVN06_002021"
     CDS             1058..1474
                     /locus_tag="VVN06_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563495.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT          471 a          235 c          372 g          447 t
ORIGIN      
        1 agataaactt actgattctg aatcgaaaaa atacggtgaa tatattgatt tgtacgaatc
       61 gtgtcgtcag aacaatttaa gtgttctact taactttctt caaaattcgg atgataaacg
      121 aggagttatt gctagtaact cagtgagaat tatgtttgaa tctttggata aagcagacca
      181 aattgcggcc ctgaatattt gtagaaacta tgtagcacga gaaaatgttt taaataaaga
      241 agatgcagaa aaattgttaa atgaaattga taaaaaactt gaaattagca gataggacaa
      301 ttcaaataga gttgagtaaa aagtttgaaa agtgaaacaa ggagaaagct atgccagtaa
      361 atgaatatgg tcagatgatt ggtgagtcaa tggaaggtta tacacccggt gcactgcctt
      421 ctattgattt cttagaaggg cgttatgcta ggatagaggc tctctcggta gaaaaacatg
      481 cggaggattt gctagctgtt tatggcccgg atactcctcg ggagatgtgg acctatcttt
      541 ttcaggagcc agtagcagat atgggggagc tggttagcct tttaaatcag atgttggctc
      601 gtaaggaccg tttttactat gcaatcatag acaaggcgac tggtaaggct ttgggaactt
      661 tttctctcat gcgcattgac cagaataacc gagtaataga agtcggagca gtcacttttt
      721 ctccagagct aaggggtaca cggataggaa ctgaggctca gtatctccta gctcgctata
      781 tttttgagga gctaaactat cgtcgctacg agtggaaatg cgatgcctta aatctgccat
      841 ccagacgagt tgcggagcgt ttgggatttg tctatgaagg aaccttccgc caggcggtgg
      901 tttataaggg gcgtacgaga gatacggatt ggttgtctat gattgatatg gactggcctc
      961 aagtcaaaga tcgtttggaa acatggctgc gtcctgaaaa ttttgataaa aatggacagc
     1021 agtacaagag cttgagagaa ctttaagagg tgttgagatg attactatta aaaagcaaga
     1081 aattgtcaag ctagaggatg ttttgcatct ctatcaggct gtcggttgga caaattatac
     1141 ccatcaacca gagatgctgg agcatgcctt atcgcattca ttagtaattt atctggcact
     1201 tgatggtgat gctgtggtgg gcttgattcg tttggttgga gatggattct catcagtttt
     1261 tgtacaggat ttgattgttt taccaatcta tcagcgtcaa gggattggta gtgccctaat
     1321 gaaagaggcc ttaaaagatt acaaagatgc ttatcaagtc cagctggtga cagaagagac
     1381 agaaaaaaac gtgggatttt atcgttctat gggctttgaa atcttatcca cctataattg
     1441 tatagggatg acttgggcga atcgagaaaa ataacaaaac ttgttttttc ttaagcaaaa
     1501 tttaaggatg gtctagtatt atata
//
LOCUS       DAVHIU010000192         1470 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVHIU010000192 DAVHIU010000000
VERSION     DAVHIU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.194"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..1409)
                     /gene="tacF"
                     /locus_tag="VVN06_002022"
     CDS             complement(<1..1409)
                     /gene="tacF"
                     /locus_tag="VVN06_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU5563496.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLL"
BASE COUNT          547 a          241 c          302 g          380 t
ORIGIN      
        1 agtagactaa tataagagat agctgatagc aagacaatca aaccagtatt aatcaaaaat
       61 ggcaaattaa tgaccatatc cacaggatac atgaaattaa tcaggaaata aattgctaca
      121 aaggaaagtg aaaagagaga gtatcgaaca gtatagctaa agatatgtcc caagtggatg
      181 agttgttttc tatggatgaa aatgatatag aaaacaagta gagaagtctc tgataacata
      241 gttgtcagta agtagtattc aggagccacg atatggttga aaaagaggag actattcaag
      301 cccaaattga gtagcccagc aaagactgta tagactgtga tacgtttttc atagccattg
      361 gtaaagagaa tttgggaacc aagaatggta tctaaggcca ggataatcgt acgaaaagcg
      421 aagagagagg tcaagatgcc gcctccgata tatttttcac taccgtaaag taggatggca
      481 tttggtccta aaaccatgag tccaaaactc agtggaatga taaagaagtt aaagattcga
      541 ctacctctat taaccagaga aacataggct tctttgtctc ctttccccag atagtaactg
      601 agacgaggca cactcactcc aattgcacct gttacaaccc cagctataac ggtcacaatt
      661 cgctgagcta tggtatagta actaacgttg acatcaatcc ctgttttaac gaggaagagg
      721 cgatctaaaa aagtgaagag catattggca ttggcaaaga ctaacatggc tgtcagaggg
      781 agaaagagtg gtttaaaatc acttaggtga attttaacaa gtttgatgtc tcttttaatc
      841 caaaaataac taatcaggta gttaatcagc gtcgataaac tcatcacaag tgtatagaca
      901 acaatatcgt gttcattttt aacaaataag aaaatagaga ccagcatcag gatacggatg
      961 aaggcagttt tgtaaaagag aaaactgtaa ttttccagag cttcattgac ccattcgatt
     1021 gaaaaaatct gggcaatgag ttgaatcccc ataacaaggt agaccttttt gacgattgga
     1081 ttatcagtaa agaagagagg ataggctagg atatagacag cagtggtcaa aatcgtacaa
     1141 gcgatgcaca aataaaaaag actagaaaag gttctgttaa gatctttttt gttatccttg
     1201 acattactga tagcccttaa accgtagtta tagacaccat aagttgcaaa gggcaagaaa
     1261 aatgacaaaa tagtgtcgac tgagttgaag taaccatagt cagttcggtc caagacacgc
     1321 gcgacatagg ttccagttag gatgggaaaa ataatattca agacacgaat tcccatgtaa
     1381 gatagagcat ttaattttat acttttcatt caatttacct cgtttttcat tatatcataa
     1441 agttagctaa taagaaatga agggcagtaa
//
LOCUS       DAVHIU010000193         1468 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVHIU010000193 DAVHIU010000000
VERSION     DAVHIU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.195"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..743
                     /locus_tag="VVN06_002023"
     CDS             <1..743
                     /locus_tag="VVN06_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5563497.1"
                     /translation="ENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLIS
                     GFYNVNKGSIKIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDD
                     VMRALKLAGCDLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEA
                     SASIDPDNEFELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKEL
                     MSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            727..>1468
                     /locus_tag="VVN06_002024"
     CDS             727..>1468
                     /locus_tag="VVN06_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563498.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMPAGNVKMGL
                     AVIIPSILNFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISS"
BASE COUNT          551 a          199 c          270 g          448 t
ORIGIN      
        1 ttgagaatgt tagctttgct tataatgata aagctgtcat tgaaaattta tcctttaatt
       61 tagaagaagg aaagtcctac gcacttgtcg gttcatctgg atcaggcaaa tcaacagtag
      121 caaaacttat atcaggtttt tacaatgtta ataaaggaag cataaagata ggcgggatag
      181 caataagtga atattctgac gaagccttaa ttaaagccat ttcctttgtt tttcaagatt
      241 caaaattatt caagaagagc atttatgata atgtagcttt agctaataaa gatgcgacga
      301 aagatgacgt tatgagagcc ttaaaattag caggatgcga tttaatatta gacaaattcc
      361 cagaaagaga aaatacaatc ataggctcaa aaggtgttta tttatccggt ggagaaaaac
      421 aaagaattgc aattgctaga gcaattttaa aggattccaa aattattatt atggatgaag
      481 catcagcatc tattgaccca gataacgagt ttgaattgca aaaagctttt aaaaatctta
      541 tgaaggataa aacagttatc atgattgcac acaggctatc tacaattaaa gaccttgatg
      601 aaattattgt catggatagt ggaaaaatta tagaaagagg gtctgacaaa gaattaatgt
      661 caaaagatac aaggtataag agcctgcaag agatgtttaa cagtgcgaat gaatggaggg
      721 tttcaaatga aagagtttta taaaaaaaga tttgctctta cagatggagg agcaagaaat
      781 ttaagtaaag caacactggc ttcatttttc gtttattgta taaacatgct tcctgccata
      841 ttacttatga tttttgctca ggaagttttg gaaaatatgg gcaaaagcaa tggcttttat
      901 atagtattct cagttttgac tttgatagca atgtatattt tgctttctat cgaatacgat
      961 aaattatata acacaaccta tcaagaaagt gcagatttaa gaataaggac agcggagaat
     1021 ttatcaaaat tacctctatc ttacttttct aaacatgaca tttccgacat ttcacaaaca
     1081 atcatggctg atattgaagg catagagcat gcaatgagcc actcaatacc aaaggtgggc
     1141 ggcatggtac tgtttttccc attaatatct gtaatgatgc cagcgggcaa tgtcaagatg
     1201 ggtttagctg taattattcc atctatttta aactttatat ttataccttt atctaaaaaa
     1261 tatcaggtta atggacagaa tagatattat gatgtcttaa gaaaaaactc agaaagcttt
     1321 caagaaaata tcgaaatgca aatggagatt aaagcatata atttatcgaa ggatattaaa
     1381 gatgacttat ataaaaaaat ggaagatagt gagagagtac acttaaaggc ggaagtaact
     1441 acaattttaa ctttgtctat atcttcaa
//
LOCUS       DAVHIU010000194         1467 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVHIU010000194 DAVHIU010000000
VERSION     DAVHIU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.196"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            106..354
                     /locus_tag="VVN06_002025"
     CDS             106..354
                     /locus_tag="VVN06_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU5563499.1"
                     /translation="MNTKMMSQFSVMDNEMLASIEGGTDWGTVGKGAVYGAGIGVAMC
                     AVGGLLTGGSTWAMTAGCAWAGAKLGGSFTAIADNLWP"
     gene            370..579
                     /locus_tag="VVN06_002026"
     CDS             370..579
                     /locus_tag="VVN06_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU5563500.1"
                     /translation="MFNYKIVDNQELSNISGGGLGGDVVVGALSGAFQAGQSCIAGGP
                     QAYLICATGGAIVGGILAYGLRPPK"
BASE COUNT          465 a          211 c          367 g          424 t
ORIGIN      
        1 cgtttcgatg acaattcaag atttggatga aaaatttaaa aaaaaaatga tatactaaac
       61 ttgtcaaagt tgcaacaaga caaaaataaa aaaggagtat ttgtcatgaa tacaaaaatg
      121 atgtcacaat tttctgttat ggataatgaa atgctggcta gcattgaggg aggaactgat
      181 tggggaacag taggaaaagg agctgtctat ggagcaggta tcggagttgc tatgtgtgcg
      241 gtaggaggac tccttactgg tggttccaca tgggctatga ctgctggctg tgcttgggct
      301 ggagctaaac ttggtggttc ttttactgca attgctgata atctttggcc atagaaggga
      361 atagaaatta tgtttaatta caaaatagta gataatcaag aattaagtaa tatttctgga
      421 ggtggattgg gaggagatgt agttgtagga gccttgtcgg gtgcatttca ggcaggacaa
      481 tcttgtattg ctggaggccc tcaagcttac ttgatttgcg caacaggggg cgctatagta
      541 ggtggcattt tagcttatgg attgagacca ccaaaatagt tttaaaaagg aatgagtgtt
      601 aatatgttaa atgaaactat gattcagctt gtactcattg caatttgttg tgtgtacatt
      661 atttttaaca ccaaggctga taaaaaccaa aagagaggat atcgtacagc tttgtacctt
      721 ttcgttatgg ctggtattat atcctatatt atgaattatc tgaactggtt agattttttc
      781 ttactgataa ctccgataat gtgcctattt aaatttgaag ataaatggag ttagcaacgt
      841 ttgacacttc aataggttat tagctatcct ttggctcatt gtcaactgta gtgagttgaa
      901 gaaaatctaa gatcgagaaa ggacgaattt cgtcctttct tttttgatgt acgacgggca
      961 tgtcgtatat ctgaggtgta agtcctcggt gggcacctgc taccggtgaa cccaatagcg
     1021 attcccaagc ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct
     1081 ctacgaacag gaacgtgata gtaaggcgta tatagcggat aagggggcct caaactctaa
     1141 agtccaaaaa ggtagtcgta acctatatgc gtaaatcacg agagtaaacg aggaaagatg
     1201 tacgacttat cccgtgaggt ctcatgagcg ctaaaagcgt agtaacaacg aatcatgaga
     1261 agtcagccga acccatagta gtgaggaaac ttctgtagtg gaagtggagc gaaggggtga
     1321 acaattgaaa gtagaattgt ctgttttgat tatttgtttg ataggatggt agtatgaaaa
     1381 tataaacggt gactattgtt cataaaaaaa aggacacacg aatagaacgc ttatgtagcc
     1441 tgagagagga gttagttaag aaatctt
//
LOCUS       DAVHIU010000195         1454 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVHIU010000195 DAVHIU010000000
VERSION     DAVHIU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.197"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            263..955
                     /locus_tag="VVN06_002027"
     CDS             263..955
                     /locus_tag="VVN06_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU5563501.1"
                     /translation="MNTSFVHAVDGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVIKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTRTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGSTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            1009..1398
                     /locus_tag="VVN06_002028"
     CDS             1009..1398
                     /locus_tag="VVN06_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU5563502.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          532 a          205 c          316 g          401 t
ORIGIN      
        1 catgtattta gataagatgt ctgcaatcct ttatatatga gattgattac gatatagtta
       61 aagtgttagt tcaataatca atatatcatt ctaagtctat ttgatatata aaatatattg
      121 tttaacgttt gatagaaaag atgctatact agaagtagga tgtaatggca aaatctaata
      181 ctaataagcg ctatgcatta cgtaaactcg gaaagatttt tggttctgcg gtaatcgggc
      241 taactttatt tttaggagga atctgaatac aagttttgtt catgctgttg atgggattca
      301 atatgtcaga gatgatacta gagataaaga agagggaata gagtatgatg acgctgacaa
      361 tggggatatt attgtaaaag tagcgactaa acctaaggta gtaatcaaga aaatttcaag
      421 tacgcgaatt cgttatgaaa aagatgaaac aaaagaccgt agtgaaaatc ctgttacaat
      481 tgatggagag gatggctatg taactaggac aaggacctac gatgttaatc cagagactgg
      541 tcatgttacc gaacaggtta ctgttgatag aaaagaagcc acggatacag ttatcaaagt
      601 tccagctaaa agcaaggttg aagaagttct tgttccatgt gctactaaat atgaagcaga
      661 caatgacctt tctgcaggac aggagcaaga gattactcta ggaaagaatg ggaaaacagt
      721 tacaacgata acttatgatg tagatggaaa gagtggacaa gtaactgaga gtactttaag
      781 tcaaaaagaa gactctcaaa caagagttgt taaaaaagga accaagcccc aagttcttgt
      841 ccaagaaatt ccaatcgaaa cagaatatct cgatggctca actcttgata aaagtcaaga
      901 agtagaagaa gtaggagaaa ttggtaaatt actcttacta caatctatac tgtagatgaa
      961 cgtgatggaa caattgaaga aactacttct cgtcaaatta ctaaagagat ggtaaaaaga
     1021 cgtataagga gagggacgag agaacctgaa aaagttgttg ttcctgagca atcatctatt
     1081 ccttcgtatc ctgtatctgt tacatctaac caaggaacag atgtagcagt agaaccagct
     1141 aaagcagttg ctccaacaac aggctggaaa caagaaaatg gtatgtggta tttttataat
     1201 actgatggtt ccatggcaac aggttgggta caagttaatg gttcatggta ctacctcaac
     1261 agcaacggtt ctatgaaagt caatcaatgg ttccaagttg gtggtaaatg gtattatgta
     1321 aatacatcgg gtgagttagc ggtcaataca agtatagatg gctatagagt caatgataat
     1381 ggtgaatggg tgcgttaaca gattcctccc tgcgacaaaa ttgataatag tatgtctgaa
     1441 tatataatta tata
//
LOCUS       DAVHIU010000196         1427 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVHIU010000196 DAVHIU010000000
VERSION     DAVHIU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.198"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            19..1365
                     /gene="gdhA"
                     /locus_tag="VVN06_002029"
     CDS             19..1365
                     /gene="gdhA"
                     /locus_tag="VVN06_002029"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU5563503.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          427 a          291 c          312 g          397 t
ORIGIN      
        1 caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt gtttgaaact
       61 gtgaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga atttttcaac
      121 actttagaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat cttggcacgt
      181 attactgagc ctgagcgcgt ggtttctttc cgtgttcctt gggttgaccg tgatggaaaa
      241 attcaagtaa accgtggtta ccgtgttcaa ttcaactcag ctgttggacc atacaaaggt
      301 ggacttcgtt tccacccaac tgtaaaccaa gggatcttga aattcctcgg atttgaacaa
      361 atctttaaaa atgtcttgac tggacttcct atcggtggag gtaaaggtgg atcagacttc
      421 aatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt catgactgaa
      481 ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg tgttggtgga
      541 cgtgagattg gttaccttta cggtcaatac aaacgtctta accaatttga tgctggtgtc
      601 ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc aactggttac
      661 ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt tgctggtaag
      721 aaagtcgtta tttcaggttc tggtaacgtt gctcaatatg ctcttcaaaa agcaactgaa
      781 ctcggtgcaa ctgttatttc tgtttctgac tcaaatggtt atgtcatcga tgaaaatggt
      841 atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gtgctcgttt gactgagtat
      901 gcagctgaaa aagcaactgc aacctaccac gaaggtactg tatggactta cgctggaaac
      961 tatgacattg ctcttccatg tgccactcaa aacgaaatca acggtgaagc agctaaacgt
     1021 ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag cgaccttgat
     1081 gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgcaaaagc tgccaacgct
     1141 ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct ctcatggact
     1201 cgtgaagaag ttgatggacg tctcaaagac atcatgacca acatctttaa cacagctaaa
     1261 acaacttcag aaacatatgg tcttgataaa gactaccttg caggagctaa cattgctgcc
     1321 tttgaaaatg tagcaaacgc tatgattgcc caaggcgtgg tataaaaaaa aattacggaa
     1381 actgactacc cagacgggta gccagttttt tcaattccta aaattta
//
LOCUS       DAVHIU010000197         1413 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVHIU010000197 DAVHIU010000000
VERSION     DAVHIU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.199"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(4..867)
                     /locus_tag="VVN06_002030"
     CDS             complement(4..867)
                     /locus_tag="VVN06_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU5563504.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
BASE COUNT          432 a          281 c          240 g          460 t
ORIGIN      
        1 taactaatct ataagttctt tatattgctg aaaacgcaat tcaaaaaggg atgttaattg
       61 tggattttct aatacctgca gagattggat aaagcgttca atctcttttt gattgcttcc
      121 ctttgtttga agaaagacac tcatcttctt taaaaattgc cacgatactt tttcaaaaac
      181 atcatacggt cgtaacatcc tctccaactc ggcttcgaag attgggatgt aggagaaaag
      241 ttttcgctcc atgagttctg ataagatatt taagagtcct tgcttcatat acaatcgatt
      301 gtgtactaac tctttaaatt ctttggattt ttcgagtaag gaggttgata aaaaaatcag
      361 atcttgattg ctcaagaagg gcatggtatt gcaaaagaga tagagttcaa accaggtcca
      421 agactcgata gcatagagat aggtggtcaa aaactcgcta tcctcctctg ctagtgggta
      481 gcttttattt agtgaatgga tggcatcttt aatcacgatg gcattcaaac gacgataggt
      541 ctgcgccatc tgttcttgat cgacttcctc caatagctgc tctaaagcag ctatatcctg
      601 atgggcaaag cgattcacaa cctttcgacc gattcgcata tgtggagatt cttgatagtt
      661 gttgagcttg tgcccaaact catcaaaggt cacatttata ccttggatag ctagaatcaa
      721 cttatccgca gacagcatag actgccctag ttcaaacttg gacaactgag aagctgttag
      781 accctcacaa gccacatctg tctgcttaag ctttctcgcc aaacgtaatt ccttgtaaaa
      841 ttcccccagt tccattctct caatcatctg accacctcct agcttttgca tattatatca
      901 ttatttacaa tcatttgtca aaatattctg atatttaaga gggcaaaaaa gccagcctta
      961 agctgactct aaaaggaccg agccttgtac aatacagaac tcaatcctcg ttctaaatag
     1021 aatgtctaac ttttttgggc agtacacgag cttggggaac agccactgga acgatgaggt
     1081 gtgagctcaa aatatcctcc agttatgttt ttcctaatag tataccggaa gagtgaaagg
     1141 attttataat ggagcggtta caaagaacct actttctatt aaacagtata ctatgaaaat
     1201 gtgaaaattt aacatttttt tgtacaaatt ttataaatta ttgccttttt aatatcaata
     1261 gttaatctct tatccagatc ccccttgtgt aaactttatc tttataagct tcaaggcccc
     1321 tatcccatct atttgcaaca attagatcac tttgttttgt aaatagttca aaattctttt
     1381 caataattac gttatctata ctaacgttta aat
//
LOCUS       DAVHIU010000198         1401 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVHIU010000198 DAVHIU010000000
VERSION     DAVHIU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.200"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            390..1391
                     /locus_tag="VVN06_002031"
     CDS             390..1391
                     /locus_tag="VVN06_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5563505.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
BASE COUNT          522 a          191 c          265 g          423 t
ORIGIN      
        1 catgatgttt attcgattag atgagtaacc aaaaataaca aaaaaacgct atttcgcgtt
       61 tgaactgtac ctcaaaagtt agacagaaaa aatctaattt ttggggtgtt tttgttaaga
      121 aaagaccttc aatcatttaa gcaaaatgta catctagatt tatagccctc taaaactctt
      181 gctttttttg tagaagatac gaaaaaagcc ataaaaccaa ggcttttcct gttttatgta
      241 gatgtttaca catgaaaatt gaagtatttt caaaagctaa ttggagtgat tttattcatg
      301 atgggataaa tgatctggcc aaaaatgatt gagaaatagt tgcttaaaaa gcatttgcat
      361 gatactatat attgaaagga ggtgataata tggtagaaca aagaaaatca attaccatga
      421 aagatgttgc tttagaagca ggagttagtg ttggaactgt ttcacgtgta attaataaag
      481 aaaaaggcat taaagaagta actttgaaaa aagtggaaca agcgattaaa actttgaatt
      541 acattccaga ttactacgct agaggaatga aaaaaaatcg aacagaaacg attgcaatca
      601 ttgtaccaag tatctggcat cccttctttt cagaatttgc tatgcatgtg gaaaatgaag
      661 tctataagag aaataacaaa ttactcttat gttctatcaa tggtacaaat agagagcaag
      721 actatctgga gatgttgcgt cataataaag ttgatggagt ggttgccatt acctatagtc
      781 caattgaaca ttacttgacg tcaggaattc cctttgttag tattgaccgc acatactcag
      841 atattgccat tccttgtgtt tcatcagata atgatgcagg tggaagagaa gcagctaaac
      901 aattaattag taaaggatgt cagcatttag cctttgtggg cggacataat acaaccatta
      961 atgaaaccaa acgtcgtaga atttcatttg aaaagtatgt ccaagagcat aaaatacctc
     1021 ttagtatttt tgatttggat gagacagtta ctgactatta tggaaagcta gagagtttct
     1081 tggaagaaaa ttcttctata gatggaattt ttacaatcaa cgattttaca gctttggatg
     1141 tgattgaaat actaaaaaaa aatggcaaac gtattcctca tgatgtccaa attattggat
     1201 acgatggaat taaaatggct ggagatagag attatctact ttcaactatt gaacaaccat
     1261 tggaagaaat ggcaaaagaa gcggttcgta ttttgtttga tataattgat ggaaagactg
     1321 ttaatttgca gacaatccta ccagtaaaat ttgttgaagg aaaaacaaca aaaaatgaaa
     1381 ataagtcttg acagaaagcg c
//
LOCUS       DAVHIU010000199         1363 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVHIU010000199 DAVHIU010000000
VERSION     DAVHIU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.201"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..1310
                     /gene="phtE"
                     /locus_tag="VVN06_002032"
     CDS             <1..1310
                     /gene="phtE"
                     /locus_tag="VVN06_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU5563506.1"
                     /translation="KNSTFNNQNFTLANGQKRVSFSFPPELEKKLGINMLVKLITPDG
                     KVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFKYTIASKDYPEVSYDGTFTVPTSLA
                     YKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDALVRVFDEFHGNAYLENNYKVGEIKL
                     PIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYIVEVPILEKENQTDKPSILPQFKRN
                     KAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTSNSTLEEVPTVDPVQEKVAKFAESY
                     GMKLENVLFNMDGTIELYLPSGEVIKKNMADFTGEAPQGNGENKPSENGKVSTGTVEN
                     QPTENKPADSLPEAPNEKPVKPENSTDNGMLNPEGNVGSDPMLDPALEEAPAVDPVQE
                     KLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVIKKNLSDLIA"
BASE COUNT          543 a          201 c          266 g          353 t
ORIGIN      
        1 taaaaaatag tacgtttaat aatcaaaact ttactctagc caatggtcaa aaacgcgttt
       61 cttttagttt tccgcctgaa ttggagaaaa aattaggtat caatatgcta gtaaaattaa
      121 taacaccaga tggaaaagta ttggagaaag tatctggtaa agtatttgga gaaggagtag
      181 ggaatattgc aaactttgaa ttagatcaac cttatttacc aggacaaaca tttaagtata
      241 ctatcgcttc aaaagattat ccagaagtaa gttatgatgg tacatttaca gttccaacct
      301 ctttagctta caaaatggcc agtcaaacga ttttctatcc tttccatgca ggggatactt
      361 atttaagagt gaaccctcaa tttgcagtgc ctaaaggaac tgatgcttta gtcagagtgt
      421 ttgatgaatt tcatggaaat gcttatttag aaaataacta taaagttggt gaaatcaaat
      481 taccgattcc gaaattaaac caaggaacaa ccagaacggc cggaaataaa attcctgtaa
      541 ccttcatggc aaatgcttat ttggacaatc aatcgactta tattgtggaa gtacctatct
      601 tggaaaaaga aaatcaaact gataaaccaa gtattctacc acaatttaaa aggaataaag
      661 cacaagaaaa ctcaaaactt gatgaaaagg tagaagaacc aaagactagt gagaaggtag
      721 aaaaagaaaa actttctgaa actgggaata gtactagtaa ttcaacgtta gaagaagttc
      781 ctacagtgga tcctgtacaa gaaaaagtag caaaatttgc tgaaagttat gggatgaagc
      841 tagaaaatgt cttgtttaat atggacggaa caattgaatt atatttacca tcgggagaag
      901 tcattaaaaa gaatatggca gattttacag gagaagcacc tcaaggaaat ggtgaaaata
      961 aaccatctga aaatggaaaa gtatctactg gaacagttga gaaccaacca acagaaaata
     1021 aaccagcaga ttctttacca gaggcaccaa acgaaaaacc tgtaaaacca gaaaactcaa
     1081 cggataatgg aatgttgaat ccagaaggga atgtggggag tgaccctatg ttagatccag
     1141 cattagagga agctccagca gtagatcctg tacaagaaaa attagaaaaa tttacagcta
     1201 gttacggatt aggcttagat agtgttatat tcaatatgga tggaacgatt gaattaagat
     1261 tgccaagtgg agaagtgata aaaaagaatt tatctgatct catagcgtaa ggaatagcag
     1321 tagaaaaagt ctgaatcaaa aatgaagttc tctcaaaagt tag
//
LOCUS       DAVHIU010000200         1331 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVHIU010000200 DAVHIU010000000
VERSION     DAVHIU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.202"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..829
                     /locus_tag="VVN06_002033"
     CDS             <1..829
                     /locus_tag="VVN06_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5563507.1"
                     /translation="KIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLKEEGK
                     FLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQGASL
                     STADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGVSTLE
                     NLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITSLSER
                     AVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIRYHS"
     gene            936..1211
                     /locus_tag="VVN06_002034"
     CDS             936..1211
                     /locus_tag="VVN06_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5563508.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          436 a          205 c          288 g          402 t
ORIGIN      
        1 taagattgta accgattcat ctgttactat tgaacctgaa ctagtaaagc aattagatat
       61 tacaattgtt ccattatctg taatgattga taatgttgtt tattctgatg cggatttgaa
      121 agaagaaggt aaatttcttc agttgatgca agaaagtaag aatcttccga aaacaagtca
      181 gccacctgta ggtgtctttg ctgagatttt tgaagaccta tgcaaagatg gtggccagat
      241 tcttgctatt catatgtccc atgctctttc gggtacggta gaagcagcac gccaaggtgc
      301 tagcctatct actgcagatg tgactgttgt tgatagttcc ttcactgacc aagccctgaa
      361 attccaagtt gttgaggctg cgaagttagc tcaagaaggt aaagatatgg aggcaatttt
      421 atgtcatgta gaagaggtta aaaaccatac agagctttat attggtgttt caactttgga
      481 aaatcttgtc aaaggtggac gaattagccg tgtaactggc ttgttgagct ctcttctcaa
      541 tatccgtgtt gtcatgcaaa tgaaagacca tgaattgcag ccaatggtta aaggtcgtgg
      601 aactaaaaca tttaaaaaat ggttagatga gttgataaca tcgctttctg aacgtgctgt
      661 agcagagatt ggaatttcat attctggtag tgatgattgg gcaaaagaga tgaaagaaag
      721 cttacaagct tatgttgaaa agccaatttc tgttttggaa acgggttcca ttattcaaac
      781 tcacacgggt gagaacgctt gggctatttt gatacgttac cactcctaaa aaaataaata
      841 aaatgggaag aaatagcgtt tttaacttga cctaaaaggg attttaggat atgattatat
      901 ttgttaatta gaaattattt ggaggaatca ttaacatggc aaacaaacaa gatttgatcg
      961 ctaaagtagc agaagctaca gaattgacta agaaagactc agcagcagca gttgaagctg
     1021 tatttgcagc agtagctgac tatcttgcag ctggtgaaaa agttcagttg atcggttttg
     1081 gtaactttga agttcgtgag cgcgcagaac gtaaaggtcg caacccacaa actggtaaag
     1141 aaatgacaat tgcagcttct aaagtaccag cattcaaagc tggtaaagct cttaaagacg
     1201 ctgttaaata atcagtcttt aaaaagccta ttgtatcaag ctttctagct tggtcagtag
     1261 gctttttaat tatgaaatct agtaataaat agagttaaaa agttgaatgt tccgaaaata
     1321 ttgtatatat a
//
LOCUS       DAVHIU010000201         1329 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVHIU010000201 DAVHIU010000000
VERSION     DAVHIU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.203"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            165..505
                     /gene="tnpB"
                     /locus_tag="VVN06_002035"
                     /pseudo
     CDS             165..505
                     /gene="tnpB"
                     /locus_tag="VVN06_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(538..1329)
                     /locus_tag="VVN06_002036"
                     /pseudo
     CDS             complement(538..1329)
                     /locus_tag="VVN06_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          399 a          272 c          244 g          414 t
ORIGIN      
        1 tttttatcat tttgaaatct actgctaatc aatgaagaag caaagatcaa actaggcagc
       61 tagttacaag ttacgtaagc gcgtcataac aaggtatcta tcctaattcc ccaacttcca
      121 gtatactaga taaaaaataa ttatcggagg aaaggtattg tactatgata attcaactaa
      181 gtgatttagg tcaagttcac cttgtttgta gcaagataga tatgaggcag ggaatagact
      241 cattagccta tgtagttaaa acccactttg aattggatcc tttctccggt caagtctttc
      301 tcttttgtgg tggacgtaaa gaccgcttta aagcccttta ctgggatggt caaggatttt
      361 ggctactata taaacgcttt gagaacggca gactgacttg gcccagtaca gaaaaggatg
      421 tcaaagctct cacacctgaa caagtagact ggcttatgaa gggcttttct atcactccaa
      481 aaataaatcc atcagaaagt cgtgatttct attgaaatga ggactttctt tttatactca
      541 tctgctttca aaaagcattc tagtccatct ccgattaacg atgggcttta tcacctcctt
      601 ctccagtcct tgtatgatat cttaaagttg attcatgaca tcttccaaag tttgaaaggc
      661 tttattttta aatccacgtt tacgaatctc tttccacact tgttcaatgg ggttcatctc
      721 tggtgtgtac ggaggaataa atgcaaagcc aatattagtc ggaatcttta aggtacttga
      781 tttatgccat atagcattgt ccataacgaa taaaagataa tcatctggat aagcttgtga
      841 aagctcttct aaaaagacgt tcatccactc agtattacat ccaccagcta ttaagaaaaa
      901 tgattcgcct gtatgggtcc ataacaatag cgaaattctc gtatatagtg actatggaca
      961 tgtggaccta ctcctattgg agaccaacaa gatcccagtt tactgattct accgaagcca
     1021 gcctcatctt ggtacatcaa gcgaacctta tgaaaacgtc tactggtttt aaaacgcttt
     1081 cttttcttct tgaattgaga ttttattttt agacgcgaca atggtttgag cgtctgcttt
     1141 cttaggatgt tctggacgtg gcataatatt tcgccaacca tggcgcttca acagttgata
     1201 gaaggcatca cgtgtgtagg aacgacctaa ctccttttta taagcctgaa ataaggcatc
     1261 aattgtaaca aattctcctg cctctgcagc cttcaaatga cgggcaagaa aggctttctc
     1321 ttccccaac
//
LOCUS       DAVHIU010000202         1286 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVHIU010000202 DAVHIU010000000
VERSION     DAVHIU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.204"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..800
                     /locus_tag="VVN06_002037"
                     /pseudo
     CDS             1..800
                     /locus_tag="VVN06_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(835..1044)
                     /locus_tag="VVN06_002038"
                     /pseudo
     CDS             complement(835..1044)
                     /locus_tag="VVN06_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1210..1283)
                     /locus_tag="VVN06_002039"
     tRNA            complement(1210..1283)
                     /locus_tag="VVN06_002039"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1247..1249),aa:Asn,seq:gtt)
BASE COUNT          422 a          267 c          207 g          390 t
ORIGIN      
        1 aaactagaat tcttcttaga aaaggccgtt ttaagtgcta tcattgctca aaaatggagg
       61 ttgctgagac ttctatcgtc aagaagaatc atcaaattcc ttgtatcatc aaccaaaaga
      121 ttgctcagaa gctaattgaa aagacttcta tgaccgacat tgatcatcag ttgtctattt
      181 caacttcaac tgtcattcgc aagatcaatg attttcactt tgagcatgat ttttcgcgtc
      241 ttcctgagat tatgtcctag gacgttgaaa cagtctgggg agtgactgtt tcaatcggga
      301 gatagagatg agctttattg ctcaggactt tgataagctc aatatcataa ctgttcttga
      361 gagcagaaca caagccatca tccgaaatcc catgaataca aggctatcaa gcgatactga
      421 aagctcattc aacaagatag tcagaaactg agtgataaac gattttatcg ccctactttt
      481 cgtatgcact tgactaataa agaaattcta gataaacttt tgagctattc cgaagacttg
      541 aaacaccact ataatctcta tcagctcttg cttttccacc tctttttcag actctgttta
      601 aaaccttcct caaagataaa gagaaaatcg tcaacgccct tcaattacct tattccaacg
      661 ccaaactgga agccaccaat aatctcatca aacttatcaa gcacaatgcc tttggtttta
      721 ggaactttga aaacttcaaa aaagaaagga cgaaatttgt cctttctaga tcttcgcttt
      781 cttcaaccca ctacagttga caaagaaccg aaaaatgtct cgcctctaat atagtcaatt
      841 gaaacaagaa caagacaaaa gagcctcgta aaaggtattg caacttggta ataccttttt
      901 gaggtgcttt ttgatatgag cccatgtttt ctcaatagga ttgtactcag gtgagtaggg
      961 aggaagatgt aaaagtttat gcccaaactc ttcgcataaa agttctagct tccccattct
     1021 atggaatctt gcattgtcca taatatagta gattgaaact agaatagtac acctctactt
     1081 ctaaaacatt gttagaattc gattttactg tcctaatcga tttgtcctat tcttatttca
     1141 ttttactata acacgaaata gattattatt acataacaaa aaagaggtct aaacctctta
     1201 actcaattac tccgccagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct
     1261 actaccaact gagctatggc ggatta
//
LOCUS       DAVHIU010000203         1266 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVHIU010000203 DAVHIU010000000
VERSION     DAVHIU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.205"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..>1266)
                     /locus_tag="VVN06_002040"
     CDS             complement(<1..>1266)
                     /locus_tag="VVN06_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU5563509.1"
                     /translation="QNEVFSIRKSKVGVCSVLLAVLFIGTQSVLANEVVGETSSSKDN
                     MQMNSLLSSTSTSLSAEPHSDTLSLDNNSSLSTDNNGGVSINQNKPDITNDTMMGNIS
                     GMEVNTILSTNLNEHVALNVKDYGAIGDGVNDDRQAIQDAIDAAAQGLGGGNVYFPEG
                     TYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIVFMTGLFTDDGAQVEWGPTEDI
                     SYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQI
                     AGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEPLTRKGFPYALNDDGKKSENVT
                     IQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKILNNHFDNMMYAGVRFTGFTDVL
                     IKGNRFDKKVKGESVHYRESGAALVNAYS"
BASE COUNT          388 a          257 c          176 g          445 t
ORIGIN      
        1 tagctataag catttactaa agctgctccg ctttctcgat aatgtacact ctctccttta
       61 actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca
      121 cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt
      181 gtcgacaatg tttgatagtg tgtgccaatt gctgttacta attccccaga tttatcactt
      241 ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag
      301 gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt
      361 tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat
      421 acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca
      481 ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt
      541 ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatagtacca
      601 ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc atccgtaaat
      661 aaacctgtca taaaaacaat agaagggtga ttcttaatat ttataccatt tagaatggta
      721 gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa
      781 taagttcctt ccggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt
      841 gcatcttgaa ttgcttgtcg atcatcatta accccgtcac ctattgctcc ataatcttta
      901 acattcaaag ctacgtgttc gttcagatta gtacttaaaa tagtattcac ttccatccca
      961 gagatgttac ccatcatagt atcatttgtt atatcaggtt tattttgatt aatactcact
     1021 cctccattat tatcagtact taaacttgaa ttgttatcaa gactgagagt atcagaatgg
     1081 ggttccgcag ataatgatgt agatgtgcta ctcaacaaag agttcatttg catattatct
     1141 ttagacgaag aagtttcacc aactacttca ttagctaaaa cactttgtgt tccaataaat
     1201 aaaaccgcta agagaactga gcaaactccc accttagact tacgaataga gaaaacctca
     1261 ttttgt
//
LOCUS       DAVHIU010000204         1194 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVHIU010000204 DAVHIU010000000
VERSION     DAVHIU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.206"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            126..230
                     /locus_tag="VVN06_002041"
                     /pseudo
     CDS             126..230
                     /locus_tag="VVN06_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037603748.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            250..825
                     /locus_tag="VVN06_002042"
     CDS             250..825
                     /locus_tag="VVN06_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU5563510.1"
                     /translation="MFVGFLVYVIVATVGYTINQGDYFQNEHLFIIAKTFLISLSVAY
                     AKWFDMISLRRLTKKEVLLFIASFLLCVLVNIGYHSLFTVSSGAGYQHLEAASTGISL
                     SFIASATVFGPILEEFVFRGILQGVVFENSWLGLVLTASLFSFLHAPYDFPSFIYYLF
                     GGFMLGFAYKKSQKLSVAILVYICYNCLSFL"
     gene            989..>1194
                     /locus_tag="VVN06_002043"
     CDS             989..>1194
                     /locus_tag="VVN06_002043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563511.1"
                     /translation="MGRGSFSKNYMVWLDNHVRILMAALNTSLTTNIKFGLVGTVGGR
                     FAGYGMYVLGYPGDYLDITNHRVVS"
BASE COUNT          357 a          182 c          234 g          421 t
ORIGIN      
        1 gagcgaaaaa tatcataatt ggtactttta caactaaata tattcgctct gttgtaaaat
       61 gaagtgcaac acaaaagaca tgttcatctg atatactaga gttgcgaaaa acagtataaa
      121 ggaagatgaa catgtccaca aactattcta ccacaaatca ataatacaat cacttatctg
      181 aagctgaaag aggagaaatt gaagcttatt taagcgtagg actcaaacct gtttaataaa
      241 tatcatgccg tgtttgttgg atttttagtc tatgtaatag tcgctacagt aggctatacc
      301 atcaatcaag gtgattattt tcaaaacgag cacctattta ttattgcaaa gactttcttg
      361 atttcactga gtgttgccta tgcgaaatgg tttgatatga tttcgctaag aaggttaacg
      421 aaaaaagaag ttctactttt tattgcaagc tttcttcttt gcgtgctggt aaatataggt
      481 tatcatagtc ttttcacagt ttcttctggt gcaggatatc aacaccttga ggctgctagt
      541 acaggaattt ccttatcctt tattgcaagc gcgacagttt ttggccctat tctggaggaa
      601 tttgttttca gaggaatcct tcaaggtgtg gtttttgaaa attcttggtt ggggcttgta
      661 ctgactgcct ctctcttctc tttcctgcat gctccttatg attttccttc gtttatctat
      721 tatctatttg gaggtttcat gctgggcttt gcttataaaa agagtcaaaa actatcggtg
      781 gctattttag tttatatctg ttataactgt ttatcattcc tgtagtatat gtaagtaatc
      841 aatatgacat aatattggac aaaaaatctt aaaaaatagg gaaatggata ctattactgt
      901 ttagattaac aacattaaat gaaatgatgt cacaatttga gattatggat accgaaattt
      961 ttgcgaaagt tgaaggaggt ttaggaggtt gggaagaggt agcttttcaa aaaattatat
     1021 ggtttggttg gataatcatg tgcgcattct tatggcggct cttaatactt ctctcacaac
     1081 taatatcaag tttggtctgg ttggaacagt tggaggaaga ttcgctggtt atggtatgta
     1141 tgttcttggt tacccaggtg attatctaga tatcaccaat catagagttg tttc
//
LOCUS       DAVHIU010000205         1175 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVHIU010000205 DAVHIU010000000
VERSION     DAVHIU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.207"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..495)
                     /locus_tag="VVN06_002044"
     CDS             complement(<1..495)
                     /locus_tag="VVN06_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5563512.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTS"
     gene            complement(455..1135)
                     /locus_tag="VVN06_002045"
     CDS             complement(455..1135)
                     /locus_tag="VVN06_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5563513.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANIIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSRK"
BASE COUNT          378 a          241 c          280 g          276 t
ORIGIN      
        1 tgaagtggtt ttaatcatcc gagtgacaag attgatagcc tgcggtagca gtcttcttaa
       61 gacttgtggg atgatgatgt ggtagtaaag ttgaacatta gtcaagccga gtgcctgtcc
      121 actttcaaac tgatgtttag ggagagaagt gatagctcca cgtaccaagt ctcccatttc
      181 agctgttccc cagagggtaa aaacgataat agctgaagtc tcacctgaga tattgatatt
      241 aaagtttcga gccaagccaa agtaaacgat gaagagtagc accagctggg gcatgatacg
      301 gataaattcc agatacaatc gtgttaaaaa tcgtatgatt ctagaatggg aggtcatgat
      361 gattcccatg actgttccga acatcatgga taagaggaca gacaggatgg atatcccaat
      421 cgtaacgccc aatccctgta agattctcag gagattattt ccttgaaaga gtacttggat
      481 tcccgaatcc tgcatggcgg agcctccttt ctatccagct aaagaccagt gagatgggta
      541 gcagcatgat cagataagca actaccaaca tagctagcgc aatgtctgtc tcatagtaga
      601 gaccaatcaa atccttggcg acatacatga ggtcggccaa agccactgct gagaaaacag
      661 aggtttcctt gataaggaaa atgatattgg cactaaagga ggggagtgcc accgctgttg
      721 cttgcggaag aaccacatag cgaaagacct gtagaggtgt cagaccaata gcgaggccaa
      781 tctcctgctg ggtttgactg atggcttcca gcccacttcg gaaagattct gccatatagg
      841 agcctcctaa aaagacaagc ccaagcgttg cacagacttc tgaagatagg acaatcccga
      901 ttcggggaag accgaagtag agaaagaaga gttgaatcaa aaggggcgta ttacgtgaca
      961 attcaatgta ggctgtcgct acttgcgcca aaacagggat tcgataatgt cggatgatac
     1021 taacgattaa accgagcaga aaggatccca aaattcccca aactgcaata tgcaaggtca
     1081 gaaagaatgc cttttgatat agtggtagat attgttcaac aatggaccaa tccaaaaata
     1141 gaacctccca tctagaaata atacagttat tgtag
//
LOCUS       DAVHIU010000206         1155 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVHIU010000206 DAVHIU010000000
VERSION     DAVHIU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.208"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            4..726
                     /locus_tag="VVN06_002046"
                     /pseudo
     CDS             4..726
                     /locus_tag="VVN06_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            876..1109
                     /locus_tag="VVN06_002047"
     CDS             876..1109
                     /locus_tag="VVN06_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU5563514.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGCAAG
                     GVKYGRLLGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          410 a          184 c          239 g          322 t
ORIGIN      
        1 gagatgttag ctgtattaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa
       61 cctaaattaa gcctagaaga ccttcttatg gccactcttc aatatgtgtg agaaccgcac
      121 ttatgaagaa attgcggctg atcttggtat tcacgaaagc aacttaatcc gtcggagcca
      181 atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt tcaaaaactc atcttagtgc
      241 tgaggatacg gtgattgtgg atgcaacaga ggtaaaaata aatcgtccta aaaaaatcaa
      301 ctagccaatt attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca
      361 agtcaaggga gaattgtttc tttggatatc actgtgaact attgccacga tatgaagttg
      421 ttcaaaatga gtcgcagaaa tatcggacaa actggtaaaa ccttggctga cagtaattat
      481 caagggctca tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaaa
      541 ccgctaacag ttaaagataa agcctataac catgcgttat ctaaggagag aagcaaggtt
      601 gagagcatcc ttgccaaagt aaaaacgttt aaaatgtttt caacaaccta tcgaaatcat
      661 cgtaaacgct tcggattacg aatgaatttg attgctggca ttatcaatca tgaactagga
      721 ttctagtttt gcaggaagtc taatgtaatt tttgcaattc aagacgtttc gatgactatt
      781 caagatctgg atgaaaaatt ttaaaaaacg atgatatact aaacttgtca aagttacaac
      841 aagacaaaaa ttaaaaataa aaaagagtat ttgtcatgga tacaaaaatt atggaacaat
      901 ttcatgaaat ggatataact atgttatcta gtattgaagg aggcaagaat aattggcaaa
      961 ctaatgtctt agaaggtggt ggtgctgctt ttggttgtgc agcgggtgga gtgaaatatg
     1021 ggagacttct aggaccatgg ggcgctgcaa taggaggaat tggaggagca gtggtttgtg
     1081 gatatttagc ctataccgct acatcataaa aatgaaagct aaattttatg aaggctatat
     1141 tctttattat tctct
//
LOCUS       DAVHIU010000207         1066 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVHIU010000207 DAVHIU010000000
VERSION     DAVHIU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.209"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..273)
                     /locus_tag="VVN06_002048"
     CDS             complement(<1..273)
                     /locus_tag="VVN06_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEU5563515.1"
                     /translation="MSYNRKIIRTSSYLEIWEYESPIFSNDTTQIDEEHISLKEKKKR
                     RTFEELTTAEQDERIKRISNTRKNSKWKLQRLIDTNFDDRTSFLTLT"
     gene            complement(445..>1066)
                     /locus_tag="VVN06_002049"
     CDS             complement(445..>1066)
                     /locus_tag="VVN06_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEU5563516.1"
                     /translation="AKDLTFLYSWKIDKEQNGIEVINLIGNHDAYYLLGDQAPFSIQN
                     LEVFFAVQGLLQDLKLQVAYQLDDYLISHAGFNQIFDPQKWHFTPYTKEHEEELDILA
                     YTIGPMRGGEALGGSPLWAHFRELELLPNQDFPKQIVGHTPKESIDISKNVIGIDTFS
                     LYIDKDNKYQFIGNGDLLVYDQGHLEIISTNWATDETLKQLPKPQI"
BASE COUNT          321 a          183 c          183 g          379 t
ORIGIN      
        1 tgtaagggta aggaaactag ttctgtcatc aaaatttgta tctattaggc gttgaagttt
       61 ccatttagaa tttttacgag tgttagaaat tctttttatt cgttcatctt gttcagcagt
      121 tgtgagttct tcaaaagttc tacgtttctt cttttctttt agtgaaatgt gttcttcatc
      181 aatttgagta gtatcatttg aaaaaattgg agattcgtat tcccatattt caaggtacga
      241 agaagttcgg ataattttgc gattgtaact cacaaaatca tctccttttc tcgtaatgtt
      301 tgcttatata ataaataaac taggaggagc aaagctcctc cgtacttcta tcatgtgttt
      361 ctaagcatcg ctccgctaaa cacgcttcca gcttgccgac ctccagtcag cagtttcgct
      421 tagtcacatg atgagaagta gtagttaaat ttgtggttta ggtaattgtt ttaatgtttc
      481 gtctgtagcc cagttggttg aaatgatttc aagatggcct tggtcataga ctagcaaatc
      541 accgttacca ataaattgat atttattatc tttgtcaata tataatgaaa aagtatctat
      601 accaataaca tttttagaaa tatcaataga ttctttcgga gtatgtccaa caatttgttt
      661 aggaaagtct tggttgggaa gcagttctaa ttctctaaaa tgtgcccata aaggagaacc
      721 cccaagagct tccccaccac gcattggtcc tattgtataa gctagaatat ctaattcttc
      781 ttcatgctct ttggtgtagg gggtaaaatg ccatttttga ggatcaaata tctgattgaa
      841 accagcgtga ctgattaaat aatcatctag ttgataagcc acttgtaatt ttaaatcctg
      901 caacaatcct tgaactgcga aaaacacttc taaattttga attgagaaag gagcttgatc
      961 tcccaataaa taataagcat cgtgattacc tataagatta attacctcta ttccattttg
     1021 ctctttatct attttccaag agtataaaaa agtgagatct ttggca
//
LOCUS       DAVHIU010000208         1063 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVHIU010000208 DAVHIU010000000
VERSION     DAVHIU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.210"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..627
                     /locus_tag="VVN06_002050"
     CDS             <1..627
                     /locus_tag="VVN06_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5563517.1"
                     /translation="KIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTVTIQNGKEMS
                     STIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPVFKKIE
                     EKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKDVTATVLDK
                     NNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          409 a          138 c          204 g          312 t
ORIGIN      
        1 aagattgttg taaaagattt tgcaagaaat acaaccgtaa aagaattcat tttaaataaa
       61 gatacgggag aggtaagtga attaaaacct catagggtaa ctgtgaccat tcaaaatgga
      121 aaagaaatga gttcaacgat agtgtcggaa gaagatttta ttttacctgt ttataagggt
      181 gaattagaaa aaggatacca atttgatggt tgggaaattt ctggtttcga aggtaaaaaa
      241 gacgctggct atgttattaa tctatcaaaa gataccttta taaaacctgt attcaagaaa
      301 atagaggaga aaaaggagga agaaaataaa cctacttttg atgtatcgaa aaagaaagat
      361 aacccacaag taaaccatag tcaattaaat gaaagtcaca gaaaagagga tttacaaaga
      421 gaagatcatt cacaaaaatc tgattcaact aaggatgtta cagctacagt tcttgataaa
      481 aacaatatca gtagtaaatc aactactaac aatcctaata agttgccaaa aactggaaca
      541 gcaagcggag cccagacact attagctgcc ggaataatgt ttatagtagg aatttttctt
      601 ggattgaaga aaaaaaatca agattaagac aaaagctata gaaaaaaatg gtttatgtac
      661 tgagattaga tagtgaggtg atgacatagt tttgtgaaaa cggccattta taactcaatt
      721 atttagttta cttcacttca ctattgatac tatttggagt tattaatgga cttagtttag
      781 ataactaatg aattgattga aagggttagt attgacaata ttggtcatat tgattagaaa
      841 atagagtcta tcaaaattta aaggctaata gaggtgatga gacaatttca aggaattaag
      901 tagcgtttgg ttgcttaatt cctttttatt tggaaggaag tttttcgtaa ccgcccaata
      961 acgaagtcta ttggacggtt acgatatgat cactgacttc gtcagtctta tctacaacct
     1021 caaaactgtg ttttgagcaa cctgcggcta gcttcctagt tta
//
LOCUS       DAVHIU010000209         1036 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVHIU010000209 DAVHIU010000000
VERSION     DAVHIU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.211"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            79..>1036
                     /gene="pspC"
                     /locus_tag="VVN06_002051"
     CDS             79..>1036
                     /gene="pspC"
                     /locus_tag="VVN06_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEU5563518.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQLEKPKPEVK"
BASE COUNT          411 a          187 c          205 g          233 t
ORIGIN      
        1 aataatgtag caaatttcat atacaattca tattagcagg atatactgta gttaattaaa
       61 attatggaga atatagacat gtttaaatca aatcatgagc gaagaatgcg ttattccatt
      121 cgtaaattta gtgtaggagt agctagcgta gctgttgcca gtctttttat gggaagtgtt
      181 gtacatgcga cagagaaaga gggaagtacc caagcagcca cttcttttaa taggggaaat
      241 ggaagtcagg cagaacaacg tggagaactc gatttagaac gagataaggc aatgaaagcg
      301 gtcagtgaat atgtaggaaa aatggtgaga gatgcctatg taaaatcaga tagaaaacga
      361 cataaaaata ctgtagctct agttaaccag ttgggaaaca ttaagaacag gtatttgaat
      421 gaaatagttc attcaacctc aaaaagccaa ctacaggaac tgatgatgaa gagtcaatca
      481 gaagtagatg aagctgtgtc taaatttgaa aaggactcat tttcttcgtc aagttcagga
      541 tcctccacta aaccagaaac tccgcagccg gaaaatccag agcatcaaaa accaacaact
      601 ccagctccgg ataccaaacc aagccctcaa ccagaaggca agaaaccaag cgtaccagac
      661 attaatcagg aaaaagaaaa agctaagctt gctgtagcaa cctacatgag caagatttta
      721 gatgatatac aaaaacatca tctgcagaaa gaaaaacatc gtcagattgt tgctcttatt
      781 aaggagcttg atgagcttaa aaagcaagct ctttctgaaa ttgataatgt aaataccaaa
      841 gtagaaattg aaaatacagt ccacaagata tttgcagaca tggatgcagt tgtgactaaa
      901 ttcaaaaaag gcttaactca ggacacacca aaagaaccag gtaacaaaaa accatctgct
      961 ccaaaaccag gtatgcaacc aagtcctcaa ccagaggtta aaccgcagct agaaaaacca
     1021 aaaccagagg ttaaac
//
LOCUS       DAVHIU010000210         1015 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVHIU010000210 DAVHIU010000000
VERSION     DAVHIU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.212"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(117..830)
                     /locus_tag="VVN06_002052"
     CDS             complement(117..830)
                     /locus_tag="VVN06_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU5563519.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          320 a          202 c          180 g          313 t
ORIGIN      
        1 gacaagtgtc agatgtaaat tcataattaa cagcttcatt aaaatcaact tcagacattt
       61 caatgtactc ttcaatagta atattagctt tttttgtagt cattagctaa tctcctttaa
      121 aacgtgatct tttcaagaat atttttatct aaacaatcca gcaagtcttg gtaagaatag
      181 acttcgtaag tcggctgggc ttgtgtgtga ttttcgaggt gatgaggatt ataccagata
      241 gtgtcaatcc ccgcattatt gccaccttga atgtcggcgg ttagagaatc tccaatcatc
      301 agcgtctttt ctttactaaa tccagcaatt tgctggccaa tcttttcata aaaaagagca
      361 tccggctttt gagtttgcaa ctgttctgag ataaagactt gattgaaata aggtgctaga
      421 ccagattgag ccaaacgtcc tgtctgaatg gcagtaatgc catttgtcgc agcatacaag
      481 ttataatcac gctcaatgag gctgtccaag agatcatgag cgcccgatag tgtttgtccc
      541 tgctgggcga ggtaaaattg gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca
      601 aaatgagcaa ataaacgaga aaagcgcgtg ttaaccagct cttgtttact gattttcttc
      661 agctccaagt ctttccagag agccttgttc ataggaacgt aataatcttt ataagcctga
      721 atatccgcaa ctccttcttc ttttagaagt tgggtcaaag ccacatcctc agcagcatca
      781 aaatcaagaa gagtgtggtc gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt
      841 tcctttctga aaattcatta agaacattat atcataaagc acctcataca attaactaat
      901 ttaatcactt aaaaaaaatt cgaacacttt ctatacaact gacagctcaa atctttcaga
      961 atagaacaat actaactatc cagcacccca tcttcataaa tatatgtgta attct
//
LOCUS       DAVHIU010000211         1008 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVHIU010000211 DAVHIU010000000
VERSION     DAVHIU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.213"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..143
                     /locus_tag="VVN06_002053"
     CDS             <1..143
                     /locus_tag="VVN06_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563520.1"
                     /translation="LRNDGYLDAEEIKNMDISVIETASRLGYDTSLYRPLSESKKETV
                     LG"
     gene            272..697
                     /locus_tag="VVN06_002054"
     CDS             272..697
                     /locus_tag="VVN06_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563521.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKV"
BASE COUNT          371 a          118 c          221 g          298 t
ORIGIN      
        1 gattgagaaa tgatggatac cttgatgcag aagaaattaa aaatatggat attagtgtta
       61 tagagacagc ttcaagatta ggctatgata caagtttata tcgtcctttg tcagaaagta
      121 aaaaggaaac ggtattagga taatatatta attcgactga acaactttta gaaaataaca
      181 gaatttcgca agggaagtat gaggaactgt tactagatgc tttcagatat gatattgtat
      241 atgggctata tgaagaggag ggagttgtcg tttgactagt cgtgtattta ttgatgcaga
      301 ttgtatttca gtatttttat gggttggcac tgaacatctt ttagaaaagc tctattcggg
      361 taaaattgtt attccacaag aggtgtatga tgaaatcaat atacctacaa ttccccattt
      421 aaaatctagg atagaccagt tggtagctaa gggttcagct gagattgtga gcatagacat
      481 tggaactgaa gaatacgcat tatatagaga tttaacaaga aatcatgata gtaacaagat
      541 tattggtaag ggagaagcgg cttctatttc cttagcgaaa aagcataatg ggatattagg
      601 aagtaataac ctaagagatg ttaaatcata tgtagaagaa ttttctttag aacatatgac
      661 aacaggagat atactgattg aagcgtttaa agtgtaattt attactgaac aagagggcaa
      721 tcatatctgg aataatatgc ttaaaaagag aaggaaaatt ggtgcaaatt cattttcaga
      781 ctatcttcgt ggaagtattc atcaaaatag acaaaaataa atttggataa atcgaactca
      841 ctattcagga ggcatatgag caattcgaaa aagaaaagtg tcaaattgag cctataggag
      901 tagaagtgaa atagtaagtc ctgcatagtg gatgagagaa aagttctcct tgaagttttc
      961 ctgaactatc agtcgcatgt caaacgatat gtagggtaat gtgagagg
//
LOCUS       DAVHIU010000212          996 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVHIU010000212 DAVHIU010000000
VERSION     DAVHIU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.214"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            40..903
                     /locus_tag="VVN06_002055"
     CDS             40..903
                     /locus_tag="VVN06_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5563522.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
BASE COUNT          348 a          202 c          179 g          267 t
ORIGIN      
        1 ttattatttg ttaacttttt tcacagaaag gagaaaatca tgcgttatga tttcggaaag
       61 gtctataaag aaatacgtga gtcaaagggg ctgacccaag aagaggtctg tgggggtgtt
      121 ctctcaagaa ccagcttatc aaaaatcgaa agtggcaaga caaccccaaa atatgaaaat
      181 atggaatttc ttctccgtca aatcaatatg agttttgaag agtttgaata tatctgtcaa
      241 ctttatcaac caagtcaacg cacagagatt atgcaaacct atctcaatat gcgttctatt
      301 atcgggacca gcgatcttgt caatttattt caaaaatgcc aagactacct caagacccac
      361 cacgatctcc ctatagaaga aatcagagat atgcttgaag ttgtcattta tatccgtcaa
      421 cacggggcag gggaactatc agatcacgca gaacaggttg tcaaaaaact ttggagaaaa
      481 attgaaaaac aagatacatg gtatgagagt gatctaaaaa tcctcaatac cattcttttc
      541 agttttccca ttgaatacct ccatctcatc actggaaaaa tattgcaacg cctagaagtc
      601 tataaaaact atcaacattt atatgacttg cgaatagcaa tcctgctcaa cctttccaca
      661 ctctatctat acaatcaaga taaaaacatg tgtaagcaaa tctgctacac tttactggag
      721 gacgccaaga ataagaaaag ctacgatagg ctggctatct gctatgtccg cattgggatt
      781 tgtacagacg attctaaact tatccaaaaa gggttctccc ttctggagct gaccgaggaa
      841 acttctatgc tgtctcatct caaaaaagaa gtagagatct attaccaagc gaaggaaaga
      901 taaaaaagcc gagggagctc ctcgaccttt tcatagaatc gccgaacgat ttaacgagaa
      961 agtatgactt ttacgtttat cccaactcaa ttatga
//
LOCUS       DAVHIU010000213          975 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVHIU010000213 DAVHIU010000000
VERSION     DAVHIU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.215"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            485..710
                     /locus_tag="VVN06_002056"
                     /pseudo
     CDS             485..710
                     /locus_tag="VVN06_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          364 a          201 c          175 g          235 t
ORIGIN      
        1 ctacagcgta ctattccaaa ctcaatcaac tataaaacaa tatagactag taatggttgg
       61 actcaataag aattgggtac taagataggt ataagtaaaa acaccattag aaattatgaa
      121 aaaagggtta ggtctactaa gaaaaatact atatttgact tagttaaagt attcagtagc
      181 ttgattgatg ccctctttcc tccagttcaa aaggattctc ctagcgatat ccaatctatc
      241 tacgaccaac gggcaccacc cagacaaggt aaagtcctaa cctatgccga gaggcaactg
      301 tataagcaga aaaacgaagg atcgtaaaaa tcatcagact ggacgactac agacagacta
      361 cttactctcg tgttaggtag tttatctctg caggtagccg ctcgatgcag gacgacgatt
      421 tagttatgga gtttccatta tcccagacga ctacgaccca atcgcttatg tcgtcagcaa
      481 gtccatggag gcaaagataa gaaacagcga ctacctattt atcaagaata ccccacagat
      541 tgactataat accatcggca tcttccaagt agacggcgct aactatgtca agaaactccg
      601 ccaaggctac ctcaaaaaaa ctcaatcccg aataccccga catccaccta gacgaaagca
      661 accatattcg taccatcggg aaagttgtca ggctatatag ggagaattaa aaaagggggg
      721 agaatggcga tataaaacaa acagatttta aaagattcga aaaactttga aaaagttcat
      781 tctacatttc aaaaaaagaa taagtgacct aaaatagatt agattttgtt aaacaatagt
      841 gatatgtaaa tttagagaaa ggcatttaag atcactttgc aaaaggcaaa tactaaacta
      901 gagaaaccga taaaccagcc ccttagttcc catattttca ggcacacact ccttagtaca
      961 ctagctgaga agaat
//
LOCUS       DAVHIU010000214          945 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVHIU010000214 DAVHIU010000000
VERSION     DAVHIU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.216"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..918)
                     /locus_tag="VVN06_002057"
     CDS             complement(<1..918)
                     /locus_tag="VVN06_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5563523.1"
                     /translation="MFLSLKLGTKEINIRDFLAAFGMGNTNDDFIKSIIYKRIPRTIF
                     AILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIALSFLGISSSISHISFA
                     IIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRF
                     WQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSL
                     AIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDI
                     IGRMLGKPGEIEVGIITAIIGGPVLIYVTM"
BASE COUNT          342 a          180 c          141 g          282 t
ORIGIN      
        1 catagttaca taaataagta ctggaccccc gattattgca gtaattatcc ctacttcaat
       61 ttcacctggt ttacctaaca tacggccgat tatatcacat ataagcaaga gctctgcacc
      121 tataaaagat gaagaaatgg tcattgtgcg tatatctttg cttataaata agccacaaaa
      181 gtgaggaact ataagaccta cgaagccaat aggtccacca attgcagtaa tacttgaaca
      241 taaaagcaca cttgcaatta ttgcaagtga tcttatccta ttaacattaa ctccaagacc
      301 aacagccatt tcatcaccca tagctaaagc gtttaaatct gatgaaataa atatagctat
      361 caagtgacct aaaattataa aaggtagtag tgtagatata gaagataatg tagctgctcc
      421 aaggctacct atttgccaaa atctaaattt gtctaagacg ttattattcg gtaaaattaa
      481 aaaacttaca aaactgctta aagccatact aacacaagtt cctgataagg ctagttttat
      541 aggggtaagg cctgcttttc cgcttacagc aatcgcgtat acaaaaattg cacttactaa
      601 gccaccaatg attgcaaaac ttatatggct tatgcttgat gaaattccta aaaaagaaag
      661 agcaattact acactaagac ttgctcctgt gtttataccg agtatacctg gatcagctat
      721 tgggtttcta gtaactgatt gcatcaacac accgcttatg gcaagactag aacctgctaa
      781 aattgcaaaa atagttctag gtattctttt atatataatt gatttaataa aatcatcatt
      841 tgtattaccc attccaaaag ctgctaaaaa atctctgata ttaatttctt ttgttccaag
      901 ctttaatgac aaaaatattg taataagaag acctaggcag attac
//
LOCUS       DAVHIU010000215          932 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVHIU010000215 DAVHIU010000000
VERSION     DAVHIU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.217"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            29..634
                     /locus_tag="VVN06_002058"
     CDS             29..634
                     /locus_tag="VVN06_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5563524.1"
                     /translation="MKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTP
                     ARTPNSVPFGRIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQ
                     KNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGASGSTVYDASN
                     RVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
BASE COUNT          310 a          118 c          188 g          316 t
ORIGIN      
        1 agcttatgta gagtcagaat ttcgtggtgt gaaaaatggt aaagatgtaa cttatcgagg
       61 gagtgccaat tttataaagg ataatgtcct tattactgct gctcataata tttataaaca
      121 tgagtttggt aaagatgcag aagaactata tgttactcct gctcgtactc caaatagtgt
      181 gccgtttggt cggataaaag taaaagaagt tcgttatttg aaggaattta gaaatttaaa
      241 ttctaaggat gcaagggaat atgacttggc tttattaatt ctagaagagc ccattggtgc
      301 aaaattaggg actttgggtc ttcctactag tcaaaaaaat ttgacaggaa taactgtgac
      361 tatcacaggc tatccatcat ataattttaa aattcatcaa atgtatacag ataaaaaaca
      421 agttttaagt gatgatggca tgttcttgga ctaccaagtt gatactttag agggggctag
      481 tggatctaca gtttatgatg ctagtaaccg cgtagtagga gtgcatactt taggagatgg
      541 agctaatcaa attaacagtg cagttaaatt aaatgaacga aatttgccat ttatttattc
      601 ggttcttaaa ggttactctc ttgaaggatg gtgaatgggt taggtaatga aggctaattg
      661 taaactgtga tggatactta actttgtata ataggtggat aaaagtcttt aaaataggaa
      721 aaaacgcata ttattaggtg tttaaaaacc ttgataatat gcgttttata atggaaagat
      781 ttcttcaatt tcgtcttgta tagtagattg aaactataat agtacacctc tacttctaaa
      841 acattgttag aaattgattt gactgtcctg atcgatttgt cctgttctta tttcgtttta
      901 ctataatgta atcgtccaat aacgaagtgt tt
//
LOCUS       DAVHIU010000216          917 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVHIU010000216 DAVHIU010000000
VERSION     DAVHIU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.218"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>917
                     /locus_tag="VVN06_002059"
     CDS             <1..>917
                     /locus_tag="VVN06_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5563525.1"
                     /translation="VFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVA
                     TIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLN
                     FEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGM
                     EKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRAN
                     YLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLL
                     GKINAIKNGAVAVIPDNTPLAASCTPTPL"
BASE COUNT          355 a          131 c          162 g          269 t
ORIGIN      
        1 agtttttagc gcttgttctt ctaattctgt taaaaatgaa gaaaatactt ctaaagagca
       61 tgcgcctgat aaaatagttt tagatcatgc tttcggtcaa actatattag ataaaaaacc
      121 tgaaagagtt gcaactattg cttggggaaa tcatgatgta gcattagctt taggaatagt
      181 tcctgttgga ttttcaaaag caaattacgg tgtaagtgct gataaaggag ttttaccatg
      241 gacagaagaa aaaatcaaag aactaaatgg taaagctaac ctatttgacg atttggatgg
      301 acttaacttt gaagcaatat caaattctaa accagatgtt atcttagcag gttattctgg
      361 tataactaaa gaagattatg acactctatc aaaaattgct cctgtagcag catacaaatc
      421 taaaccttgg caaactttat ggagagatat gattaaaatt gattcaaaag ccttaggtat
      481 ggaaaaagaa ggtgatgagt taatcaaaaa tactgaagct cgtatatcca aagaattaga
      541 aaaacatcca gaaatcaaag gaaaaatcaa aggaaaaaaa gtattattta ctatgattaa
      601 tgctgcagat acatcaaaat tctggattta tactagcaaa gatccaagag caaattattt
      661 aacagattta ggtctagttt tccctgaatc attaaaagaa tttgagagtg aagatagttt
      721 tgcaaaggaa atttctgcag aagaagcaaa taagataaat gatgctgatg taatcataac
      781 ttatggtgat gataaaactc ttgaagcttt acaaaaagat cctcttttag gtaaaataaa
      841 tgcaattaaa aatggtgccg ttgctgtaat tccagataat acaccgttag cagcctcatg
      901 cactccaaca ccacttt
//
LOCUS       DAVHIU010000217          887 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVHIU010000217 DAVHIU010000000
VERSION     DAVHIU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.219"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..530)
                     /locus_tag="VVN06_002060"
     CDS             complement(<1..530)
                     /locus_tag="VVN06_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5563526.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNRLLIYLNTSGIKIL"
     gene            complement(532..>887)
                     /locus_tag="VVN06_002061"
     CDS             complement(532..>887)
                     /locus_tag="VVN06_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5563527.1"
                     /translation="MIRAVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVV
                     VLNFNSLLSDYDLSVFLYHPLFQPLGIVIKQSTDETATLNAQAMMFVYSVILMIMSSA
                     ALYLSSLFQGKRGKR"
BASE COUNT          320 a          180 c          132 g          255 t
ORIGIN      
        1 tctaaaatct taatacccga tgtatttaaa tatataagta aacgattata accttctaaa
       61 gaactaaaaa ccaagtagta atcagcaatt ttagttttcg agtttaaata atctttaaga
      121 ggagtaaata gagttaaaac tgctatgagt acaactccgg ctaaaatagc accttcataa
      181 aaaccaatgc ctatagctaa accaatagtt gctgaagacc ataatccagc tgctgttgtt
      241 aaacctttaa ctttttgatt agttaccatg attgttcctg cccctaggaa acccaagcca
      301 ctaattactt gggctcccat tcgactagga tcaccgctac cataacgact agtgatgaac
      361 tgatttgtca tcattacaac acaagttccc aaacaaacta gtaagtaggt tctaatccct
      421 gctgcttggt ttttgactcc tcgctcatag ccaacaatgc caccgaaaag aatcgctaaa
      481 aagcacctaa gaagtatttc ccaaatactc agttcgtatg aaagattcat attatctctt
      541 acctcgttta ccttggaata ggcttgataa ataaagagct gcactagaca taatcattaa
      601 aattacagaa taaacaaaca tcattgcctg tgcatttaaa gttgctgttt catcagtaga
      661 ctgtttaata acgattccca atggttggaa aagtggatgg tacaagaata cagataagtc
      721 atagtcagat aataaagaat taaagtttaa cacaactact gataatacga ctggtaaaat
      781 atacggtata ataactcgca ccatagtgta gaaactagat gcacccatac tacgtgcagc
      841 ttcttccata tcattatcta tactgaaaaa tacagcccga atcattc
//
LOCUS       DAVHIU010000218          883 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVHIU010000218 DAVHIU010000000
VERSION     DAVHIU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.220"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..597
                     /locus_tag="VVN06_002062"
     CDS             <1..597
                     /locus_tag="VVN06_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="otitis media-associated H10"
                     /protein_id="HEU5563528.1"
                     /translation="KNSDLGSLSHYLSDKYVATTPNSIARIVRLVVEQMQARYQTMRD
                     NFHYGSNFADHGFKPVWLIFDEMGAFQASATDKKSKEVITEVMDGIKQIILLGRQAGV
                     FILISAQQMRAETLNTDLRDNLGLRIALGANSIEGYRMVFGTATPDKFKSIEEKGAGY
                     LYMQGSGKEKAQYWESPYLDTTQFDFIKELQLYVTKSK"
BASE COUNT          329 a          140 c          159 g          255 t
ORIGIN      
        1 aagaacagtg atttaggtag tttatctcat tatttaagcg ataaatatgt agctacaacc
       61 cccaatagta ttgctcgtat tgtacgttta gtagttgaac aaatgcaggc acgatatcaa
      121 accatgcgtg ataactttca ttacggatca aattttgccg accatggctt taaaccagta
      181 tggcttatat ttgatgaaat gggagcattt caagcttcag caacagataa aaaatctaaa
      241 gaagttatca ccgaagtaat ggacggtatc aaacaaatta tcctactagg gcgacaagca
      301 ggagtattta ttcttatatc tgctcaacaa atgagagctg aaactctaaa cactgatctt
      361 agagataatt taggactgag aatagcttta ggagcaaact caatagaggg ataccgcatg
      421 gtatttggca cggccacacc tgataagttt aaatctattg aagaaaaagg agcaggatat
      481 ctttatatgc agggctcagg gaaagaaaaa gctcaatatt gggaaagtcc ttatcttgat
      541 acaacgcaat tcgattttat aaaagaacta caattatacg taacaaagtc aaaataattt
      601 caatagatat ttttgcttaa attcaataga ttttcagcaa aaatatctat tcctcatcgc
      661 ttaagcgatg acaaaaagaa acgaggtatt atacgaaaat taacgatttt cttaaaccag
      721 aattattggg aaataaattc gtggcagtaa aaggatatac agaagttttg gacagagaaa
      781 caaataaacc tgtagcacta cgtcttaatg tttcaattca agatgaggat tctgatttct
      841 ttatggaaat gatccagata aaagttaata cgttatctcc tac
//
LOCUS       DAVHIU010000219          871 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVHIU010000219 DAVHIU010000000
VERSION     DAVHIU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.221"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..648
                     /locus_tag="VVN06_002063"
     CDS             <1..648
                     /locus_tag="VVN06_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU5563529.1"
                     /translation="MPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYT
                     LEDLLATVKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPDEDKGHDEVSEPTHP
                     ESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEA
                     LLEKVTDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT          322 a          138 c          171 g          240 t
ORIGIN      
        1 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
       61 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      121 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      181 gaacgtccgc attcagataa tggttttggt aacgctagcg accatgttcg taaaaataag
      241 gcagaccaag acagtaaacc tgatgaagat aagggacatg atgaagtaag tgagccaact
      301 caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc agataatctt
      361 tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac tacagatgag
      421 gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga tgcggaggcc
      481 ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac attgactggt
      541 ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc agaagtagat
      601 agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta aaatgaatgg
      661 agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa aaacgtaaca
      721 gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta aatggttaaa
      781 taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac ttcatcattt
      841 attgaaatga gggatttatg aaatttagta a
//
LOCUS       DAVHIU010000220          865 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVHIU010000220 DAVHIU010000000
VERSION     DAVHIU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.222"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            316..501
                     /locus_tag="VVN06_002064"
                     /pseudo
     CDS             316..501
                     /locus_tag="VVN06_002064"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          333 a          164 c          122 g          246 t
ORIGIN      
        1 gccacatagt actgaactct ctccaaaaac ggttatgcct ctttgaatag ggcgttatca
       61 caagaaaagc atctccacgt ttcaacttca tatggctcaa aaacaatcaa ttgatgctaa
      121 aacctgtacc tagatgtttc ggttcataaa accatgaaac tgtaaaagtg gatgaaattg
      181 atagcgatag tcaaatcaag aggcatcata actctaaaaa gtcacaatat ataagttcat
      241 cctcggaaaa atatcattct aattgttgaa atgcctacat gaaaagaaac gtcaaatact
      301 catgaaacaa cgaatacagg tatcaaaact atgacaaaac aagtccctaa atttactaaa
      361 gacactgctc aactttacac ctgtaaatgg ttgttgtata ataaagttac aaagatgtac
      421 gaccacactg ttgtaaatca tagtgttcgc gaatatatta ctgatagcat ttctacaaat
      481 acaattgaaa aggctggata ggctttaaac gaaatatgtc agtgttttgg cattcctagc
      541 cttcatatca tttaaagaat tgtatagaca aaatttttcc caatacagac actcgtaaca
      601 actgcttcat ttttctacca acatatttag gaacaggata agatacaaga ggattaatcc
      661 atagatcagt tctataccaa tctaagacaa ataagctaaa aaaacgattg ataataagca
      721 aatagattcc aaattttctc tatctgctca ttttaataaa caatactagt gtaactatcc
      781 ttccagtcag aagcttgtca aatcacaccg aaaattcttc taaaatttat ctcgttaggc
      841 aatcaagcaa aaactcgacg atagt
//
LOCUS       DAVHIU010000221          863 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVHIU010000221 DAVHIU010000000
VERSION     DAVHIU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.223"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..805)
                     /locus_tag="VVN06_002065"
     CDS             complement(<1..805)
                     /locus_tag="VVN06_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563530.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIES"
BASE COUNT          308 a          154 c          120 g          281 t
ORIGIN      
        1 ttgactcaat tggattaatt gaatacaatt ttaaaaaaat aggcttcgta ccaataatag
       61 aggataattg acctcctaat tcacctagcg ctgtaaaaat aacacctgtt agtgctccta
      121 ttgcttcaat agtaccaatt ttcactattc cttttattgc aagatagcct gttaaaaaaa
      181 cgagagatat ctgaaaaaaa atattgagaa agaagctaat agcgcctgct aacgtttcta
      241 cagttgtctt tctttgtata accatcttta ataaaattcc tgcttcttta attttcttag
      301 gcaatacata taaaagattc aaggacgcta acacatcaaa tccattcaat atagtctcac
      361 tagattttaa aaaagcttca ttttggctag ttaaatttag actaacttct cgcattttcg
      421 atgcaaagat ttttggtaca agtagcataa tcattaatga aaacaaggtg gctacagtca
      481 atgaccaatg atagtgatta agagtcacaa ctgcaaatat agtaccagaa attcctttta
      541 ttactaaaaa aagttgttta aaagcctgat catttaaagt ctgaacatca ttatttagcc
      601 acgaaagata tgttcctgat gatttactat gaaattcttg ataggtagag ttagagatgt
      661 ctgtggcaac tctatttcga atctctagat taaactcttg gatcacttca acctgataat
      721 ttttcactac ccagtcaagg aatattatcc cacaccagac aatcatttgg tagattgaca
      781 atttcaaaaa ccgctctaaa ttcatcgcaa ttaattcatt caacaccaga gcattaatag
      841 ttgctgcata aattagcaat aat
//
LOCUS       DAVHIU010000222          863 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVHIU010000222 DAVHIU010000000
VERSION     DAVHIU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.224"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(1..863)
                     /locus_tag="VVN06_002066"
                     /pseudo
     CDS             complement(1..863)
                     /locus_tag="VVN06_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          266 a          150 c          185 g          262 t
ORIGIN      
        1 tttatctttg aggaaggttt taaagacagt ctgaaaaata ggatgaacct gcttaagatt
       61 gtcctcgata agttcgaaaa atttctctgg ttccttattc tggaagtgaa aaagcaagag
      121 ctgatagagc tgatagtggt gtttcaagtc ttccgaataa ctcaaaagct tgtttaaaat
      181 ctctttattg gttaagtgca tacgaaaagt aggacgataa aaacgtttat cactcagttt
      241 acggctatcc tgttgtatga gcttccagta gtgcttgata gccttgtatt catgggattt
      301 tcgatgaaac tgattcatga tttggacacg cacacgactc atagcacggc taagatgttg
      361 tacaatgtga aagcgatcaa gaacgatttt agcattcggg agtgaaacag tctaggagac
      421 tgtttcagcc tgagcctaga aatttgaaag cgaagctgtt tagccaagtc atagtaaggg
      481 ctaaacatat ccatagtaat gattttgacg cgacatcgga cggctctatc atatttaaga
      541 aagtgatctc ggatgacagc ttgtgttcta ccttcaagaa cagtgatgat atcgagcttt
      601 tcaaaatctt gcgcaataaa gctcatctcc atctcccgat tgaaacagtc actccccgga
      661 ctgtttcaac gtcccaagac ataatcttag gcagatttct aaaattacac tcaaagtgga
      721 agtcattgag ctttcaaatg acagttgaag ttgagatgga aagctgatgg gcaatatcag
      781 tcatagaaat cttttcaatt aacttttgag caatcttttg gttgatgata cgagggattt
      841 ggtgattctt cttgacgata gaa
//
LOCUS       DAVHIU010000223          858 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVHIU010000223 DAVHIU010000000
VERSION     DAVHIU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.225"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..186
                     /locus_tag="VVN06_002067"
     CDS             <1..186
                     /locus_tag="VVN06_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU5563531.1"
                     /translation="EDAKNKKSYDRLVICYVRIGICTDDSKLIQKGFSLLELTEETSM
                     LSHLKKEVEIYYQAKER"
     gene            complement(238..825)
                     /locus_tag="VVN06_002068"
     CDS             complement(238..825)
                     /locus_tag="VVN06_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5563532.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT          233 a          174 c          175 g          276 t
ORIGIN      
        1 gaggacgcca agaataagaa aagctacgat aggctggtta tctgctatgt ccgtattggg
       61 atttgtacag acgattctaa acttatccaa aaagggttct cccttctgga gctaaccgag
      121 gaaacttcta tgctgtctca tctcaaaaaa gaagtagaga tctattacca agcgaaggaa
      181 agataaaaaa gccgagggat ttcctcgact ctttacatat tatttgttcg ttagttctta
      241 ataccagcca ttgttaagcc agaagttttt agcagcagtc catgaaccgt aacgtcctgc
      301 aacgtaggca tctgctacac gttcttggtt ttcagctgag tagtcaccgt tcaggtatga
      361 atctgttaat tggtaacgtc cgatataacg tccatttgta gctgtatagc taccacctga
      421 ttctttttga gcgatccatt ctttggcttc tgcttcagat ccgcttacag ttgaaacaac
      481 tgtttcactt actactggag tttctgctac tggagctgaa acagtttcat cttgagcggc
      541 tggtgccgca taagtagctg gcgctggtgt tgcaacaggc gctacagggc catcgataac
      601 caactcttga ccaacataaa tcaaatgaat gttatcaatg tggttgtttt ctgccaattt
      661 ttcaactgtt gtgttgtgag tttcagcgat ttctgaaagt gtatcacctt ctttaacagt
      721 gtaagttgat gattcttgag cagatacaaa tgatggagca aatactgcaa acaaggcagc
      781 tactcctgca agagttgctt taatcttttt agttattgat ttcatattcg aaaattctcc
      841 ttctttctat agttctat
//
LOCUS       DAVHIU010000224          853 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVHIU010000224 DAVHIU010000000
VERSION     DAVHIU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.226"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            242..580
                     /locus_tag="VVN06_002069"
     CDS             242..580
                     /locus_tag="VVN06_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU5563533.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
BASE COUNT          277 a          113 c          195 g          268 t
ORIGIN      
        1 attgattagt ctggccataa tctttccatg gggctggtcg atataatgta agggagcttg
       61 agttgttaaa ggaggcttag tccttggaca aaaatctgct gtactacaat gcttggaaag
      121 atatcaagaa caaagaatga tacgattgaa actaaactga ttcaagaact actgggcagt
      181 gattgaggtt tgggtgacat caaagaaaag ttagattaat tctaaagaat tgagtatttc
      241 tatgttaaaa aaatatcctt gtacgatgca acatgatcag tcagattgtg ctgcggcagt
      301 tgtttcaaca gttcttttat cttacaaaaa ggaactatca atcatgaaga ttcgggaaat
      361 tattggtaca gacatgtatg gaacgactgt cagtggtatt gtttcaggtc tgaataagtt
      421 gaattttaca gtaaaagctg ttcgagtagc actggaagat ttgactccaa aattaacatt
      481 tcctgcgatt cttcaagtta agaatgattt aggtcaaaat cattttgtgg tattacatag
      541 tataaaggag aaaataaagg gaactcgtat taccaaataa ttatgttgct cttgagcaag
      601 aggagatgat gtatcttgat gggggggggc ttatttaagc aagagtgctt gtcaaggaat
      661 ttgcgcagct ttagctatga gtccaggaac ttttatagca ttgactggag ctgcagtttt
      721 aacaaaaaaa ctaataaact atattaaggt tggaggcctt ggaggttggc ttattggtgc
      781 agcagcaggt gtattggctc ttatcttttt gataaaaatt tgtgtagata ctacgataaa
      841 ggttctatta aac
//
LOCUS       DAVHIU010000225          786 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVHIU010000225 DAVHIU010000000
VERSION     DAVHIU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.227"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>786
                     /locus_tag="VVN06_002070"
     CDS             <1..>786
                     /locus_tag="VVN06_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEU5563534.1"
                     /translation="SFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEK
                     EIALKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIY
                     NEVLVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKL
                     PIELLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGD
                     LFALGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQI"
BASE COUNT          267 a          109 c          161 g          249 t
ORIGIN      
        1 tctttcgttt cagatatggt aagtacgatt aaacatttga atagttttgg aagtattttg
       61 tcatctattg tctttatctc tctccttttg accttctttg caatgaattc tcataaagtt
      121 caaatggaga aagagattgc tttgaagcag aagaaatttg aacagaaaca tttacagaat
      181 tacacagatg aaattgttgg tctgtataat gaaatccgtg gttttcgaca tgattatgct
      241 ggaatgcttg tcagcatgca gatggcaatt gacagtggta atttacagga aattgacaga
      301 atttacaatg aagttttagt caaagcaaat cataaattgc gttcagataa gtacacttac
      361 tttgatttga acaacataga agactcagct ttacgaagtt tggttgctca gtcaattgtc
      421 tatgctcgaa ataatggtgt agagtttaca ctggaagtaa aagatacgat taccaagctt
      481 ccaattgaac tattggattt ggttcgtatc atgagcgttt tattgaataa tgctgtcgaa
      541 ggatcggctg atagctataa aaagcagatg gaagtagcag ttattaagat ggaaactgaa
      601 acagttattg tgattcagaa ttcatgtaaa atgacgatga ctccttcagg agatctattt
      661 gccttaggat tctccactaa gggaagaaat cgcggagtcg gattaaataa tgtgaaagaa
      721 ctactagata agtacaacaa tattatttta gaaacagaga tggaaggcag tacatttaga
      781 caaatc
//
LOCUS       DAVHIU010000226          780 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVHIU010000226 DAVHIU010000000
VERSION     DAVHIU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.228"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            146..>780
                     /locus_tag="VVN06_002071"
     CDS             146..>780
                     /locus_tag="VVN06_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU5563535.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLATNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLER"
BASE COUNT          218 a          171 c          142 g          249 t
ORIGIN      
        1 tctaaaaacc atccagaatc cttgccttag cttagatcct ggatggtttc ttttttcacc
       61 caatgggtgt tttttactag acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa
      121 aaacacaaaa agaaaggaaa ctcacatgaa cagtttacca aatcatcact tccaaaacaa
      181 gtctttttac caactatctt tcgatggagg tcatttaacc cagtatggtg gtcttatctt
      241 ttttcaggaa cttttttccc agttgaaact aaaagagcgg atttctaagt atttagcaac
      301 gaatgaccaa cgccgctact gtcgttattc ggattcagat atccttgtcc agttcctctt
      361 tcaactgtta acaggttatg gaacggacta tgcttgtaaa gaattgtcag ctgatgccta
      421 ctttccaaaa ttgttggaag gagggcagct tgcttcacag ccaaccttat cccgttttct
      481 ttccagaact gacgaggaaa cagtccatag tttgcgatgc ctcaaccttg aattggtcga
      541 attcttttta cagtttcacc agctaaacca actcattgta gatatcgatt ctacccattt
      601 cacaacttat ggcaagcaag aaggtgttgc ttataacgcc cactatcgtg ctcatggcta
      661 tcatcctctt tatgctttcg aggggaagac aggttattgt ttcaatgccc agcttcgtcc
      721 tggtaatcgt tattgttctg aagaggcaga cagctttatc acacctgttt tagaacggtt
//
LOCUS       DAVHIU010000227          759 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVHIU010000227 DAVHIU010000000
VERSION     DAVHIU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.229"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..605)
                     /locus_tag="VVN06_002072"
     CDS             complement(<1..605)
                     /locus_tag="VVN06_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU5563536.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGV"
BASE COUNT          232 a          165 c          104 g          258 t
ORIGIN      
        1 tttaccccca attctctctc aagtgtaatc agtaatccca aacaaaagtg agcaagttct
       61 ccgtcaagag gctgaacctt attctgtgta atttgctcta aatccaactg attaaacatt
      121 ggcagtagat ttagaatggc tgaacgaatt tgagcctcat ccccatcaat aaaaagcttt
      181 cgttgtctca tttttaaatc taacccataa attgctagac tctcttgtaa tatctgacga
      241 tcttttagta ctactgactc gcttacgtag aattcctctc ctaaatcata aatagaatgt
      301 ggcttaggag cagtcaacaa gagacgttct agtaattttt cacgccttac ttcaggatca
      361 aaagcctctg taaaattccc atcaacgtcc acgagggaac ttcttggatt taatttgaag
      421 cctctgcctt tttcagataa aatgaatgct tctaccggac aatctttgtt gattaccttg
      481 acaaggcgat aaaccgtttt atcagagcaa tttaattttt ctcccaatac tttagcagat
      541 atataatcct tactatctcg caaaaggagc aacaacgttt ccatttgttc ttgttttcta
      601 gacacgatac acctccttta tgattccatt atatcgcttt caaaaaataa tatacttcgc
      661 attttgtcct ttgcaatgga catttttgtt tttatcaaaa atatgatatc aaatagaaca
      721 gaacctcact atgccaaaat cattttacct tttcttcat
//
LOCUS       DAVHIU010000228          742 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVHIU010000228 DAVHIU010000000
VERSION     DAVHIU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.230"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>742
                     /locus_tag="VVN06_002073"
     CDS             <1..>742
                     /locus_tag="VVN06_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5563537.1"
                     /translation="DIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGD
                     TEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAI
                     SNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQRI
                     IEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVL
                     LEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT          282 a          101 c          132 g          227 t
ORIGIN      
        1 gatattcctg atacttggga gtgggtgagg ttttctacat tggttgaaat tgtcagaggt
       61 ggctctccac gaccaatcaa agattatctt acttctgaag tagatggaat aaattggata
      121 aaaataggtg atactgaaaa gggtgaaaag tatataaata atgttaaaga aaaaatcaaa
      181 aaatcagggc ttaacaaaac tagatttgta aaaaaaggta catttttgtt aactaattct
      241 atgagttttg gtagacctta tattttgaat gttgatggtg caatacacga tggatggttg
      301 gctatttcga actatgaaaa ctcattaaat aaagattacc tattctatat tctttcatca
      361 aatgtagttt attctcaatt tctatctcta attagtggag ctgttgtgaa aaacttgaat
      421 agtgataaag ttgcttctat tcttatccct ctccctccgc tagctgaaca acaacgcata
      481 atagaagcaa tcgaatcagc tttagaaaaa gtagatgaat atgctgaaag ttataataga
      541 ctagaacagc tagataaaga atttccagat aaactaaaaa aatctattct tcaatatgct
      601 atgcaaggaa aattagttga acaagaccca aatgatgaat cagtcgaagt tttacttgaa
      661 aaaatacgag cagaaaaaca aaaactcttt gaagaaggca agattaaaaa gaaagatttg
      721 gacatttcta ttgtttccca ag
//
LOCUS       DAVHIU010000229          735 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVHIU010000229 DAVHIU010000000
VERSION     DAVHIU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.231"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..445
                     /locus_tag="VVN06_002074"
     CDS             <1..445
                     /locus_tag="VVN06_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563538.1"
                     /translation="LTNQFPLVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPI
                     QMELVSDEMIQEIEQATKNSNIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQD
                     LSEYIQSSSYHFEEAKYQDIPNFSELSETEDFEIIPRIEKQEGQK"
     gene            460..>735
                     /locus_tag="VVN06_002075"
     CDS             460..>735
                     /locus_tag="VVN06_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5563539.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQT"
BASE COUNT          274 a          110 c          127 g          224 t
ORIGIN      
        1 acttacgaac caatttcctt tggtacaaaa aaataagagt ggctacttat ttgaatacgc
       61 tcagatgaat acactttatc ttaatcaaca ttcttcctac aaaaatgatg aagatagtca
      121 ttcctttcca attcagatgg agttagtttc tgatgaaatg attcaagaaa ttgaacaagc
      181 tactaaaaat tcgaatatcg aaaaatttat gattttaact tatgaggcgg actcagaaaa
      241 caatattata tctgtagatg ttgttatgcc tgatgcacga actggtcagt tacacttgat
      301 tcaagatttg tctgagtata ttcaatcaag ttcgtaccat ttcgaagaag ctaaatatca
      361 agatattcct aatttttcag aattatcgga aacagaagat tttgaaatta ttccaagaat
      421 agaaaaacaa gaaggtcaaa agtagtaagg agtatgtaaa tgtttaatgg tcgggtattg
      481 aaagaattac ggctgttaaa tggtttaagt agagcagaat tagctcagag aattaattta
      541 acggaacaag ccatttggca gtttgagtcc aacgaaacga aacctaaatt atcaaccaaa
      601 atgcatttgg ccaaccaatt tcatgttgat ttaacttatt ttgaacagga agaagagagc
      661 attcgatttg attcttctgt aattgccttt agaaatgcag acctagcaac acggaaaaca
      721 atagatattc aaact
//
LOCUS       DAVHIU010000230          735 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVHIU010000230 DAVHIU010000000
VERSION     DAVHIU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.232"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            117..485
                     /locus_tag="VVN06_002076"
     CDS             117..485
                     /locus_tag="VVN06_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU5563540.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGCNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALTVTGFSLG
                     GAIAYGVDWADGSLDGYIFA"
BASE COUNT          239 a           82 c          159 g          255 t
ORIGIN      
        1 ttaaatattt aaaataagat aaaggaagga agtctaattt aggtaactaa acaaagcctg
       61 cgatcacttg ttaggaaacc atcctaacca tgcttaataa aaaaaggaga aaataaatgg
      121 aactcgtatt accaaataat tatgttgctc ttgagcaaga agagatgatg taccttgatg
      181 ggggtggtgt tggttgtaac tggtggaata gtagaggtag ttttgcaaca gttctggatg
      241 tagatttggc catctatagt ggtggtgcaa caatttattc tgcttatgcg ataaaaaaag
      301 ctatctcagc taatagaggg gctattacga gaacattacg tagtttaata attaaacatg
      361 taggtagtgc agctggccat ttagtcaata ctgcactaac tgttactgga ttttcactag
      421 gtggagcaat cgcatatggg gttgattggg ctgacggtag cttagatggt tatatttttg
      481 cttaaaatga aacatttttt gcaggaattg gtgaaataaa ctttgtaagt tatttattgt
      541 atatatgtgt aggaatagcc cctttttttc acgtatatat aataggttct gaaatgaatt
      601 ttgtgaaaat agtgttatct attttgggaa ttatatttgt ttgtatttta actattgctc
      661 gaatttatag gacttttttc tataaagaat agtttggact tgaaatgtat ttaaaaacaa
      721 gaggttggta gatta
//
LOCUS       DAVHIU010000231          734 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVHIU010000231 DAVHIU010000000
VERSION     DAVHIU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.233"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..559
                     /locus_tag="VVN06_002077"
     CDS             <1..559
                     /locus_tag="VVN06_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5563541.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..>734
                     /locus_tag="VVN06_002078"
     CDS             616..>734
                     /locus_tag="VVN06_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEU5563542.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDL"
BASE COUNT          242 a          119 c          138 g          235 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatt
//
LOCUS       DAVHIU010000232          717 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVHIU010000232 DAVHIU010000000
VERSION     DAVHIU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.234"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(106..>717)
                     /locus_tag="VVN06_002079"
     CDS             complement(106..>717)
                     /locus_tag="VVN06_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU5563543.1"
                     /translation="MPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYT
                     LEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKP
                     EEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQD
                     PIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          155 a          150 c          133 g          279 t
ORIGIN      
        1 cacattttca tgaaaaaaat gagaactaga actcacattc tgctctcatt tttcgttttc
       61 ccgttctcct atcctgtttt taggagttag aaaatgctgc tacctttact tactctcctt
      121 taataaagcc aatagttttt cagcttctgc cataatagta ttgttgtcct gggtgccaaa
      181 tagtaaatta ttttttaatc ctgtgagagt ctctttggca ttggacttga taattggatc
      241 ctggattttt ccaagtaaat cttcagcctc tctcagtttt tcttcaacct tttcagtctc
      301 gacctgaggt tcttctgatt cctctggtga ttcttcttct ggttcctctg ttggttttgg
      361 agactctggt ttctcgcttt gcggtttctc ttctcgaggg gtttcttcct caggtttttc
      421 tgtctgaggt ttctcctcgt ttggtttctc cgtttgattg gtatcagctt gaccattttt
      481 gtttctttga acatggtcgc tagcgttacc aaaaccatta tctgaatgcg gacgttcgtt
      541 tggatgttcg acatagtact tgacagtcgc caaaagatcc tcaagagtat accccttagg
      601 tgcctcataa aggccttcgt caaaccactc aaatttgatg ttatggtaat ggtcataatg
      661 aggtatgatt aaactaccgt ttttgacttc tacagtatat tgaagattgt aaggcat
//
LOCUS       DAVHIU010000233          709 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVHIU010000233 DAVHIU010000000
VERSION     DAVHIU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.235"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(91..336)
                     /locus_tag="VVN06_002080"
     CDS             complement(91..336)
                     /locus_tag="VVN06_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563544.1"
                     /translation="MDIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSTSPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            complement(317..679)
                     /locus_tag="VVN06_002081"
     CDS             complement(317..679)
                     /locus_tag="VVN06_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5563545.1"
                     /translation="MELVLPNNYVVLEQEEMIYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYLWA"
BASE COUNT          249 a          155 c           82 g          223 t
ORIGIN      
        1 gataaagcaa agctttaaac ccttccccag acgttagctt ttattcaacc cactacagtt
       61 gacaaggagc ccaaattatt gataaaaact ttagtcatca tcgtctctat aaaaatattt
      121 tctatacact cttgataata ccaaaattaa tgccaatatc ataccaagag ctataataat
      181 agaatttatt agatttattt tagtatatcc aatcactagt ccaataattg gactggtaga
      241 catgcttaga tataataaaa ataacaaaaa atgaatttca ccaattcctg caaaaaaatg
      301 tttcatagct atagttttac gcccataaat atccatcaaa tttaccgtct acgatatcta
      361 aaccatatgc aatggctcct cctaatgaga agacttcagc aacagtagcc gcaatattaa
      421 gagcattagt aatagttgcc atactaactc caccaatata tcttgttata gcacttctaa
      481 cgactcggct catcatacca cgattagcaa ttaacttttt aatggcatat gtctggtata
      541 ctgacatccc tccagaaact gcagctgcag caatattatc acgtttgttc caccagttac
      601 aaccaacacc acccccatca agatatatca tctcttcttg ctcaagaaca acataattat
      661 ttggtaatac gagttccatt tattttctcc ttttttatta agcatagtt
//
LOCUS       DAVHIU010000234          701 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVHIU010000234 DAVHIU010000000
VERSION     DAVHIU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.236"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..282)
                     /locus_tag="VVN06_002082"
     CDS             complement(<1..282)
                     /locus_tag="VVN06_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU5563546.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAK"
     gene            complement(292..>701)
                     /locus_tag="VVN06_002083"
     CDS             complement(292..>701)
                     /locus_tag="VVN06_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563547.1"
                     /translation="ICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSSVV
                     FSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVT
                     KKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
BASE COUNT          209 a          142 c           98 g          252 t
ORIGIN      
        1 ctttgctact tctaataatg tcatagtggt cattaaatga tcttgagcat gtaccatgat
       61 aatttcaatt ttaatttcca ctccacttgc gtattcttgc aagagtttgg tttgtgcatg
      121 atgcgcttca agaattatct catttgattg atttaattta ctttctgcat catcaaaact
      181 accttctctc atttttgcaa atgcttcatg tatttctgac cttgcatttc ccgaatgcag
      241 gataatttca aatgctgcaa cctgcagttc ctcttgattc atatgaacct cctattttat
      301 cttctcaaat atgttaataa aatcttcaaa gttattgcaa gatattagct gattttgcaa
      361 ttcatcattc tctgtcagag agactatctt tttagtcaca gttgccaaac cttcgttccc
      421 atatattgat ggagatagaa gaaatactag ctggacatgt gaactttgat tatcccagag
      481 taacgaatct ttacaaattg caaccgaaac ctttccctct gtaccaaagg gctgaatagg
      541 atgcggaact gcaatttttt cagaaaaaac aactgaactt aattcttcgc gctgtttaat
      601 tccataaagt aaagattgtt caaactcatt tgattcacca acagataaac cctcaaccat
      661 cttttcaagt aaatttacct tgtctgattc agtacatatt a
//
LOCUS       DAVHIU010000235          665 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVHIU010000235 DAVHIU010000000
VERSION     DAVHIU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.237"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..648)
                     /locus_tag="VVN06_002084"
     CDS             complement(<1..648)
                     /locus_tag="VVN06_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU5563548.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKF"
BASE COUNT          186 a          130 c          115 g          234 t
ORIGIN      
        1 aaacttattg aacttactat gatctgcgag taaatatttt ttattagaat tatttaaagc
       61 gatgcgttga gcctctccct cttcctcgct aaaagtagct agagctccgt tttgaatacc
      121 attacagcta acgaaagctt tagaaaattg gagattagag agattttgta gggtcaatgt
      181 gccaacaaaa gcacctgtaa tatcgcgata atttccacct attaaaatca aatctgttaa
      241 ttttcgttcg cttaaaatca gaaaaacagg tagactgttg gttacgacgc ggatattgtc
      301 aataggcaac tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga aaatagtttc
      361 tctttcttct actagactgc ctgcaaaatg ggctatttct tgtttttctg ccgtttggag
      421 ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg gagttggttc gaagtttttc
      481 agctccacca tgcacacgaa tcagcaaatc tttatcagct aattcctgta aatagcgcct
      541 tgcagtcata tctgaaacgg ctatttcgtc cataatctgt ttaaccgtta tggttccttt
      601 gctatttact atctctaaaa ttttggttaa tttttcttgt ttgagcatat tatcacctcg
      661 tttcc
//
LOCUS       DAVHIU010000236          659 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVHIU010000236 DAVHIU010000000
VERSION     DAVHIU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.238"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>659
                     /locus_tag="VVN06_002085"
     CDS             <1..>659
                     /locus_tag="VVN06_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5563549.1"
                     /translation="DSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDI
                     DLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQIL
                     IDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCT
                     DFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKEAPILILDEITASLDVNNEKK
                     I"
BASE COUNT          254 a           86 c          141 g          178 t
ORIGIN      
        1 agactcttta gatgcatcta aagagggctt gatggaaata ttttatttat cgcccaaaat
       61 agaaagatta aaagaaattc aaaatcaaga tttacaagaa ggcgatgact atagcttaaa
      121 aaaatttgat attgatctaa aagatgttga gtttgcctac aataaagacg caaaagtttt
      181 aaacggtgta agttttaaag ctaagcaggg agaggtcact gctttggtag gtgcaagtgg
      241 ctgcggtaaa acaactatct tgaaacttat atcaagactt tatgattatg acaagggaca
      301 aatcttaatt gatggcaaag atataaagga aatatcaaca gaatcccttt ttgataaggt
      361 gtctattgtt ttccaagatg tggttctctt taatcaaagc gttatggaaa atattagaat
      421 cggtaagcaa gatgcaagtg acgaagaggt taaaagagca gcaaaacttg caaattgcac
      481 agattttata gaaaaaatgg ataaaggttt cgatacagtt attggtgaga acggagctga
      541 gctatcagga ggagaaagac aaagattatc aatagccaga gccttcttaa aagaagcgcc
      601 gatattgatc ttagatgaga taacagcaag ccttgatgtt aacaacgaga aaaagattc
//
LOCUS       DAVHIU010000237          650 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVHIU010000237 DAVHIU010000000
VERSION     DAVHIU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.239"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            60..>650
                     /locus_tag="VVN06_002086"
     CDS             60..>650
                     /locus_tag="VVN06_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5563550.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKG"
BASE COUNT          231 a          100 c          133 g          186 t
ORIGIN      
        1 ttattaagag tgtcacaatt gttgtaacta aaataattac aacaagagag gtataaaaaa
       61 tgaaactagc agttattgca gcaaatggac aagcggggaa ggcaattgtt gaagaagcag
      121 tcaaacgagg acatgaagta acggctattg ttcgttctga aaacaaaagt caagctgagt
      181 ctattattaa aaaagattta tttgagttga caaaagatga tcttactgga tttgatgctg
      241 ttatttcagc gtttggtgct tatacaccag atacgctacc gctccacagc aaatcaatcg
      301 aactatttaa tcagcttctt gctggaacac agacacgttt tctagttgta gggggatcag
      361 gaagctttta tatagatgaa acaaaaacga cacgactttt agatacgtct gattttcctg
      421 aagaatttaa gccactagca aaagctcaag cggatgaatt ggatcttctt cgtacaaaaa
      481 ataatctcaa ctggactttt gtcagtcctg ctgtagattt tattcccgat ggtgaaaaaa
      541 caggaaacta cattctagca ggagaaattt ttactactaa tgaaaaagga ataagccaga
      601 ttagttatgc agattatgct attggattgg tagatgaact tgaaaaaggt
//
LOCUS       DAVHIU010000238          636 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVHIU010000238 DAVHIU010000000
VERSION     DAVHIU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.240"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..323
                     /locus_tag="VVN06_002087"
     CDS             <1..323
                     /locus_tag="VVN06_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5563551.1"
                     /translation="FPISPEEFLTTITESLNEYNPKDNYEVLNALALKTSNILFWLDI
                     VFQKTSSEFYIVFEDKEFNQEYYNKLYKKFENLFEEIKSLALQEAKKDKENLDDFALL
                     WGID"
     gene            484..>636
                     /locus_tag="VVN06_002088"
     CDS             484..>636
                     /locus_tag="VVN06_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5563552.1"
                     /translation="MRKYDIPKLLLSGENQGVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVS"
BASE COUNT          237 a          106 c          106 g          187 t
ORIGIN      
        1 atttcccaat cagtcctgaa gagtttctca ctactatcac tgaatcatta aatgaataca
       61 atcccaaaga caactatgaa gttttaaatg cccttgcgct aaagacaagt aatatcctat
      121 tctggctaga tattgtattt caaaaaacaa gtagtgaatt ctacatagtt tttgaagata
      181 aggaattcaa ccaagaatat tacaacaaat tatataaaaa gtttgaaaat ctatttgaag
      241 aaattaagtc tttagctctt caagaagcta agaaagataa agaaaatcta gatgattttg
      301 cgttactttg gggtatagac taacccacgc gccacaatgt tcaacaatcc tgaagcgcgt
      361 gtgcgaatgc gtgtgcgaat aatctacttt caaaaaaacc tttaatagca ctgttagcta
      421 actaaaaata aactattttt cccgcgcgtg tgcgaatgcg tgtgcaaatt gattggagaa
      481 aaaatgagaa aatacgatat accaaaattg ttattatcag gggagaacca aggtgttgaa
      541 ttcaaagaag ctaaaaattc tttccctaaa gacggtatga aaacaatttg ttccttcgct
      601 aatacaaata atggtctgtt aattctaggt gtctca
//
LOCUS       DAVHIU010000239          619 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVHIU010000239 DAVHIU010000000
VERSION     DAVHIU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.241"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>619
                     /locus_tag="VVN06_002089"
     CDS             <1..>619
                     /locus_tag="VVN06_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5563553.1"
                     /translation="MAIGQYLFNPHNTPDNFIFLHAGLGIYTSFFTKEKIGASKNPYI
                     GEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFKNSQLTVLKSLVKTEKDI
                     HLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPADKIYINSQLLNYQPFTHE
                     VRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVAFFIEH"
BASE COUNT          210 a          128 c           89 g          192 t
ORIGIN      
        1 atggctatag gacaatacct ttttaatcca cacaataccc ccgataactt tattttccta
       61 cacgctggat taggtattta cacttccttt ttcacaaaag aaaaaatagg agcctctaaa
      121 aatccttata tcggagaaat tggacacacc attgtcgaat tgaatgggca atattgtgaa
      181 tgcggaaaaa aaggttgttt acaaacatat atttcggatg cttggttaat caaacacgcc
      241 caattattat ttaaaaattc ccaactaact gtactaaaaa gccttgtaaa gactgaaaaa
      301 gacattcatt tagacaccct tttaacggct tataatttag gcgactccgc tttacgccaa
      361 caaattgata aaggagtcaa tttattggcc acttctattg caaatcttct cctcatcaat
      421 cctgctgata aaatctatat caacagtcaa ttgcttaatt atcaaccttt cactcatgaa
      481 gtcagggata aaatccaaga ccagctccac ttcgttccct ttactcgtaa tatagaaatt
      541 gaaattttac cttacaacaa acatcgtgga agtataggag cttgtgcatt agctatcgtt
      601 gcttttttca tagagcata
//
LOCUS       DAVHIU010000240          605 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVHIU010000240 DAVHIU010000000
VERSION     DAVHIU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.242"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            218..598
                     /locus_tag="VVN06_002090"
     CDS             218..598
                     /locus_tag="VVN06_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU5563554.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
BASE COUNT          188 a           86 c          134 g          197 t
ORIGIN      
        1 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
       61 agttaaggag taatgatgaa aaaaagaaca atactattat tgatggccag tctgttagct
      121 cttgtcttag gagcatgtgg tttcttggac atattgatcc tggatcattc tcatcaggat
      181 tactctttac tgctatttta gaaactgggg tggtttgatg gaaagtattg gtattggtct
      241 tgttatcgtt tcacattcca aacacattgc ggaaggtgtt gttgaactga ttagtaaagt
      301 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      361 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgtattttt
      421 tgacctaggt tctgctaaaa tgaacttaaa aatggtgact gatttcagtg ataaaagtat
      481 catcatcaac agggttccaa ttgtagaagg tgcctataat gcagctgctc ttcttcaggc
      541 tggtgcagaa ctgtcagtta ttcaaataca gttagcggag cttgaaatca ataaataagg
      601 aattt
//
LOCUS       DAVHIU010000241          595 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVHIU010000241 DAVHIU010000000
VERSION     DAVHIU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.243"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..>595)
                     /locus_tag="VVN06_002091"
     CDS             complement(<1..>595)
                     /locus_tag="VVN06_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU5563555.1"
                     /translation="LIFISKTLISGGRQDIIKILIAYVIMMYIQQKRKVGWNRVISHK
                     YIHLGFVGLIAGIPAFYYSLFLAGRSTTRTLFESVSTYLGGSIQHFNQYIENPLDPGE
                     VFGSETLVPILNILGEMGLVNYRSTIHLEFRTLGVTVGNVYTFFRRPLHDFGLVGMYV
                     FVFAVGAFFAIYYLVLRKKQVGFNLDIHTIIYSYVFY"
BASE COUNT          234 a          113 c           78 g          170 t
ORIGIN      
        1 caataaaaga cataagaata aataatggta tgaatatcca aattaaaacc aacctgtttc
       61 tttctcagaa ctaaataata aatagcaaaa aaagcaccta cagcaaagac aaatacatac
      121 ataccaacta gaccaaaatc atgcaagggt cttctaaaaa aagtataaac atttcctaca
      181 gtaactccta gtgtccgaaa ttctaaatga attgtactac gataattaac taggcccatt
      241 tcccctaata tatttaatat aggcaccaat gtttcactgc caaaaacttc accaggatct
      301 aatggatttt caatatactg attaaaatgc tgaattgagc ctcctagata ggtcgaaaca
      361 ctctcaaata gcgtcctagt cgttgaacga ccggctaaaa acaaagagta gtaaaatgct
      421 ggaatacctg ctattaaacc aacaaatcca aggtgaatat atttatgaga tatgactcta
      481 ttccatccaa ctttccgttt ttgttggata tacatcatga ttacataggc aatcagaatt
      541 ttaataatat cttgccggcc tcctgatatt aaagtttttg aaataaatat taata
//
LOCUS       DAVHIU010000242          575 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVHIU010000242 DAVHIU010000000
VERSION     DAVHIU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.244"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
BASE COUNT          165 a          101 c           90 g          219 t
ORIGIN      
        1 gttgtttatt acctttcttg taataagctt gttacctgat aagatttttg gaaaaaataa
       61 aaaaatttgg aaaatagttt ttgcaatatt gacggcagtg gcagcattgt catttatgta
      121 ctaagttatt ttaagaatgt agggaaataa accctacatt ctttttagtt ttttctgttt
      181 tctaaattct atttatccaa gcgcttcaac atttcttgct tcttcgcttc aagttctgca
      241 cgcttttctt cgatttcggc atgttttttc tcgagttcag aacaacttgc accattgcta
      301 aattcttttc gccatcagga gatagggtga gtcgacatgt ctattactca cccaaagcag
      361 tcctacaaag caggaatttt ctgttacttt tttggaaata gtaacgttta tacagctttg
      421 acacttcgta tcaaagcgcc aaacacactc cgagggattt acagaaagca gaaaaggaat
      481 gatctggtat aagatcattc cttttctctc ttttttattt gtatctgctt acattttatc
      541 atatactgtt attatagtca aaaaaatatg ctatt
//
LOCUS       DAVHIU010000243          541 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVHIU010000243 DAVHIU010000000
VERSION     DAVHIU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.245"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            1..541
                     /locus_tag="VVN06_002092"
                     /pseudo
     CDS             1..541
                     /locus_tag="VVN06_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          186 a           99 c          103 g          153 t
ORIGIN      
        1 gctacactcg aaaaaaagaa ccacacctac tatgaacaag acccagaaaa agtagcctta
       61 tttcttaaga attttaatag tttaaagcac ctagcacctg tttagattga cgaaacagga
      121 ttcgatactt atttttatcg agaatatggt cgctcattaa aaggtcagtt aataagaggc
      181 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac caatggtgag
      241 ttaatcgctc caatgactta cgaagagacg atgacgagcg acttttttga agcttagttt
      301 cagaagtttt tcttaccaac attaaccaca ccatcggtta ttattatgga taatgtaaga
      361 ttccatagaa tggggaagaa tgtaagattc catagaatgg ggaagctaga acttttatgc
      421 gaagagtttg ggcataaact tttacctctt cctccctact cgcctgagta caatcctatt
      481 gagaaaacat gggctcatat caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc
      541 t
//
LOCUS       DAVHIU010000244          537 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVHIU010000244 DAVHIU010000000
VERSION     DAVHIU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.246"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            370..>537
                     /locus_tag="VVN06_002093"
     CDS             370..>537
                     /locus_tag="VVN06_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU5563556.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIIL"
BASE COUNT          232 a           84 c           95 g          126 t
ORIGIN      
        1 tagaagaaag aatagatgat aatgtagaaa aatcatctaa agatgaaata acataattgt
       61 cccttggtta caagggcgca attatacacc ctaaagggtg cttgcgtcct gcggagtcaa
      121 acccttgcag ggagcaacaa ccattcgctt tttaaaagtc aagggtaaat aaactcgcta
      181 aggctcgccc ttgactttta aaatttgaaa tgaaagtaac aattaagccg acatactgaa
      241 acaacaaaaa ttaattcagt agtcggcttt tttaattcta tcatttcaaa acgctcattc
      301 acaaagagga tataaaaaaa ctattaagcc tccacctaaa acaacaaaga aagaaaggaa
      361 gtgataaaaa tggcagacag ttttcatttt tcagtaaaca taatatcaag aggaaaaggc
      421 aaaagtgcag tagcaagtgc agcatatata agtggagaaa aaataaaaaa tgaatgggac
      481 ggagtaaccc atgactacac aagaaaagaa aaaatattag taaaaaatat aatattg
//
LOCUS       DAVHIU010000245          528 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVHIU010000245 DAVHIU010000000
VERSION     DAVHIU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.247"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>528
                     /locus_tag="VVN06_002094"
     CDS             <1..>528
                     /locus_tag="VVN06_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563557.1"
                     /translation="IKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVAD
                     LVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALAL
                     AQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFA
                     IKEGKLVAEGKPEDIL"
BASE COUNT          198 a           95 c           91 g          144 t
ORIGIN      
        1 tataaagcca ttagagggag aagtattgct cgataataaa tcaattaatt cttataaaga
       61 aaaagattta gcaaaacaca tagctatatt acctcaatct ccaataatcc ctgaatcaat
      121 aacagtagct gatcttgtaa gccgtggtcg tttcccctac agaaagcctt ttaagagtct
      181 tggaaaagat gaccttgaaa taataaacag atcaatggtt aaggccaatg ttgaagatct
      241 agcaaataac ctagttgaag aactttctgg gggtcaaagg caaagagtat ggatagctct
      301 agccctagcc caagatacaa gtatcctact tttagatgag ccaactactt acttggatat
      361 ctcatatcaa atagaactat tagacctctt gactgatcta aaccaaaaat ataagacaac
      421 catttgcatg attttgcacg atataaatct aacagcaaga tacgctgatt acctatttgc
      481 aattaaagaa ggtaaacttg ttgcagaggg aaagcctgaa gatatact
//
LOCUS       DAVHIU010000246          527 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVHIU010000246 DAVHIU010000000
VERSION     DAVHIU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.248"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..192)
                     /locus_tag="VVN06_002095"
     CDS             complement(<1..192)
                     /locus_tag="VVN06_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563558.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYG"
     gene            complement(238..444)
                     /locus_tag="VVN06_002096"
     CDS             complement(238..444)
                     /locus_tag="VVN06_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563559.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(431..>527)
                     /locus_tag="VVN06_002097"
     CDS             complement(431..>527)
                     /locus_tag="VVN06_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5563560.1"
                     /translation="TQDKIAEYLSLNQSMIAKMEKGERNITNGFK"
BASE COUNT          166 a          116 c           62 g          183 t
ORIGIN      
        1 accataaaac tgacccaatt ttataatatc ttctacttca agatgagttc tattggcatt
       61 ttctctaatt tcctcaacca tcctatacag tgaagatggg aaaattaaaa aataagaagc
      121 aaactgatcc gcttttcttt cagtttcatc tccttcacca atcaagataa gactgactga
      181 actcttcttc acctcatcat aataaagatg atacagttca tgtgctaaag aaaatcttta
      241 ccttcctaat gacatgtctg aattgactgc aatgagactg aactgagttc ctttataaca
      301 gactccgcta atattctttc cgagtccata aaataccagc gtcaaatttt ctatcttttg
      361 taccaattta aaaatatcta tcggcgattc accatcagct cccaactttt ttctaagatt
      421 tggagcttta ttacttaaat ccattcgtga tattcctttc acctttttcc attttggcaa
      481 tcatgctttg attcaaagac aaatactcag caatcttatc ttgagta
//
LOCUS       DAVHIU010000247          526 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVHIU010000247 DAVHIU010000000
VERSION     DAVHIU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.249"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>526
                     /locus_tag="VVN06_002098"
     CDS             <1..>526
                     /locus_tag="VVN06_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563561.1"
                     /translation="LIYKFLDKLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSH
                     ILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQ
                     AIVTPILVLVLGFIVSIRVGITLLALTIIGGLILGAMMGEQEFMKIYQEALSKLSAET
                     VEYVRGMQVVKIFKA"
BASE COUNT          177 a           77 c          107 g          165 t
ORIGIN      
        1 tttaatctac aaatttctag ataagttaat aattaattca aatttatccg gtgcagagag
       61 catagcatta aaatctgtta ttacactaac aagtggagcg atattttatt ttgtctcagg
      121 aatgttttca catatcttgg gattcaggct tgaaacaaat ttaagaaaaa ggggaatcga
      181 tggtctggaa aaagcaagtt ttaggttctt tgacttaaat ccatctggtc aaataagaaa
      241 gatcatagat gataatgcag cacaaactca tcaggtggta gctcacatga ttcccgatag
      301 ctctcaggca atagtcacac ctatacttgt ccttgtacta ggctttatag taagtataag
      361 ggttggtata actttgcttg ctcttactat aattggtggc ttaattttag gggcgatgat
      421 gggcgagcaa gaatttatga agatatacca agaagctcta tctaaactaa gtgctgaaac
      481 tgttgagtac gtgagaggaa tgcaagttgt aaaaatattt aaagca
//
LOCUS       DAVHIU010000248          519 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVHIU010000248 DAVHIU010000000
VERSION     DAVHIU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.250"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..>519)
                     /locus_tag="VVN06_002099"
     CDS             complement(<1..>519)
                     /locus_tag="VVN06_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5563562.1"
                     /translation="KDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLS
                     GSLSFTKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIF
                     CAVSLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNIL
                     PARYPVGVVTGLL"
BASE COUNT          191 a           95 c           77 g          156 t
ORIGIN      
        1 taacaagcca gttacaacac ctactggata tctagctggt aaaatatttt gagagaatat
       61 gtctgataac aaaactagta aaattccaac caatccagct aatattgggc ttcttttctt
      121 gccaatattt aaggctatgg gaccagctaa aaaagatata caagctattg gtcctgtaat
      181 tgaagtagaa aaagcagtta aagatacagc gcaaaaaatt aaaacaagcc ttgaaagctc
      241 gggatttgct ccaagtccga ttgctatttc ttcaccaagt tcaataattt ctagtctttt
      301 attaaaaaat aaaactaata tagtagcaat aatacttact attagaacaa gaggtatgtc
      361 atctaacttt gtaaaagata aagagccact gagccatctc ataacttctt gtaattcata
      421 tcttgctact ttcaacaata aaaatgaggt gcctgctctt gtgacagctt gaaaaccaat
      481 acctaatatt atcagtcttg ctgctgaaaa accatcttt
//
LOCUS       DAVHIU010000249          519 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVHIU010000249 DAVHIU010000000
VERSION     DAVHIU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.251"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            33..>519
                     /locus_tag="VVN06_002100"
     CDS             33..>519
                     /locus_tag="VVN06_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5563563.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     IT"
BASE COUNT          196 a           96 c           83 g          144 t
ORIGIN      
        1 tttaatataa aataaaccaa aggaggaaac aaatgattac aaaacaatca gcctttttac
       61 aaaatagagc cactatactg gagtttctat atcgaaatcc agcaacatcc cgtacagata
      121 ttgttaatga aacaggactt actccagcaa caacgactaa catcataaaa gagctaagcg
      181 aacagtccct catatacgaa actggtgatg aatttagcga gttttccgga tctggaagac
      241 gtagaaaaac tatttctatc acagataata ttccgtatgt tgttggaggt attgaaataa
      301 acgtcctagg tatctttctc agtctatgtg acttacaagg gaaaactctt ttcgagacag
      361 aaattttgaa tgaagattat cctatttcag aaatcaattc caccattacc aatatgataa
      421 aaacagctat agagtacgtc cctttggaaa caaaattact tggatttggc ttatcaatac
      481 ctggacatta taacaaagac tccggaagta tcattacaa
//
LOCUS       DAVHIU010000250          514 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVHIU010000250 DAVHIU010000000
VERSION     DAVHIU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.252"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            108..>514
                     /locus_tag="VVN06_002101"
     CDS             108..>514
                     /locus_tag="VVN06_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563564.1"
                     /translation="MAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVLLLF
                     MFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQSLSKFILDFKESLDNIRVGI
                     NLMLSNIASSMIIGIVRMGIQWNWNIETFGKVS"
BASE COUNT          147 a           74 c           95 g          198 t
ORIGIN      
        1 attctcagat gattctattt tctagtttct taatgctaat ctcgctggta ttatttactt
       61 tgaacctaat aactgtaagg gatgaaaacg caagatatat ttataatatg gctatcatta
      121 gcatgatagt cacaaactta agaacactct atgtttatat cttgcagatg acaaatcgct
      181 tgaaggatag ttcagtcatt ctaattagtg atcgcgtttt atatgtactc cttttattca
      241 tgtttattgt atttggatgg catgagtaca aggtcatgat ttgggctgat attctaggtc
      301 gaacattttc tctcatgctt tccttctgga tttgtaaaga tattgtgttt cagtctttgt
      361 caaaatttat cttagatttc aaggagtccc ttgataatat ccgtgttgga atcaatttaa
      421 tgctatctaa cattgcgagt agcatgatta taggcattgt tcgtatggga attcaatgga
      481 attggaatat tgaaacattc gggaaagtgt catt
//
LOCUS       DAVHIU010000251          505 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVHIU010000251 DAVHIU010000000
VERSION     DAVHIU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.253"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            128..427
                     /locus_tag="VVN06_002102"
     CDS             128..427
                     /locus_tag="VVN06_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5563565.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          183 a           95 c           98 g          129 t
ORIGIN      
        1 aaatggtata ataaaatcaa tacttgggct tgatggctat gctactaata acaattagga
       61 gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa ggaactttag
      121 aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc aaaaacaaat
      181 ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg ctcaagatct
      241 tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca atccagctgt
      301 cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca tcaactctgc
      361 tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt gctttcgctg ggaaattacc
      421 tttctaaaaa caaggagcta gaacaatgct tgtcgataac aaaggctaag aaaggtgcaa
      481 aaatgactct ataatatttg tagtg
//
LOCUS       DAVHIU010000252          497 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVHIU010000252 DAVHIU010000000
VERSION     DAVHIU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.254"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(324..>497)
                     /locus_tag="VVN06_002103"
     CDS             complement(324..>497)
                     /locus_tag="VVN06_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU5563566.1"
                     /translation="SEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEWDT
                     ISSDSDFMPFQQS"
BASE COUNT          173 a           91 c           70 g          163 t
ORIGIN      
        1 atccttttta agctatctaa acacattaaa gtcatcaagc cttaataaac tgaaagagcc
       61 tgggtaacaa ctcaatttca gaagaaaaaa agtacatctt cccacaataa aacgcatagt
      121 atcaggtgtt gaaacacctg atactatgcg tttttctata tcaaagactt tttgcccaat
      181 tcctcaaagc tatacattga gaaatctgcg actcgcttcc tagtttaaag ttgctctttg
      241 attttcattg agtataagca acgtgattag ataagagttt tacagagtac taaatataag
      301 atttaacttt tttgagaaat tggttacgat tgttgaaacg gcataaaatc tgaatcagag
      361 gagatagtat cccattcttc aggattgata aaatttaaaa atagattttt aaattcttcc
      421 agctcatcat ctgaatgaca aatccattct ttaccagtcg agacatacca ttttccaaga
      481 cttttcaatt cttcact
//
LOCUS       DAVHIU010000253          497 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVHIU010000253 DAVHIU010000000
VERSION     DAVHIU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.255"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>497
                     /locus_tag="VVN06_002104"
     CDS             <1..>497
                     /locus_tag="VVN06_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEU5563567.1"
                     /translation="VEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVT
                     EVASQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIE
                     DRAFDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLE
                     KLTLP"
BASE COUNT          183 a           76 c           87 g          151 t
ORIGIN      
        1 ctgtagaagg taacatagga actgttcaag tgggtaatgg agttactcct gtagagtttg
       61 aagctggtca agatggaaaa ccattcacga ttcctacaaa aatcacagta ggtgataaag
      121 tatttaccgt tactgaagta gctagtcaag cttttagtta ttatccagat gaaacaggta
      181 gaattgtcta ctatcctagc tctattacta tcccatcaag cataaaaaaa atacaaaaaa
      241 aaggcttcca tggaagtaaa gctaaaacta ttatttttga caaaggcagt cagctggaga
      301 aaattgaaga tagagctttt gatttttctg aattagaaga gattgaattg cctgcatctc
      361 tagaatatat tggaacaagt gcattttctt ttagtcaaaa attgaaaaag ctaacctttt
      421 cctcaagttc aaaattagaa ttaatatcac atgaggcttt tgctaattta tcaaatttag
      481 agaaactaac attacca
//
LOCUS       DAVHIU010000254          492 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVHIU010000254 DAVHIU010000000
VERSION     DAVHIU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.256"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            103..>492
                     /locus_tag="VVN06_002105"
     CDS             103..>492
                     /locus_tag="VVN06_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563568.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQ"
BASE COUNT          186 a           60 c          100 g          146 t
ORIGIN      
        1 atgagccttt tgaaagcagt ataaaattgg tatcaatcaa tgagtaacaa agtatcagca
       61 tgattcgaaa catttacttg taatttgcgg aggaacatca atatggatgg aataacaaaa
      121 gattttataa aaacggctaa actaatgaaa caattatggc cccaattgac cgataaagaa
      181 gctattgatg aagtaaaaaa atatacgaat ggcaaaaata ctgcaatctt tactgaagtt
      241 gaaggtgaca caattgtagg tctagcacta tgttcactca gatttgatta tgttgaaggt
      301 tgtaaatata gtcctgttgg attcttagaa gggattattg ttgacgagga atatcgttta
      361 aaggatattg ctaaaaatct ctgtacaaaa tgtgaggaat gggcgaaaaa taaaggatgt
      421 aaggaatttg caagtgactg tactttaacg aatacggatt ctataagatt tcatctcaat
      481 attggatttc ag
//
LOCUS       DAVHIU010000255          491 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVHIU010000255 DAVHIU010000000
VERSION     DAVHIU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.257"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>491
                     /locus_tag="VVN06_002106"
     CDS             <1..>491
                     /locus_tag="VVN06_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563569.1"
                     /translation="IYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKTDMLEGNGVYS
                     KEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFNIIRYQVFTFKM
                     NDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLFIDTKPVNLS
                     MDK"
BASE COUNT          196 a           67 c           96 g          132 t
ORIGIN      
        1 atctattctg attcagaaaa acaacaatat ctgttaaagg ataatatcat tctaagaaaa
       61 ggctatgcac taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat
      121 ggagtctata gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagtc
      181 ctttcagaaa caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa
      241 gctgtattaa tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt
      301 aaaatgaacg ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct
      361 aaacttgtat tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta
      421 gaagctataa aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca
      481 atggataaga a
//
LOCUS       DAVHIU010000256          485 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVHIU010000256 DAVHIU010000000
VERSION     DAVHIU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.258"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(118..483)
                     /locus_tag="VVN06_002107"
     CDS             complement(118..483)
                     /locus_tag="VVN06_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU5563570.1"
                     /translation="MRLLLGQIPLVVSTYLFLSRQFLNFSLVFQFLLVIINLASILAT
                     VYLTREMRIRKFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAAESYQQIIAYIGA
                     VLCLLIMLLLIWGLKYYKK"
BASE COUNT          186 a           93 c           68 g          138 t
ORIGIN      
        1 gtactattct agtttcaata tactatacta taccatattt tgtttcgcag ggaatctatt
       61 ataaaagggt aagtattgcg aaaacactta cccttttctt ttatacttca ttaagctcta
      121 ctttttataa tacttcaaac cccaaatgag caaaagcatg ataagcaagc agagaaccgc
      181 gccaatataa gcgattattt gttgatagga ttctgctgct gtgatccctc tatacaaaca
      241 tataatagac ataaaacctg tcaagccgat aaacatgagt tgattggttc taggactaac
      301 caaatcatca tcttcaaact ttcttatcct catttcccta gtgagataaa cagtagccaa
      361 aatagaagcc aagttaataa tcactaaaag aaattggaaa accaaggaaa aatttaaaaa
      421 ctgacgagat agaaatagat aagtagaaac aaccaaaggt atctgtccta acaacaacct
      481 cacta
//
LOCUS       DAVHIU010000257          483 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVHIU010000257 DAVHIU010000000
VERSION     DAVHIU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.259"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            5..289
                     /locus_tag="VVN06_002108"
     CDS             5..289
                     /locus_tag="VVN06_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563571.1"
                     /translation="MVWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            282..431
                     /locus_tag="VVN06_002109"
     CDS             282..431
                     /locus_tag="VVN06_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563572.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
BASE COUNT          168 a           83 c           81 g          151 t
ORIGIN      
        1 aacaatggtt tggtacgatt atatgataca ggcatccaaa caatcacaat tcaacgcaag
       61 ccattggttt cgctatttgc gaaaagttat ttttgaagac tattcttatt taacaaacca
      121 agatgtagaa aagttgctag actccaaaga actaacccgt tttcaaaaaa ttagcttgaa
      181 gtatgccttt caagagcata ctccaactca taaatatgtg atttcattaa ataaacctgc
      241 taagttaacc aatgttcaaa aattgatgga gaaatacaaa catggataaa atgaaaccgg
      301 tcttccaagc cctaaataag gaattaattc aggaaaatct gactttaaca attatctgtg
      361 tcgatggtta tgtcttagaa tatcatggtt tacgtgccac acaagatgtt gatgctttta
      421 tggctctata atatttgtag tgggtaaccc cccctatgga tattatggag cctattttgt
      481 gta
//
LOCUS       DAVHIU010000258          483 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVHIU010000258 DAVHIU010000000
VERSION     DAVHIU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.260"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(293..469)
                     /locus_tag="VVN06_002110"
     CDS             complement(293..469)
                     /locus_tag="VVN06_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563573.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
BASE COUNT          159 a          100 c           62 g          162 t
ORIGIN      
        1 agtcaattga aaaaatgtag tggacaaaat atcttttaac aaaccaagag tttattaaag
       61 agttatcact tttcaacttt tctaagctta tgcagttgtg aaacaaacta cttttaaact
      121 attaactaag ataggattga taaataattt caaactctta ctagcaatca tacgatattc
      181 aagctcgcgt gcttttttcc ttcctgctta tttcttagaa ctgaagaacc cggatcggta
      241 tataaattat ccggatcaac atagtcataa gactcataac agttgcgctt cattaagtca
      301 tccccagagc aagagcttca tctcgtaatt tttcaacatc actaaccgta ggtcgccatc
      361 cttcaatcat atttgtactt aaagcatacc aaacactctt aaaaacggat cggttttcaa
      421 aagctattcc catgattgtc atcttttctt tatctatatc taaggacata tgctacctcc
      481 ttt
//
LOCUS       DAVHIU010000259          473 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVHIU010000259 DAVHIU010000000
VERSION     DAVHIU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.261"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(114..467)
                     /locus_tag="VVN06_002111"
     CDS             complement(114..467)
                     /locus_tag="VVN06_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5563574.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
BASE COUNT          158 a          114 c           58 g          143 t
ORIGIN      
        1 agctaaaaaa ataagcaaaa aatactccgt atcatttata atcgccctga atgataaaaa
       61 tatatacacc caacacacga tgatgtgtat ttttcgaatc ttatctacat tactcatctt
      121 actgttgcag atatgaatcc atcccaagga taagggttcc cgttaatatc acaacctcta
      181 ttaagaacac catatccaat acagtaagct attttccctg ctgctgtagc caatacacct
      241 gctgctgcac caataagcca gcctccaaga cctccaacct taatatagtt tattagtttt
      301 ttggttaaaa ctgcagctcc agctaatgct ataaaagttc ctgaactcat agctaaagct
      361 gcgcaaattc cttgacaagc acgcttgctt aaataagctc ccccatcaag atacatcatt
      421 tcttcttcat caatcacaac ataattattt ggtaatacaa gttccatttg ttt
//
LOCUS       DAVHIU010000260          469 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVHIU010000260 DAVHIU010000000
VERSION     DAVHIU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.262"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>469
                     /locus_tag="VVN06_002112"
     CDS             <1..>469
                     /locus_tag="VVN06_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU5563575.1"
                     /translation="HQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAP
                     IRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTH
                     SHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAA"
BASE COUNT          151 a           94 c          109 g          115 t
ORIGIN      
        1 ttcaccaaga tttacttgat aataaaggtc gacaagttga ttttgaggct ttggataacc
       61 tgttggaacg actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg
      121 ccttcttagc tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct
      181 acactgatga tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt
      241 atatctttga tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata
      301 tgacccatag ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc
      361 aggcttatgc taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa
      421 atactgaggc aaaaggagca gaagctatct acaaccgcgt gaaagcagc
//
LOCUS       DAVHIU010000261          462 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVHIU010000261 DAVHIU010000000
VERSION     DAVHIU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.263"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>462
                     /locus_tag="VVN06_002113"
     CDS             <1..>462
                     /locus_tag="VVN06_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5563576.1"
                     /translation="LSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGL
                     EKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGIKNFDEEKAKI
                     ILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFIFFDEPSSGMDYS"
BASE COUNT          173 a           61 c           91 g          137 t
ORIGIN      
        1 atcttagcta caaatttact gatgatgagt gtttaagctt aaaagatatt tcattcaagc
       61 ttgggaaaat ttatggcata ataggatcca acggacgagg aaaatcaacg cttttaagat
      121 gtttaatagg tcttgagaaa aaatcaaaag aagaaattta ttttaaggga gagaagctat
      181 ctaaaaaaga aagactcaaa aactcttcac ttgttatgca agatgtaaat catcaattat
      241 tcacagatga ggtattcaac gagcttagat taggaataaa gaattttgat gaagaaaagg
      301 cgaaaatcat tttaaaagat ttaggcctgg acgaatttat tgaaaggcat ccgatgagtt
      361 tatcaggagg gcaaaagcaa aggcttgcaa tagcatctgt tatgtgcaag acttctccat
      421 ttatcttttt tgacgaacct tcaagtggta tggattattc ca
//
LOCUS       DAVHIU010000262          414 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVHIU010000262 DAVHIU010000000
VERSION     DAVHIU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.264"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..336
                     /locus_tag="VVN06_002114"
     CDS             <1..336
                     /locus_tag="VVN06_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU5563577.1"
                     /translation="EKPKPEVKPQLEKPKPEVKPQLEKPKPEVKPQPEKPKPDNSKPQ
                     ADDKKPSTTNNLSKDKQPSNQASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGA
                     TILAKKRMK"
BASE COUNT          179 a           81 c           73 g           81 t
ORIGIN      
        1 gaaaaaccaa aaccagaggt taaaccgcag ctagaaaaac caaaaccaga ggttaaaccg
       61 cagctggaaa aaccaaaacc agaggttaaa ccgcagccgg aaaaaccaaa accagataat
      121 agcaagccac aagcagatga taagaagcca tcaactacaa ataatttaag caaggacaag
      181 caaccttcta accaagcttc aacaaacgaa aaagcaacaa ataaaccgaa gaagtcattg
      241 ccatcaactg gatctatttc aaatctagca cttgaaattg caggtcttct taccttggcg
      301 ggggcaacca ttcttgctaa gaaaagaatg aaatagtgat tgattcattc aaaaatgttt
      361 gtaaaaagta ttcgacttaa aagaaaagag tctgggataa aaagattttg attt
//
LOCUS       DAVHIU010000263          409 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVHIU010000263 DAVHIU010000000
VERSION     DAVHIU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.265"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VVN06_002115"
     rRNA            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VVN06_002115"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(173..>409)
                     /locus_tag="VVN06_002116"
     rRNA            complement(173..>409)
                     /locus_tag="VVN06_002116"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           99 a          109 c           75 g          126 t
ORIGIN      
        1 cacagggggc aacccccaac tacttccggc gttctagggc ttaacttctg tgttcggcat
       61 gggtacaggt gtatctccta ggctatcgtc acttaactct gagtaatacc tactcaaaat
      121 tgaatatcta ttcaattcaa gaaaaccgtt cgctttcata ttctcagtta ctttggataa
      181 gtcctcgagc tattagtatt agtccgctac atgtgtcgcc acacttccac ttctaaccta
      241 tctacctgat catctctcag ggctcttact gatatataat catgggaaat ctcatcttga
      301 ggtgggtttc acacttagat gctttcagcg tttatccctt ccctacatag ctacccagcg
      361 atgcctttgg caagacaact ggtacaccag cggtaagtcc actctggtc
//
LOCUS       DAVHIU010000264          407 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVHIU010000264 DAVHIU010000000
VERSION     DAVHIU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.266"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..216)
                     /locus_tag="VVN06_002117"
     CDS             complement(<1..216)
                     /locus_tag="VVN06_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563578.1"
                     /translation="MFYNQQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYI"
BASE COUNT          169 a           70 c           61 g          107 t
ORIGIN      
        1 aatgtaagat aatagaaaat aaaacaggac taacaaaatt gtattgacta caatagctac
       61 tataaaatct aatcctaaat cacctggaat acccaattta cttactatga ctagtaacac
      121 aatgccattc aaagcataaa taatggaaac tgagatggca tatagaaaat tagacaagac
      181 gtaaatcatc cgattcttct gttgattata aaacaaactt accgtattgt gggaaaaatc
      241 tctactaatg actcgattag catgtataac agccattaac ataccgagca tagcataaaa
      301 gtttgaaata tgcttaatac caagcgatgt cccttgagtc tttagaataa agaatgtaac
      361 caatatcggc ggtataaacg agataagcaa accgagatat attacgc
//
LOCUS       DAVHIU010000265          389 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVHIU010000265 DAVHIU010000000
VERSION     DAVHIU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.267"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            172..360
                     /locus_tag="VVN06_002118"
     CDS             172..360
                     /locus_tag="VVN06_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563579.1"
                     /translation="MMVGGILWAILGLSYLEPVTTLALGYQKDISLSLVSGHLAFILF
                     VSCLVVYGTFFLHSEDYV"
BASE COUNT           96 a           51 c           84 g          158 t
ORIGIN      
        1 ggagattata gtggtgcctt aaaagaatta tttgattcta atcagtttct ctatagtctt
       61 gtgacgactc tctggtatgg agtttggggc gctgtgttct ctatttttgg actagctagt
      121 gctttgctag tgaagaaaaa attaggagct attttcatcc cagttgccta tatgatggtt
      181 ggtggtattt tatgggctat tttagggcta tcttacttag aacctgtgac aacgctagct
      241 ttgggatatc agaaagatat cagtctttcc ttagttagtg ggcatcttgc ttttatttta
      301 tttgttagtt gtttggttgt ttatggtaca ttttttctac attcagagga ctatgtataa
      361 tgaaacaatt tgttcaattt tataaaaaa
//
LOCUS       DAVHIU010000266          380 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVHIU010000266 DAVHIU010000000
VERSION     DAVHIU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.268"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>380
                     /locus_tag="VVN06_002119"
     CDS             <1..>380
                     /locus_tag="VVN06_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5563580.1"
                     /translation="IYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELIN
                     GFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYY
                     NYCRKYFNLLSFNERILVTKKYYS"
BASE COUNT          138 a           45 c           76 g          121 t
ORIGIN      
        1 atttatccaa atggtacacg agaagaggag aagaccgatt ttgttggaaa tcccttgttt
       61 gttcaaatgg ttcacaatat agcaggtacg tcattttggt tgtgtaaaaa agaggtgcta
      121 gaattaatta atggttttga gaaaatagat tcacatcagg acggtgttgt tttattaaaa
      181 ctacttgctc aaggatacca aattgatata gtgcgagaat tcttggtgaa ttactacgct
      241 cacagtaaag aaaacggtat cactggagtg acacaaaaaa caattaatgc agatgaagaa
      301 tattataatt actgtaggaa atattttaat ttattgagtt tcaacgagag aatattggtt
      361 acaaagaaat attattcttt
//
LOCUS       DAVHIU010000267          380 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVHIU010000267 DAVHIU010000000
VERSION     DAVHIU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.269"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..>380)
                     /locus_tag="VVN06_002120"
     CDS             complement(<1..>380)
                     /locus_tag="VVN06_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563581.1"
                     /translation="VTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNI
                     GYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVD
                     TSYSEAPEDIEGQISTVESTFKQRA"
BASE COUNT          101 a           80 c           62 g          137 t
ORIGIN      
        1 gctcgttgtt taaaggtcga ttctactgtt gaaatctgac cttctatatc ttcaggagct
       61 tctgaataag aagtatctac atcgcttatt tcaaacttcg gcatccaaat ccaaacggtt
      121 ccttcctggt tgaaattgaa caaccattca tttgtggtct gcttggattc gtttgtccaa
      181 ccttttggaa tatggacaac atatcgttta atttctgtcg acaatgtcac atttccagtt
      241 ttatatccga tattccctaa tcgagatctt agcattattc catttttatt tgccttagca
      301 taaaaactaa tggttacatc ttgattagtc gtacttccgg gaattacttt cccgaattga
      361 cccagagctg gataagtaac
//
LOCUS       DAVHIU010000268          374 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVHIU010000268 DAVHIU010000000
VERSION     DAVHIU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.270"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            21..>374
                     /locus_tag="VVN06_002121"
     CDS             21..>374
                     /locus_tag="VVN06_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563582.1"
                     /translation="MDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGF
                     GAAQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKV
                     SVFLKSDEMEMIRKAM"
BASE COUNT          127 a           40 c           84 g          123 t
ORIGIN      
        1 ctagagaaat tcttgcaaat atggacaagt tcttggagtg gacaatatca gatgatgaag
       61 tagcatatgt aagtcttcac tttttagctg ctatggaacg tagcaaagag agtactaagt
      121 ttaatattct tgctatttgt gcaacaggat ttggtgcagc ccaaatgtta agaaatcgtt
      181 tggaaacgga gtttgggaag cgagtagaag ttgttgatgt aataggctat tatgaattga
      241 atcaagaaaa acttaaagga atagatttta tagtgtcagc tgttgattta tcaaatctat
      301 attttcaaat cccagttttt aaagttagtg tgtttcttaa aagtgatgag atggaaatga
      361 ttagaaaagc tatg
//
LOCUS       DAVHIU010000269          371 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVHIU010000269 DAVHIU010000000
VERSION     DAVHIU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.271"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(14..340)
                     /locus_tag="VVN06_002122"
     CDS             complement(14..340)
                     /locus_tag="VVN06_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563583.1"
                     /translation="MMYLDGGFSIPRWPVATAINIAFNGVLGGGAISLVRNYIRNYGL
                     RRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGDAITTIWANNDVNRRDP
                     NLNALW"
BASE COUNT          115 a           98 c           51 g          107 t
ORIGIN      
        1 tacccatatc tagttaccac aaggcgttta aatttgggtc tctcctattt acatcattgt
       61 tggcccagat tgttgtaata gcatcgccaa ttgacatcca agctgcaaat ccattaatag
      121 cagacagtgc aaatcctgcc actctatttg caactcgtac cccaacatat cttgcagctg
      181 ctccagcaat tgcgcttgta actcgccgca aaccataatt acgaatataa tttctaacta
      241 gactgattgc tcctccacct aaaacaccat taaaagctat attaatggct gttgcaacag
      301 gccatctcgg aatagaaaat cccccatcaa gatacatcat ctcttcttgc tcaagaacaa
      361 cataattatt t
//
LOCUS       DAVHIU010000270          325 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVHIU010000270 DAVHIU010000000
VERSION     DAVHIU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.272"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            complement(<1..>325)
                     /locus_tag="VVN06_002123"
     CDS             complement(<1..>325)
                     /locus_tag="VVN06_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5563584.1"
                     /translation="KTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSETL
                     YQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKE
                     MKEGFF"
BASE COUNT           83 a           69 c           79 g           94 t
ORIGIN      
        1 caaaaaatcc ctccttcatc tccttgatga aattctcggc ttgaccacgt ccacgataaa
       61 gctgaaactg gtcttggctt gttccactcg tcatatttgt aacgagagaa ataacatcgt
      121 agaacaagtt tccttctttt cgttcagaga actggcagac acgacgcttg tgcgaccaag
      181 atcctgcttg atagagagtt tctgagtagg cggagtgggg caagatggtt aagtcctcat
      241 cctgtgggca agggagggaa aggtctccaa gacggctcag aacagtattt ttcttgagtt
      301 ttatgaggta gtattgccct gtttt
//
LOCUS       DAVHIU010000271          323 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVHIU010000271 DAVHIU010000000
VERSION     DAVHIU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.denovo.273"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..>323
                     /locus_tag="VVN06_002124"
     CDS             <1..>323
                     /locus_tag="VVN06_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5563585.1"
                     /translation="LFDQVASLLEEREIVTPTYREALITREKSFPTGLDMEFLGKDLP
                     NVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNIL
                     AQLMD"
BASE COUNT          101 a           59 c           64 g           99 t
ORIGIN      
        1 ctctttgatc aggtagcaag cttgttggaa gaacgagaaa ttgtcactcc aacttatcgt
       61 gaagccttga tcacgcgtga aaagtcattt ccaactggtt tagatatgga atttctagga
      121 aaggacttgc caaatgtagc gattcctcac acagatattg ttcataatct agctgagaaa
      181 gtggtggttg ttcgattaga aaaaccagta acttttcaca atatgatagc tcctgataag
      241 gaagtagaag tatctctact cttctttatc attaacaatt caagttcaag tcaaacgaac
      301 attctggctc agttgatgga ctt
//
LOCUS       DAVHIU010000272        24389 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.guided.020, whole genome shotgun sequence.
ACCESSION   DAVHIU010000272 DAVHIU010000000
VERSION     DAVHIU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.guided.020"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            270..584
                     /locus_tag="VVN06_002125"
     CDS             270..584
                     /locus_tag="VVN06_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5563586.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            600..986
                     /locus_tag="VVN06_002126"
     CDS             600..986
                     /locus_tag="VVN06_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU5563587.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1015..2400
                     /locus_tag="VVN06_002127"
     CDS             1015..2400
                     /locus_tag="VVN06_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5563588.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVQAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2403..2555
                     /locus_tag="VVN06_002128"
     CDS             2403..2555
                     /locus_tag="VVN06_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5563589.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2578..3840
                     /locus_tag="VVN06_002129"
     CDS             2578..3840
                     /locus_tag="VVN06_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU5563590.1"
                     /translation="MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLFEALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKRYNGR"
     gene            3785..3997
                     /locus_tag="VVN06_002130"
     CDS             3785..3997
                     /locus_tag="VVN06_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEU5563591.1"
                     /translation="MCQPYQLTDIQKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKE
                     VMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            4046..4129
                     /locus_tag="VVN06_002131"
     CDS             4046..4129
                     /locus_tag="VVN06_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEU5563592.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            4254..4991
                     /gene="erm(B)"
                     /locus_tag="VVN06_002132"
     CDS             4254..4991
                     /gene="erm(B)"
                     /locus_tag="VVN06_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU5563593.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(5248..6495)
                     /locus_tag="VVN06_002133"
     CDS             complement(5248..6495)
                     /locus_tag="VVN06_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5563594.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            6675..6896
                     /locus_tag="VVN06_002134"
     CDS             6675..6896
                     /locus_tag="VVN06_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563595.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            7013..7510
                     /locus_tag="VVN06_002135"
     CDS             7013..7510
                     /locus_tag="VVN06_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU5563596.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            7485..7991
                     /locus_tag="VVN06_002136"
     CDS             7485..7991
                     /locus_tag="VVN06_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5563597.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7975..10422
                     /locus_tag="VVN06_002137"
     CDS             7975..10422
                     /locus_tag="VVN06_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5563598.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            10425..12602
                     /locus_tag="VVN06_002138"
     CDS             10425..12602
                     /locus_tag="VVN06_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5563599.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKAERASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            12599..13600
                     /locus_tag="VVN06_002139"
     CDS             12599..13600
                     /locus_tag="VVN06_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU5563600.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            13597..14529
                     /locus_tag="VVN06_002140"
     CDS             13597..14529
                     /locus_tag="VVN06_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU5563601.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14774..14890
                     /locus_tag="VVN06_002141"
     CDS             14774..14890
                     /locus_tag="VVN06_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU5563602.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14906..16825
                     /gene="tet(M)"
                     /locus_tag="VVN06_002142"
     CDS             14906..16825
                     /gene="tet(M)"
                     /locus_tag="VVN06_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU5563603.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            17189..17434
                     /locus_tag="VVN06_002143"
     CDS             17189..17434
                     /locus_tag="VVN06_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563604.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            17431..17766
                     /locus_tag="VVN06_002144"
     CDS             17431..17766
                     /locus_tag="VVN06_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU5563605.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            17779..18147
                     /locus_tag="VVN06_002145"
     CDS             17779..18147
                     /locus_tag="VVN06_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563606.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            18134..18433
                     /locus_tag="VVN06_002146"
     CDS             18134..18433
                     /locus_tag="VVN06_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563607.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(18551..20014)
                     /gene="msr(D)"
                     /locus_tag="VVN06_002147"
     CDS             complement(18551..20014)
                     /gene="msr(D)"
                     /locus_tag="VVN06_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU5563608.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(20134..21351)
                     /gene="mef(A)"
                     /locus_tag="VVN06_002148"
     CDS             complement(20134..21351)
                     /gene="mef(A)"
                     /locus_tag="VVN06_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU5563609.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            complement(21737..21907)
                     /locus_tag="VVN06_002149"
     CDS             complement(21737..21907)
                     /locus_tag="VVN06_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU5563610.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            22429..22620
                     /locus_tag="VVN06_002150"
     CDS             22429..22620
                     /locus_tag="VVN06_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU5563611.1"
                     /translation="MTSKLTASTCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIE
                     IKQFKVTVITEPDAKTQSR"
     gene            complement(22680..23033)
                     /locus_tag="VVN06_002151"
     CDS             complement(22680..23033)
                     /locus_tag="VVN06_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5563612.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            23538..23960
                     /locus_tag="VVN06_002152"
     CDS             23538..23960
                     /locus_tag="VVN06_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5563613.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            23957..24187
                     /locus_tag="VVN06_002153"
     CDS             23957..24187
                     /locus_tag="VVN06_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5563614.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
BASE COUNT         7888 a         4510 c         4753 g         7238 t
ORIGIN      
        1 ccactcaatt tactactaat ttactactta tgaatgagct ttgatacgac gatttatcct
       61 tgaaaagtga agatataaag atacttccaa taaaatttga atatttaata ggtagacact
      121 tcaaaaaatg aggtgtctat ttttttaccc gattttgaaa ggaagtgaac ttatgaaaac
      181 aaaaaatcaa gaatcaaaag gtcgttcccc actctttaag accatcaaac attcattcag
      241 ccaataaaaa agaaaggata ggtaaaaata tggaacttaa atttgtgatt cccaacatgg
      301 aaaaaacatt cggcaattta gaatttgctg gcgaggataa agtcgttcag cgaagaatca
      361 acggacggct aactgtctta tcaagaagct ataatctcta ttctgatgtt caaagagcag
      421 atgatattgt ggtggtgctt cctgctgaag ctggcgaaaa acatttcggc tttgaggaac
      481 gtgtgaagtt agtcaatcca cgtattaccg cagagggcta caaaatcggc actcgtggtt
      541 ttacaaatta ccttttacat gctgacgaca tgataaaaga ataaagaaag agaggaaaaa
      601 tgatgagatt agcaaatggc attgtattag ataaagacac gacttttgga gaattgaaat
      661 tctctgctct acgtcgtgaa gtgagaatcc aaaatgaaga cgggtcggtt tcagatgaaa
      721 tcaaggaacg tacctatgac ttaaaatcca aaggacaagg acgcatgatt caagtaagta
      781 ttcctgccag cgtgcctttg aaagagtttg attataacgc acgggtggaa cttatcaatc
      841 ccattgcgga caccgttgct actgccacct atcaaggagc agatgttgac tggtatatca
      901 aggcagacga tattgtgctg acaaaggatt ctagttcatt caaagctcaa ccacaagcaa
      961 agaaagaacc gacacaagac aaatagtcgc taggtagaaa ggagactttt tcgcatgaaa
     1021 cagcgtggta aaaggattcg cccatctggt aaagatttag tctttcattt tacgatagcg
     1081 tcactcctgc ctgttttcct gctggttgtc ggactgtttc atgtgaagac aatccagcag
     1141 atcaactggc aggattttaa cctatcacaa gcagataaga ttgacattcc ctatttaatt
     1201 atcagtttca gtgtcgcaat tcttatctgc ttgctggtag cgtttgtatt caaacgggtt
     1261 cgctatgata cggttaaaca actttaccac cgtcaaaaac tggcaaagat gatacttgaa
     1321 aacaagtggt atgaatctga acaggtcaaa acagagggtt tctttaaaga tagtgctggt
     1381 cgtacaaagg aaaagataac ctacttccct aaaatgtatt atcgacttaa aaatggcttg
     1441 atacagatac gggtggaaat cacgctggga aaatatcaag accaactctt acacttggaa
     1501 aagaaattag agagtggctt gtactgtgag ctgacggata aagagttaaa ggattcctat
     1561 gtggaatata ctttgctcta tgacaccata gccagtcgta tttctattga tgaagtacaa
     1621 gctaaagatg gtaaacttcg cttaatgaaa aacgtatggt gggaatatga taagctccct
     1681 catatgttga ttgctggtgg tacaggtggc ggtaaaactt actttatact gacactgatt
     1741 gaagccttgc ttcatacaga ttcaaaactg tatattcttg acccgaaaaa tgctgacctt
     1801 gcggacttag gttctgtgat ggcaaatgtc tactatagaa aagaagactt gctttcttgc
     1861 attgaaacat tctatgaaga aatgatgaaa cgtagtgagg aaatgaagca gatgaagaac
     1921 tataagactg gcaaaaatta tgcttactta ggtctcccgg cacacttctt aatctttgat
     1981 gaatacgtcg ctttcatgga aatgctggga acaaaagaaa acaccgcagt tatgaataag
     2041 ctgaaacaga ttgtcatgtt aggtcgtcaa gctggcttct ttctaatact ggcttgtcaa
     2101 cgtccagacg caaaatattt aggcgacgga atccgtgatc agtttaattt cagagtggct
     2161 ttaggtcgta tgtctgaaat gggctatggc atgatgtttg gcagtgacgt acaaaaggat
     2221 ttcttcttaa agcgaatcaa aggtcgtggc tatgttgatg taggaacaag tgtcatatca
     2281 gagttttata ctccccttgt accaaaagga tatgatttct tggaggaaat taaaaagtta
     2341 tccaacagca gacagtccac gcaggcgacg tgcgaagcgg aagtcgcagg tgtggactga
     2401 tcttgctggc tggtgtggca atagccacgc cagcacttaa ccccccgtat ctaacagggg
     2461 ggtacaaatc gacaggaaac agtcaaaaaa acattagaaa atcctttggt tacaagggat
     2521 ttacaaaatt tcagcgtatg tcaaatgggc tttaaaagtt gacatacgcc tttttgattg
     2581 gagggatttt tactgaatga acaaacttgg ttacagcagt taaaagaaaa acgcttggct
     2641 tatggactat ctcaaaatcg tttagcggtt gcgactggta ttacaaggca atatctaagc
     2701 gatattgaaa caggaaaagt caagccatca gaggatttac agcaatctct ttttgaagct
     2761 ctggaacgct tcaatcccga cgctcccctt gaaatgctct ttgattatgt aaggattcgc
     2821 tttcccacaa cggacgtaca gcatgtggtc gaaaacatct tacaactgaa actgtcctat
     2881 tttcttcatg aggactatgg tttctattct tattcagagc attatgcttt aggcgatata
     2941 ttcgttctct gctcccacga actggacaaa ggagttctgg tggaattgaa aggtcgtggg
     3001 tgtcggcaat ttgaaagcta tcttctggct caacaaagaa gctggtatga gttctttatg
     3061 gacgctttgg tggctggcgg tgtgatgaaa cgccttgacc ttgccattaa cgataagaca
     3121 gggattttga atatccctgt actcactgaa aagtgccaac aggaagaatg tatctccgtc
     3181 ttccgcagtt ttaaaagcta tcgcagtggc gaactggtac gcaaagagga aaaggaatgt
     3241 atgggaaaca ccctctatat cggttcatta caaagtgaag tttatttctg tatctatgaa
     3301 aaggactacg agcagtacaa gaaaaatgat attcccattg aagacgcaga agtaaaaaac
     3361 cgttttgaga ttcgattgaa aaatgagcgt gcctattatg cagtccgtga tttactcgtc
     3421 tatgacaatc cagagcatac cgcctttaaa attatcaatc ggtatatccg ttttgtagat
     3481 aaagacgatt ccaaacctcg ttctgattgg aaactgaatg aagaatgggc ttggtttatt
     3541 gggaacaatc gtgaacgatt aaaactaacc acaaaaccag agccttactc cttccaaagg
     3601 acgctgaact ggctatctca tcaagttgcc ccgaccttaa aggttgcgat taaacttgat
     3661 gaaatcaacc agacgcaggt tgtaaaagac attctcgacc atgcgaaact gacagaccga
     3721 cacaagcaga ttttgaagca acagtcagta aaagaacagg acgtgataac aacaaaaaaa
     3781 ggatatctgt caaccatacc agttgacaga tatccaaaaa aaagatataa tgggagataa
     3841 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg
     3901 cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat
     3961 agtgcattat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
     4021 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgtaatg tagataaaac
     4081 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
     4141 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
     4201 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagagat tacatgaaca
     4261 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     4321 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     4381 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     4441 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     4501 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg aatattcctt
     4561 accatttaag cacacaaatt attaaaaaag tggtttttga aagccgtgcg tctgacatct
     4621 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     4681 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     4741 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     4801 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     4861 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     4921 taagtaccat tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     4981 ggaggaaata attctatgag tcgctttttt aaatttggaa agttacacgt tactaaaggg
     5041 aatggagata aattattaga tatactactg acagcttcca agaagctaaa gaggtcccta
     5101 gcgcctacgg ggaatttgta tcgataaggg gtacaaattc ccactaagcg ctcgggaccc
     5161 cttgtaggaa aatgtcctaa gtgggatatc tgtcaactgg tatggttgac taaaaatact
     5221 tcctacgaaa atgtaggggg tattttttta cgaaaaaata caatcgattc ttaaaaagaa
     5281 aaatttttga ttggcaaaac cataacaagt tcgttttagg gttttgattt tgcgattgat
     5341 gccttctaaa ggaccattag agtattcaaa tttagcgcta tttaagacat attttctgtt
     5401 ttgacgaagg gtttgaatag cagtatccat ttctgtattg gttttttggt agtctaagat
     5461 ggttgactct agtaattcac tattgcgctc gtttagggct ttcgtgatat cttggtaagt
     5521 ttggtatact tcagcgaact tggaaaattt actagtaatg agatcaacag cattttggcg
     5581 agtcatatat tgtttaacgc cgcgaagaaa aactacttct tcagggtgaa gatcttcagc
     5641 ttttttatgg aatagcttcc aatgtgactt cataatttta tattcttggc tctgtttatc
     5701 aagttgcttt aggatagaga tacgacaatt gtccaaagcg cgaccagcta attgtacaag
     5761 gtggaagcga tcaataatga tattggcatt agggaaaagg cgatagataa aactttgata
     5821 ttgagcattt aaatcaatta caactgattg aacgcattcg cgttcggctt ttgaataacg
     5881 actttcaaaa taatcaacaa tggtaggtga tagacgatcc tgtaactttg tgacaatttg
     5941 gtgggtttca gcgtcacaac agataaagga catcacagac ttaattgaac gaaactcgtc
     6001 aaaacataga tgcttaggca acttagccac acgatagtgt ggttccatgc gctctaagat
     6061 tgttcgacga acactgctag gagagcagtg acacatttca gcaataagct gaccagataa
     6121 gcctttacga gctaaaagca tgatttgatt tttgagatca ctggataagg tttgattttc
     6181 tttggttaaa ttagtaatag caccaaaagt agtatggcat gatttacatt tatagcgttg
     6241 tttacgaagc tctagttcat atcttctccc atttaaactt gccagtcgta catgagtttt
     6301 gcgaaagcca tccttattaa ctgtgggaaa gccacagtta cgacaacgat taatcggata
     6361 agaaagagta gctgttatta gcgttatata ctctttaaca gaatcgttgt tgtgttcagc
     6421 ttcttcaaca gaaataattt taatattttt atctttaatt ccaagaatat ttaggataga
     6481 atcattatgg gacatttgtt taaccttctt tcatgatttt tgtggtgaat tgattgtata
     6541 acgaggggac agcaaatgtc ctcttttttg tataaaaaaa tctggcatgg aatctctatc
     6601 cataccagaa agtgtatacc ccaaaaaaat aactcaaata caaattcatt gaatatagag
     6661 aggagaacat ttttatgaat tttggacaaa acctttataa ctggtttcta tcaaacgctc
     6721 aatcactggt gcttttagca atcgttgtga ttggcttgta tcttggcttc aagcgtgagt
     6781 ttagcaaact gattggcttt ttaattattg cgattattgc ggttggctta gtcttcaacg
     6841 ctgctggagt aaaagacatt ttactagagc tattcaatcg cattattggt gcttaaataa
     6901 aaccgttctt ttgtggaata taagtggttt tcttatgttc cgcaaaggaa tggtacacca
     6961 aacgaagtgc ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat atatggacga
     7021 tatgcaagtc tatattgcga atttaggcaa atacaatgag ggcgaattgg tcggtgcgtg
     7081 gtttaccttt cccattgact ttgaggaagt caaagagaaa atcggcttga atgatgaata
     7141 tgaggaatac gccattcatg actacgagtt accctttacg gttgacgaat acacttccat
     7201 tggcgaactc aatcgactat gggaaatggt atcggaatta cccgaagaat tacaatcgga
     7261 gctatctgct ctgctcactc atttttcaag cattgaagaa ctaagcgaac atcaagagga
     7321 tattatcatt cattccgatt gtgatgatat gtatgacgtg gcacgctact acattgaaga
     7381 aacgggtgct ttaggcgaag taccagctag tcttcaaaac tatattgatt atcaagccta
     7441 tggtcgggat ttagaccttt caggaacgtt tatctcaacc aatcatggga tttttgaaat
     7501 cgtctattaa atctgtcggt acattactac tggcagattt tctattttac ggggtggctc
     7561 aatcagctac ccctattttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     7621 ccagtatctg gtctgtggaa aaggtactgt attctatcaa tgattttaga cttccgtttc
     7681 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtg atgatacttg
     7741 gcaacttgcc ccctctttcc atgatagagg gagcatttct caaatacttt gggattcctg
     7801 tggctttcac atggtttatg tctacaaaaa cttttgatgg taaaaagcct tatggatttt
     7861 tgaagtctgt cattgcttat gcactgcgac caaagctgac ctatgcagga aaaaaagtaa
     7921 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     7981 tatccaatta aatacattga aaacaatctc gtctggaata aagacgggga atgttatgct
     8041 tactatgagc ttgttcctta caattactca tttctaagtc cagaacagaa aatacaagtg
     8101 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaaaattca tgctttacaa
     8161 atcagtacag aatccagcat acgttctgca caagagcgtt ccaaaaatga agtcactggc
     8221 aagctcaaag cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     8281 attggcgaaa atcaagtgaa ctaccgtttc tttatcggct ttaagttgct tctcaacgat
     8341 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgctttgtc tgattttgtc
     8401 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     8461 cgttttcaga agatggaaaa gctcttagaa aataaaatct ctcgtcgttt caaaatccgc
     8521 aggttagata aggacgactt cggctatctg attgaacacc tttacggaca gacaggcact
     8581 gcctatgaag agtatgagta ccatctatca aagaaaaagc tggataatga aacgctgatt
     8641 aaatactatg acttgattaa gcctactcgc tgtttggtgg aagaaaaaca gcgatatttg
     8701 aaaatccagc aggaagatga aaccgtctat gtagcttact ttaccattaa cagcattgtc
     8761 ggagaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttacattc
     8821 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     8881 cgcaataaaa agaaagaact gaaagacttg gataaccacg catggcaaag tgataatgaa
     8941 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     9001 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     9061 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     9121 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     9181 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     9241 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     9301 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     9361 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     9421 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     9481 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcact
     9541 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtgattat gaaaaatccc
     9601 aaagattctg aatcactggc tattgatatt ctgacattcc ttacggggat ttcctctcgt
     9661 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagta
     9721 cgagggttga tgaaagtgat tgaggaatta cgggttgaga atacgccact aagtaccagt
     9781 atagccgacc atatcgaaag ttttacagac tatgactttg cacatttatt attcagtaat
     9841 ggttatgtgg agcagtctat cagcttagaa aaacaactga acattataca ggttgcggac
     9901 ttggtacttc ccgacaagga aacttccttt gaggaatata ccactatgga gcttttatcc
     9961 gttgctatgc tgattgtcat tagtaccttt gctttagact ttatccatac agaccgaagc
    10021 attttcaaga ttgtagattt agacgaagca tggagctttt tacaggtagc acaaggaaaa
    10081 acactatcta tgaagctggt tcgggctggt cgtgctatga acgctggggt atatttcgtg
    10141 acccaaaata cagacgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
    10201 gcatttcgtt ccactgacct taacgagatt aaaaagacct tagccttttt tggtgtagac
    10261 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
    10321 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
    10381 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatag
    10441 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
    10501 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
    10561 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
    10621 actatcaact ggatttttat gtggataata gctggggctg gcttccgtgg aactggtcgg
    10681 acgggattgg aaaacaggtc atgtatggac tatatgccat taccaatttt atttggacaa
    10741 tcagtttgta tgtttccaat gcgacaggtt acttagtaca ggaagcctat tccttagact
    10801 tcatttccgc tacagcagat tccattggta agaatatgca gaccttagct ggtgtgagtg
    10861 caaacggatt ttcaacagag ggtttctatg ttggattcct cttactcttg attttggttc
    10921 ttggggttta tgttgcctat acgggactga taaagagaga aaccacaaag gcaattcatg
    10981 ccattatgaa ttttgtgctg gtgtttatcc tatcggcttc ctttattgcc tacgctcccg
    11041 actacattaa aaaaatcaat gacttttcat cagacatcag taatgccagt ttatcacttg
    11101 gcacgaagat tgtcatgccc cattccgata gtcaaggcaa ggacagcgtg gacttaatca
    11161 gagatagcct gttttccata caggttcagc aaccgtggct actgcttcaa tacaacagtt
    11221 cagacattga aagtatcggt attgaccgtg tggaaagcct gctctccacc agcccagatt
    11281 ccaacaatgg cgaagacaga gaaaaaattg ttgcggaaga aattgaagac agaagcaata
    11341 ccaatctaac cattacaaag accattaacc gtttaggtac agtcttcttc ctatttgtct
    11401 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
    11461 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
    11521 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
    11581 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
    11641 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
    11701 acttcaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
    11761 tgggaagtcg tgtgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
    11821 agcggaaact tggacgttcc atgactactc taggggctgg gtctgccatt gttacaggta
    11881 aaaaaggaca gtcgggttcg gggagttctg caaggacaca agcagatcac tcccgaccag
    11941 acggaaagga aaaatcaaca cttggaaaac gtatcggtca aaccatcggt acagtagctg
    12001 ataccaaaga cagaatggta gacactgcta gtggtttgaa agaacaggtt aaagatttgc
    12061 cgaccaatgc aagatatgca gtatatcaag gaaaatccaa agtaaaagag aatgtccgtg
    12121 atttaaccag tagtatttct caaaccaaag cggaaagagc cagtggacgc aaggaacagc
    12181 aggaacaaag gcgaaaaacc attgcgaagc gtcgctctga aatggaacag gtcaaacaga
    12241 aaaaacagcc tgcttcttct gttcatgaaa gaccgactac aagacaagaa caatatcatg
    12301 atgaacagac ctcaaaacag tctaatattc agacttcata taaggaatct caacaagcca
    12361 aacaagagcg tccagcagtt aagtccgatt tttcaagtcc aaaagtggaa cgccaaggca
    12421 ataccgttca agaaaaaacc gttcaaaagc cagcaacttc aaccactaca gcagatagaa
    12481 cttcacaacg tccaatcaca aaagaacgtc cgtctactgt tcaaagagta ccactacaaa
    12541 atacaagaag tagaccacca atcaaaaccg ccaccattaa gaaagtcggt aagaaaccat
    12601 gaagttgaaa actttagtga ttggtggttc tggattattc ttgatggtct tctcactgct
    12661 tctgtttgtt gccattttat tttcagatga acaggacagc ggaatttcca atattcatta
    12721 tggaggtgtg aatgtttccg cagaagtgct ggctcataag cctatggtag aaaaatatgc
    12781 caaagaatat ggcgttgaag aatatgtcaa catacttctt gcgattatac aggtggaatc
    12841 gggcggtact gcggaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
    12901 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgaat tattagccag
    12961 tagcgaaagg ctcagtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
    13021 tttcttaggg tatgtggcta atcgtggaaa taaatatacc tttgaactgg ctcaaagttt
    13081 ctcaaaagag tattcaggtg gcgaaaaagt gtcttacccc aatcccatag ccatacctat
    13141 caatgggggc tggcgataca actatggcaa tatgttttat gtgcaactgg taacgcagta
    13201 tcttgtcaca acagagtttg atgatgatac ggtacaagcc atcatggacg aagcactgaa
    13261 atatgagggc tggcgatacg tttacggtgg agcttccccg actacttctt ttgattgtag
    13321 cggactgaca caatggacgt atggaaaagc tggaattaac ttaccacgaa ccgcacaaca
    13381 gcaatatgat gtgacccagc atatcccact atcggaagca caagctggcg atttggtttt
    13441 ctttcattct acctataacg ctggctctta tattactcat gttgggatat accttggcaa
    13501 taaccgtatg tttcatgcag gcgacccaat cggttatgcc gacttaacaa gcccctactg
    13561 gcaacagcat ttagtgggag caggacgaat caaacaatga gaaaggaaga tttaatgatg
    13621 aaatttagaa aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa acctcgtgtc
    13681 tataaggtca atcctcataa aaaggttgtg attgccttgt gggtactttt agggcttagt
    13741 ttcagctttg cgatattcaa gcactttaca gctatagata ctcatactat tcacgaaaca
    13801 actatcatag aaaaggaata cgttgatact catcatgtag aaaattttgt agagaacttt
    13861 gcgaaagtct actattcatg ggagcaatcc gataagtcca ttgataatcg aatggaaagt
    13921 ctaaaaggct atctgacaga tgaacttcaa gctctcaatg ttgatacagt acgcaaagat
    13981 attcctgtat cgtcttctgt aagaggattt cagatatgga cggtagagcc aactggcgac
    14041 aatgagttta atgtaaccta cagtgtagac cagctcatta cagagggaga aaatacaaag
    14101 accgtccact ctgcttatat agtgagtgtc tatgtagatg gttctggaaa tatggtactg
    14161 gttaagaatc cgaccattac caacatacct aagaaatcaa gttataaacc aaaagccatt
    14221 gaaagtgagg ggacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
    14281 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
    14341 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tattcacaat
    14401 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
    14461 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
    14521 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
    14581 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
    14641 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
    14701 cgaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
    14761 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
    14821 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
    14881 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
    14941 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
    15001 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
    15061 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
    15121 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
    15181 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
    15241 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
    15301 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
    15361 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
    15421 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
    15481 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc ataattgttc
    15541 cctgttccct gtttatcacg gaagtgcaaa aaacaatata gggattgata accttataga
    15601 agtgattacg aataaatttt attcatcaac acatcgaggt cagtctgaac tttgcggaaa
    15661 agttttcaaa attgagtatt cggaaaaaag acagcgtctt gcatatatac gtctttatag
    15721 tggcgtactg catttgcgag attcggttag aatatcggaa aaggaaaaaa taaaaattac
    15781 agaaatgtat acttcaataa atggtgaatt atgtaaaatc gataaggctt attccgggga
    15841 aattgttatt ttgcagaatg agtttttgaa gttaaatagt gttcttggag atacaaagct
    15901 attgccacag agagagagaa ttgaaaatcc cctccctctg ctgcaaacga ctgttgaacc
    15961 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
    16021 cccgcttctg cgatattatg tggattctgc gacacatgaa atcatacttt ctttcttagg
    16081 gaaagtacaa atggaagtga cttgtgctct gctgcaagaa aagtatcatg tggagataga
    16141 aataaaagag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
    16201 tcacatcgaa gttccaccga atcctttctg ggcttccatt ggtttatctg tatcaccgct
    16261 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
    16321 atttcaaaat gcagttatgg aagggatacg ctatggctgt gaacaaggat tgtatggttg
    16381 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
    16441 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
    16501 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
    16561 acgagcatac accgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
    16621 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
    16681 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
    16741 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
    16801 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
    16861 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggagatag
    16921 ttattataaa ctacaagtgg atattgtgtg ctgagagctt tcttctatac taatagacga
    16981 aagggtgtga aaatgatttt taaatgatac tgtggaacgg aacagtagcc ctagtattga
    17041 ctactgtcgt ttctattcat attggctatt ctaggactga gatgaaaaaa tctataaatg
    17101 ctcagaataa aattgaaccc gcaaatctcc ccaaaacaat ggtgagtcat gtacttgtat
    17161 tattccgaaa aaatacacct ctggtgcagt gagacaaatt ggtgtatctt atagtggctt
    17221 cgtagatgaa agctatactc tactatcact ctttgatgat gtagaacaaa ttgaaaaaga
    17281 taatagactt cagacagcta ttgatgttgt cagagaacag tttggttttt tagccataca
    17341 aaaaggaacc gtcctaactg aaggttccag aaatattgaa cgcagtaaac ttatcggtgg
    17401 tcattccgcg ggtggattgg agggattaaa atgaaacaag aaaaaaatac agtacaattt
    17461 tcagaaatcc gtagcaaagg atgtaatgat attgaaatgc ttgaaagatt tttacatgga
    17521 atcgttgaaa cagcaacttc aaaacttcgt cagagaaaac tcaaaacaac tgaaatatcg
    17581 atacgactag tacatgctaa atctgaaaac cgattaccat tggaatttac atttagcatt
    17641 aagccaacaa gctcatctgt gataatctat actgaggtaa tcaatcgctt taaagaatgt
    17701 tacacaggtg ggggaattca aggttttacg attcaatttg ataaaaatac ccttgcctct
    17761 gcatagaaag gatttgatat gattgaccgt tcatatttac catttcaatc agcaagagag
    17821 taccaggata caaagatgca aaaatggatg ggctttttcc tatctgaaca tgcatcagca
    17881 ctctctgatg atacaaacaa agtaacgtac atgtctgact tatcactaga gaagaaatta
    17941 ttactcctca gtcaagtata cgccgggcag ctacgcacac gcattcaagt gattgaaaaa
    18001 aacaagcgtg tttcctacac tggaacaata ccaagtctga ccaaagattt cattttgata
    18061 aaaactacaa caggtcacat caatttgaaa ttaaaagaca ttattagtat tgaacttgtc
    18121 gaggaggtgc tctatgaatc agcttgagtt tcagcgtaat cacctacaaa tggactatta
    18181 tagcgagagc taccaagatt ttgaacgtga cttctaccgc tactctaaca tgaatattcc
    18241 attgaccttc ctaactgatg atatcctaaa aacaatggcg acttcacgta agaattactt
    18301 tgtcctcaat aaggaaaagt ccagagataa ccgcgatcac ttcttcatat ttgaagtaag
    18361 taccgtagat gagaatccgc taatctatca ttatacatat aagaaaacta caatatattt
    18421 agcagaaaaa taggagcagt tcaattgact gttcctattt ttaatattca taaaatctaa
    18481 agtctttata ctctttaaca atggagtcgc caaccagaac agactatact gaccagcgac
    18541 taccttaaat ttaatgtttc agatttattt tcttatctct aatttcataa actacatctg
    18601 ctacattttc gagtaatcgt ttatcgtggg tgataaacac gatagttccg gtgtactcct
    18661 tcattagtat ttccaaagcc tctaaacttg gtatgtcaag gaagttactg ggttcatcca
    18721 ttattaggat gttatatcta cccatgagca ttttagcaag caacaatttt ataatttctc
    18781 caccgcttaa aacagataaa ctttttccaa tatcgttctg tttgaacccc atagatgcta
    18841 gcactgaacg aatttctgat atattgtagt cacaatcctt ctgcataaac tccataacat
    18901 tctgattact gttgtacttg taaccattct gtgcaaagta acctattttt gccttaggcg
    18961 aaatagaaat tccttcttca tggtttaaga tcatttggat taaagttgtt tttccgattc
    19021 cattaccacc agttaacgcc acttttgctc ctaacggaat ttgaaaagat gcattttcaa
    19081 acagagcctt atccccaaat actttattaa tttctgcacc gactataggg tatggattat
    19141 ggagctccaa tgctttactt tgcctgaaac gaattctgcg aatgccttcc ggagcttcta
    19201 cttttcctaa ggccgcaatc ctgtgctcta gggttttagc agcattatac atcttttttt
    19261 ccttacttcc tattgatttt tgatgagcta aacgccctcc gtcttcagta ctttttttct
    19321 ttgaagaacc ttttgccttc tgttctattt tacgagcctg ttttcgcttt tcctccgcag
    19381 ccctttccaa tcgggcacgt tccgcaataa attgttcgta ttctgcagct tggctcttac
    19441 gttcttcctc tttctgacga agataatcag aatagtttcc ccaatactca gtgattttgc
    19501 catctttcag ttcccatatt ttatctacta tttcatcaag aaaatagcgg tcatggctaa
    19561 taactaacag tgcacctgta aaatatttta gctgtcctat tagaaaatca attccttcac
    19621 ggtctaaatg gctcgtaggt tcatccgcta aaataccatg aacctgtgcc gataaggcct
    19681 gtgctatttt aagccttgtt tcttcaccac cgctcatagt ctgtatattt aattgctcaa
    19741 cacctagctt gcctacaagt gcaaaatctt tttcctcctg cagagttact tcgtccaact
    19801 ggggaatata ggcaagttca cccagacgat tcattttaca tcctggggga gttaattctc
    19861 ctaaaagtac cctgagtaaa gtgctttttc cagcaccatt tgctcctact aaaccaatac
    19921 ggtcataatc atatacttct aattcattta tatctaaaac atcgcgtcct ttgaattcca
    19981 cacgaatgtc ttttgctttt aatattaatt ccataacatt tcctcctgtc tataatcgca
    20041 tgctttcatt tgcttgtatg cagggaaaac cctgcgattt tagcaggaag agttacatga
    20101 aaataagata cataaatatt cctccaatat tgtttatttt aaatctaatt ttctaacctc
    20161 agttatcatt tggcaaacta tagcaatgcc aataattaaa atacctgata gtaaaaacca
    20221 atgatttaca ccgattttat cagcaaagaa tccagaaaga attaacccaa ttggcatagc
    20281 aagtgacatg atacttccga tcaaagaaaa tacacgtcct aaatattcag gcttaatttt
    20341 ctcctgaaaa agagctgttt gcacaccgct ataaaatggc accgaaagcc ccattattgc
    20401 acagcaaact acgaatatta caaatccatt tggaggaagt attcccgaaa cggctaaact
    20461 ggtccccatt ataaaaaatg aacttgttat tagtaataca tgcttttcga agccccctaa
    20521 tcttcctaat aataagcctc ctgctagcat cccaaatgca aaggaaattt ccgtaataga
    20581 aatatgcaca ggcgttccat taaagtgttc catgcttatt aaaggaaata gtgcattgat
    20641 tggcatataa acaaaagtat atagtgttcc taagagtaat aaggcaaaca atcctttgtt
    20701 ttgtctcaga accacaactc cttctttcat ctcccttatg aaatttggtt ctaaactttg
    20761 cacttgatta cccagcttag gtatacgtac aattgctacc gtaatagatg caatcacagc
    20821 acccaatacg tcgatggcaa taatagcatt taaatcccaa acggagtata agagtgctgc
    20881 aactgccgga ctaacaatat agcttataga ctgcaaagac tgactatagc ctgcgcattt
    20941 cgttagctgt tcttctggta ctaaaagtgg tgtaaccgca ttgagtgctg gggtatgaaa
    21001 agctgttcca atgctacgga taaacaatac tatcataatc atccagacag gtagctccat
    21061 acagaatgca acaatagcaa gcactgcacc agctgctgcg ataattaaat cggcaccaat
    21121 cattatcttc ttcctatcat gacgatccac tagcacacca atggcaggtc ccaaaatcgc
    21181 atagggtaaa aaacctacta atgaagccat agacaagacc atcgcagatc ctgttttttc
    21241 tgtaaggtaa aaaataatcg ccatttgcag gatggcacta gtgattaatg atactgcttg
    21301 ccctgcccat attgcataaa attttcgttt ccaattgttg tatttttcca tttatattat
    21361 ctcctgcata ttattttgct tgaatttcta ttttgaatag cattctaggc aataaaaaat
    21421 gcaggccaaa ccccacaatg tggcttttgg tctgcataca tacaatttgg aaacattcat
    21481 attaaagaca tagttaaata aaggtatagt taaataacca atatcctcac cgtaactaat
    21541 gaatgctcaa tatcgtataa ataagcacaa caaaaaagcc tatcatcggg tatagattct
    21601 gcttttttta ttgccagctt atcttaaacg cattgaggct gtcatagttt cggttcctcc
    21661 tacatctttg tttatatcaa tttatagtat aacacaacaa gatgatatgt tcaatataaa
    21721 agttatggaa tgagactcat acttccaatt cgatgccaga tttaaaggat atgacgaagt
    21781 tttcttcata gactgtaacg ctctggatta tcttccttag tagcaagcga ttagctttca
    21841 caaaatcttc tgtttgtagt tttaaaaatt catcaggatt ttctaactca acctcaaaat
    21901 atttcatttt acattccctc atttcattta ttgataaatt gagtttgcaa aaaagagtgg
    21961 acaatttttg tctactctta acctttaaaa tagttttttt taatcgattt gaagttgcct
    22021 aaattattac ttattcggta aaatgaagta ttgctttcaa cagatttcct tcaactacac
    22081 ttcacttgat tcaaacaagg tgggtacatt tctattccca caaactcctt gtcaatggaa
    22141 acaaacacgt acccacaggg taaatggaaa tagaaactga taatttctag ctatcacttc
    22201 tactcattcc aaaaattttc tcactctgat acttacccac cataaagcaa aaagccttgc
    22261 aatcaaggct ttcattatcc ctttcgttca aaggtttcta agcttttacg agcagagcga
    22321 cacactcagc ggttcgctat ctccgttctg tctgcgtgct agcacttgtc aatcacggac
    22381 agctatcgca tgggcggaag taaatgctaa tcttcgtcgt tttactcctt gactagcaaa
    22441 cttaccgcct caacatgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat
    22501 actgaattaa aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa
    22561 ataaagcagt tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa
    22621 aatgagatta atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat
    22681 tagtcttcga tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt
    22741 tctttgtctg taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc
    22801 aagttattag caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca
    22861 taaagaactt gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg
    22921 tcaatttcaa tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct
    22981 ttaatggcta aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac
    23041 cacctctata cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat
    23101 atggtttata ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt
    23161 atatggtagt gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt
    23221 tggaggtcaa cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct
    23281 tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa
    23341 tcatatagat agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt
    23401 ttggctattc agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt
    23461 cacattggga ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca
    23521 gaaagggggt gagaaacatg aaaccatctt ctttccagac cacaatagaa aatcagtttg
    23581 actatatctg taaacgtgct atggaagacg agcgaaagaa ttatatgctt tatctttcaa
    23641 ggattgcaaa gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt agccagtttg
    23701 cgacaacaga taactattca actgactttc agatttttac actcaatggg ttatcagtag
    23761 gcgttgaaaa tgatttgttg agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa
    23821 ttctactgct gttttacttt atggacatga gcgattcaga aattgcagac ctgttgaaat
    23881 tgaaccgttc tactgtctat cggcatagaa ccagtggact agccttaatt aaaaagttta
    23941 tggaggaatt tgaagaatga aaacacaata tcctatgatt ccctttcctc tcattgtaaa
    24001 ggcaacagat ggcgataccg aagcgattaa ccagattcta catcattaca gagggtacat
    24061 aacgaagcgt tccctacgac ttatgaaaga tgaatatggc aatcaaagta tggtcgttga
    24121 tgaagtctta cgtggaagaa tggaaaccag actgattaca aagattttgt catttgaaat
    24181 taagtaatat cctctctcct ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca
    24241 gggaggtttg ttattccacc aaagcatatt gagctttcaa tgtgttttga taggctaacg
    24301 agccattgtt ctttgaaaac tgaataaaag taatcgaata cgtttcgata agaaaagagc
    24361 caacggaact aaccgccatg acctatctt
//
LOCUS       DAVHIU010000273        22187 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 82-144
            SAMN08166724-rid19298373.guided.023, whole genome shotgun sequence.
ACCESSION   DAVHIU010000273 DAVHIU010000000
VERSION     DAVHIU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08166724
            Sequence Read Archive: SRR6382474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001869599.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001869599.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/31/2023 15:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 46 of 119
            Pseudo Genes (internal stop)      :: 36 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08166724-rid19298373.guided.023"
                     /serotype="19 F"
                     /isolate="82-144"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Russia: Saint-Petersburg"
                     /collection_date="2011-03-29"
                     /note="genotype: ST 3971"
     gene            <1..668
                     /locus_tag="VVN06_002154"
     CDS             <1..668
                     /locus_tag="VVN06_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5563615.1"
                     /translation="AQVGAYLQGEEVILYQSFSEIKELISEDIVVDYIMETRALLKMM
                     GLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGKFIKPKAGSKVFTGRVVNGTH
                     DLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVRPYTGDYHAQFDASVIDEAIS
                     CWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLSPLKSINFHRARWKEMVKSYF
                     EKK"
     gene            760..1614
                     /locus_tag="VVN06_002155"
     CDS             760..1614
                     /locus_tag="VVN06_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5563616.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMCISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            1726..2610
                     /locus_tag="VVN06_002156"
     CDS             1726..2610
                     /locus_tag="VVN06_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU5563617.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            2773..4512
                     /locus_tag="VVN06_002157"
                     /pseudo
     CDS             2773..4512
                     /locus_tag="VVN06_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            4787..5089
                     /locus_tag="VVN06_002158"
     CDS             4787..5089
                     /locus_tag="VVN06_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563618.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            5145..5573
                     /locus_tag="VVN06_002159"
     CDS             5145..5573
                     /locus_tag="VVN06_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5563619.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
     gene            5631..6240
                     /locus_tag="VVN06_002160"
                     /pseudo
     CDS             5631..6240
                     /locus_tag="VVN06_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            6522..6769
                     /locus_tag="VVN06_002161"
                     /pseudo
     CDS             6522..6769
                     /locus_tag="VVN06_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            6874..7176
                     /locus_tag="VVN06_002162"
                     /pseudo
     CDS             6874..7176
                     /locus_tag="VVN06_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            7332..8561
                     /locus_tag="VVN06_002163"
     CDS             7332..8561
                     /locus_tag="VVN06_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5563620.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            8581..11277
                     /gene="ppc"
                     /locus_tag="VVN06_002164"
     CDS             8581..11277
                     /gene="ppc"
                     /locus_tag="VVN06_002164"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU5563621.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITHKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    11360..11577
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11690..12694
                     /locus_tag="VVN06_002165"
     CDS             11690..12694
                     /locus_tag="VVN06_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5563622.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            12763..13629
                     /locus_tag="VVN06_002166"
     CDS             12763..13629
                     /locus_tag="VVN06_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5563623.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            13626..14384
                     /locus_tag="VVN06_002167"
     CDS             13626..14384
                     /locus_tag="VVN06_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5563624.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            14474..16234
                     /gene="dnaG"
                     /locus_tag="VVN06_002168"
     CDS             14474..16234
                     /gene="dnaG"
                     /locus_tag="VVN06_002168"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5563625.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYQAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            16237..17346
                     /gene="rpoD"
                     /locus_tag="VVN06_002169"
     CDS             16237..17346
                     /gene="rpoD"
                     /locus_tag="VVN06_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU5563626.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            17360..17689
                     /locus_tag="VVN06_002170"
     CDS             17360..17689
                     /locus_tag="VVN06_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5563627.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            17804..18847
                     /gene="cpoA"
                     /locus_tag="VVN06_002171"
     CDS             17804..18847
                     /gene="cpoA"
                     /locus_tag="VVN06_002171"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU5563628.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            18863..20188
                     /locus_tag="VVN06_002172"
     CDS             18863..20188
                     /locus_tag="VVN06_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5563629.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            20219..20395
                     /locus_tag="VVN06_002173"
     CDS             20219..20395
                     /locus_tag="VVN06_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5563630.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            20454..20588
                     /locus_tag="VVN06_002174"
     CDS             20454..20588
                     /locus_tag="VVN06_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU5563631.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            20652..21962
                     /gene="obgE"
                     /locus_tag="VVN06_002175"
     CDS             20652..21962
                     /gene="obgE"
                     /locus_tag="VVN06_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5563632.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            21972..22130
                     /locus_tag="VVN06_002176"
     CDS             21972..22130
                     /locus_tag="VVN06_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5563633.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         7013 a         3673 c         4885 g         6616 t
ORIGIN      
        1 atgctcaagt aggtgcctac ttacaaggag aagaagttat tttatatcaa tctttttctg
       61 aaataaaaga gttaataagt gaagatatag ttgtagatta tataatggag actagagcat
      121 tactaaaaat gatgggctta aacgttccgg tttatgacta tcctattgag cttaaagagt
      181 tttatggtcg aaagatttat gctggtattt taggagagat tgtgaatata cctgataatt
      241 ggggaaaatt tattaagcct aaagctggtt caaaagtctt tactggaaga gttgttaatg
      301 gaacccatga tttaataggt attggtctac ctttcgacta tcctatatgg attagtgagg
      361 ttgtagaatt catagctgaa tggcgttgtt ttgtgttaga tggtcgcgta ttagatgttc
      421 gaccctatac aggtgattat catgcacaat ttgatgcaag tgtaattgat gaagcgatat
      481 catgttggaa agatgcgcca atagcttatg gactagatat cggtgttact cgcgatggca
      541 gaacacttgt tgttgaagta aatgatggtt atgcattggg aaattatggc ctatctcctt
      601 taaaatcaat caattttcat agagctaggt ggaaagaaat ggtaaaatcc tattttgaaa
      661 aaaaatgaaa tttttaaaat ccaacaggat gttattttct aaaataaata tggtagataa
      721 ccaatataaa aagcatcaaa atgaacaagg aggaatctga tgcttggagc aattattgga
      781 gatattgtcg gttctgttta cgaatggaac aatatcaaaa caaaggactt tcctttattt
      841 cggaaggact gcttttttac agatgacacg gttatgacct gtgctgttgc agaagcaatt
      901 atgaatggtg gacaaaaaga cgatttcatt gacgcgatga agaaatatgg cagaatgtat
      961 ccgaatgctg attacggtgc tcggtttaat gcatggctaa acagcgataa ccgtgagcct
     1021 tataatagct ttgggaatgg atcagctatg tgtatttctc catgtgcttg gatcatggac
     1081 tgtggttttt atgcgaaaac tggtatgtgg ccatcatcta gaggacttac gagtctttct
     1141 gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg ctatggctac agctgatgct
     1201 atctttctat gtcgttttta ctttggtggt tattgtagag agtacgagca atcaattaac
     1261 gacaatccta cagagtgtaa aagacgaatt aaggattata tagaaaaaga atacgattac
     1321 aatctatctc aaactttaga tgaaatccgt cctaactatc gttttaacga aacatgtcag
     1381 gaaactgtcc ctcaagccat tatcgccttt cttgaaagta gagacttcga agatgcgata
     1441 agaaatgcca tctcacttgg tggcgacagt gatactctcg cagcaatcac ttgtaacata
     1501 gcagaggcag cttacggtat tcctgattgg atcaaggata aggcctattc ttacttggat
     1561 gaacccttaa aggatgtagt taggcgatgg gaaaatagaa taaaagcgta ttaatctcca
     1621 gtacgtcaga tttctctttt ctgtattttc attgtataat aggattacat ttacttgcat
     1681 acgacgaaat gtaaacattt ctgtttaaaa ttaaggataa aatcaatgaa aaaagcaatg
     1741 gtaattatca accctacttc tggtggcgag aaggctttgg attacaaaga aaagctggag
     1801 aataaagcaa aagaatactt tgaatatgtt gaaaccaaaa ttaccgaaaa agcgctggat
     1861 gcaacacatt ttgctgaaga agcttctcgt gagcagtatg atgcagtggt tgtgtttggt
     1921 ggagatggaa ctgtcaatga agtcatttca ggtattgatg agagagacta cattcctaag
     1981 ttagggatta tcccaggcgg tacgggtaac ctcattacaa aacttttgga aatcaatcaa
     2041 gacatcgatg gcgcaattga cgaactggat tttgatttaa ccaataagat tgatatcggt
     2101 aaagcaaatg acaactattt tggttatatc tttagtatcg gttctctgcc tgaggcgatt
     2161 cacaatgttg aaatagagga caaaacaaaa ttcggtattt taacctatgc tgtaaatacc
     2221 atgaagtctg tcatgacaga tcaggtcttt aacattaagg ttgagacaga aaatggaaat
     2281 tatgttggtg aagctagcca tgttttggtc cttttgacaa attacttcgc tgataagaaa
     2341 atctttgaag aaaacaagga cggctatgcc aacattttga ttctgaaaga tgcctctata
     2401 ttctccaaat tatccgtcat tcctgattta ttaaaagggg atgttatcgc aaatgataat
     2461 atcgagtata tcaaagcgcg taatattaaa atctcttcag atagtgaatt ggagtcagat
     2521 gttgacggag ataaatcaga taacctacct gtagaaatca aagtcctagc tcagcgagta
     2581 gaagtatttt caaaaccgaa agaggattag tatatagaga aagccttttt taaggctttt
     2641 tgtatacttt aaaagatagt tcctttaaca acggacattc cttgcaaata gtttacaaaa
     2701 atagtatact ggattcattg agtttgaaaa cgtttgcgta aaatttgaat gaatacttta
     2761 ggagacaaat tgatggaatt gagtgctatt taccataggc ctgagtcgga gtatgcctat
     2821 ctttataagg ataagaaact ccatattcga attcgaacta agaaagggga cattgaaagc
     2881 atcaacttgc actatgggga cccttttatc tttatggagg agttttatca ggatacaaaa
     2941 gaaatggtca agataacttc tggtacctta tttgaccatt ggcaggttga agtgtcagtt
     3001 gactttgcac gtatccagta tctctttgag ctcagagata cagaaggtca aaatattttg
     3061 tatggcgata aagggtgtgt ggaaaattct ctagaaaatc ttcatgcaat tgggaatgga
     3121 tttaagttgc cttatcttca tgagattgat gcctgcaagg ttcctgactg ggtttcaaat
     3181 acggtatggt atcagatatt tcctgaaaga tttgccaatg gcaatgctct attaaaccca
     3241 gaagggactt tagactggga ttcatctgtc acacctaaga gcgatgattt ctttggtggt
     3301 gatttacagg ggattattga tcatatggat tacttgcaag acttgggtat tactggacta
     3361 tatctttgtc ccatctttga atctacaagc aatcacaagt acaatacgac agattacttt
     3421 gaaattgacc gtcattttgg agacaaggag acctttcggg aactggtgga tcaagcgcat
     3481 catcgtggca tgaaagtcat gctggatgcg gtatttaatc atattggttc gcaatctctt
     3541 caatggaaaa atgtcgtcaa aaatggtgaa cagtctgctt ataaggattg gttccatatt
     3601 caacaattcc cagtgacaac tgaaaagcta gttaataaga gagacttacc ctatcatgtt
     3661 tttggtttcg aggactatat gcctaagcta aatacagcca atccagaggt caagaattat
     3721 cttttaaagg ttgcgactta ttggattgaa gagtttaata tcgatgcttg gcgtttggat
     3781 gtggctagtg agattgacca tcagttctgg aaggattttc gtaaggcagt tttagctaaa
     3841 aatcctgatc tttatatcct aggagaagtc tggcatacat ctcagccttg gctaaatgga
     3901 gatgagttcc atgccgtcat gaattatcct ttatctgata gtatcaagga ctatttctta
     3961 cgaggaatta agaagacaga ccagttcatc gatgaaatca attgagagtc tatgtattac
     4021 aagcagcaga tttcagaggt catgtttaat ctcttggatt cacatgatac agagcgaatc
     4081 ctgtggacgg ccaatgaaga tgttcaactg gttaaatcag ccttagcctt tctcttttaa
     4141 caaaaaggaa caccgtgcat ttattacgga accgagctag ccttgactgg aggaccagat
     4201 ccagattgtc gtcgttgtat gccttgggaa cgtgtatcaa gtgacaatga tatgctgaac
     4261 tttatgaaga ggctgattaa gattcggaaa tacgcgtcag taatcatttc gcatggcaag
     4321 tatagccttc aagaaatcaa atctgatcta gtagctctgg aatggaaata cgaaggatgg
     4381 atcctcaaag caatattcaa ccaatcaaca gaagattatc ttttagagaa agaagcagta
     4441 gcactagcaa gcaattgcca agaattggag aatcagcttg tcatctctcc agatggattt
     4501 gtgattttct aaaaactagt tgatgaagat tatggtacat ttcatatctt atatagtata
     4561 ataaggctag ttactaaact tgtaaaggag aacttaaatg aattgtagag gacatgaaac
     4621 aagacaaaga attgttagag attttgaagt ttagcctaaa gcacatatta agctgttagc
     4681 aaatcaacaa aaacatagtg atgcaggagc aactattgaa gatgaatatt atgtatttat
     4741 cgctgagagt aaaattgatg gcaagaagga agttattcag tgttgcatgg gtgcggcaag
     4801 ggatttttta gaactaatta atcacaaagg gctacctctt tttaatccgc ttgtaggtga
     4861 ttctcatgta aataatagac aagaatatga caatacaggg agtggaaatt tacaacctga
     4921 aaagtggaat gaaactgcaa agcagcttta taatgttata atgtggttga ttattttatg
     4981 gaatgctaag ccggatacac ctttatttaa ttttaaagac gaagtaatta agtataaaac
     5041 atatgagcct tttgaaagca gtataaaatt ggtatcaatc aatgagtaac aaagtatcag
     5101 catgattcga aacatttact tgtaatttgc ggaggaacat caatatggat ggaataacaa
     5161 aagattttat aaaaacggct aaactaatga aacaattatg gccccaattg accgataaag
     5221 aagctattga tgaagtaaaa aaatatacga atggcaaaaa tactgcaatc tttactgaag
     5281 ttgaaggtga cacaattgta ggtctagcac tatgttcact cagatttgat tatgttgaag
     5341 gttgtaaata tagtcctgtt ggattcttag aagggattat tgttgacgag gaatatcgtt
     5401 taaaggatat tgctaaaaat ctctgtacaa aatgtgagga atgggcgaaa aataaaggat
     5461 gtaaggaatt tgcaagtgac tgtactttaa cgaatacgga ttctataaga tttcatctca
     5521 atattggatt tcaggaggca aatagaatta ttcattttaa gaaaaaatta taatttagaa
     5581 acgtgaataa acttgtttaa actttatgta tctttagaaa gtgtgttaac ttgaaagaac
     5641 aaattttttt gatgggtggg aatcccccaa tgaagaaata tagtattgtt gataaaattg
     5701 tattatcaac caagattaaa agaattatca tttttacagt ttttcgagaa aattgggaac
     5761 cgtacatgaa aaagtacacg gaagtttttc aaagtcaatt tcctaatcta aacattgatt
     5821 acttgctcct ggacactgag cagattgatc ttgatagcta tctagatgct gacataatta
     5881 tcatcggtgg tggaaatacg gaaaaatata ttgctaactt atgttaatca ggagttcaaa
     5941 agttatatcg atcatatgct taataaagag gcaaaaatta taggattttc tgcaggagcc
     6001 ctactattag gagaaaaagt ctatgtctca cctaatgata attcagatca tcagataaag
     6061 ataaaaaatg gattaggact ctttagtcag tttttaatta gtgtccatta tgattcctgg
     6121 aatgataaag caaataagga tagagctgaa gaactcgtta atgttcccat aattccacta
     6181 aatgatcatt cttgtcttgt attggataaa cttgaaaaca tcatagagaa aattgactaa
     6241 ctttcataat gtaaaaatgg atctaggaaa taaaagtgta gacgaactgg ataggttact
     6301 agtgaaaact gcaaaaagtg ttgtaaagtg aatcggagtg tgcttgatgg attttagtag
     6361 taagatagcc ataaataaag gctggtcaga tgataaaaaa tattgtgtga cagatcaaaa
     6421 tcagtaaaaa tatttcttgc gtgtttctga taagtcgaag ttagattata aaaaaatttg
     6481 aatttgatat gatggagaaa gtagcttctc ttggagttcc gatgtgtaaa ccaattagca
     6541 ttgaactctg cgatgacgaa gtacattctt tacacgaatg gatagacgga agagatgcaa
     6601 tagatagcat tttagcttat tcagaaaacc aacaatacac atatggtgta gaagcaggaa
     6661 agatacttag aaagattcat acaattcctg ctacagaagt ttgtgaagat tgggaaattt
     6721 ttttaatcta aaaattgatg ataaaatctc caacgaaatg atatggtaag tccgaaacgc
     6781 tcagtgtgta agcttgttgc taaaagcgta aaaaccttgg aacgaaagga ataatagact
     6841 tcctgcgaaa caaaaataca atacaataaa actatggatg atgaagcaag taaacaatta
     6901 agcgatagcc gtttcaagat ccttgtaggt gttcagcgca cgacttttga agagatgtta
     6961 gctgtgttaa aaacagctta tcaacgtaaa cgcgcaaaag gtggacgaaa aagcaaatta
     7021 agcctagacg atctccttat ggtaactatt caatacatgc gagaatagag cacttatgaa
     7081 caaattgcgg ctgattttgg cattcacgaa agcaacttaa tccgtcggag tcaatgggtt
     7141 gaagcaactc ttattcaaaa tggttttacg atttcaaatt ctgccttaat tctgtaaaaa
     7201 cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa atggtataat
     7261 agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta tcctagtaga
     7321 gtggtgatac tatgaagatt agtaagaggc acttattaaa ttattccatc ttgattccct
     7381 acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt gctattttaa
     7441 ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt tggattgtta
     7501 gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga aatgagcgac
     7561 taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt tttattggta
     7621 tttcagtaaa cggggcatac ggttggattt cggttgcagg agtaactatt cagccagctg
     7681 agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa cagcaagaag
     7741 aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc cgtgctttta
     7801 atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc cctgatttag
     7861 gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt ggaatcgctt
     7921 atcgctggtt ttcaaccatt ctggcgctcg tatctgccac ttctgtcttt gtcttgacca
     7981 ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc tatgtagcca
     8041 agcgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt caccagttag
     8101 ctaattctta ttttgccatg gtcaatggtg gttggtttgg tctaggtctt ggaaactcga
     8161 ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct atcgtgattg
     8221 aagaatttgg ctttgttggt gccagtctta ttttagctct cttgtttttc atgattttgc
     8281 ggattatctt ggtcggtatc cgagcggaga atcctttcaa tgccatggtt gcactcggtg
     8341 tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg ggcttgattc
     8401 catctacagg agtgactttc cctttcttat cccagggtgg aaatagtctt ctagtcttat
     8461 cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct aagttgtacc
     8521 gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa aggatagttt
     8581 atgtctcttc aaaaattaga aaattatagt aataaaagtg ttgtgcaaga agaagtcttg
     8641 attctaacag aattactgga agatattact aaaaatatgc ttgccccaga gacctttgaa
     8701 aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct aaaccgtcta
     8761 gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc tatcttacct
     8821 cttttgatta atatttcaga ggatgtggat ttagcttatg aaatcaatca tcaaaataat
     8881 attgatcagg actatttagg taaattatct acaacgatta aattggtagc agaaaaggaa
     8941 aatgccgttg agatcctaga acacttgaat gttgtccctg ttttgacagc ccatccaaca
     9001 caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct tttgcgtaaa
     9061 taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga tttgcgtcgt
     9121 tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa agtgactaac
     9181 gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt acctcatttg
     9241 acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca ggctaaacca
     9301 atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt tacagcaaag
     9361 accttgaagc agtctgcact cactcagtgt gaagtcatca tgaactacta tgataaaaag
     9421 atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt cagcaagcaa
     9481 gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa agagctttac
     9541 cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta tctgattgag
     9601 gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga tttgattgcc
     9661 attcgagatt ctctactaga aaataagggc gagtccttga tttcaggtga ttttgtggaa
     9721 ttattgcagg cagtagagat atttggtttt tacttagcat caattgatat gcgacaagac
     9781 tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat tcattctcgt
     9841 tatagcgagt tgagcgaaga agaaaagtgt gaccttctct tgaaagaatt agaagaagat
     9901 ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa agaattagct
     9961 atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat ccgtcagacc
    10021 atcatttcac atgcaaccag cctttctgat atgctagaat tagctattct gttaaaagaa
    10081 gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt tgaaacaatt
    10141 gaagacttgg atcattcaga ggaaacaatg agaaaatatc tttctcttag ccttgccaaa
    10201 aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc tgacagtaat
    10261 aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca acaattgact
    10321 gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg tggtactgtc
    10381 ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa gtctatcaag
    10441 gatcgtatcc gtttgacgga gcagggtgaa gtaattggga ataaatacgg taacaaagac
    10501 gccgcttact ataaccttga aatgctagta tctgcagcta ttaaccgtat gattactcat
    10561 aagaagagtg ataccaatac cccaaatcgt tatgaagcca ttatggatca agtagtggac
    10621 cgtagttacg atgtctaccg tgatttggtc tttggtaatg agcatttcta tgattatttc
    10681 ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg tccagccgct
    10741 cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt ctcatggtca
    10801 cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa ggaatttatc
    10861 aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg gcctttcttc
    10921 caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa tattgctttt
    10981 gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagac tattttaaat
    11041 gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga actcttagct
    11101 gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa tattctcaac
    11161 tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag tgatcaagaa
    11221 cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc aggttgataa
    11281 ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact attgacaagt
    11341 agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact ttttagagag
    11401 tctgtggtag ctgaaaacag ataagtggca atgatgaaaa ttgggctgaa tgctatttag
    11461 aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt attgcgagac
    11521 tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt cctcacacaa
    11581 gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaaca atggcgtcgc
    11641 ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa tgaaaaataa
    11701 acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct tgatttattc
    11761 ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa ccaagattgg
    11821 tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga tccaagatgg
    11881 acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga actcagaagg
    11941 tgaccaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga atgaccttgt
    12001 ggttggtatc gcaacaccag cagcccaagg gttggctagt gcaacaaaag acctaccggt
    12061 tatcatggcc gctattacag acccaattgg tgctaacttg gttaaagatt tgaaaaaacc
    12121 aggtggcaac gttacagggg tatctgacca caatccagct caacaacaag ttgaactcat
    12181 caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta gcgaagacaa
    12241 ttcaaaaaca caggtcgaag aatttaaggc ttatgctgaa aaagcaggtc tgacagtgga
    12301 aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta tgactagcaa
    12361 ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc caacggttgt
    12421 ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta tggtagaagt
    12481 aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa caggtaaaat
    12541 gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg tcttttcaac
    12601 tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta ttcctgagtc
    12661 tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag gagactcttc
    12721 ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt ttccattatt
    12781 tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt taggatttta
    12841 aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt tgctgtcact
    12901 ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg agcaggttgt
    12961 ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac cttgctctca
    13021 gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg acgtgcgaat
    13081 ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc ggatttgaat
    13141 caactcttga caggtctcat ctttgtgagt attgttattg ctctcatgct ctttttcttg
    13201 gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat ggctagaagt
    13261 ttcgggattc atactggaca catggagctc atgggcttgg tcttatcaaa tggtgtgatt
    13321 gcccttgcag gtgccctcat tgctcagcaa gaaggttatg ccgatgtgtc tcgagggatc
    13381 ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt caagagtttg
    13441 agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca atttttagtg
    13501 tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag tgccttgatt
    13561 ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg agccaagtta
    13621 agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aatcgttaaa aatggctttg
    13681 atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacag gactttatca
    13741 cgattttggg tggaaatggt gctggtaaat caaccctctt taacactatt gcaggaacca
    13801 tatcactaac cagtggaact atccgtattt taggtgagga tgttaccaag ttttcaccag
    13861 agaagcgcgc taagtatctg tctcgagtct tccaagatcc aaagatgggg acagctcctc
    13921 gtatgacagt ggcagagaat ctcctgattg ccaagtttcg tggtgaaaag cgtggactat
    13981 ttccgagacg tctggctagc tataaggata agtttcaggc gaccattgaa aaagtgggaa
    14041 acggtcttga gaaacacttg aatacaccga ttgagttctt atctggtgga caaagacagg
    14101 ctttgagtct cttgatggca accttgaagc gacctgaatt actcttgtta gacgagcata
    14161 ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat gaatttgtta
    14221 agaaagatca attgacagcc cttatgatta cccatcatat ggaagatgct ctcaaatatg
    14281 gcaatcgctt gattgtcatg aaagaaggac gggttatcca agatttgaaa caagaagaaa
    14341 aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg gaaaatgagt
    14401 gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact atgataacat
    14461 ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca atgccaacat
    14521 tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact atctagggct
    14581 ctgtcctttt catggtgaaa aaacaccttc tttcaacgtt gtagaggaca agcagtttta
    14641 ccactgtttt ggttgtggtc gctcaggtga tgtctttaaa ttcatcgagg agtaccaagg
    14701 ggttaccttt atggaggctg tccaaatctt aggtcagcgt gtcgggattg aggttgaaaa
    14761 accgctttat agtgaacaga agccagcctc gcctcaccaa gctctttatg atatgcacga
    14821 agatgcggct aaattttacc atgctattct catgacaacg actatgggcg aagaggccag
    14881 aaattacctt tatcagcggg gtttgacaga tgaagtgctt aaacattttt ggattggttt
    14941 agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc gtgaagaaga
    15001 tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag acacctttca
    15061 caatcgcatt atgtttcccc tgacaaatga ccaaggaaag gtcattgcct tctcaggtcg
    15121 tatctggcaa aaaacagatt cacaaacttc taagtataaa aacagccgat cgactgcaat
    15181 ttttaacaaa agttacgaat tatatcatat ggatagggca aaaaaatctt ctggaaaaac
    15241 tagtgagatt tacctgatgg aaggattcat ggatgttatt gcagcctatc aggctggaat
    15301 cgaaaatgct gtggcgtcga tgggaacggc cttgagtcga gagcatgttg agcatctgaa
    15361 aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc aagccgcgat
    15421 attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgttagca tgcctgataa
    15481 cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct atctattaac
    15541 gaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg aaaacagtga
    15601 aaatctgcag gctcagattg agtttattga aaaaatagct cccttgattg ttcaagaaaa
    15661 gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg cgtcctttga
    15721 ttatgcccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc agaatcgcat
    15781 ggaaggaatt tccagaccga cgccaatcac catgcctatc accaagcagt tatcggctat
    15841 tatgagggca gaagcccatc tactctatcg gatgatggaa tcccctcttg ttttgaacga
    15901 ttaccgtttg cgagaagact ttgcatttgc tacacctgaa tttcaggtct tatatgactt
    15961 gcttggccag tatggaaatc ttcctccaga agttttagca gagcagacag aggaagttga
    16021 aagagcttgg taccaagttt tagctcagga tttgcctgct gagatatcgc cgcaggaact
    16081 tagtgaagta gagatgactc gaaacaaggc tctcttgaat caggacaata tgagaatcaa
    16141 aaagaaggtg caggaagcta gccatgtagg agatacagat acagccctag aagaattgga
    16201 acgtttaatt tcccaaaaga gaagaatgga gtaataatgg caacaaaaca aaaagaagta
    16261 acaacatttg acgtacaggt agcagaattt atccgtaatc ataagcaaaa agggacagca
    16321 acagatgatg aaatcaatgc aagtctggtt attcctttta ccttggacgc tgatgggatt
    16381 gaagatctct tgcaacggat tcaggatgca ggcatttcta tcacagataa cgaaggaaat
    16441 ccaagtgcgc gtgttcttag caatgaagaa gaaccagaac tcagcgatga ggacttgatt
    16501 gggtcaactt ctgctaaggt caatgaccct gtccgtatgt acttgaaaga aataggggtc
    16561 gttcctctct tgaccaatga agaggagaaa gagttggcac tggctgttga agctggcgat
    16621 atcgaagcta aacaacgtct tgcggaagcc aatcttcgtt tggttgtttc cattgccaaa
    16681 cgctatgtcg gtcgtggcat gcagttcctt gacttgattc aagaaggaaa tatgggcttg
    16741 atgaaggcgg ttgacaagtt tgactattct aaagggttca agttttcaac ttatgcaact
    16801 tggtggattc gtcaggctat cactcgtgct attgcggatc aagctcgtac catccgtatc
    16861 ccagttcaca tggttgaaac tatcaataaa ttggttcgtg aacagcggaa tctccttcaa
    16921 gaattggggc aagatccgac accagaacag attgctgaac gaatggatat gacacctgat
    16981 aaggttcgtg aaatcttgaa gattgcccaa gaaccagtat ctcttgaaac tcctatcggt
    17041 gaagaggacg atagccacct tggagacttt atcgaagatg aagtgattga aaatccagtg
    17101 gattatacga ctcgtatcgt cttgcgtgag caattggatg aaatcttaga tactcttaca
    17161 gaccgtgaag aaaatgttct gcgtctacgt tttggactag atgatggaaa aatgcgcaca
    17221 cttgaagatg tggggaaagt ctttaacgta actcgtgagc gtatccgtca gattgaagca
    17281 aaggctttga gaaaactacg ccaaccaagt cgcagcaaac cgcttcgtga ttttattgaa
    17341 gactaagagt gaggaaaata tggcttatac agaagagcaa attgaaaaca tcaaaacacg
    17401 gattttaaca gccttggaag aagtcatcga ccctgagttg ggaatcgata ttgtcaatct
    17461 tggtttgatc tatgagattc gttttgacgg tgacacaggg caaacagaga ttgacatgac
    17521 tttgacaact atgggttgtc ccttagcaga ccttttgaca gaccagattt atgatgctat
    17581 gatagaggtt ccagaagtaa cggatactga ggttaaatta gtctggtatc cagcctggac
    17641 agttgaaaaa atgagtcgct atgcccgcat tgccctaggc attaagtaaa catataaaag
    17701 catgtgagag actgttggaa aagcgaggaa atttcccctc ttttcctcta gtctctcctt
    17761 tcttttgctg attttattca aagaaaatga tataatagta gttatggaga aaaagaaatt
    17821 acgcatcaat atgttgagtt caagtgagaa agtagcagga cagggagttt caggtgctta
    17881 ccgtgaatta gttcgtcttc ttcaccgtgc tgccaaggac caattgattg ttacagaaaa
    17941 tcttccgatc gaggcagatg tgactcactt tcatacgatt gattttccct attatttatc
    18001 aaccttccaa aagaaacgct cagggagaaa gattggctat gtgcatttct tgccagctac
    18061 acttgaggga agtttgaaaa ttccattttt cttaaaggga attgtgaaac gctatgtatt
    18121 ttctttttac aaccggatgg agcacttggt tgtggtcaat cctatgttta ttgaggattt
    18181 ggtagcagct ggtattccac gtgaaaaagt gacctatatt cctaactttg tcaacaagga
    18241 aaaatggcat cctctaccac aagaagaggt agtcagactg cgcacagatc ttggtcttag
    18301 tgacaatcag tttatcgtag taggtgctgg gcaagttcag aaacgtaaag ggattgatga
    18361 ctttatccgt ctggctgagg aattgcctca gattaccttt atctgggctg gtggcttctc
    18421 ttttggtggt atgacagatg gttatgaaca ctataagaaa attatggaaa atccccctaa
    18481 aaatttgatt tttccaggca ttgtatcgcc agagcggatg cgcgaattgt atgctctagc
    18541 ggatcttttc ttgttgccta gttacaatga gctctttcct atgactattt tagaagctgc
    18601 gagttgtgag gctcctatta tgttgcgtga tttagatctc tataaggtga ttttggaggg
    18661 aaattatcgg gcgacagcgg gtagagaaga gatgaaagag gctattttgg aatatcaagc
    18721 aaatcctgct gccttaaaag atctcaaaga aaaggctaag aatatttcca gagagtattc
    18781 tgaagagcat ctgttacaaa tctggttgga cttttatgag aaacaagccg ctttagggag
    18841 aaagtaaaaa gtgaggtaat ctatgcgaat tggtttattt acagatacct attttcctca
    18901 ggtttctggt gttgcgacca gtattcgaac cttgaaaaca gaacttgaaa agcagggaca
    18961 tgctgttttt atctttacga cgacagataa ggatgtcaat cgctacgaag attggcaaat
    19021 tatccgcatt ccaagtgttc ctttctttgc ttttaaggat cgtcgctttg cctaccgagg
    19081 ttttagcaag gcacttgaaa ttgctaaaca gtatcagcta gatattatcc atactcagac
    19141 agaattttct cttggcctgt tggggatttg gattgcgcgt gaattgaaaa ttccagtcat
    19201 ccatacctat cacacccagt atgaagacta tgtccattat attgctaagg ggatgttgat
    19261 ccgaccgagt atggtcaagt atctggttag aggtttcctg catgatgtgg atggggttat
    19321 ttgccctagt gagattgtcc gtgacttgct atctgattat aaggtcaagg ttgaaaaacg
    19381 ggtcattcct actgggattg aattagccaa gtttgagcgt ccggaaatca agcaggaaaa
    19441 tttgaaagaa ctgcgtagta aactagggat tcaagatggt gaaaagacgt tgcttagtct
    19501 ttcgagaatc tcctatgaaa aaaatattca agcagtttta gtagcctttg ctgatgttct
    19561 gaaagaggaa gacaaggtta aactggtagt agctggggat ggcccttatc tgaatgacct
    19621 caaagagcaa gcccagaacc tagagattca agactcagtc atctttacag ggatgattgc
    19681 tcctagtgag acggctcttt actataaagc ggcggatttt ttcatttcgg catcgacaag
    19741 cgaaacgcaa ggtttgacct acttggaaag cttagccagt ggaacacctg tcattgctca
    19801 cggaaatcct tatttgaaca acctcatcag tgataaaatg tttggaacct tgtactatgg
    19861 agaacatgat ttggctggtg ctattttgga agccctgatt gcaacaccag acatgaacga
    19921 gcatacctta tcagagaaat tgtatgagat ttcagctgag aactttggga aacgagtgca
    19981 tgagttttat ctggatgcca ttatttcaaa taacttccag aaagatttgg ctaaagatga
    20041 tacggtcagt cagcgtatct ttaagacagt tttgtatctt cagcaacagg tggttgctgt
    20101 acctgtaaaa ggatctagac gcatgttgaa ggcttcaaaa acacagttga tcagtatgag
    20161 agactattgg aaagaccatg aagaatagaa agaggaacag ctatgaaaaa aacaattaat
    20221 gagaagcggt cgtgataaaa agattgcggg tgtttgtgct ggggtggccc attatctgga
    20281 tatggatccg actatcgttc aagtcatttg gggtgttctt acttgctgtt acggagctgg
    20341 aattgtagct tacattattt tatggattat cgcaccagta gcaactgact attgaaaact
    20401 agctcaattc atgctaagat aatagaaaat agaatgtaac gaaggagaga aaaatggcat
    20461 ttggagataa tggaaatcgt aaaaaaacta tgtttgagaa aataaccttg tttatcgtga
    20521 ttatcatgct agtagcaagt ttattgggaa tttttgcaac tgcaattggt gccctcagta
    20581 atctataaaa tagattcaag aaaatttagt gactgggatt tcccagccct tttttaaagt
    20641 gagaagaaat aatgagtatg tttttagata cagctaagat taaggtcaag gctggtaatg
    20701 gtggcgatgg tatggttgcc tttcgtcgtg aaaaatatgt ccctaatgga ggcccttggg
    20761 gtggtgatgg tggtcgtgga ggcaatgtgg tcttcgttgt agacgaagga ctacgtacct
    20821 tgatggattt ccgctacaat cgtcatttca aggctgattc tggtgaaaaa gggatgacca
    20881 aagggatgca tggtcgtggt gctgaggacc ttagagttcg agtaccacaa ggtacgactg
    20941 ttcgtgatgc ggagactggc aaggttttaa cagatttgat tgaacatggg caagaattta
    21001 tcgttgccca cggtggtcgt ggtggacgtg gaaatattcg tttcgcgaca ccaaaaaatc
    21061 ctgcaccgga aatctctgaa aatggagaac caggtcagga acgtgagtta caattggaac
    21121 taaaaatctt ggcagatgtc ggtttagtag gattcccatc tgtagggaag tcaacacttt
    21181 taagtgttat tacctcagct aagcctaaaa ttggtgccta ccactttacc actattgtac
    21241 caaatttagg tatggttcgc acccaatcag gtgaatcctt tgcagtagcc gacttgccag
    21301 gtttgattga aggggctagt caaggtgttg gtttgggaac tcagtttctc cgtcacatcg
    21361 agcgtacacg tgttatcctt cacatcattg atatgtcagc tagcgaaggc cgtgatccat
    21421 atgaggatta cctagctatc aataaagagc tggagtctta caatcttcgc ctcatggagc
    21481 gtccacagat tattgtagct aataagatgg atatgcctga gagtcaggaa aatcttgaag
    21541 aatttaagaa aaaattgtct gaaaattatg atgaatttga agagttacca gctatcttcc
    21601 caatttctgg attgaccaag caaggtctgg caacactttt agatgctaca gctgaattgt
    21661 tagacaagac accagaattt ttgctctacg acgagtccga tatggaagaa gaagcttact
    21721 atggatttga cgaagaagaa aaagcctttg aaattagtcg tgatgacgat gcgacatggg
    21781 tactttctgg tgaaaaactc atgaaactct ttaatatgac caactttgat cgtgatgaat
    21841 ctgtcatgaa atttgcccgt cagcttcgtg gtatgggggt tgatgaagcc cttcgtgcgc
    21901 gtggagctaa agatggggat ttggtccgca ttggtaaatt tgagtttgaa tttgtagact
    21961 aggagactgg tatgggagat aaaccgatat ctttccgaga tgcggatggt aattttgttt
    22021 ccgccgcaga cgtttggaat gaaaagaaat tggaagaact atttaatcgt ctcaatccaa
    22081 atcgtgcctt gagattggca cgaactaaaa aggaaaatcc atctcagtaa agaagctaaa
    22141 aaatcccggc tctttgtcaa ctgtagtggg ttgaaaaaaa gctaagc
//
